LOCUS       DAOOAI010000001        65989 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_513_length_65989_cov_5.020718, whole genome shotgun sequence.
ACCESSION   DAOOAI010000001 DAOOAI010000000
VERSION     DAOOAI010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 65989)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 65989)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65989
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_513_length_65989_cov_5.020718"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2269
                     /gene="rpoB"
                     /locus_tag="PK345_00005"
     CDS             <1..2269
                     /gene="rpoB"
                     /locus_tag="PK345_00005"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HPX33433.1"
                     /translation="HYGRLCTIETPEGPNIGLISSLCVFARINKLGFIETPYKPVVNG
                     KVDLNAEEVFLNAEEEEDKIIAQANTLLDNDGSFVSERVKVRHLADYPIEEPNNVHYI
                     DMAPNQIASIAASLIPFLEHDDANRALMGSNMMRQAVPLLRPEAPIVGTGIERPIAHD
                     SRVLLTAEGDGIVEYVDADEITIRFTRMEDDRLVSFDDDVKTYKLIKFKRTNQSTAIN
                     LKPIVRKGQKVKKGQTLCEGYATQNGELALGRNLMVAFMPWKGYNFEDAIVISEKVVK
                     DDLFTSLHIEEFVLEVRDTKRGVEELTNDIPNVAAEATKELDENGLIRIGAEVNEGDI
                     LIGKITPKGESDPTPEEKLLRAIFGDKAGDVKDASLKAPPSMKGVVIDKKLFSKVIKD
                     RTTKSKEKDAIEILEKEFKKDTTELTNNLVDKLMILVSGKVSQGVYNNFKEELIPKKI
                     KFAQKQLLGIDYLTVNPHRWTTDKQKNQQIADLINNYIIKYKELLGILNRKKHVAMVG
                     DELPSGIVKMAKVYVARKRKLKVGDKMAGRHGNKGIVAKIVREEDMPFLEDGTPVDIV
                     LNPLGVPSRMNLGQIYETVLGWAGKELGLKFSTPIFDGAEIEDIDEYINKANLPKNGK
                     TYLYDGGTGERFDQPATVGYIYMMKLHHMVDDKMHARSIGPYSLITQQPLGGKAQFGG
                     QRFGEMEVWALEAFGAANILQEMLTVKSDDVIGRARAYENIVKGENMPKAGTPESFNV
                     LVHELRGLGLNITFD"
     gene            2329..6624
                     /gene="rpoC"
                     /locus_tag="PK345_00010"
     CDS             2329..6624
                     /gene="rpoC"
                     /locus_tag="PK345_00010"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HPX33434.1"
                     /translation="MAFRRDSNIVKNDFSRITISLASPDIILERSYGEVTKPETINYR
                     TYKPERDGLFCERIFGPVKDWECHCGKYKRIRYKGTVCDRCGVEVTEKKVRRERSGHI
                     QLVVPVAHIWFFKSLPNRIGALLGLQTKKLEMIIYYEKYVVINPGIKADDGIKYMDFL
                     SEEEYFDIMETVPPENQYLDDNDPNKFIAKMGAEALHDLLARLDLDELSYDLRHKAHT
                     ETSQQRKADALKRLHVVEAFRDARNRIENRPEWMVVKVVPVIPPELRPLVALDGGRFA
                     TSDLNELYRKVIIRNNRLKRLIEIKAPEVIMRNEKRMLQESVDSLFDNSRKANAVKTD
                     GNRALKSLSDSLKGKQGRFRQNLLGKRVDYSGRSVIVVGPELKLNECGIPKEMASELF
                     KPFIIRKMLERGIVKTVKTAKKIVDRKDPVVWDILENIMKGHPVLLNRAPTLHRLGIQ
                     AFQPKLIEGKAIQLHPLVCTAFNADFDGDQMAVHVPLGNAAIIEAQLLMLASHNILNP
                     ANGAPITVPSQDMVLGLYYLTKERKSTPENPVKGEGYRFYSPQEVIIAYNEGKVDLHA
                     IVSVRLPFEENGETVFRLTETTVGRVLLNQVIPPEHGYVNQLLTKKSLRDIIGSVLKK
                     TDTAITAKFLDDIKDLGFRMAFKGGLSFNLDDVIVPVEKEKLIEAANEEVNEVVNSYN
                     NGLITTNERYNQIVDIWTHTNSKLTKALMDQISQDQQGFNPVYMMLDSGARGSKEQIR
                     QLSGMRGLMAKPQKSTASGPEIIENPILSNFKEGLSVLEYFISTHGARKGLADTALKT
                     ADAGYLTRRLVDVSQDVIITEEDCGTLRGLTTTALKKNEEVVEALYDRILGRTALHDI
                     YHPQTNSLIVEAGEEINEEIAQKIEESPIESVEIRSVLTCESKMGVCAKCYGRNLANG
                     RMLQKGEAVGVIAAQSIGEPGTQLTLRTFHVGGVAGGNISAQSTIEAKYDGVLVIEGL
                     RSVPYVSKNDKRYNVVIGRSAEMRIVDQNTGINLASHNIPYGSNLFFMNGDKVKKGEV
                     ICSWDPYNAVIVSEVAGKLRFVNIEEGKTFRVESDEQTGYFEKVIIEARQKIKNPSIE
                     VVDENGEVLRSYDLPVGSHISVDDNQEVKAGDILIKIPRAIGKRSDITGGLPRVTELF
                     EARNPSDPAKVAEIDGVVSFGKKLKRGNKEIIITSKSGEEKRYLVPTTIQILAQENDF
                     VKAGTPLSDGALTPADILAIKGPMKVQEYIVNEIQEVYRLQGVKINDKHFEVIVRQMM
                     RKVEVEDPGDTKFLETELVNKVDFQEENDWIFGRKVIIDPGDSTVFKPGQIVSARKLR
                     DENSSLKRRDKRLVEARDAQSATARQVLQGITKASLQTNSFISAASFQETTKVLTNAA
                     INAKADDLIGLKENVIVGHKIPAGTGLREYEDILVGSKESYDLLIQPKHDDIL"
     gene            6696..7010
                     /locus_tag="PK345_00015"
     CDS             6696..7010
                     /locus_tag="PK345_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HPX33435.1"
                     /translation="MDPKQASQNKLNIELPEDVAEGVYSNLAIITHSRSEFIIDFVKM
                     LPGIPKAKVKSRIILTPQHAKRLLKALQDNVRKFESMHGELKDTDADQMPFNLFGPNA
                     QA"
     gene            7365..8357
                     /locus_tag="PK345_00020"
     CDS             7365..8357
                     /locus_tag="PK345_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPX33436.1"
                     /translation="MIEINIRELNERINQESSFIDLINIEMNKVIVGQKYMIDRLLIG
                     LLANGHILLEGVPGLAKTLAIKSLANVIDAKFNRIQFTPDLLPADLIGTLIYSQKTEE
                     FIVRQGPIFANFILADEINRSPAKVQSALLESMQELQVTIGEQTYKLQEPFLVMATQN
                     PIEQEGTYPLPEAQVDRFMMKVVITYPEKDEEKLILRQNISGTNPTTNKIISPKDILK
                     ARDVVRQVYLDEKIENYITDIVFATRFPADYKLKKFVGMINYGGSPRASINLALASKA
                     YAFIKRRGYVIPEDIRAVAHDVLRHRIGLSYEAEAENITTEDVINEILNMVEVP"
     gene            8431..9306
                     /locus_tag="PK345_00025"
     CDS             8431..9306
                     /locus_tag="PK345_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013547839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HPX33437.1"
                     /translation="MNATEIFKKVRKIEIKTRGLSHQIFSGHYHSSFKGRGMAFSEVR
                     EYQYGDDVRNIDWNVTARYNRPFVKIYEEERELTVMLLIDVSGSNEFGTRMAMKEDVI
                     TEIAAVIAFSAIHNNDKVGVIFFSNKVEKFIPPKKGVTHILRIIRELVDFKTENQDTN
                     ISDALKFLTNAIKKKCTAFVISDFIDNKFADAIQIANSKHDVVAIRIYDPREMQLPPM
                     GMAKFKDAETGRNIWVDTTNSKTRKLYEEWYRNSEKQIKTIFTRSGVDSAHIRTDEDY
                     VRPLINMFKKREARF"
     gene            9408..10364
                     /locus_tag="PK345_00030"
     CDS             9408..10364
                     /locus_tag="PK345_00030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33438.1"
                     /translation="MRFKASIDTTNILIGDQVKYKLDLLIPQDYLFDWPRLTDSLTNK
                     IEIVNMSEIDTIKSAEGVLNVSQVFTITSFDTGFYLIPPIELRYKEISDTDEVSIVES
                     EPFVLNVFSVPVDLSQPIKPIKGPMAAPVTFRELLPWILILLIIGIALYFIITRKKKK
                     IQPVFFKPKPKLLPHIIAFNELEKLKKEKLWQQGLTKDYYTRLSLIIRQYIEDRFSLP
                     AIESTTYDTMQMIRKLTIASDSVLLLENLLERSDLVKFAKAKPLPSEHDQSMDFAESF
                     VRLTAQTVEVRETQNVVDKSHGNDSEVQILDQHSMTNNEPPK"
     gene            10375..11370
                     /locus_tag="PK345_00035"
     CDS             10375..11370
                     /locus_tag="PK345_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPX33439.1"
                     /translation="MFKEFQFANPEYFYILLIIPLMIAWHWFKNNRAKAEIKLSTLLP
                     FASTKKSYRQYLYHSLVVLRIAAIVLLVIALARPQTISSSHDVSIEGIDIVLALDISG
                     SMLAEDFSPNRIEAAKDLAVKFIEGRPGDRMGLVVFAGEAFTQVPLTTDHRVLSDMFK
                     KVKTGMIEDGTAIGDGLATAVARLTESSAVSKVIILLTDGVNNMGVVDPLSAAEIAKL
                     KKIRVYSVGIGTIGTAPYPVQTPFGIRYQNIPVEIDEELLKNISADTHGKYFRATSNT
                     RLKEIYEEIDKLEKSKIDVTEFYKRKEEFLPFAVLAMLIFVVEVLLKYTFFRTTP"
     gene            11389..12435
                     /locus_tag="PK345_00040"
     CDS             11389..12435
                     /locus_tag="PK345_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HPX33440.1"
                     /translation="MQIFRFDNPYFFWALLLIPLFIIIFSLMIFWKKNALKRFGEYQL
                     IRRLMPDVSKNRLIFKFILLMLAFVMMIIGLANPQIGSKLEKVERKGIDLVIAIDVSE
                     SMLAQDIKPNRLQRARQAISRLVDELKNDRIGIVVFAGKAYTQLPITTDYSAVRLFIS
                     NISAESIPVQGTAIAEAINLAVDLFDETETDKAIIVISDGEDHEGDVIESAKNAVKKN
                     IRIYTIGMGLPDGAPIPVIDRYGVQQGFKRDRNNNTVISKLDENMLKEIAEAGMGNYV
                     RANNTQVGLRIIFDEISKLEKTEIESRMFTDYENRFQYFLAPALLLLLIEFLIFERRS
                     SRFRKVNIFGENKL"
     gene            12516..13226
                     /locus_tag="PK345_00045"
     CDS             12516..13226
                     /locus_tag="PK345_00045"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX33441.1"
                     /translation="MTDISLMAQGENRFIRSGNKAFEKGEFNKAEIDYKKALEKNSNS
                     NVGQFNLGSAIHEQKNYDESALIFDNLTHKNLTPDQRAKSFYNLGNSLVNLQQYEQAV
                     EAYKNSLRINPGDEDTKYNLLYAQQMLRQQQQNEQDQDNQQDQNQDQNQQNQEQNDQQ
                     NQDQNQDQNQQNQQNDQQGQPQDSKEDRAQSEPVQISIEDAERMLQALQDRERKTLEK
                     IKKEELKNAKRVKTEKDW"
     gene            13262..15136
                     /locus_tag="PK345_00050"
     CDS             13262..15136
                     /locus_tag="PK345_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatD family protein"
                     /protein_id="HPX33442.1"
                     /translation="MKNHLIKTGYSILFLMALWLLFFNGKLMAQEPQFVASAKAVVVV
                     GERFQLQYRFTAEGVNFKGPIIKDFNTLSGPSPSTSSSVQIINGQVSREVSYTFTYIL
                     EAFKEGKFVIPPATITYNNKQYSSNGLTISVNAGRQAPSSGQSPQQGGQQQQSTADID
                     VFLKAEISNVTPYIGEQIIISYKLYFNNQIAGHDGFQKLSSFPGFWVKDLLANQRDIP
                     TKKEVYKNKQYNVAEVRRFALFPQRAGEIEIIPKETDLTVRVQTASRRRSSDSFFDSF
                     FNDPFFNSRYSDVKKKIVSNGFTINVKPTPTKDKPDNFSGAVGTFTMKSQIDNTEVKA
                     NEPITIKLTVQGAGNIELFDLPNIVFPTDFEVFDPEMKSDIKVANTGVSGTRTFEYLI
                     IPRNPGNFKIKPIEFSSFNPAQGAYNVQRSPEYSITVQRGDGSSSVITYGGTVRQEGI
                     QIIGSDIRHIKLSPYNFSNIGVYFFRSYLYYLLLIIPFLLTIAILIIWRKEMKKRADV
                     ELMRNLKSNKIARKRLKQANIFMKAGNENEFYNEIARASWGYLSDKLNIPVSELSMDN
                     VNSRLLAKGLKEETIQQFIETLKQTEFARFAPGTKDENMERLYSGALEIITKIEKELK
                     "
     gene            15147..15899
                     /locus_tag="PK345_00055"
     CDS             15147..15899
                     /locus_tag="PK345_00055"
                     /inference="COORDINATES: protein motif:HMM:NF019844.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX33443.1"
                     /translation="MKKSLIIVFLVFGSQLFASDNLLLIEQANKYYDESEYSLAVETY
                     ETIINNGFESDKLYYNLGNAYFKLNNLPMAIFYYEKAKKLNPYDADILFNLEIANGRI
                     IDKIEPVPQFFLFKWWDLLVKSRTMEQWARVSIFSFIIFLVMALVFLLARLVWLRKTG
                     FWVGVLSLALFILSFILANQQYNSFNHRSEAIIFTPTVTVKSSPRDNSTDIFVIHEGL
                     KVQLTNQVGDWYEIKIADGSKGWIKENDIKLI"
     gene            complement(15940..17079)
                     /locus_tag="PK345_00060"
     CDS             complement(15940..17079)
                     /locus_tag="PK345_00060"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPX33444.1"
                     /translation="MKNFSRLAITVLSFLVVVGSVSAQKTFYDIKTVKQIKTTPSKDI
                     QRTGSCWANAGTALLEAEWIKNRNQEIDLSEMTFITDLYKLKANAHVSSKGAIYVDEK
                     GLPQDVFTLMQEYGMAPESMYMKSEDKPEKASSGEMDAIIRATLHMVMEKEGGNFTEN
                     WKNTFNASLYRYLGETRINFNYEGKDYTPKSFAAQSGLKASDFVTLTSDDKSAMDTKI
                     TLTAKNNWNKSQAYNVNPDILVSVIQSAVNNGYCTIWYGSLPDEMIYDAEKVAIVPDG
                     KIADLTQGDETDENSFKPLPEKTITNTERQAVLETSAKTEQNYLLISGISKDKNGTEY
                     LIAKNVCEAGDQTLHLSSAFLKLNTGFIVVNKKGLPAGLNSKLGF"
     gene            17595..20780
                     /locus_tag="PK345_00065"
     CDS             17595..20780
                     /locus_tag="PK345_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33445.1"
                     /translation="MKRKLLQLSFLVLIFAMAGLFVAFAYTGNQQPGSSKNAKTDFER
                     ATEYLQQMRNNQITGEINPFDVLNARQQAEKMYFKSGKAFDLDWLELGPDNAPGRVRA
                     LIFDNRDETAKTLIAAGITGGLWKTTNLGATWKKINVANQNLYVSSLAQSADGTIYVG
                     TGEYFCTTNETYFGGLVGTGIYKSTNSDEFTLIEETKPSITALTDTVDWAYINKLVVS
                     KNSGRIFAATNTGLWYSDNGDSWTKVTQYYHDTLTYNVTISIDSTVKCDSFIVEGNNI
                     IMNNPQMNKAVVDTVSYEKTKQTPIRTVMSFAKINCTDVEVGGDGTVAATFGDMVFTA
                     NSNDLIFTNRSGTPSNPNLITREDRDYTTRLIAVDTLNNTADRTVTFNQITNFVADPA
                     LGRPSPLSNNPSRANVAFAPNDPSGDILYAVSTMAGYLYNVYLSEDKGETWRTIFPGG
                     SNTLKPFNGTACYNNTFEVFPDDNYKVLLGGQTMWMGTQVGNGGFFDWGLGPITASLP
                     SGHHVYAFQPGTNNKLVVATNLGIYYGTLGVAYNQFTEISRNLTITQCYTVATGGLKR
                     KVLTGTQGDGTWYISGLGNTPQYGEKIDGGHGGSCVISIINPNAFIFSSSTGTIQRSD
                     DEGQNASMNFSAPSTTLFITPIAFWEDFKSENSRDSITFKARQDYKAGDVILCRSKNP
                     GFGLDAGYPFDYVLETDLAEGDSIRIKDIIQSKLFVANQNTVYLTKDGVKFGKESEWW
                     QIASLVTANGQPTAIAYSKDANYLFIGTKAGFIYRISNIAHAYDYERADIRSPYCIIS
                     TDVIQIPEFENRFITSISVDPNNSNNIIVTLGNYGNEGYVYRSTNALASPWTGITFVD
                     ITGNMPKMPVYSSLIEMKDSNKAIIGTEYGIYTTDEIGKADVTWTAENQGIGLIPVFQ
                     IKQQKIYKDEYVVPSEDPSQPPLVYPRVDNYGSIYIATFGRGTYRADRFMVGIDDIIP
                     TSNKNIETLNIYPNPVDRNTTLSFNLNRNSNIQISIYNLSGLLVNTLNAGTYYAGFNS
                     VTLDCSRLTSGAYLIKIDAENEIMTAKMIVR"
     gene            20816..22303
                     /locus_tag="PK345_00070"
     CDS             20816..22303
                     /locus_tag="PK345_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33446.1"
                     /translation="MKKYFLSTILITAATLIMAQVKVEPPVLIAPANAATKQMPDLIL
                     DWKAVIGALNYELQLSEDEAFTTIVLDSITDLTAVKSSMLKFCQEYYWRVRALYPGNI
                     HSDWSTVWMFTIFSNFDLVKPNDNAENVPPDTELKWKDKVGPLSITGVSYFEVQIDSV
                     DTFDSPEFHEFFVGGNVFKKDMSNLLFGTKYYWRARAGHDKSVSEWSDVRSFTTVGKV
                     TLQRPNNNSVDNDLNVNLRWGEITGILKYEYQIDVDGSFSAPMVELTENIIEPASNLM
                     YGTQYYWRVRARHSRDTTNWSDVWNFTTFNFPTLTSPADNDTGIILRPQLKWDQIKGS
                     IKYEINLSLDEATLSDHKYYKDASDAELPHYNVAPDLEPLTKYFWRVRAFSEIDTSDF
                     SSVWSFTTYTPVGINEYFASSNVNLYPNPVQDAINIEITCNKFTNVDLVIYDLLGQAM
                     LKDNLNLNSGHNQVQIDARTLLNGIYMVNIMYGDQVITKKLVVSK"
     gene            complement(22384..23385)
                     /gene="argF"
                     /locus_tag="PK345_00075"
     CDS             complement(22384..23385)
                     /gene="argF"
                     /locus_tag="PK345_00075"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HPX33447.1"
                     /translation="MAFNLRNRNFLKLLDFTPQEIMFLLDLSSELKKAKYAGTEQPRL
                     KGKNIVLLFAKDSTRTRCAFEVAAFDQGARVTYLGPSGSQAGKKESMKDTARVLGRMY
                     DGIEYRGFGQEIVEELGKYAGVPVWNGLTDSFHPTQILADFLTMREHSIKPLNQLAFA
                     YLGDARNNMGNSLMVGAAKMGMDFRAVAPKELLPAKELVEQCSEIAKTTGAKITLTDN
                     VEIGVKGVDFLYTDVWVSMGEPEEAWNSRINNMIPYQVNMDLIKKTGNPDVKFMHCLP
                     SFHNRETEIGEMIYKKFGIPEMEVSDEVFESDRSIVFDQAENRLHTIKAVMVATLGS"
     gene            complement(23499..24446)
                     /gene="arcC"
                     /locus_tag="PK345_00080"
     CDS             complement(23499..24446)
                     /gene="arcC"
                     /locus_tag="PK345_00080"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF009007.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HPX33448.1"
                     /translation="MNQLAVIAFGGNALLQGGQVGTIDEQEQNAYETCEKLVTLIKKK
                     NYNLVLTHGNGPQVGNLMLNNIAGNKLYGLPEMPLSICVAYSQGFIGYVLEQQLKNVL
                     LQTDLDRDVITIITQVLVNKDDPAFNNPTKPIGPYYTKEEADRIIKESGAVFAEDPRG
                     RGWRKVVASPKPLVINNKKTIENIARQGSIVIAVGGGGIPVYYVSENKLKGIDAVIDK
                     DLASALLAAQINADKLFVLTDVPKVCLNFNTPQEKTLDQITIAEAKRYLEEGHFAEGS
                     MAPKIRAAIYFVEHSGKDAIITKTTSLGVDNGGTRIVMV"
     gene            complement(24730..26511)
                     /locus_tag="PK345_00085"
     CDS             complement(24730..26511)
                     /locus_tag="PK345_00085"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HPX33449.1"
                     /translation="MDDFIVSARKYRPATFDTVVGQDHITTTLKNAIRTKTLAQAFLF
                     TGPRGVGKTTCARIFAKTINCLNLDENIEPCNACESCLSFNRSASFNIFELDAASNSS
                     VNDIRNLVDQVRIPPQSVQYKVYIIDEVHMLSQMAFNAFLKTLEEPPLYAKFILATTE
                     KHKIIPTILSRCQIYDFKRITVRDIAKYLAYVANEEGVIAEPEALQVIAHKADGAMRD
                     ALSIFDQLVSFTGKELNYKLVVNHLNVLDYDYYFKITDFLLSNNIYDTLLLFNKVIES
                     GFDGQHFISGLGAHFRDLLVCTDDKTVDLLEVGEKIKDLYLEQSARCTPTFLINGLDI
                     IQKTDFNYKTSSSKRLHIEIALMQLASINETLSADKKAVLSTDKVEDKNEPIVIPSTP
                     KPQKTLPTTASTQKPAYYQSVTKEKKEITKTKPPQVSETLKLPYIPTTISIKDEVIEK
                     DDGQENEEAENFDFESEEFLNAKTIQTNQLLQVWNDFATKISDANPNLYSTLVSTNPE
                     IEENFIITFEVHNALQEHELNRKKNELVAHLRNELDNPFIQLKILLNTTNVQTRPYKP
                     DEIYQHMLAKNPIVADLKQKFGLSLEY"
     gene            26622..27929
                     /locus_tag="PK345_00090"
     CDS             26622..27929
                     /locus_tag="PK345_00090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPX33450.1"
                     /translation="MIFIYTPKITKRVEYIFKLFFKQLLNIEYEITLKPEIFKQYNGV
                     KINYSNQRFEDSLNFQPVDLLFKTGIDSQELKTIVYKGQKVFFPVYDPKSNLPFDPFA
                     AAFYLVSRYEEYLPYKKDRFGRFDAPESFSFQQNILDKPLVNIWAYWIRNLLLEKFPD
                     LKFRNRTFQFLPTYDIDSAYAYKNKGFVRIAANFARDLINGRLSDMKERFRVIIGKQA
                     DPFDTFDFQFSLQREYDLQPLYFILIANYGEYDKNLPVNNLKFQQLIKSLSDYAEVGI
                     HPSFGSSTSYKLLTSEIERLSRILNREIVISRQHFLRLDFPITYRNLIQADITDDYTM
                     GYASAPGFRAGICDAFNFYDLDMEVETSLRIHPFQVMDGTLRDYMQTDVDGAIEIIRK
                     LIDEVKAVDGTFSSLWHNESLSNKKRWEGWRTLYVDMIRHALI"
     gene            complement(27933..28631)
                     /gene="radC"
                     /locus_tag="PK345_00095"
     CDS             complement(27933..28631)
                     /gene="radC"
                     /locus_tag="PK345_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HPX33451.1"
                     /translation="MKEDKRRQSIKNWSEDDRPREKMMLKGKASLSDAELIAILIASG
                     TKDESAVDLSKRILKSVNNNLIELSKLNVNDLKKFKGIGQAKAITIVSALELGNRRRG
                     AEVIDRKTIKSSRDAFELLQMHISDPNYENFFIILLNQAMKIIKTVNISEGGVTGTIV
                     DPKKVFKHAIENNATSIILAHNHPSGNTKPSEQDRRLTNRLKQGGDLLEIRMLDHIII
                     GMESYFSFADNGEI"
     gene            complement(28708..28962)
                     /gene="rpsT"
                     /locus_tag="PK345_00100"
     CDS             complement(28708..28962)
                     /gene="rpsT"
                     /locus_tag="PK345_00100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004382842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HPX33452.1"
                     /translation="MANHKSALKRIRANKMKQMRNRYHAKTMRNAIRKFKSLTDIETA
                     RDQYPKLVSLIDKNAKRKVIHKNKAANLKSKLSKKLVATE"
     gene            complement(29083..29829)
                     /locus_tag="PK345_00105"
     CDS             complement(29083..29829)
                     /locus_tag="PK345_00105"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPX33453.1"
                     /translation="MKKDLFFVILGLVIIVFASCSNQSKNQPANTTESGEQVTNQTLV
                     QDQPSSSVIHQTPPELPPPGNGGKSSKTAPQTTITQPVKNQDSNPNQTTSTEEIVVGN
                     EVGNDLGDFANYDPDSALIKLSSLNGKMVMVMLWNSLCHHCMVDNEKHREIYNKYHDK
                     NFIHGKGYDIYAIGLDKERETWIKALNEKKYPWKNNVYVIDSWKDRDVRFFGVKNLPG
                     TFLIDRNGIVVNKLFTPAELDKILESYLLK"
     gene            30190..31602
                     /locus_tag="PK345_00110"
     CDS             30190..31602
                     /locus_tag="PK345_00110"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HPX33454.1"
                     /translation="MNFIENKLTALYRRWSGMEPETITQLPQSGSYRQYFRMTGKDKT
                     FIGAYYDQVAENMAFISFSRHFAKMNLNVPEIFAVDDDSVCYLLEDLGDIVLFDYLQK
                     IRSAEGFGEHSLSIYKKILDELVKFQMVAGKSLDYSVCYPASYFDEQAYMWDLNYFKY
                     NFLKLANIPYNEFSLENDFKMLTRLLLTADHHYFVYRDFQTRNILYRNDELYFIDYQG
                     GRKGTLHYDVASLLFQARAEIPFGDRELLLDYYISQAGKIEPGILRNFKDHYYLFVLI
                     RVLQTLGAYGFRGLHEKKQYFISSIPLALINVKWLLENEKIPETLPEIRKVLEATLES
                     NSLDALVKPRLILKINSFSYKRGFPADTSGHGGGFVFDCRALPNPGRYPEYIQMTGLD
                     EPVQAFFSNYPEIDVFIENAYNLVKPAVEVYVSRGFQHLMVSFGCTGGRHRSVYCAEK
                     LAEKLKGVEGVDIFLRHLEQ"
     gene            31751..32494
                     /locus_tag="PK345_00115"
     CDS             31751..32494
                     /locus_tag="PK345_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPX33455.1"
                     /translation="MHSLKQALIFAAGLGTRLRPLTDRIPKALVEINGKPMLERLIEK
                     LIAAGIQNIVINIHHFSKLIIDFVEKHNSFGVNIQFSDEADQLLDTGGGLKKAFSLFD
                     PNLPLLLHNVDVVSNVDLNQLAEFHKESESIATLFVQKRISSRYLLFDENLKLCGWRN
                     YKTGEKILVFNNDDKLIEMAFNGIHIVNPQLVKLITEEGKFSIIPVYLRLALSYKIFG
                     FRNDDAFYLDVGNNENLAKAISLLKKYDV"
     gene            32683..33561
                     /locus_tag="PK345_00120"
     CDS             32683..33561
                     /locus_tag="PK345_00120"
                     /inference="COORDINATES: protein motif:HMM:NF013954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004040 - amidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HPX33456.1"
                     /translation="MSKEEYIKNHRNLAIKEMYENGIPASIKLAQAIIESGNGNSRLA
                     REANNHFGVKCHGWNGDIINIDDDVLQECFRKYSDVEQSYRDHSLFLISRPRYSQLFE
                     LETTDYAGWANGLKQAGYATNPRYAEILIKTIEDFELYQYDVSKSEILASGKLTEEPL
                     DPENIKDKTTSRPLASNRTLLVNNGRKYIFARKGDSFASLAQEFNIYSFQIWRYNNLD
                     KRDKLKEGDMVYLEKKRNKAASPYHIVARGETMRAISQHYGIKLKKLYWYNRMNHGDE
                     PHVGQALWMQQRKPIE"
     gene            33689..35626
                     /gene="thrS"
                     /locus_tag="PK345_00125"
     CDS             33689..35626
                     /gene="thrS"
                     /locus_tag="PK345_00125"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HPX33457.1"
                     /translation="MINITFPDDSKKQYDKGITGLDIAKSISEGLARNVLAAEVNGEV
                     WDATRPINGDSSVRLLTWDDEEGKATMWHSSAHLMAGAIETLFPGAKFGIGPDIEAGF
                     YYDVDTGDRQITDADLILIENKMKEMVKEKHSFTRRQVSKNEAIDYFTKKGDEYKLEL
                     ISELEDGDITFYESGNFVDLCRGPHLPNTGYIKAIKLLSIAGAYWRGDEKRKQLTRVY
                     GITFPKAKELEDYLVMLEEAKKRDHRKLGKELEIFTFSQKVGLGLPLWLPKGAELRER
                     LEQFLKKTQKEAGYLPVITPHIGNVELYKTSGHFAKYGTESFQPISTPIPGEQFFLKP
                     MNCPHHCEIYKSKPHSYKDLPLRYAEFGTVYRYEQSGELHGLTRVRGFTQDDAHIFCT
                     PDQVKSEFFGVMDIVMKIFKALDFKDFITQVSLRDPNNKEKYIGTDENWEKAENAILE
                     VAQESGLETVVAYGEAAFYGPKMDFMVRDALGRKWQLGTIQVDYNLPDRFELEYTGSD
                     NEKHRPVMIHRAPFGSMERFVAVLIEHCAGKFPLWLTPVQSIILPISEKYQEYAEKVL
                     FLLTGLNIRAEIDNRNEKTGKKIRDAEMKRIPFMLIVGEKEEADNTISVRKQGEGDLG
                     TFTIESFSDYIENEIKKLTDK"
     gene            35663..36223
                     /gene="infC"
                     /locus_tag="PK345_00130"
     CDS             35663..36223
                     /gene="infC"
                     /locus_tag="PK345_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HPX33458.1"
                     /translation="MVTPLSSKTPKRFVPRKQENPHRINEEIKGPVVRLVGDNIEQSI
                     ISVKEAIEIAKQHELDLVEISPAANPPVCKLMDYKKFLYDQKKKQKEIKAKAAKVVVK
                     EIRLGPNTDDHDFNFKLNHAKKFLEDGAKVKVEVFFRGRTIVYKDKGELMLLKFAQEL
                     EDYGKVEKLPLLEKKRMIMIIAPKKN"
     gene            36262..36456
                     /gene="rpmI"
                     /locus_tag="PK345_00135"
     CDS             36262..36456
                     /gene="rpmI"
                     /locus_tag="PK345_00135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HPX33459.1"
                     /translation="MPKMKTKASAKKRFTFTGTGKIKRRHAYKSHILTKKSKKRKRNL
                     GYFAIVDNADAKNVKAMLVK"
     gene            36536..36883
                     /gene="rplT"
                     /locus_tag="PK345_00140"
     CDS             36536..36883
                     /gene="rplT"
                     /locus_tag="PK345_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006044808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HPX33460.1"
                     /translation="MPRSVNSVASHARKKKILARTKGRYLAQKNVWTVAKGSYEKGLL
                     YAYRDRRTKKRNFRALWIQRINAGVRPYGLSYSAFIGKLNKSEIKLNRKTLADLAMNH
                     PEAFKAVVDTVKG"
     gene            37009..39378
                     /locus_tag="PK345_00145"
     CDS             37009..39378
                     /locus_tag="PK345_00145"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPX33461.1"
                     /translation="MTKMLSYIRGATFIIVLSLCFVTKLGKAQESAIVAGRVIAQTTK
                     TPIAFADVWLKPGRLNTITNSAGEFEIRGVKSGNYELWVKSVGFESFSQKVEIIENLP
                     FYFSIELKNDVRFIEGVEITGQLTRQGSYLREVVSAKEIMRVPTRDVGEYLRETPNIS
                     GIRKGATNIDPVLRGMKAGQLNVQLDFGQKIEGGCPNRMDPASSHVDPNDLASVEILK
                     GPYALRYGPIFGGIINLVSKKPIPNEGFKIGIKASKGWETNWNGNEEHLSVYGGNEKI
                     YFLMGGNNRYYGNYKAGNGEEVKSSFKKYNYVAKVGVTPHKHHNFLLSYDNSHGVDIR
                     FPALPMDERKDDTKLASLKYHYFNPSKSLQSVDLMVYRSDVLHEMDNKWRPISDTVVG
                     VSKVNALNHGARIDFGVIKNDNTLHFGLDYENIFKDGNRTKNMILQPNLPVKVESLWK
                     NARIENLGVFSEYRRRQSDNMQWIIAGRLDFNSGISDPLALMDMKGNAIYFNDTVSSG
                     FLNFSFSTGLSYIIDNHLTIDFSVGRGTRSPDMLERFIILLPVGYDNYDYLGNPQLKP
                     ESNHQADLTFRVSAGEFGEMTANGFFSYITDYISAMRIPPSVALPQTNSVYGVKRFTN
                     INYALMYGFEFQWETPSTNNWKSNLAVAYTTGINPEAIHFIMENGEVTGSEKVINDPL
                     PELPPFEGNWKLGYNLFNRRFIPELNIRMVAGQHRISKAYDEETTPGFVIFGINMFYR
                     YNRQISISAGVKNLFDTFYYEHLNRRMIGTSASMYEPGRIAYFKIMIDL"
     gene            39383..39742
                     /locus_tag="PK345_00150"
     CDS             39383..39742
                     /locus_tag="PK345_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33462.1"
                     /translation="MKRIFWLFLLLVLVLQACEMRSKKSVKYVATDAISGYDISYRNE
                     SGNLVKKRVEVNSALERWEYSFIAEQGDIVYVSGNYKDVASALKIMILIDGKIFKQAA
                     TRHDTVRYVTVSGVIPY"
     gene            39839..40633
                     /locus_tag="PK345_00155"
     CDS             39839..40633
                     /locus_tag="PK345_00155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33463.1"
                     /translation="MRQYIYLLIALLLVSCNGSKDDPELDMGHITFHFTHYADDDLLI
                     FDELIYTNAAGNQYMVNEIQYFISDVKLHRRDGTYQLIEEWKDIHYVDTDLLETQSWK
                     VFDKIKPGGYDKISFVFGISESKNKSLMFVNPPERDMFWPEVLGGGYHYMKLNGKWLT
                     PEQQVFPFNFHLGIGQIYAGNVIHVDSIIGFVQNYFEVQLANSSFEIGAGDTLQFEIR
                     MDVQNWFQHPHIYDHNQWGGDIMQKQEAMQIGCENGKVDVFSILRK"
     gene            complement(40757..41977)
                     /locus_tag="PK345_00160"
     CDS             complement(40757..41977)
                     /locus_tag="PK345_00160"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPX33464.1"
                     /translation="MKLIIQHTILILLLLSSLVSFSQRNADLLTKHHKQVAVASNKSE
                     DSKNLTANFITLDRLGSAPMTVQFKDASEGQPTGWNWNFGDGNSDTVKNPLHVYQEPG
                     NYTVKLSITKGSESSSLTRNNYIVVVSGSSCDTLYYPLAGDYTLYVISQNGSGYVSGN
                     NSYGDLAKASYFQNNSESEIKLIGGIFDFAVAKRSMASDKQITFKAWDCNGEGGSPGK
                     TLAENTLFLSHIVQEVGFGWSTLLFFNDPPEMNQPFFLGVELPKNYGDTLALYTNLDG
                     DVIDGNGWEQHDNGNWYPYSNQQFSWNINIDHAIFPLMHYSTGISNHILNTQLLVYPI
                     PASDMLNIRFLDPADTNALISLTNLTGKTELQIVNTENSNISLPVDQLKPGIYLLRIE
                     TKSSISNQKIIIVR"
     gene            42716..43732
                     /locus_tag="PK345_00165"
     CDS             42716..43732
                     /locus_tag="PK345_00165"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HPX33465.1"
                     /translation="MCKIFIFRISIFFAIVFVSQGLFAQEYQKVEAESGEGIYSLLRR
                     FNLDPKDYYNTFLEINKNRLNPDLQLLKGKTYFLPIVVDTTALETERQIGVFPIFGKK
                     YEKVEFKDDKLQGAVFYIISGHGGPDPGAMGKKNGHTLCEDEYAYDVCLRLARNLIEH
                     SATVYIITQDPDDGIRDEEYLKCDKDEMCLGNKKIPLNAVERLKQKVETVNQLFEKHL
                     GQYQRKLEIHVDSRYSNQKVDIYFYYHPGSNKGKQLGETLLKTIHDKYKQHQPNRGYK
                     GTVTERKLYTLKNTKPVSTYIELGNINHPRDLERLIIVDNRQAIANWLTLGLIKDFEN
                     SKRR"
     gene            complement(43763..44995)
                     /gene="hydF"
                     /locus_tag="PK345_00170"
     CDS             complement(43763..44995)
                     /gene="hydF"
                     /locus_tag="PK345_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster maturation GTPase
                     HydF"
                     /protein_id="HPX33466.1"
                     /translation="MNLNSPVIKPRESTPHIGIFGKTNLGKSSIINILTGQQTSIVSE
                     TPGTTTDPVKKRMEIPGIGPVILVDTAGIDDIGELGAKRIERSIEIVKQIDIALLLIS
                     DNTIGMSEKKIIGYFYDYGVPFLWIHNKSDITPLDKDFAKHLESEYKQPIIEFNALQP
                     NNLEYLLQKIRSIMPETVWSRPTLLGELINKGDVVLLITPIDIAAPEGRLILPQVQTI
                     RDILDHDSVAVVVKEHEAESFLKKTAVEPALVITDSSIFLEASKIIPETIPLTGFSIL
                     LARYKGPFDAYLNGTPKISSLKEGDIVLILESCTHHVSCEDIGRVKMPNWMDKFTGKK
                     LKYEVVSGLNQLPRPITDYALVVQCGGCMITRKQMVNRLKPAIDAGIPVTNYGMAIAY
                     MQGVYQRAILPFIKKPLN"
     gene            complement(45019..46368)
                     /locus_tag="PK345_00175"
     CDS             complement(45019..46368)
                     /locus_tag="PK345_00175"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007656159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HPX33467.1"
                     /translation="MNYLIANTNIVNEGRIFKGSVFTKGEEIEKILEHTDDIEVNSFK
                     GYEFIDASNMFLLPGVIDDQVHFREPGLTHKADIGSESRAAIAGGVTSFMDMPNTLPN
                     TLTQDLLEEKYKLASEKSLANYSFYMGASNDNVKEIVKTDPVNVCGIKVFMGASTGNM
                     LVDNKSTLQAIFKEAPCLVATHCEDEATIQNNIVRLRNIYRDDIPIHYHPQIRSDEAC
                     YISTSLAIELAEKYNTRLHILHLSTALETTLFRNDIPLPEKSITAEVCIHHLWFNDEA
                     YHTKGNLIKWNPAIKTETDRRALWKALLDNRLDVIATDHAPHTLVEKRNSYFKTPSGG
                     PLIQHSLIALMEFFHQGKISMEKIVEKMCHAPAICFKVNKRGFIRSGYKADLVIVNPN
                     QPGIVEKSNLLYKCGWSPFEGTLFQSAVTHTFVNGNLVYEMGKFDLSARGQRLTFNR"
     gene            complement(46457..47419)
                     /locus_tag="PK345_00180"
     CDS             complement(46457..47419)
                     /locus_tag="PK345_00180"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HPX33468.1"
                     /translation="MGTITRTNFNFDGQKSLYTGKVRDVYNINDELLVMITTDRISAF
                     DVVLPKGIPYKGQVLNQIAALFLDATKDIVPNWKISVPDPIVTIGLHCDPFPVEMIIR
                     GYLTGSAWRTYQKGIRQICGVPIPDGMKEHQQFAEPIITPTTKATSGHDEDISKEDII
                     NQRLVSEEDYALVEKYTRELFKRGTEIAKKKGLILVDTKYEFGKRDGKIYLIDEIHTP
                     DSSRYFYLEGYNERQKKGEPQKQLSKEFVREWLLANNFKGDRGEKIPKMTDQIVSSIS
                     ERYIELFERITGESFQKANASDILKRVENNITSFLKNYYRNKKN"
     gene            complement(47397..48386)
                     /locus_tag="PK345_00185"
     CDS             complement(47397..48386)
                     /locus_tag="PK345_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPX33469.1"
                     /translation="MTEKNILIESVNPLDVFGVNDSNFKILKKHFPKLKLIARGTEIK
                     VIGDEAEIISFEKKFALLILHLERFGKLSEYDVNDLLSSESDYTPQDLQFGSDVLVHG
                     RNGKLIKARTPNQQRMVDSCRRNDLIFAIGPAGTGKTYTAVALAVQALRNKEIRRIIL
                     TRPAVEAGENLGFLPGDLKDKLDPYLQPLYDALRDMLPSQKLLGYLEEGTIEIAPLAF
                     MRGRTLDNVFAILDEAQNATENQLKMFLTRMGASAKFIVTGDITQIDLPRNQSSGLIQ
                     VTRILNNIPGIDFIYLNEKDIIRHKLVVQIINAYSNQPKTGNNKQNNGNHNKN"
     gene            48458..49297
                     /locus_tag="PK345_00190"
     CDS             48458..49297
                     /locus_tag="PK345_00190"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HPX33470.1"
                     /translation="MACQGNLIFARDLKIRHLGFMALIALLSDWGTKDHYVGMVKGKI
                     LTKIPESDIIDISHEIPPFNLNNAAFVLRHCYRQFPEKTIFIVDITSEATIEMPHVVV
                     EYAGSYFIGADNGIFSLAFDEDPDSITEIDIYQDTHTFTFAAFDLFVKVAQHIVEGKE
                     ISLLGPKRDRLAERIQLKPVAEPDLIKGHVTYVDNYENVFTNIDQELFLRIGQKRSFR
                     ILFGASRYSVRQISQSYKDVSEGEIVALFSSSGFLQIAVSMGNASGLLGLKVNDMIRV
                     EFG"
     gene            complement(49611..50651)
                     /locus_tag="PK345_00195"
     CDS             complement(49611..50651)
                     /locus_tag="PK345_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPX33471.1"
                     /translation="MKLTTYIFIITALLTLPTHAQNDKQYKVGCIAFYNIENLFDTIN
                     QPGVIDEEFLPESTIQWNTKKYFEKLDRISEVISKIGTDLTPDGPAIIGLSEIENRIV
                     LDDLVAQEHLRRQNYQIVHYDGPDLRGVDCALLYNPKYFTVTNSESKRLIIPGMEMFF
                     TRDQLVVSGMFDGEMMHFIVNHWPSRRSGQKKSEPLRMAAAQLTRSIVDSLLALDPNA
                     KVIVMGDMNDDPKDKAIRKGLNTVGVVKKIKPGTLFNPMESLYRQGIGSLAYRDSWNL
                     FDQIILTPALIGDDKSTYTFYRAGVFNKPWLIQKEGAFKGYPWRTFAGGVYLGGYSDH
                     FPVYIYLIKEKE"
     gene            50788..53295
                     /locus_tag="PK345_00200"
     CDS             50788..53295
                     /locus_tag="PK345_00200"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPX33472.1"
                     /translation="MKNFLLLSALSFLTFNLFSQVDTTVIKSEEVNLNQVYMITSADL
                     ESDEQPQDISGLLQSSRDIFISTAGFTFGQTRFRIRGYDSENTTVLLGGVPLNDMETG
                     RAYWSAWGGLNDVTRSQEVNFGISASPYSFGGISGSVNIESRASKQRPGTRLTYSSTN
                     RAYRNRIMATYNSGMMDNGWAISVSGSRRWANEGYVEGTFYDAWSYFLALEKRINNKH
                     SIGFVGLAAPSKAGRSGPVTQEANDLAGTNYYNPYWGYQNGEKRNARVGNYNQPKLIL
                     TDYWELDKSTKITTSAAYFFGRYSTTALEWFDAPDPRPDYYRKLPSYWKDNPQKFNYL
                     TEQWKNNEQFRQINWDFFYFANSKNLYTIEDAYGIAGNNITGNFSKYVVEDRRNDHNH
                     FMINSNLWKEINHNIILNAGINLSFYKQRSYKVLDDLLGGDFYIDLDKFAEQDYFDPY
                     QGQNDLRTPNRPVYEGDSFGYDYDANINNYNGFVQADFKYAKMDYFIAATISHTSFWR
                     TGHMQNGKFPTESLGEAEKQNFFNFGVKGGLTYKITGRHFIVGNAMYLTRAPYFRDSY
                     ISPRTRNHVLSGLINEKIVGGDIGYVLRAPKIQARATLYYTEFKDQVYSRSFYHETLR
                     SFVNYQMTGVDTKSMGAEIGVDYKLTSTINITGVAGLGRLTYNSRPLVTISQDNNSEI
                     LAENRMVYLKNYYVGGNPQTALSGGIKYNAPKYWWIGVDFNYFDDIYLEPNPDRRSEA
                     AAGIYNSEDLRFEQLLAQERLSSAYTIDLFGGKSWRVKKYYIVFNLSINNLLNVTDFA
                     FGGFEQFRFDPNNIDKFPPKYFYLYGTQYFANLSLRF"
     gene            53332..55431
                     /locus_tag="PK345_00205"
     CDS             53332..55431
                     /locus_tag="PK345_00205"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6359 domain-containing protein"
                     /protein_id="HPX33473.1"
                     /translation="MIKRLIFPVLIIAVILLFGLTSCVKQDFDKPPSKDIPVGKVITI
                     KELTSMYQGQPIVFDTAMSTYGVVVGDESSGNIYKNSYVQDSTGAINLRLINPGGLYV
                     GDSIRIYLKGLTLSDYGGVRQLDSVDVDLNIVKLKTKINIEPKVVTIPELAGGKYNSQ
                     LIMLENVEFSAADTAKTWSDPHGLTTQNRSLMDCDNNVVVVRTSGYANFAGVSLPNGN
                     GTFVAIASIFNNTIQLYVRDYGEINLVGERCTGGGGGGGGQGSGTQSDPWNVAYGIAN
                     QNAMPYEVGWVKGYIVGSVKSGVTSITSNDDILYSPPFNLATNVLIADSPDEKNYQNC
                     VIVNLPAGKPLRSEVNLVDHPDNLGKWLNVSGTLRTYFGAAGLRDSNGESSDFELEGG
                     GGGGGGGTGSGTQSDPYNVEFGIANQNASPYIVGWIKGYIVGAVKSGVTSISSNDDIL
                     YDPPFDLATNVLIADSKEERNYQNCVIVNLPAGKPLRFDVNLVDHPENLGKWLKVKGT
                     LRTYFGAAGLRDSSGEASDFELEGGGGGGGGTAFFSEDFNTNLGVFTAYSVTGDQVWG
                     WGNFDGGCAVMSGYVQGTNYPNEDWLVSPAISLAGKTGVKIEFREAINYITNISDMKV
                     LISTDYSGSGDPSINGTWTELQGFNRAPGNNWNFVNSGEVSLASYENKTVFIAFKYLS
                     YSSGSATWEVSKVELRD"
     gene            55452..57344
                     /locus_tag="PK345_00210"
     CDS             55452..57344
                     /locus_tag="PK345_00210"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPX33474.1"
                     /translation="MRTCLAILFFFITIIGQGQPVGYYNGTEGLRGSALKTKLHEIIC
                     GHNSLSYYFSKYVIYYADADPEIPGNVILIYTGRSQDGFDYGIGGNQLNREHVWAKSH
                     GHFSGILPMDSDVHNLKPVDASVNSSRSNLDFDYSLYPHPEATECKFTPGVSWEPRDA
                     VKGDVARTIFYMDARYEWTNGEMNLTVVDQVNTYPQPEHGKLSALLEWNEMDLPDLFE
                     YNRNNVVHRFQKNRNPFIDNPDFVGLIWGDKSLPYFSIGDIALSDDQPYEDESVVLYC
                     SIYPSPATDKVKVMVGSDFNEFDYEIMMTFNNGLWQADLLPNEEGEVVYFAVKAGDGA
                     NLSISPTYSYRVAAAWGEPITSIQEIQGTGDQTPYQNIQVTTTGVVTSFLPTGYFIQA
                     GQGPRSGLFVYDPSRYPSIGDSIVVSGIATEYYGLTEITNVSMYKLIKTDRKMPAPEV
                     LDSNQIGEDWEAVLIRIENAECTFTQHWNNSGMWRVSDDYGQVNVQNNDVFSIDPVLN
                     ERYTITGPLNYQNSNWKIELRYLYDVAEPASVGEKTPTVKLAIYPNPSNETVTIAIPP
                     NRGKNPVIRILDILGNEKWIIPVDPHDNLLVLNLLELNLTKGVYFVIFVDDQIQISEK
                     LIYLPN"
     gene            57404..58141
                     /locus_tag="PK345_00215"
     CDS             57404..58141
                     /locus_tag="PK345_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005648956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HPX33475.1"
                     /translation="MSEKLVVIPTYNEKENIEAILTYVLTMEGDFHVLVVEDNSPDGT
                     ADIVKSLIKKYPDRLFIEERKGKLGLGTAYIHGFKWALNRQYDYIFEMDADFSHNPDD
                     LHRLLDACESGADVAIGSRYVGNVIRVVNWPMGRVMMSYYASKYVRFVTGLKITDSTA
                     GFICYRRKVLETIWFDRIKFIGYAFQIEMKLTASKLGFNVVEVPIVFTDRTVGTSKMT
                     KGIFREAVLGVIKLRFKKILPVPSNKN"
     gene            complement(58138..59007)
                     /locus_tag="PK345_00220"
     CDS             complement(58138..59007)
                     /locus_tag="PK345_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside phosphorylase"
                     /protein_id="HPX33476.1"
                     /translation="MKRIKDSELIINSDGNIYHINIKPGILAENIILVGDPARVSKIS
                     RRFSKIEHQSQNREIITNTGIYNGIPISVVSTGMGTDNIDIIINELDALANIDFKTRE
                     PKKSFTKLNLIRIGTSGAIQPDIAIHSIVASTHGVGLDGLLYFYNSDHVIEKELTKEF
                     IAQTGSFSNLPKPYIVSCSEKLMKKVGHDLFQGITATAPGFYGPQGRTIRIPLRHVEL
                     SEKIKNFSFERHRVLNFEMETSALYGLGKLLGHDALTICAVIANRATGDFAVDYNKTI
                     DEMIGLVLDRITS"
     gene            59083..60318
                     /locus_tag="PK345_00225"
     CDS             59083..60318
                     /locus_tag="PK345_00225"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HPX33477.1"
                     /translation="MEAEIITIGDELLIGQVVDTNSAWLAEQLNLLGIKVHQITTISD
                     SHEEIIKTLTEVSRHADIILLTGGLGPTKDDVTKLALCEFFDTYLVFNQDAYENVERL
                     FRQRGFLMTEINRQQASIPAACTPIPNPEGTAPGMWFEKDGKIFISMPGVPFEMKSIF
                     INQIAPKLAKVNNTLYILHKTILTHGVGESFLAQTIEAWEDNLPSNIKLAYLPQPGVV
                     RLRLSAVGNNKSLLENQLNEEQVKLLHIISDLVFGFDNDSMEGVVGQLLRMSGKTLST
                     AESCTGGYIAHLITSIPGSSFYFKGSVVAYSNEVKVSQLNVARQAIIDNGAVSEVVVR
                     QMAEGVRKNLQTDYSIAVTGVAGPDGGSEEKPVGTTWIAVATSEKVVSHRFHFGEHRD
                     RNIRRAALAALNMLRQELK"
     gene            complement(60338..62386)
                     /locus_tag="PK345_00230"
     CDS             complement(60338..62386)
                     /locus_tag="PK345_00230"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPX33478.1"
                     /translation="MDNTTISFLNSGKKTIASSALVVLINSKRMSFLYLKLASIIRSL
                     LVVIFLVSSIYVSLAQTLTTTSKKAIRLYHEGRNHFALLEYEQAELKLKKAIGTDKNF
                     LEAYMLLGDIYRTQNKNIYAVETYKTAIEINPDKYPEVFYFYGILCFEMQQYQLCIEQ
                     LEQFKNYNIINEARQREADYYLASARFAQRSLKNPVPFQPINLGTNINSANDEFINAV
                     STDELKIYITAEEAVQGLVGVSDRFFISYRDDLNSAWQPIALLPPPLNSEDTQGALSL
                     THDGRYLLFAGCQWSDGYGSCDIYAVKTVGKNIGEPQNLGHTLNSTAWDSQPCLAPDG
                     RTLYFSSTRKGGYGKSDIWRSYLQDNGEWSTPENLGETINTYGSEMSPYIHADGKTLY
                     FSSDRHPGMGGMDLFVAKMDSAGNWEKPVNLGYPLNTSGDEINIIVNARGDKAYISAR
                     LPEGNGGFDIYEFEVYPEIRPKPSTYVKGIVVDAKTNNPLLSYFTLTEIDAGKDIITS
                     FSDEKTGEFLLCLPINRDYALNVSREGYLFFSEHFSLTDYKGVFEPYLLNIALNPIIP
                     GEVTIMRNIFFGSGKANLKDESLAELNKLYEFLIQNPSIAVEISGHTDDVGSEDYNMT
                     LSDQRAQAVTDFLVAKGIDSNRLQHKGYGFTKPLTTNQTQEGRAKNRRTEIKVIEMPK
                     "
     gene            complement(62595..63245)
                     /locus_tag="PK345_00235"
     CDS             complement(62595..63245)
                     /locus_tag="PK345_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-carboxy-7-deazaguanine synthase QueE"
                     /protein_id="HPX33479.1"
                     /translation="MLNPKFISYEQKRHFEEGKLLPVMEAFYTLQGEGYNTGEAAFFI
                     RIGGCDVGCRWCDIKESWNPEIHPLTSTLQVVEQAAAYPSKAVVVTGGEPLMYNLNYL
                     CAELKKNSIKTYLETSGAYPLSGQWDWICLSPKKNSAPIDSITSIANELKVIIFNDGD
                     LTWAEKYANLVSNKCKLYLQPEWSRSKFMTPVIIEYIKKYPKWKISLQSHKFMNIP"
     gene            complement(63312..64190)
                     /gene="folD"
                     /locus_tag="PK345_00240"
     CDS             complement(63312..64190)
                     /gene="folD"
                     /locus_tag="PK345_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HPX33480.1"
                     /translation="MQIIDGKAVAAAIKEEIKAEVAQIIDNTDESPHLSAILVGHDPA
                     SETYVASKEKDCKSVGMMSSVYRLSERTTESELLETINFLNQDEEINGFIVQLPLPRH
                     INVEKIIGSIDPKKDVDGFHPYNLGKMMLGQESFFPATPYGIVELLKRYKIDTAGKHC
                     VVLGRSNIVGTPLSVMLSRKSDPGNCTVTICHSQTRNIKEHTTQADILIAAIGQPEFV
                     TADMVKEGAVVIDVGIHRIADQSKRSGYSLTGDVNYNEVAAKCSYITPVPGGVGPMTS
                     VALLMNTLKAFKANRK"
     gene            complement(64353..65705)
                     /gene="ffh"
                     /locus_tag="PK345_00245"
     CDS             complement(64353..65705)
                     /gene="ffh"
                     /locus_tag="PK345_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HPX33481.1"
                     /translation="MFESLSEKLDRSFKILKGQGKITEINVSETLKEVRKALLDADVS
                     YKIAKQFTDEVKERALGQNVLTAISPGQLMIKIVHDNLTELMGGEHTDINIKGDPAII
                     LIAGLQGSGKTTFSAKLANYLKQKKGKRPLLVAGDVYRPAAINQLQVLGNTIDVEVYT
                     EEGSMEPVKITRNSINHAKANGYNVVIVDTAGRLAIDEQMMDEISSIKEALKPQETLF
                     VVDAMTGQDAVNTAKAFHDRLNFEGVVLTKLDGDTRGGAALTIKAVVSKPIKFVGIGE
                     KIDALDVFYPKRMADRILGMGDIVSLVEKAQEQFDEQEARKLQKKIAKDQFNFNDFLA
                     QIKQIKKMGNVKDLMSMIPGMGKAIKNLDIDDDAFKHVEAIIYSMTPSERDNPKIING
                     SRRKRIALGSGTDIQEVNRLIKQFDETRRMMKMMTTSGGKGMMKMMQNMQGGKKINIK
                     "
BASE COUNT        19994 a        11711 c        13650 g        20634 t
ORIGIN      
        1 acattatgga cgattatgta caatcgaaac acctgaagga cctaatatcg gactgatctc
       61 atcactttgt gtttttgcca ggattaataa acttggattt attgaaacac cgtataagcc
      121 tgttgttaat ggaaaagttg atctcaatgc agaagaggta tttcttaatg ctgaagaaga
      181 ggaggataaa atcattgcac aggccaatac gttgcttgac aatgatggat cttttgtaag
      241 tgaaagagta aaggtaaggc acttagccga ttatccgatt gaggaaccta ataatgtcca
      301 ttatattgat atggcgccaa atcagatagc ttcaatcgct gcctcactta ttccttttct
      361 tgagcacgac gatgctaacc gtgcattaat gggatcgaac atgatgcgtc aagcagtgcc
      421 attattgaga ccagaagcgc ctattgtcgg aaccggtatt gaaagaccta ttgcacatga
      481 ttcaagagta cttcttactg ctgaaggcga tgggatagtg gaatatgttg atgctgatga
      541 gattacaata aggttcacaa ggatggaaga tgatcggctt gttagctttg atgacgatgt
      601 aaaaacttac aaactaataa agtttaaaag aacaaatcag agcactgcaa tcaacttaaa
      661 gccgattgtg cgtaaaggac aaaaagtaaa aaaaggacaa acactttgtg aagggtatgc
      721 cacacaaaat ggcgaattag cacttggccg aaatttaatg gttgcattta tgccgtggaa
      781 gggttataat tttgaggatg caattgttat atctgaaaaa gttgtaaaag acgatttgtt
      841 tacctcactg catattgaag aatttgttct cgaagtgcgt gatacaaaac gcggtgtaga
      901 agagctgaca aacgatatac caaacgttgc tgcagaagct accaaagagc ttgatgaaaa
      961 tggccttatc agaataggag ctgaagttaa tgaaggtgat atcctgatag gaaaaataac
     1021 accaaaaggt gagtctgacc caacaccgga agagaaattg ttgagagcca tttttggcga
     1081 taaagccgga gacgttaaag atgcttcatt aaaagcgccg ccttcaatga aaggtgttgt
     1141 gattgataaa aagctattct caaaggtgat aaaagaccgg acgactaaaa gtaaggagaa
     1201 agatgcaata gaaattcttg aaaaagaatt taaaaaggat actacggaat taacaaataa
     1261 tttggtggac aagctcatga tccttgtgag cggtaaggtg tcgcagggtg tttataataa
     1321 ttttaaggaa gagttaatcc cgaagaagat taagtttgct caaaagcagc tccttggcat
     1381 tgattatctt actgttaacc cacatagatg gactacagat aagcagaaaa atcaacaaat
     1441 tgctgatcta atcaataatt atattattaa atataaggaa ctccttggaa tattaaacag
     1501 gaaaaaacat gttgccatgg ttggcgatga attgccaagt ggtatagtga agatggcaaa
     1561 ggtttatgta gctcgaaaac gtaagcttaa agttggcgat aaaatggcag gtcgccatgg
     1621 taacaaaggc attgtagcta agatagttcg tgaggaggat atgccttttc ttgaagatgg
     1681 aaccccggta gatattgttt tgaacccatt aggagtgcca tcccgaatga accttggtca
     1741 gatttatgaa acggttttag gttgggcagg taaggagtta ggattgaaat tttcaactcc
     1801 aatttttgat ggagcagaaa ttgaagatat tgatgagtat attaacaaag caaatctccc
     1861 gaaaaacggc aaaacttacc tttatgacgg aggcactgga gagcgctttg atcagcctgc
     1921 tactgtagga tacatttaca tgatgaaact gcatcacatg gtagatgata agatgcatgc
     1981 acgttccatt ggaccttatt cacttattac acagcagcct cttggtggaa aggcacaatt
     2041 tggcggtcaa cgttttggag agatggaggt atgggcgctt gaagcattcg gcgctgccaa
     2101 catactacag gagatgctta cggttaagtc tgacgacgtc atcggtcggg cacgtgcata
     2161 cgaaaatatt gtgaaaggtg aaaatatgcc aaaagccggc acaccggaat cattcaacgt
     2221 attggttcat gaattgcgcg gcctcgggct taatatcact ttcgattaat aattttattg
     2281 taaattacag tttttagatt ccaattatat ttaaaaaaat atccataaat ggctttcaga
     2341 agagatagta acattgtaaa aaatgatttt tcaagaatca ctattagttt agcatctcct
     2401 gatattattt tggagagatc ttatggagag gttactaaac cggaaacaat caattaccga
     2461 acctataaac ccgaaaggga tggtttattc tgtgaaagaa ttttcgggcc ggttaaagac
     2521 tgggaatgtc actgtggaaa atataagcga atacgttata aaggcactgt atgtgatcgt
     2581 tgcggggttg aagttactga gaaaaaagta cgtcgcgaac gatcaggaca tattcaactc
     2641 gtggttcctg ttgcgcatat ttggttcttt aaatcattgc ctaatagaat tggtgcctta
     2701 ctaggtcttc agacaaagaa gcttgagatg atcatttact acgagaagta tgtggttatc
     2761 aatcccggta ttaaagcaga tgacggtata aaatacatgg atttcctttc cgaagaggaa
     2821 tattttgata taatggagac tgttccaccg gaaaaccaat atcttgacga taatgatccg
     2881 aataaattca ttgcaaaaat gggagcggaa gccttacacg atctgttggc acgtctcgat
     2941 ttggatgagc tttcgtatga tttacgtcat aaagctcata ccgaaacttc tcagcaaaga
     3001 aaagcagatg ctctgaaacg attgcatgtt gttgaagcat ttagagatgc aagaaatcgt
     3061 atagagaacc gccctgagtg gatggtcgtt aaggtagttc ccgttatacc tccagaactt
     3121 aggccgcttg tagcccttga tggagggcgc tttgctacat ccgaccttaa tgagttgtat
     3181 cgtaaggtga taatacgaaa taacaggctt aaacgactta ttgaaataaa agctccagaa
     3241 gtaataatgc gcaacgaaaa acgtatgttg caagaatctg ttgattcatt attcgataat
     3301 tcaagaaaag ctaatgctgt aaaaacagac ggcaacagag cgttaaaatc actgagcgat
     3361 agcctaaaag gtaaacaggg tcgtttcaga caaaaccttt tgggaaaacg tgttgattat
     3421 tccggtcgtt cggttattgt tgttggccct gagttgaaac ttaatgaatg tggtatcccg
     3481 aaagagatgg cttccgaatt attcaagcca tttattatcc gaaagatgct tgagcgtgga
     3541 atagtcaaaa cagtaaaaac cgccaagaaa atcgtggatc ggaaagaccc ggtggtttgg
     3601 gatattttgg aaaacataat gaaaggccat ccggttttat taaatcgtgc accaactttg
     3661 catcgcctcg gaatccaggc atttcagcca aaacttatcg aagggaaagc tattcaatta
     3721 catccacttg tatgtacggc gttcaacgct gacttcgacg gagaccaaat ggcagtgcat
     3781 gttccgttag gaaatgccgc aattatcgaa gcgcagttac ttatgcttgc ttcccataat
     3841 attcttaatc cagcaaacgg cgctcccatt actgttcctt ctcaagatat ggtgctcgga
     3901 ctctactatt tgaccaaaga gcggaaaagc acacctgaga accctgttaa aggcgaagga
     3961 tataggtttt attcacctca ggaagttatc attgcttata atgaaggcaa agttgatctt
     4021 catgctattg ttagtgtaag attacctttt gaggaaaatg gtgaaactgt cttcaggctt
     4081 acagaaacta cggttggcag ggtattactt aatcaggtaa taccgccgga acatggttat
     4141 gttaatcaat tgcttaccaa aaaatcactt cgcgatatta taggaagtgt gctgaaaaag
     4201 actgatacag ccataacagc aaaattcctt gacgacataa aagatcttgg tttccgtatg
     4261 gcttttaagg gaggtttgtc attcaacctt gatgatgtta ttgtaccggt cgaaaaggaa
     4321 aagcttatag aagctgcaaa tgaggaggtt aacgaagttg ttaatagtta taataatgga
     4381 cttattacaa ctaacgaaag gtataatcag atagttgata tctggacaca taccaattcc
     4441 aagcttacca aagctctgat ggatcagata tcacaggatc agcaaggttt taacccggta
     4501 tatatgatgc tcgattcggg cgcccgtggt agtaaggagc agatcaggca gttgtcgggt
     4561 atgcgtggat taatggctaa accgcagaag tcaacagctt ccggccctga aattatagaa
     4621 aatccaatcc tctctaattt taaagagggg ctttctgtac ttgaatattt tatttcaacc
     4681 catggcgcca gaaaaggttt ggccgatact gctcttaaaa ctgcggatgc tggttatctt
     4741 acccgtcgtt tggttgatgt atcgcaagat gtaattataa ctgaagagga ttgcggaacg
     4801 ctcagaggtt tgacaactac agcgttgaaa aagaatgaag aagttgtaga agctctttac
     4861 gatcgtattt tagggcgtac cgcacttcat gatatttatc atcctcaaac caattcactt
     4921 atagttgagg caggagagga gattaacgaa gaaattgcac agaagattga agagtcgcct
     4981 attgagtcgg tggaaatccg ttcggtgttg acgtgcgaat caaaaatggg cgtttgtgca
     5041 aaatgctatg gtcgaaatct tgctaacggc cggatgctcc aaaaaggcga agctgttggt
     5101 gttattgcag ctcaatcaat cggtgagcct ggtacgcagc ttactctacg taccttccac
     5161 gttggtggtg tggccggagg aaatatttcg gcacaatcaa ccattgaagc taaatatgat
     5221 ggcgtattgg taattgaagg cttgagatct gttccttatg tatcgaaaaa cgataagaga
     5281 tataatgttg taataggacg ttcagccgaa atgagaattg ttgatcaaaa tactggtatt
     5341 aacttggctt ctcataatat tccatatgga tcgaatttat ttttcatgaa tggtgataaa
     5401 gtgaagaagg gtgaagtaat ttgttcatgg gatccgtata atgcggtaat agtttcggag
     5461 gttgcaggca aacttcgttt tgtaaacatt gaagaaggaa agacctttcg tgttgaatca
     5521 gatgaacaga ccggatattt tgaaaaagtg attatcgagg cacgtcaaaa gataaaaaat
     5581 ccatcaatag aagttgttga tgaaaacggt gaagtgctac gctcttatga tttgccggtg
     5641 ggttctcata tttcggtaga tgataatcag gaggtgaaag ccggtgatat acttatcaag
     5701 atacctcgtg ccattggtaa acgaagcgat atcactggag ggttaccgcg cgttactgaa
     5761 ttatttgaag ctcgtaaccc ttccgatcct gcaaaggttg ccgaaattga tggcgtggtt
     5821 tcgtttggaa aaaaacttaa gagaggtaat aaagagatca ttataacatc aaagagcggc
     5881 gaagaaaaga gatatcttgt ccctactaca atccaaatac ttgcgcaaga gaacgatttt
     5941 gtgaaagccg gaaccccatt gtccgatggc gctttaacac ctgccgatat attagcgatt
     6001 aaagggccaa tgaaggtaca agagtatatc gtgaacgaaa ttcaggaagt atatcgcttg
     6061 caaggcgtta agatcaacga taagcatttt gaggtaattg tacgtcagat gatgcgtaaa
     6121 gttgaagtag aagatcctgg cgatacaaaa tttctggaaa cagaattggt gaataaagtt
     6181 gattttcagg aagagaatga ctggattttt ggaagaaaag taattattga tccgggtgat
     6241 tcaactgtgt ttaaacctgg tcagattgta tcggcacgta aactgcggga tgaaaactca
     6301 tctttgaaac gacgtgataa acgtttggta gaagccagag atgcacaatc ggcaactgcc
     6361 cgtcaggtat tacaaggtat tactaaagca tcgttgcaaa caaatagttt catttctgcc
     6421 gcttcattcc aggaaacaac aaaggtgctt acaaatgcag caattaatgc aaaggctgat
     6481 gatctgattg gactgaaaga aaacgttatc gtaggacaca aaatccctgc aggcacaggg
     6541 ctaagagaat atgaggatat cctcgtagga tcgaaagaga gttacgatct attaattcag
     6601 cctaagcacg atgatatttt gtaaaaaata gttttcagga agaggccgct cataaaaggc
     6661 ttcttcctgc ttttattatc taaaaaaata aaatcatgga tcctaaacaa gcaagtcaga
     6721 ataaactgaa tatcgaactg ccggaagatg tagccgaagg cgtttactca aatcttgcca
     6781 tcattactca ttcaagatcg gaatttataa ttgacttcgt aaagatgcta ccgggaatac
     6841 ctaaagcaaa agtaaaatca aggattatcc tgactcctca acatgccaaa agacttttga
     6901 aagcgcttca agacaatgtg cgtaagtttg aatctatgca tggagagtta aaagataccg
     6961 atgccgatca aatgcctttt aatctttttg gtccaaatgc acaggcttaa aacgtttttt
     7021 agttttttaa ggaaaactat ttgttagata tgagttaatt tgctcaagtc ttcaaaaaaa
     7081 caatcctcct gtttataatg aaatccaaaa agagagagct gaacacggaa ttaaatattc
     7141 tcgcaagttc tccggttgta ccattgtaaa aaatttggag taaaatattg ccggaaacca
     7201 gattgggatt cagatttttt tcttaaaggc atattggaat aactgatttg tttgattgaa
     7261 aaagtaagag aatattttta atctcgttaa caatatttaa cagcaaaaaa ggataattaa
     7321 aaaaatagct tcgagttatt gttcttgttt taaatttata aaaaatgata gagataaata
     7381 tcagagaact gaacgaacgt attaatcagg aaagctcatt tattgacctt atcaatatcg
     7441 aaatgaataa ggtaattgtg ggtcagaagt atatgattga cagattgttg attggccttc
     7501 ttgctaatgg acacatcctt ttggaaggag ttcccggatt agctaagacg ttagcgataa
     7561 aatcgttggc taatgtgatt gacgccaaat tcaaccgaat ccagtttacc ccagatttac
     7621 tccctgccga tcttattgga acgcttatct atagccagaa aaccgaagaa tttattgttc
     7681 gtcaggggcc gatatttgct aattttattc ttgcagacga aattaaccgt tcgccagcta
     7741 aggtacaaag cgctttgttg gagtcaatgc aggagttgca agttactatc ggcgaacaga
     7801 cctataaatt acaggagcct tttttagtta tggctaccca aaacccgatt gagcaggaag
     7861 gaacatatcc attgcccgaa gcacaggtgg atcgttttat gatgaaggta gtgataacct
     7921 atcctgaaaa ggatgaagag aaattaatac taaggcaaaa cattagcgga actaatccta
     7981 ccacaaataa aattatctct cctaaagata tcctaaaggc cagagatgtg gtgagacagg
     8041 tttatcttga cgaaaaaatc gaaaattata tcaccgatat tgtttttgct acgcgattcc
     8101 cagctgatta taaactgaag aaatttgttg gaatgattaa ttacggagga tcgccgcgtg
     8161 cctcgataaa ccttgcactt gcttcaaaag cttatgcttt tataaaaaga cgtggatatg
     8221 ttatcccgga agatatcaga gcagtagcac acgacgtgtt gcgccatagg attggcctgt
     8281 cgtatgaggc cgaagccgaa aatataacaa ccgaagatgt aataaatgag attttaaata
     8341 tggttgaagt gccgtaaaat ttaaccgcat tataaaaatc aataatcatt ctctttgaaa
     8401 aaacatttcg gtatcatctt catttaatgc atgaacgcta cagaaatttt caagaaagta
     8461 agaaagattg aaatcaagac acgagggttg tctcatcaga tcttttcggg gcattatcat
     8521 agctctttca aaggaagggg tatggctttc agcgaggttc gtgaatacca atatggtgat
     8581 gatgttcgta atattgactg gaacgttact gcaaggtata atcgtccatt tgtgaagata
     8641 tacgaagagg aacgagaact tactgtcatg ctgcttattg atgtaagcgg atcaaacgaa
     8701 tttggcaccc gaatggctat gaaagaggat gtaattacag aaatagctgc agtcattgcc
     8761 ttttcagcca ttcataataa tgataaagta ggggtgatct tttttagtaa taaggttgaa
     8821 aaatttatcc ctcccaaaaa gggcgttacc catattttga ggattatacg cgaacttgta
     8881 gattttaaaa ctgaaaatca ggatactaac atcagcgatg cgttgaaatt tttaactaac
     8941 gccatcaaaa agaaatgtac cgcttttgta atctcagatt ttattgataa caaatttgct
     9001 gatgctattc agattgcaaa tagcaaacac gacgtcgtag caattagaat atacgatccg
     9061 cgcgagatgc aacttccgcc aatggggatg gcaaaattta aggatgcaga aacaggacga
     9121 aatatatggg tggatactac aaattcaaaa acccggaaac tttatgagga gtggtatcgc
     9181 aatagtgaaa aacagataaa aacgatattt actcgcagtg gagtggactc tgcccatatt
     9241 cgtaccgatg aggattacgt gaggcctttg ataaatatgt tcaaaaaacg ggaagcaagg
     9301 ttttaataaa gatgttaaaa gagagtaaaa tattgagaaa catattgcaa atatcatttt
     9361 taatagtttt aatactattt agtgttttat ccgtatcagc tcagaaggtg aggtttaaag
     9421 cttcgattga caccactaat atccttatcg gtgatcaggt gaaatacaaa cttgatttac
     9481 ttattccgca agactatctg tttgactggc caaggcttac agactctttg accaacaaga
     9541 ttgaaatcgt taatatgtcc gaaatagata cgataaaaag tgcggaagga gttttaaatg
     9601 tcagtcaggt ttttacaatt acttcgttcg ataccggttt ctaccttatt ccacccatag
     9661 aactcagata taaagagata agcgatacgg atgaggtatc aattgttgaa tcagagcctt
     9721 ttgttttaaa tgtcttttcc gttcctgttg atctttcgca gcctattaaa cctattaaag
     9781 gccctatggc tgccccagtt actttcaggg agttactacc ctggattttg attttattga
     9841 taattggaat tgcgctctat tttattatta cacgaaaaaa gaaaaagatt caacctgtct
     9901 tttttaagcc aaagcctaag ttgctgccgc atataattgc ttttaatgaa ttggaaaaat
     9961 tgaaaaaaga aaaattatgg caacagggtt tgactaaaga ttactatacc agattatcat
    10021 tgataatcag acaatacatc gaagatcggt tctctttgcc tgccatcgaa tcaacaacct
    10081 acgatactat gcaaatgatc agaaaattaa ctattgccag tgattcggtt ttacttttag
    10141 aaaatttgtt ggaacgttcc gatcttgtaa aatttgctaa ggctaaacct ctgccatcag
    10201 aacatgatca aagtatggat tttgccgaga gctttgttag acttactgct cagacagtag
    10261 aagttagaga aacccagaat gtagttgata aaagccatgg caatgatagt gaagtacaga
    10321 ttttagatca acatagtatg acgaacaacg aacctcccaa ataatttggc caatatgttc
    10381 aaggaatttc aatttgccaa tccggagtat ttttatattt tgctcatcat tccactgatg
    10441 atagcttggc attggtttaa aaacaatagg gcaaaagcag agattaaact ctctactttg
    10501 cttccctttg cttctacgaa aaagtcatat cgccaatatt tatatcactc tcttgtcgta
    10561 ctgcgaattg ctgctattgt tttattggta atagcgttgg cacgtcctca aaccatatcg
    10621 agtagtcatg atgtatcaat agaaggtata gatattgttt tagctcttga tatttcaggg
    10681 agtatgcttg ccgaggattt ttcaccaaat cgtattgaag ccgcaaaaga tttagctgtt
    10741 aagtttattg aaggccgtcc gggcgacagg atgggattgg tggtatttgc tggagaagct
    10801 tttacacagg tgccattgac aaccgaccat cgtgttctgt ctgatatgtt taaaaaagta
    10861 aaaacaggga tgatagaaga cggaactgct attggcgatg gacttgctac cgcagtggca
    10921 agattaacgg aaagcagtgc agttagtaag gttattattt tgcttaccga tggtgtaaat
    10981 aatatgggag tcgttgatcc gctttcggct gccgagattg ctaaactgaa aaaaataagg
    11041 gtttacagtg ttggcatcgg aactataggg acagcgccgt atcccgtaca aaccccattc
    11101 ggaattcgat atcaaaatat accggttgaa attgacgaag agttgctaaa aaatatttcg
    11161 gccgacacac atgggaaata ttttagggca accagtaata ccaggcttaa ggagatttat
    11221 gaagaaatag ataaactcga aaaatcgaaa attgatgtta ccgagtttta taaaaggaaa
    11281 gaggaatttt taccatttgc tgtgctggcg atgctgattt ttgtagtcga agtgcttttg
    11341 aaatatacct tttttagaac tacaccataa taatttttta tagctgatat gcagattttc
    11401 agatttgata atccttactt tttttgggcg cttttgttaa tcccactttt tattatcatt
    11461 tttagcttga tgatattttg gaaaaaaaac gcacttaaac gttttggtga atatcagctt
    11521 atcaggaggt taatgccgga tgtttcaaaa aacagactga tttttaaatt tatcctattg
    11581 atgcttgctt ttgtgatgat gatcattggg cttgctaatc cgcaaatagg ttcaaaactc
    11641 gaaaaggtag agcgcaaagg aattgacctt gtgatagcta ttgatgtttc ggagagtatg
    11701 cttgcacagg atattaagcc aaaccggctt cagcgagcac gtcaagctat ttcaagactg
    11761 gttgacgagt taaaaaatga tcgtattggg attgtggtct tcgcaggaaa ggcatataca
    11821 caacttccaa ttactaccga ctattctgcc gtaaggcttt ttatctcgaa tatcagcgcc
    11881 gaaagtattc cggtacaagg tacggcaatt gccgaagcta taaaccttgc agttgatctt
    11941 tttgatgaga ctgaaactga caaagctatt attgtaatat ccgatgggga agatcatgaa
    12001 ggagatgtaa tcgaatcggc taaaaatgca gtaaagaaaa acattcggat atacacaatt
    12061 ggaatgggtt tgcccgatgg ggcgccaata cctgttattg atagatacgg cgtgcagcaa
    12121 gggtttaaac gtgacagaaa taataatacg gtcatttcaa agctggatga aaacatgttg
    12181 aaagagattg ccgaagccgg aatgggtaat tacgtaagag caaataatac gcaagtaggg
    12241 ttgaggataa tttttgatga aatatcaaaa cttgaaaaaa cggagatcga atcccgaatg
    12301 tttacagatt atgaaaatcg gtttcaatat ttcttagcgc ctgctctact gttactactg
    12361 attgaatttt tgatatttga acgaagaagt agcagatttc gcaaggttaa tatttttggt
    12421 gaaaataaac tttaattctt gaggttatta ttcttatgat ttttgaattt aataaaacgt
    12481 ttagtaaaac tgccattctc cttttagtcc tctttttgac ggacataagt ttgatggctc
    12541 agggcgaaaa ccgttttatt cggagtggga ataaagcttt cgaaaaagga gagtttaata
    12601 aagccgaaat tgactataaa aaagcccttg aaaaaaactc taattccaat gttggccaat
    12661 ttaatcttgg tagcgctata catgaacaaa aaaattatga tgaatcggca ttgatatttg
    12721 acaacctgac tcacaaaaac ctgactcctg accagcgtgc gaaaagtttt tataacttag
    12781 gaaattcgtt ggttaacttg caacaatacg aacaagcggt ggaggcttat aaaaactcat
    12841 taaggattaa tcctggcgac gaggatacaa aatacaacct gctttatgcc cagcaaatgc
    12901 tcaggcaaca gcagcaaaat gaacaggatc aggacaatca acaagatcag aatcaggatc
    12961 agaatcagca aaatcaagaa cagaacgatc aacaaaatca ggatcagaat caggatcaga
    13021 atcagcaaaa ccagcaaaat gatcaacaag gtcaaccaca agactctaaa gaagatcggg
    13081 cacagagtga gccggtgcaa atttctattg aggatgctga acgaatgtta caggccttgc
    13141 aagaccgaga gcgcaagact ttggaaaaga ttaaaaagga agagctgaaa aatgcgaaaa
    13201 gggtaaaaac cgaaaaagat tggtagttag ataggataaa caccagcgtt gaaagataga
    13261 tatgaaaaat catttgataa aaacaggtta ttcgattttg tttttaatgg ctctgtggtt
    13321 gctgtttttt aatgggaagt taatggccca ggagccacaa ttcgttgcgt cagctaaagc
    13381 agtggttgtt gtaggcgaga ggtttcaatt gcaatatcgg tttactgccg aaggtgtcaa
    13441 tttcaaaggt ccaattatta aggactttaa cactttgagc ggcccatcac cctctacaag
    13501 ctccagcgta caaatcatta atggccaggt atcacgagaa gtatcctata cttttacata
    13561 tattttggag gcttttaaag aaggtaaatt tgttattcct ccggcaacta tcacctacaa
    13621 caataaacaa tactcctcta atggacttac aatatcggta aatgcaggca gacaagcccc
    13681 gtcatccggc cagagcccac aacaaggcgg gcaacagcaa cagtcaacgg ctgacattga
    13741 tgtattcttg aaagctgaaa taagcaacgt aaccccttac attggtgaac agattattat
    13801 atcctacaag ctttatttca acaatcagat tgccggccat gacggatttc aaaaactctc
    13861 ttcttttccc ggattttggg taaaagacct tttggcaaat cagcgtgata tccctacaaa
    13921 aaaggaggtt tataaaaaca aacaatacaa tgtggccgaa gtgcgtagat ttgctctctt
    13981 tccgcaaaga gccggcgaaa ttgaaattat accaaaagaa actgatctta cggttagagt
    14041 acaaacggct tcaagacgaa gatcttcgga ctcctttttt gatagttttt tcaacgatcc
    14101 attctttaat agccgatata gcgatgttaa gaagaaaatc gtttccaacg gatttactat
    14161 caatgtaaag ccaactccaa ctaaagataa acctgataat tttagcggag cagttggaac
    14221 gtttaccatg aaatcgcaaa ttgataatac tgaagtaaag gccaacgaac cgattactat
    14281 aaaacttact gtacaaggag cagggaatat tgaacttttc gacctgccaa atattgtctt
    14341 tccaactgat tttgaggttt ttgatcctga aatgaaaagc gatattaaag tcgccaatac
    14401 cggtgtttca ggtacgcgaa cttttgaata cctcatcatt ccacgtaatc ccggtaactt
    14461 taaaatcaag cctatcgagt tttcatcctt taacccggct caaggagctt ataatgtgca
    14521 acgcagcccc gaatatagta taactgtcca gcgaggcgat ggctcctcat cagtgataac
    14581 ctatggcggt acagtgcgcc aagagggtat tcaaataatt ggctcagaca ttagacatat
    14641 aaaactatct ccgtataatt tttcaaatat cggagtctat tttttccgtt catatctcta
    14701 ttatttgctg cttatcatcc ctttcctttt aacgatagct attttaatta tttggagaaa
    14761 agagatgaaa aagcgtgctg atgtcgagtt aatgaggaat cttaaatcaa acaaaatagc
    14821 gcgtaaacgc ttgaagcaag cgaatatatt tatgaaagcc ggaaatgaga acgaatttta
    14881 caatgaaatt gcccgtgctt catggggcta tcttagcgat aagcttaata tccctgtgtc
    14941 agagctttcg atggataatg tcaattcaag attattggct aaaggcttga aagaggagac
    15001 aatccagcag ttcatcgaaa cccttaaaca aacggagttc gctcgttttg ctcccggcac
    15061 taaagacgaa aatatggaac gcctctattc cggagcgctt gaaataatta ctaagattga
    15121 aaaagaatta aaatagacgc caaatcatga aaaagagtct aataattgtt ttcctggttt
    15181 ttggttctca actatttgct tcagataatc tcttgttaat agagcaagcc aataaatatt
    15241 atgacgagag tgaatattct ttggcagtcg aaacgtatga aaccataatc aacaatggat
    15301 ttgagtcgga taagctgtac tacaaccttg gcaacgctta ttttaaactc aacaaccttc
    15361 caatggctat attttattac gaaaaagcca aaaagcttaa tccttatgat gctgacatac
    15421 tttttaatct tgagattgcc aacggcagga tcattgataa aatagaacct gtccctcagt
    15481 tttttctatt caaatggtgg gatttgttgg tcaaatcacg tacgatggag cagtgggcga
    15541 gagttagtat tttctctttt atcatatttc tggtaatggc gcttgtgttt ttattagcaa
    15601 ggttggtttg gttacgaaaa actggttttt gggtaggagt tttatcatta gctttattta
    15661 tcctgtcgtt tatattagcc aatcaacaat ataattcttt taatcatcgc tccgaagcta
    15721 ttatttttac tccaacagta acggttaaaa gctcacccag agataatagt accgatattt
    15781 ttgttatcca tgaaggttta aaagtccagt tgactaatca ggtaggcgat tggtatgaga
    15841 taaaaattgc cgacggcagt aaaggctgga taaaagagaa tgatattaag ctgatataaa
    15901 aaatgcctca tgtgaggcat aaattgaaaa agattatttt taaaatccaa gcttcgaatt
    15961 taatccagcg ggtaaacctt ttttatttac tacgataaag ccagtgttca attttaaaaa
    16021 tgcagatgaa agatgaagtg tctgatcacc agcttcacat acgttctttg ctattaaata
    16081 ttcagttcca tttttgtcct tgcttatccc cgaaatcaac agataatttt gttccgtttt
    16141 agccgaagtt tcaagaacag cttgacgttc tgtgtttgtt attgtttttt cgggtagcgg
    16201 tttaaagctg ttttcgtcag tctcatcgcc ctgtgtaagg tcggcaattt ttccatcagg
    16261 gacaatggct actttttccg catcatagat catttcatcg ggtaggctgc cataccatat
    16321 tgtgcaatat ccattattta cagcagactg aatcacactt acaagaatat ctggattaac
    16381 attgtaagcc tgacttttat tccagttatt ttttgcagta agggtgattt ttgtatccat
    16441 tgccgatttg tcatctgatg ttagagtaac aaaatcggat gcttttaaac ctgattgggc
    16501 agcaaatgat tttggagtat agtctttgcc ttcataattg aaattgatac gggtttcgcc
    16561 taaatagcgg tatagtgagg cgttgaaagt gtttttccag ttttcggtaa agttaccgcc
    16621 ctctttttcc atcaccatgt gtagagtggc tcttattata gcatccattt cacctgatga
    16681 ggctttttca ggtttatctt ccgacttcat gtacattgat tctggagcca tgccatactc
    16741 ttgcataaga gtaaaaacat cttgtggtaa gcccttttcg tcaacataaa tagcgccctt
    16801 ggaactgaca tgagcatttg cttttaactt gtacaaatcg gtgataaatg tcatttcaga
    16861 aaggtcaatt tcctgatttc tatttttaat ccattcagct tcaagtaatg ctgtgccggc
    16921 attggcccag cacgagcctg ttcgttgaat atctttggaa ggagttgtct tgatctgttt
    16981 taccgtttta atatcgtaaa acgtcttttg tgccgaaacg ctacccacta caactaaaaa
    17041 agataatact gtaatggcta atcttgaaaa atttttcatt ggaatttatg attttggtta
    17101 ataattgttg ttttgtttaa attgattgca aaggtagtac ttaaagcaga tgttgtacca
    17161 aatattgtgc aagaatgaaa aatgataaaa ttgccttatt caaaattgac tataatgatt
    17221 atttttgctc ttttaggatg aaagtaactt accgataatc ttaattttta gtgttttgct
    17281 atttaaaatc aatttatatt gactaataaa tgattttggt ttgaattttt ttttcaattt
    17341 tgctaaatca atttaaaata aaaaaaacgt actaaaatat ttaatatcaa tatattgaga
    17401 gtgtttctaa ggagtaaata ctatctcagg acttgtttaa agatagaggt tcacaagtgt
    17461 ttgatactaa tgctacctga tgattttaat aattttattc aacaaaaatc aacgggttaa
    17521 atgaaaagaa gtattttagt gccttgtttt gaaaaaataa aatagaaaaa atataataat
    17581 caaattttac gaatatgaaa cgtaaacttt tacaattgag ctttttagtg ttgatttttg
    17641 cgatggctgg gctctttgtt gcatttgctt acaccggcaa tcagcagcca ggttcttcga
    17701 aaaatgctaa aactgatttt gaacgagcca ctgaatacct gcagcaaatg cgaaacaatc
    17761 agattaccgg tgaaataaat ccgtttgatg tgctcaatgc caggcagcaa gcagaaaaga
    17821 tgtattttaa atctggaaaa gcatttgatc ttgattggct tgaacttggc cctgacaacg
    17881 cacccggtcg tgtcagagct ttaatttttg acaatcgcga tgaaactgca aagaccttga
    17941 tcgctgcggg aataaccgga gggctctgga aaactacaaa cttgggagca acatggaaaa
    18001 agataaatgt tgcgaatcaa aatctttatg tatcatcatt ggctcaaagt gccgacggca
    18061 ctatctatgt cggaacgggc gaatatttct gcacaacaaa cgaaacttat tttggaggac
    18121 ttgttggcac tggaatatac aaatcaacca actctgatga atttacattg attgaagaga
    18181 ctaaaccttc cataactgcc ctaacagata ccgttgattg ggcttatatc aataaattag
    18241 ttgtaagcaa aaactcaggt agaatatttg ctgcaaccaa taccggtttg tggtattctg
    18301 ataatggcga ttcatggaca aaggttactc aatattacca tgacacacta acttataatg
    18361 ttaccatttc aattgattcc actgtaaaat gcgatagttt cattgttgag ggtaacaata
    18421 ttatcatgaa taatcctcag atgaacaaag ctgttgtgga tacggtcagt tatgaaaaga
    18481 caaaacagac gccgatacgt actgtcatga gttttgcaaa aattaattgc acggatgtcg
    18541 aggtaggagg cgatggtacg gtagcagcaa cttttggaga tatggtgttt acagccaatt
    18601 ctaatgattt gatttttaca aacagatcgg gaaccccttc caatccaaac ttgattacca
    18661 gagaggatag agattataca acacggctga tagctgtaga tactcttaat aatactgctg
    18721 atcgcactgt tacttttaat caaatcacca atttcgtagc agatcctgct ttaggccgcc
    18781 ccagcccgtt atccaataat ccaagtcgcg ctaatgtagc ttttgcacca aatgaccctt
    18841 cgggtgatat cttgtatgcc gttagcacta tggccggata tttatacaat gtttatctct
    18901 cagaagataa aggagagacg tggagaacaa ttttcccagg cggctccaat acattaaagc
    18961 ccttcaatgg tacggcttgc tataacaata ctttcgaagt ctttcctgat gataattata
    19021 aggtgttact tggaggccaa actatgtgga tgggcacaca ggttggtaac gggggctttt
    19081 tcgattgggg attaggccct attacagcat ctttgccctc aggtcatcat gtttatgcat
    19141 ttcagcccgg tactaataac aaattagtag tagcaactaa tcttgggatt tattatggta
    19201 ctttaggggt agcttataac caatttactg aaattagtcg caatttaacg ataacacaat
    19261 gttatacagt ggcaaccgga ggattgaaac gaaaggtgct cacaggaacc cagggcgatg
    19321 gaacttggta tattagcgga ttaggtaata caccgcagta tggcgaaaag attgacggtg
    19381 gacatggagg gtcatgcgtg atttctatta tcaatcctaa tgcatttatt tttagttcaa
    19441 gtaccggcac aatacaacgc tctgacgatg aaggtcaaaa tgcttcgatg aacttctcgg
    19501 cgccaagtac aacattgttt attacaccta ttgctttttg ggaagatttt aaatctgaaa
    19561 actccaggga ttccattact ttcaaggcaa ggcaagatta taaagccgga gatgtaatcc
    19621 tttgtcgaag caaaaatcca ggattcggac ttgatgccgg ctatcctttt gattatgttt
    19681 tagaaacaga tttggctgaa ggtgattcga ttcgaataaa agatattatt cagtcaaaat
    19741 tattcgttgc caatcaaaac actgtctatt taacaaaaga tggagtgaag ttcggcaaag
    19801 agagcgagtg gtggcaaata gcttcgcttg taacagctaa tggacagcct acagctattg
    19861 cttattcaaa agatgcaaat tatcttttta tcggaacaaa agctggattt atttacagaa
    19921 tatctaatat agcgcacgct tatgattatg aaagagctga tatcagaagc ccttattgta
    19981 ttatttcaac agatgttatt cagatcccgg agtttgaaaa tagatttatc acttctattt
    20041 ccgttgatcc taacaacagc aataatatta tcgtaacatt aggaaattat gggaatgaag
    20101 gttatgttta tcgctctacc aatgcactgg catcaccatg gacaggtatt acctttgttg
    20161 atattacagg taatatgcca aaaatgccag tttactcttc gcttatcgag atgaaggact
    20221 ccaataaagc tataatcggt acagaatatg gcatttatac taccgatgag attggtaagg
    20281 cagatgttac atggactgcg gaaaatcaag gaatagggtt gatccccgtt ttccagataa
    20341 agcaacagaa gatttataag gacgagtatg tcgtgccaag cgaagatccg tctcagcctc
    20401 ctttggttta tccacgagtg gataattatg gatcaatata catagccacc tttggccggg
    20461 gtacttatag agctgatagg tttatggttg gtattgatga tattattcca acgagcaaca
    20521 agaatattga aacgctaaat atttatccta atcctgttga tcgtaacacc acgttatctt
    20581 tcaaccttaa caggaatagt aacattcaga tttcgattta taatttaagc ggtctccttg
    20641 ttaatacact taatgcaggc acatattatg ctggttttaa ttctgtaaca cttgattgct
    20701 cacgtctgac aagtggtgca tatcttatta aaattgatgc agagaatgaa attatgactg
    20761 caaaaatgat tgtacgttaa ttatttaagt aatttaaagc taaataaata atacaatgaa
    20821 aaaatatttt ttatccacaa tattgataac tgcagctacc ctaattatgg ctcaggtgaa
    20881 agttgaacct cctgttttaa ttgcaccagc aaatgctgca actaagcaaa tgcctgattt
    20941 aatacttgat tggaaggctg taattggcgc attaaactac gagcttcaat tgagtgaaga
    21001 tgaggctttt acaactatcg tactggattc tattactgat ctaactgccg taaaatcaag
    21061 tatgttaaag ttttgtcagg aatattattg gagagttcgg gctctttacc ccggtaatat
    21121 ccactccgat tggtcaaccg tatggatgtt tacaatattt tccaattttg atttagtaaa
    21181 accaaatgat aatgctgaaa atgtgccccc tgacaccgag cttaagtgga aagataaggt
    21241 aggccctctt tcgataaccg gtgtttccta tttcgaagtc caaattgata gtgttgatac
    21301 ttttgattca cctgagtttc atgagttttt cgttggtggg aatgttttta aaaaggatat
    21361 gtccaatctt ttgtttggta caaaatatta ctggcgagca cgtgcaggcc atgacaaaag
    21421 tgtttcggag tggagcgacg taaggagttt tacaactgtt ggcaaagtaa ctctccaaag
    21481 gcctaataat aattccgttg ataatgatct gaatgtaaac ttaaggtggg gagaaattac
    21541 tggtatttta aaatatgagt atcagataga tgttgatggt tcctttagtg cgccaatggt
    21601 cgaacttact gagaatatca ttgaaccggc ttcgaatctg atgtatggaa cacaatatta
    21661 ttggcgcgtc agagcacgtc attcacgaga tactactaac tggtcagatg tttggaattt
    21721 tacaacattt aatttcccta ctttgacttc ccctgctgat aatgatacag gaattatttt
    21781 gcgtccacaa ttgaaatggg atcagattaa aggttcgatc aaatatgaga tcaatcttag
    21841 tttagatgag gcaacactgt ccgatcacaa atactacaaa gatgctagtg atgccgaatt
    21901 accccattat aacgttgcgc ccgatttaga gccattaaca aaatactttt ggcgagtacg
    21961 tgcttttagt gaaattgata caagtgattt tagttccgtt tggtcattta ccacatatac
    22021 accggtgggt atcaacgagt attttgcaag ttcaaatgtt aacttatacc caaatcctgt
    22081 tcaggatgct attaatattg agatcacttg caataagttt acaaatgttg atttagtaat
    22141 atacgatttg ttaggacagg ctatgttgaa ggataacctg aatttaaaca gcggccataa
    22201 tcaagttcag attgatgcca ggactttact aaatggtatt tatatggtaa atattatgta
    22261 tggcgatcag gtcattacta aaaaacttgt tgttagcaag taaatagacc ttttcaaata
    22321 ttaaaaaagc cgctttactc tgaggaaagc ggcttttttt gagctttttc caaataatgg
    22381 cgtttagctg cctaatgttg ctaccattac tgcttttatt gtatgtagcc tgttttcagc
    22441 ttgatcaaag actattgagc gatctgattc aaagacctcg tctgatacct ccatttcagg
    22501 tattccgaat tttttataaa tcatttcgcc tatttctgtc tcacgattat gaaatgacgg
    22561 taaacagtgc ataaatttaa catcaggatt tccggttttt ttaatcaaat ccatgtttac
    22621 ctgataaggt atcatgttgt taatcctact gttccatgct tcttccggtt cgcccataga
    22681 gacccaaacg tcggtgtaaa gaaaatcaac acctttcaca ccaatttcaa cattatcggt
    22741 caatgtgatc ttagcgccgg ttgttttagc tatttcacta cattgttcaa caagttcttt
    22801 ggctggcaag agttctttgg gagctactgc tctaaaatcc atgcccattt tggcggcacc
    22861 aaccatcagg gagttcccca tgttattacg tgcatcgccc agatatgcaa atgcaagctg
    22921 atttaatggc ttaatagagt gttcacgcat agttaagaaa tcggcaagta tctgcgttgg
    22981 atgaaatgag tcggtcaggc cattccatac cggtactccg gcgtattttc caagctcttc
    23041 aacaatttcc tgcccaaaac cgcggtattc aataccatcg tacattcggc ccaataccct
    23101 ggcagtatct ttcattgatt ctttttttcc agcttgcgaa cctgacggcc ccaaataagt
    23161 aactctggcg ccttgatcga aagctgctac ctcaaaagca caacgagtac gagtggaatc
    23221 tttggcaaaa agtaagacta tgttcttgcc ttttaaacga ggttgttctg ttccggcata
    23281 tttcgccttt tttaattcgg atgaaagatc aagtaaaaac atgatctctt gaggggtgaa
    23341 atcgagtaat ttcagaaaat ttctgtttct taaattaaaa gccataagat taataattat
    23401 attttaatga aaaaaaatgt ttacaacaaa gtcattgtat ttttaatgaa aaaaactaca
    23461 accacatagt taagtatctc ttataaaaca ttttgtgttt acaccatcac tatccttgta
    23521 ccaccattat ccactcccaa gcttgtggtt ttagtaatta tagcatcttt tccactatgt
    23581 tctacaaagt aaattgcagc tctgattttt ggcgccatac tcccttcagc aaaatgtccc
    23641 tcttcaaggt aacgttttgc ttcagcgata gtaatctggt caagtgtttt ttcctgtgga
    23701 gtattgaaat tcagacatac tttcggaaca tcagtgagga caaaaagttt atcagcatta
    23761 atttgagcag ctaacagggc agaggcgagg tctttgtcaa taacagcatc aataccttta
    23821 agtttgtttt ctgagacata gtaaacgggt attccaccac caccaacagc tattactatc
    23881 gaaccttgcc gggcaatatt ttcgatggtt tttttattat ttataactaa tggttttggt
    23941 gaagccacca cttttcgcca tcccctgccg cgtggatcct cggcaaaaac agcgccgctc
    24001 tcttttataa tcctgtctgc ctcttctttg gtataataag gaccaattgg cttggtcggg
    24061 ttattaaaag caggatcgtc cttgtttacc aaaacctgtg ttataatagt gatgacatca
    24121 cggtcgagat cggtttgcaa gagtacgttt ttcaattgtt gctccaatac ataaccgata
    24181 aaaccctgtg aataggctac acatatacta agaggcattt cgggcaaacc atacagtttg
    24241 ttacctgcta tattgttaag catcagattt ccaacctgag ggccattacc atgagtgagg
    24301 acaaggttgt aatttttctt ttttatcagg gttaccaatt tttcacaggt ttcgtaagcg
    24361 ttttgttcct gttcatcaat ggtgccaacc tgcccgccct gaagaagtgc attgccgcca
    24421 aaagctatta ctgctaactg attcatataa atgatatatg gtttaaaaat ccgtgcaaaa
    24481 atatgtaaaa ttgggtgatg gcaaaggata ccagattaca cattctaacg taaagggttt
    24541 tttaatccaa aatgattaat aaaaagctct tttaggctaa cctgttgatt ttttgaggca
    24601 aattacgatt cagtgttgga atcttcatcg ctgaattgat gctttgggta attaatggta
    24661 attaaaatgt agtttcgaac gatctgtttt tattcttaat tgtgtcgaaa aaaaatgagt
    24721 agggcctaat taatactcca gcgatagccc aaacttttgc tttaaatcag caactatcgg
    24781 atttttggcc agcatgtgtt ggtaaatttc atccggttta tatggtcttg tttgtacatt
    24841 agtagtgttc aacaaaatct taagctgtat aaaaggatta tcaagctcgt tcctcaaatg
    24901 agcaacgagt tcgtttttct ttctattaag ctcatgttct tgaagcgcat tgtgaacctc
    24961 aaaggtgatt atgaagtttt cttcaatttc tggatttgtg gaaacgagtg tgctgtaaag
    25021 gttaggattt gcatccgata ttttggtggc aaagtcattc caaacttgta gaagttgatt
    25081 agtttggata gttttagcgt ttaaaaattc ttctgattca aaatcaaaat tttctgcttc
    25141 ttcattctct tgaccgtcat ctttttctat tacttcatct tttattgaga tggtggttgg
    25201 tatatatggc agtttcaaag tttctgaaac ctgaggaggt tttgtttttg ttatttcttt
    25261 tttctctttg gtaaccgatt gatagtatgc tggtttttgt gttgaggctg tagttggtag
    25321 tgttttttgt ggttttggtg ttgatgggat aactattggc tcatttttat cttcgacttt
    25381 gtccgttgat aaaacagctt ttttgtcggc acttagagtc tcatttattg aagcaagttg
    25441 catcaacgct atttctatgt gaaggcgttt gctgctactg gttttgtagt tgaaatctgt
    25501 tttttgaata atatccagcc cattgatgag aaaagtggga gtgcatcggg ccgattgctc
    25561 taaatagaga tcttttatct tttctcccac ttcaagcaaa tcaacggttt tgtcgtcagt
    25621 acacaccaaa agatcacgaa aatgcgctcc aagtcctgaa atgaaatgtt gaccatcaaa
    25681 acccgattca attaccttgt tgaaaagtaa aagagtgtca tagatgttat tagaaagtaa
    25741 aaaatcagtg attttaaaat aataatcgta atcgagtaca ttaagatgat taactacaag
    25801 tttgtagtta agctcttttc ctgtaaaact caccaactga tcaaagatcg aaagggcatc
    25861 gcgcattgcg ccgtcagctt tgtgagctat tacttgaagc gcttcaggct cggctattac
    25921 gccctcttcg ttggcgacgt atgcaaggta tttggcaata tcgcgtactg ttatacgctt
    25981 aaagtcgtaa atctgacaac gggaaagaat agtgggaata attttgtgtt tttcagtcgt
    26041 tgcaagaatg aatttagcat agaggggcgg ctcttctaac gtttttaaaa aagcattgaa
    26101 ggccatttgc gacaacatgt gtacctcgtc aatgatatat actttgtatt gaactgattg
    26161 agggggtatg cgtacttgat ctactaaatt acgaatatcg ttaacagagc tgtttgatgc
    26221 agcatccagc tcaaaaatat taaacgatgc actacgatta aaagaaagac atgattcgca
    26281 tgcattacat ggttcaatat tttcatcaag atttagacag tttattgtct ttgcgaaaat
    26341 acgagcgcaa gtggttttac ctacacccct tggaccggta aaaagaaatg cctgtgcaag
    26401 ggttttggta cggatagcat ttttaagagt cgtagttata tgatcctgac ccacaacggt
    26461 gtcgaaagtg gccggacgat atttgcgggc agaaacgata aaatcatcca tagttttcct
    26521 ttaaatgatt tacaaaaata tgattttctt aggcatggaa taatgataaa ataatactga
    26581 aactactgga attattacat ttgcctttca aaatgtgcga tatgatattt atttatactc
    26641 caaaaataac taaaagggtt gagtatattt tcaaactttt tttcaagcaa ctgcttaata
    26701 ttgagtatga gattacattg aaaccggaaa tttttaaaca atacaatggc gtcaaaatca
    26761 attactcaaa tcagcgcttt gaagattcat tgaatttcca acctgtagat ttacttttca
    26821 aaaccggtat tgatagtcag gaacttaaaa ccatagttta caaaggtcaa aaagtttttt
    26881 ttcctgttta cgatcctaag tcaaatttgc cttttgatcc ttttgctgct gctttttacc
    26941 tcgtttcgcg ctacgaagag tatcttcctt ataaaaaaga tcggttcggc aggtttgatg
    27001 cacctgagag cttttctttt caacaaaata tccttgataa accacttgtc aatatttggg
    27061 cttattggat tcgtaatttg ctgttggaaa aattcccaga tctgaagttc cgaaatagaa
    27121 catttcaatt tttgcctact tacgatattg atagcgcgta tgcttataaa aacaaaggtt
    27181 ttgtaaggat tgccgccaac ttcgctcgcg atctgattaa tggcaggttg agcgatatga
    27241 aagaacgatt cagggtgatt atcggtaaac aagccgatcc gttcgataca tttgactttc
    27301 agtttagctt gcagagagaa tatgatctgc aaccacttta ttttatttta atagctaatt
    27361 atggggagta cgataaaaat ttacctgtta acaatttgaa atttcaacag cttataaaat
    27421 ccctttctga ttacgccgaa gtaggcattc atccctcttt tggatcaagc acaagttata
    27481 aactcctgac cagcgagatc gagcggcttt ccagaatatt aaaccgggag attgttatca
    27541 gtagacaaca ttttcttagg ctcgattttc ctatcactta tcgtaatctt atccaggccg
    27601 atattacaga tgattataca atgggatatg cctcagcgcc tggctttaga gctggtatct
    27661 gcgatgcttt taatttttac gacctcgata tggaagtaga aacttctcta cggatacatc
    27721 catttcaggt aatggatggt accttacggg attatatgca gacagatgtt gacggggcaa
    27781 tagaaataat ccgaaagctg atagatgaag taaaggctgt ggatggcact tttagcagtt
    27841 tgtggcataa cgaatcgtta agtaataaga agcgatggga gggatggcgc actctttatg
    27901 tggatatgat ccggcatgcg ttaatctaac gattatattt cgccattatc ggcaaatgag
    27961 aaataacttt ccattcctat aattatatga tcaagcattc tgatttcgag taaatctcca
    28021 ccttgtttta atctattagt cagcctgcgg tcctgctcac ttggttttgt gttacctgat
    28081 ggatggttat gggcaaggat gatagaggtg gcgttgtttt ctattgcatg cttaaaaacc
    28141 ttttttgggt caactatagt accggtaaca ccaccttcgc tgatattcac cgttttgatg
    28201 atcttcattg cttgattaag tagtattatg aaaaagtttt cgtaatttgg atcgctgatg
    28261 tgcatttgca aaagttcaaa ggcatcccga ctactcttta tcgtctttcg atcaatgacc
    28321 tcagcgccac ggcgcctatt gcctaattca agagccgata ctattgtaat agctttggct
    28381 tgaccaattc ctttgaattt tttaagatca tttacgttga gtttcgaaag ctcaatcaaa
    28441 ttgttgttta cgctttttag tattcgtttc gacagatcaa cggctgattc atctttagta
    28501 cctgaagcga tgaggatagc tatcagttcg gcatcgctca atgaagcttt tcctttaagc
    28561 atcatttttt cacgtggccg gtcatcttcc gaccagtttt ttatggattg tcgtcgttta
    28621 tcttctttca taacttatgg gtttaaacaa aaaaagcctc taatgcaata acaccagagg
    28681 ccggtcaata taattgagtt gattgattta ctcggtagca acgagttttt tcgaaagctt
    28741 cgatttcaga ttggcggctt tgtttttatg aatcactttg cgttttgcat tcttatcaat
    28801 cagcgaaact aattttggat actgatctct ggctgtttca atatcagtta acgacttgaa
    28861 ttttctaatt gcattgcgca ttgtcttcgc gtgataccgg ttgcgcatct gtttcatctt
    28921 gtttgccctg attcttttta aggctgattt atgatttgcc atctatttaa ttaatttaat
    28981 ttcagtgtct tgtagaaaaa ttatcgtttt tatctcataa aaattatggg ctgcaaaatt
    29041 acaatagttt tttttctaaa caagagaatt gatagataaa aactatttta gtaaataact
    29101 ctcaagaatt ttatccagtt ctgccggtgt aaagagttta tttaccacta ttccgttgcg
    29161 gtcaatcaga aaagtgccgg gaagattttt aactccaaaa aatcttacat cacgatcttt
    29221 ccaactgtca attacgtaaa cattgttttt ccatggatat ttcttttcgt tgagtgcttt
    29281 aatccatgtt tcgcgctctt tatccagacc aattgcgtaa atgtcgtaac ccttaccatg
    29341 aataaaattt ttatcgtgat acttattgta tatttcacga tgtttttcgt tgtccaccat
    29401 gcaatgatgg caaagagagt tccacagcat caccattacc attttgccat taaggctgct
    29461 caactttata agagcgctgt cgggatcgta atttgcaaag tctccaagat cgttgcctac
    29521 ttcgttacct acaacaatct cttccgttga tgtggtttga tttggatttg agtcttgatt
    29581 ttttacaggc tgagtaattg ttgtttgagg agcggtttta gaagattttc ccccatttcc
    29641 aggcggcggc aattccggag gcgtttgatg tatgactgat gagcttggtt gatcttgtac
    29701 aagcgtttgg tttgtaactt gctcacctga ttcagtggtg ttggctggct ggtttttgct
    29761 ttgattcgag caggatgcaa aaacaataat aacaagccct aaaataacga aaaataaatc
    29821 ttttttcatg gtttttctta attcgatagc attaaaaatt tttgcaaaat tagctttttc
    29881 tgtaattagt aaatcattga agccataaac ttttgcatag ctttaataag attgataagc
    29941 agatcatatt tagttgtaat ttgcttattc gagtaaaggt ttaatgtaga aaaatgttca
    30001 accggacaga tcttttaaaa ggaaaaatcg tgttttttta tctttaagag ataataatcc
    30061 tttgtaattt agatattttt gcaatgtatt gactgatgtt tttgttcttt ttgcaagctg
    30121 ccaatttgag aaaattattt ttaaaaacta ttgaaagagt atttgactct atttaaacgt
    30181 atgtaggata tgaattttat tgaaaataag cttacggcgc tttatcggcg atggagtgga
    30241 atggaacccg aaacgattac tcaattgcca caatctggct cttatcggca atattttaga
    30301 atgaccggaa aggataaaac atttattgga gcatattatg atcaagtggc agagaatatg
    30361 gcttttatct ctttttcaag gcattttgcc aaaatgaacc ttaacgtgcc tgaaattttt
    30421 gctgttgatg atgactcggt atgctatctc cttgaagatt tgggagatat agtattattt
    30481 gattatttac agaaaatcag atctgcagaa ggatttggag agcattcttt atctatttat
    30541 aaaaaaattc ttgacgaact tgtaaaattt caaatggttg ccgggaaatc gctcgattac
    30601 tcagtctgtt atcctgcctc ttactttgat gagcaggctt atatgtggga tttgaattat
    30661 ttcaagtata actttttgaa gctggcaaac ataccttaca atgaattttc gttggagaat
    30721 gatttcaaga tgttgacacg tttacttttg accgctgatc atcattattt tgtttatcgt
    30781 gattttcaga cacgaaacat tttgtatcgt aatgacgaac tttactttat tgattaccag
    30841 ggtgggcgaa agggtacact tcattatgat gtagcttcat tgctttttca ggcccgagcc
    30901 gaaataccgt ttggagatcg tgagctatta cttgattact atatttcgca agctggtaaa
    30961 attgagccgg gcatcttgag aaactttaaa gatcattatt atctttttgt gctgataaga
    31021 gtgcttcaaa ccttaggagc ttatggtttt agaggtttac atgaaaaaaa acaatatttc
    31081 atttcaagta ttcctttggc tttaattaac gtaaagtggc tgcttgaaaa tgaaaaaatt
    31141 cctgaaactt tgcctgagat cagaaaagtt cttgaggcta ctcttgaaag caattcattg
    31201 gatgcgttgg taaagccacg actaatttta aagattaata gtttttcgta taaacgcggt
    31261 tttccagccg acacctcggg gcatggcggt ggttttgtat ttgattgtag ggcgcttcca
    31321 aacccgggac gttaccctga atatatccaa atgacaggat tagatgagcc ggttcaagca
    31381 tttttttcta attatcccga aatagatgtt tttattgaaa atgcttacaa tttggttaag
    31441 ccggctgtag aggtgtatgt aagtcgggga tttcagcacc tgatggtgag ttttggttgt
    31501 accggcggga ggcatcgctc ggtttattgt gctgaaaagt tggctgaaaa gcttaaagga
    31561 gtagagggcg ttgatatttt tttaagacat ctggagcagt aaaaataaac aggatttaat
    31621 cagatcaaaa aaaaacaatg atactaagtt tctatttcat aaaattgtat tactgatttc
    31681 aacaatccat ttgcttttac cgtaaccgtt ggacttttgg ttttatggaa aataaatttt
    31741 aactctgagt atgcactcgt taaagcaagc tctaattttt gccgccggct tgggtaccag
    31801 gctgcgcccc ttaaccgacc ggattcctaa agctttagtg gagataaatg gcaagccgat
    31861 gcttgagcgt ctgattgaaa aattgatagc agcaggaatt caaaacattg tgataaatat
    31921 tcatcatttt tcgaaactca ttattgattt tgttgagaag cataatagct ttggggtcaa
    31981 tattcagttt tctgacgaag ccgaccaact gttggataca ggaggcggct taaaaaaagc
    32041 attttcattg ttcgatccta atttacctct gcttttgcat aatgttgatg ttgtttccaa
    32101 tgttgatctt aatcaattag ctgagtttca taaggaaagt gaatctattg caactctttt
    32161 tgtccaaaaa aggattagct cccgctattt gctttttgat gaaaacctaa aattatgcgg
    32221 ttggagaaat tataaaactg gtgagaagat tttggttttt aataatgatg ataaattgat
    32281 tgagatggct tttaatggaa ttcatattgt gaatcctcaa cttgttaagc taattacaga
    32341 agaggggaaa ttttccatta ttccggtcta tttgaggtta gctttgtcat ataaaatttt
    32401 cggttttcgg aatgacgatg cattttattt agatgtcggt aataatgaaa atcttgccaa
    32461 ggctatttct ttattaaaaa aatatgatgt ataaagccat tcctgttttt tttgaacaac
    32521 aaaatggaga gattggtttt cgtaagaaaa tctatttttg caaaataatc ttacttatta
    32581 ttacccatga atttaagttt atattttcct aaacttttga tcaaccttgc catcctgatc
    32641 ccaatgtgtc tgcctatatc cttggaggca caatggcatc aaatgtcgaa ggaggaatac
    32701 ataaaaaacc ataggaattt ggcaatcaaa gagatgtatg agaatggtat tccggcaagc
    32761 ataaagcttg cacaggcaat cattgaatcg ggtaatggaa atagcaggct tgcgcgagag
    32821 gcaaacaacc attttggagt caaatgtcat ggctggaacg gcgatataat aaacatagac
    32881 gacgatgttt tacaagagtg cttcaggaaa tacagtgatg ttgagcagtc gtatagagat
    32941 cactctctgt ttttgatttc aagacctcgt tatagtcaac tctttgagtt ggaaactact
    33001 gattacgcgg gatgggctaa tggattgaaa caagccggtt atgctacaaa tcctcgttat
    33061 gccgaaatac tgataaaaac tattgaagat tttgaactct atcagtacga tgtttctaaa
    33121 agtgaaatat tggcatctgg aaagctaact gaagagcctt tagaccctga aaatataaaa
    33181 gataaaacaa cctcccgacc gttggcaagt aacaggactt tattagtaaa caatgggcgc
    33241 aagtatatct ttgcccgtaa gggtgatagt ttcgcttcac ttgctcagga gtttaatata
    33301 tacagctttc agatttggag gtacaacaac ttggataaac gcgacaagct gaaagaggga
    33361 gacatggttt atcttgaaaa gaaaagaaat aaagccgcct cgccttatca tattgttgcg
    33421 agaggagaaa caatgcgtgc tatctctcaa cattatggta ttaaacttaa aaaactttat
    33481 tggtacaatc gcatgaacca tggtgatgaa cctcatgtag gtcaggcgct atggatgcaa
    33541 cagcgtaaac caattgaata acctaatctt gcctggtaaa ttaatggttt tttttaagtt
    33601 tttcattttt atacttatgt aaataatgaa tcaagaaatt tatttttacc tttgcgccca
    33661 attttttttt gaaaaggatt aagtttttat gattaatata acctttcccg atgatagtaa
    33721 aaagcaatat gataaaggca taacaggcct tgatattgct aaaagtataa gtgaggggct
    33781 ggccaggaat gtgcttgctg ctgaggtaaa tggagaggtt tgggatgcaa caaggccaat
    33841 aaatggcgat tcatccgtaa ggcttctgac gtgggatgac gaagagggta aagctactat
    33901 gtggcactca tcggcacacc ttatggccgg agctatcgaa actcttttcc ccggcgccaa
    33961 gtttggaata ggccctgata tcgaagcagg attctattat gatgtagata ccggcgatcg
    34021 gcagatcact gacgcagacc tgattttgat agaaaataaa atgaaagaga tggtcaaaga
    34081 gaagcattcc ttcacaaggc gtcaggtttc gaaaaatgaa gcaatagatt attttactaa
    34141 aaagggcgat gaatataaac tcgaacttat cagtgagttg gaggatgggg atattacctt
    34201 ttatgaatcc gggaattttg ttgatctgtg tcgggggcct catttgccta ataccggcta
    34261 tataaaagca ataaagttgc taagcattgc cggcgcctac tggcggggag atgaaaaacg
    34321 taaacagcta acacgtgttt acggtatcac tttcccgaaa gcaaaagagc ttgaagatta
    34381 tttggtgatg cttgaggagg ctaagaagcg tgatcatcgg aaattaggaa aagaattgga
    34441 gatttttacc ttctcccaaa aagtaggatt gggattaccg ctttggctac caaaaggagc
    34501 tgagcttcgt gaacgccttg aacagttttt gaaaaaaaca caaaaagagg caggttattt
    34561 accggtaatc acacctcaca taggtaatgt cgagctatat aagacctcag gtcattttgc
    34621 aaaatatgga actgagtcat ttcagcctat ctctacacct ataccgggtg agcagttttt
    34681 ccttaaacca atgaattgcc cgcatcattg cgaaatatat aaatcgaaac ctcattccta
    34741 taaagacctt cccttacgct atgccgagtt tggaacggtt tatcgttacg agcagagtgg
    34801 ggagttacat ggattgacaa gagtacgtgg gtttactcag gatgatgccc atattttctg
    34861 tacacccgat caggtgaaat cggaattttt cggggtgatg gatatcgtga tgaagatatt
    34921 taaagctttg gattttaaag atttcattac acaggtttcg ttgcgcgatc caaataataa
    34981 ggagaaatac atcggtaccg atgagaactg ggaaaaagct gaaaatgcca tattggaagt
    35041 tgcgcaggag tcgggattag aaacggtggt agcatacggc gaagcagcgt tttacgggcc
    35101 taagatggat ttcatggttc gtgatgcatt gggaagaaaa tggcagttgg gaaccataca
    35161 ggttgattat aaccttcccg atcgctttga attagaatat acaggaagtg ataacgaaaa
    35221 acatcgcccg gtaatgatac acagagcgcc gtttggttcg atggaacggt ttgtggcagt
    35281 tttgatcgaa cattgtgcag ggaaattccc actttggctt actcctgttc agtctattat
    35341 tttaccaata agcgaaaaat atcaggagta tgctgaaaag gttttatttt tgctgaccgg
    35401 tttaaatatt cgggctgaga ttgataaccg caacgaaaag actggaaaga aaatacgtga
    35461 cgctgaaatg aagagaattc cgtttatgct tatcgttggc gagaaggagg aggccgataa
    35521 tactatttcg gtcaggaagc aaggcgaagg cgatctggga acatttacca tcgagtcgtt
    35581 ttccgattat attgaaaatg aaattaagaa gttaacagac aaataagatc tatttattaa
    35641 ctaaaatata ggaggataag ttatagtaac gcctttatca agcaaaaccc caaaacggtt
    35701 tgttccgcga aaacaggaaa atccacatag gatcaatgaa gagattaaag gacctgttgt
    35761 tcgattagtc ggggataata ttgagcagag cataatatca gtaaaagagg ccattgaaat
    35821 tgcaaaacaa catgaacttg atctggttga aatttcaccg gcagcaaacc caccggtttg
    35881 taaattgatg gattacaaaa aattccttta cgaccagaag aaaaagcaga aagagataaa
    35941 agcaaaagcg gccaaagtgg tggtaaaaga gataaggctt gggccaaata ctgatgatca
    36001 cgattttaac tttaagctta atcatgcgaa aaagtttctg gaggatggag caaaagtaaa
    36061 agtagaagtt tttttcaggg gaagaacaat tgtttataaa gacaaaggag agctaatgct
    36121 gctcaagttt gcacaggagc ttgaggatta cggtaaagtg gaaaagcttc cattgctcga
    36181 aaaaaagcgg atgataatga ttatcgctcc aaagaagaat tagaataaga gatgtactaa
    36241 ccattttaat atttttgaat aatgccaaag atgaagacaa aagccagcgc taaaaaacgt
    36301 tttacgttta caggtactgg taagatcaaa agaagacatg cttataaaag tcacattctg
    36361 actaaaaagt cgaaaaagcg taagagaaac cttggctatt ttgccatagt agataacgct
    36421 gatgcgaaaa atgttaaagc aatgcttgta aaataattaa tcaattgtcg aacttgcgtt
    36481 cagcggatta agaccccgaa gaagcggggc gctgacgtaa aaaattaaaa gtaatatgcc
    36541 aagatcagta aattcagttg catcgcatgc aagaaaaaag aaaattttag cgcgtaccaa
    36601 aggacgatat ttagcgcaaa aaaatgtctg gactgttgca aaaggctctt acgaaaaggg
    36661 cttgctttat gcttatcgcg acagacgcac aaagaagaga aacttcagag ctctatggat
    36721 acagcgtatc aatgctggag taagaccgta cggactctct tattctgcat ttattgggaa
    36781 acttaataag tccgagataa aacttaatcg taaaacattg gccgacttgg ccatgaatca
    36841 tccggaagcc tttaaggctg tagtggatac agttaaagga taacaaaata aagcaggttc
    36901 gcctgctttt tttattaata gcatttcctt ctgcttgatg aatatcaaag ccattttaac
    36961 ataaaatgtc ttaactttgc tggaagtctt ttttaatcct ctctcttaat gacaaaaatg
    37021 ctctcctata ttcgtggtgc aacgtttata atcgtactct ctttatgttt tgttactaaa
    37081 ttgggcaaag cacaggagag cgctattgtg gccggtaggg ttattgctca gacaacaaaa
    37141 actccaattg cttttgctga tgtctggtta aagccgggca gactaaatac gataactaat
    37201 agcgctgggg agtttgaaat tcggggagta aaatcgggaa attatgagct atgggtgaag
    37261 tctgtcggtt ttgagagctt tagccaaaaa gttgaaataa tagagaattt gcctttttac
    37321 ttcagtattg agcttaaaaa tgatgtgagg tttattgaag gagttgaaat taccggtcaa
    37381 cttacccgtc agggatcata tttgagagag gtagtatcgg caaaggagat tatgcgtgtg
    37441 ccgacgcgtg atgtgggtga atacttgagg gagacgccaa acatatccgg tattagaaaa
    37501 ggcgctacaa atatagaccc cgttttgaga ggaatgaaag cgggacaatt gaacgttcag
    37561 ctcgatttcg gtcaaaaaat tgaaggcggc tgtccgaatc gcatggatcc ggcatcatca
    37621 catgttgacc ctaatgatct tgcttccgtc gaaatattaa aaggaccata cgccttgcgg
    37681 tatgggccta tctttggggg cattatcaac cttgtgtcga aaaaacctat acctaatgag
    37741 ggattcaaaa ttgggataaa agcatctaaa gggtgggaaa ccaattggaa cggtaatgaa
    37801 gaacacctgt cggtttatgg tggaaatgaa aaaatttatt tcctcatggg aggcaataac
    37861 agatattatg gaaattataa agctggtaat ggtgaggagg ttaaatcttc atttaaaaag
    37921 tataattatg ttgctaaggt aggcgtaacg cctcataaac atcataactt cctgctctct
    37981 tatgataact cacacggtgt tgacattcgt tttccggctt tgccaatgga cgaacgtaaa
    38041 gatgacacta aacttgcctc tttaaagtat cactatttca atccgtcaaa aagtcttcaa
    38101 tctgttgatt taatggttta ccgatcggat gtgttgcatg aaatggataa caaatggcga
    38161 cctatttccg atactgtggt cggagtttca aaggttaatg cgcttaatca tggcgcaagg
    38221 attgactttg gtgtgataaa aaatgataat acgctacatt ttgggttgga ttatgaaaac
    38281 atatttaaag atggaaatcg aaccaaaaac atgatacttc agcctaattt accggttaaa
    38341 gttgagtcgc tctggaaaaa cgccagaata gagaatttag gagttttttc tgaatatcgg
    38401 aggaggcaat ctgacaatat gcaatggatt attgccggta ggttagattt taacagcggt
    38461 atctccgatc cgcttgccct gatggacatg aaagggaatg cgatttactt taatgataca
    38521 gtcagttctg gttttttgaa ctttagcttt tcaacgggtc ttagttatat aatagataac
    38581 catcttacga ttgatttttc cgtaggccgt ggaacacgca gtcccgatat gcttgaacga
    38641 ttcattattc tgttacctgt agggtacgat aattacgatt atcttggaaa tccgcaatta
    38701 aaacctgaga gcaatcatca ggccgacctt acttttaggg tctctgccgg cgaatttgga
    38761 gaaatgacgg ctaacggttt cttctcatac attaccgatt atatcagtgc gatgcggata
    38821 ccgccatcgg tagcgctacc ccaaactaat agtgtttatg gagttaagcg gtttacaaat
    38881 ataaattacg cgctgatgta tggttttgag tttcagtggg aaacaccatc tactaataat
    38941 tggaaatcaa accttgcagt ggcttatact accggaataa accctgaggc aatacatttt
    39001 attatggaga atggagaggt tacaggaagt gaaaaagtaa taaacgatcc attgcctgaa
    39061 ttgcctccgt ttgaagggaa ttggaaattg ggatacaacc tttttaacag acgtttcata
    39121 ccggaactga atattaggat ggttgccggt caacatcgga tttcaaaggc ttacgatgaa
    39181 gaaacaaccc caggatttgt gatattcggg attaatatgt tttatcgtta caatcgacag
    39241 atatctatct cggcaggggt aaaaaatcta tttgatacct tttattacga gcatctgaat
    39301 aggagaatga tcggtacctc tgcttcgatg tatgaaccgg gacgaattgc ttatttcaaa
    39361 attatgatag acttataata tgatgaaaag gattttttgg ctctttcttt tattagtgct
    39421 tgttttacag gcttgcgaaa tgcgttcgaa aaagtcggtg aaatatgttg ccacagatgc
    39481 tatttcggga tatgacataa gttatcgcaa tgagagtggc aatctggtaa aaaaaagagt
    39541 tgaagtaaac agcgcccttg aaagatggga atattcattt attgctgaac aaggagatat
    39601 agtgtatgtt tcaggaaatt ataaagatgt tgcttcggcc ttgaaaatca tgatattgat
    39661 agatggtaaa atttttaaac aagctgccac acggcacgac acagtgaggt atgttacagt
    39721 ttcaggagtg ataccttatt agtattctta accgcaacgc tttgttgtaa gtcttataaa
    39781 caatagtttt agataacgaa agttttaaat tgataagaga tcattttttg ttaatgctat
    39841 gcgacaatac atttatctgc tgatcgccct attgcttgta tcgtgcaacg gatcaaaaga
    39901 tgaccccgaa ttggatatgg gtcatatcac atttcatttt actcattacg ctgatgatga
    39961 tcttctgata tttgatgaat tgatttatac caatgccgct ggtaatcaat atatggttaa
    40021 tgagattcag tattttattt ccgacgtaaa attacatcgc cgcgatggta cttatcagct
    40081 aatcgaagag tggaaagata tccactatgt tgatactgat ttactcgaaa ctcaaagctg
    40141 gaaagtcttt gataagatta agccgggcgg ttatgataaa atctcttttg tttttggcat
    40201 atcagaaagt aaaaacaaaa gcctcatgtt tgtcaatcct ccggagcgag atatgttctg
    40261 gcctgaagtg cttggcggag gatatcacta catgaagcta aacggcaaat ggcttactcc
    40321 agagcagcaa gtttttcctt ttaattttca tcttggaatt gggcagattt atgccggcaa
    40381 cgttattcat gtagattcaa ttataggttt tgttcaaaat tactttgaag tacagcttgc
    40441 aaattcttca tttgaaattg gggcagggga cacccttcaa tttgaaatca ggatggatgt
    40501 tcagaattgg tttcaacacc ctcatattta tgatcataac caatggggtg gcgacattat
    40561 gcaaaaacag gaggcaatgc agataggttg tgaaaatggc aaagttgatg tcttttcaat
    40621 tttaaggaaa tagagagtaa gtgttggttt tggttttagc gccgataacg agtatcctat
    40681 ttactgaaaa atgctcacac cctacgtccg atcaattttt ctacagataa aaagataaaa
    40741 aaagccctta attaagttat ctcacgatta ttattttttg atttgagatt gaactctttg
    40801 tctctatcct taataaataa attccgggtt ttaattgatc aacaggtaag ctgatgtttg
    40861 aattttcagt gtttacaatt tgcaattcag tttttcccgt caggtttgtt aatgaaataa
    40921 gggcatttgt gtcggcagga tcgagaaacc taatgttaag catatcgctt gccggaatcg
    40981 ggtaaacaag taattgtgta ttgagtatgt ggttggatat tcctgttgaa tagtgcatca
    41041 gtgggaaaat ggcatgatca atattgatat tccagctaaa ctgctgattt gagtacggat
    41101 accagttgcc attatcatgt tgctcccatc cattgccatc tataacgtct ccatctaaat
    41161 tggtgtaaag tgcaagagtg tcgccataat ttttgggcag ctctactcca agaaaaaatg
    41221 gttggttcat ttccggagga tcgttaaaaa atagcagagt ggaccagcca aatcctacct
    41281 cttgtactat atgtgataag aaaagggtgt tctcggcaag tgtttttccg ggtgatcccc
    41341 cttcgccatt acaatcccat gctttgaaag taatctgttt atcgctggcc atcgagcgtt
    41401 tagctactgc aaaatcgaat attccaccaa taagtttgat ttctgactca gaattatttt
    41461 ggaaataact tgctttggct aagtcgccat acgagttgtt gcccgacacg taaccacttc
    41521 cattttggct gataacataa agagtatagt cgccggcaag aggataataa agagtgtcgc
    41581 aggagcttcc agaaactacg acaatgtaat tgttccgggt gagggatgaa gattctgatc
    41641 ctttggtgat actaagtttt actgtgtaat ttccaggttc ctgataaaca tgaagcgggt
    41701 tttttacggt gtcagaattc ccatcgccaa aattccagtt ccagccggtt ggttgcccct
    41761 ccgaggcatc cttgaattgt actgtcattg gcgctgaacc aagacgatca agggttataa
    41821 aattagctgt cagattttta gaatcctcgc ttttattact tgctacagcg acctgtttat
    41881 gatgttttgt caaaaggtca gcattgcgtt gactaaaaga gactaaggag ctaagaagca
    41941 gtaaaattaa aatagtgtgt tggattatca atttcataat gcaatttttt attgcaaagg
    42001 tatgctaatt tattaatgtt gtgataaaat atttattctc agcacttgaa taactaccta
    42061 aacccttatg gtatttacac tttgttagcg tttcgttttc catttttctt gatgtttttc
    42121 tttcattttc gcttgcttgc attaatgtgc cgctgtgtaa agtccttatt gtttgttgtt
    42181 ttttgcactg atacatccga atgctttatt gccggtaaag gtctcacgga gctttgcacg
    42241 gagttttcaa tcgaaagaag tagcattttg aagagaacta acccgctata gttttttttg
    42301 ccacaaagat tataagcctt actgtagtaa ctattaataa ctgtttttac cgatttccaa
    42361 ttgattataa aattcaaact atttatcgtg caaaggtctc ggtctttata tccacatcgg
    42421 ctgggcagta gctcgctgca aagtggtggt ggagtaaaca ggaaaattca tggtgtgtat
    42481 tcagtaagtg cggatttgtt tgttgaaaaa ccgtatcaaa aaaaaatcga atgctattag
    42541 cgcaacaaaa cacccatgcc aactaattta aaaatgctct ttagcagtgc attttaattt
    42601 tatgatttag cccgtagggt tattaacaag aaattttgtt gattactttt ccgaattggt
    42661 taagaaatat tggcatgctt atactatttt tgcctgataa tgatttatta gattgatgtg
    42721 taagattttc atatttagaa tttcgatttt ttttgccata gtattcgttt cacaaggttt
    42781 atttgcgcaa gaatatcaaa aggttgaggc tgaaagcggc gaggggattt atagtttgct
    42841 aaggaggttc aatctcgacc ctaaggatta ttataatacc tttttggaga tcaacaaaaa
    42901 ccggcttaat cctgatttgc aacttttaaa agggaagact tactttcttc ctattgttgt
    42961 tgatactacc gcgttggaaa ctgaaaggca aattggtgtg tttccaattt ttgggaagaa
    43021 gtacgagaag gttgagttca aggatgacaa attgcagggc gccgtgtttt atattatctc
    43081 aggccacggc ggccccgatc ctggagctat gggtaaaaag aatggccaca ccttatgcga
    43141 agatgaatat gcttacgatg tctgtttgcg cctggcgcgg aatctgatcg aacatagcgc
    43201 cactgtttat attattactc aagaccctga tgatggtatt agagatgaag agtatctgaa
    43261 atgcgataaa gatgaaatgt gccttggcaa taaaaaaatc cctttgaatg ctgttgaaag
    43321 acttaaacaa aaagtggaaa cagtaaatca gctttttgaa aagcatcttg gccagtatca
    43381 acgtaaactt gagatacatg tagattcgcg ctattccaat cagaaggttg atatctattt
    43441 ttattaccac cccggtagca acaagggaaa acagttgggc gaaacattgt taaaaaccat
    43501 tcatgataaa tacaagcagc atcaaccaaa ccgtggatat aaaggaactg taactgaacg
    43561 aaaattatac actctaaaaa atacaaaacc ggtatcaacc tacattgaac tggggaatat
    43621 caatcaccca agagacttgg agcgcttaat aattgtggat aaccgtcagg ctattgcaaa
    43681 ctggctcacc ctggggctga tcaaggattt tgagaattca aagagaagat aggcgagtaa
    43741 gttatccgat agcgctatcc gttcagttaa gaggtttttt tataaaaggc aaaatagcac
    43801 gctgatatac tccctgcatg taggcaattg ccattccgta gtttgttaca ggtatgccgg
    43861 catcaatagc tggttttaag cgatttacca tctgttttcg agttatcata cagcctccac
    43921 attgaaccac caacgcataa tcggttattg ggcgtggcag ctgatttaaa cctgaaacca
    43981 cttcatattt cagcttttta cctgtaaatt tatccatcca gtttggcatt tttaccctcc
    44041 cgatatcttc gcaagaaaca tgatgcgtac acgattcgag tatgaggact atgtcgcctt
    44101 cttttaacga tgagattttt ggagttccat taagataggc gtcaaaaggc cctttgtatc
    44161 tggcgagtaa aatgctaaaa ccagtgagag gtatcgtttc aggtattatt ttgctggctt
    44221 ctaaaaatat agagctgtcg gtgataacaa gagccggctc aaccgctgtt ttctttaaaa
    44281 aagattctgc ttcatgttcc tttacaacaa ctgcaacgga gtcgtggtca agtatatcgc
    44341 ggatagtttg cacctgagga aggattagcc ttccctccgg cgctgctata tcaatagggg
    44401 taattaataa tacgacatcg cctttattta ttaactcgcc caatagggtg gggcggctcc
    44461 aaacggtttc gggcattata gaccgaatct tttgcaaaag atactctaag ttgtttggtt
    44521 gtaatgcgtt gaactctatt attggctgtt tatactctga ttcaagatgt tttgcaaaat
    44581 ctttgtcgag cggcgtgata tcacttttgt tgtgtatcca taaaaagggt acgccataat
    44641 cgtaaaaata gccgatgatt tttttctccg acataccgat agtgttatcc gaaatcaaga
    44701 ggagagcaat atctatttgt ttgacgattt caattgacct ttctatcctt ttggcgccca
    44761 attcgccaat atcatctatt ccggcagtat caaccaatat tactggccca atacccggta
    44821 tttccattct tttttttaca ggatcagttg ttgtacccgg tgtttcggat acgatagagg
    44881 tttgctgtcc ggtaagtata ttgatgattg agctttttcc gagatttgtt tttccgaaaa
    44941 ttcctatatg tggcgtgctt tcgcgtggtt ttatcacggg gctgtttaag ttcataacag
    45001 gttaattata gtaagttttt atcggttaaa ggtaagtcgt tggccacggg cagacaaatc
    45061 gaatttgccc atttcataaa ctaaattacc atttacaaaa gtatgtgtta ctgccgattg
    45121 gaatagcgtt ccttcaaatg gcgaccaacc gcatttgtac agcaggtttg atttttcgac
    45181 gatgcctggc tgattaggat taacaattac gagatctgcc ttgtatcccg atcggataaa
    45241 tcctctcttg ttcactttaa aacaaattgc cggtgcatgg cacatcttct caacaatttt
    45301 ttccatagat atcttacctt gatgaaaaaa ttccattaat gcaattaatg agtgttgtat
    45361 tagtggtccg cccgaaggag ttttgaaata actgtttctt ttttctacta atgtgtgtgg
    45421 tgcatggtcg gtagcaatta catcgagccg gttgtcaagt agagccttcc atagagcccg
    45481 acgatcggtt tcggttttta ttgcaggatt ccattttatt aaatttcctt ttgtgtggta
    45541 agcctcatca ttaaaccaaa gatgatgaat gcagacttcg gctgtgatac tcttttccgg
    45601 caaaggaata tcgtttctga aaagcgtggt ttccaatgct gttgagagat gcaaaatgtg
    45661 aaggcgggta ttatattttt cggctaactc tattgctaaa gaggtggata tgtaacatgc
    45721 ttcatcacta cgaatttgag gatggtaatg aataggaatg tcgtctctat aaatgttacg
    45781 caatctgacg atgttatttt ggatggtagc ttcgtcttcg caatgtgtgg ctactaaaca
    45841 aggagcctcc ttaaaaatag cttgtaatgt tgatttatta tccactaaca tatttcctgt
    45901 ggaggcgccc atgaacactt ttatgccaca tacattcaca ggatcggttt ttactatctc
    45961 ttttacgtta tcgttggagg cgcccatata aaatgagtaa ttagccagcg atttttctga
    46021 tgccagctta tatttttctt caagcaaatc ttgtgtcagg gtgttgggaa gcgtgtttgg
    46081 catatccata aatgatgtta ccccaccggc tattgcagca cggctttcac tgccgatatc
    46141 ggctttatga gttaaaccag gttccctgaa atgaacctga tcatcaatta cacccggaag
    46201 aagaaacata ttggaggcat caataaattc gtacccctta aaactattga cttcaatatc
    46261 gtcagtatgt tctaatattt tttcaatctc ttcaccctta gtaaataccg aacctttgaa
    46321 aatacgacct tcgttaacga tatttgtatt tgcaatcagg taattcattt tctattggtg
    46381 tctggtaatt aaactttgta aaggtttagc tttttctatg ttataaattt attggtgaga
    46441 tgaaaaattg ttttatctaa ttttttttgt tccggtaata gttttttaaa aagctagtaa
    46501 tattgttttc aacccttttc aaaatgtcgg atgcgtttgc tttttgaaaa ctttcaccgg
    46561 ttattctttc aaatagttcg atgtaacgtt ctgatattga tgaaactatt tggtcggtca
    46621 tctttggtat tttttcaccc ctatcgcctt taaagttatt agcgagaagc cattcccgta
    46681 caaactcttt ggagagttgt ttttgtggtt cacctttctt ctgacgttcg ttataccctt
    46741 ctaaataaaa atagcgcgat gaatcggggg tgtgtatttc gtcaatgagg tagattttac
    46801 catctctctt cccaaactca tattttgtat caactaaaat gagtcctttt ttcttagcta
    46861 tttcggttcc tcgtttaaat agttctcggg tatatttttc aactaaggca taatcctctt
    46921 cagagactaa tctttggtta attatgtctt ctttggaaat atcttcatca tggccggatg
    46981 tagcttttgt tgtgggtgtg attattggtt ctgcaaattg ctgatgttct ttcatgccat
    47041 cggggatcgg cactccacat atttgtcgta ttcccttttg gtaggttcgc catgcgctgc
    47101 cggtaagata acctcttatg atcatctcta cgggaaaggg gtcgcaatgc aaaccgatgg
    47161 ttacaatggg atcgggaacc gatattttcc aattgggaac aatatctttg gtagcatcca
    47221 gaaacaaagc agctatctga tttagtacct gacctttata aggtattcct tttggcaaaa
    47281 cgacgtcaaa ggccgaaata cggtcggtag ttatcatcac aagtaattcg tcattgatgt
    47341 tgtaaacatc tcggacttta cctgtgtaaa ggcttttttg gccatcgaag ttgaaattag
    47401 ttcttgttat ggttcccatt attttgcttg ttgttaccgg tttttggttg gttgctgtat
    47461 gcatttatta tctgtaccac taacttatga cgtataatgt ctttttcatt taggtaaata
    47521 aaatctattc cgggtatgtt gttcaggatt cgcgttacct gaatcagccc tgaggattga
    47581 tttcgcggca ggtcaatttg tgttatgtcg cctgttacga taaattttgc ggaggctccc
    47641 attcttgtca ggaacatttt gagttggttc tccgtagcgt tttgcgcttc gtcgaggata
    47701 gcgaatacgt tgtcgagcgt gcgccccctc ataaatgcca gcggtgcgat ttcgatggtg
    47761 ccctcttcca ggtagcctaa taatttttga gaggggagca tatcgcgaag tgcgtcataa
    47821 agcggttgta agtagggatc taatttatcc ttaaggtctc cgggcaggaa gccaaggttt
    47881 tcgccggctt caacagcagg ccgggtgagg attattcgtc tgatctcttt gttgcgaagc
    47941 gcctgaactg ccagagcgac agcagtgtat gtcttgcctg ttccggcagg cccaatggcg
    48001 aaaatcaaat cgttgcgacg gcaactgtca accatccgct gttgattggg tgtgcgtgct
    48061 ttgataagtt tgccgttacg gccatgtaca agcacatcgc tgccaaactg gaggtcttgt
    48121 ggcgtataat cggattcgga cgaaagaagg tcgtttacat catattcgct tagcttgccg
    48181 aaacgctcaa gatgaaggat tagcaaagcg aatttttttt cgaatgaaat aatttcagct
    48241 tcatcgccaa tgacctttat ctcggtgcca cgagctatta atttcagttt gggaaagtgt
    48301 tttttgagta tcttaaaatt ggaatcatta actccaaaaa catcaagcgg attaacggat
    48361 tcaattagga tatttttttc ggtcattaaa aaatacgata gataataata tttttttgtt
    48421 caaaatttga aagttcaaaa ttaccaaaaa aatgttcatg gcatgtcagg gaaatcttat
    48481 ttttgcccgt gatttaaaaa tcagacattt aggtttcatg gctctcattg cattgctctc
    48541 cgactggggt acgaaagatc actatgtagg tatggtgaaa gggaaaatac tgactaaaat
    48601 acctgaatcc gacatcatag atatctccca cgaaatacct ccgttcaatc ttaataatgc
    48661 agcttttgta ttgagacatt gttaccgtca gttccctgaa aaaaccatat ttattgttga
    48721 tattacatcg gaagcgacaa tcgaaatgcc acatgttgtg gtggaatatg caggcagcta
    48781 ttttatcggc gccgacaatg ggattttttc acttgcattt gatgaagatc ctgattctat
    48841 caccgaaatt gacatttatc aggatacgca tacattcact tttgccgctt ttgacttatt
    48901 tgtaaaggta gcacagcata ttgtagaagg aaaagagatt agcctcttag ggccgaaaag
    48961 agataggctg gccgaaagga tccagctaaa gcctgtggca gaacctgatc tgataaaagg
    49021 tcatgttacc tatgttgaca attacgaaaa tgtgtttacc aatattgatc aggagctttt
    49081 tttacgtata gggcagaagc gatcgtttag gatactcttt ggcgcatcac gctatagcgt
    49141 taggcagata agccagtcat acaaagatgt ttctgaaggc gagatagtag ctctcttcag
    49201 ctcttccggc tttttgcaga tagctgtgtc aatgggcaat gcttctggct tgttagggtt
    49261 gaaagtgaat gatatgatac gtgtagagtt tggataaggc ggatcaacat cttaaaatca
    49321 ggtagtacct cataaactta cacagtttat gagatactac cgtatttttc tcatatggct
    49381 gttgtttaaa atgttttcta ccgcttgtta taggctggtg ttcattattt tatgtcattt
    49441 tatctttcaa tttgctactt cgtttctgtt tgtcgtgcgt tggttcatgc agtctgcaaa
    49501 tgtgttgttt tttgcatagg ctattcatta tatggttttt ctttttctgt ctttaagtgg
    49561 caaattgtct gttatgtctg gttttttaca cttgcccttt aacatcggct ttattccttt
    49621 tctttaatca agtaaatata aacagggaag tggtcgctat aaccgccaag atagactcca
    49681 ccggcaaaag ttcgccaggg ataccccttg aaagcgccct ctttttgtat taaccagggt
    49741 ttattaaaca ctccggcacg gtaaaaagta taagttgatt tgtcgtctcc aatcaaagcg
    49801 ggagtaagga taatctggtc gaaaagattc catgaatcgc gataggcaag gcttccaatc
    49861 ccttgacggt aaaggctttc catggggttg aacagtgtgc ctggttttat ctttttaacc
    49921 acaccaaccg tgttaagtcc ttttcttatg gctttatctt taggatcatc gttcatgtcg
    49981 cccattacaa ttactttcgc attggggtca agcgctaaca gtgaatctac tatcgaacgc
    50041 gtgagttgag ctgccgccat ccgtaacggc tcactcttct tttgaccact tcggcgcgat
    50101 ggccagtgat taactataaa gtgcatcatt tcgccatcga acatgccgga aactacaagt
    50161 tgatcgcggg taaaaaacat ctccatacct ggaatgatca gccgttttga ttcagagttc
    50221 gttaccgtga agtatttagg attgtaaagc aaggcacaat caacacctct taggtcgggc
    50281 ccgtcgtaat gcacaatctg ataattttgc ctgcgaagat gttcttgagc tacaaggtcg
    50341 tctaatacta ttctgttctc aatttctgac aggccgatta ttgcagggcc gtcaggagtc
    50401 agatcggttc ctatttttga gataacctct gaaatccggt caagtttttc aaaatatttc
    50461 tttgtattcc attgaattgt gctctccggt aaaaattctt catcaataac tcccggttga
    50521 tttatggtat cgaaaaggtt ttcgatgtta tagaaagcaa tgcaacctac tttatattgt
    50581 ttgtcgtttt gcgcatgtgt tggtagcgta agcagcgccg ttattataaa gatataagtg
    50641 gtgagtttca ttaaacctaa atttaaactt gagtaacaaa ggtgaaaaat ccagacgaaa
    50701 ataagtaaaa ttgaaaaatt agatacattt gcgcaattaa tagcgattta attaatcaac
    50761 caccaaccta ttattcaaca tttgctgatg aaaaattttt tactgctttc agctctttct
    50821 tttctgacat tcaatctatt ttcacaagta gatacaacgg taataaagtc ggaagaggtt
    50881 aatcttaatc aggtttatat gatcacttcg gcggatcttg aatctgacga acaacctcag
    50941 gatatttcag ggttgttgca atcttcaagg gatattttca tttcgactgc cggattcact
    51001 tttggtcaga cccgttttag gatcagaggt tatgattcag agaatactac tgttttgttg
    51061 ggcggagttc ctttgaatga catggagacc ggccgcgcat attggtcggc ctggggaggg
    51121 cttaacgatg ttacccggtc gcaggaggtt aatttcggga tatctgcatc accctattcc
    51181 ttcggaggta ttagcggttc tgtaaatatt gagtctcgtg catcaaaaca aagacccgga
    51241 acacggctta cctactcttc gacaaaccga gcctatcgca accggataat ggctacttac
    51301 aacagcggta tgatggataa tggttgggca atctccgttt caggatcaag acgttgggca
    51361 aatgagggtt atgtagaggg cactttctac gatgcgtgga gttacttttt ggctttggag
    51421 aaacgaataa acaataaaca tagtattgga tttgtaggct tagcagcacc ctccaaggcc
    51481 ggacggtcgg ggcctgttac tcaggaggca aacgacttgg caggaaccaa ttattataac
    51541 ccatattggg gatatcagaa tggcgaaaag cgtaatgcaa gagtcggaaa ttacaatcaa
    51601 cctaagttaa tacttaccga ttattgggaa ttagataaat ctacaaaaat taccacgtcg
    51661 gcagcctatt tttttggaag atatagcacg acagctctcg aatggtttga tgcacccgat
    51721 ccgcgccctg attattatag aaagcttccc agctactgga aggataatcc acaaaagttt
    51781 aactatttaa ccgaacaatg gaaaaataat gagcagttta ggcaaataaa ttgggatttt
    51841 ttctatttcg ccaactctaa aaacctatat acaattgagg atgcctatgg aattgctggg
    51901 aacaatatta caggaaactt ttctaaatat gtagtggaag accgaagaaa cgatcataat
    51961 cattttatga tcaacagtaa tctttggaag gaaataaacc ataacattat cctcaatgca
    52021 ggaataaatc tttcatttta caaacagagg tcgtacaagg tacttgacga cctgcttggc
    52081 ggcgattttt atattgatct tgataaattt gccgaacagg attactttga tccatatcag
    52141 ggacaaaacg atttgcgaac acctaataga cctgtttatg agggcgatag cttcggatat
    52201 gattatgatg ccaatatcaa taattataac ggttttgtac aggctgattt taaatatgcc
    52261 aaaatggatt atttcattgc cgccactatc tcgcatacct ctttttggcg taccgggcat
    52321 atgcaaaatg gaaagttccc aacggaatca ttaggggagg cagaaaaaca aaactttttc
    52381 aattttggtg ttaaaggagg attgacctac aaaatcaccg gtcggcattt cattgttggt
    52441 aatgcaatgt atcttacacg agcgccctat ttcagagatt cctatatttc gccacgtaca
    52501 agaaaccatg ttttgagtgg tttgatcaat gagaaaattg ttgggggcga cattggatat
    52561 gtgttaagag caccaaaaat acaagcgcgg gcaacattat attatactga gtttaaagat
    52621 caggtctatt caagaagttt ttatcacgaa accctgcgaa gttttgtcaa ttatcaaatg
    52681 accggcgtgg atactaaaag tatgggcgcc gaaattggag ttgattataa attgacatca
    52741 acaataaata ttactggcgt tgcagggtta ggtagattaa cttataattc tcgtccgctg
    52801 gttacaatct ctcaggataa taattcagaa atcctggcag aaaaccgaat ggtctatttg
    52861 aaaaattatt atgttggcgg taatccgcaa acggcattat caggcggcat caaatacaat
    52921 gcacccaagt attggtggat cggagttgat tttaactatt ttgacgatat ttaccttgag
    52981 ccaaatcctg atcgtagatc agaggctgct gcaggaatat ataactccga agatcttcgt
    53041 ttcgaacaat tattggctca ggaaagacta tcctcagcct ataccattga cctgtttggc
    53101 ggaaagtcgt ggagagtaaa aaaatattac attgtattta atctttcgat caataacctg
    53161 cttaatgtaa cggattttgc tttcggtggg tttgagcaat tccgcttcga tccgaataat
    53221 attgacaaat ttcccccaaa atatttttat ttatatggta cacaatattt tgctaatttg
    53281 agtttacgtt tttaaaatca ataatttttg aaaaagctta attttattaa tatgataaag
    53341 agattaattt ttccagttct gatcattgca gtaattcttt tgttcggctt aacctcttgt
    53401 gtgaagcagg attttgataa gcctccttca aaagatatcc cggtagggaa ggtaattacc
    53461 ataaaggaat tgacttccat gtatcaaggg cagcccatag tttttgatac tgccatgtca
    53521 acttacggtg ttgttgttgg cgacgaatct tccggtaata tttacaaaaa ttcatacgtt
    53581 caggattcaa ccggcgctat taatttaaga ttaataaatc ccggtggttt atatgtaggc
    53641 gattcgataa ggatttactt gaaggggtta actcttagtg attatggcgg cgtcaggcag
    53701 cttgattcgg tagatgttga tttaaacatt gttaagctaa agacaaaaat aaatatagag
    53761 cctaaggtgg ttactattcc tgagcttgct ggtgggaaat acaattcaca gcttatcatg
    53821 ctcgagaatg tggagttttc ggctgctgat acggctaaaa cctggtccga cccacatggt
    53881 ttaacaactc agaaccgctc tttgatggat tgcgataaca atgttgtcgt tgttaggacc
    53941 agcggatacg caaattttgc tggcgttagc ctgcctaacg ggaatggcac ctttgtggct
    54001 atcgctagca tttttaataa tacaatacaa ctttatgtca gggattatgg cgaaattaac
    54061 cttgttggcg agcgttgcac cggcggtggt ggcggcggag gcggccaagg ttccggaact
    54121 cagtccgacc cgtggaatgt agcatacggc atagccaatc agaatgcaat gccatacgag
    54181 gtggggtggg ttaaaggata tatcgttgga tcagtaaaat cgggcgttac atctatcacc
    54241 tcaaatgatg atatattgta ttcaccgcca tttaacttgg caactaatgt acttattgca
    54301 gattcgcccg acgagaagaa ttatcagaat tgtgttatcg ttaatctgcc tgccggaaag
    54361 ccgctgcgct cggaggtgaa tcttgtagat caccctgata accttggtaa atggcttaac
    54421 gtatccggta cgcttcgcac ctattttgga gctgcaggat tacgagatag caatggcgaa
    54481 tcttctgact ttgaattgga aggcggcggc ggaggcggcg gtggtggaac cggttccggt
    54541 acacaatctg atccttataa tgtggaattc ggcattgcca atcaaaatgc gtcaccttac
    54601 atagtcggtt ggataaaggg ttatattgta ggagccgtca agtcgggggt tacatcaatt
    54661 tcatcaaatg atgatatcct ttatgatcct ccatttgatc tggcaactaa tgttttaatt
    54721 gccgattcga aagaagaacg caattaccag aattgtgtta ttgtcaatct gcctgccggc
    54781 aagcctttgc gttttgatgt aaaccttgtt gatcatcccg aaaaccttgg taaatggcta
    54841 aaggtaaaag gaacgctacg cacctatttc ggagctgcag gcttgcgcga cagcagcggc
    54901 gaggcctccg attttgagct tgaaggcgga ggcggtggtg gtggtggaac tgcctttttt
    54961 agcgaagatt ttaatactaa tctcggtgtt tttactgctt atagtgtaac aggtgaccag
    55021 gtgtggggat ggggaaactt cgacggcggt tgtgcagtaa tgtcgggata tgtgcagggt
    55081 acaaactatc ctaatgaaga ttggttggta tcgccggcaa tttcgcttgc aggcaaaaca
    55141 ggtgttaaaa tagagtttcg tgaagcaata aattacatca ctaacattag tgatatgaaa
    55201 gtgctgatct cgactgacta cagcgggagt ggcgatcctt ccatcaatgg tacatggact
    55261 gaattacaag gatttaaccg cgcgccggga aacaattgga attttgtaaa tagcggcgag
    55321 gtgagccttg catcatacga aaacaagact gtattcattg catttaaata tttaagttat
    55381 tcatccggct ccgcaacatg ggaagtgagc aaggttgagt taagagatta agagaacgcc
    55441 gaaaaagcta tatgagaact tgtttagcca tattgttttt tttcataacc atcattggtc
    55501 aaggacaacc ggtaggctac tacaacggta ctgaaggact acgcggtagc gctctcaaaa
    55561 ctaaattgca tgaaatcatt tgcgggcata acagtttatc ctattatttt tcaaaatatg
    55621 ttatctatta tgctgatgct gacccggaaa ttcccggaaa cgtaatttta atatataccg
    55681 gcaggtcgca ggatggtttt gattatggca ttggcggaaa tcagttgaac cgcgagcatg
    55741 tgtgggcgaa atcgcatggg cattttagcg ggatattacc aatggattcg gatgttcaca
    55801 atctcaaacc tgttgatgct tcagtgaact catcacgtag taatctggat tttgattata
    55861 gcctttaccc ccatcctgaa gcgacagaat gtaagtttac gccgggtgtt tcgtgggagc
    55921 caagagatgc agtgaagggc gatgtagcga ggactatatt ttatatggat gctcgttatg
    55981 aatggactaa tggtgaaatg aatctaactg ttgttgatca agtaaacact tatccgcagc
    56041 cggagcatgg caaactctca gcccttcttg agtggaatga gatggacctg cccgatcttt
    56101 ttgagtataa tcgtaataat gtagttcatc gttttcaaaa aaatcgaaat ccatttattg
    56161 ataatcccga ttttgttggc ctgatttggg gagataaatc gttgccctac ttttcgatag
    56221 gagatattgc gctttccgat gatcagccct acgaagatga gtccgttgtc ctttattgct
    56281 ccatttatcc ctctccggcc acagacaagg tgaaagtgat ggtcggaagt gatttcaatg
    56341 aattcgatta tgaaataatg atgacgttta ataacggatt atggcaagct gatcttttac
    56401 caaatgagga gggtgaagtt gtttattttg cagtaaaggc gggcgatggc gcaaatttat
    56461 caatatcgcc tacatattca tatcgggtgg cagcggcatg gggcgagcca ataacttcga
    56521 ttcaagaaat acaaggaact ggagatcaaa cgccttatca gaatatacaa gttactacaa
    56581 cgggagttgt aacctcattt cttcctacag gttattttat tcaggcaggt caggggccaa
    56641 ggtcaggatt gtttgtgtat gatccatcta gatatccctc tatcggcgat agcattgtgg
    56701 tatcaggtat tgctacagag tattatggcc ttacggagat tacaaatgtt tcgatgtata
    56761 aactgattaa aacggatcgt aaaatgcctg cccctgaggt tctcgattcg aaccagattg
    56821 gcgaggactg ggaagctgta ttaataagaa ttgagaatgc agagtgtacc tttacacaac
    56881 attggaacaa tagcggtatg tggagggtaa gcgatgacta tggtcaggtt aacgtgcaaa
    56941 ataatgacgt tttcagcatt gaccctgtcc tcaatgaaag atatacgata accggcccgc
    57001 tgaattatca aaattcaaac tggaaaattg agttaagata cctttatgat gtcgctgagc
    57061 ctgctagtgt aggagagaag actccaacag ttaagcttgc catctatcca aacccttcta
    57121 atgaaactgt tacaattgcc attcctccca atagaggcaa aaatccggta atcaggattt
    57181 tggatatttt gggtaatgag aaatggatta tacctgttga tccacacgat aatttattag
    57241 ttttgaactt gcttgagctg aatttaacaa aaggcgttta ttttgttatc tttgttgatg
    57301 accagattca gatttcagaa aagcttattt atttaccaaa ctaataaaaa gtaagcttgg
    57361 gttaaagtta ttttaaatat tgataatcaa cacttaaaat caaatgtcag aaaaattagt
    57421 tgttattcca acttataacg aaaaagaaaa tatcgaagct atccttactt atgtcctgac
    57481 catggagggt gattttcatg ttttggtggt tgaagataat tcgccggacg gtacagccga
    57541 tattgtaaag tcgttaatta aaaagtatcc ggacaggctt tttatcgaag agagaaaggg
    57601 taagttagga ttaggaactg cctatatcca tggttttaaa tgggcgttga accgtcagta
    57661 cgactatatt ttcgaaatgg atgctgattt ttcccacaac cctgatgatc tgcacaggct
    57721 attagatgca tgtgaatcgg gagctgatgt ggctatcgga tcaagatatg tcggtaatgt
    57781 gatcagggta gtaaattggc cgatggggcg ggtgatgatg tcatattatg cctctaaata
    57841 cgttcgtttc gtaacaggac ttaaaataac agactccact gcagggttta tttgttatcg
    57901 ccgtaaagtg ttggaaacaa tatggtttga tagaataaaa tttattggtt atgcttttca
    57961 aattgagatg aaactgacag cttcaaagct tggttttaac gtggttgaag tgccaatagt
    58021 ttttaccgac cggacagtag gaacctcaaa aatgactaaa gggattttta gagaagccgt
    58081 tttaggggta ataaaattga gatttaaaaa gatcttacct gttccttcta acaaaaatta
    58141 actggtaatc ctatcgagta ccaacccgat catctcatca attgttttat tataatctac
    58201 tgcaaaatca cctgtggcac gattggctat aacagcgcat attgttagtg cgtcatgtcc
    58261 gagtaatttc cctaatccat acaatgctga ggtctccatt tcgaaattca acaccctatg
    58321 tctttcaaag ctaaagtttt tgattttttc cgatagctcc acatgcctaa gcggaatcct
    58381 tattgtacgc ccttgcggcc cataaaagcc tggcgctgtt gccgtgatac cttgaaacag
    58441 atcatgaccg acttttttca ttaacttttc ggaacaagag acgatatatg gctttggtaa
    58501 attactgaag ctgccggttt gagctataaa ctctttcgtt agctcttttt caattacatg
    58561 atcgctatta taaaaatata aaagcccatc taaaccgact ccatgtgtgg aggcaacaat
    58621 agaatggatt gcaatatcgg gctgaatggc tccagaggtg ccaattcgta tcagatttag
    58681 cttggtgaac gattttttcg gctctcttgt tttaaaatca atgttagcaa gtgcgtcaag
    58741 ctcatttatg atgatgtcta tattgtcggt acccattccg gttgaaacaa ccgaaatagg
    58801 tatgccattg tatatccctg tgtttgttat tatttcacga ttttgacttt gatgttcaat
    58861 tttgctaaaa cgtcttgaaa tttttgaaac ccgggccgga tcacctacta aaattatatt
    58921 ctcagccaga atgcccggtt ttatgttaat atggtaaata ttaccatcac tatttatgat
    58981 taattcggaa tctttaattc ttttcatatc ccacaagcga ataattctaa ttttgcaata
    59041 aaaagattgc aaaagtaata ttaatccaga ctttaatatt agatggaagc agagataatt
    59101 acgattggcg atgagttgtt gataggccaa gtagttgaca ccaactcagc ctggttggca
    59161 gagcagttaa accttcttgg gataaaagta caccagatta caactatatc cgacagccac
    59221 gaagagataa taaaaaccct taccgaagtc tcccgacacg ctgatataat cttgttaacc
    59281 ggtggattgg ggccaacaaa agacgatgtt acaaaattgg cgctttgcga attttttgac
    59341 acctatcttg tgtttaatca ggatgcatac gaaaatgtgg aacgtctatt cagacaacga
    59401 ggctttttga tgaccgaaat taatcgccaa caagcatcta taccggcggc atgtactccc
    59461 atcccaaatc ctgaaggaac agcgcctggg atgtggtttg aaaaggatgg taaaatattt
    59521 atatctatgc cgggagtgcc gtttgagatg aagtcaatat ttataaatca aatagctcct
    59581 aaactggcaa aagttaataa taccctttac attttgcata aaacgatatt aacccatggt
    59641 gttggtgaat cttttttagc tcagactatt gaagcatggg aagataacct gccatcgaac
    59701 ataaaacttg cttatttgcc acagcccggc gttgtacgtc tgaggctttc agcagttgga
    59761 aataataaat cattactcga aaatcaatta aatgaggagc aggttaagtt gttgcatatt
    59821 atctctgatt tggtttttgg gtttgataat gatagtatgg aaggcgttgt tggccaactt
    59881 ttgaggatgt caggcaaaac tttaagtact gccgaaagct gtacaggtgg atatatagca
    59941 catctgatta ccagcatacc gggtagctct ttctatttca aaggatcagt agttgcttat
    60001 tcaaatgagg ttaaagtttc tcaattaaat gttgccagac aagcaataat agataatggc
    60061 gctgttagcg aagtggttgt taggcaaatg gccgagggtg ttcgtaaaaa tttacaaact
    60121 gactattcaa ttgccgtaac cggtgttgcc ggcccggatg gcggatcgga agagaaaccg
    60181 gttggaacaa cctggatagc agttgccact tctgaaaagg ttgtgtccca taggttccac
    60241 tttggcgaac accgtgatcg aaatatccga agagcagctc ttgccgctct taacatgttg
    60301 aggcaggaac taaaatgatg aagataattt tatattctca ttttggcatc tcaatgacct
    60361 ttatttccgt tcggcgattt ttagctcttc cttcctgagt ttggtttgtg gttaacggct
    60421 tggtaaaacc atatcctttg tgttgtagtc tgttgctatc aatgcctttg gcaaccaaaa
    60481 agtcagttac cgcctgagct cgttgatcag aaagggtcat attgtaatcc tcgcttccta
    60541 cgtcgtcagt atgcccgcta atttcaacag ctatagaagg attttgaata agaaattcgt
    60601 ataatttgtt taattcagct aacgactcat cctttaagtt tgctttaccg gaaccgaaaa
    60661 agatatttct cattattgta acttcgcccg ggattatagg gttcaaagcg atattcagca
    60721 gatatggctc gaaaactccc ttgtagtcgg tcagagaaaa atgctcactg aaaaacaaat
    60781 acccctccct tgatacattt aaagcataat ctctgtttat cggaaggcat agtaaaaatt
    60841 cgccggtttt ttcgtcagaa aatgatgtga taatatcttt ccctgcatct atctctgtca
    60901 atgtaaaata ggataacaac ggattattgg tttttgcatc aacgactatc ccttttacgt
    60961 aggtcgaagg cttaggtctt atttcgggat agacttcaaa ctcataaatg tcaaatccgc
    61021 cgtttccttc aggtagtctt gctgaaatat aggccttatc acctcttgca tttacaataa
    61081 tgttaatttc atcgcccgat gtgtttaatg gatatccaag gttaacaggt ttttcccaat
    61141 taccggcgct gtccattttt gccacaaaga ggtccatacc tcccatgcct gggtgtcggt
    61201 cggaagagaa gtaaagcgtc ttgccatcag catgaatata gggtgacatt tcggatccgt
    61261 aggtgtttat tgtctcccca aggttttccg gtgtgctcca ttcgccatta tcctgcagat
    61321 agcttctcca aatatcggat tttccatatc caccttttct ggtactggaa aaatatagcg
    61381 ttctgccatc cggagcaagg catggttgtg aatcccaggc tgtgctgttt aaggtatgac
    61441 ctaagttttg aggctcgcca atgtttttac caacagtttt aacagcatat atatcgcagc
    61501 ttccatagcc atcagaccac tggcagcctg caaaaagtag atacctgcca tcgtgcgtta
    61561 gactcagcgc cccttgagta tcctccgagt tgagcggtgg cggtagcaat gctattggtt
    61621 gccaagccga gttaagatca tcacgatagg aaatgaaaaa tctgtcggaa acgccgacca
    61681 aaccttgtac ggcctcttca gctgttatgt aaatcttaag ctcatctgta cttactgcgt
    61741 ttataaattc atcattggcg ctattaatat ttgttccaag gttaataggt tgaaaaggta
    61801 ccgggttttt tagcgatcgt tgtgcaaatc gggcagaggc aaggtaataa tcggcctctc
    61861 tctgccttgc ctcattgatt atgttatagt ttttaaactg ttcaagttgt tctatacata
    61921 attggtattg ctgcatctca aaacaaagaa taccataaaa atagaagact tcaggatatt
    61981 tatcagggtt gatttctatt gctgttttat atgtttctac agcatatatg tttttatttt
    62041 gtgttcggta gatatcgcca agaagcatgt aagcttcaag gaaattttta tcggtaccaa
    62101 tagctttttt cagttttagt tcagcctgtt cgtattccaa aagagcaaaa tgatttctcc
    62161 cctcatgata taacctgatt gcttttttcg atgtagttgt aagtgtttga gccaagctta
    62221 cataaatgct tgaaactaaa aaaataacca ccaaaagaga tctgataatt gatgctaatt
    62281 ttaaatacaa aaaagacata cgctttgagt ttatcaaaac aactaacgct gatgaagcga
    62341 tggttttttt gccgctgttt aaaaacgata ttgtagtgtt gtccattttt acttgttagg
    62401 aaagcctttt aaaggatttc atttctcgaa aagttataaa tccgtgtttc agagtttttt
    62461 ttaatgttgg aaaataattt ttgttttaca ggtcccactt ccaaataccg cacaaaagag
    62521 atgtcttaat ttgttatatt tcagtacgct tattattaaa tgagttttat ttacataact
    62581 aacattttta atgactatgg aatgttcatg aatttatgag attgtaatga gattttccat
    62641 ttcggatatt tttttatgta ctcgatgata actggtgtca taaattttga acggctccat
    62701 tcgggttgga ggtataattt acatttattg ctcactaaat tagcatattt ttcggcccac
    62761 gtcagatcgc cgtcattaaa aatgataact ttcagctcat ttgcaataga ggtaatactg
    62821 tcaattggag cgctgttttt tttaggagag aggcatatcc agtcccattg accggagaga
    62881 gggtaagcgc ctgatgtttc aagataagtt tttatagaat tttttttcag ctcggcgcaa
    62941 agatagttga ggttatacat taaaggttct ccaccagtaa ctaccacagc ttttgaaggg
    63001 taggcggcag cttgttccac tacctgaagc gtacttgtga gcggatgtat ttcagggttc
    63061 cacgattctt tgatatcgca ccagcggcag cctacatcac agcctccaat ccttatgaaa
    63121 aaagcggcct cgccagtgtt gtaaccttcg ccctgaagtg tataaaaagc ctccataaca
    63181 ggcaaaagct tgccctcttc aaaatgtctt ttctgttcgt aacttataaa ttttggattt
    63241 aacacaggta ggtaatactg ttttttttcg ttgaagaaaa tgatgcgaca ataaggtgtg
    63301 gatttaaatg actattttct gtttgcttta aatgctttca aggtattcat tagaagtgca
    63361 acacttgtca taggaccaac acctcccggt actggcgtaa tatagctgca tttagcggct
    63421 acttcgttgt aattgacatc gccggtaagg gaatatcctg atcgctttga ctgatctgct
    63481 attcggtgaa tacccacatc tattacaact gctccctctt ttaccatatc cgcggttaca
    63541 aattcgggct gcccaatagc tgctataaga atatcggcct gagtcgtatg ttctttgata
    63601 ttacgagttt gtgaatgaca aatagtaacg gtacaattac ccggatcgct ttttctcgat
    63661 agcataacac ttaacggagt ccctactatg ttactacgtc ctaatacaac acagtgtttg
    63721 ccggcggtat caatcttata tcttttcaaa agctctacaa tgccataagg cgtagcagga
    63781 aagaaactct cctgtccaag catcatttta ccaagattgt atgggtgaaa tccatctaca
    63841 tctttcttag gatcaatgga tccaattatt ttctcgacat taatatggcg tggaagtggt
    63901 aattgtacga taaatccatt aatttcttcg tcttgattaa ggaaattaat agtttctaaa
    63961 agttcagatt cagttgtacg ttcgctcaaa cgatacacgg atgacatcat gccaaccgat
    64021 ttgcaatctt tttccttact tgcaacgtat gtttcactgg caggatcatg accgaccaag
    64081 atagccgaga gatgtggaga ttcatcagta ttatcaatga tctgggcaac ctctgctttg
    64141 atctcctctt taattgctgc tgctacagct tttccgtcaa taatttgcat attatttttt
    64201 gttaaagaaa ttctttgtaa gatgtttaaa tactgaatga gtttagaata tttattcttt
    64261 ttaattcttt ataatgtaag ttctgcatat taagccattc attttgccag agtaactttt
    64321 aggttgaaaa tcatagcgac tcgatttttc tcctatttga tatttatctt tttgcccccc
    64381 tgcatatttt gcatcatttt catcatcccc tttccaccac ttgtagtcat cattttcatc
    64441 atacggcgtg tttcatcaaa ttgtttgatc aaacggttaa cctcctggat atccgtgccg
    64501 cttcctaagg ctattctttt acggcgactg ccgttgatga ttttaggatt atctctttcg
    64561 ctgggtgtca tggaataaat tatagcctct acatgtttaa atgcgtcgtc gtcaatatcg
    64621 aggtttttta ttgctttgcc cattcctggt atcatactca taagatcttt tacgttaccc
    64681 atttttttga tttgctttat ctgcgcaagg aaatcattaa agttaaactg atctttagca
    64741 atttttttct gtagtttcct ggcttcctgt tcatcgaatt gctcttgagc tttttctact
    64801 aacgatacga tgtctcccat ccctaagatt ctgtcggcca tacgttttgg gtagaagacg
    64861 tctaatgcgt caattttttc gccgattcca acaaatttta tgggtttact aactacagct
    64921 ttaattgtta aagcggctcc accccgtgtg tcgccatcta attttgtaag aacaacacct
    64981 tcgaagttta gccggtcgtg aaatgctttg gcggtgttca cggcgtcttg tcctgtcatg
    65041 gcatcaacga cgaaaagagt ctcctgtggt tttaaagcct cttttataga gctaatttca
    65101 tccatcattt gctcatcaat agcaaggcgg ccggcagtat ccacaatcac tacattatat
    65161 ccgtttgctt ttgcatggtt gatagagtta cgggtaattt ttacaggttc catactgccc
    65221 tcttcagtat agacttctac atctattgta ttacccaata cctgcaactg attaatggca
    65281 gccggacgat agacgtcgcc ggcaacaagt aaggggcgtt tgcctttttt ctgttttaaa
    65341 taattagcaa gttttgctga aaaagttgtt ttccccgagc cttgtaagcc ggcaattaaa
    65401 attattgcag ggtcgccttt aatattgatg tcggtatgtt cgcctcccat cagctctgtc
    65461 aggttgtcgt gtacaatttt aatcattaat tgacccggcg aaatagctgt caagacattt
    65521 tgacccaaag ctctctcttt aacttcatca gtgaattgct tagctatttt gtaacttaca
    65581 tcggcatcaa gtaatgcttt acgcacctct ttcaaggtct cggaaacgtt aatttcagta
    65641 atctttccct gacctttcaa tattttaaac gacctgtcta acttctcact taagctttcg
    65701 aacatacacc ttaaattaat ataataccaa aaaaatcttt tttgttagat gacaaaaata
    65761 ggttaatttg aaaatataaa cccgaattaa tcaataggaa gtctatttaa gttgaactat
    65821 caattttatt aaaccaacct taatttagtg tcattcagaa caatactcac tgtgttgatt
    65881 gcatgcatta aattatttaa atattgtaaa ctgattgttt aaagataaat cttacctttg
    65941 caaagataaa aaaaattaat tttttatttg ggagttttta aatgttaaa
//
LOCUS       DAOOAI010000002        56239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_679_length_56239_cov_5.150452, whole genome shotgun sequence.
ACCESSION   DAOOAI010000002 DAOOAI010000000
VERSION     DAOOAI010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 56239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 56239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..56239
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_679_length_56239_cov_5.150452"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="PK345_00250"
     CDS             <1..597
                     /locus_tag="PK345_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33482.1"
                     /translation="HPGVNPVGEFIIDTSCNLYNFQSRPNTETKPSMVKTYDNNFVIN
                     ANIKVGNYFKLYLYKIDSNLESVPLDTTQYVYDSLCPHTIQSGTIDLTDCLVIQSIDE
                     LPSPDEYYESIRWIPVKAYPNPVSGQELTIEFENTQHHSNIELRLYDAFGKEVHRRRI
                     YTGQQDISLDVGHWPPGLYLVVVYSNGGAVGQCKVVVE"
     assembly_gap    961..970
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<971..2444)
                     /locus_tag="PK345_00255"
     CDS             complement(<971..2444)
                     /locus_tag="PK345_00255"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPX33483.1"
                     /translation="MKRKLQTYSLVIFALIGLNFSFSLFAQSEYQSKLSILDEYYAKA
                     LSDWKVPGMAIAIVKDGEIIYAKGFGVKDVRTKEPVDSETLFPIASNTKAFTSAALAI
                     LVDRGLISWSDKVTDYLPYFKLWAPYVTENITISDLLTHRAGLATFSGDLLWYGTLYS
                     REEIIKRARHLKPAFGFRERFGYSNILYIAAGEIIPAVTGKSWEEFIKEELLESLKMN
                     RTVLSTNDLPGKKNVATPHTDYNEDVIPIEYLNWDNMGPAGSLISCVNDVAKWLVLQL
                     NRGVIDGDTIFSSERSREMWTSYTQMPISSFLETNMPSTTFRSYGLGWGLWNYLGKKI
                     VEHNGGYDGMISQTCMAPEENLAFVILTNKNSNLYYPLMYKTLDTFLGGEEKDWSMLL
                     LDRVKKQKEAEEKRREQKNKERIKNTDTSLPLNCYTGTYGGELYGNAMVKLNGEKLTV
                     HMQPAPIFEGTLKHWHFDTFEITFENIPSLPSGFCTFILDN"
     gene            complement(2594..3433)
                     /locus_tag="PK345_00260"
     CDS             complement(2594..3433)
                     /locus_tag="PK345_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPX33484.1"
                     /translation="MKKYFVLLVVLLLSNCHFAIAQKDIPDRPSPPKLVNDFTGILSS
                     AETHTLEQKLVTFNNETSNQIAIVIVNDFAGYQKEQYADLIGEKWGVGYKGKNNGVVL
                     LVKPKTETEKGETFIATGYGLEGALPDAVLKRLVEEEMIPFFKTDRYYQGLDHTTDII
                     MKLVAGEFTADEYLGNRDVLKGLFFVFLILVLFIAFVYRKLKMANVKVKNYSSGHHGI
                     SAMQLLMFLLEADKGREKGKWGSFNSGGGLFGGFGGSGGGGGGFGGFGGGSFGGGGAG
                     GSW"
     gene            complement(3446..3913)
                     /locus_tag="PK345_00265"
     CDS             complement(3446..3913)
                     /locus_tag="PK345_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HPX33485.1"
                     /translation="MEADSKKGNVGLSSEDEKSVRQAIIDAELKTSGEIRVHIEENCR
                     VDVLDRAAYVFEKLKMHKTKFRNGALFYLSMNDHKFAVIGDAGINAVVEDNFWEEVRD
                     KLAVYFANGQFADGLVHGIKLTGERLKRYFPYPDDDINELPDDISYEKFPKTD"
     gene            complement(3931..4527)
                     /locus_tag="PK345_00270"
     CDS             complement(3931..4527)
                     /locus_tag="PK345_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HPX33486.1"
                     /translation="MKNRRLYVTLGVIALVVILIFSGAKNTYNSMVKYDEGVNVAWSQ
                     VVTDYQRRMDLIPNLVNTVKGYADFEQETLIGVIEARSNASKVNLNVDNLDENSLRMI
                     EQTQAALSSALSRLLVTVERYPDLKANTNFRDLQAQLEGTENRIANARRKFNEATQEY
                     NFYIRQFPKNIYASIFGFRTKPYFEAAEGAEKVPEVQF"
     gene            complement(4606..6510)
                     /gene="htpG"
                     /locus_tag="PK345_00275"
     CDS             complement(4606..6510)
                     /gene="htpG"
                     /locus_tag="PK345_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HPX33487.1"
                     /translation="MQKGKIKVQTENIFPIIKKFLYSDHEIFLRELISNAVDATQKLK
                     TLASTGHFKGELGDLTIHVSIDKKRGTLTVKDRGIGMTAEEVDKYINQIAFSSAEEFV
                     KKFKTKTEAEQNAIIGHFGLGFYSSFMVSDKVELKSKTYKKGGPTKAVKWTCDGSPDY
                     TLEEDDKADRGTEVTLFISKEEKEFLEDYKIRELLKKYCSFLPVPIQFGTKKEHVKVE
                     GEKDKDGNDKYVEVEKPNIINNTDPLWKKAPVDLKDEDYKDFYRELYPMNFEEPLFHI
                     HLNVDYPFNLTGILYFPKVKRNFEVQRDKIQLYVNQVFVTDSVEGIVPDFLTLLHGVL
                     DSPDIPLNVSRSYLQSDRRVKQISNHIMKKVSDKLEDIFKNQREEFEKKWDDIKVFIE
                     YGMISEEKFYDRAKKFCLLKNIEDKYFTFDDYKEKIKGTQTDKDKKLVYLYATDKETQ
                     HSYIESATERGYDVLMMDGVLDNHFVNTLEHKFENSSFVRVDADTIDKLIKISDDENI
                     SKLDDKQKELLKPIFEKAVDKSKFTIVFDNLSETDMPVMITQPEFMRRMKDMSALGGG
                     YSGLDAFPDRYNLVVNSNHNLVYKMANEENDESREKMAQQLTDLALLSQNLLKGEELT
                     KFIKRSVEIL"
     gene            complement(6972..7565)
                     /locus_tag="PK345_00280"
     CDS             complement(6972..7565)
                     /locus_tag="PK345_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33488.1"
                     /translation="MKRYPLLSFGALLLLAFVATPLFHSCKSSKKAVKVASGEEKIIV
                     YCSGPEYFTDNKYFRANDVAESANQSMAKRKAMSNARLALASSIEITVKAVIDNYFNS
                     YAVDKTIEDKERYEGLSREVVNQKLNGVRIICEEYTKTTDGNYKCYVAIELAGDEIAN
                     GVIKRISDDEKLKLDFQYEKFKETFEKEMEEFKSQRY"
     gene            complement(7788..8621)
                     /gene="tsf"
                     /locus_tag="PK345_00285"
     CDS             complement(7788..8621)
                     /gene="tsf"
                     /locus_tag="PK345_00285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HPX33489.1"
                     /translation="MSNIKAADVAKLRKQTGAGMMDCKQALTESNGDFEKAIEILRKK
                     GAKLAAKRADKDATEGYIMAQTNEENSFGVLVMINCETDFVGKSEEFVNFVKTVASLA
                     LKNKATSLEAVRGLKIEDRTVEQALSDLIGKTGEKMEFDSYEFIEAPFVSSYNHLGNK
                     IGTLVGLNLKTADVAVKGHDMAMQVAAMNPVAIDKGDVTKEMIDKEIEIGMELARQEG
                     KPENMLEKIAQGRLQKFFKESTLLNQEFIKDSKKTVGNYLKESDKNLTVVKFYRFGLG
                     A"
     gene            complement(8653..9558)
                     /gene="rpsB"
                     /locus_tag="PK345_00290"
     CDS             complement(8653..9558)
                     /gene="rpsB"
                     /locus_tag="PK345_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HPX33490.1"
                     /translation="MARTNFEELLNAGVHFGHLKRKWNPNMAPYIFMERNGIHIIDLY
                     KTAAKIEEAASALKQIAKSGKKILFVATKKQAKDIVAEKVKSVGMPYVTERWPGGMLT
                     NFATIRKAVRKMSSIDTMMKDPTFTNISKRERLQITRERAKLEKQLGSIADLNRLPSA
                     LFIVDIMKEHIAVAESRKLNIPTFAMVDTNSDPKLVDFPIPANDDASKSIALIVEIMV
                     RAIEEGMMERKVEKDKQFKEEDEGIESIKTRTRQELEAELEEDKDEDERTIKKEEIRK
                     LKKTEEEETGQKEKRARKGTAIRKK"
     gene            complement(9664..10050)
                     /gene="rpsI"
                     /locus_tag="PK345_00295"
     CDS             complement(9664..10050)
                     /gene="rpsI"
                     /locus_tag="PK345_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013545814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HPX33491.1"
                     /translation="MEIINAVGRRKTAVARAFVQTGNGKITINKRDFENYFPVESLQQ
                     IVRKPLELTDNVDKFDIHVNIDGGGITGQAEALRLAISRAIIKIDPEHRAILKSNGFL
                     MRDPRMVERKKPGQKKARKKFQFSKR"
     gene            complement(10053..10508)
                     /gene="rplM"
                     /locus_tag="PK345_00300"
     CDS             complement(10053..10508)
                     /gene="rplM"
                     /locus_tag="PK345_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008656690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HPX33492.1"
                     /translation="MDTISYKTISANKETVTKDWVLIDAENEVLGRLASKAALLLRGK
                     LKPNYTPHVDCGDNVVIINADKVRLTGSKWADKEYITHSGYPGGQKVLTAKQMKLRKP
                     TFIVENAIRGMLPKNRLASQLFRNLYVYAGPEHKQEAQKPKKINLNEIK"
     gene            complement(10793..13432)
                     /locus_tag="PK345_00305"
     CDS             complement(10793..13432)
                     /locus_tag="PK345_00305"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005847276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HPX33493.1"
                     /translation="MMIPSKYDPTLVEDKWYRYWLDKGYFRSVPDDREPYCIVIPPPN
                     VTGVLHMGHILNNTLQDVLVRRARMLGKNALWLPGTDHASIATEAKVVEKLKKEGIDK
                     KDLTREEFLSHAWEWKEKHGGIILEQLKKLGASCDWERTKFTMDEDLYESVIDVFIDL
                     YNKGLIYRGVRMVNWDPQALTALSDEEVIYREEDSKLYYVRYEIDNTDDEWVTIATTR
                     PETILGDTAVCVNPKDKRFKNLKGKKVFVPLINRLVPIIQDDYVDMEFGTGCLKITPA
                     HDLADYEIGLRHKLETIDILNDNGTLSPAAQLYVGEDRMVARKKIVDDLKNAGHLVKI
                     EDYKNKVGYSERTNIAIEPKLSLQWFLKMPELAEPALKVVMDDVIEFHPKKFKNIYRH
                     WMENVRDWCISRQLWWGQRIPVYYLPNNEMVVAKSKEEAITIAKSQFPELADLTQEEL
                     KQDEDVLDTWFSSWLWPISVFDGIRNPDNPDIKYYYPTNDLITAPEILFFWVARMIMA
                     GLEYRGEIPFKNVYLTGIVRDKLGRKMSKSLGNSPDPIKLMNHYGADGTRVGMLLTSP
                     AGNDLPFDENLCEQGRNFANKIWNALRLLKGWKVDDNLPQPDTNQVAIEWFNATFNKT
                     LGLIDDHFAKYRISDALMVTYKLIWDDFCSWFLEMIKPGSDNPVDRKTYDDTITLFEG
                     LMKVLHPFMPFLTEEIWHLLCERTQDDCIMISEWPKKGRINHLILNQFEFAKEVIIAL
                     RAVRAEKNIAMRQTLDLYIKKNLDQKPDSTFDGIVAKLCNLTKIEYVNEKVDKAMSFV
                     VKTTEFYLPLGDVVNVEQEIQKLEDELNYTKGFLAAVLKKLGNDRFVSGAPLAVVEKE
                     RQKQADAENKIKILEDQIAALKL"
     gene            complement(13554..14168)
                     /locus_tag="PK345_00310"
     CDS             complement(13554..14168)
                     /locus_tag="PK345_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HPX33494.1"
                     /translation="MLSDDTYKTVTKRSQGLYKDKGSRFISFAIPVKSEEEIRTELEL
                     LRNQYHDARHQCYAWILGYNKETYRYNDDGEPSGTAGKPIYGQLLSNDLTNTVIVVIR
                     YFGGIKLGVRGLINAYKFAAQDALANNVIQTRIIHDIYQVKFAYEHMNHVMRILKEEE
                     LDQIDQDFHLTCSIDFSVRSSRADYVVTRFNDLRMVELTFLRRE"
     gene            14629..14979
                     /locus_tag="PK345_00315"
     CDS             14629..14979
                     /locus_tag="PK345_00315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33495.1"
                     /translation="MEVELSTAVSIAEHQSLVFGKKDLRIKIFPIIFHLTSDYPFEGM
                     PEVSFVEDFTRMAKMKITNFHGQEVANVTLATIPGRNRINLYAHFVDGSKEDLEQIMS
                     FVATVTLESKLSGF"
     gene            15374..16495
                     /locus_tag="PK345_00320"
     CDS             15374..16495
                     /locus_tag="PK345_00320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33496.1"
                     /translation="MKSRIIFSAMLIAFASITMFSCKKDKKDSPKELTPEDAKIELND
                     ASQQFDNQIVEIMQTESVQTANFLLELLGIDLEFDLYKNLLEQPGNIHLTKIKNALKA
                     ANSTNSSNELEEYGIYEFDFDSEEFIMVEPTDASIIFYFPADETAYMNMQNNAEIAMT
                     NIQTTIVLYTEEYWDWELEQWVTETYEEEVPTNCKITEKVDGATLATINYNSTITENG
                     FPTAFNVSADVPPYNINTTLNGSGTKFQTQLSFKKNSTSLFSFDLTIEYTSDMENIKK
                     INGNLTPNPLKIEGFINIFAIETHMYEHEFSGDWDIDYLNGQIDLELIQVEKNAKIGD
                     IEYKSYYDYPALAVVYSDGTFDWLENVINIGFKHRRTRK"
     gene            16842..22325
                     /locus_tag="PK345_00325"
     CDS             16842..22325
                     /locus_tag="PK345_00325"
                     /inference="COORDINATES: protein motif:HMM:NF019323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MG2 domain-containing protein"
                     /protein_id="HPX33497.1"
                     /translation="MLLSCKHGATPFQMSDPADYDKIIVGHTAGIISSQSAVQVTFAK
                     AIESYQPGAELPADVFTFVPSISGTATVEDSYSISFKPNKPLKQGIKYAVSLNLVRLM
                     EVPKELKKFNFGFEVITQDFTVLEGHLMPESIDIADKYLYKGIIITADLMSVEEVEKL
                     LKASAQNKDLNIQIESEGIKKFSYTIRDIERLDIGSKVTLSWNGDKIGLKKRGDLDIV
                     IPGKNEFILLSHEVNHENKSLQLIFSDPIDNKQEIAGLIGFQSTDDVRISRYGAIVTL
                     FPNQTLIDEQVVKISEWLRSAGGITLGKPITLFVQMDALKPQVEIIGNGNIIPDSKNL
                     FLHFKAVGLKAVDVAVYKIFANNIKQYFQEFTLENSGELRYVGRPVFIETVRLDKDDK
                     TDLNRWNAFSVDLTEMVRKDTEAIYHVKISFKRNYSLFNCNNELTDDNEIEQAVITEE
                     EVDYFDGKSYWYYYWPENYNWYEQDDPCSDSYYINDRFPTRNILASNIGILTKSVDNL
                     KFSVAITDLLTTKPIDGSLVEFFNFQQQKIGEVSTSASGMAEIELTSSPYLISASYQN
                     QKSWLKVDNGSSLSLSNFDVSGKEIQKGIKGFIYEERGVWRPGDTLFITFLMDDRLQK
                     LPNNHPVLFELFDSRGNLYKKQIKTEGVDNFYTFITPTKPDAPTGKWLIKISVGGAIF
                     EKRINIENVKPNRLKMDIRFDDKVLTTNSLHKPGFLKSSWLHGTAASNLKATVNLKLC
                     PIQYTFKGHDKYQFTDPTKTFNPIENIIFDAPLDSNGEAPISLQSMSNIHSPGMLNAV
                     FTTRVFEYGGDFSTDVFEIPFSPFDVYTGINITGADGIEDVLETDKDIAVEVVTIDHK
                     GNAVSQDNLELNIYKLSWRWWWSSTNDNIASWVNGQGTELIEHKTFYTTQGKARQHFK
                     IEYPEWGQYFVQVVNTNNRHSTGKIVFVDWPNSFNRSGRKHPAAATMLSFTTDKEKYT
                     TGENATVTFPSTEGANAMISLETSSGIIKQWWQECEKDKTSFDIRITPEMTPNFFVSI
                     TLIQPHGSTVNDLPIRLYGVIPVMVEEPQTRLTPEIRVEKEIRPEAPFTIKVSEKSGK
                     AMTYTLAIVDEGLLGLTRFKTPDPWSSFFAREALGVKTWDMFDQVMGAYGGELQKVLA
                     IGGDDELARAGEKKANRFKPVVIFSGPFTLKAGKQGLHQFTIPNYTGEVRIMVIAGKN
                     GSWGASEKNVNVKQPIMLLPTLPRKLSPDEEVIMPVSVFSMSNKVKKVAVKIKTDGHL
                     IPLGNTYQEADFSDIGEKMVYFSLKADGSEGYSSVSLEATSGSEKATASVEIEVKHPN
                     PYTTISESHIIQEGAQQKIDITPHGLPQTNSVKLNISSLPSFEIEKSLQFLINYPYGC
                     LEQIVSSSFAQLYLSDLIELSDNQKVTTDRNIRATLKALTGYNKGGGRLSYWPGSATS
                     STWSEVYAGHFMFLAEQKGYSLPANLKSSWLEAQSRLAMQYNSAAEQENKHDINTQAY
                     RLYVLALAGKPVFSAMNRLNETNNITKEAAWRLAAAYYLAGRPETAEKLIKQTDKLQT
                     TEHYFMDENFGSSLRDQAMIVETMTLMNKKQEAFNQLNRMARQYNAENASTQTAAFCL
                     YALSRYAKLTGSEANLNFDLTVNNKKESLNSTSPVYEKDLGKFDNPSSITIQNKGKGD
                     LITTISVTGQSPYSTEQTTASNLRMQTRFLSTDGRLINVGSIKQGDDFTAEISITHPG
                     TGGDYKNMALNFAVPSGWEILNRRFQNTEVVVMESKYTFRDIRDDRVDTFFDLPAGKT
                     VIYRIELNASYEGKYYMPAVKCEAMYERSIYAIEKGKWIEVKK"
     gene            22560..24878
                     /locus_tag="PK345_00330"
     CDS             22560..24878
                     /locus_tag="PK345_00330"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33498.1"
                     /translation="MKKFTWILLLVLVSGFSHAQSQNVPEAGKFGGAEKQVVYKNQAG
                     YSLQHMINNGHYQMRALIDYDSLNMSFLGNWPSGINYSLIYSGIDNIFMLGSGGTMIV
                     LDLSDPEQPETISEVRSRSLVDCAFFDTETNRLYLGAYFSGVEIWDLNDMQHPFRLAR
                     IPTESYPRGGVFAEGNIVYVMTVAAGIYIYDIENLEDINLIGHYPIPSSSLVWNSAKD
                     GDLMFCAAGNACRIVDVSDPSNLVMAGVIAGASAGVYAENGKFYQLSSTSGLKIWDVS
                     NPATPQLLGQIAISGNPAKISVNGDYAYITSAANEPNGGLKIIDVSDPTNPQEKSSFV
                     CYTEHVATNGSLTLISAGSECKVIDVTDINSPQLKSTISLPGWVHNVFLKGNYGYTGS
                     NGFRVFDLSDKSNPVQVGFNETLGAFVATNSDYAVYIPYSAGGNNKVNIMDISDPTSP
                     KKMGHYVSPAMTYKVDLKDNYAFIACWWDGVRVINFSNPESPVLAAHVLGWYNGAIPG
                     DEYCFAQSIDVVGDYLYVIDYMPFEDQDTKGIYIFNISDPANPVFINRFKEFNSKCND
                     ISVQDDYAYLADNVGGVEVINIADPNNPFVVGYVYLPDVAEAITTEGKYAYVSNYILG
                     GVQMLDISFPPNSNIAGYYKRSGVFALGVSVQGSHVYIGDGIAGFQIYDNLFITSVED
                     LNKNNSEQLDIYPNPALQSAKANFITTTNSYCNLSIYDLTGRKVQEIFNGWLDAGEQT
                     FEIKSNQLKAGIYFVKLEINDQVRTSKLVISH"
     gene            25044..26570
                     /locus_tag="PK345_00335"
     CDS             25044..26570
                     /locus_tag="PK345_00335"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPX33499.1"
                     /translation="MKKYFILASCLLFLNVILAENPRFIQAQLSYTTFYSPADGPYIE
                     TYLSVIGGSLEWVQNDLGQFQSKLNVTMIFKDGGKIAAFDKYELSSPIISDSAERFIT
                     FLDQQRFSLPNGEYDFEITLKDPNADTPPFSANETIIINIPKDAVSISGIQLLESYKK
                     SEEPTVLTKSGYDMIPYIYNFIPKEVNTINVYAEIYNTDKVFGTDQKFLLSHYIQSYE
                     TKRTMSSFSHFRREESKPVNVLLVNYNIAELPSGNYNLVIEVRDRENMLVNSQKLFFQ
                     RSNPDLKIEQPIYASISIENSFVNKITNQDTLTEYIKFLTPIATEIENRFIRQQIESQ
                     NADLQLMQRFFLNFWLERDEANPELAWNTYLAKVNLVNEQFGSPSKKGKRGYQTDMGY
                     VFLKYGPPNTITDRPFDASSSGLTINPGGEETIAMGSVPYQIWHYYSINEYRDRKFVF
                     ANHHLALFDYQLIHSNMPGEVQNDNWQSELFYRFNVGGTMPETDKYKGQSGDFYNHPR
                     "
     gene            26692..27705
                     /locus_tag="PK345_00340"
     CDS             26692..27705
                     /locus_tag="PK345_00340"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPX33500.1"
                     /translation="MNISIVILNWNGKKYLGTFLPKLITSSPEANIYVADNGSSDGSV
                     RFLQQNYPEIKVIPFTENHGFAKGYNLALNQIEADYYILLNTDIEVTDNWIQPVISVM
                     EQDESIAACQPKILSYDNRRHFEYAGAAGGFIDRYGYPFCRGRIFQVIEEDVGQYDDI
                     TEIFWASGACLFIKSNLFKKAGGFDNDFFAHMEEIDLCWRLKHLGYKIMYCGQSTVFH
                     IGGGTLDKSAPKKTYLNIRNNNILLYKNLPSKQIIPTFIARFFLDLTASLKFLIDGGV
                     RHFIAVSRAHIGFYFSLRKNHLKRKKIKHIKVSKIYLGNIAFDHFLFGKKTFKQLKSK
                     MFT"
     gene            28027..29820
                     /gene="rho"
                     /locus_tag="PK345_00345"
     CDS             28027..29820
                     /gene="rho"
                     /locus_tag="PK345_00345"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HPX33501.1"
                     /translation="MYDIIELNAKLLPDLREIAKELNITKINNLKKQDLIYKILDQQA
                     INPPKSDTSKKSDPFFDKNQRRRLQSSLIKEKIASSSDSSPSVAVSGVTHITPEAKPV
                     TSLSEEKKSQKQFPHDKPRKQKTDHKVQKAEQENRFNKSSATDKQSSPDDDTVDQKLE
                     ETLIVQKRTHVLNKTKDLYKKPEVVVTNLADDQKTTEAKQSQVATTAEQQRHSVNDRQ
                     REKFSFEIDGIVDTEGVLEIIPEGYGFLRSSDYNYLNSPDDVYVSQSQIKLFGLKTGD
                     TLSGSVRPPKEGEKYFPLIKIERINGRDPNEIRDRIPFDYLTPLFPEKKFKLTGHPQE
                     TISTRIIDMFTPIGKGQRGLIVAQPKTGKTVLLKDIANAIAYNHPEVYMMILLIDERP
                     EEVTDMQRSVRAEVIASTFDEPAERHVRIANIVLEKAKRLTECGHDVVILLDSITRLA
                     RAYNTVAPASGKVLTGGVDSNALQKPKRFFGAARQIEHGGSLTIISTALIDTGSRMDE
                     VIFEEFKGTGNMELQLDRKISNKRIYPAIDIVASSTRRDDLLLDKEMLQRVWILRNIL
                     SERTTVEAMEFLKDKMRFTQTNEEFLISMNS"
     gene            29900..30088
                     /gene="rpmG"
                     /locus_tag="PK345_00350"
     CDS             29900..30088
                     /gene="rpmG"
                     /locus_tag="PK345_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HPX33502.1"
                     /translation="MSKKTKDVRIQVILECTEHKNSGLPGTSRYVTMKSKKNTPERLE
                     LKKYNPIMKKVTVHREIK"
     gene            30104..30259
                     /locus_tag="PK345_00355"
     CDS             30104..30259
                     /locus_tag="PK345_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4295 domain-containing protein"
                     /protein_id="HPX33503.1"
                     /translation="MAKKVVATLKTATGRDFTKVISVVRSAKTGAYTFKEEMMPTERA
                     QELIKKR"
     gene            30340..31296
                     /gene="ftsY"
                     /locus_tag="PK345_00360"
     CDS             30340..31296
                     /gene="ftsY"
                     /locus_tag="PK345_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HPX33504.1"
                     /translation="MGLFDFFSKKKKEQLNEGLEMTKKSVFSKITKAIAGKSKVDDEV
                     LDNLEEILVTSDVGVTTTLKIIERIEARVARDKYLGADELNSILKEEIAALLSENDSD
                     SISGFDFPKMEVPYVIMVVGVNGVGKTTTIGKLAYQFKMAGKSVLLGAADTFRAAAVD
                     QLTIWSERVDVPLISQGMGADPASVAYDTLKSAVANKADVVIIDTAGRLHNKINLMNE
                     LTKIKNVMKKIIPEAPQEILLILDASTGQNAIEQAKQFTAATEVNALALTKLDGTAKG
                     GVVIGISDQFKIPVKYIGIGEKKEDLQLFDKFEFVDSLFSQE"
     gene            31309..32613
                     /gene="rimO"
                     /locus_tag="PK345_00365"
     CDS             31309..32613
                     /gene="rimO"
                     /locus_tag="PK345_00365"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005852485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HPX33505.1"
                     /translation="MKTKNGKPSVTIVTLGCSKNLVDSEVLIRQLQAGGIEVSHNQQN
                     NISKRVIINTCGFINDAKEESIDTILNFVNAKQQGIVSELYVMGCLSERYRHELSKEI
                     PEVDGFFGVSELQNILRSVGADYRKELLGERHLTTPNHYAYLKISEGCDRKCSFCAIP
                     LIRGKHQSKTIEEILNEAKYLASVGVKELILIAQDTTYYGLDIYGKRRLPDLLQQLTL
                     INGIEWIRLHYAYPNAFPEKTLSVINSSPKICKYLDIPIQHISDPILKAMKRSHSNGY
                     TRKLIEKIRNQIPGVALRTSIIVGFPGETNEQFDELKNFISEVKFDRLGVFTYSHEEN
                     TKAYKLKDDISAATKRKRASELMALQQQISFDINRKKIGTSLKVIIDRKDGDFWIGRS
                     EFDSPEVDNEILIPSTFPLKEGSLVKVKITDAEAFDLFAMPV"
     gene            32682..33449
                     /locus_tag="PK345_00370"
     CDS             32682..33449
                     /locus_tag="PK345_00370"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HPX33506.1"
                     /translation="MKNLMILFSVMWLSVSCSFSQEHKNQPTENINQTDRKGQKQGYW
                     EETEDKIKSVGIYANGKKEGSWLTYHPSGIVQLVESYKNGKKDGAFIEIDSRGYFTEE
                     QFYSNDKKHGKHRVYERGSRLVLEENYNNDQLDGTKKVFYSNGTLQEESEYKNGQKNG
                     TSKWFNQSGQIIAEYNYVNAKFEGTQKTFYENGVLMSEETFKENLRNGSAKEYHRNGK
                     LMKEGEYKDNLKEGKWLEYDENGNLINEIEYVKDEQK"
     gene            33436..33942
                     /locus_tag="PK345_00375"
     CDS             33436..33942
                     /locus_tag="PK345_00375"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPX33507.1"
                     /translation="MNKNNEQVFRKIRNPFTIHKGFNCFGCSPDNPIGLQLNFIDHGD
                     FITANWQPQTNYQGYHNLLHGGIQATLLDEIASWFVNVKLKTAGVTSKIEIKYKKPVY
                     VDSGPIMLKATLIRLMRNIADIKVELFNDEKQLCAVGVAQYFTFSEKIAKEKYYYPEP
                     NKFYFEKD"
     gene            complement(33956..35074)
                     /locus_tag="PK345_00380"
     CDS             complement(33956..35074)
                     /locus_tag="PK345_00380"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HPX33508.1"
                     /translation="MKKNRIIILILPLILTLNSCHVARYFYWNLADFDDYKKFPAETI
                     NKGDTAFHFIPSDSFISLNLPEGFCEGGFENSFIDFLENTKTVALLIIKNDTLQFEHY
                     SKGFQADAMVSSFSVSKSFVSALVGIIIEEGYIKSVHQSVTGFIPELLNSDIRFSKIT
                     LEHLLNMQSGIRFNEGYSTPFSDMAKYYYGKNLKKYVGRLKIKSAPGLEYDYVSVNSL
                     LLGIVLERVTGGNLAELIEQRLWRPLGMEYDATWSLDSKKRGQIKAFCCLNSRALDMA
                     KFGRLYLNRGNWNGLQVVPKRWIEATMKADGNFCDSQGIPYSYGWRVKEDGAFFAKGI
                     LGQYIYVFPDKEVIIVRLGKRNSGVDWVSFFEKLCSQL"
     gene            35523..36515
                     /locus_tag="PK345_00385"
     CDS             35523..36515
                     /locus_tag="PK345_00385"
                     /inference="COORDINATES: protein motif:HMM:NF005741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HPX33509.1"
                     /translation="MADLSTNYMGFKLKNPIIAGSTGLSMSVENLKSLEKHGAAAVVL
                     KSLFEEQIMHEVNKALAGTDNKDISYYPEAEDYIRHYTKEKDLNDYLRLIENGKKSIS
                     IPLIASINCFSASEWTTFAKKIESAGADGLELNIFILPSDTNREPSQNEQVYFDVVTE
                     VLKQVSIPIALKISYYFSGLAKTALKLSWTGISGMVLFNRFFSPDIDINKFIVTPSHV
                     FSTKDEIYTTLRWIAMLSNRLHCDIAGSTGVHDGEGAVKLLLAGAKAVQIATILYKNG
                     FETIGEMIRFIEQWMNKHDFKTTEEFIGKLSMKSTENPAAYERVQFMKHFAGIY"
     gene            complement(36518..37126)
                     /locus_tag="PK345_00390"
     CDS             complement(36518..37126)
                     /locus_tag="PK345_00390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33510.1"
                     /translation="METTNYYNDDSKLIIPSLFYDSISNMPIERCQVCQKKIIETDAE
                     YMIEKVFRRNPISGKMEVMFEFAICFSCALTFVNSYSAQSKENLQRYFAEHLEGRITH
                     IFQKSITSEIDIYDHLSTCAITGKKVTELEEYQIIGRFRGGFLKLDEPPFLIGANSMD
                     DVADLLSNETIDNMDDFTGKYLTGPPEFRDFFKSPRRRPVLI"
     gene            37336..38217
                     /locus_tag="PK345_00395"
     CDS             37336..38217
                     /locus_tag="PK345_00395"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009227242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA];
                     GO_process: GO:0050992 - dimethylallyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HPX33511.1"
                     /translation="MIIEIDPGAGFCFGVKRAISLAEDKLQQGGKLFSLGQIVHNKDE
                     EQRLNQLGMTTITHDQMDEVKDNPILIRAHGEPKSTYQKALKNNIELIDATCPIVLKL
                     QNEIRKCWLEGDEDNRQIVVFGKKGHPEVIGLQGNIDNQAIIIENISDIEKIDFSRPI
                     FLFSQTTADEELFDIIKNDIAKRLNDAQKSSTPPLHVTKSICSQVKNRKNQVITFASK
                     HDLIIFVGGKNSSNGRYLFSLCKAANTNSFFIENETNINPGWLTGVKSIGITGATSTP
                     DWLLEKVKSHINTLIQR"
     gene            38217..39332
                     /gene="recF"
                     /locus_tag="PK345_00400"
     CDS             38217..39332
                     /gene="recF"
                     /locus_tag="PK345_00400"
                     /inference="COORDINATES: protein motif:HMM:NF000823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HPX33512.1"
                     /translation="MYIKKLDIHNFKNYEKASLSFSPKVNCFIGDNAAGKTNLLDAIY
                     YLSFCKSYFNAVDTQNIRHDQEYFSILGEMDTDTNKTDLIHCVQKRNQKKSFKLNKKE
                     YQRLADHIGKYPIVMISPYDRDLINEGSDVRRRFIDSIISQFNSLYLDNLINYNKALA
                     QRNSQLRAFSEQNYFDSSLLEIWDHQLIDLGNQIFPERQKFITEYQPLFNKYFEVVTG
                     KKEAATIEYESQLNTHSFEELLASTIERDKALRFTTAGTHKDDLIFNIQGHAIKKFGS
                     QGQQKSFIVALRLAQYMYIKELKGYKPMILLDDIFDKLDNKRVEQLIQLVAENAFGQV
                     FITDTQQDRIEYLLNKISNNYRIFEVCNGTVNEIITL"
     gene            39345..39641
                     /locus_tag="PK345_00405"
     CDS             39345..39641
                     /locus_tag="PK345_00405"
                     /inference="COORDINATES: protein motif:HMM:NF017103.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF721 domain-containing protein"
                     /protein_id="HPX33513.1"
                     /translation="MSFRVPNEQTLGTVIRELLVTLQIEDKLKETQMIASWEEIVGKM
                     IAKHTKSLRIVKRVLYVEVDSAALRNELTYLREKIKKKLNKKAGDNLIDEVIIR"
     gene            complement(39763..41811)
                     /gene="ligA"
                     /locus_tag="PK345_00410"
     CDS             complement(39763..41811)
                     /gene="ligA"
                     /locus_tag="PK345_00410"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HPX33514.1"
                     /translation="MTFEKARDLVDQLTKELNEHNFRYYVLAQPLISDYDFDMKMKQL
                     EQLEKQYPDLAFSDSPTQRVGGAITKEFKQVRHEYPMLSLGNTYSEQEIIDFVTRVKK
                     LIPEKVEYVCELKYDGVAIGLRYENGRLVSGITRGDGEQGDDVTTNIKTIRSIPLRLR
                     ADGYPEKFEIRGEVMMTRDVFDQINEERVEIGEQPFANPRNAAAGSLKMQDSAEVAKR
                     SLDCFLYYLVGDRLPINNHYDSLRECRRWGFKIPNYIAKCSSVEDIFDFIQYWNTARR
                     DLNFDIDGVVIKVNSYEQQQALGFTAKSPRWAIAYKFEAESAATELLSIDYQVGRTGA
                     ITPVANLAPVTLAGTTVKRASLHNADIIEKLDIRVGDVVFVEKGGDIIPKITGVDFNR
                     RPKSSQTTEFIRQCPECGATLIRNEGEAAHYCPNDSGCPPQIKGRLEHFISRKAMNIE
                     SLGEGRIEILYENDLVKSISDFYYLSNESLLGLEKIFPADGEKKERRVSFREKTVENI
                     MTGIENSKQVPFQRVLYALGIRYVGETVARKLAVHFGNIDALIMANTETLTSVPEIGE
                     RIAGSITDYFSKPENIRIIAKLKDAGIQMKTIQAEEPIADGKLVGLSFVVSGVFQNFS
                     REGIKQVISEQGGRVLSAVSANTSFLVAGDKMGPAKIQRAEAIGVKIISEAEFMEMIK
                     "
     gene            42413..43684
                     /locus_tag="PK345_00415"
     CDS             42413..43684
                     /locus_tag="PK345_00415"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HPX33515.1"
                     /translation="MENKRQNRWLVAIMGTLLMIVLGTVYSWSFFQKPIVETYGWSNS
                     GVAWAFSVNILCLGLAAAWGGVYMPKYGPTKLATIGAIMYGVGFMLGALALKMENLAF
                     LVIGFGITGGLGIGLGYVTPVVSASRWFPDKQGFVTGMVVMGFGFGALVMSKIIAPGV
                     MKAVEGNLVSIFFHIGIIILVLGFIAAIFIKQPPADYVPEGYKPPVNKTTDKPVTVDI
                     TPLQGIMTSKFFLMWMLLFINITAGIMFISFQSPLMQDLWEIFKPGADAASLAAIGGT
                     LIAISSLFNGIGRLFWGGLSDKIGRVQTFRLILGTQIIVFIILLFVKNPYLFGMLVCY
                     VLLCYGGGFGTMPSFVRDVFTPKLMSVVYGVILTAWSLGGIAGPQIVAFMKDNYPTNA
                     GHYSVIAGAILLAIGFILSLLVNNNIITDKR"
     gene            43951..45159
                     /locus_tag="PK345_00420"
     CDS             43951..45159
                     /locus_tag="PK345_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33516.1"
                     /translation="MLRKVLAFITFAIILSWQTVIAQDVQKEENKKLQLTPYGFVKGD
                     MFYVTDGVYSFANPNNDYILAPQFASGSGQSAISFTGQHTRLGLKSTVGNKVKVGGLV
                     EIDFFGGPADANTKIRLRQGYASIATGGFEARFGQQWDLFSPNIPNTVNTNGNMWYNG
                     NMGFRRGQIQLSYKWNLEKINPMLQIAFAEGAQESIGLGKDNISGQPMIQARLSGKFD
                     DKYTVGVYYVNAAFKEKYTPAIPPNDHFKFNTTGFGVDFNLPFDKYFSLVGEINSGTN
                     LANANIFSAAGNFFYFGTSDQTRIDFDRKNTGFWLNASSNVSEMFQVILGYGMDKNTS
                     KKFITGNIQSNSTFYADLIFPIKHQFSITLEIGNISTKIVESINNDHKVDKTKTNNAT
                     VIALSGKVAF"
     gene            45360..46241
                     /locus_tag="PK345_00425"
     CDS             45360..46241
                     /locus_tag="PK345_00425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HPX33517.1"
                     /translation="MNEAKKSLNRKIAHQEGWISIIVNSALFVLKYWAGVVSGSVAII
                     ADAWHTLSDTFSSIIVIAGAKLSSKPPDNEHPFGHGRAELIAAVLVAAFLGFVAFEFI
                     QSSIIKLKNREMAVFGALAIIATVTSIILKEGLAQYAFYTWKKTGYLSLKADAWHHRS
                     DAISSVIVLIGIFLGKYYWWIDGVLGILVALMIGYAAYGILKGTISKLLGEAPDKQLI
                     EQIKTIASQETIHDIQLHHIHLHSYGEHQELTFHIRLPNMMTVEESHALVTAIRQKLR
                     DNLSIEATIHIEPYQRQ"
     gene            46566..47603
                     /locus_tag="PK345_00430"
     CDS             46566..47603
                     /locus_tag="PK345_00430"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF008277.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HPX33518.1"
                     /translation="MKRHIYGETEGKQIYQYTFNNEHGMSVSIINYGGIVTHLLVPDK
                     KGNLADIALGYDSLSSYIENNNPHFGGIIGRYANRIAMGKFMLDGNEYQLATNDGLNH
                     IHGGIKGLDKVVWDVKDFIYPDSAGLILTYLSQNGEEGYPGNLNISVTYTLTNNNELK
                     IYYDAFTDKPTPINLTHHSYFNLAGQNSGTIYDHWLQINSKRFVPVNANLIPTGELAA
                     VEGTPMDFQIAKPIGKDIDKVEGGFDHTFVFDNSDNLLKMVAQVNEPKSGRIMRVFTD
                     QPGMQFYSGNFLDGSYIGKGGVAYHKHFGFCLETQHFPDSPNQPGFPSTILRPDEHFR
                     SSTIYWFGTEK"
     gene            complement(47864..49030)
                     /locus_tag="PK345_00435"
     CDS             complement(47864..49030)
                     /locus_tag="PK345_00435"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046835 - carbohydrate phosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HPX33519.1"
                     /translation="MTFDELQLEFKERYQTGEGNLKAVFAPGRINLIGEHTDYNGGYV
                     LPCAISNGTWLLAQSNSGGVLRFLSLNFSETAEIPIDKIGEKSKVSWVNYPLGVINQL
                     LSRGLKLTGLDMLFYGNIPNGAGLSSSASIEVATATLLNHYFNLGINDLEIVKLSQKA
                     EVEFVGVNCGIMDQFAVTINRKNHALFLDCRTLDFELVPLAFDGYKIVICNTGIKRQL
                     AQSKYNERVAECAKAIDFIKQVHPVDYLGQLTLSQYNSLKHLITDSTIRRRVTHVVTE
                     NQRVLDAVNALKNGNIKLFGSLMNASHHSLRYDYEVTGKELDVMVEEALKVDGVLGAR
                     MTGAGFGGCAITILNETAVDSFIDQVGERYKNKTGFIPEFYISETNDGAKMIFL"
     gene            49152..50150
                     /locus_tag="PK345_00440"
     CDS             49152..50150
                     /locus_tag="PK345_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33520.1"
                     /translation="MKVKFYQAFAFPVFTLFLALQLLFNSLNAQQRIPRFTEEEKNWS
                     EYDLTGWQFGINLGMYLANKNSAAFYNGLPGNENNIAYILKNYYWYNEIKQELNGRDI
                     LKFPNTQKPEWTGAGNQWASEYNLKPGDTAWWVYYPLNLKYDAAISPGFYAKYNFNNT
                     TGIFVQSNYVKLKTSGIFQLVIDSITGFSEPALRHGFIRGEEERISIDVGISKHYRTG
                     RATSFFIETGFSLNSTEVLSHKIMIGNREYSIVNVYLNQNWVPNSNVTAYKVYQGGLG
                     YGLFLNGGFKFIFSETISIDPGIQLYFKNINLKPYNEFATNYYFYVRMIFSFFGEI"
     gene            50352..50600
                     /locus_tag="PK345_00445"
     CDS             50352..50600
                     /locus_tag="PK345_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33521.1"
                     /translation="MASPLQLIIKICPLAHRMQSFFPNPAINFIIMDFSLRYKTATLQ
                     LIDLAAASSLSRGSLPTANRRISQAYRLAPTFTVFSTV"
     gene            complement(50711..50905)
                     /locus_tag="PK345_00450"
     CDS             complement(50711..50905)
                     /locus_tag="PK345_00450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33522.1"
                     /translation="MQLPENMPPAPAKIELYSPTGKLLYKAQPLSRYHKIETTHLLSG
                     LYLIRLWDGERCRTEKVVVE"
     gene            51303..52985
                     /locus_tag="PK345_00455"
     CDS             51303..52985
                     /locus_tag="PK345_00455"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33523.1"
                     /translation="MKRIILMLIILIVGTSSFSQSWNWAHSAGGNRPDYAKDMAIDLD
                     NNIYVGITSRSDTCYFKTGYYTLWGETDFFIVKYNSNGNELWVKQCGGPYEPHFGGYS
                     GKDGIIALKYDQTTNSIYGYGGFVGTCNFGEITIGSSSPYDMDIFIAKFNLDGNCIWA
                     KRIGCTNEDERWDRAFSMSIDNYGYLYVCAYLPFSGTFDNTPAEFGGNLAKFDPDGNC
                     LWVKKIIPVQNIGLIPPLFFLYSYIINESLYLTGYNIKSEFTVDTISMSIPNYSGQLI
                     ASFDLNGNIKWLKAIGGPTGTSLLKEIDSDDLENIYIPGAFSGSYATFGIDTIFSDTG
                     RGIYVAKYNKEGTLVWLKHADVTNSANASSVNKDSKGFLYLSGGFSGTINFDSFQLNS
                     ISENDLYIIKFDQEGNFLCGDNTNGGAIYYLRTYPNDIINICGSFFGTANFGNTVLFE
                     KGAVWGDVFVAQHAPINEGINEHNSKISNALIIYANPTTGICNIKIPEELRYEENLSL
                     LVYNNSGKLILQQEINTQEEKISLNLEARAKGIYNAILTNGKQKFQGRVVFE"
     gene            complement(53237..54586)
                     /locus_tag="PK345_00460"
     CDS             complement(53237..54586)
                     /locus_tag="PK345_00460"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HPX33524.1"
                     /translation="MKKNRILNVLMFINLVLIICQLQAQNERSLFFLPDIPQRKLVNP
                     AAFPDHRLYVGVPILSSLKMGFENTLCYNDFFIRKGDSIYFEQDYFVNNLKLNNNINF
                     DLSLEFLSAGIKIKDNYFNFRIADKMNVNTAITKETFQFFLYGNGHSAFLGKDIDLGG
                     NGLNFSYVREYLLGFSKMINPQLSMGMNVKYLNGIANITSNNFSFLLFTDPVDYTIKM
                     KSNVMVNTSLPNKSDFFDIFFNSENRGFAFDVGAEYKYNEKLSATLSAVNVGFVRWKS
                     NLKNYETLDTTEEFVHEGFPLEDYFDSKTLASSALSKALDSIFYEIGIPTNKKEYRTS
                     LPSWVNVNVNYQPKPSHHVGLLYQSRFIKNQNWNTFTLAYTYSLKDKLHLMFSNTFSK
                     STLFSPSAAIGANLGAFQIYLISENFVAPFTFGNAKFYSLQLAINLVFKDEKILDQP"
     gene            complement(54609..>56239)
                     /locus_tag="PK345_00465"
     CDS             complement(54609..>56239)
                     /locus_tag="PK345_00465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33525.1"
                     /translation="FKRSFLLLFTFAILLFSCSKIMDDLDFNKFAQIDWSPEYAIPLV
                     YSEFAIKDFFSEENQKFIKTDDNGLISLVYQSKNLSSDRAEGYFFLDDRHFEHTFPLL
                     IFPSVNPDTITMEFSFTFYSSSESKRIDSVFFKAGTFSIFGKTNLNKEKASMKLTSPD
                     IINVQTNDQLSIDLVLDNPGGQQDWVNFSANESLKDYKLSIDPLLSGGKNRIRVISQL
                     VIQPDSNPDLSPYEIEINGDMTNMEYLDFYGYIGYYDFNLVDTVEISVFNNSLGGAVG
                     FGHDALLFVVETYNEVGVPITIEAQTLQVYSPFTAPYYKDIYLFGQSQPNMFEILSPT
                     PAEIGKSVYTRLDFSNTNFPEVFLSMAPRKLYYDFKANINLSGDTTLQNVLQDTSKLR
                     FQTTLELKLYFEIAKLSIQDTVDFEMSTATLSTIDYLICRVNAYNGFPLNAGLQIYFT
                     DSKYNILDSLIYDNDIRIIEGGKVGLPPKLKVEKPSHKTTDIVLYDSRLERIINAEKL
                     IIKAELSTTNGDLAKIYDDYAFKIKVGAKTGINL"
BASE COUNT        17770 a        11437 c        10384 g        16638 t
ORIGIN      
        1 catcccggtg taaatcctgt aggtgagttc ataattgaca cttcatgtaa cctatataat
       61 tttcaatctc ggcctaatac agaaactaaa ccatcaatgg ttaagaccta tgacaacaac
      121 tttgtaatta atgcaaacat caaagtggga aattatttta aattgtatct ctacaaaata
      181 gattcaaacc ttgagtctgt tcccttagac accacgcaat atgtatatga cagcctttgt
      241 ccgcatacca tccagtcggg cacgatagac cttacggatt gccttgttat acaaagcatt
      301 gatgagcttc cttcgccgga tgaatactac gaaagcatcc ggtggatacc cgtaaaggcc
      361 taccccaacc cggtaagcgg tcaggagctt accattgagt ttgaaaacac ccagcaccac
      421 tccaacattg agctacgcct ttacgatgcc tttggcaaag aggttcatcg ccgccgtatt
      481 tacaccggcc agcaggatat tagtttagat gtaggccact ggccgcccgg cctctacctt
      541 gtcgtagtat atagcaacgg cggcgcagtc gggcagtgta aagtggtagt ggagtaaaca
      601 ggaaaattca taacaatgtc atagtaaggc ttaaagccat gctatgacta aaagctatct
      661 taatcatgtt aagggatagg attttctcaa ttgataagcg taacagcatt gcctcactct
      721 gacaaactta gtgtaagaaa aaaaagtatc acaggggtac acacagagta acgcagagga
      781 aataaaaact ctaaatttga gctttcaaga taacacatca ggaacccgct ctgtaaaatt
      841 gattagattc ttggcaaaat gggattccaa tcccacatta aactcatgta ttgcttaatt
      901 cgggatttat ttctttctaa taaattccaa ttcggtaaag tcaaaatcag gattaggaat
      961 nnnnnnnnnn tattgtcgag gataaaagta cagaagcctg atggcaatga tggaatgttt
     1021 tcaaatgtaa tctcaaaagt atcgaaatgc caatgtttca gtgttccttc gaaaattggc
     1081 gcaggctgca tgtgtactgt aagtttttcg ccatttagct taaccatagc atttccatat
     1141 aattcgccgc catatgttcc ggtataacaa ttcaagggca aagaggtatc ggtattcttt
     1201 attctctctt tatttttttg ttccctgcgc ttttcttcgg cctctttttg ttttttaact
     1261 ctatccaaca atagcatgct ccaatctttc tcttcaccgc caaggaaggt gtcgagggtt
     1321 ttatacatca aaggataata taggtttgaa tttttattgg taaggattac aaaagccaga
     1381 ttttcttcgg gcgccataca tgtttgggaa atcataccat catatccccc attgtgttcg
     1441 actatctttt tcccaagata attccataat ccccatccta atccataact cctaaaggtt
     1501 gttgagggca tatttgtttc gagaaaagag gaaatcggca tttgtgtata agaggtccac
     1561 atctctcgac ttctttccga tgaaaaaata gtatcgccat ctattacacc tctgttcaac
     1621 tgtaaaacaa gccattttgc aacgtcgttt acacaggata tcagcgagcc agccggcccc
     1681 atattatccc agttcaagta ttcaatcggt ataacatcct cgttataatc agtgtgtggt
     1741 gtagcaacgt ttttcttgcc gggcagatca tttgttgaca acaccgtccg gttcatcttc
     1801 aaagattcga gcaactcctc tttaataaac tcctcccagc ttttccccgt aacggcaggt
     1861 attatttcgc cggcagcgat gtacaatata tttgaatagc caaaacgctc tctaaagcca
     1921 aatgcaggtt taaggtggcg tgcacgtttt attatctcct cacgtgagta taaagtccca
     1981 taccaaagca ggtcgccgct aaaagtcgct aaccccgcac ggtgcgttag caaatcactg
     2041 atagtgatat tctcagtaac atatggggcc caaagcttga aataaggaag ataatcggta
     2101 accttatcgc tccatgaaat taacccccga tctaccaaaa tagccaaagc agcggaggta
     2161 aaagctttag tattagaggc tatcgggaaa agtgtttcac tgtcaacggg ttctttcgtc
     2221 cttacatcct ttacgccaaa gcctttggca taaatgattt caccatcttt cactattgca
     2281 atggccatac ccggaacctt ccagtcggaa agcgctttag cataatactc atcaagaatc
     2341 gaaagtttcg actgatactc cgactgagca aatagagaaa aagaaaagtt taaaccgatt
     2401 agggcaaaaa tcactaagga ataggtttgt agttttcgct tcataaaaat ttcctccttg
     2461 tagttgaaag gttattaaat tattaaagac aaaaataggt gaaattatta gctaaatata
     2521 ttaacgctgt ttaaagagat ttaatcccgt ttatttattt ttggggatgt ccgatatggg
     2581 tttaagaatt tgtttaccag cttcctccgg cgccgccgcc gccaaagctg ccacctccaa
     2641 atccgccaaa gcctccgcct cccccgccac taccgccaaa cccaccaaac aatcctcctc
     2701 ctgaattgaa agagccccac ttacccttct ctctcccttt gtccgcctca aggagaaaca
     2761 tcaacaattg cattgcagag ataccgtgat ggccgctgct ataattttta acctttacat
     2821 tggccatttt gagctttcga taaacaaaag caatgaataa gactaatatg agaaaaacaa
     2881 aaaacaagcc ttttaacaca tccctatttc caagatattc atctgccgta aactctccgg
     2941 caacaagctt catgatgata tctgtagtgt ggtcgagccc ttgataatat ctatcagttt
     3001 taaaaaaagg gatcatctcc tcttccacca atcttttaag tacagcatcc ggcagcgctc
     3061 cttcaaggcc atatcccgtt gcaataaatg tttctccttt ttctgtctcg gtttttggct
     3121 ttaccaataa aacaacgccg ttgttcttgc ctttgtagcc gacaccccat ttttctccaa
     3181 tcaggtcggc atactgctct ttttgatatc cggcaaaatc atttacaata acaatggcaa
     3241 tttgattgga agtttcgtta ttgaaagtta caagcttttg ctccaaggtg tgggtttcag
     3301 cggaacttaa aataccggta aaatcgttaa ccaattttgg tggagaagga cggtcgggga
     3361 tatccttttg tgcaatggca aagtggcaat tgctcaataa caaaacaacc aacagcacaa
     3421 aatacttttt cattatttgt agctattaat ctgtttttgg aaatttctca taagaaatat
     3481 cgtcaggcaa ttcgttgata tcatcatcag gataaggaaa atatcgtttc aggcgttcgc
     3541 ccgtcagctt gataccatgc actaatccat cggcaaattg gccatttgca aaatatactg
     3601 caagtttatc tctaacttct tcccaaaagt tatcttctac aacggcatta ataccggcat
     3661 cgccaatcac tgcgaactta tggtcattca tcgaaagata aaagagcgct ccgttacgaa
     3721 actttgtttt atgcattttc agtttttcaa aaacatatgc agctctatca agtacatcaa
     3781 cacggcagtt ctcttcgata tgcacgcgta tttcgcccga ggttttaagc tcggcatcaa
     3841 taatggcctg cctgacagac ttttcgtctt cactggaaag tcccacatta ccctttttag
     3901 aatcggcttc caatcgaaaa agatgatttg ttaaaattga acttccggaa ctttttcggc
     3961 gccctcagca gcttcaaaat atggttttgt ccgaaatcca aagattgacg catatatatt
     4021 tttggggaat tgcctgatat aaaaattgta ctcttgcgtt gcctcattaa attttcgacg
     4081 ggcattggcg attctgtttt ccgtaccttc caactgtgct tgcagatctc ggaaattggt
     4141 gttggctttg aggtcgggat aacgctccac agtaacgagc agcctgctca acgccgagct
     4201 aagcgccgcc tgcgtttgtt cgatcatgcg caacgagttt tcatcaaggt tatcaacatt
     4261 gaggttaacc tttgaggcat tggaacgagc ctcgataacg ccaataagag tctcctgctc
     4321 aaagtcggca tagcctttca ctgtgttgac aaggttagga ataaggtcca tccgccgttg
     4381 atagtcggta accacctgcg accacgcaac attaacgccc tcatcatatt taaccattga
     4441 gttgtaggtg ttttttgcac ctgaaaagat caaaatcact actaaagcaa ttactccgag
     4501 agtaacatat aatcttctgt ttttcatacg aaaagatttg aaatatttgt ttaagcaaaa
     4561 gtattaataa attcaaattt agcgtaaatc aacccactgt acaaactaca atatctcaac
     4621 gctgcgttta ataaactttg taagctcctc ccctttcaga aggttttgag aaagcagcgc
     4681 cagatctgtc agttgctgtg ccattttttc gcggctttcg tcattttcct catttgccat
     4741 cttatacacc aggttatgat tactatttac cactaagtta tacctatcgg ggaaagcgtc
     4801 taagccgcta tagccgccgc caagcgccga catatccttc atcctgcgca taaactcggg
     4861 ctgcgttatc atcaccggca tatctgtctc actcaggtta tcaaaaacta tcgtaaactt
     4921 cgacttgtca acagcctttt caaaaatagg tttaagcaac tccttctgct tatcgtcaag
     4981 tttcgaaata ttttcatcat ccgaaatttt tatcagctta tcaatagtat ctgcatcaac
     5041 acgtacaaaa gatgaatttt cgaacttgtg ctcaagtgta tttacaaaat gattatccaa
     5101 cacaccatcc atcatcagca catcgtatcc gcgttcagta gccgattcga tatagctatg
     5161 ctgtgtttct ttgtcggtag cgtacagata gactaacttt ttatccttat ctgtctgggt
     5221 ccctttgatt ttctccttgt aatcgtcaaa ggtgaagtac ttatcctcta tattttttag
     5281 taaacagaac tttttagcac ggtcgtaaaa cttctcctcc gaaatcatcc catactcaat
     5341 aaacaccttt atatcatccc atttcttctc aaattcctca cgttggtttt taaaaatatc
     5401 ctctaatttg tcggatactt ttttcattat atggtttgag atttgcttta ctctacggtc
     5461 gctttgcaga tagcttctcg acacgttgag cggtatgtcg ggcgagtcga ggacgccgtg
     5521 tagcaatgta aggaaatcgg ggacgatgcc ctctaccgaa tcagtaacaa aaacctggtt
     5581 cacatatagt tgtatcttat cacgctgcac ttcaaaattt cgcttaacct taggaaaata
     5641 gagtatcccg gtgaggttaa aaggataatc aacattgagg tggatatgaa aaagcggctc
     5701 ctcgaagttc atcgggtaaa gctcacggta aaaatctttg taatcctcat ctttcaagtc
     5761 aaccggggct tttttccaca atgggtcggt attgttgata atattaggct tctctacctc
     5821 cacatacttg tcgttgccat ctttatcttt ttcgccttct acctttacat gctctttttt
     5881 agttccgaac tgtatcggca ccggcaaaaa gctgcaatat ttttttagca actcacgtat
     5941 cttgtaatct tccaaaaact ctttttcctc ttttgaaata aaaagggtta cttccgttcc
     6001 acggtctgct ttatcgtcct cctctaatgt ataatcgggg cttccatcgc aagtccattt
     6061 tactgctttg gtcgggcctc cctttttata tgtcttggat ttcagttcaa ccttgtcgga
     6121 taccataaat gaggagtaga agccaagccc gaaatggcct attattgcat tttgttcggc
     6181 ttcagttttg gttttaaact tcttcacaaa ctcctcagcg ctggaaaagg caatctggtt
     6241 tatatactta tccacctcct cggcagtcat cccgataccg cggtctttaa cagtcagtgt
     6301 tcctcttttt ttatctatgc ttacgtgaat ggtcaaatcg ccaagctctc ccttaaaatg
     6361 gcccgtactg gcaagggttt tgagcttttg agtagcatct acggcgttgg agatcaattc
     6421 gcgaagaaaa atttcatggt cggaatagag aaatttttta ataataggga aaatgttttc
     6481 agtctgaact ttgattttac ctttttgcat atctaattat tttataatga ccgcccggaa
     6541 atcaaagcct atgccatgca aaaagatgct gacattttga caaatagaga taatgtaaac
     6601 aattataggt aatcggatca aaaacagacc taagcatcta agccttccga cttcaatcct
     6661 ttgccaagca aaaaatcaaa cacttcatat atcaatttca catttccgca aaggtgatag
     6721 cttccttatc aacagggaaa cagccttaca aaactgcgaa acggcgctat acctacgccg
     6781 atagctgtaa ggcatcgctt aggaatggat ttgatctcaa caactgttta cctaaagcaa
     6841 caaaagggcg caaacagata aaaaatatca ggtaatgttg ttttctagac aacaccgttt
     6901 tggggaacaa aaaaaaccct gactagcagc ggttttcgat agtgtcaggg taattttttg
     6961 aggtaaaaaa atcaatagcg ctgactttta aactcctcca tctctttttc aaaagtctct
     7021 ttaaacttct cgtactggaa atcgagtttt agtttttcgt catccgaaat cctttttatc
     7081 actccattag ctatctcatc tccggccagc tcgatagcca cataacattt ataatttcca
     7141 tcggtagttt ttgtgtactc ttcgcaaata atcctgacac catttagctt ttgattcacc
     7201 acctctcgcg aaagaccttc gtatctctcc ttgtcttcga ttgttttgtc aacggcatag
     7261 ctgttgaaat aattatcaat cacagccttt acggtaattt ctattgagct tgcgagcgcc
     7321 aggcgtgcat tactcatcgc cttgcgcttt gccattgatt ggtttgcact ctcggccaca
     7381 tcgtttgccc gaaaatattt gttatcggta aaatattcag ggccagagca gtaaacaata
     7441 atcttttctt cgcctgaggc aactttaaca gcctttttgg acgatttgca actatgaaat
     7501 agaggggttg caacaaaagc taaaagtagt aaagcgccaa acgacagtaa cggatacctt
     7561 ttcataaata tttatttttt attaatgatg atgcaaatgt aattcatttt cttttcaaca
     7621 gtttgattta aataaaaagg cttaatcatc ggtgtttaat ctaagttatt tactaaagca
     7681 cggatgcaaa tttttatcca ggtattcgtt accacatagc atctcttaca aacaaaaaag
     7741 gctacttttc taagcagcct ttttgttttt atcattttaa aaaattttta cgctcctaag
     7801 ccaaagcgat agaacttgac tactgtcagg tttttgtcgg attctttcag gtagttgcca
     7861 acggtttttt tgctatcttt aataaattcc tgattgagca atgtgctctc tttgaagaac
     7921 ttttgcagtc ttccttgagc aattttctcg agcatatttt ccggtttccc ttcctggcgt
     7981 gccagttcca ttcctatttc aatttcttta tcaatcatct cttttgttac atcgccttta
     8041 tcaattgcta ccggattcat tgctgccacc tgcattgcca tgtcatggcc ttttacggca
     8101 acgtcggcag tcttcagatt aagccccact aaggttccta ttttattgcc taaatggttg
     8161 tacgatgata caaaaggagc ttctataaac tcatagctat cgaactccat cttttcgccg
     8221 gtcttcccaa ttaaatcgct aagagcctgt tctacggtac ggtcttcaat ttttaatcca
     8281 cgaactgctt cgagcgatgt agctttattc ttgagcgcta acgaagctac tgttttcaca
     8341 aagtttacga actcttcgct cttacctaca aagtcggttt cgcagtttat cataactaac
     8401 acgccaaagc tgttttcctc gttcgtctga gccataatat acccttcggt agcatcttta
     8461 tcagctcttt tagcggctaa ttttgctcct tttttgcgta atatctcgat agctttttca
     8521 aaatcgccat tgctctcggt caaagcctgt ttgcaatcca tcattccagc tccggtttgt
     8581 ttacgaagct ttgcgacatc tgctgcttta atatttgaca ttctcgttct attttattta
     8641 tttattaatt aattatttct ttcttattgc cgttcccttt ctagctcttt tctctttttg
     8701 ccctgtctct tcctcttctg tcttcttcag ctttcgtatc tcctcttttt ttattgttcg
     8761 ctcatcctcg tctttatcct cttccagttc tgcctcaagc tcctgacggg ttctggtttt
     8821 gatgctttct ataccttcat cttcctcttt aaactgttta tctttttcca ctttcctctc
     8881 catcattccc tcctcgatag ctctaaccat tatctccact atcagggcaa ttgattttga
     8941 agcatcatca tttgccggta taggaaaatc caccaattta gggtcgctgt ttgtatctac
     9001 catagcgaat gttggtatat ttagctttcg ggattcggca actgcaatat gctctttcat
     9061 gatgtccacg ataaacaatg ctgagggcag cctatttagg tcagcgatac tccccaactg
     9121 tttttcgagc tttgctctct cgcgggtaat ctgcagacgt tctcgttttg agatattggt
     9181 aaacgtggga tctttcatca tggtatcaat agacgacatt ttgcgtaccg ctttgcgtat
     9241 tgtagcaaag ttggtaagca taccacccgg ccagcgttcg gtaacataag gcattccaac
     9301 acttttcacc ttttcggcta caatatcttt tgcctgtttc tttgttgcca caaaaagaat
     9361 tttcttaccc gactttgcga tctgttttaa agccgatgca gcctcctcga tcttagctgc
     9421 tgttttgtaa aggtcaatga tgtgaattcc attgcgctcc ataaatatat agggcgccat
     9481 attagggttc cattttcttt taagatgccc aaaatgcacc cctgcattga gcaattcttc
     9541 aaaatttgtt cttgccatta attttttatt ttatgtttac attccgatta agcaacccgc
     9601 aagtagttca aagcgcgatg aagtctcacg ggtttggata ctaaacacct taatatccgg
     9661 aaattatcgt ttactgaact ggaatttttt acgggctttt ttctggcctg gtttctttct
     9721 ctcaaccatt cttgggtctc gcataaggaa tccattggat ttcaggattg ccctgtgttc
     9781 ggggtcaatt tttataatag cccttgaaat tgcaagacgc aaagcctcag cttgtcctgt
     9841 tataccacct ccgtcaatgt taacgtgtat gtcgaactta tcaacattat ctgtaagttc
     9901 taaaggttta cgtacaattt gttgtaagga ctctaccggg aagtaatttt cgaaatcacg
     9961 tttattgata gtgatctttc catttcctgt ttgaacgaaa gctcgtgcaa cagcggtctt
    10021 tctcctgcct actgcattta ttatttccat tattatttaa tttcgtttaa attgattttc
    10081 ttaggttttt gtgcctcctg tttatgctcg gggccggcat acacatacag gttgcggaaa
    10141 agctgacttg cgagcctatt tttggggagc atcccacgta tggcattctc aacaataaat
    10201 gtcggtttgc gcaatttcat ctgtttggct gttagtactt tctggccacc cggatatcct
    10261 gaatgggtaa tatactcttt atccgcccat ttgcttccgg taagtctgac tttatcagca
    10321 ttgataatca ccacattatc tccacaatca acgtgtggag tataattcgg cttaagttta
    10381 cccctgagca gtaaagctgc tttagaggct aaccttccca acacttcgtt ttcagcatca
    10441 atgagaaccc agtcctttgt aaccgtctct ttattagcgc tgatagtttt gtaacttatg
    10501 gtatccattt attaatcagt taaatctgtt catcgaaaat ctcgtttcta aaacgggctg
    10561 caaaagtaga tgttttttta ttgctaacaa gaatcgtttg aaaaaaaatc aaaaaaaata
    10621 tgcttgcaga tcatttattc ataaataatt tcccaaataa ggcaattgga ttagagaata
    10681 tgttaaataa caggccacgg aagcgtttgt ttttttactc taaatgaaat aacgaaaaaa
    10741 ttactgttta accaaaaagt tgcatttgca gcttaactct acccgattaa aactataact
    10801 ttaaagcagc tatttgatct tcaagaattt ttattttatt ttccgcatcc gcctgttttt
    10861 gtctctcttt ctctactacg gcaagcggcg ccccgctaac gaacctatcg ttgccgagct
    10921 ttttcaacac cgcagcaagg aatccttttg tgtagttcag ttcgtcttca agtttttgaa
    10981 tttcttgttc aacattcact acatcgccaa gaggaaggta aaactcagtg gtttttacaa
    11041 caaacgacat ggctttatcc accttttcat ttacatattc aatcttcgta aggttacata
    11101 gtttagcgac aattccatca aatgtgctgt cgggtttttg atcgaggttc ttttttatat
    11161 agaggtcaag tgtttgtctc atcgctatgt ttttctcagc ccttacagcc ctcaaagcaa
    11221 taatgacctc ttttgcaaac tcaaactgat taagtatcaa atgatttatt ctaccttttt
    11281 ttggccattc ggaaatcatg atacaatcgt cttgtgttcg ttcacacaag aggtgccata
    11341 tctcttcggt aagaaatggc ataaaaggat gcaaaacttt catcagtccc tcaaacagtg
    11401 ttatggtatc gtcgtatgtt tttcgatcaa caggattatc ggagccaggt tttatcattt
    11461 cgagaaacca tgaacagaaa tcgtcccata tcaatttgta agtaaccatc agagcatctg
    11521 atattcggta ttttgcaaag tggtcgtcaa taagtcctag tgttttgtta aatgttgcgt
    11581 tgaaccactc tattgccact tggttagtgt cgggctgagg caggttgtca tctactttcc
    11641 atccttttaa aagccttaaa gcgttccaga ttttatttgc aaagttgcgg ccttgctcgc
    11701 ataggttttc gtcaaaaggc aggtcattgc cggctggtga tgttaacaac atacctactc
    11761 gtgtgccgtc ggcgccgtaa tggttcatca acttgatagg atcgggtgaa ttaccaagtg
    11821 atttcgacat ttttctcccc aacttatcgc gaacgatacc ggtgagatat acatttttaa
    11881 aggggatctc tccccgatac tccaatcccg ccattatcat tcgtgctacc cagaaaaaga
    11941 gtatctccgg cgctgtgatc agatcattag taggataata atattttata tcggggttat
    12001 ctgggttacg aattccatcg aaaacggaaa ttggccaaag ccatgacgag aaccaagtgt
    12061 cgaggacatc ttcgtcttgt ttcaactcct cttgtgtaag atcagcgagt tccggaaact
    12121 gtgattttgc aattgtgata gcctcttcct tcgatttggc aaccaccatt tcgttgtttg
    12181 gcaaataata cacaggtatt cgctgccccc accataattg tctggagata caccagtcgc
    12241 gtacattctc catccaatgg cgatatatat ttttaaactt cttgggatga aattcgataa
    12301 catcatccat taccacttta agagcgggtt cagctaactc cggcatttta agaaaccact
    12361 gaagcgagag ttttggttcg atagctatgt tagtgcgttc cgaatagcca actttgtttt
    12421 tataatcctc tattttaact aaatgcccgg catttttaag atcgtcaacg atctttttcc
    12481 ttgccaccat ccggtcttct ccaacgtaga gctgggcggc agggcttaat gtgccgttat
    12541 cgtttaagat atcaattgtt tccaacttat gccttaatcc aatttcgtag tcggccaagt
    12601 cgtgtgcagg cgtaatttta aggcatccgg taccaaattc catatctaca taatcatcct
    12661 ggataattgg tactaatcgg tttatcaatg gcacaaatac ttttttaccc ttcagatttt
    12721 taaaacgttt gtcttttggg tttacgcaaa cagcggtatc gccaagtatt gtctcggggc
    12781 gggtagtagc aattgtaacc cattcatcgt cagtgttgtc aatttcatat ctgacgtaat
    12841 aaagctttga atcctcttcc cgataaatca cctcttcatc ggaaagggcg gtaagcgcct
    12901 gcgggtccca gtttaccatt cgtacaccac gataaataag ccctttatta tagagatcaa
    12961 taaacacatc aatcaccgac tcataaagat cctcatccat tgtaaacttt gtcctctccc
    13021 agtcgcatga agcccctaat ttttttaact gttcaaggat aatacctcca tgtttttctt
    13081 tccactccca ggcatgactc aaaaactctt cgcgcgtaag gtctttcttg tcaatgccct
    13141 cttttttcag cttttcaaca acctttgctt cagtggctat ggaggcgtgg tcggtacctg
    13201 gtaaccatag ggcgtttttc ccaagcatcc tggcacgacg caccaacaca tcctgcaggg
    13261 tgttatttaa tatatgaccc atatgcaaaa cgcctgtaac gttgggagga ggaattacga
    13321 tacagtaagg ttcacgatca tcgggcaccg acctgaaata gcctttatca agccagtagc
    13381 gataccattt gtcttcaacc agcgtaggat catatttaga tggaatcatc atagttggat
    13441 aagttttccg aataaaaatg gctgcaaaaa tatgaattgt ttttgggaag aagtaaattg
    13501 agaaatgagt aagtgaaatc cacattaaaa taataaacct tatcgggttt tcgctattct
    13561 cttcttaaaa acgtcaattc caccatacgt aagtcgttga atcgtgttac aacataatcg
    13621 gctctactgc ttctcacact aaaatcaata gaacaggtaa ggtgaaagtc ctgatcaatt
    13681 tgatctaact cttcctcttt gaggatacgc atcacatgat tcatgtgttc gtatgcaaat
    13741 ttgacctgat atatatcatg gatgatacgc gtttggatga cattgttagc aagggcatcc
    13801 tgagcagcaa atttatatgc attgataagg ccgcgcacgc ctaacttaat cccgccgaaa
    13861 taacgtatta ccactatcac ggtatttgtc agatcattgg atagcaattg accataaatt
    13921 ggcttcccgg ctgttcctga cggctcgcca tcgtcgttat aacgataagt ttccttgttg
    13981 tatcctaaaa tccatgcata acattgatga cgggcatcgt gatactgatt tcgcaacagc
    14041 tccaactctg tcctgatctc ctcctcactt tttactggaa tagcaaaaga gataaaccta
    14101 cttcccttat ctttatagag cccttgcgac ctttttgtta ccgttttata ggtatcatcc
    14161 gaaagcataa ttgatttatt ttcgacaaaa gtagaaaaat cacgtcaaca tttagtagcc
    14221 gccgatcacg ggttaaacac tattttaaaa cgtatcgttg aattaaaagc cacaaaagca
    14281 ttgataaaac tctatgaaaa tgactcggcg ccaatgccct ggcgctttta ttataatcct
    14341 gttacctgtg aaatgtaggt atttcagttg gaaaaacaag caggttacta tcaggaggac
    14401 gattctggaa atataatgcg tatccgtacc tccgactcgc ttattatcaa tttcgtcttt
    14461 aaccgtcaga tgcggcgtta ggctttattc gcactttcaa aatatgaaga ggcgcctacg
    14521 gcatttattg ataatattcc aacacttgcc gaaggattca tacttattga aaaaaaaaga
    14581 tcaaaactat cgttaacagc ctcaataacg gaatccgaga aacagctttt ggaagtcgaa
    14641 ctcagcacag cagtaagtat tgccgaacac caatcattgg tttttggcaa aaaagatctg
    14701 agaattaaga tctttccaat aatctttcat ttaacctctg attatccttt tgaaggtatg
    14761 ccggaagtgt cgtttgtgga ggattttacc aggatggcca aaatgaagat cacaaatttc
    14821 catggtcaag aagtcgcaaa tgttacttta gccacaattc ctggccgcaa caggataaat
    14881 ctttatgccc atttcgttga tggatcaaaa gaagaccttg agcaaattat gagttttgtg
    14941 gctacagtaa cattggaaag caagctatcc ggattttagt gacgatagta acttgttact
    15001 atagcagcat ggccaaaatt attcccattt ggaacgattg acgtacctgg caaaaaaatt
    15061 caaatttggc ctgacaagta tgtcgcccgg cctaagttca tcaatcccat ctacacaata
    15121 gtaatttaag acaaaagaat tatcattttc tttagtcgaa aagatcccgc acaagataaa
    15181 aagcaaggca actattggaa aacaaaactt tataattatt tgcaaattgg aaaacattag
    15241 tcgaaagaat taataattat taaaattact taaaaaaaga taaaataacc tgtaatcaaa
    15301 aacgtttatg ctacttttgc acctatttaa tttaactatt aacaattttt tttacacaat
    15361 taatcaaaac gacatgaaat caagaattat ttttagtgct atgctaatag cttttgcttc
    15421 gataacaatg ttttcctgca aaaaagacaa aaaagactct ccaaaagagc tgactcctga
    15481 agatgcgaaa attgaactca atgatgcttc gcagcagttt gacaatcaaa tagtcgagat
    15541 tatgcagact gaatctgttc aaacggcaaa tttcctattg gaactactgg gaattgattt
    15601 agagtttgat ttatacaaaa atttattaga gcaacctggg aatattcatt tgacaaaaat
    15661 taaaaatgct ttaaaagccg ctaattcaac aaactcttct aacgagcttg aagagtatgg
    15721 gatctatgaa tttgattttg attctgaaga attcataatg gttgaaccta cagatgcttc
    15781 gataattttt tattttcccg ctgacgaaac agcatatatg aatatgcaaa acaatgccga
    15841 aatcgcaatg acaaacatac agactacaat agtcctttat acggaggaat attgggattg
    15901 ggagcttgag caatgggtaa cagagactta tgaagaggag gtgccaacta attgtaaaat
    15961 aacagaaaaa gtggatggag caactttggc aacaataaat tataactcaa caataactga
    16021 aaatggattc ccaacagcct tcaatgttag tgcagatgtt cctccttaca atataaatac
    16081 aaccttaaac ggtagcggta caaaattcca aacacaatta tcatttaaga aaaatagcac
    16141 ctcattgttt agtttcgatc ttactattga atatacctcg gatatggaaa atattaaaaa
    16201 gattaatggc aacctgactc caaatccttt aaaaattgaa ggttttatca atatctttgc
    16261 cattgaaacg catatgtatg aacacgaatt tagcggagat tgggatattg attatcttaa
    16321 tgggcaaata gacttagagc ttattcaggt agaaaaaaat gctaagatag gcgatataga
    16381 atacaaatct tattatgatt atccagcttt agcagttgtt tattcggatg gtacatttga
    16441 ttggctggag aatgttatta acataggttt taaacataga agaaccagaa aataataccg
    16501 gtttcaggta taatgatagt tcaatattgg attagaatta tacttcatta atcgtacctt
    16561 atttgaaaac aggctacaag agcatcactt gtagcctgtt tccgtttaaa tagttttcat
    16621 ttgagtatta gttattggcg atctatctaa aaaaatgtct tcacgataat ttaaaactta
    16681 ccacatacat ttgatgggtt tacacatttt atttttcttt gttccataaa aacaatttat
    16741 caagttcggt ataagaaatc ataaacgcta caatattttt caatatttat ttaaaaatga
    16801 aactactatc atctctccta tcaacagcat ttgttttatt aatgttatta tcttgcaaac
    16861 atggcgcaac cccttttcag atgagcgatc cggcagacta cgataaaata attgtcgggc
    16921 atactgccgg tattatatct tcacaaagcg cagtacaggt tacttttgcc aaagcaatag
    16981 aaagctacca acccggcgcc gaattgccag cagacgtatt cacctttgtt ccatcaatat
    17041 caggaactgc tacagtcgaa gattcgtatt ccatttcatt taagccgaac aaacctctga
    17101 aacaaggcat caaatatgct gtgagcctca accttgttag gctgatggaa gtacctaagg
    17161 aactaaaaaa attcaatttc ggatttgaag taatcaccca ggatttcact gtattagaag
    17221 ggcatctgat gcctgaatcc attgatattg ccgacaagta cctttacaaa ggaattataa
    17281 ttactgctga cctaatgagt gtggaagagg tagaaaagct tcttaaagcg tccgctcaaa
    17341 acaaagacct taatattcaa attgaatccg aagggataaa aaagttctcg tacactatta
    17401 gagatatcga gcggttagac atagggtcta aagtaacctt gtcatggaat ggagataaga
    17461 ttggattaaa aaagagaggg gatttggata ttgtaatccc tggcaaaaac gagtttatcc
    17521 tgttaagcca tgaggtaaac catgagaaca agtcgttgca gttaattttt tccgatccta
    17581 ttgacaataa acaagagatt gcaggattaa tcggtttcca aagtaccgat gatgtacgca
    17641 tttcacgcta tggcgctata gttaccctat ttcccaatca aactttgatt gacgaacaag
    17701 tagtaaaaat atctgaatgg ctcagaagcg ccggaggaat aacccttgga aagcccatca
    17761 ctttatttgt gcaaatggat gcattgaaac ctcaggtgga gatcatcggg aatggaaata
    17821 ttatccccga ctctaaaaat cttttcctgc attttaaagc ggtaggatta aaagcggtag
    17881 atgtagctgt ttataagatt ttcgcaaaca acataaagca atattttcag gaatttaccc
    17941 ttgaaaattc cggcgaatta cgttatgttg gtaggccggt attcattgaa acagtaagac
    18001 ttgacaagga tgataaaacc gacctaaaca gatggaatgc cttttccgtt gatctgaccg
    18061 agatggttcg aaaagatacg gaagctattt accatgttaa aatttcattt aagagaaact
    18121 attcgctgtt taactgtaac aatgaattaa cagatgacaa tgagattgaa caagcggtaa
    18181 ttaccgaaga agaagtggat tattttgatg ggaagtccta ttggtactat tactggcccg
    18241 aaaattacaa ctggtatgag caggacgacc catgctctga ttcttactat attaacgacc
    18301 ggtttcccac ccgcaatatc ttggccagca acattggcat tttaaccaaa tcagttgata
    18361 acctgaaatt ttcggtagca ataaccgatc tactcaccac caaaccaata gatggctctt
    18421 tggtagaatt tttcaacttt caacagcaaa aaatcggcga ggtatccacc tctgcttccg
    18481 gcatggccga gatagagctg acaagtagtc cttaccttat ctctgccagt tatcagaatc
    18541 aaaaatcatg gttaaaagta gataacgggt cttcactctc gttaagcaat tttgatgttt
    18601 cgggcaaaga gattcaaaaa gggataaagg gatttatcta tgaagaaaga ggcgtttgga
    18661 ggcctggcga cacactcttt attacctttc tgatggatga ccgcttgcaa aagctcccga
    18721 ataatcatcc cgtactattc gagcttttcg actctcgtgg caatctctat aaaaagcaga
    18781 ttaaaaccga aggagttgac aatttctaca cctttattac accaacaaaa cccgatgcac
    18841 caacaggaaa gtggctgata aaaattagtg ttggcggggc catcttcgaa aagaggatca
    18901 acattgagaa tgtaaagcct aacagattaa aaatggacat ccgttttgat gataaagtgc
    18961 taaccactaa tagcctacac aaaccagggt ttctgaaatc gagctggtta cacggaacgg
    19021 ctgcttctaa tttaaaagct acggtaaacc tgaaactttg tccaatacaa tacacattta
    19081 aaggtcatga taaatatcaa ttcaccgatc cgacaaagac attcaatccg attgaaaaca
    19141 taatttttga tgcacctctt gactcaaatg gcgaggcgcc aataagcctt caatctatga
    19201 gtaacatcca ctccccagga atgttaaatg cagtgtttac taccagagtt tttgaatatg
    19261 gcggcgattt cagtacggat gtttttgaaa ttccattttc tccgtttgat gtctatacgg
    19321 gcattaacat cacaggcgca gatggaatag aggatgtgct tgaaactgat aaggatatag
    19381 ctgtagaggt ggttacaatt gatcacaagg gtaatgctgt ttcgcaagat aacctcgaac
    19441 taaatattta caaattatca tggcggtggt ggtggagttc taccaacgac aacattgcga
    19501 gttgggtaaa cggacaaggc actgagctga ttgaacataa aacgttttat acaacgcaag
    19561 gaaaagcacg ccaacatttc aaaattgaat accccgaatg ggggcaatac tttgtacagg
    19621 tagtaaatac aaacaatcgg cattcaacag gaaaaatcgt ctttgttgat tggccaaact
    19681 cgttcaaccg ttcgggaaga aaacatccgg cagcagctac aatgctctct tttacgactg
    19741 acaaagaaaa atatacgaca ggtgagaacg ctactgttac ctttccttca accgaaggcg
    19801 ccaatgcaat gataagtctt gaaacaagta gcggaataat aaaacagtgg tggcaggagt
    19861 gtgaaaaaga taagacaagt ttcgatatca ggatcacccc tgaaatgact cctaacttct
    19921 ttgttagcat tacccttatc caacctcatg gcagcacagt caatgattta ccaatacgtt
    19981 tatatggcgt gattccggta atggtcgaag aacctcaaac acgccttaca cctgaaatac
    20041 gcgtggaaaa agagatccga cctgaggcac cttttacaat taaagtatcg gaaaaaagcg
    20101 ggaaggccat gacatatacc cttgccattg ttgatgaagg gttattgggg cttacgcgtt
    20161 ttaaaacccc cgacccgtgg agcagcttct ttgcccgcga agcacttgga gtaaaaacat
    20221 gggatatgtt cgaccaagtg atgggcgctt acggtggaga attacaaaaa gtactcgcta
    20281 tcggtggtga tgatgaactt gccagagccg gtgaaaaaaa ggcaaaccgc tttaaacctg
    20341 tagtaatatt ctccggccca ttcaccttga aagctggaaa acaaggttta caccagttta
    20401 cgatacctaa ttacaccggt gaagtacgga ttatggtaat tgccggtaaa aatggctcat
    20461 ggggagcaag cgagaaaaat gttaacgtta aacaacctat aatgctttta cccacactgc
    20521 caaggaaatt aagccctgat gaggaggtta taatgccggt cagtgttttc tcgatgagta
    20581 acaaagttaa aaaagtagcg gtcaaaatta agaccgacgg gcatctgatc ccgttaggta
    20641 atacttatca agaagcagat ttcagtgata ttggcgagaa aatggtttat ttcagcctaa
    20701 aagctgatgg gtcagaaggg tatagctctg taagccttga ggcaacatct ggatcggaaa
    20761 aagctacagc ctcggttgag attgaggtaa agcatccaaa tccatatacc acaatctccg
    20821 aaagccatat catacaggag ggagctcaac agaaaataga tatcacacca catggtttgc
    20881 cgcaaaccaa ttcagtaaaa ttgaacattt ccagccttcc gtcatttgaa attgaaaaga
    20941 gccttcaatt cctgatcaac tatccttatg gctgtttgga acagattgtt tcctcaagtt
    21001 ttgcacaact atatctttcg gatcttatcg aactcagcga taaccaaaag gtaacaaccg
    21061 accgaaacat cagagcgact ttaaaggctt taacaggtta caacaaaggc ggcggccggc
    21121 tttcatactg gccgggcagc gccacctctt ccacatggag cgaagtgtat gcgggtcatt
    21181 tcatgtttct tgccgaacaa aagggctact cgttgcctgc caatctgaaa agttcctggc
    21241 ttgaagcaca atcacgctta gccatgcagt ataatagtgc ggcggaacaa gagaataaac
    21301 acgatattaa cacccaagca taccggcttt atgtactggc attagccgga aaaccggtct
    21361 tcagcgccat gaacagatta aatgaaacta acaatattac aaaggaggca gcatggaggc
    21421 ttgctgcagc atattatctg gcaggaagac cggaaacagc cgagaagttg ataaaacaaa
    21481 ctgataagtt gcaaaccacc gaacactatt ttatggatga aaattttggt tcatcgttac
    21541 gcgatcaagc aatgatagtg gaaacgatga cgctgatgaa taaaaagcaa gaggccttta
    21601 accaacttaa tcgaatggca cggcaataca atgccgaaaa tgcttcaacg caaaccgcag
    21661 cattttgcct ttatgctctt tcaagatatg ctaagctaac cggttcagag gccaatctga
    21721 atttcgattt aaccgtaaat aacaaaaagg aaagtctgaa ctccacttcg ccggtttatg
    21781 aaaaggactt gggaaaattt gacaatccaa gtagtataac catccaaaac aaaggaaaag
    21841 gagatttaat aacaacaatt tcggttaccg gccagtcgcc atacagtaca gaacaaacaa
    21901 cggcttccaa cctacgaatg cagacacgct ttttatcaac tgacgggcgc ttaattaatg
    21961 ttggtagtat aaaacaagga gatgatttca ctgcagaaat aagtattaca cacccaggta
    22021 cgggaggcga ctataaaaat atggcgctta attttgccgt accttccgga tgggaaattt
    22081 tgaatagacg gtttcaaaat acggaagttg tagtaatgga gagcaaatat acttttagag
    22141 atattcggga cgacagagta gatacctttt tcgacctgcc tgccggaaaa acagttatct
    22201 acaggatcga gctaaatgcc tcatacgaag gaaaatatta tatgcctgcc gttaaatgtg
    22261 aagcaatgta cgaacgctca atttatgcta tagaaaaagg aaaatggatt gaagtaaaaa
    22321 aataatagtt gagcttgctc caaaaacgga gtttttaaaa catccgattc tataccactt
    22381 gaataaatta tagaatttca atgatttata aacttctatt cgtgtttggt tgctgaactg
    22441 aacacagtta aagtgttcgt tgctaaattt gcttttcgga gtggactcaa aatattattt
    22501 tacaaaatca aattatctga ttaattttgc gcccatcaat cataaaaata aatggtaaca
    22561 tgaaaaaatt tacttggatt cttcttttag ttttagtaag cggcttttca catgcacaaa
    22621 gccaaaacgt gccagaggca ggcaaatttg gcggcgctga aaaacaggtt gtttataaaa
    22681 atcaagccgg atactcattg cagcacatga tcaacaacgg acattaccag atgcgtgccc
    22741 tcattgatta cgactccctc aatatgagtt ttcttggcaa ctggccttcc ggtattaact
    22801 actctttgat ctattccggt attgacaata tttttatgct tggctccggc ggcactatga
    22861 tagtactcga tttaagtgat ccggaacaac ctgaaaccat ttcagaagta agaagccgtt
    22921 ctttggttga ttgcgccttc ttcgatactg aaaccaacag actttatctt ggagcttatt
    22981 tctctggtgt cgaaatctgg gatctgaatg atatgcaaca ccccttcagg ctggcgcgta
    23041 tccctactga aagctatcca agaggcggcg tatttgctga aggcaatatt gtttatgtta
    23101 tgacagtagc cgccggaatt tacatatatg atatcgaaaa tttggaagat attaacttga
    23161 ttggccacta ccccatacca tccagtagcc ttgtttggaa ttctgcaaaa gatggtgatt
    23221 taatgttttg cgctgcagga aatgcctgca ggattgtaga tgtttcagac ccctccaact
    23281 tagtaatggc aggcgttata gctggcgctt ccgcaggggt atatgctgaa aatggtaagt
    23341 tctatcaatt atcaagcact tcaggattaa agatatggga tgtaagtaat ccagccactc
    23401 cccaattgct tggacaaata gctatcagtg gcaatcctgc caaaatcagc gtaaatggcg
    23461 attatgccta tattacaagc gctgcaaatg aacccaacgg cggtttaaag ataattgatg
    23521 tttccgaccc gacaaatcct caggaaaagt catcatttgt ttgttacacc gaacatgttg
    23581 caacaaacgg ttctttaact ttaatctcag ccggaagcga gtgtaaggta atagatgtta
    23641 ccgatattaa ttctccacaa ctaaaaagca ctatatcact tcctggatgg gtacacaacg
    23701 ttttcttaaa aggtaattac ggatacaccg gaagcaatgg tttccgcgtc ttcgacttga
    23761 gcgataaatc aaaccctgtg caggttggtt ttaatgaaac attgggcgct ttcgtagcaa
    23821 ctaatagcga ttacgctgtc tatatcccat atagcgcagg aggtaacaac aaggtaaata
    23881 taatggacat ctctgaccct acaagcccca aaaaaatggg gcattatgta tcaccagcca
    23941 tgacatacaa agtggacctt aaagacaatt atgcttttat tgcctgttgg tgggatggcg
    24001 taagagtaat taatttctcc aatcccgaat cccccgtttt agctgctcac gtgcttggat
    24061 ggtataatgg cgctatacca ggggatgaat attgttttgc acaatccatt gatgtagtag
    24121 gtgattatct atatgtaatt gactacatgc cttttgagga tcaagacact aaaggaattt
    24181 atatctttaa catatccgac ccagccaacc cggttttcat aaacagattt aaagagttta
    24241 actccaagtg taatgacatt tcagttcaag acgattatgc ctatcttgcc gataatgttg
    24301 gcggtgtgga ggtaataaat atagcagacc caaataaccc ttttgttgtt ggatacgttt
    24361 acctgcccga tgtagcagaa gcgataacaa cagaaggaaa gtatgcctat gtgagtaatt
    24421 atatattagg aggtgtacag atgctagata tttcattccc gccaaactcc aatatcgcag
    24481 gctactacaa gcgatcggga gtgtttgctc tcggggtgtc agttcaagga tcacacgtat
    24541 atatagggga tgggattgcc ggttttcaaa tttatgacaa cctgtttata acaagtgtgg
    24601 aagatctcaa taaaaacaat tcagaacaac ttgatattta tccaaatccc gctttgcaat
    24661 ctgcaaaagc taatttcata acaactacca attcctactg taatctttca atttatgatt
    24721 taacaggacg aaaagtacaa gaaattttta atggctggct cgatgccgga gaacaaactt
    24781 tcgaaatcaa aagcaaccaa ctcaaagcag ggatctattt tgtaaagcta gaaattaatg
    24841 atcaagtaag aacttcaaaa ttagtcatct cgcactaatt ggaaacatca agttttgata
    24901 taacattcga gataatatca agaatgtact gaagccttta ttgtactaat tgtaaataaa
    24961 aaacatttta tttcaagtat tattaataat cttgaataaa aaagcgttat atctttgaca
    25021 caattatcag tattaacatt attatgaaaa aatatttcat tttagcttca tgccttttgt
    25081 ttctcaatgt tattcttgcg gagaatcctc gattcataca ggcgcagctt tcctacacaa
    25141 ccttttactc gccggccgac ggaccataca tcgaaactta cctttccgta atcgggggta
    25201 gcttagaatg ggtgcaaaac gatttaggtc agtttcaaag taagctgaac gtaacaatga
    25261 ttttcaaaga cgggggcaaa atagccgctt tcgacaaata cgaactctca agtcccatta
    25321 tcagcgatag tgcggaaagg ttcatcactt tcctcgacca gcaacgattt tcgcttccta
    25381 atggtgaata tgactttgag attactttaa aagacccaaa cgcagatact cctcccttca
    25441 gcgctaatga gactatcatt ataaatatcc caaaggatgc tgtatccatt tccggcattc
    25501 agcttttgga atcctataaa aaatcagaag aacctacagt tttaacgaaa agtgggtacg
    25561 acatgatacc atacatttat aattttattc ctaaagaggt caacaccata aacgtctatg
    25621 ctgaaattta taatactgac aaagtcttcg gcacagatca gaaattttta ctgagtcact
    25681 atattcagtc ttatgaaaca aaacgcacga tgagtagttt ttctcacttc cgacgtgaag
    25741 agagcaaacc ggtaaatgtt ttactggtca actacaacat cgccgaactt ccttcgggca
    25801 attataacct tgttattgaa gtacgcgaca gagaaaacat gttggtaaac agtcagaaat
    25861 tatttttcca gcgaagcaac ccggacttaa aaatcgagca gcctatctat gcaagtatct
    25921 cgatagaaaa ttcattcgta aataaaatca ccaatcaaga tacacttact gaatacatta
    25981 aatttctcac ccctattgct actgaaatag aaaaccggtt catcaggcag caaattgaaa
    26041 gtcaaaatgc cgacttgcaa cttatgcagc gtttttttct caatttctgg cttgaacgtg
    26101 acgaagcaaa ccctgaatta gcctggaaca catatttagc caaggttaat ctggtaaacg
    26161 aacaatttgg ttcgccaagt aaaaaaggta aaagggggta tcaaactgat atgggatatg
    26221 ttttcctgaa atatggtcca cctaatacaa ttaccgatag gccttttgat gccagtagct
    26281 cagggttaac cattaaccca ggtggtgaag agactattgc gatggggtcg gtgccatacc
    26341 agatttggca ttattatagc atcaacgaat accgcgacag gaaatttgtc tttgctaacc
    26401 accatctggc gctcttcgat taccaactta tacactcaaa tatgccgggt gaagtacaaa
    26461 atgacaactg gcaaagcgag ctgttctatc gttttaatgt agggggtaca atgcctgaaa
    26521 cagacaaata taaaggacaa tcaggcgact tttacaacca tccccgctaa tcttttaatc
    26581 ttcaaaaacc gatcgttgag tatgccaatt tgtaatcaaa aatcggttta caatttaata
    26641 tttaccgatt ctctcaatac caaaggaaaa aaagctattt ttatccgcta aatgaacatt
    26701 tcaattgtca tattaaactg gaacggaaaa aaataccttg gcaccttcct gccaaaacta
    26761 ataacctcct caccggaagc caatatttat gtggcagaca atggatcgtc ggatggctca
    26821 gtaagatttc tacagcaaaa ctatcccgaa ataaaagtta tcccttttac tgaaaatcat
    26881 ggttttgcta aaggatataa ccttgcccta aatcagatcg aagctgacta ttacattttg
    26941 ttaaataccg atatcgaagt taccgataac tggatacagc ctgtaatttc ggtgatggag
    27001 caagatgaat ctatcgctgc gtgccagcct aaaattctat cgtacgacaa tcgccgccat
    27061 tttgaatatg ccggagctgc cggaggcttt attgaccgat acgggtatcc attttgcagg
    27121 ggaaggattt ttcaagtaat tgaagaagat gttggtcaat acgacgatat tactgaaata
    27181 ttttgggctt caggagcttg cctattcata aagtcaaacc tttttaaaaa agccggtggc
    27241 tttgataatg actttttcgc acacatggag gagattgacc tttgctggcg gttaaaacat
    27301 ttaggataca aaataatgta ctgcgggcaa tcaacagttt ttcatatagg cggtggcacc
    27361 ctcgacaaat cagctcccaa aaagacatac ttaaacattc gaaacaataa catcttgtta
    27421 tacaaaaact taccctcaaa acagataata ccaaccttca tagcacgctt ctttttagat
    27481 ctgacggcat cattaaagtt tttaattgat ggaggagtcc ggcattttat tgcagtatca
    27541 agagcccata taggttttta tttctcacta agaaaaaatc acttgaaaag aaaaaaaata
    27601 aagcatatta aggtttcaaa aatttatttg ggcaatatcg cctttgacca tttccttttc
    27661 ggaaaaaaaa catttaaaca gttgaaatcc aagatgttta catgaattta aaatgaataa
    27721 aactaaagca aatgataaat gaaggaatca aaatttttta aaattttgtt ttttatttgt
    27781 tatttaaaaa gaatgattac ttttgtaacc gcatttacat tgctttacaa tccttaaccg
    27841 cacaattaaa ttttcacttt cacgaggaat tgtgtaaatc atcactaata aaaatgttcc
    27901 atcttaagaa ttctcatttc aaaaactaat tattaaaaaa aacaaatctg ctcatacaag
    27961 cagtattatc accccaaaca cataactaaa tcttttttta atccgaaaat ttaacttttc
    28021 aatttaatgt acgatataat tgaacttaat gcgaaactac ttcctgatct tcgcgaaata
    28081 gccaaagagc ttaatatcac caaaatcaat aatctgaaaa agcaggatct gatatataaa
    28141 attcttgatc aacaggcaat taatccccca aaatcagaca catctaagaa gagcgatcct
    28201 tttttcgata aaaatcaaag acgtaggtta caatcgagtc tgataaaaga aaaaatagca
    28261 tcaagctctg atagtagccc gagtgtagct gttagtgggg ttacacatat tacaccagag
    28321 gccaagcctg ttacctcact atccgaggag aaaaaaagtc agaagcaatt cccgcacgat
    28381 aaaccgagga agcaaaaaac agatcataaa gtccaaaaag ccgaacagga aaaccgtttc
    28441 aataaatcat cggctaccga taaacaatct tcgcccgacg atgacaccgt tgatcagaaa
    28501 ttagaagaga ctcttattgt tcaaaaaaga acacatgttt taaataaaac caaagatctt
    28561 tataagaaac cagaagttgt cgtaactaac cttgctgatg atcaaaaaac tactgaggca
    28621 aaacaaagtc aggttgccac aactgctgaa cagcaacggc atagtgttaa cgatagacaa
    28681 cgtgaaaagt tttcttttga aattgatggg atcgttgata cagagggagt tttggaaata
    28741 attcctgaag gatacggatt tctacgttcc tccgattata attacctcaa ctcgcccgat
    28801 gatgtctatg tttcacaatc gcaaatcaaa ctgtttggat taaaaaccgg agatacatta
    28861 agtggtagtg tgcgcccgcc taaagaagga gaaaaatatt tccctcttat aaaaattgaa
    28921 aggataaatg gccgcgaccc caatgaaatc agagatcgga taccttttga ttacctaact
    28981 cctctatttc ctgagaaaaa attcaagctc accggacatc cgcaagagac gatctccacc
    29041 agaatcattg atatgttcac tcctatcggc aaggggcaac gcgggctaat agttgctcag
    29101 ccaaaaaccg gtaaaacagt gttgcttaaa gacatagcca atgctatcgc ctataatcat
    29161 ccggaagtat atatgatgat tcttctaatt gacgagcgtc ctgaggaagt tactgatatg
    29221 cagcgaagtg taagggcaga agtaattgcc tccacatttg acgagccggc cgaaagacat
    29281 gtaagaattg ccaatattgt gctggaaaag gctaaaagac ttaccgagtg tgggcatgat
    29341 gttgtcattt tacttgattc aatcactcgt ttggccagag catacaacac cgttgctcca
    29401 gcatcaggaa aggtgcttac aggcggtgtt gattcaaatg cactgcaaaa gccaaaacgc
    29461 ttttttggcg ccgcacggca aatagaacac ggcggatcgc tcaccatcat ctccacggca
    29521 ctgattgata cgggttcgcg aatggatgag gtgatttttg aggagtttaa aggaacagga
    29581 aatatggaac tacagctcga caggaaaatc tcaaataaac gtatttatcc agccattgac
    29641 attgtagctt caagcacacg tcgcgacgac ctattgttag acaaagagat gctgcaacgg
    29701 gtctggatac tcagaaatat cctctcggaa agaactactg tagaggcaat ggaattctta
    29761 aaagataaaa tgcgttttac acaaacaaat gaagagttcc ttatctcgat gaatagttaa
    29821 gtataaaaat ttaagaaaat cattaggatt tcaaaaaagt attacttttg cagccctaaa
    29881 atcagaaaat gatcatatca tgtcgaagaa aactaaagat gtaaggattc aggtaatttt
    29941 agagtgtacg gagcacaaaa acagtgggct tcccggaaca tcgagatatg ttaccatgaa
    30001 gagtaaaaag aacacacccg agcgtttaga gttgaagaag tacaacccta tcatgaaaaa
    30061 agttaccgtt catagagaaa ttaaataata aggagacaaa gcaatggcaa aaaaagtagt
    30121 tgcaacccta aaaaccgcta caggtagaga tttcacaaag gtaatctcag tagtaagatc
    30181 agcaaaaaca ggcgcttaca cctttaagga agagatgatg cctaccgaaa gagcacagga
    30241 actgattaaa aaaagataat aatcccctga aaaaatcttt tagagccttt tcctgtatag
    30301 gagaaggctc tttgtgtttc atctaattaa tttgaaaata tgggtttatt tgattttttt
    30361 tcaaaaaaga aaaaagaaca gctaaatgaa ggtttggaaa tgacaaaaaa gagcgtcttt
    30421 tccaaaatca ctaaagctat agccggcaaa tcaaaagttg atgacgaagt tcttgacaac
    30481 cttgaggaga tacttgttac ctctgatgta ggtgttacta ctactttgaa aattatagag
    30541 cgaatagaag caagagtagc gcgagataaa taccttgggg ccgatgaact gaacagcatc
    30601 cttaaagaag agatcgctgc cttattatcc gaaaatgata gtgatagtat atcaggtttt
    30661 gattttccaa aaatggaggt tccttatgtc atcatggttg ttggagtaaa tggtgttggt
    30721 aaaaccacca ctattggcaa gctggcatac cagtttaaaa tggcgggcaa atccgttttg
    30781 cttggcgctg ccgacacctt tagggcagca gccgttgacc aattgaccat ctggtcggaa
    30841 agagttgatg tgccacttat ttcgcaaggg atgggcgccg acccagcctc agtagcatac
    30901 gacacgttaa aatctgccgt agccaataag gccgatgttg taataattga tacggccggt
    30961 cgtctgcaca ataaaataaa cctgatgaac gagcttacca agatcaaaaa tgtgatgaaa
    31021 aaaatcatcc ctgaagctcc tcaggaaata ttactaatat tagatgcctc caccggccag
    31081 aatgccattg agcaggctaa gcagtttacc gccgccaccg aagtaaacgc attagcgctt
    31141 accaaacttg acggcacagc caaaggaggc gtagtcatag gaatttcaga tcaatttaaa
    31201 atacccgtta aatacatagg tatcggagaa aaaaaggaag accttcagtt gttcgacaag
    31261 tttgagtttg ttgattctct ttttagtcaa gagtaaaaag aaattatttt gaaaacaaaa
    31321 aatggtaagc catcagtaac aattgtaaca ttggggtgtt ccaaaaacct tgttgactct
    31381 gaagttttga ttcgtcagct tcaggctgga ggcatcgagg tttcgcataa tcagcaaaat
    31441 aacatttcga aaagagtaat tataaacacc tgtggcttca tcaacgatgc aaaagaggag
    31501 tcaatagata ccattttgaa ttttgtaaac gccaagcagc agggaattgt tagtgaattg
    31561 tatgtgatgg gctgtttgtc agagcgttat cgccacgagt taagcaaaga gataccagaa
    31621 gtagatggtt tttttggagt atcggagttg caaaacatcc ttcgtagcgt tggagcagat
    31681 taccgaaaag agcttttagg cgagcgacat ctcacgactc cgaatcatta cgcttatctt
    31741 aaaatttcgg aaggatgcga cagaaaatgc tcattttgcg caataccact gatacgcggg
    31801 aaacatcaat caaaaactat cgaagagatt ctgaatgaag caaaatatct ggcctctgta
    31861 ggagtcaagg agttaatcct cattgcgcaa gacaccactt attatggttt ggatatctat
    31921 ggtaaaagga gacttcccga tttactgcaa caattaacct tgatcaacgg tatagagtgg
    31981 atccgcttac actacgccta ccctaatgca tttccagaaa agactttaag cgttataaat
    32041 tcatctccga agatttgcaa atacttagat attcccattc aacacatcag cgatcctatt
    32101 ttaaaggcaa tgaaaagatc gcactcgaac ggatacaccc gaaaactaat agaaaagatt
    32161 cgaaatcaaa ttccgggagt ggctctacga acaagcataa ttgttggctt tccgggtgag
    32221 acaaatgaac agtttgacga attaaaaaac ttcatcagcg aagtgaagtt cgatagactt
    32281 ggcgttttca cctactccca tgaagagaat accaaagcct ataaattaaa agatgatatt
    32341 tcggctgcaa caaaaagaaa aagagcgtcc gaacttatgg cgttacagca acagatttcg
    32401 tttgatatta accgaaaaaa aattgggact tcgctgaaag tgatcattga ccgtaaagat
    32461 ggagatttct ggatcgggcg atccgaattt gactcacctg aagtagataa cgaaatacta
    32521 attccatcaa cctttccatt aaaagaggga agcttagtta aagtaaaaat tactgacgcc
    32581 gaagccttcg atctatttgc tatgccagta taaaatcatg ctaccaccga tatttccttt
    32641 ttgttgatta tttttgcaca ttaaatcaaa aaacatcaga catgaaaaat ttaatgattt
    32701 tattttcagt aatgtggctc tcagtatcat gcagtttttc tcaggaacat aaaaaccaac
    32761 caactgagaa tattaaccaa accgacagaa aggggcaaaa gcagggatat tgggaagaga
    32821 cagaggacaa aataaaaagc gtcgggatat acgccaacgg taaaaaagag ggctcatggc
    32881 tcacctatca tccatcaggg attgttcaac ttgtggaatc atataaaaat ggaaaaaaag
    32941 atggggcttt tattgaaatt gacagtagag gctattttac tgaggagcaa ttttacagta
    33001 acgacaaaaa gcatggtaaa cacagggttt acgaacgcgg ttcgcgcttg gtactcgaag
    33061 agaactataa taatgatcaa ctcgacggca ccaagaaggt attttatagt aacggcactt
    33121 tgcaagagga atcggaatat aaaaatggcc agaaaaacgg gacttcaaaa tggtttaatc
    33181 agtctgggca aataattgca gagtataatt atgtaaatgc caaattcgaa ggcacacaaa
    33241 agacctttta tgagaatggc gtactgatgt cggaagagac cttcaaagag aatctgcgca
    33301 atgggtctgc caaagagtat catagaaacg gaaaattaat gaaagagggc gagtacaagg
    33361 ataacctgaa agagggcaaa tggctcgaat atgatgagaa tggaaatttg ataaatgaaa
    33421 tagaatacgt taaagatgaa caaaaataac gaacaagtat ttcgaaaaat cagaaatcca
    33481 tttactatcc ataaaggttt caactgtttt ggatgctccc ccgacaaccc cataggatta
    33541 cagctcaact ttattgatca cggcgatttt attacagcca actggcagcc acaaactaat
    33601 tatcaaggtt atcacaacct tctgcatggc ggaatacaag caaccttgct cgatgagatt
    33661 gcaagctggt ttgtaaatgt aaaactaaaa accgctgggg ttacttcaaa gatagagatc
    33721 aaatacaaaa aaccggtgta cgttgactca ggcccgataa tgctcaaggc tactctaata
    33781 agattaatgc gaaatattgc agatataaaa gttgagttgt ttaacgatga aaaacaatta
    33841 tgtgctgttg gagttgcaca gtatttcacc ttctccgaaa aaattgcaaa agaaaaatat
    33901 tactacccgg aacctaataa attctatttc gaaaaggatt aaatcaaagc ccaaatcata
    33961 actggctaca tagtttttcg aaaaaagaga cccaatcaac acccgaattc ctcttcccta
    34021 acctgacgat aattacctcc ttatcaggga atacatagat atactgcccc aatattcctt
    34081 tggcaaaaaa agcgccatcc tcctttaccc tccaaccgta tgaatacgga atcccttgtg
    34141 aatcgcaaaa atttccatcg gctttcatgg ttgcctcaat ccatctcttc ggaactacct
    34201 gcaaaccatt ccagttacct ctattcaagt aaagtcgccc aaattttgcc atatccaacg
    34261 ccctactatt taaacaacaa aaggctttga tctgacctct ctttttacta tctaaactcc
    34321 acgttgcgtc atactccatt ccgaggggcc tccataatct ctgttctata agctcggcca
    34381 gatttccacc ggtaacacgc tccaacacaa taccaagcag caagctatta acgctcacgt
    34441 aatcatattc aaggcctggc gctgatttga tttttaatct accgacatac ttttttaaat
    34501 ttttaccgta ataatatttt gccatatcgc taaaaggggt agaatagcct tcgttaaacc
    34561 gtattcccga ttgcatattg agcaaatgtt caagggtgat tttcgaaaac cggatatcgg
    34621 agttaagaag ttcggggata aaaccggtta cagactgatg tacagattta atgtatcctt
    34681 cctctataat aatcccgaca agagccgaca caaatgattt gctgaccgag aaagacgaaa
    34741 ccattgcatc ggcctgaaaa cccttcgaat aatgctcgaa ttgcaaagtg tcattcttta
    34801 taataagcaa tgcgactgtc ttggtatttt ctaaaaaatc aataaaacta ttttcaaaac
    34861 caccttcgca aaaaccttcc gggaggttca acgaaatgaa agaatcggaa ggaataaagt
    34921 gaaaagcagt atcgcctttg ttaatagttt cagcaggaaa cttcttgtaa tcatcaaaat
    34981 cagccagatt ccaataaaaa tacctcgcaa catgacacga gtttaacgtc aaaataagtg
    35041 gtaggattag tatgatgatt cgatttttct tcattttagc aaaagtattc aaaacttcag
    35101 tttgcttttc aacttgaatt aatcagaata atgctttcat ccgctaaact attgatttta
    35161 tgaaactaat ccggaattat tatttggtcg gtagaatttt tacagcgctg attacaaaca
    35221 tataattata aaaatactgc cctatgaaaa atcggtttga aatcacatca gataattttg
    35281 aaccgtctaa cattttgcag cctggaggag taacggatat atccatgtct tttatcaatc
    35341 gttacattct ccactgttac cgacgctttt atttgatata acgaatggct attccaataa
    35401 aattttcccg tttttacaac atcaaattgg tcatttttaa aagaaaaaca gtttgtttag
    35461 tactaattcc tatttttgtt ttttaattac gattaataaa caaacatcaa tatcatcata
    35521 tcatggcaga tttaagtact aattacatgg ggtttaagct taaaaaccct atcattgccg
    35581 gtagcacagg gctaagcatg agtgttgaaa acctcaaatc acttgaaaaa catggcgccg
    35641 cagccgtagt tctgaaatcg ttgttcgaag aacaaattat gcacgaggta aacaaagctt
    35701 tagccggcac tgataacaaa gatatttcat actaccctga agctgaggac tacatcagac
    35761 attacaccaa agagaaagat ttgaacgact acctccgtct gatcgaaaat ggaaaaaaat
    35821 ctataagcat tccactgatt gccagtatta attgcttctc ggcctcggaa tggactacat
    35881 ttgcaaaaaa gattgaaagc gccggcgccg atggccttga gctgaatatt tttatcctcc
    35941 cttccgacac taaccgtgag ccctcccaaa atgagcaggt ctattttgat gtagttactg
    36001 aagtgctcaa acaagtttca atacctattg cacttaagat aagttattat ttctcaggac
    36061 ttgcaaagac agcgttaaag ctctcgtgga caggcatttc cggtatggtg cttttcaatc
    36121 gctttttctc gcccgacatt gatattaaca aatttatagt aacgccttcg cacgtattca
    36181 gcaccaaaga tgagatatac actaccttac gatggattgc catgcttagt aacagactac
    36241 attgcgatat tgccggatca acaggcgtac acgacggcga aggggctgtt aaactcttgc
    36301 tggccggagc taaagcggta caaatagcaa cgatacttta caaaaacggt tttgaaacca
    36361 tcggcgaaat gatacgattt attgaacagt ggatgaataa gcacgatttt aaaactaccg
    36421 aagagtttat cggcaaatta agtatgaagt caactgaaaa tcctgctgca tacgagagag
    36481 ttcagtttat gaaacacttc gcaggcatct attaacatca tatcaacaca ggtcggcggc
    36541 gaggcgactt gaaaaaatca cgaaactcgg gcgggccggt aagatactta ccggtaaaat
    36601 cgtccatatt atcaatcgtt tcattagaga gcaagtcagc aacatcgtcc atcgaattag
    36661 cgccaataag aaatggaggc tcgtcaagtt tcaaaaaacc acccctaaag cgcccaatga
    36721 tctgatactc ctcaagctca gtaactttct tgccagtaat agcacaggtg gaaaggtgat
    36781 cgtaaatatc aatttcggaa gtgatagatt tttgaaaaat atgggtaata cgaccttcca
    36841 aatgttcagc aaaataacgc tgcaaattct ctttcgattg agctgagtaa ctatttacaa
    36901 aagtaagcgc acaactgaaa catatagcaa attcaaacat cacctccatt ttgccagata
    36961 tgggattacg tctaaacacc ttttcaatca tgtactcagc atcagtttcg atgatctttt
    37021 tctgacagac ctggcacctt tcgataggca tattgctgat actgtcataa aacagcgaag
    37081 ggataatcaa tttagaatca tcattgtagt agttcgtagt ttccataatc aatcatttca
    37141 gttaaacaag catcctcaat cctgataacg gataaaataa tgaaatcaat cggttttgga
    37201 tcattttaat caggctattt cttttgcaaa ttaaaaaaat cgcaaatcaa aaatcaagat
    37261 gttggatttt ataattttga aaactatata acttgcgcaa ataaaccatt attttgcata
    37321 acgaaataat taatcatgat aattgaaatt gatccggggg ctggtttttg ttttggcgtt
    37381 aaaagagcta taagccttgc cgaagataag ctacaacaag gaggtaagct gttttcgctc
    37441 ggacaaatcg ttcacaacaa agacgaagag caacgattga atcaacttgg catgactacc
    37501 attacacacg atcaaatgga tgaggttaaa gataatccca tcctgataag agcacatggc
    37561 gaaccgaaat caacttatca aaaagctctt aaaaacaata tcgaactgat tgacgctacc
    37621 tgccccatag tattaaaatt gcaaaatgaa ataagaaaat gttggctcga aggtgatgaa
    37681 gacaaccggc aaatcgtggt ctttggaaaa aagggacatc ctgaagtgat tggccttcag
    37741 ggaaatattg acaatcaggc aattattatc gaaaacattt cggatattga aaagatagat
    37801 ttctcaagac cgatatttct tttttcgcaa accacggctg acgaagaact gtttgacatt
    37861 ataaaaaatg atattgccaa gcggcttaat gatgctcaga aaagctcaac accgcctttg
    37921 catgtaacaa aaagcatttg tagtcaggta aaaaacagaa aaaatcaggt tatcacattt
    37981 gcatcgaagc atgatttaat tatctttgtc ggtggtaaaa actcttcaaa tggtcgttat
    38041 ctgttcagtt tgtgtaaggc tgccaatact aactcttttt ttatcgaaaa cgaaacaaat
    38101 atcaatcccg gatggttgac tggggtaaaa agcattggga ttaccggcgc cacctcaaca
    38161 ccggactggc ttctggaaaa agttaaaagt catataaaca cgcttattca gcgctgatgt
    38221 acataaaaaa actcgacata cataatttca aaaattacga aaaggcatct ttatcattct
    38281 cgccaaaagt aaattgcttt ataggcgaca atgcggcagg aaaaacaaac ctgctcgatg
    38341 ctatttacta cctatccttt tgcaagagct acttcaacgc tgttgataca caaaacatac
    38401 ggcacgatca ggagtacttt tccatcttag gcgaaatgga tactgacacc aataaaaccg
    38461 acttaatcca ctgtgttcaa aaacgaaatc agaaaaagag ttttaaactc aataaaaagg
    38521 agtatcaacg acttgctgat catattggaa agtatccgat agtgatgatc tcgccttacg
    38581 accgcgatct tatcaatgaa ggcagcgacg tccgccgccg gtttattgat agtataataa
    38641 gccagttcaa ctctctttat ctcgataatc tgattaatta taacaaagcg ctcgcacagc
    38701 gcaactcgca actcagggcc tttagcgaac aaaattattt tgacagttcg cttttggaaa
    38761 tctgggatca tcagcttatt gaccttggaa atcaaatatt tcctgaacgc caaaagttta
    38821 ttactgagta tcagccactt ttcaataaat atttcgaagt ggttaccggc aaaaaagagg
    38881 cagcaacaat tgaatatgaa tcgcagctca acacccacag cttcgaagaa ctccttgctt
    38941 ctaccattga gcgcgacaag gcgctaagat ttactaccgc aggaactcat aaagatgacc
    39001 tgatattcaa catacaagga catgctataa aaaaattcgg ctcgcaaggc caacaaaaat
    39061 cattcatagt tgcactacgg cttgcccagt atatgtatat taaagagttg aaaggatata
    39121 aaccgatgat ccttttggat gacatctttg ataagcttga taacaaacgt gtagaacaat
    39181 taatacaatt ggttgcagag aatgctttcg ggcaagtttt tatcacagac acgcagcaag
    39241 atcgaataga atatctactc aacaaaatct ccaataacta tcgtattttt gaggtgtgta
    39301 atggtactgt aaacgaaatt ataactcttt aaatccgtca gacgatgtca ttcagagtac
    39361 ccaacgaaca aaccttaggc acagtgatac gcgaactgtt ggtaacactt cagatcgagg
    39421 acaagcttaa agagactcaa atgatcgcct cttgggaaga gatagtgggc aagatgattg
    39481 ccaaacatac aaagtcgctc cgaattgtaa aacgtgttct gtatgtggag gttgactctg
    39541 ctgctttgcg taacgagctt acatatttac gtgaaaaaat taagaaaaag ctgaataaaa
    39601 aagccggcga caatttaatt gatgaggtaa ttatcagata gctgaataaa aaacaaaccc
    39661 tctatcagct tttattttta tcccgaattg gcattaaagg gcgcttttaa tcggaacggt
    39721 tgatgttaag agaacaggcc aatattagtc tgtgatataa ttctacttta tcatctccat
    39781 aaattcagct tcgctgataa tctttactcc aatagcttcg gctctttgta ttttagccgg
    39841 acccatttta tcgcctgcaa caagaaaact tgtgttggcg ctaacggcgc ttaataccct
    39901 cccaccctgc tcactaataa cctgcttaat accttcgcga gaaaaattct gaaatacacc
    39961 gcttacgaca aacgacaatc caactaactt tccgtcagca attggctctt cagcctggat
    40021 cgttttcatt tggatcccgg catcttttag cttagcgata atacgaatat tttcaggttt
    40081 cgaaaaataa tctgtaatac ttccggcaat cctttctccg atttcgggaa cagatgtcag
    40141 tgtttcggta tttgccatta ttaaagcatc aatattaccg aaatgtacag caagtttccg
    40201 agctaccgtt tcgccaacat acctgattcc gagagcgtaa agtactcttt gaaaaggcac
    40261 ctgctttgag ttttcaatgc ccgtcatgat gttttctact gtcttctcac gaaagcttac
    40321 cctacgctcc tttttttcgc catcggcagg aaatattttt tctaaaccaa gcaaactctc
    40381 gttcgacaga taataaaaat ccgatattga tttaacaagg tcattttcat ataaaatttc
    40441 aatccttcct tcacctaagc tttcaatgtt catagccttc cggcttataa aatgctcgag
    40501 cctacctttt atctgcggcg ggcaaccgct atcgttcggg caataatgag cagcctcgcc
    40561 ctcattacga atcaaggtag cgccacattc agggcactgc cttataaatt cagtagtttg
    40621 tgaagattta ggtcgtctgt taaagtccac gccagtaatt tttgggatga tatctccccc
    40681 tttttcaaca aacacaacat cgcccaccct tatatcaagc ttttctataa tatcggcatt
    40741 atgtagcgaa gcgcgtttta ccgttgtgcc ggccagcgtt acaggcgcca gatttgctac
    40801 aggtgtgatc gcccctgttc tgccaacctg ataatcaata gatagtaatt cggtagcagc
    40861 gctctccgcc tcaaatttat aggcgattgc ccacctgggc gatttagctg taaacccaag
    40921 agcttgctgt tgttcataac tatttacctt tatcactacc ccatcaatat caaaattgag
    40981 atccctacgg gcagtgttcc aatattgtat aaaatcaaaa atatcttcaa cagaactaca
    41041 ctttgctatg taatttggaa ttttaaaacc ccaacgtctg cactctctaa ggctatcata
    41101 atgattattt atcggaagtc tatcgccaac cagatagtat aaaaagcaat ccaaactacg
    41161 ttttgccacc tcagcggaat cctgcatctt caggctgccg gctgctgcat tgcgagggtt
    41221 tgcaaaaggc tgttcgccaa tctccactct ctcctcattt atttgatcaa agacgtctct
    41281 ggtcatcatc acctcacccc tgatctcaaa cttttcggga taaccatcag cacgaagtct
    41341 caacggaatg cttcggatag ttttaatgtt ggttgttaca tcatcgccct gttcaccatc
    41401 gcctcgggtg atcccactaa ccaagcggcc attctcatat cgcaagccaa tagctacgcc
    41461 atcatatttt aactcgcaaa catactccac cttctccgga attagctttt tcacccgagt
    41521 cacaaaatct ataatctcct gttccgaata agtattacca agcgatagca ttgggtattc
    41581 atgtcttacc tgtttaaact ctttggttat agcccctcct accctttgag tgggcgaatc
    41641 agaaaaggca agatcaggat attgcttttc aagctgctcc aactgcttca tcttcatatc
    41701 aaaatcataa tccgagatca acggctgagc caatacgtaa tatcggaagt tatgttcatt
    41761 tagctctttt gtaagctgat caacaaggtc tcttgctttt tcgaatgtca tttttcgata
    41821 aattatggtt caaaactaat ctaaatgaat agaagcttat ctgattaaag tattaaaaag
    41881 tttaagtttc aagtaatttc ttgccaattt ccggtaataa caatattcgt tttgatacta
    41941 acgccgtggt gtatcataac actcttttcg gcctcaagta tatcgttcaa taaagcctgt
    42001 attaagcaga gagagcaggg atcgaacctg ccacagccgg ttttatccgt ctgctattgg
    42061 ttttgaagac caaccgggac accagtccca tcctctctta atatttttgc gacaaagcta
    42121 ataaatacta taaaacccat catcctacat tagctccgta gtttatattt ctttcttagg
    42181 ttttcattgt tttttttggt ggttagcagt tattaattag ttttgtgttg ttggcgaaat
    42241 ggtaaaaaaa gacgaattgg aaaaggcgat ctggttttgc gaatacataa aaggcaacac
    42301 tgcgaagcct atgcaagccc catcaaaacg atgattcgaa aaacagagaa aagaatttta
    42361 gctttacccc caaaaattaa caagtcattt ttaaacattg atcaaattaa aaatggagaa
    42421 taaaagacaa aatcgttggt tggtagccat catgggcacg cttttaatga ttgtgcttgg
    42481 gactgtttat tcatggagtt ttttccaaaa gccgatagtt gagacttacg gatggtcaaa
    42541 ttccggtgta gcctgggcat tcagcgttaa tatattgtgt ttgggcttag cggctgcatg
    42601 gggtggtgtt tatatgccaa aatatggacc aactaaactt gcaactatag gagctataat
    42661 gtacggcgtc ggttttatgt tgggagcgtt agccctaaaa atggaaaacc tcgcctttct
    42721 tgttattggt tttggaataa caggcggact tgggattggg ctcggttatg ttacacctgt
    42781 tgtttcagct tccagatggt ttcccgacaa acaaggtttt gtaaccggca tggtcgtaat
    42841 gggctttggc ttcggagcgt tagtaatgtc gaaaattata gctccagggg taatgaaagc
    42901 tgtggaaggc aatttggtat ctatattttt ccatatcggc atcatcattc ttgtgcttgg
    42961 atttattgca gctatcttca tcaagcaacc cccagccgat tatgtacctg aaggatataa
    43021 acctcctgta aataaaacaa ccgacaaacc tgtaaccgtt gatattactc ccttacaagg
    43081 tataatgacg tccaaatttt tccttatgtg gatgctattg tttatcaaca taactgccgg
    43141 tattatgttt atcagttttc aaagccctct tatgcaagac ctttgggaaa ttttcaaacc
    43201 aggcgccgat gcggcctcgc ttgcagccat tggcggcacc ctgattgcaa tcagttcgct
    43261 atttaacggc atcggcagac ttttctgggg aggcttatcc gataagatag gacgcgtgca
    43321 aacattccgg ctaatacttg gcacacaaat aattgttttt ataatacttc ttttcgttaa
    43381 aaacccatat ctcttcggta tgcttgtttg ttacgtactg ttgtgctatg gtggcggttt
    43441 cggtacaatg ccctcattcg tcagagacgt attcacccca aagctgatgt cggttgtata
    43501 tggtgttatt ctaacagcct ggtcattagg cggtatagcc ggccctcaga ttgtagcttt
    43561 tatgaaagac aactacccga caaatgccgg acattattca gtcatcgcgg gagctatatt
    43621 gttggcaatc ggtttcattt tgtcattact ggttaataat aacatcatca ccgacaaaag
    43681 ataaacatca actttattaa atcaacaaaa catcaagact gtcttgatac aaatttatac
    43741 ttcaacctac tttaataaga cacaaacagg aataaacctt ttagaatgta agttagttat
    43801 tcaacagctt ttcgcaccgg tatccttatt gttgatgttg ctgataaaca cttatctatt
    43861 tagggacttc tttaattctt atcaaataaa atgttttatt tttacttttt tttatcaata
    43921 gtcataattt aaaaactaaa aaaaatcatt atgttacgta aagttttggc attcatcaca
    43981 tttgcaatta tcctctcatg gcaaacagtc atagcacaag atgtacaaaa agaggaaaac
    44041 aaaaagttac agttaacgcc ttatgggttt gtcaaaggcg acatgttcta tgttaccgat
    44101 ggcgtttact catttgcaaa ccccaacaac gattatattc ttgcacctca gtttgccagt
    44161 ggtagcggcc agtcggctat tagttttaca ggccaacata cccgccttgg gctgaaaagc
    44221 acagttggta acaaggtaaa ggttggcgga ttagtagaga ttgacttttt tggcgggcct
    44281 gcagatgcta acacaaaaat tcggcttcgt caaggctatg cctctattgc taccggaggt
    44341 tttgaagcaa ggtttggaca gcagtgggac ctcttttctc ccaatattcc aaataccgtg
    44401 aatacaaatg ggaacatgtg gtacaacgga aatatgggat ttcgccgtgg gcaaatacaa
    44461 ttaagctaca agtggaatct cgaaaaaata aatccaatgt tgcaaatagc ttttgctgaa
    44521 ggagcgcaag aatccatagg attaggaaaa gataatatct ccggacaacc catgattcag
    44581 gcaagattat caggaaaatt tgatgacaaa tacaccgtag gggtttatta tgttaatgct
    44641 gcatttaaag agaaatatac accagcaata ccacctaacg atcatttcaa atttaacacc
    44701 acaggctttg gagtggattt caacctgcca tttgataaat actttagcct cgtgggagaa
    44761 attaacagcg gtacaaacct cgctaatgct aatatcttca gcgctgcagg aaatttcttc
    44821 tattttggaa catccgatca aacacgcatt gattttgatc gtaaaaacac aggattctgg
    44881 cttaatgctt cctccaacgt tagcgaaatg tttcaggtta tccttggata tggaatggac
    44941 aaaaatacaa gcaagaaatt cattactggc aacatccaaa gtaatagtac cttttatgcc
    45001 gacctgattt tcccaataaa acaccagttt tcaattacac tcgaaatcgg taatatctca
    45061 acgaaaatag tggaaagcat taataacgat cataaagttg ataaaactaa aacaaacaat
    45121 gctaccgtca ttgctttatc aggcaaagta gccttttaat tcgatttatc cggatttatc
    45181 tcagactcaa gaataaactg agtgtatcaa actaaaaatc ccttattgca tggagaaatg
    45241 tcattagggg gttttttgtt ttgcaatgac aaatcatggt cattatcagt aaacatgatt
    45301 agcattatat cactttgtaa ataactttat ttttgcatta taaataatca gaaaactgaa
    45361 tgaacgaagc aaaaaaatct ttaaatagga aaattgcaca tcaggaaggt tggatttcca
    45421 tcattgtcaa cagcgcactt tttgtattaa aatattgggc aggagtagtc tctggctctg
    45481 ttgccattat tgccgatgca tggcacaccc tttcggatac gtttagctcc attattgtaa
    45541 ttgcaggggc caaactctcc agcaaacctc ccgacaacga acatcctttt ggccatggta
    45601 gagctgagct gatagcagcc gtcctggtgg ctgctttcct tgggtttgta gcttttgaat
    45661 ttatacaatc ttcaatcatt aaactaaaaa accgtgaaat ggctgtgttt ggagcgctcg
    45721 ccataattgc aacggttaca tctatcattt taaaagaagg acttgcacaa tatgcctttt
    45781 atacatggaa aaaaaccggc tacctatcgt taaaagctga cgcctggcat caccgctccg
    45841 atgcaatttc atcagtaata gtgcttatag gaatttttct gggtaagtat tactggtgga
    45901 ttgatggcgt tctcggtatt ctcgttgcac tgatgattgg atatgccgcc tacggaatac
    45961 tcaaaggcac cattagtaaa cttttaggtg aagcgcctga caaacaattg attgaacaga
    46021 taaaaacaat tgccagtcaa gaaacaatac acgatataca gctacaccat attcatctac
    46081 acagttatgg cgaacatcag gaattaactt ttcatatccg acttccaaac atgatgactg
    46141 ttgaggaatc gcatgcgctc gttactgcta tccgtcaaaa actacgcgat aacctatcaa
    46201 ttgaagctac gatacacatt gaaccttacc aaaggcaata aagtaaaagc taatgctttg
    46261 tattaagaat tgcgtcagac tctttttaaa atccccttct tacaataaat cctgtctcta
    46321 ttcataacct agccaaatat ttctcaaccg ctgccatcgg tggatcagaa acacacaaat
    46381 agtagcaact cacactaaaa tcaatatttt tcttaatcag atatcaaaaa aaattacctt
    46441 tgctgtatca ataaataaag atgaaaagaa cctatttatt agttttcctc tcaacgttga
    46501 ttatttactt aataacaagt tgtagtggga atttagatcg aaaaaaaaca aatgaggtta
    46561 tgaatatgaa acgccacatt tacggagaaa ctgaaggtaa acagatttac caatacactt
    46621 ttaacaacga gcatggcatg tcggtttcta ttatcaacta tggtggtatt gtaacccatt
    46681 tgctggttcc cgacaaaaaa gggaatctgg ctgacatagc gcttggttac gacagtttgt
    46741 cctcttacat cgaaaacaat aatcctcatt ttggcggaat tatcggaaga tatgccaacc
    46801 gtattgctat gggcaaattt atgttagacg gcaatgaata tcaattagct actaacgatg
    46861 gtcttaatca tattcacggc ggcatcaaag ggttagataa agttgtgtgg gatgttaaag
    46921 atttcattta ccccgacagc gctggattaa tccttactta tctaagccaa aatggcgagg
    46981 agggttaccc cggaaattta aatattagcg ttacctatac tctaacaaac aataacgagc
    47041 taaagattta ttacgacgca tttaccgata agccaacccc tatcaatctc acacaccact
    47101 catatttcaa cctcgcaggt caaaatagcg gaaccattta cgatcattgg ttgcagatta
    47161 attccaaaag atttgttccg gttaatgcaa acctgatccc aaccggagag ctggccgctg
    47221 tggaaggaac gccaatggat ttccaaattg ccaaaccgat aggaaaagat attgacaaag
    47281 ttgaaggagg cttcgaccat acttttgttt tcgataattc cgataatcta ttaaaaatgg
    47341 tggcacaggt aaatgaacct aaaagcggca ggataatgag agtctttact gatcagccag
    47401 ggatgcagtt ctattcagga aattttcttg acggcagcta tataggtaaa ggaggggtag
    47461 cctaccataa acatttcggt ttttgccttg aaacacaaca tttccccgac tcgccaaatc
    47521 agccaggttt cccttcgaca atccttaggc cggatgagca ttttcgctct tcaacaatat
    47581 attggtttgg aactgaaaaa taaactatta tctctgcgcc actctgggaa ataacgacat
    47641 aaggtgtaac tttaaattgc cccgcaattt ttatcatcac tcactacccg taaaggacat
    47701 taaaatgaat tgatttcgct tacccctgat taaataataa atggaagcta atgttccata
    47761 acatcttttt tataattaaa aaacattaaa ctcctgcttt taaaatgact gttttaatag
    47821 caaagagaat tatcacggct tattttaagc ccatatagtc aggttacaga aatatcattt
    47881 ttgcgccatc attagtttcc gaaatataga attcgggtat gaatccggtt ttatttttat
    47941 acctttcgcc aacctgatca ataaaactgt caacggccgt ttcgttcaaa atagtaattg
    48001 cgcagcctcc aaaaccggcg ccggtcattc tggcgcccaa cacgccatca actttcagag
    48061 cttcttccac cattacatca agctctttgc cggtaacttc ataatcgtag cgcaacgaat
    48121 ggtgtgaagc attcatcaga ctgccaaaaa gctttatgtt gccatttttt aaagcattaa
    48181 ccgcatcgag tacacgttgg ttttcagtta caacatgtgt aactctacgg cgaatggttg
    48241 agtcagtaat taaatgtttg aggctattat actgactgag ggttagttgt cctaaataat
    48301 caacaggatg aacttgtttt ataaaatcaa ttgccttggc acattcggca actctttcgt
    48361 tatatttgga ttgagctaac tgccgtttta ttcctgtatt acaaatgacg attttataac
    48421 cgtcaaaagc gaggggtaca agttcaaaat cgagggtgcg acaatcaaga aaaagggcgt
    48481 gatttttacg gtttatggta acagcaaact gatccataat cccacaattg acgccaacaa
    48541 attctacttc agctttttgc gatagcttaa caatttctaa atcattaatt cctaaattaa
    48601 aataatgatt gagaagagta gctgtggcca cctcgataga agctgacgag gagaggccgg
    48661 ctccgttggg gatattgccg taaaacaaca tatccaggcc ggtaagtttc aaacctcttg
    48721 acagcaattg gttaattacc cctaagggat aatttaccca actcacttta ctcttttccc
    48781 caattttatc aattggaatt tccgcagttt ccgaaaaatt cagcgataaa aaccttaaaa
    48841 caccaccact attactttga gctaatagcc aggtaccgtt tgatatagcg catggtagta
    48901 catatccgcc attgtaatcg gtatgctcgc caataagatt aatacgccct ggcgcaaaga
    48961 cagcctttaa attaccctcg ccggtttgat aacgctcttt aaattcaagt tgtaattcat
    49021 caaaagtcat aattgctttc aatatttatt taaaagcact aaactataac ttttttgcat
    49081 ttaaggattt atttgaattt tcatttacag gtttcattat ttttgccttc aaacaaatta
    49141 atatcaatac agtgaaagta aaattttatc aagcttttgc atttccggtt ttcacccttt
    49201 ttcttgctct gcaactcctg tttaattcac tcaatgcaca gcaacgcata ccacgtttta
    49261 ctgaagaaga aaaaaattgg agtgaatacg acctcaccgg ctggcaattt ggcattaacc
    49321 tcgggatgta tttagctaac aaaaattcgg ctgcttttta caatggcctg cccggcaacg
    49381 aaaacaatat agcctacatt ttaaaaaatt attattggta taacgaaatt aagcaagaac
    49441 ttaatggccg cgacatactt aaattcccta acacccaaaa gcctgaatgg accggcgccg
    49501 gcaatcaatg ggcaagcgag tataacctaa aacctggcga tacggcttgg tgggtttatt
    49561 atccattaaa tctgaagtac gacgctgcta tatcacctgg gttttacgca aaatacaatt
    49621 tcaataacac taccgggatt ttcgttcagt caaactatgt aaagctgaaa acaagcggta
    49681 tctttcaact tgttattgac tctattaccg gcttctccga accggcgctc aggcatggat
    49741 ttatacgagg cgaggaggag cgaataagta ttgatgtggg cataagtaag cattaccgga
    49801 ctggcagggc aacaagcttt tttatcgaaa caggtttttc actaaacagt accgaggtgc
    49861 ttagccataa aattatgata ggaaatcggg agtattcaat tgtaaatgtg tatcttaacc
    49921 aaaactgggt gccaaattca aacgtaacag catataaggt ctatcaaggc gggcttggtt
    49981 acgggctgtt cctcaatggt ggatttaagt ttattttcag tgaaactatc tctattgacc
    50041 cgggaataca gctttatttt aaaaatatca acctaaagcc atacaacgaa ttcgctacca
    50101 actattactt ctatgtgcgt atgatcttta gctttttcgg cgagatatga tcattttgta
    50161 ttaaaccgga tacagaggta atgtgtaacc ttgaattagt ttgacatcgc tttattggcg
    50221 gtgatgccaa ttacgaaacc ttcaccatga aggttagtcc ggttggagct attgcaattg
    50281 acggaggata tggctcatta cccatgatag aaccatataa aatcaactat tgattatcaa
    50341 aacggatagc gatggcctca cctttacaat taataataaa aatttgtcca ttagcacatc
    50401 ggatgcaatc ctttttcccc aaccctgcca ttaacttcat aatcatggat ttcagccttc
    50461 gttacaaaac cgctaccctg caactgattg accttgccgc cgcatcctct ttgagcagag
    50521 gctcgcttcc aaccgccaac aggcggatat ctcaagcgta ccgcctggcg cctacgttta
    50581 ccgtattttc aacagtataa ggagtagagg aaagcggaaa attggtggtg gagtaaaaaa
    50641 tggaggtaaa taacaccaaa ggcttcgcat cctggcatat tctaataatt aacgccataa
    50701 attttcctgg ttactccacc accaccttct ccgtcctaca cctttcgcca tcccacaacc
    50761 ttatcaggta caatcctgac agcaggtgtg tggtttctat tttatgatac cggcttaaag
    50821 gctgtgcttt gtaaagcagc ttgcctgttg ggctgtatag ctcaattttc gctggcgccg
    50881 gcggcatatt ttccggtagt tgcaaccata aatcggtggc ggcaaggttt gaataaatta
    50941 ttattttctt ttctaaaatt tgtgtggaaa aatattaaaa aaatgtgttt aaaaaatttg
    51001 ttttttaaag atcttttaaa ttcttttgtc gccttaaaaa aaatgtaatg aacatataaa
    51061 ttttataaaa atgataacat ttatccctga aattactttt cgggacaaga gaaaaaggct
    51121 tatcgaagac agatcactgc cttcatcaat aattaaagct ttgtaatcaa ccatatattt
    51181 ctgcccaatg gaatcaagtg aaactactat tgatttatct tcctatccta aaggaatgta
    51241 tttcgttact attaaaaatc aaaatgaatc atttattaaa aaaattatta aacactaaaa
    51301 tcatgaaaag aattattcta atgcttatta ttcttattgt tggcacatca agtttttcac
    51361 aatcgtggaa ctgggctcac agcgctggag ggaacagacc tgattatgct aaagacatgg
    51421 ctattgacct cgataataat atatatgttg ggattacttc taggtcagat acttgttatt
    51481 tcaaaacagg ttattatacc ttatgggggg agactgattt ttttatcgtt aaatacaatt
    51541 ctaatggcaa tgagttatgg gtaaagcaat gtggcgggcc atacgaaccc cattttggag
    51601 gatattccgg taaagatgga attatagcat taaaatacga ccaaacaaca aattcaattt
    51661 acggttatgg tggctttgtt ggtacgtgca attttggcga aataactatt ggttccagtt
    51721 ctccgtatga catggatata tttattgcaa aatttaattt ggatgggaat tgcatctggg
    51781 ccaaaagaat tggatgtacc aatgaggatg aaagatggga ccgcgctttt tcaatgtcta
    51841 tagataacta tggatacctg tacgtatgcg cttaccttcc ctttagcgga actttcgata
    51901 atacaccggc cgaattcggt ggaaatctgg ctaaatttga cccggatgga aattgtttat
    51961 gggtaaaaaa gattattccg gttcaaaata taggtttaat tcccccatta ttttttcttt
    52021 acagctatat tattaacgag tctctatatc tcaccggtta caatatcaaa tctgaattta
    52081 ctgtggacac catttcaatg tccattccta attattcagg tcaattgatt gcaagttttg
    52141 atttaaacgg gaatataaag tggcttaagg ctatcggtgg ccctacagga acatcattgt
    52201 taaaggaaat agattctgac gatttggaaa atatttacat ccctggcgct ttttcaggtt
    52261 cctatgcaac attcggaata gatacaattt tttcagatac cggacgcggc atctatgttg
    52321 ccaaatacaa taaagaggga actcttgtat ggttaaaaca tgctgatgta acaaattcag
    52381 caaatgcttc ttctgtaaat aaagatagta aaggattttt atatctttcc ggaggtttca
    52441 gtggtacaat aaattttgac tcctttcaat taaactcaat atccgaaaat gatttatata
    52501 tcatcaaatt cgatcaggaa ggcaattttt tatgtggcga taatacaaat ggcggagcaa
    52561 tctattacct tagaacttac ccaaacgata taataaatat atgcgggtct ttttttggga
    52621 cagcaaactt tggtaataca gttctttttg aaaaaggcgc agtatggggt gatgttttcg
    52681 ttgcccagca cgcaccaatc aatgaaggaa ttaacgaaca taattccaaa atttccaatg
    52741 ctctgatcat ctacgccaac cccaccaccg gcatctgcaa cattaaaata ccggaggagc
    52801 tgcggtacga ggaaaatctt tcactgttag tgtacaataa ttccgggaaa ctcatcctgc
    52861 aacaggaaat caacacacag gaggaaaaga taagccttaa cttagaggct agagctaaag
    52921 gaatttacaa tgccatcctc accaacggaa aacagaaatt ccagggaagg gtggtgtttg
    52981 agtaatttgt tttccaattt caccccgtga atcctaccga tctctccacc aaacttcaaa
    53041 cttaggcggc tggtgaagta aattcatggg gtgaattttc cgacctactc cacacccgct
    53101 ttggctttta aacttacact gatgacctat attccctcac cgaaagtggg ttgttaagga
    53161 ttggctaaac aattgcagat atagtggata tcagaattag tatatcaaaa acatattccg
    53221 ataggcgata ttgagtttac ggttggtcta atattttttc atctttaaaa accagattaa
    53281 ttgccagttg taatgagtaa aatttagcat ttccaaaagt gaacggtgct acgaaattct
    53341 cactgataag atagatttga aaagcgccca gattagcgcc aatagcagcg ctcggcgaaa
    53401 agagcgtact tttggaaaag gtatttgaaa acattaaatg caacttatct tttaaggagt
    53461 aagtatatgc aagtgtaaaa gtattccaat tttggttttt aataaatctg ctttggtaaa
    53521 gcaatccaac atgatggctc ggtttcggtt gatagttaac attgacattc acccatgaag
    53581 gcaatgaggt acgatactct ttcttgtttg tgggaattcc gatctcgtaa aaaattgagt
    53641 cgagcgcttt gctaagggcg ctgctggcaa gcgttttgga atcaaaataa tcctcaagtg
    53701 ggaatccttc atgaacgaat tcctccgtgg tatctaaagt ctcataattt tttaaatttg
    53761 atttccatct gacaaatccc acattcactg cgctaagcgt tgcagataac ttctcattat
    53821 atttatactc agcgccaaca tcgaaagcaa aacctctatt ttcggaatta aaaaatatat
    53881 cgaaaaaatc tgacttattg ggcaaagagg tgttaaccat tacattcgac ttcattttta
    53941 ttgtataatc aacggggtcg gtaaagagta aaaaggaaaa attattagaa gtaatattgg
    54001 ctataccgtt taaatatttt acattcatcc ccattgacaa ttgtgggttt atcatttttg
    54061 aaaatccgag caaatattct cttacatagc taaaattcaa tccattacca ccaagatcaa
    54121 tatctttacc caagaatgca gaatgtccgt tgccataaag gaaaaactga aaggtttctt
    54181 tggtaattgc agtatttaca ttcattttat cggcaatgcg aaagttgaaa tagttatctt
    54241 taattttaat tcctgccgat aaaaactcca aagagagatc aaaattgata ttattattta
    54301 gctttaaatt atttacaaaa taatcctgtt caaaatatat tgaatctcct ttccgaataa
    54361 aaaagtcgtt atagcaaagg gtattctcaa atcccatttt taaggaagac aatataggaa
    54421 cacctacata tagcctatga tcaggaaatg ctgcagggtt aactaacttt ctttgtggta
    54481 tatccggcag aaagaaaagc gacctctcat tttgagcttg cagttgacaa ataatcaata
    54541 caagattaat aaacattaaa acattaagta tcctattttt tttcatgtcc ttatttttca
    54601 agtttcatct ataaattaat acccgttttg gcccctactt ttattttaaa ggcataatca
    54661 tcatatattt ttgcgagatc gccattggtt gtagataatt ccgctttaat aatcagtttt
    54721 tcggcgttaa taattctttc taatcttgaa tcgtacaaaa cgatgtcggt agttttatgg
    54781 gatggttttt ccacttttag tttcggtggc agtcctactt ttcccccttc aataatcctg
    54841 atatcattat cataaatgag cgaatcgagg atattatact ttgaatcagt aaaatagatt
    54901 tgcaaaccgg cgttcaatgg aaatccatta taagcattaa ctctgcaaat cagataatca
    54961 atcgtactca gggttgccgt actcatttca aaatctacgg tatcctggat acttagtttt
    55021 gctatttcga aatagagttt aagctctaag gtagtctgaa acctaagttt actggtatcc
    55081 tgcaggacat tttgtaatgt agtgtcgcca gataagttga tatttgcttt aaaatcgtag
    55141 taaagcttcc gtggcgccat acttaggaac acttctggaa aattggtatt gctaaaatct
    55201 aacctcgtat aaacagattt gccaatctct gccggagttg gggataatat ctcaaacata
    55261 tttggttgcg actggccaaa aaggtaaata tccttataat agggagccgt gaaaggacta
    55321 tatacctgca aagtttgagc ttcaatagta attggcaccc ctacctcgtt atatgtttcc
    55381 acaacaaaca ataaagcgtc atgaccaaaa cctaccgccc caccaagaga attgttgaag
    55441 accgatatct caaccgtatc aactaaatta aaatcataat aaccaatata gccataaaaa
    55501 tccaaatact ccatgttggt catatcgcca ttgatttcaa tttcataggg cgacaaatca
    55561 ggatttgaat caggttgtat gaccaactga ctaataaccc taattctatt ttttccacca
    55621 gacaaaagcg gatctatcga aagtttataa tccttcaaac tctcgttagc cgaaaagtta
    55681 acccaatcct gttggccacc gggattgtca agaacaagat ctatactcaa ctgatcattt
    55741 gtttgaacat taatgatatc ggggcttgtc aacttcatgg atgctttttc tttgttcaga
    55801 tttgtttttc cgaatatgga aaaggtgcca gccttaaaaa aaacactatc aatacgcttg
    55861 ctttcacttg aagaataaaa tgtgaacgaa aactccatag taattgtatc gggattgacc
    55921 gatggaaata tcaacaaagg aaaggtgtgt tcgaaatggc gatcatctaa gaaaaaatag
    55981 ccctcagctc tatctgatga taggttttta ctttggtaaa caagacttat cagtccatta
    56041 tcatccgttt taataaattt ctgattttcc tccgaaaaaa aatccttaat ggcaaattca
    56101 gaataaacca aaggaatagc atactccgga ctccaatcaa tttgtgcaaa tttgttgaaa
    56161 tccaaatcat ccattatttt gctacacgaa aaaagcaaaa tagcaaatgt aaaaagcagc
    56221 aaaaaagatc ttttaaacc
//
LOCUS       DAOOAI010000003        48187 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_913_length_48187_cov_5.201301, whole genome shotgun sequence.
ACCESSION   DAOOAI010000003 DAOOAI010000000
VERSION     DAOOAI010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 48187)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 48187)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48187
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_913_length_48187_cov_5.201301"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3140
                     /locus_tag="PK345_00470"
     CDS             <1..3140
                     /locus_tag="PK345_00470"
                     /inference="COORDINATES: protein motif:HMM:NF016260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HPX33526.1"
                     /translation="LNLETVDCTTKGAVNYLWLNGYNYENINLDGHFKQKQYEGYIAM
                     KDENLDFIFNGLIDLNEAKPVFDFHSDIYLADLYNLKLVSQDSISRLSTNLDINFTGI
                     KLDNIEGFIKVSNTVYNNSENQYQLKNLDITTFTANDGMRSLVLNSDFVDASFTGKFN
                     FSELSLFFNQFLANYSNVLPEKQTENVQNDTPHLIDFSVYIKKSDELSELFLPQLKLS
                     PDATLIGHFHSQDKKLDIEFCADYVDLHPILFENGRIVTSSDDNNFYLDVTAEKVYFG
                     KQQTDSTDRLGIDSLQLITFFRTDSLFYNLSWIDMYKKNLNHGDIVGTLIIDRTDLLK
                     NHIRQFDLLIDGQSWTVIPENEIFIKPKSVEFNQMAFFNNESKLVIDGVISDSESDSL
                     KLYFKDIDISNVDKLFKIPEIDINGILNGNATITSFSERPTFFVNIYLDELYLNDEEL
                     GLLTFDTQWDNFDQSLDVMLNIIKKGNIASTNVLALSGNYFPMRNDRNFDFDVQLHNL
                     GLYLVNPFINDFASINQGGYVSGKLRIDGTYAQPVLSGTLNIMRTQFLLKYLNTLYSL
                     TGIVDVNENVISLNSLSLNDTRGRPASVAGNISHNYFSDFALDISIAHNNFKVLNTTL
                     KDNDLFYGNANSSGIFTMKGPIDDLMMEISATTEKGTQIKIPISTDISVAKNNFIIFK
                     TPETEKAVVEKVYDVNLKGLEIDLTVNVTEDAEIELFLPYEMGTIEAFGKGEMGIGVS
                     KRGDFTIFGDYIISKGEFVFTFEELFSRKFDILKGSKIGWRGDPFDGTIDLQATHKVR
                     TSLNGLQFQMDSTSLFNTKVDVLCVINLQNDLFNPDIRFSIDFLNVPETTKEVIYASL
                     DTTDQSAMSQQILSLLILNSFSYTDYAPGLTSNSFNLLSNQINNLLSRISKDFNIGIN
                     YQPGTALTEDELEVALSTQLFNNRLSIDGNFGVKGKTSSQNTSNVIGDVNLEYKITDD
                     GRFRIKAFNRTNNLPIIENNAPYTQGIGIFYRREFDHFLKFLKSSGKTSKSNYQDLIQ
                     LQREATIEPAPTNKD"
     gene            3394..4410
                     /gene="tsaD"
                     /locus_tag="PK345_00475"
     CDS             3394..4410
                     /gene="tsaD"
                     /locus_tag="PK345_00475"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HPX33527.1"
                     /translation="MALILGIESSCDDTSAAVLFNRTMLSNVTANQQVHQKYGGVVPE
                     LASRAHQQNIIPVIHEALSMAGVTKNDLNAVAFTRGPGLLGSLLVGASFAKAFALALD
                     IPLIEVDHMMAHILAHFIQLPDEKKALPRLPFLCLTVSGGHTQIVLVNDYFDMTIVGQ
                     TIDDAAGETFDKAAKIMGLPYPGGPMIDKLAQQGDPNLFEFPEPQVAGFNYSFSGLKT
                     SFLYFLRDQLKHDPDFIQKNKYHLSASLQAVIIRILLKKLKQASDHFKINEIAIAGGV
                     SANSALRNAIEESKQKWGWNTYMPQFQFTTDNAAMIAITGYFKYINKNFTNQKTTPYA
                     RGGF"
     gene            4528..4878
                     /locus_tag="PK345_00480"
     CDS             4528..4878
                     /locus_tag="PK345_00480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33528.1"
                     /translation="MILAVFSSCSSKLTGTWKIASYEEGVVGEPSVTMTNIGSITFEK
                     NGSGTKAVTFSLMGAAMEDNEPFTWKQAGISVLIEGGESELSKAWVISSKKKGSQTWH
                     STENNKVQTLILIK"
     gene            complement(4997..8536)
                     /gene="nifJ"
                     /locus_tag="PK345_00485"
     CDS             complement(4997..8536)
                     /gene="nifJ"
                     /locus_tag="PK345_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HPX33529.1"
                     /translation="MTKKKKFITCDGNYAAAHVGYMFSEVAAIYPITPSSTMAEYIDE
                     WSSHGRKNIFGETVRVIELQSEGGAAGTLHGVLQAGALGTTYTASQGLMLMLPNMYKI
                     SGELLPGVLHVSARSLAAQALSIFGDHSDVMAARNTGFALLATGSVQEIMDIAGIAHL
                     VAIKSRIPFVHFFDGFRTSHEIQKVEYLENEDLAKLLDWEALKAFRDNALDPGRPVTR
                     GTAQNPDIYFQTREVANRFYDPVPDLVEHYMQEITKLTGREYHPFTYYGSDDAEHIIV
                     AMGSITSTIKETVDYLISKGEKVGMISVHLYRPFSKKYLLKALPKSVKRIAVLDRTKE
                     PGATGEPLYLDFKEIFYDNENAPLIVGGRYGLSSKNTTPAMIISVFENLKLKEPKNQF
                     TLGIVDDVKFTSLPLLPEISIVPKGTFEGKFYGLGSDGTVGANKNSIMIIGDNTPKYC
                     QAYFAYDSKKSGGITISHLRFGDKPIQSSYLVGTPDFVACHVPAYLEKYDMLRGLKKG
                     GTFLLNSTWDAEETKNRLPDHMKKYLAENDIKFYIINATKIAEEIGLGGRTNTIMQSA
                     FFKISEVIPYDLAVDEMKKFIIKSFGRKGEEIVNMNFAAVDKGGNIEKVEVPKEWANI
                     QLVKQKDERNLPDFVKNVMEPIVAQNGDDLPVSAFLGREDGTFPSGTTQYEKRGIAVN
                     VPQWIPENCIQCNLCSYSCPHAVIRPFLLNEEEMAKAPKDLKTLKAIPKTFDGLQFRI
                     QISPLDCTGCGVCVDVCPAKTKALQLKLLETQMHEQDNWDFITKNVTYKDNIVEKDKS
                     VKNSQFARPLFEFSGACAGCGETPYIKLITQLFGDRMMVANATGCSSIYGGSAPSTPY
                     CTNAEGRGPAWANSLFEDNAEYGFGMLLGVNKMRQRLADKMKEALKNGISDEMKNAFN
                     TWLENINDGEGSKKASADVIKVCKNSNTKIAKEILDLQQYLIKRSVWAFGGDGWAYDI
                     GYGGLDHVLASGEDINVLVLDTEVYSNTGGQASKSTPVGAVAKFAAAGKRTRKKDLGA
                     MAMTYGYVYVAQVAMGANQAQYLKAVREAEAYPGPSLIIAYSPCINHGLHAGMGKSME
                     ESNRAVEAGYWQLYRYNPLLELEGKNPFQLDSKEPDWDKFQDFLDGEVRYTSLKLAFP
                     AEAEHLAKEALKNAKWRYNSYKRLAAMDFSPQE"
     gene            complement(8948..9436)
                     /locus_tag="PK345_00490"
     CDS             complement(8948..9436)
                     /locus_tag="PK345_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33530.1"
                     /translation="MDTSSLIMGLVLVSICIVPLYLFNLNRKNKKKKLLQALSNIAAK
                     HNCTISQYDVCGENIIGMDEAKKYVFCFKKQNDEIQEHFVNLAEIKNCKTTHTTRNVK
                     HNDEVQKITEKVELSFIPINGSEPNINWEFFNEEVKSHMTGELQLVEKWTKLISAQLK
                     SK"
     gene            complement(9670..10041)
                     /locus_tag="PK345_00495"
     CDS             complement(9670..10041)
                     /locus_tag="PK345_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33531.1"
                     /translation="MKKILRTAALFLLATIYCFAMNVVTQSIVSFNYNSDVHSGEERY
                     LERVYSIFFYHTPHNETASNTVQNFVAHEFKNLFHFIWATQETIENKFEKSFTLYTWN
                     SENIQIRYRKTNLIFPFHNFW"
     gene            complement(10098..11447)
                     /locus_tag="PK345_00500"
     CDS             complement(10098..11447)
                     /locus_tag="PK345_00500"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPX33532.1"
                     /translation="MKNKKLYFIIVPIILLLVILAILKSKGVLGKDQSLKVNTEQVTR
                     RTIIQTVSANGRIQPAKEVKITPYISGEVVELWVREGDQVKSGDLLAKIDPEIYLSNY
                     ERMEAGLQSQRANLSNTKARLLQTKAQFTSSKLSFDRNEKLWQEKVISDSDYETALAQ
                     YEVAKAEVLASEEAVKAAEFQVASAEASLREAKENLTKTAIFAPADGTISRLTVEKGE
                     RVAGASQFSAGTEMMRIANLDLMEALVKVSENDIVRVNLGDTSLIEIDAYLNRKFKGI
                     VTEIATSAETTTATTDQVINFEVKISILKDSYLDLLDSEKPWASPFRPGMSTTVDIQT
                     KTEYNTLTVPIQAVTTRSDSTGKSIEARIDHDMNKEKDKNSIKINTEITEYVFVFQND
                     SAKMIEVNTGIQDNTHIQILNGLEEEQEVISGPYRAVSRTLKNGDKVKKVPKEKLFE"
     gene            complement(11483..12874)
                     /locus_tag="PK345_00505"
     CDS             complement(11483..12874)
                     /locus_tag="PK345_00505"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPX33533.1"
                     /translation="MNNVCLLKRFLIPILVFFLFLPDKGLTQNRIWTLEDCIYYAFEN
                     NLQIKQSALNVKNFEISKLEAKLSLIPNLNSDASFSYNWGRVLDRTNYTYTDKETNQA
                     NFGIHANMRLFGGLQLQNQIKKASLDFLTSQYDYDKIKDDITLLLTQAYLNILYNKEL
                     LKVATNQVSITRDQIRLTEKLMEAGKLALGNLLEIQAQGAREEITEINYENSLALAYL
                     DLMQLLDIKADTDFEIEVPVLDDELLFEITPINQVYDVALERMPSVKSAEAAVESAYR
                     GIAIAKGARSPVLNLQSGWNTNYSNQYRKFEIDPYTGQYYQTDVIPFGDQFVDNQSRF
                     IGLSLSIPIFNAYQATSNISRARVAASNAEYNYELAKNTLRKSIEQAFYDAQAAYKSY
                     QATQKSLSSSVEAYRHTEQRFTVGMATSVDYNLAKTQLAAAESDLLRAKYDYIFKTKI
                     LDFYMGRPITLNQ"
     gene            13112..15484
                     /locus_tag="PK345_00510"
     CDS             13112..15484
                     /locus_tag="PK345_00510"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPX33534.1"
                     /translation="MIKKGILFFLLLSITLFETQAFAHFSEVNSKRSHLSNGNLSDTL
                     DALKYEIEITDLDFVAKTITAKTTVTLKSKIDILNVVKLELLDLIVDEVFVNGNPVSN
                     FQQQNPFLSIPLSSPINSGEIIEVKVHYHGSPFHENWGGFHWSGQYAFNLGVGFTSDP
                     HNLGKSWFPCIDDFHDRATYELFVTVTNPKVAVCGGTLLDVIENGNNTKTYHWKIEDE
                     IPTYLASVAVGEYINKPSSFVSVTGEEIPIGIYVRPSDTAKVQGSFQTLVPMLEAFEQ
                     YLGPYRWERVGYVSTAIGAMEHSTNIAIPHATITGGLSYESLFAHELSHQWFGDLVTC
                     ASAEDMWLNEGWAVFCESLYREALYGWQAYHDNMFALQRTVLHSAHVSDNGYRALYGI
                     PTQYTYGTTVYNKGGIVVHTLRNYMGDDLFFPAVRAYLDEYAFNHADTWQLRDILSNH
                     SGVELTDFFETWVFTPGFPEFSIDSFNVISSGNNWDVTVFVKQKLKGPCQFANSNRIE
                     VTFMDQQWQKETVLMEFSGETGQQTFTININPVIAMLDFYDKVADATTDKYLTINAST
                     NLNLTDVFVKLRVDEMPLSDSGFIRITHRWVPPDPLQTPLEGLTLSDYRHWRIDGILP
                     QGTQLTGSFMYNRFNNLDGNLMANPADSLVILYRPNTNSEWVGVDFTKIGAPNTGYLE
                     TPNIQPGDYTLAVWSSQVNVNDNMSKHKNDIMIYPNPAQEKVYFEFKSLIPTQIKIFN
                     ANGQIVGNIKNLNASSLMEWDTSNQNKGVFIIQFIDKQNKLMRTDKILIE"
     gene            15484..16164
                     /locus_tag="PK345_00515"
     CDS             15484..16164
                     /locus_tag="PK345_00515"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HPX33535.1"
                     /translation="MHVIVTDVIKRFEESADKQRALQQKAYMRNQFEFYGISSPERAL
                     IYRSIFALHNPVNQSIIEEVIPELWSLQQRECQYFAIELAKRHIRQMDSAFVNTIEYM
                     ICQKSWWDTVDLIATNLVGKLFQKFPELILHYITKWATSENLWLQRTTLLFQIKYKDK
                     TDAALFFKLIESFAGHPDFFIRKAIGWALREYSRTDPQLVAEFVKNHQLSSLSTKEAV
                     KLLKKRKL"
     gene            complement(16245..16949)
                     /locus_tag="PK345_00520"
     CDS             complement(16245..16949)
                     /locus_tag="PK345_00520"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPX33536.1"
                     /translation="MTRSAELNQLIFNHLSAFITEHKLKRFDEVLKNRTRYITVVIED
                     VYQPHNASAVLRTCDCFGIQDVHIIENQNTYTINPDVALGSAKWLSLFKYSGENNNTP
                     TAIKLLKEKGYRIIATSPHSDNIVLEELSLEKGPVAVMFGTEMRGLTQTSFQLADEFI
                     KIPMFGFTESFNISVSAALVLYHLTDKLRKSTIDWELTENDMIDIRLQWAREVVKRSD
                     LIERDFLSKNPLGKEE"
     gene            17135..17824
                     /locus_tag="PK345_00525"
     CDS             17135..17824
                     /locus_tag="PK345_00525"
                     /inference="COORDINATES: protein motif:HMM:NF024572.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX33537.1"
                     /translation="MAKKTTKTEKNIQAVEEALSKSERFIVENQKPITYILVALIALV
                     LIYFGFHRFYIQPKEQRAQKDMFMAEKYFGQDSLNLALNGDGINPGFLDVARRYSFTK
                     SAGLANYYAGAIYMKQGEFDEAIKHFKKFKSKDELLKPMALGAIGDAYVELDNNSKAI
                     SYYLKAAKSSKNEFTTPVFLLKAGWTYELMGDYKNAIGIYKKIRKEWPTSNEARDLEK
                     NISRAEGLLKK"
     gene            17943..18911
                     /gene="fmt"
                     /locus_tag="PK345_00530"
     CDS             17943..18911
                     /gene="fmt"
                     /locus_tag="PK345_00530"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009123547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HPX33538.1"
                     /translation="MKRDLRIIFMGTPQFAVPSLESLIDNGFNVVGVITAPDKPAGRG
                     REIKFSEVKQFAVKAKLPLLQPVNLKDNKFIKEFAALKPNLGIVVAFRMLPEEIWSFP
                     EFGTFNLHASLLPQYRGAAPINHAIINGEKVTGLTTFFIKHEIDTGKIIFRNPIKIGE
                     QETYGELHDRMMLTGAELVLKTAFAIQDNSYDLISQSDLITTDEIIKSAPKILKADCR
                     INWNSKSVTIYNLIRGLNPVPAAFTVLSGPNNEEIQLKIFKASYKMLTHDHKPGEIFI
                     TSEKQIATFTSDGTLTIEELQMAGKKRLQVKDFLNGFPITPKWKMH"
     gene            19069..19353
                     /locus_tag="PK345_00535"
     CDS             19069..19353
                     /locus_tag="PK345_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HPX33539.1"
                     /translation="MSSTMNKAELIDAMAAESGLTKADSKKALDAFVKVTQNALKSGD
                     KITLVGFGTFSVSTRAARIGRNPQTGKELKIAAKKVVKFKAGSDLAGAVA"
     gene            complement(19369..19998)
                     /locus_tag="PK345_00540"
     CDS             complement(19369..19998)
                     /locus_tag="PK345_00540"
                     /inference="COORDINATES: protein motif:HMM:TIGR00567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HPX33540.1"
                     /translation="MQDPFMILKPKFYLNSNVVEVARLLIGKVLVTRFENKLTSGIIV
                     ETEAYAGTDDRASHAWNGKITPRNRVMFEQGGVAYVYLCYGIHSLFNVVTNVAGTPHA
                     VLIRGITPLDGVDIMLARRGKTYLKPSDGIGPGKVSMLLGIHYSHSGLPLTGSESSSL
                     QIWIEDRGALTNGEIRTTTRIGVDYAGKDALLPYRFVMDIKKAPINGAF"
     gene            complement(19973..20719)
                     /locus_tag="PK345_00545"
     CDS             complement(19973..20719)
                     /locus_tag="PK345_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPX33541.1"
                     /translation="MKIVVVLPAYNAAKTIENTYNEIPFDIVDEVVFVDDKSSDNSVE
                     IAHKLGIKHILIHPENKGYGANQKSCYQKALELNADIIIMLHPDYQYTPKLIHSMAYL
                     IANGVYPVVFGSRILGTGARKGGMPVYKYIANRILTLFQNLILNQKLSEYHTGYRAFS
                     AEVIRKVNFLANSDDFVFDNQIISQIFMAGFEIAEITCPTKYFEEASSINFHRSVKYG
                     LGVLKTSFLYRFHRWGIINSSLYARSVYDS"
     gene            complement(20783..22684)
                     /locus_tag="PK345_00550"
     CDS             complement(20783..22684)
                     /locus_tag="PK345_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009122724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HPX33542.1"
                     /translation="MDIQQILIKYWGYSGFRPMQEDIIRSVLAGQDTLALMPTGGGKS
                     ICFQVPALAKEGICIVISPLIALMKDQVDRLKALGINAFAIYSGMHQTEIELALNNAT
                     LGNAKFLYISPERLATPNFREVLRYMKVNLIAVDEAHCISQWGYDFRPPYLKIAEIRH
                     IIPDAPVLALTASATPVVVDDIQEKLGFSTPNLIRQSFERKNLAYIVVKEENKMGKLV
                     EMFKKVHGSAIVYAGTRRRTYEFARELNKNGIPADHYHAGMENEQREKKQKAWMSGHP
                     RVIVATNAFGMGIDKPDVRLVVHVDLPGSIEAYFQEAGRAGRDGKKAWSVILYQDSDL
                     LDANSQFEFSFPDMEMIKSVYHALGNYFSLAVGSGKNTGFDFDISDFCNQFNLNPIST
                     FNSLKFLEKEGYLLLSEGLHFPPRIFVKLQGEKLYKFRVENADLDIFLKIILRSYTGL
                     FTQFTKFNINELARRTSLSVEQTFAKLEKLNRLGAIAFVPPKSKPQIIFTEERLDAKD
                     LYISKENYSMRKKMALERLNSLNVYLTSNSRCRSISLLNYFGESKIKRCGQCDVCLER
                     NKVDLNEQEFNAILDQIKPLLKTGAFEINDLIAQTERVKKEKVLKVIEWLLDNNKIAY
                     TLDKKLYWL"
     gene            23279..25264
                     /locus_tag="PK345_00555"
     CDS             23279..25264
                     /locus_tag="PK345_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPX33543.1"
                     /translation="MLGIFFLILLGLSFGVFFFRKKSGYLYSLLLVMGMVALTTHGAV
                     QALNGNSQSFVHGFNNIFRSATDILVLDGLSAFFILVINITLLVGLLYANGYLKPYHS
                     RMGNLRFAVHYFSYIWLYFSMLLVVMLRNGVLFLIAWEMMAISSFFLVIFDADNRNIL
                     KTGINYLVQMHVGMLFILVGFLLVEKSTGSISFDSLPTYFANSSNWPLFLLFFIGFAI
                     KAGFMPLHTWLPEAHPAAPSHVSGVMSGVMIKMGIYGIFRVVLATQSDLLIIGVLLLA
                     VSLISGLLGVIYAILQHDLKRLLAYHSIENIGIIGIGLGLGIIGKATNNQMLMLLGFS
                     GALLHTLNHSLFKSLLFFNAGSVYLSAKTRDIEKCGGLMKTMPYTALLFLLGSIAICG
                     LPPFNGFISEYLIYFGMINTMHGAGFSQSILLLFSIIGLTLIGGLAVFCFTKAFGVIF
                     LGSPRSKQAENAIEVTHSMIAPQIISAILIVAIGVVPALFVKPIFGIFSHDLKIIIPE
                     YHSFIANLSGISAASGVFIIMAVGILLLRYIHLRRKPIAAGPTWGCGYTLESSRLQYT
                     ASSYANSLSQLAKPVIRRKIITEPISENDIFPPSRAFSTHSEDVFREQLTNKPVNKFL
                     DWMKKIAIMQTGQIQHYILYAFLFMLLILILTYLNFI"
     gene            25268..26176
                     /locus_tag="PK345_00560"
     CDS             25268..26176
                     /locus_tag="PK345_00560"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HPX33544.1"
                     /translation="MLGIILIIIASLLVPGVIIRTKSIASGRKGPGLFQPLKDLRVLL
                     RKGSVFSNTTGLIFRIAPSLSLASIVCALTLVPFGNQGALITFGADFVFFAYILSFGK
                     FFAIIGALDCGSSFEGMGANREALYSMMVEPAFFILLGTFAMFTGYTSFTDIFYGFHL
                     SDNNYILIFSVISFYLLIQIVMIENSRMPIDDPKTHLELTMIHEVMILDYSGFDKALL
                     HIGTFLKYGIYGALITAILINTKWSIYIQIFAFFAVVVCLGVIIGLNESFRARNKMGK
                     NPQFVLTLSGIALVAFVIVLLLMGKF"
     gene            26189..26818
                     /locus_tag="PK345_00565"
     CDS             26189..26818
                     /locus_tag="PK345_00565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33545.1"
                     /translation="MLNFLIVLFAINLIYVSVADRFRIYSRLIAVQGILLMGIALIEL
                     QEVSTLNLILILAETLIFKAIVVPYLLFSIIHRTGIYKVHNRALPGFFSLILTIIALV
                     LSIVIANILENPHIDTAYLTIALFTMFVGVLLIIGHKLIFSHMIGFLIIENAVFMLSM
                     AVGKEMTMLINIGILLDVFVSVLVISILINRIGTEFNKFEVQDLTSLKH"
     gene            26822..28249
                     /locus_tag="PK345_00570"
     CDS             26822..28249
                     /locus_tag="PK345_00570"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HPX33546.1"
                     /translation="MILFYFISAIILSVATLLVNKRRTAQIMISLFAAIQIAFAYYAY
                     LHLNQTDLSYFTYDPLGVIFLSVLSIIFPATIYHGFRYFTNGTTKRFYYYHSALIGVI
                     AVITGAYLANEATVVWILVEATTLLASVLIYHEKTKMALEATWKYIFLCSVGIAFAYM
                     GILFIGFGIEQYGFTNLSFEAIAKVASQANPLYLKIAFIFIFIGYSTKMELFPMHTVG
                     IDANSVAPSPIGALFSTAIVNMGFVIIFRIYVALSGSEIFHWMNHVLIIGGLSSIVVA
                     AGYMLKATHHKRMFAYSTLENAGLAAIALGIGGAGYYAAILLLILHSFVKSSMFFQIG
                     QLNRVFNSLDINFSGKYVKLHPVGAVVLMVGMIFLLAIPPSGLFIPKYLIFKALIFSN
                     SWFVLIAVALMICVVVYALSVRVMHICFSDARKSMHDVLPEKINPIETVTQFLFLLVV
                     VVFCFYRPPLVDELIRQSISLLTQM"
     gene            28253..29770
                     /locus_tag="PK345_00575"
     CDS             28253..29770
                     /locus_tag="PK345_00575"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HPX33547.1"
                     /translation="MEGKKYIEVSNSQAVAIKDIPLLDYDDFYDYVVDELKIDTHHCL
                     NYYCVDFGSRLWFVCCIACDELNIIRICSHELPKGEKSLRSITPECFQLHIFEREIHE
                     NFRIHFKGHPWLKPVRSSFKNDGTKLHVDAYPFLAFESEELHEVGVGPIHAGIIEPGH
                     FRFICHGEKVLHLEISLGYQHRGIEQLFVKQNGLQRTILTESVAGDTAVGSSIAHAQL
                     IESLADLPIPESLMIERCIGLELERFAIHIGDTSALCTDVAYQLGQVVNEALRTTIIN
                     TTQFWCGNRFGKGLVRPGGSNYPLNETVVDEIRNILKDSGNRYIDMADRIYTLPTVLS
                     RFDGIGELTPEQAILIGAVGMAARSTSLGRDIRKSHPFQYFNQYQYEPVVLTTGDVLA
                     RGMLRYEESKKSVSMVNEFCTIWEGMAKEDTPRPVYDYGLKPLSFAISVVEAWRGELC
                     HSAITDEQGNLAHYKIKDPSLHNWMALALAVRNQEISDFPVCNKSFNLSYCGNDI"
     gene            29760..30530
                     /locus_tag="PK345_00580"
     CDS             29760..30530
                     /locus_tag="PK345_00580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone oxidoreductase"
                     /protein_id="HPX33548.1"
                     /translation="MISRKKLRIKEANILRSHGWQYIPDLKGVELSPLFRGRPVISDT
                     ITPEEIEHVKNICPVDAFVDDPFGLDMGKCIFCKECHFACPQKIMFTNDYKIAASNRE
                     DLVIRVGQTKMDFDHSAVRPVIRKLFKQSLKLRQISAAGDNSSEMELNASGNVNFDMG
                     RYGIEFLASPRHTDGIVITGPISENMADALQICYDAVPEPKIIILAGSDAIGGGMFVD
                     SPAINRTFLDKYHIDLFVPGNPMHPLTFINGVLSLIGK"
     gene            30841..33156
                     /locus_tag="PK345_00585"
     CDS             30841..33156
                     /locus_tag="PK345_00585"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HPX33549.1"
                     /translation="MDNLFRKDALSYHAEGRPGKLEVIPSKPYNTKRDLSLAYTPGVA
                     DPCLKIKDNPDDIYKYTMKGNLVAVISNGTAVLGLGDIGTLASKPVMEGKGFLFKIFA
                     DIDVFDIEIDLKDPQKFVDTVVAIAPTFGGINLEDIKAPECFEIEEKIQAAVDIPVMH
                     DDQHGTAIITAAGLLNAVKLTGKKIEDLKIVVNGAGASAISCTRLYIKLGVRPENIVM
                     IDSKGVLRKDRTDLNYVKQQNTTDRDIYTLEEAIEGADMFLGLSAAGALKKEYLKKMA
                     PNPIVFALANPVPEIMYDEAMAVRDDLIMATGRSDYPNQINNVLGFPFIFRGALDVRA
                     TCINDEMKLAAAYALAELATEPVPDEVNVAYHATNFAFGKDYIIPKPFDPRMITRVSS
                     AVAKAAMDSGVARKPITDWEAYNEFLLKKMGTTNPITRQIRHRALANPKRVVFADAEN
                     YKMLKAAEFVLNQKSAYPVLLGDEKIIKALIQQHELDIPNPEIIDPRSPAEDDRRKQF
                     AKLLFEKQNRKGITNYTALDKMLHRNYFAPMLVETGFADAMVSGLTNNYPDAIKPAIE
                     VIGIKQQANLVSGMYIINTKSGPLFFADCTVNKEPTSEQLVEITLQTAFAVTQFNVIP
                     RVAMVTYSNFGSNKGRIPDKVAEAVRILHREYPKLIVDGEIQADVALNSEMLNENFPF
                     SKLQGNPANTLIFPYLTAGNIAYKLLREIGKFDVIGPILTGMNKSVHVLHFGNSVQEI
                     INMVMVAALDAQCVQNRELNKCKDDFKEIFR"
     gene            complement(33365..34099)
                     /locus_tag="PK345_00590"
     CDS             complement(33365..34099)
                     /locus_tag="PK345_00590"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33550.1"
                     /translation="MKKLIFSLIASLLIIPFAFSQSFELYYENEIFPFGGTIYVVDEA
                     SASLMQAHISIKNISNAAKLVKVKKEEVQVVPGSMNTFCWVVCWANTIFVSPAGITID
                     PGVTNDEFSGDYMPFNNVGVTIIRYTFFDDDNPNDSTHFFVNFNAGPVGIDDLTKNTK
                     NSFSLAYPNPASNQVSFDYTLSDQTINAYIRVHNLLGTVVKETRLDYFSGKLILDVCD
                     LNEGFYFYSIVVNNEVIDTKRLVISR"
     gene            34318..35397
                     /locus_tag="PK345_00595"
     CDS             34318..35397
                     /locus_tag="PK345_00595"
                     /inference="COORDINATES: protein motif:HMM:NF024491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine/phosphatidylglycerophosphate/
                     cardiolipin synthase family protein"
                     /protein_id="HPX33551.1"
                     /translation="MAKSDHTNYQLFDDPIRFYNVILDDIASAKKYVFIQMFKFSHQA
                     MGMKFRDSLTAVALRGVEVKLLIDSWGGSSFPYNFFDELVSAGGEVRFFEKIKINIDI
                     FTRSHRRNHRKLIIIDDIISYIGSSNISDYNLIWRESMLRIYGSIAVKFKKVFNQDFS
                     AYKKYFRYKESFTRIIRHGDMEILRDSPSVTRQKIKKRYEEVIKNALKEVIIVTPYFL
                     PGFNLRKVLMDAANRGVEVKVIIPKRSDIRMVDILHGRYLEILNNSNVKFLYFTKHNL
                     HAKLLLVDNEKFSIGSPNFDYRSFRYMHEIVLFGTTPSIIKLIREFIIDTQQYCEPFD
                     YEAWQRRPAIQKFFEWVLLPFRHLL"
     gene            35364..36839
                     /locus_tag="PK345_00600"
     CDS             35364..36839
                     /locus_tag="PK345_00600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HPX33552.1"
                     /translation="MGALTLSASPLTILLYNFAGSFKITDMATQKEIKTKSKSRKKEI
                     LPLDNEGEPTKIFVLDTSVILYNHNSINSFKEHNVAIPITVLEELDQFKKGNDTINFE
                     AREFIRIMDKLSANASLQNWISLKKKGLGKFKVIMNNSAKSMDANEVFGESKCDHRIL
                     NAALALQHEHPERKVIMVTKDVNLRIKAKAVGLTAEDFLTGRIENIDSLYSGISRLEN
                     VDKRGIDRLYEEGLCEVEDLNIENPKPNQYFILQTDGASALGYYNPRNQLIERVDKHT
                     VSKINPRNAEQVFALHAVTNPNIRLVTLQGIAGTGKTLLALAGAWSQRRNYKQLFLAR
                     PIVPLSNKDIGFLPGDAKLKIEPYMQPLYDNLRFIQNQFGEHDKEFQNITAAMQNEKL
                     VIQPLAYIRGRSISNVFFIVDEAQNLTPHEVKTIITRAGEGTKIIFTGDIYQIDTPYL
                     DSQSNGLSYLIDRVKHHNLYAHVRLEKGERSDLANLANEML"
     gene            complement(36880..37896)
                     /locus_tag="PK345_00605"
     CDS             complement(36880..37896)
                     /locus_tag="PK345_00605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018669367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPX33553.1"
                     /translation="MKLKNIRLLSILLLLLLMLSCNNNKAGKKTKKSSDPTPTNVSIQ
                     VPEFNADSAYFFIEKQISFGPRVPESEAHSNCAEWIKENLQRFCSHVSLQEFKARAYN
                     NQILNGKNIIAVFNPNKQPRYLLCAHWDSRPYADHDPDPANHQKPIDGANDGASGTGV
                     LMEVARQLSLNSPSAGVDIVLFDIEDYGPPQDRQAYESTDFWGLGSQFWSRNPHTTHY
                     SANFGILLDMVGAPDAQFPLEGFSMYYAPDIGKKVWEIAGRLGYGDRFVFDNGGYITD
                     DHYFINRIARIPTINIIHLDQSSSNGSFFEHWHTINDNMDHIDTNTLKMVGDVVLNVV
                     FKGL"
     gene            complement(37927..38523)
                     /locus_tag="PK345_00610"
     CDS             complement(37927..38523)
                     /locus_tag="PK345_00610"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPX33554.1"
                     /translation="MEKPAITKVPIQSLLTRRWSPRAFSLKPVDTHQLQRIFEAARWA
                     PSANNDQPWSFIVGIDNDETYQKIFSSLVEFNQLWVKFAPILVVSIGQKISKKSGKQN
                     RWYSYDVGQAVAHLSIQAMHEGLFVHQIGGFDAEVLINDFEIPDDQVPISVFTIGYIG
                     DYNELHPNLKELELKERTRKNQNDFLFSGKFGMKLSLG"
     gene            38829..39824
                     /locus_tag="PK345_00615"
     CDS             38829..39824
                     /locus_tag="PK345_00615"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HPX33555.1"
                     /translation="MRLAIIGSTGMVGRKMLQVLEERNFVIDSLILAASDKSVGNKII
                     FQSKPFEVVSMQEAFAQKPHIALFSAGSTVSQAWAKRFVEQGTFVIDNSSAWRLHNDV
                     PLIVPEVNIDALQPNKPLIANPNCSTIQLVVALAPLHRAFKIKRVVISTYQSVTGSGV
                     HGSNQLRCERANLPTIPFYPHQIDLNLIPQGGSFNSDGYTTEEQKLIDEVRKILSDTS
                     LQITATVVRVPVYGGHSESVNIEFYTKFELDEVKRLLKMMPGIVIQDEPEKSIYPMPF
                     YAQEKDDVFVGRIRRDFSVENGLNIWVVSDNLRKGAATNAVQIAEYIVRKGWIKG"
     gene            complement(39851..44122)
                     /locus_tag="PK345_00620"
     CDS             complement(39851..44122)
                     /locus_tag="PK345_00620"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPX33556.1"
                     /translation="MQWQFNKSFLFLILLPIIGLRLYPQSITINEFMSANSYFITDED
                     GDYSDWIELYNYGTETINLFGFGLSDDDSNPFRWKFPAITILPDQYILVWASGKDKTI
                     PGMPLHTNFSIKVSDEPLILTNPYGTTICEVSPITLPSDISYGRQPDGNDKWFFFIEP
                     TPNAPNQTQAYPGILDHPQFSHQGGFYTDDFWLTISHIDPEVIIYYTLDGSEPNPANM
                     NVFTFIYKDKYPFNPRDPFGKFLTSTFQTYQFNDSLHIENICARPDSITHISTTFDNN
                     LCYFPENPVFKGTLVRAMAFKEGWIKSPTITHTYFVTSFGRERYSLPIFSLSVGKNDL
                     FDYNIGIYTAGQDFDEWRMANPESPIDWQTKANFHRIGIESEKRSNLEFFDVNDNFSA
                     FSHEIGFRINGYESRRRPLKSIRLYSRSEYGQPNFNYKFFKNLKDDQFKTLILRNSGQ
                     DYFQTMLRDAAIQQMVKDLIPDIQEVMPAILFINGEYWGIHNLRERFDKDYLKRVYDI
                     DAGNIDFIVNYNEVREGDTVHYNETKEYIKQHGLAEKEHYEYIQTRIDVDNFRDYQII
                     NIFADNHDWPGNNLEYWRMRTSQYNPNAPTGQDGRWRWFVKDLDFGFGLFGLEEALQH
                     NMLQFATAAGGLEYPNPDWATFLLRKFLENEEFKISFINRFADLLNTTFLPSVMQNKI
                     LEAENKIDIEVSEHITRWKNISSYQLWLEQMEVMYHFAEQRPAYQNQHIIDFFDLSGQ
                     YSLEVDVSNHWFGKVRINSIEITDDTPGINESAYPWSGNYYLGIPIEVEAIALPGYVF
                     THWSGDTTTTDPILNLLPAKNLYLKAHFKKNNAPTLVNYWFFDTSLPNNTPLEVIEAS
                     YSLLNDATLTFHSSIDGYPFYAGHPNWRKASMERRNEPTNINYRPEGNNNIPYNLSNT
                     KGIQVRQPFIGDGGENIMNFQLPTTGIRDVVFRFAAKDEGAADNLIIEYTIDTEPENW
                     TSEGLSNPTPLLSGEYQLFEFDFKNIEATNDNPDFGIRIRFDGDNMSADDGNRVTFNN
                     ISLDGIVITPDNSPPVFIAQIPLQQCIEGNEEAILINLDKYVMDPEGDAIYYSFINMH
                     PQVAQTDLNGSILTILPIQRGACNIIILVSDCFTEPVASSFIITVYPKAFELRKNKIS
                     FNKWDENQPENSFPEHMIFLQSDKNDPDLYDELLFAYFIPHNVYAPEDEQNIGFPYKN
                     NYRTRINGLDNKGIAIVNTNAERDLGGFLIALDTREVNFGFINWTAELLEQNQKKYAI
                     RLFYRFNVTEPFRELIIKNKVLEYNPEMHGSLMNFLDIPLPDYILNKQYVQLLWKFYY
                     FSGNYGKSHRIRLDDINIADITYVPKLNFAYIHLYSYNNNIYISFPERTYAAIDIYDV
                     AGRLIKTRQLAGANKYSIDMYPFKGIFIARIYLNNDVYVKKFFVN"
     gene            complement(44174..46075)
                     /locus_tag="PK345_00625"
     CDS             complement(44174..46075)
                     /locus_tag="PK345_00625"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33557.1"
                     /translation="MKHFVNKLTFNAFIAVFIALFGFILIHSGQSVNCTTSISNPSSN
                     LVFFWLFDTSLPNDTPLEAIDPIYNLNPNAILLYNSALSGYPFSPDHPNWRKASMERR
                     NAPTPLNYRPEGNNGISYDVSNMRAIQVKQPFVGDGGENTLYFHLPTNGFKDIVFQFA
                     AKDEGAADKLSIDYSVNSGEPVWTTTGLANPTPDLSDNYQLYVVNFNQINEASNNQHF
                     KIRIRFIGENMSVDEGKRVTFNNFSIDASALSGNNLPPVIINNIELQELIEQGDGMEF
                     NLNNIFSDPDNDPLVFSVVSERPTMVQATLTGSTTSITPLKRGDAIITITANDGFNPT
                     VQTSFRVLVYPRAFPLKDGELSFTYWNYNEPEYSYPQYMMFLQSEQNDPKINDALLFP
                     YFIPHDDYHPDDLANLGFPYKNSRRTRINGLDDDGIAFINTGRGRDLGGLLIAVDLSE
                     VESAKLDWLGGTIIRNNRVYAIRLFYRTNLFTPFADLMHNGQEIEYVTAQQGDVKRFS
                     DISLPDNIMNQKYVQFLWKYYLVEGDTSSRAQLRLDDIVFKNVTGINESQKAKFDIYT
                     KDGEIILTSPTIVNGDIMVYDVMGRLIKTNKIRNSTTSVVDLGMIKGVFVVRLITSEN
                     NFSTKILLR"
     gene            46313..48166
                     /locus_tag="PK345_00630"
     CDS             46313..48166
                     /locus_tag="PK345_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HPX33558.1"
                     /translation="MADNIKKPVRELENVVIRFSGDSGDGMQLTGTLFSDTSALFGNY
                     LATFPDYPAEIRAPQGTVGGVSGFQVHFGHGEVNTPGDYADVLVAMNPAALKANTKWV
                     KSGGIIIVDIDSFTEKNFQKAGYNNDPLTDPKMKDYNVIQAPITSLTQESLKDLNLDN
                     KSVLRSKNMFALGMVYWLFDRPMDFTNKFFENKFKKNPVVLEANKTVLKAGYQYAESS
                     EALQSSYSVAPAKIEKGIYRNINGNVATAWGLLAAAEKSKLTLFLGSYPITPATDILQ
                     ELSTRKDLGVKTFQAEDEIAGICSAIGASFAGHLAVTTTSGPGLSLKTEATGLAVITE
                     LPLVIVNVQRGGPSTGLPTKTEQADLLQAIYGRHGESPLVVLAASTPSNCFHYAFVAA
                     KIALEHMTPVILLTDGFLANGSEPWKIPEMKDLPDIKPPLIKEGAEDYKPYKRLNDNM
                     VRGWAVPGTKGFEHRLGGLEKMAVTGAVSYVPENHQVMVNEREKKVEAVVNDIPDLKI
                     LGEESGELLLIGWGGTYGHLYTAFRELRKEGFKLSYIHFNYIKPFPKNTAQILNSFKK
                     RLVCELNLGQMANYLRMKFEGLSFLQYNKVQGLPFTVIELKEQIKKILEGK"
BASE COUNT        14833 a         9067 c         9323 g        14964 t
ORIGIN      
        1 ggttaaattt ggaaactgtt gactgtacta caaaaggcgc tgttaattac ctatggctca
       61 atggctacaa ttatgaaaac ataaacttgg atggccattt caaacaaaaa cagtacgaag
      121 gttacattgc catgaaagat gaaaatcttg atttcatttt caacggattg attgatctta
      181 acgaagcaaa gcctgttttt gattttcatt ccgacatata cttggctgat ctgtacaacc
      241 taaaattagt cagtcaggat tctatttcac gtctttctac aaatctagat atcaatttca
      301 ccggcataaa actcgataat atcgaaggct ttatcaaggt aagcaacact gtttataata
      361 acagcgaaaa tcaatatcag ctcaaaaacc ttgatataac tacctttaca gcaaatgatg
      421 gaatgcgttc cttagttctt aattctgatt ttgttgatgc atcatttacc ggaaaattta
      481 atttctcgga gctgtcacta ttttttaatc aatttcttgc aaactattca aatgttttac
      541 ccgaaaaaca aactgaaaac gtccaaaatg acactccgca tcttattgat tttagtgtgt
      601 atataaaaaa atcggatgag ctttcggaat tatttttacc tcaacttaaa ctctctccgg
      661 atgctacact cattggccat tttcattcgc aagacaaaaa gttggatatt gaattttgtg
      721 ccgactatgt tgatttacat ccaatattat tcgaaaacgg tagaatcgtt acctcatcgg
      781 acgataacaa cttctatctt gatgtaacag ccgaaaaggt ttattttggt aagcaacaga
      841 cagatagtac agatagactt gggattgaca gcctccaact gataactttt ttcagaaccg
      901 attctttgtt ttacaattta tcatggattg atatgtacaa gaaaaaccta aatcatgggg
      961 acattgtcgg aacattaata attgatcgga ctgatttgct aaaaaaccac atacggcagt
     1021 tcgacctgtt aatagatggg cagagttgga cggttattcc tgaaaacgag atttttataa
     1081 agccaaagtc agttgaattc aatcaaatgg cattcttcaa taatgaatca aaactagtga
     1141 ttgatggagt gatatcggat agtgaaagcg atagtttaaa gctttatttc aaagatattg
     1201 atatcagtaa tgttgacaag cttttcaaaa ttcctgaaat tgacataaat ggaattttaa
     1261 acgggaatgc caccataaca agctttagtg aaaggcctac tttttttgtt aatatttatc
     1321 ttgatgaatt atatctgaat gatgaagagc ttggacttct aacttttgac acacaatggg
     1381 ataattttga tcaatcgttg gatgttatgc taaatataat aaagaaaggg aatattgctt
     1441 ctaccaatgt cctggcgtta tcaggcaatt actttccaat gcgtaacgat agaaatttcg
     1501 attttgacgt acagcttcac aatttgggat tatatcttgt aaatccgttt attaatgact
     1561 ttgcaagtat taatcaaggt gggtatgtaa gtggtaaact caggattgat ggtacttacg
     1621 ctcagcctgt gctttcagga acgcttaata tcatgcgtac tcaattccta ttgaaatatt
     1681 taaatacact ctattcactt accggaattg tagatgtgaa cgaaaatgta attagcctaa
     1741 actcactgtc gctgaatgat acaagaggaa gaccagcgtc ggttgccgga aatatcagcc
     1801 acaactactt ctccgatttt gctttagata taagtatagc acacaataac tttaaagtac
     1861 tcaacactac attaaaagat aatgacctct tttatggaaa tgcaaattca tcgggcatct
     1921 tcacaatgaa agggcctatt gacgacctga tgatggaaat aagcgctaca acagaaaaag
     1981 gtactcaaat aaaaattccc atctctaccg atattagcgt tgcgaaaaat aacttcataa
     2041 tatttaaaac ccccgaaaca gaaaaggcag ttgtagaaaa ggtttacgat gtcaatctta
     2101 aaggtttgga aattgaccta acagttaatg taaccgagga tgctgaaatt gaattgtttt
     2161 tgccttatga aatgggtact atcgaagctt ttggaaaggg tgaaatggga attggagtta
     2221 gtaaaagagg agattttact attttcggag attatattat ttcaaaagga gaattcgtat
     2281 ttaccttcga agaactcttc agtcgtaaat tcgatatttt aaaaggtagt aaaattggtt
     2341 ggcggggaga tcctttcgac ggtactattg acctacaggc tactcacaaa gtaagaacat
     2401 cacttaatgg gctgcagttt caaatggatt caacttctct ttttaataca aaggtagatg
     2461 tgttgtgcgt cattaatttg caaaatgacc tttttaatcc tgatatcaga ttttcaattg
     2521 attttttaaa tgtgccggaa acaaccaaag aggtaatcta tgcttcactt gatactaccg
     2581 atcaatcggc aatgagtcaa cagattcttt cattattgat tttaaatagt ttcagttata
     2641 ctgattatgc ccctgggctt acttccaaca gttttaattt gctctcaaat caaataaata
     2701 acttgctttc gaggatcagc aaagatttca atataggtat caattatcaa cctggcacag
     2761 cgttaacaga agatgaattg gaagtagccc taagtactca gctattcaac aataggttat
     2821 ctattgacgg taattttgga gttaaaggaa aaacatcaag tcaaaataca agtaatgtaa
     2881 ttggcgatgt taatcttgaa tataaaatta ctgacgatgg ccgttttagg ataaaggcct
     2941 ttaatcgtac aaacaacctt cccatcatag aaaataatgc tccatacact cagggaatag
     3001 gcatttttta ccggcgtgaa tttgatcatt ttcttaagtt cctcaaatca tccggaaaaa
     3061 cttctaaatc aaattatcaa gatctgattc aattacaacg tgaggcaact atcgagcctg
     3121 caccaacaaa taaagattga tgctgataac gattttgatt tatctgaaaa cttgtaatat
     3181 ggagctaaaa gactttttac cattacgttg ttcattaaaa gtgtattggt tgtcaaatgc
     3241 cattataagg agttagtgaa ttattaaaaa gtcaagaata gacgattagg agatgaatat
     3301 tcatcatttt gagtcgaaca ataaattaca aattgtgctg gacaaagatt taatttgggt
     3361 taaactctat ttttgtttaa aaaatcaaaa actatggcct taattcttgg gattgaatct
     3421 tcatgcgacg acacttcggc ggccgttttg ttcaaccgga cgatgctctc taacgttact
     3481 gccaatcaac aggttcatca aaaatatggt ggagtagtcc ctgaattggc ttcgcgtgct
     3541 caccagcaaa acatcattcc agtaatacat gaagccctct ctatggcagg agttacaaaa
     3601 aacgatctta atgccgtagc cttcactcgt ggtccagggc tgctgggttc tcttcttgtg
     3661 ggcgcctctt ttgcaaaagc tttcgccctg gcattggata tcccgctgat agaggttgat
     3721 cacatgatgg cgcacatact tgcccatttt attcaactgc cggacgagaa aaaggcgtta
     3781 ccccgattgc cttttttatg cctgacggta tcaggtggac atacacaaat agtgttagtg
     3841 aatgattatt ttgatatgac aattgtaggt caaactattg atgacgctgc tggcgagacc
     3901 ttcgataaag ctgcaaaaat aatgggtctt ccataccccg gcgggccaat gattgataaa
     3961 ttagctcagc agggagatcc taatttgttt gaatttcctg aacctcaggt tgctggtttc
     4021 aactatagct ttagcggatt aaaaacatca tttctatatt tcttgcgaga tcagttaaaa
     4081 cacgacccag attttattca aaaaaacaaa taccatctca gcgcatcatt gcaagcagta
     4141 attatcagaa ttttattgaa aaagttaaag caggcatccg accattttaa aataaatgaa
     4201 attgctattg caggtggcgt atcagcaaat agtgcattac gaaacgcaat tgaagaatca
     4261 aaacaaaaat ggggttggaa tacatatatg ccacaatttc agttcaccac agataatgct
     4321 gcaatgatcg ctattaccgg gtattttaaa tacatcaaca aaaattttac caaccagaaa
     4381 acaactccct atgcccgtgg agggttttaa ttaaattata atagtaattt ctcgacactt
     4441 gttttaattc atatttttgc acaaaatatt ttttaattta aatcaaaaaa aactatgaaa
     4501 aaggtattaa ggtttacaac aattattgtg attcttgcag tattttcttc ttgttcaagc
     4561 aagttaacag gtacatggaa aatagcaagt tacgaagaag gagtagtcgg agagccatcc
     4621 gttaccatga ccaatattgg atccattact tttgagaaaa acggtagtgg tacaaaagcg
     4681 gttactttct cacttatggg tgcagcaatg gaagataatg agccatttac ctggaaacaa
     4741 gcaggcataa gtgttcttat agaaggtggt gaatccgaac tgtccaaagc atgggttatt
     4801 tcatcgaaga agaagggctc gcaaacctgg cattcaaccg aaaataataa agtgcaaact
     4861 ttaattttga taaaataaaa ggttgaataa tagttttatt attcttttgt ttgcaaaaca
     4921 aacccgtata aatttagtta gtataaaaaa agaccttgca aattcgcaag gtcttttttt
     4981 atctatcaaa taaatgttat tcctgaggtg agaagtccat ggcggcaagt cgtttataac
     5041 tattatatct ccattttgca ttcttcaatg cctcttttgc cagatgctct gcttcggctg
     5101 ggaatgctag tttcaaggaa gtataacgca cctcgccatc aaggaaatcc tggaatttat
     5161 cccagtctgg ttctttggaa tcaagctgga acggattttt tccttcaagt tccaacaatg
     5221 gattataccg ataaagttgc cagtatcctg cttcaactgc cctatttgat tcctccattg
     5281 acttacccat tccggcatgt aatccatgat taatacatgg tgagtaggca ataatcagcg
     5341 aagggccagg ataagcctct gcttcacgta cggctttaag gtattgagct tgattagctc
     5401 ccatggctac ctgtgccacg taaacataac cataggtcat cgccattgcg ccaagatcct
     5461 tcttacgcgt tctttttcct gctgcagcaa atttggctac ggcccctacg ggagtggatt
     5521 tcgaagcttg tcctccggta ttcgagtaaa cctcggtatc taatacaaga acgttaatat
     5581 cttcaccaga ggctaataca tgatcgaggc cgccatatcc aatatcataa gcccaaccat
     5641 cgccaccaaa agcccaaaca gatcttttaa tcagatactg ttgaaggtcg agtatctctt
     5701 tagctatctt agtgttagaa tttttacaaa cctttatcac atctgccgaa gctttctttg
     5761 atccttcgcc atcgttaata ttttcgagcc acgtattaaa cgcatttttc atttcatcgg
     5821 aaataccatt tttcaaggct tctttcattt tgtctgccaa acgttgacgc attttattaa
     5881 cacctagcaa cataccgaag ccatattcgg cattatcttc aaacaatgag tttgcccatg
     5941 ctgggcctct gccttcagca tttgtacaat aaggagttga aggagccgag ccgccgtaaa
     6001 tggaagagca tccggttgca ttggctacca tcatcctatc accaaaaagc tgagtgatca
     6061 attttatata tggcgtttcg ccacaaccgg cacaagcgcc tgagaattca aataatggcc
     6121 tggcaaactg gctgttttta accgatttgt ctttctctac aatgttatct ttatatgtta
     6181 catttttagt gataaagtcc caattatcct gttcgtgcat ctgtgtctca agcaacttga
     6241 gttgtagcgc ttttgttttt gcaggacaaa catcaacaca cacaccacat ccggtacaat
     6301 ccagcgggct gatctgtatc ctgaattgta gtccatcaaa agtttttgga atggctttta
     6361 atgtcttcaa gtctttcgga gctttagcca tctcctcttc attcagcaaa aacgggcgaa
     6421 taactgcatg ggggcaagaa taagaacaca gattgcactg aatacaattc tcgggtatcc
     6481 actgaggcac attcactgca ataccacgtt tttcatattg agttgtgcct gaagggaaag
     6541 taccgtcttc gcgacctaaa aatgcactca caggcaagtc atcgccattt tgagcaacga
     6601 taggttccat gacattctta acaaaatcgg gtagatttct ttcatctttt tgtttaacta
     6661 actgtatatt agcccactcc ttaggaacct cgactttctc aatattgcca cctttgtcaa
     6721 ctgctgcaaa gttcatatta acaatctctt ctccttttcg gccgaagctt tttatgatga
     6781 attttttcat ctcgtcaact gcaagatcat aaggaattac ctccgaaatc ttaaagaatg
     6841 ccgactgcat aatggtattt gtgcggccac ctaacccgat ctcttcagca atttttgtgg
     6901 cattgataat gtaaaatttg atatcatttt cggccagata tttcttcata tgatcgggaa
     6961 ggcgattttt ggtctcctcg gcatcccatg tgctgtttaa gaggaaagtt ccaccttttt
     7021 tcaaacccct tagcatatca tacttttcaa gataagcagg aacatggcag gccacaaagt
     7081 caggtgttcc aacgagatag gatgactgaa tgggtttatc gccgaagcga aggtgagaaa
     7141 tagtgatacc gccggatttt ttagaatcgt atgcaaaata ggcctgacaa tatttgggtg
     7201 tgttatctcc gataatcata atggagtttt tgttagctcc aacggtacca tcggagccga
     7261 gaccataaaa cttaccttca aaagtgcctt ttggtacgat cgagatttca ggcaacagag
     7321 gcagagacgt aaactttaca tcatccacaa taccaagtgt aaattgattc ttaggctctt
     7381 tcaatttcag attttcaaaa actgaaatta tcattgccgg tgtagtattt ttggagctaa
     7441 ggccataacg tccacccacg atcaatgggg cattctcatt atcataaaat atctctttga
     7501 aatcgagata gagcggctcg cccgttgctc ccggctcctt ggtacgatca agaacagcaa
     7561 tacgtttaac cgattttggt agagccttta gtaagtattt tttggaaaaa ggcctgtaaa
     7621 gatgaacact tatcattccc actttctcac ctttcgagat aagataatca acagtttctt
     7681 ttattgtgct ggtaattgag cccatagcta ctattatgtg ctcggcatca tcagatccgt
     7741 aataggtaaa tgggtgatac tcacgacctg tcagctttgt tatttcctgc atataatgtt
     7801 caaccaaatc gggaactggg tcataaaatc tatttgccac ctcacgggtc tggaaataga
     7861 tatctggatt ttgtgctgtt ccgcgtgtta caggacgtcc tggatcaaga gcattatctc
     7921 taaatgcttt caaggcctcc caatccaata atttagctag atcttcattt tctaaatatt
     7981 caactttctg gatttcatgc gaggtacgga agccatcgaa aaagtgaaca aatgggattc
     8041 gtgattttat agcaaccagg tgagcaatac ctgcaatatc cattatttcc tgaacgctac
     8101 cggttgctaa taaagcaaaa cctgtattgc gcgcagccat tacatcggaa tgatccccaa
     8161 agatcgaaag agcctgtgct gcaaggctac gagcgctaac atgtaaaact cccggaagca
     8221 attcacctga gattttgtac atattgggaa gcatcaacat caaaccttgg gatgcggtgt
     8281 aggtagtgcc taaagcgcca gcttgtaaaa caccgtgaag cgtgcctgct gcgccaccct
     8341 ccgactgtag ttcaataacg cgaacagttt caccaaatat atttttacga ccatgagacg
     8401 accactcatc aatatattca gccatagtgg atgatggtgt aataggataa atggcggcga
     8461 cttcactgaa catgtaaccg acatgagcgg cggcataatt gccgtcgcat gtaataaatt
     8521 tctttttctt agtcatattg ttgttaatta ttaaacataa aaatttcaaa atcgaaaacc
     8581 ggcacgaaat taatcatttt taacatacat taaagctgta agaataaccc cgaagccgtt
     8641 tttgacaatt taaaatgact tcaaattaaa aattaagagt aaactaaata attgaaaaaa
     8701 atattcctgc taaaatatta atactacttt ggttttaaga taaaatgaca atcctgaaac
     8761 caaatattta ttgagtgaat atcagtgaaa ttattgactt tacaattaat tacaaattag
     8821 tatcaatctt aataagtcaa aatagttccc acaatggtat cgtcagaata aaaaaggagg
     8881 cagccagaac cttacctcct tatattattt taaagtctgt tttttagttt aatcaaacac
     8941 ttgattttta tttactcttg agttgggcag agataagttt tgtccacttt tcaaccaatt
     9001 gtagctctcc tgtcatatgt gatttaacct cttcgttgaa aaattcccaa tttatattcg
     9061 gttcagaacc attgattgga ataaaactaa gctcaacttt ttcagtaatt ttctgaactt
     9121 catcgttatg tttgacattt ctggttgtat gggttgtttt acagtttttt atctcggcaa
     9181 ggttaacaaa atgctcctgt atttcatcgt tttgtttttt aaagcaaaaa acatattttt
     9241 ttgcttcgtc cataccaata atgttttcgc cacaaacatc gtattgacta atggtgcagt
     9301 tgtgttttgc ggcaatgttg ctaagggcct gcagcaattt tttcttttta ttttttcggt
     9361 tcaggttaaa aagataaagt ggtacaatac atatactaac cagaaccagc cccattatca
     9421 aactacttgt atccatataa attcaatttt aaataattaa taattaaata gttgaagttc
     9481 ttcgaggcaa tatgttatgt tttacaattg caggaattgg cctcttttag aaaccagtca
     9541 atagcgcaaa acaattgatg ttcatttaag atattttgcg gttgatgcag gtatctattt
     9601 ataaattgtt cagaagaact tcggaattaa aatcgggtaa agcttgaaaa atcaagcgtc
     9661 ggaaatggat taccagaagt tgtgaaatgg gaaaataaga tttgtctttc ggtaacggat
     9721 ctgaatattt tcagagttcc atgtatagag ggtaaaagat ttttcgaatt tattttcaat
     9781 ggtttcctgt gttgcccaga tgaaatgaaa gagattttta aattcatgag caacaaaatt
     9841 ctgaacggta tttgaagcgg tttcattatg gggggtatgg tagaaaaaga tagagtaaac
     9901 ccgctctaaa tagcgctctt ctcctgaatg tacgtctgaa ttataattaa agctgacaat
     9961 ggattgtgta accacgttca tcgcaaaaca atagatcgta gccaatagaa aaagagcagc
    10021 ggttcttagt atctttttca ttttctccat tattgaatcg ctacaaaact aaacaaaaac
    10081 agttattgtt ccacaactta ttcgaataat ttttctttgg gtactttttt taccttatcg
    10141 ccatttttga gggttcgcga tactgcccgg taaggccctg atataacttc ctgttcctct
    10201 tccaaaccat tcaatatctg gatatgtgtg ttatcctgga taccggtatt cacttcgatc
    10261 atcttggccg aatcgttctg aaagacaaag acatattcgg ttatttcggt gtttatttta
    10321 attgaatttt tatccttctc tttgttcata tcatggtcta tccgtgcttc tattgattta
    10381 ccggtcgaat cacttcgggt ggtaacagcc tgtatgggta cggtaagtgt attatattct
    10441 gttttggttt gaatatcaac agtggttgac atacctggcc taaaaggtga tgcccatggc
    10501 ttttcggaat caagcaggtc gagataggaa tctttaagaa tactgatttt tacttcaaaa
    10561 ttgataacct gatcggtagt tgccgtagtt gtttcggcag aagtggctat ttcagtaact
    10621 atgcctttga attttctatt cagataagca tctatctcta tcagtgaagt gtcgcctaag
    10681 tttactcgta caatatcgtt ttcgcttact ttaacaagag cttccatgag gtcgaggttt
    10741 gcaatacgca tcatttccgt acctgccgaa aactgcgagg cgcctgcaac ccgttcacct
    10801 ttttcaacag ttaaacgcga tattgtgcca tcagcaggtg caaatattgc tgttttagtc
    10861 aagttttctt ttgcttcgcg caaagaggct tcagcgctgg ctacttgaaa ttcagcagcc
    10921 tttacagcct cctccgaagc cagtacctcg gccttcgcca cttcgtattg agctaaagca
    10981 gtttcataat cggaatcaga gataaccttc tcttgccaga gtttttcgtt acgatcaaat
    11041 gagagtttag agctggtaaa ttgtgccttt gtctggagaa gccgcgcttt ggtattcgac
    11101 aagtttgccc gttgcgattg aagtccggct tccatccgtt cataattgga aagatatatc
    11161 tccggatcaa ttttagctaa caaatcacca ctcttcacct gatcgccttc gcggacccat
    11221 aactcaacaa cttcacccga aatgtaggga gtgatcttta cctccttagc aggctgaata
    11281 cggccattgg ctgatacagt ttgaataata gtacgacggg ttacttgttc agtattaacc
    11341 tttaaggact ggtcttttcc taacactcct ttgcttttta aaatggcaag aattaccaat
    11401 aatagaatta tcggtacaat gataaaatag agttttttgt ttttcataat tttatatggt
    11461 tcaactgata acagatcgga tattattgat tgagcgtaat aggcctgccc atatagaaat
    11521 caaggatctt ggttttaaag atatagtcat attttgctct tagtaaatcg gactcggcgg
    11581 cagccaactg tgtctttgct aaattataat ctacagaggt agccatacca acagtaaagc
    11641 gctgttcggt atgccggtat gcctctacag atgaggataa cgacttttgt gtagcctgat
    11701 agcttttata tgcagcttga gcgtcataga aagcttgctc gatacttttg cgtaatgtat
    11761 tcttagccag ttcgtaatta tactcggcat tggaagcagc aacccgtgca cgcgaaatgt
    11821 tagatgttgc ctgataagca ttaaatatcg gaatgctcaa gcttaggcca ataaaacgac
    11881 tttggttgtc aacaaactga tcgccaaatg gaatgacgtc agtttgataa tattgccctg
    11941 tatatggatc tatttcgaat tttcgatatt ggttggaata atttgtgttc catccgcttt
    12001 gcaaatttaa aaccgggctt cgcgctccct tagctatagc aatcccacgg taagcgctct
    12061 caacggccgc ttcagcgctt ttaactgatg gcatacgttc aagagccaca tcatacacct
    12121 gatttatagg cgtaatttca aacaaaagtt catcatccaa cacaggcact tcaatctcaa
    12181 aatcagtatc agctttaata tctaataatt gcattaaatc aaggtatgca agagcaaggg
    12241 agttttcgta atttatctcc gtaatttctt ctcttgcgcc ctgagcctga atttcgagca
    12301 ggttacccag agccagcttg ccggcttcca taagtttttc agtaagtctg atctggtcgc
    12361 gggttatgga aacctgattt gtagccactt ttagcagttc tttattatag agaatattaa
    12421 gatatgcctg cgtaagcaat aaagttatat catctttgat tttgtcgtaa tcatattggc
    12481 ttgttaaaaa gtcaagcgat gcttttttga tttgattttg aagttgcagc ccgccaaaaa
    12541 gccgcatgtt ggcgtgaatg ccgaagttag cctggttggt ctctttatcg gtataggtat
    12601 aattagtgcg atctaatacc cttccccaat tataactgaa tgaggcgtcg ctgttcagat
    12661 tgggaatcag gcttagttta gcctccaact tgctaatttc aaaattttta acattaaggg
    12721 cgctctgctt gatctgaaga ttattttcga aagcgtaata aatgcaatct tcaagcgtcc
    12781 atatcctgtt ttgtgttaat cccttgtcag gcaaaaaaag gaaaaaaaca agaatcggaa
    12841 ttaaaaacct tttaagtagg caaacattat tcatgttaaa tgatgttgag taaattgaca
    12901 actcgattcg caacaaaaga aattcttttt tttaatacaa acgaatttca ttaaacttag
    12961 actcaaataa atccggattt attcttttaa ctatctataa aatacaataa ttaagaaaaa
    13021 agtcattatt atctttgaag tcaaaattta gtaacgaagt taaagtgatt tttatacttt
    13081 tacccatcga aaaaattaaa tatttgctta aatgataaag aaaggtattt tgtttttttt
    13141 attgctttca attacacttt ttgaaaccca agcctttgca cacttttctg aagtaaactc
    13201 aaaaagatca catttgagta atggtaacct gagcgacacg cttgatgctc taaagtacga
    13261 aattgaaatt acagatcttg attttgttgc aaaaacaata accgcaaaaa ctactgtaac
    13321 tctgaaatcc aaaattgaca tccttaatgt tgtaaaattg gaattattgg atcttatagt
    13381 ggatgaggtt tttgtaaatg ggaatccggt tagcaacttt cagcaacaaa atccgttttt
    13441 aagtatcccg ttatcatcgc ccataaattc aggagagata attgaggtta aagttcatta
    13501 tcacggttct ccttttcacg aaaattgggg cgggtttcat tggagtggtc aatatgcttt
    13561 taaccttggt gttgggttca catccgatcc gcataacctt ggcaagtcgt ggtttccttg
    13621 tatagatgat tttcacgaca gagctactta tgagcttttt gttaccgtta ctaatccaaa
    13681 agtagccgtt tgcggaggta ctctacttga tgttatagaa aatgggaaca acactaaaac
    13741 ttaccattgg aaaattgagg atgagatacc tacttacctt gcatcagttg ccgttggcga
    13801 atatatcaac aagccctcgt cgtttgtttc ggttaccggt gaggaaatcc ctattggtat
    13861 ttacgtaagg ccaagtgata cagctaaagt tcagggtagt tttcaaacgc ttgttcctat
    13921 gttagaagca tttgaacaat atttaggtcc ataccgttgg gagcgggttg ggtatgtcag
    13981 tacagctatt ggcgctatgg agcattcaac taatattgcc atcccacatg ccacaattac
    14041 cggtggcctc tcttacgaaa gtctttttgc ccatgagctc tcacatcaat ggtttggcga
    14101 tttagttact tgtgccagcg ccgaagatat gtggctcaac gaaggctggg cggtattttg
    14161 cgaatcacta tatcgcgaag cgctatatgg atggcaagct taccatgata atatgtttgc
    14221 attacaacga acggtccttc atagcgcaca tgtttccgac aacggttatc gtgccttgta
    14281 cggaatccct acccaatata catacggaac gacagtatat aataaaggcg gtatcgttgt
    14341 acataccctt agaaattaca tgggtgatga tttgttcttt cctgccgtga gagcttacct
    14401 tgacgaatac gcctttaatc atgccgacac atggcagcta agagatattt taagcaacca
    14461 ttcaggagtt gagttgaccg actttttcga aacatgggtt tttacgccag gctttcccga
    14521 gttctcaatt gactccttta acgtgatatc gtccggaaat aactgggatg taaccgtttt
    14581 tgtaaaacaa aagcttaaag gaccttgcca attcgcaaat tcaaaccgta ttgaggtaac
    14641 attcatggat caacaatggc aaaaagagac tgttctgatg gaattttccg gcgaaacggg
    14701 tcaacaaacc tttaccataa atataaatcc tgttatcgcc atgttggatt tttatgataa
    14761 agtagcagat gcgacaacag ataaatattt aaccatcaac gcatcaacaa atctaaacct
    14821 aaccgatgtt ttcgtaaaac taagggttga cgaaatgccg ctctctgatt caggcttcat
    14881 tagaattaca catcgttggg tgccacccga tccgttgcag actcccttag aaggacttac
    14941 gctctcggat tacaggcact ggcggattga tggaatatta ccgcaaggaa cacaactgac
    15001 cggtagcttt atgtataaca gattcaataa ccttgatggc aaccttatgg ctaatcctgc
    15061 cgactcgtta gtaatacttt accgacctaa tacaaattct gaatgggtag gagttgattt
    15121 tacaaaaatt ggagcgccaa acaccggtta cctcgaaacc ccaaacattc aaccaggcga
    15181 ttatacttta gcagtatgga gctcgcaggt aaatgtaaat gataatatgt cgaaacataa
    15241 aaacgacatc atgatctatc caaaccctgc acaagaaaaa gtatattttg aattcaaatc
    15301 attgatcccc acgcaaataa aaatatttaa tgctaatgga caaatagtcg gaaatataaa
    15361 aaacctaaat gcatcatcat taatggaatg ggatacttca aatcaaaata agggagtttt
    15421 tataatccag tttattgaca aacaaaataa gttaatgaga accgataaaa tattgattga
    15481 ataatgcatg tcatcgttac agatgttata aaacggtttg aagagtcggc tgataagcaa
    15541 agggcgctgc aacaaaaagc ttatatgcgg aatcagtttg agttttatgg aattagctca
    15601 cctgaacgag cattgatcta ccggagtatc tttgctttgc ataatcccgt aaatcaatca
    15661 ataattgagg aggttattcc cgaattgtgg tcgttacaac agagggaatg tcagtatttt
    15721 gctattgagc tggccaaacg ccatatcagg cagatggatt cggcctttgt aaacacaatc
    15781 gaatatatga tttgccaaaa atcatggtgg gatacagtgg atttgatcgc gactaatctt
    15841 gtaggtaaat tatttcaaaa atttccggag ctaatactgc attatattac caaatgggca
    15901 acctcggaaa acctttggct acaacgcaca actttacttt ttcaaattaa gtataaagat
    15961 aaaaccgatg cggctttgtt ttttaagctc attgaaagct ttgccggaca tcctgatttt
    16021 tttattcgaa aagccattgg atgggcgctg cgcgaatatt caagaaccga tcctcagttg
    16081 gtagctgaat ttgtcaaaaa tcatcaattg tcatccctta gcacgaaaga ggctgttaaa
    16141 ctattgaaaa aaaggaaact ttaagaaatt agagatatta gaaaatgagt gattttagct
    16201 tgttaaatcc tcatcagtaa aaaatgtagt tcaaatcagg agcattattc ctcttttcct
    16261 aaaggatttt tgcttaggaa atccctttcg atcagatcac tacgttttac tacctctctt
    16321 gcccattgaa gccgaatgtc aatcatatca ttttcggtta attcccaatc aatagttgac
    16381 tttcggagtt tatcagtaag atgatataaa accaaagctg ctgacacaga gatattaaaa
    16441 ctctctgtaa aaccaaacat agggattttt ataaactcat cggctaattg gaaggatgtt
    16501 tgcgtaagtc ctctcatctc ggttccaaac atcacagcaa caggcccttt ttcaagcgat
    16561 aactcttcaa gaacaatatt atcgctatga ggagatgtgg caataatcct gtaacccttt
    16621 tcttttaaca acttgatggc ggtaggagta ttattgtttt cgcctgagta tttgaaaaga
    16681 ctcaaccatt ttgcagagcc caaagcaaca tcaggattga tagtgtatgt attctgattt
    16741 tcgatgatat gaacgtcttg aattccaaaa caatcgcatg tgcgtaatac agcgctggca
    16801 ttatgaggtt gataaacatc ttcaataaca acagtaatat aacgtgtacg gttttttaaa
    16861 acctcatcaa agcgcttaag cttatgctca gttataaatg ctgagagatg attaaaaatc
    16921 aattgattta attcagccga tcttgtcatt ttaaataagg tgtatggata ttattaacgt
    16981 aaaaaccata atgactacat aattcaacga ctttattttt tcaaacagcc ctcagaaatt
    17041 cattcggata aaaaagaatt gattaaaaaa agccatatac tttccgcttt tgaaaaaaaa
    17101 tatacttttg caccttcaaa ttcgagaaaa aattatggct aaaaaaacca caaaaacaga
    17161 aaaaaacatt caagcagtag aagaggctct gagtaaatca gaacggttta ttgtagaaaa
    17221 tcaaaagccg attacctata tccttgttgc gttaatagct ttagtgttga tttactttgg
    17281 attccaccgt ttttacattc agccaaaaga gcaaagagct caaaaggaca tgtttatggc
    17341 cgaaaaatat tttggacagg attcacttaa tcttgctctt aacggcgacg gcatcaatcc
    17401 cgggtttctt gatgtggcac gcaggtattc atttacaaaa tcggcagggt tggccaacta
    17461 ctatgccggt gccatttata tgaaacaggg cgagtttgat gaagcaataa agcattttaa
    17521 aaagttcaaa tcaaaggatg agcttctcaa gccaatggct ttgggagcta taggcgacgc
    17581 ttatgtagag ttggataaca atagtaaagc aatatcatat tatttaaaag ctgcaaagtc
    17641 gagcaaaaac gaatttacta cgccggtttt tttgcttaaa gccggatgga catacgagct
    17701 tatgggcgac tataaaaatg ctattggtat ttacaagaag attagaaagg aatggcctac
    17761 aagtaatgaa gctcgtgacc ttgagaaaaa tatttcacgt gccgaagggc tcttgaaaaa
    17821 ataatagaag attctaattc aaaataaaaa cctgcccgat tttgagcagg ttttttcata
    17881 aaaacttgaa gcattagcat ttgtagtttt agagaattaa aacaataata aatatttaag
    17941 ccatgaaaag agatcttagg ataatcttca tgggcacgcc acagtttgca gtaccctctc
    18001 ttgaatcgtt aatagataat ggatttaatg tagttggtgt gataaccgcc cccgacaagc
    18061 ctgccggacg tggaagagag atcaagtttt ctgaagtaaa acaatttgcg gtaaaagcta
    18121 aactgccatt actccaaccg gtaaatttaa aagacaataa gtttatcaaa gagtttgcag
    18181 cgcttaaacc aaatttagga atagtagtgg cgttcaggat gttgcccgag gaaatatggt
    18241 cttttcccga attcggaacc tttaatcttc atgcctcgtt attaccccag tatcgtggag
    18301 ctgctcctat aaatcatgca attataaatg gagaaaaagt taccggactt actaccttct
    18361 ttattaaaca cgaaatagat accggaaaaa taattttcag aaatccgata aaaattggag
    18421 agcaagagac ttacggggaa ttacacgata gaatgatgtt aaccggcgct gagcttgtat
    18481 taaaaacagc attcgccatt caggataatt cttatgatct tatctctcag tctgatttga
    18541 ttacaacaga cgaaataatt aagagcgctc caaaaatttt aaaagccgat tgcaggataa
    18601 actggaactc aaaatctgta acaatctata acttgatacg tggtttaaat cctgttccag
    18661 cggcatttac cgtattgtcc ggacctaata atgaagagat tcaacttaaa atttttaaag
    18721 caagctataa aatgcttacc catgatcata agccaggtga aatattcatt acaagtgaaa
    18781 aacagattgc gactttcaca tcagatggta cattaacgat agaggagtta caaatggctg
    18841 gaaaaaagcg tttacaagtg aaagattttt tgaatggctt tccaataacc cctaaatgga
    18901 aaatgcatta aaaattaaag gcaaataagg cgcgattaac aatttttaac gtaaaatatc
    18961 gttaaaaata cctttttaag taatttttaa tcgttttttt ttgtaaaaag cttgtaggta
    19021 ttcatttatg aactatattt gcaggaatgt ttttttttac taatcaaaat ttcatcaact
    19081 atgaacaaag ctgaacttat tgatgcaatg gctgccgaat ctggtttaac caaggcagat
    19141 tctaaaaaag cattagatgc ttttgtaaag gttacccaaa atgctcttaa atcaggagac
    19201 aaaattactt tagtaggttt tggtacgttt agcgtttcaa cgcgtgcagc aagaattggc
    19261 agaaacccac aaactggtaa agagctgaaa attgctgcta aaaaagttgt taaattcaag
    19321 gctggaagtg atttagcagg cgctgtagcc taatctaaaa caaaagaatt aaaaagctcc
    19381 gtttatcggg gcttttttta tgtccatcac aaacctgtat ggcaacaggg catctttgcc
    19441 ggcatagtca acacctatcc ttgttgttgt tctaatttca ccgttagtta aagcgccacg
    19501 atcttcaatc cagatttgaa gagaagatga ctcagagcct gttaacggta agccagaatg
    19561 tgagtaatga atgcccaaca gcatcgaaac cttacccggg ccaatgccat ccgaaggttt
    19621 taagtacgtc ttgcctcttc ttgccaacat gatatctaca ccatccaaag gtgtaatacc
    19681 tcgaatgagt accgcatgtg gcgtgcctgc aacatttgta actacgttga aaagcgaatg
    19741 gataccgtag cagagataaa cataagcaac acctccttgc tcgaacataa ccctgttgcg
    19801 cggggtaatc ttcccgttcc atgcatgaga agcccgatcg tcagtaccgg cataagcctc
    19861 ggtttccact ataatgcctg aagttagctt attctcaaaa cgagttacta aaactttacc
    19921 aattaataaa cgagcaactt caactacgtt ggaattaaga taaaatttag gcttaagaat
    19981 cataaacgga tcttgcataa agagaagaat tgataatgcc ccaacgatga aaacgataga
    20041 gaaaagatgt ttttaaaact cctaacccat acttaacact acggtgaaag ttgatggaag
    20101 aggcttcttc aaaatatttt gtcgggcaag taatttcagc aatttcaaaa cctgccataa
    20161 agatttgaga gattatctga ttatcaaaaa caaaatcatc agaattagct aaaaaattta
    20221 cctttcgaat cacttcagca gaaaaggctc tatagccagt gtgatattcg gagagttttt
    20281 gatttaaaat gaggttttga aataacgtta agatacggtt tgctatgtat ttataaacag
    20341 gcattcctcc ctttcgtgct cctgtaccca aaatccggga accaaaaaca acgggatata
    20401 ctccgttagc aattaggtaa gccatggaat ggataagttt aggggtatat tgataatcgg
    20461 ggtgaagcat gattataata tcggcattta gttcaagagc cttctggtag caggactttt
    20521 ggtttgcgcc atatcctttg ttttcgggat gaataaggat atgtttgatg ccaagcttat
    20581 gggcaatctc aacgctgtta tctgagcttt tatcgtccac aaaaacgact tcatcaacaa
    20641 tatcgaaagg gatttcattg taagtgtttt caatagtttt tgctgcattg tatgccggca
    20701 aaacaacgac aattttcatt ttattaatca tatcgaaaat agttagtaaa tcaatagcaa
    20761 aattaatgtt ttatcctgcc aattatagcc aatagagttt tttatcaaga gtataagcaa
    20821 ttttgttatt gtcaagaagc cattctatta cttttaaaac tttttctttt ttcactcttt
    20881 cagtttgtgc tattagatcg ttaatttcaa atgcgccagt tttaagcagc ggctttattt
    20941 gatcgagaat agcgttaaac tcctgctcgt tcaggtcaac cttgttgcgc tcaaggcata
    21001 catcgcattg tccacaacgt tttattttac tctctccgaa gtagtttaat aaagatatac
    21061 tccggcaacg actattggag gtgagatata catttagaga atttaacctc tctaacgcca
    21121 tcttcttacg cattgagtaa ttctctttgg atatgtaaag atctttggcg tctaatctct
    21181 cttctgtaaa aatgatctga ggtttgcttt tgggcgggac aaatgcaatt gctcctaaac
    21241 gatttagctt ttcgagcttt gcaaaagttt gctcaactga aaggctggta cgacgtgcca
    21301 gctcattaat attaaatttg gtaaactgag taaaaagtcc tgtatatgac cttaatatga
    21361 ttttcagaaa aatatcaagg tcggcatttt ctaccctgaa tttatagagt ttttcgccct
    21421 gaagttttac aaatattctt ggcggaaaat gaaggccttc gctcaggagc agatacccct
    21481 ccttttcaag aaatttaagg gaattaaatg ttgagatagg attaaggttg aactgattgc
    21541 aaaagtcgga tatgtcaaaa tcaaatccgg tattttttcc acttcctacg gcaagactaa
    21601 agtaatttcc taaagcgtga taaacacttt ttatcatctc catatccgga aaagaaaact
    21661 caaactggct gttggcatcg agcagatcag aatcctgata aagaattacc gaccacgctt
    21721 ttttcccgtc tctaccggcg cggcccgctt cctggaagta tgcctcgatg ctgcccggca
    21781 aatcaacatg tactacaagc ctgacgtcag gcttgtcaat acccattcca aaggcatttg
    21841 ttgctacaat tactcttgga tggccagaca tccatgcttt ctgtttcttc tcacgctgtt
    21901 cattctccat gccagcatgg taatgatcgg cagggattcc gtttttattt aactcccgtg
    21961 caaattcgta tgtgcggcgg cgggttccgg catacacaat ggcgcttcca tgtactttct
    22021 taaacatctc aacaagctta cccatcttat tttcttcttt cactactatg taagcaaggt
    22081 tcttccgctc aaagctttgc ctgatcaggt tgggtgtgga gaatcctaat ttttcctgaa
    22141 tatcatccac cactacaggg gtagccgatg ctgtaagcgc gagtactgga gcatctggta
    22201 taatatgacg tatttctgca atttttaaat atggcggtct aaagtcataa ccccattgtg
    22261 agatgcaatg tgcttcatcc acggctatca ggtttacctt catataacga aggacttctc
    22321 tgaaatttgg tgttgcaagt cgttcaggtg aaatataaag gaatttggca ttgccaagtg
    22381 tagcattatt caaggcaagc tcgatttcgg tttgatgcat tccggagtat attgcgaaag
    22441 cgttaatccc taatgccttt agtctgtcca cctgatcttt catcagagct atgagtggag
    22501 aaatcacaat acatataccc tcttttgcaa gagccggcac ttgaaagcaa atggatttcc
    22561 cgccgccggt tggcattaat gcaagggtgt cttgcccggc caaaaccgac ctgataatat
    22621 cttcctgcat cggcctgaaa ccgctgtaac cccagtattt tattagaatt tgttggatat
    22681 ccatcgccaa acgttaacag gcgaaattaa tcattctttt tcaacctaaa aggacatatt
    22741 gctgattatt ttatggtaat ggaaatctta ttaagttttt tggacaatct aaagggcggc
    22801 ggttttggta aatggcgctg gaatgcttca tctcacccaa gcgatataga aggattgata
    22861 aaaaatgtat gtaaaaaatt agcttaacgt attagattct gctgtttttt ccttcctgtg
    22921 gataggaaat atttaaaagt caatttttac gagtgaagca aataaaaaaa tgaaaacata
    22981 tagtgagtaa agcgttgact gcaacaaacg ctctactgag agcggcaata accgtttcga
    23041 tttgaagatt tatggaaagt tcaagttttg tacttcgttt gaaaggtttg gatgaaaata
    23101 acctcttcgg caagacgtaa accgttgtaa aataacgagg ttaaaccagt aaatgacaga
    23161 tctgtgattt gaaaaatgga gagtacttca atagaacata aaatttctca tttccattca
    23221 tgcctgccca tcgaattttt caatattttt gtgcggtttt cttaatttct acctttatat
    23281 gctgggtatt ttctttttaa tacttcttgg attgtctttc ggagtattct ttttccgaaa
    23341 aaaatcaggt tatctctact cgctactatt ggttatgggc atggtagcgc tcactacaca
    23401 tggggcagtc caggcattaa atggcaattc gcaatccttt gtacatggtt tcaacaacat
    23461 attccgatct gccacagaca tattagtgtt ggatggcctt tcagcctttt tcattttagt
    23521 gattaacatt accttattgg ttggactgct ttatgcaaat ggctatctca agccctatca
    23581 ctcccgtatg ggcaatttac ggtttgccgt ccattatttt tcctatatct ggctctactt
    23641 ttccatgctg ttagtggtga tgctgcgcaa tggagttcta ttcctaattg cttgggagat
    23701 gatggccata tcctcatttt tccttgtgat tttcgatgcc gataatcgga acatactcaa
    23761 aacaggcatt aactatttag tacagatgca tgtgggtatg cttttcatat tggttggttt
    23821 tctattggtc gaaaaatcaa cgggaagcat aagttttgat tcattgccca catattttgc
    23881 aaatagcagt aattggccac ttttcctact cttttttata ggctttgcca ttaaggccgg
    23941 atttatgccc ctgcatactt ggctacctga agcacatcct gcagcgccct cgcatgtgtc
    24001 gggggtaatg tcgggggtga tgattaaaat gggcatctac ggaatattca gggttgtact
    24061 tgcaacacaa agcgatttgc tgattattgg tgtgcttctt ctggcagttt cgttaatttc
    24121 aggtctttta ggggtaattt atgccattct gcaacacgat ttgaaacgac tattggccta
    24181 tcatagtatc gaaaatattg gcattattgg gattgggttg ggattaggaa taatcggtaa
    24241 ggcaacaaat aatcaaatgc ttatgttgtt aggttttagt ggcgctttac tccatacttt
    24301 aaaccactcg ttgttcaaat ctttgctgtt tttcaatgca ggttcggttt acctttcagc
    24361 aaaaacccgt gatattgaaa aatgcggcgg gctgatgaaa accatgccct ataccgctct
    24421 cctgttttta ttgggttcca ttgcaatttg cggactacct ccattcaatg gattcatttc
    24481 ggagtatcta atatactttg gaatgattaa taccatgcat ggtgcaggtt tctcacaatc
    24541 tattctattg ctattcagca ttattgggct taccctgata ggtgggttag ccgtgttctg
    24601 cttcactaaa gcgtttggtg tcatcttcct tggctcaccc cgaagcaaac aggctgaaaa
    24661 tgctatcgaa gtgacacata gcatgattgc accgcaaatc atctcagcga ttctaattgt
    24721 tgctattgga gtagttcccg cactatttgt caagccgatt ttcggcatat ttagccacga
    24781 tttgaaaata atcataccgg aatatcatag tttcattgcc aacctatcgg gtatcagcgc
    24841 tgcatcgggt gtgtttataa taatggcagt gggcatactt ttgcttaggt atattcatct
    24901 cagaaggaaa cccatagcag cgggccccac ctggggttgc ggatacacgc tggaaagttc
    24961 tagattacaa tatacagcat cgtcatatgc caacagtttg agccaattgg caaaacctgt
    25021 tattcgccga aagataatca ccgagcctat tagtgaaaat gatattttcc ccccatcaag
    25081 agctttcagc acacactccg aagatgtttt ccgagaacag ctaaccaata aacctgtgaa
    25141 taaatttttg gactggatga aaaagattgc tataatgcaa acaggtcaga ttcagcacta
    25201 tatattatat gcctttctgt tcatgttact gatacttatt cttacctatc ttaatttcat
    25261 ttagaacatg ctcggaatca ttctcatcat aatcgcttca ctgttggtgc cgggtgttat
    25321 cattcgcacc aagagcatcg cgtcggggcg caaggggcct gggctatttc aacccctaaa
    25381 agatttaagg gttttgctgc gcaaaggaag cgttttcagt aataccacgg ggttaatatt
    25441 taggattgca ccctcattat cgttggcaag catcgtgtgt gcactcacat tagtgccttt
    25501 tggcaaccaa ggtgcgctga ttacgtttgg cgccgatttc gtgttctttg cctatatatt
    25561 gtcttttggc aaatttttcg ctatcattgg agcactcgat tgtggaagca gttttgaggg
    25621 catgggggct aatcgcgaag ccctatactc aatgatggtg gagcctgcct tttttatcct
    25681 gttaggcact tttgccatgt ttacaggcta cacttcgttt acagatattt tttacggctt
    25741 ccatttaagc gacaataatt atatattgat attcagcgtt atcagtttct atctgctgat
    25801 acagatagtt atgattgaga atagccgaat gcccattgac gaccccaaaa ctcaccttga
    25861 actcaccatg attcatgaag tgatgatatt ggactacagt ggtttcgaca aggcgcttct
    25921 ccacattgga acattcttga aatacggaat ttacggtgcg cttataaccg ccatactcat
    25981 caataccaaa tggagtatct acattcagat ttttgcattt tttgctgtag tagtctgttt
    26041 gggagttatt ataggcttga acgaatcgtt ccgtgcccgt aataaaatgg gtaaaaaccc
    26101 gcagtttgtt ctaacattgt cgggtattgc gctggtggca tttgtgatag ttctgctttt
    26161 gatgggaaaa ttttaaatcc atttgaaaat gcttaatttt ttaatagtcc tttttgccat
    26221 aaatctgata tatgtcagcg ttgctgatag atttcgcatt tattcgcggc tcattgccgt
    26281 tcaggggata ctgctaatgg gaattgcact gattgaattg caagaggtaa gcaccctaaa
    26341 cctgatacta attctggccg aaacgcttat ttttaaagct atcgtggtgc cctaccttct
    26401 attttcaatt atacatcgca caggaatata caaggtgcac aacagggcgt taccgggctt
    26461 cttctctctg atactcacta taattgcact ggtactaagc atagttatcg ctaatatttt
    26521 agagaatccg catattgata ccgcctatct taccattgcg ctttttacca tgtttgtagg
    26581 agtactgctc atcattggcc acaaacttat cttttcgcat atgattggat ttctgattat
    26641 tgaaaatgcg gtttttatgc tctccatggc cgtgggcaaa gagatgacta tgctgataaa
    26701 tattggtatt ctgctggatg ttttcgtgag cgtgttggta atttccatac tgataaatcg
    26761 gattggcacg gagtttaaca agtttgaggt gcaagacctt acatcgctaa agcattagaa
    26821 tatgattctc ttttacttca tatcggctat tatattgagt gttgccacac tgctggttaa
    26881 taaaaggcga actgcccaaa ttatgatttc cctttttgcg gcaattcaaa ttgcctttgc
    26941 ctattacgca tacctgcatc tcaatcaaac agatttatca tattttacct acgatccgct
    27001 gggagtgatt tttctctcgg tactatcaat tatttttcct gcaacgattt accatggttt
    27061 ccgctatttt accaatggaa caacaaaacg gttttattac taccattcgg cactcatcgg
    27121 ggtgatagca gtgataaccg gagcctatct ggccaacgag gccaccgtgg tgtggatact
    27181 ggtggaggcc accaccctat tggcatcggt actaatatat cacgaaaaaa caaaaatggc
    27241 attagaagcc acatggaaat acattttcct ctgctcggtg ggaattgcct tcgcctatat
    27301 gggtatcctt ttcataggct ttggcattga gcagtacggt tttaccaatt tgtcattcga
    27361 ggccattgca aaagtggcat cgcaggccaa cccactatat cttaaaattg cgtttatttt
    27421 tattttcatc ggttacagca ctaagatgga actgttccca atgcacaccg taggtataga
    27481 tgccaactcc gttgccccat cgcctattgg cgcacttttt tccaccgcca tagtgaatat
    27541 gggatttgtt attattttca gaatatatgt tgcactatcg ggtagcgaga tatttcattg
    27601 gatgaaccat gtgcttatca ttggtgggtt aagctccatc gtggtggctg caggctatat
    27661 gctcaaagcc acacaccaca agcgaatgtt tgcctactca actctcgaaa atgcaggctt
    27721 agcagccatt gcacttggaa ttggcggcgc tgggtattac gctgccatac tattgcttat
    27781 actacattca ttcgttaaat cgtctatgtt tttccaaatc ggtcaactta atagggtatt
    27841 caactcattg gatattaact tttcgggtaa atacgtcaaa ttacatccgg tcggtgcagt
    27901 agtgcttatg gtcgggatga tatttctact cgcaatacca ccttccgggc ttttcattcc
    27961 caagtatctt atattcaaag cgcttatttt cagcaattca tggtttgtcc tcatcgctgt
    28021 tgcactgatg atttgcgtgg tggtgtatgc gctcagcgtc agggtgatgc atatctgctt
    28081 ttcggatgca cgtaaatcaa tgcatgatgt tttaccggaa aagattaacc ccatcgaaac
    28141 ggtaacgcag ttcctattcc ttttggtagt ggttgtgttc tgcttctatc gcccaccatt
    28201 ggttgacgaa ctgattcggc aaagtatttc actattgacc cagatgtaga aaatggaagg
    28261 aaaaaaatat attgaagttt caaactccca agcggttgca attaaagata ttccactgct
    28321 cgattatgat gatttctacg attatgtggt ggacgaactg aaaatagata cccaccattg
    28381 cctcaactac tattgtgttg atttcggtag tcgcctttgg ttcgtttgct gcattgcctg
    28441 cgatgaactt aacattatcc gtatttgttc gcacgaactt ccaaaaggcg aaaaatcatt
    28501 acgctccatc actcccgaat gtttccaact ccacatcttc gagcgcgaaa tccacgagaa
    28561 ttttaggata cacttcaaag gacacccctg gttaaagcct gttcgttcaa gttttaagaa
    28621 tgacggaaca aaacttcatg ttgatgctta tccgttcctc gcctttgaaa gcgaagagct
    28681 gcacgaagta ggcgtaggtc ctattcatgc cggaattatc gagcccggac atttccgctt
    28741 tatatgccat ggagagaaag tgttacatct ggagataagc cttggatatc agcatagggg
    28801 gattgagcaa ttgtttgtca agcagaatgg gctacaacgc acaattctta ctgaaagcgt
    28861 ggcaggcgat actgccgttg gctcgtccat agcccatgct caacttattg agtcgctggc
    28921 tgacttgcca atacccgaaa gtctaatgat agagcgttgc attgggcttg aactggagcg
    28981 ttttgccata catatcggcg acacctctgc cctttgcacc gatgttgcct accagcttgg
    29041 acaggtggtg aatgaggcgc ttcgcacaac tattataaac acaacccagt tctggtgtgg
    29101 caataggttt ggaaaaggct tggtacgacc tggtggaagc aactatccgc tcaacgaaac
    29161 agtggttgac gaaatacgta atatcttaaa ggattcgggt aaccgataca ttgatatggc
    29221 tgataggatt tacaccctac ccacagtgct ttcgcgtttc gatggtattg gagaattaac
    29281 ccccgagcaa gccatactta ttggcgcagt gggcatggca gcacgttcaa catcgttggg
    29341 tagggacatc cgaaaatcgc atccattcca atattttaat caatatcaat acgagcctgt
    29401 tgttttaact accggcgatg tgctggcacg tggtatgctt cgatacgaag agtcgaaaaa
    29461 atcggtttcc atggtcaacg agttttgcac tatatgggaa gggatggcaa aagaagatac
    29521 cccaagacct gtttacgatt atggtttaaa gccattatcc tttgccatat cggttgtgga
    29581 ggcgtggcgt ggcgaactat gccatagtgc aatcactgac gagcagggga acttagctca
    29641 ttacaaaatc aaagaccctt cgctgcacaa ctggatggcg ctggccttag cggtgcggaa
    29701 tcaggaaatt tcagatttcc ctgtttgcaa caaatcattt aacctaagtt actgcggcaa
    29761 tgatatctag aaaaaagcta agaataaaag aagccaatat cctccgtagc cacgggtggc
    29821 agtatatccc cgatttgaag ggtgttgagc tttcgcccct attccgggga agacccgtta
    29881 tcagcgacac tattacccca gaagaaattg agcatgtgaa aaatatatgc cctgtggatg
    29941 cttttgtgga tgatccattc gggttggata tgggaaaatg catattctgc aaggagtgcc
    30001 actttgcttg cccacagaaa atcatgttca ccaacgacta taaaattgct gcctctaatc
    30061 gcgaggattt ggtaataagg gttggtcaaa cgaagatgga tttcgaccat tcggctgtca
    30121 gaccggtaat aaggaaacta ttcaaacaat cattaaaatt acgacagatt tcagcagcgg
    30181 gtgacaatag tagcgaaatg gagctgaatg ccagcgggaa cgtgaacttc gacatggggc
    30241 gttacggcat tgaatttctt gcctcaccgc gccacaccga cggaatcgtt atcacagggc
    30301 ccatatccga aaatatggcc gatgcattac aaatatgcta cgatgcagta ccagagccaa
    30361 aaattataat ccttgcgggt agcgatgcta ttggtggagg tatgtttgtg gatagtccag
    30421 caatcaaccg tacttttctg gataaatacc acattgacct attcgtacca ggaaacccga
    30481 tgcacccgct cacatttata aatggtgtgc taagtttgat tgggaaatag taaagggggt
    30541 taaacctggc tcaaactaaa gatactgtct gtcttgatta taagcagaaa cttttagttt
    30601 taagagatac aattggttaa accaaaaaag attcactgtt tttatcaaat aaagttttta
    30661 tgtttacaaa atttataaag attctaattt atctctttta tatagataat cagtgttttt
    30721 gtaagtttaa ataaaatgtt gttgatttaa aagaagaggt tagcaacatt atgtatctga
    30781 gtattgatta gttttgtatt gttaatagtt taacaaaaaa ataattattt tatgaacaca
    30841 attgataatt tattcaggaa agatgcccta tcttatcatg cagaaggacg tcctggcaag
    30901 cttgaggtta ttccttcaaa gccttataac actaagcgtg atttatcgtt agcctatact
    30961 ccgggggttg ccgatccttg tcttaaaatc aaggataatc cggatgacat ttacaaatac
    31021 actatgaaag gcaaccttgt ggcggttatc tcgaatggca ctgcagtatt ggggttagga
    31081 gatatcggaa cgttggcaag taagcccgta atggagggta aaggttttct atttaagatc
    31141 tttgccgata ttgatgtttt tgatatcgaa attgatctaa aagatcctca aaaatttgtt
    31201 gataccgttg tagccattgc accgacattt ggcggtatta acctcgaaga tatcaaagct
    31261 cccgagtgtt ttgagattga agaaaaaata caggctgctg tagatatacc cgttatgcat
    31321 gatgatcaac acggaactgc cattataact gcagccggcc tgctcaatgc tgtcaagctt
    31381 acgggaaaaa aaattgaaga cctcaaaatt gtagtcaacg gggccggcgc ttcagccatc
    31441 tcatgtacaa ggctttatat taaacttggc gttaggccgg aaaacattgt gatgatagac
    31501 agtaaaggtg tactacgtaa agatcgtacc gacctgaatt atgttaaaca gcaaaatact
    31561 actgatagag atatttatac acttgaagag gcaatcgaag gtgccgatat gtttcttggc
    31621 ctttcggcag caggcgcgtt aaaaaaagaa tatttaaaaa agatggcgcc taatcctatt
    31681 gtattcgccc ttgctaatcc ggtacctgaa attatgtatg acgaagcaat ggctgtacgc
    31741 gacgatctga taatggctac tggccgttcc gattatccta atcagattaa taatgtcttg
    31801 ggatttcctt ttatttttcg aggagcttta gatgtcagag ctacatgtat taacgatgaa
    31861 atgaaattgg ccgctgctta cgctcttgcc gaacttgcta ccgagcctgt accggatgag
    31921 gttaacgttg catatcatgc aaccaatttt gctttcggaa aagattatat catccctaaa
    31981 cctttcgatc caaggatgat tacaagagtt tcatcggctg tagccaaagc agctatggat
    32041 tctggagtag caagaaaacc catcactgac tgggaagcct acaatgagtt tctgctgaag
    32101 aaaatgggaa ccaccaatcc tattactcgt caaatcagac atcgggcttt agcaaatcca
    32161 aaacgagtcg tttttgccga tgccgaaaac tataaaatgc tgaaagctgc tgagtttgtg
    32221 ctgaatcaaa aatctgcata tcctgttttg ttaggagatg agaaaataat aaaggctctt
    32281 attcaacaac atgagttgga catccctaac cctgaaataa ttgatcctcg atctccagcc
    32341 gaggatgatc gcagaaagca atttgctaaa ctactttttg aaaaacaaaa ccgaaaaggg
    32401 attactaatt ataccgctct cgacaagatg ttacaccgca actattttgc cccaatgctt
    32461 gtagagactg gttttgccga tgcaatggtt tctggcctaa ccaacaatta ccccgatgct
    32521 ataaaaccag caatagaagt aatcggaata aaacaacaag caaatcttgt gtcgggaatg
    32581 tatattatca ataccaaatc aggtccttta ttttttgccg attgcaccgt taacaaagaa
    32641 ccgacttccg agcagcttgt ggaaatcacg ctacaaactg catttgctgt aacacagttc
    32701 aatgttattc ctcgtgttgc catggttacc tattctaact tcggttcaaa taaaggcaga
    32761 atacctgata aagttgccga ggcagtaaga atcttacatc gggagtatcc caagctaatt
    32821 gtggatggtg aaatacaagc tgacgtagcg ttgaattccg aaatgttaaa tgagaacttc
    32881 ccattttcaa aacttcaagg caatcctgca aacacactta tcttcccata tcttaccgcg
    32941 ggaaacattg cttacaaatt gcttcgggaa attggaaaat ttgatgtaat aggccctatt
    33001 cttaccggaa tgaataaatc agtacatgtt ctgcattttg gtaacagcgt tcaggaaatt
    33061 ataaatatgg taatggtggc tgcgttagat gcccaatgcg tgcaaaatag ggaattgaat
    33121 aaatgtaagg acgattttaa ggaaatattc agatagcaaa taaaataaac ccaggttatg
    33181 tgttgccaaa tgcttaatct gggtttaaaa tgttacgagg aaacaatatc tttacgtggt
    33241 ttcagttttt ttttaatctc ttctaaagct ttaaattggc ggagtttagc taaatttaga
    33301 aagcaaaaaa aaaaccgttc aggggtgcta aacggctttt ataaagtaaa gtaatgatgc
    33361 atcattatct tgatattaca agacgtttcg tgtcaatcac ttcattattt accacaatcg
    33421 aataaaaata gaaaccttcg tttagatcac atacatccaa aataagtttt cctgaaaaat
    33481 aatcaaggcg tgtttcttta acaaccgttc ctaataaatt gtgaacacga atataggcgt
    33541 taattgtttg atccgatagg gtatagtcaa acgatacctg attgcttgcg gggttaggat
    33601 aagccaatga aaatgagttt ttagtgtttt ttgtcagatc atcaatacca actgggccag
    33661 cattaaagtt aacgaaaaag tgggtggagt catttggatt atcatcatca aagaaggtgt
    33721 atctgataat ggtaactccg acattattga atggcatata atcacctgag aactcatcgt
    33781 tagtaacgcc ggggtcaata gttatacctg caggcgatac gaatattgtg tttgcccaac
    33841 acactaccca gcaaaatgtg ttcattgaac ctggtacaac ctgaacctct tctttcttta
    33901 ctttaacaag tttggcagca ttagaaatgt tttttatact gatgtgcgct tgcattaaag
    33961 aggctgatgc ctcgtcaact acgtatatag tccctccaaa cggaaagatt tcattttcat
    34021 agtaaagctc aaagctttga ctaaaagcaa atggaatgat aagaagggaa gcgattaagg
    34081 aaaatataag ttttttcatt gttttaaaga ttttaagttt cgaaaaaaaa tatcaaaaac
    34141 cgtgcaaaac aatctaagtt tatttcaaat attatgagaa caaaagtagt tatataattt
    34201 aaaccaatgg tgtttttttt taatatgata ttttactatt tttgacattc cattgattgg
    34261 ttcttttttt ttgatttttt tacattataa aaatggtttt ttctacagta atacaggatg
    34321 gcaaaatctg atcatacaaa ttatcaactt tttgatgacc cgattcgttt ttacaacgtc
    34381 atccttgatg atattgcatc ggcgaaaaag tatgttttta ttcaaatgtt caagttcagt
    34441 catcaggcaa tgggcatgaa gtttagggat tcactcactg cagtagcatt aagaggtgta
    34501 gaggtcaaat tattgataga ttcatggggt ggcagctcct ttccttacaa tttcttcgac
    34561 gagttggtat cagcaggtgg agaggtgaga ttttttgaaa agattaaaat caacattgac
    34621 attttcacgc ggagccaccg tcgcaaccat cgaaaactaa taattattga tgacataatt
    34681 tcatatattg gttcttccaa catttctgat tataacttga tttggaggga gagcatgctt
    34741 agaatttacg gatccatagc cgttaagttt aaaaaagttt ttaatcagga ctttagcgct
    34801 tataaaaaat attttcgtta caaagagagt ttcacgagaa ttatcaggca tggcgatatg
    34861 gaaattttac gggattcgcc ctcagtaacc cgccagaaaa taaaaaaaag gtatgaggag
    34921 gttataaaaa atgctttaaa agaggtgatt attgttacac cctatttcct gcctggtttt
    34981 aatcttcgaa aagtattgat ggacgcagcc aacagggggg tagaggtaaa agtgattatt
    35041 cctaagcggt cagatattcg tatggttgat atcttgcacg gtcgttatct tgaaatttta
    35101 aataacagta acgtaaagtt tctctatttc acaaagcata atcttcatgc aaagctgcta
    35161 ttggttgata atgaaaaatt ttcgataggc tcacctaatt ttgattatcg tagctttaga
    35221 tatatgcacg agatagtact ctttggtaca accccgtcta ttataaaatt gataagggaa
    35281 ttcataattg atacacagca atattgcgag ccttttgact atgaagcgtg gcagcgccgg
    35341 ccggcaatcc agaaattctt tgaatgggtg ctcttaccct ttcggcatct cctttaacta
    35401 ttttattgta taattttgcc ggctccttta agataactga tatggcaact caaaaagaga
    35461 taaaaaccaa atcgaaaagc aggaaaaaag agatattacc tttagataat gaaggtgaac
    35521 ccacaaaaat ttttgtgctc gatacttcag tgattttata taatcacaat tctattaaca
    35581 gcttcaaaga acacaacgtt gctattccaa ttacagttct cgaagaactt gatcaattta
    35641 aaaagggaaa cgatactata aattttgaag ccagggagtt tatcaggatt atggacaaac
    35701 tatcggccaa tgcttctttg caaaattgga tatcattaaa gaaaaaggga ttaggcaagt
    35761 ttaaagtaat aatgaataac agcgctaaat ccatggatgc taatgaggtt tttggagaat
    35821 cgaaatgtga tcaccgtatc ctgaatgcag cacttgcatt gcagcatgaa catcccgaac
    35881 gaaaagtgat tatggttacc aaagatgtaa acctcaggat taaagcaaaa gctgttggat
    35941 taacagcaga agatttcttg actggaagga ttgaaaatat tgactctctt tattcgggaa
    36001 tatcacgatt ggaaaatgtg gataagagag ggattgacag gctttatgaa gaaggattgt
    36061 gcgaagtaga agatctgaat attgaaaatc ccaagccaaa tcaatatttc attttgcaaa
    36121 cagatggagc atctgcgctt gggtattata atcctcgtaa ccagcttatc gaaagagtgg
    36181 ataaacatac tgtttcgaaa ataaatcccc gtaacgccga acaggttttt gctttacatg
    36241 ctgttaccaa tccgaatata agacttgtta ctttacaagg gattgccgga acgggtaaaa
    36301 cactacttgc ccttgccgga gcatggagcc aacgaagaaa ctacaaacag ttgtttcttg
    36361 cccggccaat agtgcctctt agtaataagg atataggttt tttaccggga gatgctaaac
    36421 ttaagatcga accttacatg cagccgttgt acgataattt gagatttatt caaaatcaat
    36481 tcggcgagca tgataaagag tttcagaata ttacggcagc catgcaaaat gaaaaattgg
    36541 tgatacaacc attagcatac attagaggca gaagcatctc caatgtcttt tttattgtag
    36601 atgaagctca aaatcttacc ccacatgagg tgaaaacaat aataacccga gccggcgagg
    36661 ggactaagat catctttaca ggtgatattt atcaaattga cactccatac ctcgactcgc
    36721 aaagtaacgg attgtcttat cttattgata gggtaaagca tcataactta tatgcacatg
    36781 ttcgcctcga aaaaggagaa cgttccgatt tggcaaatct ggctaatgag atgctgtaga
    36841 tttttgtgcc acaataaagc cgtttcatta atgaactttt tataaccctt tgaaaacgac
    36901 atttaaaacc acatcgccaa ccatttttaa agtgttggtg tcaatatgat ccatgttgtc
    36961 gtttattgta tgccagtgtt cgaaaaatga accgttggag gatgattgat ctaaatgaat
    37021 aatatttatt gttgggatac gtgcaattcg atttataaaa taatgatcat cggtaatata
    37081 accgccattg tcgaatacaa agcgatcgcc gtatccaagc ctaccggcaa tttcccaaac
    37141 ttttttacca atgtcgggag cataatacat tgaaaaccct tctaatggga actgtgcatc
    37201 aggagctcct accatgtcaa gtaaaattcc aaaattggcc gaatagtgtg ttgtgtgagg
    37261 attccttgac caaaattgcg aacctaatcc ccagaagtca gtagattcat acgcttgcct
    37321 atcctgtggc ggcccataat cttcaatgtc aaaaaggact atgtcaactc cagcggatgg
    37381 actgtttaag ctcagttgtc tggccacttc catcaatacg ccagtaccac ttgcaccgtc
    37441 attggcacca tcaataggtt tttgatgatt ggccggatca gggtcatgat ctgcataagg
    37501 cctcgaatcc caatgagcgc aaagcaagta ccttggttgt ttgttgggat taaaaacggc
    37561 gataatattt tttccattca atatttgatt gttgtaagcc cttgctttaa attcctgcaa
    37621 gctgacatgc gaacaaaatc tttgtaaatt ttcttttatc cactcagcac aattgctatg
    37681 ggcttcgctc tccggaaccc tggggccaaa agaaatttgt ttttcaataa aaaaataggc
    37741 tgagtcagca ttaaactccg gtacttgaat agaaacgtta gtgggagtgg gatcagaaga
    37801 tttttttgtt tttttgccag ctttgttgtt gttacaagat agcataagta aaagcaaaag
    37861 taaaatggag agtaagcgaa tgttttttaa cttcattgtc tttaaaatta ataaaaaagc
    37921 cactgattaa cctaacgata atttcattcc aaattttccg gagaaaagga agtcgttctg
    37981 attttttcgt gtacgttctt tcagttcaag ttctttaaga ttagggtgta attcgttata
    38041 gtcgccaatg taacctattg tgaatacact aattggcacc tgatcatcag gtatttcaaa
    38101 atcatttatc aatacttcgg catcgaaacc gcctatctga tgtacaaata gtccttcgtg
    38161 catagcctgt attgatagat gagcaacagc ctgccccaca tcataggaat accaacggtt
    38221 ctgtttgcca gattttttag aaattttttg cccgatcgaa acaaccaata taggtgcaaa
    38281 ttttacccat aattgattga attcaacaag tgagctgaaa attttttgat acgtttcatc
    38341 attgtcaata ccaacgataa aagaccaagg ttgatcatta ttggcagaag gcgcccaccg
    38401 tgcagcctca aagatacgtt gaagctggtg agtatcaaca ggcttcaagc taaaagcacg
    38461 cgggctccag cgacgggtca acaaagactg gatgggaact ttagtaattg cgggtttttc
    38521 catacaacta aatttttaaa taaatgcagt gattttttaa gttaataaat gaatttagat
    38581 tctacttaga atcgtttgca aacttataga aattaagtaa ataaatattc aaattaaaac
    38641 agagattaag ccgtaattta aaaagtttga aagaaaaaat ttggtcgcta aaatgaagtt
    38701 gacaagtttt tgtttctttt caagaaaaca gttcaaatat tgaatttgaa actgattttg
    38761 tcgattaatt aacttattat ttctgaaagg tttcaaaact ttaaatttgc aaataaatat
    38821 ctgtaaatat gagattagca ataattggct caaccggaat ggtaggtcgt aaaatgctgc
    38881 aagtacttga ggagcgcaat ttcgtcattg acagtttgat tcttgcagcg tctgataaat
    38941 cggttggaaa taaaattatt tttcaaagca aaccgtttga ggttgtttcg atgcaggagg
    39001 cttttgctca gaaaccacat attgcattgt tttcggctgg ttctacggtt tctcaagcgt
    39061 gggcaaagcg ctttgtcgag caaggtactt ttgttattga caactcatcg gcctggcgtt
    39121 tgcacaatga tgttccctta attgtaccag aagttaatat agatgcgttg caacctaata
    39181 agccactgat cgccaatccc aattgttcca ctattcagtt agtcgttgct ttagcgccac
    39241 tgcatagagc ctttaaaatt aaaagagttg taatatctac ttatcagtcg gtaaccggct
    39301 cgggagtaca tggcagcaat caattgagat gtgaacgcgc taatttacca acaattccat
    39361 tctatcctca tcagattgac ttaaacctga ttccacaagg aggtagtttt aattctgacg
    39421 gttatactac cgaggagcag aaacttatag atgaagtaag gaaaattttg tcggatacct
    39481 ctttacaaat taccgctact gtggtaagag ttccggttta tggtggtcat tcggaatcag
    39541 ttaacattga gttttataca aaattcgaat tggatgaagt taaaaggctt ttaaaaatga
    39601 tgccgggcat agttattcaa gatgaacctg aaaaatctat ttatccaatg cctttttatg
    39661 cgcaagaaaa agatgacgtt tttgtgggtc gaataaggag agattttagt gtcgaaaatg
    39721 gtctgaatat ttgggttgtt agtgataatc tgcgaaaagg agctgcaact aacgccgttc
    39781 aaatagctga atatatcgta agaaaaggat ggattaaagg ataatattaa aattcgttgt
    39841 tgttgtttta ttagtttaca aaaaactttt tcacgtaaac atcattattc aaataaatcc
    39901 tggcaatgaa tattccttta aaaggataca tatctattga atatttattt gctccggcca
    39961 actggcgtgt tttaattagt cttccagcta catcataaat atctatggca gcgtatgttc
    40021 tttcggggaa agaaatgtaa atgttattgt tatatgaata aaggtgaatg taggcgaaat
    40081 taagcttagg aacatatgtg atatctgcga tgttgatatc atctaatctg atcctatggc
    40141 ttttgccata attgcccgaa aagtaataaa atttccagag caattgtaca tattgtttat
    40201 taaggatata gtcaggcaac ggaatatcca agaagttcat tagtgagcca tgcatttcag
    40261 gattgtattc caaaacttta ttttttatta ttaattccct gaaaggttct gtaacattaa
    40321 agcgataaaa caatctgata gcatattttt tttggttttg ctctaataat tctgcagtcc
    40381 agttgataaa accgaaattt acttcacgtg tatccagtgc aatgaggaac ccaccgaggt
    40441 ctctttcggc atttgtatta actatggcta tccctttatt gtcaagtccg ttgattcggg
    40501 tacgataatt atttttatat ggaaatccga tattttgctc atcttcaggg gcataaacat
    40561 tgtgtggaat aaaatatgca aagagcaatt catcatatag atccgggtca tttttatcac
    40621 tttgtaaaaa gatcatgtgt tcagggaatg aattttcggg ttggttttca tcccatttat
    40681 taaaagatat cttgttttta cgtaattcga atgctttagg ataaacagta ataataaaac
    40741 ttgaagcgac tggttctgtg aaacaatcgc ttacaaggat gatgatatta catgcacctc
    40801 tttggattgg caaaatagta agtatcgaac catttaggtc tgtttgtgca acctgcggat
    40861 gcatattgat aaaagaataa tagatagcat cgccttcagg atccatcaca tatttatcca
    40921 aattaataag aattgcctct tcgttccctt ctatacattg ttgtaaaggt atttgagcta
    40981 taaataccgg aggtgaatta tcgggtgtta tgactattcc atcgaggcta atattattaa
    41041 aagttacacg gttcccgtca tcagcgctca tattatcgcc gtcaaaccga attctgattc
    41101 caaaatcagg attgtcgttc gttgcttcaa tgtttttaaa atcaaattca aaaagttgat
    41161 attcaccaga tagcaagggt gttggatttg ataatccttc tgatgtccaa ttttccggtt
    41221 cagtatcaat ggtatattca atgataaggt tgtcggcagc tccctcatct ttagcagcaa
    41281 acctgaaaac tacatctctg attcctgtag ttggcagttg gaagttcatt atgttttcgc
    41341 ctccatcgcc aatgaatggc tgccttacct gtattccctt agtgttactt aaattgtatg
    41401 ggatattatt gtttccttcg ggccggtagt taatgtttgt tggttcattg cgtctttcca
    41461 tcgaagcctt ccgccaattg ggatgaccgg cataaaatgg atatccgtct atggaagagt
    41521 gaaacgttaa cgttgcatca tttaaaagcg aataagaggc ttctattact tcaagcggag
    41581 tattattggg aagcgaagtg tcaaaaaacc aataatttac aagcgttgga gcattatttt
    41641 ttttgaaatg agctttaaga tataagtttt tagcaggaag taaatttaat attggatctg
    41701 ttgtagtggt gtcgcctgac caatgtgtaa atacataacc cggcagcgct atagcctcta
    41761 cctctatagg aataccaagg taataattcc ctgaccatgg ataggcgctc tcattgatgc
    41821 caggcgtatc atcagtaatt tcgatactgt ttattcttac tttaccaaac caatggtttg
    41881 acacgtcaac ttccaatgaa tactggcctg acaaatcaaa aaaatcaatt atatgctgat
    41941 tctggtaggc agggcgttgt tcagcaaaat gatacattac ctccatttgc tccagccaaa
    42001 gttgatagct tgaaatgttt ttccatcttg tgatatgttc gctaacttca atgtctatct
    42061 tattttcagc ttccaatatt ttgttttgca tgacggaggg taaaaaggta gtatttaaaa
    42121 ggtctgcaaa acgatttata aagcttatct taaattcttc gttttccaaa aatttacgta
    42181 gcaagaaggt agcccagtca ggatttggat attctaatcc tccagcagca gttgcaaatt
    42241 gcagcatgtt atgttgtaaa gcctcttcta agccaaaaag accaaatcca aaatcaagat
    42301 cttttacaaa ccaacgccaa cgtccatctt gcccggtagg cgcatttggg ttatactgtg
    42361 atgttctcat tctccaatat tccaaattgt tacccggcca atcatgatta tcagcaaaaa
    42421 tatttatgat ctggtaatct ctgaaattat caacatcaat acgggtttgt atatattcat
    42481 aatgctcttt ttccgcaaga ccatgctgtt taatatattc ttttgtttcg ttgtaatgaa
    42541 ccgtatcgcc ttctcttacc tcattgtagt ttacgataaa atcaatattt ccggcatcaa
    42601 tatcatacac tcttttaagg taatccttat caaatctctc tcgtaaatta tgtattcccc
    42661 aatactcgcc atttatgaat aagatggcgg gcattacctc ctgtatgtcg ggtattaaat
    42721 ctttaaccat ttgttgaatt gcagcatctc gtaacatggt ctgaaaataa tcctgaccgg
    42781 aatttcttag gatcaaggtt ttaaactggt catctttaag atttttaaaa aatttataat
    42841 taaaattagg ttgaccatac tcgctacgtg aatacagccg tattgatttt aaaggacggc
    42901 gtcggctttc gtacccatta atacgaaatc ctatttcatg cgaaaaagca gaaaaattgt
    42961 catttacgtc aaaaaactca aggttagatc tcttttctga ttcaatacca attctatgga
    43021 aattggcttt tgtctgccag tcaataggac tttcgggatt agccattcgc cactcgtcaa
    43081 aatcctgtcc agcagtataa atgccgatat tataatcaaa caagtcattt tttccaacgc
    43141 ttaacgaaaa aataggcaag ctgtaacgtt ctcttccaaa actagtaaca aaataagtat
    43201 gtgtaatagt tgggcttttt atccatcctt ctttaaatgc catcgcccta actaatgttc
    43261 ctttaaatac tggattttca gggaagtaac aaagattatt atcaaaggtt gtggagatat
    43321 gggtaatact gtcgggtctt gcgcaaatat tttcaatatg cagagaatcg ttgaattgat
    43381 atgtttgaaa cgttgatgtg aggaatttgc caaaaggatc tctcggatta aatggatatt
    43441 tatctttata aataaatgta aaaacgttca tatttgctgg attcggctct gatccgtcca
    43501 gggtataata aataattact tcggggtcaa tatgggagat ggtgagccaa aaatcgtcgg
    43561 tataaaaacc gccttgatgc gaaaactgcg gatgatccag gataccagga taggcttgtg
    43621 tttggtttgg agcatttggt gttggctcaa taaagaaaaa ccatttgtca ttaccatcag
    43681 gctgtctgcc gtaagaaatg tcagaaggta aagttatcgg cgatacttcg caaattgttg
    43741 tgccataggg attggttaaa atcaatggtt catcggaaac tttaatactg aaattggtat
    43801 gcaatggcat accgggtatt gttttatcct tgcctgacgc ccaaactaaa atatactgat
    43861 cgggtaaaat tgtaatagca ggaaatttcc aacgaaatgg attggaatca tcatcagaaa
    43921 ggccaaatcc aaaaagattt atagtttccg ttccatagtt atatagctca atccaatcag
    43981 aatagtcgcc gtcttcatcg gtgatgaagt aactattggc cgacatgaat tcattgatag
    44041 taatggattg tggatagagc cgcagcccaa ttatcggaag aagaattaaa aataaaaatg
    44101 atttgttaaa ttgccattgc acgttaaacg gattaaacag aagtataaaa ccagttttta
    44161 tgtctgtaac tgattatctg agtaaaattt ttgttgaaaa attattttct gaagtaatta
    44221 atctgactac gaaaactccc tttatcattc ctaaatcaac aacacttgta gtggagtttc
    44281 tgattttatt cgtttttatc aacctgccca tcacatcata caccataata tctccattga
    44341 caatggtagg ggaggttaat attatctcgc catcttttgt atagatatca aatttagctt
    44401 tttgactttc gttaatgcct gtaacatttt taaaaacaat atcatctaat cttagttgcg
    44461 ctcttgaact tgtatcaccc tcaactaaat aatatttcca taaaaactga acatattttt
    44521 ggttcataat attgtcaggg aggctgatat cactaaagcg ttttacatcc ccttgttgag
    44581 cggtaacata ttcaatctct tgcccattat gcattaggtc ggcaaatggt gtaaagaggt
    44641 ttgtccgata aaaaagtcgg atggcatata ctctgttatt tcttataatt gtacctccaa
    44701 gccagtctag cttggcagat tctacttcgc tcaaatctac tgcaatgagt agcccgccaa
    44761 ggtcgcgccc gcggccggta tttataaaag caataccatc gtcatccaat ccattaattc
    44821 ttgtacgacg gctgttttta taaggaaagc ccaaattagc caaatcatca ggatgatagt
    44881 catcgtgagg aataaaataa gggaacaaca aggcatcgtt tattttagga tcattctgtt
    44941 cggattggag aaacatcatg tactgaggat aagaatactc cggttcatta taattccagt
    45001 atgtaaaaga gagctcacca tccttcaaag gaaaagctcg tggatagaca agaactcgaa
    45061 atgaggtctg tactgtaggg ttgaaaccat cgttagctgt tatagttatg attgcatcac
    45121 ctcttttcaa cggtgtaata cttgttgttg aacctgtcaa cgtagcttgt accattgttg
    45181 gtcgctcgct gacaacacta aaaacaagag gatcattatc gggatcgcta aagatattat
    45241 tcaaattaaa ttccattcca tcaccttgtt ctattaactc ctgaagttcg atattgttaa
    45301 ttataaccgg cggtaaatta ttacccgata aagctgatgc atcaatacta aagttgttga
    45361 atgtaactct tttcccctca tcaacactca tgttttctcc aataaatctt attcttatct
    45421 taaaatgctg attattactt gcttcattga tttggttaaa gtttaccaca tacaattgat
    45481 aattatcgct aagatcaggt gtgggattgg ccaaacccgt tgttgtccat acaggttctc
    45541 ctgaattaac cgaataatca atggataatt tatcggcagc gccttcatct ttagctgcaa
    45601 actgaaagac aatatcttta aacccattag ttggtaaatg gaaatataaa gtattttctc
    45661 cgccatcacc tacaaaaggc tgtttaacct gaatagcacg catattagaa acatcataag
    45721 agattccgtt gttaccttct ggacgatagt ttagcggtgt tggagcattg cgccgttcca
    45781 tcgaagcttt acgccagttg ggatgatcgg gggaaaaagg atagccggat aatgcagaat
    45841 tatacaaaag tatagcattg gggttaagat tataaatcgg atcaatagcc tctaaaggcg
    45901 tatcgttggg tagtgatgta tcaaaaagcc aaaagaagac caagttagag cttggattgc
    45961 tgattgatgt agtacagtta acgctttgac ctgagtgaat caaaataaaa ccaaataaag
    46021 cgataaacac agcaataaaa gcattaaagg ttaatttgtt tacaaaatgt ttcatagtta
    46081 ttttttcgta aatagttaat tagctttgca ttcaatataa atacaaaatt acgttttttt
    46141 aatatcaaca gagtgtagaa cttttgattt aaatgtctta caacttttta tttaatcaat
    46201 aaaatccaga tgtttatgtt tagattcaat cgtttaattt ttctttaatc aaaatctgga
    46261 tttatgcaaa agaatgtatg tacctttgtt gcgcaaataa caatgtttag gcatggcaga
    46321 caatataaaa aaaccagtta gagaactaga aaatgttgta atcaggttct caggcgattc
    46381 tggggatgga atgcaattga caggcacgct gttctccgat acctcagcgc tttttggaaa
    46441 ttatcttgca acctttccag attatccggc cgaaataagg gctccacaag gtactgtggg
    46501 cggggtgtca ggatttcagg tgcatttcgg acatggcgag gtaaatacgc caggggatta
    46561 tgcagacgta ttagtggcaa tgaatcctgc tgcacttaaa gcgaatacca aatgggtaaa
    46621 atctggtgga ataattatcg ttgacattga tagttttacc gaaaaaaatt tccagaaagc
    46681 agggtacaat aatgaccctc ttaccgatcc taaaatgaaa gattataatg tgattcaggc
    46741 gccaatcact tctcttaccc aggaatcact caaagacctg aaccttgata acaagagtgt
    46801 tttacgaagt aaaaatatgt ttgctcttgg tatggtatat tggctttttg ataggcctat
    46861 ggattttaca aataagtttt ttgagaataa atttaagaag aatcctgtcg ttttagaggc
    46921 caacaaaacc gtactgaaag ccggatatca atatgccgaa tcctctgaag cattgcaatc
    46981 atcatatagt gttgctcctg ctaaaataga aaagggtatt tatagaaata tcaacggcaa
    47041 tgttgcaaca gcctggggat tgttggctgc cgccgaaaaa tccaaactga ccttatttct
    47101 tggttcctat cctataacac cggctactga tattttgcaa gagttatcta cccgcaagga
    47161 tttaggtgta aagaccttcc aggccgaaga tgaaattgca ggtatctgct cagccatcgg
    47221 agcaagcttt gcaggtcatt tggccgttac tactacttcc ggaccgggtc tttcgttgaa
    47281 aactgaagct accggcttgg ccgttattac tgaattacca ctggtcattg tcaatgtgca
    47341 acgtggaggc ccttcaaccg gcctgccaac caaaaccgaa caagccgacc tgttacaggc
    47401 aatatacggt cgtcacggcg aaagcccact ggtagtatta gctgccagca ctccttctaa
    47461 ttgcttccat tatgcttttg tagctgcaaa aattgccctt gaacacatga ccccagtcat
    47521 attattaacc gatggctttt tagctaatgg ctctgaacca tggaaaattc cagaaatgaa
    47581 agatttacct gatattaaac ctcctctgat taaagaagga gcagaagatt ataaacctta
    47641 taagaggctt aatgataata tggtcagagg ttgggctgtg cctggcacaa aaggatttga
    47701 acatagactc ggcggactgg aaaagatggc agtaacaggg gcagtatcat acgttcctga
    47761 aaaccatcag gtaatggtta acgaaagaga aaagaaagtt gaagctgtag taaatgatat
    47821 tcctgatctt aaaattcttg gcgaagagag cggtgaatta ctgcttatag gttggggcgg
    47881 tacctatggg cacctctata cagctttccg cgaattacga aaagaaggct ttaaattgag
    47941 ttacatacat ttcaattata ttaagccatt cccaaagaac accgcccaaa ttttgaatag
    48001 tttcaaaaaa cgattggtat gcgaacttaa ccttgggcaa atggcaaatt acctaagaat
    48061 gaagtttgaa ggattgagct tcttgcagta taataaagtg cagggactac cttttaccgt
    48121 aattgaattg aaagaacaaa ttaaaaaaat attggaggga aagtaagcta tgaacgaaca
    48181 agtaaca
//
LOCUS       DAOOAI010000004        41548 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_1195_length_41548_cov_5.385125, whole genome shotgun sequence.
ACCESSION   DAOOAI010000004 DAOOAI010000000
VERSION     DAOOAI010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 41548)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 41548)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41548
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1195_length_41548_cov_5.385125"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(242..2176)
                     /locus_tag="PK345_00635"
     CDS             complement(242..2176)
                     /locus_tag="PK345_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33559.1"
                     /translation="MKKVLLVVLSFCLVTIAYSQKRVPISKELTKMSLTRVYEMPSDL
                     EAPMVNFNYTYPGLMKEAKMLGDEAEIIETIYDLQTNSTVANRFVVWDDGTMAAVCTR
                     GVESPGGFAFPDRGTGYNYFDGSSWGPKPSTRIESKKAGWPSITKWGANGEFVVSHQG
                     GGNPLLLLKRETKGTGAWIESTYNGPNGTAAPQYLWPRMISSGQNNEYIHLFALTAPT
                     GNGGTPYMGQDGALLYNRSTDGGESWDIQHQLIDGVGSDYYLNIRADDYTMAFKGNTV
                     ALLQSSAWKDLFLLKSTDNGESWEKIIIWEHPYPFFDVQSTLTSDTLYSVDQSASLAI
                     DDNGMVHVVWGISRVARLESAPPDPGSYSYWPYTDGIGYWNESMGQIPEADNPHRTMM
                     PEYLEEMGMLVGWTQDVNNSGFIFDFEGTNDTPFNAYRSLGISTMPTIAIEGSMIMVA
                     YSSVTETYVTADGTMNYKHIWTRFSYDLGETWGDFYDLQGDNIFHLYDECIYPILADK
                     TNGSGAFQLIYQADNLPGLYLDEDHDAVVNRVIHNNMNFTVGIEKPANMVSPFKVTEA
                     YPNPATSSTIVGVELDKAANIGFEIFNMTGQKVFELPVKSMNAGFNTISFDVSALTSG
                     IYFYTVSNGAEKVSHKLIVK"
     gene            2701..3750
                     /gene="queA"
                     /locus_tag="PK345_00640"
     CDS             2701..3750
                     /gene="queA"
                     /locus_tag="PK345_00640"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008150013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HPX33560.1"
                     /translation="MKLSQFKYHLPSELIASQPPENRDQCRMMILDRKTKSIEHKNFS
                     DILDYYDDGDVFVLNNTKVFPAKLIGIKEKTGARIEVFLLRELNIETRLWDVLVDPAR
                     KIRIGNKLYFGEQDELVAEVIDNTTSRGRILRFLYDGPYPEFRRMLQAMGKTPLPKAI
                     NREVKPEDEVWYQTIFAKHEGAVVAPTAGLHFTRELMKRFEIKGINFAELTLHAGLGN
                     FRQIEVEDLSKHKMDSEDMNVDEKCVEIVNKAKDDKKQICSVGTTTIKAIESALTISG
                     HLKPYSGWTNKFIYPPYDFKLATSMVTNFHLPMSNMLILTAAFADYNFLMKAYKEAVQ
                     KKYKFFSYGDAMLIL"
     gene            3890..4564
                     /locus_tag="PK345_00645"
     CDS             3890..4564
                     /locus_tag="PK345_00645"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HPX33561.1"
                     /translation="MNKTVIIVAGGSGKRMNYGIPKQFITLSGLPILMHTISRFYHSC
                     KDINIIVVLPPDHIETWSRLCSEHSFFISHRVVKGGSQRFYSVTNGLEYIGTGELVAI
                     HDGVRPLITKELIMHGYETASTFGSSIPVIPLPGSLRKIEGNHSYSIDRNHYCLVQTP
                     QTFQSELIIKAYSQPYRKEFTDDASVYEAAGFQVHLIDGISENIKITLSMDLKIANVL
                     IKDFSQ"
     gene            4624..5187
                     /gene="ruvC"
                     /locus_tag="PK345_00650"
     CDS             4624..5187
                     /gene="ruvC"
                     /locus_tag="PK345_00650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016661416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction
                     endodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HPX33562.1"
                     /translation="MMENRPERIILGIDPGTTIMGYGVISDNGKSVTMLAMGVIKLSK
                     FNSQALKLRRIFERTLGLVEQYKPDELSIESPFYGKNVQSMLKLGRAQGVAMAAALYR
                     DIPIFEYSPLEIKKSITGNGSASKEQVAAMLNRLLTFDKNPETLDATDGLAAAMCHYF
                     KRTKGIKGKTYAGWKNFLADNPDRIKK"
     gene            complement(5234..8929)
                     /gene="purL"
                     /locus_tag="PK345_00655"
                     /gene_synonym="purI"
     CDS             complement(5234..8929)
                     /gene="purL"
                     /locus_tag="PK345_00655"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HPX33563.1"
                     /translation="MICFFSNESSVIALSKNNPLTDNELIRLKWLLNGAEQIISDKID
                     GYFIGPRKEMVTPWSTNAVEITQNMGIKGIGRMEEFKKVENKNIAFDPLLSSLYTGLD
                     QRLFTIDKLPNPILFIEDIALYNETEGLALSKDEVKYLDELSERIGRKLTDGEVYGFA
                     QINSEHCRHKIFNGTFIIDGKEMPHSLFSLIKETSRRNPNHIVSAYKDNVAFIEGPQA
                     EQFAPVRQDVPDFFKTKVIDTVISLKAETHNFPTTVEPFNGAATGSGGEIRDRMAGGK
                     GSMPLAGTAVYMTSYPRLEATRQWEEQFVERQWLYHTPLDILIKASNGASDYGNKFGQ
                     PLICGSLLTFEFNSDDKKWGYDKVVMLAGGIGFGKRIDAHKEKPQPDDLVIVMGGDNY
                     RIGMGGGAVSSVATGQFDHQIELNAVQRSNPEMQKRVYNVIRAMAESDQNPIIAIHDH
                     GAGGHLNCLSELAEETGAAIRIEKLPIGDPTLSDKEIIGNESQERMGLVIKDRDVSLL
                     KQIAERERAPFYQVGKITGDLRFTFQRENSQIHAMDLSLSDLFGKTPKTILTDNIIND
                     QFVELQYDILEIQDYLENVLQLESVACKDWLTNKVDRSVTGKIALQQCAGELQLPLNN
                     LGAVAIDYQGVNGITTSIGHAPVAALIDAAKGSVLAISEALTNIVWAPLRYGLKGVSL
                     SANWMWPARNEGENARLYSAVEAASKFAISLGINIPTGKDSLSMTQKYPDGQKVLAPG
                     TVVISAVAEVSDVKKIVKPVLSNDENTEIIYVPFTKSGFELGGSSFAQILGMLGMTPP
                     DISDPEYFKKSFEAIQNLLNEGLIIAGHDISSGGLITALLEMNFSNLKGGLDIDLTEL
                     CEKDLIRLLFSEKPAVIIQVKKDQEVLSLLKSSGIVAFKIGKYVSLREVRIQNWGTVH
                     HFDIDSLRDIWFKTSYLFDRNQTKPMFALARYRNYKKQPLIFNFSDIFLGKFSQLGIK
                     PKEMGGKKIKAAIIREKGVNGDREMAWALHLAGFEVKDIHTTDLIKGTEGLEEINFIV
                     FVGGFSNSDVFGSGKGWAGALLYNDKANTAIRNFYSRADTLSLGICNGCQVMTELGLI
                     YPDHKEHPKMKNNDSGKFESAFLTVDILPNNSVMLKSLTGSRLGIWLANGEGRFHFPQ
                     DESNYCIPIKYSYQEYPGNPNGSPFGAAGIVSYDGRHLAMMPHIERVIFPWQWPYYPE
                     KRKTDQMSPWFEAFVNAREWLTEKLKA"
     gene            9069..9782
                     /locus_tag="PK345_00660"
     CDS             9069..9782
                     /locus_tag="PK345_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPX33564.1"
                     /translation="MSQNAERFLACFNAIERHLQKNYNNGNHGPFRMLLNTAANKNAI
                     YKRFLDDLYAIGDLRNAIVHSDRFDGRPIAEPVDEIVEKVEEIWNIIQHPDKVNIFEK
                     KVLYCFVDDYLSKALNIMLKHKITFIPILQNNLIIDVLNGNHITYWLANQTTVSTTET
                     TIREVLLHAEYRGNFALISRKESVYEAIEIFKKSYREEPKNRYYDAIIITNHGRKDEK
                     MTGIIVLKDIVTYLADKEI"
     gene            complement(9825..10580)
                     /locus_tag="PK345_00665"
     CDS             complement(9825..10580)
                     /locus_tag="PK345_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPX33565.1"
                     /translation="MKIALITGATSGIGEAIALKFAQKDINVIITGRRLDKLNALKSN
                     ITSNTKANILALKMDVTDRESVEREINGLPDEWKSIDILVNNAGLAVGLDPIQHGNPD
                     DWEQMIDTNLKGLLYVSKAVLPLMIARQKGHIINIGSIAGKEVYPKGNVYCATKHAVD
                     ALTKSMRIDLLGNGIKVTQIAPGLVETEFSLVRFKGDEERASKVYQGYKPLEGKDIAE
                     IAFFITTLPDHVCVNDILMTPLAQANSTIVDKK"
     gene            complement(10649..11047)
                     /locus_tag="PK345_00670"
     CDS             complement(10649..11047)
                     /locus_tag="PK345_00670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33566.1"
                     /translation="MQKNFICPFCSGQLYLNERIVLSAQKHDGTRGLILLTPELGDYR
                     AIKHPTFKIIAGEHIDFYCPLCHKNLMVERNDKKFTQVTMIETGDEFEVFFSQIAGEK
                     CTYVVGDDYFKAYGEDADENTNFWGVKPNY"
     gene            complement(11217..12221)
                     /locus_tag="PK345_00675"
     CDS             complement(11217..12221)
                     /locus_tag="PK345_00675"
                     /inference="COORDINATES: protein motif:HMM:NF013292.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoaspartyl peptidase/L-asparaginase"
                     /protein_id="HPX33567.1"
                     /translation="MKRRFLFLVPVAFILIVSCKNVVDEKQTVQDQCPEWVIVIHGGA
                     GYINQETFSAEKESQHKERMEAALLMGGEILKNGGSSLDAVEAAIRIMEDSPFFNAGR
                     GAVFNADGINEMDASIMDGKTGKAGAVANVTVIKNPISAARKVMEQTKHVMLIKQGAE
                     KFASEQGLDIVDPSYFFTQESWDEHINGLEKEDYGHKGTVGCVALDKNGNLAAGTSTG
                     GMTNKKFGRVGDSPIIGAGTYADNNTCAVSATGHGEFFIRNVVAYDIAARMKYRNENL
                     KDVADDIVNKQLKDIGANGGVIALDKNGNLAMPFNTSSMYRGYLKADETPKVFIFHDR
                     "
     gene            complement(12228..13010)
                     /locus_tag="PK345_00680"
     CDS             complement(12228..13010)
                     /locus_tag="PK345_00680"
                     /inference="COORDINATES: protein motif:HMM:NF014035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HPX33568.1"
                     /translation="MSWYEITVLFLTGLFAGFVNTLAGGGSIVSFSILLMFGLPPSIA
                     NGTNRINVLTQTFIASSSFKFQKVLDTKKALWLGVPALFGSVAGAWIAIDINEKVFER
                     VVGVILLVMLVFILYKPELWLKERKDLVNAKVTWKQILIFFLIGVYGGFIQIGVGFFL
                     MAGIVLSAGYELVKANAIKSFIIFLYTPVALIVFILNNQINWAYGLMIALGTIAGAYF
                     AARMAVKKGAKFLKWVIIVIILITSADLFGIIDIKGIVSLLN"
     gene            complement(13010..14928)
                     /gene="dxs"
                     /locus_tag="PK345_00685"
                     /pseudo
     CDS             complement(13010..14928)
                     /gene="dxs"
                     /locus_tag="PK345_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007173402.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
     gene            complement(15059..15373)
                     /locus_tag="PK345_00690"
     CDS             complement(15059..15373)
                     /locus_tag="PK345_00690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005468277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HPX33569.1"
                     /translation="MRDKSAIRHDIIEALKTLFDPEIPINIYDLGLIYEVIVSDDKDV
                     YVQMTLTSPNCPVAESLPMEVNEKILSVPGVKSVEVDLTFDPPWDEDKLSDEAKLELG
                     LL"
     gene            complement(15400..15822)
                     /locus_tag="PK345_00695"
     CDS             complement(15400..15822)
                     /locus_tag="PK345_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006797507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HPX33570.1"
                     /translation="MNIIEERGNQIVEEFSVFDDWLEKYNYLIEMGKDMPLIDSKYKI
                     KDNLITGCQSNVWLHPEFKNGKVYFYADSDAVITKGIINMLIRALSDATPDEIINADL
                     GFIDEIGLSEHLSPTRSNGLVSMIKQMKLYAMVFKTKT"
     gene            complement(15838..17058)
                     /locus_tag="PK345_00700"
     CDS             complement(15838..17058)
                     /locus_tag="PK345_00700"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HPX33571.1"
                     /translation="MNNEILKIREQFPILNRMVYGKPFAYLDNAATTQKPTKVVKRVS
                     DYYEWENSNVHRGAHFLSQEATVAFEKARTSVSQFINSSNSSEIIFTKGTTESINLVA
                     NSYGNKFLKKDDEILITRMEHHSNIVPWQMLCSRTGAKLKVSPIDFSGEMIMREFEKL
                     LNTKTKIVAITHVSNSLGTINPIKEVIEKAHDAGAVVLIDGAQAVSNLKVDVQELDCD
                     FYCFSGHKMYAPMGIGALYGKENLLDKMDPWQGGGEMIAQVTFDKTTYNEIPFKFEAG
                     TPNVSGALGFEAAIHFLSNIGYDFIVNHKDDLLNYGHNRLAEIAGLKFHGTAKRKTGL
                     ISFNIEGVHPYDAAVLLDKLGVAIRTGHLCAQPTVDFYNVPGFIRASFGVYNTREEID
                     LLCDAIEKVKEILS"
     gene            complement(17520..18164)
                     /locus_tag="PK345_00705"
     CDS             complement(17520..18164)
                     /locus_tag="PK345_00705"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HPX33572.1"
                     /translation="MIKIERLVFNPFQENTYLIYDESGECAIIDPGCSNVQENDELDK
                     FISDNSLKPVAHLYTHCHIDHVLGISHVNKQYGLLPLMHPESLKILQTVPSQGRMFGV
                     DIDQIVQPMNFINDGDTIKVGSFALEVLYTPGHVDGHVCFVNHFGRFVIVGDVLFLGS
                     IGRTDLLTGDFDLLEKSIKTKLYTLPQDYVVYPGHGPQTTIGHEMFNNPFVRGK"
     gene            18308..21721
                     /locus_tag="PK345_00710"
     CDS             18308..21721
                     /locus_tag="PK345_00710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33573.1"
                     /translation="MLPIYTRLLALPKPRLLIVGLLLFLLPQFGKGQFYEGYQMEFGR
                     SRVQYKDFFWTYYKFDRFDIYFYLNGKELAEHTAQYVTIELPQMENKLGTYISGKVQF
                     IIFNNLNELKQTNLGVNANIEYNTGGVSHILGNKVVLYFDGSIINFEKQIRQGIAHIL
                     LQNSIFGSNISSQMINSYLQTMPEWFTLGLISYLAEEWNTDIDNRIRNAIVSGKYKNF
                     NRLVSDELYVKDAGHSFWKFIADKYGNDNVINIVNMIRVSRNLNTGFQYVLGKTVKSL
                     YAEWFTYFNNIYTAEAKKFDPIPDDVRIKGRRILKRNNHQRFFSQLKSSSNGEHIAFV
                     TNETGKYHIWLYDYKTRKLKKLRTGGYLLDEKVDYSYPILSWHPTGRILSAIVEDKGL
                     NQLYFYDLVEKKWSQRNLFGFEKILDFSYSDNGQILLMSAVQQGQSDIFTFNLISGSY
                     ERLTNDFYDDLNPRFIENSTKIVFSSNRKSDTLIMGEKSDFETVSKNYNIFLYNYSTR
                     SQALRIITNIKDANAKQPFTYGKNYLTYLSDKNGIYNQYLGRIDSTVAYVDTAVHYRY
                     FTHSFPITNYSSSILSHDISPKGISNAFIINNKRFEQLFKKNLLLPEHLKPVQTTATY
                     YADQTISDTKSFGLLSGEIGEGIDNIEDTHDRPLLHRARKSFKNVMRDAASSADFDPE
                     YLIDTLLNDQVATFDPEFKMQGTIGINMPDSLNAMMDRFRKELKQSDSTKKFTFPIQR
                     NYYTEYSINQIVTQLDFSYLNQMYQPFSFTNSPYYSNPGFSPTFKIGITDLMEDYRII
                     GGMRLEFDLVNKEFFLHFANLKNRFDKEIIFQYRNLEEVIGSNYIIRQKIYEGYYIVT
                     WPFNRVLRLRTTLLARNENYIITGPYERLLKAPNVMFNWGGAKIQLIYDDSRELGLNL
                     PQGNKFMIFGEYNQKIDRSGNNLAVLGFDIRTYKRLHRSFIWANRIAGSTNFGSDRLI
                     YFMGGTDGWIAAKFDPTTRIDPDQKWTYQTLATNMRGFKQNARNGNNFIVLNSELRFP
                     LFTYLLNRPINSDILKIFQLVGFGDLGTAWSGWNPYDENNVLYTKYEYFGPLRIKVQY
                     EKDPIVGGVGFGARTKLLGYFIKGDLAWGIEDGKVKKTPMFYFSLSLDF"
     gene            22017..23918
                     /locus_tag="PK345_00715"
     CDS             22017..23918
                     /locus_tag="PK345_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HPX33574.1"
                     /translation="MSDSLHHECGIALLRLLKPLEFYQAKYGTPFYSLLKIHLMMQKQ
                     HNRGQDGAGLANLKLDIEPGVKYINRYRSNSPTPIIDIFNHIYRLFSKIQSSHPELLN
                     DVGWLKKNIDFTGELFLGHLRYGTYGGHNIENVHPVLRENNWMTRSLVLAGNFNMTNV
                     DELFNQLIELGQYPIETTDTITILEKIGHFLDEENEIHYQKYKKKGLVKKEITDKIAS
                     DLNIKKILSQAAKYWDGGYVIAGLLGHGDAFIMRDPSGIRPAFYYFDEEVAVAASERP
                     VIQTSFNVKAEQIQELKPGHVLIIKKNGRITEEQFTKPKQKQACSFERIYFSRGTDKD
                     IYKERKTLGKLLMPSILKSINYDLENTVFSYIPNTAAVAFKGLTQELNIFYNKLKTDQ
                     ILELGKDANAERINKILSSSLRIEDIAVKDIKLRTFITQENSRNDLAGHVYDVTYGII
                     KEGVDNLVVIDDSIVRGTTLRQSIIRILDRLGPKKIIIASSAPQIRYPDCYGIDMTKL
                     SEFVAFKAAIELLHDTRKEYIINEVYKNAKAQEQKPKEKIVNYVKNIYKPFTAQQISE
                     KISEIVTPHGINSKIEIIYQSIEDLHTAIPNNRGDWYFTGNYPTPGGNKVVNRSFINY
                     IENRNERAY"
     gene            complement(23944..26043)
                     /gene="recG"
                     /locus_tag="PK345_00720"
     CDS             complement(23944..26043)
                     /gene="recG"
                     /locus_tag="PK345_00720"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HPX33575.1"
                     /translation="MSYEFLKTPIEYLKGVGPKRAALLRSELNISTFDDLLNYFPFRY
                     VDRSTFSNINEIDNDQTYFQLKGKILQPQIVGAARAKRLTAYLKDDTGIIELVWFQGI
                     NWALEKIKADVEYIVFGKPNYFNGKFNLSHPEIEPVEDFDILSSQLRPFYPSTEKLKS
                     KNLNSRGIAKLTKLLISQIETLPETLSPQVINRFKFVSRHTAVINIHYPENKELLNKA
                     TTRLKFEELFFIQLALLRYKYVRTIRLKGHVFKLVGDNFNTFYKRYLPFELTNAQKRV
                     IKEIRADMGSGHQMNRLLQGDVGSGKTLVALMCMFIAIDNGFQASLMAPTEILAQQHF
                     KTISDLLLGMDLKAKLLTGSTRKAERQEIHDGLESGVVNIIIGTHALIEDTVQFKNLG
                     LVIIDEQHRFGVEQRARLWQKNEMPPHVLVMTATPIPRTLAMTVYGDLDYSVINELPP
                     GRKPVQTYHYYDNKRLMLYGFLKRKIAEGQQIYVVYPLIKESENLDLADLMAGYESLS
                     RTFPLPDYAISIVHGQMKTDEKDFEMQRFIKGETQIMVATTVIEVGVDIPNASVMVIE
                     NAERFGLSQLHQLRGRVGRGAQQSFCILMTSYKLTADGKKRIDTMVKTTDGFKIAEVD
                     MKLRGPGDMHGTRQSGMLDLRLADIIRDEKMLRYARDLAAEILKSDPDLVKNNNHILK
                     ERLSQLKKHQKDWSLIS"
     gene            complement(26136..27017)
                     /gene="gldN"
                     /locus_tag="PK345_00725"
     CDS             complement(26136..27017)
                     /gene="gldN"
                     /locus_tag="PK345_00725"
                     /inference="COORDINATES: protein motif:HMM:TIGR03523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldN"
                     /protein_id="HPX33576.1"
                     /translation="MKNHIVKNLLLGIFLLVLKVSFSQPGSQVQLVTEPRDRAYDRVH
                     INDLKPIPYSYLRESDVFWEKRVWTSIDFREKMNQFFYFPQTPQGKWRSFMQVVWDAM
                     LNGDITAYDATGSSDNFEEMLPVPVSQLQTNLTTQQTIRQEDPNNPGTFITQTVDIEF
                     DPQDVKFLWVKEDWIFDKQRSEMQVRIIGVCPVKEDIDPTTNLFRGYKPLFWLYFPEI
                     RPLLAQNEVFNSGNSARRLSYDQMFMQRRFSSYIIKEDNVFDRRIDSYTSGIDALLES
                     ERIKNEIRDFEQELWAY"
     gene            complement(27036..28643)
                     /gene="gldM"
                     /locus_tag="PK345_00730"
     CDS             complement(27036..28643)
                     /gene="gldM"
                     /locus_tag="PK345_00730"
                     /inference="COORDINATES: protein motif:HMM:TIGR03517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldM"
                     /protein_id="HPX33577.1"
                     /translation="MAGYKETPRQKMIAMMYLVLTALLALNVSKEVLDAFVVVNESVA
                     LTNENFSEKLNELYNTFDKQYQINQNKVKPFRDKAIEAKRLSTEMINYIDDVKWRLIE
                     VTERVPYDSAKLIPVKKLAKLDDFTTTTNFFMAGSTDGTKGEGIKLKNKIINYREKIL
                     SLVDEKNRGQIKIGLQTDGNYLDRDGNHLNWVQYNFYHTVLAANLAILNKMTTEVYNA
                     EFDVVNYLLASIDAADFKYDTVAARVLPKRDYVFIGDRYEAEILVAAYDTRQDPEVYI
                     LEGADRMTDEQINRARPIAGQKGVVKVNFPAQTEGLKRFAGIFRLTTGTGEVNSYPFS
                     ANYVVARPTTTISATKMNVFYAGVDNPISVSSPGVPLENLEVTISVGHIRKDNKPGNY
                     IVTVPVDKKEAIVTVSARIDGQLKKQGDNSYRIKRVPDPTALIAGYKEGAINRNVLAQ
                     VNAIEASMPQDFEFDLNFRITSFKMTTLSGSIVTDFKSNSNRLTDEMKNRIRNANRGD
                     RVWFEDIIARGPDGTDRPLNPISLTLN"
     gene            complement(28673..29740)
                     /gene="gldL"
                     /locus_tag="PK345_00735"
     CDS             complement(28673..29740)
                     /gene="gldL"
                     /locus_tag="PK345_00735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility protein GldL"
                     /protein_id="HPX33578.1"
                     /translation="MTTKVKSKESFSEKLETPFFRRFMSVAYGLGASVVILGALFKIN
                     HYAGANEMLLMGMMTEAIIFALSALQKPHVEPDWSKVYPELIEDYHGIKPDTPFERKS
                     ASVRTGSNQMTQLDLMLKDVKLDDSVIHSLGTGLTKLSESASKLNDLSNASIATNDFV
                     ANMKTASQSVSTLAKTYRDTSNALDNELKATNELTQQVQEVSHAASGLKNSYQEAART
                     LREDIRASEDLSLVIKTTSESVSKLSDSYYMSAEGISRTIEELRKSAGNSDAFNEQLA
                     TLSEKLASLNKIYELQLVNSERQVEASKRMQQTLDKFLSNLEESSNKTDQYQREMEML
                     TKRMASLNSVYGNMLSAMNIK"
     gene            complement(29815..31212)
                     /locus_tag="PK345_00740"
     CDS             complement(29815..31212)
                     /locus_tag="PK345_00740"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPX33579.1"
                     /translation="MHKIVNLTVKSAAHMKRLLFFFLALILISGCKNTGNGELVGVTK
                     RPSFSQPDPYGMAFIPLGSFVLGVGEEDIPYAQINNPKRVTVNSFYMDETEITNNEYR
                     QFVHWVRDSIARDLLGQVNPDAYLIEENKKTGEVYDPPFLNWGTDIKWDDEETAQTLE
                     EMYLPEQERFFRKKEIDTRKLKYKYFWIDLQSAARKDFTNKGDPEDASFANREIGPDG
                     RTKYVKEETINVYPDTLCWIHDFPYSYNEPLTKSYFWHPTYDNYPVVGVNWSQARAFC
                     VWRTALFNSYLQGKGRAGINDFRLPTEAEWEWAARGGFEANPFPWGGPYSRNDKGCLL
                     ANFKPNKGNYVDDGGATTVIVGHYQANSFGLFDMSGNVAEWTNSAFDESANFFTWDLN
                     PDYSYLAKPDDPISMKRKIVKGGSWKDIEYYLLVSTKDYEYQDTAKSYIGFRCVMSYL
                     GRSRGDNPRRASKVY"
     gene            complement(31323..32279)
                     /locus_tag="PK345_00745"
     CDS             complement(31323..32279)
                     /locus_tag="PK345_00745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPX33580.1"
                     /translation="MTSKFYTVLIFFYCFGIVGFSQQLPQYSQYMFNKIAYNPGVTGT
                     TNGICVGGLIRQQWVGFKETNAAGNSFNVAPETYVFTINAPVKALRGGLGGTIIQDKI
                     GHQDDISVNLTYGYQTMLGAGDLGIGLQLSIINKSLNFSNLEAPDGKGSDPVLQSEAE
                     ESDMYFDGGVGFYYSVPDNYYLGLSVLQLREAKKNDGTRMRLKRQINVIGGYEFTLPN
                     TPSIDILPSVMLKTDGTSAQYDFSALLRFNNKIWGGLTYRLQDAAAIILGIQFKELSI
                     GYSYDIPTSAIGSFGSHEVRVNYCFKVEIDKYKQSYRNTRFL"
     gene            complement(32450..33115)
                     /locus_tag="PK345_00750"
     CDS             complement(32450..33115)
                     /locus_tag="PK345_00750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33581.1"
                     /translation="MKRVILLILRLIYGRFSYVFQQYGIGLKTRNLTRQKYFLPCAGL
                     DALNHYKGAIQPSDKISVFNTDKRIDFQNNKFGITFRSLLRKNKRYSCYDITKYLDFY
                     WQRLGYRERVFNTGIRFIYHFIDKKFFFGEMFFSDATKVNVEQVAASLIKKYTNQMVS
                     TTETFKIMAKNGFIFFENTGINLSIKYIDTTNDFINKALETICDLNLSENVIIKNNLE
                     DLL"
     gene            33601..36510
                     /locus_tag="PK345_00755"
     CDS             33601..36510
                     /locus_tag="PK345_00755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33582.1"
                     /translation="MKTIIILKFSFREFKKSFLLFLFILTTVVAHCQQSGAGQQYQNQ
                     SYVTFPENVRAPYELPAIWSHQNKTENPHFIIVFLEANPRINDIPLEPGDFIGAFYTD
                     DYGNRKCGGADYWLGDENIIFPCYRDDDSTTEKDGFLPGEQIYFKLFCWATQKEYDVT
                     LVVFDPSFVTTDKWYPLGLSGIINIATFTVFDAYATATPNPICIGDEVTLSANILIGT
                     TGNYTYLWTSDPAGLNSTLQTVTQNPTVTTTYFLSVSDGTNISQHNIEVIVNTPPWIT
                     PGPDQTICEDQTVSVTAFPENNSGILWSSSGDGSFNNPSLINPVYTPGTADKANGNIT
                     LIATALPKAPCIEVATTDISVKINPIASLDLIENISFCKIDEMWISAEATNYSSIEWT
                     TNGDGTIINPNSTTIRYIPGSFDLSLGYFKLTCCVNSIAPCTQNVCKQTLVTIVTHPT
                     VNSPVNKKGCENTAISLNAIAKNYSSILWITNGDGVFNNPNILNPSYTPGVNDKLNGG
                     TVVTVTAFGNIGCEAFPASSNTTIIIESMPKLNPGNTGIFCKGYPLQMDASAQFYNSL
                     TWSTSGDGTFNNKNILNPIYYPSANDLETNSFDLTLTATAKSPCTGSVSLVLPITLVT
                     ETTALILTAAGLELCKGDTLQLNSEATGYTSIQWTTTGDGSFDDSTSLNPIYFPGPVI
                     DYSGIPIQLKLTAFTPQNCGPNIEKNIQVSFNLYEPEVFIGEDATITEPSTFFTNPVV
                     PNYTSLFWQTSGDGQFENPNNLITEYIPGILDLQNGSTLLILTTVGQGNCYGPALDTL
                     MLTIFRKHQIEMETGWQGISSFVTPLSSSFEILMTQISNQLAIAQNMLETYWPEYGIN
                     TINDFSNQNGYQVKLTSPTTLEVIGYDFKPKTVNFDFGWNIMPVASGCNILSSTITDQ
                     LGDKLIIIKEIGEQGVIWPQQGVYQIPYFIPGKAYMIKVTEPATVTFPTCTNPKK"
     gene            complement(36634..38703)
                     /locus_tag="PK345_00760"
     CDS             complement(36634..38703)
                     /locus_tag="PK345_00760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX33583.1"
                     /translation="MPVLMQGKEHAIGYHAYLKNQNITTCTLKLIRYLFLSVLIFVSL
                     FGKAQLNVDFFRKKGVDELNSKKFTEAIQTFNILVRGRPDLSEPYLLRGRAKLALGDL
                     KGAEFDFTRAIMNDNYNPEAYYYRGVVSSNLFDYFSALEDFSKSLERRPNNPNVLYSR
                     GITKIRMKNFEGAISDFDTLIIYRPDIEQAYLSRAIAKAENDDLQGAIQDCNKAIRLN
                     IYYVDAFVKRGLFYKMMGEYQEAIRDFDQAIKLEANNTLAYFYRAALHINLQDTMAAI
                     LDFDRVLALDPYNDLTYYNRGLIKASRKEFRDALTDMDMVLKINPKNIFTWYNRGIIN
                     LNLKEYNASIDDFSEAIKLFPDFAAAYMGRASAKQELKDYQGANEDYEYAIAIINLLN
                     DDDFSLLDQKYSADSDYLKRIIEFEADFNSYNILEGRIQYQHVLIELKPNVMIYYYPS
                     EKVILDEKKRGYVYEPLKEFKYNSDLFSLGINSEKVSLSVAEAKMISDQVDSIMYFNP
                     FDADNYFVKGVLNWMLMNYSEAYTSFSRAIELNPDYFEAYFSRATVLFELIELQYSFD
                     YTEPTIFLSADGSALSQNERENKIPDFASVISDYDKAIKLNPNMYFAYFNRANIKNRM
                     LDFEGAIKDYTIAVALNPEFAEAFYNRALTLIYLNRNAEACFDLSSAGELGIVEAYNV
                     IKRYCGK"
     gene            complement(38706..39467)
                     /gene="pssA"
                     /locus_tag="PK345_00765"
     CDS             complement(38706..39467)
                     /gene="pssA"
                     /locus_tag="PK345_00765"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003882 - CDP-diacylglycerol-serine
                     O-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HPX33584.1"
                     /translation="MIRKNIPNFVTLLNLLSGCVSIVFAFSGELRLASYLIGMAAIFD
                     FLDGTLARVLKVRSEIGLQLDSLADVISFGLAPGFIIFHLMNNSSNAPLILVYNINFA
                     LFVALLIPVFSAFRLAKFNIDNRQNENFIGMPTPTSAIFFASLPLVLHQASISNHTMV
                     KSLIENYWVLSLLVIVFSYLMVSSVVLMSLKFKTLSFTANSTRYIFLFVALVLVLFLK
                     FYAFPIVILFYILLSVLFNPAKRSIISKRQGANHS"
     gene            39572..39823
                     /locus_tag="PK345_00770"
     CDS             39572..39823
                     /locus_tag="PK345_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33585.1"
                     /translation="MSPEEFMRLPAVERVTLILNDGRELMDRIYLYYLIRLYKIFDFY
                     IELWYLPASNKIDKISIVELDDVMHLYEKNIDISDIFNT"
     gene            39832..41058
                     /locus_tag="PK345_00775"
     CDS             39832..41058
                     /locus_tag="PK345_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HPX33586.1"
                     /translation="MDRLVQLKIEDFWYELPENKIAKFPLQTRDESKLLVFNGIDIQV
                     DHFKNLATYLPNNSTLVFNNTKVVNARLFFRKETGALIEIFCLEPSTLFPDIQIAFQQ
                     KGTALWKCLLGNARRWKSGVLSGKFNHGNTTVIIKAEKISQLADGFEIRFTWEPADLT
                     FSELLQYAGEVPLPPYLNREPVESDKYQYQTVYAEKYGSVAAPTAGLHFTDRVLESLS
                     AKSIKPANLTLHVGAGTFKPVGTEGIKAHIMHSEQITVTRKLIEDLLENIGNIIAVGT
                     TSVRTLESLYWFGAKLENDNNASFNIHQYDPFHLETKNDTSVEAALINVLDYLKKNQL
                     ESVNGSTRLMIAPGYTFKLICGMITNFHQPRSTLLLLIAAWLGDYWKEIYEYALNNDF
                     RFLSYGDSCLFIRQKI"
BASE COUNT        13212 a         8289 c         7088 g        12959 t
ORIGIN      
        1 aaaaaagacc ttcagcgtat cttcagagat tattaattgg ttcaactcaa aattgaaata
       61 ttatcagatt aggccaatat gacttcctta aaaaatgcta ctatcctctt acttatatcc
      121 tttcgttatt atcccgctaa aatagataat ctttacagaa ataattcaat caattaataa
      181 tatcaaaaac aaaaaaggct gcctcatttt tgaaacagcc tcttttaaat attctgaaaa
      241 attattttac aataagttta tgtgatactt tttcagcgcc atttgaaact gtgtagaaat
      301 aaattccgga tgttaatgct gaaacatcga aagatatagt attaaacccg gcattcattg
      361 atttaacagg gagttcaaat actttttggc cagtcatatt aaatatttca aatccaatat
      421 ttgcagcttt gtcaagttca acaccaacaa ttgtcgaact ggttgcagga tttggatagg
      481 cttcggtaac tttgaatggt gaaaccatgt tcgcaggttt ttcaatccca acggtaaagt
      541 tcatattgtt atggattacc ctgttgacaa cagcgtcatg gtcttcgtca agatataatc
      601 ctggaaggtt gtcagcttga tagataagct ggaaagctcc tgaaccatta gttttatcag
      661 caagaatagg ataaatacat tcatcataga gatggaaaat attatcacct tgtaagtcgt
      721 agaaatcacc ccatgtttct ccaaggtcat aggaaaatct tgtccagata tgcttatagt
      781 tcattgtacc atcagcagta acataggttt ctgttactga cgaataggca accataatca
      841 tgctaccttc aattgcgatt gttggcattg tagaaattcc gagggaacga tatgcattaa
      901 atggcgtatc gttagtacct tcaaaatcaa atataaaacc actattattt acgtcctgtg
      961 tccaacctac taacatgccc atttcctcaa gatattctgg catcattgta cggtgtggat
     1021 tatcagcttc tggaatttgc cccatgcttt cgttccagta tccaatacca tcggtatatg
     1081 gccagtagct gtaggaaccc ggatcgggtg gtgcagattc taaacgagcc acgcgagaga
     1141 taccccatac tacatgcacc attccattat catcaatagc aagactagct gattgatcaa
     1201 ctgaatataa agtatcgctt gttaaagtag attgtacatc aaagaatgga tatgggtgtt
     1261 cccaaataat gattttctcc caggactcac cattgtctgt tgatttaagt aaaaacaaat
     1321 ctttccaagc acttgattgc aacaatgcca cagtatttcc tttaaaagcc atggtgtaat
     1381 catcagctct gatattcaga taataatcag agcccacgcc atcaattagc tgatgctgaa
     1441 tatcccatga ttctccacca tctgttgagc ggttgtagag taatgctcca tcctgtccca
     1501 tatatggagt tccaccattt cctgttggag ctgtgagggc aaaaagatgg atatactcat
     1561 tattttgtcc ggagctgatc atacgtggcc acaaatattg tggagcagct gtaccattgg
     1621 ggccattata tgtactttct atccatgccc cagtaccttt tgtctctctt ttcaagagaa
     1681 gtaaaggatt acctcctccc tggtgtgata cgacaaactc tccgttagcg ccccattttg
     1741 taatggaagg ccagccggct ttcttagact caattctcgt tgatggttta ggcccccatg
     1801 aagagccatc aaaatagttg tagcctgtac ccctgtcagg gaaagcaaaa ccaccaggac
     1861 tttcgacacc acgcgtacaa actgccgcca tagtcccatc atcccaaaca acaaacctgt
     1921 tagcaacagt agagttagtc tgaagatcat agatggtctc aatgatttcg gcttcatcac
     1981 ccaacatctt tgcttctttc ataagccccg gataagtata attgaaatta accataggag
     2041 cctcaagatc agaaggcatt tcatacactc ttgttaacga catttttgtt aattcttttg
     2101 atataggtac ccttttttgc gaataagcaa tggttaccaa acaaaaagaa agaactacaa
     2161 gtagaacctt tttcataaaa ttaagttttt aatgggttaa taaattcttt tacgggtgca
     2221 aaaataaaaa tatcaagtta attgacacaa ttaatgaaac aaaaaattta actctaaaaa
     2281 aaaaggttat aaaatcttga taatatcaaa aaaaagcgct gctccatctt ttgtagtcct
     2341 cacagcagca actattgaaa tgtacacact tatgttattt aaagaaatat ctttttataa
     2401 acaaaaattt aaggatccgg cgttactctc ttttttataa gctatctaat tgggtacaat
     2461 taatttacat ttaggaaaga ttagcatcag attcggttag atttgtcttt gaaatttgca
     2521 ttttttagct actcgctaat tgaatttaaa aactataatt ttttattttg taaaagcaaa
     2581 catagcctac ataaccttac ttttttaaaa aaaaacgata aaatgcttga cttcgccttt
     2641 ctattcatct atatttgcag tcatttacaa aaacatattc ttttctaact tcataaaatt
     2701 atgaaattat cacagtttaa gtaccattta ccatccgaac tgattgcttc tcagccacct
     2761 gaaaatcggg atcaatgcag aatgatgatt ctcgaccgta aaacaaaatc tattgagcat
     2821 aagaattttt ctgacattct tgattattat gatgacgggg atgtttttgt actcaataat
     2881 acaaaagttt ttcctgcaaa gcttatcggc atcaaagaaa aaaccggcgc acgcatcgaa
     2941 gtttttctac ttagagaact taatatcgaa acgcggttat gggatgtttt agttgatcct
     3001 gcaagaaaaa tcagaattgg caacaagctc tattttggcg aacaagacga gctggtagct
     3061 gaagtgattg ataacaccac ttcacgtggc cgaattttgc gatttcttta cgacggcccc
     3121 tatcctgaat ttagaagaat gttgcaagct atgggtaaaa cgccactacc taaagctata
     3181 aacagggagg taaaacctga agatgaagtc tggtatcaaa cgatctttgc caaacacgaa
     3241 ggagcagtag tagctccaac ggcaggtctc catttcaccc gtgagcttat gaaacgattc
     3301 gaaataaaag gaataaattt tgccgaatta acactccatg ccggtttggg aaatttccgc
     3361 caaattgaag tagaagacct ttcgaaacat aaaatggact cggaagatat gaatgtggat
     3421 gaaaaatgtg ttgaaatagt aaacaaggca aaagatgata aaaaacaaat ttgttcggta
     3481 ggaacaacca ccataaaagc aatagaatct gccctgacca tttcaggaca tttgaagccc
     3541 tatagtggtt ggaccaacaa atttatctat ccgccttatg atttcaaact tgcaacctca
     3601 atggtaacaa attttcatct cccaatgtcg aatatgttga ttcttacagc tgcatttgca
     3661 gattacaatt ttttgatgaa agcatataag gaggcggttc aaaagaaata taagtttttt
     3721 tcttatggtg atgctatgct gatattataa agattaaaag ccttttccat taataaaaaa
     3781 aacgctattc gaatgaataa aaaatagttt tggaaataag tgtgtctctt ttttgttttc
     3841 aagtttacta aaaatgtctt taaaataaga aaaacagcac cctgcaagta tgaataaaac
     3901 tgtgattatt gttgctggcg gatcgggcaa gagaatgaat tacggcattc ccaagcagtt
     3961 tattacccta tcaggactgc ctatactaat gcatacaatc tctcggtttt atcattcatg
     4021 caaggacatt aacatcattg ttgtattacc tcccgaccat attgaaacat ggagccggct
     4081 ttgttctgaa catagttttt ttatatcgca tagagttgta aaaggtggaa gccagcgttt
     4141 ttattcagta acaaatggcc tcgaatatat tggcacaggt gagttagttg ctattcacga
     4201 tggcgtaagg ccattaatta ctaaagaact tataatgcat ggctacgaaa ccgcctctac
     4261 atttggaagc tctatccctg taatacctct tcctgggtca cttcgtaaaa ttgagggcaa
     4321 ccatagttat tccattgatc gtaaccacta ttgtttggtt cagactccac agacttttca
     4381 atctgagctt attatcaaag cctatagtca gccatataga aaagagttta ctgatgacgc
     4441 ttctgtttat gaagctgccg gctttcaagt tcaccttatt gatggaatat ctgaaaacat
     4501 caaaatcaca ctttctatgg atttaaaaat tgcgaatgtg cttatcaaag atttttcaca
     4561 ataaacacat tttaaaatga ttttttacat aggctgctta tttttgcata aaacctaatt
     4621 cggataatgg aaaatagacc agagcgcata atacttggca ttgatcccgg aaccaccatt
     4681 atgggatatg gagtaataag cgacaatgga aagtcagtta cgatgttggc aatgggtgta
     4741 atcaaactat caaaatttaa tagccaggca ctgaagctgc gaaggatatt tgaacgtaca
     4801 cttggtttag ttgaacaata taagcctgat gaactttcga ttgaatcgcc cttttatgga
     4861 aaaaatgttc agtcaatgct taagctcggc cgtgcgcaag gtgttgctat ggcggcggct
     4921 ctatatcgcg acatcccaat atttgaatat tcacccttgg aaattaaaaa atcaataacc
     4981 ggaaatggta gcgcctccaa agaacaagta gccgctatgt taaatcgttt attaacattt
     5041 gataaaaacc cggaaacatt ggacgccacc gatgggttag ctgcggcaat gtgtcattac
     5101 tttaaacgaa caaaaggtat caagggaaaa acttatgcag gatggaaaaa ctttctggct
     5161 gataaccctg ataggatcaa gaaataacaa aagaataaaa tatctattag gtaactaaga
     5221 acaaaaccga atgttacgct tttagttttt cggtcagcca ctctctggca ttcacaaaag
     5281 cttcaaacca tggagacatc tgatcagttt ttcttttttc aggataatag ggccactgcc
     5341 aggggaaaat aacacgctca atgtgtggca tcatagccaa atgtcggcca tcatatgaga
     5401 caatacctgc cgcaccaaat ggcgaaccat taggattacc cggatattcc tgatacgaat
     5461 atttgattgg gatgcaataa ttagactcgt cctgcggaaa atgaaatctc ccctctccgt
     5521 tagctaacca aataccaagg cgactacctg tcaatgattt caacatcacc gaattattag
     5581 gtaaaatatc aacagtgaga aaagccgatt cgaacttgcc tgaatcattg tttttcattt
     5641 taggatgctc tttatgatcg ggatagataa ggcctaactc ggtcattacc tgacagccat
     5701 tacaaatacc aaggcttaat gtgtcggctc tactataaaa attacgaatt gcagtgttgg
     5761 ctttatcatt gtataataat gcgccagccc atcctttacc tgatccaaac acatcagaat
     5821 tggaaaagcc ccccacaaaa acaatgaaat ttatttcttc taatccctct gtacctttga
     5881 taagatcggt ggtatggata tccttcacct caaagccggc aaggtgaagc gcccatgcca
     5941 tctcacgatc gccgtttacc cctttttcac gaataatcgc agctttaatc ttcttcccgc
     6001 ccatctcttt aggttttatt ccgagttgag aaaatttccc taaaaaaata tccgaaaagt
     6061 taaaaattaa tggctgcttc ttgtaatttc tgtaacgagc caaggcaaac ataggcttag
     6121 tttgattacg atcgaaaaga taagaggtct taaaccagat atccctcaac gagtcaatat
     6181 caaaatgatg aacggtaccc caattttgaa ttctaacctc tcgtaacgaa acatacttac
     6241 cgattttaaa agctactata ccggaagatt tgagtaacga caagacttct tgatcttttt
     6301 taacctgtat aatcacagcg ggtttttcgc taaaaagtaa tcgtatcaag tccttttcac
     6361 ataattcagt aagatctata tccagtccgc ctttcaggtt tgagaaattc atttcgagga
     6421 gggcagtaat caatccacct gacgaaatat catgtccggc aataattaag ccttcattaa
     6481 gcaagttttg aatggcttca aaactcttct taaaatattc aggatcagaa atatctggag
     6541 gggtcatccc aagcattcca aggatttggg caaaactgct accgcctaat tcaaatcccg
     6601 atttagtaaa tggcacatag ataatttccg tattttcatc attagacaat actggtttta
     6661 caattttttt aacatcagag acttctgcaa cagcagatat caccactgtg cccggagcaa
     6721 gcaccttttg tccgtccgga tatttttgtg tcatcgaaag tgaatccttt cctgttggaa
     6781 tgtttattcc cagcgaaata gcaaatttac ttgcagcttc aaccgcagaa taaagacgtg
     6841 cgttttctcc ttcgttacga gcaggccaca tccagtttgc agataatgaa acacctttca
     6901 aaccgtacct aagaggagcc cagacaatgt tagtcaaagc ttctgaaata gcaagaaccg
     6961 atcctttagc cgcatcaatc aaggcggcaa ccggtgcatg gccaatggaa gtagtaatgc
     7021 cattaacacc ctgataatca atagcaacag cgccaagatt attcaatggt aattgtagtt
     7081 cgccggcgca ttgttgtaaa gctatttttc cggttaccga acggtcaact ttatttgtga
     7141 gccaatcttt acatgctaca gattctaatt gaagcacatt ttcaagatag tcctgaattt
     7201 caagaatatc atattgaagt tcaacaaatt gatcgttaat aatgttatcc gttaaaatag
     7261 tttttggcgt ttttccaaat agatccgaga gagatagatc cattgcatgt atttgagaat
     7321 tctccctttg aaatgtaaac cgtaagtcgc cggtaatctt tccgacttga taaaacggag
     7381 ctctttcgcg ttcggcaatt tgttttaaca atgacacatc tctatctttt atgaccagcc
     7441 ccattcgttc ttgcgattca ttaccgataa tctctttatc cgataatgta ggatcaccga
     7501 ttggtagttt ttcaattctt attgcagcac cggtctcttc tgccaattca gaaaggcagt
     7561 ttaaatgtcc gccagcaccg tgatcatgta tggctataat aggattttga tctgactctg
     7621 ccatagctct gatcacgttg taaactctct tctgcatctc cggatttgag cgttgtactg
     7681 cattcaattc gatttgatga tcaaactgac ctgttgccac cgatgaaacc gcaccgcctc
     7741 ccattccaat cctatagtta tcaccaccca taacaatcac taaatcatca ggttgaggct
     7801 tctctttatg agcatcaatt ctttttccaa agcctatacc acccgctaac attacgacct
     7861 tatcataacc ccattttttg tcatcactat taaattcaaa cgttaacaaa cttccacaga
     7921 taagcggttg tccgaattta ttaccataat cgcttgcacc gtttgaagct ttgattaaaa
     7981 tatccaatgg cgtgtgataa agccactgac gctcaacaaa ttgttcttcc cattggcgag
     8041 ttgcttcaag ccttggatag gaagtcatat atactgctgt gcctgcaagg ggcatactac
     8101 cctttccacc cgccattcgg tcgcgtattt caccgcctga accggttgca gctccattaa
     8161 aaggttcaac ggtagtaggg aaattatggg tttcggcttt taacgaaata acggtatcaa
     8221 taactttcgt tttaaaaaaa tcaggcacat cttgccttac aggagcaaac tgctcagcct
     8281 gagggccttc tatgaaagcc acattatctt tatatgcaga aacaatatga ttgggatttc
     8341 tcctggaagt ctctttaatc aacgaaaaaa gagagtgcgg catctcttta ccatcaataa
     8401 taaaggtacc attaaaaatt ttatgccggc aatgctccga gttaatctgt gcaaagccgt
     8461 aaacttctcc atcagtaagt tttcgtccta ttctttcact aagttcatca agatatttta
     8521 cctcatcctt gcttagcgcc agtccttcag tttcgttata aagcgctata tcttcgataa
     8581 agagaatagg attcggcagc ttatcaatag tgaataaccg ctgatcaagt ccggtataaa
     8641 gcgatgataa caaagggtcg aaggcaatat ttttgttttc cacttttttg aactcttcca
     8701 tccggccaat ccctttaatc cccatgtttt gagttatctc tacagcattt gtactccatg
     8761 gtgtaaccat ctctttacgt ggtccgataa aatatccatc aattttatct gaaattatct
     8821 gttcagcacc attcaaaagc cacttcagcc tgataagttc attatcggta agcggattgt
     8881 ttttcgataa tgcaataacc gatgattcgt tagaaaagaa acagatcata aatacgttgt
     8941 atatttatta aatagagaca aaagtaggaa atgaatttag atttaatgtt ttaaacgatt
     9001 aatatctttt tttaattcct gtaagctcaa aaataggggt gttacgtaaa taattaaaaa
     9061 gcggaattat gagtcaaaat gctgaacgtt ttctggcctg ctttaacgct atagagaggc
     9121 atcttcaaaa gaactacaat aatggcaatc atgggccatt caggatgtta ctaaatactg
     9181 ctgccaacaa aaacgctatt tataaacggt tcttagatga tctttatgct atcggagatt
     9241 tacgtaatgc tattgttcat agcgatcggt ttgatggccg acctattgcc gaaccagtgg
     9301 atgaaattgt tgagaaagtg gaagagatat ggaacatcat tcagcatccc gacaaagtaa
     9361 acatctttga aaaaaaagta ctgtattgtt ttgtagatga ttatcttagc aaagcattaa
     9421 atataatgct aaagcacaaa atcaccttta ttcctatttt gcaaaataac ttaattattg
     9481 atgtacttaa tggcaaccac atcacctatt ggcttgccaa ccaaacgact gtctccacca
     9541 ccgaaaccac tatccgcgaa gtgctgctcc atgccgaata tcgtgggaat tttgcactaa
     9601 tatcacgcaa agaatcagta tatgaagcca ttgagatttt taagaaatca taccgcgaag
     9661 agcctaaaaa cagatattat gatgcgatta tcattacaaa ccatggtagg aaagatgaaa
     9721 aaatgaccgg tataatcgtt ttaaaagata tagttactta tctggctgat aaagagatat
     9781 aaaagtatct gaaggtcctt ttagtttatc ttcaccgttc aattttattt tttatccaca
     9841 atagttgaat tagcctgagc cagtggtgtc atcaaaatat catttacgca aacgtgatca
     9901 ggcagtgtag ttataaaaaa ggctatctca gcaatatctt ttccctctaa tggcttatat
     9961 ccttgatata cttttgaggc ccgctcttca tcacctttga atcggactaa agaaaactcc
    10021 gtttcaacca atccgggggc tatttgagta acttttatcc cattgccaag caggtcaata
    10081 cgcattgact tagttaaagc gtccacagca tgtttagtgg cacaatagac gttgcctttt
    10141 ggataaacct ctttaccagc tatggatcca atattaataa tatggccttt ttgccgggca
    10201 atcattaaag gcaatacagc tttagaaaca taaagaagcc ctttcaggtt agtgtcaatc
    10261 atctgttccc aatcatcggg attaccatgt tgtatgggat caaggcctac ggcaagtccg
    10321 gcattattca caagtatatc aattgatttc cattcatcag gcaaaccatt aatttctctc
    10381 tctactgact cacgatcagt aacatccatt ttaagtgcga gtatatttgc ctttgtattg
    10441 gaagtaatat ttgacttcaa agcgttaagc ttatcgagcc tacgtccagt gataattaca
    10501 ttaatatctt tttgggcaaa ttttaaagca atagcctcgc caattccaga ggtagcgccg
    10561 gtaattaaag caatcttcat atagtacagg atttatctac atttttaaat cataactacc
    10621 aaaaaacacc taaaaaacct gtaaataatc aataatttgg ttttacgccc caaaaattag
    10681 tgttctcatc ggcatcttcg ccataagctt taaaataatc atcgcccaca acgtaagtac
    10741 atttttcacc ggcaatttgc gaaaagaaaa cttcaaattc gtcccctgtc tctatcatag
    10801 ttacctgtgt aaatttttta tcatttcgct ctaccatcag gtttttatgg cacaggggac
    10861 agtaaaaatc aatatgctca ccggcgatta ttttaaatgt aggatgctta atagctcggt
    10921 aatctcccaa ttcgggtgtt aataaaatca accctcgggt accatcatgt ttttgtgcgg
    10981 agaggactat ccgttcgttc agataaagtt gcccggaaca gaaaggacaa atgaaatttt
    11041 tttgcatgat tttaatggat tattataaaa caaaagtcat tttttcaaga caaatttaca
    11101 aattttcaat atttgagttt gattgatttt acaaatatga tgttttcaat ctgcatacta
    11161 tttaattaca aaacaccatc atcattcata tatttcgagc aatacccaaa aaaattttat
    11221 ctatcgtgga aaataaaaac ttttggcgtt tcatcagctt tcaaatagcc tcgatacata
    11281 cttgatgtat taaaaggcat agccaaattt ccatttttat ccaaagctat taccccacca
    11341 tttgcaccaa tatccttcaa ttgtttgttt acaatgtcat cagcaacatc tttgaggttt
    11401 tcgtttcgat atttcattct tgcagcgata tcgtaagcga ccacgttacg gataaaaaat
    11461 tctccatgcc ctgtagcaga aactgcacac gtattattat cggcataagt tcctgctccg
    11521 ataatcggcg aatcgcccac acgtccaaac tttttattag tcatcccgcc ggttgaagtg
    11581 ccggctgcca gatttccatt tttatctaaa gctacacatc caaccgttcc tttatgtcca
    11641 taatcttctt tttcaaggcc gtttatatgc tcatcccagc tctcttgagt gaaaaagtat
    11701 gatggatcaa caatatccaa tccctgttcg gaagcaaatt tttcggctcc ttgtttaatc
    11761 aacataacgt gtttagtctg ttccataact tttcgggctg ctgaaatagg attttttatt
    11821 acggtaacat ttgcaacggc tccagcttta ccggtttttc catccattat tgaagcatcc
    11881 atttcgttaa ttccatcagc gttaaacacg gcgccacgac cagcattaaa aaatggcgaa
    11941 tcctccatta tcctgatagc tgcttcaact gcatcgaggc tggaacctcc atttttaaga
    12001 atttcaccgc ccatcaacag agctgcttcc atccgttcct tatgctgaga ctctttttca
    12061 gcagagaaag tttcctgatt tatataaccg gcgcctccat gaataactat tacccattca
    12121 ggacattgat cctgaacagt ttgtttttcg tcaactacgt ttttacatga tacaatcagt
    12181 ataaaagcta ctggtacgag aaataaaaat cttcttttca taatttatca attgagtaat
    12241 gatacaattc ctttaatatc aataatacca aataggtcgg cggaagtaat caggattatg
    12301 acaataataa cccattttaa aaacttagca ccctttttaa cagccatacg tgcagcaaaa
    12361 taagcccctg caatagttcc taaagctatc atcagaccgt acgcccaatt aatctgatta
    12421 tttaaaataa atacaataag agcgacaggg gtataaagaa aaattataaa cgacttaata
    12481 gcattggctt taacaagttc gtaaccagcg ctaagaacaa ttcctgccat cagaaaaaaa
    12541 ccaaccccta tctgaataaa accgccataa acaccgataa gaaaaaatat taagatctgc
    12601 ttccaagtta cttttgcgtt tacaaggtct tttctctctt tcagccacag ctcaggcttg
    12661 taaagaataa aaaccaacat caccaacaat atcactccga caacacgttc aaacactttt
    12721 tcattaatat caatagctat ccaggcgcca gccaccgagc caaataatgc cggaacacct
    12781 aaccaaagcg ctttttttgt gtctaagact ttttgaaact taaagcttga actggctata
    12841 aaagtttgcg tcagtacatt gattcgatta gtgccattag caatggaagg gggtaatcca
    12901 aacattaata gaattgagaa agaaacaatg gaaccaccac cagcgagggt attgacaaat
    12961 ccggcaaaaa gccctgttaa gaaaagtact gttatctcat accagctcat tacgatgctt
    13021 ttattttttt gattaatcga agtactatat tttcgattcc aacagcatca tatccacagt
    13081 cattccacaa ctgccgttgt gtgccatgtt ctataaactt atcgggaata ccgagtgaaa
    13141 caacagttga attaaaaccc tctttattca taagttcaat cactgccgat cccaatcctc
    13201 cttttatcgt accatcttct actgtaacga tattgctata atccctgaaa atattgatta
    13261 aaaggtcgtt atctaaaggt ttcagaaatc tgatatccac atgtgtaatt gttatctctt
    13321 ttttctctaa ttcgtggcaa gctttgatta cttcatttcc gatatggcca atagagataa
    13381 ttgcaacatc ttttccttgt cgtacaattc gacctttacc aacttcaatt tcagaaaacg
    13441 gcaattgcca atttgtcatc acaccttttc ctcttgggta tctaataaca aaagggcctt
    13501 tgtcatttag ttgagctgtg tacatcatgt cacgcaattc ctgttcgttc attggggaac
    13561 aaattgttaa ttcaggaatt gctcttaaat aagccaggtc gaaagcgcca tgatgtgttg
    13621 ggccgtcctc acctactaat ccggcacgat caaggcaaaa gacaacattt aacttttgga
    13681 gcgcaacatc atgaatcaat tgatcgtaag ctcgctgcat aaaagtagag tagatattgc
    13741 agaaaggtag aaaaccttct gtagctaaac cagcagcgaa ggttacggca tgttgctcgg
    13801 caattcctac atcaaaaacg cggtgaggca tttgtttcat catcaaatcg agtgagcagc
    13861 cggttggcat tgccggagta atacctataa tctttgggtt ttgtaatgcc agttcaatta
    13921 tcgttttccc aaatacaatc tgatagttag gtggtacctg ttgatgatca acatcgttat
    13981 taataagttc tccggttttt ttatcgaaag tagaaggtgg cgcatgataa ataacgggtt
    14041 gtttttcggc catctccaat cctttacctt tgatggttac gatatgtaat agttttgggc
    14101 ctgtaatatc tttaatgtca cggagtaact tggtcaattt caacacatca tttccatcaa
    14161 cagggccaaa atatctgaaa ttaaaagcct caaagaggtt actcttggta aggatactgc
    14221 cttttacagc attgccaacc tgtttgacaa tggctcgggt gtttgcacca tatttagttc
    14281 ctccacccag aagatgccaa attttatctt taaagcgatt gtagattttt gaagttgcaa
    14341 tatttgcaag atactctttt aaagctccta catttttatc tattgcaata ccgttgtcat
    14401 tcaagataat aagtaagtta ggattgttta caccagcgtt gttaagcgct tctattgcca
    14461 ttccgccagt tatagcacca tctcctatca cagcaatatg atgccgatta tagaggcctt
    14521 tcatttttgc agctacggcc attcctaatg ccgccgaaat agatgtagag gaatgaccta
    14581 ctccaaaagc gtcatattca ctttccgaca ttttaggaaa accgctgata ccatgctgtt
    14641 tgcgattagt atgaaaaaca tcgcgcctgc ctgttaatat tttatgccca taggcctgat
    14701 gaccaacatc ccataccaac ttgtcgtcag gtgtattaaa aacataatgt aatgctacgg
    14761 taagctcaac aacgccaagg ctcgctccga gatgagcagg attgacagaa acggcataat
    14821 aaattccctc aactcatcgc aaacatcagg aagtaacgac tcatcaattt ttttaagatc
    14881 agcaggatta ttgatttcag acaaatattt ccacgattgc ctgtccatat ttttttttag
    14941 aaaacttttg ttaaaccgtt attaaaaaat catcgaaaat ataatgacct aattttaaag
    15001 ctttattaat aagcatagag ttgaattact tttttcatta gtcaatatca atatttagtt
    15061 acagaaggcc aagttcaagt ttggcttcat ccgagagttt atcttcatcc catggtgggt
    15121 cgaaagtaag atcaacctca acgcttttaa ctccaggtac tgaaagtatt ttttcgttta
    15181 cttccatagg cagactctca gctaccggac aattgggcga agtaagcgtc atttgaacat
    15241 aaacatcttt atcgtcggag acaataactt cgtaaattaa acccagatca taaatattaa
    15301 tcggtatctc agggtcaaat agagttttta atgcttcaat tatgtcgtgc cttatggcag
    15361 atttatctct cattgtatca ctttttagga ttgttttgtt taagttttgg ttttaaatac
    15421 cattgcgtac aatttcattt gtttaatcat cgaaacaagg ccatttgatc gcgtgggcga
    15481 aagatgctca ctcaatccga tttcatcaat aaaacccaga tcagcattga ttatctcatc
    15541 aggggtagca tccgaaagcg ctcgaataag catatttata attcctttgg tgatcaccgc
    15601 atcgctatca gcataaaaat acactttacc attcttaaat tcaggatgaa gccatacatt
    15661 tgattgacaa ccggtgataa gattatcctt aattttatat ttcgaatcaa tcaatggcat
    15721 atccttaccc atctcgatca agtaattata tttctcgagc caatcgtcaa aaacgctgaa
    15781 ctcttcaact atctgattcc ctctttcttc aattatattc attttttaaa cttttcatta
    15841 ggatagtatt tcttttacct tttcaattgc gtcgcagagc aaatcaatct cttctcgggt
    15901 attatatacg ccaaatgagg ctctgataaa acctggaaca ttataaaaat caacagttgg
    15961 ctgtgcgcat agatgtcctg tacgaatagc cacacccaat ttatccaata atacagctgc
    16021 atcataagga tgaacaccct cgatattaaa tgaaattaat ccagtttttc ttttagctgt
    16081 tccatgaaac ttaagacctg caatttcagc aagcctatta tgtccataat ttagcaggtc
    16141 gtctttatgg ttaactataa aatcatagcc aatattactg agaaaatgta ttgcagcttc
    16201 aaaacccaac gctccggaga catttggagt gcctgcttca aatttaaaag gtatttcatt
    16261 ataggttgtc ttgtcgaaag taacttgcgc aatcatctcg ccaccgccct gccacggatc
    16321 cattttatca agcaggtttt ctttaccata aagagcgcct atgcccatgg gagcatacat
    16381 cttatgtcct gaaaaacaat aaaaatcaca atccaactct tgcacatcta cttttagatt
    16441 cgacaccgcc tgcgctccgt caataagaac tactgcgcca gcatcatggg ctttttctat
    16501 aacctctttt ataggattaa tagtgccaag cgaatttgaa acatgagtaa tggcaactat
    16561 cttggttttg gtattaagca atttttcaaa ctccctcatg atcatctcac cagaaaaatc
    16621 aataggagat actttaagct tagccccagt ccgactgcac aacatctgcc aaggaacaat
    16681 attactatga tgttccatcc ttgttatgag tatctcatca tcttttttta gaaatttatt
    16741 gccataagaa ttggctacca agttgatgga ttcagtggtt cctttggtaa aaataatttc
    16801 cgatgaattt gaactattga taaactgcga cacgctggta cgtgctttct caaaagcaac
    16861 agtagcttcc tggcttaaaa aatgcgcacc acgatgaacg ttactgtttt cccattcata
    16921 ataatcgcta actcgtttaa cgacctttgt cggtttttga gttgtggcag cattgtcaag
    16981 ataagcgaat ggcttaccat aaaccatacg atttaaaatt gggaactgct ctcttatttt
    17041 taatatttcg ttattcacta aaattaatca ttagaaaagg gctgcaaatt agtaaataat
    17101 taagaaatga tttatgaata tcataagtac gaagccttgt ccttaattag tatgcaaggt
    17161 gccaaggcat taaaaagaga taccactcct gatgttacat caaaatgatc aagaaaatgt
    17221 tttttttatc tccatcgtgg cgcggataac acttttggca aaataattct tatcagttat
    17281 taaaaaaggg ataacgaaat ttattgatta gagacactgg ttccgtgtta gtctggttac
    17341 gactcatgag gctacaacat cagcgctcca tcagtatcct gtactataga cgacaacttc
    17401 tcctttttgg taatgtaacc taccatttca aaattatttt ctttactatg gttttttggt
    17461 tatttgtttt cttaataatt aattttttgt aaaaatcatt tgtattttat cgctgtggct
    17521 tattttcctc taacaaaagg attattaaac atctcatgcc cgatagtagt ttgaggtcca
    17581 tgtcccggat aaactacata atcttgtgga agtgtgtaca attttgtttt gatactcttt
    17641 tccagcagat caaaatcgcc tgttagtaga tcagtgcgtc caatgctgcc cagaaaaagt
    17701 acatctccaa caattacaaa tctcccaaaa tgatttacaa aacacacatg accatcaaca
    17761 tgacccggag tataaagcac ttccaaagca aagctcccaa cttttattgt atcgccatca
    17821 tttataaaat tcattggctg aacgatttgg tcaatatcaa caccaaacat ccgaccctgt
    17881 gatggaacgg tttgcaatat cttgagcgat tcaggatgca tcagcggtaa tagaccatat
    17941 tgtttattta catgagaaat acctaggaca tgatcaatat ggcaatgtgt gtaaagatgt
    18001 gcgactggtt ttaaactatt atctgaaata aatttgtcca gctcatcatt ttcctggaca
    18061 ttgctacatc cagggtcaat tattgcacac tcgccgcttt catcataaat aaggtatgta
    18121 ttttcttgaa aaggattaaa tactaaacgt tcaattttta tcatttttag gattactttg
    18181 tttaatcatt aaaactaatg ccgttattcc ggcaaaatat tttgaaattt acaaagttat
    18241 caatattcaa aattcagtgt gaaacgttta ccagattaaa aagttcataa ttctgcatag
    18301 gttaattatg ctgccaattt atacgcgttt attagctttg ccgaagcctc gattgctcat
    18361 cgtcgggctt ctgttattcc ttttgccaca atttggcaaa gggcagtttt atgaaggcta
    18421 tcagatggaa tttgggcgta gccgggtaca atacaaagac ttcttttgga cttattacaa
    18481 gttcgacaga tttgatatct atttttattt aaatggaaaa gaacttgccg agcataccgc
    18541 gcaatatgta accattgagc tgccacagat ggaaaacaaa cttggaacct acataagcgg
    18601 gaaggttcaa tttattattt tcaacaacct taatgagctt aaacaaacta atcttggcgt
    18661 aaatgccaat atagagtaca ataccggagg agtatcacat attcttggca acaaagtagt
    18721 tctttatttt gatggctcta tcattaattt cgaaaaacaa atcagacaag gtatcgctca
    18781 tattctgctg cagaattcca tctttggcag caatatcagc tcccaaatga tcaattcgta
    18841 tttacaaaca atgcctgaat ggtttaccct tggattgatt tcatatcttg ccgaagagtg
    18901 gaataccgat attgataatc ggataagaaa cgccattgta tcagggaaat acaagaattt
    18961 caatcgtttg gtaagtgatg aattgtatgt aaaagatgca ggtcattcgt tctggaaatt
    19021 tattgccgac aaatacggca atgataatgt gataaatatt gttaacatga ttagggtgag
    19081 ccgtaacctc aatacaggat ttcaatatgt tttgggcaaa acagttaaat cattgtatgc
    19141 tgagtggttc acttatttta ataatattta cacggccgaa gccaaaaagt ttgatcccat
    19201 acctgacgat gtacgcatca aaggcaggcg cattttaaaa agaaacaatc atcaacgttt
    19261 tttttcacaa ctcaaaagct catccaatgg cgaacacata gcctttgtta ccaacgaaac
    19321 cggcaaatat catatctggc tttatgatta caagacacgt aagctaaaaa agcttcgaac
    19381 aggaggctat ctccttgatg aaaaggttga ctactcctat cctatccttt catggcaccc
    19441 gacaggaaga attcttagtg cgatcgttga agataaagga cttaatcaac tctattttta
    19501 tgaccttgtt gaaaaaaaat ggtcgcaacg taaccttttc ggttttgaaa aaatactgga
    19561 cttttcatat tccgacaacg ggcaaatttt gttgatgtca gccgtacaac aaggacaaag
    19621 cgatattttt acctttaatt taatttcagg gagttacgag cggttaacca atgattttta
    19681 tgacgatctg aacccaaggt ttatagaaaa ctcaaccaaa attgtcttta gctcaaaccg
    19741 caaaagcgat accctcatca tgggagaaaa atccgatttt gaaaccgtaa gcaagaatta
    19801 caacatattt ctttacaact actcaaccag aagccaggcg ctgagaataa ttacaaatat
    19861 taaagatgct aacgcaaaac aaccttttac ttacggaaaa aattatctga cctacttaag
    19921 tgataaaaat ggcatttaca atcaatatct cggaagaatt gacagtaccg tagcttacgt
    19981 ggataccgca gtacattatc gctatttcac ccattcattt ccaataacca attattcaag
    20041 tagcatatta tctcatgaca tttctccaaa aggaatatcc aatgcattta taataaataa
    20101 taaaagattt gagcagctat tcaagaaaaa tctattatta ccggaacact taaaaccggt
    20161 tcaaactacc gcaacctatt atgctgatca aacaatttcc gacacaaaat cctttgggct
    20221 gctgtcgggc gaaataggtg aagggattga taacattgaa gacacacatg atcggccatt
    20281 attacatcgc gcaagaaaaa gttttaaaaa cgtaatgaga gatgcggcat caagcgctga
    20341 ttttgatcct gaatatctga tagacacttt attgaatgat caagtggcaa cctttgatcc
    20401 ggagtttaaa atgcaaggca caatcggcat aaacatgccc gatagcttaa atgcaatgat
    20461 ggatcgtttt cgcaaagagt taaaacaatc agactcaacc aagaaattta cttttcccat
    20521 tcagcgtaat tattacacag aatattcaat aaatcaaatt gttactcagc ttgatttttc
    20581 ttatctcaat cagatgtatc agcctttttc tttcacaaat tctccttatt actcaaatcc
    20641 cgggtttagt cctactttta aaattgggat aaccgacctg atggaagatt accggattat
    20701 aggaggcatg agattagaat ttgatctggt taacaaggaa tttttcttgc attttgccaa
    20761 tttaaaaaat cgttttgaca aagagatcat ttttcagtat aggaatcttg aagaggttat
    20821 cggctcaaac tatattattc gacaaaaaat ttatgagggc tattacattg ttacctggcc
    20881 attcaatcgt gttttaaggt tgcgaactac actacttgcc cgtaatgaga attatattat
    20941 tacaggtcct tatgaacggt tactcaaagc gccaaatgtc atgtttaact ggggtggcgc
    21001 caaaattcag cttatttacg acgattcgcg agagcttgga ttgaacctac cgcaaggcaa
    21061 caagtttatg atctttgggg agtacaatca gaagatagat cgctcgggta ataacctggc
    21121 tgttttagga tttgatataa gaacctacaa acgccttcat cgttctttta tctgggctaa
    21181 tcgtatagcc ggaagcacaa actttggcag cgaccgcttg atttatttca tgggaggaac
    21241 tgatggatgg atagcggcaa aatttgaccc tacaacccga attgacccag atcaaaaatg
    21301 gacttaccaa acgcttgcca ccaatatgcg cggttttaag caaaatgctc gtaatggcaa
    21361 taatttcata gttttaaatt ccgagttacg gttcccgctc ttcacctatc ttcttaatag
    21421 acctatcaac tccgatattt tgaaaatttt tcagttagta ggttttggag atttgggaac
    21481 agcatggtca gggtggaatc cttatgatga aaacaacgtg ctttacacca aatacgaata
    21541 ttttggacct ctccgaatta aagtacagta cgaaaaagac cctatcgttg gaggtgtagg
    21601 atttggagcg cgaacaaagc tactcggcta ttttattaaa ggtgatctgg catggggaat
    21661 cgaagatgga aaagtaaaaa aaacaccaat gttttacttt tcattaagtt tggattttta
    21721 agtccactcc aaaaaatatc tttaaccaca aatacttgaa ctttgatcat taaccatgaa
    21781 caaatattaa atatgtaaac cattgaaact atgcaatatg ttcaaatatt aattatgcga
    21841 gtatcaaaaa aactttgttt tcagggcaga cttattttta atcttttaca gaatctttga
    21901 aaaatagact gttgttactt tccctctgct ttttaaaatc aaataatatc tgttttcttt
    21961 tctgaattac ctttgtgccg tcaaggaagt attaatttta tttcaaaagt catactatga
    22021 gcgattcttt acatcatgaa tgcggaattg cgcttttaag attactcaag ccattggaat
    22081 tttatcaggc taaatacggc actccttttt attcattgtt gaagatacat ctaatgatgc
    22141 agaaacaaca caaccgaggg caggatggcg ccgggcttgc aaatctcaaa cttgacatcg
    22201 agccaggcgt taaatatatc aaccggtacc gttccaattc accgactccc ataattgata
    22261 ttttcaacca tatctatcgg ttgtttagca aaatccaatc atcccacccc gaactactaa
    22321 atgatgttgg ttggctgaaa aaaaacatag atttcaccgg cgaactgttt cttggtcatc
    22381 ttcgttacgg tacatatgga gggcataata ttgaaaacgt acatcccgta ttacgtgaaa
    22441 acaactggat gacaagaagc cttgtattgg ccggcaattt caatatgacc aatgtagatg
    22501 agttattcaa ccaattgatt gagttggggc aatatccgat agaaaccacg gacactatta
    22561 ccattctgga aaaaataggc cattttctgg atgaagaaaa tgagattcat tatcagaaat
    22621 ataaaaagaa gggtttggtt aaaaaagaga taacggataa aatagcctcg gatcttaata
    22681 ttaaaaagat acttagtcaa gctgcaaaat attgggatgg agggtatgtt attgccgggc
    22741 tgcttggcca tggcgatgca tttatcatgc gcgatccctc tggtatcagg ccggcatttt
    22801 attattttga tgaagaagta gccgttgccg cctccgaacg tccggttata caaacatctt
    22861 ttaacgttaa ggcagaacaa atacaagagc taaagcccgg tcatgtgctt atcatcaaaa
    22921 agaatgggcg aatcactgaa gaacaattca ccaaaccaaa acaaaaacag gcttgttctt
    22981 tcgaaagaat ctatttctcc cgcggaaccg ataaagatat ttataaagaa cgaaaaactt
    23041 taggaaagct acttatgcca tccatcctta aaagcataaa ttatgatttg gagaacacgg
    23101 tattttctta tatacccaac actgctgctg ttgcttttaa aggattgaca caagagctta
    23161 atatttttta taacaaactc aaaactgacc aaatcttaga actgggaaaa gatgcaaatg
    23221 ccgaaagaat aaataaaata ttaagtagtt ctttacgaat tgaagatatt gccgtcaaag
    23281 acatcaaact tcgcactttt attactcaag aaaatagccg taacgatctg gcaggccatg
    23341 tgtacgatgt tacctatgga ataatcaaag agggagttga taatcttgtg gttattgatg
    23401 attcaatagt cagaggcact acactccgac agagcataat tcggatactc gacagattag
    23461 ggcctaaaaa aattataata gcctcttccg ctcctcaaat ccgatacccc gactgctatg
    23521 gtattgatat gacaaaactt agtgagtttg tagctttcaa agctgcaata gaactattgc
    23581 atgacacacg gaaagaatat attataaacg aagtgtacaa aaacgcaaaa gcccaggagc
    23641 aaaagcccaa agaaaagatc gtaaactatg taaaaaatat ttacaagcct tttactgcac
    23701 agcagatttc tgaaaaaatt tctgaaattg ttacaccaca tggtataaat tccaaaatag
    23761 agatcattta tcagtcaata gaagaccttc acacagccat tccaaataat cgtggcgact
    23821 ggtacttcac aggcaattat cctactcctg gcggtaacaa ggtggttaac cgatcattca
    23881 tcaattacat cgaaaatcga aacgaaagag catattaacc cgaataatgt tctatatagt
    23941 cagctaactg attaagctcc aatccttctg atgttttttt aattggctta atctttcttt
    24001 aagaatatga ttgttgtttt ttactaaatc aggatcgctt ttaaggattt cggcagctaa
    24061 atcgcgggca tatcgtaaca ttttttcgtc gcgaatgatg tcggctaatc gaagatcgag
    24121 catcccactt tgccgggttc catgcatatc gcccgggcct cgtagtttca tatccacctc
    24181 ggcaatttta aaaccatcag tagtttttac cattgtatca attctttttt tgccatccgc
    24241 cgtaagttta tagcttgtca ttaatatgca aaacgactgt tgtgctccac gtccaaccct
    24301 cccgcgcaac tgatgaagct gtgataggcc gaaacgctct gcattttcta tcaccattac
    24361 cgaagcattg ggaatatcca ctccaacctc gatcactgta gtagctacca taatttgagt
    24421 ttcacctttg ataaaacgct gcatctcgaa atccttttca tcagttttca tctggccatg
    24481 cacaatgcta atcgcataat caggcaaagg aaaagttcga gaaaggcttt cataacctgc
    24541 cattagatcg gctaaatcca aattttccga ctcttttatt agtggataga caacataaat
    24601 ctgctgccct tcggcaattt tcctttttaa aaaaccatat agcatcagtc gtttattgtc
    24661 ataatagtga taggtttgaa caggttttcg accgggtggt aattcgttaa ttacagaata
    24721 atctaaatct ccatataccg tcatggccag tgtacgtgga ataggagtag cagtcatcac
    24781 taagacgtgg ggcggcatct catttttttg ccaaagccgt gcacgttgtt caactccaaa
    24841 gcgatgttgt tcgtcaataa ttactaatcc aagattttta aattgtacag tatcttcaat
    24901 aagagcatgt gtaccaataa ttatgttcac gacgcccgac tctaatccat cgtgtatttc
    24961 ctgcctttca gcctttcggg ttgatcctgt tagaagtttt gcttttaaat ccattccgag
    25021 cagtaaatcg ctgatagttt taaaatgctg ttgggcaaga atttcggtag gcgccataag
    25081 gctggcctga aaaccattat caatagcaat gaacatacac ataagcgcaa ccaatgtttt
    25141 accgcttcca acatcacctt gcagcaatcg gttcatctga tggccacttc ccatatcagc
    25201 ccgaatctct ttaataactc gtttttgagc gttagtcaac tcaaaaggga gatagcgctt
    25261 ataaaatgta ttgaaattgt cgccaacgag tttaaaaaca tgtcctttaa gccttattgt
    25321 tctaacatac ttatacctga gtaatgcaag ctgaataaaa aatagctctt caaactttaa
    25381 tcttgtagtt gctttattga gcaattcctt attctcagga taatgaatat taataacggc
    25441 agtatggcga gaaacaaatt taaatctatt gattacctgt ggcgaaagag tttccggtaa
    25501 agtttctatt tgcgaaatca gcaatttcgt gagcttagca ataccacggc tattgagatt
    25561 ttttgatttt aatttttcgg tcgaaggata aaaaggccta agttgcgaac ttaaaatatc
    25621 aaaatcctct accggttcaa tctcaggatg tgaaaggtta aatttgccat taaaataatt
    25681 tggtttacca aaaacaatgt attcaacgtc ggccttaatt ttttctaacg cccaattaat
    25741 cccttgaaac cagaccagct caataatgcc tgtatcgtcc tttaaatatg ctgttaatct
    25801 tttcgcacgt gcagcgccca ctatctgtgg ttgaagtatt ttgcctttta attggaaata
    25861 agtctgatca ttatcaattt cattgatatt cgaaaaagta ctacgatcaa cataccgaaa
    25921 gggaaagtaa tttagaagat cgtcaaatgt agaaatattc aattccgatc tgagtagtgc
    25981 ggcacgcttt gggccaaccc ctttaaggta ttcgattggc gttttcaaaa attcgtatga
    26041 catactcgcc gatttgttga atttacaaaa ataaaaaaac tccctgagta tttagccttc
    26101 agggagtatg tcattttgga ataatttcag aaaaatcaat aggcccaaag ttcttgttca
    26161 aagtctctga tttcgttttt aatccgttcc gattcaagta atgcatcaat accggaggtg
    26221 tacgaatcta ttctacgatc gaatacgtta tcctctttaa tgatatagct gctaaaccta
    26281 cgctgcataa acatttgatc gtatgacaag cgacgtgcgc tattaccaga gttaaacact
    26341 tcattttgcg ccaataaagg tcttatttcg gggaaataaa gccaaaacaa aggtttatac
    26401 cctctaaaaa ggttcgttgt aggatctata tcttctttaa caggacaaac accaataata
    26461 cgcacctgca tttctgaacg ttgtttatca aaaatccagt cttctttaac ccatagaaat
    26521 ttcacatcct gtgggtcaaa ttcaatatca actgtttgcg taataaatgt tccgggatta
    26581 tttggatctt cctgcctgat tgtttgttgt gtagtaagat ttgtttgtaa ttggctgaca
    26641 ggcaccggta acatttcttc aaaattatca gagcttcccg ttgcatcgta agcagtaata
    26701 tcgccattta gcatagcatc ccatactact tgcataaagc ttctccactt gccttgggga
    26761 gtttgcggga aataaaaaaa ctggttcatt ttttctctga aatcaataga agtccatacc
    26821 cgtttttccc aaaatacatc cgactcgcgc aaatatgaat atggtattgg tttcagatca
    26881 ttgatatgaa ctctatcata tgctctatct cgcggttctg taactaactg aacttgactg
    26941 ccaggctggc taaaagaaac ttttaacact aacaggaaaa ttcctaacag taaatttttt
    27001 actatgtgat ttttcatata tcgtgaattg ctttattagt ttaatgttaa tgaaataggg
    27061 ttaagcgggc gatctgttcc gtctggccct cgagcaataa tatcttcgaa ccaaacccta
    27121 tcgcctctgt tagcatttct gattcggttt ttcatctcat cggtaagtcg gttactattc
    27181 gatttaaaat cagtaactat tgaccctgag agggttgtca ttttaaaaga ggtgattctg
    27241 aaattaaggt caaattcaaa atcctgcggc atgcttgcct caatagcatt tacctgcgcc
    27301 aaaacgtttc gatttatagc tccctcttta tatcctgcta ttaaggcggt tgggtcgggt
    27361 actcttttta ttctgtaaga gttatcacct tgttttttca attggccatc aattcgtgcc
    27421 gaaacagtaa caattgcctc ttttttatca acgggaaccg ttacgatata atttccgggt
    27481 ttgttatctt ttcgtatgtg acctaccgaa attgttactt caagattttc gagtggcaca
    27541 ccgggggaag agactgagat tggattgtca acaccggcat aaaatacatt cattttagtt
    27601 gcagaaatgg tagttgtagg ccttgctact acatagttag cagaaaatgg atagctattc
    27661 acttcacctg tgccggtagt aagcctaaaa attccagcaa acctcttaag cccttcggtt
    27721 tgtgctggga aattaacttt aactactcct ttttgacctg ctatgggtct ggctctattt
    27781 atttgctcat cagtcattct atcggcgcct tcaagtatgt aaacttccgg gtcctgacgg
    27841 gtatcatagg cggctacaag gatttcagct tcgtatcggt cgccaataaa tacatagtct
    27901 ctttttggaa gaactcgggc tgctaccgta tcatatttaa aatcagcggc atcaattgaa
    27961 gctaacagat agttgaccac atcaaattcg gcattataaa cctctgtggt cattttgttt
    28021 aaaatagcca gattcgctgc taatacggta tgataaaaat tgtattgaac ccagttcaga
    28081 tgatttccat cacgatccaa ataattccca tcggtctgaa ggccgatttt aatttgtcct
    28141 ctattttttt catcaacaag acttagtatt ttttcacgat agttgatgat tttatttttt
    28201 agtttaatac cctctccttt agttccatcc gttgaacctg ccataaaaaa gttagtagta
    28261 gtagtaaaat catccagttt tgccaatttt tttaccggaa taagctttgc cgagtcataa
    28321 ggaactcttt cagtaacttc aataagccgc catttaacat cgtcaatgta attgatcatt
    28381 tcagtcgaaa gcctttttgc ttctatcgcc ttgtcacgaa atggctttac cttattttgg
    28441 tttatttgat actgcttgtc gaatgtattg tagagttcat ttaatttttc agaaaaattt
    28501 tcatttgtaa gagctacact ttcatttaca acaacaaacg catccaacac ttctttggaa
    28561 acattaagtg caagtaatgc cgtgagtaca aggtacatca tagcgatcat tttttgccgt
    28621 ggggtctctt tataaccagc cattgatttt gggtttagat tattcgaaaa agctatttaa
    28681 tgttcatggc ggaaagcata ttgccataaa cactatttaa agaagccatt cttttggtta
    28741 acatctccat ctcacgctga tattgatcag ttttattaga agattcctca aggttggata
    28801 ggaatttatc aagcgtttgc tgcattcttt tagaagcttc gacttgtcgt tcagaattca
    28861 caagttgcaa ttcgtaaatt ttatttaacg aggctaattt ttcagagagt gtggccaact
    28921 gttcgttaaa tgcatcgcta ttacctgccg attttctaag ctcctcaatt gttcttgaaa
    28981 tgccttcggc cgacatatag tatgaatccg acaatttaga gacagactct gatgtggtct
    29041 taatgacaag agataagtcc tcggaggcac gaatatcctc tcttaatgta cgagcggctt
    29101 cctgataaga atttttcaat cctgatgcgg catgtgatac ttcttgtacc tgctgagtta
    29161 attcgtttgt agctttaagc tcgttatcta atgcgtttga tgtatctcta taggttttgg
    29221 ccaacgtaga aaccgattga gaagccgttt tcatgttggc aacaaagtca tttgtggcaa
    29281 tgcttgcatt agaaagatca ttaagtttcg atgcactctc actcaatttt gtaagccctg
    29341 taccgaggct atgaatcaca ctgtcatcca atttaacatc tttcaacatc aagtccagtt
    29401 gtgtcatttg attacttccc gttctgacag aagctgattt tctttcaaaa ggagtgtcgg
    29461 gttttattcc atggtaatct tctattagtt ccggatacac tttactccaa tcaggctcaa
    29521 cgtgaggctt ttgtaaagca gagagtgcga aaataatagc ctcggtcatc atacccatca
    29581 aaagcatttc attggcgccg gcatagtgat tgattttaaa aagcgcgcca agaataacaa
    29641 cagatgcacc taaaccgtaa gcgactgaca taaaccttct gaaaaatggt gtttccaact
    29701 tttcactaaa tgattcttta gattttactt ttgtagtcat ttccatattt taattaggat
    29761 attttaatta aatgatttga agaatattaa aacagtttta tttttatcta ctaactaata
    29821 aacttttgat gcacgacgtg ggttatcacc tcgtgacctg cctaggtagg acataacaca
    29881 cctaaagcca atatacgatt tagctgtatc ttgatattca taatcctttg tagagactaa
    29941 aagataatac tcaatatctt tccatgatcc gccttttact atttttcgtt tcatggaaat
    30001 aggatcatcc ggctttgcta aataactgta gtcggggttt aaatcccatg taaaaaagtt
    30061 tgccgattca tcaaaagcgc tgttagtcca ttcagctaca ttccccgaca tatcaaatag
    30121 tccgaaacta tttgcctgat aatgccccac aattacagta gtagcaccac catcatcaac
    30181 ataatttcct ttattaggtt taaaatttgc caacaagcat cctttatcat ttctactata
    30241 tggccctccc caagggaacg gattagcctc gaaccctcca cgggcagccc actcccattc
    30301 ggcttcggta ggaaggcgaa aatcattaat gccagcccgt ccttttccct gtaaataact
    30361 gttgaacaat gctgttctcc atacacaaaa agctctggcc tggctccaat ttacaccaac
    30421 aacagggtaa ttatcgtagg tgggatgcca gaaataagat ttggtaagag gttcattata
    30481 gctataagga aagtcatgaa tccagcaaag tgtatcagga tacacattga tagtttcttc
    30541 ttttacatat ttagtcctcc cgtccggacc aatctcacga ttggcaaaac ttgcatcttc
    30601 ggggtctccc ttgttggtaa aatctttacg tgcagcagat tgcaaatcaa tccagaaata
    30661 tttatatttt aattttcgcg tgtcaatttc tttctttctg aaaaaacgct cttgctcagg
    30721 aaggtacatc tcttctaaag tttgagctgt ctcctcgtca tcccatttaa tatcggttcc
    30781 ccaatttaaa aacggaggat cataaacttc acctgttttt ttgttttctt caataagata
    30841 ggcatccgga tttacctgcc caagaaggtc tctggcaata gaatctcgga cccaatgcac
    30901 aaactgacga tattcgttat tggtgatctc ggtttcgtcc atatagaatg aattgacggt
    30961 aactcgtttt gggttattaa tctgagcata tggaatatcc tcttcaccta cccctaaaac
    31021 aaagcttccg agtgggatga aagccatccc gtatgggtct ggctggctga atgatgggcg
    31081 ctttgtaaca cctaccaatt caccgtttcc tgtattttta cacccactaa ttaaaattag
    31141 ggctagaaaa aaaaacaata atctcttcat gtgtgctgct gattttacag tcaggttaac
    31201 gattttatgc attttttatt attgttaatt atttttgatt ccttattttc aacaagttct
    31261 gattagtttt taagacattt ctcgcaaacg attttcaaaa gagataaaag acaaggtttg
    31321 ttttataaga atcttgtatt tctataactt tgtttatatt tatcaatctc aactttaaag
    31381 caataattta ccctaacctc atggctacca aaagaaccaa ttgctgaagt tgggatatca
    31441 taagagtaac ctatgctcaa ctctttgaac tggattccta aaataatggc tgcggcatcc
    31501 tgtaagcgat acgtcagccc tccccatatc ttattattga acctgagtaa ggctgaaaaa
    31561 tcatattgtg ccgaagtacc gtcggttttt aacatcactg aaggcaaaat gtcaatagaa
    31621 ggagtattcg gcaaggtaaa ttcatagcca cctatgacat ttatctggcg tttaagccgc
    31681 attcttgttc catcattttt ctttgcttct cgtaattgca gcaccgaaag gccgagataa
    31741 tagttatcgg gaacgctata gtaaaatcca actccaccgt cgaaatacat atcagactct
    31801 tctgcttcac tttgcaaaac aggatcagaa cctttaccgt cgggagcttc gaggttacta
    31861 aaattcaatg atttattaat tatacttaat tgcaatccaa tgcccaaatc accagcgcct
    31921 aacattgttt gatatccata agtcagatta accgatatat cgtcctggtg cccaatctta
    31981 tcctgaataa tggtgccacc caaacctcct cgtagcgctt taacgggtgc atttatggta
    32041 aacacataag tttcgggagc aacattaaaa gaatttcccg ctgcgttggt ttctttaaat
    32101 cccacccatt gctgacggat aagacctcca acacaaatac cgttagtagt accggtaacc
    32161 cctggattat atgcaatttt attaaacatg tattgactat actgtggtag ctgttgcgag
    32221 aaaccaacaa tcccgaaaca gtaaaagaaa atcaaaacag tataaaactt acttgtcatc
    32281 aatacaaacc aaaattaatc aatagcctgc aaagaaaata aaattcattt taaccattac
    32341 ccaaaccaac tgctacgagg tttaatctga ataaagtgtt tcaaaaacga atttctttat
    32401 ttgatattgc tgaaaagtac tatttgaaac cctaaaatta gatttagtat taaagcaaat
    32461 cctccaaatt atttttaatt atgacatttt ctgaaagatt taaatcgcat attgtctcta
    32521 acgctttatt aataaaatcg tttgtggtat ctatatactt tatcgaaagg ttgatgccgg
    32581 tattttcaaa aaagataaat ccgttcttag ccataatttt gaaagtttca gtcgttgaca
    32641 ccatctgatt agtatatttt ttaataaggg aagcggctac ctgctcaaca ttaactttgg
    32701 tagcatcgga gaaaaacatc tctccaaaaa aaaacttttt atcaatgaaa tgatagataa
    32761 atcgtattcc ggtattaaaa accctttctc tgtatcctaa cctttgccaa taaaaatcca
    32821 gatacttagt aatgtcgtaa caactgtacc ttttgttttt ccttaacaat gacctaaaag
    32881 ttattccaaa tttattattt tgaaaatcaa tccgtttgtc agtattaaat actgaaattt
    32941 tatcggatgg ctgaatggcc cctttgtaat gatttaacgc atctaaccca gcacaaggta
    33001 aaaaatactt ttgccgggtt aagttccgag ttttcaggcc aatgccatat tgttgaaaaa
    33061 cataactaaa gcgaccatat atcaacctta atatcaacaa aataactctt ttcatactct
    33121 aactcttcag gagattcagt aattattaac tatctattca gattatttat tacccaactt
    33181 aacacatgca aattattaaa tacatctttt atcattttga aaaataaaaa attcaataat
    33241 atgatatgtt ttgtaccggt tggataaaag attaatttgt ttttttatca taattgttta
    33301 gcgggtttat cgcgaaaaat gttgtgatgt atcaaaatat caatcgaatc ttttttttta
    33361 attttgtatt agcttacgaa aggtaatttt agccacaaat actttaactt tgttcagtaa
    33421 ccatgcacga atatctaaaa aatttaattg tcggggccaa gtcaattttg tgttacaatg
    33481 ttgatgcgta ttatcttatt agcatgtcct taaaaatatc ataatagcat ccttagtgat
    33541 tagcattcaa ttgattaaag gcaattaagt tattgaaatt cgcaaaaaac attaaaacag
    33601 atgaaaacaa tcatcatact taaattctcc tttagagagt ttaaaaaaag ctttttactt
    33661 tttttgttca ttctaactac tgttgtagca cactgtcaac aaagtggcgc tgggcaacaa
    33721 tatcagaatc aatcttatgt aacttttcct gaaaatgtta gagctcccta tgaacttcca
    33781 gcaatctgga gccatcaaaa taaaacagag aatccacatt ttataattgt ttttttagaa
    33841 gcaaacccaa ggattaacga tatcccttta gagccaggtg attttatcgg agcattttat
    33901 accgacgatt atggaaatcg aaaatgtgga ggcgccgatt actggcttgg cgatgagaat
    33961 attattttcc cttgttatag agatgatgat tcgacaacag aaaaagatgg ttttttacct
    34021 ggtgaacaaa tatattttaa gttgttctgt tgggcaactc aaaaagaata tgatgtaacc
    34081 ttagtcgttt ttgatccttc atttgttact actgataaat ggtatcctct tggattaagt
    34141 ggcataataa acattgcaac tttcacagtt tttgacgcct acgccacggc tacgcctaac
    34201 cctatatgta ttggcgatga agtaacctta agtgcaaata ttttaatagg aactaccggt
    34261 aattacacct atttgtggac atcagatcct gccggactga attccacact acaaacggtt
    34321 acccaaaacc caacagttac tactacctat tttctctctg taagcgacgg aacaaatatc
    34381 tcacaacata acattgaagt tattgtaaat acgcctccat ggataacccc cggacctgat
    34441 caaacaattt gtgaggatca aacagtgtca gtaacagctt ttcccgaaaa caattcaggg
    34501 attctttggt caagcagtgg agatgggagc ttcaacaacc catcattgat aaatccggtt
    34561 tatacaccag ggacagccga taaggcaaat ggaaatataa cccttattgc tacagctcta
    34621 ccaaaagcgc cctgcattga ggttgctaca actgatatat cggtaaagat caaccccatt
    34681 gcatctcttg atctaattga aaatatctca ttctgcaaaa ttgatgagat gtggataagc
    34741 gctgaggcta ccaactactc ctccatagaa tggacaacta atggtgatgg cacaataata
    34801 aatccaaatt caactactat ccgttacatt ccaggaagtt ttgatctctc tttaggatat
    34861 tttaagctca cttgttgcgt gaattctatt gccccttgta cccaaaatgt ttgcaaacaa
    34921 acacttgtaa ccattgtaac tcatcctaca gttaattcac cagtaaacaa gaaaggttgt
    34981 gaaaacacgg caatatcatt aaatgctatt gctaaaaact atagttcgat attatggata
    35041 actaatgggg atggggtatt taataatcca aacattttaa acccatcata cacgcctggt
    35101 gttaatgata aactcaatgg tggtaccgtt gtaaccgtaa ctgcctttgg aaacattggc
    35161 tgcgaggctt ttcctgcttc aagcaacacg acgataatta ttgagtcaat gccaaagttg
    35221 aatccaggaa ataccggtat tttctgtaaa ggatatccct tgcagatgga tgcttcggcg
    35281 caattctata actcattaac ttggtcaaca tctggagatg gaactttcaa taataaaaat
    35341 attcttaatc caatttatta tcccagcgct aacgaccttg aaacgaattc attcgacctc
    35401 acgctcactg caacagctaa gtccccctgt acaggaagtg tctctttagt gttacccatt
    35461 actctcgtta cagaaactac tgccctaata ttaacagcag ccggactgga attgtgtaaa
    35521 ggagataccc tacagctaaa ctcagaggca acaggctata cttcaattca atggactact
    35581 acaggcgatg gcagttttga cgattctaca tcgttgaatc caatatattt tccaggccct
    35641 gtaattgact actcaggcat tcccatccaa ctgaaactaa ctgctttcac tcctcaaaat
    35701 tgcggtccca atattgaaaa aaacattcag gtttcattca atctatacga gccagaggtc
    35761 tttattggtg aagatgctac cattaccgaa ccctcaacat ttttcacaaa ccctgttgta
    35821 ccaaactaca cttctttgtt ttggcaaact tcgggcgacg gacaatttga aaaccccaat
    35881 aatctcataa cagaatatat tcccggaatt cttgatcttc aaaatggatc aacactcctt
    35941 attcttacta cagtaggaca aggtaattgt tacgggccag cattagacac actcatgcta
    36001 accattttta gaaaacatca aatagagatg gaaacaggct ggcaaggaat ttcatctttc
    36061 gttactccgt tatcaagtag ttttgaaata cttatgacac agatttccaa tcaattggca
    36121 atagcccaaa atatgttgga aacttattgg ccagaatatg gtattaatac cattaatgat
    36181 ttttccaatc aaaatggtta tcaagtcaaa ctaacctcgc caacaacact tgaagtcatt
    36241 ggatacgatt tcaaacctaa aacagtgaat tttgattttg gctggaatat catgccggta
    36301 gcttcaggat gcaatattct atcttcaact atcaccgatc aactgggtga taaactgata
    36361 attattaaag aaataggtga acagggagtt atttggccac agcagggtgt ttatcaaatt
    36421 ccttatttta ttccaggaaa agcttatatg atcaaagtta cagaaccagc aaccgtaact
    36481 tttccaacct gtactaaccc gaaaaaataa agagctaata gcgtttgctt atcttttaac
    36541 ccatggttaa tatctgacaa tgaccaaaat aaccagtctt cagctaaatc ccaccaaatt
    36601 attgaacatt caacatttgc agtgaaattg atatcatttt ccgcaatatc tttttataac
    36661 gttgtatgct tcaacaatac ctaactcgcc ggcgctgctc aaatcaaaac aagcctctgc
    36721 gttgcggtta agatagatca aggtaagcgc ccgattgtaa aaagcctcag caaattccgg
    36781 attcaaagca acagcaatgg tataatcttt aattgcacct tcaaaatcaa gcattctatt
    36841 tttaatattt gctctgttaa agtatgcgaa atacatgtta ggattaagtt ttatagcttt
    36901 gtcgtaatca gaaatgactg atgcaaaatc gggtatttta ttttctctct cattttgcga
    36961 aagggcagac ccatcggcgc taagaaagat tgtcggttcg gtatagtcaa aggaatattg
    37021 aagctcaatc aattcaaaaa gaacggtagc tctactgaaa taggcttcaa aataatcagg
    37081 atttaactca atggctcggc taaatgaagt gtaggcttct gaatagttca tcaacatcca
    37141 gttcagaacg cctttaacaa aataattatc ggcatcaaac gggttaaaat acatgatact
    37201 atctacctga tcagaaatca tcttggcttc ggcaacggag agtgaaactt tttcgctatt
    37261 gattccaagc gaaaataagt cggaattgta tttaaactct ttcagtggtt catatacata
    37321 gcctctcttt ttctcatcca ggattacctt ttccgaaggg taataataga tcatcacatt
    37381 aggtttaagc tcaattaata catgttgata ttgaattctt ccttccaaaa tattgtaact
    37441 attaaaatca gcttcaaatt caattattct tttaaggtag tcggaatcgg ctgaatattt
    37501 ctgatccaga aggctaaaat catcatcatt caataaatta ataatggcta tagcatattc
    37561 ataatcttcg tttgcgcctt gataatcttt tagttcctgc tttgcggaag ccctgcccat
    37621 ataagctgct gcaaaatcag gaaagagttt tattgcttcg gaaaaatcat caatagaggc
    37681 attatactct ttgagattta agttgatgat tccgcgatta taccaggtaa aaatattttt
    37741 aggattaatt tttagtacca tatccatgtc tgtcagcgca tctctaaact ctttccggct
    37801 cgctttaatc aacccgcgat tataataagt caggtcgtta taaggatcta atgctaacac
    37861 acgatcaaaa tccagtattg cagccatcgt atcctgtaaa ttgatatgca aagccgcgcg
    37921 atagaaatat gcaagcgtat tattggcttc aagcttaatt gcctgatcaa aatctctgat
    37981 tgcttcctgg tattcaccca tcattttgta aaacaacccc cgttttacaa aagcatctac
    38041 ataatagata tttaatctga tagccttatt acaatcttgt atagcgcctt gtagatcatc
    38101 attttcagct tttgcaattg ccctgctaag atatgcctgt tcaatatcag gacgatagat
    38161 gattaaggta tcaaaatcac tgattgctcc ctcaaaattt ttcatcctga ttttggttat
    38221 cccacggctg taaagaacat taggattatt aggcctacgt tctaaagatt tgctaaaatc
    38281 ctctaacgca ctgaaataat cgaaaaggtt ggaacttact acacctctgt aataataagc
    38341 ttcagggtta taattatcat tcataatagc acgggtaaaa tcaaattcag cgcccttcaa
    38401 atcacctaaa gcaagcttcg ctcttcctcg caataaatag ggctccgaca aatcaggcct
    38461 accacgaact aagatattga acgtttgtat agcctcagtg aatttttttg aattcagctc
    38521 atcaacacct ttttttctaa aaaagtcaac gtttagttga gcttttccaa agagtgaaac
    38581 gaatatcaaa acagaaagaa ataaatatct aatcagcttc aatgtacaag ttgttatatt
    38641 ttggtttttc agataagcat gatatccgat cgcatgttct tttccctgca ttaataccgg
    38701 catcttcagc tatggttagc gccttgcctt ttactaatga tactcctctt tgcaggattg
    38761 aaaagaacag acaacaaaat gtaaaaaagg atcacaatag gaaatgcata aaacttcaaa
    38821 aataaaacca aaaccaaagc tacaaacaag aaaatatagc gtgttgaatt tgctgtaaag
    38881 cttagtgttt tgaatttaag cgacattaac acaactgaag agaccattaa atatgaaaat
    38941 acaatgacaa gcagagataa aacccagtag ttttctatta aacttttcac catagtatga
    39001 tttgatatgg atgcctgatg taaaactaat ggtaatgatg caaaaaagat agcgctggtc
    39061 ggtgttggca ttccgataaa attttcattc tgccgattgt caatattgaa tttggcaagc
    39121 ctaaacgctg aaaatacagg aattaataag gcaacaaaaa gggcaaaatt tatgttgtaa
    39181 accaaaatca gaggagcgtt gcttgaatta ttcataagat ggaatatgat aaatccagga
    39241 gctaatccaa aggagatgac gtcagccaat gaatcgagtt gcaggcctat ttctgaacga
    39301 acctttaata ctctggccaa cgttccgtca aggaaatcaa aaattgctgc catcccgatt
    39361 aaatatgatg ctaaacgtaa ttcgccgcta aaagcaaaaa caatagaaac gcatccgctt
    39421 aatagattta acagcgttac aaaattgggt atgttttttc gtatcatcga atttgatttt
    39481 atttgcccaa aactttttat gataagtaac agttatttaa aacaagcgtg taaaattagc
    39541 cttttcaatt aaacataata ccaaataacc gatgagccct gaagaattta tgagactgcc
    39601 tgccgtagaa cgggtaacat taattcttaa tgacggcaga gagctaatgg acagaattta
    39661 tctatattac ctcataagac tttacaagat ttttgacttt tacatcgaat tatggtattt
    39721 gcctgcgtcc aataagattg acaaaatttc aatcgttgag ttagatgacg ttatgcatct
    39781 atacgaaaaa aacattgata tctccgatat ttttaacact taatgatggc gttggataga
    39841 ttagttcaat tgaagataga ggatttttgg tacgaactgc ccgaaaataa aatcgcaaaa
    39901 tttcctctgc aaacacgaga tgagtcgaaa ctacttgttt ttaacgggat tgatattcag
    39961 gttgatcatt ttaaaaatct cgccacctat ctgcctaata attccacttt agtattcaat
    40021 aatacaaagg tggtaaacgc ccgtcttttt tttaggaaag aaaccggcgc tttaattgaa
    40081 atattttgcc tagaaccttc aacgcttttc cctgacattc agatcgcttt tcaacaaaaa
    40141 ggcacggcgc tttggaaatg ccttttaggc aacgcacgac gatggaaatc aggtgtgttg
    40201 agcggtaaat tcaatcacgg taatacaaca gtgattataa aagccgaaaa aatctctcaa
    40261 cttgctgacg gatttgaaat acgatttaca tgggaaccag cagatctaac cttttcagaa
    40321 cttttacagt atgctggcga agtacctctt ccaccttatc ttaacagaga acctgttgaa
    40381 agcgataaat atcaatacca aacagtatat gccgaaaaat atggatcagt agccgctcca
    40441 acggctggcc tgcattttac cgatagagtt ctcgaaagcc tgtcggcaaa aagtattaaa
    40501 ccagccaacc tgactttaca tgttggagct ggaacattta aacccgttgg aacagaaggt
    40561 ataaaagcac atataatgca ctccgagcaa ataactgtaa cccgaaagct gattgaagat
    40621 cttttagaaa atataggaaa tattattgct gtcgggacaa cctcagtaag aacgttagag
    40681 agtctttact ggtttggtgc aaagcttgaa aacgataaca atgcaagttt taacattcat
    40741 caatacgatc cttttcatct tgaaacaaaa aatgatacct cagtagaagc cgctctcatt
    40801 aatgttttag attatttgaa aaaaaatcag cttgagtccg taaatggttc tacccgtttg
    40861 atgattgcac caggttacac ttttaaattg atatgcggaa tgataacaaa ttttcatcag
    40921 ccgcgctcaa ccctgttgct attaattgcc gcctggcttg gagattattg gaaagaaatt
    40981 tatgaatatg ctctcaacaa tgattttcgg tttctcagtt atggcgactc ctgccttttt
    41041 attcggcaaa agatttaacc ttttttggtt ttttgatttt ccagcctgtg cccttgtaaa
    41101 aaccaaatgt gcttaaatgt gagatagcct tcgccttgtt gctaatggtt tgcaagtatt
    41161 tgcagtagca aattagaagc atttacctat ccgtttataa caaagttact tggaagcaca
    41221 gactttcgat ttacccttca cttgttattg ccatatacac tgtgttatgg gctgtttttc
    41281 ttctttttaa gtccaaaaaa gattaacact aaacctgcga ttaacaaaaa tattgaaccc
    41341 caaacgtagc cttttccata ttgtgtcaaa atattgtaat caaataaata tctgaaacct
    41401 tgaaaaaaac caatgagtaa caaaactatt cctattatca gagcaacttt cctcatttct
    41461 tttaataaaa aaaagtcagg gctatgacca cataaccctg actatagtaa aaactatttt
    41521 tttactgttg tcgtagttgg agtataaa
//
LOCUS       DAOOAI010000005        40468 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_1259_length_40468_cov_5.070918, whole genome shotgun sequence.
ACCESSION   DAOOAI010000005 DAOOAI010000000
VERSION     DAOOAI010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 40468)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40468)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40468
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1259_length_40468_cov_5.070918"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1452
                     /locus_tag="PK345_00780"
     CDS             <1..1452
                     /locus_tag="PK345_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33587.1"
                     /translation="GAGVVGGTDFNPAFADVGEHLITYEAITNSGIESCTFTITVLDS
                     PDVTCPSDISICITTPEVVLTGGHPSGGVYSGTGVVSDTFVTADAGAGEHLISYTYVD
                     VNGCENACDFVITVVDEPSVDAGPDIIICGDGPVEICGVVSNTSNFVWQVVGGNAMGT
                     FDDDCSICTTFTPSEAALEQGFVIISLVVFPQSPCTLPRVDIMVITFSSAPTAFAGSE
                     GSICYPDLTEWALTDASADHYSSLLWSSTGTGSFDDPSSLTPIYTVSAEDVLVGSVTL
                     TLTAYPIEPCTDPAISSVALYIAPSPTVYAGIDTIICQFECCSGGDAGSYCLSAEAAN
                     YSSLLWSSSGDGTFADPSQLNTCYVIGSDDMVAGVVTLTLTAYPIEPCAEPVQSQILL
                     TIQPAPIVDAGPDATICVGQTYTLSGSSALYSSSLYWDFAYMGEGDGVWDDHTVLHPT
                     YTPGPGDLDRGYVTLILVGLVTILVTALLMLTL"
     gene            1449..5417
                     /locus_tag="PK345_00785"
     CDS             1449..5417
                     /locus_tag="PK345_00785"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPX33588.1"
                     /translation="MKLYFESVPDVSFEAYPVCFGTEVSFINLSSSGTWLWDFGDGST
                     SDLKDPTHLYDAPGVYSVCLTVSVNTGCSDTFCGEVEVYSLPEVNAGSYPTLTIYDDI
                     TLQPIVSGATPLQFEWSPSTYLSDPQVERPIFGPAPLGVYTLTLTVTDDNGCSAQAET
                     IIVVGPSEIIANDDIGTPVNGYDGGVVVANVLSNDLLNGSAPTLSLVDLTIVTPPSHS
                     GIVLDLLTGEVTVAPGTPAGTYTFTYQICEILNPTNCDDALVTVVVTPPDILAVDDDF
                     SGNPINGMDGGVAGNVLDNDLLNDDPVDAGDITISVVSDGGLTGVVIDSDGNLNVPPS
                     TPAGTYTVTYEICEIINPTNCDQAVITIVVTASTIIANDDVGAPVNSDDGGVAVANVL
                     SNDLLNGSEPTLSLVSLTIVTPPSHSGIVLDLLTGEVTVAAGTPVGIYTFTYQICEIL
                     NPTNCDDAMVTVVVIEDPAVLFCFNDVTATTGSEFAYCEGQTITVTLCDVLAGLAPFD
                     ICWEVNGSPYCDYGVGIDDILFSDQLPAGNYEIVITSITDSLGVNVIDVSVYHATVIV
                     HADPSVEAGEDDVVCAGSSYELLGSLASNYSSLLWSGGDGVFSDPTALHPTYTLGAND
                     LAVGSVTLCLSAEPLDPCTVPASDCLVLTIQDTPTVSLDSDGVVCEDEFFALEPVVSN
                     YSEVQWSGGVDGFFLDENDPFTSYYPGEEDIAAGSIELCIEVYAVSPCIGSVTACITL
                     NIEVAPVANAGVNDTICEGDSYHLSTATATNYGSVEWLSSGDGTFDDNTVVNATYTPG
                     EGDIANGSVILLLHAYPESVCTTVAESAMLLTIVGYPTLDMDSEVKLTCEDYDVAAGA
                     WIPFQIDVVASNYGSVLWTTTGDGYFDDPTDINSVYYVGLNDIWQPYFELCLTIEGVG
                     VCNASVSKCVTVEIPQQLIYYDQDGWWGLSSYLDPYDPPVPTVVDPIVLIPGSQHLIT
                     MVDRAGKYFWAEDIPPKNQLGDWKPIGYKIKMKNTPACLPIYGDSLTNQTFNISGLFT
                     FLPVLTNVPVAITDLFADHLNKIRMIYDWPTARLWTEYVSDFDILQPGKAYLLVNKVT
                     SNYSVTFPDYDPSAPHRYPTLKSSNYFTSPWSDIVNTAVPHVVVFDKSVQSKLCIGDV
                     IGAFNHTGICYGSVEFNGVGEFMKLVVMGDAPFSSGTVGFKESEIIYFKLYRPSTGET
                     FNLSFIYDQDFPVTDDHFTENGIARVVDFKSEAIVVSDLDLSNHVIVYPNPASTNLTV
                     ESSKPITNVVMLNSLGQRVLSSDIMGVTGVVDLNGLSRGMYVMHLYFESGHHTIKRII
                     IE"
     gene            5602..7923
                     /locus_tag="PK345_00790"
     CDS             5602..7923
                     /locus_tag="PK345_00790"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33589.1"
                     /translation="MKKLIFIFSLFTFAGYLMAGSPVITVRFANPELNCKDLTYTVDV
                     EFQSNTADQRLFGMNVRFFYDDTQLEFISFNNFKDGYGSMSSNPPSINKGSNTSGVQL
                     FGFSDAATYVNGAVQLLNSSSLPVYISTSDWTMVYSVSFKVVDLELFYTGDFSPSLIW
                     DLQEDPSNGSFLQGSSGVVITVVAPPPLESTYSDETAVQFNWQYNGNTSAPFGFPVVT
                     NSISPCLIMVANNDDFSGNVISSFEGGVAGNVLVNDLVNGQPPVAGQISLSIVDEGGI
                     NGVSLSYDGKLSIPPVTLGGNYSVVYRLSDYEDSDNYKDATVLVSVADPEFKCPADLV
                     VCFNQGPVPLEGAVPGGGIYSGNGIINSVFDPSVAGIGTHFLSYCIENPFTHVILCCD
                     FTIQVTDGQLVSLSEGWNGISSYIMPHDPSMRFVLSPVDQSLGLIYNFEGIYWPEMGI
                     TTLNDWNPYSGYITKSINDVVLPVCGSPLSNSSLSMVSGWNLIPVLSAEPYNVEDLFS
                     SLDITVAKDIAGTGVYWPAYQINTIGYFQPGKAYMVRLNSSGIADFGMQQKSSSLSTP
                     EDINLLNTPWNAVTFTPETHLVAFNLTDNPLKTGDIVGGFTALGLCSGVTEVGNISTP
                     FALTLFMDDIYTGDTDGFKDGEPISFVVYRPSTGELFDMLVSYNPSMNQGYFESNGLS
                     EVNNLKLSSTGMIIIDTDQLQVFPNPTHGIFTLDGLAGNANIKVYNAYGKEVVKATTL
                     LPHTFDISGIARGIYMIRISVDQKMYFRKLIVN"
     gene            complement(8288..9139)
                     /gene="folP"
                     /locus_tag="PK345_00795"
     CDS             complement(8288..9139)
                     /gene="folP"
                     /locus_tag="PK345_00795"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HPX33590.1"
                     /translation="MNFKQILFRYNRTLEFSKPTIMGIINLTDDSFFDGGRFTNDQEV
                     IDYAGKMISQGASILDIGAMSTKPFSTEIDSNEEWERLEKIIATLRKKFPETIISIDT
                     YRAEVAKRSIAAGADIINDISGGEMDKGMISLIAKLNVPYILTHMQGTPKNMQQNPHY
                     NNIFEEIFTFFSVKLKQLKDLGYSSTTILDPGFGFGKTVEHNYFLLNSLKKFKELNCP
                     ILVGVSRKSMINKVLNTTPAEALNGTSVLNTISLLNGADILRVHDVAQAREAVELVGF
                     YNKCQNL"
     gene            9241..9891
                     /locus_tag="PK345_00800"
     CDS             9241..9891
                     /locus_tag="PK345_00800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33591.1"
                     /translation="MKFKLLTLNSFVLLIGLIFSDGIIAQQIEIKPVNREDQQLQRAT
                     DKVTRAEASVTKANQEIKMADSLESAGKLQISTAKEELSTAKADKSRLQKEYSTERKN
                     LERQLKSADRKQQEKIKQDIRELDSKHREQTREADNRIKNAGRSYDKGKTNVTKGQAK
                     KKEANKKLKTAEKSLSEANKELNKISPQEQPVKKLNIEGKPKKKTLKSDEASDEQK"
     gene            9932..11209
                     /locus_tag="PK345_00805"
     CDS             9932..11209
                     /locus_tag="PK345_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33592.1"
                     /translation="MKLKTMKSIMGWVIILLTIFSAVLIYFFQQFNILFLALLLFEII
                     LVIRYRKIFSPLMIRVSQILIGLLFLFSGFSKAVDPLGTAYRIGDYLTAYHMKNDFGI
                     TIFLSFVLNTAELLLGGLLLLNIKPKITPWLVALMMGIFTLTTWYDAIYSPVPDCGCF
                     GDAVIMTNWQTYYKNLAINVFVLIVIFGRLKIKSFFGNIIEWGIALLLAVIFVGFQYS
                     NYIDLPMLDFRPYKIGNRIYPENLMPVKTYLTYRNKNTGETKEYLSPNYPFDDQEWVD
                     NWEFVDMRVEDPNKMDGINLAIIDLDGEDITQTIVQNPDYQFFIVAWDLESADLGAFD
                     KINQWSVAAEEIGIRLIVLTSSEEKLINDLIDERNLTDAFEFYNSDDIELKTMIRANP
                     GLMLIKNGLIINKWHNNKIPDWKTLSRDYFLNH"
     gene            11444..11680
                     /locus_tag="PK345_00810"
     CDS             11444..11680
                     /locus_tag="PK345_00810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33593.1"
                     /translation="MSLDRQIDNKAPNYLKQALGCPCHAPYADNAGIQLALGQVFERD
                     KDHLFNYYRDMLIAIYAGLAVEEILLNGLSKALD"
     gene            11744..12571
                     /locus_tag="PK345_00815"
     CDS             11744..12571
                     /locus_tag="PK345_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HPX33594.1"
                     /translation="MICFINNSFVPYRDATLHISDVGLQRGFGIFDYFISVNGAIPFF
                     DDYLNRFYLSAEKLNLKVPFDRGDLKEKIYHLLNENKLAITGIKLLLTGGFADDLYTP
                     TDPNFLIINIPVPNYSDTYSDGIKLLLLDYVRYLPEVKTTFYLPALSLLPELKRIGAL
                     ETLYHHKGIVYEATRANIFIIKNGVLITPSEGILKGITRKHVLKVARLQMKVEERIVK
                     LDEIFDSDEIFITGTSKHILPVVNIDGKIIANGRPGTITNKLAQDFKAYYQDQIDKK"
     gene            12600..12731
                     /locus_tag="PK345_00820"
     CDS             12600..12731
                     /locus_tag="PK345_00820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33595.1"
                     /translation="MTKKYSIVLLQVKHQKIVMTIKYYYQHDYYSNTSKEISDQLFM"
     gene            complement(12802..13485)
                     /locus_tag="PK345_00825"
     CDS             complement(12802..13485)
                     /locus_tag="PK345_00825"
                     /inference="COORDINATES: protein motif:HMM:NF027590.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4918 family protein"
                     /protein_id="HPX33596.1"
                     /translation="MKTFGKQAIDFFQQVELNINIQGIEVMNPYKKPEIFNLTATFFK
                     KFYDDHHSRTFIFGINPGRFGAGQTGIAFTDPINLAEECKIQNNLPQRHELSSQFIYK
                     VITEYGGVEQFYKKFYITSLSPLGFTKDGKNLNYYDLQILKEAVYPFIVESTRKQFYF
                     GANPHKAICVGGDKNFKFLTQLNSSLKLFKKIIPLEHPRYIMQYKRREIDWFSKKYLN
                     TLKNCELNT"
     gene            13588..15702
                     /locus_tag="PK345_00830"
     CDS             13588..15702
                     /locus_tag="PK345_00830"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPX33597.1"
                     /translation="MKKISMLLMTILVIFFVSCKQQSKHAKDMNPFLMQYNTPFNVPP
                     FDKINNEHYLPAIKEGIEQQKREIEDIINNEKPSDFKNTIEALDYSGYLLTEVLSVFG
                     NMQSAHTNDDLQKIAQEASPLLSQHSDDIVLNEELFARIKSVYDNQEELGLNTEELKL
                     LQKTYKSFVRNGALLNEEEKAELREINSELSLLSLKFGENLLAENNNFQLIIDDEADL
                     DGLPQSVRDAASEAAQEAGMSESWLITLHNPSRVPFLQYANNRTLREKVMKAYIQRGN
                     NNNVNDNKAVVERITSLRVKRANLLGYKTHADFVLEETMAKTPEAVYSFLHDLWEPAL
                     NNAKKEASELQKMIYDSGGDFKLESWDWQYYAEKLRQQKYDFDEEALRPYFELKSVLN
                     GLFDVVNKLFGIKFVPLQDVPLYHPDAVVFEVTEADGAHIGILYMDFYPRASKTGGAW
                     MTSFREQFKKNGENIPPVISIVTNFTRPTGDQPALLSFDEVETLFHEFGHALHGLLSD
                     VTYPKLSGTNVPRDFVELPSQIMENWAEHPDVLQSFAKHYKTGETIPVELIQKIKNAS
                     KFNQGFATTEYIAASLLDMDWHTLTDTIPQKTNLFEEESMSRAGLIHEIDPRYRTTYF
                     AHAFSWGYSAGYYSYLWAEVLDNDAFEVFLENGLFDPATSKSLRENILSKGGTEDPME
                     LFIRFRGQEPSKTPMLRNRGLL"
     gene            15771..17678
                     /gene="cysN"
                     /locus_tag="PK345_00835"
     CDS             15771..17678
                     /gene="cysN"
                     /locus_tag="PK345_00835"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: protein motif:HMM:NF004035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HPX33598.1"
                     /translation="MDIHEFLDQDQQKDLLRFLTAGSVDDGKSTLIGRLLFDSKKLYE
                     DHLQALERDSKRIGHAGENIDYALLLDGLKAEREQGITIDVAYRYFSTNKRKFIIADT
                     PGHEQYTRNMVTGASTANLAIILIDARKGVITQTKRHTFIVSLLGIRHLVLAVNKMDL
                     VNYSQEVFEKICSDYKRFITGLDIPDVNFIPLSALNGDNVVELSLRMPWYHGESLLEF
                     LENVHVGSDHNFSELRYPVQFVLRPSLHFRGFSARIASGVIRKGDTIRVLPSGKESVV
                     TSIITANGESEFAFPPQSVTITLKDEIDISRGDMIVHPDNLPMVNRHMEAMLVWMDEK
                     PMKPETQYYIKLNNNTTKVKIENILYKIDVNTLEKSNIEFFTLNDIGRVIITTNKPLF
                     FDRYKHSHSTGSFVLIDPMTNNTSAVGMIIDEVKGKGSFSRITDIDRNKIERGECLVP
                     LDQRERFYDQKGVTLWITGLHGSGKNTLAYLLEKRLFEEGATTILLDGSTIRSGLSRE
                     LDQLEAHRAEHLRKVAQVARILNDQGVIVICSFISPDEEIRKQVAEIIGKERFLLTYM
                     NADLEHSRNFRPELYDKADAGLVYDVPGYDAKYEIPPNPDFNFKPNEMETNCDTIMDY
                     FRESGVFPLKR"
     gene            17797..18138
                     /locus_tag="PK345_00840"
     CDS             17797..18138
                     /locus_tag="PK345_00840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33599.1"
                     /translation="MQLLSLSQQWGLPFQNPVLIFSLILFIILFAPIILNRFKIPHFI
                     GFTSYHNNMFKIPDYLNKYFTENNYFLVYPIQSGVGDDVSLTLSNPSVMEPFREIEEL
                     RKIITKLFKNK"
     gene            18173..18715
                     /locus_tag="PK345_00845"
     CDS             18173..18715
                     /locus_tag="PK345_00845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33600.1"
                     /translation="MKKFISIMLLMLLIAPPVVTFSWLHMKKRMLRKEIKWKIINGID
                     KEELVLLTFSNDQAEACLKWHHSAEFEFMGEMYDVVETIFFDDSVSYYCWWDHEETLL
                     NKKLKAMLALSAGKSKQERQQINYIIQFFQSIFFIENQFGSNSMIFNNTRLDITYFFV
                     VKTAGIIPPVPPPRLNSRFI"
     gene            18944..21358
                     /locus_tag="PK345_00850"
     CDS             18944..21358
                     /locus_tag="PK345_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPX33601.1"
                     /translation="MKKILFFVVIIIITQANVFGQKLTIKDKDSGRPLNLVSLSSANP
                     LASATTNTRGQVDISDFKGSDAIEFRLLGYQGITLSYNALKELDFTIALSQSLFSLDE
                     IVVSATRWEQNKREVPNKITSINVKQIELQNPQTAADMLGNTGEVFIQKSQLGGGSPM
                     IRGFATNRLLYSIDGVRMNTAIFRAGNIQNVISVDPFSIETSEVFFGPGSNIYGSDAI
                     GGVMIFRTLTPQLSENKDPLVKGKALARYSSANNEITGHFDVGIGWQKWAFTSSVTNF
                     NFGNLMQGSRGPDDYLKRYYVKRVDSVDRVFVNENPRLQTPSGYTQTNIMQKVRFKPD
                     EKWDFNYGFHYSETSEYARYDRMAELSKDGLPNSAVWNYGPQKWMMNHLEITHSGPNP
                     VYDQLVIRVAYQKFEESRIDRKFNNNRLRTQLEEVEALSFNADFLKRSGKNNFFYGIE
                     AVVNDVVSTGSAVNIKTGKPVDVPDRYPDSKWKTYAAYINYQYRLSQQLLLQVGGRYS
                     QYFINSDFDKLLAFYPFDYKEVEVNKGSPTGSVGAVYTPTDKWTLSTSLSTGFRAPNV
                     DDIGKMYDFKAGDVIVPNPDLKAEYAYNGEINITKVFGSSVKFDITGFYTYLDNAMVR
                     REYTVNGQDSIIYNGEMSKVFALQNAADAFVYGINAGLEIKLPEGFGISGRYNYQFGE
                     EEMEDGTTSRSRHSAPSFGYAKLTYNANRLDMQFSAVFNGKIDHKDMNIEEQQKTSIY
                     AADKDGNPYVPSWTILNFKAMYRLTDMWTISAGLENITDRRYRTYSSGISAPGRNMVL
                     SLIANF"
     gene            complement(21461..22729)
                     /locus_tag="PK345_00855"
     CDS             complement(21461..22729)
                     /locus_tag="PK345_00855"
                     /inference="COORDINATES: protein motif:HMM:NF026018.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HPX33602.1"
                     /translation="MLNKILNTFGTRLLSAMLNLLITILISQFLGPEGKGEQGIIIAT
                     IAYILVFVNLMGGSAIVYLVPRFPVSVIILPAYLWTVLTSILFYGFLQLSTLVSEGMI
                     IHVCILSAISAFTTINSSVLIGKEKIAISNLVNFIQPLIITLLLIVFFFYLGKNTIES
                     YIISLYISFSAGFIVSLIYLKRFTDKFELADISSYKTIISNLFRYGLLNQLAHIFQLL
                     SFRMSYYWLEQTYSSAEVGIYSNGTSLVESIWLISRSICLVQYARIANSADLEYSQKL
                     TLQLNWASLIISFIGLLFMVMLPSRFFIFLYGEGFGEVSSVIRALAPGVLFFNTALII
                     GHYFSGIGKYYVNTISSFAGLLFAVLFFRLLIPVYGITGAGWATTISHLITSLTVVWF
                     FSRESKIKLKNSFPSMTDIKLIFSTLRVKK"
     gene            complement(22816..24009)
                     /locus_tag="PK345_00860"
     CDS             complement(22816..24009)
                     /locus_tag="PK345_00860"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPX33603.1"
                     /translation="MKKDFTNKRLSVLYLPRWYPHRYDPMMGLFIERHGLSVLPHADI
                     SVLYVHDDEKLTKRSYEIVTSKEGLFTVRVYFKKSMIKPRFLANLINLYRFFRSHISG
                     FKIIKNEYSKPDLLHVHVLTRCGIIALIYKIFTGVPYAITEHWTRYLPNTDSYYGGLR
                     KWLTRIVVKHAGAVMPVTANLRDAMIAKGLKNKNYIVIPNVVDVEKFKPAPKVYDPNK
                     KSLVHLSCFTDKQKNISGILRIMKKLSEKRDDFFLKLIGDGEDFIAMKSMSDELGLTG
                     KFVEFTGLKEGEALVELLNQADLMIMFSNYENLPVVILESYSCGVPVISTRVGGIHEH
                     LNDTLGRLTEPGNEDQFLEAIEEFLDNPKEYQSAKIREYALNNFSNEIIGNSIYNVYI
                     ELIAS"
     gene            complement(24036..24557)
                     /locus_tag="PK345_00865"
     CDS             complement(24036..24557)
                     /locus_tag="PK345_00865"
                     /inference="COORDINATES: protein motif:HMM:NF024911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX33604.1"
                     /translation="MSKLIKIAITGPESTGKSLISKQLASHYNSVWVPEFARAYLLNK
                     NEPYNYDDILEIAKGQISSEKAISTLANRILFSDTELIVTKIWCDVKYGKCHSWIEKQ
                     LIRQKYNLYLLMDIDLPWEFDPLREHPDRREFLMKKYIEELDRHGFNYSIVNGIGETR
                     LQNAINYINQLIK"
     gene            complement(24520..25167)
                     /gene="pnuC"
                     /locus_tag="PK345_00870"
     CDS             complement(24520..25167)
                     /gene="pnuC"
                     /locus_tag="PK345_00870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015663 - nicotinamide mononucleotide
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015890 - nicotinamide mononucleotide
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HPX33605.1"
                     /translation="MLTTTFFDLFYQNLLQSTWLEGVAVIFGLLSVWYAKKANILVYP
                     TGIISVLIYVYICYHAGIYADMGINFFYFLVSVFGWYKWTRKDSSNKELAISRCSLLH
                     HFIWVLLTIISFLILQYILRHYTDSTIPYIDSFTTAIFITGMGLMALKKVENWIYWII
                     GDLVSVPLYFHKELVLTSFQYFVFLILAVLGYIEWKRILKQSEVEQTHQNSHNRA"
     gene            25542..25615
                     /locus_tag="PK345_00875"
     tRNA            25542..25615
                     /locus_tag="PK345_00875"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25576..25578,aa:Arg,seq:tct)
     gene            complement(25701..26621)
                     /locus_tag="PK345_00880"
     CDS             complement(25701..26621)
                     /locus_tag="PK345_00880"
                     /inference="COORDINATES: protein motif:HMM:NF027445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4835 family protein"
                     /protein_id="HPX33606.1"
                     /translation="MKYPNIILSFCSIILLAFIFNKVQAQEFFCSVQISAPQVQQTNR
                     EKFNELRQGLYEFVNERRWTNYTLTRNERIEATIAITITEEIGSDEFKGKMNLVLKRP
                     VYGTSYSTTMFNYQDPNFQFQWQPGQAIEFEDNRFTSNLTSTIAFYIYIFLGLDFDSF
                     QLMGGTPYFEKAQQIVNSAQSAKEPGWKSFESIRNRYWIAENLTNSSYANVRKFNYQF
                     HRLGLDLMQDNMEKGRQGVTLALETLQRAYREKPNLYIFQLIMDSKRDEFINIYSQAA
                     GMDKTKAVNMLKEIDPSKAAQYDRIMKQGP"
     gene            complement(26625..27839)
                     /gene="coaBC"
                     /locus_tag="PK345_00885"
     CDS             complement(26625..27839)
                     /gene="coaBC"
                     /locus_tag="PK345_00885"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005678126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015939 - pantothenate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HPX33607.1"
                     /translation="MLSGKKILIGITGSIAAYKIPFLIRLLVKEGAEVQVLLTENARD
                     FVTPLTISTLSKHPALTYPFNSADGSWTNHVELGNWADLMLIAPTTAATLSKMANGLA
                     DNLLMATYLSAKCPVFFAPAMDLDMYNHPSTKKNIEQLKAFGHRLIEPQVGELASGLT
                     GAGRLEEPENILKIINAFFLHNNELTGKRILVTAGPTYEPIDPVRFIGNHSSGLMGFS
                     IAEECANRGATVTLISGPTHLKSDNKAIRCIDVLSANEMYEQTIKEFAHVDIVFMSAA
                     VADFTPSHPSDKKIKKESGTPVIELKPTKDILLELGKLKQKNQILIGFALETDNEIEN
                     AKTKLNTKNLDLIVLNSLKDEGAGFGYPTNKVTLINNSGKKWEYGLKTKKEVASDILD
                     VVTTMIKKGQAF"
     gene            complement(27839..28192)
                     /locus_tag="PK345_00890"
     CDS             complement(27839..28192)
                     /locus_tag="PK345_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HPX33608.1"
                     /translation="MDYKKIKIEPTAVTRRVRDFSKGTNNIYETIVILSKRANQISIE
                     MRDELKKRTEEFALTTTNLEEIFENREQIEISKFYERLPKPSLIAIHEFLNDEIYYRN
                     PSKELEKKSTKDYNL"
     gene            complement(28214..29023)
                     /gene="bamD"
                     /locus_tag="PK345_00895"
     CDS             complement(28214..29023)
                     /gene="bamD"
                     /locus_tag="PK345_00895"
                     /inference="COORDINATES: protein motif:HMM:TIGR03302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0065003 - protein-containing complex
                     assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HPX33609.1"
                     /translation="MFKKRIFISIILISSILLSCSKHQKLLKSTDNEKKYEIAMQYYD
                     KGDFYRALQLFDQLIPIYRGTSKSEDLLFRYPFAYFNQRDYVMASYHFNRFSSTFPLS
                     SKAEEAAYMAAYCKYLESPRHTLDQTVTKDAINEFQSFINRYPYGEKAKEATKLIDQL
                     RLKLQKKDYNIANLYMKIGDYQAAIVSFRNLLKDYPEIAYKEEIMYKIVVSAYNFANN
                     SIQEKKQERLESAQNDYYDFITLFPESRYVNELNKYYKRSIDQPKKGSDNI"
     gene            complement(29170..30894)
                     /locus_tag="PK345_00900"
     CDS             complement(29170..30894)
                     /locus_tag="PK345_00900"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPX33610.1"
                     /translation="MEIIKKLKSLWKQLYGNAYEHELSNFIHYIKNFKNNLNISPQPS
                     EWYKDAIVYSLYVDLFNENFVGLRQRLDYIQKLGVNCLWLLPVLDSPMRDAGFDIRNY
                     KKIREELLGLSPEASEEERMTVFKEFIEDAHQRNIRIIFDIAMNHTSEEHPWFVEAKK
                     GKSNPFRDFYIWSANTDLYKDARIIFKGMETSNWEPADDEYFFHRFFSFQPDLNYRNP
                     QVLIEMAKNLLFWLEKGVDGFRADAIPYLWKEDGTNCENLPQTHTIVKFFRATLDFVQ
                     PHTLLLAEACQKPIEVVKYFGDGDECNAGYHFPLMPQIYKALALENSEPILYTLSKQV
                     TPEIPDNAQWFTFLRCHDELSLELVYVTEQDRALIHQNYCHDSQWDFRKGEGISARLA
                     ELMKRNPDKIALTYSIMLTLPGTPIIYYGDEYGKLNDQAYYEEQIRKTKKDDTRFLVR
                     GKIDWETLENNLENPNSFASQVFHKISKMVNIRKDIKVLGRGQIEWLEALKPDGTKNN
                     SLLVYIRKTNEERFLIIQNLSSSKQEGRLALQPSSTWKELFDNPLDFDQNLQTFTLKP
                     FEYVWIRM"
     gene            complement(30917..33556)
                     /locus_tag="PK345_00905"
     CDS             complement(30917..33556)
                     /locus_tag="PK345_00905"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPX33611.1"
                     /translation="MRRVPVLLFPPKRDYVPYLAIDIILFSSDKIQIITYCAILYFCT
                     EIKNRQTFIRVNKKIVIPLIILGILASAFFVANYLTNCSLFQKEKKNQYISHHLSDNK
                     LVIKTSLGSISVSFINSSIVQVSFAQKKSDQEVTSHSVVLSADKTGGALFEDENELFY
                     TRDKLQVVIKKSPFKLIFFQKGNQILAESDGYFKSNEVEGFRFSLKPDEKIYGAGFRT
                     TPLNRRGHRYELYNQAVYSYNLNSPNLNFSVPFIISSKGYGLLFDNASKGWLDLDVHQ
                     NNVMEFSSIGGEMSYYVIADNDFDSILKEYGSLTGYQPMPPRWALGNLQSKFGYKTQH
                     ETEAIVDQMIEAGYPLDAIIIDLYWFGLGFHDHFYMGNLDWYKKNWPEPEQMIQRFKQ
                     RGIKTILITEPFILQESKNWQLASDNKYMGTDNNGNTFVIDDLWFGPGGLIDVFNPDA
                     RRWFWEQYRRQIEIGIAGWWGDLGEPEKHPAKMQHFIGSAEVVHNLYSHYWHKMLWDY
                     YSLEYPEVRLFNLNRSGFAGSQRYSVYPWSGDVSRDWNGFQVQPLAVLGMTLSGFSYM
                     HSDLGGFAMGEPDEELYLRWLQYGAFNPVFRPHGDTNAPVEPIAYSDNAQKIIKEYID
                     LRYRMLPYNYSIAWLNSIKGTPLTKPLFFEEPDNDEISNIDDTFLWGPNLLIAPILEK
                     DAKMRKVYLPKGKWYDFFTDQVMNGGKWIEKSTTLENIPVFARGGGFIPMINPIKNTT
                     AYTSENLIIHHYYDPEIINSEFIIYDDDGETKSAYEKGLYELLEIKSMHFDSFVLFTF
                     NRKEAFNYNGKPATRNIELLIHGYNSKPGKIQTGRVPVKNALSKQAYQTTKTQISFWD
                     SEKELLQIKFQLSESITNLQIIF"
     gene            33730..34365
                     /locus_tag="PK345_00910"
     CDS             33730..34365
                     /locus_tag="PK345_00910"
                     /inference="COORDINATES: protein motif:HMM:NF020463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WbqC family protein"
                     /protein_id="HPX33612.1"
                     /translation="MSKQFSVLLSTAFLPPIQWFVYLLKAEKVLIENFETYPKQTYRN
                     RCEIATANGKQVLTIPVIKIFGNHTKTNEIEIANDEKWQIRHWRSLVAAYANSPYFLY
                     YQDELQPFFLEEYINLMEFNLCIIKSLMAIIGIYKSIELTDTFELNPKNVFDLRNEIT
                     PKRTFDHFHLPTYYQVFEEKNGFFSGLSIIDLLFNMGPETFDVLNSVNVNC"
     gene            complement(34393..36465)
                     /locus_tag="PK345_00915"
     CDS             complement(34393..36465)
                     /locus_tag="PK345_00915"
                     /inference="COORDINATES: protein motif:HMM:NF019317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPX33613.1"
                     /translation="MNQFWSKITQNYSTIQKTFLFLISIAILVTFFPKEAKFKYDYTR
                     DRPWLHNDLIAPFDLGILKPADELEKEKQEIIQNNELFFEFDLAVYENKRSEFSKNLD
                     KAWVSKYGSINVKENLKRSTSQTGLDILSLIYTRGVLQSVSEIDNKSDDHNIMILIDN
                     TAERAKLSDFYTIHNAYSEMLKKVEEAEFADKELLIKTLEEAITQNVLFNQRVTLAEV
                     ENKLNNISTTRGMVQEGERIISKGEVVTLAKYQILESLKKEYQDRASDNSNFRLILLG
                     QVILVSVSMLVLILFLYVFRKEIFEDTKKIVLILLTIILMVFVTSLTMKFGEGYIYII
                     PLCIVPIIISVFFDTRVALYVFIITIILIGFLVPNSFQFVFTQLIVGIITIFSIINLT
                     RRSQFLVTSLIIFVSYSVIYTGLNLLQQGNFDEIRLINYAMFGGSALLILLSYPIIYL
                     FEKLFGLVTDVTLLELSDTNSKLLRKLSMKAPGTFQHSLQVANLAEECIQIIGGNALL
                     TRTGALYHDIGKMDNPRYFIENQLTGVNPHDELTYEESASIIIEHVTKGIEEAKKNNI
                     PDQIIDFIRTHHGTRKVDYFYIMQKRENPDETIDERLFSYPGHVPFSKETSVVMMADS
                     VEAASRSLKVVDEKSISTLVDKIIDKQVESGQFSNSNITLRDITQIRKILKRKLMNIY
                     HLRIEYPG"
     gene            36651..37796
                     /locus_tag="PK345_00920"
     CDS             36651..37796
                     /locus_tag="PK345_00920"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPX33614.1"
                     /translation="MKIAVNTRLFIKGRLEGIGWFSYECLKRITTQHPEHQFYFIFDR
                     KFDEEFIFADNIEPIVAFPQARHPFLYYLWFEHSIPRVLKRVGADLFFSPDGYLSLCS
                     KTPSVNVFHDLNFEHYPEDLPYFERKFYRHYFPKYAHKALRIATVSEYSKNDIVSLYG
                     VDNDKIDVVYNGANESFGPISEPEKQVVRERFAHGAPYFLFVGSLHPRKNLARLFPAF
                     DQFKISDNDSVKLVIVGEKKWWTEAIEKAYNQMNYKDDVIFTGRLPVKDLHQVIASAL
                     AITYVSYFEGFGIPILEAFYCHTPVITSQVTSMPEVAGDAAILVDPMSISSIADGMAR
                     MANDENLRKSLVEKGIERSAMFSWQKSSERLWNTIQKALDTINQEAK"
     gene            37887..39257
                     /locus_tag="PK345_00925"
     CDS             37887..39257
                     /locus_tag="PK345_00925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HPX33615.1"
                     /translation="MEEKTKKADQQNSNKLEFNLDCTPTIDKVSEPKVLNYNLKKRTN
                     KRVDESWDFRNANTKEFTHCFHSYPAMMIPQVARRIINTYGSNAKMLFDPYCGTGTSL
                     VEACLAGKNAIGTDINPLARLIATTKTTKLDIQVLDLFFHDFMDYFFALNFQDDGIKS
                     MIIPEVKNIDFWFSKSVQRKLGILLGYIENINDISIRNFFKVAFSETVREVSYQKPGE
                     FKLIRRKDFDKRVEPDVIGIMISKLTRNRRGLIDFVKSYDNNSSISIYDFNTVENIPI
                     NILKDESVDIVVTSPPYGDSRTTVAYGQYSRLSNQWLGFLHANQIDNKLMGGKKSKQL
                     YEFKCDFLNGVIYAVQKQDSERVRDVVSFYKDYEKSIGLVSRTLKKGGFVCYVVGNRT
                     VKGIKIPTDEITTKLFEQNNFTHIETIVRNIPNKRMPSKNSPSNIAGKTASTMKNEYI
                     VICQKK"
     gene            39259..39960
                     /locus_tag="PK345_00930"
     CDS             39259..39960
                     /locus_tag="PK345_00930"
                     /inference="COORDINATES: protein motif:HMM:NF026853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MvaI/BcnI family restriction endonuclease"
                     /protein_id="HPX33616.1"
                     /translation="MQLKEIISRLKELKAKGFIPSPRKGPTGVGYCFEQELGVSESNI
                     PIPDVGGRVELKGTRKKTNSLITLFTFNRAVWIIKQKDLIDTYGYKDAKNRQALYSTV
                     NNKSPNAQGLYIELDMDKNLVILRHKDAEFSLAEWSIYVLAGKFMTKMDRIILGFADT
                     KEINGKEFFHFTDAVLLEHPTPEMFLEAFSKGEILLDLRMHLKESGAVRNHGTGFRIS
                     EKNLTMLYSKQKKLL"
     gene            complement(40034..>40468)
                     /locus_tag="PK345_00935"
     CDS             complement(40034..>40468)
                     /locus_tag="PK345_00935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005677274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPX33617.1"
                     /translation="MSYVAAQRTLYRYNDMADAKALLESIARSFGYIYGRERKVRVNT
                     VSQSPTKTTAGSAVKGFDGLMDFTERMSPLGNASADECADYVITLFSDLTKKVTMQNL
                     FHDGGFSSMGMSSRAMLMYNRSLDCKDCPDYINDEKDISTNQ"
BASE COUNT        11918 a         7220 c         8106 g        13224 t
ORIGIN      
        1 ggtgcaggag ttgttggagg cacagatttt aatcccgctt ttgctgacgt aggagagcat
       61 ttaattactt atgaagcaat tactaatagt ggtatagaat catgtacctt cactattact
      121 gtgcttgatt cacctgatgt tacctgtcca tctgatattt ctatttgtat taccactcct
      181 gaagttgtac tcactggagg gcatccttct ggtggtgttt attctggtac cggtgttgtc
      241 agcgatactt ttgttacggc agatgctggc gccggcgaac atcttattag ctatacctat
      301 gttgatgtta atggatgtga gaatgcttgt gattttgtaa ttaccgtcgt tgatgagccc
      361 tcagtagatg ccggccctga tataatcatt tgtggcgatg gccctgttga gatttgcggc
      421 gtggtttcca atacttcaaa ttttgtatgg caggttgtag gaggtaatgc tatgggtact
      481 ttcgacgatg attgttctat atgcaccacg tttacaccct cagaagcagc attagaacag
      541 gggttcgtta tcattagcct tgttgttttc ccgcagagtc cttgtacact acccagggta
      601 gatattatgg tcatcacttt ctcatctgct cctactgctt ttgcgggttc tgaagggtct
      661 atctgttatc ctgatttaac agagtgggca ttaactgatg ccagcgctga ccactatagt
      721 tctttattat ggagttcgac aggtaccggt tcttttgatg atccatcctc tttgacgccg
      781 atctatacgg taagtgcaga agatgttctt gttggtagtg taacacttac tttgacagca
      841 tatcctatcg agccatgtac agaccctgcg atcagtagtg ttgctcttta tattgcacct
      901 tctcccacag tatatgccgg tattgatacc attatttgtc agtttgagtg ctgtagtggt
      961 ggtgatgcag gcagctactg tctgagcgct gaagcagcca actattcatc gttactttgg
     1021 tcgagttcgg gagatggcac atttgccgat ccaagccagc tcaacacatg ttatgttatt
     1081 ggttctgatg atatggttgc cggcgtagtt acccttactt taacagcata tcctatcgag
     1141 ccttgtgccg aacctgtaca gagtcaaatt ttactgacta tccagcctgc gccaatagtt
     1201 gatgccggtc cagacgctac tatttgcgtt ggtcagactt atactttatc aggctcaagt
     1261 gccttatata gtagttcact ttattgggat tttgcctata tgggagaggg cgatggtgtt
     1321 tgggatgatc atactgtttt acatcctacc tatactcctg gtcctggcga tttagataga
     1381 ggctatgtta cattgattct cgtgggcttg gttacgatcc ttgttacagc gttattgatg
     1441 ttgacgttat gaaattatat tttgagagtg tacctgatgt atcatttgaa gcatatcctg
     1501 tttgttttgg cacagaggta tcctttatca atttgtcctc atccggtacc tggctatggg
     1561 attttggcga tggtagcaca tctgatttaa aagatccgac tcatctctat gacgctcccg
     1621 gtgtttattc tgtttgcctt actgtttctg tcaataccgg ttgttcagat acattttgcg
     1681 gcgaggtaga ggtttactcc ttaccagagg ttaatgccgg tagttatcca actttaacta
     1741 tttatgatga tattacctta caaccaatag tttcgggagc aacgcctctc caatttgagt
     1801 ggtctccatc cacttattta agtgatccac aagttgaaag accaattttt ggccctgctc
     1861 cccttggtgt ttatacttta acccttactg taacagatga taatggctgt tctgcacagg
     1921 ctgaaacgat tattgttgta gggccttcag agatcattgc caacgatgat attggtactc
     1981 ctgttaatgg ttacgatgga ggagttgttg tagctaatgt tctttcaaat gatttactta
     2041 atggttcagc tcctacgctt tcactagttg acctaacaat tgttacccca ccgagccatt
     2101 ccggtatagt tctcgatctg cttaccggtg aagttacagt agctcccggc actccagcgg
     2161 gtacttacac ctttacatat cagatttgcg aaattctcaa tcctaccaat tgtgacgatg
     2221 cccttgtaac cgttgttgtt actcctccag atattttagc tgtggatgat gacttcagtg
     2281 gcaatccgat caatggcatg gatggaggag ttgccggtaa tgttcttgat aacgatcttc
     2341 tcaacgatga tcctgtagat gcaggagata tcaccatttc agttgtttct gatggtggcc
     2401 ttaccggtgt tgttattgat tctgatggta atttgaatgt tcccccctct acacctgccg
     2461 gaacctatac cgttacctac gagatttgcg agattatcaa tccaactaat tgcgatcagg
     2521 ctgttatcac gattgtagtt actgcttcta ctattatcgc taacgatgat gttggagctc
     2581 ctgtcaatag tgatgatgga ggcgttgctg tagctaatgt tctttctaat gatttactaa
     2641 atggttcgga gcctacgctt tcacttgtta gccttacaat tgttactccg ccgagccatt
     2701 ctggtatagt tctcgatctg cttaccggtg aagttactgt agctgccggc acaccggtgg
     2761 gtatttacac ctttacctat cagatttgtg agattctcaa tcctaccaat tgtgatgatg
     2821 ccatggttac cgtagtggtg attgaagacc ctgcagtttt gttctgcttt aatgatgtta
     2881 cagctactac cggttcagag tttgcttatt gcgaaggtca gaccatcact gttacacttt
     2941 gcgatgtctt agcaggtttg gcgccatttg atatttgttg ggaagttaat ggctctccgt
     3001 attgtgatta tggagtcggt attgatgata tcttatttag cgatcagttg ccagcaggta
     3061 attatgagat tgttattacc tccataactg attctttagg cgttaatgtc atagatgttt
     3121 ccgtttacca tgctactgtt atcgtacatg ccgatccctc tgtcgaagcc ggtgaggatg
     3181 atgttgtctg tgcaggcagt tcttatgagt tgttaggctc tttagcttcc aattacagca
     3241 gtctactctg gagtggtggc gatggtgtct ttagtgatcc tactgctctt catcctactt
     3301 atactttagg cgctaacgac cttgcagtag gaagtgtaac actctgtctg agtgcagagc
     3361 cattggatcc ctgtactgta ccagcttctg actgtcttgt tcttactatt caagacacgc
     3421 ctaccgtatc tcttgatagt gatggagttg tttgcgaaga tgaattcttt gccttagaac
     3481 ctgttgtaag taactattcc gaagttcaat ggagcggtgg ggtggatggt ttcttcttag
     3541 atgagaatga tccgtttact tcctattacc ccggagaaga agatattgca gccggaagca
     3601 ttgaactttg tatagaggtt tatgctgtat ctccgtgcat aggttctgta acggcatgta
     3661 ttacattaaa tattgaagtt gctcctgttg ctaatgcagg tgttaatgat acgatctgcg
     3721 aaggtgatag ttatcatctt tcaaccgcta cagctaccaa ttatggtagc gtcgaatggc
     3781 ttagttccgg cgatggtact tttgatgata atactgtagt taatgctacc tatacccccg
     3841 gtgaaggtga tattgctaat ggttcagtaa tattgttgtt acatgcttat cctgagtctg
     3901 tttgtactac tgttgctgag agtgcaatgc ttctaactat tgttggttat ccaactcttg
     3961 atatggattc tgaagttaaa ttgacttgtg aagattacga tgttgctgca ggcgcctgga
     4021 taccatttca aattgatgtt gtagccagta attatggttc ggtattgtgg accactaccg
     4081 gcgatggtta ttttgatgat cctaccgaca taaattcagt ttattatgta ggtctgaatg
     4141 atatttggca gccctatttt gaactttgtc tcactatcga aggtgttgga gtgtgcaatg
     4201 cttcagtaag caaatgcgtt acagtagaga ttccgcagca gctcatttat tacgatcagg
     4261 atggctggtg gggcttgtct tcatatcttg atccgtacga tccacctgta cccactgttg
     4321 ttgatcctat cgtactgatt ccaggttcgc aacatcttat cactatggtt gatagagccg
     4381 gtaaatattt ctgggctgaa gacatcccac caaagaatca gttaggagat tggaagccta
     4441 taggttataa aatcaagatg aaaaatactc ctgcctgctt acctatttat ggtgattcct
     4501 tgactaatca gacctttaat atcagtggtt tatttacctt cttaccggtt cttacaaacg
     4561 ttcctgtggc tattaccgat ctttttgctg atcatcttaa taagatccgg atgatctacg
     4621 attggcccac tgcacgttta tggactgagt atgtttcaga ttttgatatt ttgcaaccag
     4681 gtaaagccta cttattggtg aacaaggtaa catccaatta ttcagttaca ttccctgatt
     4741 atgatccatc cgctcctcat cgctatccta ccttaaaaag tagcaactac ttcacatctc
     4801 cttggtctga tattgttaat acagccgttc cacatgttgt tgtcttcgac aaatctgttc
     4861 agtctaagct ttgtattgga gatgttatag gagctttcaa tcatacaggt atctgttatg
     4921 gatcagttga gtttaatggt gttggcgagt ttatgaagtt ggttgtaatg ggcgatgcgc
     4981 cattttcaag tggtactgta ggtttcaaag agtcggagat tatctacttc aagctttaca
     5041 gaccctccac aggtgagacc tttaacctaa gctttattta tgatcaggat ttccctgtta
     5101 ccgatgatca ttttaccgaa aacggtatcg cacgtgttgt tgatttcaag agcgaggcta
     5161 ttgttgttag cgacctggat ttaagcaatc atgttattgt ctatcctaat cctgcaagca
     5221 ctaaccttac tgttgaaagt agcaaaccca tcactaatgt cgttatgctt aacagtttag
     5281 gtcaacgggt tttaagttct gatatcatgg gggttacagg cgttgttgat ttaaatggcc
     5341 tttcacgcgg tatgtatgtt atgcatctat acttcgaaag cggccatcat accataaaac
     5401 gaatcattat tgagtaaact tatgatctga agatgagtaa ggggtttaaa aggcccttta
     5461 ctcactggca gatcaataaa ttaaaaaaat actattaaaa agtacaatta taacttacga
     5521 aattaataac ataagcgaag ttttaggcgc ttgccgttta acgtgctatt tattaatgtt
     5581 ttcggagtaa aactaacaat tatgaaaaag ttaatattta ttttttcttt gttcaccttt
     5641 gcaggctatc ttatggccgg ctctccggtt atcactgtcc gttttgccaa tcctgagctt
     5701 aattgtaaag atctgaccta tacggttgat gttgagtttc agtccaatac tgccgatcaa
     5761 cgtttgtttg ggatgaatgt tcgatttttc tacgatgaca cccaactcga gttcatttcg
     5821 tttaacaact ttaaggacgg ttatggttct atgtcgtcca atccgccttc tataaacaaa
     5881 ggttctaaca caagtggcgt tcagctattc ggtttttccg atgctgctac atacgttaat
     5941 ggcgctgttc aattgttgaa ttcctcttcg ttacccgttt atatttcaac tagcgactgg
     6001 acgatggttt atagtgtttc ctttaaagtg gttgatctgg aattatttta tactggcgat
     6061 ttcagtccaa gccttatctg ggatttacaa gaggatccgt ctaatggtag ttttttgcaa
     6121 ggcagttcgg gtgttgttat caccgttgtt gctccacctc ccttggaatc aacatactcc
     6181 gatgagactg ctgtccagtt taactggcaa tataatggta atacctctgc tccttttgga
     6241 tttcctgtag ttaccaacag tatttctccc tgtcttatca tggtagccaa caatgatgat
     6301 ttcagtggca atgtaatatc aagttttgaa ggcggtgtag ctggtaatgt cctggttaac
     6361 gatcttgtca atggtcaacc tcctgttgcc ggtcagatta gcctaagtat tgtagatgaa
     6421 ggcggtataa atggagtaag cttaagttat gatggcaagc tttctatacc gccggttact
     6481 ttgggtggta attatagtgt tgtttatcgc ttgagcgact atgaggatag tgataactat
     6541 aaagatgcca cagtgttagt ctctgtagcc gaccccgagt ttaaatgtcc tgccgatctt
     6601 gtagtctgtt ttaatcaggg tccagtacca ttagaaggcg ccgttccagg tggcggtatt
     6661 tattccggta acggtattat caatagtgtt ttcgatccat ccgtagccgg cataggtacg
     6721 cattttcttt cctattgcat agagaatcct ttcacccatg tcattttgtg ttgcgatttt
     6781 actatccagg ttaccgatgg tcaattagtc tctttatccg aaggctggaa tggtatatcg
     6841 agttatatca tgccccacga tccgtcaatg cgttttgtgt tgtctcctgt tgatcagtcc
     6901 ttaggattga tttataattt tgaaggcatc tactggcctg agatgggtat caccaccctt
     6961 aatgattgga atccttattc aggttacatt actaaatcca taaacgacgt cgttttgccg
     7021 gtttgtggta gtcctttatc aaatagttct ttatctatgg tgtcagggtg gaatttgata
     7081 ccggtgttat ccgccgaacc ctataatgta gaggatctct tttcaagctt agatatcaca
     7141 gtagccaaag atattgctgg caccggtgtt tattggcctg cctatcagat taatactata
     7201 ggttattttc aacctggtaa agcatatatg gtacgtttaa atagttcagg tattgccgat
     7261 tttggtatgc aacaaaagag ttcttcttta tccactcccg aagacattaa ccttctcaat
     7321 actccgtgga atgcagtaac ttttacgcct gagacccatt tagtagcctt caatttgaca
     7381 gataacccat tgaagacagg cgatattgta ggtggcttca ccgccttagg actttgttca
     7441 ggggtaacgg aggtaggtaa tattagtact ccctttgcac tcacattgtt tatggatgat
     7501 atttatacag gtgataccga tgggttcaaa gacggcgaac cgatctcttt tgtagtctat
     7561 cgtccatcta caggcgaact gttcgacatg ttggtaagct ataatccttc aatgaatcag
     7621 gggtattttg agagtaatgg tttgtcggag gttaataact taaagttatc ctctaccgga
     7681 atgatcatta ttgatactga ccagttacag gtcttcccca atcctacgca tggtattttt
     7741 acattagatg gtcttgctgg gaatgcaaac attaaggtat ataatgctta tggtaaagaa
     7801 gttgtaaaag ctacaacact tttacctcat actttcgaca tatccggtat cgcccgaggt
     7861 atttacatga tcaggatcag tgttgatcaa aagatgtact tccgtaagtt gattgttaac
     7921 taagtaagta gctggtctta caacggcatt tatcaaatgt atatgctttc taataatgta
     7981 aaacagaaaa tgatgcttac ccaataagag gtttggataa atcaatatgg gttaaagaat
     8041 agtttttttt agaaaacata gttttaattt ttaaatgagt gagggttatt ttgccctcac
     8101 ttttttttgt gtgtttacga gtattatgat caccttttcc tgttttgtac aatgtgcgac
     8161 ccccttttaa aatttcattt ccaagcatat agcgttaaat caggtatatc aatcaagaag
     8221 cttgtagatg agactaaaat tacttttcgg atcggacaca atagattata aatttggtaa
     8281 tcaaggttta tagattttgg catttattgt aaaaacctac tagttctact gcttcacgcg
     8341 cttgggctac atcgtgtacc ctaagaatat cggcgccgtt aagtaaactg atagtattta
     8401 aaacactcgt accatttaac gcctctgcag gcgtggtatt taaaacctta tttatcattg
     8461 actttcttga tacgccgacc aaaatagggc aattcagctc tttaaacttt tttagagaat
     8521 ttaataggaa ataattgtgc tctaccgttt tgccgaagcc aaaaccaggg tcgagaatag
     8581 ttgtggatga gtaacctaaa tcctttagtt gttttaactt aacagaaaaa aaagtaaata
     8641 tctcttcaaa aatgttgtta taatgcggat tctgctgcat atttttcggt gttccttgca
     8701 tgtgcgtcaa tatgtagggg acattgagct tcgcaattag tgaaatcatc cctttgtcca
     8761 tttcgcctcc cgaaatatca ttgatgatat cggctcctgc agcaatagat cttttagcta
     8821 cttctgcacg gtatgtgtca atagagatga tagtctcggg aaacttcttt cgtagtgtgg
     8881 caattatttt ttcaagcctt tcccattctt cgtttgaatc aatttctgta gaaaatggtt
     8941 tggttgacat tgcaccaata tctaatattg acgccccttg gctaatcatt ttgcctgcgt
     9001 aatcaataac ctcctgatca ttagtaaatc ttcctccatc aaaaaatgaa tcatcggtaa
     9061 ggttgattat tcccatgata gtcggcttag aaaactcaag cgtccggttg tagcgaaaaa
     9121 gtatttgttt aaagttcatt ttatatttaa aagtaatttt ttacgtttcg ttaacatgct
     9181 gctaaaataa agcaaattaa ttttgtagaa atttttatta ctaatcaaaa ataaataaca
     9241 atgaagttta aattattaac tcttaactca tttgttttgc tgataggttt gattttttca
     9301 gatggaataa tagctcagca gatagaaata aaacctgtaa accgtgaaga tcagcaacta
     9361 caacgcgcca ctgacaaagt aacacgtgca gaggcatcag taacaaaagc aaatcaggaa
     9421 atcaaaatgg cagatagtct tgaatcggct ggcaagctgc agatttcaac agcaaaagag
     9481 gaactttcaa cagcaaaagc agataagtca agactccaaa aagagtattc tactgagaga
     9541 aaaaatctgg aaaggcaatt gaaatccgct gatagaaagc aacaggaaaa aataaagcag
     9601 gatattagag aattagattc taaacatcgc gagcagacac gtgaagctga caacagaatt
     9661 aaaaatgccg gaagatcgta tgataaaggt aaaacaaatg ttactaaagg gcaggcaaag
     9721 aaaaaagagg ccaataagaa gctaaaaact gctgaaaaat cactatccga agcaaataaa
     9781 gaacttaata agattagccc acaggagcag ccggtcaaaa aattaaacat tgaaggcaaa
     9841 ccaaagaaaa agacattaaa atcagatgaa gcatcggacg aacaaaaata ataatagaga
     9901 agcttaaaat atttttccca acttttcaga tatgaagtta aaaacaatga aatccatcat
     9961 gggatgggtt atcatcctcc ttacaatttt ttcggcagtt ttaatatatt tttttcaaca
    10021 gtttaatatt ctgtttttag cgcttctact ttttgagatc atacttgtga tccgttaccg
    10081 gaagattttt tcaccattga tgatcagggt aagtcagatt ctgataggat tgttgttttt
    10141 gttttctgga ttttcaaaag cggtggatcc gttagggact gcttatcgga ttggagatta
    10201 tttgactgca taccatatga aaaatgattt cggtattacc atttttttat catttgtact
    10261 aaatacagcc gagcttcttt taggcggatt gttgttgctc aatataaagc ccaagataac
    10321 accctggtta gtagcgctga tgatgggaat ctttaccctt actacctggt atgatgctat
    10381 ttatagtcct gtgccagatt gtggatgttt tggtgatgca gtgataatga ccaactggca
    10441 aacttattac aaaaacttag caattaatgt gtttgttcta atagtgatct tcgggaggct
    10501 aaaaataaaa tcattctttg gcaatattat agagtggggg atagcgcttc tgttagcggt
    10561 aatttttgta gggtttcagt attcaaatta tattgatctg cccatgctcg actttcggcc
    10621 atataagata ggtaatcgta tttacccgga aaatcttatg ccagttaaaa cctatcttac
    10681 atatcgcaat aaaaataccg gtgaaacaaa agagtatctt tctcccaact atccttttga
    10741 tgatcaggag tgggttgata actgggaatt tgttgatatg cgtgttgaag accctaacaa
    10801 aatggatggg ataaaccttg caatcattga tctggatgga gaagacatca cccaaacgat
    10861 tgttcagaat ccagattatc agttttttat tgtagcgtgg gatttagaga gtgccgactt
    10921 gggcgcattt gataagatta atcaatggag cgttgcagcc gaagagatag ggattagatt
    10981 aattgttctg acaagctcag aagagaaatt aataaacgat ctgatagatg aaagaaatct
    11041 tacagatgca tttgaatttt ataattcaga tgatattgaa ctgaaaacga tgatacgggc
    11101 aaatccgggt ttgatgctca ttaaaaatgg tctgatcatc aataagtggc ataataacaa
    11161 aatacctgat tggaaaactt tgagcagaga ttattttcta aatcattaac aaagagttaa
    11221 tgctctaaaa tagtgatttt tactttgtga agtaaagttg cggaataggt gatttaagta
    11281 gcctatacag atacctggac aaactatgcc ggttggcgtt aaagttttga tttgaaaaaa
    11341 atacctgatg aattaccgaa agcaatttat aattgatgaa gaatacaagg ttttcgcaat
    11401 caagaatacg ggcaaagaga cgtttaagaa gtagcgttat ttcatgtcgc ttgatcgtca
    11461 gatagataac aaagctccca actatcttaa acaggcatta ggttgcccct gtcacgcgcc
    11521 ttatgctgac aacgctggga ttcaactggc tttaggacag gtttttgaaa gagacaaaga
    11581 tcatttgttc aattactata gagatatgct aattgccatt tatgcaggat tagcggtcga
    11641 agagatatta ttgaacggat tgtcgaaagc tttagactaa atattgaata ctatttaaac
    11701 taattttgcc aaaatagtat tcgttttttt tatttatact atcatgattt gttttatcaa
    11761 caatagtttc gtgccatatc gggatgcaac gcttcatatt tccgatgtcg gcctgcaaag
    11821 gggctttggt attttcgatt attttatttc agtaaatggc gccataccat tttttgatga
    11881 ttatctaaac cggttttatc tatcagccga aaagttaaat ctgaaggtgc ctttcgatag
    11941 aggcgatttg aaggagaaga tctatcattt attaaatgaa aataagttgg caattacagg
    12001 gatcaaatta ctgctcacag gtggttttgc ggatgatctt tatacgccta ccgatcctaa
    12061 tttcttgatt attaatatac ctgtgcctaa ttattccgat acttattcgg atgggattaa
    12121 gcttctgctg cttgattatg tgcgatactt gccggaggta aaaacaacgt tttatttacc
    12181 tgcattaagc cttttgcctg aactgaagcg catcggagct ttagaaacgc tttatcatca
    12241 taaaggtata gtttacgaag caactcgtgc aaatattttc attattaaaa acggtgtttt
    12301 aattacacct tccgaaggga ttttgaaagg cattacacgc aagcatgttc ttaaagttgc
    12361 tcgattgcaa atgaaggtgg aagaacgaat agtaaaactt gacgaaattt ttgattcgga
    12421 tgagattttc attacgggca cttcaaaaca catactccca gttgtaaata ttgatggaaa
    12481 aatcatagct aatggacgtc caggcacaat aaccaataaa ttagctcagg attttaaagc
    12541 ttattatcag gatcagattg ataaaaaata gcctctttat ctatatctaa aaaaaattaa
    12601 tgacaaagaa atattctatt gtactccttc aagttaagca tcagaaaatt gttatgacaa
    12661 taaaatacta ttaccaacac gactattatt ccaatacctc taaagagatc tctgaccaat
    12721 tgtttatgtg aaaaattttc ataataactc atctgaaata actgattttt cgtttacgaa
    12781 aatatgtatc caaaaaccta cttacgtatt taattcacaa tttttaagag tgtttaagta
    12841 ttttttagaa aaccaatcaa tctctcttct tttatattgc attatatagc gcggatgttc
    12901 cagtggaata atttttttga aaagtttgag agatgaattg agctgggtca aaaatttaaa
    12961 atttttatcg ccacctacac atattgcttt gtgcggatta gctccgaaat aaaattgctt
    13021 gcgagtagat tcaacgataa atgggtaaac agcctctttt aatatttgta agtcatagta
    13081 attcaagttt ttaccatctt ttgtaaagcc aagcggcgaa agcgatgtta tataaaattt
    13141 cttgtaaaac tgttccaccc caccatattc cgtaatcact ttgtaaataa attgcgatga
    13201 taactcatgc ctttgaggga ggttattttg aatcttacac tcctctgcaa ggtttatcgg
    13261 atcggtgaaa gcaatgccgg tttgccctgc gccaaatctg cccggattta tcccaaaaat
    13321 aaatgtccta ctatgatggt catcataaaa ttttttgaag aaggtggctg tcagattaaa
    13381 tatttcaggt tttttatagg gattcatcac ctcgatacct tgaatattga tgttcagttc
    13441 aacctgttga aagaagtcaa tagcctgttt gccaaatgtt ttcataaaat gtgaaaaatt
    13501 atttttatgc tttcaaacgt caaaaatcgg ctatttatgc tacttttgtg gccggatcgc
    13561 ttttttattt attaaaaatt aattttcatg aaaaaaatct ctatgctact gatgaccatt
    13621 ttggttatct tctttgtttc atgtaagcaa caatcaaaac atgcaaaaga tatgaacccg
    13681 tttcttatgc agtacaatac tccttttaac gtgcctccat tcgataaaat taacaatgag
    13741 cattatttgc cggcaataaa ggagggaatc gaacaacaaa aaagggagat agaggatatc
    13801 attaataatg aaaagccgtc tgattttaaa aacactatcg aagctctcga ttattccggt
    13861 tatttgctaa ctgaagtgct tagtgttttt ggaaatatgc aatcggctca tactaatgat
    13921 gatttacaaa aaattgcgca agaggcttca ccgttgttat cacaacatag tgacgatatc
    13981 gtgttgaatg aggagctgtt tgctcgaata aagagtgttt atgataatca agaggagttg
    14041 gggctaaaca cagaagagct gaaattgctg caaaagactt ataaaagctt tgttcgtaac
    14101 ggcgcactgc ttaatgaaga agaaaaagca gagcttcggg aaatcaattc ggagttgtcg
    14161 ctgctctctt tgaagtttgg agaaaacctc ttagcagaaa ataacaactt ccagttgatc
    14221 attgatgacg aggccgatct tgacgggttg ccgcaaagtg ttcgtgatgc tgcatcagag
    14281 gctgcccaag aagccggaat gagcgaaagt tggcttatta cgcttcacaa cccaagcaga
    14341 gtgccttttt tgcaatatgc taataatcgt acactcaggg aaaaggtgat gaaagcctat
    14401 attcaacgtg gaaataataa taatgtgaat gataataaag ctgttgttga aagaataact
    14461 tcgctaagag taaagcgggc taatcttttg ggctataaaa ctcatgcaga ttttgtgctc
    14521 gaagaaacca tggcgaagac gccagaggct gtatattcat ttctccacga cttatgggag
    14581 cctgccctca acaatgctaa aaaggaagca tcagaattac aaaaaatgat ttatgattcg
    14641 ggcggagatt ttaaattaga atcgtgggat tggcagtatt atgctgagaa acttcgtcag
    14701 cagaaatatg attttgatga agaggcatta cgcccatatt ttgagttgaa gtctgtgctc
    14761 aatggcttat ttgatgttgt aaacaagttg tttgggatta aatttgtccc actgcaggat
    14821 gtgccacttt accatcctga tgctgtcgtc tttgaagtta ccgaggccga cggcgctcat
    14881 ataggtattt tatatatgga tttttatccg cgtgcttcaa aaactggcgg agcatggatg
    14941 acaagcttcc gcgaacaatt taagaaaaat ggagaaaata tccctccggt aatttctata
    15001 gtaacgaact ttacaagacc taccggtgat caaccggcat tgttgagttt tgatgaagtt
    15061 gaaaccctgt ttcatgagtt tggacatgcg ctacatggtt tgctctcgga tgtaacctat
    15121 cctaagttgt cgggaactaa cgtaccccgt gactttgtcg aactgccttc acagataatg
    15181 gagaactggg ctgaacatcc tgatgtgctg caaagctttg ctaaacacta taaaaccggt
    15241 gaaactattc ctgtagaact gattcaaaaa atcaaaaacg catcgaagtt taatcaaggc
    15301 tttgctacta ccgaatatat tgcagccagt cttcttgata tggattggca tacacttacc
    15361 gatacgatcc ctcaaaaaac caatttattt gaagaagaat ccatgagccg tgccggcctg
    15421 attcatgaaa tagatccacg ctatcgtact acctattttg cacatgcctt ttcatgggga
    15481 tattcagcag gatattatag ctatttatgg gctgaagttt tagataatga tgcttttgag
    15541 gtatttcttg aaaatggact tttcgatcct gcaacgtcaa aatcattacg cgaaaatatt
    15601 ttatcaaaag gcggtacgga ggatccaatg gagttattta tcagattccg tggtcaagag
    15661 ccttccaaaa cccctatgct tagaaataga gggttacttt aaaagttacc tataaaggac
    15721 tcttggaaaa gaatctgtta acttacaaga aagccagaaa cacattttat atggatattc
    15781 acgagtttct cgatcaggat cagcaaaagg atcttcttag gtttcttacg gcaggatcag
    15841 tggatgatgg taaaagcacc ttgataggca ggctgctttt tgatagtaaa aagctttatg
    15901 aggatcattt gcaggcgctt gaacgcgata gcaagcgaat aggtcatgcc ggcgaaaata
    15961 ttgattatgc gttgctgctt gacggtctta aagctgaacg cgaacaggga ataaccattg
    16021 atgtagcata tcgttatttt tcgaccaata aaaggaagtt tatcattgct gataccccgg
    16081 gacatgaaca gtacacccgt aatatggtaa ctggagcctc aactgcaaac cttgccatta
    16141 ttctgattga tgcccgtaaa ggagtaataa ctcaaacaaa acgccatacc ttcatcgtgt
    16201 cgttgttagg tattaggcat ttagtacttg ctgtaaataa aatggatttg gtaaactaca
    16261 gtcaggaggt gttcgaaaag atatgtagcg attataaacg ttttataacc ggcctcgaca
    16321 taccggatgt aaattttatc ccactttcag cgcttaatgg cgataatgtt gtggaactca
    16381 gtttgagaat gccttggtat catggggaga gcttgttgga atttctggaa aatgtgcatg
    16441 tcggtagcga tcataatttt agcgaactac gctacccggt acagttcgtt cttcgtccct
    16501 cattacattt tagagggttc tcagcacgaa tagcatcagg agtaattcgt aaaggcgata
    16561 ctatacgggt attaccttcc ggcaaagaga gcgtcgtaac ctcaataatc actgcaaacg
    16621 gtgaaagcga atttgccttc ccgccacagt cggtaacaat taccttgaaa gatgagatag
    16681 atatatcgcg cggagatatg attgtacatc cagataattt acctatggta aatcgccata
    16741 tggaagcaat gctcgtttgg atggacgaaa aacctatgaa gcctgaaact caatattata
    16801 tcaaactaaa taacaatact acaaaagtaa aaattgaaaa tatcctttac aagattgatg
    16861 ttaacacttt agaaaaatca aatattgagt tttttacatt gaatgatata ggtcgggtta
    16921 tcattaccac caacaaacct ttgttttttg accggtataa acatagtcat agtacaggct
    16981 catttgtgct aattgaccct atgaccaaca ataccagtgc agtagggatg ataattgacg
    17041 aggttaaggg gaaaggtagt ttttcgagaa tcactgatat tgatcgtaat aagattgagc
    17101 gtggcgagtg tttggttccg cttgatcaac gcgagcgctt ttatgatcaa aaaggtgtaa
    17161 ccctttggat aacaggactc catgggtcag ggaaaaatac attggcctat ttacttgaga
    17221 aacggctatt tgaggagggc gccaccacaa tattgcttga tggaagtact atcagatcag
    17281 gtctctcacg tgagcttgat cagttggaag cccatcgcgc cgaacatctt agaaaagtag
    17341 cacaagtggc caggatactg aatgatcaag gagttatagt tatctgctca tttatctcac
    17401 ccgatgaaga gatcaggaaa caagttgcag agataattgg gaaagagcgg tttctactta
    17461 cttatatgaa tgccgatctg gagcatagcc gtaattttag gcccgagctg tacgataaag
    17521 ctgatgccgg cttggtttat gatgtccctg gatatgatgc taaatatgaa atacctccaa
    17581 atcctgattt taactttaaa cctaatgaaa tggagacaaa ctgtgacact attatggatt
    17641 atttcaggga gtctggtgtt tttccgctaa agcgataaca aatttatctt atttttattt
    17701 aaactatcaa atttaattgc cggtttgtac tttattaatt acgttgtttt ttttaatttt
    17761 ttaataaata atttttcagt agcctgattt aaaattatgc aactacttag cttatcacag
    17821 cagtggggtt taccatttca aaatccggta ctcatctttt cattgatcct gtttattatt
    17881 ctttttgccc ctataattct taacaggttc aagattcctc attttattgg ttttacatca
    17941 taccacaata atatgttcaa aatccctgat tatttgaata aatattttac tgaaaacaac
    18001 tacttccttg tctatccaat acaatcaggt gttggggatg atgtatcgtt aaccctaagt
    18061 aacccctcag taatggagcc tttccgcgag atagaggagc ttagaaaaat tatcaccaaa
    18121 ttatttaaaa ataaatgatt agcttttaat tttaaaaatg ctctaacggt tgatgaagaa
    18181 atttatctcc ataatgcttt taatgttgtt gatagctcct cctgtcgtta cctttagctg
    18241 gctacatatg aaaaaacgta tgttgagaaa ggagattaaa tggaagataa taaatggcat
    18301 tgataaagag gagcttgttc tcttaacgtt ttcaaatgat caggcagaag catgtttgaa
    18361 atggcatcat tccgctgagt ttgaatttat gggagagatg tacgatgttg tggaaacgat
    18421 tttcttcgac gattccgtat cttattactg ctggtgggat catgaagaga ctttattgaa
    18481 taagaagtta aaagcaatgc ttgccctctc ggcaggaaag agtaaacaag aacgccaaca
    18541 aattaactat ataattcaat tttttcaatc tatttttttt attgaaaatc aatttggatc
    18601 aaactcgatg atttttaata atacacgatt ggatatcact tatttttttg tagtaaagac
    18661 ggcgggtatt attcccccag taccgccacc tcgattaaat agccgtttta tatgattgta
    18721 tagcaaaatt ttcttacctc attcattttg tttgttgtgg aaagatatgc ttaaaatgtt
    18781 attagtggga ttaattagta cgattatgtg tttggtgttg ttaaaaatct ccttacattt
    18841 taacgttaat tcatttttaa ataaaatatt tttcgggaaa gagaatccct tgctttcaat
    18901 cgaggttacc tatcattctt tactaaaaaa taattaaata ataatgaaaa agatactttt
    18961 ttttgtggtt attataataa taacacaagc aaatgttttt ggtcaaaagc taacaattaa
    19021 agacaaagat agcgggaggc cgctcaatct tgtcagtctt tcgagtgcaa atcctttagc
    19081 ctccgcaact acaaatactc gtggtcaggt ggatatttcc gatttcaaag gttctgatgc
    19141 tattgagttt cgattgcttg gatatcaggg gattacgtta agttataatg cattgaaaga
    19201 gttggatttt acaattgctt tatctcagtc acttttttcg ttggatgaga ttgtggtttc
    19261 tgctacacga tgggaacaaa acaaacgcga ggttcctaat aagatcactt ccataaatgt
    19321 aaaacagatt gagctgcaaa atcctcaaac agcagccgat atgttgggaa atacgggtga
    19381 ggtttttatt cagaaaagcc agctcggcgg tggaagtcct atgattcgtg gctttgcaac
    19441 caacaggtta ctttattcta ttgatggtgt gaggatgaat acagctatct tcagagccgg
    19501 aaatattcaa aatgttattt cagttgatcc ttttagtatc gaaacctctg aggtgttttt
    19561 cgggccagga tcaaatatct atggtagcga cgctattggc ggggttatga ttttcagaac
    19621 gttgacacct cagctttccg aaaataaaga cccattggta aaaggtaagg ctttagcacg
    19681 ttactcttct gcaaacaatg aaattacagg gcattttgat gtgggcatcg gttggcaaaa
    19741 gtgggctttt acaagtagcg ttaccaattt taactttggt aacctgatgc agggaagccg
    19801 tggtccggat gattatttga aacgatacta cgtgaaaaga gtagatagcg ttgacagggt
    19861 gtttgttaac gaaaacccac gattacagac gccatcaggc tatacccaaa ccaatataat
    19921 gcaaaaggtg aggtttaagc cagacgaaaa atgggatttc aattatggat ttcattattc
    19981 cgaaacttcc gaatatgcca gatatgatag aatggctgaa ttatcaaaag atgggctgcc
    20041 aaattccgca gtatggaact atggccctca aaagtggatg atgaatcatt tagagataac
    20101 ccattccggt ccaaatccgg tatatgacca attagttatt cgtgtagctt atcaaaaatt
    20161 cgaagagagc aggattgatc gtaaatttaa caacaaccgt ttacgtactc aattggaaga
    20221 agtggaggct ctgtcgttta atgctgactt tttaaaaaga tcaggaaaaa ataatttttt
    20281 ctatggcatt gaagccgttg taaatgatgt tgtttctact ggctcggcag ttaatatcaa
    20341 aactggaaaa ccagtggatg tccctgatcg ttatcccgac tcgaaatgga aaacttatgc
    20401 agcgtatatc aactatcaat atcgcttgtc gcagcaattg cttttacagg ttggcgggcg
    20461 ttattcgcag tattttatta actccgattt tgataagtta ttagcttttt atccttttga
    20521 ctataaagag gttgaggtaa ataaaggctc gcctaccgga agtgttggcg cagtatatac
    20581 gccaacggat aaatggacac ttagcacatc gctctcaaca ggatttcgcg ctcctaatgt
    20641 tgatgatata ggtaaaatgt atgattttaa agctggagac gtgatcgttc ccaatcctga
    20701 tctgaaggct gaatatgctt acaatggtga aatcaacatt acaaaagtat ttggaagcag
    20761 tgtgaagttt gacattacag gattttatac ctatttggac aatgccatgg tacgtaggga
    20821 atatactgta aatggtcaag acagcattat ctacaacgga gagatgagca aagtgtttgc
    20881 tttgcaaaat gctgctgatg cttttgttta tggtattaat gccggattgg agatcaagct
    20941 gcctgaagga ttcggtattt caggccgata caactatcag tttggcgaag aggaaatgga
    21001 agatggaact accagtcgtt cacgtcattc agcgccttcg tttggctatg caaaacttac
    21061 atacaacgcc aatcgccttg atatgcagtt tagcgctgtt tttaacggaa agattgatca
    21121 taaagatatg aatatagaag aacagcagaa aacatccatt tacgctgctg acaaagatgg
    21181 caatccttat gttccaagct ggacgatcct taactttaaa gcaatgtata gattaaccga
    21241 tatgtggaca ataagtgcag ggctggaaaa tattaccgat cgtcggtata ggacatacag
    21301 ttcaggaatt agcgctccag gacgaaatat ggtactttcg cttattgcta atttttagta
    21361 aaatcgtatt aatcaattaa attgctttct aataccaatc agatagctgt tatgtgttgg
    21421 taaaagtttg ataaatggcc atctgattgg tatttcttct ttatttcttt accctaagcg
    21481 tgctaaagat caacttaata tctgtcattg atggaaacga attcttgagt ttgatttttg
    21541 attcacgact gaaaaaccat actactgtta acgatgtaat caggtgtgaa atagtagttg
    21601 cccaaccggc tccggttatt ccataaacag gtataagtag tctgaaaaat agaaccgcaa
    21661 acagaagccc cgcaaaggat gagatggtat taacataata tttaccaata cctgagaagt
    21721 aatgtccaat gattagcgcc gtattaaaaa acaaaactcc tggcgctaat gcccgaataa
    21781 cagaacttac ttcgccgaag ccttctccat aaaggaaaat gaaaaaccgt gaaggtagca
    21841 tcaccataaa caagagtccg ataaaactga ttattaaact cgcccagtta agttgcaacg
    21901 tcagcttttg ggaatattcc aaatcggcgc tgttagcaat acgtgcatat tgtaccaagc
    21961 agatgctcct acttatcagc cagatagatt ctacaagtga tgtcccattg gaataaatcc
    22021 caacctcggc gcttgaatag gtttgttcta accagtaata actcattcta aagctcagca
    22081 gttggaaaat atgcgccagt tgattaagaa ggccgtacct gaacaagttg cttataatag
    22141 ttttgtaact tgatatgtca gcaagttcaa atttatcggt aaagcgtttt agataaatca
    22201 ggctgacgat aaaaccagcg gaaaaggata tataaagcga gataatatag gattcgatag
    22261 tgttttttcc gagataaaaa aagaaaacaa ttagcaatag agtgatgatc aatggttgga
    22321 taaaattgac aagattggat attgcgattt tttctttgcc aataagtact gatgagttaa
    22381 ttgtagtaaa ggcgctaata gcagataaaa tacaaacatg tattatcatc ccttccgata
    22441 ccagagtaga aagctgaaga aaaccataaa ataaaatact tgtcaggacc gtccacagat
    22501 aagccggtaa aatgataacg gaaaccggaa atctaggtac aagatataca attgcgctgc
    22561 cccccatcag attgacaaat accagaatat acgcaatggt agcaataatt atcccttgtt
    22621 cacctttgcc ttcaggacct aaaaattggg aaatcagaat ggttatcagc agatttagca
    22681 ttgctgatag caaacgcgta ccaaacgtat tgaggatttt attcagcacg gtttatcttt
    22741 tttatattaa cagacctttc tttttaatga tggttaggga caatatgggg atacaacgaa
    22801 ttgatgtgga tttgcttagc ttgcgattag ctcaatgtaa acgttatata ttgaattgcc
    22861 aataatttcg ttgctgaaat tattcagtgc atattctcta attttagcgc tttgatattc
    22921 tttcggattg tctaaaaact cttcaattgc ctctaaaaac tgatcttcat ttcctggctc
    22981 ggtaagcctg ccaagtgtgt cgttaaggtg ttcatgaata ccacctaccc ttgtagatat
    23041 tacgggtacg ccgcaggagt agctttcgag aattactacc ggtaggtttt cgtagttact
    23101 gaacattatc atcaaatcgg cctgattaag caattccaca agagcttcgc cctctttaag
    23161 tccagtgaat tcaacaaatt tgccagtaag gccaagctca tccgacattg atttcatagc
    23221 aataaaatct tcaccgtcgc caattagttt tagaaagaaa tcatctcttt tttcacttag
    23281 ctttttcatt atccgcaaaa ttcctgaaat gtttttttgt ttatcagtga agcacgaaag
    23341 atggactaat gattttttat tcggatcgta aacttttggc gccggtttaa acttttcaac
    23401 atccactaca ttaggtatta cgatgtaatt tttattttta agccctttgg cgatcattgc
    23461 atctctaagg tttgcagtaa caggcattac agcgccggca tgtttcacaa caattcttgt
    23521 gagccacttt cttaaacctc cataatagct gtcggtattg ggcagatatc gtgtccagtg
    23581 ttcggtaatt gcatagggaa cgccggtaaa tatcttgtaa atcaatgcaa taataccaca
    23641 gcgagttaaa acgtgaacat gtaataaatc gggcttcgaa tactcgtttt taatgatttt
    23701 aaagcctgat atgtgacttc gaaaaaaacg gtagaggttg ataagatttg caagaaacct
    23761 tggtttaatc attgattttt taaaatacac ccttaccgta aataatccct cttttgaagt
    23821 tacaatttca taacttcttt tggttaattt ttcatcatca tgaacataaa gtaccgatat
    23881 atcggcatgg ggcaatactg aaagcccgtg acgttcaata aaaagcccca tcatgggatc
    23941 gtaacgatga ggataccaac ggggcagata taataccgaa agtcttttgt ttgtaaagtc
    24001 ttttttcatt cagtttgaac gctatttgat tttttttatt taatcagttg gttgatataa
    24061 tttattgcat tttgaagtct tgtttcgcca atgccattga cgatactgta attgaaccca
    24121 tgtctgtcga gctcttcgat atattttttc atcaaaaact ctctacgatc agggtgttcg
    24181 cgtagggggt caaactccca tggcagatca atatccatta aaagataaag attgtatttc
    24241 tgccgtatta gttgcttttc gatccacgaa tggcattttc cgtatttaac atcgcaccag
    24301 atttttgtta ctatcaattc ggtgtcggaa aaaaggatgc gattggcgag agtagaaatt
    24361 gctttttccg aacttatttg ccctttcgct atttcaagaa tgtcatcata attatagggt
    24421 tcatttttat tgagaaggta agcccgtgca aattccggta cccaaacgct gttgtagtgg
    24481 gaggctaatt gttttgaaat taacgatttg cctgttgatt caggcccggt tatggctatt
    24541 ttgatgagtt tgctcaactt cggattgttt tagtattctt ttccattcga tataccccag
    24601 tacggctaag ataaggaaaa caaaatattg gaaacttgtc agtacaagct ctttatgaaa
    24661 gtacaaaggt acggatacta aatcgccaat aatccaatat atccagtttt caactttttt
    24721 tagcgccatc aaccccattc cagtaatgaa tatggctgtg gtgaacgagt caatgtaagg
    24781 tatggtgcta tcagtataat gcctaaggat gtattgtaga attaaaaagg agatgatggt
    24841 taacagtacc caaataaaat ggtgtaacag cgaacatcgg cttatcgcca attctttgtt
    24901 ggaggaatct ttacgtgtcc atttgtacca tccgaaaaca cttacaagga agtagaaaaa
    24961 gttaatcccc atgtcggcat aaatcccagc atgataacag atgtaaacat agatcaaaac
    25021 agaaatgata cctgtgggat agaccagaat attggctttt tttgcatacc atacgcttaa
    25081 caggccaaag atcacagcta ctccttcaag ccaggtactt tgtaagagat tttggtaaaa
    25141 tagatcaaaa aaagtagtgg ttaacatcag gatggattaa gatcagcatt gacaattttc
    25201 attacaaaaa tggagtgcgg caattcaaat gaccggcaaa tttcgtcagt aattgctttt
    25261 attatcatgt agtattcgct aaaaatctgt gtgatcaact tgttgctttt tattgtcagg
    25321 ttttcatgtt ttatttaatc tgctgataaa atctttgacg gcagagatat agtcttcatt
    25381 tggtgggtat tagtttatct tgatggatat attcatattt tctaaggagt gatgtaattt
    25441 ttaatttcag caaaagtaaa gaaaagtgca ggaataggtg tcttttttct tcaacctgat
    25501 ttcagacaaa aaaatagttt tacttttgca ctcctaatgt tggtccggta gctcagttgg
    25561 atagagcagc agccttctaa gctgcgggtc gtgggttcga atcccgcccg gatcacaagg
    25621 ccctgtaaat ttttaagttt cagggctttt tttatttggg ttactatgtt taaaaataca
    25681 gacgagctgc tgtaataaat ttatggcccc tgtttcatta ttcgatcgta ctgtgcagcc
    25741 tttgacgggt caatctcctt cagcatattt actgcttttg ttttatccat gccggctgct
    25801 tgcgagtaga tgttaatgaa ttcgtctctt tttgaatcca taatcagttg gaagatgtaa
    25861 aggttgggtt tctcgcgata tgctcgctga agcgtttcaa gcgctaatgt tactccctgt
    25921 cttccctttt ccatgttgtc ctgcataaga tcaagtccaa gacgatgaaa ctgataattg
    25981 aacttacgaa catttgcata agaagagttg gtgaggtttt cggctatcca gtagcggttc
    26041 cggatactct caaacgactt ccagcccggc tcttttgcgc tttgtgcact attaactatc
    26101 tgttgtgcct tttcaaaata gggagtgcct cccatcaact gaaaagagtc gaaatccaat
    26161 ccgagaaaga tatatatgta gaaggctatg gttgacgtga ggttggatgt aaacctatta
    26221 tcttcaaatt cgatagcttg ccctggctgc cattgaaatt gaaagttggg atcctgatag
    26281 ttgaacatgg ttgtgctgta ggacgtgcca taaaccgggc gttttaaaac tagattcatc
    26341 tttcctttga actcatcgga gcctatctct tcggtgatgg tgatagcaat tgttgcttcg
    26401 atccgctcgt tgcgagttag agtatagttt gtccagcgcc gctcgttaac gaattcatat
    26461 aacccttgtc gtaattcatt aaacttctct ctattggttt gttgaacttg cggagctgaa
    26521 atctgaacag aacaaaagaa ttcttgtgct tgaactttgt taaaaatgaa agctaataga
    26581 ataatgctac aaaaagagag aattatgttt gggtatttca tgctttagaa tgcttgtcct
    26641 tttttaatca ttgttgttac tacatctaag atgtcggaag cgacttcttt tttagtcttc
    26701 aatccatatt cccatttttt gccactgtta tttatcagtg ttactttatt tgtgggatag
    26761 ccaaaacctg ctccttcatc ctttagagaa tttaaaacga taaggtcaag atttttagta
    26821 ttgagttttg tctttgcatt ttctatctca ttatcggttt caagggcaaa gccaatcaga
    26881 atttgatttt tttgttttag tttaccgagt tccaacaata tatctttcgt tggttttagc
    26941 tctatcacag gtgtgccgga ttctttcttt atttttttgt ctgacggatg tgatggggta
    27001 aaatctgcta ctgctgccga cataaatact atatcaacat gggcaaattc tttgattgtt
    27061 tgctcataca tttcatttgc tgatagaaca tcaatgcatc tgatcgcctt attatctgat
    27121 tttaggtgtg ttgggccgga aatcagggta actgtcgctc ctctattagc gcactcttca
    27181 gcaattgaaa agcccattag tccggatgag tgatttccga tgaagcgcac tggatcaata
    27241 ggttcgtagg ttggcccggc tgtaacaagt attcgcttac cggtcaactc attgttgtgc
    27301 aaaaaaaaag cgttgataat ttttaagata ttttcgggtt cttcaagccg acctgcaccg
    27361 gttagcccac tggccagttc gcctacttgc ggttcgatga gcctgtgccc aaaagctttt
    27421 aattgttcaa tatttttttt ggttgatggg tgattgtaca tgtcgaggtc catggccggt
    27481 gcaaaaaaga ccggacattt ggctgataaa tatgtggcca ttagcaggtt gtcggccaaa
    27541 ccgtttgcca ttttgctaag cgttgcggct gtggtaggcg caatgagcat caggtcagcc
    27601 cagttgccta attctacatg gttggtccaa gagccatctg cggagttgaa agggtaggtg
    27661 agggccggat gttttgatag ggttgaaatg gttaaagggg taacaaaatc ccgagcattt
    27721 tcggtaagca atacctgcac ttcggcaccc tcctttacaa gaagccggat taggaatggt
    27781 attttgtagg ccgcaatact acctgtaatg ccgattagta tttttttgcc actaagcatt
    27841 aaaggttata atctttggtt gatttctttt ccagctcttt cgatggatta cgatagtata
    27901 tctcatcgtt tagaaattca tgaatagcaa tgaggcttgg cttaggaagc ctttcgtaaa
    27961 atttagagat ctcaatctgt tctctgtttt caaaaatttc ttcaagattg gtagtagtta
    28021 aggcaaattc ctcggttctt ttctttagct catcacgcat ctcgatggat atctgattgg
    28081 cgcgtttgga aagaattaca atagtttcgt aaatattgtt agtcccttta ctgaaatccc
    28141 taaccctacg agtaactgct gtaggttcga ttttaatttt tttataatcc attaatgaaa
    28201 ttttaattaa aagttaaatg ttatcagagc cttttttagg ttgatcaata gaacgcttgt
    28261 aatatttatt tagttcattt acataacgac tttcgggaaa taacgtaata aaatcataat
    28321 aatcattttg agcgctttcc aacctttctt gtttcttttc ttgaatacta ttgttagcaa
    28381 agttatatgc tgatactaca attttataca tgatttcttc tttataagcg atttcaggat
    28441 aatctttcag caaatttcgg aatgagacaa ttgctgcctg ataatctcct atcttcatat
    28501 agagattggc aatattataa tcctttttct gaagtttcaa cctaagctgg tcaattaatt
    28561 ttgtagcttc tttagctttt tcaccgtaag gatatcggtt gataaatgat tgaaactcgt
    28621 tgattgcatc ctttgttacc gtttgatcaa gggtgtgtct gggactttca agatatttgc
    28681 aataggcagc catataagcg gcttcttccg cttttgagct taaagggaaa gtagaagaaa
    28741 aacgattgaa atgataactt gccattacgt agtcgcgctg gttgaagtaa gcaaaaggat
    28801 aacgaaatag aagatcttcc gatttagatg ttccgcgata tatcgggatc aactgatcaa
    28861 aaagctgcag agctctgtaa aaatctcctt tatcataata ttgcatggcg atttcgtatt
    28921 tcttttcatt gtcggtgctt ttaagcaatt tttgatgttt actgcacgac aacaaaatgc
    28981 tggaaattaa tattatggag atgaaaatcc tttttttaaa catggctgca aaggtaatat
    29041 tatcattgga tttaaaacga atatggtgat gaaatatttg caaattactt gtttcttatt
    29101 gctttaaagt ttgttttttt ttaaagctaa aggttgaatt caggtcttgc agaaagaata
    29161 agcaatgtct tacatcctta tccatacgta ttcgaatggt tttaatgtaa atgtttgcag
    29221 gttttggtca aaatctaacg ggttgtcaaa aagctctttc catgttgagc ttggctgtag
    29281 tgcaagccgc ccttcttgtt ttgatgaaga tagattttgg ataataagaa atcgttcttc
    29341 attagttttt cggatataga ctaataacga attattcttt gtgccgtctg gctttaatgc
    29401 ttctaaccat tcaatttgac cacgccctaa aactttaata tcttttctga tattcaccat
    29461 ttttgaaatc ttatgaaaca cttgtgaagc aaatgaatta ggattttcta agttgttttc
    29521 caatgtttcc caatcaattt ttccgcgcac taagaaacgt gtatcgtctt ttttagtttt
    29581 tctgatttgt tcttcgtaat aggcttggtc gttaagtttg ccatattcgt cgccataata
    29641 gataattggt gttccgggca gagtaagcat tatcgaatag gttaatgcaa ttttatcagg
    29701 gtttctcttc atcagctcag caaggcgagc agaaatgcct tctcctttcc tgaaatccca
    29761 ttgcgagtca tgacagtaat tttggtgaat gagggctctg tcttgttctg ttacatagac
    29821 taattcgagg cttagctcat cgtggcagcg caaaaaggta aaccattgtg cattatcagg
    29881 aatttcgggt gtaacttgtt tgcttagggt gtaaagtata ggttcgctgt tttctaaggc
    29941 taaggccttg taaatttgtg gcattaatgg aaaatgatat ccggcattgc actcatcccc
    30001 atcgccaaaa tattttacga cctcaatcgg tttttgacaa gcttcggcaa gtagtaacgt
    30061 atgaggctgg acaaaatcaa gcgttgctct gaaaaactta actatcgtat gtgtctgagg
    30121 aaggttttcg cagttggtac catcttcttt ccaaagataa ggaattgcgt ctgctctaaa
    30181 tccatcaacg cctttttcga gccagaacaa caggtttttg gccatctcaa tcaatacttg
    30241 aggattacga tagttcaggt cgggttgaaa cgaaaaaaac ctatgaaaga aatattcgtc
    30301 atcagcaggt tcccagttac ttgtttccat acctttaaag atgattcttg catctttgta
    30361 aagatctgta ttggcgctcc agatgtaaaa atcccgaaac ggattagact ttcctttttt
    30421 tgcttcgaca aaccaaggat gttcctccga tgtatggttc attgcaatgt cgaaaataat
    30481 tcttatattt cgttgatgag catcctcaat gaactcttta aaaacagtca ttcgttcctc
    30541 ttccgaagct tcaggtgata atcccaacaa ctcttcacga atttttttat agttccgtat
    30601 atcaaatcct gcatcgcgca ttggcgaatc aagaacagga agtagccaga ggcagtttac
    30661 cccaagtttt tggatataat caaggcgttg gcgtaaccct acaaaatttt cattgaacaa
    30721 atcaacatat agcgaataga caattgcatc cttataccat tccgaaggtt gaggactgat
    30781 gtttaagttg tttttaaaat ttttaatata gtggataaaa ttggaaagtt catgctcata
    30841 ggcattgcca taaagctgtt tccaaagcga ttttaatttt ttgataatct ccattttgat
    30901 gatagtttat tactgattaa aatataatct gcaagttggt aatagattcc gacaattgaa
    30961 attttatttg aagtaattct ttctcgctat cccagaacga aatctgtgtt ttagtagttt
    31021 gataggcttg tttggaaagc gcatttttaa cgggaactct tccggtttga attttgccgg
    31081 gcttggaatt gtatccgtga attaataatt caatattgcg tgtagctggt ttgccgttgt
    31141 agttaaatgc ctctttacga ttaaaggtaa aaagtacaaa gctgtcgaag tgcattgatt
    31201 tgatctccaa aagctcgtat aatccttttt cataagccga ttttgtctct ccatcatcat
    31261 cgtaaataat gaattccgaa tttatgattt cgggatcata ataatggtgg atgatcaggt
    31321 tttcagatgt gtatgctgta gtatttttaa tgggattgat cattggaata aaacctccgc
    31381 cacgtgcaaa taccggtatg ttctccaggg ttgttgattt ctcgatccat ttgccgccgt
    31441 tcattacctg atcggtgaaa aagtcatacc attttccttt agggaggtac acctttcgca
    31501 tttttgcatc tttttcaaga attggcgcta tcaacagatt tggtccccag aggaatgtgt
    31561 cgtcaatatt gctgatttca tcattatcag gttcttcaaa aaagagaggt tttgtcagtg
    31621 gagttccttt aatgctgtta agccatgcga tcgaatagtt gtaaggtaac atcctgtacc
    31681 gcaggtcaat atattccttt ataatttttt gtgcattatc gctatatgca attggttcta
    31741 cgggtgcgtt ggtgtcgccg tgtgggcgga aaacagggtt aaaagcgccg tattgtagcc
    31801 atctcaggta aagctcttca tcaggttcgc ccatagcaaa tcctcctaag tcggagtgca
    31861 tataagaaaa cccgctaagt gtcatgccca gaacagcaag gggctgtact tgaaatccat
    31921 tccagtctct tgatacatct cccgaccatg gataaacgct gtatcgttgc gaaccggcaa
    31981 aaccagagcg gttgagattg aaaagcctga cctcaggata ctcaaggcta tagtagtccc
    32041 aaagcatttt atgccaataa tggctataaa ggttatgtac cacttcggcg cttcctataa
    32101 aatgttgcat tttggctgga tgtttttccg gctcacccag atcgccccac catccggcaa
    32161 tgcctatttc gatctgcctg cggtattgtt cccagaacca tcttcgggca tcgggattaa
    32221 aaacgtcaat caaccctccg gggccaaacc atagatcgtc aattacaaat gtattaccat
    32281 tattatcggt tcccatgtat ttgttgtcgg aagctaattg ccagtttttg gactcctgta
    32341 ggataaatgg ctcagtaata agtattgttt ttattcctct ttgtttaaaa cgttgaatca
    32401 tctgttcagg ttccggccag ttttttttat accagtcgag gtttcccatg taaaaatgat
    32461 cgtgaaatcc caatccaaac caataaagat caatgatgat ggcatctaac ggatatccgg
    32521 cttcaatcat ttgatcaaca atagcttcag tttcgtgttg agttttgtaa ccaaatttcg
    32581 actgtaaatt acccaatgcc caacgtggtg gcataggctg atagccagta aggctcccat
    32641 attcttttaa aatggagtca aaatcgttat cggcaataac gtaatatgac atttccccgc
    32701 cgattgagct gaattccatc acattatttt ggtgaacatc aaggtcgagc cagcctttgg
    32761 atgcattgtc aaacagcaat ccgtaaccct ttgaggatat aataaatggc acagagaagt
    32821 tcagattggg agagttcaaa ttataactgt aaacagcttg attatataac tcgtagcgat
    32881 ggcctctgcg gttgagaggc gtggtgcgaa atccggcgcc ataaatcttt tcatcaggtt
    32941 ttaacgaaaa acggaaacct tcaacttcat ttgatttaaa atagccatcc gactcggcca
    33001 atatttgatt ccctttttga aaaaagatca atttgaatgg cgatttttta attactacct
    33061 gtagtttatc gcgggtataa aaaagctcat tttcgtcttc gaataaagcg ccgcctgttt
    33121 tgtcggcaga tagaacaacg gagtgtgaag taacctcctg atctgacttt ttttgcgcaa
    33181 aactaacctg tacaatggat gaattaatga aagaaacact aatgctgcca agcgatgttt
    33241 tgattaccaa cttgttatcc gatagatggt gggagatata ttgattcttt ttttcctttt
    33301 gaaataaaga gcaattagtt aggtaattag ccacaaaaaa tgcacttgct aaaatcccta
    33361 agatgattag aggaattact atttttttgt tcacgcgtat aaaggtttgt ctgtttttga
    33421 tctccgtgca aaaatagagt attgcacaat atgtaatgat ttgaatttta tctgacgaga
    33481 agaggattat atcaatcgct aaatatggta cataatctcg tttaggtgga aatagcaaaa
    33541 ctggaactcg cctcataaaa ataaaattga tggttttata cagaagaagc gtgttgttgc
    33601 ttaaaacggt gttaatccaa ttattgaagt tctaatagtt gccaaatatt taatgaaaga
    33661 atgatataat tttgcggaga attggctaaa tttcttatcg cgaaagtttt acaaggagtt
    33721 caattattta tgtcaaaaca attttcagtt ttactatcaa cagcatttct tccacccatc
    33781 caatggtttg tctatttatt gaaagcagag aaggtgttga tcgaaaactt tgaaacttac
    33841 ccaaaacaga cttatcgcaa tcgttgcgag attgccactg ctaacggaaa gcaggtttta
    33901 acaatccctg ttataaaaat ttttggaaat catactaaaa caaatgagat tgagatcgct
    33961 aacgatgaaa aatggcaaat acgacattgg cgttctttag tggctgctta tgcaaattcc
    34021 ccttatttcc tttattatca ggacgaactg caaccatttt ttttggagga atatattaat
    34081 ttgatggagt ttaatctgtg cataataaaa tctctaatgg ctattattgg gatttacaaa
    34141 tctattgagc taaccgatac tttcgaatta aatccaaaaa atgtttttga cctaagaaat
    34201 gaaattactc ctaagaggac ttttgaccac tttcatttac caacatatta tcaggttttt
    34261 gaagaaaaaa acggcttttt ctcaggttta agcatcattg atcttctttt taatatgggg
    34321 ccagaaacgt tcgatgtgct taatagcgtg aatgttaatt gttgaaacct ctctgattct
    34381 tttcgatagc gattatcccg gatattctat tctgagatga tatatattca ttagcttacg
    34441 tttgagaatt tttcgtattt gggtaatatc gcggagggta atattggaat ttgaaaactg
    34501 acccgattct acctgtttgt caataatttt atctactaat gtactaatgg atttttcgtc
    34561 aacaactttt aggcttcttg atgctgcttc taccgagtcg gccatcatca ccactgaggt
    34621 ctctttggag aaaggaacat gccctggata gctaaaaagc cgttcgtcaa tcgtttcatc
    34681 agggttctct cttttttgca taatataaaa gtaatcaact ttgcgagtgc catggtgggt
    34741 gcgaataaaa tcaattatct gatcggggat attatttttt ttagcctctt caattccctt
    34801 tgttacgtgt tcgatgatga ttgacgcact ctcttcgtag gtaagctcgt catgaggatt
    34861 tactccggta agctggttct cgatgaagta ccttggatta tccatttttc caatgtcatg
    34921 atatagtgcg cccgtacgtg taagtaaagc attacctccg attatctgga tacattcttc
    34981 agcaagatta gccacttgaa gcgaatgttg gaacgtacct ggcgctttca tcgagagttt
    35041 acgaagaagc ttgctgttgg tatccgacaa ttctaataaa gtaacatcgg taactaatcc
    35101 aaaaagtttt tcgaaaagat aaataatagg ataagaaaga agtatgagca aagccgaacc
    35161 gccaaacatt gcataattta tgagtcgtat ctcatcaaaa ttaccttgtt gcaatagatt
    35221 aagaccggtg taaataacag aatacgatac aaagattatc agtgaggtta ctaaaaattg
    35281 cgatcggcgc gtaaggttga tgatgctgaa aatagtaatg atacctacaa tgagttgagt
    35341 aaagacaaat tgaaaactat tgggtacaag gaagcctatt agtataatgg tgattatgaa
    35401 aacgtatagc gcaacgcgtg tgtcgaaaaa cacactaatt attatgggta cgatacataa
    35461 tggaataatg taaatatatc cttctccgaa tttcatcgta agactggtta caaataccat
    35521 taggataatg gttaggagaa tcagtactat tttttttgta tcttcaaata tttcctttct
    35581 gaaaacatat aaaaagagaa tcaacacgag catggagaca cttacaagga taacctgccc
    35641 taataaaatc aacctgaagt tgctattatc gcttgctcta tcctggtact ctttttttaa
    35701 cgattcgagg atttgatatt tagcaagtgt aactacttct ccttttgaga ttatccgttc
    35761 gccctcttgt accattcccc gggtagtaga aatgttattt agtttatttt ctacctctgc
    35821 taacgtaacc cgttgattaa aaagcacatt ttgagtgatt gcctcttcta aagtttttat
    35881 caaaagttct ttatcagcaa attcagcctc ctcaactttt tttagcattt ctgaataggc
    35941 attatggatg gtataaaaat cggatagctt ggctctttct gcagtattgt caatcagtat
    36001 cataatatta tgatcgtctg atttgttatc aatctccgat actgattgaa gcacccctct
    36061 tgtatagatc agcgagagga tgtcgagacc tgtctgggat gttgaacgtt tcagattctc
    36121 tttcacattg atgctgccat atttcgaaac ccatgcttta tctaggtttt tgctgaactc
    36181 gctgcgtttg ttttcgtaaa ctgctaaatc gaattcaaag aagagttcat tattctggat
    36241 gatttcttgt ttttcttttt caagctcgtc ggccggtttt aatattccaa ggtcaaaagg
    36301 ggcgatgaga tcattatgaa gccagggccg atcgcgggta taatcgtatt tgaattttgc
    36361 ctcctttggg aaaaaggtaa ctaaaatcgc tatgctgatc agaaagagaa atgttttctg
    36421 aattgtagaa tagttttgtg tgatctttga ccaaaattga ttcataaggt tttgaataat
    36481 tcgctgctaa aataacaatt tctattttgc tgtttggcta attcattata ttcttttcgt
    36541 tgctgaacca ctttagaata gaagaatatc tgtaaaattt acatagaaat tgttgttttt
    36601 aacttttaag tgttctgttt tatcctttat atttgcaaaa aaagaacatt ttgaaaatag
    36661 ctgtaaatac aagattgttt attaaaggga ggcttgaagg gattggttgg ttttcttacg
    36721 aatgcctgaa acggatcacc actcagcatc ccgaacatca gttttatttt atcttcgatc
    36781 gtaagtttga cgaagagttt atttttgccg ataacatcga acctatcgta gcttttccgc
    36841 aagccaggca tccgttttta tattatttat ggttcgagca ttctattcca agagtgttga
    36901 aaagggtagg ggccgacctg tttttttctc ctgacgggta cctatccctt tgctctaaaa
    36961 cgccatcggt aaacgttttt catgatttga actttgagca ttatcccgaa gaccttcctt
    37021 actttgagcg taaattttac cgtcattatt ttccgaagta tgcccataag gctctccgta
    37081 ttgccacggt gtcagaatat tctaaaaatg atattgtgag cctgtatggt gttgataacg
    37141 ataaaataga tgttgtttac aatggcgcta acgaatcttt cggacctata tcagagcctg
    37201 agaagcaagt tgtaagagag cgtttcgcgc atggagctcc ttattttttg tttgtaggtt
    37261 cgcttcatcc acgtaaaaac cttgcaaggc tatttccggc ttttgatcag tttaagattt
    37321 cggataatga tagtgtaaaa ttggtgattg taggcgaaaa gaaatggtgg acagaagcta
    37381 tcgaaaaggc ctataaccaa atgaattaca aggacgacgt tatttttacc ggtcgtttgc
    37441 ctgttaaaga tttgcatcag gttattgctt cggcgcttgc tataacttat gtcagttatt
    37501 ttgaaggttt tggaatacct attctggaag ctttttactg tcatactccg gtcatcactt
    37561 cgcaggttac ctctatgccc gaagttgccg gcgatgctgc aatcttggtt gatcctatgt
    37621 caataagttc gatcgctgat ggaatggcaa gaatggcgaa tgatgaaaat ctccgtaagt
    37681 ctttagtcga aaaaggtatc gaaagatcgg cgatgttcag ttggcaaaaa tcgtcggaac
    37741 gtctttggaa tactatccaa aaggctttag atacaataaa ccaagaagct aaatagtgat
    37801 attgagtgtt gccaatcttt agtaaaagaa tattttatag attaaaaaaa gcttgtaatt
    37861 gtgatttttt gaattattaa aaaggaatgg aagaaaaaac aaaaaaagca gatcaacaaa
    37921 attccaataa gcttgaattt aatctagatt gcactccgac aatagataaa gtgagcgagc
    37981 ctaaggtttt gaattataat ctgaaaaaga gaactaataa gagagttgac gagagttggg
    38041 attttcgtaa tgcaaataca aaagagttca ctcattgctt tcactcatat cctgctatga
    38101 tgatacctca agttgcaagg cgtattatta atacgtatgg aagcaatgct aaaatgttgt
    38161 tcgacccata ttgtggaaca ggaacttccc ttgttgaggc ttgccttgcc ggaaaaaatg
    38221 caatcggtac tgatattaat ccacttgcac gacttatagc aactactaaa acaacaaaac
    38281 ttgatataca agttttagat ttattttttc acgacttcat ggattacttt tttgctctca
    38341 attttcaaga tgatggaata aaatcaatga tcatccccga ggtgaaaaac attgattttt
    38401 ggttttccaa atcagttcaa cgaaaacttg gaattttgct tggctacatc gaaaacatca
    38461 acgatatttc aattcgtaat tttttcaaag tggcctttag tgaaactgta agagaagtct
    38521 cctaccaaaa gccgggggaa tttaaattga tcagaagaaa agattttgat aaaagagttg
    38581 agcctgatgt tattggaata atgattagta agttgacaag aaatagacgt ggtttaattg
    38641 actttgtaaa aagttatgac aacaactcct ctatatctat ttatgacttt aatacggttg
    38701 agaatatacc aattaatatt ttaaaagatg aaagtgtgga tattgttgtt acatccccgc
    38761 cttacggcga tagtagaact actgttgctt atggacaata ctctcgttta tcgaatcagt
    38821 ggttaggttt tttgcatgct aatcaaattg ataataagct tatgggagga aagaaatcca
    38881 aacaacttta tgagttcaaa tgcgattttt tgaatggtgt tatttatgcc gttcaaaaac
    38941 aagatagtga gagggtgcgt gatgttgttt cgttttataa agattatgag aaatcaattg
    39001 gccttgtttc aagaactttg aaaaagggag gttttgtatg ctatgttgtt ggtaatcgaa
    39061 ctgtaaaagg aataaaaatt ccgaccgatg aaatcactac aaaacttttt gaacaaaata
    39121 attttacaca tatagaaaca attgtaagga atattccaaa taaacgaatg ccatcaaaaa
    39181 atagcccttc aaatattgct ggtaaaacag cttcgacaat gaagaacgag tacattgtta
    39241 tttgtcaaaa aaaataatat gcaattaaaa gaaattatct ctcgccttaa agaattaaaa
    39301 gccaaggggt ttattccatc gccaagaaaa ggtccaaccg gtgttggcta ttgttttgaa
    39361 caagaattag gagtgagcga atctaatatt ccgatccctg atgttggagg acgggtggaa
    39421 ttaaaaggaa caagaaagaa aacaaattca cttatcacgc tttttacttt taatagagca
    39481 gtatggatta taaagcaaaa agacctgatt gatacttatg gatataaaga tgcaaaaaac
    39541 aggcaagcgc tttacagtac agtaaataat aaatctccta atgctcaagg gctttacata
    39601 gaacttgata tggataaaaa tcttgtcatt ttaagacaca aagatgctga atttagtctt
    39661 gccgaatgga gtatttatgt tttagctgga aaatttatga caaaaatgga cagaattatt
    39721 cttggttttg ctgacacaaa agagattaat ggtaaagaat ttttccattt tactgatgct
    39781 gttttacttg aacaccccac acccgaaatg tttttggaag cctttagtaa aggtgaaatt
    39841 ttacttgatt taaggatgca tttaaaagag agtggagcag ttcgaaatca tggaactggt
    39901 tttaggattt cagagaaaaa tctgacaatg ctttattcga aacagaaaaa attgctataa
    39961 caagtatgtt ttcatgatgg tcataaaagc ttttgctttc gttcgctaaa gtctcttcac
    40021 cttgtctttt tatctattga ttagtagata tatctttttc atcattaata taatcagggc
    40081 aatctttaca gtcgaggctt ctgttataca ttagcattgc ccgcgagctc attcccatgc
    40141 ttgaaaagcc tccgtcatga aaaaggtttt gcattgtaac tttttttgtc agatcggaga
    40201 aaagtgttat gacataatcg gcgcactcgt cggctgaagc attacccaat ggcgacatgc
    40261 gttcggtgaa atccatcagc ccgtcaaagc cttttacggc gctgccggca gttgttttgg
    40321 ttggcgattg cgaaacggta tttaccctta ctttacgctc tcgaccatat atgtatccaa
    40381 agcttcgtgc tattgattcg agaagcgctt tggcatcggc catatcgttg taacgataaa
    40441 gagtgcgttg tgcggcaacg tatgacat
//
LOCUS       DAOOAI010000006        37492 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_1424_length_37492_cov_5.539012, whole genome shotgun sequence.
ACCESSION   DAOOAI010000006 DAOOAI010000000
VERSION     DAOOAI010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 37492)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 37492)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37492
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1424_length_37492_cov_5.539012"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1057
                     /locus_tag="PK345_00940"
     CDS             <1..1057
                     /locus_tag="PK345_00940"
                     /inference="COORDINATES: protein motif:HMM:NF039936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33618.1"
                     /translation="NVPVTLNVGTSYPLALTVVADPPTICAGESSQLNAIASGGTGSY
                     TYLWTSDPQGFTSTEPNPVVSPQVSTTYNVEVSDGVNSLSGQTTIIVNTAPDQPATPQ
                     GIASICAGTYQSSFNTDGAANAFFYNWFIEPAEAGTIYGNGLAGTVTWNPEFIGTADI
                     TVLGVNDCGNGELSLPLTVTMNPLPVVDLGEDQTICAHTSITLDAGNPGATYLWSTGA
                     TTQTIVVDSTGIGIGAANFWVQVTDNNGCTGTDEITIDFKDCTGIYDMSSTWSFDVHP
                     NPSSGEIKLQLETQNRNPVTISIVNSLGKKVAVLNNVIVAPTYSVELNLAKYGSGIYF
                     LRIDGNDVNLSKKIIIQ"
     gene            1237..3459
                     /locus_tag="PK345_00945"
     CDS             1237..3459
                     /locus_tag="PK345_00945"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33619.1"
                     /translation="MKTYTKLIAIFFLLISTSAFGSESVNKKLSSPISSLPEKALVAD
                     ISDVFWSINGYVYLSEESATPFPGVTITFSGLGSTLTNENGYYIFDVPHNWSGTVTPT
                     FCGPSYGFQPEQRVYPPVKKNYTQQNYYAVQLNTFTISGAFLHFKSGEPLANTSISFT
                     NEMSVTTNENGYYSLTVDPCWSDTLKPISSDWNFIPDFKYYSSVTTDFENQDFEYRPK
                     SFGLPPGWVFQNTGTVHIVSVFSTSNPNICGVPLNQGDYIGVFYKGTDDELHCAGAGE
                     WTGTSNTGVFIYGDDAYTPEKDGFLYGEVMNWKIFTWTTDEKEYEVYPSYQKGGYLVS
                     DNKWYSGGLSIVNGLNVYLNQQIVIPEGWSGISGYVNPSPKPDYVSGNQIQFLLAPIS
                     DDLIIIQSLVAMYYPSQNIKTLINWNVNSGYKIKVNKDVTFELPGCPITDRTLSLTAN
                     WSIMPVKSECNVSVQALFSGIMNRVKVVKEIAGPNVFWPAMGISTLQVLEPGKAYMVT
                     LTLNSSVTFPECTNQKSDAQGANAIHINKTNWTDPIVSPSNHIIAIESEAVKGFGKGD
                     YLGAFTQDGTCAGLAEIKSLDENMSIALFGNDNTETYKTGFFENELITLKWYRTSTDE
                     IFDLIAEFDPSTGSENGRFTDNGLSIITKLNIVSGTGETNRHITPHIFPNPTTGYINL
                     IAPESTYTMKITDVLGNIIHENEYTGSNNVDLSHVRRGLYIVSLQGADFIKTEKLLLR
                     "
     gene            complement(3463..5127)
                     /gene="ispG"
                     /locus_tag="PK345_00950"
     CDS             complement(3463..5127)
                     /gene="ispG"
                     /locus_tag="PK345_00950"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: protein motif:HMM:NF016438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(E)-4-hydroxy-3-methylbut-2-enyl-diphosphate
                     synthase"
                     /protein_id="HPX33620.1"
                     /translation="MITCFPTSEVMVGKIGIGGNNPVRVQSMTNTDTLDTIATLDQIK
                     RLSDMGCEIVRLAVPGIREARHLSVIKDELHRAGYDIPLIADVHFTPEAALISAELVE
                     KVRINPGNYTDRKVGKVSFTDADFKESQERISEKILPLIEICKKNKTVLRIGSNYGSL
                     SERIVSRYGNTPEGMVEAALEFVRICRRNDFHDIVLSMKASSVRMMVYANRLLVKRMI
                     AEGMSYPIHLGVTEAGNGAEGRLKSAIGLGALLADGIGNTIRVSLTEKPENEIPAAKE
                     IIGYFDDLATDRPFDRDYYLNLPHSYQPEINDRNLLKSEEKKPLVINVRGDYGSLKPD
                     LQIDGDKTSVDFTYIKSSDSVDKGLFIPQRPIVFESTKQASIIEFRDFLKMKRELSSK
                     LPVILKKSYSQKDILIEAAIDFGPIFLDNLADGIWITGNSYSSAELVELSFNILQACS
                     VRIFKTEYIACPSCGRTLYDIQTVFDAIKRKTSHLKGLKIAVMGCIVNGPGEMADADY
                     GYIGMGLGKAALFKGKTAIVKGVPESEAMDRLIELIKENGDWIDAE"
     gene            complement(5382..6221)
                     /gene="prmA"
                     /locus_tag="PK345_00955"
     CDS             complement(5382..6221)
                     /gene="prmA"
                     /locus_tag="PK345_00955"
                     /inference="COORDINATES: protein motif:HMM:NF001785.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HPX33621.1"
                     /translation="MNYYCYTISFKAQPDLDFFEIVPAWLAEAGFDSFTEEGKSIKAY
                     VSEDLHKPKEIEKLLVWVNKRIPIVWRSELIKDRNWNREWENNFQPVMIDDRCFVRAP
                     FHTVDTSAEFDIVIMPKMSFGTAHHETTQMMLEFILEEDWRQKEILDMGCGTGVLAIL
                     AVKMGAKSGLAIDNDIWAFENAQENIARNKVPEITCKHGNKTLIEDKKFDSIFANINR
                     NILLQHIPFYAKALKKGGKLFLSGFLEEDINTLLNKSEEHNLHFLKKKTLNNWAAIVV
                     GFS"
     gene            complement(6223..7563)
                     /locus_tag="PK345_00960"
     CDS             complement(6223..7563)
                     /locus_tag="PK345_00960"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX33622.1"
                     /translation="MGKIKIIILREYLTRVKKKSFIVMTFLGPILMAGIWVLPFILAT
                     MNTDEKRIQILDDTGLFEDRFVDTKTMKFTKLSIDVEVAKANLLKSGDYGLVWIPKTE
                     LSVPSTAIFYSSGQASLEVTSYIKSIMKNEVESLKLEASGIDPEVIRSIKTNIVVNTI
                     KISESGDEEKSFTELQMGVGIFAGILIYIFIFLFGTQVMRGIMEEKTNRIVEVIISSV
                     KPFELMMGKIIGVAMVGLTQFALWIILTLGIVTVVQTTVLDMSKLTKSEQMFIGQGKA
                     LNKEQLIDISEQMDEGDTIKLNMIVEAVNSINYGVMIVAFLFFFIGGYLLYASLFAAI
                     GSAVDSETDSQQFMLPVTLPLILSMVMLGMIVNNPSGPIAFWFSIIPLTSPVVMMIRI
                     PFGVPLWEVALSAILLIAGFLMTTWLAAKIYRTGILMYGKKVNYQELWKWIKYQ"
     gene            complement(7570..8490)
                     /locus_tag="PK345_00965"
     CDS             complement(7570..8490)
                     /locus_tag="PK345_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPX33623.1"
                     /translation="METLRVNNVSKQFTGHRALHNVSISVPRGSIFGLLGPNGAGKTT
                     LIRIINRITAPDEGEILLNGRSLGSDEVMKIGYLPEERGLYKKMKVGEQAIYLAQLKG
                     LSKHEAIERLKVWFKKFEITTWWDKKVEELSKGMQQKVQFMVTVLHKPDLLIFDEPFT
                     GFDPINVNLLKDEILKLRDEGATIIFSTHNMASVEELCDYIALINQSEKILDGNVDEI
                     RRSFKQKIFEITFPTSAISFAEMLPDGYQLVEEISQNGMTIARIKIPVEDSPNILLNY
                     AIKNVEVHGFSEILPSMNDIFISTVNEFKK"
     gene            8982..10304
                     /locus_tag="PK345_00970"
     CDS             8982..10304
                     /locus_tag="PK345_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33624.1"
                     /translation="MKNIASLRMLLLPMLLFFIIGSGRSQETYTRPITTPWHWLSFPR
                     LKREANNPVLAIPVLENLDPIPDNLYFYSNERGHNKFIKRTGGNWNEIKFYYIYSTKG
                     YKLNILDVGSFTHTMYGTDLNPNTPIQLYAMQKNNSENWIGYFLHRPLVPEDAFQGVW
                     EYLTEIKTQYWTMAKIIINGRPTWLRPSNVTPIKYGDALVVKVSEDCTLYWNQTCEPA
                     GEILRVVPEYFDYIEYADYQPWFIQTDSLGDVKEIGLMAGDVCIGASTVKTGDTLVGV
                     NAYTQAVPPGTPITIATWDGLKSTAAITGKYFVIRPETGRKEYRTVHTGEGQPYYYIS
                     FTGQQNELQQNREQTAFLSGISPNPCKEAAFIRFNVLKEADVAIEISDINGRRISTVA
                     KGVYPEGSFEVVWKTTDAYGKPVENGIYIVSLAVDDRFIENQKLVVIR"
     gene            10315..12687
                     /locus_tag="PK345_00975"
     CDS             10315..12687
                     /locus_tag="PK345_00975"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HPX33625.1"
                     /translation="MKNLIITAILLASTISHAAVVTVKQDGTGDFTHIQQAINAAAVN
                     DTVLVWPGTYYENLSFYGKDLTLASLYLTTGKREYIAQTIIDGNKNGTVITLQDGETL
                     ATMICGFTIQNGKKDFNSSLWRKMGCGICLKQSHAVIKNNVIKQNQGFTGGGLSVLGG
                     FTKLYGNIITDNHSIFIGGGIYYNNSSVPIYLDTIELNSIFLNYSNWGSDIIVRSIGQ
                     QQIIEIDTMTVFDPDCYFALSIDGYSNLINDLTLNIRHQKIEPVAADLYVSPQGNNNN
                     SGLNPENPLKSISFAITKIQPDSLVQRTIHILPGTYSPQTTGERLPIGGRSNIKLSGE
                     DMSNTIIDCQYEGCFYSGRYMRNIELESFTIKNGFGAILTPQGVFDAGLDIDFTKKVK
                     ISNITIDSVINAESQCISCHEVDSIEIKNFHCRNSIGVQANFFFRGGENFPMYVRIEN
                     AKIHGNTYYDEGTFLFGGYKNLIVSGGDLNNEICTGKFINVEITDNISWDTWYEHTCG
                     PGWATSEKVKLDIVNATIGGNRDRMNLPGTNSTVKSSEVNIYNSIFYDNNSPDIYIYW
                     ANETKPAEINFHYTLFENGAAGIIDSAGNTIVNWGEGILDKDPQWQGYGANPYALTIN
                     SPCINAGTPMYEPGMSPPYIKEENERYILYTPNMDTIHLPATDLIGNPRIAYGRIDMG
                     AYEFADITINIPSPAKLIGGEIKVFPNPFQSSTNLQFTLLKGGQCEALIYDLNGRKVK
                     TLMNAFTPPGNYRMRWHGDSDYGNDAPPGIYIIAIQIDGQQAGSVKVERR"
     gene            complement(12952..13794)
                     /locus_tag="PK345_00980"
     CDS             complement(12952..13794)
                     /locus_tag="PK345_00980"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HPX33626.1"
                     /translation="MKSIVKVFLIINILVLSACERMITDVDIDPGRKKLVVHSYLYPG
                     MDTVKVMLTSSLLLYSSTELCQGPSNNEVDDAQIVIRNKNIEYPLTYNDSIKQYISTE
                     LEIKKGETYNLYVKASEGEELFASCTVPGENPPDIEILSIQSDTYGGKIINFRFRDLN
                     GQGHYYRICGVYKYFYAPEPESFHLYSIYMDKGEEFVTDINKDGEYFIYKTNWIYLDE
                     EPNPVTLFLALTDIHYYNYHRSVFNFDGENPFSEPSPVYSNIEGGLGVFAAFNGSTIE
                     VDLE"
     gene            complement(13799..16162)
                     /locus_tag="PK345_00985"
     CDS             complement(13799..16162)
                     /locus_tag="PK345_00985"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPX33627.1"
                     /translation="MRKTSVCLILFFLLFSIFSAAQSRFTISGFVRERGSQELLPGVN
                     IYLPEQRAGTTTNNYGFYSLSLPVGSYRIQYSYVGYQLVEEQVNLNKHIEIDVELEAN
                     LTLSEVVVSAEKYRRLTESDQMSMISLPVIQIKNIPALLGEKDVFKVLQLMPGVQKGS
                     EGSSGLYVRGGCPDQNLIILDDAPVYNVSHLFGFFSVFNGDAIKSIELTKGGFSARHG
                     GRLSSVIEMTMKDGNKEKISGQGGIGLISSRLTLEGPLVKGKSSFLVSGRRTYIDLLI
                     APYMPQDERGGYYFYDLNTKLNYDFGPKNKLYLSGYFGRDKFYFVSKYSNSKDKGALY
                     WQNKTGTIRWNHLFNNRVFSNTSLIYSEYNLNIYEEYRSSNDKYELKYTSGIYDVGLK
                     YDLEYHHSPEYSLRTGMQSTHHYFLPSAIVEIDDNVSHEFKSKTDYTSFESAVYVENH
                     FNFKNIIQVNAGFRMAHFIADKNHYFSPEPRLSVNYLFGKNISFKAAYSVMNQYIHLL
                     SNTGIGLPTDLWVPSTDRIAPQHSSQISAGIAKELVEKGIQVSLEGYFKKSKNILGYK
                     PGSSFLLIDDPTEVQSYTWQDNVTAGRGESYGAELLLHKKEGRTSGWIGYTLSWTQLQ
                     FDEINFGKKYWARYDRRHDIGIVVIHELNKRITLSGSWVYGTGNAVTLPLGSYYSQSH
                     DPFESMRDSPFYHYYSWSEIYDYGEINSFRMKPYHRLDIAVQFHKKMKKHERIWELGL
                     YNAYNRLNPFFYFISREYDWETNTETSKLKQVSIFPIIPSVTYNFKF"
     gene            16849..17811
                     /locus_tag="PK345_00990"
     CDS             16849..17811
                     /locus_tag="PK345_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018965882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPX33628.1"
                     /translation="MKNILVIGSVGQIGSELTLELRKRYGNNNVVAGYRKTQPSVELA
                     ESGPLEVVDATDIVRLAEVVKKYKIDTIYNLAALLSAVAEKNPQAAWNVGVNGAYNVL
                     EVAREYKCAVFFPSSIGAFGLSTPLDNTPQDTIQRPGTMYGVTKVAAELLSDYYFKRF
                     GVDTRGVRFPGIISNVTLPGGGTTDYAVEIYYAAIKDGKYTCPLRKGTFLDMMYMPDA
                     LDAAIDLMEADPSKLKHRNCFNITAMSFDPDIIYNSIKKYIPNFKMDYDVDPVKQGIA
                     ESWPNKMDDSAAREEWGWNPKWDLDKMTQDMLKVIKEKHDKGLI"
     gene            complement(17989..19068)
                     /gene="hydE"
                     /locus_tag="PK345_00995"
     CDS             complement(17989..19068)
                     /gene="hydE"
                     /locus_tag="PK345_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydE"
                     /protein_id="HPX33629.1"
                     /translation="MSTLKNLLDKSSLSREDIILLLQAEGEAMQDLFRKATEVKRFYV
                     QDKVYFRGLIEFSNICSKNCYYCGIRSANRKTERYNLSDDEIVDAAIFAYENGYGSIV
                     LQSGELSNKRFVNRISNLLSKIHGATNQKLRITLSCGEQERDTYQKWFDLGAIRYLLR
                     IETSNRDLFYKLHPNNRLHDFDNRLKCLEYLKDVGYQVGTGVMIGLPYQTYDDLAGDL
                     LWMKSIDVDMVGMGPYIEHHDTPLYDLRHLLLPIGKRFELSLKMIALLRILMKDINIA
                     AATALQAIDKTGREKAVEIGANVIMPNITPYSYRDQYKLYENKPMTTDSPDDFLARLE
                     ERIKLAKNEIAYNEWGDSKHWEEKQ"
     gene            complement(19093..19887)
                     /locus_tag="PK345_01000"
     CDS             complement(19093..19887)
                     /locus_tag="PK345_01000"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004799 - thymidylate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HPX33630.1"
                     /translation="MRQYLDLLEHVLDNGVKKEDRTGTGTLSIFGYQMRFDLDEGFPL
                     LTTKKLHLRSIIHELLWFLKGDTNIKYLHDNNVTIWDEWADGEGNLGPIYGFQWRSWP
                     TADGRYIDQISQVIHSIKENPHSRRHLVNAWNVGEIEKMALPPCHILFHFYVANNKLS
                     CQMYQRSADIFLGVPFNIASYALLLMMFAQVTGYKPGEFIHTFGDAHIYLNHIEQVKL
                     QLTRNPFQLPKMEINPLIKNIFDFKFEDFVLKNYQSHPHIKGEISV"
     gene            complement(19941..21149)
                     /locus_tag="PK345_01005"
     CDS             complement(19941..21149)
                     /locus_tag="PK345_01005"
                     /EC_number="3.4.13.19"
                     /inference="COORDINATES: protein motif:HMM:NF013413.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070573 - metallodipeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPX33631.1"
                     /translation="MKYFSFFFAFLLVGCTTTSADKKLLEEALRIHSKAITIDTHTDT
                     PLYFIHAGFDFGQRHDARQEGSKVDIPRMEEGGLDALFLAVFIVQGERSAAGNARAFE
                     RADLIFDSIVSVLKRNNDKAEIAFNASDIERLKYSGKRAFLLGIENGYPIGNDLSLIK
                     YFYDRGARYITLCHSKNNDICDSSTDTVEFNGLSAFGEAVVKEMNRIGMMIDVSHISD
                     SAFFDVVRLSTTPVIASHSSARAICNNPRNFSDEMLKALAKNRGVIQVCLLSDYVKTF
                     PPNLQRDSVMNELLIKYRYFKDLSDEESAMASAEWQAMNLKYPIELATVSDYVDHIDH
                     IVKTIGIDHVGIGSDFDGGGGLADCIDVGEFPKITMELLRRGYVEEDIIKIWGGNFLR
                     VMEEIQNKAG"
     gene            21465..21668
                     /locus_tag="PK345_01010"
     CDS             21465..21668
                     /locus_tag="PK345_01010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33632.1"
                     /translation="MTVHPALRQTINDSVSYSDFLQNLKSTALSFLSMNSAIGALLSA
                     GWPVTNQQIFITAAARRGQYAKL"
     gene            21765..22118
                     /locus_tag="PK345_01015"
     CDS             21765..22118
                     /locus_tag="PK345_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33633.1"
                     /translation="MQKSAKEKIIGRLSENKSVFLAQQLSDGKGRVDKIRRHSPIIKQ
                     MGKEVIVESKSVAEFIRQMMRMGEDGRGYETLYGYSIGITEKRFPLYRCPKYDFEITD
                     ILTNLKLENINRTNR"
     gene            22115..22957
                     /gene="deoC"
                     /locus_tag="PK345_01020"
     CDS             22115..22957
                     /gene="deoC"
                     /locus_tag="PK345_01020"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HPX33634.1"
                     /translation="MIDFDNYNFTLSQLSDRANRIKSIPLNDSLKMEYYRKILNLVDL
                     TTLEGSDTPEKIETLCRYALRLQSDNPNNGTVAAVCIYMPFVELAVGLLQNSPLEVAT
                     VAGGFPSGQLPVHLKKQEIEYVVQQGANEVDVVISRSLALMEKWNELQEEIIQMKASC
                     GETKMKVILETGELTEVSKIRKACECAINGGADFLKTSTGKIKPAATPEAFLIILDTI
                     NEYRQKTGKMIGVKPAGGIATPDDALHYYLMVKETLGNEWLDKKWFRIGASRLVGNLL
                     ERMK"
     gene            23124..24635
                     /locus_tag="PK345_01025"
     CDS             23124..24635
                     /locus_tag="PK345_01025"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HPX33635.1"
                     /translation="MKTKIQILTGIILAVFFIFKNEDVLAQKIKGNMEVVSQKRIVDE
                     FTGIEAGSFFDIYLTMKDEQSVLVETDSNLQEEVKTLVTDNILKIKASNLKNTSKVKV
                     YIAVPEINYINLSNAARVEGTNKIEASALEIIATGASQIKLDINVSELILNVSGASDV
                     KLTGNAGNSEITTSGAANLVAKALKTNTANIKTSGASNVIIFVNENANTSASGASDIK
                     ILGEPKIEIKDNDEGTSRAKIFAVEEDEDYTRITAAGIDIEVVDGDSTMVKVGNKSLV
                     VDKKGNVKYTGDKKSKKKFNGHWAGLDIGINGFVDKDFNTGKPKGYEFLNLNYEKSID
                     FSINFFEQNFNLIKNKFGIVTGAGLRVSNFRFEDQRLILVNDSARIFGFSDPLVKWQK
                     SKVVNNYLTVPLMLEYQTNPNSNRNSFHLSAGMIFGWKYRTYTKMMEKENGRNVVKNR
                     ETFHMSPFRYDLSGRIGWGVLNLYATYSLNTLFKKDEGPKLYPFAIGITITGW"
     gene            24860..25618
                     /locus_tag="PK345_01030"
     CDS             24860..25618
                     /locus_tag="PK345_01030"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPX33636.1"
                     /translation="MNQSEKSKILSSFYYPALIVGFMWLVKVLEMAFGVQLYRFGILP
                     LTCKGIPGILTSPFIHSNLAHITSNSLPLLVLGSLLFYIYRTIAWRVLIFIWIITGFW
                     VWILGRDSFHIGASGIVYGLAFFLFFSGIFRKDSRLLAITFLVAFLYGSMVWGIFPDL
                     FPKKNISWESHLMGMIAGIVLAFFYRNEGGPARKIYSWEIEEETDSDDDDPDAYWKKE
                     SVEKHKDKPDSPTKPLQINYIYKKSSKEENNNNT"
     gene            25635..26822
                     /locus_tag="PK345_01035"
     CDS             25635..26822
                     /locus_tag="PK345_01035"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HPX33637.1"
                     /translation="MREVYIVSAVRTPIGSFGGSLSSLSATQLGAIVIKAAIEKAGVA
                     PNDVDEVLMGNVLSANLGQAPARQAALFAGLPDSVHCTTLNKVCSSGLKSVMFGAQSI
                     LLGDNDVVVAGGMESMSIVPHYITAVRRGMKLGHSQLVDGMIKDGLWDVYNDYHMGNA
                     ADNTAKVKGITRDEQDRLAIESYKRAAKAVAEGRFTSEIVPVESQSKKGTVLVKEDEE
                     YRNVNFDKMLELKPVFNKEGTVTAANASTINDGAAALVLMSKEKAVALGIKPLAKIIG
                     YSDAANAPEWFTTAPAKAIPKAVAKSGLNLEQIDFFEINEAFAVVSLAVMKELNLDRE
                     KVNIWGSGISLGHPLGATGARILVTLVNILQQNNARYGVAGLCNGGGGASAVVIENLR
                     ISK"
     gene            27199..27468
                     /locus_tag="PK345_01040"
     CDS             27199..27468
                     /locus_tag="PK345_01040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33638.1"
                     /translation="MKKNSKSYIINCPIHQKQRSLVIEAKRKINHSNRRVEKTIYAED
                     VQFLAKQLLECDQFEANKEDCKQCHQFATTQHQSVEGVLKVITIG"
     gene            complement(27483..29540)
                     /locus_tag="PK345_01045"
     CDS             complement(27483..29540)
                     /locus_tag="PK345_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007762185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HPX33639.1"
                     /translation="MVNKELINPKSIVVVGGSNDTSKPGGKILKNLIDNGFKGKLYVS
                     NPKETEVQGIRSFQNTDDLPQVDLAIIAIAAKYTLSVVEALAYNKGTKAFIIISAGFG
                     EESDAGRRLEEKIVEVVNSVQGSLIGPNCIGVITPAHASIFTLPIPKPDPKGVDFISG
                     SGSTAVFIMESAIPKGLTFNNVFSVGNSAQMGVEEILEYMDVSFDSETSSKVKLLYIE
                     TITKPQKLLKHASSLIRKGCRIAAIKAGSSEAGSRAASSHTGALASSDVAVDALFKKA
                     GILRCYGREDLTYTASVFMNKQLPGPNIGIITHAGGPAVMLTDVLSNGGMKVPHINHP
                     ATKELLDKLYYGSSVANPIDFLATGTVEQFGLIIDYCDKTFDEIDAMVVIFGTPGLIK
                     VFDAYRVLNEKMKSCRKPIFPVLPSVLTASDEVAEFLSFGRVNFPDEALLGEALCRVY
                     HNPAPAPVHVDLPDVDHQSIRRIINSSDDGYLAPEKVQVLLDSAGIPRVGEGVFTNKH
                     EAVEGAEKLGFPVVMKVVGPVHKSDVNGVVLNVEDAEQVEAEFDRMIKIPDTTAILIQ
                     PMISGIELFAGAKFEPKFGHLILCGMGGIFIEVLKDVSTGLAPLSTDEALQMIRGLKS
                     YKIIQGIRGRKGINENQFVEILRRLSALVSAAPEIMELDLNPLLGNADRITAVDARIR
                     IEK"
     gene            29655..30725
                     /locus_tag="PK345_01050"
     CDS             29655..30725
                     /locus_tag="PK345_01050"
                     /inference="COORDINATES: protein motif:HMM:NF040322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33640.1"
                     /translation="MKRLYKLTRQILPTLLFLLSAIIIAQEANVENRAIQNYRVMFYN
                     LENLFDIYDDPDTHDDEFTPTGSRRWTNKKFYAKLNNIYKVVMAVGGYEPPTVVGMCE
                     VENEFVLQRLLNNTPLKQFGYKFIHYDSPDSRGIDVAMIYRDDKFTPFHSEAIPVIFP
                     DDPDSKTRDILYVKGLIGGREMIHIFINHWPSRYGGYMETKPKRGRAAEILKHKTDSL
                     FNINPSAVIVIMGDFNDGPNDESLFKTLDAKNPENDIQTTALYNMMLVDRKGWEHGTL
                     KYREYWDTFDQIIISGSLLDKTGNLWVDPNQEVIFHSDFLLQPDERYLGKKLIRTYLG
                     FRYIGGYSDHLPVFFDLKMKME"
     gene            30869..33124
                     /locus_tag="PK345_01055"
     CDS             30869..33124
                     /locus_tag="PK345_01055"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPX33641.1"
                     /translation="MFENKDNLNSSESESDGNSLAGHMSAFFNRNHENNLSLTNLPVP
                     LLIVDESGTVLNCNQEALEFLNCSEDELNFSLFEMIPPDYQPYTTTLLDSAKTTGKPQ
                     MGEMILVLKDGKETRVKIFINLYVRKKEDVLFTIVIADLSDVKQFYEEKLFEIELKYR
                     ELAESINEGIYMTEWSTITKVNTPLLKIFGYSESEVIGRRVWEFVVPEYREKIKKISL
                     QKIKQMDNSPVEVQVLRKNGTRFWAEVRINIFKEEHKIFGVLSDITNRKAVEKALKES
                     ELKYRSVVDTMNEGVVMRDISGKVVTWNQAAERILDMTSEEMPFFNILAPHWKAVKED
                     GSAFDINEHPAVISLSTGKAVQNVIMGIHTKTGTFRWIRTNSAPVMNNNKGTIKAAVS
                     SFSDITYLKTTENKLREINSIKDKLFSIIAHDLKSPYNAQMGFLELLMDADQHHTPEQ
                     RKQFIQMLYESTRQSFALLDNLLVWSRNQTGKTPFQPKEFDIVEMIDNAVKIFELSAA
                     VKKIAIKTKIAQSNLIVRADYEMTNTVLHNLLSNAIKFTPSKGTISISCKPAKRNQLT
                     VSVKDTGIGMSEEFVEKLFVSDDIHSTPGTENEKGTGLGLFICKEFVERNGGRIWVKS
                     ALGKGTTFFFTIGSAGKHEPGQCKCILNIEDVYNEIIKNPEVTARFYNDVAFAFYQSF
                     KTFKNKDFQVFSNKLNKLIDQYQLESLSQFAKCFNQNYSTCEKNQLNICFRELEKLFD
                     KLNIMPIINPR"
     gene            complement(33169..35292)
                     /locus_tag="PK345_01060"
     CDS             complement(33169..35292)
                     /locus_tag="PK345_01060"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HPX33642.1"
                     /translation="MKFWNSYPFFRLIIPFGIGILSAIYLPVPHFIQPLWLIIITLLL
                     LFLTIFTHYIKTYSLRWLPGLFLNVFVLSTGYTLTLVQTDLYRSNHFSHVTDGSELMV
                     VRISEQPSERARSVRAFGKVVMLKDSINEFPTIGKILIYLEKDSVSQTLKYGDIIVAK
                     AIYNHVPSPSNPHQFDYQRYLSHSGIYHQTWLGSNDWQRIKKGQVNPIFSLSYKARDI
                     MLRTLEEQGLDGNEFAVVAAILLGYDDRMEPELRNHYAGAGALHVLCVSGLHVGIIFM
                     IVSVLLKKLTSKSGRIIKFFILLGSIWMYAFITGLAPSVLRASVMFSLFTWRELIGKK
                     SNSYNILAASAFILLAYDPYLITKVGFQLSYSAVIGIISLYDLVYRQLIFKNAVLDYF
                     WKLAAISIVAQIGTSPLAIFYFNQFPLYFLLANIVVIPVVWLIVYSGIATLIVAIVSS
                     KLSAWIAKVLYYEVYFLNMAVEWVNKLPQARVEGLVLNFAQVIIIYLVIIFLVRFLVA
                     KEGKYGVITLLLLLIFTASITFQKMKVIEQNKIIVYNVKGYSAIDLVMGQDLISIADS
                     SLLLDQKTVDFNIKNARIYSGIHKQKQLIFEEVMSVEDTQIDGNLKVYANGVIQAFDK
                     VIAILNDKFQPFFSDKPLQVDWVILQKNCPIPLDQIRKMFTFERVIFDSSNTPWRARQ
                     WHRYCTDNQVPFFDVSQSGALVVTL"
     gene            complement(35361..36128)
                     /gene="yaaA"
                     /locus_tag="PK345_01065"
     CDS             complement(35361..36128)
                     /gene="yaaA"
                     /locus_tag="PK345_01065"
                     /inference="COORDINATES: protein motif:HMM:NF002542.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HPX33643.1"
                     /translation="MHILLSPSKTLNEQVKIPSLDFTQPHFLKQAAQLVEILKKFSPS
                     QIQLLMNVNPKLADLNALRYLEWGLPFTIENALPAVLMFKGEVYNGLKAETLTDDDLE
                     FAQEHLRILSGLYGLLRPLDLIKPYRLEMGAKLKNKRGDDLYQYWGDSITNMINEQLK
                     ERKAKCIINLASDEYFRAIKMERLEAEVITPQFKDFSGGSYKFITVYGKKARGMMARY
                     IITNRLENVDEIKLFDSEGYHFNQSFSDKSTWVFTRG"
     gene            complement(36186..36857)
                     /locus_tag="PK345_01070"
     CDS             complement(36186..36857)
                     /locus_tag="PK345_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33644.1"
                     /translation="MKRVYFILILFLGLHLAAQEPNHNSFPAVTSEDSVTIRSFRPGL
                     LPFNFDKKLSFRLEVGSAFGFSSSGGQMFGVFTSPHFSYKLSDKWRINSGLRIQNSNF
                     LSWYNPFIPHFPERMQIFDHNITQTLMYAESEYLINPRLLISTKVFKEVSLFNEPQVN
                     PYALDLSGEGVSFGINYLVNDNFHFGAEVGISRGVNPYNPWYPATFGYPGRSPYKINS
                     LSPFD"
     gene            37148..>37492
                     /locus_tag="PK345_01075"
     CDS             37148..>37492
                     /locus_tag="PK345_01075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33645.1"
                     /translation="MTSVEILVPISLFAMIALIVYIQARKKIHLTLIQHGKDAKLLKT
                     EKDANISLKIGLLMVGISLGILIGNILVAKKYMAEEPAYFSMILICGGVSLILYYFLI
                     NREAKKGIKKKHG"
BASE COUNT        11877 a         7427 c         7232 g        10956 t
ORIGIN      
        1 aaatgttccc gttacactta atgttggcac atcttatccg cttgccctaa ccgttgttgc
       61 tgatccgcca acaatatgcg cgggagaaag ctcgcagctc aacgctattg cttcaggcgg
      121 aaccggcagc tatacttatc tgtggacttc cgatcctcag ggatttactt caacagagcc
      181 taacccggtt gtatcgcctc aggtttctac aacctacaat gttgaggtga gcgatggagt
      241 aaactctttg agtgggcaaa caacgataat tgttaatacg gcgcctgatc agccagcaac
      301 accacaaggg atcgcttcca tttgtgcagg aacgtaccag tcgtcattta atactgacgg
      361 tgcggcaaat gccttctttt ataattggtt tattgaaccg gcagaagccg gaaccattta
      421 tggaaatggt ctggccggaa ccgttacatg gaatcctgaa tttattggaa cagcagatat
      481 aaccgtattg ggagtaaatg actgtggtaa cggagaactc tcactgccgc tcacagttac
      541 aatgaaccca cttccggtgg ttgatcttgg cgaggatcag acaatttgcg cccacacttc
      601 tattaccctt gatgccggta atccaggagc tacttatctg tggtcaacgg gagctacaac
      661 ccaaactata gtggttgatt ctaccggaat aggaataggc gctgctaact tctgggtgca
      721 ggttaccgat aacaacggat gtaccggcac agatgagata accattgatt ttaaagactg
      781 cactggaatc tacgatatgt caagcacatg gtcgtttgat gttcatccca acccttcaag
      841 cggagaaata aaacttcaac tcgaaacgca aaacagaaat ccggttacaa taagcattgt
      901 taactcatta ggaaaaaaag ttgcagtttt gaataatgtc atcgtggcgc ccacctattc
      961 cgtagaacta aaccttgcca aatacggcag cggaatctat tttttacgca tagatggtaa
     1021 cgatgtaaac ctgagcaaaa agataattat ccaataatca ttaataagtt tttttctttt
     1081 ttttaccaaa cggttacaat gacttttgct ttgtaaccgt tttttttatc ctaacacgcc
     1141 atattttatt ttaattagtt ttgctcaaaa tattaatcat actaaaaaga atcttcttca
     1201 ttaattgtta ctttttgaca tcataacata aaacccatga agacctatac gaaactcatc
     1261 gccatatttt ttttactaat atccacttcg gctttcggat cggaaagtgt gaataaaaaa
     1321 ttatcatctc caatatcttc gttaccggaa aaggctcttg tggctgatat atcagatgtt
     1381 ttttggagta tcaacggata tgtttacctt agtgaggaat ctgcaacccc tttccccggt
     1441 gtaacgatta cttttagtgg attagggtca acgttaacca atgaaaacgg atattacata
     1501 tttgatgttc ctcataactg gagtggaaca gtaaccccta ccttttgcgg cccatcatat
     1561 ggatttcaac ctgagcaacg ggtttatcct cccgtcaaaa aaaattatac acagcaaaat
     1621 tattatgctg ttcaattaaa tactttcacc atttcggggg cttttcttca ttttaagtct
     1681 ggcgagccgc ttgcaaatac atctatcagt ttcactaatg agatgagtgt taccaccaat
     1741 gaaaacggat attattcttt aacggttgac ccgtgctgga gcgatacgct taaaccgata
     1801 tcatctgatt ggaactttat tcccgatttt aaatattatt caagtgtaac cacagatttt
     1861 gaaaatcagg attttgaata taggcctaaa tcttttggat taccgccagg gtgggttttt
     1921 caaaacaccg gaacagtaca tattgtttca gttttttcta cttcaaatcc aaatatctgt
     1981 ggagttcctc tcaaccaagg tgattatatt ggagtttttt acaaaggaac cgacgatgag
     2041 ttacattgtg ccggagcagg cgagtggaca ggaacttcaa acacaggggt attcatttat
     2101 ggagatgatg cctacacacc tgaaaaggat gggtttttat atggcgaggt aatgaactgg
     2161 aaaatattta catggaccac cgatgaaaaa gagtatgagg tttacccaag ttatcaaaaa
     2221 ggaggttacc tcgtgtccga caacaaatgg tatagtggag ggctctcgat agtaaacggc
     2281 ctaaatgtat atctaaatca acaaatagtt ataccggaag gatggagtgg gatatcagga
     2341 tatgttaacc cgtcgccaaa gccagattac gtgtcaggaa accaaataca gtttttatta
     2401 gccccaatct cggatgatct gataataatt cagagtcttg ttgcaatgta ctatccttca
     2461 caaaatatta aaaccctgat taactggaat gtaaattcgg gttacaagat taaggttaac
     2521 aaagatgtaa catttgagct acccggctgc cctattaccg accgaacact ttcattaact
     2581 gcaaactgga gtattatgcc ggtgaaaagc gagtgcaacg tctcggtaca agcactcttt
     2641 tccggtatta tgaacagagt taaggttgtt aaagagatag ccggccctaa cgtcttttgg
     2701 ccggcaatgg ggattagtac acttcaagta ttagaaccgg gaaaggcata catggttaca
     2761 ttaaccctaa attcatcagt taccttccct gagtgtacca atcagaaatc agatgcccaa
     2821 ggagctaacg ccattcatat taacaaaaca aattggaccg acccgatcgt ttcaccatca
     2881 aatcatataa tagccataga gagcgaggcg gttaaaggat ttggaaaagg tgattatctg
     2941 ggggctttca ctcaagatgg aacctgtgca gggcttgctg agataaagtc gttagacgaa
     3001 aacatgtcaa tcgcactttt cggaaacgac aatacggaaa cctacaaaac cggctttttt
     3061 gaaaacgaac tgataacttt aaaatggtat agaacctcaa cagatgaaat ttttgacctt
     3121 atagccgagt ttgatccctc aacaggctct gaaaatggaa gatttacgga taacggcctc
     3181 tccatcatta cgaaactgaa catcgtttcg ggaacaggag aaacaaaccg ccatataacc
     3241 cctcatatat ttcccaatcc gaccaccgga tacattaatt tgatagcgcc ggaatcaacc
     3301 tatacaatga agataactga cgtgcttgga aacataatcc acgaaaatga atataccggt
     3361 agcaataatg ttgacctaag ccatgtgcgg agaggccttt atatagtgag ccttcaaggc
     3421 gccgatttca tcaaaaccga aaaacttcta ctcaggtagt catcattcgg cgtcaatcca
     3481 atctccattc tcttttatca attcaataag cctatccatc gcttcggatt cgggcacgcc
     3541 ttttacaata gccgtttttc ctttaaaaag tgccgccttg cccaatccca ttcctatgta
     3601 accataatct gcatcagcca tttcgccagg cccgtttaca atacaaccca tcacagcgat
     3661 cttcaaacct ttcaaatgcg aagtcttccg tttaatagca tcaaacacag tttgaatatc
     3721 atataatgtt cgcccgcacg aggggcaggc aatatactca gtcttgaaaa tcctgacgct
     3781 gcaagcctgt aaaatattga aagagagctc aaccaactca gccgaagaat aagagttacc
     3841 tgtaatccaa ataccatcgg ctaagttatc aagaaatatc ggcccaaaat caattgcagc
     3901 ttcaatcaga atatcttttt gcgaataact ctttttcaag ataacaggta atttagagct
     3961 tagttctctt ttcattttca gaaaatcacg aaactcaatg atacttgcct gttttgtgga
     4021 ttcaaaaact atcggccgct gtggaatgaa aagcccctta tcaacgctat cagagctttt
     4081 gatatatgta aaatcaacag aagttttatc accatctatc tgcaaatccg gcttgagaga
     4141 accataatct ccacgcacat taattaccaa tggcttcttc tcctcgctct ttagcaaatt
     4201 gcgatcatta atctctggct gatatgaatg cggcaagtta agatagtaat ctctatcaaa
     4261 aggtctatca gtcgcaagat catcgaaata gccaattatc tctttcgctg ccggaatttc
     4321 attttccggc ttttcggtaa gcgacacacg aatggtgttg ccgatcccat cggctaataa
     4381 tgcacctaag cctattgcag acttcaacct gccttctgcg ccgttgcccg cttcagttac
     4441 tccaagatga ataggatagc tcatcccttc tgcgatcatc cgtttcacta acagcctgtt
     4501 ggcataaacc atcatcctta cgctgctggc tttcatagag agaacaatat catgaaaatc
     4561 attgcggcga caaatacgta caaattccaa agcagcctcc accatacctt cgggcgtatt
     4621 gccatatcgc gaaacgatcc tttcggataa agagccatag ttggaaccga tccttaaaac
     4681 ggttttgttc tttttacata tctcaattag cggcaaaatc ttttcggaga tcctttcttg
     4741 cgattcctta aaatcggcat cggtaaaaga gaccttgcca acttttctgt cggtataatt
     4801 gcccgggttg atcctcacct tttccactaa ctctgccgaa atcaatgcag cttcaggggt
     4861 gaaatggaca tcggcaatta gggggatatc atatcctgca cggtgtaatt catcttttat
     4921 tacactaaga tgccttgctt cccgtatgcc gggaactgcc aacctgacta tctcgcaacc
     4981 catatcggat aagcgtttga tctgatctaa agtagcgata gtgtcgagtg tatcagtatt
     5041 ggtcattgac tggactcgta ccggattatt tccgccaatc ccaattttgc caaccattac
     5101 ctccgatgtg ggaaaacaag tgatcattgt cgtatttttt agcaaaataa acctaaatta
     5161 atatttgacg atacttattc tgagtttacc aataatgatt ttgatgctaa aattattatt
     5221 ttaagcggat acaacgaaca aacgccaaat aataaatggt tactcctaaa actaataatt
     5281 tgtcgaataa tggctcccta ttgcataatt cgggattaat tagcctcgta agatcaataa
     5341 ttcggcggaa aaaggttttt atggttttgt tcgggggatg gctaactaaa tcctacaaca
     5401 atagctgccc aattattcaa ggttttcttt ttcagaaaat gcaggttgtg ctcctccgac
     5461 ttgttcaaga gcgtattgat atcctcttcc agaaagccgc taaggaaaag cttgcctccc
     5521 ttttttaatg cttttgcgta aaagggtata tgctgcagta agatattacg atttatgttg
     5581 gcgaagatag aatcaaactt tttgtcttca atcaatgttt tgttaccatg cttacaggta
     5641 atttccggca ctttgttgcg cgcaatattc tcctgtgcat tttcgaatgc ccagatgtcg
     5701 ttatctattg ccaatccgct tttcgctccc atttttacgg ctaaaattgc taatactccc
     5761 gtaccgcagc ccatgtcgag gatctctttt tgtcgccaat cctcttccaa aataaactct
     5821 aacatcatct gtgtcgtttc atggtgcgct gttccgaacg acatctttgg cataatcaca
     5881 atgtcgaatt cggcagaggt atcaacagta tgaaatggcg ctctgacaaa acaacgatcg
     5941 tcaatcatca ccggctggaa attattttcc cattcccgat tccagtttcg atcctttatc
     6001 agttcactcc tccatacgat aggtattcgc ttattgaccc aaaccaacag cttttcaatc
     6061 tctttcggtt tatgcagatc ttccgaaaca taagccttta tactctttcc ctcttcggtg
     6121 aagctatcga aaccggcttc tgcaagccat gccggtacaa tctcaaaaaa atctaagtcg
     6181 ggctgggctt taaaggaaat tgtataacaa taataattca tcctactgat atttaatcca
     6241 tttccataac tcctggtaat tcaccttttt accatacatc aatatgccgg ttctataaat
     6301 ttttgccgct aaccaagtgg tcattaaaaa gcctgcgatc agcaaaatag ctgataatgc
     6361 cacttcccat aatggtacgc caaaaggaat acgtatcatc ataacaaccg gcgaggtgag
     6421 cggaatgata gaaaaccaaa atgcaatagg gcctgagggg ttatttacga tcatacctaa
     6481 cattaccata gaaaggataa gaggtaaagt aaccggcagc ataaattgct gagaatctgt
     6541 ttcgctatca acggctgagc cgatagcagc aaaaagagag gcatacaata aatatcctcc
     6601 tatgaaaaag aataaaaaag caacgatcat cacaccataa ttgattgagt tgaccgcttc
     6661 aactatcatg ttaagtttta ttgtatcacc ttcatccatc tgctccgata tatctattaa
     6721 ctgttcttta ttaagcgctt tcccctgtcc gataaacatt tgttctgact ttgtcagttt
     6781 actcatatcc aaaacggtag tctgcacaac ggtaactatt cccaacgtca atattatcca
     6841 aagggcaaac tgtgtgaggc ctaccattgc tacgccaatt attttaccca tcatcagttc
     6901 gaagggtttt accgacgaga tgattacctc gacaatacgg ttagttttct cctccattat
     6961 accgcgcatg acctgggtgc caaaaaggaa gataaaaatg tatatcagga tcccggcaaa
     7021 aatcccaacg cccatttgta actccgtaaa gcttttctcc tcatcgccgc tttcggatat
     7081 tttgatggta ttaacaacga tattggtttt gatagagcga atcacctccg gatcaatacc
     7141 agaggcttca agcttaagac tctcaacctc gtttttcata atgcttttga tgtatgatgt
     7201 tacttcgagc gaagcctgcc ccgaagagta aaatattgcc gtgcttggta cgctgagttc
     7261 ggttttcgga atccaaacca aaccgtaatc gccgcttttc aacagattgg ccttagccac
     7321 ttctacatct atcgaaagct ttgtaaattt catggtttta gtgtcaacaa aacgatcctc
     7381 aaacaatccc gtatcatcca gaatctgtat tcttttttca tcggtattca tagtggcaag
     7441 tataaaagga agaacccaga tgcctgccat aagaatagga ccaagaaaag tcatcacgat
     7501 aaacgacttt ttcttaactc gggtcagata ctcgcgaagt atgataattt tgatcttgcc
     7561 catgccggtt tattttttaa attcatttac tgttgaaatg aaaatatcgt tcatcgaagg
     7621 cagaatctcg gaaaaaccat gcacttcgac atttttaatt gcgtaattca gcaatatatt
     7681 cggtgaatcc tctacaggta ttttgatcct ggctatagtc atgccgtttt gtgatatctc
     7741 ttccaccaac tgataaccgt caggaagcat ctcggcaaag gatatcgccg atgttggaaa
     7801 ggtgatttcg aaaatctttt gtttaaatga tcgccgtatt tcatcaacgt tcccgtcaag
     7861 gattttctcc gactgattta tcagcgctat atagtcgcaa agctcttcca ccgaggccat
     7921 gttatgagtc gaaaagataa tggtagctcc ctcatcccga agtttaagga tttcatcttt
     7981 caacaggttg acgtttatag ggtcaaatcc ggtaaagggt tcgtcgaaaa tcaaaagatc
     8041 gggtttatgt aaaacagtta ccataaactg aaccttttgt tgcatccctt tcgatagctc
     8101 ttccaccttt ttgtcccacc atgttgttat ttcgaacttt ttaaaccata ctttaagcct
     8161 ctctattgcc tcgtgtttgc tcaacccttt tagctgtgca agatatatag cctgttcacc
     8221 gactttcatc tttttgtaca ggccccgctc ttcgggcaga tatccgattt tcatcacctc
     8281 gtcactgccg agagagcgcc cgttaagtaa aatttcacct tcatcgggag cggtaatgcg
     8341 attgatgatc ctgatcagcg tggttttgcc tgccccatta gggccgagca gcccgaagat
     8401 gctacctctc ggaacgctga tgctcacatt atgaagcgcc ctgtggccgg taaactgctt
     8461 gctgacgttg tttaccctaa gtgtctccat gtagagtagg tttttattaa aattccacgc
     8521 aaaaatagaa acttttcgcc ggcacgaaag atgtttccca attcatcacg tgaatcatac
     8581 caaactccac ctccaaacct caaattgcaa tctattttgc aaacaattaa cgggataaat
     8641 gcagaggata actaacattt aaatgcgcct gctcaatatt tatttttttt acagatacaa
     8701 aggccaaaaa aggaaagttt tcctgacatt atgcaaacag caactacggc aacgacgcac
     8761 cccccggcat ctatataatc gcaatccaaa ttgacggaca acaagcaggc agcgtaaagg
     8821 tggagagaag gtgaaaatct atcctttcaa gatgataata tgattttgca ctaacaataa
     8881 aagagttttg aaaaaaaatt ttttattgtt attgtaaagt tgacaaaatt tagtattttt
     8941 gcttaattaa gtttattatt aatacttaaa aataaaatgt tatgaaaaat attgcttctt
     9001 tgcggatgct tctcctaccc atgctattgt ttttcattat tggaagcggg cgctcacagg
     9061 aaacctacac ccgccccatt accaccccct ggcactggtt gtcgttccca aggctgaagc
     9121 gtgaagcaaa taaccctgtt ttggctatac cggttttaga gaatcttgat ccaatacctg
     9181 acaatttata tttttacagc aatgaaaggg ggcacaataa atttataaaa agaactggag
     9241 gtaattggaa cgaaattaaa ttttattata tatacagtac caaagggtac aagttaaata
     9301 tactggatgt gggttcattt acccacacca tgtatggtac cgatctcaat cccaacacac
     9361 cgattcagct ttatgcaatg cagaaaaaca actcggagaa ttggatcggt tacttccttc
     9421 accggccact ggtgccggag gatgcttttc agggagtgtg ggagtacctt actgagatta
     9481 aaacacagta ttggacaatg gccaagataa ttatcaacgg ccgccctact tggttaagac
     9541 cttccaacgt tacgcctata aaatacggcg atgctttagt ggttaaggtg tcggaggatt
     9601 gcacattgta ttggaaccaa acatgtgaac ccgccggaga aatcctgaga gtagtgccgg
     9661 agtacttcga ctacattgaa tatgccgact atcagccatg gtttatccag accgattcac
     9721 tcggcgacgt gaaggaaata gggctgatgg ccggagatgt ttgcattgga gcctcaacgg
     9781 tgaaaacagg cgataccctt gtaggagtga acgcatatac gcaggctgtt ccgccgggaa
     9841 cgccgattac aatagccaca tgggatgggt taaaatctac agccgccata accggaaagt
     9901 actttgtaat caggcccgaa acgggcagaa aggaatatcg cacggtgcat accggagagg
     9961 gacaacccta ttattacatt tcgttcaccg gccaacaaaa tgaacttcaa caaaacagag
    10021 agcaaacggc atttctctcg ggcatcagcc ctaacccctg caaagaggct gcttttatac
    10081 gttttaatgt tttgaaagag gccgatgttg ccattgaaat ttcagatata aacggaagac
    10141 ggatctcaac agttgcaaag ggtgtgtatc cggaaggcag ttttgaggtg gtatggaaaa
    10201 ccacagatgc atatggcaaa ccggtggaaa acggaattta tatcgtatcg ttagcggttg
    10261 acgacagatt tatcgaaaac caaaagttag ttgtaatcag ataaccccaa agccatgaaa
    10321 aaccttatca tcaccgccat actgttggca agcacgattt cacacgcggc tgtggttacg
    10381 gtgaagcagg acggaacggg cgacttcact catattcagc aggcaatcaa tgctgccgct
    10441 gtaaacgata ctgtattggt atggccggga acctactacg aaaacctaag cttttacggt
    10501 aaagacctta cacttgccag cctatacctt actacgggca aaagggaata tatcgcacaa
    10561 accattattg acggcaataa aaacggaact gtgattaccc tgcaggatgg agaaacatta
    10621 gctacaatga tctgtggatt tactattcaa aacggaaaaa aggattttaa ttcttcttta
    10681 tggagaaaaa tgggatgcgg catttgttta aaacaatcac atgctgttat taaaaataat
    10741 gttatcaaac aaaaccaagg atttactggt ggaggattat cagtcttagg aggttttaca
    10801 aaactatatg gaaatattat aaccgacaac cattcaattt ttattggtgg cggaatttat
    10861 tataacaatt caagtgttcc gatttatctt gatacgattg aactaaatag tattttttta
    10921 aactattcta attggggaag cgatataata gttcgatcta taggtcagca gcagataata
    10981 gaaatagata ccatgacagt atttgatccg gattgttatt ttgcgctctc gattgacggt
    11041 tactccaatc tgatcaatga cctgacgcta aacatccggc atcagaaaat cgaaccggtt
    11101 gctgccgatc tctatgtaag cccacagggg aacaataata attcaggtct taatccggaa
    11161 aatccactta aatctataag cttcgctatt accaaaatcc agcccgacag ccttgtgcag
    11221 cgaacaattc atattttgcc cggcacatat tctcctcaaa caacaggtga aaggctacct
    11281 atcgggggaa gatcaaacat caaattgtcg ggggaagata tgtcaaatac cattattgat
    11341 tgtcaatatg agggttgttt ttattctgga aggtatatga gaaatattga attagaaagt
    11401 tttacgatta aaaatggctt tggggcaatt cttacacctc aaggagtatt tgatgctggt
    11461 ctggatatag attttacgaa aaaggtaaaa atatcaaata ttacaattga tagtgttata
    11521 aacgccgaaa gccagtgtat tagttgtcat gaagttgaca gtatagaaat aaaaaatttt
    11581 cattgtagga acagtattgg agttcaagca aattttttct ttcgaggagg ggaaaatttt
    11641 ccaatgtatg ttagaataga aaatgccaaa atacatggca atacctatta cgatgaaggc
    11701 acatttctat ttggcggtta taaaaatctt attgtatcag gcggtgattt aaacaatgag
    11761 atatgtaccg gaaaatttat aaatgtagaa attaccgaca atatctcttg ggatacctgg
    11821 tacgagcaca cttgcggccc cggttgggca acctccgaaa aagtaaagtt ggatattgta
    11881 aatgccacaa taggaggcaa cagggacagg atgaaccttc caggaaccaa cagcaccgta
    11941 aaaagcagcg aagtaaacat ctacaactcg atcttttacg ataacaactc ccccgatata
    12001 tacatctact gggcaaatga aacaaagcct gcggagatca actttcacta tacactcttc
    12061 gagaacggtg cagcgggcat tatagatagc gccggcaaca ccattgtaaa ttggggggag
    12121 ggcatattgg ataaagaccc gcaatggcaa ggctatggag ccaatccata tgcactgaca
    12181 atcaattcgc catgcattaa cgccggcacg ccgatgtacg aacccggcat gtcgcctccc
    12241 tacataaaag aagaaaacga aagatacatc ctctatacgc caaatatgga taccatccac
    12301 cttccggcaa cagacctgat aggcaacccg cgtatcgcat acggcagaat agatatggga
    12361 gcttacgagt ttgccgatat tacaataaac atcccttcgc cggcaaaact gatcggcgga
    12421 gagattaagg tatttccaaa tccattccaa agcagcacaa atcttcagtt tacactgctc
    12481 aaagggggcc aatgcgaagc gttgatctat gacctgaacg gacggaaggt gaaaacactg
    12541 atgaacgcct ttaccccgcc cggaaactac cggatgcgct ggcacggcga cagcgactac
    12601 ggcaacgacg caccccccgg catctatata atcgcaatcc aaattgacgg acaacaagca
    12661 ggcagcgtaa aggtggagag aaggtgaaaa tctatccttt caagatgata atatgatttt
    12721 gcactaacaa taaaagagtt ttgaaaacgt ctgttttttt taattgaaat ttagcagtta
    12781 aatttcaatt aaatgatttg tacccgatat tgttcttaac aaacacaaaa ccggagttgg
    12841 cttcacaaag tcaataattc ggcttagaaa tgttttctat tgcctaactt gggtttatcg
    12901 tgataaagat ttattctggc atttatcttc ctcgtctaat aatgcgtttg gttattcaag
    12961 gtcaacctca atggttgaac cgttaaaagc ggcaaaaacc cctaatccgc cttcgatatt
    13021 tgaataaacc ggcgatggtt cggaaaaggg attctctcca tcgaaattaa aaacagagcg
    13081 atgataatta taataatgaa tgtcggtcag agctaaaaaa agagtaacag gattaggttc
    13141 ttcatctaaa taaatccaat tggttttata aataaaatat tctccatcct tgttaatgtc
    13201 agtaacgaac tcttcaccct tatccatgta tattgaatac agatgaaagg attctggttc
    13261 aggtgcgtag aaatatttat aaacgccaca aattctatag taatgtcctt gtccatttaa
    13321 atcgcgaaaa cgaaagttga tgatttttcc gccataggta tcgctttgta tgcttaaaat
    13381 ctcaatgtca ggcggatttt caccgggcac agtacaactt gcaaatagtt cttccccctc
    13441 tgaggcttta acgtataaat tataagtctc gccttttttg atctcaagct ccgtgctgat
    13501 atactgctta atagaatcat tgtaggtcaa tggatactct atgttcttat tgcgtatgac
    13561 tatttgtgca tcatccacct cattatttga tggcccttgg cataactccg ttgacgaata
    13621 aagaagcaag cttgaagtga gcattacttt aacggtgtcc ataccgggat ataggtatga
    13681 atgaactact aactttttac ggccagggtc aatatcaaca tcggttatca tgcgttcgca
    13741 agccgacaag acgagaatgt ttataattaa aaatactttt actatacttt tcatggtatt
    13801 aaaatttaaa attatatgta accgaaggaa tgatagggaa tatactcacc tgttttagtt
    13861 tgcttgtttc ggtatttgtt tcccaatcat attctctact gataaaatag aagaatgggt
    13921 taagccggtt atatgcgttg taaagtccaa gctcccagat acgttcatgt tttttcattt
    13981 ttttatgaaa ttgaactgca atatctaagc gatgatacgg tttcatccga aagctattaa
    14041 tttcgccata atcataaatc tccgaccaac tgtagtaatg ataaaaaggt gaatctctca
    14101 tactttcaaa tggatcgtgt gattgagaat aataggagcc taatggtagc gttactgcat
    14161 ttccggttcc atatacccaa cttcctgaga gcgtaatacg tttgttcaac tcatggatca
    14221 ctactatacc aatatcatgt cgccggtcgt aacgcgccca gtattttttt ccaaaattga
    14281 tttcatcaaa ctgaagttgt gtccacgaca aagtataacc tatccagccg cttgtccgcc
    14341 cctccttttt atgcaacaac aattctgctc cgtacgactc tcctcttcct gccgtaacat
    14401 tatcttgcca tgtataactc tgaacttccg tagggtcatc aataagtaaa aaacttgagc
    14461 cgggtttata tccaaggatg tttttgcttt ttttaaaata cccctccaac gaaacttgta
    14521 ttcctttttc caccaattct ttagctatac ctgccgatat ctgtgacgaa tgttgcggtg
    14581 cgattctgtc agtggaaggc acccaaaggt cagttggcaa gcctatacct gtattgctta
    14641 aaagatgaat atactgattc ataaccgaat aagccgcttt gaaggagatg ttttttccaa
    14701 ataaataatt gaccgaaagg cgaggttcgg gagaaaagta atggttttta tcagctataa
    14761 aatgagccat tcgaaagcct gcatttactt gaataatatt tttgaaattg aaatgatttt
    14821 caacataaac ggcgctttca aatgaggtat aatctgtttt actcttaaat tcgtggctta
    14881 cattatcgtc aatttctact atcgcgctgg gtaaaaaata gtgatgcgta ctttgcatcc
    14941 ctgtcctcag gctgtattcg ggagagtgat gatattccaa atcgtattta agcccaacat
    15001 cataaattcc actcgtgtat ttaagctcgt atttatcatt cgaacttcga tattcttcat
    15061 aaatgttcag attgtactcg ctatagatga gcgaggtatt actaaaaacc ctgttgttaa
    15121 agagatggtt ccaacgaatc gttccggttt tattttgcca gtaaagggcg cctttatctt
    15181 tactgttgct atatttgctt acaaaataga atttatcacg tccaaagtat ccgcttaaat
    15241 agagtttgtt ttttgggcca aaatcgtagt tgagttttgt gtttagatca taaaaataat
    15301 aaccgcccct ttcgtcttgt ggcatataag gtgcaataag caagtcaata taggttctcc
    15361 tgcccgaaac caaaaatgat gattttcctt taaccaaagg tccttctaat gtcagccgtg
    15421 aggatattaa tccaatccct ccctgcccgc tgattttttc tttgttacca tctttcattg
    15481 tcatttcgat aaccgatgat agcctgccgc catgccgtgc cgaaaagcct cctttagtga
    15541 gttcaatact tttaattgca tcgccgttaa atactgaaaa gaacccaaaa agatgtgaga
    15601 cattatatac aggggcatca tccaagatta tcagattttg atcggggcag ccacctcgca
    15661 cataaagccc gctgctaccc tccgagcctt tttgtacgcc aggcatcagt tgcaaaacct
    15721 tgaaaacatc cttctcgcct aaaagcgccg gaatattttt tatctgaatc acaggaagcg
    15781 atatcatact catctgatcg ctttcggtca atcgtctata cttttcggca gaaacaacta
    15841 cctccgaaag tgtaagattc gcctccaatt ctacatctat ttcaatgtgt ttatttagat
    15901 ttacctgttc ttcaacaagc tgataaccga catacgaata ctgaattctg tatgagccga
    15961 ccggcaggct taaagagtaa aaaccataat tgttggtagt cgttccggca cgctgttcgg
    16021 gaagataaat atttacaccc ggcaataact cctgactgcc cctctcccgt acaaatccac
    16081 tgatcgtgaa acgactttgc gccgccgaaa aaattgagaa tagcaaaaaa aacaaaatca
    16141 aacaaacaga tgtctttctc ataaaaaaaa ctttggcaat taaaccaaaa ttaagagcgc
    16201 ttattttgcc gcttggaaat ttaatttaag acggatcaaa cttttgagat tgcataagtg
    16261 attaagcgct tattatcaat gcattaaaaa ctgtgctgaa caatcaacaa atcgggattg
    16321 gcctattaat gccgaaacct ttgcacgata aagggtttga atttgataat caattggaaa
    16381 tcgggagaaa cagttattaa tcgttactac agtatagctt acgatctttg tgtcgaaaaa
    16441 aacctatagc gggttagtcc tcttcaaaat gctacttgta gggatatgga actgactttc
    16501 ggttgagcgt tttattataa gtaagtaata agaataaaag tggatgctgt tgtgggtaag
    16561 ttgggtaaaa agaagaaatc ttcgcttaag gagtcaacaa cattcggtaa aaagcgggct
    16621 gaaaaagtaa ttagtgtgtt tcatcagcgc ttccgtttaa atttttgtta gtaacatact
    16681 ggcagcaaat gtaataaaaa gattaaaatg tatgttactc atttttagtt tgttttatta
    16741 attttttatt tttctatttg gttttatctt cttctaacca taaaaaagat atttttgctt
    16801 tttttcattt gttattgaac tcacactaaa ttttaatttt taatatttat gaaaaacatt
    16861 cttgttattg gatcagtagg ccagattggg tcggagttga ctcttgaact tcgcaagagg
    16921 tatggaaata acaatgtagt agctggttat cgtaagactc agccgtcggt ggagcttgcc
    16981 gagagcggtc cgcttgaagt tgttgacgct accgatatcg tccgtttggc agaagttgtt
    17041 aagaaatata agattgacac catttacaat cttgcagcgc tgctatcggc agttgctgag
    17101 aagaacccac aggccgcgtg gaatgtaggc gttaatggag cttacaacgt tttggaagtt
    17161 gcccgtgaat ataaatgtgc tgtctttttc ccaagttcga ttggcgcctt tgggttgagc
    17221 accccacttg ataacacgcc tcaggataca attcagcgcc ctggcacaat gtacggcgtt
    17281 acaaaagtgg ctgccgagct gcttagcgat tactacttca aacgatttgg agtagataca
    17341 cgcggagttc gttttccggg aataatctca aatgttacgc ttcccggcgg aggaacgaca
    17401 gactatgccg tggaaatcta ctatgctgca attaaagacg gtaaatatac atgcccgctc
    17461 cgcaaaggta ccttcctcga tatgatgtat atgcccgatg cgttagatgc tgctattgat
    17521 ctgatggaag ctgacccctc taaattaaaa catcggaact gtttcaacat tactgccatg
    17581 agctttgacc ccgatataat ttataactcg ataaaaaaat atatcccaaa ctttaaaatg
    17641 gactatgatg ttgatcctgt gaaacaaggg atagctgaat cgtggccaaa taaaatggat
    17701 gatagcgctg cccgtgaaga gtggggatgg aacccaaaat gggacctcga caaaatgaca
    17761 caggatatgc taaaggtaat taaagaaaaa catgacaaag ggctgatata gtaaatagct
    17821 aataattttt taaaccggaa gttagggcaa gattaatttc cggttttttt ttgcttgtta
    17881 aagagtgttg ttggaaagta ttagcttgca aatagataat cagtttggga taaatccaaa
    17941 ttcaaaatag agccttataa gcattaacca aaaaaaaccg ctaacttttt attgcttttc
    18001 ttcccaatgt tttgagtcgc cccactcgtt gtaagctatt tcgttttttg ctaatttaat
    18061 acgctcttca agacgtgcta agaaatcatc ggggctatcg gtggtcatag gtttgttttc
    18121 ataaagctta tactgatcgc ggtaagagta gggggtaata ttaggcatta tcacgtttgc
    18181 ccctatttct actgcttttt ctctgccggt tttgtctatt gcttgtagcg cagtggctgc
    18241 agcgatattt atatctttca tcaatatgcg tagcagcgct atcattttca atgataattc
    18301 gaatcttttt ccgattggta ataacagatg acgcaggtca taaagcggtg tatcatgatg
    18361 ttcgatatat ggtcccatac ctaccatatc tacatcaata gatttcatcc aaagcaggtc
    18421 gccggcaagg tcgtcgtaag tttgataagg caatccgatc attaccccgg taccaacctg
    18481 ataaccgacg tctttcaaat attcaagaca ttttaggcga ttatcaaaat catgtaatct
    18541 gttatttgga tgcaatttgt aaaaaagatc acggtttgaa gtctcaattc ttaaaagata
    18601 acgaatagct ccaaggtcaa accatttttg ataggtatct ctttcctgtt cgccacacga
    18661 cagggtaatt ctaagttttt gatttgtggc gccgtgaatt ttgctaagca gattgctaat
    18721 cctgtttaca aaacgcttgt tactcaattc gcccgattgt aggacaatag agccatatcc
    18781 attttcgtaa gcaaaaatgg cggcatctac tatttcatca tcactaagat tataacgctc
    18841 tgttttacgg ttagcgctgc gaatcccaca ataataacaa ttttttgagc agatattgga
    18901 aaactcaatc agccctctga aatatacttt atcttgaaca taaaaacgct ttacctccgt
    18961 tgcttttctg aaaagatctt gcatcgcctc tccttctgcc tgcaacagaa ggataatatc
    19021 ttcgcgtgag aggctgctct tatcaagcag attttttaac gttgacatgc ttcatttact
    19081 aattgtggta cattaaacgg atatttctcc ttttatgtgt gggtggctct gataattttt
    19141 caaaacgaaa tcttcaaatt tgaaatcgaa aatattttta atcaacgggt tgatctccat
    19201 tttgggcaat tggaacggat tacgtgtgag ttgtagtttt acctgttcga tatgatttag
    19261 ataaatatgg gcatcgccaa aggtgtgaat aaactctccg ggcttgtaac cggttacctg
    19321 agcaaacatc atcagcaaca aagcataaga agcaatattg aaaggtactc caagaaaaat
    19381 gtcggcgctg cgctgataca tctgacaaga aagtttgttg ttggcaacat aaaaatgaaa
    19441 aaggatatga caaggtggta gcgccatctt ttctatttct ccaacattcc aagcatttac
    19501 aaggtgtcgg cgcgaatggg ggttctcttt gatcgagtgt attacctggc ttatctgatc
    19561 aatatatcgt ccatcggctg ttggccatga tcgccattga aaaccataaa tagggccaag
    19621 gtttccttcg ccgtcagccc actcatccca gatggttaca ttgttgtcgt gcaagtattt
    19681 tatgttggta tcgcccttta aaaaccaaag caactcatgt attatggaac gtagatgtag
    19741 ctttttagtg gttaatagag ggaaaccttc gtcaagatca aaacgcatct ggtaaccaaa
    19801 gatgctcagg gtacccgttc cggtacgatc ttccttttta accccattgt ccaaaacgtg
    19861 ttctaataaa tctaagtatt gtctcatttt gaaatgtaac ttttatggtt aaaacataaa
    19921 tcatttttaa tggaaaatgt tcaacctgct ttattctgta tctcctccat cacccttaaa
    19981 aagttccctc cccatatttt tataatatcc tcttccacat atcctctcct tagcagctcc
    20041 attgttattt ttgggaattc gccgacatca atacaatctg ccaatcctcc accaccgtca
    20101 aaatcacttc ctatgccaac atggtcaatc ccgatggtct tcacaatatg atcaatatga
    20161 tccacataat ccgacacggt agcaagttcg ataggatact taagattcat tgcctgccat
    20221 tcagcgctag ccatagccga ttcttcatcc gacaagtcct tgaaatatct atatttaatt
    20281 aatagctcat tcataacgct atcacgctgt agattgggtg ggaacgtttt tacataatcg
    20341 ctaaggaggc aaacctgtat aacacctctg ttttttgcca atgcttttaa catttcgtca
    20401 gagaaattgc ggggattgtt acaaattgcc cgtgccgatg agtgcgatgc aataacaggc
    20461 gttgtggata gtctgacgac atcaaaaaag gcgctatcgg aaatgtgcga aacgtcaatc
    20521 atcattccga tgcggttcat ctctttaacc actgcctctc caaaagcgct caacccgtta
    20581 aattcgacag tgtcggttga ggaatcgcag atgtcattgt ttttggaatg acaaagagtg
    20641 atgtaacgag cgcctcgatc ataaaaatat ttaatcagcg aaagatcatt acctatagga
    20701 tacccatttt caattcctaa gagaaaagca cgttttccgc tatatttcag tctttcaata
    20761 tctgaggcat taaaggctat ttcagcctta tcattatttc gttttaatac cgaaacgata
    20821 gaatcgaaaa taagatcggc acgctcaaag gctctcgcat tgcctgcggc cgagcgctct
    20881 ccctgaacta taaatactgc aagaaacaat gcatcaagcc cgccctcttc catacgtgga
    20941 atatctacct tactaccttc ttgacgagca tcatggcgtt gcccaaaatc aaaccctgca
    21001 tgaataaaat acaatggcgt atctgtatga gtatcaatag taatggcttt actatgaatc
    21061 cttaatgcct cttccagcaa ttttttatca gcgcttgtag tagtgcagcc aaccaaaagg
    21121 aacgcaaaaa aaaaagagaa atatttcatt taacgttaag cttaaaaaaa cgaaaatgtt
    21181 agtggcttca aaattatgct tttttaagaa aattattcta aaactattaa gtgcttgatc
    21241 gtaagtattt aatacaaaaa caaaatgttt ttttacaatc tcttaactaa ttatctcttt
    21301 ttagccaatt gggaggttgg atagttaaag taaaaagtgt acttttgaat attcaaaaca
    21361 agaattggtc ttattttcta cctacaaaat aaacaataat gtattcggaa tcaacaaata
    21421 ggctattacc agactcaaaa attttttcat ctatcgttaa tttaatgaca gtgcacccag
    21481 cgcttcgaca aacaataaac gactctgtaa gttattccga ttttcttcaa aacttgaaat
    21541 caacggctct ttcattttta tcaatgaatt cggctattgg agcattactt tcggccggtt
    21601 ggccagtaac taaccaacag atttttatca ctgcagcagc ccgtcgcggc cagtatgcaa
    21661 aactttgaaa agtagtcaac gatattggtg tattgcccta ttatattttc tcagtaaaag
    21721 gatttaatga aaataaacat aattttgcca caaatgcccg atcaatgcag aagagtgcaa
    21781 aagaaaagat tataggacgc ctatctgaaa ataagagcgt ttttttagcc caacagcttt
    21841 cggatggtaa aggtagagtt gacaaaatcc gccgccatag cccgattatt aaacagatgg
    21901 gaaaggaggt tatcgttgaa tcgaaatcgg tagccgaatt tattaggcaa atgatgagaa
    21961 tgggcgagga tggacgggga tatgagacac tttatggcta ttcaataggc ataacggaaa
    22021 agagatttcc gctttacaga tgtcccaaat acgattttga aattaccgac atcctgacaa
    22081 atcttaaact tgaaaatata aatcgcacta acagatgata gattttgata attataattt
    22141 taccctaagt cagctttccg acagagcaaa tagaataaag agtattcctt taaacgacag
    22201 tttaaaaatg gaatattacc gaaagatact caaccttgtt gatctgacga cacttgaagg
    22261 cagcgacact cccgaaaaaa ttgaaacctt atgccgttac gctttaaggc ttcaatccga
    22321 taacccaaat aatggcactg ttgcggcagt atgtatctat atgccatttg tcgagcttgc
    22381 cgtcggacta ttacaaaaca gccctcttga agtggctaca gtagcgggag gatttccatc
    22441 agggcagttg cctgttcatc ttaaaaagca ggaaattgag tatgtcgtgc aacagggcgc
    22501 caacgaagtg gatgttgtaa tatcccgaag cctggcatta atggaaaaat ggaatgagct
    22561 tcaggaggaa ataattcaga tgaaagcttc ttgcggagag acaaaaatga aagtaatctt
    22621 agagacgggc gagctgacag aggtttcaaa aattcgaaaa gcctgtgaat gcgccattaa
    22681 tggaggggct gattttttga aaacatctac cggaaagata aaaccggctg ccacacccga
    22741 agcttttttg ataatattgg atacaattaa cgaataccgt caaaaaacag gcaaaatgat
    22801 tggtgttaag ccggcgggcg gaatagccac acccgatgat gcactgcact actatttgat
    22861 ggtgaaagag actttgggta acgaatggct cgataaaaaa tggttcagaa taggagcaag
    22921 cagattagtt ggaaatctgc ttgagaggat gaaataacaa ataaatgagt agccgccaaa
    22981 ttaaaataat tcaattccaa cttttagtat catcgtaaat tgctggagca aattattcgt
    23041 tttcgctttt ttaaaaaaaa attgtccctg taactatttg gcttttgata ccgtcataaa
    23101 gtcgaaatta aaatttataa atcatgaaaa ctaaaattca aattttaacc ggaattattt
    23161 tagcggtatt tttcattttt aaaaatgaag atgttttagc acaaaagatt aaagggaaca
    23221 tggaggtggt tagtcagaag cgtatagtag atgaatttac cggcatcgaa gcaggcagtt
    23281 tctttgatat ctatctgacg atgaaagacg aacaatcggt tttggtggag actgattcaa
    23341 atcttcagga agaagtaaag acattagtta cagacaacat attgaaaatt aaagcatcta
    23401 accttaaaaa cacctctaag gtaaaggttt atattgctgt gcctgaaatc aactatatta
    23461 atttaagtaa cgctgcgagg gtggaaggaa caaataaaat agaggcttca gcattggaaa
    23521 tcattgctac aggagcttca cagatcaaac ttgacatcaa tgtatccgag ttaatcctta
    23581 atgtaagcgg cgcttctgac gtaaagctga ccggtaatgc aggcaatagc gaaataacta
    23641 ccagtggcgc tgcaaatttg gtagctaaag cgctaaaaac aaatacggca aacataaaaa
    23701 caagcggcgc ctctaacgtt attatttttg ttaacgaaaa tgcaaatacc agcgcaagtg
    23761 gcgccagcga cataaaaatt ctcggagaac cgaaaattga aataaaagat aatgatgaag
    23821 gtacatcacg cgccaaaata tttgcagtag aagaggatga agattataca cgaattaccg
    23881 ctgccggaat agacatcgag gtagttgatg gcgattcaac aatggtaaag gttggcaata
    23941 aatctcttgt cgtggataaa aagggaaacg taaaatacac aggcgacaaa aaaagtaaaa
    24001 agaagtttaa tgggcattgg gcaggattgg atataggaat taacggtttt gtagataaag
    24061 actttaatac cgggaaacct aagggctatg aatttcttaa ccttaattat gagaaatcaa
    24121 ttgatttcag tatcaacttt ttcgagcaga attttaattt gataaaaaac aagttcggca
    24181 tagtaacagg agctggtttg cgggttagca atttccgttt cgaagatcaa agattaatat
    24241 tggtgaatga ttctgcccgg atatttggtt tttccgaccc attggtcaaa tggcagaaaa
    24301 gcaaggttgt aaataattat ctgacagttc ccctaatgtt ggaatatcag actaacccta
    24361 actcaaatcg caacagtttc catctttctg ccggtatgat atttggctgg aaataccgca
    24421 cttacaccaa aatgatggaa aaagaaaacg gtaggaatgt tgttaaaaac agagagacat
    24481 ttcacatgag cccttttaga tacgacctta gcgggcgaat tggatggggc gtactcaatt
    24541 tgtatgcaac ttactcactc aacactttat tcaaaaaaga cgaaggccct aaactgtatc
    24601 cctttgctat tgggattacc attacaggat ggtaagtaaa atccggtcat tacatttgaa
    24661 acaaacccga ttggttcatg aatgcggccg tatcatcaat aaaaagtatt ctctaataat
    24721 tagttaaaaa catagttctt ttttaactct tccgactgaa tttttgcgaa tggttaatca
    24781 gggttgataa taagtttttt ttacatttgc cactcactaa aacgataatt aattgttagt
    24841 tctataaaaa acaattaatg tgaatcagtc ggaaaaatcg aaaatcctta gtagttttta
    24901 ttatccagcc ctgattgtag gttttatgtg gctggttaag gtcctggaaa tggcttttgg
    24961 agttcaattg tataggtttg ggattttacc tttgacatgt aaggggattc caggaatact
    25021 aacctcaccg tttatccatt caaaccttgc ccatattact tcaaacagtc ttccgttatt
    25081 ggttttaggt tcgttattgt tttacattta tcgcactatt gcctggcggg tacttatttt
    25141 tatctggatt atcaccggct tttgggtgtg gatattgggt agggactctt ttcatatagg
    25201 cgccagcggt attgtttacg ggcttgcatt ttttttgttc ttcagtggta ttttccgtaa
    25261 agatagccgg ctacttgcta ttacattttt agtggcgttt ttgtatggaa gtatggtttg
    25321 ggggattttc cccgatcttt ttcctaaaaa aaatatctca tgggaatcgc accttatggg
    25381 aatgattgca ggaatcgttc tcgccttttt ttaccggaat gaaggcgggc ctgcccgaaa
    25441 gatttacagt tgggaaatag aagaagagac agattctgac gatgacgacc ctgatgccta
    25501 ttggaaaaaa gaatctgttg aaaaacataa agataagccc gactcaccca caaaaccttt
    25561 acagataaac tacatttata aaaaatcatc aaaagaagaa aataataaca atacataaat
    25621 ttaaactaat taccatgcgc gaagtatata tcgtttccgc tgtacgtact cctatcggga
    25681 gttttggagg tagtttatcc tcgttatcgg caactcaact gggagccata gttattaaag
    25741 cggcaattga aaaagcggga gtcgccccta acgatgttga tgaagtattg atgggaaatg
    25801 ttttgtctgc caatctgggg caggctccgg cacgccaggc ggcattgttt gccggattac
    25861 ctgattcggt acactgtact acattaaaca aagtctgttc gtcagggctt aagtcagtta
    25921 tgtttggcgc acagtcaata ttgcttggcg ataatgatgt tgtggtggca ggcggtatgg
    25981 agagcatgtc tattgttcca cattatataa cggcggtacg cagaggcatg aaattagggc
    26041 atagccaact cgttgacgga atgattaaag atggcctatg ggatgtatat aacgactatc
    26101 acatgggaaa tgcagccgac aatactgcca aagtaaaagg gattacgaga gatgaacagg
    26161 ataggctcgc aatagagtca tacaaaaggg cagcgaaggc tgttgcagaa gggaggttta
    26221 catcggaaat cgtgccggtt gagagccaaa gtaaaaaagg aaccgtttta gtaaaagagg
    26281 atgaggagta tcgaaatgta aattttgata aaatgcttga attaaagcct gttttcaaca
    26341 aagagggcac agttactgcc gctaacgctt ccaccatcaa cgatggcgct gcagcgcttg
    26401 ttttgatgag taaagaaaaa gccgttgcac tcggaataaa accgttagca aaaattatcg
    26461 gctattccga tgccgcaaat gcccctgagt ggtttactac tgctcccgct aaagcaattc
    26521 ctaaagcagt tgccaaatct ggactaaacc ttgaacagat tgactttttc gaaatcaacg
    26581 aggcctttgc tgttgtgtca ttagctgtga tgaaggagct aaatcttgat cgtgaaaaag
    26641 taaacatttg gggaagcggc atttctcttg gccacccatt gggcgctact ggagcaagaa
    26701 ttttagtaac cttagtaaat atccttcagc aaaacaatgc ccgttatgga gttgcaggcc
    26761 tgtgtaacgg aggcggtgga gcctcagcag ttgtaattga aaatttgaga atttcaaaat
    26821 aaagtaatag cataaatatt ttgaaaataa tccttatcca cttttttcat cttaagagat
    26881 taaaaacaaa ttgaacaaga aaaaagtgct tacgatttaa acagaatatg cgctcgcaca
    26941 gcgagaagta gtgagagttg gatcgcttgc aaaatattta tgatgaattt tattaagttc
    27001 aataaaattt tttgttttct ttagctaagt tgctcataaa caggctgtat aggttaatct
    27061 ttacgtcctc agtctatcaa aaaaaaatta aatttcatta caataaatct ttactcattt
    27121 atcaaatttt aagttatatt tgcagcttca ttttgatctt tactagaaaa acttgaatta
    27181 ctaatttaat taatggatat gaaaaagaat tctaaaagct atatcattaa ttgccccatt
    27241 catcagaagc aacgtagttt ggtaatcgaa gcaaagagaa aaataaacca ttcaaaccgc
    27301 cgggtcgaaa aaaccattta tgctgaagat gttcaatttt tggccaaaca actccttgaa
    27361 tgtgatcagt ttgaagcaaa taaggaagat tgcaaacaat gtcatcaatt tgcaacaacc
    27421 caacatcaat cggttgaagg tgtacttaaa gtgataacca taggatagtc cttaatccca
    27481 agctattttt caattctaat tcttgcgtca acagcagtga tcctatctgc attaccgagc
    27541 agagggttga gatcaagttc cattatttcg ggcgccgctg ataccagagc cgagagcctt
    27601 cgaagtattt ctacaaattg attttcgtta atgcctttcc tgccgcgtat cccctgtatg
    27661 attttgtagc tcttcaaacc acggatcatc tgtaaagcct catcggtcga aagaggggct
    27721 aaacctgtgc tgacatcttt taatacttca atgaatatgc ctcccatacc acaaaggata
    27781 agatggccaa attttggttc aaacttggcg cctgcaaaaa gctcaattcc agagatcatt
    27841 ggctgtataa gaatagctgt agtgtcggga attttgatca tcctatcaaa ttcagcttct
    27901 acctgttcgg catcttcgac attcaacaca acaccgttga cgtccgattt atgaaccggc
    27961 ccaactacct tcattacaac aggaaaacca agcttttcag cgccttcgac tgcctcatgt
    28021 ttatttgtaa acaccccttc gccaactcgc ggaattcctg cactatcaag taaaacttgt
    28081 accttctcag gggcaagata gccatcatca ctactattaa taattcttct aattgattga
    28141 tggtcaacat ctggcaaatc aacatgaaca ggcgctggag caggattgtg atataccctg
    28201 cacagagctt ctcctaacag cgcttcgtca ggaaaattga cacgaccgaa agatagaaac
    28261 tcggcaactt catcactcgc agtaagtact gatggcaaaa caggaaaaat aggcttgcgg
    28321 caactcttca tcttttcgtt caatacgcga tatgcatcga atacttttat caaacctgga
    28381 gttccaaaaa taactaccat cgcatcaatt tcatcaaacg ttttatcgca ataatcaatg
    28441 atgagcccga attgctccac tgtaccggta gcaaggaagt caattggatt agctactgac
    28501 gatccataat acagtttgtc aagtagctct ttagtagccg gatggttgat atgcggcacc
    28561 ttcattcctc catttgaaag cacgtcggta agcataacag cagggcctcc ggcatgagtg
    28621 ataattccaa tattcggccc cggcagttgc ttattcataa aaacagaggc tgtataggtc
    28681 aggtcttcgc gcccatagca gcgtaatatt cctgccttct taaatagagc atccacagca
    28741 acatcggaac tggccagcgc tcccgtgtga gaagaggcag cgcggcttcc tgcttctgac
    28801 gaaccagctt ttatagcagc aatcctacat cctttacgaa ttaaagatga agcatgcttg
    28861 agtagcttct gcggtttagt gattgtttcg atatataaaa gcttcacctt tgaacttgtt
    28921 tctgaatcga aagagacatc catatattca aggatctctt cgacacccat ctgcgcacta
    28981 ttacctaccg agaagacatt gttaaaagtt aaccctttag gaattgccga ctccatgata
    29041 aatacggctg tagagcctga tcctgaaatg aaatcaacac ctttagggtc aggcttagga
    29101 atgggcagag tgaagatact ggcatgtgcc ggagtgatca ctccaataca attaggaccg
    29161 atcagcgaac cttgtaccga gttgacaact tcaacgattt tttcttccaa tcgtctccca
    29221 gcatcactct cttcgccaaa gcctgcagag ataataataa atgcttttgt gcctttatta
    29281 tatgctaaag cctccactac cgagagggta tatttagcgg caatggcaat aatagcaaga
    29341 tcaacctgag gaagatcatc agtattttga aatgaacgga taccctgaac ttctgtctct
    29401 ttgggatttg aaacgtagag cttaccttta aaaccattgt ctataagatt tttcaggatt
    29461 ttacctcctg gctttgaagt atcgtttgag cctccaacaa cgacaatact tttaggattg
    29521 atcaattctt tattaaccat aaatttagaa agattaggtt taaaaatgtt gcgaaaatac
    29581 tattttgcca taaaaaaaca gggtttacaa tttaaattta aagctaattt tgacttttta
    29641 taataaataa tgccttgaaa cgactttata aattaacaag acaaatattg ccaacattac
    29701 tttttttgct ttcggcaata attattgctc aggaggcaaa tgttgaaaac cgagcaatcc
    29761 aaaattatcg ggtgatgttt tataatctgg agaacctttt tgatatctat gatgatcccg
    29821 acacccatga tgatgaattt actcccacgg gctccagaag atggaccaac aaaaaattct
    29881 atgctaaatt aaataatatc tataaagtgg tgatggctgt aggaggatac gaaccaccta
    29941 cagttgtagg aatgtgcgag gttgaaaatg aatttgtatt gcaacgtctc ctcaacaata
    30001 caccacttaa acaatttggg tataaattta ttcattacga ttcgcccgat agccgaggta
    30061 ttgacgtcgc aatgatttac agagatgata aatttacccc tttccattcc gaggccatac
    30121 cggtaatatt ccctgatgat cctgattcga aaacacgaga tatcctttac gtaaagggac
    30181 taattggtgg tagagaaatg attcatattt ttattaatca ttggccgtca agatacggtg
    30241 gatatatgga aactaaacca aaacgcggca gagcagccga gattttaaaa cacaaaaccg
    30301 actccctttt taatattaat ccatcagcag ttattgttat tatgggtgat tttaatgacg
    30361 gcccgaatga tgaaagtcta tttaaaacgc ttgatgccaa aaatcctgaa aatgatatcc
    30421 aaacgacagc gctttacaat atgatgctcg ttgatagaaa aggttgggaa catggaaccc
    30481 tcaaatatcg cgaatattgg gatacatttg atcagataat catctctggc tcattacttg
    30541 acaaaacagg taatttgtgg gtggatccaa atcaagaagt tatatttcat agcgactttt
    30601 tacttcaacc cgatgaaagg tatttgggca aaaaactaat tagaacgtat ttgggattcc
    30661 gatacattgg cggttatagt gatcatttac ccgttttttt tgatttaaag atgaaaatgg
    30721 aataaaatag atattaaaaa aatattacgc tctgttaatc aatgttttaa aatttcaaat
    30781 ttcaaaattt taaattggtt aataaaattt aaatattttt agcggcaaat tccattgtct
    30841 atttggaagc tatttagtga ataaaaacat gtttgagaat aaggataatt tgaatagttc
    30901 agaatcagaa tcggatggca attctcttgc tggccacatg tcagcattct ttaatagaaa
    30961 ccatgaaaat aacctctctt taactaacct gcctgttccc ttacttatag ttgacgaatc
    31021 aggaaccgtt ttgaactgta atcaggaagc tttagagttt ttaaattgta gcgaagatga
    31081 attgaatttt tcactttttg aaatgatacc tcctgattat cagccttata ctaccacact
    31141 attagatagt gcaaaaacca cgggcaagcc gcaaatgggc gaaatgattc tagttctgaa
    31201 ggatggcaaa gagacaaggg taaaaatttt tataaatctt tacgttagaa aaaaagagga
    31261 tgtccttttt accatcgtaa ttgccgacct atctgatgta aaacagtttt atgaagagaa
    31321 gctttttgag atagaattga aatatcgtga attggccgaa agtataaacg aaggtatcta
    31381 catgactgag tggagcacta tcaccaaggt taatacacct ttacttaaga tatttggtta
    31441 tagcgagtcg gaagttatcg gacgtagagt atgggaattt gttgtccctg aatatcgtga
    31501 aaagattaaa aagatctctt tacaaaaaat taaacagatg gataactcgc ccgttgaggt
    31561 acaggttcta cgaaaaaatg gaactcgttt ttgggcagag gtaaggataa atatttttaa
    31621 agaggagcac aagatatttg gtgttttatc agatattaca aatcgaaaag ctgttgaaaa
    31681 agcattgaag gagagtgagt taaagtatcg ctcggtagtt gataccatga atgagggagt
    31741 tgtaatgaga gatatttcgg ggaaggttgt tacctggaac caagctgcgg agagaattct
    31801 tgatatgacc tcagaagaaa tgcctttttt caacatattg gctccacact ggaaagctgt
    31861 aaaagaagat ggaagcgcct tcgatataaa tgaacatcct gctgtaatat ccctctccac
    31921 aggaaaagcc gtacaaaacg tgataatggg aattcacact aaaacaggaa cttttagatg
    31981 gatcaggact aattcagcgc cggtaatgaa taataataaa ggtactataa aggcagccgt
    32041 ttcttcattt tccgatatta catatctaaa aactaccgaa aacaaacttc gggaaataaa
    32101 ttcaataaaa gataagcttt tcagtataat agcccatgac cttaaaagtc cttacaatgc
    32161 ccagatgggt tttcttgaac tacttatgga tgcagaccaa catcatacgc ctgaacaacg
    32221 aaagcaattt attcaaatgc tttacgaaag taccaggcaa tcgttcgccc tacttgataa
    32281 tcttttagtt tggtcgagaa atcaaacggg taaaacaccc tttcaaccaa aagaatttga
    32341 tatcgttgag atgattgata atgcagtcaa aatttttgaa ttatcggcag cagtgaaaaa
    32401 aattgcgatt aaaaccaaaa tagcacaatc aaatttgata gtacgcgccg actatgagat
    32461 gaccaatacc gtgttacaca atctgctatc caacgctata aaatttaccc catccaaagg
    32521 aacaatctct atttcctgca agccggcaaa aagaaatcaa ttgacggttt cggtaaaaga
    32581 tactggaata ggaatgtcag aagaatttgt tgaaaagcta ttcgtttctg atgatataca
    32641 ctctacacca ggcactgaaa atgagaaagg cacggggctg ggactattca tctgtaaaga
    32701 atttgttgaa cgtaacggcg gtagaatttg ggtaaaaagc gctttaggaa aaggaacaac
    32761 attttttttt actataggca gcgctggtaa acacgagcct ggtcaatgta aatgtatatt
    32821 aaacatcgag gatgtttata atgaaattat taaaaacccg gaagtaacag cacgatttta
    32881 taatgatgtt gcctttgctt tttatcagag ctttaaaact tttaaaaata aagattttca
    32941 ggtgttttca aataagttga acaagctgat agatcaatat cagcttgaat cgcttagcca
    33001 gttcgccaaa tgctttaacc aaaattactc tacttgcgaa aaaaaccagt taaacatttg
    33061 ctttcgcgag ctcgaaaagc tatttgataa attgaatatt atgccaatca ttaatccaag
    33121 ataaatcaac actctaagta taatacacat ttttttaccc taagtcagct atagagtaac
    33181 cacaagcgct cctgactggg atacatcaaa aaaaggaact tgattatcag tgcagtacct
    33241 atgccattga cgggctctcc atggtgtatt cgatgaatca aaaataactc tttcaaacgt
    33301 gaacatcttc ctaatttgat ctaaagggat agggcagttt ttctgaagaa tgacccaatc
    33361 tacttgaagc ggtttgtcag aaaaaaatgg ttgaaatttg tcgtttaaaa ttgctattac
    33421 tttatcaaaa gcctgaatga caccatttgc ataaaccttt aaattcccgt ctatttgtgt
    33481 atcctcaact gacattacct cttcgaaaat caattgtttt tgtttgtgta ttccagagta
    33541 tatccttgca tttttaatat taaaatctac agtcttttga tctaataaca aagagctgtc
    33601 ggcaattgag attaaatcct gccccatcac caggtctata gccgaatatc cctttacgtt
    33661 atatacaata attttgttct gttcaatcac cttcattttc tgaaacgtga tgcttgcagt
    33721 aaatattaat aaaagcaaaa gagttataac tccatatttc ccctcttttg caactaaaaa
    33781 tctaactaag aagatgataa caagataaat aataataact tgtgcaaaat tcaacacaag
    33841 tccttcaact cttgcttgcg gcaacttatt aacccattcc actgccatat tcagaaaata
    33901 tacctcgtag taaaggactt tggcaatcca tgccgaaagc tttgacgaca caatagcaac
    33961 aatcagggta gcgataccgg aatatacaat cagccacaca acgggaatta ccacaatatt
    34021 ggcaagcagg aaatagagtg ggaactgatt gaaataaaat attgctaacg gagaggtgcc
    34081 aatctgagct actatggata tagcagcaag tttccaaaaa taatctaata ctgcattctt
    34141 gaaaatcagt tgcctataaa cgagatcata aagtgaaata atcccgataa ccgctgaata
    34201 ggataattga aatccgactt tagtgatcag gtaggggtcg taagcaagca aaatgaaagc
    34261 tgatgctgca aggatattat atgagttaga ttttttccca ataagctcac gccaggtaaa
    34321 taagctaaac atcacgctgg cacgtaatac tgatggagct aatccggtga taaatgcata
    34381 catccaaatg ctacccaaca gaataaagaa tttgataatc cggccagatt ttgaggttag
    34441 ctttttaagt aaaacactta cgatcatgaa tatgataccc acatgtaatc ccgatacaca
    34501 tagtacatgt aaggctcctg ctccggcata atggtttcga agctcaggct ccatgcgatc
    34561 gtcgtaaccg agcaaaatag ctgcgacaac agcaaattca ttcccatcaa ggccctgctc
    34621 ctcaagagta cgcagcatga tgtcacgagc cttgtaagag agactgaaaa ttggattgac
    34681 ctgtcctttt tttatacgtt gccaatcgtt gctgccaagc catgtttgat ggtatatgcc
    34741 actatgagaa agatagcgct ggtaatcgaa ttgatgtggg ttggagggcg aaggaacatg
    34801 gttgtaaatt gctttggcaa caataatatc tccatattta agagtttgac taacactgtc
    34861 tttctcaaga tagataagga tttttccaat agttggaaat tcgttaatgg aatcttttag
    34921 cataacaacc ttcccgaatg cccttacgct tcttgctctt tctgacggct gctcgctgat
    34981 tctgacaacc atcagttcag agccatcagt aacatggctg aaatggttgg aacggtataa
    35041 gtcggtttgt accaaagtaa gagtataacc tgtacttagt acgaaaacat ttaagaacag
    35101 gccgggcaac caacgtaatg aataagtttt tatatagtga gtgaaaatgg tcagaaaaag
    35161 cagtaagagg gttatgatga ttagccacaa tggctgtata aaatgaggaa ctggtagata
    35221 aatcgctgat aggattccta tcccaaatgg gatgataagc ctgaagaaag gatatgaatt
    35281 ccagaatttc atggcatccg tttggattcg atctcaacgg taaaaataag aaaatatctg
    35341 atagaatatg tagttggggg ctatcctcgt gtaaaaaccc atgttgattt gtccgaaaaa
    35401 ctctgattaa aatgataacc ttctgagtca aagagtttta tctcgtcaac attctcaagc
    35461 ctatttgtaa tgatatacct agccatcatc ccccgcgcct ttttgccgta aaccgtaata
    35521 aatttataag agccaccgga gaaatcctta aattgtggag tgataacctc agcttctaat
    35581 ctttccattt ttatagcacg aaagtattcg tcagaggcta agttgataat acatttcgct
    35641 tttcgttctt ttaattgctc atttatcata tttgtaatag aatcccccca atactggtat
    35701 aggtcatcac ctcttttgtt tttaagttta gcgcccattt caagccgata gggttttatg
    35761 agatctaagg gacgcaacaa accataaagg cctgacaaga tacgtagatg ctcctgggca
    35821 aactccaaat catcatcagt taaagtttca gctttcaggc cgttgtacac ttcgccttta
    35881 aacatcaata ccgcgggcaa agcattttcg atagtgaatg gtaaacccca ttccaggtac
    35941 cggagcgcat ttagatcagc aagtttagga ttgacattca ttaacagttg tatctgcgat
    36001 ggagaaaatt tttttaagat ctctactaat tgggctgctt gctttaaaaa atgaggttga
    36061 gtgaaatcaa gtgatggtat ttttacttgt tcgtttaatg tcttggatgg agataataaa
    36121 atatgcataa tcaacgcatt aagaaaataa tgacttaaat tatttgatta aaacgaaagc
    36181 tatcgttaat caaagggtga gagtgaatta attttgtaag gagagcggcc agggtatcca
    36241 aacgtggcgg gataccaagg attatatggg tttacgcctc tgcttatgcc tacttcggcg
    36301 ccaaaatgaa aattatcgtt aacaagatag ttgataccga aagagacccc ttcgccactt
    36361 aaatctaaag cgtaaggatt aacctgtggc tcattaaaaa gagacacctc tttaaacact
    36421 ttggttgaga tcagcaatct ggggttgatc aaatattcac tttcggcgta cataagcgtt
    36481 tgcgtgatgt tatgatcgaa aatctgcatg cgctcaggga agtgaggtat aaaagggttg
    36541 taccaactta aaaaattaga attttgaatt cgaagaccac tattgatcct ccacttatca
    36601 gaaagtttgt aactgaaatg tggcgaagtg aacaccccaa acatctgccc gccagatgag
    36661 ctaaacccaa aagcgctacc aacttcgagc ctgaaactta attttttatc aaaattaaag
    36721 ggtaataatc cggggcgaaa agaacgaata gtaactgaat cctctgaagt taccgccgga
    36781 aaggaattat gattaggctc ctgagcagcc aaatgaagtc ctaaaaacag gatcagtatg
    36841 aagtacaccc ttttcatagt aaagaaactt aaaattagga ggtttcaaat ttagtcataa
    36901 atattaaacc agggtttaat ttcatttcat gcttaaatgg caggcctatc tgatttgtcc
    36961 aactgaacta accaattgaa tacatgataa ataaaaatta tttaaattaa aaccttacta
    37021 aactcaaatt aatccataga gaactccttt ttcatttttc aaaatttttg taaccacttc
    37081 aatttaatag cgtcaaaacc aataaagctc atagacatat tttagtaacc ttttaaaaaa
    37141 taatattatg acctcagttg aaattttagt tccaatcagc ctttttgcta tgatcgcctt
    37201 gattgtgtat atacaggctc gaaaaaagat tcatctaacc ctaatccaac acgggaaaga
    37261 tgcaaaactc ctcaaaacag aaaaagatgc taacatatcg ctgaaaatcg gcctgctgat
    37321 ggtaggcatc tcgcttggta tccttattgg gaacattctt gtagcaaaga agtatatggc
    37381 agaggaacca gcatattttt ccatgattct gatatgcggc ggagttagcc tgatactata
    37441 ttactttctg attaacagag aagcaaaaaa aggaattaaa aaaaagcatg gt
//
LOCUS       DAOOAI010000007        36640 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_1476_length_36640_cov_5.643269, whole genome shotgun sequence.
ACCESSION   DAOOAI010000007 DAOOAI010000000
VERSION     DAOOAI010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 36640)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36640)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36640
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1476_length_36640_cov_5.643269"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="PK345_01080"
     CDS             <1..491
                     /locus_tag="PK345_01080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="succinate dehydrogenase/fumarate reductase
                     iron-sulfur subunit"
                     /protein_id="HPX33646.1"
                     /translation="KFKDGDKITIEPWRSKAFPVIKDLIVDRSVFDKIMQAGGFISAN
                     SGAAPEANSIPIPKDNADAAFDAAVCIGCGACVAACKNSSAMLFVSAKISQFALLPQG
                     KTEAKERARKMVAKMDEVGFGNCTNTGSCETRCPKGLKLTHIAKMNREFITAKIVAEK
                     ST"
     gene            591..1817
                     /locus_tag="PK345_01085"
     CDS             591..1817
                     /locus_tag="PK345_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HPX33647.1"
                     /translation="MNIELFIAQGILSKDKSNFSFPVIKLSIVSVALGLAIMIIAVAV
                     TSGFKNTIADKVIGFGSHLQITSFNLNRSYETEPVLINQPFYPGLKDSAGIRHIQVFA
                     AKSGLIKTKNQIHGVVLKGIWTDFDWSFFQDKIIEGNRLNIDEKQQTDDVLISKKIMN
                     TLQLNLGDAIRVYFVNPEEMTPRARKFNIAGVYETGLEEFDDLYLIGDIRHVQRLNNW
                     GNDEVSGFEVFVSNFRDIDRLTDMVYHSIGYDLNATSIKDIYPQIFEWLDLQDMNVII
                     ILTLMIVVAAINMISTLLILILEKTNMIGVLKALGANNRSVRKIFLYNAAFIIVRGLF
                     IGNLLGISIALLQKHFHLIKLNQESYYVSSVPIQLDFLPIVLLNVGTLVICLLFMLIP
                     SHVIARISPVKAIRWD"
     gene            complement(1828..2430)
                     /locus_tag="PK345_01090"
     CDS             complement(1828..2430)
                     /locus_tag="PK345_01090"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HPX33648.1"
                     /translation="MSLDILHVDSWVFAWIILPLLIFLSRIADQSIGTLRLIFLSKGY
                     KFYAPVLGFFEVIIWLVAVSQILKRMDNALYYVAYGAGFAAGNYIGIILEEKLSLGTV
                     IVRLVPNIDSTDLINNLRENNFGLTLIDADGSRGAVKIIFLIIPRKQLKELVKIINTF
                     NPNMFYTVEEVKSVKHGIIRRENSLINKKHFLPQIWRKSK"
     gene            complement(2519..3226)
                     /locus_tag="PK345_01095"
     CDS             complement(2519..3226)
                     /locus_tag="PK345_01095"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HPX33649.1"
                     /translation="MESDFIEKINRQFADSEPIEVLKYFLKTYKGKIGFASSMGAEDQ
                     ILTEMIVGIDPSARIFTLDTGRIFPETYDLIEKTNTRYKINIEVYFPERDQVEKMVVE
                     KGINLFYESIENRLLCCQIRKTIPLKRALQGLEVWITGLRRSQSVTRTNMQMLEWDKL
                     NNLLKLNPLVEWDEDKVWNYIRKKNIPYNALHDKGFASIGCQPCTKAIKPSEDVRAGR
                     WWWENPEMKECGLHLKK"
     gene            complement(3343..4230)
                     /gene="atpG"
                     /locus_tag="PK345_01100"
     CDS             complement(3343..4230)
                     /gene="atpG"
                     /locus_tag="PK345_01100"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HPX33650.1"
                     /translation="MASLKEVRTRIESVKSTQQITSAMKMVSASKLRKAQTVIHGMQP
                     YAKKMETLLQRVFQPGENQIISPLNEYREPHKILLIAVSSNRGLCGPFNANIARVVIQ
                     RASEHYESQYQNGNIEIFTIGKKVYEYLVARKIKVARRYDELIDKFDSSTVNNLAATL
                     MTDFNTKKYDRVEVIYHHFKNAAVQVITIFQLLPFENPADDKPVKPQDDFDYIYEPSL
                     DKVLEALIPKWLNLKIYEILADSSASEHGARMIAMHKATDNATELLSELKLSYNKARQ
                     SAITNELIEIIGGAEAFQK"
     gene            complement(4241..5833)
                     /gene="atpA"
                     /locus_tag="PK345_01105"
     CDS             complement(4241..5833)
                     /gene="atpA"
                     /locus_tag="PK345_01105"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HPX33651.1"
                     /translation="MSQIKPAEVTEILRQELAGFKTESELEEIGTVLQIGDGIARVFG
                     LSSVESGELVEFEKSDLMGITLNLEQDNVGIVLLGDSRGINEGNIVKRTRRIASINVG
                     YGMLGRVVNTLGKPIDGKGEIKGEVWEMPLERKAPGVIFRQPVNEPLQTGIKAIDAMI
                     PIGRGQRELIIGDRQTGKTAIAIDTIINQREFYEKGEPVYCIYVATGQKGSTVANIVK
                     TLKDHGAMAYTIIVIATASDPAAMQFYAPFAGAAIGEFFRDTGKPALVIYDDLSKQAV
                     SYREVSLLLRRPPGREAYPGDVFYLHSRLLERAAKIIESDEIAKNMNDLPESIRSIAK
                     GGGSLTALPIIETQAGDVSAYIPTNVISITDGQIFLETSLFNAGVRPAINVGISVSRV
                     GGNAQIRSMKKISGTLKIDQAQFRELEAFAKFSSDLDTATLSVLEKGRRNVEILKQDQ
                     YVPMPVEHQIAIIYCGTNGLLKDIPVIKVKNFEELYINKLKTQHPELLKNLKEGKLLE
                     EDEKIMRSVTVEIVNKINNTTK"
     gene            complement(5866..6417)
                     /gene="atpH"
                     /locus_tag="PK345_01110"
     CDS             complement(5866..6417)
                     /gene="atpH"
                     /locus_tag="PK345_01110"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HPX33652.1"
                     /translation="MNITIIANRYAKALFILAQEKNLIDQVYQDMLIVSDVFSKNKNL
                     QRILSSPIYTPAKKLSLLKALFSKHISSFIFRFIELVLRKDRALIIGYIADSYIEIYK
                     EFMNILTVELETAASIDEMTRKEIIRQLTELTKKTIDLEEKVNPKLIGGFIFRYDDRK
                     YDASLRHKIQLLSKEFEKNPYVK"
     gene            complement(6435..6929)
                     /gene="atpF"
                     /locus_tag="PK345_01115"
     CDS             complement(6435..6929)
                     /gene="atpF"
                     /locus_tag="PK345_01115"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HPX33653.1"
                     /translation="MELVSPGLGLIFWMTLAFGVVLWILAKFAWKPIMKSIHEREKSI
                     DNALEQAEEARQEMRNLQANSEEMIRQTKIEQDEVVKATARIKEKMIQDAKEKASAEA
                     EVIIEKTRKQLELEKQAAMIDLKNQIGQLSIEIAEKLLNRELKDKSAQKDYLDELIKD
                     IKLN"
     gene            complement(7096..7356)
                     /gene="atpE"
                     /locus_tag="PK345_01120"
     CDS             complement(7096..7356)
                     /gene="atpE"
                     /locus_tag="PK345_01120"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018335920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HPX33654.1"
                     /translation="MEFLMILLQAGADLSLFAKVGAGLSATIAAAAAGFSIGRIGTSA
                     IESIARQPEAAGDIRANMIVSAALIEGLAFFAIVVCLLIVFQ"
     gene            complement(7410..8549)
                     /gene="atpB"
                     /locus_tag="PK345_01125"
     CDS             complement(7410..8549)
                     /gene="atpB"
                     /locus_tag="PK345_01125"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HPX33655.1"
                     /translation="MSKKFKRVIIPIGIVLILFLGNVFSADGSKQQTEIEQQEDTSYN
                     KKAFDPSKMIFHHIADAHDWHVMDIGDFHFSIPLPIILWHEGKLHVFMSNKLKHGNAL
                     YKGFGLATEASTSKIVYADIDKFNENHQIIQDLKHAAPLDFSITKNVVAIFVSSAIML
                     WVFISVANAYRKRGMVAPGGLQSFLEPVILFVRDDIAKPSIGEKKYKYFMPFLLTIFF
                     FIFLNNVMGLIPIFPGGANVTGNIAVTLVLAAFTLITIILSANKDYWKHIFNTPGVPW
                     ILKIPIPLMPVIELLGLIIKPIVLMIRLFANITAGHIIGLAFVSLIFIFGNTDTFLGF
                     AISPVSVVFSIFIKFIELLVAFIQAYVFTLLSALYIGLAVEEAHH"
     gene            complement(8588..8824)
                     /gene="atpC"
                     /locus_tag="PK345_01130"
     CDS             complement(8588..8824)
                     /gene="atpC"
                     /locus_tag="PK345_01130"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HPX33656.1"
                     /translation="MTIRVISKTKTIYSETVKIAQFPGVDGLFGIMQNHAPMVYVLKK
                     GNIKVKEMNDTEKIIEIEGGLLEVFNNEIIVLAD"
     gene            complement(8844..10355)
                     /gene="atpD"
                     /locus_tag="PK345_01135"
     CDS             complement(8844..10355)
                     /gene="atpD"
                     /locus_tag="PK345_01135"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007213982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HPX33657.1"
                     /translation="MNKTSEGFISQIIGPVVDVSFEHNQALPNIHDALKVVKEDGSFV
                     MLEVQQDIGENTIRSIAMDSTDGLSRGMKVINIGHPIEMPIGEQIRGRLFNVIGEPID
                     GLRAIEKERGYPIHRDPPKFEQLSTTSEILYTGIKVIDLIEPYPKGGKIGLFGGAGVG
                     KTVIIMELINNIAKVYSGLSVFAGVGERTREGNDLLREMIESKVIRYGEDFLKSMESG
                     GWDLSKVDYDELSKSQATLVFGQMNEPPGARARVALSGLSVAEYFRDGGEKAEGRDIL
                     FFIDNIFRFTQAGSEVSALLGRMPSAVGYQPTLATEMGIMQERITSTKRGSITSVQAV
                     YVPADDLTDPAPATTFAHLDATTVLSRKIAELGIYPAVDPLDSTSRILSPHIIGELHY
                     NTSQRVKNILQRHKELQDIIAILGMDELSEEDKQLVRRARKVQRFLSQPFHVAEQFTG
                     IPGKIVDINDTIKGFNMIIDGEVDEYPEAAFMLVGTIDEAIEKGKQMLDQTKN"
     gene            complement(10770..11657)
                     /locus_tag="PK345_01140"
     CDS             complement(10770..11657)
                     /locus_tag="PK345_01140"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPX33658.1"
                     /translation="MKNQSKAYLYAIAATFLWSTIGTAFKLSLRYIAFADLLLFSTLV
                     STFILFLVMVITGKVKLLKDLMFVNWLRGAFLGVLNPFLYYLVLLKAYDLLLAQEAGT
                     LNYIWPVVLVLLSIPLLKQKISWLSVGAILISFFGTLVIGTRGNILTLEFKNPFGVFL
                     AVSSAIFWALFWIFNVKDKNDEVVKLFINFCFGSLFILIYVIASSDFHLPNYQGIVGS
                     VYVGIVEMSITYIIWLKALQLSGTTAKVSNLIYLSPFISLIIIHFIVGEQIFLSTFLG
                     LILIVTGILIQQVLPKIKT"
     gene            complement(11793..15341)
                     /locus_tag="PK345_01145"
     CDS             complement(11793..15341)
                     /locus_tag="PK345_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33659.1"
                     /translation="MKRKSFLQSFVFMIFTILAFSLVSNQQIFSQEIITKWDFDNETL
                     IPSIGEGVADNVGGTSTAFASGLGGGRAWNTAAYPAQGTNEATAGVQLDVSTDGYQNI
                     SVYWDMRHSNTAANRLRLQYTLDGNDWINFEADNSNAVNTQGGNDVGFDNGRYIAESG
                     ELWYVRSADLSSISGATGNALFAIRLVSEFVDGSNYGAANPTSNYSPNGTWRFDNITF
                     SGIPITGGNAVKLVISSVNNGASPTVNIPFEVVVQAQDDDGLPANLNTDTQITLTKET
                     GTGSLEGDLVLTMSAGTYLLTFNNVTYNVVETGVSIKASATSGMTLTSGISAQFSVLE
                     AATQLKFVNVPPNGMINQPIAAFMVEARRPDNSIDPTYNGEITVTKQSGPGNISGTTT
                     KAADAGIATFDDIAFSATGNYTLSANATGLTSDISPEIVIIDAPEIISIIIPQFVSGA
                     DPVDNRLPFAYRVTLKNLFPNSTYRFINQAVITGDSPTTNGAGNVIFVSPDGTFTRTS
                     SPSFTTPNAYGEFTTNQNGSYTGWFMLETTNNARFTPGNHIFMRIRINDGAGGTNVAN
                     YLTVTDSIKVIGFSADTDPNYGTGIRAESMAASKNFAFLYDNATGTGRPLYGTSIETT
                     GIDYNSIPQYTEFYKENVSGVDGAWGGIIPNVNANGVQLIEERNLADGEIAASHVSNN
                     GIWGDANTINPTGGLENILVINLIEDPVVIVNPSSLSGFTYEFGEGPSESQTYKVSGQ
                     NLIGQGFITVTAPANYEISLDGNNFTNTFQLEYLNGQILNQPVTVEVRLKAGLSVGEY
                     NQEQITHTSTITNPVVVTCSGSVTEPIVIPNIATVLLPKYIQGMNGTNVTRLPYAYRV
                     TISNLQPNKTYRYINQVVNYADSPTTNGAGNVIFVKQSESFVRTNNPSMENAGHYGEF
                     TANSSGTYTGWFMTEPTANARFTPGNYLKMRIRINDGMNGTIPIHYLTTADSVQVINF
                     GTTASAEMGTGIRALTNFQPKNFAFIYDNTTGAGQPIYGTSVETTGIDFTQIAQYPEF
                     YKNHVSGINGTWGGIVPNILPNGICRIENRKLSDGTIDSLLISETGIWHNTNTVNPTG
                     GIDDILIIDIIQSVSEPNNENLIKVYSVNDQLMLHVTMDQPINVKIINLLGQTMINKS
                     FSGSYDYQFTHYLKAGAYIVTVSQGTTFFNTKFVVQ"
     gene            complement(15465..16622)
                     /locus_tag="PK345_01150"
     CDS             complement(15465..16622)
                     /locus_tag="PK345_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33660.1"
                     /translation="MTRFFLPLLIFSLTIFSVSLSAQTESNIHLNSPLKTIDTRVITK
                     IPPILKESSGIAVTNPNTIWSHNDANNTNQLFCFDTTGTLLRTLTVINATNVDWEDLT
                     MDNLGRLYIADAGNNDNDRTDLKIYRIPNPDNIAGNIIEAETIKFALEDQYQFPPPAT
                     NKNFDIESILWKNDSIFLFTKNRSSPQNGFCKMYKLPANPGTFIAQLAGSIYLGSVNE
                     EARVCSADINPNTGEVILLTVTKIVSFTKYPNNDFFRGEKNEYYFSSPQGQLEGVQFV
                     DNQRLYITEENSKRSAGNLYEVRWPGTSTVDEYLLKGVNIYPNPVTGNLIIEKEINEP
                     LQVDIWDIKGRMMEVTEKTDSMINLQHLKPGIYFIRLSNNNKLFITRLVKQ"
     gene            complement(16770..17237)
                     /locus_tag="PK345_01155"
     CDS             complement(16770..17237)
                     /locus_tag="PK345_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008767136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HPX33661.1"
                     /translation="MGKEIERKFLVLGNFKHLAFNSFRITQGYLNSLPQRSVRIRVKD
                     NKGFITIKGIGNAQGTTRYEWEREMPLSEAEELLKLCEPGIIDKNRFLIKSGDHIIEV
                     DEFYGANQGLVIAEIELNSEDEEFDKPAWLGKEVTGDQRYYNVYLIKNPYSTW"
     gene            complement(17305..18084)
                     /gene="mazG"
                     /locus_tag="PK345_01160"
     CDS             complement(17305..18084)
                     /gene="mazG"
                     /locus_tag="PK345_01160"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HPX33662.1"
                     /translation="MKEKLAAFERLLNIMDDLRNGCPWDKKQTFESLRYLTIEETYEL
                     SDAILEKDISAIKKELGDLMLHLVFYAKIGSETGDFDIKDVLNDISEKLIQRHPHIFG
                     NVKVTSADDVKNNWEKIKLKEKDRKSVLSGVPNSLPAIVKAFRMQEKVSGVGFDWENA
                     EQVWGKVKEEMEELQNEVRKRDSAKIEMELGDLLFSLVNYSRLIGINPEDALERTNKK
                     FIKRFKYIEEQAKKKGKSLQGMSLPEMDTFWEEAKKTEKQE"
     gene            complement(18155..19114)
                     /locus_tag="PK345_01165"
     CDS             complement(18155..19114)
                     /locus_tag="PK345_01165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HPX33663.1"
                     /translation="MIISLLVLTVGLALLIKGADWLVNGASALAKKYNVSDLVIGLTV
                     VAFGTSAPELVVNVFASAQNHQDIVFGNIIGSNLFNLFAILGISGIIFPLTVQNNTVK
                     KEIPISFLAIIVIFILANDNLFYNSTNVMSRFDGIILLICFCLFLFYIYKQIKNKSDI
                     ADTQVKQLSVLRITLLIVLGLVGLVVGGKLVINSAVDMAKALGVSEKIIGLTIVAAGT
                     SLPELATSVVATVKKNCDIAVGNVIGSNIFNIFFILSVSFIIRPTTYDISFNNDLYVL
                     AGGTLFLFLAMFTGKRKTLDRWEAIILLIAYIIYILLIIYKGY"
     gene            complement(19426..20385)
                     /locus_tag="PK345_01170"
     CDS             complement(19426..20385)
                     /locus_tag="PK345_01170"
                     /inference="COORDINATES: protein motif:HMM:TIGR00367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0098662 - inorganic cation transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HPX33664.1"
                     /translation="MIISLLILTGGLVLLIKGADWLVNGASALAKKYNVSDLVIGLTV
                     VAFGTSAPEMVINVFASAQNHQDIVFGNVIGSNLFNLFAILGISGIIFPLTVQNNTIK
                     KEIPISFLAIIAIFILANDHLFYNSTNVMSRSDGIILFIFFCFFLFYIYKQIKNKSDI
                     TDTQVKQLSVLKITLLIIFGLGGLVVGGKLVINSAVDIAKALGVSKKIIGLTIVAAGT
                     SLPELATSVVATVKKNCDIAVGNVIGSNIFNIFFILSVSFIIRPTTYDISFNNDLYVL
                     AGGTLFLFLAMFTGKRKTLDRWEAIILLIAYIIYILLIIYKGY"
     gene            complement(20514..21785)
                     /locus_tag="PK345_01175"
     CDS             complement(20514..21785)
                     /locus_tag="PK345_01175"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HPX33665.1"
                     /translation="MRVFKFGGASVNNADAVKNVADIITRFSNEELVLVFSAMGKTTN
                     ALEKVTHAAFYNLPELTERIDEVENFHFNIADALFDSDHLQINNKLKNIFDSLKQVAS
                     KPEYAYDEFYDRIVSFGEILSTTIISRFLNERGIMNVIIPAQEMIITGDRFRDAALDW
                     AMTEKLIKKRVLDVFHDQNCNKIITQGFIGSTIDGKPTTLDREGSDFTASVIAYCLDA
                     DEVIVWKDVDGLLNCDPDHFANPLKIDHISYHEVVELTFFGAKILHPKTIKPLQNKSI
                     PLRVKSFFNPTANGTLVSDQASSDLSIPFLIKKNDQALISFFSKDFSFIAEDKLSKIF
                     WRLNQLNIKINLMQNSAISFSIVVNDPGKKLKSLSNSLANDFDILYNRGLELLTIRNF
                     TDQLIKQYLTDKEILLEQRSRKTIQVAYTQT"
     gene            22249..24069
                     /locus_tag="PK345_01180"
     CDS             22249..24069
                     /locus_tag="PK345_01180"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX33666.1"
                     /translation="MKSFIPVEYQKLRKLFSRSDKFKIVAIFVMMLGSIFLEVIGVGI
                     IPAFIAIVANPSMVLENKRFGWIFYQLGITDARSMLIFGAIALIGIFLVKSTYLLLFK
                     FIESRFTYNRRYTLSLRIMTAYMQAPYTFYLTRNSSELLRNTTGEVDNLINQVLNPTL
                     IILKESMLALSLVIFLFIIEPLITFFVILILGGSSLIFLAATQKRVKYFGNQALKYRE
                     EMIKTARQAFGGIKDARVLNREKNFIEAYRVVAYESSLLQRKRAIIVQMPKPIVETFA
                     VIGILLIALVMHIQGRPIADIIPIIALFGAAIVKLMPALFLITQQSTEVRYNIASVNP
                     VHDDLMALQSAQKKFHKDRSQTKIIELQDSIKINNLHYQYPNSTEQALRGVTLEIPKG
                     KAVAFVGPSGAGKTTIADVLLGLLEPQQGKIEVDGKDVFENISAWQYNIGYIPQFIYL
                     SDDTMRRNIGFGLSDKEIDEEKIQKAVDLSQLRELVESLPKGLDAIVGERGTRLSGGQ
                     RQRVGIARALYHNPQVLVMDEATSALDNITEQLIIKAIEDLKGERTVIMIAHRLTTVM
                     NCDIIFYMEEGKIVDQGTYNELIKRNKKFREMALENTKEK"
     gene            24260..25375
                     /locus_tag="PK345_01185"
     CDS             24260..25375
                     /locus_tag="PK345_01185"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPX33667.1"
                     /translation="MGSITHINSYFIASSLHSELVVALDKKGIAQQVFVPVEKACHIN
                     KNEPAKLQFGKIYYSHCFNTLFRYLWPVKMYLIWKAFKKHFLAHPTKLIHAHTLIVNG
                     LIAYFAHRKWNVPYIVSVRHTDMNIFMGNFYFFRIIGYRILKNSKAIIFLSPAYRETQ
                     LKAILGDLAYEKLKQKSYVLPNGINEFWINNRNKKKELTEQHTIIFVGSVVKRKNLKA
                     LINACEILKNEDFKIRLLVVGDGDQLSYFKSQTYKTEIIFYGFISKREQLRNIYKDAD
                     LLVLPSLLETFGLVYPEAMSQGLPVIYTRGQGFDGHYPDGHVGYAINPNDPRDIAEKI
                     KVIFSDYLRFSTNAFNASLDFSWDKIVDKLLEVYSKY"
     gene            25387..26622
                     /locus_tag="PK345_01190"
     CDS             25387..26622
                     /locus_tag="PK345_01190"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPX33668.1"
                     /translation="MKIIFLSNFAGSPYHGMVYGQYYLAREWVKKGHEVCIVSASFAH
                     TRFKQPEQTGKIQEEYIDGIRYIWLPTPEYNPKSSIGRIKSIIIYTLRCRFMKLPVSN
                     ADVVICSSHHPFPIYTAHRLARKFKAKLVFEVRDLWPLTLIELGNVSKQNPFIVLMQC
                     AEDYAYKHAEKVISVLPSAKDYMVGRGMEPEKFLYIPNGADLSTELNAKGLPQNYVET
                     LELIKANGKFIIGYVGKIGLSNALHTLVEALALINDNDIVVVIIGSGAFITDLKILIK
                     KLEVSEKFYFLDQIEKDQVFSFLQYIDVAYVGALKSPLYRFGMSLTKLNDYMLASKPI
                     IYAVEAPNNIIELSGSGISCEAENPEALSQAIQKLKNLSVEDRKKMGTLGREWIIKNH
                     DYKILADRFLDNVIKTNDQ"
     gene            26759..27700
                     /locus_tag="PK345_01195"
     CDS             26759..27700
                     /locus_tag="PK345_01195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009129060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HPX33669.1"
                     /translation="MTKILLTGSSGFVGSNLFDALHQDFELFGLDITKNGKLPENRVY
                     GWDVLSDLPAVDVIIHLAGKAHDTTNTAEEKEYFEVNLNLTQKIFDYFLQSKAKKFIF
                     FSTVKAVADSVEGILNEETTPNPKTAYGKSKLAAEQYINRQPIPDDKLVYILRPCMIH
                     GPGNKGNLNLLYSIVKKGIPWPLGAFQNLRSFTSVENLSYIVVKLIKEQIASGVYQIG
                     DDEPISTNELINLIAKALDRKPKVWSMPKWIILSLTKIGDILKLPLNSERLKKLTESY
                     IVSNQKIKQALQIKNLPISAKDGLLLTLRSFSKDFQK"
     gene            27760..28725
                     /locus_tag="PK345_01200"
     CDS             27760..28725
                     /locus_tag="PK345_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-GlcNAc--UDP-phosphate GlcNAc-1-phosphate
                     transferase"
                     /protein_id="HPX33670.1"
                     /translation="MSFLLYFFISLILIAVILFYFQIATHFDITDKPNVRSSHRNITI
                     RGGGIIFPVAALIWFFLYGFAFKWTIAGLSLIAIISFMDDLKPLSGKVRIIVHFVAIS
                     LMFYDIGIYNLSWYWLLLVYLLTIYWINAFNFMDGINAITPFYSLSALLFFEILNIKL
                     KFFPKELIIILILSVLIFSYFNARKRAKAFAGDVGSVSMAFLLVWMLISLILKTGNIE
                     YILFFSIYILDTLFTLFFRILRRENIFQAHRLHLYQLLSNELKRPHIQVSLLYAISQT
                     IVNLIVVLLIRYELMNFIVFLFILTVLSIVYLAVYRVVTIKIKNI"
     gene            28980..30503
                     /gene="hutH"
                     /locus_tag="PK345_01205"
     CDS             28980..30503
                     /gene="hutH"
                     /locus_tag="PK345_01205"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HPX33671.1"
                     /translation="MAIHKISSALLNFEDILRILRESYQLELSDEAITKIQKCRDYLD
                     RRITDSDDPIYGINTGFGALHNVRISKDDLGKLQQNLVMSHACGTGEEVPQEIVKLML
                     LLKAHALSYGHSGVQVATVQRLIDFFNHDVLPVVYQQGSLGASGDLAPLAHLSLPLIG
                     MGEVYFKGQKMPASYVNNVMGWEPISLQSKEGLALLNGTQFMSAYGIYLCLRVFKLSR
                     LADIIGALSLDAFDGRIEPFHHLIQQIRHHKGQIKTAQRIRNILKDSKLIKRQKAHIQ
                     DPYSFRCIPQVHGASKDSIDYVAYVFRMEINAVTDNPTVFPDEDLIISAGNFHGQPLA
                     LALDNLCIAMAELGNISERRTYQLLEGKRGLPHFLVANSGLNSGFMIPQYTAASIVNQ
                     NKQLCTPASVDTIESSQGQEDHVSMGANAATKAYRVLENLERILAIELLNAAQALDFR
                     RPETTSPYLEEFVAKYREKVQFIENDKVMYNDINTSVKFLQDVVFELPDEDVSVFKL"
     gene            30653..31168
                     /locus_tag="PK345_01210"
     CDS             30653..31168
                     /locus_tag="PK345_01210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPX33672.1"
                     /translation="MTAKEFCKQVLPLQDKIFRLAKKFFNSVDDADDAVQEVFHKLWT
                     RKEDIDSFKSLEAFALIVTRNHCLDTIKAKKHHVTQLNLEHDIADSTSQQKNFENKDE
                     LVTVFKLLNELPELQRTIFHLRDVEGLEFDDIAEILTMNPVTVRVNLSRARKFIREKM
                     IAIQNYEYKRD"
     gene            31149..31682
                     /locus_tag="PK345_01215"
     CDS             31149..31682
                     /locus_tag="PK345_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33673.1"
                     /translation="MNTKEIEILLDKYFEALTTVDEENILRSYFQREDIADHLKSVQS
                     LFWLIERQKAENTETDHGERLMEKISLKKEKGLHQKRLFYFASSIAAIVLLFLSIIYF
                     NTNEKPVPPAKYSPEEIQLAMIQTTNALNYISLKLNKGTSQLYHLQKFEPALHTLAKN
                     LSKLDREIEKVNNQHNP"
     gene            31738..32289
                     /locus_tag="PK345_01220"
     CDS             31738..32289
                     /locus_tag="PK345_01220"
                     /inference="COORDINATES: protein motif:HMM:NF025426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4252 domain-containing protein"
                     /protein_id="HPX33674.1"
                     /translation="MKKIKFVIPFFAFMCLSLLAVGQLPTKSLYQKYSSVKGFSLVTI
                     NKDLFNVLSEINDETKSESGKEINQIINNLDYINILMFDARKSDDPKFLEKFKEEINL
                     LNLANYSELMMVKEEEEEVKIFTLKEKEEIKELVLLISEPQQAGFISIAGNINLKSIG
                     KIARALNIQGLENLEKINTQEEK"
     gene            complement(32491..34653)
                     /locus_tag="PK345_01225"
     CDS             complement(32491..34653)
                     /locus_tag="PK345_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPX33675.1"
                     /translation="MKKIYGFLLTLFLALSFVSYADEGMWLPLFIDRLNYTDMQQKGL
                     QLTAEEIYSINNSSLKDAIIIFGRGCTGEVVSPNGLIFTNHHCGYGQIQAHSSIEHDY
                     LTDGFWAMNLEEELPNPGLTATFLIRIEDVTEKVKVMLNDQMSEKERNSEISKIGKKI
                     TDEATKETHYEASVRSFFAGDEFYLFVYEIFKDVRLVGAPPSSIGKYGADSDNWMWPR
                     HTGDFSIFRIYSGPDGKPADYSKENIPYKPKHFLPISIDGVENEDFTMIMGYPGSTSR
                     YFSSWDVELAIEESNPTVVFIREQKLNIMRKYMDASDAVRIQYASKYAGTSNYWKYCI
                     GQTRGLKRLKVVEKKRLEEADFTSWINGDPARKPIYGEALAMRKEAVGKQREINIAQI
                     YLNEAITRGSEIVLFSRRFNNLLKIINEKTPDNTALDNEIKKLKEISINHFKNYNAKL
                     DEELLGTMLTIYYKNVPQNQQPAFINTLYKKFKGNFSAYANYVFSKSVFTSQEKVSKL
                     LNKPTAKVLEKDPATLLGNAFLDIQQTIRKRYGEINELSAKGERLYIRGLREMKKESK
                     TFYPDANFTMRLTYGSVGDYYPADAVHYDFYSTLAGVMEKEDPSNWEFYVPEKLKELY
                     NKRDYGPYGKNGEMIVGFISNNDITGGNSGSPVMNARGELIGLAFDGNWEAMSGDIIF
                     EPDLQRTISVDIRYVLFIIDKFAGAKNIIDELTIVKHR"
     gene            complement(34680..35771)
                     /locus_tag="PK345_01230"
     CDS             complement(34680..35771)
                     /locus_tag="PK345_01230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HPX33676.1"
                     /translation="MNKDNYCVIMAGGIGARFWPMSRTTRPKQFIDILGNGKTLIQST
                     FKRLERICPKENIFIVTNEIYENQVKHQLPQMASNNILLEPAMRNTAPCIAYANYKIA
                     AINPNANIVVAPSDHIIIDEEEFTNNINSALTAASQNEWLITLGIIPTRPDTGYGYIQ
                     YNDAIRDPNNQNMRKVKTFTEKPNLELAEKFLASGDFLWNSGIFIWSLKTITKAFETY
                     LDDINDLFKEGIGLYNTEKEKSFINDIYTSCQKISIDYGIMEKATNVYVLSADFKWSD
                     LGTWGSLYDTRKKDENNNAVVGNDVLLYDTTNCIINMPAGKLVVLQGLDDYIIVEDSN
                     ALLICRKSDEQEIRRFVSDIKNKKGENFI"
     gene            complement(35865..36473)
                     /locus_tag="PK345_01235"
     CDS             complement(35865..36473)
                     /locus_tag="PK345_01235"
                     /inference="COORDINATES: protein motif:HMM:NF021759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT-like domain-containing protein"
                     /protein_id="HPX33677.1"
                     /translation="MYKLIETLRNHLPKNAIDLVYDQIVKNNIQLTITPTRKSKAGDY
                     RPAVSGKPARISINGSLNPYGFLLVFLHELAHHITWTKYKRSVAPHGKEWKKELKKLH
                     AALASDEIFPDDILNYLKDENRRIYASTHADIKMARTLKKYDKDLNGISIESIPENTI
                     FKTAEGRRFKKLKKRRKNYFCLSLDNQRLYVFSPLLEVYPLN"
BASE COUNT        11110 a         6618 c         6727 g        12185 t
ORIGIN      
        1 ccaagtttaa agatggtgat aaaataacca tagaaccttg gcgatcaaaa gcatttccag
       61 taattaaaga ccttatcgtt gaccgttcag tttttgataa gataatgcag gcaggtggtt
      121 ttatctctgc taactcaggc gctgcccccg aggctaactc tatcccaata cctaaagata
      181 atgccgatgc tgcattcgat gctgcggtat gtataggatg tggcgcttgt gtagctgctt
      241 gtaaaaattc ctctgcaatg ctctttgttt cagcaaaaat ttcacagttc gccttattgc
      301 cgcaaggtaa aacggaagcc aaagagcgcg cccgtaaaat ggttgccaag atggatgaag
      361 tgggttttgg aaattgtacc aataccggct cttgtgaaac acgttgccca aaaggcctta
      421 aattgacgca tattgcaaaa atgaaccgtg agtttattac agcaaagatc gttgcggaaa
      481 aatcaaccta acaaacgcca cattttttag tttttataat tttcaaaatc tataagtcta
      541 aaaggccaag gggattttta taaatagttt ttgagaaaaa cccaattaac ttgaacattg
      601 aactttttat agcgcaaggc atattatcaa aggataaaag taatttctct tttcctgtaa
      661 taaaactttc tattgtcagt gtagcgcttg ggctagctat tatgattata gcggtagctg
      721 ttacttcggg ttttaaaaat acaatagcag acaaagtcat tggttttggc tcacatttac
      781 aaataaccag tttcaactta aatcgttctt acgaaaccga accagtgtta ataaatcagc
      841 ctttttatcc tggtttaaaa gatagcgcag gtattcgtca tatacaagtt tttgcagcaa
      901 aatcagggtt aataaaaact aaaaatcaaa tacacggtgt tgtattaaag ggtatttgga
      961 cagattttga ctggtcattt tttcaagata aaattatcga aggtaatcgc cttaacattg
     1021 atgaaaagca acaaactgac gatgtgttga tttcaaaaaa gataatgaat actctacagc
     1081 ttaaccttgg agatgctata agagtttatt ttgtcaatcc tgaagaaatg actcctcgtg
     1141 ctcgtaaatt taatattgca ggtgtttacg aaacagggct tgaagaattc gacgatcttt
     1201 atcttatcgg cgatattcgt catgtacaac gtcttaacaa ttggggaaat gacgaagtca
     1261 gcgggtttga agtctttgta agcaattttc gcgatattga ccgcctcacc gatatggttt
     1321 atcattcaat agggtatgat cttaatgcta cctccatcaa ggatatctat cctcaaatat
     1381 ttgaatggct cgacctacag gatatgaatg taataatcat cctaacgctt atgatagttg
     1441 tggcagctat caatatgata tctacattgc ttattcttat tttggaaaaa accaacatga
     1501 ttggagtgct aaaagcttta ggcgcaaaca accggtcggt aagaaaaata tttctttaca
     1561 atgcggcttt catcattgtt cgtgggcttt ttatcggcaa tttgttgggt atttccatcg
     1621 cactattaca aaaacatttt cacttgataa agttaaatca ggaatcgtat tatgtatctt
     1681 cagtgccaat tcagttggat tttcttccta ttgtactgct caatgttggc acgttagtta
     1741 tctgtctttt atttatgctg ataccttcgc atgttattgc ccgcatcagc ccagtgaaag
     1801 caataaggtg ggattaacca atttgttcta tttgcttttt ctccaaattt gcggcaaaaa
     1861 gtgttttttg ttgattaatg aattttcgcg cctgattatc ccatgtttta cagattttac
     1921 ctcttccact gtataaaaca tattgggatt aaatgtatta atgattttta ccagctcttt
     1981 tagttgtttt cggggaatga tgagaaatat tattttcact gctcctctac tcccatcagc
     2041 atcaataagg gttaaaccaa aattattttc tctcagattg ttaataagat cggtactatc
     2101 aatattaggt acaaggcgaa caattacggt tcctaacgaa agtttttctt caagtattat
     2161 tccaatataa tttccagcag cgaaaccggc gccgtaagca acgtaataaa gagcattgtc
     2221 cattcgcttt aatatttgac ttactgcaac caaccaaatg atcacctcaa aaaagccaag
     2281 tactggcgca taaaatttgt accctttcga gagaaaaatt aatcttaacg tgccaatact
     2341 ctgatcagca atacgtgaga gaaaaattag tagcggtaaa atgatccagg caaaaaccca
     2401 tgaatcaaca tgtaaaatat ctaagctcat actttcagtc aattttaatg tggttaataa
     2461 aatatttgaa gctgaatgct ttgtaaaaag acattgtaat tattaattga ttttgttttt
     2521 attttttcaa atgtaacccg cactctttca tctctggatt ttcccaccac caccttcctg
     2581 cacgtacatc ttcgcttggt ttaatagctt tggtacatgg ctggcaacca atactggcaa
     2641 atcctttatc atgaagggcg ttgtatggta tattcttctt acgtatataa ttccaaacct
     2701 tatcttcatc ccattcaact aatgggttga gttttaataa attatttaat ttatcccatt
     2761 ctaacatctg catgttagta cgtgttacgg attgacttcg tcttaggccg gtgatccaga
     2821 cttctaagcc ttgcaatgct cttttgaggg gaatggtttt tcgtatttga caacaaagga
     2881 gacgattctc aatgctttca taaaatagat taatcccctt ttcgacaacc attttttcaa
     2941 cctgatcccg ttcaggaaaa tagacctcta tattaatttt ataacgtgtg ttggtttttt
     3001 cgataagatc gtaagtttcg gggaatatcc ggccagtatc taatgtgaaa atacgtgctg
     3061 aaggatcaat accaacgatc atctctgtta atatctgatc ctcagccccc atgcttgaag
     3121 caaatccgat ttttccttta taagtcttca agaagtattt caaaacttct atcggttcgg
     3181 aatctgcaaa ttgccggttt attttttcaa taaaatcact ttccatattc gttctcaggt
     3241 tttaagttta aagatatccg caaatttatt gcaataattt gaaccatctc tccatctctt
     3301 aaaagagata tagctaaaaa tagtcaaaag agtttgagaa tattattttt gaaatgcttc
     3361 agctcctcca atgatttcaa taagttcatt ggtaatagcc gattgtcgag ctttgttgta
     3421 ggagagttta agttcgctga gcagttcagt ggcattatcg gtagccttgt gcattgcaat
     3481 cattcgggcg ccatgctcag aggctgacga gtcggcaagt atttcataaa tttttagatt
     3541 taaccattta gggatcagcg cttcgagtac cttatcgagg gaaggctcat aaatgtaatc
     3601 aaaatcatcc tgaggtttca caggtttatc atcagcagga ttttcaaacg gtaaaagctg
     3661 aaaaatagtt atcacctgta cggcggcatt tttaaaatga tgatagatca cttcaacacg
     3721 atcgtacttt tttgtattaa agtccgtcat caatgttgcg gctaaattat taacagttga
     3781 agaatcaaat ttatcaataa gttcgtcata gcgtctggca actttaatct ttctggcaac
     3841 gagatattca tatacctttt tgccgatggt aaatatttca atattaccat tttgatattg
     3901 actttcgtaa tgttcgcttg cccgttgtat aacaactcta gcaatattag cattaaaagg
     3961 cccgcacagt cctctgtttg atgatacagc aatcagtaat attttatgtg gctccctgta
     4021 ctcgtttaat ggagatatta tctggttctc tcccggctga aacactcttt gtaaaagggt
     4081 ttccattttt tttgcataag gctgcatacc atgaatgacg gtttgtgctt ttcgcaattt
     4141 ggaagcagaa accattttca tggcgctagt aatctgttgg gtagatttta ccgattctat
     4201 tcttgttctg acttccttaa ggcttgccat aataaactaa ttattttgtt gtgttgttaa
     4261 tcttgttgac tatttcgaca gtaacgcttc tcataatttt ttcatcttct tcgagcagtt
     4321 ttccttcctt gaggtttttc aacagctcgg ggtgttgagt ttttaatttg tttatatata
     4381 gttcttcaaa attctttact ttaattacgg gtatatcctt caatagtcca ttagtcccgc
     4441 aataaataat tgcaatctga tgttcaacgg gcatgggtac atattgatcc tgttttaaaa
     4501 tctccacgtt acgccttcct ttttccaaga cggatagggt tgctgtatca aggtcggagc
     4561 taaattttgc aaaagcttcc aattcccgaa actgagcttg gtcaattttc aaggttccag
     4621 atatcttttt catggacctg atctgagcgt taccaccaac acgggataca gaaatgccga
     4681 cattaatagc aggtctgact ccggcattaa ataagcttgt ttcaagaaat atctggccat
     4741 ccgtgatgga gataacgttg gtcgggatat atgccgatac gtcgccagct tgtgtttcga
     4801 taatgggtaa agctgtaagt gaaccgccgc ctttagctat tgagcgtatg ctttcgggca
     4861 gatcgttcat atttttagca atttcatctg actcgatgat cttagcagca cgttccaaca
     4921 atcgggagtg aagataaaag acatcgcccg gatatgcttc tcttccaggt ggacgacgga
     4981 gtaggaggga aacttcgcga tatgaaacag cctgttttga tagatcatca tagataacaa
     5041 gagcgggttt gcctgtgtct ctgaaaaact caccgatagc agcaccggca aaaggagcat
     5101 aaaactgcat ggctgcagga tcggaagccg ttgctattac aattattgta tatgccatag
     5161 ctccatgatc tttgagagtt tttacaatgt tggccaccgt tgatcctttt tgacctgtag
     5221 ctacatagat acaatacacc ggctcgccct tttcataaaa ctcacgttga ttaataattg
     5281 tatcaatagc tattgccgtt ttaccggtct gtcggtcgcc gatgataagc tcgcgctgac
     5341 cgcgtccgat gggtatcatt gcgtcaatag ctttaatacc cgtttgtaaa ggctcattta
     5401 caggctgtct gaaaattact cctggcgctt tacgttcaag aggcatttcc catacctctc
     5461 cttttatctc acctttacca tcaatcggct ttccaagtgt attaactact ctgccaagca
     5521 tcccgtaacc aacattgata gaggcaatac gtcttgtgcg tttaacaata ttaccctcat
     5581 tgatgccgcg actatcacca agcaatacga tacctacgtt gtcctgctca aggttaaggg
     5641 ttattcccat caaatcactt ttttcaaatt ctaccagttc accggattca acactggaaa
     5701 ggccaaaaac cctagcaatg ccatctccga tttgaagcac tgtgccaatt tcttcaagct
     5761 cagattcagt tttgaaccca gcaagttcct gccgcagaat ctcggtaact tcagccggtt
     5821 ttatttgtga catagtattt taatttatca gataacaatt tttaattatt taacgtatgg
     5881 atttttttca aactccttgc taaggagctg aattttatga cgtaggctgg cgtcatattt
     5941 tcggtcatca tatcgaaaaa taaaaccacc gataagttta ggatttactt tttcttcaag
     6001 atcaattgtc tttttagtaa gttcagtgag ttgtcgaatt atctctttcc ttgtcatttc
     6061 atctatagag gcggcggttt ctaactctac tgtaaggata ttcataaact ctttatatat
     6121 ttctatgtaa ctatcagcga tataaccaat tattaaagct ctgtcctttc ttagtaccag
     6181 ctcgataaac ctaaagataa aactgctgat atgttttgaa aatagagctt ttagaaggct
     6241 taactttttt gctggggtgt aaattggact gctgaggatt cgctgaaggt ttttattttt
     6301 tgaaaaaaca tccgatacaa tcagcatatc ctgatagacc tgatctatca gatttttctc
     6361 ctgagccaag ataaacaagg ccttagcata ccggttagca ataattgtta tgttcacttt
     6421 tagaatgttg ccttttaatt gagtttaata tcttttatca gctcatcaag gtaatctttc
     6481 tgagcgcttt tatcctttaa ttctctattg agtaattttt cagcgatttc gatgctcaac
     6541 tggccaattt gatttttcag atctatcatt gctgcctgtt tttccaactc gagttgtttt
     6601 ctggtttttt caataatcac ttcggcttcg gcagatgctt tttccttagc atcctggatc
     6661 atcttttcct tgatacgtgc tgtagcttta accacctcat cctgttcaat tttagtctgc
     6721 cgaatcatct cttcgctatt ggcttgtaga tttctcatct cttgccttgc ctcctcagct
     6781 tgttcaagag cattgtcaat ggatttttca cgttcgtgaa ttgacttcat aataggtttc
     6841 catgcaaatt ttgccagaat ccacaatacc actccaaagg caagtgtcat ccaaaaaatc
     6901 agacctaaac ctggacttac taattccata acttaatgtt tgatgatatt ttaattacaa
     6961 ttagaacgaa aaaatgatag tatttccata ttcgtaagat aaccgaaagt gttgcagata
     7021 taataagttt ttaagataag caaattgatt tctgctcaac tttgattata gccggcgcta
     7081 attgccggca tactcctatt gaaatacaat caacaaacaa acaacaatgg caaaaaacgc
     7141 aagtccttca ataagagctg cagagacgat catgtttgct ctaatatctc cggctgcttc
     7201 tggttgacgt gcgattgact caattgcact tgtacctatc cgaccaatac taaatccagc
     7261 tgcggcagct gctatggttg cagaaagtcc agcacctact tttgcgaata acgaaagatc
     7321 agcgcctgct tgtaataaaa tcattaaaaa ttccataatt taaacatttt aattgaagat
     7381 tattaattaa tttataaaaa atttctttat caatgatgtg cttcttccac agccaaacca
     7441 atgtaaagcg ccgaaagaag tgtaaataca taagcctgaa taaaggctac taaaagttca
     7501 ataaatttta taaaaattga aaatactacc gataccggtg aaatagcaaa acctaaaaaa
     7561 gtgtcagtat taccaaaaat aaaaatcaga cttacgaatg ccagcccgat aatatgtccg
     7621 gcagtgatat tagcaaaaag acgaatcatc agaacgatcg gtttgatgat aagtcctaac
     7681 agttcgataa caggcattaa tggaataggt atttttagta tccatggtac tccgggggtg
     7741 ttaaatatat gtttccaata gtctttattt gctgataaaa ttatagttat caaagtaaag
     7801 gcagccagaa cgagggttac agcaatattt cctgtaacat tagccccgcc cggaaaaatc
     7861 gggatcaacc ccatgacgtt atttaaaaag atgaagaaaa atattgtaag cagaaagggc
     7921 ataaaatatt tgtatttctt ttcgccaata gaaggctttg caatatcatc cctgacaaat
     7981 aaaataacag gttctaaaaa tgattgtaaa cctccaggcg ctaccatgcc gcgtttgcga
     8041 taagcattgg caacagaaat aaagacccaa agcatgattg cagaactaac aaaaatggca
     8101 actacatttt ttgttatgga aaaatccaat ggagcagcat gttttaaatc ctgaataatt
     8161 tgatgatttt cattaaattt atcaatatcg gcatatacta ttttactggt acttgcttcg
     8221 gttgctaatc caaatccctt gtataatgca ttaccatgtt ttaatttatt agacataaaa
     8281 acatgcaatt tcccttcgtg ccaaagaata ataggtaaag gaatggaaaa atggaaatcg
     8341 ccaatatcca taacatgcca atcgtgagca tcagcaatat gatgaaaaat cattttgctc
     8401 ggatcgaatg cctttttatt ataagatgtg tcttcctgct gttcaatctc agtttgctgt
     8461 tttgagccat ctgccgaaaa aacatttcct aaaaacagga taaggacgat ccctataggt
     8521 attattactc ttttaaactt cttactcatg ttttaaaata actgtttatc agccaaggtt
     8581 aattgcatca atccgctaaa acgataattt cattattgaa tacttctaac aaaccccctt
     8641 ctatttcgat gatcttctct gtatcattca tctctttaac cttaatattt ccttttttta
     8701 atacataaac catcggagcg tgattttgca taatcccgaa caggccgtca acaccgggaa
     8761 actgagcaat tttaacggtt tcggaataaa tagttttagt tttgctgatc actctgatag
     8821 tcatattgta cgaatctcct taattaattc tttgtttggt caagcatttg cttccctttt
     8881 tcgattgcct cgtctatagt accaaccagc atgaaagcag cttcggggta ctcatccacc
     8941 tcgccgtcaa taatcatgtt aaatcctttt attgtgtcgt ttatatctac gattttacca
     9001 gggattccgg taaattgttc ggcaacatgg aatggctgtg aaaggaaacg ttgaacttta
     9061 cgtgcacggc gtaccaactg tttatcttct tctgaaagct catccatacc aaggatggca
     9121 ataatatctt gcaactcctt gtgacgttgt aggatatttt ttacgcgctg tgatgtgtta
     9181 taatgaagtt cgcctataat atgaggtgac aatattcttg atgtggagtc aagcgggtca
     9241 acagctggat aaattcctaa ttcggcaatc tttctgctca atactgtagt ggcgtcgagg
     9301 tgggcaaatg tggtggccgg cgctgggtcg gtaaggtcgt cggcaggtac atatactgcc
     9361 tgaaccgagg tgattgatcc tcgttttgta gatgtgattc gttcctgcat tatacccatt
     9421 tcggtggcaa gcgtcggttg ataaccaacg gcggaaggca tacgtcctaa tagggcggat
     9481 acttcggacc cggcttgcgt aaagcggaaa atattatcaa taaaaaacag aatatctctt
     9541 ccttctgctt tttcgccacc atccctaaaa tattcggcga cactaagacc tgaaagagca
     9601 acacgggcac gggcgccagg cggttcgttc atctgcccga acactaacgt cgcctgagat
     9661 ttagaaagtt catcataatc aacctttgac aaatcccaac caccgctttc catactcttc
     9721 aaaaaatcct ctccatacct gataactttt gattcgatca tttcgcgcaa tagatcattc
     9781 ccttcacgtg tacgctcacc aacaccagca aaaactgata aacctgaata tacttttgct
     9841 atgttattga tcaactccat aatgatcaca gtttttccaa caccagctcc accaaacaaa
     9901 ccaattttcc cgccttttgg gtaaggttcg atgaggtcga taactttaat acctgtataa
     9961 agaatttcgg aggtggtgct gagttgttca aacttgggag gatcgcggtg aatcggataa
    10021 cccctctctt tttcaattgc ccgtaagccg tcaattggct cgccaattac attgaaaagc
    10081 cgtccacgaa tttgctcacc gattggcatt tcaataggat gaccaatatt tattactttc
    10141 atccctctgc ttaaaccgtc agtggagtcc atggcaatag accgtatggt attttctccg
    10201 atgtcctgtt gtacttcaag catcacaaaa ctaccatctt ctttaacaac tttaagcgca
    10261 tcatgaatgt tgggcaaagc ctgattatgc tcaaaggaaa catcaaccac cggaccgata
    10321 atctgggaaa tgaaaccctc gctcgtttta ttcatttaaa gtaccttaaa taaaatttat
    10381 ctaaaaattt gtgtgcaaat atgcaattta acttttagct caattgtttt attgccaatt
    10441 tttttaaaag aaaatgtaaa tatcgttcaa ataaatttct ggaaaccaca gaataaaaat
    10501 cttacaatgc caagtactca aagagtttta tgaggatact acctttttaa tcttgaataa
    10561 tttggagaaa gatcttttgg ggcgaattat ttactatacc atatttatat gtctgtaata
    10621 gtccaatttc tattttatct tattgcaata aacagcaatt ttaacggtat taccatccaa
    10681 taattgggat tatgctatca atcccgattt tgtgtttgtt cagatccatt tctattgtgc
    10741 tgaattataa acatcagatc aggatggaat taggtcttta ttttaggtaa aacctgctga
    10801 attaggattc cggttacaat taagatcaat cctaaaaaag tggacagaaa aatttgctct
    10861 cctacaataa agtgtataat aatcaacgag ataaatgggg aaagatagat aaggtttgag
    10921 acttttgcag tggttcccga aagctggagc gctttaagcc agatgatgta agtgatactc
    10981 atctccacaa tcccaacata aacagaaccg actattcctt gataatttgg caagtgaaag
    11041 tccgacgaag cgattacata gattaagata aacagagaac cgaagcagaa attgataaaa
    11101 agctttacta cttcatcatt tttgtctttt acattaaaga tccaaaacag cgcccaaaaa
    11161 atagcgcttg aaaccgcaag aaaaacacca aatggatttt taaactctaa cgttaatatg
    11221 tttccacgag tacctataac taacgtgccg aaaaagctta tcaggatggc gccaacgctt
    11281 aaccagctaa ttttttgctt tagcaatgga attgataata gaacaagaac gacaggccat
    11341 atataattta atgttcctgc ttcttgtgca agcagcaaat cataagcttt aagtaaaacg
    11401 agataataga gaaaaggatt taacactcct aaaaaagccc ctcttaacca atttacaaac
    11461 attagatctt taagtaattt gactttccct gtaattacca ttacaagaaa aaggataaaa
    11521 gtagagacca atgttgagaa taataaaaga tcagcaaagg caatatacct taatgaaagt
    11581 ttaaatgcag tgccaattgt tgaccataga aaggtagccg ctatggcgta aagatatgct
    11641 ttgctttgat ttttcatctt agcaaaagta atttatctat agaaaaactt ttaggctgaa
    11701 ctactgattt tataaagtca atcccgactt attgtttgtt cagaaaattg tttgtactga
    11761 tcggaaatac ttaaattatt gcttgaaaat atctattgaa caacaaattt tgtattaaag
    11821 aaagttgtgc cttgcgaaac agtaacaatg tatgctccag ctttaaggta gtgtgtaaac
    11881 tgataatcat agcttcccga gaagcttttg tttatcattg tttgaccaag taaattgatg
    11941 attttaacat taataggttg atccattgtt acatgcagca tcaattgatc atttactgaa
    12001 taaaccttaa ttaggttttc attatttggt tccgatacac tttgtataat gtcaattatc
    12061 aaaatatcat caattccacc tgtaggatta acagtgtttg tgttgtgcca aatacctgtt
    12121 tctgaaataa gtaatgaatc aatagtgcca tcgcttagtt ttcgattttc gattcgacat
    12181 attccgttag gtagtatgtt tggcactatt ccaccccatg ttccattaat tccagaaaca
    12241 tgatttttgt aaaattccgg atattgcgct atttgagtaa aatctattcc tgttgtttct
    12301 acacttgttc cataaatggg ttgcccagcg cctgttgtat tatcataaat aaaagcaaag
    12361 ttctttggtt gaaagtttgt taaagctctt atccccgttc ccatttctgc tgatgcggtt
    12421 gtgccaaaat ttattacctg cacactgtcg gcggtagtca gataatgaat ggggatagtt
    12481 ccattcatgc catcattgat acggatacgc atttttaggt agttgccggg tgtaaatctt
    12541 gcattcgctg ttggctcggt cataaaccac cctgtatatg ttccgctgga attagcagta
    12601 aattctccat agtgtcctgc attttccata cttggattat ttgtgcgaac aaatgattct
    12661 gattgtttca caaaaatcac attgcccgct ccattggttg tgggagaatc agcatagtta
    12721 acaacctgat ttatgtatct gtaagttttg tttggctgaa ggtttgaaat agtaactcga
    12781 tatgcatatg gcaaccgggt aacattagtt ccattcatcc cctgaatata ttttggtaga
    12841 agaacggtgg caatgttggg tattacaatt ggttcggtaa ctgaaccact acatgttacg
    12901 actaccggat ttgtaatggt ggaggtatgc gttatttgtt cctgattgta ttctccgaca
    12961 ctcaatcctg ctttgagcct aacctcaact gttacaggct gatttaatat ttgaccgttt
    13021 aaatattcaa gttgaaatgt gtttgtaaaa ttatttccat ctaacgatat ttcatagttt
    13081 gctggtgcag taacagttat aaagccctgg ccgataagat tttgcccgga tactttgtag
    13141 gtttgcgatt cggatggacc ctctccaaat tcataagtga atccactaag tgatgaagga
    13201 tttacaataa caacaggatc ttctataagg ttaataacaa ggatgttttc aaggcctcct
    13261 gttgggttta tagtattagc atcaccccag attccgttgt ttgaaacatg acttgcggca
    13321 atctcaccat cagcaaggtt tcgctcttct attagttgaa caccgtttgc attgacattg
    13381 ggtataatgc caccccatgc gccgtcaacg ccagagacgt tttctttgta aaactccgtg
    13441 tattgtggta ttgaattata atctatacct gttgtttcaa tgcttgttcc gtataatggg
    13501 cgtcctgttc cggttgcgtt atcataaaga aaagcaaagt ttttggatgc agccatagat
    13561 tctgctctta tgcctgtgcc atagttggga tcggtatcgg cagaaaagcc tattaccttt
    13621 attgagtcag taactgtaag ataattagct acgttggtac cgccggcgcc atcgtttatt
    13681 ctgatccgca tgaagatatg atttccagga gtaaatctgg cattgttagt tgtctcgagc
    13741 ataaaccagc cagtatagga tccgttttga ttggtggtga actcgccgta agcattaggg
    13801 gttgtaaaac tgggcgagga tgttcgtgta aaagtgccat cgggcgaaac gaaaataaca
    13861 ttgccggcgc cattggttgt tggtgaatcg ccggtaatta cagcctgatt aataaatctg
    13921 tatgtcgaat tagggaaaag gttttttaat gtaacgcggt aggcaaaggg caagcgattg
    13981 tcaacgggat cggcgccact tacaaattga ggaattatta tagaaatgat ctcgggagcg
    14041 tcaataataa caatttcggg actaatatct gatgtgaggc ctgtagcgtt agctgataat
    14101 gtataattac cggtagcgct gaaggcgata tcatcaaaag tagctatacc ggcatcggca
    14161 gcctttgtag tcgttcctga aatattccca ggtccgcttt gttttgtaac tgttatttcg
    14221 ccattgtaag ttgggtcaat tgaattatcc ggtcggcgag cttcaaccat aaaagccgct
    14281 atcggttggt ttatcattcc attaggaggt acgtttacaa atttcaactg ggtggccgct
    14341 tcaagtactg aaaactgagc gctgattcct gatgtcaatg tcattccaga ggttgctgaa
    14401 gcctttatac ttacaccggt ttctacaaca ttgtatgtta cattattaaa agtaagtaaa
    14461 tacgttccgg ctgacatagt gagtacgagg tctccttcta atgagcctgt ccctgtctct
    14521 tttgtaagag ttatttgagt gtcagtgttt aggtttgcag gtaaaccgtc atcatcttgc
    14581 gcctgaacaa caacttcgaa aggaatattt acagtagggg atgcgccgtt attaactgag
    14641 ctaataacta atttaacagc atttccaccg gtgataggga ttcctgaaaa tgtaatattg
    14701 tcaaatcgcc atgttccgtt aggggaataa ttgcttgtgg gatttgctgc tccataattg
    14761 ctgccatcta caaactcgga tactaatctt attgcaaaaa gtgcattacc ggtagcgcct
    14821 gatatactgc taaggtcggc gcttcggaca taccataatt cacctgattc ggctatatag
    14881 cgcccgttat cgaatcccac atcatttcca ccctgcgtat tcactgcatt gctgttgtct
    14941 gcctcgaagt ttatccaatc gttgccatcc aaagtatatt gtaacctgag gcggttggca
    15001 gcggtgttgc tatgacgcat gtcccagtaa actgatatat tctgatatcc atcggtgcta
    15061 acatcaagct gcactcctgc agtagcttca tttgtaccct gagcaggata tgcggcagta
    15121 ttccaagccc taccacctcc taaaccgctt gcaaaggctg tagaagtccc ccctacatta
    15181 tcggcaacac cttcgccaat agagggtatt aaagtttcat tgtcaaaatc ccatttagta
    15241 atgatttcct gactaaatat ttgctgattt gatactaagc taaatgctag tattgtaaaa
    15301 atcataaata caaaagattg taaaaaactc ttccttttca taaaatttaa ttatttgtag
    15361 tgatgtaaat atcttttagt taattttttc ataaggtgaa gaaggtgcaa agtttgttaa
    15421 aataattgtt tcggtataat tgtgttaata acttgatagc taaatcattg tttcactagt
    15481 ctggtaataa ataatttatt gttgtttgat aaacggataa aataaattcc aggttttaaa
    15541 tgttgcaaat ttatcatgct atcggttttt tccgttacct ccatcattct acctttaata
    15601 tcccagatat ccacttgcaa aggttcattt atctcttttt cgatgataag atttccggta
    15661 accggattag gataaatatt gaccccttta agtaaatatt catctacagt gcttgtgcct
    15721 ggccatcgca cctcataaag gtttccggcg cttcttttgc tattctcttc ggtaatatat
    15781 aatcgttggt tgtcaacaaa ttgcactccc tccaactgtc cttgcgggct cgaaaaatag
    15841 tattcgtttt tttctcctct gaaaaaatca ttatttggat attttgtaaa agagacaatc
    15901 tttgtaacag tgagcagaat cacctctcca gtgttgggat tgatatcggc tgagcaaact
    15961 cttgcctctt cgtttacgct cccaaggtaa attgagccgg caagctgcgc tatgaaggtt
    16021 cccggattgg caggcagttt atacatttta caaaaaccat tttgagggct gcttctgttt
    16081 tttgtaaaaa gaaatattga gtcatttttc caaagtatgc tttcgatgtc gaagttcttg
    16141 ttagtagcgg gcggcggaaa ttggtattga tcctcaagtg caaatttgat cgtttcagcc
    16201 tcaataatat ttcctgcaat attatcaggg ttgggaattc ggtatatttt cagatcagta
    16261 cggtcattat cattgtttcc tgcatcggca atgtatagtc tgcctaagtt atccatagtg
    16321 aggtcctccc agtcaacgtt ggtggcattt attacggtaa gggttcgtag tagcgttcct
    16381 gtagtgtcga aacaaaaaag ttgattggta ttatttgcat cattgtgcga ccaaatagta
    16441 ttaggattag ttactgcgat gcccgagctc tctttcagga tgggaggaat tttagtgatt
    16501 actcttgtat caatggtttt aagtgggctg tttaggtgga tattcgattc agtttgagcc
    16561 gaaagagaaa cactaaaaat tgtaagcgaa aagatgagaa gaggtaagaa aaatcgtgtc
    16621 ataaccgaaa gctttttaaa atgtcaaatt ggcttattaa ctccgtgaaa gtaaggatta
    16681 tttttttaaa agatgtttta gaagattttt gtttcacaat actttttatc cctcaatata
    16741 cgtctaacag tgggttttaa tgtagtgttt taccaggtag aataaggatt ttttatcaaa
    16801 taaacgttgt aataacgttg gtcgccggtt acctctttgc cgagccaggc aggtttatca
    16861 aactcttcat cttccgaatt taattctatc tcagcaatta ctaatccttg atttgctcca
    16921 taaaactcat ctacttcaat aatgtgatcg cccgatttga ttaaaaaacg gtttttgtca
    16981 ataatccctg gctcgcatag ttttaatagc tcttcagctt cgctgagagg catttcccgt
    17041 tcccattcat atcgtgttgt gccttgtgca ttgccaatac cttttatagt gataaatccc
    17101 ttattgtctt ttacacgtat tcttaccgaa cgttgtggta atgaattgag ataaccttgc
    17161 gttattctga agctattgaa agctaaatgc ttgaaattgc ctagtaccag aaattttcgt
    17221 tctatctctt tgcccattta tcaggatatt tattttttta ttctaaaatt atgatacttt
    17281 ttttttaatg attgatttac atacctactc ctgtttttca gtcttttttg cctcttccca
    17341 aaaagtatcc atttcaggca agctcatccc ctgaagactt ttaccctttt ttttagcctg
    17401 ctcttcgata tatttaaatc gtttgatgaa ttttttattt gttcgctcta atgcatcttc
    17461 ggggtttata cctattaatc gggaataatt aacaagtgaa aacaacagat cgccaagctc
    17521 catttcaatt tttgcagaat ctctttttct tacctcattt tgaagctctt ccatctcctc
    17581 ttttactttt ccccaaactt gttcagcatt ttcccaatca aatcccactc cgcttacctt
    17641 ttcctgcata cgaaaagctt ttacgatagc ggggagggaa ttaggtacgc cacttaaaac
    17701 cgactttcta tctttctctt ttagtttgat tttttcccag ttgtttttaa catcatcagc
    17761 gcttgttacc ttcacattac caaagatatg cggatggcgc tgaataagct tttcacttat
    17821 gtcgttcaaa acatctttaa tatcaaaatc tcctgtttcg gaacctattt ttgcataaaa
    17881 caccaaatga agcatcaggt cgccaagttc ttttttgatt gcagaaatat ctttttcgag
    17941 tatagcatcc gacagctcgt aggtctcttc tatggtaagg tatctaaggc tttcgaaagt
    18001 ctgcttttta tcccacgggc atccgttgcg caagtcatcc atgatgttga gcaaacgttc
    18061 aaaggcggct aatttctctt tcataaatct cctgtatgtt aagatgatac aaaaatagta
    18121 atttacatct agggaagtgg cagtgtcaac gtggttaata tcccttgtaa atgattaaca
    18181 gaatgtaaat aatataagct attagcagaa taatagcctc ccagcggtca agtgtttttc
    18241 tttttccggt gaacatagct agaaaaagaa ataacgtgcc gccagcaagt acgtataagt
    18301 cattgttaaa ggagatgtca tatgttgtag gtctgattat aaaacttaca gagagaatga
    18361 aaaaaatgtt aaaaatgtta gagcctatga cgttgccaac agctatgtcg cagttttttt
    18421 tcactgtagc cacaacagag gttgccaatt cgggtaatga agtgccggca gcaacaatag
    18481 taagccctat aatcttttca ctaacaccaa gcgcttttgc catatccacc gcactgttaa
    18541 taactaactt gcctcccaca acaagaccta ctaaccctaa aacaatgagc aaagtaattc
    18601 ttaaaaccga tagttgttta acttgagtat cagcaatgtc ggatttgttt tttatctgtt
    18661 tgtaaatgta aaaaagaaaa aggcaaaaac agattaatag aataatgcca tcaaacctgc
    18721 tcattacatt tgtagagtta taaaacagat tatcgtttgc taagataaat attacgatta
    18781 ttgccagaaa agagatgggt atctcttttt ttacggtgtt gttttgaacc gttaatggga
    18841 atattatacc tgagattcca agtatggcaa aaaggttgaa cagattactt ccgataatat
    18901 ttccaaatac gatatcctga tggttttgag ctgatgcaaa aacgtttact accaactctg
    18961 gggccgatgt tccaaaagcg acaacagtta acccgattac caaatcggat acattgtatt
    19021 tttttgcgag cgctgatgct ccatttacaa gccaatcagc gcctttgatt aatagggcaa
    19081 ggccgactgt aagtaccaac aaagaaataa tcatggaaga agttttataa tttgataatt
    19141 ttttaaaaca acgattatta agcgattaat cggcttcatg aaacaatgat tgatctggca
    19201 aaagtataat aacttatgtt aaagctcttc agcaatacga tttaagtttg agtgtaaact
    19261 acctgtatta ttttccgact gtgttgctcc agcagtattt cttcctgact tgtatctttt
    19321 tttattccaa aaaataaacc ataggttttc aattagttat gattatattt tagtgcattt
    19381 gccgcactaa aaggtgcaaa tcaggaaatg tcggtgtcaa tgaggttaat atcccttgta
    19441 aatgattaac agaatgtaaa taatataagc tattagcaga ataatagcct cccagcggtc
    19501 aagtgttttt ctttttccgg tgaacatagc tagaaaaaga aataacgtgc cgccagcaag
    19561 tacgtataag tcattgttaa aggagatgtc atatgttgta ggtctgatta taaaacttac
    19621 agagagaatg aaaaaaatgt taaaaatgtt agagcctatg acgttgccaa cagctatgtc
    19681 gcagtttttt ttcactgtag ccacaacaga ggttgccaat tcgggtaatg aagtgccggc
    19741 agcaacaata gtaagcccta taatcttttt actaacccca agcgcttttg ctatatccac
    19801 cgcactgtta ataactaact tacctcccac aacaagacct cctaacccga aaataatgag
    19861 caaagtaatt tttaataccg atagttgttt aacttgagta tcagtaatat cggatttgtt
    19921 ttttatctgc ttgtaaatgt aaaagagaaa aaagcaaaaa aagataaaca gaataatgcc
    19981 atcagacctg ctcataacat ttgtagagtt ataaaacaga tgatcgtttg ctaagataaa
    20041 tattgcaatt attgccagaa aagagattgg tatctctttt tttatagtat tgttttgaac
    20101 cgttaatggg aatattatac ctgagattcc aagtatggca aaaaggttaa atagattgct
    20161 tccgataaca tttccaaata cgatatcctg atggttttga gctgatgcaa aaacgtttat
    20221 taccatctct ggggccgacg ttccaaaagc gacaactgtt agcccgatta ccaaatcgga
    20281 gacattgtat ttttttgcaa gcgctgatgc tccgtttaca agccaatcag cgcctttgat
    20341 taataagaca aggccgcctg taagtatcaa caaggaaata atcacggaaa aagttttaca
    20401 atttgttgat tttttaaaac agcgtttatt aagcgattaa tcggtttcat aaaacaatga
    20461 ttgatctggc aaaagtataa taacatattt ttaagctctt cggcaatatg attttaagtt
    20521 tgagtgtaag ctacctgtat tgttttccga ctgcgttgtt ccagcagtat ttctttgtct
    20581 gtcagatatt gttttatgag ttgatcagta aagttgcgga tagtgagcag ttccaatcca
    20641 cgattataaa gaatatcaaa atcattagcc agactattgg agagtgattt tagctttttg
    20701 ccggggtcgt ttactacaat tgaaaaactg atagctgagt tttgcattag gttaatcttg
    20761 atattgagtt ggtttaatct ccagaaaatc tttgatagtt tatcttctgc gataaatgaa
    20821 aaatcttttg agaaaaagga tatcaatgct tgatcatttt ttttaatcag aaatggaatt
    20881 gataggtctg aagaggcttg atcactaact aatgttccat tcgctgttgg attaaaaaaa
    20941 gattttaccc tcaacggaat tgatttgttt tgaagtggct taatggtttt tggatgaagg
    21001 attttggcgc caaaaaaggt taactcgaca acttcatgat acgatatgtg gtcaattttg
    21061 agaggattag caaaatgatc gggatcacaa tttagcagac catctacatc tttccaaaca
    21121 attacttcat cggcatcgag gcaataagca ataacggatg cggtaaaatc cgacccttcg
    21181 cgatcaagtg ttgttggttt tccatcaatc gtagaaccaa taaagccttg tgtgattatt
    21241 ttattacaat tttgatcatg gaaaacatct aaaactcttt ttttgataag tttttcagtc
    21301 attgcccaat ccagcgctgc atccctgaag cggtctccgg taataatcat ctcttgcgca
    21361 ggaataatta cattcattat tcccctctcg ttaagaaacc gactgataat tgtcgtcgaa
    21421 aggatttcgc caaaggatac aatgcggtca taaaactcgt cgtaggcgta ttcaggttta
    21481 cttgcaacct gtttaagact atcaaaaata ttttttagct tattatttat ttgtaaatgg
    21541 tcactgtcaa atagggcatc tgcaatattg aaatggaaat tttcaacctc atctattctc
    21601 tccgtcagtt caggtaagtt ataaaaagcg gcatgtgtta ccttttccag cgcattggtg
    21661 gtttttccca ttgccgaaaa aactaaaact aactcttcgt tactgaaccg agtgataata
    21721 tcagctacat tttttactgc atcagcgttg tttaccgaag ctccgccaaa tttaaatact
    21781 ctcatttgat taaataaaag gcaaaaataa atgaatagga taatttcaat tgatttatta
    21841 ctgatttaat cggattaaat ttttatgagc ttgttgatat gaatgactgg taattcattt
    21901 tttacatttt aaaataatta tttttgttga ccttaatgat tgtcgatccg actttatgag
    21961 ggttgttttt tgtttaaaaa taatgaacga ttattaagct gttggttttg ctgggaaatg
    22021 ctgaaataat tgattattat ttactctaaa agtcggatta ttaagatagt tggttaaaat
    22081 gattctgtaa ctgacggggc tgtgctgtaa tatttaaagc taaagatagg taagtccaag
    22141 taaaatcaaa attcaaaaat attagcaata agtgaaattg ataacagttt gaaataaaag
    22201 gcaaatcttt gaatttttaa atttgcacta aaagaaagtg aataaataat gaaaagtttt
    22261 attcctgttg aatatcaaaa gcttagaaag ctgttttcgc gttcagataa gtttaagatt
    22321 gttgcaattt ttgtgatgat gttaggctca attttccttg aagttattgg tgttggtatt
    22381 atacctgctt ttatagcaat agttgccaac ccaagtatgg tattggaaaa taaacgtttt
    22441 ggatggattt tttaccaatt agggattaca gatgcacgtt caatgttgat ttttggtgca
    22501 atagcgctta tcgggatttt cttagtaaaa agcacctatc ttttactttt taaattcatt
    22561 gaatccagat ttacctacaa tcgtagatac actttaagtt taaggattat gacagcctat
    22621 atgcaggctc catacacatt ttatcttacg cgcaatagtt ccgaactttt acggaacaca
    22681 acaggtgagg tagataatct gattaatcag gtgcttaatc ctacgttaat aatattgaaa
    22741 gagtctatgc tggctctttc tttagtaata tttcttttca ttatagagcc attgatcacc
    22801 ttttttgtaa ttcttattct tggaggctcc tctttaattt ttcttgctgc aacacaaaag
    22861 cgtgttaaat attttggaaa tcaagcgtta aagtacaggg aggaaatgat aaaaacagcc
    22921 cgccaggctt ttggtggtat taaagatgct cgtgtgctca atcgtgagaa aaactttatc
    22981 gaagcttacc gggtagtggc atatgaatcc agtttgctgc agcgaaaaag agctattata
    23041 gtacaaatgc ctaaacctat tgtcgaaacg ttcgctgtta taggcatttt gttaatagcg
    23101 ttagtaatgc atattcaggg gaggccaata gcagatatta ttcccattat tgcgctgttt
    23161 ggcgccgcta tagtgaaact aatgcccgca ctttttctta ttacacagca aagcactgaa
    23221 gttcgttata atattgcatc tgtaaatcct gttcatgatg atcttatggc tttacaaagc
    23281 gctcagaaaa agtttcataa agaccgtagc caaacaaaaa tcattgaact gcaagattcc
    23341 ataaaaatta ataatttgca ttatcaatac cctaacagta ccgagcaggc tttacgtgga
    23401 gtaacactcg aaatacctaa agggaaagct gttgcttttg ttggcccttc cggcgctgga
    23461 aaaaccacta ttgctgatgt cttattggga ttattagagc cacagcaagg aaaaattgaa
    23521 gttgatggta aggatgtttt tgaaaacata tcagcctggc aatataatat tggttatatc
    23581 ccacaattca tatatctttc agatgatacg atgcgccgta atattggatt tggactttct
    23641 gataaagaga tagatgaaga aaaaattcaa aaggcagttg atctttcgca gctacgtgag
    23701 cttgttgagt ctttgccaaa aggattggat gctatcgtag gcgaacgagg gacaaggctt
    23761 tcaggaggtc aacgtcagcg tgtaggtatt gcccgtgccc tctatcacaa tccacaggtg
    23821 ttggttatgg atgaagctac ttcagcgctc gataatatca ctgaacaact tatcataaaa
    23881 gctattgaag atctgaaagg tgaaagaact gtaataatga tagcgcacag gctcacaacc
    23941 gttatgaatt gtgatatcat tttctatatg gaagaaggaa aaatagttga tcagggtact
    24001 tacaatgaac tgataaagag aaacaaaaag tttagagaga tggcattgga aaatacaaaa
    24061 gaaaaataat tgacaataag atgctttatc attgtttaca agtctttttg attttttaaa
    24121 aattaaattc aaggtgtgca ttagactttt acagaacggc ataattattt ttagaactaa
    24181 tatggatttg tatctttttt ttaaaagatc ttacgagggt attcaaaaca gtaagcgaat
    24241 aattgtttta tagttatata tgggttccat tactcacatt aattcctatt ttatcgccag
    24301 ctcgcttcac agcgaattag ttgtagcatt ggataagaag gggattgccc aacaggtgtt
    24361 cgttccggtg gagaaagcct gtcatattaa taaaaatgaa ccggctaaac tgcaattcgg
    24421 gaaaatttat tactcacatt gctttaatac tttattcagg tatttatggc cggttaaaat
    24481 gtacctgatt tggaaagctt ttaaaaaaca ttttttagct cacccaacca aactaatcca
    24541 tgctcatact ttaattgtaa atggcttgat agcttatttt gctcaccgaa aatggaatgt
    24601 gccctatatt gttagcgtac gtcatactga tatgaacatc ttcatgggga atttttattt
    24661 ttttagaata attggatatc gtatattgaa aaattcaaaa gctattattt ttctatcacc
    24721 tgcatacaga gaaactcaat tgaaagctat acttggagac ttagcttatg aaaaactcaa
    24781 acaaaaatct tatgttttac caaatggaat taatgaattt tggataaaca accgtaataa
    24841 aaaaaaggaa ctaacggaac agcacaccat catttttgtt ggtagtgttg ttaaaagaaa
    24901 aaatctgaaa gctcttataa atgcttgtga aattcttaaa aatgaggatt ttaagataag
    24961 gttattagtt gttggtgatg gagatcaact ttcatatttt aagagtcaaa cttacaaaac
    25021 ggagataatc ttttatggtt ttatctcaaa acgcgaacaa ttaagaaata tttacaaaga
    25081 tgctgaccta ttggtattgc caagtttgtt ggaaacattt ggccttgttt atccggaagc
    25141 tatgtcgcag ggtttgccgg taatatacac ccgtgggcag gggtttgatg gacattaccc
    25201 tgatggtcat gttggctatg ccatcaatcc taatgatcca agggatattg ctgaaaaaat
    25261 caaggtgatt ttttctgatt acttacgttt ctcaaccaac gcttttaatg catcgcttga
    25321 cttttcctgg gataaaatcg tagataaatt acttgaggtt tattctaaat attaattttt
    25381 taggttttga aaatcatttt tctttccaat tttgccggct caccttatca tggtatggtt
    25441 tacggacagt attaccttgc ccgggaatgg gtgaaaaaag gccacgaagt ttgcattgtg
    25501 tcagcttcat ttgcacacac ccgtttcaaa caaccggaac aaacggggaa aattcaagaa
    25561 gagtatattg atggaattag gtatatttgg ttgccaacac ctgaatataa tccaaaatcg
    25621 tcaataggca ggatcaaaag tattatcatt tatactttac gttgccgttt tatgaaactc
    25681 cctgtttcaa atgctgatgt tgtaatttgc tcatctcatc atccatttcc catttatact
    25741 gcacatagat tagccagaaa attcaaagca aagcttgtgt ttgaagttcg tgatctatgg
    25801 cctttaactc ttatcgaact tggcaatgtg tcaaaacaaa atcctttcat agtacttatg
    25861 cagtgcgctg aagattatgc ttataaacat gctgaaaaag tgatttctgt tttaccgtcc
    25921 gccaaagact atatggtggg caggggcatg gaacctgaaa agtttttgta tatcccaaat
    25981 ggtgcagacc tatcgactga actcaatgca aaaggattac cacaaaatta tgttgaaact
    26041 cttgaattga ttaaagcaaa cggtaagttt attatagggt atgttggaaa aatcggtctt
    26101 tcaaatgctc ttcatacttt ggttgaagca ttagcgctga taaacgacaa tgatatagta
    26161 gttgtaatta tcggttctgg cgcttttatc accgatctta aaatcttaat aaagaaatta
    26221 gaagtttcgg aaaaatttta ttttttagat caaattgaaa aagatcaggt atttagtttt
    26281 ttacaatata ttgatgttgc ttatgttggc gctttaaaaa gcccattata tcgctttgga
    26341 atgagtttga caaaactgaa tgactatatg cttgcaagta agcctattat atatgctgtc
    26401 gaagctccaa ataatattat agaattaagt ggatccggca taagctgtga agctgaaaat
    26461 cctgaagcat taagtcaagc catacagaaa ttaaagaatt tgagtgtcga agatcgaaaa
    26521 aaaatgggaa ctcttgggcg tgaatggatt ataaaaaatc acgattataa gatacttgcc
    26581 gatcgttttt tagataatgt tattaagaca aatgatcaat aatctattga ataaatagtt
    26641 agatcgtgac taaatcccaa taatactttc aaacctccta tttctgtttt gcaaaatgtt
    26701 tcagaaatat ggagttttgg attgaacctg aactaatcag ggttaaaaaa taagcaatat
    26761 gactaaaatc cttcttactg gctcatccgg ctttgttgga tcgaatctgt ttgatgcttt
    26821 acatcaggat tttgaactgt ttggattaga tattacaaag aatggtaaac tacctgagaa
    26881 cagagtgtat ggatgggacg ttcttagcga tcttcctgct gtagatgtta ttatacattt
    26941 ggccggaaag gcccacgaca ctacgaatac ggctgaagaa aaagagtatt tcgaagtaaa
    27001 cctgaatttg acacaaaaaa tctttgatta tttcttgcag tcaaaggcta agaaatttat
    27061 tttttttagt actgtaaaag ctgttgccga cagtgttgag ggcatattaa atgaagaaac
    27121 aacaccaaat ccaaaaactg catacggaaa atcgaaattg gctgccgagc aatatataaa
    27181 ccgacaacca attcctgatg acaagctggt ttatatcctt aggccttgta tgattcacgg
    27241 gcctggtaat aaaggaaact taaatctttt atatagtatc gtaaaaaaag gcattccttg
    27301 gccgctcgga gcctttcaaa atttacgctc gtttacatcg gtcgaaaatt tgagttatat
    27361 agtcgtgaag ttgataaaag agcagatagc ttcgggagta tatcaaattg gcgatgatga
    27421 acctatatca accaatgaat tgatcaatct tatcgcaaaa gcattggata ggaaaccaaa
    27481 agtatggagt atgccaaaat ggataatcct tagtttaact aagatcggtg atattttaaa
    27541 actcccatta aattcggaaa gattgaaaaa actaaccgag tcgtatatag tatcaaatca
    27601 aaagattaaa caagctcttc aaatcaagaa ccttcctatt tctgcaaaag atgggctatt
    27661 gctaacgttg cgaagctttt ctaaagattt tcagaaataa tgttttaaag attctttctt
    27721 gctaaataaa taggaataat caatgtaaat ttcccaattg tgtcttttct actctatttt
    27781 ttcatctcgc tgatacttat tgctgtaatc ttattttatt ttcagattgc aacccatttt
    27841 gatattacgg acaagccaaa tgtgcgtagc tcacaccgca atataactat tcgcggtgga
    27901 ggaattatct ttcctgttgc agctttaatt tggttttttc tctatggatt tgcatttaaa
    27961 tggacaattg caggcttgtc attaattgca ataatcagct ttatggatga tctcaagcct
    28021 ctttcgggta aggtaaggat aattgtacac tttgtagcaa ttagcttaat gttttatgat
    28081 ataggtattt ataatttgtc atggtattgg ctgttgttgg tatatctttt aacaatttac
    28141 tggatcaacg cctttaactt tatggatggt attaatgcaa tcactccatt ttacagccta
    28201 tcggcactct tattttttga aatattaaat atcaagttaa agttttttcc taaagagctt
    28261 attataattc tcatactttc cgtattgatt ttcagttatt tcaatgctcg caagcgcgct
    28321 aaagcttttg ccggagatgt gggtagtgtg agtatggctt ttttacttgt ctggatgtta
    28381 atttcactga tcttaaaaac aggtaacatt gagtacatac tctttttttc aatatacatt
    28441 cttgatactt tatttaccct cttcttccgt atcctacgtc gcgaaaatat ttttcaggca
    28501 catcgcctgc atctttacca gttactctct aatgaattaa aaaggcctca catccaggta
    28561 tctctattat atgctatttc tcagacgatt gttaatctta tagtcgtttt attaatacgg
    28621 tacgaactaa tgaacttcat agtgtttcta ttcatattaa ccgttctatc aattgtttat
    28681 ttagcagtat atcgtgttgt aacaataaag attaagaata tttgagtttt gttatcctta
    28741 attattagaa agtacttttg agctgaacta ttgttttatg aaggtgattt cgacttggta
    28801 tttattcagt agaattctta tgatgttgat tacaaccgct taattcgaaa ttaatgagat
    28861 aaaaagaaag agggctttgg tcctcttttt ttttggaaaa attgtcctta catcttctat
    28921 tgattatcca taagggatat tttattctct atttttgtca aaataaatgt ttgtttgcta
    28981 tggctataca taaaattagt tctgcactat tgaattttga agatatcctt cgtattttgc
    29041 gagaatcata tcagcttgaa ttgtctgatg aagcgataac taaaattcag aaatgccgtg
    29101 attatctcga tcgcaggatt actgacagcg atgatcctat ttatggtatt aatacaggtt
    29161 ttggcgcctt gcataatgtg cgtatttcaa aagatgattt gggtaaattg cagcaaaatc
    29221 tggttatgtc tcatgcttgt ggtacgggag aggaggttcc acaggaaata gtaaagttaa
    29281 tgttgcttct caaggcacat gctctttcct atggtcactc gggagttcag gttgccacag
    29341 ttcagcggtt gattgatttt tttaaccacg atgtgttgcc ggtagtatat cagcaaggtt
    29401 ctcttggcgc ttcaggcgat cttgctcctt tggctcatct cagtctgcca ctgataggaa
    29461 tgggcgaggt ttattttaag ggtcaaaaga tgcccgcttc ctatgtaaac aatgtaatgg
    29521 gatgggaacc tatcagtctg caatcgaaag agggactagc gttgcttaat ggcacgcagt
    29581 ttatgagcgc ttatggtatt tatctctgtt tgcgtgtatt caagctgtcg cgcctggcag
    29641 atattattgg agctttgtct ttagatgcat ttgatggcag aatagaaccc tttcatcatt
    29701 tgattcagca aataaggcat cataaaggtc agataaaaac ggcacagcgc attcgtaata
    29761 ttttgaaaga cagcaaactt attaagcgcc aaaaagctca tatccaagac ccatactcct
    29821 ttcgttgtat acctcaggta catggtgcat ctaaagattc aatagactat gtggcttatg
    29881 ttttccggat ggagattaat gcagttaccg acaaccctac agttttccct gatgaggatc
    29941 taattatatc tgcaggtaat tttcatggtc agccattagc tttagcgctc gataatttgt
    30001 gtattgctat ggctgagctt ggtaatattt ctgagagacg tacttatcaa ttacttgaag
    30061 gcaaacgtgg gctaccacat tttttggttg caaattcggg acttaattca ggctttatga
    30121 tcccacaata cacagcagct tctattgtta accaaaacaa acagttatgt acccctgctt
    30181 cggtggatac tattgagtcg tcgcaaggtc aagaggatca cgtgagtatg ggcgctaatg
    30241 cagctaccaa agcctacaga gtacttgaaa atttagaacg gatacttgct attgaattgc
    30301 taaatgcagc gcaggcgctc gattttagaa gaccagaaac tacatcgcca tatcttgaag
    30361 aatttgttgc gaaatatcgc gaaaaagtac aatttattga aaacgacaaa gttatgtata
    30421 acgatattaa tacttctgtt aagttccttc aggatgttgt ttttgaactg cctgatgaag
    30481 atgtaagtgt ttttaagttg tagataaaat tgattttgcc ttgtcaggaa tataaagagg
    30541 tgaaataaat cctaagtttc atctgtcttt ttgagctttc ttttaaaatt cgagtaaata
    30601 atattttaat ttttttgaaa cagattttcc gatttatcgt cttttaaatt aaatgacggc
    30661 taaagaattt tgcaaacagg tattgccact acaggataaa atttttaggt tggcaaaaaa
    30721 gttctttaac agtgttgacg atgccgatga tgctgttcag gaagtttttc ataaactatg
    30781 gacacgaaag gaagatattg actcattcaa aagtttggag gcttttgcat tgattgttac
    30841 caggaatcat tgtcttgata caattaaggc aaaaaaacat catgttacac aattaaattt
    30901 agagcatgac atcgctgatt caacctctca acaaaagaat tttgaaaaca aggatgaact
    30961 tgttacagtc tttaagctcc taaacgaact gcctgaatta caacgaacta tttttcattt
    31021 gagggatgtt gaaggtctgg agtttgatga tattgcagaa atccttacaa tgaatccggt
    31081 tactgtaaga gtgaatttat caagagcacg caagtttatt agagaaaaaa tgattgccat
    31141 tcaaaactat gaatacaaaa gagattgaaa ttttattgga taaatatttc gaagccttaa
    31201 caacggttga cgaagaaaat attctgagaa gttattttca gagagaagat attgcagatc
    31261 atctaaaatc cgttcaatca cttttttggc taatagaaag acaaaaagct gaaaacactg
    31321 aaactgacca cggagagcga ttgatggaaa aaatttccct aaaaaaggaa aaaggcttac
    31381 accagaagcg tttgttttat ttcgcatcat ctatagcggc gatagtatta ctgtttttat
    31441 caattatcta tttcaatact aatgaaaaac ccgtaccccc agctaaatac tcacctgagg
    31501 aaatccaact ggcgatgatt caaactacca atgctctaaa ttatatttcg ttaaagctaa
    31561 ataaaggcac atcgcagctt tatcatctgc aaaaatttga acctgcttta catacgctgg
    31621 caaaaaatct atcaaagtta gatcgggaga ttgaaaaagt caataatcaa cataatccat
    31681 aatcctaaca gtgaatcaat gtcaaatcaa ttaatcaata attataattt ttttgaaatg
    31741 aaaaagatca aatttgttat tccgtttttt gcatttatgt gcttgtcact attggctgtg
    31801 ggacaattac ccacaaagag tttatatcaa aaatactcat cggtaaaagg tttttcgtta
    31861 gttaccataa acaaggacct tttcaacgtc ctttccgaaa taaatgatga aacaaaatcg
    31921 gagagtggta aagagattaa ccaaataatt aacaacttgg attacataaa catattaatg
    31981 tttgatgctc ggaagagtga tgatccaaaa tttttggaga aatttaaaga agagattaat
    32041 ttacttaatt tagctaatta tagtgagctg atgatggtaa aagaagagga agaggaggtg
    32101 aaaattttta cacttaagga aaaagaggag ataaaggagc ttgttttgtt aatttctgaa
    32161 ccacaacaag ctggctttat aagtattgca ggtaacatta atttgaaatc aatagggaaa
    32221 attgccagag cattaaatat acaaggcttg gaaaatcttg aaaagataaa tactcaagag
    32281 gaaaagtaaa attaatcttc tggcgaaaaa agcctgcttc aaggattgat ttgcaaggtt
    32341 ttaaaccgaa agaatcggtc agtaatttat gcatttcaat aatagttgaa ccaggctttt
    32401 ttatttaaca ctttatgcct ttctaagcta aaaaagccac agtatcatta gttccgagaa
    32461 taaggggtga ttttaaatat tttgaatggc ttatctatgt ttaacaatag taagctcgtc
    32521 aataatattc ttggctcctg cgaatttatc aattataaaa aggacgtaac gtatgtcaac
    32581 agagattgtt ctttgtaaat caggttcgaa gattatatcg ccgctcatgg cttcccagtt
    32641 accgtcgaag gcgagtccga tcaactctcc tcttgcattc attaccggac taccgctatt
    32701 tccgcccgtg atgtcgttat tggaaataaa accgacaatc atctctccat ttttaccata
    32761 aggcccgtaa tctcttttat tatatagctc tttaagtttt tcgggtacat aaaactccca
    32821 atttgaaggg tcttcttttt ccataacccc ggctaatgtt gagtagaaat cgtaatgtac
    32881 agcatctgcc ggataatagt cgccaacaga accgtaagtt agacgcattg tgaagtttgc
    32941 gtctggataa aatgttttac tctctttttt catctcacgc aaacctctga tgtataacct
    33001 ttcgcctttt gcagacagtt cgttaatttc gccatacctc ttccgaatag tctgctgaat
    33061 atctaaaaag gcatttccca ggagtgttgc cggatctttt tctaagactt tagccgtggg
    33121 cttgttgaga agtttgctta ccttttcctg acttgtaaaa acagattttg aaaaaacata
    33181 atttgcataa gccgaaaagt tacctttgaa tttcttgtat aatgtgttga taaatgccgg
    33241 ttgttgattt tgaggtacgt ttttataata gatagtaagc attgtgccaa gtaattcttc
    33301 atcaagcttg gcattataat ttttgaaatg attgattgat atttctttta attttttgat
    33361 ttcattatcc aaggcggtgt tatcaggtgt tttttcatta atgattttaa gcaaattatt
    33421 aaatctgcgt gaaaaaagga ctatttcact tcctcgtgtg atggcttcat tcagataaat
    33481 ttgcgcaata tttatttcac gttgtttgcc tactgcttct tttcgcattg caagcgcctc
    33541 gccatatatt ggttttcttg ccggatcgcc atttatccat gacgtaaaat ccgcctcttc
    33601 aaggcgtttc ttctcaacta cttttaacct tttcagccct cttgtttgcc caatacaata
    33661 tttccaatag ttagaagttc cggcatattt tgaagcgtat tggattctta cagcatctga
    33721 agcatccatg tatttacgca tgatattcag tttttgttct ctaataaaaa caaccgtcgg
    33781 attgctctct tcaatagcca gttcgacgtc ccaagatgaa aaataacggc ttgtgcttcc
    33841 cggatatccc atgatcatgg taaagtcttc gttttccaca ccatcaattg agataggaag
    33901 aaaatgcttt ggtttgtatg gtatattttc ttttgaataa tcggcaggtt tgccatcggg
    33961 accggaatat attctaaaaa tcgagaaatc tcctgtatga cgtggccaca tccagttatc
    34021 ggaatctgct ccatatttgc ctattgatga agggggagct ccgactaatc taacgtcttt
    34081 aaaaatttcg taaacgaaca aatagaattc atcgccggca aagaaacttc ttacgcttgc
    34141 ttcgtaatgt gtctctttag tagcttcgtc cgttattttt ttacctattt tacttatttc
    34201 gctgtttctt tccttttctg acatctggtc gtttagcata accttaactt tttcggttac
    34261 atcttcaatg cgtataagaa aagttgcagt aagtccggga tttggcaact cctcttctaa
    34321 attcatagcc cagaaaccat cggtaagata atcgtgttct atggagctat gggcctgtat
    34381 ctgtccataa ccacagtggt gattagtgaa aatcaggcca tttggcgaaa caacttcgcc
    34441 tgtacatcca cggccgaaaa tgataatagc atcttttaaa cttgaattat taatactata
    34501 tatttcttcc gccgtaagct gcaggccttt ttgttgcatg tcggtgtaat taagcctgtc
    34561 aataaacaat ggcagccaca ttccttcgtc agcataacta acaaagctta atgcgaggaa
    34621 taaagtcaat agaaatccat aaattttttt cattccgaaa tagagttgaa gttgttgaat
    34681 taaataaaat tttctccttt tttgtttttg atatcgctca caaatcttcg tatttcctgc
    34741 tcatccgatt tccggcaaat gagcagtgca ttgctgtctt ccacgataat ataatcgtct
    34801 aatccttgaa gcaccaccaa ttttccggcg ggcatattaa ttatacagtt agtggtgtcg
    34861 tacagcaaca catcatttcc taccaccgca ttgttatttt catctttttt gcgcgtgtcg
    34921 taaagcgatc cccatgtacc gaggtctgac catttgaaat cggctgataa aacgtacaca
    34981 tttgtagctt tttccatgat gccgtaatca attgatattt tttggcaact tgtataaata
    35041 tcgttgataa atgatttttc tttttcggta ttataaaggc caattccctc tttgaacaga
    35101 tcattaatgt cgtcaagata tgtttcaaat gcttttgtaa tagtttttag cgaccaaatg
    35161 aagattccgg agttccacaa aaaatcccca cttgcaagga atttttcagc taattctaaa
    35221 tttggctttt ccgtaaatgt tttcacttta cgcatatttt gattgttggg gtctctaatt
    35281 gcgtcattgt attgtatgta tccatatcct gtgtcgggac gtgttggtat gatcccaagt
    35341 gtaatcagcc attcgttttg cgatgctgct gtgagagcgg aattaatgtt atttgtaaat
    35401 tcctcttcat caataatgat gtgatcggag ggagctacta cgatattagc attggggttt
    35461 atggctgcaa ttttatagtt ggcgtaggct atgcaagggg cggtatttcg catagccggt
    35521 tcaagtaaaa tgttgttcga agccatttga ggcagttggt gttttacttg attttcatat
    35581 atctcattgg taacgataaa gatgttctct ttagggcaaa tccgttcaag gcgtttgaat
    35641 gtgctttgaa ttaaagtctt gccattacca aggatgtcaa taaattgttt cgggcgggtt
    35701 gtccggctca tcggccaaaa ccttgctcct attccgccag ccatgattac gcagtagttg
    35761 tctttattca tattagctat tttatgttta gataaagatg aaattttatt tgatgtggtt
    35821 gttagtaaaa ggtcaataaa tttagaaaaa tgccttttta aaaatcaatt taaaggataa
    35881 acttcaagta acgggctaaa aacatagagt cgctgattgt ctaaggagag gcaaaaatag
    35941 tttttgcgtc gcttctttaa tttcttaaat ctccttcctt cagcggtttt aaagattgta
    36001 ttctcaggta tggattctat tgatattcca ttcaagtctt tgtcatattt ttttagcgtg
    36061 cgtgccattt ttatgtcggc atgggttgaa gcatagatac gccggttttc atctttcaaa
    36121 taattgagta tatcatcggg gaatatttca tcagaagcca gcgctgcatg taattttttc
    36181 aattcctttt tccactcttt accgtgaggt gcaacactgc gtttatattt tgtccatgtt
    36241 atatgatgag ctaattcgtg taaaaaaacc aataaaaacc cataaggatt aagtgagcca
    36301 ttaatggaga ttcgggccgg ttttccgcta accgctgggc ggtaatctcc tgctttgctt
    36361 ttacgtgtgg gagtaatagt tagttgaata ttatttttta cgatttgatc atataccaga
    36421 tctattgcat ttttaggaag atgattgcgc aacgtttcga ttagtttata cactataata
    36481 cagagtttta aatgtgcttt tcaaaatgat taaacaaatc ggtttgatta atattggctt
    36541 tatcctcaat atgataactc catttaggac agtcgcattt ttttcgtttc gatcctcttt
    36601 tggttgaact acacgaaagc gccgtcaaaa caatgaatag
//
LOCUS       DAOOAI010000008        35803 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_1541_length_35803_cov_5.382035, whole genome shotgun sequence.
ACCESSION   DAOOAI010000008 DAOOAI010000000
VERSION     DAOOAI010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 35803)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35803)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35803
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1541_length_35803_cov_5.382035"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            364..4566
                     /locus_tag="PK345_01240"
     CDS             364..4566
                     /locus_tag="PK345_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPX33678.1"
                     /translation="MKNKVLTTGFQLIFFFIFFITINLSAQFQDRGKDPKTGKYIVVR
                     GERPPIDLDQVSQEAYEPGRINIKLNREFENLLPATKLSAGSDGYVVIGNSKLDRINQ
                     EFGVKSYKPLFEPLYDIYEKAEDFRERHKAWGLHLWFTVEIEIKSDVIAAVRQFANLK
                     EIEIAEPEYKKALSVIETTPYLSTPIQTDNPKGTTDWTPNDPQFNDQWHYKNTGQQGG
                     TPGADISLVDAWEIEKGNAQVIVAVIDGGIDYFHTDIAANMWSEIGYNFYNNTPNITP
                     LDHGTHVAGTISAVNNNAIGVSGIAGGSGNGDGVRLMSCQVFSSNTSGSGFELAFIYA
                     ADNGAAIAQNSWGYTNPNYYEQSLLDAIDYFNLNGGGNAMDGGISIFAAGNDNSSQNY
                     YPGYYSGTLSVAGTNNQDKKSWYSNYGSWVKISAPGGETNTVLQRGVLSTTIGNQYGY
                     LQGTSMACPHVSGVAALVISQAYGLLNSVEVEEILINNIDDHYGVNPTYIGQLGSGRL
                     NAFKALNAVQNVISAVKNPKNFNATPIGFNIISLSWEKNDDNNNVMIAWSSDGIFGKP
                     QNGISYSIGQTLPGGGTVIYSGNNTSFTHSNLNHSTTYYYKACSFNGLSEYSSGVLAN
                     ATTLCAPVSELPFEESFTQFITLPNCWEIIDNKSNGQVWSIGTVPSGLSGSFGNYAYV
                     NSDGYGFFGSQNTDLITPEFDFTNYTDVKLSFNHFYKHYSSSASVDYSIDNGNTWTNL
                     QTWTASTTNPTTFDQTISAIAGESQVKFRWKYIGAWSYYWCVDDISITGTWNGPIIDF
                     TANPTEVYPAETVTFTDASSNGSFTSWSWDFGEGAIPATVTGQGPHEVIYQSYGKKTV
                     SLKVDNHPIKTKAEYINVVKQIFSASETYTNGDIPTDKDFTSLPGASSCPGYLTVNIP
                     LGAEINGVDVSYNMTASSTGRKSHQRSQLRCVSPGGTNETSLAVGMGSSIGTQSYNRT
                     QLNIANEVAGGGEIAFELHAGRTTAGSGCNSFFNKVDNNSWTITVYYTLNPFYQADFA
                     ADKNSICEEEQVQFTDLSSGAPTAWEWEFPGGTPSYSNLQNPVITYNDEGIYNVTLKV
                     FYGSIIRELVKSDFIEVINCNTNILPPGWDIINTGNTDLITIPENAILTIEGEALAEN
                     NWLGVFYTNDNDELRCGGAIQVIPGQLNTLTAFGNDPVTNEKDGFDFGETYIWKLYDT
                     EAETEYPAFAFYNPEMANKNGVYGIGICEVEKLGSIQTIQVNLPESWSGFSLPFDPLE
                     QDLSLIFENIEDKLIVLKNNDYLYWPGQNINTFTNWSAYYGAEIKMSQSALLDISGVL
                     FSKTIDLINGWNYLPVLSQSPILTSDIYTALNEKVEIIKEIAGYNIYWNDMNIFTLDQ
                     LQPGKAYMIKLKQPAQLEFSFQ"
     gene            complement(4779..5450)
                     /gene="ung"
                     /locus_tag="PK345_01245"
     CDS             complement(4779..5450)
                     /gene="ung"
                     /locus_tag="PK345_01245"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009292592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HPX33679.1"
                     /translation="MNPKIQESWKLMLKDQFESSYFVQLKEFLIDEKLHYRVFPSGKN
                     IFAAFDLTPFDKVKVVILGQDPYHGVGQAHGLCFSVPDDVAQPPSLQNIFKELQNDLG
                     LPIPKSGNLTQWAKQGVLLLNATLTVRAHMAGSHQNKGWEQFTDAVISKLSKYRDHLV
                     FLFWGNYAIAKENLIDTNRHLVLKSPHPSPLSASRGFFNNHHFSKTNEYLAQHGIEPI
                     NWRIE"
     gene            5769..6554
                     /locus_tag="PK345_01250"
     CDS             5769..6554
                     /locus_tag="PK345_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33680.1"
                     /translation="MFYSKHILLLFLALFISTEIFCQTDDLDIDRFDTTLNRVILRYE
                     KSGGLLVHTGGFGAIFRKAHNVNYFRKDLWEIEFTGMQSEKQVRINLYGTYYSNANSY
                     IYGKLNKVYMLHTGLGQQHLLNSKPYWGGVEVRFTYYGGASLAIAKPIYLYIIIDQTN
                     FNFLELESQRYDPEKHFIDNIYGRAPFLDGLGKTKFYPGIYGKAGLNFEYGEYNQMLK
                     SLELGIMAEGYPIPIPMMAFSPSRYHFINFYLNVTFGKRFNKY"
     gene            6561..7127
                     /gene="thpR"
                     /locus_tag="PK345_01255"
     CDS             6561..7127
                     /gene="thpR"
                     /locus_tag="PK345_01255"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HPX33681.1"
                     /translation="MQRLFAAIKVIPEVKLLSNYRELKSLLHNDKITWVGEQNIHITL
                     KFFGDTPESDIPTIENILSQVASGHHPFVLNLESIGIFGSSYNPRVIWLGIKEPESVN
                     ALAEDILNTLDKSGYPRDDQHFRPHLTIGRVKMLKFKKDFQNIINRFKSTYFQEVQVN
                     SFELFESKLTPQGPIYTKLQTFYLGRNG"
     gene            complement(7216..7644)
                     /locus_tag="PK345_01260"
     CDS             complement(7216..7644)
                     /locus_tag="PK345_01260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33682.1"
                     /translation="MADNTLFVIQLYDYPVKILLALEMAISGKHEFARWLLDNNYPEL
                     AALANAIRGDKKAIEWLLDHGFQHFAAFDAASVDDDQALAWLKRYDFEFLVILAQASR
                     GDQDSINWLNNRELKIFVRIAKQIKFFVDGQTFDYHKMPF"
     gene            complement(7736..8515)
                     /locus_tag="PK345_01265"
     CDS             complement(7736..8515)
                     /locus_tag="PK345_01265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008778938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPX33683.1"
                     /translation="MAGFLFSETIFGPVRSRRLGNSLGINLLPNTHKICTFDCIYCEC
                     GWSPDESDYFGKLPTRNEVAEQLEVKLLNLKANKNFPDSITFAGNGEPTIHPEFSGII
                     HDTIKLRDKFFPEADVTVLSNSSMIHKPEIFNALNQVDNNILKLDAGFDSTFRLINRP
                     RSKELNLDVIVSNLRKFGKKAIIQTLFVQGEFKGRAVDNTTDAEVAEWINHIKLIKPR
                     YVMLYPIDRQTPTLGLKVVGKDKLLAIAEKLKEIGVEYKVY"
     gene            8635..9609
                     /locus_tag="PK345_01270"
     CDS             8635..9609
                     /locus_tag="PK345_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPX33684.1"
                     /translation="MANIKEIKEPINDHLLAFEKKFEKAMKTRVALLDYITRYIIRRK
                     GKQMRPMFVFFSAGITGQITESTYRAASMIELLHTATLIHDDVVDDSNIRRGYFTINA
                     LWKNKIAVLVGDFLLSKGLLLALDHNEFELLKIVSVATREMSEGELLQIEKAHKLDIE
                     EGVYFEIIRKKTASLIASCCAAGAHSAGADKETIKKMYQIGEHIGMAFQIKDDLFDYQ
                     KNKEVGKPNGIDIKEQKMTLPLIYLLNNSSFLEKKKVINIVKRHNLNAEKVDRVIEQV
                     NKSQGIEYATRKMYEYRDKAFELLLDFPESPMRKGFEQLVIYTTERNK"
     gene            complement(9670..11727)
                     /gene="metG"
                     /locus_tag="PK345_01275"
     CDS             complement(9670..11727)
                     /gene="metG"
                     /locus_tag="PK345_01275"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HPX33685.1"
                     /translation="MQNKKQFKRYTVTSALPYANGPLHIGHLAGVYVPADLYVRYLRS
                     NEKDVVFIGGSDEHGVPITIKAWQQGVTPQDIVDKYHELIKKSFAEFGISFDIYSRTS
                     NKIHHETSSEMFKKMYDEGKFIEKYTEQYYDESHQTFLSDRYIIGTCPICSYEKAYGD
                     QCENCGTSLNATDLINPRSVLSGKEPVLRKTKHWYLPLDQYTDWLKEWILEGHKVDWK
                     TNVYGQVKSWIDQGLQPRAVTRDLNWGVKVPLSEAEGKVLYVWFDAPIGYISATREWA
                     EQKGVEWEKWWKDPETRLIHFIGKDNIVFHCIIFPIMLKAEGSYIVPDNVPAFEFLNL
                     EGQKISTSNNWAVWLHEYLVEFKGKQDVLRYVLLANAPETKDNDFTWKDFQTRNNSEL
                     VAILGNFVNRTLVLTHKYFDALVPQASQFNEDDLKAIAEIEAIPERISMSLENFRFRE
                     ALAEMMNLARLGNKYLTDNEPWKIFKSDPDRVKSVLNISLQICANLTIVAAPFLPFTA
                     KKLSEMLNIGQMKWKEAGNIHLLKPGHRINNPELLFEKIEDETINAQIEKLNATSRAN
                     QNDKFVPNPLKEPVQYDEFAKMDIRTGTILEAEKVAKTKKLMKLTIDTGIDKRTVVSG
                     IAEHYDAKDLIGRKVCILVNLAPKELRGITSHGMILMAENPDGSLGFVAPPDNYVDGT
                     EIK"
     gene            12062..13615
                     /locus_tag="PK345_01280"
     CDS             12062..13615
                     /locus_tag="PK345_01280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33686.1"
                     /translation="MRYGWLLLILLWINSFAVHAQSNSGPVRVELEARAQVFNLVPCN
                     DKGIMVFYETINQPDQQNKTWFFILHNTALETIWTREISINVDFVFQSSFIDNENLFL
                     VFQKSTKAKTDSHFFQLLNLNLQNGESVTYSIPMPANSDIVTFEIFSNHLILGLNHGK
                     EQATLLIKDLSTGDESTVKFNDQSTFIKDLKIDAATSTIYTALNIFQSKRESSFYVNA
                     YNMSGQLLQSIALTPPQTTERIMNGQLHIDNEKNIYVIGTYNNTNGKAIKEDRTKSGD
                     PSEGFYMAGIVGDSQKFLHTYSLLNFSNIRRILNNKQLAAAESLLKKQNRQNKEQGLT
                     YDFLVHNLQVNDDELIIAADAFYPEYRQVSTMSYDFYGRPMPYYYTIFEGYQYFNAFV
                     AGFNKQGELGWSNGIKIWEMKSFQINQKTEVFIDDDEMFIFYNHDGKIVSKAFHKFND
                     IGTIENTQIATNFPGDRLIENSQGNIKHWYGNNFVAYGYQTLQNNTQGSSERRKVFYM
                     NKIALDYQY"
     gene            complement(13612..14706)
                     /locus_tag="PK345_01285"
     CDS             complement(13612..14706)
                     /locus_tag="PK345_01285"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HPX33687.1"
                     /translation="MIPLPSSFDFFDVINNFSIEDKILKIETIGEGHINDTFFLKTNT
                     NRNYLLQRINHLIFKEVEALMDNISRVTSHINQSNLSNSAKAILRAPTVIFTKDNKSF
                     FLDIFGNYWRCYTYIPHEPIKRITPAIAMEGGKAIGMFQTILSTLPGNQLYETIPDFH
                     NLKMRLKALYKAVEENNYGRLKQVEKEVDFIFAREGELLRIHKLESNGLIPIRVTHND
                     TKFNNILFDKGRSAVAIVDLDTVMNGSVLYDFGDAIRTLCNTAVEDESDLTKVNFNKK
                     LFEAFVNGYLSITSATLKHVEVDLLAFSCKLLTYIMAVRFLTDFLNGDVYFKVKSQIH
                     NYQRAASQLKFLECIEKDFKWMEKVIRDKA"
     gene            complement(14713..15618)
                     /locus_tag="PK345_01290"
     CDS             complement(14713..15618)
                     /locus_tag="PK345_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HPX33688.1"
                     /translation="MKKPTLLILAAGIGSRYGGLKQIDKIGPSGEAIIDYSIFDAMRA
                     GFGKVVMVIRKELEADFRDFFNDKIKGKLNIQYVYQELDNLPEGVVAPAGRVKPWGTA
                     HAVLVASEVINEPFMVINADDFYGMEAYQSIVQYFTTNNNLKKHCMVGYQLQYTLSDH
                     GTVSRGICSYGEDMILKGIREVTNVERRSDEIGYNHPKQGWSTIEDDTIVSMNAWGFY
                     PGIFQLFKKGFADFITNADDILKAEYYIAKPLAWMIEKKLGDIQVLESDAKWFGVTYR
                     EDKELAKEKINKLINSGLYPENLWS"
     gene            complement(15676..17529)
                     /locus_tag="PK345_01295"
     CDS             complement(15676..17529)
                     /locus_tag="PK345_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+:solute symporter"
                     /protein_id="HPX33689.1"
                     /translation="MVLTSIDWVIITLFFVVLIVIAIFSSRFAGRGYNEFFLSGRNMP
                     WWLLGVSMVATTFSADTPNLVTDIVRQNGVAGNWAWWAFLLTGMLTVFIYAKLWNRSQ
                     VLTDLEFYEIRYSGKMAAFLRGFRAIYLGFFFNVMVIASVSLAFVKLAGVMMGVPPAP
                     ALIFAAIIVVFYSALGGLKSILWTDLFQFGFAMFGAIIAAVFVVKGLPENVHGLSGLL
                     SHENVAGKLSLFPKLTRTDMFLYIFIIPIAVQWWAVWYPGAEPGGGGYIAQRMLSAKE
                     ERHAMGATLLFNFFHYALRPWPWIIVALASLVIFPDIQSMVSAFPGVAEQYIKEDFAY
                     PAMLRTFLPAGLLGLVVASLIAAFMSTVASHLNWGSSYLVNDFYNRFVNPKASEKQKV
                     MIGRVSSVLLMVFGVLLALVLENALQAFQYILMIGAGTGLIYILRWFWWRINAFSEIS
                     AMIAATIFSLVFIIIENFFLKIVPEMPGYVQVFGITMSEIYWNILKLVGVVVLTSISW
                     ITVTYLTKPVRHATLRSFYEKIKPGGPGWKKVLKDAELSGNPIDPEMSKSWDVPTGIL
                     CMLLGSVAIYSALFATGHWIYGNNVTASLLTGLAVVSAFILTKAWKRLTFN"
     gene            17810..18715
                     /locus_tag="PK345_01300"
     CDS             17810..18715
                     /locus_tag="PK345_01300"
                     /inference="COORDINATES: protein motif:HMM:NF014114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HPX33690.1"
                     /translation="MITPSNLHKGDLIGLISPSAKIDPSILYSATRLLEAKGYRVKQG
                     EHALSQHYYFAGTDSQRAHDFQLMLDDPSIKMIICNRGGYGMVKIIDRLDFSCFLDNP
                     KWIAGYSDITVLHCHLAMLYKTKSIHSIMPYDFPADGNSCLAIDKLLEVAGGKKPTYN
                     LPTHDLNLEGSANGMLVGGNISILTSLLGSQSELDTEGSILFLEEVGEPLYRLDRMMQ
                     TLKRSGKLSSLAGLIVGGMTKMNEEQEDYGLSAEEVIMDMISDVNYPVCFGFPAGHFP
                     ENHPLIIGGEVSLSVNKNSVKIIFK"
     gene            18729..19091
                     /locus_tag="PK345_01305"
     CDS             18729..19091
                     /locus_tag="PK345_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HPX33691.1"
                     /translation="MADHNITGNIGEELAEKYLRSLGYEILEKNWRYGKNEIDIIARD
                     DRFLVIVEVKTRKSIYYGEPEEFVNRSKQRSLIAAANGYISKKNIDLETRFDLVSVIF
                     SGNKHTIRQIKDAFYPIV"
     gene            complement(19099..20016)
                     /locus_tag="PK345_01310"
     CDS             complement(19099..20016)
                     /locus_tag="PK345_01310"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HPX33692.1"
                     /translation="MTFVTKEKLFSREWFKVYGLIFFGTFIMSAGYVLFINPYRVVPG
                     GVIGISIVIHYLTEGVFSFWPTGIPIGLMALTIDIPLALLGVKILGPRFGIKTVVGFV
                     LTATFMDVLTYFIGQDDPLNLGDQILLASIFGGVLIGFGLGLIFRSRATTGGTDLISM
                     MINKFTGVPLGQLLIIVDSIIVLLGLIAFGDWKIPLYSWIVIFITGQVVDITLRGISY
                     EKMMFIISDNHEEIRQKIIFDLNRGGTIFVGRGMFQNNERNVIYTNVSRREYQILKDF
                     IREIDPRAFITVVDAYEVLGEGFKSFHDH"
     gene            20305..21147
                     /locus_tag="PK345_01315"
     CDS             20305..21147
                     /locus_tag="PK345_01315"
                     /EC_number="1.8.4.11"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methionine sulfoxide reductase B/A
                     protein"
                     /protein_id="HPX33693.1"
                     /translation="MNYKSLTPDEQYVIVNKGTERPFSGKYNNHHEKGIYKCKQCGNA
                     LYSSEDKFDSGCGWPSFDDEIPGAIKKVADADGQRTEIVCAKCNGHLGHVFVGEGFTP
                     KNSRHCVNSISLDFISEAKTSKAIFASGCFWGVQYHFNKEKGVIKTLAGYTGGHVKNP
                     TYEEVCTGTTGHAEAVEVIFDTALTDYETLLKLYFETHNFTQIGGQGPDIGEQYRSVI
                     FYTDNQQKAIAEKLIKHLEEKNYKVATKLEKATQFYPAEEYHQDYYFKRGGSPYCHIK
                     KKVF"
     gene            complement(21378..21941)
                     /gene="pth"
                     /locus_tag="PK345_01320"
     CDS             complement(21378..21941)
                     /gene="pth"
                     /locus_tag="PK345_01320"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005841628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HPX33694.1"
                     /translation="MKYLIAGLGNIGEDYLNTRHNIGFIILDALAQEYKTIFSVQRHA
                     YLAEIKIKGRHVYLIKPTTYMNLSGKAIKYWIEKENIPIENLLVVLDDVDLDTGILRL
                     RQKGSGGTHNGLNHIIETLEHTEFSRLRVGIGKDFAKGFQVEYVLGKWTKSEEEIMLR
                     QIPVAVDMIKSFVLAGPRITMNQYNNK"
     gene            complement(22022..22651)
                     /locus_tag="PK345_01325"
     CDS             complement(22022..22651)
                     /locus_tag="PK345_01325"
                     /inference="COORDINATES: protein motif:HMM:NF004132.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HPX33695.1"
                     /translation="MKSVSLSGSRRENVGKKDAKKLRRDGNVPCVLYGGEEQIHFAVP
                     AKSFKKIIFTPNSYIIELEIDGKKILSILKDVQYHPVSDEILHVDFLQIFEDKPVVIS
                     VPVNYSGIAKGVLKGGRLVRKYRKLKIKAFLKHLPDEIVVDITNLNVNDSRKVMDLKL
                     DNVEFLDSPSSIVVAVKSVRTVVEEEEEVAEEGAEAKATDAPDAPEAAN"
     gene            complement(22684..23625)
                     /locus_tag="PK345_01330"
     CDS             complement(22684..23625)
                     /locus_tag="PK345_01330"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate pyrophosphokinase"
                     /protein_id="HPX33696.1"
                     /translation="MAKNVNIFSGDSSKYLADKIAEAYGQPLGKVTIQHFQDGEYQPC
                     FEETIRGNDVFIIQSTNAPADNLLQLLLLIDAAKRASAQRVVAMIPYFGFARQDRKDK
                     PRVSIGAKLMTNLLVAAGVDRIITMDLHSDQIQGFVDIPVDHMYASSIFVPHLKKMDL
                     PNLTMASPDTGGTKRAAAYAKFLNTDLVICFKHREVANKVSSLRIIGDVKDKDIVLVD
                     DIIDTAGTITKAANMMMDMGASSVRAVCTHPVFSGDAEEKIEKSALTEVLVSDTLERQ
                     QKGKITVLSTAEIFADVIKRVHKNKSISTHFDFLAMI"
     gene            23807..23892
                     /locus_tag="PK345_01335"
     tRNA            23807..23892
                     /locus_tag="PK345_01335"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:23841..23843,aa:Leu,seq:caa)
     gene            24073..25203
                     /gene="tgt"
                     /locus_tag="PK345_01340"
     CDS             24073..25203
                     /gene="tgt"
                     /locus_tag="PK345_01340"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HPX33697.1"
                     /translation="MLFSLDTIDPKTSARAGTITTDHGTIQTPVFMPVGTGGTVKAIH
                     YRDLYEDSKAEIILGNTYHLYLRPGLGILEQAGGLHEFIGWKRPILTDSGGYQVFSLS
                     AQRKIKDDGVHFRSHIDGSKHLFSPESVIDAQRIIGADIMMAFDECTPWPCQYEYAAK
                     SLLLTHRWLDRCLHHLNNTEPKYGYQQALFPIVQGSTYADLRKQSAEYIATKNAAGNA
                     IGGLSVGEPADLMYEMTEMVCNILPHDRPRYLMGVGTPVNILESIALGIDMFDCVMPT
                     RNGRNAMLFTSEGIINIKNEKWKNDFSPIDPNGTSFVDHAYTRAFLRHLFVSQEYLAP
                     MIASLHNVTFYIWLVKTARAKIIEGTFSVWKDAMIPKLNNRL"
     gene            25243..26322
                     /locus_tag="PK345_01345"
     CDS             25243..26322
                     /locus_tag="PK345_01345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPX33698.1"
                     /translation="MKIIDRYIIRKFLGTFLYSIALLSVVIIIFDLSEKIDDFLEKEA
                     PLRIIIFDYYLNFIPFFVNMFSYLFTFISVIFFTSKMASNTEIVAILSSGISFKRFLW
                     PYIFSATIIGLLSLFLANFVIPNTNKTLWEFEKKYIKNPIRNEDMNIHMQINPGVFIY
                     VESFNTSTKTGQKFSLEQFDNKGNLVYKLNSDYFSWVPETGKWRIKNYYTRTIDNNKE
                     VFIKGLEIDTTLNLHPSEFTIISDDIKTMDYFELKAFIEKERLRGSPKINEYNVEQYR
                     RYAFPFSTLILTLIGVSLSSRKVRGGIGMHLGIGIGLTFSYILFMQVSQVFAAVGGIS
                     PMLAVWIPNIIYGLLSIYMLKIAPK"
     gene            complement(26343..26813)
                     /locus_tag="PK345_01350"
     CDS             complement(26343..26813)
                     /locus_tag="PK345_01350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33699.1"
                     /translation="MEDESGPYKYSLDELIDLLKRIMADVDKSQYENLDPIIKQSIDF
                     IIGNDGDSSERPFPEFLKGMGIFSFNKWITDVISAVKDSLNIDFDIHTDDLLHKDEEF
                     KYQPSDKNKKIDVDERIRLIDEMLKRPELTEQAIDNLLDERNQLLNLKKVKSEK"
     gene            complement(26935..28308)
                     /locus_tag="PK345_01355"
     CDS             complement(26935..28308)
                     /locus_tag="PK345_01355"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HPX33700.1"
                     /translation="MEHVKKYIDANKDRFLSELFELIRIPSISSIQDHKDDMLRTAEL
                     WKKFIIEAGADRAEVMPSEGNPVVYGEKIVDPNKPTVLVYGHYDVMPVDPIEAWESDP
                     FKPEIRDGKIFARGADDDKGQAFMHAKAFELMVKTNTLPCNVKFMIEGEEEIGSPSLG
                     NFCKKHKELLKADIILVSDTTMISTDIPSITTGLRGLAYMEVEVTGPNRDLHSGLYGG
                     AVANPINVLSRMIAQLQDENFRITIPGFYDDVIEVMPAERAEMAKAPFDLDEYKKSLD
                     IEEEEGEAGFSTMERTGIRPSLDVNGIWGGYIGEGAKTVIPSKAYAKISMRLVPNQDN
                     KKIAQLFANYFESLAPKSVKVKVTELHGGYPYVSPTDMHAYQAAEKAYTATFGKKPIP
                     VRSGGSIPIISTFEKILGLKSILMGFGLESDAIHSPNENYPLFNFYKGIETIPYFYKY
                     FTEIMLA"
     gene            28429..29445
                     /gene="gap"
                     /locus_tag="PK345_01360"
     CDS             28429..29445
                     /gene="gap"
                     /locus_tag="PK345_01360"
                     /inference="COORDINATES: protein motif:HMM:TIGR01534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HPX33701.1"
                     /translation="MKKIRIAINGFGRIGRTFCRQMQYNKGLELVAINDLADSYTLCH
                     LLKYDSVHRAFPGNVTCDPSHLIINGHKVSILNIAQPALIPWKEYDIDVVVESTGNFT
                     KKEDAEQHLAAGARKVVISAPAKGENDDIQYVVLGVNDHLINWDVPIISNASCTTNNV
                     APLIMILDENFGIEEGFITTVHSYTRDQHLLDAPHKDLRRGRAAAYSIVPTTTGAAKA
                     STKIFPHLTSKLGGAGIRVPVPDGSLTDFTCTVRKSTTINEINQHFKQAADGKLKGIL
                     QYTEDPIVSTDVIGNPYSAVFDAQLTAVLGQKNRLVKVVAWYDNEIGYSSRLVELIEK
                     FAGK"
     gene            29687..30541
                     /locus_tag="PK345_01365"
     CDS             29687..30541
                     /locus_tag="PK345_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol acyltransferase"
                     /protein_id="HPX33702.1"
                     /translation="MKPEISKEFINLEKLFQDKNPKLYRLIPTFVFKYLRRVIHQDEI
                     NANLYEFRDLHGIDFIKAVLNRFGVIIHSKDLKNLPATGRYIIVSNHPLGGLDGMALM
                     QEIGKVRTDFLFPVNDLLMNLPNLKQLFIPINKHGSNTENIQLFNDTFASDILLLYFP
                     AGLVSRKQGKEIIDLEWKKTFLSKAIQYQRDIIPVHISGQNTNFFYNLANWRKKLGIK
                     ANIEMLYLVDEMFKQKDKTITITFGTPIPIKVFDKRYSNNQWAKLLKEHVYKLGSGDE
                     SVFKYITN"
     gene            30567..31487
                     /locus_tag="PK345_01370"
     CDS             30567..31487
                     /locus_tag="PK345_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008863185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX33703.1"
                     /translation="MEIIIPQPDKSLLLKELTPERFVRHTNFGKNEIYIFNSNNSPNL
                     MQEVGRLRELSFRMAGGGTGKSVDIDQYDLGSKPFEQLIVWNPVNQEIVGGYRFVLMK
                     KLGVDSSGKVKTPTSKLFYLSQKFKSDYLPFTIELGRSFVQPDYQPSKNPRIGIYSLD
                     NLWDGLGAIIKLNPEVKYYFGKITMYPKTDLLAREAVMYFLGKFFPDKEKLAYPINAV
                     NLQTDVAFFENLFNKDSYAENYKILQQFVRKQYANIPPLVNAYMNLSLTMKTFGTTIN
                     STFGNVEETGIMITISDIHPEKKERHLTGM"
     gene            31501..32130
                     /gene="yihA"
                     /locus_tag="PK345_01375"
     CDS             31501..32130
                     /gene="yihA"
                     /locus_tag="PK345_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005777046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HPX33704.1"
                     /translation="MEIKNATFIISNTDYSKCPTPTLPEYAFIGRSNVGKSSLINMLC
                     SRKGLARISVQPGKTQTINHFLINETWYLVDLPGYGYAKISKTTREQWKTMINNYLTK
                     RENLVYTFLLIDIRISPQNNDLDMINNFGRWQLPFAIVFTKEDKLKPTEAKANIERYQ
                     IELTKSWEELPPLFITSSVSGAGKDEILNFISENNDYFFKWKKMPFTKR"
     gene            complement(32200..32523)
                     /locus_tag="PK345_01380"
     CDS             complement(32200..32523)
                     /locus_tag="PK345_01380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein subunit"
                     /protein_id="HPX33705.1"
                     /translation="MEKETNPKYRTLILDDVKYRTLLTKKFEKRKPYVPDDPSKLKAF
                     ISGTIRKVFVKKGTKVERNDKLLILEAMKMKNIIYAPFDAKIKKVYVKEGEMVPKSAL
                     LIEFE"
     gene            complement(32587..34134)
                     /locus_tag="PK345_01385"
     CDS             complement(32587..34134)
                     /locus_tag="PK345_01385"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008147311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPX33706.1"
                     /translation="MSIKTKSTDLRRRMRDALQGGGTQAIEKQKATGKLTARERIIAL
                     LDPKSFHEYDLFVEHAGKDFDMDKKYLPGDGVVTGTGTINGYPVCIYAQDFTVAGGSL
                     GFMHAKKITKIMDHAMKLKVPIIGINDSGGARIQEGVNSLAGYGDIFYRNTQASGVIP
                     QISVILGPCAGGAVYSPALTDFVFVVDKISKMFITGPEVIKTVLGEEISMEELGGARV
                     HAEITGNAHFFAESEQECFDQIKQLVSFIPWNNIKKADTFPKKNPKTRKYKIEEIIPS
                     DPKTPYDVRDVIRAIVDDSEFFEIQELYAANIVIGFARLEGQTVGFVANQPMVLAGVL
                     DVDSSDKAARFIRYCNAFNIPLCTLVDLPGYLPGIEQEHAGVIRHGAKVLYAYSEATV
                     PKITLIMRKAYGGGYIAMCSHHLKADFVFAWPTAEIAVMGPEGAANIIFRKEINSAEN
                     PEEMRRQKIKEYKKKFANPYVAAAYGYIDAVIEPAETRIFLAHALEISNNKTEIPPHK
                     KHGIPPF"
     gene            34505..35056
                     /locus_tag="PK345_01390"
     CDS             34505..35056
                     /locus_tag="PK345_01390"
                     /inference="COORDINATES: protein motif:HMM:NF012774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HPX33707.1"
                     /translation="MKKIFFYLSLSLMILNISCLSKNRKTAYLQSKDTISGKVIAVHD
                     GDTYDLLVQGNEKLSVRMEGIDAPERGMPFYQVSKRHLSSLCSGKQVTLKITGTDNYG
                     RALGFTYLEDGRELGQEMIKAGLAWHYKKYNSDTALANFEKEARRLKKGLWLDKNPKA
                     PWEVRRLRRQGVSAKDSFNISPH"
     gene            complement(35533..>35803)
                     /locus_tag="PK345_01395"
     CDS             complement(35533..>35803)
                     /locus_tag="PK345_01395"
                     /inference="COORDINATES: protein motif:HMM:NF021915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPX33708.1"
                     /translation="KNALVKQFKRLFEMDNIELSFTDGALTTIVEKAIEFKLGARGLR
                     TIMETILNEDMFNLPSDSLTKELKITKSYVLERLEKNNFGKLQVA"
BASE COUNT        11553 a         7135 c         6382 g        10733 t
ORIGIN      
        1 aaataaaaaa agagcgacat ttttcttacc gactaatcaa atagctgtat aaaacgagta
       61 ttaataaacc taaaaatgca caggttttta ttagattgac atgaaacgct tattaaaaat
      121 gaaaaaaata attcaaaata aaaattcatt cttcataaaa tcaatttgat cattactttt
      181 aaacaataac agacttttgt taatgaagtt aatattaata ataacacgat gtttatgaac
      241 ttacaacatt cgttaagaga attatttcaa aaaaacctgt ggaaaaagca tacttttgtc
      301 aatttaatct gtttttcttc taaagcttga taataacatt tactaaaatt atatctcact
      361 aaaatgaaaa acaaagtact aacaacaggg tttcaactta tatttttttt catctttttt
      421 attacgatca acctttcagc tcaatttcag gatcggggta aagaccctaa aaccggtaaa
      481 tatattgtgg tccgtggcga gcgtccaccg attgatttag atcaggtatc acaggaggca
      541 tatgagccgg gccgaataaa catcaaacta aatcgggaat ttgaaaacct gcttccagca
      601 acaaaacttt ctgcaggttc tgatggatac gttgttatcg gaaacagcaa gctcgataga
      661 atcaatcaag agtttggcgt caaaagttac aagcccttgt ttgaaccact ttatgacata
      721 tacgagaaag ccgaagattt tcgtgaaagg cataaagcat gggggcttca tctctggttt
      781 acagtagaaa ttgaaataaa aagcgatgtt atcgctgccg taaggcaatt tgcaaacctg
      841 aaagaaattg aaattgcaga accagaatat aagaaggcat tgtcagttat agaaacaact
      901 ccttatcttt ccactccgat tcaaacagat aatccaaaag gcacaacaga ttggacacca
      961 aatgatccac aattcaacga tcaatggcat tataaaaata ccgggcagca aggcggtact
     1021 cctggtgccg acataagtct tgttgacgca tgggagattg aaaaaggaaa cgcacaagta
     1081 attgtagcag ttatagacgg cgggattgat tatttccaca ccgacatagc agcaaacatg
     1141 tggtcggaaa ttggttacaa cttttataat aacacaccca atatcacacc tttagatcat
     1201 ggaacccatg tagccggaac tatttcagcg gttaataata acgctatcgg agtgtcgggt
     1261 atcgcaggcg gatcagggaa tggtgacggt gttaggttaa tgtcgtgtca agtgttttca
     1321 tctaacacat ctggttctgg atttgaactc gcatttatct atgctgccga caacggcgcc
     1381 gctattgcac aaaacagttg gggctataca aatcccaatt attatgaaca atctttactt
     1441 gacgcaatcg actactttaa cctaaatggt ggcggtaacg ctatggatgg aggaatttca
     1501 atatttgcag caggaaacga taactcatcc caaaactatt atcccggata ttattccggc
     1561 acattgtcag tagctggaac caataatcag gacaaaaaat catggtattc caattatgga
     1621 tcgtgggtaa aaatatcggc tcccggtggt gaaaccaata cagttttaca aaggggtgta
     1681 cttagtacca caataggtaa ccaatatgga tacttacaag gaacttctat ggcttgtcca
     1741 catgtatccg gagtagcggc tctcgtcatc tcacaggctt acgggctgtt aaattctgtc
     1801 gaagtggaag aaatcctgat aaacaatatt gatgaccatt atggagtaaa ccccacttat
     1861 attgggcagt taggatccgg aagattaaat gcatttaaag ctttgaatgc tgtacaaaat
     1921 gtaataagcg cagtaaaaaa tcctaaaaat ttcaacgcca ccccgatcgg cttcaatatt
     1981 atcagcctat catgggaaaa aaatgatgat aacaataatg taatgatagc gtggtcgtca
     2041 gacggaatat tcggcaagcc tcaaaatgga attagctatt ccattggcca aaccttgccg
     2101 ggtggtggca cggtaatcta ttcagggaat aacacaagtt ttactcactc caatcttaat
     2161 cattctacca cctattacta taaagcttgt tcatttaatg ggctttctga atattcaagc
     2221 ggcgtattag caaatgcaac taccctttgt gcgcctgttt cagaattacc tttcgaagag
     2281 agctttacac agtttataac tctaccaaat tgttgggaaa tcattgataa taaaagcaat
     2341 gggcaagtct ggtcaatcgg aactgttcca agcggcttat caggatcatt cggcaactat
     2401 gcttatgtaa atagtgatgg ttatggattc tttggctctc aaaacaccga tttaataacc
     2461 ccggaatttg attttaccaa ttataccgat gtaaaactta gtttcaacca tttttataaa
     2521 cattattcat catctgcatc tgttgactat agtattgata acggaaatac atggactaat
     2581 ttgcaaacat ggactgcctc cactacaaac ccaaccactt tcgaccaaac tatatcagcc
     2641 attgcaggtg aatcacaggt caaattcaga tggaaatata ttggagcgtg gtcatattat
     2701 tggtgtgttg atgacatctc cataaccgga acctggaacg gaccaataat tgacttcact
     2761 gccaatccaa cagaagtata tcccgcagaa acagtaactt ttactgatgc ttcatcaaat
     2821 ggaagtttta catcatggag ttgggatttt ggcgaaggcg ctattccggc aaccgtcaca
     2881 ggacaaggac cacatgaagt tatttatcaa tcctatggca aaaagaccgt atcactcaaa
     2941 gtagataatc atccaataaa aactaaagct gaatatataa acgtagttaa acaaatcttt
     3001 tccgcatcag aaacttacac aaatggagat atcccgacgg ataaagattt tacctctttg
     3061 cccggcgcct catcctgccc aggctacctt acggtgaaca tacccttggg cgctgaaatt
     3121 aacggcgtag atgtcagtta caacatgact gccagcagta ctggacgaaa atcacatcag
     3181 cgctcacaat taaggtgtgt atcaccaggc ggcaccaatg aaacctcgct ggcagtcggg
     3241 atgggcagct caataggaac acaaagctat aacagaactc agcttaatat cgccaatgag
     3301 gttgctggag gtggagagat agcttttgaa ctgcacgcag gtagaacaac agcaggatca
     3361 ggatgcaata gttttttcaa caaagttgac aacaactcgt ggacgattac agtatattat
     3421 acacttaatc cattttatca ggctgatttt gctgccgata aaaactcaat ttgcgaagaa
     3481 gaacaagtgc aatttaccga tttatcatct ggcgccccca ctgcatggga gtgggaattt
     3541 ccgggaggta caccgtcata ttctaacttg caaaatccgg taataacata caacgacgaa
     3601 ggaatttaca acgttacctt gaaagtcttc tatggcagca taataagaga gcttgttaaa
     3661 tcagatttta ttgaagtgat caactgcaat actaatatcc ttccacctgg ttgggatatt
     3721 atcaacacag gcaataccga cctgattacc atccctgaaa atgccatact aacaatcgaa
     3781 ggagaagctc ttgccgaaaa taattggtta ggtgtctttt ataccaacga taatgatgaa
     3841 ctaaggtgtg gaggtgcaat tcaagtcatt cccggccagt taaatacttt gactgccttt
     3901 ggcaacgacc cggtaacaaa tgagaaagat ggatttgact ttggtgaaac atatatatgg
     3961 aaattatatg atactgaagc ggaaacggag tatccagcat ttgcatttta caaccctgaa
     4021 atggctaata aaaatggtgt gtatggcatt ggaatttgtg aagttgaaaa attaggctca
     4081 attcaaacca tacaggtaaa tcttccggaa agttggagtg ggttttccct tccattcgat
     4141 ccattagaac aagatttatc tctgattttt gaaaatattg aagataaatt gatagtatta
     4201 aaaaacaatg actatctcta ttggcctgga caaaatatta atacatttac taactggtca
     4261 gcctattatg gagcagaaat aaagatgagc caaagcgctt tactcgatat ctcaggtgtt
     4321 ctgtttagta aaaccataga tttaataaac ggttggaatt atctgccggt attgagccaa
     4381 tccccgattt taacatctga catttacaca gctttaaatg aaaaagttga gattattaaa
     4441 gaaattgccg gatataacat ctattggaat gatatgaaca tttttacttt agatcaactt
     4501 caaccaggca aagcctatat gataaaactt aagcagccag cgcaacttga gttttctttt
     4561 cagtaaataa atcacgattt agtgtttgtc tggcagataa gaattgttat tcacggggtg
     4621 gctttatact ggattatcgc tcacggggtg actttgaggc accctgtgag ttaaagaatc
     4681 accccgagaa cagccttatt caaaagaagt tttatcggac aacagtgaat tcaaaatctt
     4741 tataggcaat cccatttttt tatagtacct cttgctctct actcaatcct ccagtttatt
     4801 ggttcgatac catgttgggc aagatattca tttgttttag aaaaatgatg attattaaaa
     4861 aatccacggc ttgccgataa aggagagggg tgtggcgatt tgaggacgag atgcctattg
     4921 gtgtcaatca gattttcttt tgcaatggca tagttacccc aaaacaaaaa gactaaatgg
     4981 tctctgtatt tcgagagttt agatattaca gcatccgtaa actgttccca gcctttgttt
     5041 tgatgcgagc ctgccatgtg ggctcttaca gtaagagttg cattgagcaa aagtacacct
     5101 tgctttgccc attgagtcaa gtttccgctt ttcgggatcg gaagtccaag atcattttgc
     5161 aattctttaa agatgttttg caacgatggc ggctgcgcta cgtcatcggg tactgaaaaa
     5221 caaagcccgt gcgcctgacc gacgccatga taaggatcct gcccaaggat aaccactttc
     5281 accttatcaa aaggagtaag atcaaaggct gcaaaaatat ttttcccaga aggaaataca
     5341 cgataatgta acttttcgtc aattaaaaat tctttcaact gaacaaagta agatgactca
     5401 aactgatctt tgagcataag cttccaactc tcttgaattt ttggatttat atttgacata
     5461 aataaacact tggattttaa aataataatg ttgcaatatt ataaaaaaat tgttgttatt
     5521 tacagaaatt cttttgcgaa caaaatttaa gctaaaaatc atagtgaaaa tatgataaaa
     5581 gtaatgaaaa ctgctatcgg tttggccatt gtactcatgg tttttttcct tgtcagttct
     5641 tgtaaaagca cgcaccaatg ttccgcgtat ggtgaagtaa aaaaacatca acgtgagtcg
     5701 cgcaggtaat aacctttgtc aaattaagca taaatgtaga ttgcattttt gatattgaaa
     5761 ttattgagat gttttattca aaacatatac tgctcctgtt tcttgctctt tttatctcta
     5821 ctgaaatatt ttgtcagact gatgatctgg acattgatcg ttttgataca actctcaatc
     5881 gggttattct acgttacgaa aagtcgggcg gcttgttagt gcataccggt ggttttggag
     5941 ctatattccg gaaagctcat aatgtgaact acttcagaaa agatctttgg gaaattgaat
     6001 ttaccggaat gcagtcggaa aaacaagtta ggattaactt gtacggtacc tattatagca
     6061 atgcaaacag ttacatttac ggtaagttaa acaaagttta catgcttcat acgggtcttg
     6121 ggcagcaaca cttgctcaac agcaagccct actggggagg agtagaagta cgtttcacct
     6181 attatggagg ggcctcattg gcaatagcca agccaatcta cctttatatc atcattgatc
     6241 aaaccaactt taacttcctt gaacttgaaa gccagagata cgaccctgaa aaacatttta
     6301 ttgataatat ttacgggcgt gctccattct tagatggctt gggtaaaaca aagttttatc
     6361 cgggcattta cgggaaggcc ggacttaact ttgaatatgg cgagtataat caaatgttaa
     6421 aatcacttga attaggaatt atggctgaag gctatcccat tcctatacca atgatggctt
     6481 tcagtccgtc gcgttaccat tttattaatt tttacctcaa cgttactttt ggtaaacgtt
     6541 ttaataaata ctaatcattg atgcaacgac tttttgctgc aataaaagtt attcctgaag
     6601 ttaaacttct gagcaactat agagaattaa aaagtttgtt gcacaacgat aagatcacct
     6661 gggttggtga acaaaatatc catatcacgt taaagttttt tggcgacact cccgaaagcg
     6721 atattcctac tatcgaaaac atacttagtc aggttgccag tggtcatcac ccttttgttt
     6781 tgaatctgga gtcaatcggt atttttggca gtagttataa tcctcgggta atctggttag
     6841 gtataaaaga gccggagagc gtaaatgcgc tggcggaaga tatacttaac actttggata
     6901 aatcaggtta ccctcgtgat gatcaacact tcagaccaca tcttactatt ggacgagtaa
     6961 agatgctaaa attcaaaaag gatttccaaa atatcattaa tcggttcaaa tcaacttatt
     7021 ttcaagaagt tcaggttaac agttttgagc tgttcgaaag taagttaaca ccccaaggac
     7081 ctatctacac aaaacttcaa acattctatc ttggccgaaa tgggtaggga ataaagcgta
     7141 tggtgtgttg ttgtcttcca ccccaaactg attgatagtt gagcccataa ggattatcta
     7201 atgaacattt ttggttcaga atggcatctt atgatagtcg aaagtttggc cgtcaacaaa
     7261 aaatttgatt tgtttagcta tccgtacaaa tattttcaat tccctattat tcaaccagtt
     7321 gattgaatcc tgatctcccc ttgaagcttg cgcaagaatt actaagaatt caaaatcata
     7381 tctcttcagc catgctaaag cttgatcgtc atctaccgat gcagcatcaa aagcagcaaa
     7441 atgttgaaac ccgtgatcga gtaaccattc tatagctttc ttatcacccc ttattgcgtt
     7501 tgccaaggcg gcaagctccg gataattatt atctaacaac catctggcaa attcatgttt
     7561 accacttatg gccatctcaa gagcaaggag gatctttacc ggataatcgt atagctgtat
     7621 cacaaaaaga gtattatctg ccaaaaataa cgaaaatatt tagctgatga atcacagatt
     7681 acaaatagag cgttatcagc actaatagaa aaggcttttt attgattaat acaggttaat
     7741 aaactttgta ctccactcct atttctttaa gtttttcagc tatagcaagc aatttatctt
     7801 tgcctaccac tttcaggccg agcgttggag tttgccggtc aatagggtaa agcattacat
     7861 aacgaggttt aattaactta atgtgattaa tccattcagc aacctcagca tcggttgtgt
     7921 tatctacggc acgaccttta aattcgccct gcacaaagag ggtttgaata atagcttttt
     7981 taccaaattt ccgaagatta ctaacgatga catcgaggtt aagctctttg gatctgggtc
     8041 ggttaataag gcggaaagta ctatcgaagc ctgcatcaag ctttaagata ttgttgtcaa
     8101 cctgattgag ggcattaaaa atttccggtt tatgtatcat gcttgagttc gacaataccg
     8161 taacatcagc ttcggggaaa aacttgtctc taagttttat tgtatcgtgg atgattccag
     8221 aaaactctgg atgtatggta ggctcaccat ttcctgcaaa ggtgattgaa tcggggaaat
     8281 ttttgttcgc tttaagattc aatagcttaa cctctaactg ttcagcaact tcattacgag
     8341 ttgggagttt accaaaataa tcactttcgt cgggcgacca gccacattcg cagtatatac
     8401 aatcgaaagt gcaaatttta tgagtattag gcaacaaatt aattcctaat gagttaccta
     8461 atctgcgact tctcacaggt ccaaaaatag tttcgctgaa taaaaaaccc gccatttaat
     8521 gtatctatca aaaattaaga aatatatcag aaaacaaaaa taacgacgga atgttatgtt
     8581 aatcgaaaaa ctattacatt tttatttatg tattttcgcc acacaaattg atttatggca
     8641 aacatcaaag aaataaaaga gccaatcaac gatcatctcc ttgctttcga gaaaaaattc
     8701 gaaaaagcca tgaaaacccg tgttgccttg ctcgattaca ttacacgata cataataaga
     8761 cgtaaaggaa aacagatgcg gccaatgttt gtcttttttt cagcaggaat taccgggcaa
     8821 attaccgagt caacttatcg tgctgcctca atgattgagt tgctacatac ggccactcta
     8881 atacatgatg atgtagtgga cgactccaac atcaggcgcg gctatttcac tataaatgca
     8941 ttatggaaaa acaaaatcgc tgtgcttgta ggagattttc ttttgtcaaa aggtctttta
     9001 ttagcgcttg accataatga gtttgagctt ttgaaaatcg tctccgttgc tacacgcgaa
     9061 atgagtgaag gagagttgct acaaattgaa aaagcacata aactcgacat cgaagagggt
     9121 gtttattttg agattatacg caaaaaaaca gcctcgctca ttgcatcatg ttgtgctgca
     9181 ggagcacact cggcaggagc cgataaagag acgattaaaa aaatgtatca gataggtgag
     9241 catattggca tggcattcca aataaaagat gacctgttcg attaccaaaa aaacaaagaa
     9301 gtaggcaagc cgaacggcat tgatatcaaa gaacagaaaa tgacgctacc attgatttat
     9361 ctgcttaaca actcatcatt cttagaaaag aaaaaagtaa ttaacattgt aaaaagacat
     9421 aaccttaatg ccgaaaaggt agatcgcgtg atagaacagg taaataaaag ccagggaata
     9481 gaatatgcta ctcgcaagat gtatgaatac cgcgataaag cttttgaact gttgttggat
     9541 ttccccgagt cgcctatgcg caaaggattc gagcaattgg ttatttatac taccgaaaga
     9601 aataagtgat cgtttaagaa tatcttttaa atcactttta taaaaacaaa cttaggcaga
     9661 gtaatacttt tacttaattt cggtaccatc tacataatta tcaggtggag ccacaaagcc
     9721 aagtgaaccg tcaggatttt cggccatcag tatcattcca tgcgaggtaa tccctctcag
     9781 ctccttaggc gcaagattta ccagaataca cactttacgg ccaatcaaat ccttggcatc
     9841 ataatgttcg gctattccag aaacaacagt tcttttgtct attccggtat ctatggtaag
     9901 tttcatcagc tttttggtct tagcaacctt ttcggcttcg aggattgtgc cggtacggat
     9961 atccattttt gcaaattcat catattgaac aggctctttt agcggattgg gaacaaactt
    10021 atcattctga tttgccctac ttgtagcatt tagtttctca atctgtgcat taatggtttc
    10081 gtcttcaatt ttctcaaaaa gaagttcggg attgttaatt ctatgccccg gttttagcaa
    10141 atggatgttg ccggcctctt tccatttcat ttgtccgata tttaacattt ctgacagctt
    10201 tttcgcagtg aatggcaaaa acggcgcagc aacaatggta agattagcgc atatctgaag
    10261 tgaaatattt aaaacactct ttacacgatc aggatcagat ttaaatattt tccacggttc
    10321 gttatcggtt aaatatttat ttcctaatcg ggcgaggttc atcatctcgg caagcgcctc
    10381 tcgaaaacga aaattttcaa gactcatact tatcctttcg ggaattgcct ctatttcggc
    10441 aatcgctttc agatcatctt cattaaactg gcttgcttgc ggaacaaggg catcaaaata
    10501 tttatgagta agaactaagg ttcgattaac aaaattgcct aaaattgcta ctaattcgct
    10561 gttgttgcgt gtttggaaat ctttccacgt aaagtcgtta tcttttgttt ccggagcatt
    10621 tgcaaggagt acatatcgca acacatcctg tttcccttta aactcaacaa gatattcgtg
    10681 aagccaaaca gcccaattgt tcgaggtgga aattttctgt ccttcgaggt ttaaaaactc
    10741 aaaagccgga acgttgtcag gaacaatgta agagccttcg gctttaagca ttatcgggaa
    10801 aataatacaa tgaaacacta tattatcttt tccgataaaa tggattaatc tggtttccgg
    10861 atctttccac cacttttccc attctacacc tttttgttca gcccattcgc gcgtagcaga
    10921 tatatatcca attggcgcat caaaccacac atagagcact tttccttcgg cttcggacaa
    10981 tggcactttc acaccccagt tcagatcgcg tgttacggca cggggctgca agccctgatc
    11041 aatccacgat ttcacctgac cgtaaacgtt tgttttccaa tcaaccttgt gcccttcgag
    11101 tatccattcc ttcaaccaat cggtatattg atccagaggc aaataccaat gtttcgtttt
    11161 cctgagcacc ggctctttac cgctaagcac cgaacgaggg tttatcagat cggtagcatt
    11221 aagtgatgtg ccgcaatttt cgcactgatc gccataagcc ttttcgtaac tgcaaatagg
    11281 gcatgtgccg ataatataac gatcagaaag gaaagtctga tgagactcgt cataatattg
    11341 ctcggtatat ttttcaataa acttaccttc atcatacatc tttttaaaca tttccgaaga
    11401 agtttcatga tgaatcttat tggaagtgcg ggaatagatg tcgaaagaga taccaaattc
    11461 agcaaaagat ttctttatca gttcgtgata tttatctacg atatcctgag gcgttactcc
    11521 ctgttgccac gctttaatgg taataggcac cccatgttcg tcggagcctc cgataaaaac
    11581 cacatccttt tcatttgacc gaaggtagcg aacataaaga tctgccggaa catacacacc
    11641 agcaagatgg ccgatgtgca acgggccatt agcatagggc aaagccgatg taaccgtata
    11701 acgtttaaat tgctttttgt tttgcataaa gcctttttga ttagaaccgg caaatttaca
    11761 agaaaaccaa ttatccgata accacaaata gacgattatc tgagcaactt catttttgga
    11821 gcaaacccaa atgcttaatc acaagaataa gccccatcac tgatacttaa aagatttatc
    11881 ctttgtgaac caaaataaaa attgcgcttg acacaaacac taaaccaaga ttagtcttat
    11941 aaaatcaaga gttcagccaa aacattttac caaacattaa tttgggttga atttaagagt
    12001 ggttgaagaa aataatttac ctttcaaaat gttatttcaa tgaaaagcat aaaatgctaa
    12061 aatgagatac ggctggttat tattgatttt gctttggata aattcatttg cagtgcatgc
    12121 tcagagcaac agtgggcctg tgcgtgtgga attagaggca cgtgctcagg tgttcaacct
    12181 tgttccttgt aatgataaag gaattatggt tttttatgaa actatcaatc agcctgacca
    12241 gcaaaataaa acatggtttt ttattctcca caacaccgct ttggaaacta tttggacaag
    12301 agaaataagc attaacgttg atttcgtttt tcaatcgtca tttattgata atgaaaatct
    12361 gtttcttgtt tttcaaaaat caacaaaagc taaaactgat agtcatttct ttcaattgtt
    12421 aaatttaaac ctgcaaaacg gcgaatcggt tacttattcg atacccatgc ctgcaaattc
    12481 ggacattgtt accttcgaaa tcttttccaa ccatctgatt ttaggtttaa atcatggtaa
    12541 agagcaggca actttgttaa tcaaagatct atccacaggt gatgaatcaa cagtaaaatt
    12601 taatgatcaa tcaactttta taaaagattt aaaaatagat gccgcaacct caacgatata
    12661 tacagcgcta aacatatttc aatcgaaaag agagagttct ttttatgtta atgcatacaa
    12721 catgagcgga caactgttgc aaagtatagc attaacaccg ccccaaacta cagaaagaat
    12781 aatgaatggc cagttgcaca ttgacaacga aaagaatata tatgttatcg gcacctacaa
    12841 caataccaat ggtaaagcaa taaaggaaga tcgcacaaaa tctggagacc cgtccgaagg
    12901 attctatatg gctggcattg taggcgatag ccagaaattt ttgcacacat acagcctgct
    12961 taacttcagt aatataagac gtattctgaa taataaacaa ttagccgcgg ctgagagttt
    13021 gcttaaaaaa caaaatagac aaaacaaaga acaggggttg acttacgact ttttggtaca
    13081 taatttacaa gtaaatgatg atgagttgat tattgctgct gatgcatttt atccggaata
    13141 ccggcaggtt tcgaccatga gttacgactt ttacggccgt ccgatgccat attattacac
    13201 aatttttgaa ggttatcagt atttcaatgc ctttgtagcc ggtttcaaca agcagggtga
    13261 attgggatgg agtaatggaa taaaaatatg ggagatgaaa tcgtttcaga ttaatcaaaa
    13321 aacagaagtt tttattgacg atgatgaaat gttcatcttt tacaatcatg atggaaaaat
    13381 cgtctcaaaa gcttttcata aattcaatga cattggcaca atagaaaaca ctcaaatcgc
    13441 gaccaacttt ccaggagatc ggctaataga aaacagccag ggaaatataa aacactggta
    13501 cggcaacaac tttgtagcat acggctatca aacacttcaa aacaataccc aaggtagtag
    13561 cgagcgccga aaggtgtttt acatgaataa aatagcttta gactatcaat attaagcttt
    13621 atctcttata accttctcca tccacttaaa gtctttctcg atacactcaa gaaacttgag
    13681 ttgactagca gcccgttgat aattatgaat ctggcttttt actttaaaat atacatctcc
    13741 atttaaaaag tcagtaagaa acctcacagc cataatataa gtgagtaatt tgcaactaaa
    13801 ggcaagcagg tcaacctcaa catgtttcaa agttgcggag gttatgctaa gatagccatt
    13861 aacaaaagct tcgaaaagct ttttattaaa gtttactttg gtcaaatcgc tttcatcttc
    13921 aacagcggtg ttgcacagtg tgcgaatagc gtcgccaaaa tcatacaata cgctcccatt
    13981 cattactgta tctaaatcaa cgatagcaac tgccgaacgt cctttgtcga aaagtatatt
    14041 gttgaacttg gtgtcgttat gggtaaccct gatcgggatc aatccattgg attccaattt
    14101 atgaatccta agcagttcac cttcccgggc aaaaataaaa tccacttctt tttctacctg
    14161 tttaagtcgt ccataattat tttcttcaac agccttgtat agcgccttaa gcctcatttt
    14221 caaattatga aaatcgggaa tggtttcata taactgatta cccggaagcg ttgataaaat
    14281 agtttggaac ataccgatag ccttcccgcc ttccattgca atagccggtg ttattctttt
    14341 tattggttca tgcggaatat aggtgtagca acgccagtaa tttccaaata tatcaaggaa
    14401 aaaactctta ttatcttttg taaaaataac tgtcggcgct cttagaattg ctttggcaga
    14461 attacttagg ttactttgat taatatgact tgttactcgc gaaatattat ccatcaaagc
    14521 ttcaacttcc ttaaaaatca aatggttaat acgttgaaga aggtaatttc tatttgtatt
    14581 tgtctttaaa aaaaatgtat cgtttatatg cccttcccct atcgtttcaa ttttcagtat
    14641 cttatcctca atactgaaat tgtttataac atcaaaaaaa tcaaatgacg agggtagtgg
    14701 aatcataacg ggttaactcc ataggttttc aggatacaag ccgctgttta tcaacttgtt
    14761 gattttttct ttggccaact ctttatcttc acgataagta actccaaacc attttgcatc
    14821 agattccaaa acttgaatgt ctcctaactt tttttcgatc atccaggcaa gtggcttcgc
    14881 aatgtaatat tcagccttca agatgtcatc agcatttgtg ataaaatcag caaagccctt
    14941 tttaaaaagt tgaaagatac cagggtaaaa cccccaggcg ttcatcgaaa cgatggtatc
    15001 atcttcaatg gtactccaac cctgtttagg gtgattatat ccaatttcgt ccgatcttcg
    15061 ctccacattt gtaacctctc ttataccttt aagaatcata tcttcaccat aggagcatat
    15121 tcctcgcgat acggttccat gatcggatag tgtatattgt aactgataac ctaccataca
    15181 atgttttttt agattgttgt ttgttgtaaa atattgtaca atagactggt aggcttccat
    15241 tccataaaag tcatctgcat ttattaccat aaaaggctcg tttataacct cacttgccac
    15301 taatactgca tgtgctgtgc cccaaggttt tacacgaccg gcaggcgcca caacaccttc
    15361 gggtaaatta tcaagctcct gataaacata ctgtatgtta agcttccctt ttatcttgtc
    15421 attaaaaaaa tctctgaaat cggcttccaa ttctttacga atcaccatta ccacttttcc
    15481 aaatccagct ctcatcgcat cgaaaataga ataatcaata atcgcttcac ctgatgggcc
    15541 gattttatca atttgcttta aaccaccata gcggcttccg atccctgctg ctaagattag
    15601 taatgtaggc tttttcatta tttacatctt ttataattat caacattcat tttcgttcaa
    15661 gaccaacctc accgactaat tgaaagttaa tcttttccat gccttagtca aaataaaggc
    15721 tgataccaca gccaaccctg tgagtaggct ggccgtaaca ttatttccat aaatccaatg
    15781 tcctgtggca aacaaagcgc tataaatagc tacgctcccc agcaacatac aaagaatacc
    15841 ggttggcaca tcccacgact tgctcatctc cgggtcaata ggattccctg ataattcagc
    15901 atcttttaaa actttcttcc agcctggccc gcccggtttt atcttttcgt aaaacgaacg
    15961 taaagtggca tgtcttacgg gctttgtaag ataggtaact gttatccaag aaatagaagt
    16021 gagaaccact acccccacca actttaaaat attccaatat atttcggaca tagtgatacc
    16081 aaaaacctga acataacccg gcatttcggg tactattttc aagaagaaat tttcgatgat
    16141 aatgaataca agtgaaaata ttgtggcggc aatcatggcc gatatctccg aaaaggcatt
    16201 aattctccac caaaaccatc gaaggatata gatcaaaccg gttccggcgc caatcattaa
    16261 tatatactga aaagcttgta gtgcattttc aagaacaagt gcaagcagaa caccaaaaac
    16321 cataagtaaa acactgctga cgcgtccgat cataaccttt tgtttctcac ttgctttcgg
    16381 attaacaaac ctgttgtaaa aatcattgac caaatatgag gagccccaat ttaaatgtga
    16441 cgccactgtt gacataaatg cggcaatcag tgacgctacg accaatccta acaatcctgc
    16501 cggcaaaaaa gtgcggagca ttgccggata ggcaaaatct tctttaatat actgctctgc
    16561 cacaccaggg aaggcgctta ccattgactg gatatcaggg aagatcacta aagaggcaag
    16621 tgcaacaatg atccaaggcc atggccgcag ggcgtaatga aaaaagttaa aaagtagcgt
    16681 agctcccatt gcatgacgtt cttctttggc agagagcatc cgttgtgcta tatatccgcc
    16741 accgccgggc tcagcgcccg gataccatac tgcccaccac tgcacagcaa ttggaatgat
    16801 aaatatgtat aaaaacatat cagtccgtgt taatttggga aataggctca acttcccggc
    16861 aacattctca tgcgacaaca accctgacag tccatgaaca ttttccggca atcccttaac
    16921 aacaaacaca gcagctatga ttgctccgaa catggcaaac ccaaattgga acaaatcagt
    16981 ccataaaatt gatttcaacc ctccaagtgc gctatagaac acaacgatta ttgcagcaaa
    17041 tatcaatgcc ggcgctggtg gaacacccat catcacaccg gcaagcttga cgaaagccaa
    17101 agaaacactt gctatcacca ttacattaaa aaagaaccca agataaattg ctctgaatcc
    17161 tcttaaaaat gctgccattt tgccgctata acgtatctca taaaactcaa gatcagttaa
    17221 tacttgcgat ctattccaca gtttagcata aataaaaacc gtcaacatac ctgttaaaag
    17281 aaaagcccac caggcccaat tacctgcaac gccattttgt ctgacaatat cagtaacaag
    17341 gttgggagtg tcagccgaaa aggtagtagc taccatactt acacccaata accaccaagg
    17401 catattcctg ccggaaagaa aaaattcgtt atatcctcgg ccggcaaacc gagagctaaa
    17461 aatagcaatc actatcaaga ccacaaaaaa caaagtgata ataacccagt caatacttgt
    17521 aagaaccata ttttataatt ttccggcgaa aataatcatg ttttcaaaat aataagaaaa
    17581 acatgataga atatggattg cataatacaa tttattaaaa gggagagtga atcctttttg
    17641 aataaatatg acatctttag tgaaaataga gcaggattta attaaaaagc caatgccgtt
    17701 aaaacgacaa tttcatcatc attccctgat caattatgtt tattcaataa acaataatca
    17761 ctaaaatttg tgagaaaatt atatttttac aaaaaataat ctaactaaag tgattacacc
    17821 atccaactta cacaaaggcg acttgatcgg tttgatttcg ccctctgcaa agattgatcc
    17881 ttcgattttg tattctgcca caagattact tgaagccaaa ggatacagag tgaaacaagg
    17941 ggagcatgca ctaagtcagc attactattt tgcaggtacc gatagccaaa gagcgcacga
    18001 ttttcagcta atgttggatg atccgtcaat taagatgatc atttgtaaca gaggtggtta
    18061 tggtatggtg aagataattg accgattaga tttttcctgc tttttagata atcccaaatg
    18121 gattgccggc tatagcgata ttaccgtgtt acattgccat ctggcaatgc tttataaaac
    18181 aaaaagcatt cattcgataa tgccttatga ttttcctgcc gatggcaact cctgtttagc
    18241 tattgataaa ctcttggagg ttgccggagg taaaaagcct acctacaacc ttcctactca
    18301 tgatcttaat cttgagggat cggctaatgg tatgttggtt ggaggtaata tatcaatcct
    18361 aaccagcctt ttgggcagtc agtccgaact ggataccgag ggcagtattc tttttctcga
    18421 agaggttggt gagcctcttt atcgcctcga caggatgatg caaactttaa aacgatcggg
    18481 taaacttagc tcgctggccg gacttattgt tggaggaatg acaaaaatga acgaagaaca
    18541 agaggattat gggctcagtg cagaagaagt aatcatggat atgatcagtg atgtgaatta
    18601 tcctgtttgt tttggctttc cagcaggtca ttttcccgaa aaccatccac taattattgg
    18661 tggtgaagta agcctttcag taaacaaaaa ctcggtaaaa atcattttta aataaatcga
    18721 acttcagaat ggcagatcat aacataacag ggaacattgg tgaggagctg gcggagaaat
    18781 atctacgtag cttaggttac gaaattcttg agaaaaactg gcgttatgga aaaaacgaga
    18841 ttgacatcat tgcaagggat gatcgtttcc ttgtaattgt agaggtaaaa actcgtaaat
    18901 caatatatta tggtgaaccc gaagagtttg tcaatcgttc aaaacaacgt tcgctaatag
    18961 ctgctgccaa cggttacatc agcaaaaaaa atattgatct ggagacccgt tttgatttag
    19021 taagtgtaat tttttccgga aataagcata ctatcagaca aatcaaggat gctttttatc
    19081 ctattgtcta accttttgtt aatgatcgtg aaatgacttg aatccttcgc ccagcacctc
    19141 ataagcatca acaacagtga tgaaagcgcg cgggtctatt tcacggataa aatcctttaa
    19201 tatctgatat tcgcgccggc ttacatttgt gtaaataaca ttcctctcat tgttttgaaa
    19261 cataccacgt ccaacgaata tcgtacctcc ccggtttaaa tcgaagataa tcttctgtcg
    19321 tatctcttca tgattatcac tgattataaa catcattttc tcgtaagaga taccgcgcag
    19381 agtaatatca accacctgtc ctgttataaa aataactatc catgaataga gtggtatttt
    19441 ccaatcgcca aaagcaatca atccgaggag aacaataata gaatcaacta ttatcaaaag
    19501 ctgccctaaa ggaacgccgg taaacttatt tatcatcatt gatatcaggt cggtacctcc
    19561 cgttgtagct cttgaccgga agatcaatcc aaggccgaag ccaatcagga ctcctccaaa
    19621 aatgctcgcc agcaatatct gatcacctaa attcaaagga tcgtcctgac caataaaata
    19681 tgtcaacaca tccataaaag tggctgtaag aacaaaacca accactgttt taattccaaa
    19741 tcgcggccct aatatcttga cacctaaaag agcaagtgga atatcaattg taagcgccat
    19801 cagaccaatt ggaattcccg taggccaaaa tgagaacaca ccttcggtaa gataatggat
    19861 aacaatagaa attccgatta cacctccagg tacaactcta tacggattga taaaaagaac
    19921 atatccggcc gacattatga atgtgccaaa aaatataaga ccataaactt taaaccactc
    19981 cctgctaaat aatttttctt tcgtaacaaa ggtcataacc tactgatttt taattaataa
    20041 ataaaggcgc gcaaaaatag ttcttgcgac caaattaacc taataaccac atcgaaggta
    20101 aggatttgtt taaaaaaaat aaagaaagaa ggattgatca aatcctgatt atcgtgaaaa
    20161 attaaaacat gagatagaaa cttaaaccta ttatcttttt atttgttgac ctatggttaa
    20221 aaaacaaatt ctttcttaaa aatgagaatt ttatttttaa tgataataat tcttacggct
    20281 ttaattttaa atgcacaaaa aactatgaat tacaaatcat taacacctga cgagcaatac
    20341 gttatagtga acaaaggcac cgaaaggccc tttagcggaa aatataataa tcatcatgaa
    20401 aaaggtattt ataaatgtaa gcaatgtgga aacgctcttt acagttccga agataagttt
    20461 gattccgggt gcggatggcc aagttttgac gatgaaatac ctggcgctat aaaaaaagtt
    20521 gcggatgccg atggccagcg tactgaaatt gtatgtgcaa aatgcaatgg tcatttaggg
    20581 catgtatttg tcggagaagg ctttacgcct aaaaactctc gtcactgcgt aaattctatt
    20641 tctcttgatt tcatttccga agcaaaaaca tcaaaagcaa tttttgcctc aggttgtttt
    20701 tggggtgtgc aatatcattt caataaagaa aaaggagtta tcaagacttt agcaggttat
    20761 acaggtggtc atgtaaaaaa tccaacatac gaagaggttt gcactggaac caccggtcat
    20821 gccgaagcag tagaggtgat tttcgacact gctttaaccg attatgaaac tttgctcaaa
    20881 ctctacttcg aaacccacaa ttttacacaa ataggcgggc aagggcctga tattggcgaa
    20941 cagtaccgat cagtaatttt ttatactgat aatcaacaaa aagcgatagc agagaaattg
    21001 ataaaacatc ttgaagagaa aaactacaag gtagctacaa aattggaaaa agccactcaa
    21061 ttttatcctg ccgaagaata ccatcaagac tactacttca aaagaggagg tagtccgtac
    21121 tgccacatta aaaagaaggt tttttaagac cttttcagat tatacaatct tttgatcctg
    21181 gaaagacttc gattttactt ttttggctta tgactataag acatccagaa ttatgtcaat
    21241 aatggctttt gattgtataa cacctgtaaa aaaacatgtt gaattcctgt ggaaaaaagc
    21301 cggttaataa gtgtaaccag ataaagacaa attaaaagga tccttgtatt actaatcccg
    21361 caaaatcagg attaccctca tttattatta tattgattca tcgtgatacg agggccagcc
    21421 aatacaaatg atttaatcat atctactgct actggaatct gtcgtaacat aatctcttct
    21481 tcgcttttag tccatttacc caatacatac tctacctgaa agcctttggc aaaatctttt
    21541 ccaataccca ctcttaacct gctaaactca gtatgttcca atgtttcgat aatatgattc
    21601 aaaccattgt gcgtaccacc gcttcccttt tgcctgagcc gtaatattcc ggtatcaagg
    21661 tcaacatcat caagcacaac aagtaggttt tcgattggta tattctcttt ctctatccaa
    21721 tacttgatgg cttttccact caaattcatg tatgtagtag gtttaataag atagacatgc
    21781 cttcctttta tttttatttc cgccagatag gcatggcgct gaaccgaaaa aatagtctta
    21841 tattcctgcg ccaatgcatc aagaatgata aagccaatat tatgtcgggt atttaaatag
    21901 tcttcgccta tgtttcctaa cccggctatt aagtatttca tctctttctt attacaaaag
    21961 gcacgaagcc gtatattctg ctgcgtgcct ttaactaact aaaagattat gtttcaccag
    22021 atcaattagc tgcttcaggt gcatcaggtg catcagtagc tttagcttct gcgccttctt
    22081 cagcaacttc ttcctcttct tcaacaactg ttcttacact cttaacagcc accacaatgg
    22141 atgaaggaga atcgaggaac tcaacattat ccaatttcaa atccatgacc ttcctagaat
    22201 cattaacgtt aagattggtg atatctacca ctatttcatc tggcaaatgt tttagaaaag
    22261 ccttaatctt aagttttctg tacttgcgca ccaacctacc accttttaat acccctttag
    22321 caataccgct atagtttaca ggcacagaaa tcactactgg tttgtcctca aaaatctgca
    22381 ggaaatccac atgcaaaatt tcatcagaaa ccggatgata ttgaacatct ttcaagattg
    22441 aaagaatttt ttttccatca atctcaagtt caatgatgta actgttaggg gtaaaaatta
    22501 tcttcttaaa gcttttagca ggaactgcaa aatgtatttg ttcctcgccg ccatacaaca
    22561 cacaagggac attgccatcc ctgcgtagct ttttagcatc ttttttccct acgttctctc
    22621 ttcgagaacc gctcaatgat actgatttca taaaaaataa ttattaaaag taaataaaag
    22681 tgattatatc attgccagaa aatcaaagtg agtactgata gatttatttt tgtgtacccg
    22741 tttgattaca tcggcaaaaa tttctgcagt agaaagaaca gttatttttc ctttttgttg
    22801 cctttccagt gtatcagaca ccaaaacttc ggttaaagcc gacttttcta ttttctcttc
    22861 ggcatctcct gaaaatacgg gatgcgtaca tacagcccta acacttgagg ctcccatatc
    22921 catcatcata tttgcagctt ttgtaatagt tccggcagtg tcaatgatgt catcaaccaa
    22981 cacaatatcc ttatctttaa catcgccgat aattctaagc gaagaaactt tattagcaac
    23041 ctcacgatgt ttaaaacaga ttaccagatc agtattaaga aatttagcat aagctgcagc
    23101 gcgtttggtg ccgccggtat cgggcgaggc catagtcaaa ttaggaagat ccattttttt
    23161 tagatgtgga acaaaaatcg aggaagcata catgtggtca accggtatat ctacaaaacc
    23221 ttgaatctga tcggagtgca gatccattgt gattatacgg tcaacaccgg cagctactaa
    23281 caaattggtc atcagttttg cgccaatact tacacgaggt ttatctttac gatcttgacg
    23341 tgcaaaacca aaatagggaa tcatggctac taccctttgc gccgatgcac gttttgcagc
    23401 atcaataagc aaaagaagtt gtaaaagatt atcggccgga gcatttgtag attgaattat
    23461 aaaaacatca ttgcctctta tcgtctcttc aaaacatggt tggtactcgc catcttgaaa
    23521 atgttggatt gtaacctttc ctaatggttg cccataggcc tctgcaattt tgtcggcaag
    23581 atatttagag ctatcgcctg agaaaatatt aacatttttt gccatgagac tgaggatttt
    23641 ttggttaatc tggattgcaa aagtataaga tttatctctt ccaaaaaatt tatctgtgaa
    23701 ttcatttttc tatctcagaa aataatacct ttgcaatctc attatttctg aattaatagc
    23761 ttctgaaata ctgatttcat aattttatcg ttcgtttaaa taaatcgccc gagtggcgga
    23821 attggtagac gcgttggtct caaaaaccaa tgagattttc tcgtgccggt tcgattccgg
    23881 cctcgggtac cagataaaac cctgcaaaga tgattttgta gggtttttta ttttataaac
    23941 tgttttcact aagccggttt taccttttca gtttttcaaa tccatgattc tacttttcaa
    24001 agcaatacct attagttacc atccgttcaa aatccatatt tttgccagca cgaaaaaaaa
    24061 tagcttagcc tgatgttatt ctcacttgat acgattgacc caaaaacttc ggcacgcgca
    24121 ggaactatta ctaccgatca tggaactatc cagacgcctg ttttcatgcc ggtaggcact
    24181 ggcggcactg taaaagctat tcattaccgc gatttatatg aagatagcaa agctgagatc
    24241 attttgggta atacttatca tctttacctc cgaccaggat tgggtattct tgaacaggca
    24301 ggcggcttgc atgagtttat cggttggaaa agacccatct tgaccgatag cggcggctat
    24361 caagtttttt cattatccgc tcaacgaaaa ataaaagatg atggcgtcca tttccgctca
    24421 catattgacg gatcaaaaca tctattttca cccgaatcgg tcattgatgc tcaacgaatt
    24481 ataggagccg acataatgat ggcttttgat gaatgcaccc catggccctg ccagtatgaa
    24541 tatgctgcaa aatctttgtt attgacacac cgctggcttg accgctgcct ccaccactta
    24601 aacaatacag agccaaaata tggttatcaa caagcgctct ttcctattgt ccagggtagt
    24661 acgtacgccg atttacgcaa gcaatcagca gagtacattg caacaaaaaa tgctgcggga
    24721 aatgcaattg gcgggctctc cgttggcgaa ccggccgacc tgatgtacga aatgacggag
    24781 atggtttgca atatacttcc gcacgaccgc cctcgctacc ttatgggtgt gggtactccg
    24841 gtaaatattc tcgaaagtat tgcattaggt atagatatgt tcgactgcgt aatgcctacc
    24901 cgcaatggac gtaatgccat gctttttacc agcgagggaa ttataaatat aaaaaatgaa
    24961 aaatggaaaa acgatttctc tccaattgat cccaacggaa cctcattcgt tgatcatgca
    25021 tatacaagag cttttctgcg tcatctgttt gtttcgcagg aatacttagc gccaatgatt
    25081 gcaagccttc acaacgttac tttctatata tggcttgtaa aaacagcccg agccaaaatt
    25141 attgaaggga cattttcagt atggaaagat gctatgatac ccaaattaaa caacaggcta
    25201 taattttcaa cttcacaatg agttatttag ctctgatagc ttatgaaaat cattgataga
    25261 tatatcatcc ggaaattcct tggaactttt ctttactcca ttgcactact ctcggtggta
    25321 atcatcattt ttgatctttc cgaaaaaatt gatgactttc ttgaaaaaga ggcgccatta
    25381 aggataataa tatttgacta ttacctcaac tttatcccct tttttgtgaa catgttcagc
    25441 tatctgttca cctttatttc tgtaattttc tttacatcca aaatggcctc caacaccgag
    25501 attgtcgcta ttttaagtag tggtatcagc tttaagagat tcttatggcc ctacatcttt
    25561 tctgctacta taatagggtt attatcactg tttttggcca actttgttat ccctaatacg
    25621 aacaaaaccc tttgggagtt tgaaaaaaaa tatatcaaaa acccaatccg caacgaagat
    25681 atgaatatcc acatgcagat caatccaggg gtatttatct atgttgagag ctttaatact
    25741 tctaccaaaa ctggtcaaaa attttcgctt gagcagttcg ataataaagg caatctcgtt
    25801 tataaactca attccgatta ttttagctgg gttccggaaa caggcaaatg gaggataaaa
    25861 aactactata cccgtacaat tgataacaac aaagaggttt ttatcaaagg tcttgaaata
    25921 gatacaacct tgaatttaca tccatcagag tttactatca tcagcgacga tataaaaacg
    25981 atggactatt ttgagttaaa agctttcatt gaaaaggagc gtttgcgtgg ttcaccaaag
    26041 ataaacgagt acaacgtaga gcaatatcgc cggtatgcat ttcctttttc tacacttatt
    26101 ctcacgttaa ttggagtttc tttatcgagc aggaaggtgc gtggtggtat tggaatgcac
    26161 ttagggatag gcattgggct aactttttca tatatacttt ttatgcaggt aagccaggtg
    26221 tttgctgctg taggaggaat ctcgccaatg cttgcagtat ggatacctaa tattatttac
    26281 ggattgttaa gtatctacat gttaaagata gcgccgaaat gatttataat cgctaaacct
    26341 tactatttct ccgatttaac ttttttcaaa ttcaataatt gatttctctc atccaaaaga
    26401 ttatcaattg cctgttcagt taattcaggt ctttttaaca tttcatcaat cagccttatc
    26461 cgttcatcaa catctatttt tttattcttg tccgatggtt gatatttaaa ctcttcatcc
    26521 ttatgtaata aatcatcagt atggatatca aaatcaatat tcaaactatc tttaacagct
    26581 gatataacat cagttatcca tttattaaaa gaaaatattc ccatgccttt aaggaattca
    26641 ggaaaaggtc gctcactgga atcaccatcg ttgccgataa taaagtcaat gctttgcttt
    26701 attattggat cgaggttttc gtattgactt ttgtcaacat cggccataat ccttttcaac
    26761 aaatcaatga gttcgtcgag agaatattta tatggtccac tttcgtcttc cattattgga
    26821 gttaattaaa atttacataa aagtaacgat aaaattaaac cctaattatt caacaaccga
    26881 cctttttgga ttaaactatt gattaattca ggttaagaca aatgtggcta ttatttatgc
    26941 cagcataatt tcggtaaaat atttataaaa atatggaatg gtttctattc ctttataaaa
    27001 gttgaatagt gggtaattct catttggcga gtgaatagca tccgattcca gtccgaaacc
    27061 cattagtatg gattttaatc caagtatctt ttcaaatgta gaaatgattg ggatgcttcc
    27121 tccactgcgt accggtatag gcttttttcc gaaagtggct gtataagcct tttctgctgc
    27181 ctgataagcg tgcatgtcag taggagaaac ataaggatag ccgccgtgca gctcggtaac
    27241 ttttacttta acggattttg gcgccagact ttcaaaatag ttagcaaaaa gctgagctat
    27301 tttcttatta tcctgattgg gcacaaggcg catagatatt tttgcatatg cttttgatgg
    27361 tattactgtc ttggcgcctt cgccgatata gcctccccat attccgttca catccaacga
    27421 tgggcggata cctgtccgtt ccattgtcga aaagccggct tctccctctt cttcttcaat
    27481 atcaagtgat tttttatact catccaggtc aaatggcgct ttagccatct cggcacgttc
    27541 ggctggcata acctcaatca cgtcatcata aaatcccggt atagtaattc taaaattttc
    27601 atcctgcagt tgggctatca ttcttgataa aacattgata ggattggcaa ccgcacctcc
    27661 ataaaggcca gaatgtaaat cgcgattagg accagttact tcaacctcca tgtatgcaag
    27721 acctcgcaac ccggtagtta ttgatggaat atccgtagaa atcatcgttg tatcagagac
    27781 aaggataata tcagccttta ataattcctt gtgcttttta cagaaatttc caaggcttgg
    27841 cgaaccgatc tcctcttcgc cctcaatcat aaatttcaca ttacaaggca acgtgtttgt
    27901 tttaaccatc agctcaaacg cttttgcatg cataaaggcc tgccctttgt catcatcagc
    27961 gccgcgagca aatatcttac catctctaat ttctggtttg aaaggatcag attcccacgc
    28021 ctcaataggg tcaaccggca tgacatcata atggccataa accaacacag taggcttatt
    28081 gggatcaaca attttttcgc cataaacaac cggattaccc tcgctgggca tcacttctgc
    28141 cctatcggcg cctgcttcaa taataaactt cttccaaagc tcggcagtac gaagcatatc
    28201 atccttatga tcctgaatgg agctaataga gggtatcctg ataagctcaa acaactcgga
    28261 taaaaagcga tccttgttcg catcaatata cttttttaca tgttccattg aaaataattt
    28321 gtattgataa ataatgaatg tttttgattt tatctcaaaa gatggattaa tgataatttt
    28381 gcattgcaat cacaaacttt ttggcaaaaa tataatttta cttttaaaat gaaaaaaatc
    28441 agaattgcta tcaatggatt tggtcgtatt gggagaacct tttgccgaca gatgcaatat
    28501 aacaaaggct tagagcttgt tgcaatcaac gatcttgccg attcttacac tttgtgccat
    28561 ttgctcaaat acgattctgt acatcgtgct tttccaggca atgttacttg tgatccaagt
    28621 catctcatta tcaatggtca taaagtttcg atacttaata tagctcagcc ggcgcttatt
    28681 ccatggaaag agtatgatat tgatgttgtt gtggaatcaa ccggtaactt tactaaaaaa
    28741 gaagatgccg agcaacatct ggcagccggc gcacggaaag ttgtcatttc agcgcctgca
    28801 aaaggcgaaa atgatgatat ccaatatgtt gtattaggtg ttaatgatca tcttataaac
    28861 tgggatgtac ctattatcag caacgcctca tgtaccacta ataatgttgc tcctctgata
    28921 atgatccttg atgagaactt cggtattgaa gagggtttta taactaccgt acattcatat
    28981 actcgcgatc agcatttact tgatgctccg cacaaagacc tgaggcgcgg cagggcggca
    29041 gcctattcaa tagttccaac aaccaccgga gcagctaaag cttccaccaa aatatttcca
    29101 cacctcacct ccaaacttgg cggcgcaggt atcagagtgc ctgtacccga cggatcactt
    29161 accgatttca cttgtaccgt aagaaagtca acaactataa acgaaataaa tcagcatttc
    29221 aaacaagctg ccgacggaaa gttaaagggc attttacaat ataccgaaga cccaatcgta
    29281 agcactgacg ttattggcaa cccttattca gcagtatttg atgcacaact tacagcagta
    29341 cttgggcaaa agaacaggtt agtgaaagta gtagcctggt acgataacga aatagggtat
    29401 tcgtcaaggc ttgtagagtt aattgaaaaa tttgccggaa agtaactttt aatcaaaaca
    29461 ggatgtaaat atttaattta cagtaacaat ttaatttagc ttttaattcc tgattatcct
    29521 ttttccaaaa cttgatcatt actgctgttt ttatttcaga atagtaaaat ttgcttgcac
    29581 ttttttagat tttatccgat atgaatggtc gctaaagagt aattacttta cttttgttgt
    29641 gtttttttat gagttataat gaatgtattt aatacattga gaaggtatga agccagaaat
    29701 ttcaaaagaa tttatcaatc tcgaaaagct ctttcaagat aaaaacccca agttataccg
    29761 gctgattccc acttttgtat ttaaatattt acgccgggtt attcatcagg acgagattaa
    29821 tgcaaattta tatgagtttc gcgatttgca cggaattgat tttatcaagg ccgttcttaa
    29881 ccgttttggt gtgataatcc actctaaaga cttgaaaaac ctacctgcca ccggaagata
    29941 tatcattgtt tctaatcatc ctcttggcgg gcttgatggc atggcgttga tgcaagaaat
    30001 cggaaaagtt agaactgact ttctatttcc ggtaaacgat ctgcttatga accttccaaa
    30061 tctgaaacag ctatttatcc caataaacaa acacggaagc aacaccgaaa atattcaact
    30121 ttttaatgat accttcgcct cagatattct gctgctatat tttccagcag ggttagtttc
    30181 gcgaaaacaa ggaaaagaga taattgatct tgaatggaaa aaaacattcc tgtcaaaagc
    30241 tattcaatat caacgtgata ttataccagt acacatcagc ggacaaaata caaatttctt
    30301 ttacaacctt gctaactggc gtaaaaagtt aggtatcaag gcaaatatag agatgctcta
    30361 tttagttgat gaaatgttca aacaaaagga taaaacaatt accattactt ttggcacccc
    30421 aatcccaatt aaagtttttg acaaaagata ttcaaataac caatgggcta aattacttaa
    30481 agaacatgtt tataaactgg ggagcggcga tgagagtgtt tttaaataca ttactaattg
    30541 attttaagct tgataaaaat tgatacatgg aaataattat tccacaaccc gataaaagtt
    30601 tgttgttaaa agagcttact ccagagcgat ttgttaggca caccaacttc ggaaaaaatg
    30661 agatttacat ttttaactcc aacaattcac caaatttaat gcaggaagtt ggcaggttac
    30721 gtgagctatc atttcgcatg gcaggaggcg gtaccggtaa gagtgttgac attgaccaat
    30781 acgatttagg cagtaagcct tttgagcaac tcattgtttg gaatcctgtt aatcaggaaa
    30841 ttgttggcgg atatagattt gtactcatga aaaagcttgg tgttgattct tctggaaaag
    30901 ttaaaacacc tacttcaaaa ctgttttatt taagtcaaaa gtttaaaagc gactatctgc
    30961 catttactat tgaactgggg cgttcgttcg ttcagcccga ttatcaaccc tccaagaatc
    31021 ccagaatagg aatatattcg cttgataacc tgtgggatgg attaggtgca attattaagt
    31081 taaacccgga agtgaaatat tatttcggta aaataaccat gtatccaaaa acagatctac
    31141 ttgcacgtga agctgtaatg tatttcttgg gtaaattttt tcctgacaaa gagaaacttg
    31201 cgtacccaat taatgcagta aaccttcaaa ccgatgtagc attttttgaa aatttattca
    31261 ataaggatag ttacgccgaa aactataaaa ttttgcagca attcgtcaga aaacaatacg
    31321 caaatatacc tccacttgtc aatgcctata tgaacctctc cttaactatg aaaacatttg
    31381 gaacaacaat aaacagcaca tttggcaatg ttgaagagac cggcatcatg ataacaattt
    31441 ccgatattca tcctgaaaaa aaagaacgtc accttactgg catgtgatta ccttgaaaaa
    31501 atggagatta aaaacgcaac ttttatcatc agcaataccg actacagtaa atgccctact
    31561 cctaccctcc ctgaatatgc atttattgga cgttccaatg tcggcaaatc gtcgttgatc
    31621 aacatgcttt gcagtcgtaa agggttggcc agaatttcgg ttcagccggg caaaacacaa
    31681 actatcaatc acttcttgat taacgaaaca tggtatcttg ttgatctgcc tggatatggt
    31741 tatgcaaaaa tttccaagac tacaagagaa caatggaaaa cgatgataaa taattatctc
    31801 acaaaaagag agaatttagt atatactttt cttttgattg atattcgtat ttcgccacaa
    31861 aacaatgatc tagatatgat caacaacttt ggaagatggc agttgccatt tgctattgtt
    31921 tttactaaag aggataaact aaaacctact gaggctaaag caaatattga gcgttaccaa
    31981 atcgaattaa caaaatcgtg ggaagagctg cctccgctct ttattacctc ttcagtcagc
    32041 ggcgccggaa aggacgaaat attaaatttt atctccgaaa acaacgacta tttcttcaaa
    32101 tggaaaaaaa tgccttttac aaaaaggtaa tcataaaccc taaccaatca atagaaggtc
    32161 attttgggct gattacaaaa cctaacagct aaaaagggat tattcaaatt cgataagtaa
    32221 agcagatttt ggcaccatct ccccctcttt cacatacact ttctttattt tagcatcaaa
    32281 cggcgcatag ataatatttt tcatcttcat tgcttcaaga atcagaagct tgtcattacg
    32341 ctccaccttt gtgccttttt taacaaaaac cttacgaatg gtaccagaga taaacgcttt
    32401 caacttactt gggtcatccg gcacgtaagg tttacgtttt tcaaatttct ttgttaaaag
    32461 tgtccggtat ttgacgtcat caagtattaa tgttctgtat tttgggttag tttctttttc
    32521 catcggcttt ttttaatgta atttcaagat tataaaagtt gttgcttcct tttccttaca
    32581 aagcttttaa aaaggtggta tcccgtgttt tttatgtggc gggatttcgg ttttattatt
    32641 agaaatttct aatgcatgag caaggaaaat acgagtttca gccggttcta tcacggcatc
    32701 tatataacca taagccgctg cgacataagg attagcaaat ttcttcttat actcttttat
    32761 cttttgtctt ctcatctctt caggattttc ggcagaattt atttcctttc tgaaaataat
    32821 gtttgccgct ccttcaggcc ccattacagc aatttcggct gttggccatg caaatacaaa
    32881 gtcggcttta agatggtgcg aacacattgc tatgtaacca ccgccatagg ctttacgcat
    32941 aataagagta atcttaggta cggtagcctc cgaataggca taaagtacct ttgcgccatg
    33001 acgaataacg cctgcatgtt cctgttctat accgggtaaa tatcccggca agtcaacaag
    33061 tgtgcacagt gggatattaa aagcattgca atatcttata aaccgcgccg ctttatctga
    33121 tgaatcaacg tcaagaacac cggctaacac cattggctga ttggcaacaa aacctacagt
    33181 ttgcccttcg agcctggcaa aaccaattac aatgttcgca gcataaagct cctgaatttc
    33241 gaaaaactcg gaatcatcta caatagctct tatcacatct ctgacatcat acggtgtctt
    33301 aggatcggaa gggataatct cttcaatttt atatttacga gtttttgggt tcttcttagg
    33361 aaaagtgtca gccttcttaa tgttattcca cggaataaaa cttactaatt gttttatctg
    33421 gtcaaaacac tcttgctcgc tttcagcaaa aaagtgtgca ttaccggtta tttcggcatg
    33481 aacccttgcg ccgccaagct cctccatcga aatttcctcg ccaagcactg tcttaatgac
    33541 ttcagggcca gtgataaaca ttttagaaat cttatccacc acaaacacaa aatcagttaa
    33601 tgccggcgaa tagaccgccc caccagcaca aggccctaaa ataacggaga tttgaggaat
    33661 tacccctgaa gcctgtgtgt tacgataaaa aatatcgcca taaccggcca atgagttaac
    33721 accctcttga atacgtgcgc caccggaatc gtttattccg ataataggaa cttttagttt
    33781 catggcatga tccatgattt tagtgatttt tttggcatgc ataaaaccta acgagccgcc
    33841 ggcaactgta aaatcctggg cataaataca taccggatac ccgttgatag tacccgttcc
    33901 tgttacaaca ccatctccag gcaagtactt cttatccata tcgaaatcct tacctgcatg
    33961 ctcgacaaaa agatcgtact catgaaaaga ttttggatca agtaaagcta taattctctc
    34021 acgagcagta agcttacctg ttgccttttg cttttcaatt gcttgagtac caccaccctg
    34081 aagcgcatcg cgcatacggc ggcgaagatc agttgattta gtttttattg acatagtatc
    34141 tgttttatat catttttcaa ttattaaaca aacaacctat ccaattggca aaattaacat
    34201 tattaaaata cctttttgca caacagcaaa agtaactttc tgtacaaact gaacaaattc
    34261 gatgcaggct aataaaaccg gtattagtca acagaaagca ttcttaggcc taagaccaac
    34321 ccttttttag tattgccaaa atccaaaatc aaggctgaaa tcaatttaaa atttttagct
    34381 gcaaaaaaaa ctgttaaaaa aaaactgttt accttgtcag atactacttt ggttaaatca
    34441 tgcttataaa ataatttcgc aaaatctaca ttctttagag aagtatattc aaaatgcttt
    34501 aatgatgaaa aaaatatttt tttatctctc gctttcatta atgatactca acatttcttg
    34561 tttatcaaaa aacagaaaga ccgcttattt acagtcgaaa gatactattt ccggcaaagt
    34621 tatcgcagtg cacgatggtg atacttacga tttactggtt cagggaaatg aaaaattaag
    34681 tgtgagaatg gagggaattg atgcccctga acgaggtatg ccattctatc aagtctcgaa
    34741 gaggcatctt tcaagtctct gttccggcaa acaggtaacc ttaaaaataa caggaacgga
    34801 caattatggc agggctttag gttttacata ccttgaagac ggaagggagc ttggacagga
    34861 aatgattaaa gcaggattgg cttggcacta caaaaagtat aattccgaca ctgcgcttgc
    34921 caacttcgaa aaagaggcga gaagattaaa aaagggatta tggttggata aaaatccaaa
    34981 agccccatgg gaagtaagaa gactaagaag acaaggtgtg tctgcaaaag attcatttaa
    35041 tattagccca cattaaccaa taaaaaaata ttgggcagaa atcttgattt tatgatgtta
    35101 ttcctgattt aattccgact tctgatattt agtaattttt tgaaaatatt acttgtaata
    35161 tactgaaaat cttgaaggac aaggtggtta aacctatttc gaaagaagcg ttcaaatcat
    35221 cgaaaaacaa gcagcaatta aagcaggtat aaagaagaaa gtcggttcac atcttcttcg
    35281 acatagtttt gttactcatt tactcgaaaa cggcgccgat atacgttata ttcaacaact
    35341 tctggggcat cattccataa aaaccactga aatttacacc cacatcatca atgtttccat
    35401 atccaaaatg caaagcccct tggatatgct ctaaatcaaa actatctgtt caaaaatcta
    35461 aaaagtgtcg ggatttttcg tctttttaaa aaaactctat aaacaccatt aaacttcaaa
    35521 tcaacagcat tttcaggcta cctgtaattt tccgaaattg ttcttttcga gcctttccaa
    35581 cacatagctt ttagtgattt taagctcttt agttaaacta tcagaaggga gattaaacat
    35641 atcctcatta agtatagtct ccataattgt tctcaatcct cttgctccta atttaaactc
    35701 aatggccttt tcaacaattg tcgtcaatgc tccatcggtg aacgacaatt ctatgttatc
    35761 catttcgaaa agccttttaa actgtttcac caatgcattt ttc
//
LOCUS       DAOOAI010000009        34284 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_1642_length_34284_cov_5.537906, whole genome shotgun sequence.
ACCESSION   DAOOAI010000009 DAOOAI010000000
VERSION     DAOOAI010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 34284)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34284)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34284
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1642_length_34284_cov_5.537906"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..830)
                     /locus_tag="PK345_01400"
     CDS             complement(<1..830)
                     /locus_tag="PK345_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33709.1"
                     /translation="MSPAFACSGAEISLNNCQTGIEYYLYFQSFPDKQTPDWIKLTEH
                     PVVCNEDSAINFGVWADAGVYHILGVNPITNCTSWMENSTTILPNPTAFIVQPQGFAD
                     CEPVVIYLEHSEAQVTYYLWRKDDQNNMHLMDSIKGTGGAVHFKTQTGAGTYSVKAKW
                     SHQNTDCWTDMVGAVIIGAPPVSYTLMPATASCPPVSFYLTNSQDSVTYELWSETEGL
                     VDTIQGSGGAIYFKEVNNPGKYKVKARNQLGCEKLMSGIREVLPQPNNFIVTLTNGFI
                     "
     gene            complement(864..6020)
                     /locus_tag="PK345_01405"
     CDS             complement(864..6020)
                     /locus_tag="PK345_01405"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPX33710.1"
                     /translation="MKNFLQLIITFCTLIILTLDIFAQNQPVFTSNPPNFRYCDGEQG
                     LQLGVTNTVAGTTYHLQQKNGSWVTKDSKKGDGKTVWFDGVWKIGTYRYMVEDPFYTS
                     NERYVRKDNYPLDTFKITGGGGYCAGITDSIEIGLNGSEKSVNYFLYRNGSLLNNTNE
                     PGTGQPISFGYFSIPGTYTVRGKRFNCSSYMKDSVSITVFSNPTANFSASPQNQCAND
                     PVVFTNQSKANQAEAAIVSWLWDFGDPNSGSRNSSTLQNPIHVFEAWGNGSQTFDVKL
                     LVTDSNGCEAEIIKEVTVQQKPDATLSASGDGIKTTMVNDTTTFAFCGSVTFATFTFT
                     NQSTTPSINTLYHIDWGDDSPAFNSPEFNEPIYHEYSGLGFKTLTYTVSQENGCNHIK
                     KYYIFIGSTPSGGIGNPGNASGNVPFYLEFPIAEETYDNTLGTTYVFDFGDGTDPVYF
                     TQSTLPADKKIAHYYTKCSDEWNYCLFPWEGRYTFKASCVASNPCDNAYSIACPIYVS
                     CPVIPGFGVGLGWGAGQGEIMACNPVTFTNETIPGFYITGNGTTFTQNTVYSWDFGDP
                     ESGENNFSSDPNPTHTFTNSCESYTVTLIAWTGENSINNSGIDTIQKLVYIQEMPTAN
                     FDMDFTSNCVPKEVEMNNTSDIGCFGVPEYLWTVQPENDWQIITPEHEGDNTYINPVF
                     QFNVSGTYSVTLTITNSCGSDTITKSILVCQRPEVEIKGDSIFLCVGKNFTIQPDSVS
                     YNDNFSPITQYQWTSDPLGPIFNTNNPAYPTITFNDVGIYNITVVATNECGQSEPATL
                     QFDVSPGIENDSIRYYGNMQLCANMKLSEVLTGTNENSTPPLTGGDGTYTYTWQIDEG
                     SGWTEINDENSPDLDYDSTLTVNPTKFRRIVKSAGCESVSNELSFTVRSAITNNSIQP
                     TVQHICSGETPGTLMGSDPAGGGGTFEYLWEQSTDCSDPESWGPANGEPNNKTFSPPE
                     LFVTTCYRRIVYSVEGLCEDISEIVTVNVYGDIENDSIYHPDPVICEGEPAGTIYGSD
                     PSQSGHNSSWQYTWQEAVAPNWNWIDVPDVNTKDYLLPQLNHTTRFRRIAKAPAPVPV
                     NCNNNESNVIEFTVNPKPIVNAGDDINAFNGKQVTLNGTASGGSGNYTYWWEPKDLIS
                     SGQGTLNPTTVNLYPPPTNFIVFTLTVTDSITGCSSTDNMTVYISGGSLICSVTADKN
                     HICIGESVTLNGSASGGSGSYTYLWSLPEGGSSSGQQIMHTPTNSGSNTYLLEVDDGA
                     TTTQCEFIVQVDDLPFVINNSSVIKICSGEQLNYSPEANIPEATFNWYSVPNPYIYGN
                     SAGVGDINDALFNSSNEVQSITYFIQPTGPEPTFCSGTDFELTVLVNPTAQIVNSPTS
                     QTVVSGQSSAEVIFTSNVSGATFQWSSEDNCPEMLSYSTPLNGDVLPSQIIEIDSIGP
                     ETCIITFYITPYFDGCQGITYQYNIYVQKQPIQFTVTGGGVICQGGSLNVYLNGSQTG
                     VNYQLYRNGTIPVQPPKAGNGNPIEWSNITKQGIYTVVGINQSNMVQNQMIGQVAVIV
                     NPLPSIFVIVPSGNQCAPVTPLLNGSQAGVRYQLIKDGQSIVDEVDGTGEIGFLQFKE
                     QTEVGIYTINAVNLSSLCERMMDGELIIKPLPLEFPIYSSGILCEGSTICIEGSEIGV
                     NYQLWKNNNPFGAIITGTGDAICFEEQTEPGVYRVYAKNINSLCEIVFEQ"
     gene            complement(6274..7914)
                     /locus_tag="PK345_01410"
     CDS             complement(6274..7914)
                     /locus_tag="PK345_01410"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: protein motif:HMM:NF010588.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HPX33711.1"
                     /translation="MKIALTQLNYYVGDFEKNISKIKHSIQSAKKQKVDLIVFAEMAV
                     CGFPPYDLLHYDSFVGKCQQAIADIAVECTEIAAIIGAPYVNNDEFGKRLYNSALLLQ
                     NGSIKEVRHKTLLASNNSKDEHRYFEPNKKWGTISFKGEKIALTIGEDLWNIDNYASG
                     PLDILAEESPTIIINIAATPFHYDLWKERTEILTNYVLKYKLPLIYVNHVGGQGGLLF
                     DGGSLALDKTGKRLYELKFFEEDFTIIDTKVTTTTTSPKQIDNYPPEYLKIGLIHDSL
                     IMGIKDFYGKQGFTKAILGLSGGLDSAVVLLLAIKALGRENVMSVLMPSPYSSDHSLT
                     DAIHLLKNTKSPYKIINISEAYNLFRQTLEPVFGYLPTGATDENLQARIRGVYLMSMA
                     NNSGYLLLNNSNKSEAAVGYGTLYGDMNGGLSVIGDIYKTEVFELARYINYYKEVIPE
                     NIIKKPPSAELRPNQKDSDSLPDYDVLDKILYNHIEKHKSYQELIEMGFDKELITKTL
                     RLVSISEYKRHQTPPILRVSPKAFGMGRKMPIVTRYLE"
     gene            complement(7922..8614)
                     /locus_tag="PK345_01415"
     CDS             complement(7922..8614)
                     /locus_tag="PK345_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPX33712.1"
                     /translation="MKRIYLTTLLVLLISSSAVFAQMKPFKFGLKISPNVGWLSTSSN
                     GYEKDGGGIGYNWGLIGDFTLADNYFLSTGFAVNNLHGKLKYQDSRMVEGASEKVTGS
                     LHRKYSISYVDIPIMLKMCTNRFDNFRYFGQFGPTLGIKIKADAKDEFTTTAKKYFTE
                     ENIKKEINSIRGGLILGAGAEYYVDQSTCFFVGLNYSNGFTNVLKDRNPVTDIKPKAT
                     LNYFEITFGMIF"
     gene            8729..9340
                     /locus_tag="PK345_01420"
     CDS             8729..9340
                     /locus_tag="PK345_01420"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPX33713.1"
                     /translation="MKKILFVLLLTLSASLAFGQFTLGPKIGYTTSKLSTELEDIHSS
                     IKEDFDFGLFMRIGRKVHLQPEIIWTSKGAHLERTENSNLKAINQEIKLKTIDIPLHL
                     GFRLVNFGVGNIRFLFGPNASIVVDKTVSIQEKEEYINPIKTADIEDLIWAVNVGGGI
                     DLFMFTIDVKAQFGINDIVEQADNIDFHAKTNLFIVSLGWKIF"
     gene            9445..10458
                     /locus_tag="PK345_01425"
     CDS             9445..10458
                     /locus_tag="PK345_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33714.1"
                     /translation="MRNPNKLLLFSVFILVVIIISACTGRKTRDLHVNLKGVEIEPIK
                     IKRYEKALFNIPPEVLKPELKKISTVYPVFLDYDLDDTLIIIQLHRFITEPVNRELYQ
                     FTTDRYPELNFLENDFQLAFRYFKYYYPDKPLPNITTYVSGLLYELPIQFFGNEMVIA
                     LDMYLGDDVEHYRRFRFPLYKIERMNSDYIVRDGIYDFYYLHFLKKPGSNMLEKMISN
                     GKHLYFVDAMLPDVPDYIKIGYPPEKLEWCIANESNIWAFIIQNELLYSGEAGTLRKF
                     FADGPFTNQFGQQSPARVGEWIGWLIVRSYMNKNREISLNELLDNDDFQMIFNNSGYR
                     PAK"
     gene            complement(10577..11161)
                     /locus_tag="PK345_01430"
     CDS             complement(10577..11161)
                     /locus_tag="PK345_01430"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HPX33715.1"
                     /translation="MVDWLINVDRNLFLILNGLNNGFFDLIMFWVSDKLIWIPLYLFF
                     LFLFIKKYKINTLWILLAVAILITCSDQLSVLIKNLVQRFRPCHDPILQMQVHTVYGK
                     CGGKYSFPSSHATNFFALVFFIIPFLKSYWKFFPAALILWVSTICYSRIYLGVHYPGD
                     VLAGSLLGAFLGWIFAKVFLNILPKINQFINKPD"
     gene            complement(11184..12173)
                     /gene="obgE"
                     /locus_tag="PK345_01435"
     CDS             complement(11184..12173)
                     /gene="obgE"
                     /locus_tag="PK345_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HPX33716.1"
                     /translation="MSSSNFVDYVKIFCRSGHGGKGSAHFHRSRLNPRGGPDGGDGGR
                     GGHIILRGNQQLWTLLHLKYTKHVRAGRGGDGGSQLKSGASGKDVILEVPLGTVARNA
                     DTGEIEFEITQQDEEKVLLYGGRGGLGNDHFKSATNQAPRYAQPGEAGKEEWKILELK
                     LLADVGLVGFPNAGKSTLLAAISAAKPEIADYPFTTLKPNLGMVGYRDHQSFIMADIP
                     GIIQGAHEGKGLGLRFLRHIERNSILLFVIPADSKNIKKEYKILLNELKQYNPELLDK
                     SHILAISKSDLLDEELIDEIKKNLPPMPYVFISSITQKGLMKLKDMIWKELNS"
     gene            complement(12173..12724)
                     /gene="hpt"
                     /locus_tag="PK345_01440"
     CDS             complement(12173..12724)
                     /gene="hpt"
                     /locus_tag="PK345_01440"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007478471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HPX33717.1"
                     /translation="MSQNLPTINVNDKSFELFISSETIQKAVERLADRISVDLDGKNP
                     LFLVVLNGAFIFAADLLKRIKIDCQVSFVKLSSYSGTKTTNIVRELIGLDEILHGRTI
                     VIVEDIIDTGLTMAEAINKLYKLEAAEVYLATLLFKPKAFQKDFKIDYIGIEIPNDFI
                     VGYGLDYDGFGRNYPDIYKIIDK"
     gene            complement(12736..12972)
                     /locus_tag="PK345_01445"
     CDS             complement(12736..12972)
                     /locus_tag="PK345_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33718.1"
                     /translation="MFVRITIALLKKEGVTTASASTSSNGIPESAMFIIAPLSRKGFR
                     KTVVFSFFDLDHNKIKDNLYIFLKPAYIYQSIFA"
     gene            13206..13709
                     /gene="purE"
                     /locus_tag="PK345_01450"
     CDS             13206..13709
                     /gene="purE"
                     /locus_tag="PK345_01450"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005781966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HPX33719.1"
                     /translation="MRPKVSIIMGSSSDLPVMEAAAKLLNEFRIPFEINALSAHRTPE
                     EVESFARSAYDRGVRVIIAGAGMAAHLPGVIAAMTAIPVIGVPVKSSLEGLDALLSIV
                     QMPTGIPVATVAINGAGNAAILAAQILSISDKELYNRLVEYKEKLKDKVIKANDDLSN
                     IKYEFKV"
     gene            13733..14386
                     /gene="pyrE"
                     /locus_tag="PK345_01455"
     CDS             13733..14386
                     /gene="pyrE"
                     /locus_tag="PK345_01455"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HPX33720.1"
                     /translation="MIYKEEIAFKMAELLLKIKAVKINVDNPFTWASGLRSPIYCDNR
                     ITLSYHTIRTFIRQQFSAIIKEEFGNINLIAGVATAGIPHGVLVAENLGLPFAYVRSS
                     KKDHGLGNEIEGIIETGQSVIMIEDLISTGGSSLNAVNVVKEKGGMVKGLLAIFSYGF
                     QSAEEKFVAADCKMFTLTDYPSLLEFALENDYIRKTDLPILKKWREDPENWYLEIKN"
     gene            14394..14810
                     /locus_tag="PK345_01460"
     CDS             14394..14810
                     /locus_tag="PK345_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33721.1"
                     /translation="MNFIADNISLDVSKESIDNFFKDINALKLIMPAGVEQWQTEGDK
                     CSFFIKNLGNLKMKRSFDIPVSEYAYRSTADSKVDFTLLFHFNENGQNKSTGYFELQA
                     QVNPFMKMMLERPVTNFVNLLTQNLHNHFKNKCFSF"
     gene            14847..15920
                     /gene="lpxK"
                     /locus_tag="PK345_01465"
     CDS             14847..15920
                     /gene="lpxK"
                     /locus_tag="PK345_01465"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: protein motif:HMM:NF014645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HPX33722.1"
                     /translation="MNILRFLLYPFGLIYGILLMLRNKMYDWGIFKATSFDIPIISVG
                     NLSVGGTGKTPHVEYLIRLLSDEYKIAVLSRGYKRKTKGFKIADDNSTVADIGDEALQ
                     INKKFKNILVAVDENRVNGIRKIKKQYPNIEVIILDDAFQHRSVKPGLSILLTDFHKL
                     YTSDYILPVGKLREFRAGASRADIIIVTKTMRPLSPILARDLNDTIKPLSHQQLFYSY
                     INYLPLRPTTLTDKNQLAHKISSVLIFAGIENIYPLLDHVSRMVNYMEYLKFSDHHLY
                     SERDLNLIREKFDNLLGKDKIIITTEKDIVRLICPQVPAQLVDVPLFYLPIEIKIHNE
                     YSKEFNNKIKDYVRKNRKDTTIH"
     gene            15883..16695
                     /locus_tag="PK345_01470"
     CDS             15883..16695
                     /locus_tag="PK345_01470"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013548688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPX33723.1"
                     /translation="MYEKIEKTRLFIENMIGIKPEIGIVLGTGLGNFTNKMEIIKSIP
                     YNTIPNFPLSTVQGHRGQLILGKIGGKICIALQGRFHYYEGYSMQEVTFPIRVIARLG
                     IKYLFLSNASGGTNPDYEVGDIMIIEDHINLTGQNPLIGPNDDRLGPRFPDMSEVYDK
                     KLIALAKSIATDYKITVQCGVYAAVTGPNYETPAEYRYIRKIGADAVGMSTVPEAIVA
                     RHMNLPCFAISVISDLGVEGKIEKITHEEVIDAASLAEPLMTKLIKKMIQEL"
     gene            16828..18108
                     /gene="glyA"
                     /locus_tag="PK345_01475"
     CDS             16828..18108
                     /gene="glyA"
                     /locus_tag="PK345_01475"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009127490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HPX33724.1"
                     /translation="MKRDVQVFDIIKRERERQMHGIELIASENFVSDQVLEAMGSVMT
                     NKYAEGYPGKRYYGGCEMVDLTEQLAIDRACELFNAEYANVQPHSGAQANMAVFLACL
                     KPGDTFMGLDLSHGGHLSHGSPVNSSGILYNPIAYGVSKETGIIDYDQMEKTAKEHKP
                     KLIVAGASAYSRDWDFARMRAIADEVGALLMADIAHPAGLIARGVLNDPLPHCHFITT
                     TTHKTLRGPRGGMILMGKDFENPWGLTTKKGEVKMMSDLINSAVFPGIQGGPLEHVIA
                     AKAVAYGEALSDEYMEYIVQVKKNAAVMAKAFIEKGYHVISGGTDNHLLLIDLRTKFP
                     DITGRKVENTLVQADITVNKNMVPFDSRSPFQTSGLRLGTPAITTRGLKEEHMQPIVD
                     YIDKVISNIDNESLISEVKKEVNKMMNKFPLFAY"
     gene            18238..19206
                     /locus_tag="PK345_01480"
     CDS             18238..19206
                     /locus_tag="PK345_01480"
                     /inference="COORDINATES: protein motif:HMM:NF022720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3078 domain-containing protein"
                     /protein_id="HPX33725.1"
                     /translation="MRKISLLILLFIGLTVYSQDDASQQEAPSRWKRNGIASLQLNQA
                     SFNNWSAGGENSYAFLALMKYYADYAHENFSVDNNLNLKYGLQSVGKNALRKNEDLIE
                     WNTKINQKFSKKWSASGLINFTSQFANGYNYPDDSTVISKFLAPAYLTIAPGILYKPV
                     DYFSILFTPASLRAIFVTDQELADLGAFGVDPAEFNEQGAKVTDGKNSKIKLGAFAEF
                     YLKKNVKTDLDLESKLNFFYNYLPDNALPSGKIPLDINWQNFVNYRISKWFSTTFFIH
                     LAYMPTDIRIESIPPPSGIGGNELKVTPNNKVQLKQTFGIGIAYNF"
     gene            19238..19636
                     /gene="mscL"
                     /locus_tag="PK345_01485"
     CDS             19238..19636
                     /gene="mscL"
                     /locus_tag="PK345_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HPX33726.1"
                     /translation="MSIIKEFKEFALRGNVIDLAVAVVIGGAFGKIVSSFVADVLMPP
                     IGLLLGGVNFTDLVITLKKAQGDVAAVTLQYGNFIQAVVDFLIIALAIFMMIKAINLA
                     KKKEEEAPAPPPEPTKEETLLTEIRDLLKK"
     gene            complement(19769..22873)
                     /locus_tag="PK345_01490"
     CDS             complement(19769..22873)
                     /locus_tag="PK345_01490"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPX33727.1"
                     /translation="MKKFNFSNQVKVSKNTMLTLNNSRDQIVTSNNKQASLFTKLIDL
                     KNWHVFSPRFLLIIVIALFAYSCSENHLSITSYVDPFIGTDAHGHVFPGATLPFGMVQ
                     LSPDTRKDDWDGCSGYHYSDSTIMGFSHTHLSGTGVGDYGDIRFMPTVGKVILNPTKD
                     ENNESGYQSKFSHKTETASPGYYQVLLNDYNINVELTTTQRAGFHKYTFPKTENANII
                     IDLFEGVTSDQILDLWIRFVSDTEIEGLRRTKGWADNQWVFFNAEFSKPFAGYGISVD
                     NKIDEGLQYAQGKNIKAWVNFQTKDEEIVLIKVGISAVNTEGAKLNRMQEIPSWDFEQ
                     TKTMASQKWEMELSKIKAESNDKNLKKIFYTSLYHSMIAPNIFTDTDGAYRGHDMKIH
                     ISQDREIYTVFSLWDTFRALHPLLTITHQQLTSDLINTMLLHHKQGGLLPVWELAANE
                     TNCMIGYHAVPVIVDAYMKNIRNFDVNLALQACVKSATQSHYGLDSYKTKGYIPSDAE
                     PESVSKTLEYAYDDWCIAVMAKEMGDTETYDDFIMRAQYYKNIYDPETGFMRAKNNET
                     RFTPFDPAEVNFNYTEANSWQYSFFVPQDISGLMELMGGKENFVQRLDQMFTESSVTT
                     GRHQADITGLIGQYAHGNEPSHHMAYLYSYAGKAWKTQEILRKIMDNLYTHHPDGLCG
                     NEDCGQMSAWYVFSAMGFYPVTPGSPFYTIGTPMMDKITISLENGNTFVILAKGLSKE
                     NKYIQKATLNGKIWPNSYISHQQIMNGGLLIFEMGNKPNKSWGTSEEQIPITHITDKL
                     ITPVPFIKSGNSVFFDTQTIELACADPLAEIKYKIMNDENNTFIQYTNPITIESTTII
                     KAKAKRNDQIDSFIIEAKFVKIEPGRTITIKYPFAPQYSAGGNIALIDHQHGGNDFRT
                     GRWQGYEGVNLEAVVDLGKTMRISTLSTGFFQDVNAWIFMPEFVEYSISDDGKKFRFI
                     GNVKNPISEDDWNIQSHDFTLKFAPISTRYIKIIGKNRGYCPDWHKGAGNNAWIFADE
                     IVIK"
     gene            complement(23091..23387)
                     /locus_tag="PK345_01495"
     CDS             complement(23091..23387)
                     /locus_tag="PK345_01495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33728.1"
                     /translation="MKKQAHPFLIKCPKQIEYRGYDSAGLTILEKKIIVYKTKGKAND
                     LENYIANKNTEVFISIIIHTRWTTHGEPNEIYTHPHLSRANKLTLIHNGILENF"
     gene            complement(23422..24807)
                     /locus_tag="PK345_01500"
     CDS             complement(23422..24807)
                     /locus_tag="PK345_01500"
                     /inference="COORDINATES: protein motif:HMM:NF025458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4270 domain-containing protein"
                     /protein_id="HPX33729.1"
                     /translation="MNFKFNSGSTSSNRNNHFFLLVSIFSIIVIVFTNCKNDFDKVGI
                     GLIPGSEILELFSSDTTTVLVHSVFADSVKTDNTATSMLGSYLDPIFGLNTISIATQV
                     RLSTVNMNFGENPELDSIVLVMQYSFYSLPNFQPVYYYGDTATTQTIKVFELDESLYY
                     DSTYYSNSLVATKSTELASMDINFSPRDSIMIDSVKVKPQLRIRLNDEFGNNILNAPA
                     SVKESNENFQDFMKGLYIKPEPVANNGVIAFFDLLDINSCLNLYYKNDEGDAKMYSFL
                     INAACARYMNFTHDYSMGDPDFVAQLNGNHDLGKEKVYLQSLGGVETRISFPYIKDWF
                     KDQKIVINEAKLVFHNVDTNSIYKPPHELVYYFVKDDGTLGFIEDQFEGVSYFGGQYD
                     KKKGEYFFRLSQSLQKIMLGEKENTVFTMGISGASLSPTRVAFHGTWPNDENLMNKRI
                     KLQIKYTKLTN"
     gene            complement(24807..25637)
                     /locus_tag="PK345_01505"
     CDS             complement(24807..25637)
                     /locus_tag="PK345_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008148795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HPX33730.1"
                     /translation="MIPKAEKIKVLYVMQEITPYLDESPLSNIGRYLPQGTQESGKEI
                     RTFMPKYGQVNERRNQLHEVIRLSGMNLIIDDADHPLIIKVASIQQARMQVYFIDNED
                     YFQRKGIFHDKNGQFFEDNDERLIFYARGVIETVRKLGWPPDIIHCHGWFSSLVPLYI
                     KRVFKDNPLFSSSTVIYSLYDDFFEDMLNHTLVKKLKFDKIKEVDLKNYRNPDYIKIS
                     KAAIKYSDALIFGSEKINPELEELVRSTEKPLLEFLGDDNYITVYNDFYEKLLKHKRN
                     "
     gene            25872..27677
                     /locus_tag="PK345_01510"
     CDS             25872..27677
                     /locus_tag="PK345_01510"
                     /inference="COORDINATES: protein motif:HMM:NF025064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HPX33731.1"
                     /translation="MIKKDIDYSILDGKRFYYSFLAGSQRLFDNQVLLNKINVFPVKD
                     ADTGTNLVSTVRSIIDSIIPSENFAVTVNAVADAALVGARGNSGIIFAQFLYGFSSEF
                     KDEQTIDITAFSTAINRGVRHSYEAISNPVEGTMITVIREWAEYIQKIKDKFNNFNQL
                     ITESYNQAKQSLIETQEKLEVLAKAKVVDAGAKGFVVFLEGMIDFFRHGELKKIIGAR
                     NLVKTTALEGAHDHENITYRYCTEAMLVGDDIDREILRSKIDNLGDSLVIAGSPKKMR
                     IHIHTDTPDVLFSIINNFGNITFQKVDDMIMQMDIAENRKYPIALVTDSTCDLPQEFV
                     EKHQIIIVPLAIHVGDTYYLDRLTMKSKKFFELLNTFPVYPSTAQPSFKDFSNTYNYL
                     STHFNSIIGIHLSETMSGTYSNSRKAAKTISNFTKKPISVINSKKLSGALGLILMRAV
                     DALQDKSITHDELVAQIEQWCDKTDIFVTTKTIKYMVKSGRLNAMKGFIGKLLRVKPI
                     VTVNNKGIPELFGKPLTEKSSRKMAVRTIEKKIKSRKLWGYSITHANNPSTADWYARE
                     IEKIIGLKPCFIQDGSPVLGVHTGPDVVAVSLMYE"
     gene            complement(27685..28929)
                     /locus_tag="PK345_01515"
     CDS             complement(27685..28929)
                     /locus_tag="PK345_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX33732.1"
                     /translation="MQFQIFIENIKISLEAIRSHILRTILTVLIIAFGIMALVGISTA
                     INALESSLAENLQMMGSNTFTIRNRSMQIQVGANIHKAKYYEPIRYRQAMDFSKSYEY
                     PATVSVFTYATWGATIRNRSLATNPNIAVIGTDENYFATSGLELQKGRAFSNHEMAMG
                     TNVVVIGFEVAKNLFVNKENPLDKFITVGPGRYRVIGVIKEKGTGMGFTPDRQAFIPI
                     TNARHFFSRPNMTYNINVMPAEITNIDDAIGEATGLFRIIRQNKLYEENDFDISKSDQ
                     LLQMLNENTKNIKVAALIIGLITLLGAAIGLMNIMLVSVTERTREIGIRKSLGATRLN
                     IRNQFLVESILIGQMGGILGIIFGILAGNLVTIFTESSFIIPWAWILIGISLCFGVAL
                     LSGIIPAIKAANLDPIESLRYE"
     gene            complement(29001..29882)
                     /locus_tag="PK345_01520"
     CDS             complement(29001..29882)
                     /locus_tag="PK345_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HPX33733.1"
                     /translation="MKIVGFYIAFVLIWLLTLLPLRILYLFSNLFYFIIYYIIGYRKK
                     VVIDNLQHAFPEKSKSEVITLSKLFFRHFCDILVESAKTIHMSPQEIERRVTYPNPEI
                     LHKYFDRGQSVVLVSGHYGNWEWMSNTPRILKHKALAIYKPLQNEQFNKLILKIRKRY
                     APQIELIVMNEVVRVIIQQLKNKQVILTWFLTDQTPPRNYPFRTEFLHREVPFYTGFE
                     KIAKKFRHAVVYSQMTKIKRGHYKVEFIPLFDDPAATQEYEITKKVVSTLENTIKQQP
                     EYWLWSHRRWKHSKEKP"
     gene            complement(29895..30545)
                     /gene="upp"
                     /locus_tag="PK345_01525"
     CDS             complement(29895..30545)
                     /gene="upp"
                     /locus_tag="PK345_01525"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HPX33734.1"
                     /translation="MVNNLGKGNSLLNQYISELRDVEIQKDTMRFRKNLSRIGSIFAY
                     EISKKLEYKSREIITPLGTSEMKVLKNYPVVASIIRAGLPVHQGFLDFFDRSENAFIS
                     SYRKYYKDGNFDVHVQYVSKPSVQDKDLILVDSMLATGVSMALTYRELISDEKPRHSH
                     LIAVVASVEGVNYIKRNLSSKNVTIWVGAIDDELTAEAYIVPGLGDAGDLAFGVKP"
     gene            complement(30816..31115)
                     /locus_tag="PK345_01530"
     CDS             complement(30816..31115)
                     /locus_tag="PK345_01530"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 family protein"
                     /protein_id="HPX33735.1"
                     /translation="MKQFSSFKTIGGNGHNKLVFPVTFDIKIIIESIHPIEQSNAYLR
                     AIFSQLRIPNSNWRTMHSAEGKYVSFTIQVDLKDNDLMQKLYSELKTIPGIKLAL"
     gene            complement(31161..32423)
                     /gene="aroA"
                     /locus_tag="PK345_01535"
     CDS             complement(31161..32423)
                     /gene="aroA"
                     /locus_tag="PK345_01535"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HPX33736.1"
                     /translation="MDALVYPSFVKGEVNAPPSKSYTIRAIAAAMLAAGKSTIINPSS
                     CDDALAMLNIAHSFRAEIKFNNQNLEIQGGLKSNPEIIYCNESALTARLMIAIAALSE
                     NKITITGSGTLLKRHLGNVNDPLEANGVKFTSNNGFLPVSVQGRLKPGYYLVDGSESS
                     QFISGLLMALPLLNNDSQLIVNNLKSAPYLKMTLEVLRAFNVNIETKGTDFYLISGNQ
                     AYCPQNFIIEGDWSGAAFPIIAALLNGEIDITGLLPHSLQADKKLNTILEDIGIKTVC
                     ANNKLYLKKSEIPAFVADCTHTPDLVPALVILASQSAGKCRITGASRLFYKESNRALA
                     LKLEMSKLGIEIIINGNEIIIYPGKIQPGEVSSHNDHRIAMAMMIAGLIAEGPLLVKD
                     VGCISKSYPQFIDSMQKCGARIETILSD"
     gene            complement(32433..33479)
                     /gene="aroB"
                     /locus_tag="PK345_01540"
     CDS             complement(32433..33479)
                     /gene="aroB"
                     /locus_tag="PK345_01540"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HPX33737.1"
                     /translation="MNSIKSTEIFSNGKISQIITGDKISNLSRYLPPETVVITDVNVN
                     HLYGGYWSNWPNIAIGVGEKIKTLDTVNEIYRRLLEYEVDRSGFIVGVGGGIVCDIAG
                     FVASTYLRGIRFGFVPTTLLAQVDAAIGGKNGINYNGFKNQIGTINQPDFVLCDPVVL
                     QTLPKELLADGISEVIKHALIAEEKLLDFIENNYNAILSLDLQTIGELIHHSVKIKTE
                     IVNQDEKENGERRKLNFGHTLGHAIEKVTGINHGKAVSIGMLTAAEISTKKGLICSDD
                     LARIQSLLKLMNLPINCPSNRLEIIDAVAKDKKRQAGQIYFILLAKLGKAIIQEIGFA
                     DLAEYIKESKFLGS"
     gene            complement(33674..34117)
                     /locus_tag="PK345_01545"
     CDS             complement(33674..34117)
                     /locus_tag="PK345_01545"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPX33738.1"
                     /translation="MKKSIFLMMLTIMSVTIIFAQSPVKTTKSEESKTVSTDTEVEIG
                     PKAEWAATTIDLGEVQFMVKKDASFTLTNTGNQPLLITSARASCGCTNLKYSQEPILP
                     GKSTELSVTFNGSGNGAFRKSITVQTNASENPTILQITGKVVKNN"
BASE COUNT        10490 a         6332 c         6445 g        11017 t
ORIGIN      
        1 catataaaac cgtttgtaag tgtaacaata aaattgtttg gctgaggcag tacctctctt
       61 atacctgaca tcagtttttc acatcccaat tggtttcttg ccttcacttt gtatttgcca
      121 ggattattaa cttctttaaa atatattgca cctcctgaac cttggattgt atccactagt
      181 ccttctgttt cactccaaag ttcataagtt acactatcct gtgaattagt caagtaaaat
      241 gaaactggcg gacaggaagc ggttgcaggc atgagtgtat aagacactgg tggagcgcca
      301 attataacag cgccaaccat atctgtccag cagtcggtat tttgatggga ccacttagct
      361 tttacagaat aagtaccggc ccctgtttgt gttttaaaat gaacagctcc tcccgtacct
      421 tttattgaat ccatcaggtg catattattc tggtcatctt ttcgccatag ataataagta
      481 acttgtgctt cagaatgttc gagatagata acaacaggtt cacagtctgc aaatccctga
      541 ggctgaacta taaaagcagt tgggttaggt aaaatagttg tagaattctc catccaactg
      601 gtgcaattgg tgattggatt aacacctaaa atatgataaa ctcccgcatc agcccaaact
      661 ccaaaattaa tagcgctatc ttcgttacaa actaccgggt gttcagttag ctttatccaa
      721 tcgggtgttt gtttatccgg gaatgattga aaatagagat aatattcaat acctgtctgg
      781 caattattaa gggatatctc agctcctgaa caggcaaaag caggggacat gcaatatttc
      841 accggatttg ggtgtacgat tatctactgc tcaaaaacaa tctcgcagag tgagttaatg
      901 ttttttgcat aaaccctata tactcctggt tctgtctgct cctcgaaaca aattgcatca
      961 ccagtcccgg tgatgattgc tccaaaagga ttgttgtttt tccaaagttg atagtttacg
     1021 ccaatctccg aaccttcaat gcaaatcgtt gaaccttcgc atagtattcc actggagtaa
     1081 attggaaatt ctaacggaag tggtttaatt atcagctcac catccatcat tctctcacat
     1141 agagagctga ggtttacagc gttaattgta tagataccaa cctcggtttg ttccttaaac
     1201 tgcagaaaac cgatctcacc ggtaccatcc acctcatcta caatagactg cccatctttt
     1261 atcagttgat acctgacacc agcctgtgat ccatttagca gtggcgtaac cggagcgcat
     1321 tggttacccg aaggaacaat cacaaatata cttggtaggg gattgacaat aacagctacc
     1381 tggccgatca tctggttctg taccatattt gactgattga ttcctacgac tgtataaata
     1441 ccttgcttgg ttatattgct ccattcaata ggattgccat taccagcctt aggaggttga
     1501 accggtattg tgccatttcg gtataactga taattgaccc ctgtttgaga accatttaaa
     1561 taaacattca aggaacctcc ctggcatatt actccaccac cggtaacagt aaattggata
     1621 ggttgctttt gcacatagat attatactga taggttattc cctgacatcc atcgaaatag
     1681 ggcgtgatgt aaaatgtgat aatacaggtc tctggcccaa tcgaatcaat ttcaattatt
     1741 tgagaaggca atacatctcc attgagaggt gttgagtagc tgagcatctc cgggcagtta
     1801 tcttcggaac tccactgaaa agtagctcct gaaacatttg aggtaaatat cacttcagcg
     1861 cttgactgac ctgacacaac cgtttgcgat gtaggcgagt tgacaatctg agcagtggga
     1921 tttaccaaaa cagtcaactc aaaatcagta cctgaacaaa atgttggttc agggccggta
     1981 ggttggataa aataggtaat gctttgaact tcatttgagg agttaaataa agcatcgttg
     2041 atatctccaa cacccgctga atttccgtaa atgtaaggat tggggactga ataccagtta
     2101 aacgttgcct ctggaatgtt ggcttcaggc gaatagttaa gctgttctcc tgagcaaatt
     2161 ttaataacag acgaattatt aataacaaat ggtaaatcgt caacctgaac aataaactca
     2221 cactgtgtgg tggttgctcc atcatctacc tccaataaat aggtatttga acctgaattt
     2281 gttggtgtat gcattatttg ctggccgctc gaactacctc cttcaggaag actccataaa
     2341 taggtataac tacctgatcc cccagaggct gaaccgttaa gtgtaaccga ctcgccgata
     2401 caaatatgat ttttatctgc cgtaactgag cagatgagcg atcctccaga tatatacaca
     2461 gtcatattat ctgtgctgga acaacctgtt atagaatcag taacagtgag tgtaaataca
     2521 ataaaatttg ttggtggagg atataaattc actgttgtag gatttagtgt tccctgaccg
     2581 cttgaaatta ggtctttggg ctcccaccaa taggtgtaat tccctgaacc tccagaagca
     2641 gttccgttta aagtcacctg tttgccattg aaagcgttta tatcatcacc ggcatttact
     2701 attggttttg ggttaactgt aaattcaatc acattgctct cattgttatt acagtttacc
     2761 ggaacaggag ccggagcttt agcaattctt ctgaatctgg tggtatggtt aagctgaggc
     2821 aggagataat cctttgtgtt tacatcaggg acatctatcc aattccaatt aggagccact
     2881 gcctcttgcc aggtgtactg ccatgacgaa ttatgtcctg attgggaggg gtctgaaccg
     2941 tatatggttc ccgcaggctc gccttcacaa ataaccggat cgggatgata gatcgagtcg
     3001 ttttctatat caccgtaaac gttaaccgta acaatttcgc ttatatcctc acataaaccc
     3061 tcaaccgaat aaacgatcct tctgtaacat gttgtaacaa aaagctctgg tggggaaaaa
     3121 gttttattat taggttctcc gttagcaggt ccccaggatt ccggatcgct acaatctgtg
     3181 ctctgttccc acaaatattc aaaagttcca cccccgcctg caggatctga tcccataaga
     3241 gtaccaggcg tttctccaga acaaatgtgt tgtaccgttg gttgtattga attgttagta
     3301 attgcagaac gaacagtaaa acttaattca ttggaaacag attcacaccc ggccgatttt
     3361 actattcggc ggaattttgt ggggttgacc gtaagcgttg aatcgtagtc taaatcaggt
     3421 gaattttcat cattaatttc tgtccagcct gacccttcgt caatttgcca ggtataggta
     3481 tacgtaccgt caccccctgt taatggagga gttgaatttt cgttggttcc agttaaaact
     3541 tcactcaatt tcatatttgc gcacaattgc atatttccgt aatatcttat agagtcattc
     3601 tcgatgccag gacttacatc aaattgcaaa gttgcaggtt ctgattgacc acattcattg
     3661 gttgccacaa ctgttatgtt gtatatacca acatcattga atgtgatggt tggataggct
     3721 ggattgttag tattaaaaat cggacctaag ggatccgaag tccactgata ttgggttatt
     3781 gggctaaaat tatcgttata actgacactg tctggttgaa tggtaaaatt tttcccaaca
     3841 caaagaaaaa tggagtctcc cttaatttca acttctggcc gttggcaaac taaaatagat
     3901 tttgtgattg tatctgaacc gcaactattg gtaatggtta gcgtaacgct gtacgtgccg
     3961 gatacattaa actgaaatac cggattgata tatgtattat ctccttcatg ttcgggggta
     4021 ataatttgcc agtcattttc aggctgcact gtccaaaggt attccggcac gccaaagcat
     4081 cctatatcgc ttgtgttatt catctcgact tcctttggga cacaattact ggtgaaatcc
     4141 atatcgaaat tggcagtagg catctcctgt atgtaaacca gcttttgaat agtatcaata
     4201 ccagagttgt tgatcgaatt ttcacccgtc caggctatta acgtaaccgt gtatgactcg
     4261 catgagttgg taaatgtatg tgtaggattg gggtcactgg aaaagttgtt ttcacctgat
     4321 tcaggatctc caaaatccca tgaatagaca gtattttgtg taaatgttgt tccatttccg
     4381 gtaatataaa accccggaat agtctcgttt gtaaaggtta cgggattaca ggccataatt
     4441 tctccttgcc cggcgcccca gcccaatcca acgccaaatc caggtatcac ggggcatgaa
     4501 acataaattg ggcaagctat tgaatatgca ttatcacagg gatttgaagc cacgcatgat
     4561 gctttgaaag tatatctacc ctcccatgga aaaagacaat agttccactc atcagaacac
     4621 ttagtatagt agtgagcaat tttcttatcg gccggtagag tactttgagt aaaataaaca
     4681 ggatcagtac catcaccaaa atcaaataca taggttgttc ctagtgtgtt atcataagtt
     4741 tcttcagcta tcgggaactc cagataaaaa ggcacattac ctgatgcatt ccctgggtta
     4801 cctatacctc cggagggtgt gcttccaata aaaatataat acttcttaat gtgattgcat
     4861 ccgttttcct ggctaacagt atatgtcaat gtcttgaagc ctaatccgga atattcgtga
     4921 taaattggct cattgaattc tggtgagtta aaagccggag aatcatctcc ccaatcaata
     4981 tgatacaagg tattaatgga tggcgttgta gattgattgg taaaagtaaa agttgcaaat
     5041 gttacagatc cacaaaatgc gaacgttgta gtgtcattaa ccatagtagt tttgattcca
     5101 tccccactgg cgctcagagt agcatcaggt ttttgctgca cagtaacctc tttaattatt
     5161 tcagcttcac atccattact atctgtaacc aggagtttaa catcgaatgt ctgagaaccg
     5221 tttccccatg cttcaaaaac gtgaataggg ttttgcagcg ttgagctatt cctactacct
     5281 gaattagggt cgccaaaatc ccacaaccag gaaacaatag ccgcttcggc ttggttagcc
     5341 ttagattgat tagtaaaaac caccggatca tttgcacact gattttgggg agatgcagaa
     5401 aaattggcag tggggttgga gaacacagtg atagaaacac tatctttcat ataacttgaa
     5461 caattgaagc gtttgcctct aacagtatac gttcctggta tactaaaata cccaaatgaa
     5521 ataggttgac cagtaccagg ctcattagta ttattaagaa gactaccatt tctgtacaag
     5581 aaatagttaa cacttttctc acttccattc aggccaattt caattgagtc agtaattcct
     5641 gcgcaataac cacccccacc agtgatttta aaagtatcca gaggataatt atcttttcta
     5701 acgtagcgtt cattagaagt gtaaaagggg tcttccacca tgtaacgata ggttccgatt
     5761 ttccaaacac catcaaacca gacagttttt ccgtcccctt tctttgaatc tttagttacc
     5821 caactcccat tcttttgctg aaggtgatag gttgttcccg ccacggtatt tgtaacacca
     5881 agttgtaaac cttgttcacc atcgcaatac ctgaagttgg gtgggttact tgtaaatacg
     5941 ggttgattct gcgcaaaaat atctaaagtc aggataataa gcgtgcaaaa tgtaataata
     6001 agttgtaaaa aatttttcat ataaaatcag cgttaaagtt ttcaattcaa tcaaaattca
     6061 aaacatagta aaattgggta aagttgcgaa acataacatg agcgtatcat ttcagacatt
     6121 gaataaaatt tatgacaaca gtcgttaatg tttaatttta agaaagtcgg taaatctata
     6181 gtaaaaaaac tttactggct aaattatggt gaaatttaga attagattaa tttacttaat
     6241 cacagcagga aattatttca aaatcagatt aacctactct aaataccttg tcacaattgg
     6301 catttttctg cccataccaa aagcttttgg cgagacgcga agtattggcg gggtttggtg
     6361 gcgtttgtat tcgctaatgc ttacgagcct caacgttttt gtgatcagct ctttgtcaaa
     6421 ccccatttcg atcaattcct gataactttt atgtttttca atatggttat aaagaatttt
     6481 gtccaaaaca tcataatccg ggagtgaatc ggagtctttt tgattggggc gcagttcggc
     6541 agatggaggc tttttgataa tattttcagg aataacctct ttgtagtaat ttatatatcg
     6601 ggcaagttca aaaacttcgg ttttatatat atcgccgatt actgataatc ctccattcat
     6661 atcgccgtaa agtgttccat acccgactgc tgcctcactt ttattagaat tgttgaggag
     6721 aagataacca ctgttatttg ccattgacat caaatagaca cctctgattc gtgcttgaag
     6781 attttcgtcg gtagcgcctg taggtaggta accaaatact ggctcaagcg tttgtctgaa
     6841 taagttataa gcttcgctta tattaatgat tttgtacggc gatttggtgt ttttaagcag
     6901 gtggattgca tctgttaagg agtgatcgga tgagtaaggc gaaggcatta aaaccgacat
     6961 tacattttct ctacctaaag ccttgatagc taataaaaga acaaccgcag aatcaagccc
     7021 gccagagaga ccaaggatgg cttttgtaaa tccttgttta ccataaaaat ccttgattcc
     7081 cattatgagc gaatcgtgga ttaaacctat tttcagatat tcgggaggat aattgtcaat
     7141 ttgcttaggg gaggtggtag tagtggtaac tttagtatca ataatagtaa aatcctcttc
     7201 aaaaaatttt aattcatata accttttacc tgttttgtct aaagccaatg agccgccatc
     7261 gaacaataat ccaccttgtc ctcctacatg attcacataa ataagcggga gtttatattt
     7321 taaaacatag tttgttaaaa tttccgttcg ttccttccaa aggtcataat gaaacggggt
     7381 agctgcaata ttaataatta tggtggggct ttcttcggca agtatatcta acgggcctga
     7441 ggcgtaattg tcaatgttcc acaaatcttc gccaatggta agtgcaatct tttctccttt
     7501 aaatgatatt gttccccatt ttttattcgg ctcaaaatag cgatgttcgt ccttgctatt
     7561 attggaggct aaaagagttt tgtgtcgaac ttcttttatt gagccatttt ggagaagaag
     7621 ggcagagtta tagagccttt ttccaaattc atcattattt acataaggcg cgcctattat
     7681 tgccgctatc tctgtgcact caacagcaat atcggctatc gcttgttgac atttaccaac
     7741 aaaagaatcg taatgaagta aatcataagg tggaaaacca caaacagcca tctcggcaaa
     7801 aactattaaa tcaacctttt gcttcttggc agattgaatt gaatgtttta ttttagagat
     7861 atttttttca aaatcaccta catagtaatt tagctgtgtt agtgcgattt tcataaataa
     7921 cttaaaatat catcccgaaa gtaatttcaa aataatttaa ggtggcttta ggttttatat
     7981 cggtaacagg atttctgtct tttaaaacat tggtaaatcc gtttgagtaa tttaaaccaa
     8041 caaagaagca ggtggattga tcaacataat attcggctcc ggcgcccaat atcaggcctc
     8101 ctcttattga gttgatctcc ttttttatgt tctcttccgt gaaatatttt tttgctgttg
     8161 tagtaaattc atctttagca tcagccttta ttttgatacc taaggtaggc ccaaactgcc
     8221 cgaaatatct aaaattgtca aacctatttg tacacatttt tagcattatc gggatatcca
     8281 catagctgat actatacttt ctatgcaggc tgccagtaac tttttctgaa gctccttcca
     8341 ccatgcggct atcttgatat tttaatttcc catgtaaatt gtttacggca aatcctgtgg
     8401 acaggaaata gttgtcggca agggtaaagt cgccaatcaa tccccagttg tagcctatac
     8461 cccccccgtc tttttcataa ccattcgaac tggttgagag ccaaccaaca tttggagaaa
     8521 tcttaaggcc aaatttaaaa ggcttcatct gcgcaaaaac agcgcttgat gagattagta
     8581 aaactaataa cgttgttaag taaattcttt tcatgattaa atattttttc agcaaaaata
     8641 gataactttc ttataaatgc ttttggtgtt atattgaaaa cgacaaattt gtcgtttata
     8701 gtgaattaaa ctggtcaaaa tctaagccat gaaaaaaata ctttttgttt tattgttgac
     8761 attgtcggca agtttggctt ttggtcaatt tactcttggc ccaaaaatcg gttatacaac
     8821 ctctaaactt tcaaccgaat tggaagatat tcattccagt attaaagaag attttgattt
     8881 tggtcttttc atgcgtatag gtagaaaggt tcatctgcaa cctgagatta tctggacaag
     8941 taaaggcgcc cacctcgaac gtactgagaa ttccaattta aaagccatca atcaggaaat
     9001 taaattgaaa actattgaca taccattgca tcttggtttt cgccttgtaa actttggtgt
     9061 tggtaatatt agatttttgt ttggcccgaa tgcctcgatt gtagttgata aaacggtttc
     9121 gatacaagaa aaagaagagt atatcaatcc cataaaaacc gctgatattg aggatctgat
     9181 ttgggcagtt aacgttggcg gcgggataga tttatttatg tttacaatag atgtaaaagc
     9241 tcagttcggg atcaatgata ttgtagaaca ggcagataat attgactttc atgcaaaaac
     9301 caatcttttt attgtctcct tgggctggaa gattttttaa caacaattag caatgtgcaa
     9361 ttagcaatta gcaattaaca attagcaatt ggcaattagt aaatcgcttt tgttttagat
     9421 gcttgtaatt ttactttttt gataatgcga aatcctaata aactattatt gttttctgtt
     9481 tttatacttg tagtaataat catatcggct tgtaccggca gaaaaacacg tgatttgcat
     9541 gtcaacttga aaggtgttga aatagagccg ataaaaatta aaaggtacga aaaagcgtta
     9601 tttaacattc ccccagaagt tctcaaaccg gaattaaaaa agatttcaac cgtttatccc
     9661 gtatttcttg attatgacct tgatgatacg cttattataa tacagttaca taggtttatt
     9721 accgaaccag tgaataggga gctttatcag tttactacgg atagataccc cgagcttaat
     9781 tttttagaaa acgattttca attggcgttc agatatttta aatattatta ccctgataaa
     9841 ccacttccca atattacaac ctatgtttcc ggattattat atgagcttcc tattcaattc
     9901 tttggtaatg agatggtcat tgcacttgat atgtaccttg gagatgatgt agagcattac
     9961 cggcgttttc gattcccact ctataaaatt gaacgtatga attcagatta tatcgtaaga
    10021 gacgggattt atgattttta ttatcttcat tttcttaaaa agcccggaag taatatgctt
    10081 gaaaagatga taagtaatgg taaacatctc tacttcgttg atgctatgct tcctgatgtg
    10141 cctgattaca ttaagattgg ttatccgccc gaaaaacttg aatggtgtat agctaacgaa
    10201 agtaacattt gggcatttat aattcaaaat gaattgcttt atagtggtga agccggtaca
    10261 ttacgtaagt tttttgctga cgggccattt actaatcaat tcggacaaca atctcctgca
    10321 agggtaggcg aatggatagg ctggctaatt gtaagaagtt atatgaataa aaacagggag
    10381 atatcgctca atgaattgtt ggataatgat gatttccaga tgatttttaa taattccgga
    10441 taccggccgg cgaagtagaa tttcaaaacc aaattaatca acagaaagtt cttttgcgtt
    10501 aagcaaatgg ctttattggg ctaatcccga tttagtgttt gtttagcaca attcttatca
    10561 tgttgacttt aaaaacttaa tctggtttat tgatgaattg atttattttt ggcaaaatat
    10621 tcagaaatac ttttgcaaat atccatccga gaaaagcgcc taataaacta ccagctaaaa
    10681 cgtcaccagg ataatgcact ccgagataga tacgagaata acaaatcgtt gaaacccata
    10741 gtatcaatgc ggcaggaaaa aatttccaat aactttttaa aaatggtatg ataaaaaata
    10801 caagagcaaa aaaatttgta gcatgcgaag aggggaaact atattttcca ccacattttc
    10861 cgtaaacggt atgcacctgc atttgaagta taggatcatg acagggacga aatcgttgca
    10921 caagattttt tattagaaca cttaactgat cgctgcaagt aataaggata gctactgcca
    10981 acaaaatcca taaggtattg attttgtatt ttttaataaa taaaaaaaga aaaaaaagat
    11041 aaagcggaat ccagattaat ttatcgctaa cccaaaacat gatcagatca aaaaaaccat
    11101 tattcagtcc gttcaggata agaaataagt tgcggtcaac attaatcagc caatcaacca
    11161 taagtagtta tgatttatgt ttattaagaa tttaactcct tccaaatcat gtcttttagt
    11221 ttcattaatc ctttttgtgt gattgaggat atgaagacgt aaggcattgg aggtaggttt
    11281 tttttaattt catctatcaa ttcctcatct aacaggtcag attttgagat ggcgaggata
    11341 tgcgacttat ctaacagctc agggttgtat tgctttagtt cgttcagcaa tattttatac
    11401 tcctttttta tgttttttga gtcggctggt atgacaaaaa gcaggattga gtttcgttca
    11461 atatgtcgta aaaaccttaa accaagccct tttccttcgt gagcgccttg tattatccca
    11521 ggaatgtcgg ccattataaa cgactggtga tcgcggtaac caaccattcc aagatttggc
    11581 ttaagggttg tgaacggata atccgcaatt tctggtttgg ctgctgaaat agcggcaagc
    11641 aatgtagatt ttcctgcatt gggaaatcct actaagccaa catcagctaa aagttttaat
    11701 tcgagaattt tccattcttc ttttccagcc tcgcctggtt gggcatatcg cggagcctgg
    11761 ttggttgccg atttaaaatg atcgtttcct aatccgcctc tacccccgta aagtagcact
    11821 ttctcttcat cctgctgagt gatttcgaat tcaatctcac cggtatcggc attgcgggcc
    11881 acggtgccca atggcacttc aaggattacg tcttttcctg atgcgccgct ctttagttgg
    11941 gagccgccat ctccaccacg tccggctctt acatgtttag tgtatttaag gtgtagtaat
    12001 gtccataatt gttgattacc cctcaggatg atgtgcccgc cacgtcctcc atccccaccg
    12061 tcagggccgc cacgcggatt cagacgcgat cggtgaaaat gagctgagcc cttgccacca
    12121 tgaccggaac gacaaaaaat ctttacataa tcaacaaagt tggatgaact cattatttat
    12181 caataatttt gtaaatatcc ggatagttac gaccgaaacc atcataatct aagccgtatc
    12241 ccacaataaa atcatttgga atctctatgc ctatatagtc aatcttgaaa tccttttgaa
    12301 aagctttggg cttgaagagg agcgttgcaa gatacacctc tgcagcctca agtttatata
    12361 gcttattaat ggcttcggcc atagttaatc cagtatcaat gatatcttct acaataacga
    12421 tcgtccggcc atgtagtatt tcatcaagcc cgataagttc acggacgata ttggttgttt
    12481 tagttccgga ataactcgaa agcttaacaa acgatacctg acaatctatt ttaattcgtt
    12541 ttaaaagatc ggcagcaaaa atgaaagcgc catttaacac tacaagaaaa agtggatttt
    12601 taccatcaag gtcaacggaa attctatcgg caagtctttc aacagctttt tgaatagttt
    12661 cggatgagat aaaaagttca aaacttttat cattaacgtt tattgttggt aaattctggc
    12721 tcatgtttac tatttttagg caaagatact ctgatatatg tatgccggtt ttaaaaaaat
    12781 gtaaagatta tctttaattt tattatgatc aagatcaaaa aatgaaaata ctactgtttt
    12841 acgaaaacct tttcttgaca atggagcgat aataaacatt gcagattccg gaatcccatt
    12901 tgatgatgta gatgctgaag cggttgtaac tccttctttt tttagtagag caatggtaat
    12961 gcggacaaac acgaattaaa tcattagaaa gcctattagg ttaaaccaat gtttttatta
    13021 agtcaatttg ggttttgcat ctgtttgctg caactcttat tgtgttgatt ataaatgttt
    13081 gatcattgaa ggattttcaa ctatttgatc cgtgttttat aaagttgttt atctctttaa
    13141 acctccttga ttattaattt acattaaatt tgtccactga cttaatattt aaattatcat
    13201 tttaaatgag gccaaaagta agtatcatta tgggcagctc ttccgacctt cctgttatgg
    13261 aagctgcggc caaactcctt aacgagttta gaattccttt tgaaatcaat gctttatcag
    13321 cacatcgcac tcccgaagaa gttgaatcat ttgctcgttc ggcatacgat cggggggtta
    13381 gagtaataat tgccggtgcg gggatggcag cacaccttcc gggtgtaata gctgcaatga
    13441 cggccattcc agttattggc gtgccggtga aatcatcact tgaagggctt gatgctcttt
    13501 tgtctattgt tcagatgcca acggggattc ctgtagcaac agttgcaatt aacggcgcag
    13561 gaaacgcagc aattcttgca gcacaaattc tatcaatatc cgataaagag ttatataata
    13621 gattggttga atacaaagaa aagctgaaag ataaagtgat caaagccaat gacgatctat
    13681 ctaatattaa atacgagttt aaagtataaa cctgttttta atcagattat caatgattta
    13741 taaggaagaa atcgcattta aaatggccga actgctttta aaaattaagg cagtaaagat
    13801 taatgtagat aatccgttta cctgggcttc cggactcaga tcgccaatat attgcgataa
    13861 taggattacc ctttcttatc atacaatccg aacttttata aggcaacaat tcagcgcaat
    13921 aataaaagag gagtttggta atattaatct cattgccggc gtggcaaccg cagggatacc
    13981 tcatggagta ttggtggcgg aaaatttagg actacctttt gcttacgtca gaagcagtaa
    14041 aaaagatcat gggctgggca atgaaattga agggataata gaaacagggc aaagcgtgat
    14101 tatgatcgaa gaccttatct cgaccggcgg tagcagcctt aatgcagtaa atgtagttaa
    14161 agaaaaagga gggatggtaa aaggattgtt agcaattttt tcttatggtt ttcaaagcgc
    14221 tgaagagaaa tttgtagcag ccgattgtaa aatgttcact ttaacggatt atccgtcact
    14281 tttagagttt gctctggaaa atgattatat tcgcaaaacc gaccttccga tacttaaaaa
    14341 atggagggaa gatcctgaaa attggtattt ggaaataaaa aactaaatca aaagtgaatt
    14401 ttatagctga caatataagt cttgatgttt caaaagagag tattgataat ttttttaagg
    14461 acataaatgc tcttaaactt attatgcccg ccggagtaga gcaatggcaa actgaagggg
    14521 ataaatgctc gttttttata aaaaatcttg gaaatttaaa aatgaaaagg agttttgata
    14581 ttcctgtttc agaatatgca tacagatcaa cagcagactc gaaagtggac tttactcttt
    14641 tatttcattt taacgaaaat ggacaaaaca aatcaacggg ctattttgag ttgcaagctc
    14701 aggtcaatcc atttatgaaa atgatgttgg agcgccctgt aacaaatttt gtaaatctac
    14761 ttacccagaa tctgcacaac cactttaaaa ataaatgttt ttcattttga gatatgaatt
    14821 aataatacaa tcataacata ttaatagtga atatattaag gtttcttctt tacccattcg
    14881 gcttgattta cggcatcttg ttgatgcttc gcaacaagat gtacgattgg ggtattttta
    14941 aagcaaccag ttttgacata cccataattt cggttggcaa cctttcggtt ggtggaacag
    15001 gtaaaacacc gcatgttgag tacctgatac gtttgttaag tgatgagtat aaaatagcag
    15061 tgcttagtcg gggttataaa cgcaaaacta aaggctttaa aattgccgat gacaattcaa
    15121 ccgtagctga tatcggagat gaagcgcttc agatcaacaa aaagtttaaa aatatcctgg
    15181 tagcagtaga tgaaaatagg gtgaatggta ttcgtaaaat caaaaaacag tatcctaaca
    15241 ttgaggttat catcctcgac gatgcttttc agcataggtc tgttaagcct ggtttgtcta
    15301 ttcttttaac cgattttcat aaactctata cttcagatta cattttaccg gttgggaaat
    15361 tacgcgagtt cagagcagga gcttcacgcg ctgacattat aatagttact aaaacaatga
    15421 ggcctctttc tccaattttg gccagagatt taaacgatac tataaaacca ttatcacatc
    15481 aacaattatt ctactcttat atcaattact taccgcttcg gccaacaacc ttaactgata
    15541 aaaatcagtt agcacataaa atcagttcgg tgttgatctt tgccggaatt gagaatattt
    15601 atccattgct cgatcacgtt tcaagaatgg taaattacat ggagtatttg aagttcagcg
    15661 atcatcactt atattcggaa agagacctga atctgataag agaaaaattt gataacctgc
    15721 tgggaaaaga taaaataatt ataactaccg aaaaagatat agttaggttg atctgccctc
    15781 aggtaccggc tcaacttgtt gatgtgcccc tattttattt acctattgaa ataaaaatac
    15841 ataacgaata cagcaaagaa ttcaataata aaattaaaga ttatgtacga aaaaatagaa
    15901 aagacacgac tattcattga gaatatgatt ggcataaagc cagaaatcgg tattgtatta
    15961 ggaactggtc ttggaaattt cacaaacaag atggaaatta taaaatctat tccttacaat
    16021 acaattccaa attttcctct ttccacagta cagggacatc ggggacaatt gattttaggt
    16081 aaaattggag gcaaaatctg tattgccctt caaggtagat ttcattatta tgagggttat
    16141 tccatgcaag aggtaacttt tccgatacgt gtaatagcaa ggcttggcat caaatatctg
    16201 ttcctttcta atgccagtgg cggaaccaat cccgactacg aagttggcga cataatgata
    16261 attgaagacc acatcaattt aacaggtcaa aacccattga taggaccaaa tgacgatcgt
    16321 ttaggtcctc gttttcccga catgagcgag gtatatgata aaaagctgat tgctttagct
    16381 aaatccattg caacagatta taagatcaca gtgcaatgtg gggtttatgc tgctgtaacc
    16441 gggcctaatt atgaaacgcc tgccgaatac cggtatatcc gaaaaattgg cgccgatgct
    16501 gttggtatgt ccacagtacc cgaagctata gtagcgcgcc atatgaattt gccctgcttt
    16561 gcaatatcag tcatttcaga ccttggcgtt gaaggtaaga tagaaaaaat tacacatgaa
    16621 gaggtaattg atgccgcaag tcttgccgag ccacttatga ccaaactgat taaaaaaatg
    16681 atacaagaat tatgattttg gtcaacatca tttaaaaagt tattatgaga aagagttaag
    16741 tgtaacaaaa tcggcaattt agaatatttc cttaattttg gcgccaattt tttaaaatta
    16801 ataataaaag aaaatagtaa aaaaattatg aaaagagacg ttcaggtatt tgatattatt
    16861 aagcgcgaac gcgaaagaca gatgcatggt attgagttga ttgcatcaga gaattttgtt
    16921 agcgatcagg ttctggaagc tatgggttcg gtaatgacta ataaatatgc cgaaggttat
    16981 ccagggaaac gttattatgg tggttgcgaa atggttgatc tgactgagca gttagctatt
    17041 gatcgtgcct gcgaactttt taatgcagag tatgctaatg tgcagccaca ttccggtgca
    17101 caggccaaca tggcagtatt tttagcttgt ctgaaaccgg gagatacttt tatgggactt
    17161 gacctttcgc acggtgggca tctgtcgcat ggctctccag taaactcctc cggtattctc
    17221 tacaatccaa tagcttatgg tgtttcaaaa gagaccggta ttattgatta tgatcaaatg
    17281 gaaaaaaccg ctaaagagca taaacctaaa ctgattgttg ctggcgcttc ggcctactca
    17341 cgcgactggg attttgctcg tatgagagcc attgccgatg aagtgggcgc actgctaatg
    17401 gctgatattg ctcatccagc cggactcatc gctcgaggag tattgaatga cccattacca
    17461 cattgccatt tcattaccac cacaacccac aaaacgttac gcggaccacg cggaggtatg
    17521 atacttatgg ggaaagattt tgaaaaccca tgggggctta caacgaaaaa aggagaggta
    17581 aagatgatgt cagacctgat caattcagct gtatttcccg gaatacaagg agggccgctt
    17641 gagcatgtta ttgcagcaaa agctgttgct tatggtgaag cgctatccga tgaatatatg
    17701 gagtatatag tacaagtaaa gaaaaatgcc gcagtaatgg ctaaagcttt tatcgaaaaa
    17761 ggatatcatg taatctccgg cggaacagat aaccatctgc tgcttattga tttacgcact
    17821 aaattccctg atattacagg aagaaaggtg gaaaatacac tcgtacaagc tgatattaca
    17881 gtaaataaaa atatggttcc ctttgatagc cgcagcccat tccaaacttc aggacttcgc
    17941 ttaggtacgc cagcaataac tacacgcggg ctgaaagagg aacacatgca accaattgtt
    18001 gactatattg ataaagttat ctccaatatt gacaacgaat cattaattag tgaagtgaaa
    18061 aaagaggtaa acaaaatgat gaataaattc ccattatttg cctactaatt ttttgctaac
    18121 tatctttatt acagaataat aaaaaaatat ataaatactt ttgaacttct ttttcaaaaa
    18181 gaggtttatt tcaaaaattt attaatccaa taacttaaca attaataatt tttatttatg
    18241 agaaagattt cattgttaat ccttctattt atcgggctta cagtttattc acaagatgat
    18301 gcgagccagc aagaagcgcc aagccgttgg aaaagaaacg gtatcgccag tctccagctc
    18361 aatcaggcaa gttttaataa ctggtcggct ggtggtgaga actcatacgc atttcttgca
    18421 ttaatgaagt attacgctga ttatgcacac gaaaactttt ctgttgacaa caaccttaac
    18481 ttaaaatatg gactacaaag tgtaggtaaa aatgctctac gaaaaaatga agaccttatt
    18541 gagtggaaca ctaaaattaa tcagaagttt tcgaaaaaat ggagcgctag cggccttatt
    18601 aattttacct cacaatttgc aaatggttac aattatccgg atgattcaac ggtcatttcc
    18661 aaatttttag cgccagcgta cctgactatc gcacccggta ttttatataa accggttgac
    18721 tatttcagta ttttgtttac acctgcctct ttacgtgcaa tctttgttac cgaccaagaa
    18781 ctggctgatc tgggagcctt tggcgttgat ccggcagaat ttaacgaaca aggtgcaaaa
    18841 gttactgacg ggaaaaatag taagattaaa cttggcgcct ttgctgagtt ttatcttaaa
    18901 aaaaatgtaa aaacagatct tgatctcgaa tcaaaattga acttttttta taactattta
    18961 cccgataatg ccttaccaag tggaaagatt ccattggata taaactggca aaactttgtt
    19021 aattaccgga tcagcaaatg gttttcgact acctttttta ttcatctggc atatatgcct
    19081 accgatatcc gtattgaatc catacctcca ccaagtggaa ttggcggcaa tgaattgaaa
    19141 gtaacgccga ataacaaggt tcaattgaaa cagacatttg gaattggaat agcttataat
    19201 ttttaacttt tcttaatcga actatatatt caaaactatg agtataataa aagaatttaa
    19261 agagtttgcc ttgcgtggca atgtaattga ccttgctgta gctgtggtta ttggcggggc
    19321 ttttggaaaa attgtttcat cttttgttgc cgatgtgtta atgcctccaa ttggcttgct
    19381 tttgggtggc gttaatttta ctgatttggt tattacccta aaaaaagctc aaggcgatgt
    19441 ggctgctgta actttacaat atggaaattt tatacaggca gttgtggact ttctaatcat
    19501 cgctcttgca attttcatga tgataaaagc aataaattta gcgaagaaaa aggaagaaga
    19561 agctccagcg cctccaccag aaccaacaaa ggaagagaca ctattaacag agatcagaga
    19621 tctgttaaaa aagtaatttt aattttggta aacgaaaaaa gccgccttga aaattatggc
    19681 ggctttttat atcgaatcac gagttgttca ataacttaca ctatcttctt tatactgaaa
    19741 tattcggaaa tcattatccc atcaatagtt attttattac aatttcatca gcaaaaatcc
    19801 aggcattatt accggctcct ttatgccaat cgggacaata ccctctgttt tttccaatga
    19861 tttttatata tcttgtggaa ataggagcaa attttaaagt gaaatcgtgc gattgtatgt
    19921 tccagtcatc ctcagatatt gggtttttaa catttccaat aaatctgaat tttttgccat
    19981 catccgaaat tgaatattcc acaaactccg gcataaaaat ccatgcattt acatcttgaa
    20041 aaaagccggt acttaaagtg ctgatcctca tagtttttcc taaatcaaca acagcttcga
    20101 gatttacacc ttcataacct tgccaacgtc cggtacggaa atcgttgcca ccgtgttgat
    20161 gatcaattag cgctatgttt ccccctgcgc tatactgcgg agcgaaagga tattttatag
    20221 taatagtcct cccaggctct attttgacaa atttagcctc aattataaag ctatcaattt
    20281 gatcatttct ctttgctttc gccttaatta tagtagtgga ttcaatagtg attgggttag
    20341 tatattggat gaatgtgttg ttttcatcat tcatgatctt atatttaatt tcagccagtg
    20401 ggtcggcgca agcaagctca atggtttgag tgtcaaaaaa gacgctgttc cctgatttta
    20461 tgaaaggaac aggggttatt agcttatcgg ttatatgtgt tattggtatt tgttcttctg
    20521 acgtccccca acttttattg ggcttatttc ccatttcgaa tatcaatagg cctccattca
    20581 ttatctgctg atgtgaaata taactgttag gccagatttt accgtttaat gttgcttttt
    20641 gaatgtattt gttctctttc gataagccct ttgccagtat tacaaaagtg tttccgtttt
    20701 ctaatgagat agtaatctta tccatcattg gcgtaccaat tgtgtaaaaa ggcgatcctg
    20761 gcgttaccgg ataaaagccc attgcactaa atacatacca agctgacatt tggccacaat
    20821 cttcgtttcc gcacaacccg tcgggatgat gagtataaag attatccata atcttccgaa
    20881 gaatttcttg tgttttccag gctttaccgg catagctgta aagatatgcc atgtgatgac
    20941 ttggttcgtt gccgtgagca tattgcccga tgaggcctgt aatgtcggct tgatgcctgc
    21001 cagtggtaac tgaactttcg gtaaacatct ggtcaaggcg ttgaacgaaa ttctctttcc
    21061 ctcccattaa ttccattaaa cctgaaatat cctgcggaac aaaaaagctg tactgccatg
    21121 agtttgcctc tgtataattg aagttaacct ctgcagggtc gaaaggggta aaacgggttt
    21181 cgttattttt ggcacgcatg aagccggttt ccgggtcata aatattttta tagtactgtg
    21241 cccgcatgat gaagtcatcg tatgtttcag tatcgcccat ttcttttgcc ataactgcta
    21301 tacaccagtc atcataggca tactcgagag tctttgacac cgattccggt tcagcatcag
    21361 aggggatata tcctttagtt ttataactat caagaccgta atggctttgt gtcgcacttt
    21421 taacacaggc ttgtagcgct aaattaacat cgaaattcct gatgtttttc atataagcat
    21481 caacaatgac aggcactgcg tgatatccaa tcatgcagtt ggtttcgttt gcagcaagct
    21541 cccaaacagg tagtaaacca ccttgcttgt gatgtaaaag cattgtgttt attaagtcgc
    21601 tggtaagttg ttgatgtgtg atggtcaata gcggatgtag cgctctgaag gtatcccaaa
    21661 gggaaaatac tgtataaatc tctctatcct gactaatatg tatcttcata tcgtgcccac
    21721 gatatgcccc atccgtatcg gtaaaaatgt taggcgctat catgctgtgg tatagcgatg
    21781 tgtaaaaaat tttctttaga tttttatcat tggattctgc ctttatttta ctaagttcca
    21841 tttcccattt ttgcgaagcc attgtttttg tttgttcaaa atcccaactt ggaatctctt
    21901 gcattcgatt cagctttgct ccttcggtat ttactgccga aatgcctact tttattaaaa
    21961 caatctcttc gtctttagtt tgaaaattta cccatgcttt aatgtttttc ccttgggcat
    22021 attgtaatcc ttcgtctatt ttgttatcta ccgaaatacc gtaaccggcg aaaggtttcg
    22081 aaaactcagc attaaaaaag acccattggt tatcagccca gcccttagtg cggcgtaagc
    22141 cctctatttc agtatcactt acaaacctta tccataaatc gaggatttga tctgaagtaa
    22201 caccttcgaa aagatcaatt ataatgtttg cattttcagt ttttgggaag gtgtatttgt
    22261 ggaaaccagc cctttgggtt gtggttaact ctacgttaat gttataatca ttaagtaaga
    22321 cctgataata gcctgggctg gcagtttcgg ttttatggct gaatttcgat tgataaccag
    22381 attcattatt ttcatcctta gtagggttca atattacttt tcccactgtc ggcataaacc
    22441 ttatatctcc ataatcacca acacctgtgc cgctcagatg tgtatggctg aagcccatta
    22501 tagtgctatc agagtaatga tatcctgaac atccgtccca atcatcttta cgcgtatcag
    22561 ggctcaattg aaccatccca aaaggtagcg tagccccggg aaaaacatgc ccatgagcat
    22621 cagtcccaat aaaaggatca acataagagg ttattgacaa atgattctct gaacacgaat
    22681 aggcaaaaag agcaattacg attatcaata aaaaccttgg agagaaaaca tgccaatttt
    22741 ttaaatctat tagtttggta aataatgatg cttgtttatt gttcgacgta acaatttgat
    22801 ctctactgtt atttaatgtt aacatcgtgt tttttgatac tttaacttgg tttgaaaaat
    22861 taaacttttt cattttgaag taattttaat taaaactttg aatatttaac taacgtattc
    22921 agcctatgtt cctgattttc tttgtgcaaa aaaagcttac tctccgacca atcagatata
    22981 attgtaacgt atttccttta tttattatca actcgaaatt gaataggcac ctcgctatcg
    23041 gtatcgctaa tgaatagaat gccaaaatcc tgaagtttct gttttaaggt ttaaaaattt
    23101 tcaagaattc cattatggat tagtgtcagc ttgttggctc gggagaggtg cggatgtgta
    23161 tatatttcgt ttggctctcc atgagttgtc catcgtgtgt gaattattat gctaataaag
    23221 acttcagtgt ttttgttggc gatataattt tctaagtcgt tagctttgcc ttttgtttta
    23281 taaacaataa ttttcttttc gagaatggtt aatcctgcac tatcatatcc cctgtattct
    23341 atctgtttag ggcatttaat aagaaatggg tgggcttgtt ttttcaaaca catccaacaa
    23401 ttccacacat aacttaaaat gttaattggt taatttggtg tattttattt gaagtttaat
    23461 acgtttgttc atcaaatttt catcgttcgg ccaagtgcca tgaaaagcga ctcgcgttgg
    23521 gcttaatgat gcgccggaaa tacccatagt gaaaacggta ttctcctttt cgccgagcat
    23581 aattttttga aggctttgcg ataatcggaa aaagtattct ccttttttct tatcatactg
    23641 accaccgaaa taacttaccc cctcaaattg atcttcaata aaaccaaggg tgccatcatc
    23701 ttttacgaaa tagtaaacca actcgtgagg aggcttgtaa atactattgg tatcaacatt
    23761 atgaaaaact aatttagcct cgttgattac tattttttga tctttgaacc aatcttttat
    23821 atagggaaat gaaattcggg tctccactcc ccccaacgat tgtagataca ctttctcttt
    23881 gcctaaatca tgatttccgt ttagttgagc tacaaagtca ggatcgccca tggaataatc
    23941 gtgggtaaag ttcatataac gggcacaagc agcatttatc aaaaaagagt acattttagc
    24001 gtcgccttca tcatttttat aatagaggtt aaggcatgag ttaatgtcaa gaagatcgaa
    24061 aaaggcaata actccattat tggctaccgg ttcaggtttt atatagagcc ctttcataaa
    24121 atcctgaaaa ttttcatttg actcttttac tgaagccggt gcattgagta tgttgtttcc
    24181 aaattcgtca tttaacctta tacgcaactg aggcttaact tttacggagt caatcattat
    24241 gctgtcgcgt gggctaaaat taatatccat gctggcaagt tcggtggatt ttgttgctac
    24301 aagagagtta gaatagtagg ttgaatcgta atacaatgat tcgtcaagtt caaacacttt
    24361 gattgtttgt gttgtagctg tatcgccata gtaataaacc ggctgaaaat taggtaagga
    24421 ataaaacgaa tactgcataa caaggactat ggagtcgagt tccgggtttt ccccaaaatt
    24481 catattaacc gtcgaaagcc ttacttgtgt agctatgctt attgtgttta atccaaaaat
    24541 aggatccaaa taacttccaa gcatactggt agcagtatta tcagttttta cgctatcggc
    24601 aaaaacagaa tgtacgagta cggtagttgt atcacttgaa aagagttcta atatttcgct
    24661 ccccggaatt aaacctattc ctactttatc gaaatcgttc ttacaatttg taaaaacaat
    24721 tactataatg ctgaaaatgg aaactaaaag gaaaaaatgg ttgttacgat tagaagatgt
    24781 tgatcctgag ttgaatttaa aattcatcaa tttcttttgt gtttaagaag tttctcgtaa
    24841 aaatcgttgt aaacagtaat ataattatca tcgcccagaa attcgagtaa cggcttttct
    24901 gtgctcctga ctaactcctc tagctcagga tttatttttt cacttccaaa aattaaagca
    24961 tcagaatatt taatagctgc ttttgaaatt ttgatataat cagggtttct ataattttta
    25021 aggtcaacct ctttaatttt atcaaatttt agttttttta ctaaagtatg gttaagcata
    25081 tcttcgaaaa aatcgtcgta caacgaatat attaccgttg aggaggaaaa aagcgggttg
    25141 tctttaaata ctctttttat ataaagtggc actaacgagg aaaaccaacc atgacagtgg
    25201 ataatatcag gtggccagcc gagttttctt actgtttcaa taacacctcg ggcataaaat
    25261 atcagtcttt cgtcattatc ttcaaaaaac tgcccgtttt tatcatgaaa tattcctttg
    25321 cgttgaaagt aatcttcgtt atcaatgaaa taaacctgca ttctggcttg ttgtattgat
    25381 gctactttta ttattagtgg gtggtcggca tcgtcaataa tcaggttcat tcctgatagt
    25441 cttatcactt cgtgtaactg attgcggcgc tcatttacct ggccatactt aggcataaaa
    25501 gttctgatct ccttaccgct ctcctgtgta ccttgaggca aatatcgccc gatattgctt
    25561 aaggggcttt cgtcaaggta aggagtaatt tcttgcatta catagagaac ttttatcttt
    25621 tctgctttcg gaatcataat gtaactgaat tgtaatctgc aaaagtatat aaaatatttt
    25681 aaacacttaa ccaaattatt ttcatagtcc ttggtaatta attaataatg tgtgttttat
    25741 ggtgtatttt ttactctttg tggatctttt gccttgttgt tgggagcttt aatacgaaga
    25801 aattgttttg ttctaaatgt tatttttgtc aactgttcgt tttccaattt tcattaaaca
    25861 tttgtaaaac ggtgataaaa aaagatatag attatagcat tcttgatgga aagcgttttt
    25921 attatagctt tttagctggt tcgcagcggt tattcgacaa tcaggttttg ctcaataaaa
    25981 tcaatgtttt tccggttaaa gatgctgata ctggaactaa ccttgtttct accgtacgtt
    26041 caatcattga ttccattatc ccctctgaaa attttgctgt tacagtaaat gctgtagctg
    26101 atgcagcttt ggtaggtgca agagggaact caggtattat ttttgcccag tttctttatg
    26161 gattcagcag cgaattcaag gatgaacaga caattgacat tactgcattc tctacggcta
    26221 tcaatcgtgg tgtacgccat tcttacgaag ctatttcaaa ccctgtggaa ggcactatga
    26281 taacagttat tagagaatgg gcagaatata ttcaaaagat aaaagataaa ttcaacaact
    26341 tcaatcagtt gatcactgaa tcttataacc aggccaaaca atcattaatc gagactcagg
    26401 aaaaacttga agtacttgct aaagctaaag ttgtggatgc aggagcaaag ggttttgttg
    26461 tttttcttga agggatgatt gactttttta ggcatggcga attgaaaaaa ataatcggtg
    26521 cacgtaattt agtaaaaaca acggctcttg aaggtgcaca tgatcacgaa aatatcactt
    26581 atcgttattg taccgaagcc atgctggtgg gagatgatat tgatcgagag atactccgta
    26641 gcaaaattga caaccttggc gactcattgg tcattgccgg gtcgcctaag aaaatgcgta
    26701 ttcatattca tactgatacg ccggacgtcc ttttttcgat tattaacaat tttggcaata
    26761 tcacttttca aaaagtggat gatatgatta tgcaaatgga tatagctgaa aatcgtaaat
    26821 atccaattgc tctggtaact gattccacct gcgatcttcc tcaggagttt gtcgaaaaac
    26881 atcaaatcat tatagttcct ttagctatac atgtgggaga tacttattat cttgaccgat
    26941 tgacaatgaa atcgaaaaag tttttcgaat tgcttaatac atttcctgtt tatccaagta
    27001 cagctcaacc ctcttttaag gattttagca acacttataa ttatttgagc actcatttta
    27061 attctatcat aggtattcat ctcagcgaaa ctatgagtgg cacctattcc aatagtcgca
    27121 aagctgcaaa gacaataagt aattttacaa aaaaacctat tagcgttatt aattctaaaa
    27181 agttgtctgg cgctttaggc ttaatactta tgcgtgctgt tgatgctttg caggataaat
    27241 ctattaccca tgatgaattg gtggcacaaa ttgaacaatg gtgtgataaa actgatattt
    27301 ttgttactac caaaaccata aaatatatgg ttaaaagtgg tagattaaat gctatgaaag
    27361 ggtttatagg taagttgctt agagttaagc ctattgtaac tgttaataat aagggtattc
    27421 cagagttgtt tgggaaacca ttaacagaaa aatcaagcag gaaaatggct gttcgcacta
    27481 tagaaaagaa aattaaaagt agaaagctat ggggatattc cattactcat gcaaataacc
    27541 catctacggc cgattggtat gccagagaga tagaaaaaat tataggttta aaaccttgtt
    27601 ttatacagga tggttcgccg gtgttaggtg tacacacagg cccagatgtt gtggctgtgt
    27661 cattgatgta tgagtaaaaa gtatttattc atacctgagt gattcaatag gatcgaggtt
    27721 tgcagcttta atagctggaa tgataccgga aagtaaagca acgccaaagc aaagcgaaat
    27781 accaattaat atccatgccc atggaataat aaagctactt tcagtaaata ttgttaccaa
    27841 attgccggca aggattccaa atatgattcc caaaattccc cccatttgtc ctatcaaaat
    27901 agattcaaca agaaattgat ttctaatgtt taaccgtgtg gcacccaacg actttcttat
    27961 cccgatttcg cgtgtacgct cggtaactga aaccaacata atgttcatca aaccaatggc
    28021 agcccctaaa agagttatca atccaatgat taatgcagca actttaatat ttttagtatt
    28081 ctcattaagc atttgcagta actgatcgct tttagaaatg tcaaagtcgt tctcttcgta
    28141 aagtttgttt tgtcttatta tcctaaaaag gccggtagct tcgcctatag catcatcaat
    28201 attagttatt tctgccggca ttacatttat gttgtaagtc atgttaggtc gagaaaaaaa
    28261 gtgccttgca tttgtaatgg ggataaatgc ttgacgatcg ggggtaaatc ccatgcctgt
    28321 tcctttttct tttataacac ctataacgcg atatctgcca ggccctacag tgatgaactt
    28381 atctaatggg ttctccttat tgacaaacag attttttgcc acttcaaaac caattactac
    28441 tacgttagtt cccatcgcca tttcatggtt gctaaatgcc cttccctttt gtaactcaag
    28501 gcccgaggtg gcgaaataat tttcgtctgt gccaataaca gctatattcg gatttgtggc
    28561 taatgaacga tttcggatag tagctcccca ggtcgcatat gtaaataccg atactgtggc
    28621 cggatattca tacgatttgc tgaaatccat tgcctgacgg tacctgatgg gttcataata
    28681 ttttgcttta tggatattag cgccaacctg tatttgcatg gatcggtttc tgatagtaaa
    28741 agtgttggat cccatcattt gcagattttc agcaaggcta ctttcaaggg cgtttattgc
    28801 ggttgatata ccgacaagtg ccatgattcc gaaagcgatg atcagcaccg tcaggatagt
    28861 gcgcagtata tgactgcgga tagcttccaa agaaatcttg atattttcaa tgaatatctg
    28921 aaattgcata ttgtttaaca cttattatga agcggttata accaactgat aacgaaagaa
    28981 agcgctattt gttggtggct ctatggcttt tcttttgaat gtttccatct tcgatgagac
    29041 cacaaccagt attcgggttg ttgcttgata gtattttcca acgttgaaac cactttcttc
    29101 gtgatttcat actcctgcgt tgctgcggga tcatcaaaaa gtggaatgaa ttcaacttta
    29161 taatgacctc tttttatttt ggtcatttgt gaatatacta cagcatggcg aaacttttta
    29221 gcaatttttt caaatccggt atagaatgga acttcgcgat gtaaaaactc tgtccggaaa
    29281 ggataatttc gcggtggtgt ctgatcagta agaaaccagg taaggatcac ctgtttgttt
    29341 tttagctgct ggataattac tcttaccacc tcgttcatca ctatcaattc aatttgagga
    29401 gcatatcttt ttctgatctt taatattaac ttgttaaatt gctcgttttg taatggctta
    29461 taaatagcaa gggctttatg tttaagtatt ctgggcgtgt ttgacatcca ttcccaatta
    29521 ccgtaatgcc ctgatactaa tacaacgctt tgccctctat caaaatattt atgcaaaatt
    29581 tccggattag gatatgttac tcgtcgttct atctcctgtg gactcatgtg aattgtttta
    29641 gctgactcga caaggatatc gcagaaatga cgaaaaaaaa gttttgaaag ggtgataact
    29701 tcacttttcg atttttccgg aaaagcgtgt tgcaaattat ctattacaac ctttttccga
    29761 taaccgatga tataatagat gataaaataa aacagattgg aaaaaagata aaggattctg
    29821 agaggcagta gggttaatag ccatatcagt acaaaagcga tataaaaccc aacaattttc
    29881 attattggta agtactatgg tttgacacca aaagccagat cgccggcatc cccaagccca
    29941 ggtactatat aagcttctgc tgtaagttca tcatcaatgg cgccaaccca aatagtaaca
    30001 tttttgctcg ataaatttcg ttttatataa tttactcctt caacactcgc aacgacagca
    30061 ataaggtgag agtgtctcgg cttttcatcg ctgattaact cacgataggt taaagccata
    30121 gaaacaccag ttgctaacat agaatcaacc aggattaaat ctttgtcttg aactgaaggc
    30181 ttggaaacgt attgcacatg tacatcgaag ttgccatctt tgtaatactt acgataagac
    30241 gaaatgaatg cattttccga tctgtcaaaa aaatcaagaa aaccttgatg aacgggtaat
    30301 ccagcccgaa ttattgaggc tactaccgga tagtttttta acaccttcat ctcagaagtc
    30361 ccaagtggag taataatttc acgggacttg tattcaagtt tcttgctgat ttcataggca
    30421 aaaattgaac cgatacgact gagatttttt ctgaaacgca tcgtatcttt ttggatttca
    30481 acatccctta attcggaaat atactggttg agcagcgaat ttccttttcc aagattattt
    30541 accatagggt tttcattttc ggcaaaagta attttttaat ttaagtatta gatattttaa
    30601 agtaattgac atctgtttta actttttaaa acatgccgtt gggttaaaat tttgaattta
    30661 ccaatggtaa aatgataaag gcctgatctt taaatttgaa taaatcacta aaaaactttt
    30721 ttgggttaga ttattgattt tagaaagccg atactgattt agtgtttatt tagtatgatt
    30781 tttactctcc ttgattattc acactcaatc agggtttaaa gagcgagttt gataccgggg
    30841 attgttttca attcggaata tagcttttgc attagatcgt tatcttttaa atcaacctgg
    30901 attgtaaagc taacatattt tccctcggcg ctatgcatcg ttcgccaatt agaattggga
    30961 atacggagct gcgaaaatat ggctcgcaaa taggcatttg actgctcaat gggatgtatg
    31021 gattcgataa ttattttaat gtcgaaggta actggaaaaa caagcttgtt atggccattg
    31081 cctccaatag ttttgaatga tgagaattgt ttcaatagag tttgtgtttg ttataagtga
    31141 tactttataa tagataacat ctaatccgac aaaatagttt cgattcgagc gccacatttt
    31201 tgcatagaat caataaattg aggatacgat tttgaaatac agccaacatc tttaactaac
    31261 aaggggcctt cagcaataag tcctgcgatc atcattgcca tcgcaatacg atggtcgtta
    31321 tgcgatgaga cctcgcctgg ttgtattttg cctggataga taattatttc gtttccattg
    31381 atgataatct caattccaag ttttgacatt tccaatttta gagctaaagc acggttactc
    31441 tctttataaa agagccgtga agcgcctgta attctgcatt ttcctgcaga ttggctggca
    31501 agaataacga gagcaggaac aagatcgggt gtgtgagtac aatcggcaac aaatgcaggt
    31561 atttcactct ttttcagata aagcttgtta tttgcacaca cggttttgat gccgatatct
    31621 tctaaaatag tgttcaattt cttatctgcc tgcaatgagt gtggcaggag tccggtaata
    31681 tcaatttctc cattcagcaa agcggctata ataggaaatg ccgctccact ccagtcgcct
    31741 tcgattatga aattttgagg gcagtatgct tgattaccgg atatgagata gaaatccgtc
    31801 ccctttgttt cgatatttac attaaaagct cttaatacct ctaatgtcat ttttagataa
    31861 ggcgcacttt ttagattatt aacaataagc tgtgaatcat tgtttaataa tggcaaagcc
    31921 atgagtaagc cggagatgaa ctgtgagctt tccgatccat ctaccaaata atagcccggt
    31981 tttaatcggc cttgaacaga tacgggtaaa aaaccattat tagatgtaaa cttaacgccg
    32041 ttagcttcca aagggtcgtt tacatttcct aaatgacgct tgagcaaagt accggatcca
    32101 gtgattgtaa ttttattttc tgaaagcgct gctatagcaa tcatgagcct tgccgtaagc
    32161 gctgactcat tacaatagat tatttcggga ttcgatttta atcctccctg aatttcgaga
    32221 ttttgattat taaacttgat ttcagctctg aaagaatggg ctatgttgag catcgccaat
    32281 gcatcgtcgc agcttgaagg attaattatt gttgattttc ccgccgctag cattgctgca
    32341 gcaattgccc taatggtgta gcttttggag ggaggagcat taacttcacc tttgacaaat
    32401 gatggatata caagagcatc cataataggc gattaacttc ctaaaaattt actctcttta
    32461 atatattcag ccagatcggc aaagccaatt tcttgaatta ttgctttccc taatttcgca
    32521 agcagtataa aataaatctg acctgcttgc cgctttttat cttttgctac ggcatcaata
    32581 atttccaagc gatttgatgg acagttgata gggagattca tcaacttcaa taatgattga
    32641 attctggcaa gatcatcgga acagatcaac ccttttttag ttgatatttc ggctgctgtt
    32701 aacattccaa tacttacggc tttcccatgg tttataccgg taactttttc gatggcatga
    32761 cctaaagtgt gtccaaaatt aagctttcta cgttcgccat tttctttttc atcttggttt
    32821 actatttcag ttttaatttt tacagaatga tggattagtt cgcctattgt ttgcaaatca
    32881 agagaaagga ttgcgttata attgttttca ataaaatcaa gcagtttttc ttcggcgatt
    32941 aaagcatgtt tgataacttc agaaattcca tctgcgagca actctttagg gagggtttgt
    33001 aaaacgacag gatcgcaaag cacgaagtcg ggttggttga tagtacctat ttgattttta
    33061 aagccgttgt aatttattcc atttttacca cctattgctg catctacttg tgcaagcagt
    33121 gttgtaggaa caaacccgaa acgaatacct ctgagataag tagatgctac aaaacctgca
    33181 atgtcgcata caatgccacc tcccactccc acaataaagc cactccgatc aacttcatac
    33241 tcaagcaacc gacgataaat ctcatttacg gtgtcaagag tttttatttt ttcaccaact
    33301 cctattgcaa tgtttggcca gttgctccag taccctccgt ataaatgatt tacattgaca
    33361 tcggtaataa ctacggtttc ggggggaaga tatcttgaga ggtttgaaat tttatcaccg
    33421 gtaataattt gggaaatttt accattgcta aaaatttcag tggactttat ggaattcatg
    33481 ttttatcttt ttttcaaaaa taatgaaata aaccctgata caataacaag tgtattggac
    33541 agacacaaaa tcagcattgg cttcataaaa taaacagttt aaccaaaagg gctgtttatg
    33601 aattaattca ggaaaaaaaa agccccaata gcatttgtta ttagggccta tggtcaaaaa
    33661 tgaaaatata ttattaattg tttttcacta ccttacctgt aatttgcagt atggtggggt
    33721 tttccgaagc gttagtttgt actgtgatag attttctaaa cgcaccattg ccactgccat
    33781 taaaggttac cgataattct gttgatttcc ctggaagaat aggctcttgt gagtatttta
    33841 gattagtaca tccacatgaa gctctggcgc tggttatcaa caagggctga tttccggtat
    33901 tggtgagagt aaaagaggca tcctttttta ccataaactg tacctctcca agatcaatgg
    33961 ttgtagccgc ccattcggcc tttggcccta tttcaacctc agtatctgta ctaacagttt
    34021 tcgactcttc tgattttgtc gtctttacag ggctttgtgc aaaaatgatt gtaacagaca
    34081 ttatcgtcag catcatcaag aaaattgact ttttcatttt aatctagttt taagtttgta
    34141 aaaacaataa attgatgaag caaaagtata ttataccaat ggaacagtat gttaatgaaa
    34201 ctcaaaactt tgttaatgaa atgtaaatat atcagtagtg tccactaaaa gttaaaaaac
    34261 atgattataa ttaactaagc agat
//
LOCUS       DAOOAI010000010        33429 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_1691_length_33429_cov_5.833613, whole genome shotgun sequence.
ACCESSION   DAOOAI010000010 DAOOAI010000000
VERSION     DAOOAI010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33429)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33429)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33429
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1691_length_33429_cov_5.833613"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="PK345_01550"
     CDS             <1..193
                     /locus_tag="PK345_01550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33739.1"
                     /translation="IELVARLYLSRLIGIHNETIFPPNQFSFAVLFEVMFDNFVRSVA
                     TIEGLNYYETKVKNFDWQK"
     gene            454..1707
                     /locus_tag="PK345_01555"
     CDS             454..1707
                     /locus_tag="PK345_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase U32 family protein"
                     /protein_id="HPX33740.1"
                     /translation="MTAKEIEIMAPVGSYESLMAAIQGGANSVYFGIGKLNMRSRSSQ
                     NFTLDDLAKISSICHQNGLKSYITLNTIVYDKELAEIKQIINSAKENNISAIIASDPS
                     VILYAHSQGVEVHMSTQTNITNIEAVKFWANYADVIVTARELTLKDVAAITKRIREEQ
                     ITGPSRNLVQIEIFVHGALCMAVSGKCYLSLDTLNSSANRGACLQPCRRRYKVADYDN
                     EVELEIDNEYIMSPKDLKTIHFLDHIIKAGVRVFKIEGRGRSADYVKVVSRCYREAVN
                     AYFNGTYNAENIAKWEEQLETVYNRGFWDGYYLGKKLGEWTHGYGSLATKRKIYIGKV
                     MNYFSKIGVAEIKLETHDLQIDDEIQIEGPTTGVLEDTVTEIRVDDQSVLKANKGESC
                     SLATKSIVRRMDKVYKVVNVKPSND"
     gene            1711..2586
                     /locus_tag="PK345_01560"
     CDS             1711..2586
                     /locus_tag="PK345_01560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HPX33741.1"
                     /translation="MALFNFHTHNHYCDGYFAPVIYVEEAIRQGFTDLGFSSHSPLPF
                     ENSFAIKNDEELVVYADEIRALKLAYQDKINIHLGLEIDFIPGVTKPFNHFKTIAGLE
                     YVIGGVHLINIKDHDDIWFIDGPRQETYDKGLKLLFGKDFQKAITAYWIQVREMISSQ
                     KPDIVAHLDKIKMHNKNRYFHEDESWYEAQLDDTLQLIKETETIVEVNTRGIYKGRSD
                     ELFPGIMALIKIHKLKIPITISSDAHRPEELSGFYPQTLEILKEIGFTELMIYSKSCW
                     SELPIKTIKGRGISG"
     gene            complement(2583..5147)
                     /locus_tag="PK345_01565"
     CDS             complement(2583..5147)
                     /locus_tag="PK345_01565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33742.1"
                     /translation="MAQREISEQSLSAEALERYKEDVARMISFLQFTFNTLGDADVPV
                     KEKDIIINQSWDKMFKNQRVQIEDDLDENRETPINKDVQAYLKDIDFFFNEVAFTFTI
                     QSIEHFINESGQHYFLVTFNRNLKGTLLNGNKIDSNKLRFAEINLDDSNKDLKIVSIY
                     TTKLNEKEELRRWWSELPVSWRIVLGEHLVVKDTLKMKHILWYNDSVAGVNMLIQRRI
                     NQGSFTLNTFDTLKIALSDNGIISAGLLDKQIQKIIEFDTINIASNKKIYSLEPLTKL
                     TQIKYLNFSNTAIINLMPVRNLSYLETMDGANTLVDDLDVIRYLNKLKELNLANTKIK
                     NLEPLSNFASLQRLNIANTQVFDLQPIALLNNLTDLNFSGTKISSLQSISSLTSLDRL
                     DFSATKVTDISLLKDLKSIVFLKFENTSVSDLSPLGYLKDLNLLFIDHTPVSDLTPLN
                     ALPNLTKVYCDQTQITRIEAVNFMEQNPGVLVVYETYELIKWWNDLPAEWKKIFSDKV
                     ANVTNPTKDELHDVTRLKILNIANNKDITSLQPLTNLTMLDQLYCQGSGINDLRPLMQ
                     LTELKDLNLSSTKVDDLTPLKNSKKLNRLLFDNTDVLSVLPLMELTELRTLYCDNSPV
                     IEDEIVEFLLKHPDCQVIYRTEELLSWWDGLPEIWKLIAADHITYSTQPNREQLHELT
                     NLRKLDLDANKDISNRLLEITNLDPLKNMILLESLIFSNTRVTSLSPLSGMSRLKSLV
                     CTNNPVESLQPLSNLLSLEFLDIQNTLVKQLALIGPLRNLKKLNCSGTQIKNLKGVEF
                     LENLEQIDCFNTKVKNLKPLQNLSKLRLIRCYNSNLSARAVAKFKALRPEVEIVFY"
     gene            complement(5257..6108)
                     /locus_tag="PK345_01570"
     CDS             complement(5257..6108)
                     /locus_tag="PK345_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33743.1"
                     /translation="MRKVKVVFFLLAFVVIQVYSQEPGVLLSRSGISAADEVSFYAET
                     KQVNQFIRRFNGEEDVRGVKLATNDPTYHSSDLRKKYLNMLFDHSNSDIPELLKNAFV
                     NDMVVDRDPKFLDFHGGDWFSEIEVLFQRGNEMVIFTLFMKLVKENLGSKWIINDVYS
                     PPYENLYKPNGTSASRFLHPLSHELDFMNLDKVFDSQEYIGDYFFQGFQVDKLSIFLY
                     EIRTNKLRFKQVTNVKFHFFQLKNWYMEISEFNRPGLNKGWLISNLIRLEDGQKDRII
                     DFIHHRE"
     gene            complement(6259..6828)
                     /locus_tag="PK345_01575"
     CDS             complement(6259..6828)
                     /locus_tag="PK345_01575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33744.1"
                     /translation="MKNKALIILFFLVISIPAFAQDNVSPQRKNAIDSLALEKVRDLG
                     KYISIIGDKKTPWSEANRVIDRALELFADGAMMGVSTLNSKEIKYWGIRTYLERLMAL
                     NYDKVTIEWYNIEYISDLEKQPDGRYVGVITIYQRFEGRVGDNLVYVDTTKKDITVYV
                     ERKETQIRGRLIGFWDVLLGDIRVSETMP"
     gene            complement(6835..7506)
                     /locus_tag="PK345_01580"
     CDS             complement(6835..7506)
                     /locus_tag="PK345_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33745.1"
                     /translation="MLLKIKNLISIRLVFLLIAAMIAVGFTGCQSKKKLARQQAAKEL
                     AEKIEKAKKDLLMVINDQGAMSISQKEKIVDDVKALNLNNEEVDALIIEAEKAIERHK
                     KLEAQRLEEERLRKEREAAEQRKIEEQKFDKMEDYFDAIASASTIDIANMRINDALKF
                     FASPEVPVLIIVYQDNEITDYDRPTTIKQYLEHLKDQKKNINRVHNIEYDTKGKIVEL
                     ELIKK"
     gene            complement(7776..8978)
                     /locus_tag="PK345_01585"
     CDS             complement(7776..8978)
                     /locus_tag="PK345_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPX33746.1"
                     /translation="MKINSFYVTLLLCFTLFFQSIDAQIETEKKGYVFTDEIRLNCTP
                     VKNQYRSGTCWSYAGISFIESELLKSGKGEFNLSEAFIVRQSYLEKAIQYVRWHGEKN
                     FAAGGASHDVLHIISKYGIVPEEVYTGLVTDDEMFVHSEMDLIFKNYVDAVIQNKNRK
                     LTPVWQKGFESLLDTYLGSYPEKFTYKGKEYSPRSFADALGLKLDDFIEVGSYTHHPY
                     YQKFIIEIPDNWLLGEIYNVKLDELLEIINTSLDKGHTLAWSADVSERGFSWKNGLAI
                     IPDETKPDLTGTEKERWEALTKEERDKMLFSFNEPIKEKLITQQTRQLGFDNYTTTDD
                     HLMEIIGRAKDQNGNTFYIIKNSWGTDGHIYDGFFYASESYLRYKTIYVMVNKNSIPS
                     KIADLLKL"
     gene            complement(9652..10323)
                     /locus_tag="PK345_01590"
     CDS             complement(9652..10323)
                     /locus_tag="PK345_01590"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX33747.1"
                     /translation="MNKNIKAIKVLLVDDHKIVRDGIISLLQGEENIQIVGQAENGLE
                     AIDKIGKLSPDLVLLDINMPILNGIECSQQIGKKFPKVKILVLTMLSEHEHIKNMLAS
                     GVCGYILKNSGRKELITAINTLMNGENYFSDEVKNVIIKELIRKKTNQGKISGEIIPL
                     TPRELDVLRYIIDEHTNQEIAEKLFISIRTVDAHRRNLLEKTGSRNTAGLVKFAIKNN
                     LLKNE"
     gene            complement(10327..12129)
                     /locus_tag="PK345_01595"
     CDS             complement(10327..12129)
                     /locus_tag="PK345_01595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPX33748.1"
                     /translation="MLGDNLGLWDYDLQTRKILVNNTWEKITGIVEPEKIIDFIKYTN
                     NIYPDDTSKVMASFEEMLQSNIETFDVEHRFLYQNKDWIWLHLIGIVAKRNENNQPCR
                     IIGTIQNITTKKQLELDLNYWLEVHRSFIKYANEGIFLYELEKPVVPTMPVEEQIDII
                     FNHGYIKTCNDSFANMYGYEHSSEMEGFTLSRLQGGDNFFYNKNFLKKLINSNYRVSN
                     EISIDKDRSGNPLYISNNIVGIIVNGELVRIWGSQFNNTDQILAQKELEKSELRYRLL
                     FKTNPVPLIIFSLDDFKLIDANNSAEKLFGYTHHELLNTDIKELRPEIADYTNKELLQ
                     KVKAELSKITEMTLIKKTKMKMITEVTTEIIDVQDKNYVIAAINDITLLRETEKMVIS
                     SLIEGEDRERTRVAKEIHDSLGQNLTAASLNFNAVKHLVENLNDKEKEKYNLGFSFLN
                     MAIEESRNIALNLMPKAIEDYGLILSLKSLFNKVEMSGDIKITFFENFKTDYRLPRNI
                     ELNLYRITQEVLNNVLKHAKANTIFIQLLLHKNEIIYTFEDDGKGFNIKSPTKEFKGM
                     GLVSISNRVKALSGSLEIDSTPKKGTAITIQIPL"
     gene            complement(12137..12781)
                     /locus_tag="PK345_01600"
     CDS             complement(12137..12781)
                     /locus_tag="PK345_01600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPX33749.1"
                     /translation="MRKLNEYNRPAVKVAFYFLIFGFFWILLSDKVLNYLVSDYNVRE
                     QMQTFKGWFFIVITALVIYFIVRTQIKMVVKLKNQLNESDYLYKQIVGLTHDVIWTLD
                     KNGVITFINQASKRVYGFYPEEMIGKKINSFTNQDSNEKNKKLYFDYLKQGITSIKLE
                     TQFTNPVGKTIYLQDNVHTLLDEHGNVSKIIGSSKDITSIKLSEKKTNRNKSTT"
     gene            complement(12827..15421)
                     /gene="mutS"
                     /locus_tag="PK345_01605"
     CDS             complement(12827..15421)
                     /gene="mutS"
                     /locus_tag="PK345_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HPX33750.1"
                     /translation="METPLMKQYSAIKAKYPDALLLFRVGDFYETFGDDAIKASNILG
                     IVLTRRANGAASFVELAGFPHHSIDTYLPKLVRAGLRVAICDQLEDPKLAKKIVKRGV
                     TELVTPGVSFNDKVLNHKENNFLASIHLSFKESGISFLDISTGEFYLTQGTNEYLDKL
                     IQSFKPSEIIIQKNKRNQFIEEFGENYYLTSFDDWVFTEDYARDLLLKHFQTNTLKGF
                     GVEDFNMGIIAAGASLHYLAETQHHQSANIRKISRIEEDKYVWLDKFTIRNLELLHSP
                     NENAATLIQVIDKTISPMGSRMMRKWITMPLKIKMSIEERLNVVEYLIDNPELSEIFR
                     ENIKLIGDIERLISKVAVGRVNPREVIQLVRGLHAIEKIKDGCAVTNNDPLKKIADQL
                     NPCHVIRNKTEAELEADPPVSINKGHVFRQGVSEELDELRDLLYSGKEYLEKIRKTEA
                     ERTGIVSLKIGFNNVFGYYLEVTNAHKDKVPQEWERRQTLVNSERYITQELKEYEEKI
                     LGAEEKILEIETQLFNNLVLSLADYTEPIQLNSLLIARLDCLLSFATVAIKNNYIKPQ
                     INDSFIIDIKNGRHPVIENQMPPGEAYIPNNVYLDNKKQQIVIITGPNMSGKSALLRQ
                     TALIVLMSQMGCFVPASSASIGLVDKVFTRVGASDNISSGESTFMVEMNETASILNNI
                     SNRSLILLDEIGRGTSTYDGISIAWAITAYLHNHPAFRPKVMFATHYHELNEMATTMK
                     RIKNYHVSVKEINNKVIFLRKLSPGGTEHSFGIHVAKIAGMPTEVIEHAEKMLKVLEK
                     SHSQDELNKKLNLPHTDNDYQLSFIQLDDPLLLQIKDEIININIETLTPVEALMKLNH
                     IKNMLIKK"
     gene            15757..15830
                     /locus_tag="PK345_01610"
     tRNA            15757..15830
                     /locus_tag="PK345_01610"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15791..15793,aa:Pro,seq:ggg)
     gene            complement(15887..16072)
                     /locus_tag="PK345_01615"
     CDS             complement(15887..16072)
                     /locus_tag="PK345_01615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33751.1"
                     /translation="MAGILDTDRYTAFLAEALDISPKEIHALLIIKNAFSLFMHDSAE
                     HFRNMVKVLDDMSLFLH"
     gene            complement(16109..16441)
                     /locus_tag="PK345_01620"
     CDS             complement(16109..16441)
                     /locus_tag="PK345_01620"
                     /inference="COORDINATES: protein motif:HMM:NF012286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33752.1"
                     /translation="MTLVGAGNVGATCANVIVHKDLAREVVLVDIKEGLAEGKALDIW
                     QTSVINNFNTRVAGVTNNYLHTKGSGIVVITSGVLQKPGMNRDNLVRSNTIIIIVSNP
                     LDVITYIV"
     gene            complement(16593..17183)
                     /locus_tag="PK345_01625"
     CDS             complement(16593..17183)
                     /locus_tag="PK345_01625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HPX33753.1"
                     /translation="MEKKVLNLFASAYFLGLLTAIPMILVLYFVYSYWLTHVQSLRRI
                     VFYSFVLIGFLSELFKFLLLRFYYLPKKSFTKPFDGILFSVMIAMGYATAANVYFFYE
                     WRYTDGIRIVLYTLPVAVFFISIIMGFFIGLSKFRSNNFDSLTGFSAAVILFGFYSFC
                     LLSGDYLLLSLIGGGVILIAFLLAAKSLNSKTNALL"
     gene            17337..17606
                     /locus_tag="PK345_01630"
     CDS             17337..17606
                     /locus_tag="PK345_01630"
                     /inference="COORDINATES: protein motif:HMM:NF017690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HPX33754.1"
                     /translation="MKNITIKKLTDEEIDKNAIKSWPIWEKEVSKFPWFYEATEDCLI
                     IEGIIEVEANGKIYQLKPGDFVTFQKGLKCTWDIKKPVRKYYNFR"
     gene            17646..18584
                     /gene="rsgA"
                     /locus_tag="PK345_01635"
     CDS             17646..18584
                     /gene="rsgA"
                     /locus_tag="PK345_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HPX33755.1"
                     /translation="MKGVVIKSTGSWFTVLDENGRQINCKLKGRFRIKGIKTTNPIAV
                     GDKVEFDIAEDEKIGLITKILPRHNHIIRKSTKLSKVSHIIAANIDLALLIVTIKEPE
                     ISPGLIDRFLVTAEAYHITSAIVFNKIDIYGDVDFDKLQKYEAIYRAAGYQTFRVSAL
                     TGENIDMLKEVIRDKICLFSGASGTGKSALINVIEPGLKLKVGDISTYHLKGRHTTTF
                     PEMHKLTGGGFIIDTPGIREFGLVEFRKEEIAERFPEMRAFMHDCQFNNCTHIHEPRC
                     AVKAAVENGKIAASRYESYLRIYNDDYLDKELWEWE"
     gene            18675..19340
                     /locus_tag="PK345_01640"
     CDS             18675..19340
                     /locus_tag="PK345_01640"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HPX33756.1"
                     /translation="MIIRNLTYDFFQPLKKSDKVSSALRLMYEYKISHAPVVEESTYL
                     GIISENDLLVMPNKSEKIESLCKALPKPFIKENEHILNALNIISNLQIEVLPVLDNDH
                     QYKGLITAEQVLKEIAGIFSLENPGGIIILEVNLNDYSMSEIARIIEANDTKILSSGV
                     KIIPDSTKIEITLKLNVMNIEPVIQTFMRYNYNIIFYFGDNEKNEEVLKERYELLMRY
                     LNT"
     gene            19375..20844
                     /locus_tag="PK345_01645"
     CDS             19375..20844
                     /locus_tag="PK345_01645"
                     /inference="COORDINATES: protein motif:HMM:NF018899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPX33757.1"
                     /translation="MKYLLLFLIIIFPFAQSATPKQLSSIKSEIPIDSIIEAENKTIS
                     ESSFVIDSIILTGNKKTKDFLIFRELTFKVGDTLNHNQLQENYQKSRNNLIKLSLFNF
                     VDFTDSLVAAGQYAHLLVQISFIERWYLWPFPIFEISDRNLNVWLKEKNFNRISYGIY
                     LIKHNNRGRGELLRLYLRSGYDERYELSYQVPYFNHKQTLGATLGAGWLQKHEVPYRT
                     IANKQEFIKDDDHYLFKQFYNFINFTYRPDIHQFHNFQIRYNFFYFDDTLLKLNPKYS
                     FKGLKTNEYMTFAYKFISDHRDSKINPLTGHYFEGQISKSGFGLLKDGYIEMMDLTGS
                     YRKYWEFPKRFYFSTDWTGKLSTNREQPYFYQRGFGYERNFVRGYELYVIDGQSFILG
                     KNTLRYALVPERKTTIGFLKSEKFSKIHYALFTNLFLDAGYVDSFRFYDPNDLSNHLI
                     YGVGLGIDLVTYYDMIFRMELSVNKQGEPGFYIHFKDTL"
     gene            20990..21874
                     /locus_tag="PK345_01650"
     CDS             20990..21874
                     /locus_tag="PK345_01650"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF002521.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HPX33758.1"
                     /translation="MLKKIAIFGKSIEKEGIEYLQNLLNRLEFFNCTLSIYEPFYKKI
                     ENQVRFPSNLKFFNTHEELKDNAEILLSIGGDGTLLDTITLIRNSGINVLGINLGRLG
                     FLSSISKDEILPAIDKVLSGNFVLEQRTLLELSKPEAIFGDFNFALNELSITKSSPHA
                     LAVINVFVNDSFLNKYWADGLLVATPTGSTAYSLSCSGPIITPESENFVITPIATHNL
                     TVRPIVIPDKCTIRITVEGRNQNYQVGLDSRFQSLRMTDEIIIKKANYKINLIQLHEK
                     DFFNTIREKLLWGKDQRN"
     gene            21975..22631
                     /locus_tag="PK345_01655"
     CDS             21975..22631
                     /locus_tag="PK345_01655"
                     /inference="COORDINATES: protein motif:HMM:NF027718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5011 domain-containing protein"
                     /protein_id="HPX33759.1"
                     /translation="MKHFLKLFIYMAALSLVFLSCNKIYEDKIPPTIILQGPNPYRLF
                     TSCSYSEPGYIIKDDLTPNSQIKVDIYDSTLNTTIPGDYYIDYYSTDLDGNTSYVRRK
                     IETRKMTLDYFNSGFIAYDTLKPMNIKTQYSIKSVLYSQEFKWIRIENFNNFGKQFKV
                     IMIPDTLGNLTLNYNFNDTIVFGTGNVYCNMTGFRLEYQIDVPENDIEMHYVTYRFLN
                     "
     gene            22649..23467
                     /locus_tag="PK345_01660"
     CDS             22649..23467
                     /locus_tag="PK345_01660"
                     /inference="COORDINATES: protein motif:HMM:NF039345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6089 family protein"
                     /protein_id="HPX33760.1"
                     /translation="MKIIKHCLIVLLAFLVKIIYPQDIDIGIFGGGSYYLGELNPGRQ
                     FLMTNPAYGGLIRFNLNDRMAFRGELIHGVVSGDDAVSKENELRNLRFTSTINELSVL
                     FEFNYLEYFTGSQIHYFSPFLYCGPALFTFNPKAKFRNEWILLRDIGTEGQMINENDN
                     RYNLISFAFAFGIGFRYSFSHRLGFTAEWGMRKTFTDYLDDISKNYYVDFNNLNPDDI
                     SLEKMLSDPSPIKHQPGMQRGNPQNNDWYSFVGITLTYRFTIGEKTTCVEFQNR"
     gene            23550..24311
                     /locus_tag="PK345_01665"
     CDS             23550..24311
                     /locus_tag="PK345_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008997927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HPX33761.1"
                     /translation="MGFKDKIDMKRLPRHIAIIMDGNGRWAKQRGLVRTVGHENGIVA
                     VREVAEAAAELGIEYLTLYAFSVENWKRPQREVEILMKLLLKTIDSEMKTLKENDIRL
                     LAIGNLISLQKKTYERLLWAIEETKNHKRMNLVLALSYSSHYEIVEAVKEIARKAKQN
                     ELSPEDINESVISNHLFTKGFPDPELLIRTSGEFRISNFLLWQLAYSEIYITPILWPD
                     FRKENFYEAIVNFQKRERRFGKTSEQVSENANKMK"
     gene            24308..26974
                     /locus_tag="PK345_01670"
     CDS             24308..26974
                     /locus_tag="PK345_01670"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HPX33762.1"
                     /translation="MRKIASLIRNNYILPANYLLLLVFLLNVPHFSFTQINIGPPELD
                     IDYMNPVEYEIGGITVTGIEFLDRNVLIMISGLNVGQKVKIPSDKFSDAIENLWKQGL
                     FEHVSITATDVQDKMVFLNIDLREKPRLSKYTFEGVKKSEGDNLKDEIKISSGDVVTE
                     NLITNSRNRIVNYYAKKGFFDAEVDIRQISDTTRLNSVILIFDIKKNNRIRIYDIEIT
                     GNKKLSDQQVLRALKNTKEKGAFRPFDEIETLIWDVTRHALLLQFDTLYAKAGEYLNN
                     NMKLRIFKTSKYIESEYENDKQNLIEKYNSLGYRDAKIVRDSIYRNDDMTMNILIEVD
                     EGPQYFFGNISWVGNTIYTDEFLSDVLKIQRGDVYNKEMLTTNLSFNPSGVDVSSLYL
                     DDGYLFFQAIPVEIRVENDTIDLEIRIREGKQARISKVNVKGNTKTNDQVVVRELRSR
                     PGQLFNRSNIIRTTRELAQLRYFDAETIKPDIQPNPADGTVDIIYEVEETSADQIELS
                     GGWGYGRILGTLGLSFNNFSLQNLFKKEAWRPIPSGDGQKLSIRLQSYGRGYISYNFS
                     FTEPWFGGKKPNSFSVSYFHSLYSNGLSKKDPSRSAFLTDGVVLGLGKRLQWPDDYFT
                     LYQAASFQIYKLDNYARIFSFGTGNGNYNNFNYNFIFARNSIDAPIYPRSGSEVSLSV
                     AATPPYSAFNNKDYKNMPVEEKYKWIEYHKWKIRTNFYMNIIQNLVLSTRVQFGFLGT
                     YNHDIGVTPFERFFLGGDGLSGYNNLDGRELIGMRGYSNESITPNYYLKDNVGGVIYS
                     KYTFEVRYPFSLNPNATIYALSFLEAGNSWESFAAFNPYTVFRSAGFGLRVFLPMFGI
                     LGLDWGYGFDKVPGIPDANRGHFHFSINQSID"
     gene            27053..27604
                     /locus_tag="PK345_01675"
     CDS             27053..27604
                     /locus_tag="PK345_01675"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPX33763.1"
                     /translation="MKTLKITIRVLIITITLSLTGFTNTFAQKFAYVDSDYILKSIPE
                     YQNAQNEIDELSKTWQAEIEAMFTQVEQMYKAYQADAVLLPEDLKKKREDEIIKTERE
                     AKELQRKRFGQDGDLFKKRQELIKPIQEKIFNAIEEIATKQNYAFIFDKASGPTIMYI
                     NARYNISDEVLDQIGSIMGVRKQ"
     gene            27711..28226
                     /locus_tag="PK345_01680"
     CDS             27711..28226
                     /locus_tag="PK345_01680"
                     /inference="COORDINATES: protein motif:HMM:NF015871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpH family outer membrane protein"
                     /protein_id="HPX33764.1"
                     /translation="MNKTVKLIALVAFIVFASFNSVQAQQKFGHVNFAQVYGLMPGQD
                     SIKKVYETYAKGLQNQLATMQAELESKYTDYQANQATWSNIIRQTKEKEIQDMQIRLE
                     DFNSQAQIDLRNKEDELTAPLIERARKAVEDVAKENGYTYVFNSTEGLLLYATPSDDI
                     MSLVKKKLGLK"
     gene            complement(28259..29056)
                     /locus_tag="PK345_01685"
     CDS             complement(28259..29056)
                     /locus_tag="PK345_01685"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPX33765.1"
                     /translation="MKKIAFKNKAIYYKLDGDGYPLVILHGFMGSSMAWNYFASQLKE
                     TFKVIRVDLPGHGKTPTIDKVHTMELMAEAVKTVLDHLNVKKCLMIGHSMGGYVTLEY
                     AKQYPEMLKGIVLFHSHAAADTEESKENRRRNIAIVKLNKKGFVKQFIPDLFAEINVV
                     KLHDEIEMLWNIADKTSAQGIVAALYGMKERTGKLDLLLNINIPVLFIAGKEDSRIPV
                     QNVLAQAILPRHSEVLVLADVGHMGFLEAPGETLRMIHSFASKVFNE"
     gene            complement(29064..30365)
                     /locus_tag="PK345_01690"
     CDS             complement(29064..30365)
                     /locus_tag="PK345_01690"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HPX33766.1"
                     /translation="MRYQPIDKSFFEANRERFGKLMKPSSVAIFNSNDEFIRSGDQAY
                     VYRQNSDFFYLTGINQEKSVLVIAPNHPDIKLRQILFLVETDEQTAIRDGDRLTREKA
                     MQISGIANVKWLSDLELTLRDLIIWSDHVYLNIYEYPKFSTEIDSRDHRFARQLQYQY
                     PAHNYERAAPLLTKLRMVKSETEINLIRKAIEITNKSFYRILKFVGPGVHEFEIQAEM
                     EHEFLINRSTGSAFPPIIAAGKNACVLHYNDNNDIIDDGQLLLIDFGAEYANYAADIT
                     RTIPVNGKFKPRQRQLYDLVLKVQQRAIKQMIVGNTMEKYNTFVNNEMEREIIKIGLL
                     NEEEVKKQDPDNPLFKKYFMHGTAHHLGLDVHDVICKYEPFKPGMILTCEPGIYIRSE
                     AIGIRLENNILITENGPVNLSADIPIKPEIIESLMSRDKEN"
     gene            30517..31209
                     /locus_tag="PK345_01695"
     CDS             30517..31209
                     /locus_tag="PK345_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b subunit"
                     /protein_id="HPX33767.1"
                     /translation="MGNSTFHLASITKKVIMALVGLFLVMFLLVHLGLNLTVLILEGD
                     REIFNKAAHFMGTNLIVKIVEVGLFGFLIVHIIIGIVLQLQNWGARPKRYKKRATTQL
                     SFFSRYMIHTGALIFIFLIIHFSDFYFKAKFTDLVPLITYDGKEYHDLGLLTLQKFKM
                     GGFVIGYIIAFILLGFHLNHAFQSAFQSMGWNHSRYMPFIKKVGLVISILIPLGFTII
                     PLVIYFSEAYSV"
     gene            31231..33150
                     /locus_tag="PK345_01700"
     CDS             31231..33150
                     /locus_tag="PK345_01700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase/succinate dehydrogenase
                     flavoprotein subunit"
                     /protein_id="HPX33768.1"
                     /translation="MIKFDAKIPEGPLTEKWSIHKRSVKLVNPANKTKLDIILVGTGL
                     AGAAAASSLGELGYNVKAFCFQDTPRRAHSVAAQGGINAAKNYRNDGDSVYRLFYDVI
                     KGGDFRAREANVYRAAEVSNQTIDQLVAAGVPFAREYSGYLDNRSFGGVQVSRTFYAK
                     GQTGQQCLLGAYSGMMRQVAKGKVTMYSRRDMLDLVVIDGKARGIITRNLVTGEIERH
                     AGHCVVLATGGYGTIYYLSTLAVGSNVSAAWAAHKKGALFANPSFIQIHPTSIPQLND
                     YQAKLTLMSESLRNDGRIWVPKKKGDARAANDIPEDERDYYLERRYPAFGNLVPRDVA
                     SRAAKERCDAGYGVGKTGLSVYLDFKDAIKKQGKKAIENKYGNLFELYYKITGDNPYE
                     VPMRIYPAGHYTMGGLWVDYNLMTTIEGLYAIGEANFSDHGANRLGAGSLMQCSGEGY
                     FILPVTIGDYLADEIKTPRILTDHPEFEKAEKEVKEKIDKLMAINGKQTPTTFHKRLG
                     KIMWDYCGMARNEKGLQKAMIMVQELEEEFWKDLKVSDSAKELNQELEKAGRVVDFFG
                     IAKLMIQDALNRKESCGAHFREESQTPDGEAKRIDSEYSYVAIWEYLGEDKPHTLHKE
                     PLVFENVELKERSYK"
     gene            33175..>33429
                     /locus_tag="PK345_01705"
     CDS             33175..>33429
                     /locus_tag="PK345_01705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HPX33769.1"
                     /translation="MNITLEIWRQKDAKSKGKFVIYKLNDVSEEMSFLEMLDFLNNTL
                     ISKGESPVAFDHDCREGICGSCGLYINGWAHGPAHLTTTCE"
BASE COUNT        10742 a         5891 c         6059 g        10737 t
ORIGIN      
        1 catcgaattg gttgcacggc tttacctcag cagattgata ggtatccaca acgaaactat
       61 ctttccacct aatcagttta gctttgcagt attattcgaa gtaatgttcg ataattttgt
      121 aagaagcgtt gcaactatcg aggggctaaa ttattatgaa acaaaggtta aaaattttga
      181 ttggcaaaaa taactggatg tgtactcttt attcaactac ctctttcttg aatatttttt
      241 tcctaaagat tggtttatga attagaacct gatagacatt ggagtataag caggttagcg
      301 ccttaatctc agctttggtg tttataatca gcattataaa agttacccgg aaaatttatt
      361 aagccgggat tggttttgtt aaatccgata ttcagcttaa aagggcttga tattgattaa
      421 ttcgagttat ttttgactaa tattttaata tctatgacag caaaagagat tgaaattatg
      481 gcgccggtcg gttcgtacga atcgttaatg gcggctattc aggggggagc caactctgta
      541 tattttggaa taggaaagct caacatgaga tcacggtcgt cacaaaattt tactcttgac
      601 gatctggcaa aaatttcttc tatatgtcat caaaatggtc taaaaagcta cattactcta
      661 aatacaatcg tatatgataa agagctcgcc gaaataaaac aaataataaa ctcggctaag
      721 gagaataaca tatcagccat tattgcctcc gatccgtcag ttatactata tgcacactca
      781 cagggtgttg aggttcacat gtccacacaa accaatatta ctaacataga agcagtaaaa
      841 ttctgggcaa attatgccga cgttattgta actgctcgag aattaacttt gaaggatgtt
      901 gcagcaataa ctaaaagaat ccgtgaagaa cagattacag gcccctctcg taatttagtt
      961 cagattgaga tttttgttca tggagccctt tgtatggcgg tatcaggtaa gtgttatctc
     1021 agcctcgata ctttaaattc atcggcaaat cgtggcgctt gcctacagcc atgcagacgc
     1081 agatataaag ttgccgacta cgacaatgag gtggagcttg aaattgataa tgagtacata
     1141 atgtctccta aagatctgaa aacaatacac tttcttgatc atatcataaa agcaggagta
     1201 agagttttta aaattgaagg tcgcggtcgt tcagcagatt acgtgaaagt ggtaagcagg
     1261 tgttatcgcg aagctgtaaa tgcatacttt aatggaactt ataatgccga aaatattgca
     1321 aaatgggaag agcaactgga aactgtttac aatcgcggtt tttgggatgg atattatctc
     1381 ggtaaaaaat taggtgaatg gacgcacggc tatggctcgt tggctacaaa aagaaagatt
     1441 tatattggaa aggtgatgaa ttatttcagt aaaataggag ttgcagagat aaagttagaa
     1501 acccatgatt tgcagataga tgatgaaatt cagattgaag gaccaacaac aggggtgttg
     1561 gaagatacag taactgaaat aagagtagat gatcaatcag ttttaaaagc aaataaagga
     1621 gagtcttgtt cattagcaac aaaaagtatt gtccgccgga tggataaagt ttacaaagta
     1681 gttaatgtta aaccgtcaaa tgattgataa atggctcttt ttaattttca tacccataac
     1741 cattattgcg atggctattt tgctccagta atttatgttg aggaggcaat ccgacaagga
     1801 tttactgact tgggcttttc tagtcattct cccttgccat tcgaaaactc ttttgcaatt
     1861 aaaaatgatg aagagcttgt ggtgtatgcc gatgagattc gagcattaaa attagcatat
     1921 caggataaga tcaatattca tcttggcctt gaaatagatt ttattcctgg agttacaaag
     1981 ccttttaatc attttaaaac tattgccgga cttgaatatg ttatcggtgg tgtgcatctt
     2041 ataaatataa aagatcatga tgatatctgg ttcattgatg ggccgaggca agagacttat
     2101 gacaagggac tgaaacttct gtttggtaaa gattttcaaa aagcaatcac agcatattgg
     2161 atacaggtaa gagaaatgat ttcttcgcag aagcctgata ttgttgcaca tcttgataaa
     2221 ataaaaatgc ataacaaaaa caggtatttt cacgaagatg aatcgtggta tgaagcccag
     2281 cttgatgata ctttgcagct tatcaaagag acagaaacaa tagttgaagt aaatacacgc
     2341 ggtatttata aaggacgttc tgatgagttg ttcccaggca ttatggcatt aataaaaata
     2401 cataaattga aaatcccgat taccataagt tctgatgcac ataggccaga ggaattatct
     2461 ggattttatc ctcagactct tgaaattttg aaagaaatcg gatttacaga actgatgatc
     2521 tattccaaaa gttgctggag tgaacttcca attaaaacga taaaggggcg aggtattagc
     2581 ggctaataaa aaacgatctc tacctctgga cgtaaagctt taaatttcgc cacagccctt
     2641 gcagaaaggt ttgaattata acatcttatc agtcggagtt tggatagatt ctgtagcggc
     2701 tttaggtttt ttacttttgt attaaaacag tcaatttgtt caagattttc gagaaactca
     2761 acgcctttca aatttttaat ctgtgtgcct gaacaattga gcttttttaa attgcgcaga
     2821 ggtcctatta gcgctaattg cttgacaagt gtattttgaa tatcgagaaa ttcaagactt
     2881 aataaattac ttaacggctg aagcgattct acagggttat tagtgcaaac taaagattta
     2941 agcctgctca ttccacttag tgggcttaat gaggttaccc ttgtgtttga aaagatcagg
     3001 ctttctaata aaatcatatt tttcaaagga tcaaggtttg tgatttcaag caggcgattg
     3061 gaaatatcct tgtttgcatc aagatcgagc ttcctaaggt tagttaattc atgaagctgt
     3121 tcacggttgg gttgagtgct gtaagtaata tgatccgcag caataagttt ccatatttca
     3181 ggcaaaccat cccaccatga taataactct tcagttcggt agataacctg acaatcagga
     3241 tgctttaaaa ggaattcaac aatttcatcc tcaattaccg gcgaattgtc acaatataaa
     3301 gttcgtagtt cagtaagctc catcaatggt aaaaccgata atacatcagt gttgtcaaat
     3361 aataaacggt ttaatttttt actgttttta agcggtgtaa ggtcgtcgac ttttgtagat
     3421 gataaattca aatcttttag ttcagtaagt tgcattaacg gtcttaaatc gttaataccg
     3481 ctgccctgac aataaagctg atcgagcatt gtaaggttgg tcaacggttg tagcgaagtg
     3541 atatctttgt tgttggcaat attgagaatt ttcaaccttg ttacatcatg taattcatct
     3601 tttgtagggt ttgtaacatt ggcaacttta tcagaaaaaa tctttttcca ttctgccggc
     3661 aaatcattcc accacttgat caattcataa gtttcgtaaa cgactaatac tccaggattc
     3721 tgttccatga agttcactgc ttcaatacgt gtgatttgag tttgatcaca atagactttg
     3781 gtgagatttg gcaatgcatt caatggtgtt aaatctgaca ccggtgtatg atctataaat
     3841 agtagattta aatccttgag ataacccaat ggcgaaagat cacttaccga agtgttttcg
     3901 aattttaaaa aaacaatact cttcaagtcc tttaataggc tgatatccgt aacttttgtc
     3961 gctgaaaaat caagcctatc caatgatgtt aaggatgata tgctttgtaa tgaactgatt
     4021 ttagttcccg aaaagttaag atcggtcaaa ttatttaaca gtgcaatcgg ttgcagatca
     4081 aaaacctgag tgtttgcaat attaagtctt tgcaaacttg caaaatttga caatggctcc
     4141 aaatttttaa tttttgtgtt ggccagattt aattccttta gtttgttaag ataacgtatt
     4201 acatccagat catcaaccaa agtattcgcc ccatccattg tttcgagata agatagatta
     4261 cgtaccggca taaggttaat aatggcggta ttactgaaat ttaaatactt gatctgtgtt
     4321 aatttggtca aaggctcaag cgaatagatc tttttattgc ttgcaatatt gatggtatca
     4381 aactctatta ttttctgaat ttgtttgtca agcaagcccg cagatattat accgttatct
     4441 gaaagtgcaa tttttaaggt atcgaaagtg tttaaagtaa atgatccttg gttaatacgc
     4501 cgttgaataa gcatgttcac acctgctacg ctatcgttgt accagagtat atgcttcatt
     4561 ttcaaagtat cttttacaac taaatgctca ccaagaacaa ttcgccaact aacaggcagt
     4621 tcactccacc aacgacgcag ctcctctttt tcgttgagtt tagtagtata gatacttact
     4681 attttcaaat ccttattgct gtcgtcgagg tttatctcag caaatctcaa tttgtttgaa
     4741 tctattttat ttccatttag caatgttccc tttaagttac gattaaaagt aactagaaaa
     4801 taatgctgac cagattcatt gataaaatgt tcaatacttt ggatagtgaa tgtgaaggca
     4861 acttcattga aaaagaaatc aatatctttc agataggctt gaacatcttt gttaattggc
     4921 gtttcccgat tttcatctaa atcgtcctct atttgtactc tttggttttt aaacatctta
     4981 tcccaactct gatttattat gatgtctttt tcttttacgg gaacgtcagc atctcctaaa
     5041 gtattaaagg taaattgcag aaacgaaatc atccgtgcaa catcttcctt atatcgttca
     5101 agggcttctg ccgaaaggga ttgctcgcta atctccctct gtgccattgt tggtaaaatg
     5161 gaccaaagaa aaatcaaaag aaaaagtgag ggtttgaatt ttgtgatcat ttttcaaatt
     5221 aactcttatt gatctgatta aaaaattttg aaaaaattac tcgcgatgat ggataaaatc
     5281 tatgattcta tctttttgtc catcctccag ccttatcaaa ttggaaataa gccatccttt
     5341 gtttaaccca gggcgattaa actcactgat ttccatgtac caatttttca attggaaaaa
     5401 atgaaatttg acgttagtaa cttgtttaaa acgcaactta ttggttctga tttcataaag
     5461 aaatattgag agtttatcaa cttggaaccc ttgaaagaaa taatctccaa tgtactcttg
     5521 ggaatcgaat accttatcca gattcatgaa atccaactcg tggcttaagg ggtgtaaaaa
     5581 acgtgaagcc gatgtcccat taggtttata aagattttcg taaggcgggg aataaacatc
     5641 atttattatc catttcgacc caaggttctc tttaacaagt ttcataaaca gggtgaatat
     5701 taccatttcg tttcctctct gaaaaagcac ttctatttca ctaaaccagt cgcccccatg
     5761 aaaatccaga aattttggat ctctgtcaac aaccatatca tttacaaaag catttttcaa
     5821 caactcagga atatcgctat ttgaatggtc gaataacatg ttgaggtatt ttttgcgtaa
     5881 atcggatgaa tgatatgtag gatcgtttgt tgctaactta actcccctta catcttcctc
     5941 tccgttaaag cgtcggataa attgatttac ctgcttggtt tcagcataaa atgaaacttc
     6001 atcggcggca gaaataccac ttcttgataa aagaacacca ggctcctggc tatatacctg
     6061 tattaccaca aaagccaata gaaaaaaaac gacttttact tttcgcattt ttgaattgat
     6121 ttaatgatta aagtgatagt aaacaggttg ttatattcac aatttaattt taaaactatg
     6181 ttgattaata aaatcgccgc aaaacgaagt tgatttatca gaaacgaatt gcggcgataa
     6241 ataattattc gagttttttt aaggcatagt ttctgagact ctgatgtcgc ctaaaagaac
     6301 atcccagaag cctatcaggc gtccgcgaat ctgggtttct ttacgctcga catagacagt
     6361 aatgtctttt ttcgtggtat ctacatatac cagattgtcg cctactcttc cttcaaatcg
     6421 ctgataaatg gttatcacgc ctacatagcg gccatcgggt tgcttttcaa ggtcgctgat
     6481 atattcaata ttgtaccact caatagttac tttatcataa ttaagagcca taagtctttc
     6541 aagataggta cgtatccccc agtatttaat ctctttactg ttaagggtag atacccccat
     6601 cattgcgcca tcggcaaaaa gttcgagcgc tctatcaata accctgtttg cctccgacca
     6661 cggtgttttt ttatcgccaa taatgctgat atattttcct aaatccctta ctttttccaa
     6721 agcaaggctg tcaatagcat tttttcgttg aggactgacg ttatcctggg caaaagccgg
     6781 aatactgata accaaaaaaa atagtatgat taatgcttta tttttcatct ctttttactt
     6841 tttaatgagt tccaattcaa caatttttcc tttagtgtcg tactcaatat tatgaactct
     6901 gtttatattt tttttctgat ctttaagatg ttctaaatat tgttttatag tagttggacg
     6961 atcataatct gttatttcat tatcctgata aacaataatt aatacgggga cctctggcga
     7021 tgcaaagaat tttaaagcat cgttgatccg catattagca atatcaatag tagaggcact
     7081 ggcgatagca tcaaaataat cctccatttt atcaaatttt tgttcctcta tctttctttg
     7141 ttctgctgct tctcgttctt tgcgtaatct ctcttcttca agtcgttgtg cttcaagttt
     7201 tttatgacgc tcaatagctt tttctgcttc gataattaaa gcatctacct cttcattatt
     7261 aaggtttaat gctttaacat catccacaat tttttctttt tgcgaaatgc tcattgcgcc
     7321 ttgatcattg attaccatta aaaggtcttt tttggctttt tcaattttct cagcaagctc
     7381 ttttgcggct tgctgtctgg caagcttctt tttactttga caaccggtaa aaccaacggc
     7441 tatcatagct gcaatcaaca aaaaaaccag ccggatacta attaggtttt taatttttaa
     7501 aagcataaaa gtaaataatt aatgtgttga tttttaattt attaaaagtt aataaaagtt
     7561 taaaaagata taatcattgt taaatctgag taagtattta aagcttacat gtatgatgga
     7621 tgccctgtaa acggtaattc agcttaaaat atttcaaaca acaagcataa ttagtcaaaa
     7681 ataaaaaaag ctttgaaaaa aaacattgac acaattgatt ttgaaatacc agatcttacc
     7741 ataattaaca gaaggtatgc aattggttta acttcttaca gttttaacag gtctgcaatt
     7801 tttgatggaa tgctgttttt attcaccatt acataaattg tcttgtaacg aaggtatgat
     7861 tctgatgcat aaaagaaccc atcatagata tggccatcgg ttccccatga atttttgata
     7921 atgtaaaaag tgttcccgtt ctgatctttt gcccgaccaa ttatttccat aagatggtca
     7981 tctgttgtgg tgtaattatc aaatcctaat tgtctggttt gttgagttat caatttttct
     8041 ttaatcggtt cattgaacga aaacaacatc ttatcacgct cttctttagt tagcgcttcc
     8101 catcgttctt tttctgttcc tgttaagtca ggctttgttt catcaggaat aatagctaat
     8161 ccgtttttcc acgaaaaccc cctttcgcta acatctgccg accaggcaag ggtatggcct
     8221 ttatcaagcg aagtattgat tatttccaaa agctcatcaa gttttacgtt gtaaatctcg
     8281 cctaataacc aattgtcagg aatttcgatg ataaatttct gatagtaggg atgatgagta
     8341 tatgaaccga cctcaataaa atcatcgagt tttaatccta aagcatcagc aaaagagcgt
     8401 ggagaatact ctttgccttt atatgtaaat ttttccggat agctgcccag atatgtatca
     8461 agtaagcttt caaatccttt ttgccagaca ggagtaagct tacggttttt attctgtata
     8521 acagcgtcaa cataattttt aaaaatcaaa tccatttcgg aatggacaaa catttcgtca
     8581 tcagttacaa ggcctgtata aacctcttca ggaactattc catatttgct gataatatga
     8641 agcacatcat gagaggcgcc acctgccgcg aaattttttt caccatgcca acgaacatat
     8701 tgaatggctt tttcaagata cgactgacgg acaataaatg cttcggaaag gttgaattcg
     8761 ccctttccac ttttcaataa ctccgactca ataaacgata tgccagcata gctccagcat
     8821 gtcccggaac gatattgatt ttttacagga gtacaattta accggatttc atcagtgaaa
     8881 acatagccct ttttttcagt ttctatttgg gcatctatgg actgaaaaaa gagtgtaaaa
     8941 cacaaaagaa gtgttacata gaaagaatta attttcattt tgctattaat ttgaagtgaa
     9001 aaacaaatgc aaaactaata aaacagagct attcataaaa aaaaggccgc taaacaagtt
     9061 agcagcctct ttttcttatc gggaaaacag atattaccaa ttttggtatt aaaatttcat
     9121 cattctgttc gaatgattta attttaaaca aaaccaccga aaaacaacat atgaaataat
     9181 tactcgcaag ttaaaaaaag tcaatgtctt ttattcggca gtaaatttcc cgatgcattt
     9241 taacctaaaa accctgttca ttatgaaaac caattactct cttaaaactc ggaaaacgtt
     9301 acatttttaa ataaaaaaat gaactgttat gattatgaaa tgtgaaatac aaaatcaatg
     9361 atgcaaagat agagttgctt tccaaaatga atcacagtta aaaaacgtat ttttcaattt
     9421 aggtattttt acctatgcat ccttgatttg ttaatatcat taattaatct cgctggagat
     9481 ttaagttgtt cctttcttaa tttcatgaaa agtggttatt aattctgctg aagttgtatt
     9541 taccaataga taggccgaag cgcccgcata aattatttca tttattaaag ttttgtcagt
     9601 aaaaaatgtt ggtaattgca ataaaatcaa agagtttgtt atataaaaag cttattcatt
     9661 tttcaagaga ttatttttta tggcaaattt tactaaaccg gctgtatttc ttgatcctgt
     9721 tttttcaaga agattgcgcc tatgtgcatc tacagtgcgg atgctaataa agagcttttc
     9781 tgctatttct tgatttgtat gctcatcaat aatataccga agtacatcta attctcttgg
     9841 tgtcaaaggg ataatttcgc cggatatctt tccttgatta gttttttttc taattaattc
     9901 ctttataatg acgtttttca cttcatcgct gaaatagttt tcgccattca ttaatgtgtt
     9961 gatagctgtg attagctctt tgcggcctga attttttaaa atgtatccac atacgccgct
    10021 cgccaacatg ttttttatat gctcatgctc gcttagcata gtgagcacta aaattttcac
    10081 tttggggaat tttttgccga tttgttggga acactcaata ccattcagta ttggcatgtt
    10141 aatatcgagt agaacaaggt cgggtgaaag tttaccgatt ttgtcaatag cttctaaccc
    10201 attttcggct tgtccgacaa tttgaatgtt ttcctctcct tgaagtaatg aaataatacc
    10261 gtcacgaaca attttatgat cgtcaactaa aagtacttta atggctttta tgtttttatt
    10321 cataatttac aatggtattt gaattgttat tgctgttcct tttttaggtg tactgtcaat
    10381 ctctaaacta cctgataatg ctttcactcg attactaata ctaactaatc ccatcccttt
    10441 aaactctttt gtcgggcttt taatattaaa tcccttccca tcatcttcaa aggtgtatat
    10501 tatctcattc ttgtgaagta acagttgaat aaaaatagta tttgccttag catgttttaa
    10561 aacattgttc agtacttcct gtgtgattcg gtataggttt agttcaatat ttctgggtag
    10621 gcgatagtca gttttgaaat tttcaaaaaa ggtaatttta atatctcctg acatttccac
    10681 tttgttgaaa agcgatttta gtgaaagaat taagccgtaa tcctctatag cttttggcat
    10741 aaggttcaag gctatattac ggctctcttc aatagccatg tttaaaaacg aaaaacccaa
    10801 attatatttt tctttttctt tatcattaag gttttcaaca agatgtttta cagcattgaa
    10861 gttaaggctt gccgccgtca ggttttgccc aaggctatca tgtatttctt tggccactct
    10921 tgttcgttcg cgatcctcgc cttcaataag tgagctaata accatttttt cggtttctcg
    10981 taagagtgta atatcgttta tggcggcaat aacataattc ttatcctgaa catctattat
    11041 ttctgtggta acttcagtta tcattttcat ttttgttttt tttattaaag tcatttctgt
    11101 gatcttggat agctcagctt ttactttttg tagcaactct ttattagtat aatcagcgat
    11161 ttctggtctt agttccttga tgtcggtatt tagtagttcg tgatgtgtat aaccaaaaag
    11221 cttctcggca gagttgttgg catcaatcag tttaaagtca tcaaggctga aaattataag
    11281 cggaacggga tttgttttaa ataaaagccg ataacgaagt tcagattttt caagttcttt
    11341 ttgtgcaagg atttggtcag tattgttaaa ctggcttccc caaattctga caagttcacc
    11401 attaacaata atgcctacaa tgttattaga tatgtaaagt gggtttcccg acctgtcttt
    11461 atcaattgaa atctcgttgc ttacccggta attggaattg atgagcttct taagaaaatt
    11521 tttgttgtaa aaaaaattat ctccaccttg caaccggcta agggtaaatc cttccatttc
    11581 ggacgagtgt tcgtagccat acatatttgc gaaagaatcg ttgcaagttt tgatatatcc
    11641 atggttaaaa ataatatcta tttgttcttc aacaggcata gttggaacta ctggtttttc
    11701 aagctcatat aaaaaaatac cttcgtttgc gtattttatg aagctgcgat gaacttctaa
    11761 ccaataattg agatcaagct cgagttgttt tttggttgta atattctgta tggtgccaat
    11821 aattctgcag ggctggttat tttcatttcg cttagcaact attccaatta aatgcaacca
    11881 tatccaatcc ttattttgat acaaaaagcg atgttctaca tcaaatgttt caatattgct
    11941 ctggagcatt tcttcgaaag aggccattac tttagacgta tcatcagggt aaatattatt
    12001 tgtatatttt ataaagtcta ttattttttc gggttcaaca attcctgtga tcttttccca
    12061 agtgttgttc actaaaattt tcctggtttg aagatcgtaa tcccacaaac ccaaattatc
    12121 gcctaacaaa gctatttcaa gtcgttgatt tgtttctatt agtttttttt tccgataatt
    12181 ttatgctggt aatgtctttt gaagacccta ttattttact gacgttgcca tgttcatcca
    12241 ataaagtgtg aacattatcc tgaagataaa tggttttacc gactggatta gtaaattgtg
    12301 tttcaagctt tattgaagta attccttgct tcagataatc gaaataaagc tttttattct
    12361 tttcatttga atcttgattt gtaaaagagt ttattttttt tccaatcatc tcttcgggat
    12421 aaaatccgta aactctctta cttgcttgat ttatgaaagt gataacacca tttttgtcca
    12481 aagtccaaat tacatcatgt gtaagaccaa caatctgttt ataaagataa tcactctcat
    12541 taagctgatt tttcagctta accaccattt ttatctgtgt cctgacgatg aaataaatta
    12601 ccaacgctgt aataacaata aaaaaccatc ctttgaaagt ttgcatttgt tcccttacat
    12661 tgtaatcact aacgagatag ttcaatactt tatcggaaag aagaatccaa aaaaatccga
    12721 aaattagaaa gtaaaaggct actttaactg cagggcgatt gtattcattc aattttcgca
    12781 taacagggtt tttactgggg agctactctg aaaaaaacca gagcaattac tttttgatga
    12841 gcatattttt tatatggtta agcttcataa gggcctctac cggagtaagt gtttcaatgt
    12901 ttatgttgat aatttcatct tttatttgca gcagtaaagg atcgtcgagc tgtatgaaac
    12961 taagttgata atcattgtca gtatgtggca agtttaactt tttgttaagt tcatcttgac
    13021 tgtggctttt ttcaagaact ttcaacattt tttctgcatg ttcaatcacc tcagtcggca
    13081 tgccggcaat ttttgcaaca tgaataccaa aactatgttc ggtgccacca ggagaaagct
    13141 tacgtaaaaa aataacctta ttgttaattt ctttaaccga aacatgatag tttttaatac
    13201 gtttcatggt ggttgccatc tcgttaagct catgataatg tgttgcaaac attacttttg
    13261 gccgaaaggc aggatgatta tgaaggtagg ctgtaatagc ccatgctatt gaaataccgt
    13321 cataagtgga agtgcctctc cctatttcat caagaaggat aagactacga ttggatatat
    13381 tatttaaaat acttgcagtt tcgttcatct ccaccataaa agttgactcg cctgaagata
    13441 tattgtcaga agcgcctact ctggtaaaaa ctttatccac caagccaata gatgccgatg
    13501 atgccggtac aaaacagccc atctgcgaca taagaactat taatgctgtt tgccttagca
    13561 gtgcagactt accggacata ttaggaccgg tgataataac gatctgctgt tttttattat
    13621 ccaaatagac attgttagga atatatgctt cgccaggcgg catttggttt tcgataacag
    13681 gatgccgacc gttttttata tctataatga aagaatcatt gatttgtggt tttatatagt
    13741 tattcttgat tgctaccgtt gcaaaggaga gaagacaatc gagcctggca attaacaatg
    13801 agttgagctg aataggttcc gtgtagtctg ctaaggataa aacaaggttg ttgaagagct
    13861 gtgtttcaat ttcaagtatt ttctcttcgg cgccgagtat tttctcttca tactctttca
    13921 actcctgagt gatatagcgt tcggaattaa caagcgtttg tcttctttcc cactcttgag
    13981 gtactttgtc tttatgagca ttggttactt caaggtaata gccaaagaca ttgttaaagc
    14041 caatttttaa agagactatt cctgttcgtt cggcttctgt tttcctgatt ttttccagat
    14101 attccttgcc cgaataaagc aaatcacgta attcgtccag ctcctctgat actccctggc
    14161 gaaacacatg tcctttatta atagatactg gcggatcagc ttccaactcg gcttcggttt
    14221 tgtttcggat aacatgacac gggttgagct ggtcagctat ctttttcaat ggatcattat
    14281 tagttaccgc gcatccatct tttatttttt caatagcgtg caacccccgt acaagttgta
    14341 ttacttctct tgggtttacc cttccaacag ccacttttga aattaaacgt tcaatatcgc
    14401 caatcagctt aatgttttct ctaaaaatct cggatagctc tggattgtca atgagatatt
    14461 ctacaacatt cagtcgttct tcaatactca tttttatttt tagcggcatg gtaatccatt
    14521 ttctcatcat ccttgatccc atgggtgaga ttgttttatc aatcacctga ataagtgtag
    14581 ctgcattttc gttaggcgaa tggagaagtt caaggttgcg gatagtaaat ttatctaacc
    14641 atacatattt gtcttcttca attcttgata tttttcggat attggccgac tggtgatgtt
    14701 gcgtttcggc aaggtagtgc aaagatgctc cggcagcaat tatccccata ttaaaatcct
    14761 caaccccaaa accttttaaa gtatttgttt ggaaatgctt cagaagcagg tctctggcat
    14821 aatcttcagt aaatacccag tcatcaaaac ttgtcaaata atagttttcc ccaaactctt
    14881 cgataaattg atttcgttta tttttttgaa taattatttc gctgggttta aagctttgta
    14941 tcaatttatc caagtattca tttgtccctt gggtaaggta aaactcaccg gttgatatat
    15001 cgagaaatga aatacctgat tctttaaatg aaagatggat gctggccagg aagttatttt
    15061 ctttgtggtt taatacctta tcgttgaacg aaacgcctgg agtaacaagc tcggtaacgc
    15121 cacgttttac aattttttta gctaatttcg gatcttccaa ttgatcgcaa atagcaactc
    15181 ggagtccggc tcttactaat ttcgggagat aggtatctat tgaatgatgt gggaatccgg
    15241 ctaattcgac aaacgatgct gctccattgg cgcgtctggt aagaacaata cccaaaatgt
    15301 ttgaagcttt aatagcatca tcgccaaatg tttcataaaa atcaccaacc ctgaacaaca
    15361 gcaatgcatc aggatatttg gccttaatgg ccgaatattg tttcatcaat ggggtctcca
    15421 ttgccccttt taaactcttt ttggtcaagt gattaatttt ttaacaaaaa taacttaata
    15481 atcctattat tttagacgat aaaaaagcaa atttacaaat aaagttgtgg aaaatgtacc
    15541 taagaatcta aaatcgtata ttacaaagaa ttcgacgggg cagaagtatg tattactaaa
    15601 aatgtaatat gaattaattt ggtcaagcag aaacaagaat taaccgatct aaattttttt
    15661 tgtgaatgat acttttaagt tgatttttgg aaatagaaag ttttgttctt gttgttaata
    15721 aaattgtatt tttgcaatga cttattgaaa agtgtccggg gtgtggcgca gttggttagc
    15781 gtgctagact gggggtctag aggtcgcgag ttcgagtctc gccactccga caacaaaaga
    15841 ggctgaataa gaagaccagc ctcttttgtt ttataatgca gttgggttaa tgcaggaaaa
    15901 ggctcatatc atccagcact tttaccatgt tacgaaaatg ctcagctgag tcgtgcataa
    15961 acagggaaaa tgcgtttttg attatcaaca atgcatgtat ctctttaggt gaaatgtcga
    16021 gcgcttcagc taaaaaagcg gtatagcggt cggtatctaa aatacctgcc ataccaagga
    16081 ctttatgact gggcttattg gatgtttttc atactatata tgtaattacg tcaaggggat
    16141 tggaaacgat aatgattatg gtattggatc gaaccaaatt atcacggttc attccgggct
    16201 tttgtagaac tcctgaagtg atcaccacta tacccgagcc tttagtgtgt aaatagttat
    16261 tggtaactcc agcgacacgg gtattgaagt tgttgataac agaagtctgc caaatatcaa
    16321 gagctttacc ttcggctaat ccctctttaa tatcaactaa aacaacttca cgagcaagat
    16381 ctttatggac gatcacattt gcacaagttg cgccaacatt gcctgcgcca actaaagtga
    16441 ttctttcctt aaattaaaat ttaaaacaat ttttcaaaat gactaataag caaatttaag
    16501 tacaaaaata ccattattaa ccttgtctat tttgaaaaaa acagttataa attttggaat
    16561 attaatggtt aaatccaata aaaaaattgg atttagagca atgcgttggt ttttgaatta
    16621 agcgatttag cagcaagtag aaaagcgata agaataactc ctccaccgat taagctaagc
    16681 aaaaggtaat ctcctgataa taaacaaaag ctataaaaac cgaataaaat cacggcagca
    16741 gaaaaacctg tcagggaatc aaaattatta ctcctgaact tggataaacc aataaaaaaa
    16801 cccatgataa tggaaataaa aaatactgct acaggcaaag tataaagtac gattctaatg
    16861 ccatcagtat atctccattc gtaaaaaaaa tagacattgg cagcagttgc atatcccatt
    16921 gcaatcatta ccgaaaataa aataccatca aaaggtttgg taaaggactt tttgggtaga
    16981 taataaaaac gaagtaataa aaacttgaat aattccgaca aaaatccaat cagtacaaaa
    17041 gagtaaaaga caatgcgcct aaggctctga acatgtgtaa gccaatatga ataaacaaaa
    17101 tatagaacta aaatcattgg tatggcagta agtaacccaa gaaaataggc gcttgcaaaa
    17161 agattcagaa cttttttttc cataatcttt cttgcaaaaa aaataagaat caggaaaata
    17221 aggggagcaa taagcaatgg caagtacttt aaaaaattca taattttgga ggctttatta
    17281 tgttacatat aaacttttcg caaatataaa tctttaaata acttaactgt acagaaatga
    17341 aaaatatcac aataaaaaag ctgacagatg aagaaataga taaaaatgcc ataaagagct
    17401 ggcctatttg ggaaaaagaa gtatcaaagt ttccatggtt ttatgaagct actgaagatt
    17461 gtttaatcat tgaaggaata attgaggtag aggccaatgg gaaaatatat caattaaagc
    17521 ctggagattt tgtaactttt caaaaagggc tgaaatgcac atgggatata aaaaagcctg
    17581 taaggaaata ctataacttc agatgattca tggataattt attattaaat ctttaattga
    17641 gaggggtgaa aggagtcgtt ataaaatcta cgggtagctg gtttactgtt ttggatgaaa
    17701 atggccggca aattaattgc aagttaaagg gtagattcag aataaaaggt attaaaacca
    17761 ccaatcccat agctgttggc gacaaggttg agtttgacat tgccgaggat gaaaagattg
    17821 gtcttattac aaagatttta ccgcggcaca atcatattat tcgtaaatca acaaaactct
    17881 ctaaagtatc gcatatcatt gccgccaata ttgatcttgc gttgctgatt gtaactatca
    17941 aagagccgga aatcagtccg gggcttatag atcgtttttt agtaactgca gaagcttatc
    18001 atataacttc ggccatcgtg tttaacaaaa ttgatattta tggcgatgtt gattttgaca
    18061 aacttcagaa atatgaagct atttatagag ctgccggtta tcaaactttc agagtgtcgg
    18121 cattaaccgg agaaaatatt gacatgctaa aagaggtaat acgagataaa atatgtctgt
    18181 tttctggagc ctctggaaca ggaaaatcgg cgcttatcaa tgtaattgag cccggactaa
    18241 aactgaaagt cggcgatatt tcaacatacc atctcaaagg tcggcacact actacctttc
    18301 cggaaatgca taaactcaca ggcggagggt ttattattga cactcccggt attagagagt
    18361 ttggccttgt agagttccgt aaagaggaga ttgccgagcg ctttcctgag atgcgggcat
    18421 ttatgcacga ttgtcagttt aataattgta cacacataca cgagccgcga tgcgccgtaa
    18481 aagcagctgt tgaaaatggg aaaatagccg cttcacgata tgaaagttac ttacgaattt
    18541 ataatgatga ttatcttgat aaagaactat gggaatggga ataactttta tcgtttacct
    18601 catttaacag taaaatattt aaatttagac tttttttaaa cgttgtgtaa tttaccaatt
    18661 ctaagatttt taaaatgata attcgtaacc tcacttatga ttttttccaa ccgctaaaaa
    18721 aatctgataa agtatcgtca gcgctccgat taatgtacga atataaaata tcacatgcac
    18781 ctgttgttga agagagcacc tatcttggaa ttatttcaga aaacgactta ttggtaatgc
    18841 caaacaaatc agaaaaaatt gaatcacttt gtaaagcttt acctaagcca tttattaaag
    18901 aaaacgaaca tatactcaat gcgctaaata tcattagtaa cctgcagatt gaagtattac
    18961 ccgtgttaga caatgatcat caatataaag gtctgattac tgctgagcag gtattgaaag
    19021 agattgccgg aatattttca cttgaaaatc ctggtggaat tattatcctt gaagtaaatc
    19081 taaacgatta ctccatgagc gaaatagcaa gaattattga agctaacgac accaaaattc
    19141 ttagctcagg agtgaaaata atacctgatt caacaaaaat tgaaattacc ctaaagttga
    19201 atgtaatgaa tattgaaccg gtaattcaaa cttttatgcg atacaattac aacatcattt
    19261 tttattttgg agataatgaa aaaaatgaag aggtgttgaa agagaggtat gaactattaa
    19321 tgagatattt gaacacataa agaacttatt cgcctcacaa aaacagctta tcggatgaaa
    19381 tatcttttac tgtttttaat aataatattt ccttttgccc aaagtgcaac accgaagcag
    19441 cttagctcga tcaagtcgga aataccaatt gatagtatta ttgaggcgga gaataaaact
    19501 atctctgaat catcattcgt cattgattca ataatattaa cgggcaataa aaaaaccaaa
    19561 gatttcctga tttttcgtga attaactttc aaagttggcg atacgcttaa tcataaccaa
    19621 cttcaggaaa actatcagaa atcaagaaat aacctgatca agctttcact ttttaatttt
    19681 gttgacttta ctgattcact tgtagctgct gggcaatatg ctcatcttct tgttcaaatt
    19741 tcatttatag aaagatggta tttgtggcca tttcctattt tcgaaatttc tgatcgtaat
    19801 ctgaatgttt ggctaaaaga gaaaaacttt aatcgtatca gttacggtat ttatcttatc
    19861 aagcataata accgtgggcg aggagagctt cttaggcttt accttcgtag tgggtatgat
    19921 gagcgatatg aattaagtta tcaggttcct tattttaatc acaagcaaac gcttggagct
    19981 acgcttggag cgggatggct gcaaaaacat gaagtcccgt atagaaccat tgcaaacaaa
    20041 caggagttta tcaaagatga tgatcattac cttttcaagc agttttataa ttttataaat
    20101 tttacatatc gcccagatat tcatcagttt cacaactttc agattagata taactttttc
    20161 tatttcgatg acactttact taagctaaat cctaaatact ccttcaaggg attaaaaacc
    20221 aatgaatata tgacctttgc ctacaagttt attagcgatc atcgcgattc aaagatcaat
    20281 ccgctgacgg gccattattt tgaaggacag atatctaaaa gcggctttgg cttactgaag
    20341 gatggataca ttgaaatgat ggatttaaca ggatcgtaca ggaagtattg ggaatttcca
    20401 aaacgctttt acttctccac cgattggacg ggaaagctct ctaccaatcg tgaacaaccc
    20461 tatttctacc aacgcgggtt tggttacgaa cgcaattttg tgcgcggata cgaactttat
    20521 gtaattgacg gtcaaagttt tattttagga aaaaacactt taagatacgc ccttgttccc
    20581 gaacgaaaaa caaccatcgg ttttttaaaa tccgagaaat tcagtaaaat ccattacgct
    20641 ctttttacaa atcttttttt agatgccgga tatgttgatt catttcgatt ttacgatccc
    20701 aatgatcttt ccaaccatct tatttatggc gtaggtttag gaattgattt ggtaacctat
    20761 tatgatatga ttttcagaat ggaactatcg gttaataaac aaggtgaacc cggtttttat
    20821 atccatttta aagatacatt atgattaagc aagatgtatt ttaaaaacct aaataacaaa
    20881 agtctttgta gatacaccta acaagcaatc tgcaagccct atgcttcaac aaatatttta
    20941 tatggttaaa gaaaaccaaa tacctttgct aaaagttaaa taaaggataa tgctgaaaaa
    21001 gatagccatt tttggaaaat caatagaaaa ggagggtatt gaatatttac aaaacctatt
    21061 aaataggttg gagtttttca attgcactct ttctatttat gaaccgtttt ataaaaaaat
    21121 tgaaaatcaa gtacggttcc catcaaattt aaaatttttc aacacacacg aggagcttaa
    21181 agataatgca gaaattcttt tatctattgg cggggatggt acgttgctcg acaccattac
    21241 attgattcgt aactcaggaa tcaatgttct tggtattaat cttggccgat taggtttttt
    21301 atcaagtatt tcaaaagatg aaattcttcc ggccattgac aaagttctca gcggaaattt
    21361 tgttctcgaa caacgaacat tacttgagct atcgaaacct gaagcaattt ttggggattt
    21421 taattttgcc ttgaacgaac tttcgattac aaaatcaagc cctcatgcac ttgccgttat
    21481 taatgtattt gtaaatgata gttttttgaa caaatattgg gctgatggtc tattggtagc
    21541 tactcctaca ggctcaacgg cctattcgtt aagctgttcg gggccgataa tcactccgga
    21601 atcagaaaac tttgtcatta cgcccatagc cactcacaac ctcactgtta gacctatcgt
    21661 tatacccgac aagtgtacta ttcgaattac cgttgaagga cgaaatcaaa attatcaggt
    21721 cgggctcgat tcacgttttc aatcattgag aatgacagat gaaataataa ttaaaaaagc
    21781 caattataaa atcaacctga tacaactaca cgaaaaagat ttcttcaata caatacgcga
    21841 aaaactcctt tggggaaaag atcaacgcaa ttaaacctca agtgattgat aaaattgtcg
    21901 gccaagataa agcttacata ctaaatcaaa ataagttgag tttaaaaagc tgagtatcgt
    21961 tgatttattc atttatgaag catttcctaa agcttttcat atacatggcg gctctctctt
    22021 tggtgttttt atcctgtaac aaaatatatg aggacaaaat accgccaaca attattttgc
    22081 aaggacctaa tccctacagg ctttttacaa gttgtagtta ttcagagccg ggatatatta
    22141 ttaaagatga ccttacacca aactctcaaa ttaaagttga tatttatgat tcaactttaa
    22201 acactaccat cccgggagat tattatattg attattattc aaccgattta gatggtaata
    22261 catcttacgt gaggcgtaag attgaaaccc gaaagatgac tttagattat ttcaactccg
    22321 gttttatagc ttatgatacc ctaaagccga tgaatattaa aactcaatat tcaataaaaa
    22381 gcgtgcttta ttcacaggaa ttcaaatgga tacgaattga aaacttcaat aatttcggca
    22441 agcaatttaa ggtgatcatg attcccgaca cacttggcaa ccttacgtta aattacaatt
    22501 ttaacgatac aattgttttc ggtacgggta atgtttattg caacatgaca ggattcaggc
    22561 ttgagtatca gattgatgtg cccgaaaatg acatcgaaat gcattatgtt acctacaggt
    22621 ttttaaatta agaatattac cagattttat gaaaatcatt aaacattgtt taatcgtttt
    22681 actggccttt ttagtaaaaa taatttatcc tcaagatatt gacataggta tatttggagg
    22741 tggttcatat tatttgggag agcttaatcc gggcaggcaa tttttaatga ccaatcccgc
    22801 ctatggtggg ctgatccgtt ttaatttgaa tgacagaatg gcgttcagag gagagttaat
    22861 tcatggagtt gttagcggcg acgatgcagt gagtaaagaa aatgaactca gaaatctacg
    22921 tttcacatca actataaatg aattatcggt actttttgaa ttcaattacc tcgaatattt
    22981 taccggaagt caaatccatt atttctctcc atttctttat tgtggacccg ccttgttcac
    23041 ttttaatcca aaggctaaat tccgcaatga atggatcttg cttcgggata ttggaaccga
    23101 agggcaaatg attaacgaaa atgataatcg ctacaacctg ataagttttg catttgcttt
    23161 tggtattggt tttaggtata gcttttctca ccgcttagga tttactgcag aatgggggat
    23221 gaggaaaaca tttaccgatt acttggacga cataagtaaa aattattacg ttgactttaa
    23281 taatttaaac cctgacgata ttagtcttga gaagatgctt tccgacccat ctcctataaa
    23341 acaccaacct ggaatgcagc gtggcaaccc acaaaacaac gattggtatt catttgtagg
    23401 aattacgctg acttatcgct tcacaattgg tgaaaaaact acctgcgttg aatttcagaa
    23461 tagatgaaat agtaatactt ttgcaacctt attgtcataa tattttctag tcataaaatt
    23521 ttaaattcaa taggatcaaa ggattataaa tgggatttaa agataaaata gatatgaaac
    23581 ggctcccaag gcatattgcc atcattatgg atggcaatgg tcgctgggct aaacaacgtg
    23641 gcttagtccg caccgttggc catgaaaatg gtatagtagc cgtgagagaa gttgctgaag
    23701 ctgccgctga acttggcatt gaatatctta ccctttatgc tttttctgtc gaaaactgga
    23761 aaaggccaca acgcgaagtg gaaattttga tgaagctgct gctaaaaact attgattcgg
    23821 aaatgaagac cttgaaagaa aatgacataa gattactggc aatcggaaat ttaatatcgt
    23881 tgcaaaagaa aacctatgaa cgtttattgt gggcaataga agagacaaaa aaccacaaac
    23941 gcatgaatct tgttcttgcg ttaagctata gctcgcatta cgagattgtg gaagctgtaa
    24001 aagaaattgc cagaaaagca aaacaaaatg aattatcccc tgaagacatc aatgagtcag
    24061 tgatttccaa tcatcttttt actaaaggtt ttcccgatcc cgaactgtta attcgcacaa
    24121 gcggcgaatt caggatcagt aatttcttac tttggcaatt agcgtattcc gaaatataca
    24181 taactcctat cttatggccg gactttagaa aggaaaactt ttatgaagct attgtcaatt
    24241 ttcagaaaag agaaagacgt tttggaaaaa caagtgaaca ggtaagtgag aatgcaaaca
    24301 aaatgaaatg aggaaaatag ccagcctgat tcgtaataac tatatcttac cggcaaacta
    24361 tttgttgttg ctggtatttc ttctcaacgt tccccatttt agctttactc aaattaatat
    24421 cggtccccca gaactggata ttgattacat gaacccagtg gagtatgaga ttggcggtat
    24481 aacggttacg ggaatcgaat ttttggatcg taacgtgcta attatgatct caggtctaaa
    24541 cgttggtcaa aaggtaaaaa tcccaagcga caaattttct gacgccatag aaaacctttg
    24601 gaaacaaggc ctttttgaac atgtaagcat tacagctacc gatgttcaag ataaaatggt
    24661 ttttttaaat attgatttac gcgaaaagcc aaggctgtca aaatacacat tcgaaggggt
    24721 gaaaaaatcg gaaggcgata accttaaaga tgaaatcaaa atcagttcgg gcgatgtagt
    24781 aactgaaaac cttattacca attctcgcaa caggatcgta aattattatg caaaaaaagg
    24841 atttttcgat gccgaagtgg atatcagaca aatttccgat acaacacggc tgaacagtgt
    24901 gattttaatt tttgatatta agaagaataa tagaataagg atttatgaca tcgaaattac
    24961 cggtaacaaa aaattgagcg atcagcaagt attacgcgct ttaaaaaaca caaaggaaaa
    25021 aggtgcgttt cgacccttcg acgaaatcga aacattgatc tgggatgtaa ccaggcacgc
    25081 tctcttgctt cagtttgaca ctctatatgc caaagccggt gaatatctta ataataacat
    25141 gaaattaagg atattcaaaa cgtcaaaata tattgaaagc gaatacgaaa atgataaaca
    25201 gaatcttatt gagaaatata actctttagg gtatcgggat gcaaaaatcg tccgtgactc
    25261 tatttaccgt aacgacgata tgacgatgaa tatcctgatt gaagtggatg aaggccctca
    25321 atactttttt ggcaatataa gctgggtggg gaacaccata tacaccgatg aatttttatc
    25381 tgatgtgctt aaaatccaaa gaggagatgt ttacaataaa gaaatgctta ccactaatct
    25441 ttctttcaat ccttcaggtg ttgatgttag ttcattgtat cttgatgacg gctatctctt
    25501 ttttcaggct atccctgtcg agataagggt tgagaatgat accattgatt tagaaatcag
    25561 aattcgggaa ggtaaacagg caaggataag taaagtaaac gtaaaaggga atactaagac
    25621 caacgatcag gttgttgtaa gggagttgcg ttcccgtcca gggcaactat ttaaccgttc
    25681 caacataatc aggacgactc gcgaattagc acaattacga tattttgatg cagaaaccat
    25741 caaaccggat atccaaccta accctgctga cggcaccgta gatataatct atgaggttga
    25801 ggagacatct gccgatcaga ttgagctttc gggaggatgg ggatatggta ggatattggg
    25861 caccttaggg ctttcattca ataatttttc gttacaaaac ctcttcaaaa aagaggcatg
    25921 gcgaccaatt ccatcaggag acggacaaaa actttctatc agactacaat cgtatggcag
    25981 aggatacata agttacaatt tttcgttcac agaaccttgg tttggcggta aaaaaccaaa
    26041 ttcattcagc gtttcttatt ttcactccct ttactccaat ggtctatcaa aaaaagaccc
    26101 ttcacgatca gcgtttctta ctgacggggt agtattaggt ttaggtaaaa ggctacaatg
    26161 gcccgacgac tactttacac tttatcaagc tgcaagtttt cagatttata aacttgataa
    26221 ttatgcaagg attttttcgt ttggcacagg taacggtaat tacaacaatt ttaactacaa
    26281 cttcattttt gcccgaaact ccattgatgc ccctatttat ccgagaagtg gctcagaggt
    26341 atcgttgtca gttgctgcaa caccgccata ttcggcattt aacaacaagg attataaaaa
    26401 tatgcccgta gaggaaaaat acaaatggat tgaataccat aaatggaaga tcaggacaaa
    26461 tttttacatg aatataattc aaaaccttgt attaagcacc agggtacaat ttggcttttt
    26521 gggtacctat aatcacgata taggagttac accctttgaa cgattttttc ttggtggcga
    26581 tggattgtcg gggtacaaca acctggatgg ccgtgagctg ataggtatgc gtggctatag
    26641 taatgaatcc atcacgccaa actattatct gaaagataac gtaggaggag ttatctacag
    26701 caaatatact tttgaggtca gatatccttt ttcgcttaac cccaatgcaa ccatctatgc
    26761 gcttagcttt cttgaggcag gaaactcgtg ggaaagtttt gctgctttta acccatacac
    26821 tgtttttcgt tcagccggat ttggcttaag ggtcttttta ccaatgtttg gaatccttgg
    26881 cctcgactgg ggctatggct tcgacaaagt acccggaata cccgatgcta acagaggaca
    26941 tttccacttt tcaatcaacc aatcaattga ttaatgactg accattaaat ttctttttgg
    27001 aatattattt gataaaactc gtgctaataa ttaaaacttt aaattaaaag ccatgaaaac
    27061 attaaaaatc actattcgag tattgattat cacaatcaca ctttcgctta caggcttcac
    27121 aaatacattt gcacaaaagt ttgcctatgt tgatagcgat tacattttaa aaagtattcc
    27181 cgaatatcaa aatgctcaaa acgaaataga tgagttatca aaaacgtggc aggccgaaat
    27241 agaagcgatg ttcacacaag ttgaacaaat gtacaaagcc tatcaggctg atgctgtatt
    27301 gctccctgaa gaccttaaaa agaaacgtga agatgagatc attaaaaccg aacgcgaagc
    27361 taaggagcta caacgcaaac gttttgggca agacggcgac ctgttcaaaa aaagacaaga
    27421 gttaataaaa cccattcaag aaaaaatttt taatgctatc gaagagatcg ccactaagca
    27481 aaattatgct ttcatttttg ataaagccag cggccctaca atcatgtaca ttaatgcccg
    27541 atataatatc agcgatgaag tcctcgatca gatagggagt attatgggtg taaggaaaca
    27601 ataaaggttt tttttaaaac aaattttatt tttttaaata atcagaactt caactgaata
    27661 gactactttt gcgccaaaca aaaattaact tgaaataata aagtaataag atgaacaaaa
    27721 cagtaaaatt aattgcattg gtggcattta tagtatttgc ctcctttaac tctgtacaag
    27781 cccaacaaaa atttggtcat gtgaatttcg ctcaggttta cgggttaatg cctggtcagg
    27841 attcaataaa aaaggtgtat gaaacctatg ccaaaggact tcagaaccag cttgctacga
    27901 tgcaggcaga attggagagt aaatacactg attatcaagc aaatcaggct acgtggtcaa
    27961 acataatccg tcagacaaaa gaaaaagaaa tccaggatat gcaaataaga ttagaagatt
    28021 tcaattccca agcgcaaatt gaccttagaa acaaagaaga cgaattgaca gccccactta
    28081 ttgagagggc tcgtaaagct gtagaagatg tagctaaaga gaatggttac acttatgtat
    28141 tcaacagtac tgaaggcttg ttgctttatg ccacaccaag cgatgatata atgtcattag
    28201 ttaagaaaaa attgggattg aaataaaaaa agccttcaca aaacggtttt ttttaacttt
    28261 attcattaaa aaccttcgat gcaaaagagt gaatcattct taaagtttca cctggcgctt
    28321 ctaaaaaccc catatgccct acatcagcca ataccaacac ttccgagtgc cttggtagta
    28381 tagcctgagc aagcacattt tgaacgggaa tccttgaatc ttctttacct gcaataaaaa
    28441 gtacaggaat atttatgttg agcaataaat ccaacttacc ggtacgctct ttcattccgt
    28501 aaagcgcagc aacaattcct tgagcgctgg ttttgtcggc aatattccaa agcatttcaa
    28561 tttcatcatg taactttact acattgatct cggcaaataa atcggggata aattgtttaa
    28621 caaacccttt tttatttaac ttgacaatag caatattccg gcggcggttt tctttcgact
    28681 cttcggtatc ggcagcggca tgggaatgaa aaagcactat acctttgagc atttcaggat
    28741 attgcttggc atattcaagg gtaacgtatc cacccatcga atggcctatc ataaggcatt
    28801 tctttacatt aagatgatca agtactgttt ttacagcttc tgccatcaac tccatcgtat
    28861 gtactttgtc tattgtgggc gttttaccat gccccggtag atcaacacga attactttaa
    28921 acgtttcctt tagctggctg gcaaagtaat tccatgccat ggacgatccc ataaatccat
    28981 gtaaaattac cagtggatat ccatcgccat ctaatttgta ataaatagct ttgtttttaa
    29041 aagcaatttt cttcattttt ttattaattt tctttatctc ttgacatcaa ggattcaatt
    29101 atttcgggtt ttattggaat atctgctgaa aggttaactg ggccattttc agttattaaa
    29161 atattatttt caagcctgat tccaatcgct tcactcctta tataaattcc cggttcacat
    29221 gttaatatca ttcccggttt aaaaggttcg tacttacata tcacatcatg tacatccagt
    29281 cctaaatgat gagctgtgcc atgcataaaa tattttttaa acaaaggatt gtccgggtct
    29341 tgttttttta cctcctcctc attcaaaagg ccaattttta ttatctctct ttccatttca
    29401 ttatttacaa aggtattata tttttccata gtgtttccaa ctatcatttg tttgatagca
    29461 cgttgttgta cctttaatac caagtcgtat aactgtcgtt ggcgcggttt aaatttccca
    29521 tttacaggta ttgttctcgt aatatcggca gcatagttag catattcagc gccaaaatca
    29581 ataagtaata actggccatc gtcaataata tcattattat cattatagtg aagtacgcat
    29641 gcattttttc cggctgcaat aattgggggg aatgcacttc cagtagaacg gtttataaga
    29701 aactcatgct ccatttcggc ttgaatttca aattcatgca ctccaggtcc gacaaatttt
    29761 aaaattctat aaaaagactt attggttatc tcaatagctt ttcgaattaa gttaatctct
    29821 gtttcagatt taaccatcct aagctttgtt aagagcgggg cggctcgttc gtaattatga
    29881 gccggatatt gatattgtaa ttggcgggca aacctatgat cgcgtgaatc aatttctgta
    29941 gagaatttgg gatactcata gatatttaga taaacatggt cgctccagat gatcaggtcg
    30001 cgcagagtaa gttccaaatc ggataaccat ttcacattag caatcccgga aatttgcatt
    30061 gctttttctc ttgtaagcct gtcaccatct ctgatagcgg tttgttcgtc agtttcaaca
    30121 agaaatagta tttgccttag tttaatatca ggatggttgg gtgcgattac taaaaccgac
    30181 ttctcctgat taattccagt gagataaaaa aaatcggaat tttgccgata aacatatgct
    30241 tgatctccgg aacgtatgaa ctcatcattt gaattaaaaa tagctaccga tgatggcttc
    30301 atcaatttgc caaacctttc acgattggct tcaaaaaatg atttatcaat gggttggtac
    30361 ctcatatctt acttattata atttaaaaga attataaatt actaatgaaa aacattttgt
    30421 ttgaaaaagt aaaagctttt aggtataaat ttgtccaatt acacttacaa aaaagccttt
    30481 ttctataaat atttaaaaac caaaaataat aaacttatgg gtaattctac atttcattta
    30541 gcctcaatca ccaaaaaagt cataatggct cttgtgggat tgtttttggt tatgtttctg
    30601 ctcgtgcatt taggtttaaa tcttacagtt ttaattttgg aaggtgatcg tgagattttt
    30661 aataaagctg cccactttat gggtactaac ctaattgtta aaatagtcga ggtgggtctt
    30721 ttcggatttt taattgttca tataataatt ggtatagtgc ttcaactgca aaactggggt
    30781 gctaggccaa aaagatacaa gaaaagagcc actacacaac tatcgttttt ttcgcgatac
    30841 atgattcata ccggagcatt gattttcatt ttcctgataa tccatttcag tgatttttat
    30901 tttaaagcaa aatttaccga tttagtgcca ttaattactt atgatggaaa agaatatcat
    30961 gatttgggcc tgttaaccct tcaaaaattc aaaatgggag gctttgtcat tggttatatc
    31021 attgcattta ttttattggg atttcatctc aaccacgctt tccaatccgc atttcaatca
    31081 atgggttgga atcattcccg atatatgcca tttattaaaa aagtagggct tgtgatctct
    31141 atccttatcc ctcttggatt taccattatt cctttggtaa tttatttttc tgaagcttat
    31201 tctgtttaaa atgaaaatat taataatatt atgataaaat ttgacgcaaa aattcctgaa
    31261 ggaccactaa ctgaaaagtg gagtatccac aaaagaagtg taaagttagt gaacccggct
    31321 aataagacaa aattagatat tatccttgta ggtactggat tggcaggtgc ggcggcagca
    31381 tcttcgcttg gcgagttagg ttataatgtg aaagcttttt gttttcaaga tactccccga
    31441 cgtgcacatt cagtagcagc tcaaggcggt atcaatgctg ctaagaacta tcgtaatgat
    31501 ggcgacagtg tttacaggct attttatgat gttatcaaag gtggcgactt tcgtgcccgt
    31561 gaagccaatg tatatcgagc tgctgaggtt agcaaccaaa ccattgatca gttggtagca
    31621 gccggcgtac catttgctcg tgaatacagt ggatatttag ataatcgttc gtttggcgga
    31681 gtacaggtat cacgcacctt ttatgctaaa gggcaaaccg gccaacagtg tttgctcggc
    31741 gcatattcag gaatgatgag acaagttgca aaaggtaaag taaccatgta ttcgcgccgc
    31801 gacatgctcg atcttgtagt tattgacggg aaagcacgtg gtattatcac tcgcaatctt
    31861 gttaccggag aaatagagcg acatgcaggt cactgtgtag tgttggcaac aggcggttac
    31921 ggcactatat actatctgtc aactcttgcg gtaggatcaa acgtctcggc agcatgggca
    31981 gcacataaaa aaggagcgct ctttgccaat cctagtttta tacaaataca tcccaccagc
    32041 attccgcagt tgaacgacta tcaggctaag cttacactta tgtccgaatc attgagaaac
    32101 gacggccgta tatgggttcc aaagaaaaaa ggcgacgcca gggctgccaa tgatattccc
    32161 gaagacgaac gcgactatta tcttgaacgt cgttatccgg cattcggaaa cctcgttccg
    32221 cgcgatgttg cttcgcgggc agcaaaagag cgctgcgatg caggatacgg agttgggaaa
    32281 accggcttat cagtatatct tgattttaaa gacgcaataa aaaagcaagg aaaaaaagct
    32341 attgaaaaca aatatgggaa tctctttgag ttgtattata aaataactgg cgacaatccg
    32401 tatgaggtac ctatgcgtat ttatcctgcc gggcattaca caatgggcgg cctttgggtt
    32461 gactataact tgatgaccac catcgaaggg ttatatgcaa taggcgaagc caacttctcc
    32521 gatcatggag ccaacagatt gggggctggc tcactgatgc aatgctcggg cgagggctat
    32581 tttatcctgc cggttaccat tggtgattat cttgctgatg agattaaaac cccgcgaata
    32641 ttaaccgacc atcccgaatt tgaaaaggcg gaaaaagaag ttaaagagaa gatagataag
    32701 ttaatggcta tcaatggaaa gcagacgcct actacttttc acaaaaggct tggtaaaatc
    32761 atgtgggatt attgcggaat ggctagaaat gaaaaaggtt tgcagaaagc aatgatcatg
    32821 gtacaagagc ttgaagaaga attttggaaa gacctcaaag tgtcagatag tgcaaaagaa
    32881 ttgaatcagg aattagaaaa agcaggtcgt gtagttgatt tcttcggtat agctaaactt
    32941 atgattcaag atgcgttaaa ccgcaaggaa tcgtgcggcg ctcattttcg cgaagagagc
    33001 caaactcctg atggcgaagc aaaacgtatt gactccgaat atagttatgt agcaatttgg
    33061 gaatatttag gcgaagataa gcctcacact ctacacaaag aacctttagt tttcgaaaat
    33121 gtggagttaa aagagagaag ttataaataa tcacgcattt aacaccctaa tgatatgaat
    33181 attacacttg aaatatggcg gcaaaaagat gccaaaagca aaggaaaatt tgtcatttat
    33241 aaacttaacg atgtttctga agagatgtcg ttcttagaaa tgctcgattt tctaaataat
    33301 acccttattt caaaaggaga gagccctgta gcttttgatc acgattgtcg ggagggtatt
    33361 tgtggaagtt gcggtcttta tattaatggc tgggcacatg ggccggcgca cctgactacc
    33421 acttgtgaa
//
LOCUS       DAOOAI010000011        33195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_1711_length_33195_cov_5.337598, whole genome shotgun sequence.
ACCESSION   DAOOAI010000011 DAOOAI010000000
VERSION     DAOOAI010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 33195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33195
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1711_length_33195_cov_5.337598"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..606
                     /locus_tag="PK345_01710"
     CDS             <1..606
                     /locus_tag="PK345_01710"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPX33770.1"
                     /translation="INHRFANQPIFDREQDFDIYCFIDSVLMKKYPDNSHVLEHHNRV
                     SEIKERLTDQRVIEESLSAGRPVPDIILNDADGVSFRLSDLKGRAVLVYFWVSWSPLC
                     RAANHQLKDIYNDYRDSGFEIYAVSLDHQARYWKDAVRLDGLTWINVSDLNGISSPVV
                     TLFNIPQDLPYYYFCNEEGAIVAKGKKFGEIKKAIAEYFKH"
     gene            714..1463
                     /locus_tag="PK345_01715"
     CDS             714..1463
                     /locus_tag="PK345_01715"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008758 - UDP-2,3-diacylglucosamine
                     hydrolase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HPX33771.1"
                     /translation="MIYFASDLHFGIPDRKSSAQREEKFIRWLDLIKNDAVEIFLMGD
                     LFDFWFEYKTVVPKGYVRLLGKLAEITDSGIPIHFFKGNHDMWAFDYLEKEIGMTMYR
                     QPIERVIGGKRFFLAHGDGLGPGDRGYKFLKNVFELKINQFLFRWLHPDIGVWLGLYW
                     SAKSRYANLIKEAERGEEYDFQNARIAVYCRDILSQGRQIDYFVFGHWHVVNMEKIGD
                     NSYYIHLGDWLTNFSYGVFDGNTFELKRFDN"
     gene            complement(1469..2257)
                     /locus_tag="PK345_01720"
     CDS             complement(1469..2257)
                     /locus_tag="PK345_01720"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPX33772.1"
                     /translation="MNDFRKNAIVTGATGAIGTAIARQLAIKGFDVTIIARNEQKALK
                     TVNEIQTSSKNPNVSFLLADLSVKSSIQQLASNWERPLHVLINNAAVTPRTREETNEG
                     IELQWATNVLGYFWMINCFTSFLKMAAPARVVNVASYWAGDLDISDLECKRRRYDNDM
                     IYRQSKQANRMLTIVFAEKLKPFGITVNAVHPGDVNSTLSNNLGYGGFQTPDQGADTP
                     VWAATQPELTDITGKYFERREETTCQFASNKPLAYKLFEICERY"
     gene            2449..3414
                     /locus_tag="PK345_01725"
     CDS             2449..3414
                     /locus_tag="PK345_01725"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HPX33773.1"
                     /translation="MLTQYALIFSIALHFGAAIIVISLIRATKYNSSWILLSIALFVM
                     AVRRVIEYFSYMDKAISDNILMLNSWLGVIISLLMLVGMFYVKKVLNYLFKLEEVRSA
                     ADKRLLNTIIRTEESERHRLAKELHDGLGPLLSSAKLLTSALNESQTGGHEILKRMIS
                     TIDESIYTLKEISNNLSPHMLENFGLPTAIQSFIDNLEQTSKLRFEFRTNIIDQRYGG
                     NMEIVLFRTVCELIHNTVKHADANKVLISLDEENDQLKLLYQDDGIGFDQEKLLMADN
                     KGMGIGNIRSRISSLGGKFEIESWPDEGIIVTVELKIQDENKIPI"
     gene            3570..4220
                     /locus_tag="PK345_01730"
     CDS             3570..4220
                     /locus_tag="PK345_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019152095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX33774.1"
                     /translation="MKSRKFTLSIVDDHKLFRNGLRLLLVNSYPNIEITEANDGNEYL
                     QSLATHLPDVTLMDISMPIIDGIEATRRATELYPDIRIIALSMFGDEAYYYKMIDAGV
                     KGFLLKNSEIAEVREAIESVMAGDHYFSNELLLSIVKNLNKKSLEEDTGINLSERETE
                     ILQLICLGLSNKEISEKLFLSKRTVDKHRANILFKTNSRNTAHLVMNTIKHKWIVF"
     gene            4469..6163
                     /locus_tag="PK345_01735"
     CDS             4469..6163
                     /locus_tag="PK345_01735"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPX33775.1"
                     /translation="MKNKRIIVIGGSAAGPKAASRARRLDENAEIIMIQKASDMSMAS
                     CGYPYYVGGFFDDRDQLLCSPAGVIRDSKFFWNAKKIVTHVNTEVTSINRKAKKVEYE
                     NLLTGEKGTLEYDKLIIATGAVPRKPQIPGADLDGITTLQSLSDADYLRKVGEEGKIK
                     KAVVIGGGLIGIETMEALNLAGIEITLIELLPQLLTFLDKHLARLIEKYIQTKANVIL
                     RNGVASFLGENGKLKAVKLNDGTEIPCDLAVVAIGVIPNSKLAKDAGLEISPTGGIVV
                     NGYMQTSDPDIYAVGDCVEIPNLITQKPVHAPFGDLANLQGRVAGENVILGNVAKYPG
                     TIQTGVCKVFDYGVGSTGLSEQNAIKLGFNNIETVVNTSLDKPGFMKGQLLITKLVVD
                     KSSGLILGAQVIGPGDVSKQLAIWAIAIKGKFTVEDMVNADLPYAPPFSLAIDHSITT
                     AHIMQNKLRGLFKGISSEELKNKLNNKEPIHLIDVRNPNEFEQMRLGIGEKLIPLGQL
                     RSRINELPEDKNTEIVVWCKISLRGYEASLVIKANGYKNVKVLEGGIMAWPWSREK"
     gene            complement(6184..>11661)
                     /gene="sprA"
                     /locus_tag="PK345_01740"
     CDS             complement(6184..>11661)
                     /gene="sprA"
                     /locus_tag="PK345_01740"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPX33776.1"
                     /translation="AATQGGTIQSTNGKVYFTVLEPFGSYLRAKIGDDDIADRFVYDS
                     LYTLTKTGAQQYPEKNKFMLEGFYKSSSGSEISLNAINVPQGSVKVTAGGAPLMENVD
                     YTVDYTLGRVRIINEGVLNSGTPINISLESQSFFSVQTKRMMGTHIDYRINENFNIGG
                     TLLNLTERPLTQKVSYGNDPISNTIWGMDVAYQTQSRLITKIVDKLPFIAAKDESRIN
                     IDGEFAHFVPGHAKVIGSTGTSYIDDFEAAKSTIDLKNMAYWQLSSTPQKQTTADMFP
                     EAAPSTGLAYGFNRAKLAFYVIDPLFYDETGSHRPSNINLNELSDHQVRQVLETEVFP
                     NKDVPNGVPTNIPILNLAYYPSERGPYNYDVEGEPGISAGVNADGLLNLPETRWGGIM
                     RKIESTNFDATNVEYIEFWLMDPFVKGADNSGELYFNLGDISEDILRDGRKSFEHGLP
                     PTEEVENVDTTIWGRVPSFQALVDNFSNVPGSRAFQDVGLDGLRTEDERSFFQTQFLE
                     PLAALYGSESEAYKQAILDPSSDNYHYFRGSDYDKDEKYSSILERYKNFNGPDGNSPT
                     EDINPESYPTAATTRPDAEDINRDNTLSDDERYFQYKVLIDPNKMNVGENYITDIYNA
                     QGIRLPNGQIGEVKWYQFKIPVRDPEKIIGGIQDFQSIRFLRMFLKGFENPIVLRFAT
                     LDLVRGEWRRYRYDLLAPGEYIPNDIQSETSFDILSVNIEENGKRQPIPYAIPPGIER
                     EVNLGTTNLQQLNEQSMVLRVCNLIDGDARGAYKTTDFDFRDYGFIEMFVHAEKLIDN
                     QELNHGDLTVFMRFGADLTENYYEYEIPLHFTPWHTSYNEPDLIWPEENKFKIDLKRL
                     VQFKIERNEQMRNAGATGISLTMPFYAFDGNNRVTILGSPNLSSVKAIMIGVRNPKQT
                     SFSINDDGEPKCAEIWVNELRLTDFNNQGGFAATARLRSDLSDFGNITLTGLFSTPGF
                     GTIEKKTSERSKEFIKNWDFSSNLELGKFFPENAGIKIPMHFDYSESRTDPKYNLLDP
                     DVLLREDVKTFDTKQDRDSIIRINQDYVQRKNINFINMRKTKMGASQKTNFYDIENWG
                     FTYSYSEIYARNVDYQYDIQKKYLGGIGYNFNTVPKNYRPFSSIKFFSPKPLTFIKDF
                     NFYLLPKLFSFRTDMIREYNKRLLRNKSQYAVLIEPTYLKKWDWTRNYSLNWDLTSSL
                     RIDYKADVGSYIDELPGSIEKDDPDYKIKKERIKTEIKNWGSKTLYNQMTGINYTLPI
                     SKLPLLDWVTANARYQVNYRWQASPKSLQARFGNQIENSSNIQLNGSLSFDRIYDKIP
                     GLKKINQPRSPQRAGGMTRPGMQQQQQKKSEQDTVKKERPDYFRIAGNTFLRLLTGFK
                     RASLAYSQGNGILLPGFVPEAGYVGNDWGQNAPGMGFIFGSQKDIRPKAVANNWLTMD
                     TLLNSAYITKFNEQINFRGNFEPFADFKIELNADRNYSLNHQEYFKADANGTFASYSP
                     QEQGNFSVSVLTWGTAWAKDNDKDQNENFERLKENRKEIAFRLANANPNWSKTKVDST
                     GFPEGYSSSQQEVLLYSFLAAYTGKEAANIKLNTMPAIPMLNWRLTYNGLARLEPFKK
                     FLRTLTITHGYRSNYSVGSFQTNLLYREFNGGASAFDDNHNFIHEHLIAQVSITEQFM
                     PLISFDMNWINSLMSKFEIKKTRSLSLGFVNNQLTEINSNEYVIGLGYRFKEVQFSIR
                     STTGGSQGSRKTVKSDLNVRADFSLKTDKTVLRRIDEDLNQVSAGQKVTSLNLSADYT
                     VNQRLNLRIFFDKVTNNPFVSSQYRTSTTKAGITLRFTLAQ"
     assembly_gap    11662..11671
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11672..13434)
                     /gene="sprA"
                     /locus_tag="PK345_01745"
     CDS             complement(<11672..13434)
                     /gene="sprA"
                     /locus_tag="PK345_01745"
                     /inference="COORDINATES: protein motif:HMM:TIGR04189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell surface protein SprA"
                     /protein_id="HPX33777.1"
                     /translation="MIRRLTYILLFALLALFSLAAINRSTVKETNKHVLNPDPFRFLR
                     PDTIELIYPFANPTSVTESKPESPLFLKEPSNVQHQVEYDPETNEYTIKEKMGKLNYR
                     TPYSMTATDYMKWDQERALQQYWRERAISAGQGASGTGLIPQIYIGGEIFEKIFGSGT
                     IDIRPQGSAELTFGVLANKREDPTLSVRQQNIVNFDFQQRIQMSVLAKIGDKIEFQTN
                     YNTEASFEFENKLNLKYEGKEDEIIQLIEAGNVTLPLNSTLITGSQALFGIKTKLKFG
                     RATVTTVYSEQKSETQSITVQGGAQTNAFLLNSDQYEENKHYLLAQYFRETYDTALSE
                     LPVIRSNVNITKIEVWVTNIGAAVTDNRNIIALQDLGEKNPYSPNVTGKPSVFPDNYR
                     SNNLMEMLLSFEPDIRDINKVSQIITDAPFYFEAGVDAVKVESARKLSPNEYTFNGKL
                     GFISLNTTLNPDQVLAVAFQYNVIGDTTLYQVGEFSDGGINSPKNLIVKLLKSNSLST
                     RVPIWNLMMKNVYPIGAYQIQSKDFILNILYSGNQNAVPTSYLTDAGNASGIPLIRVL
                     NFDNLDPQLNPPYDGIFDFID"
     gene            complement(13569..14165)
                     /gene="ruvA"
                     /locus_tag="PK345_01750"
     CDS             complement(13569..14165)
                     /gene="ruvA"
                     /locus_tag="PK345_01750"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HPX33778.1"
                     /translation="MIEYFSGKLVEKNPAYVILEQNGTGYLINISLNTFSSIKDLDEA
                     KLYVHLTFKIEATTPTGIVVYGFFDQAERSLFRMLISVSGVGSSTAMRMLSSLSYQKI
                     TETIRAGELATLKGIKGIGNKTAQRIIVELQDKIGKEALAPEFLGWTHNTQKEEALIG
                     LTTLGFGKHASEKALNSILKKNPNLSVEELIKEALKIL"
     gene            14392..16050
                     /locus_tag="PK345_01755"
     CDS             14392..16050
                     /locus_tag="PK345_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33779.1"
                     /translation="MKKSYLLLFYSIFAVFIHFSAVAQISEQGTPPSIEYSGSLSHHP
                     VVTVPPPDIELLKSEDGFYQKNAIAYRFAESIPVKIDFAKEGLWESLPDGRRICRLSI
                     KSPGAQALILYYSRFKIPAGGNLFLYSADKKQIIGAFSSNTNSADKSFATEMIKGEEV
                     ILEYSEPFALSVKPQIVINEVGYVYRTADRFFNMRGFGTSDTCEVNVNCPEGEEWQYQ
                     KNGVVRIIVKSGFSSFWCSGSIVNNARNDCTPFLLTADHCGPHASEENYDQWVFYFRY
                     EGDDCDDPESDSTFKSYTIVGAKKLAGNGGAAGKASDFKLLKLNENIPVTYNPFYNGW
                     SIANEPSPSGTSIHHPDGDIKKISTYTDTLKSSNWGTIPNTHWRVIWAQTETNWGVTE
                     GGSSGSPLFDNNGRIIGQLTGGEASCKYVYKPDYYGKFSYSWDGVPTADSTMLKPWLD
                     PDDTGITEIDGIVSINEPIKRSSALYLYPNPAEEAIYIRDERITGNPVQVIITNLHGQ
                     EVLIEHFPERADNMINLNVNTLKKGIYIVYIIINDELRTAKLIR"
     gene            complement(16232..18061)
                     /locus_tag="PK345_01760"
     CDS             complement(16232..18061)
                     /locus_tag="PK345_01760"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018074650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPX33780.1"
                     /translation="MAQNQIPLEDFFRNPEKTGYQISPDGKFYSYLAPYENRLNIFVQ
                     QIGKEKSIRLTNETDRDIAGYYWANKNRILYLKDKGGDENFALYGVDRDGKNLKCLTC
                     FEGVRTMIVDDLKDFPDEVIISLNKRTPVVFDPYRLNIKTGEMTMLAENPGNIQGWMT
                     DHEGKLRIAIAIVDGVNTQILYRDSEDQEFRPVLTTNFKESVSPYFFDFDNKNVYATS
                     NLGRDKSVVVLFDIANGKEIEALYEHPDYDISSVSFSRKRKVVTSASFVSWKRERHFF
                     DQQTKTLFENLQKQLPNYEIGISSLDDNENMYIIRSYSDRSLGAYYLYDFAKNKLTKI
                     HDISPWLDESKMAQVFPIEYEARDGLVIPGYLTLPNGLTMETAHNLPVVINPHGGPWA
                     RDSWGFNPEVQFLANRGYAVLQMNFRGSTGYGRDFWERSFKQWGLTMQNDITDGVYWL
                     INKGIADKDRIAIYGGSYGGYATLQGVTVTPTLYAAAVNYVGVSNLFTFLKTIPPYWQ
                     PLLDMMYEMVGNPEIDTVQFRATSPALNAHKIITPLFVAQGANDPRVNKAESDQIVEA
                     LNKRGIEVEYMVKDNEGHGFANEENRFDFYRAMEKFLAKHIKK"
     gene            complement(18233..19309)
                     /gene="corA"
                     /locus_tag="PK345_01765"
     CDS             complement(18233..19309)
                     /gene="corA"
                     /locus_tag="PK345_01765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HPX33781.1"
                     /translation="MKIPQSKIFKLMKTKAGAPPGTLINTFAQTSVHESTRLISVSDD
                     SNQDIIIEDFEKLPSRLWPEATNWLIFTGLGDIDKLEHLGSVFQINPLIIEDILNAEH
                     LPKAEETDEFLFFVIKILTRNTETDSFETNHVCFLLGKRFIITFLQRNTSLFDNFIKR
                     IKTVKESPHHYTHDYILYQLIDIIVDHYYLLFNQTDEKLEEIEEILMSDQSADMTSMI
                     QQQKRELNFIRRNILPVAEAVRILLKIESAFIKKHNRTFFNDIADHLQHLLSATDNYR
                     ETITSLYELQMMNYSNRMNNVMKSLTIIATIFIPLTLITGIYGMNFTYMPELSLRWSY
                     PAVLIFIVAGGTAMYLYMRQKKWF"
     gene            19410..20939
                     /locus_tag="PK345_01770"
     CDS             19410..20939
                     /locus_tag="PK345_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPX33782.1"
                     /translation="MVKFNIYARKKQWKLFLFALAVAIVSGSMYYTSIIVRKVAEQEQ
                     TNVKIWADAIYRKTELVKYIEQFFQQIREEDRKSVVLLAEAYKQLIRTDDPNVDLTFY
                     VDIIRYNSTIPIILTDEDGRIINTKNVDFNEDTVTFLSGKLLDEFSVYPPIPARYFQN
                     KKNYLYYKDSKLHSELHQVLNDLVSDFFSEVVINAASVPVIITDSTKQKVIDFGNIEP
                     NKIMEPLFVEKTIAEMAASNPPIEIELTNLGKSYIFYKGSSLLTQLRYFPIVQILIMG
                     LFLFIGYMMFNTARRSEQNQVWVGLAKETAHQLGTPLSSMMAWMELLKEENPNPEIVS
                     ELRKDVERLEKVTDRFSKIGALPKLTDTNIVKVIHNSIEYLKTRTSRKITYEITPGPD
                     TEIIAPINLHLFEWVIENLTKNAVDAMESKGTFSVNIEENNGHIIIDLTDTGKGIPKS
                     KFKSIFNPGYSGKKRGWGLGLSLAQRIIRDYHRGKIFVKSSTIDKGTTFRIILRKKVK
                     L"
     gene            20946..22073
                     /gene="hemW"
                     /locus_tag="PK345_01775"
     CDS             20946..22073
                     /gene="hemW"
                     /locus_tag="PK345_01775"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HPX33783.1"
                     /translation="MPGLYIHIPFCRQKCHYCNFFSIASKKYINEIVQAIQTEVRSNS
                     GFFNGSPLNTIYFGGGTPSLLSINQINEIIDTAIATFGIDPNAEITVEANPDDLINSW
                     LQALSQTVVNRLSIGVQSFRNEDLTYLNRVHSANQAESAIQNALKYGFKNISIDLIYA
                     MPTLDNEQWIENINRALSYNIPHISAYALTVEPGTALDLFIKKGKYKQVEEEKVAEQF
                     QIIMTSMAKSGYEHYEISNFALPDRYSRHNKSYWSRESYLGCGPSAHSYNGFIRRWNV
                     SNISNYLKAVREGTTYYELEELTKNQQFNEYIMTALRTMWGVDTEFVKKLFGNDFATH
                     LNNQLKKPLELGLILQKNQKFILSDQGKLFADGIAAELFIS"
     gene            complement(22214..23377)
                     /locus_tag="PK345_01780"
     CDS             complement(22214..23377)
                     /locus_tag="PK345_01780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33784.1"
                     /translation="MKRNLTIILIAIMFPLSIFSQISNEIATYVDSTELLVHNGRKMI
                     LKELADNNLVKTKEIYDFLTEKTKDKPFSAFYFIEDFYINMLVGDWETVNNLMLEYEK
                     HKKRIVYPNSHTIVYKLSELTLSNRQSILVSCNMSQIDEQAKLLITGFLKYIEKQPPD
                     KEYNEILVAYKKKYDNKKYDSFVNGFMPHMIIKASWNLFFGSGMVFTTGDLATKFSNN
                     ALFNFGMDINVHRVFASLYLKGTSLKLKDPFMVTSDTDTLNFELNEKFSYLDAGLKGG
                     YFIVRSNRFHLAPYASISGSHLESTKYDDPKDKDLEYEIFNSFTYGIGLHTEVKIYEF
                     ESKNYYYYGGTANGHISIKFETGYNEILKFKDLNSTGNTPYFLCSLVIGFGQF"
     gene            complement(24047..24721)
                     /locus_tag="PK345_01785"
     CDS             complement(24047..24721)
                     /locus_tag="PK345_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPX33785.1"
                     /translation="MKNVIKLLIFVLVVSMTTGAFAQNFGLKGGLNLSNILAKDDNTT
                     YSDDFKMNPGFHIGATAEFPFSDMFSFETGLLLSTKGYKISEEETFMGKKIEMKIKTN
                     LFYLDIPLTAKASFDLGDAKVFGLFGPYIGMGLTGQSKTVTTIDGKTEKEKEDVEWGS
                     EKGKSDLKRLDFGLTIGAGVEIDLFQIGLGYNLGLANISPYNDGGMKINNRVIGLSVG
                     YKFGGK"
     gene            complement(25296..27143)
                     /locus_tag="PK345_01790"
     CDS             complement(25296..27143)
                     /locus_tag="PK345_01790"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HPX33786.1"
                     /translation="MSFEYGEDQIRTLDWKEHIQTRPGMYIGKLGDGSSQDDGIYVLI
                     KEIIDNSIDEFVMGNGRRIDIDISDKTVTIRDYGRGIPLGKVVECVSKINTGAKYDST
                     AFKKSVGLNGVGSKVVNALSSYFKVQSVRDGKLKIAEFSKGEIINDYAESPTDEKNGT
                     LVTFTPDISFFGNFHFIPEYVEKLLWNYVFLNNGLSIYYNGERFFAKNGLHDLLERYT
                     NGEALYPIIHITEGDLEFAFTHISNQWGEEYHSFVNGQNTTQGGTHLAAFREAIVKTI
                     REFFKKDFEPVDVRTSIAAALSIKIQEPVFESQTKTKLGSQYIEPGGQTVRNYVIDIV
                     KRNLDNTLHINPQTAEIIYQKILSSEKERKEIAGIKRLAKESVKKVSLHNKKLRDCRI
                     HYNQNHEQRLETTLFITEGDSASGSITKSRDVNTQAVFSLKGKPLNSYGMSKKFVYQN
                     EEFNLLQSALNIDEDLENLRYNNIVIATDADVDGMHIRLLLLTFFLQFYPDLVKSGHL
                     YILQTPLFRVRNKKETIYCYDDDERKAAIKKLGPNPEITRFKGLGEISPDEFKHFIGA
                     SMRLDPVLLRKGQSIKEMLEFYMGKNTLERQKFIIKNLRPSEIMLESTV"
     gene            27370..29823
                     /locus_tag="PK345_01795"
     CDS             27370..29823
                     /locus_tag="PK345_01795"
                     /inference="COORDINATES: protein motif:HMM:NF013239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 31 protein"
                     /protein_id="HPX33787.1"
                     /translation="MKSKVFTISLLFFCFTALGQNNLNVAFGNLVKLQKNPAKQEFTL
                     ISDNGTAKLTVYSPNIIRIRIAKEFNSDFSYSVVANPMKCNFDYGEDNNNFFVSTDSL
                     FLEISKKPIRFSFKTLDGQVVNQDDPAFGVSWIRNEVTNYKLIQGDEKFIGLGEKTGN
                     LNRRGEAYEHWNTDNPHYDNNSDPLYASIPFYIGIHSGLNYGIFLDNTHRTTFNFGAS
                     NDRFSYFSAEDGEMNYYFIYHKNVTDIISSFTWLTGRMEMPPVWSLGYQQCRWSYTPE
                     SEVMRIANTFREKKIPADVIYLDIHHMDNYKIFTWNPVDFPQPKKMIKSLAEMNFKTA
                     IIVDPGIKVEKGYMAYDEGVAYKHFVKYPDGSNWTAQVWPGWCHFPDFTSEKSRKWWG
                     EKFKSYVEDGVVGFWNDMNEIATWGQQVPNLIEFDWDGNKATYRQAKNVYGMLMARST
                     FEGVKKLMNGKRLMNITRSGFAGMQRYTILWTGDNQATDHHMMLGVRLVNSLGLSGLS
                     FTGSDVGGFGGNCTPALFARWMQIGAFTPFFRGHSAYNTISQEPWVFGETVEEISKNY
                     IQLRYNLLPYIYSSMHSSVISGIPLNRSLAINYTMDETIYQEEYQNQYLFGPSMLVIP
                     VESNKEIVKAYLPKGEWYDFYTDQYFDGQQEVFAESPQDKLPVYVLAGSIIPMQSPIQ
                     HTAEMPSDTLYVHLYKSQSNKDIAWKYYEDDGVSYDYQNGKFYLREIIYKPEINEVEF
                     RKPDGDFQSKFKNVRLVFHGFDELDWIIKFDGKPRKVYPLSMNFRKVSEIPPEGDYST
                     ITCPTIVFPNDEERMIITW"
     gene            29933..30769
                     /gene="nadC"
                     /locus_tag="PK345_01800"
     CDS             29933..30769
                     /gene="nadC"
                     /locus_tag="PK345_01800"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HPX33788.1"
                     /translation="MTIDEIIKAALAEDVGNGDHTSLSTIPQWAIGRANLLIKENGIL
                     CGVDIARKVFNTVDPDLDFQVFIEDGNDVVPGDIVFSVSGKVISILTGERLALNFMQR
                     LSGIATNTRLMVNELKGTKARLLDTRKTTPNFRELEKMAVRTGGGENHRMGLYDMILI
                     KDNHVDFAGGIKQAIKAVHNYIKDKALDLKIEIEVRNFDELNLVMETGGVDRIMLDNF
                     NVSDLKKAVELVAGRFETEASGNITLKNIREYAKTGVDFISSGQLTHQIRSLDMSLKA
                     DF"
     gene            30785..31744
                     /locus_tag="PK345_01805"
     CDS             30785..31744
                     /locus_tag="PK345_01805"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HPX33789.1"
                     /translation="MLDHKNKLLSKLNPKVVKFLRNSLAFIIRILRKITIPGTNKMPL
                     YDVMIFFLKGLTEGRLTLRASSISFDFFLALFPVIIVFFTLIPFIPITGFQEELLHFL
                     EDVIPSTLWIHVSETLEDIIVQPRSGLLSLGFLLALFFSTNGVNSIIEGFNSSYHTTE
                     KRPYLKQRLVSLYIVLVQSVLVILAVSLFLIGGRILQFLVFHGILTNQLTIVILHIVR
                     WLMIVGLFLVSVSFLYYFAPAKKKEYKFFSIGAIFTSGMMILTTYGFNFYIENFNRYN
                     ALYGSIGTLLVFLLWLYFNSIIILIGFELNISINTAKKRFENA"
     gene            31737..33149
                     /locus_tag="PK345_01810"
     CDS             31737..33149
                     /locus_tag="PK345_01810"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPX33790.1"
                     /translation="MLNHLRNTVKHTAIYSLGNLSTKIIGLILLPLYTSYLTTAEYGI
                     FSILEVTSQFFTAVVSLGLTAGMMRWYAVEDDEQKRKIIVFTTWVTTFILALFVNVAF
                     YPANKWFSSIFFSHDNYADYFLILWLWVGLEVVNRIPFELLRLKEKSLLFVTLVIIKF
                     VLILVLNIYFIVYMKMGVKGIILSQVIGNLLVSIFTLPFLLRNIFLKFSLTILKEISL
                     FSIPLVLSSVATMAFALSDRYLISYYLNYSEVGIYSLGYKVASVINLFLVQSFLLGFA
                     PLAYKIFDKPEAKRYFARVTTYFTFAMIALALLLIFYAKEVIALFSSGNKDFVLSFNV
                     IPLLCVAMIFRGLTAIVSMGLHYVKKTKYNLIIVVIVAVFNIGINFILIPLFGIYGAA
                     VASILANMLMTILFYVYSQRYYFISYEFVRIIKLFLFGIVFLFLASFATVFGFWGGII
                     FKTFLLLLFPLALTIVKFYQ"
BASE COUNT         9762 a         6296 c         6601 g        10526 t
ORIGIN      
        1 ataaaccatc gctttgcaaa tcagcctatt ttcgaccgcg agcaggattt tgatatttat
       61 tgttttattg atagtgtttt gatgaagaaa tatcctgaca acagtcatgt tttggagcat
      121 cacaatagag tatcggaaat aaaagaacgc cttacagatc agcgggttat tgaagaaagc
      181 ttaagcgctg gccggcctgt tccggatatt attttaaatg atgctgatgg ggtttcgttt
      241 aggctctctg atttgaaagg cagggcagta ttagtatatt tttgggtatc gtggtcgccg
      301 ctctgccggg cagcaaatca ccagcttaaa gatatttaca atgattatag ggattcgggt
      361 tttgagatct atgccgtctc cctcgatcat caggcgcgtt actggaaaga tgctgtcagg
      421 ttggatggat taacatggat caacgtttct gatctcaatg gaatcagctc gccggttgta
      481 actcttttca acatccccca agatttgcca tattactatt tttgtaacga agagggcgcg
      541 attgtggcta agggtaaaaa attcggagaa ataaaaaaag ctattgctga gtactttaaa
      601 cattgataat aaaccgaaga tttccgaaaa agagggtggt aaagttcagt atctctgtat
      661 atgcttattt ttaccgaaag ttttgaaaac agggttgtta acttcctata aaaatgatat
      721 attttgcttc tgatttacat tttggaatcc ccgacaggaa aagtagcgca caacgggaag
      781 agaagtttat ccgttggctt gatttgataa aaaatgatgc tgtcgagatt tttttaatgg
      841 gcgatctttt tgatttttgg ttcgaataca aaactgttgt accaaaaggg tatgtcaggc
      901 ttctgggtaa gcttgccgaa ataaccgact ccggtattcc aatccatttt tttaaaggca
      961 atcatgatat gtgggcattc gattatctgg aaaaggaaat aggtatgaca atgtatcgcc
     1021 agccaataga gagggtgatt ggcgggaagc gttttttttt ggcacatggc gatggattag
     1081 gccccggcga taggggatac aaatttttaa aaaatgtttt tgaattaaag ataaatcaat
     1141 ttttgttcag gtggctacac cctgatatag gagtgtggtt aggcctctat tggtcggcaa
     1201 aaagccgata tgcgaatctg ataaaagagg ccgaaagggg ggaggaatat gattttcaaa
     1261 atgcaaggat tgctgtgtat tgcagggata tactctcaca aggccggcag attgattatt
     1321 tcgttttcgg acattggcat gtggtaaata tggaaaagat cggtgataac agctactaca
     1381 tccacctggg cgattggctc acaaacttct cttatggtgt ttttgacgga aatacttttg
     1441 agttaaaaag atttgataac taaccgtttt aatatctttc gcaaatctca aaaagcttgt
     1501 atgccaaagg tttgttggat gcaaactgac aagtagtctc ttctcttcgt tcaaaatact
     1561 tccctgttat gtctgtcagt tctggttgtg tggcagccca tacgggggtg tcggcgccct
     1621 gatccggcgt ttggaagcct ccatatccaa ggttgttgct caaggtcgag tttacatcac
     1681 cgggatgaac agcatttaca gtaataccaa aaggctttag tttttcagca aaaacaatag
     1741 tgagcatcct gtttgcctgt ttgctttgac gatagatcat atcgttgtca tatcgccgtc
     1801 ttttacactc caaatcggaa atgtcaaggt cgccggccca ataactggct acgtttacta
     1861 cccttgccgg cgctgccatt ttaagaaatg acgtaaaaca gttgatcatc cagaaatagc
     1921 ccaaaacatt tgtagcccat tgtaactcga tgccctcatt agtttcttcg cgtgtgcggg
     1981 gtgtaacggc agcattgttg ataagcacat gaagcggtcg ctcccagtta ctagccaact
     2041 gttgtataga ggactttact gatagatcgg caagtaaaaa gcttacatta gggtttttac
     2101 tgcttgtttg aatctcattc acggttttta atgccttttg ctcgtttctg gcaattattg
     2161 ttacatcaaa acccttgata gctaactgtc tggcaatagc tgtaccgata gcgccggtag
     2221 cgcctgttac gattgcattt tttctaaaat cattcatgcc gcaaaataaa cttattatgt
     2281 tgacttgtta ataatctcca tcttgaaaga taatatcaga aatcgtctct ccaatagtgt
     2341 aaatgtttca gggcattttt tttaaaatat tgctttcaaa caatattaat gaacttgtca
     2401 aacttatgtg tctggcattt ttgtatcttt acatcaaaaa ttactgctat gcttacacaa
     2461 tatgctctta ttttttccat tgctctccat tttggcgctg caattattgt tattagcctg
     2521 attagagcca caaagtataa ttcgagctgg attttgcttt cgatagctct ttttgtaatg
     2581 gctgtaaggc gtgtgattga atacttttcg tacatggata aggctatctc cgataacata
     2641 ctgatgctca atagctggct tggggtaata atatcgttac taatgttggt ggggatgttt
     2701 tatgtaaaaa aggtgcttaa ttatcttttt aagcttgagg aggtgcgttc ggctgccgac
     2761 aaacgtctgc ttaacactat tatccgcacc gaggaaagcg aacggcaccg gttggccaaa
     2821 gagctgcacg atggacttgg ccccttgctc tcgtcagcaa aactgttaac atcggcatta
     2881 aatgaaagcc agaccggcgg acacgaaata cttaaaagga tgatctccac aatagatgag
     2941 tcaatatata ctcttaaaga gatttctaac aatctgagtc ctcacatgct tgaaaacttc
     3001 ggacttccca ctgcaataca atcgtttatt gacaatttgg agcaaaccag taagctgcgt
     3061 tttgaattca gaacaaatat cattgatcag agatatggtg gtaacatgga aatagtgttg
     3121 ttcagaacgg tatgcgaact gatacacaac acggtgaaac atgcagatgc taacaaagtt
     3181 ttgataagtt tagatgaaga aaatgatcaa ctgaaattac tatatcaaga cgatgggatt
     3241 ggctttgatc aggaaaagct gctgatggcc gataataaag gaatgggaat tggtaatatc
     3301 cgctcgagaa tcagctcact tggcggtaag ttcgaaattg aaagttggcc cgatgaaggt
     3361 atcattgtta cagtagagtt gaaaattcaa gatgaaaata aaataccaat ttagcgccgt
     3421 tataaagctc gtaagaaaat agattaaatt agccaaacag ttattcgttt atttttctgt
     3481 gttcggatgc caatggatta agaaacaata atttactcta aaacataatg tttaaatgaa
     3541 ctcttttagt taaaaatgcc aagtttaaaa tgaaaagtag gaaatttaca ctctcaatag
     3601 ttgatgatca taagttattt cgcaacgggc ttcgcctttt actcgtcaat agttatccga
     3661 atattgagat taccgaagcc aacgatggca atgaatacct gcaatcactt gcaactcacc
     3721 ttcccgatgt aacgttgatg gatataagca tgccaataat tgatggtatt gaagccacac
     3781 gccgtgccac cgagttatat cccgatatca gaattatagc cctgtcaatg tttggcgacg
     3841 aagcctatta ttacaaaatg attgatgccg gtgtgaaggg gtttttgttg aaaaactcgg
     3901 aaatagctga agtgcgagaa gctattgagt cggttatggc cggcgatcat tacttttcga
     3961 acgaattgct cctaagtatc gtcaaaaatt tgaataaaaa aagcttggaa gaagacacag
     4021 gaattaatct gtcagaaagg gaaaccgaaa ttcttcaatt gatttgcctt ggcctttcaa
     4081 ataaagagat cagtgaaaaa ctatttttaa gtaaaagaac tgttgataaa catcgcgcca
     4141 atattctttt caaaaccaat tcgcgcaata ctgctcattt agtaatgaat acaataaaac
     4201 ataaatggat tgtattttag caaacgtttt taattgattg tatcggaagc ttttgcaaat
     4261 acctttctga tttaatatgt tgaacgtcaa atttatttag aaataattga gcaaaaaaaa
     4321 taattctgat atctcaatct tctttcagaa agtgtagagt ttgatcttca atagccttta
     4381 atcttaattt agccaaggtg caaatgaaat attaattttg ccgcctattt atcatattat
     4441 tttataactg atttaataaa aaaacatcat gaaaaataaa cgtataatag ttattggagg
     4501 ctctgctgct ggtcctaaag cagcttccag ggctcgccgg ctcgatgaaa acgccgagat
     4561 cattatgatt caaaaggcat ccgatatgtc aatggcatct tgtggttatc cctattatgt
     4621 aggaggcttt tttgatgatc gcgatcaatt actttgttca cctgccgggg ttatccgaga
     4681 ttcgaaattt ttctggaatg ccaagaaaat cgttactcat gttaatacgg aggttacatc
     4741 aataaatcgt aaagcaaaaa aagttgaata cgaaaatctt cttacaggag aaaagggcac
     4801 gttagagtac gataagctta ttatagctac cggggctgtt ccacgcaaac ctcaaatccc
     4861 tggcgccgat cttgacggaa taaccacttt gcaatcatta tccgatgccg attatttgcg
     4921 aaaagtaggc gaggagggta agataaaaaa ggccgttgtt attggtggag gactgattgg
     4981 gatagaaaca atggaagcgt tgaatctggc ggggattgaa ataacattaa ttgagctttt
     5041 gccacagctt ttaactttcc ttgacaaaca tttagcccgc ctgattgaga aatacataca
     5101 aaccaaggca aatgtcattt taagaaatgg cgtcgcttca tttttgggag aaaacgggaa
     5161 gctaaaagct gtaaaactaa atgatggcac tgagattccg tgcgatttag ctgtagttgc
     5221 aataggagta attccaaata gcaaacttgc taaagatgcc ggcttggaaa taagcccaac
     5281 cggcggaatt gttgtaaatg gttatatgca gacctccgat ccggatattt acgctgtcgg
     5341 cgattgcgta gagataccaa acttgataac acaaaaaccc gttcatgctc cctttggcga
     5401 tttggccaac ctacaaggac gggttgcggg cgagaacgtg atcttgggaa atgtggccaa
     5461 atatcccgga accatacaaa caggtgtttg taaggtgttt gactatggtg tcggatcaac
     5521 tggcttatct gaacaaaatg ccattaaatt aggatttaat aacatcgaaa cagttgtaaa
     5581 caccagcctt gataaacccg gattcatgaa aggacaatta ctgataacca aattggtagt
     5641 tgataaatca agcggattaa tactaggagc acaagtaatt ggccctggcg atgtaagcaa
     5701 gcagttggct atctgggcaa tagcaattaa gggtaaattc actgttgaag atatggtaaa
     5761 tgccgacctg ccttacgctc cgccattttc cctcgccatt gatcatagca tcaccacggc
     5821 tcatattatg caaaacaaac tgagaggatt gttcaaaggc atatcttctg aagaactgaa
     5881 aaataaattg aacaacaaag agccaatcca tctgatagat gtacgtaatc cgaacgagtt
     5941 cgaacaaatg cgccttggca ttggcgaaaa acttattccg ctcggacagc tacgtagtcg
     6001 tattaatgag ctgccggaag ataagaacac cgaaatcgtt gtttggtgta aaatatcgct
     6061 taggggctac gaagcatcat tggtgataaa agccaacggc tacaaaaatg taaaagtcct
     6121 cgaagggggt atcatggcat ggccatggag ccgagagaag taacaatagc tcattcaaca
     6181 aatttactgg gcgagggtaa atcttagcgt tatacctgct ttagtagttg aagtgcgata
     6241 ttgtgaagat acaaatggat tgttggtaac tttatcgaag aagattcgga ggttaagtcg
     6301 ttgatttacg gtataatcgg ctgaaaggtt gagtgaggta accttttgac ctgccgacac
     6361 ttgattaaga tcttcatcta tccttcgaag cactgtctta tcggttttta aggagaaatc
     6421 ggctctaaca ttcaaatcgc tttttacagt ttttctgctt ccctggctgc cgccggtagt
     6481 gcttcttata ctaaactgca cctctttaaa acgatatccc aaccctatta catactcgtt
     6541 tgagttaatc tcggtaagtt gattgttgac aaatcctaac gataggcttc ttgtcttttt
     6601 aatttcaaac ttggacatca aactgttaat ccagttcata tcgaatgata tgagtggcat
     6661 aaactgctct gttatgctca cctgcgctat taaatgttca tgaataaaat tatgattgtc
     6721 atcgaacgct gaagcgcccc cattaaactc acggtagagg aggtttgttt gaaatgagcc
     6781 aacgctgtaa ttgctccgat aaccatgtgt tatggttaac gttcggagga attttttgaa
     6841 tggttcgagc cttgcaagtc cattatacgt taaacgccaa ttcagcattg gtatggcagg
     6901 cattgtgttt agtttgatat tagcggcctc tttgcctgta taagctgcca aaaagctata
     6961 tagaagcacc tcttgttgcg atgagctata tccttcgggg aatccggtag aatcaacttt
     7021 tgtttttgac cagttgggat tcgcgttagc cagcctaaaa gctatctctt tgcggttttc
     7081 ttttaatctc tcaaaatttt cattttgatc tttgtcgtta tcctttgccc atgccgtacc
     7141 ccatgtaagt accgatacac tgaaattacc ctgttcctgt ggcgaatagg aagcaaatgt
     7201 tccattggca tctgctttga aatattcctg atggttgagc gagtagtttc gatcggcatt
     7261 gagctcaatt ttaaaatcag caaatggttc aaaattacct ctaaagttta tctgttcatt
     7321 aaatttcgtg atatatgctg aatttaacaa agtatccatc gtaagccagt tgtttgctac
     7381 cgctttgggg cgtatatctt tttggctgcc aaaaataaat cccattcctg gtgcattttg
     7441 cccccagtcg ttgcctacat aacccgcttc gggtacaaag ccgggtaata aaatgccatt
     7501 accttgtgaa taagccagcg aagcacgttt aaagccggtt aatagtctaa gaaaagtgtt
     7561 tcccgctatt ctgaaataat caggtctctc ttttttaacg gtatcctgtt ccgatttttt
     7621 ttgttgctgt tgttgcattc ccgggcgtgt catcccacct gcccgctgcg ggcttcgtgg
     7681 ctgatttatc ttttttagtc cgggtatctt atcataaatt ctatcaaagg ataagcttcc
     7741 atttaattga atgttgctag agttttcaat ctgattaccg aaacgtgctt gtagcgactt
     7801 aggcgaagcc tgccagcgat aattcacctg atagcgcgcg ttggcagtta cccaatcaag
     7861 taatggcagc ttgctgatgg gaagggtata gttgatccct gtcatctgat tatataatgt
     7921 cttgctaccc cagtttttaa tctccgtttt aatcctctcc ttttttattt tgtaatcagg
     7981 atcatccttt tcaatgcttc cgggcagctc gtcaatatat gagcccacat cggctttata
     8041 gtctattctg agcgaacttg ttaaatccca gttcaagctg taattacgtg tccagtccca
     8101 tttttttaaa taagtaggct ctatcaatac cgcatattga ctcttattac gcaaaagacg
     8161 tttgttgtat tcgcgtatca tatcggtacg gaaggagaag agcttaggga gtaggtaaaa
     8221 attaaagtct tttatgaaag tcaacggttt agggctgaaa aatttaatag aggaaaaagg
     8281 acgatagttt tttgggacgg tattgaagtt gtaaccgatt cctcccagat atttcttctg
     8341 tatatcgtat tgataatcaa cattacgggc atatatttcg gaatatgaat aggtgaatcc
     8401 ccagttttcg atatcataaa aattggtttt ctgtgaggcg cccatttttg ttttcctcat
     8461 attaataaag tttatgttct tgcgctgaac ataatcctga ttgattctga ttattgaatc
     8521 tctatcttgt ttcgtatcga atgttttgac atcctctctg agtaaaacat ccggatcgag
     8581 caggttatat ttagggtctg tcctcgattc cgaatagtcg aagtgcattg gtatcttaat
     8641 accggcattt tcagggaaaa acttacctaa ttcgaggttg gaggagaagt cccaattctt
     8701 tataaactct tttgatctct cgctggtttt cttttcaatt gttccaaacc cgggtgtgct
     8761 gaataatcct gtaagggtga tatttccaaa gtcggagagg tcgcttctaa gccttgctgt
     8821 tgcagcaaat cctccttggt tattgaagtc ggtaaggcgc aattcgttaa cccaaatttc
     8881 cgcacattta ggctctccgt catcatttat cgaaaatgag gtttgtttag gatttctaac
     8941 cccaatcatt atggctttta cactactcag atttgggctg ccgagtattg ttaccctgtt
     9001 attaccatcg aaagcataaa aaggcattgt caggctgata ccggtggctc cggcatttcg
     9061 catctgctca ttacgttcga ttttaaactg aacaagacgt ttcaagtcaa ttttaaactt
     9121 attctcttca ggccatatca gatcaggctc attatatgat gtatgccaag gtgtgaaatg
     9181 taatggtatc tcatattcat aataattttc ggtgaggtcg gcgccaaaac gcataaagac
     9241 ggtgaggtcg ccatggttaa gctcctgatt gtctataagt ttttcggcat gtacaaacat
     9301 ttcgataaag ccataatctc tgaaatcgaa gtcggtagtt ttataagccc ctctcgcatc
     9361 gccgtcaatg aggttacata ccctcaacac cattgattgc tcattgagtt gttgcaggtt
     9421 tgtggtgcct aagttcactt cacgttcaat tcccggcggg atggcgtaag ggataggctg
     9481 ccttttgccg ttttcttcaa tgttaacgga aagaatatcg aatgaggtct cgctctgaat
     9541 atcatttggt atgtactcgc caggagctaa cagatcatat cggtatcttc tccactctcc
     9601 tctaacaaga tcaagggtag caaaacgtag gacgatcggg ttttcgaacc ctttcaaaaa
     9661 catcctcaaa aagcgtattg actgaaaatc ctgaatccct ccgatgatct tctcagggtc
     9721 tcttaccgga attttgaact gataccattt tacctcgccg atctgaccat ttggaagtcg
     9781 gataccttgc gcattgtata tgtcagtaat ataattttca ccaacattca tcttgttggg
     9841 gtcaataagt accttgtact gaaaatagcg ttcatcatcg ctaagtgtgt tgtcgcggtt
     9901 tatatcttca gcatcaggcc gagtagtagc agcggtagga tatgattcgg ggttaatgtc
     9961 ctctgtgggc gagttcccat cagggccgtt aaagttttta taacgttcca ggatactgga
    10021 gtatttctcg tctttatcat aatcagaacc tctgaaatag tgataattat cagaagaagg
    10081 gtctaatata gcctgtttgt aagcttccga ttcggaacca tacaaagctg caagaggttc
    10141 gagaaattgc gtttgaaaaa atgaccgttc atcttcagta cgtagtccat ccaatcccac
    10201 atcctggaaa gcccttgatc caggtacatt gctgaagtta tccactaaag cctgaaatga
    10261 aggcaccctt ccccatatcg ttgtatctac attctctacc tcttctgttg gcggaagtcc
    10321 atgctcaaaa ctttttcttc catcgcgtaa gatatcttcg gaaatatcac caagattaaa
    10381 ataaagctcg ccggagttat cggcgccttt tacaaaggga tccatcaacc agaactcaat
    10441 gtattctacg tttgtggcat cgaagtttgt tgactcgatc ttacgcatga tgcctcccca
    10501 acgtgtttct ggtaaattta gtaacccgtc ggcattgaca cctgcagata ttcctggctc
    10561 cccttcaacg tcatagttat aaggtcctcg ctccgaagga taataggcca gattaagaat
    10621 tggaatgtta gtaggtacgc cgtttgggac atctttattt gggaaaacct cagtctcgag
    10681 cacctgccta acctgatgat ccgaaagttc gttaaggttg atatttgaag gtctatggct
    10741 accggtctcg tcgtaaaaaa gtgggtctat aacataaaag gcaagttttg cacgattaaa
    10801 accgtatgcc agtccagtgg agggcgccgc ttctggaaac atatcggctg ttgtctgttt
    10861 ttgaggtgta ctggagagtt gccaataggc catatttttc agatcaatgg tagatttagc
    10921 agcctcaaaa tcgtcaatgt aggaggttcc tgtggatccg ataactttag catgaccagg
    10981 gacaaaatgt gcaaattcac catctatgtt tattctggat tcatctttgg ctgcaataaa
    11041 aggcagttta tccacaattt ttgttatcag cctcgattgt gtctgatagg cgacatccat
    11101 accccagatt gtatttgaaa tagggtcgtt gccgtagctt accttttggg ttaacggtct
    11161 ttcggtaagg ttgagaaggg tgcctccgat attaaaattt tcgtttatcc gataatcaat
    11221 atgtgttccc atcatccttt tagtctgtac actaaaaaat gactggcttt cgagcgaaat
    11281 atttattgga gttcccgaat tgagcacacc ttcattgatg atcctcacac gtcctaatgt
    11341 atagtccacc gtataatcta cattttccat caaaggggct cctcccgctg ttacttttac
    11401 cgaaccttgc ggcacgttga tagcgttcaa cgagatttcc gatcctgatg aggatttata
    11461 aaatccttcc aacatgaatt tgtttttctc aggatactgc tgagctcccg tttttgtcaa
    11521 tgtgtatagc gaatcataaa caaaacgatc ggcaatatca tcatctccta ttttagcgcg
    11581 taaatagctt ccaaatggct ctaatacagt aaaatagact ttaccgtttg tagattgaat
    11641 ggtgccgcct tgtgtggctg cnnnnnnnnn nttatcaata aagtcgaaga taccatcata
    11701 gggtggattt aactgagggt caaggttgtc gaagttgagc accctgataa gcgggatacc
    11761 acttgcattg ccggcgtcgg tcagatagct tgtcggtaca gcattttgat ttcctgaata
    11821 gaggatattt aaaatgaaat ccttgctttg aatttgataa gctccgatgg ggtaaacgtt
    11881 tttcatcatc agattccaaa taggaaccct tgtgctaagg gagttgcttt tcaacaactt
    11941 gacgataagg tttttcggtg agtttatacc gccgtcagaa aattcgccta cctgataaag
    12001 tgtcgtgtcg ccaataacat tgtactgaaa ggccacagca agaacctgat caggatttag
    12061 ggtggtattt agcgagataa agccaagttt gccgttaaaa gtgtattcgt ttgggctgag
    12121 ctttctggcg ctttccacct tcactgcatc aaccccggct tcaaaataaa atggcgcgtc
    12181 tgtgattatc tgcgatacct tgttaatatc cctgatatcc ggctcaaaag ataaaagcat
    12241 ctccatcagg ttattggaac gatagttatc aggaaatacg cttggctttc cggtaacatt
    12301 cgggctgtat gggttttttt ctcccagatc ctgtaatgct atgatgttac gattgtcggt
    12361 aacggcagcg ccaatgtttg ttacccacac ctctatcttt gtaatgttaa cgtttgatcg
    12421 tatcacaggt agttcggaga gagcagtatc atacgtctct ctaaaatatt gagcaagtag
    12481 gtaatgttta ttttcttcgt attgatccga attgagtaaa aaagcattag tctgcgcgcc
    12541 accttgaact gttatacttt gagtctcact cttttgttcc gaatacacgg tggttacagt
    12601 tgcacgtcca aacttcagtt tggttttaat accaaaaagg gcctggcttc cagtgattag
    12661 cgtgctgtta agaggaagcg taacatttcc ggcttcaatg agttggataa tctcatcctc
    12721 ttttccttca tattttaggt ttaatttatt ctcaaattca aatgaagcct cagtattata
    12781 gtttgtttga aactctatct tatcaccaat cttggccaat acgctcatct gaatacgttg
    12841 ctggaaatca aaatttacta tgttctgttg ccttacactc aatgtagggt cttcgcgttt
    12901 gttggccaat acgccgaagg taagttcagc cgagccttgt gggcggatat ctatcgttcc
    12961 tgatccaaag atcttctcaa aaatttcgcc gccgatataa atctgtggta tcaggccggt
    13021 gccggaggct ccttgaccgg cagaaattgc acgttctcgc caatattgtt gtaaagccct
    13081 ctcttgatcc cacttcatgt aatccgtagc tgtcatagaa tagggagtac ggtaattgag
    13141 cttacccatt ttttccttga tcgtgtactc atttgtttca gggtcgtact caacttgatg
    13201 ttgaacgttg gaaggctctt taaggaaaag aggagattca ggttttgatt cagttactga
    13261 tgttggattt gcaaaaggat agattaattc tatcgtatca gggcgtaaaa accgaaaagg
    13321 atcagggttt aaaacatgct tattggtctc tttaacagtt gaccgattga ttgcggccaa
    13381 cgaaaagaga gccagcaatg cgaaaagtag tatatatgtt aatctacgta tcaaaaagta
    13441 ttatcagttg ctagttgata agcaatcaaa aaaaatcagt aagctttgat taaaggtaac
    13501 gcagcgaaag tcatttaatt aattggtttt tagctaaata aaagataacc ttctcaattt
    13561 cttactattt acaggatttt taaagcctcc ttaattagtt cttcaacgct tagattcgga
    13621 ttctttttca gaatagaatt taatgctttt tctgatgcat gttttccaaa acccagcgta
    13681 gttagcccaa ttaacgcctc ttctttttgt gtattgtgcg tccagcctaa aaattcaggc
    13741 gcgagagcct cttttcctat cttatcttga agctctacaa tgatccgttg tgcagtcttg
    13801 ttgccaatcc cttttatccc tttcaatgtt gcaagttcgc ctgccctgat ggtttctgta
    13861 attttttgat acgacagcga agatagcatc cgcatggcag tgctgctacc tactcccgat
    13921 actgaaatta acatacggaa caacgatcgt tcagcttgat caaagaatcc ataaacaaca
    13981 ataccggtgg gtgttgtggc ttcgatctta aatgtcagat gaacgtatag tttcgcctca
    14041 tccaagtctt taattgacga aaaagtattg agtgaaatat taattagata acccgtacca
    14101 ttctgctcaa gaatgacgta agcaggattt ttttcaacaa gtttacctga aaaatactcg
    14161 atcatagccc tttattttaa tctgatttag acaaaaatat attttttcca ttccaaatga
    14221 atgtttagcc aatccattac tgttacatga ttgctatccg aacaataaag gtttaagata
    14281 aaacaacaat attgtttttt tatttcagta tctcaaggat agatagacga ctttaaagag
    14341 attaccgaaa aactatattt ttgtcaggaa taacctatta attttattat tttgaaaaag
    14401 agttatttac tgttgtttta tagcattttt gcggttttta tacacttttc ggcagttgca
    14461 caaattagtg aacagggtac tccacccagt atagaatatt ctggttcatt atctcatcat
    14521 cctgttgtaa ctgtgcctcc gcctgatata gagcttctaa aaagtgagga tggattttat
    14581 cagaagaatg ctattgctta ccgttttgct gaatcaatac ctgttaaaat agattttgcc
    14641 aaggaggggc tgtgggaatc attgcctgat ggtagaagga tttgcagatt aagtattaaa
    14701 tctcctggcg ctcaggctct aatattatat tacagccgct ttaaaattcc agccggtgga
    14761 aatcttttct tatacagcgc cgacaaaaag cagattattg gcgccttttc ttctaataca
    14821 aactctgccg acaaatcctt tgcaacggaa atgatcaaag gtgaggaggt tattctcgag
    14881 tattcagagc catttgcact atcagttaag cctcaaatag tgatcaatga ggtaggttac
    14941 gtttatcgca ctgccgatcg tttttttaat atgcgtgggt ttggaacttc cgacacctgc
    15001 gaagtaaatg taaactgccc tgaaggagag gagtggcaat accagaagaa cggcgtggtt
    15061 aggattatcg tcaaatctgg cttctcatca ttttggtgtt cagggtcaat tgtgaacaat
    15121 gcacgaaacg attgtacacc tttcctgctt accgccgatc attgtgggcc acatgcctcg
    15181 gaagaaaact atgaccaatg ggttttctat tttcgttacg aaggcgatga ttgcgatgac
    15241 ccggagagcg attccacatt taaaagttat acaatagtag gagctaaaaa gttagcaggt
    15301 aacggcggtg cagcaggtaa agcatcagat tttaagctgc tgaagctcaa cgaaaacatt
    15361 cctgtaactt acaatccttt ttacaatgga tggagcattg ccaatgaacc aagcccaagt
    15421 ggtacttcca tacatcatcc cgatggcgat attaagaaaa tcagcaccta taccgacaca
    15481 ctcaagtcat caaactgggg gacaattccc aatacccact ggcgggttat ttgggcgcaa
    15541 accgaaacaa actggggcgt tactgaaggc ggatcatcgg gaagccccct gttcgacaac
    15601 aatggaagga taataggaca gctcaccggc ggcgaagcaa gctgtaaata tgtatataaa
    15661 ccggattatt acgggaaatt ctcctactca tgggatggag tgccaacagc agactccacc
    15721 atgctcaagc catggcttga ccctgatgat accggaataa ctgaaataga cggaatagta
    15781 agcataaacg aaccaattaa gcgatcgtcg gccttgtatc tatatccaaa ccccgcagaa
    15841 gaggccattt acattcggga tgagcggata accggaaacc ccgttcaggt tattatcact
    15901 aacctgcatg ggcaggaggt tttaatagaa cattttcctg agcgtgccga taatatgatt
    15961 aatctaaatg ttaacaccct caaaaaaggg atatacattg tttacatcat tattaatgat
    16021 gaattaagaa cagcaaaact gataaggtaa tcggataaag agaaatcgca gttaagaaca
    16081 agcctttaaa aggctaacaa aacggacatg cctccgttac aacactctgt tgttgttaag
    16141 tatttgactt tgtgtcttca acaataatat aaatctcagc gctctttaag cgctatttgt
    16201 tataacacgg cgttccgcta tctaaaactt actatttttt aatgtgcttt gccagaaact
    16261 tttccatcgc tctatagaaa tcgaaacgat tctcttcatt tgcaaagccg tgtccttcat
    16321 tatcttttac catatattcc acctcaatgc ctcttttgtt taatgcttcc acaatctgat
    16381 ccgattcggc cttgttgacg cgcgggtcgt tagcgccctg tgcaacaaag agtggagtta
    16441 ttattttgtg tgcatttaaa gcgggcgagg tagctctaaa ttgtaccgtg tcaatttcgg
    16501 gatttccaac catttcgtac atcatgtcga gcaatggctg ccaatatgga ggtatggttt
    16561 tcagaaatgt gaaaagattg gatacgccaa cataattcac cgctgcagca taaagagtgg
    16621 gggttaccgt aacgccttgc agtgtggcgt aaccgccgta gctaccgcca tagatagcta
    16681 tccggtcttt atcggcaatg cctttgttga tcagccagta aaccccgtcg gttatatcgt
    16741 tttgcatagt gagaccccat tgtttgaatg acctttccca aaagtccctg ccatagcctg
    16801 ttgatcctct gaagttcatc tgtaagacgg cataaccgcg gttagcaaga aactgaactt
    16861 caggattaaa accccagcta tcgcgtgccc atgggccgcc gtgcgggttg ataactacag
    16921 gtaaattatg agccgtttcc attgttaacc cattaggtag ggtaagataa cccggtataa
    16981 ccaatccatc gcgggcttcg tattcaatag gaaacacctg cgccatctta ctttcatcta
    17041 accaagggct tatatcatgg atctttgtga gtttgttttt cgcaaaatcg taaaggtaat
    17101 aagcgccgag tgaacgatca gaatacgaac ggatgatata catattttca ttgtcgtcca
    17161 aagaagagat gccgatttcg tagttaggaa gctgtttttg aagattctca aataacgttt
    17221 tagtctgctg gtcgaagaaa tgacgctcac gcttccacga aacaaaagag gcagaggtta
    17281 ccactttcct ttttctggaa aatgaaaccg atgaaatatc ataatccgga tgctcgtaaa
    17341 gcgcctctat ctctttacca ttggcaatat caaagagcac gacaaccgat ttatcccttc
    17401 caagattgga agtagcatag acatttttat tgtcgaaatc aaaaaaatat ggcgacaccg
    17461 attccttaaa gttggtagtg agcacaggtc taaactcctg atcttcactg tcgcgataaa
    17521 gaatttgtgt gtttacacca tcaacaatag ctattgcaat acgtaacttc ccttcatgat
    17581 ctgtcatcca cccctggata tttccaggat tttcggcaag catggtcatc tcaccggttt
    17641 tgatattcaa ccggtatgga tcaaatacca ccggagtacg tttgttcaaa ctgatgatca
    17701 cttcgtcagg gaaatccttt aagtcgtcaa cgatcattgt tcgaacaccc tcaaagcagg
    17761 taagacattt taagtttttt ccatccctgt caacgccgta aagcgcgaaa ttctcatcac
    17821 cccctttatc ttttaagtaa agaatcctat tcttgtttgc ccagtagtaa ccggcaatat
    17881 cccgatcggt ctcgttagtc aagcgaatag acttctcttt gccaatctgt tgaacaaaaa
    17941 tattaagacg gttttcataa ggtgccaggt aagaataaaa ctttccatca ggcgagattt
    18001 gataaccggt cttttcaggg tttcggaaaa aatcctcaag aggaatttga ttttgtgcca
    18061 ttacactttg ggttgaaaga agataaaaca aagaaactaa aaaaaatgtt cctaaaaagg
    18121 agcaacagtc tgactttttc ataaataatg atttttgatt aagtgtgcaa atataggttg
    18181 ttctaagttt tcactaaaag gatttctcct tattaatttt cgcttcattg gatcaaaacc
    18241 attttttttg ccgcatatac agatacatgg ctgtgccgcc ggcaactata aatatcagta
    18301 ccgcaggata actccatcgt aaagaaagtt cgggcatata ggtaaaattc atcccataga
    18361 taccggtgat cagcgttagc gggatgaaaa tagttgcaat gatggtaagc gacttcatta
    18421 cgttattcat ccggtttgag tagttcatca tctgaagctc atacaggctg gtaatggttt
    18481 cacgataatt gtcggtggcg cttaacaaat gctgcaaatg gtcagcgata tcgttaaaaa
    18541 aagtgcgatt atgctttttg ataaaagcgc tttcgatttt taataaaatc cttacagcct
    18601 cagcaacagg caatatatta cgtctgataa aattcagctc tcttttctgc tgttgtatca
    18661 tacttgtcat atccgcactt tgatcactca tcaggatttc ctctatctct tctaactttt
    18721 cgtcggtttg attaaaaagc agataataat gatcaacaat gatatcaata agctgataca
    18781 atatataatc gtgggtataa tgatgagggc tctcctttac tgttttaatt cttttgataa
    18841 aattatcgaa aagcgaagtg ttgcgctgga ggaaagtaat tataaatctc ttaccaagca
    18901 gaaaacagac gtgattagtc tcaaaagagt ctgtctcagt atttcttgtt aatattttta
    18961 tcacaaaaaa caaaaactca tctgtctctt ctgcctttgg caaatgctcg gcatttaaaa
    19021 tatcttcgat gatcaaagga tttatttgaa atacactgcc aagatgttcg agtttatcta
    19081 tatctcctaa tccagtgaaa ataagccagt ttgttgcttc tggccagagc cgtgaaggga
    19141 gcttctcaaa atcctctatt atgatgtcct gattgctatc atcgcttacc gaaattagcc
    19201 ttgtggattc gtgtacagag gtctgtgcaa atgtattgat cagcgtgccg ggaggcgctc
    19261 cggccttcgt tttcatcaac ttgaaaatct tgctctgagg tattttcata gtaaagttct
    19321 ttttatttaa tgcacaaagg tacataatca actaagaaat ctaaaataat aaggtattgg
    19381 ttttaacgtt tgattccata tctgttacaa tggtaaagtt caacatttac gcgcgcaaaa
    19441 aacaatggaa attgttcctc tttgctcttg cggttgcaat agtaagcgga tcaatgtact
    19501 ataccagtat tattgtacgc aaagtggcgg agcaggagca gaccaatgta aagatctggg
    19561 ctgacgctat ctaccgtaaa accgaacttg taaagtatat cgaacagttt ttccagcaaa
    19621 tacgcgaaga agaccgtaag agtgttgtac tgttagctga agcatacaaa caactgatcc
    19681 gtaccgacga tcctaatgtt gacctcacct tttacgtgga tataatacgg tacaacagca
    19741 caatcccaat aatcctcacc gatgaagacg ggcggataat aaacaccaaa aatgttgatt
    19801 ttaacgaaga taccgttacc tttttgtcag gtaagctctt agatgaattt tccgtttatc
    19861 ctccaattcc tgcccgctat tttcaaaata aaaagaacta tctctattat aaggattcaa
    19921 agctgcatag cgagctgcat caagtgctca acgaccttgt cagtgatttc ttttccgaag
    19981 tggttatcaa tgccgccagt gtgccggtga tcatcactga tagcacaaaa cagaaagtga
    20041 ttgattttgg aaatatagag ccaaacaaaa tcatggagcc gctctttgtt gaaaagacca
    20101 ttgcagagat ggcagccagc aatcctccta tagaaatcga acttaccaac ttaggcaaaa
    20161 gttacatttt ttataaaggc tcatctcttc ttacacaact gcgctatttc cctatagtcc
    20221 aaatcctgat tatgggtctg ttcctattta tcggatacat gatgttcaat actgcaaggc
    20281 gatcggaaca aaaccaggta tgggtagggt tggctaaaga gacggctcac cagcttggaa
    20341 cgccgctttc gtcaatgatg gcatggatgg agcttttaaa agaggaaaac ccaaatccgg
    20401 agattgtaag cgagctaagg aaagatgttg aacggctcga aaaagttacc gaccggttct
    20461 ctaagatagg agcgctgcca aaacttactg acacaaacat agtgaaggtt atacacaact
    20521 caatcgaata tctgaagaca cgtacctcac gtaagatcac atacgagatc acccccggcc
    20581 ccgatactga aatcattgct ccaattaacc tgcacctttt tgaatgggtg atcgaaaacc
    20641 tgacaaaaaa tgccgtagat gcaatggaat caaagggtac cttctcagta aatatcgaag
    20701 agaataatgg tcatatcatt attgacctta ccgataccgg gaaagggatc ccaaaatcga
    20761 agtttaaaag catttttaac cccgggtata gcggtaaaaa acgtggctgg gggcttggcc
    20821 tctcgctggc gcaacgcatt attcgcgact atcaccgcgg caaaatattt gtaaaatcat
    20881 ccacaattga caaaggcact actttcagga ttatcttgcg caaaaaagta aagctgtaac
    20941 ataatatgcc agggctttat atccacatcc cgttttgcag gcaaaaatgc cactactgta
    21001 atttcttttc gatagcatca aaaaaatata ttaacgagat agttcaggca attcaaactg
    21061 aggtaagatc aaattccggg ttttttaacg gtagtccatt aaataccatc tatttcggcg
    21121 gaggaacgcc ctcgctgctt agcataaacc agattaacga gataatagat acggcgatcg
    21181 ctacatttgg aatagatccc aacgctgaaa taaccgtaga ggcaaaccct gacgacctta
    21241 taaatagttg gctccaagcg ctctcgcaaa ctgttgtaaa cagattgagc atcggtgttc
    21301 agtcgtttag aaacgaggat ttgacttacc tcaaccgtgt tcattcagca aatcaagccg
    21361 aaagtgcgat tcaaaatgcg ctgaagtatg gttttaaaaa tatcagtatt gacctcatct
    21421 atgcaatgcc aacactcgac aacgagcaat ggattgaaaa tatcaatcgt gcgctgagct
    21481 ataatatccc tcatatttcg gcctatgcgc ttaccgttga gcctgggaca gcgcttgacc
    21541 ttttcataaa aaaagggaaa tataagcagg tggaagagga gaaagtggca gagcaatttc
    21601 aaattatcat gacaagtatg gcaaaatcag ggtatgagca ttacgaaatt tccaattttg
    21661 ctttaccgga tagatattca cgtcataata aaagttactg gtcgcgcgaa agttatctcg
    21721 gctgcgggcc gtcggctcat tcgtacaacg gttttataag gagatggaat gtttcaaata
    21781 tttctaacta cttgaaagct gttagggaag gcacgacata ttacgaactt gaagaactga
    21841 caaaaaacca acaatttaat gaatacatta tgactgcctt acgcactatg tggggtgttg
    21901 ataccgaatt tgttaaaaaa ctctttggta acgactttgc aacccatcta aataatcagc
    21961 ttaaaaaacc attggaactt gggctgatcc tccaaaaaaa tcaaaaattt attctctccg
    22021 atcaaggtaa attatttgct gacggaatag cagccgaact ctttatctcc tgaaagcgta
    22081 actgcattga agttagcctt ataaaatcaa catctcaaca taatagggct ttcttaacag
    22141 caaattggat attccccttt ttgcttatct ctttgttgct tgttatatta ttgcgtgtta
    22201 gcaaaagtgg ttattaaaac tgcccaaatc ctattactaa tgagcataga aaatatggag
    22261 tgtttccggt agaatttaaa tctttaaact ttaatatttc attgtatccc gtttcaaatt
    22321 tgatgctaat atgcccattt gccgtacctc cataataata gtagttttta ctttcaaatt
    22381 cataaatctt aacttcggta tgtaagccaa ttccgtaagt aaatgaattg aaaatctcat
    22441 attctaaatc cttatccttt gggtcgtcat attttgtgct ttccagatga ctgcctgata
    22501 tagaagcata aggagctaaa tgaaaacgat tgctcctaac aatgaaatat ccgcctttta
    22561 aacctgcatc taaatatgaa aatttttcat ttaactcaaa atttaatgta tcagtatctg
    22621 aagttaccat aaaggggtct ttcaatttta aacttgttcc ttttaagtaa agtgaggcaa
    22681 aaactctatg aacattaata tccatcccaa aattgaatag ggcattattt gaaaatttag
    22741 tggctaaatc acctgttgta aaaaccatac ccgaaccaaa aaataaattc caggaggctt
    22801 taattatcat gtgcggcata aatccattta caaagctgtc atatttctta ttgtcatact
    22861 tctttttata tgcaacaagt atttcattgt attctttatc tggtggttgc ttttcaatat
    22921 attttaaaaa accggtaata agcagctttg cttgttcatc aatctgcgac atgttgcaac
    22981 ttaccaaaat agattgtctg ttactcaagg tcagttcgga taatttataa actattgtat
    23041 gggaatttgg ataaacaatt ctctttttgt gtttttcata ttcaagcatc aggttattta
    23101 ccgtttccca atcgcctaca agcatattaa tataaaaatc ctcaataaaa tagaatgcag
    23161 aaaagggctt atcttttgtt ttctccgtta aaaaatcata gatttctttt gttttaacca
    23221 aattgttatc cgctagctct ttaagaatca tcttcctgcc attatgaact aagagttcag
    23281 tactatcaac atatgttgct atctcattac taatctgaga aaagatactt aatggaaaca
    23341 taatagcgat caaaataata gttagatttc ttttcatgat ctgtttttat taatgttcaa
    23401 ctctattgct gttttttata ttaaaagtct gtaatgctat tttcgttaat aggtttataa
    23461 agcgccttcg ttttaaaagt acggttaacg ctccggttct ggttggataa ctgcaccttt
    23521 ttgctcaaga ggctggtaag tttatctgtt tcaatggtca tcaaagggcg ccctgatttg
    23581 ataaattctt tttttgtggt acatgagcat atttttttgt tgttttcgta cttttatgaa
    23641 ctaataactc ttgaataccg caaaccagtt ccgcttacaa gcgaatggtt aacgtaataa
    23701 tgtcttaacc attaaagcgt taccgatttt ataattccgt acaaacataa cgcttttttt
    23761 aaaactttcg caagcggttt ttcagaatat ttttactctt cttgctgccc tttaaaaagt
    23821 cattagagcg tgggttgatc ctttaaaaaa atcaaaaatt tattctctcc gatcaaggta
    23881 attatgtgcg tttttctgct ttgaacctaa cagccaagta tataccaaat tgcggggttt
    23941 taaggcatca ttgtttcaag tttgcaaaaa ggtttattac ctgcagaacg tacaatttgc
    24001 cacaaaaccc cgcaattatc acttagacaa tgttatgggc tggtttttat tttccgccga
    24061 atttataacc gactgataat cctataaccc ggttattaat cttcattccg ccatcattat
    24121 aaggtgaaat atttgctaag cccaaattat agcccaatcc aatttgaaac aaatcaattt
    24181 caacaccagc gcctattgtt aaaccaaaat ctaatctctt taaatcagat ttaccttttt
    24241 cagagcccca ttcaacatct tctttctctt tttcagtttt gccatcaatt gttgttacag
    24301 ttttagattg accggttagt cccataccta tatacggacc aaacaaacca aatacttttg
    24361 cgtcacctaa atcaaacgaa gctttggctg ttaaaggaat atcaaggtaa aagaggttcg
    24421 ttttaatttt catctctatt tttttaccca taaatgtttc ttcttcactg atcttgtatc
    24481 cttttgttga taataacaag cctgtttcaa aagaaaacat atcactaaat ggaaattcag
    24541 ctgtggctcc aatatgaaat cccggattca ttttaaaatc atcgctgtaa gtggtgttgt
    24601 catctttggc aaggatattt gataagttca aaccgccttt taatccaaaa ttttgagcaa
    24661 aagcccctgt ggtcattgat acgactaaaa caaaaatcaa aagcttaatg acatttttca
    24721 taaaaaataa attttaattt atcctactcg taaggctttt cggattacgc cccgtttttt
    24781 aagtggtttc tggactccac ttttaaagtt caataataca ttttttttgt cactatgggt
    24841 ggccagcccg ttggctttcg cagaccatat ttgtttttaa cttgatcata actcttgaat
    24901 ataccttaaa tcggtaccgc ttacaagcaa ataggtaacg taaaaatacc ttactcattg
    24961 aagcaaaacc gatttagtaa ttcagcgcaa acataatcct tttttttaac ctttggcaag
    25021 aatttcttca gaatagtttt actctttttg ctactcttta aaaaatcatt agagtttggg
    25081 ttgatcctcc aaaaaaatca aaaatttatt ttcttcgatc aaggtaaatt atttgctgac
    25141 ggaatagcag ccgaactttt tatctcctga aagtgaaact gtattgagat taaccttata
    25201 aaaccaacag ttcaacataa aggggcttca tgtaaagcaa attgggaaaa aaaaagcggg
    25261 tttgatcaaa ttgtatttaa ctaagggagc attctttaca ctgtgctctc gagcattatt
    25321 tcgctgggtc tgaggttttt tattataaac ttctgacgtt cgagggtgtt tttgcccata
    25381 taaaactcca gcatctcttt tatggattgt ccttttcgca gcagtacagg gtcgaggcgc
    25441 atagatgctc cgataaagtg tttaaattca tcgggcgata tctctcctaa gcctttaaac
    25501 cttgttattt ccgggttggg tccaagtttt ttaattgctg ctttacgttc atcatcgtcg
    25561 tagcaatata ttgtctcttt cttgtttctt actctgaaaa gcggtgtttg cagtatgtag
    25621 aggtgccctg attttacaag atcgggataa aactgtagga aaaaggtaag cagcaagagc
    25681 cttatatgca tgccatctac atcggcatcc gtagcaatga caatgttgtt gtagcgtagg
    25741 ttttccaaat cttcgtcaat atttagcgcc gattgcagca agttgaattc ttcgttttgg
    25801 taaacgaact tcttgctcat tccgtagctg ttcaaaggtt ttcctttaag cgaaaagact
    25861 gcttgtgtgt ttacgtctct cgacttagta atgctcccgc tggctgagtc gccctcggta
    25921 ataaatagtg tagtttcaag ccgttgttca tgattttggt tatagtgaat acggcagtcg
    25981 cgtaattttt tattgtgaag acttactttt tttacactct cttttgccag ccgtttgata
    26041 cctgctatct ctttgcgttc cttttcggag cttaatattt tttggtaaat aatttcagca
    26101 gtttgtgggt ttatgtgtag tgtgttgtca agattacgct ttacaatgtc tatcacatag
    26161 ttacgcactg tctgaccgcc tggttcaata tattgtgatc cgagttttgt tttggtttgc
    26221 gattcgaaaa ccggttcttg tatttttatg ctaagggctg cagcaattga agttcgtaca
    26281 tctaccggct caaaatcttt tttaaagaac tcccttatcg tctttactat tgcttcgcga
    26341 aatgcggcta aatgggtacc cccttgggtg gtgttctgtc cgtttacaaa agagtgatac
    26401 tcttcgcccc actggttgga gatgtgggta aaggcaaatt caagatctcc ttcggtaatg
    26461 tgaataatag gatagagggc ctcgccgttg gtgtaacgct ctaaaaggtc gtgaaggccg
    26521 tttttagcaa aaaaacgttc gccattgtaa tagatagaga ggccattatt gaggaaaaca
    26581 taattccata gcagcttttc aacatactcg gggataaaat gaaaattgcc aaaaaagctg
    26641 atatcagggg tgaaagttac cagagttcca tttttctcat cagtagggct ttcagcataa
    26701 tcgtttataa tttctccttt actgaactct gctattttca acttcccgtc tcttaccgat
    26761 tgtactttaa aatatgacga aagcgcattt accaccttcg atcctacgcc gttcagacca
    26821 acggatttct taaaggcagt agagtcgtat tttgctccgg tattgatttt tgatacgcat
    26881 tctacaactt ttcctaatgg aatcccgcgt ccataatcgc ggatggtaac ggtcttgtcg
    26941 cttatatcaa tgtctattct cctgccattt cccatgacaa actcgtcaat agagttgtca
    27001 attatctctt tgattaacac ataaataccg tcgtcctgcg aggagccatc gcccagcttg
    27061 ccgatataca ttccgggccg ggtttgaata tgctccttcc agtcgagcgt gcgtatttga
    27121 tcttcaccgt attcgaaaga catttttgta aaaatttttg caaaagtagt gttttttgag
    27181 cttaaaagca aagcccagcc tgaaagttat gcacatgtac ttgtgcataa atcaatggaa
    27241 aatgatttaa ttcatggtaa aaatgtttga gttaagatag tgtttatctt ttgagataat
    27301 cttaaattct aaaaacatta aatcatatat ttttgcctat aaaatatctt ttaagtaaat
    27361 gtattatcca tgaaatcaaa agtttttaca attagtttgt tgtttttctg ctttacggca
    27421 ttaggacaaa ataacctgaa tgtagctttc ggcaaccttg tcaaacttca aaagaatcct
    27481 gcaaagcagg agtttacatt aatttccgat aatggtacag cgaagctaac ggtttattct
    27541 ccaaacataa ttaggataag gattgcaaaa gagttcaatt ctgatttttc atattccgtg
    27601 gttgcaaacc cgatgaaatg caactttgat tacggtgagg ataataataa cttttttgtt
    27661 agcactgatt ctctttttct tgaaattagc aaaaagccta ttcgatttag ttttaaaacc
    27721 cttgatggtc aggtggttaa tcaggatgat ccggcttttg gagtgtcttg gataagaaat
    27781 gaggtaacta attataagtt gattcagggg gatgagaaat ttatcggcct cggtgaaaaa
    27841 accggtaacc tgaatcgtag gggtgaggcc tatgaacact ggaataccga taaccctcac
    27901 tacgataata attctgatcc gctttatgca tcaatcccat tttacattgg aattcacagc
    27961 ggcttgaact atggtatttt tttggataat acacatcgaa ccactttcaa ctttggcgct
    28021 tccaacgatc gtttttctta tttctcggca gaagatgggg aaatgaacta ctatttcatt
    28081 tatcataaaa atgtaacaga cattatcagt tcatttacgt ggctcacagg gcgaatggag
    28141 atgcctcctg tttggtcgct cggatatcaa caatgccgtt ggagttatac acctgaatcg
    28201 gaagtgatgc gtattgcaaa cacttttaga gaaaagaaaa ttccggctga tgtgatttat
    28261 cttgatatac accatatgga caattataaa atatttacct ggaatccagt agatttccct
    28321 caaccgaaaa agatgataaa gagtttggcg gaaatgaatt ttaaaacagc aataattgtt
    28381 gatccgggaa taaaagtaga aaaaggttat atggcttatg atgagggagt tgcgtataag
    28441 cattttgtaa aatatccaga tggttctaat tggacagcgc aggtttggcc aggatggtgt
    28501 cattttcctg attttacctc tgaaaagagt agaaaatggt ggggagagaa atttaaaagt
    28561 tatgtagaag atggtgtcgt aggtttttgg aacgatatga acgaaatagc tacatgggga
    28621 caacaggttc ctaatttaat tgagttcgac tgggatggga acaaagcgac atatcggcag
    28681 gcaaaaaatg tttatggtat gttgatggca cgctcaactt ttgaaggcgt taaaaagctg
    28741 atgaatggta agcgcctgat gaatatcact cgttccgggt ttgctggtat gcaacgttat
    28801 actattctct ggacaggcga caatcaggcc accgatcatc atatgatgct tggcgtaagg
    28861 ttagtaaaca gcctcggatt atcgggatta tcttttaccg gatcagatgt tggaggattt
    28921 ggaggaaatt gtactccggc tctctttgca cgatggatgc aaattggcgc ttttacaccg
    28981 tttttcagag gccattccgc ttataatact atcagccagg agccttgggt ctttggcgaa
    29041 acagttgagg agatatcgaa aaactacata cagctccgct ataatcttct tccatatatt
    29101 tatagttcaa tgcactcgtc tgttatcagc ggaatacctt taaaccgtag cttggctata
    29161 aattatacaa tggatgaaac gatttatcag gaggagtacc aaaatcaata tctatttggc
    29221 ccctcaatgt tggttattcc ggttgaaagt aataaggaga tcgtaaaagc atatcttcca
    29281 aaaggcgaat ggtatgactt ttataccgat caatattttg atgggcaaca agaggttttt
    29341 gccgagtcgc cgcaggacaa attgcctgta tatgttttgg caggcagtat aatcccaatg
    29401 caatcgccaa ttcaacatac cgctgaaatg ccttccgaca cgctgtacgt ccatctttat
    29461 aaaagtcaga gtaataagga tattgcgtgg aaatattatg aggacgatgg cgttagttat
    29521 gattatcaaa atggaaaatt ttatttacgg gagataattt ataagccaga gataaatgaa
    29581 gttgagtttc gtaaacccga tggtgatttc caaagtaaat ttaaaaatgt cagattggtt
    29641 tttcacggat ttgacgagct tgactggatc attaaattcg atgggaagcc tcggaaggtt
    29701 tatcctttat caatgaattt tcgaaaagta tccgaaattc caccagaggg tgattattct
    29761 acaattacat gccctacgat tgtattccct aatgatgaag agaggatgat aattacatgg
    29821 taatggaata attttgagga tttgaaagat cggataatta taccggataa ttataatgga
    29881 caatctgtaa tagtttagtt taatttaact ttgcgggaaa ataattcagc aaatgaccat
    29941 tgacgaaatt atcaaagcgg ctttagcgga agatgtggga aacggggatc atacctcttt
    30001 gtcaactatt ccacagtggg caatcggtcg tgctaactta ctgataaaag aaaatgggat
    30061 actttgtggt gtggatatag cccgtaaagt gttcaacact gttgatcctg atcttgattt
    30121 tcaggtgttt atcgaagatg gtaatgacgt tgttccgggc gatatagtat tttctgtttc
    30181 tggaaaagtg atatcaatcc ttactggcga acggctggcg cttaacttta tgcaacgatt
    30241 gagcggaata gcgacaaata cacgtctgat ggttaacgag ttgaagggta ctaaagcgcg
    30301 cttgctcgac actcgaaaaa caactccaaa ttttcgtgaa cttgaaaaaa tggccgtgag
    30361 aacgggcgga ggcgaaaatc atagaatggg attatatgac atgatattaa taaaagataa
    30421 tcatgttgat tttgcgggtg gaataaaaca agccattaaa gcggtgcata actatataaa
    30481 ggataaagct ttagatttga aaatagaaat tgaagtcagg aattttgatg aactgaattt
    30541 agtgatggaa acaggaggtg ttgatagaat catgctcgat aattttaatg tatcagattt
    30601 gaaaaaggca gtggagcttg ttgccggtcg ttttgaaaca gaagcctcgg gaaatattac
    30661 tctgaaaaat ataagagaat atgcaaaaac gggggttgat tttatttcat caggccaact
    30721 gactcatcag atcagaagct tagatatgag cctgaaagcc gatttttaat taaaagataa
    30781 gaaattgcta gaccataaaa ataagctact atctaagtta aatcctaagg ttgttaagtt
    30841 tcttcgcaac tcacttgcat ttattatcag gattttacga aaaattacta tcccaggcac
    30901 taataagatg ccactgtacg acgtgatgat tttttttctg aaaggcttaa ctgagggaag
    30961 gctaacttta agagcatcat ctatctcttt cgattttttt ctggcgcttt ttccggtaat
    31021 catcgttttt tttactctta ttccatttat tcctattaca ggatttcagg aagaattgtt
    31081 acattttctt gaggatgtta ttccttcaac attgtggata catgtttctg aaacccttga
    31141 agacatcatt gttcagcctc gttcaggatt attgtcgctc ggttttttac tcgcgctttt
    31201 cttttccaca aatggggtaa actcaattat agaagggttc aactcatcat atcatacaac
    31261 tgaaaaaaga ccgtatttaa agcagcggtt ggttagcctt tacattgttt tagtgcagtc
    31321 ggtcttagtg attttagctg tttcgctgtt tttgataggc ggcaggattt tgcaattttt
    31381 agtattccac ggaatcctca caaatcagtt gactattgtt atccttcata tcgttaggtg
    31441 gctgatgatt gtagggttgt ttttagtctc agtctcattt ttgtattatt ttgctccagc
    31501 taaaaagaag gagtacaaat ttttttcaat tggtgcaata tttacatcag ggatgatgat
    31561 tctgaccacc tatgggttca acttttatat cgaaaatttc aaccgctata atgctcttta
    31621 cggctcaata ggaactcttt tggtttttct tctgtggtta tatttcaact ctatcatcat
    31681 tcttattggc tttgagctga atattagcat taatactgcc aaaaaaagat ttgaaaatgc
    31741 ttaaccatct gcgaaatacg gtaaagcata cggcaattta cagcctgggg aatctaagta
    31801 caaagattat tggattgatt ttattgccgt tatatacatc ttatctaact actgctgaat
    31861 atggtatttt ttctatttta gaagtaacaa gtcagttttt tacagccgta gttagcttgg
    31921 ggcttactgc tggtatgatg cgttggtatg cagtagaaga tgatgagcag aaacgcaaaa
    31981 taatcgtatt tacgacatgg gtaactactt ttatattagc tttatttgta aatgttgctt
    32041 tttaccctgc aaacaaatgg ttttcgtcta tattttttag tcatgataat tacgccgatt
    32101 attttcttat cctttggtta tgggtggggc ttgaggtagt aaaccgaata ccttttgagc
    32161 ttttaaggtt aaaagaaaaa tctttgctat ttgttacact ggttataatc aagtttgttc
    32221 ttatccttgt cttaaatatt tacttcattg tttatatgaa aatgggagta aaagggataa
    32281 tccttagcca ggtaataggc aaccttttag tttcaatttt tactttgccc tttttgttac
    32341 gtaacatatt tcttaaattc agcttaacaa tactgaaaga gatcagcctg tttagtattc
    32401 cattggttct ttcctcagtt gccacgatgg catttgcgct tagcgatcgt tacctgataa
    32461 gctattatct taattattcc gaagtgggga tttactcctt aggatataag gtggccagtg
    32521 ttataaatct gtttttggtt cagtcatttt tgcttggctt tgcaccgctc gcatataaaa
    32581 ttttcgacaa gccagaggcg aagcgttatt ttgcacgtgt taccacctac tttacttttg
    32641 caatgattgc tttagcgctg ttgcttatct tttacgccaa agaggttatc gctcttttct
    32701 ccagtggaaa taaagatttt gtactctctt ttaatgtgat cccacttctt tgtgttgcaa
    32761 tgatttttag aggattaaca gctatagtat caatggggct acattatgta aaaaagacaa
    32821 aatataacct tataatagtt gtaattgttg ctgttttcaa tatcgggatc aactttattc
    32881 ttataccgtt atttggtatt tacggagcgg cggttgcatc aatacttgca aacatgttga
    32941 tgacgatatt gttctatgtt tactcacagc gttattattt cattagttac gaatttgtac
    33001 ggataattaa gctattttta ttcggtattg ttttcttgtt cctggcttca tttgcaacag
    33061 tatttggctt ttggggaggc ataatattca aaactttttt actcctttta tttccattag
    33121 ctttgactat agtaaaattt tatcagtagt gtccactaaa agttaaaaaa catgattata
    33181 attaactaag cagat
//
LOCUS       DAOOAI010000012        28147 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_2207_length_28147_cov_4.996333, whole genome shotgun sequence.
ACCESSION   DAOOAI010000012 DAOOAI010000000
VERSION     DAOOAI010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 28147)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28147)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28147
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2207_length_28147_cov_4.996333"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="PK345_01815"
     CDS             complement(<1..232)
                     /locus_tag="PK345_01815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33791.1"
                     /translation="MANLNISNLGIYTLLIVALILFIALRLLTWLFPLIITKTERRKI
                     ALRYKSLCELLIWVLFLLWAVQFLYTSNQPFAY"
     gene            complement(243..5429)
                     /locus_tag="PK345_01820"
     CDS             complement(243..5429)
                     /locus_tag="PK345_01820"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33792.1"
                     /translation="MSQAKKFGTFTGVFTPSFLSIIGVIMYLRLGWIVGEAGIFKALA
                     IILLAHVISITTGLSISSIATDKKIKTGGIYYMLSRSLGLPMGGAIGITLFIGMSLSI
                     ALYLIGFAENFLGVEWIRTFFGLGTTLNDIRIVGTAAILILLIIVFISTSLALRSQFV
                     ILTTIIISLIAIVLSLIFRVDVRPDHIAFGARPDGLPFEAIFAIFFPAVTGFTVGVAM
                     SGDLKDPKKSIPKGTMLAILVSMVIYLSLAVMFGCFVDRQILLNDYNFLSNIAFWAPL
                     VIAGVWAATLSSGLGNLLGAPRVMQAIARDKLMPKVFGKGVGVNNEPRNALILSFIIA
                     QIAILIGDLNVVARVVSMFYMVAYGFINLSYVLENWASPDFRPSFRIPGWIGIIGFIA
                     SFAIMFKLDALAMTIAILLMFLLYFFLSKKDLKLDYGDVWQSVWSSVVRSSLIQIARK
                     GIEERNWQPNIILFSGHSESRPHLIEFGKQLIGNQGLLSNFDLIKTEISDPIKPRHKQ
                     SLPFDNKDSIKGFFSRQHFCSDLYEGIRTISSVYGFSGIEPNTVILGWGRQSKDPEKF
                     VQTINYISALDLNVLLMDYDKKVGFGNYKQIDIWWRTTEHNGNLGLVLQKFLKSSDKW
                     LKAKTRILIVNPINDQKHIIYWKTEDILDNMRIKAEIKVINNQIEKRSFYEIVQAEST
                     HSDLIFLGLPNIVSGKEKEFVEETNALCQDIGTVILLKASTTFKELNIGAKNIQISQS
                     ETLPYTEEKTFEPVIQTEFLEIPGKIELSIELKSLHEKLLNLAKHQNSEYYNRLFDQQ
                     NSLLDNVAKSIEKHFSGLLKKIEDTNDGNINILFSKYNANLWFRLRRILNDYRDETLI
                     IIEKTLQESITNYREEIDKIIISSPSYIRIPLNKFDLRSYVDDPLSEKYIKSKIKFLK
                     LLSSNKESEHLLAYQKILVRFTQQKLNALHQDLMESMGSDLSHLLMMIKSISSEIYDN
                     LNIYLSEKDLKNPEQLINKKKLEIAERLQLMMKINTENHAEISQNIIDKTAKLALDLN
                     KAFSVFRSNNYHLKHFRQSSISKNMIDELPAKTTKNQKLIVDYMLLELSLFSVQSRFR
                     NAFLEAEDDIEQVFDEQIIQRQKEIKKFLNDYSNKLITNPKQGFNLVNVPELEHSAMI
                     QELFNQLIQHTFNKIKEASKILPEQVEVIDNASIIDFQDIQFQQIQTKTIYVSRMLDF
                     LLQSEFADEVKESISNISEKLLRLKTATNEIPRTLDFILNPEVIKKNEKKLTTEEKIT
                     LIKRHAELIDVEITNALELKNNIVQIFRNRFNTIGEKLTLYSFVDNAGMMDSKIRQKE
                     IKKGASMLRKIRNSAAEFIQKQINQYWYSQSIGTIFRHRLKSELDRNRFRINEALEIL
                     DKVSIDQEIDNKLPYFYKQLFIRKQYYLSELWTGRERELNEAEKAVRRYRSGIMGGIL
                     VVGDHFSGKTFFSHQFINKFYPDANTFILTPPYAGSTDVSLFKKSLESALEVSGSYYK
                     MFNSLPGNSVIIIDDLALWWEQTETGFVVVSQLIELINKYSRQSLFVINLNRFSYELM
                     RRMYPIDNYFISIIELSPFSAAELQKIIMKRHNSTTLNLRMYSGESLRSWDYARIFAK
                     LASISGGNVGIALHAWLSNIADVKEDTIYIRIPQSPDASVLDNLNPRWYLILIQLMMH
                     KRANLQKLARICRLNTHEIKETIDILNRSGIISEKNPGVYEVTITFYPFVQQKLIEKG
                     ML"
     gene            5891..9511
                     /locus_tag="PK345_01825"
     CDS             5891..9511
                     /locus_tag="PK345_01825"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPX33793.1"
                     /translation="MKKTFSLLFVIILYASTVFSQSLPRMFHFSDDSLRLIRGGVEST
                     GLYDEYVIDTIWLSFSQPNYWQLLTSNYNSQTNIPATLVYKGATFDSVGVRFRGMTSY
                     FFVQGQKKSFNIELDWVHNDQHIEGYETLKLLNSYGDPSILREALYLKYSREHIASAR
                     GNHVQLFINDQNWGIYVNVQKFDRKHVKEWFVDADATRWRAEKVGGGGGFGAGKSSLN
                     WLGWDTTTYKQYYQIKKYYKPNPWDDLVNTCYVLNNTPSNLLMDSLANYLDIDGSLWF
                     LAHEILFTDDDSYIEKGGMDYYLYFDVGSNRMIPIEYDGNSCMSVQKASQWSPFYKEN
                     NTAYPLMNKLMAVPEIRQRYLAHIRTLMETTFDLPVIQGLIDDWAARISTNVQNDPKK
                     IYSFTQFQSELSKLKQFCSTRRNFILNNTEVNRTGPAISNVIYSVEGQNFIKPDPFQP
                     VDITANVTATQGVHKVILYYGTGLIGKFTRVEMFDDGNHNDGVAGDGIYGAQIPAHAS
                     NQWVRFYIEALANDTWKTAKYEPQGAEHDVYIYQVKPGQILDSPVRINELMSNNVSAV
                     TDPAGEYDDWVELFNTTAEPVDLSGYHLSDKQNQLDLWQFPQGTIINGNSYLIVWCDN
                     DLGQEGLHANFALSKDGEQLYLSTPNLLVADEVVFGPSDPDMAYARVPNGTGNFVWQE
                     PTFNGPNDLPFFSPMVINELMPNNTNSIQDPDGEYDDWIEFYNFSSDTCDLSGYYLSD
                     NANQLNKWQFPAGTTVQGHGYLIVWCDNDLTQQGLHTNFSLNNSSGAIYLSGPDMEPV
                     DEVVYASGSPDIAYARVPNGVGDFVWQQHTFNDHNGPFIECDVVVNELMADNVSAVQD
                     PDDEYDDWVELYNNSSQPFDLSGFYLSDKINEPNRWEFPEGTVIEGNGYLIVWCDNDL
                     NQEGLHANFSLSKNGENVILASPYIQIIDQVTYGFAPTDTAYARVPNGVGNFIWQHHT
                     FNGPNNIEFDCQVVINELMPDNTQSVQDPNGEYDDWIEFYNNSTEPCDLSGCYLTDDY
                     YVLNKWQFPEGTVIEGNGYLIVWCDNDLGQEGLHANFTLDNNSGALYFSDPFQQIVDE
                     VVYEGASPDSAYARLPNGVGEFVWKAHTFNLTNDYGINVEERQAFDGLFAIFPNPATK
                     KLTVIVNDDDPVLITIYNLLGEKVLQVWVDHQMEINTAEFHRGIYFVHKQFGGMEKLI
                     LR"
     gene            9705..10850
                     /locus_tag="PK345_01830"
     CDS             9705..10850
                     /locus_tag="PK345_01830"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="THUMP domain-containing protein"
                     /protein_id="HPX33794.1"
                     /translation="MIAKTMVGLEETLFNEIVAAGGEKVRLLNRAISFNGDTACMYRV
                     NYTCRTALRVLKIIAEFSVNNEDELYNEIFKVNWSDYLNVEQTFAVDAILSKASITNS
                     HYAAQKVKDAVVDQFRKKEGNRPSVRISNPDVLINLHLTGNWCTLSLDSSGESLHKRG
                     YRKWQGDTPISELLAAGMIMIAGWNGQNPFYDPMCGSGTLLIEAAMIAAKIPSGYHRK
                     SYGFMNWLDFDNDTWTKVKDEANGKIVEIKQHIIGSDVNQKTLSLMKQNVAAAGFDDV
                     ILLKQAAFEDSSPLVNQSGTIITNPPYGERLQKRDIKSFYKTIGDVLKQKYAGWDAWL
                     ITSDYEALKSVGLRTSRKIKLFNGPLECRFVKYEMYQGSRKKVIPED"
     gene            complement(10847..11866)
                     /locus_tag="PK345_01835"
     CDS             complement(10847..11866)
                     /locus_tag="PK345_01835"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HPX33795.1"
                     /translation="MTKIKAAITGIHCYVPPYVLTNDELATMVDTNEEWIMTRTGIKT
                     RHILKGEGKGTSDMGVEAVKGLLKKTNTNPDEIDLMICSTVTPDMQFPATANIIADKV
                     GIKNAFHFDINAACSGQIYSLTTAAMYIETGMCKKVIMVGADKMSSIVDYTDRTTCVL
                     FGDAAGAILLEPTTEDVGIIDSIHQSDGSGRIHLHQKAGGSVKPASHETVDAREHYIY
                     QEGQPVFKFAVSKMADVAVEMMTKHNIRPEDLAWLVPHQANMRIIEATARRMGIQKEQ
                     VMINIERYGNTTSATIPLCIWEWEKQLKKGDNLILAAFGGGFTWGAIYLKWAYDGKAA
                     ASIEV"
     gene            complement(11884..12834)
                     /gene="plsX"
                     /locus_tag="PK345_01840"
     CDS             complement(11884..12834)
                     /gene="plsX"
                     /locus_tag="PK345_01840"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HPX33796.1"
                     /translation="MRIGLDVMGGDFAPEVTIKGALLSLDELPHDVQLVLIGDEAFLT
                     TKLSDLNIPPGRIETVYSSEVIESGEKPLKAISTKQNSSIVIGLRMLRKGEIDAFSSA
                     GNSGAMLVGSMYSVGPIKGVIRPTTFAHIPQENGNTSILLDIGTNTDTKADVLSQFAH
                     LGFIYAQEVMGIEKPRVSLLNIGVEKGKGNLLTQSAYEMMHESKEYFFVGNIESRDLF
                     NNKADVIVCDGFTGNILLKQIEAFYRIMQKRNLLDDYFERFNYENFGGSPILGLNGTV
                     VLGHGISSAIAIKNMLKLSYNIATTRLSDKVKEALYKYSN"
     gene            complement(12875..13063)
                     /gene="rpmF"
                     /locus_tag="PK345_01845"
     CDS             complement(12875..13063)
                     /gene="rpmF"
                     /locus_tag="PK345_01845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HPX33797.1"
                     /translation="MAHPKRKVSKARRDKRRTHDKATMPTLAKCPVTGTVHIYHRAYW
                     VDGDLYYKGKLLIQKTEA"
     gene            complement(13098..13616)
                     /locus_tag="PK345_01850"
     CDS             complement(13098..13616)
                     /locus_tag="PK345_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HPX33798.1"
                     /translation="MDYLREYNIEFASLKVGIHHFNYEIGSKFFEAIDYSEFKKGELK
                     VNLELDRQPRMLVFKIDIKGYVEKECDRCLDVINYPVNVVHKLIFKFGDQWEDVSDEI
                     IIIPETEHQLNMAHYLYEFISLSLPMQCIHPDDENGNSTCNKEMLILLSQHSKNREVD
                     PRWEILKKLNDK"
     gene            14067..14846
                     /locus_tag="PK345_01855"
     CDS             14067..14846
                     /locus_tag="PK345_01855"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HPX33799.1"
                     /translation="MELFYRHYGNGQPFIVLHGVFGVSDNWVSFARRMASDFEIFILD
                     QRNHGRSPHHPVHNYFAMCYDLKEFIDRQQIRNPIILGHSMGGKVAMLFALKNPEIPK
                     ALIIADVSLRSYHKQQLHLSMIDAMMSVDFDKAETRDDIARQLSVAIKNQRILQFVMK
                     NLYRRQSGQFAWKPNLEAINSNMDEILDGIESKNVFTKPALFIRGGQSDYITYDDFDQ
                     IYRNFSKAEIETIENAGHWLHADQPEEFEKKIRRFLTQTDQ"
     gene            complement(15103..15861)
                     /locus_tag="PK345_01860"
     CDS             complement(15103..15861)
                     /locus_tag="PK345_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33800.1"
                     /translation="MAKKKIVHVGGNNSSNIGTTETFKPTAESKSKATRLRVISAILW
                     LLAIGAQVVAISLLFRQPINMMWIIILIVIDLALAITGSILWKKSNRLDPASEKNKFL
                     FFMQSQLGLVVAIIAFLPLVIFILTNKNLDAKQKGILGGIAGLALIIAGVTGIDFNPP
                     SVEQYTEQTQRVEELTGKNEVYWTKSGTKYHIYEDCHAINKDVTTEIFQGTVAQAREL
                     KNITELCKFCENRAAKEVESDIKQEKLLEIEKEE"
     gene            16184..19129
                     /locus_tag="PK345_01865"
     CDS             16184..19129
                     /locus_tag="PK345_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HPX33801.1"
                     /translation="MSESYILHQLKKFRNQIEGDLLKDYSSRILYATDASAYRELPLA
                     VIKPRNKEDIRKLVLFAGKHGVSLIPRGAGTSLAGQVVGNGIVVDVTRYLNKIIEINP
                     EERWVRVEPGVVLDELNIALARHDLFFGPETSSSNRCVIGGMVGNNSCGAHSLIYGST
                     RDHTLEIEAILSDGSMVKFKALSKEEFQQKQIGETLENKLYRNITDILSLPLNKEEIR
                     KEYPDPMVKRRNTGYAIDLLLESEPFSESDVRFNFCKLLAGSEGTLAFITEIKLNLVP
                     LPPKVKGLICVHFNSLEEAFQANLIALKYMPGAVELMDNVILQQTKNNLEQARNRFFV
                     KGDPAAILIIEWARETKEEIDDVAEKMELDMRGAGFGYHFPRIFGSDISRVWALRKAG
                     LGVLSNIAGDAKPVSLVEDTAVNPKVLPNYLAEFQQLLDRYGLKAVYHAHIATGELHL
                     RPVLNLKDPKDVELFHTIAFETAKLVKKYRGSLSGEHGDGRLRGEFIPLMIGQHNYNL
                     LKEIKQTWDPNNIFNPGKIIDTPSMKTNLRYDPGKKTRKIRTYFNFSDDQGVMRAVEK
                     CNGSGDCRKTEIIGGTMCPSFMATRNEKNSTRARANILREFLTLSPKKNPFDHSEIYD
                     IMDLCVSCKGCKSECPSSVDMARYKAEFLQHWWDKHGIPLRTRAVAYISSLNRIGMLF
                     PAITNYLFTNKFIANLFKRVLGIAPKRSIPLLYRYTLSRWLKRNKHKLLPHRKPIGSV
                     YLFIDEFTEYNDTEIGIKAVELLTRLGYEVKIAVHKESGRAFISKGLIRKAARLANFN
                     VTTFKNIIDAQTPLVGIEPSGILSFRDEYPDLVDPELRPEAKLLAQNALMFDEFFEKE
                     IKAGKISRDLFTMEHRKIKLHGHCHQKSLASIEPTKAMLSLPENFQVEEIKSGCCGMA
                     GSFGYETEHYELSMQIGELVLFPEVRKASTEVIIAAPGTSCRHQIKDGTGRTAYHPIE
                     IMYQALLKK"
     gene            19303..19776
                     /locus_tag="PK345_01870"
     CDS             19303..19776
                     /locus_tag="PK345_01870"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX33802.1"
                     /translation="MQQDQALQITVKEASPEDQEMIIGFQIDMAWETEKVKLDENTIR
                     LGVQNVFKNPNLGRYYVAKANNLIVASLLITYEWSDWRNSIVLWIQSVYVIPKYRKRG
                     VFRKMYENIKQQVERDNEITGIRLYMVHNNRIADHVYEKLGMDGNRYRLFEWLKK"
     gene            20377..21471
                     /locus_tag="PK345_01875"
     CDS             20377..21471
                     /locus_tag="PK345_01875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33803.1"
                     /translation="MEEKYKQYEKTIKKKHSFAWTPKYASEFSTRLSAKEFIAIAEKT
                     FKDLGWDIVYQDDKSIEAKRSEKSLGTERWTEGITATFDYGKIQVKSVTLGNEMWDVG
                     RNSKRVQLFIYAFQEIEKSFDREELQNLVNEKETVNNWDDYVVPDSLPKPKEIREPKF
                     YIHIVGCSILSILIAFLLAKVSISGFYIIGLFEFLVGFAIGFTFKYLIKFSNFTNYDK
                     LHYVILGTIGLTYLLNQYFQYEIILYENNYDRIGFFEFLKIKFEQGLTIKTLNTGWIG
                     LVIHWILQLVITYYVTVMAVIRNLTKYQLDRVQPEVVDFAFYHFVKDKTEDQIRKELA
                     LKGWTETQNQDEVFEAIGALQTTQDVNRMQ"
     gene            21468..21617
                     /locus_tag="PK345_01880"
     CDS             21468..21617
                     /locus_tag="PK345_01880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33804.1"
                     /translation="MKRNTSANSKALHNGGVETDIIVCGFYVTLSWLDRVVFKNPPLR
                     QAPNR"
     gene            22088..22486
                     /locus_tag="PK345_01885"
     CDS             22088..22486
                     /locus_tag="PK345_01885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33805.1"
                     /translation="MSLGIGLFAFLLIPNTINDWKKNNIKSVLLVISISIFFFFSFYI
                     DSLFQDIELAVTMGFIGLLLALGYLFLRNFWVVYSALFIIMLVNTLADNKYDEYSYWV
                     VIIGSLLSLTILTFDFIKNNRIKITNLSIE"
     gene            22490..23539
                     /locus_tag="PK345_01890"
     CDS             22490..23539
                     /locus_tag="PK345_01890"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPX33806.1"
                     /translation="MTTEKEKSLQAHLDKVVDGKKVFGTSFAFKKDTETWQGASGNLT
                     TDRPYFIASTTKLFTTAIIMKLRAEGKLSLEDKISKYIDASILSGLHFYKGKDYSQEL
                     TIRHLLSHTSGLSDYFQDKGASGKSLENELMAGNDQFWTFEESIDRSKKMSPLFVPGT
                     KGKAKYSDANFQLLGKIIETITGKSYAENCQDRIIQPLSLTKTYLYQDATDEKPKMLY
                     HKSKELKIPKAMTSFGPDGGMVSVSADMLVFIEAFFTGKLFPLSYIDELQEWNRIFPP
                     MRAGVGIHLFKLPWIFDPTGAIPYFIGHSGLSGALAFYSPKENIYIAGTVNQTAHPDI
                     SYRTMISLTQRILKK"
     gene            23536..24102
                     /locus_tag="PK345_01895"
     CDS             23536..24102
                     /locus_tag="PK345_01895"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HPX33807.1"
                     /translation="MKDRNNSVTIFNARIIGIFFLLAFLAYGIGRQLFESADKPEKYL
                     GALLILANSAMVLFIGILLRKTLTRYNPIVGNIYFFTRIFEALALSTILLKGVVSDDL
                     GYFLAMLVLGIGSIPMCLTLYKHKVSPSWLAIWGAIGYAVFAFGFLMELFGKEWSIYF
                     LGPGGLWEVVFAIWLIVCGSKNIKPTTR"
     gene            24162..24857
                     /locus_tag="PK345_01900"
     CDS             24162..24857
                     /locus_tag="PK345_01900"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPX33808.1"
                     /translation="MKISKRMIYGILLTAIVFLLATLAGRNINLNINFLPYSFVTHTT
                     LLILSITLICGLKKHVKYNMAWPKFKKTLKPILFGFLAAIIVNTFMVALTKGLGGEVE
                     SHFAFDLMSPLQVFLFIFIYASVAEEVLFRGFLQNILKPLKNKGIKLFKRHISVPVMI
                     GAVAFSLAHLILITSGANTFFIVRTLVFTFVLGLIAGYYQEKYDNNAYAIFVHMAGNF
                     MGVVAAILTSLSV"
     gene            24890..25714
                     /locus_tag="PK345_01905"
     CDS             24890..25714
                     /locus_tag="PK345_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33809.1"
                     /translation="MKRILSVLLVLLIFTSCTDMKSVFIKDKSELIERNKAHKIEVFT
                     EELIKDLPEPLKKYLRICGYVNTPVPINANVYWAESWLKMSPEKEWRKLHTTQFNSVK
                     PIGRSAYMKILGMPVAARDLYRDGYGEMNVKLLNLIKVAFDNSKEVAQSALITTFCEF
                     MLIPGYLLLDNVKWEQIDDYSIRGTVVDNGIEVSGIFYFNEEGLFTHFETFDRYFTTG
                     KNSYKKVKFSVVVESYKTQYKLKIGEKVKVMWHLPEGDYEYYKGTIDRIEFNVVEY"
     gene            25946..26842
                     /locus_tag="PK345_01910"
     CDS             25946..26842
                     /locus_tag="PK345_01910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33810.1"
                     /translation="MKSLKIYQFKGIVFIMFLIFSSNLFTGCKKDVLVSNDDLQPIMT
                     FENIKVKVLEDRLYFENRADYAEAIEILSNIEESTFDLFEKSIGFQSMRTVLNQKERE
                     AINIEDELLATLLNPQGIIQIAENIFEIDALNEIVTVYSAPETREKNGKRTFSTNDDV
                     FDRLDGIEALYTKSGCPSYKEKVKNWAVWGGNVECKVVYQKAGIYFSLQSKIKKDHWG
                     GSAYISLTCPSGSSYTQNRSGASQQSIPSNSQGGYGRVYNYRPYSSMRGLKQYYFSVN
                     FYCDDSVSGYGPYSTTLTINCN"
     gene            26895..27797
                     /locus_tag="PK345_01915"
     CDS             26895..27797
                     /locus_tag="PK345_01915"
                     /inference="COORDINATES: protein motif:HMM:NF025420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4249 domain-containing protein"
                     /protein_id="HPX33811.1"
                     /translation="MRLICFGCLFFLIFGCTKEINIELPMQDSKIVVNSLFTNDAPLS
                     VYLTKTRTPFNEKELFIENARVIIYSENITDTLQYMGEGIYMSSHLGLANSEYRIDVI
                     SNGFEKVTARDYIPKQMALPLIVSGFRDSVGVNEHGDYYSQFTITFLDDANAKNNYEI
                     FIKHLLFEDEVWDRGCFSNDPIIVAEGLNDYFPNTVVFSDAFISGKEKELAINYYPVT
                     YSKPTGGQGELVDPNYKVIVYFRNTSENYYKYKKKLAVHLAGQEFDFWAGSGEPVQMF
                     SNVVGGYGIFAGYTERIDTIQKLK"
     gene            27804..28088
                     /locus_tag="PK345_01920"
     CDS             27804..28088
                     /locus_tag="PK345_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33812.1"
                     /translation="MKKNIILLLFAFLPILVIAQEVKRNKSIEIIAGYANYNYNLNGV
                     FLSAGCSRGFNKYLYLTGSLTLSNGSRNRDNSFVNDQFHAAALQIGLRGV"
BASE COUNT         8808 a         4851 c         5541 g         8947 t
ORIGIN      
        1 catatgcaaa aggttggttg gaggtgtaaa gaaattgtac tgcccataac aggaacaaaa
       61 cccatattag cagctcgcat aaggatttgt agcgcagcgc aatttttcgc cgttcggttt
      121 tagttattat tagtgggaag agccaagtga gtaaccggag ggcgatgaag agtatcagcg
      181 ccactatcaa caaagtataa ataccaaggt ttgaaatatt taaatttgcc atgatttgtc
      241 gtttataaca ttcctttttc gataagtttt tgttgtacaa agggataaaa ggtgatagtt
      301 acctcatata caccaggatt tttctcggaa atgatacctg atcggtttag gatatctatg
      361 gtttctttta tttcgtgtgt gttaaggcgg catatacgtg ccaacttttg caggtttgcg
      421 cgcttatgca tcatcagttg tatcaatatc agataccatc gtgggtttag attatccaac
      481 actgatgcat cagggctttg tggtatgcgg atataaatcg tatcctcttt tacatcggca
      541 atattagata accatgcatg aagcgctatt ccaacgttac cgcccgagat gcttgcgagt
      601 tttgcaaaga ttctcgcata atcccatgag cgaagactct cgccactata cattctaaga
      661 ttcagtgttg ttgaattatg gcgtttcatg ataattttct gaagctcggc agcgctaaat
      721 gggcttagtt caataatgct gatgaaataa ttgtcaatcg gatacattcg acgcattaat
      781 tcatagctga accggtttag gttgatcaca aaaagagatt gacggctata cttattaata
      841 agctcaatca actgacttac aacgacaaag ccggtttcgg tctgttccca ccaaagagcc
      901 agatcatcaa taattattac gctgttgccc ggcaatgaat taaacatttt gtaatacgac
      961 cccgacacct caagagcgct ttcaagcgat tttttgaaaa gacttacatc tgtagagccg
     1021 gcataaggcg gtgttaagat gaaagtattt gcatcgggat aaaacttgtt gatgaactga
     1081 tgtgaaaaaa atgttttacc cgaaaaatga tcccctacta ctaatatgcc tcccatgatc
     1141 ccggatcggt aacgccgtac tgccttttcg gcttcgttaa gttccctctc cctgcccgtc
     1201 cacaattcgc tgagatagta ttgcttacgt ataaaaagct gtttgtaaaa atagggtaac
     1261 ttattgtcaa tctcttgatc aattgatact ttgtctaata tttcaagagc ttcgtttatc
     1321 ctgaaacgat ttctgtcgag ttcacttttt aatctgtgcc tgaatatggt accgatgctt
     1381 tggctgtacc agtattgatt aatctgtttt tgtatgaact ccgccgctga gtttctaatc
     1441 tttctcaaca tggaggcgcc tttttttatc tctttctgcc tgattttgga atccatcata
     1501 ccggcattat caacaaagga gtacagcgta agcttttctc cgattgtgtt aaagcgattc
     1561 ctgaatatct ggacgatatt gtttttgagt tctaaagcat ttgttatctc cacatcaatc
     1621 agttcggcat gtctctttat cagagttatt ttttcctctg tagtcagctt tttttcattt
     1681 ttctttatca cttcaggatt taaaatgaag tcaagcgtgc gtggtatctc attggtagct
     1741 gtttttaagc gcaacagctt ttccgatatg ttactgatag attcttttac ttcatcggca
     1801 aattcgcttt gcaacaggaa gtcgagcata cgcgacacat aaatagtttt cgtttgtatc
     1861 tgctgaaatt gaatatcctg aaaatctatt attgatgcat tgtcaataac ctcaacctgt
     1921 tcgggtagta ttttgctagc ctctttaatc ttgttgaagg tgtgctgtat caattgattg
     1981 aaaagctcct gaatcatggc gctatgttcc agctcaggga catttacaag gttgaatccc
     2041 tgctttggat tagtgattag tttgttacta taatcgttaa gaaatttctt tatctctttc
     2101 tgccgttgaa taatttgctc gtcaaatacc tgttcaatat cgtcttcagc ttctaaaaat
     2161 gcatttctga atctcgattg taccgaaaaa aggctaagct caagcaacat ataatcaacg
     2221 attagctttt ggttttttgt tgtctttgcc ggaagctcat caatcatatt tttcgaaatt
     2281 gagctttgac gaaaatgctt taagtgataa ttatttgatc ggaaaacgct aaaagcctta
     2341 tttaaatcta aagctaattt agctgtctta tctatgatgt tttgactgat ttcagcatga
     2401 ttttcagtat taattttcat catcaactga agcctttctg caatttccaa ttttttcttg
     2461 tttatcagtt gttcgggatt ttttaaatct ttttcggata aatagatgtt tagattgtca
     2521 tagatttcac tgcttattga tttgatcatc ataagcaaat gcgataaatc agaacccatg
     2581 ctctccatca ggtcttgatg taatgcgttt agcttttgct gcgtaaatct cactaatatt
     2641 ttttgatagg ctagtaggtg ttctgattct ttattggatg aaaggagttt taaaaatttg
     2701 attttggatt ttatatattt ttcactcaac ggatcatcaa cataagatcg taaatcaaat
     2761 ttatttaatg gaattctaat ataggaaggt gaactaatga tgattttatc tatctcttcc
     2821 ctataattgg ttatactctc ttgcaatgtt ttttcaatga tgataagagt ttcatctcgg
     2881 taatcattta aaattctgcg aagtcgaaac caaaggttgg cattatattt tgaaaacaaa
     2941 atgttgatat tcccatcgtt ggtatcttct atttttttca acagacctga aaagtgcttt
     3001 tcaattgatt tggctacatt atccaatagg ctgttctgct gatcgaatag ccggttataa
     3061 tactcactat tctgatgctt cgcaaggttt agtaacttct catgaagtga ttttaattca
     3121 atggatagtt caattttccc gggaatttcc agaaattcag tttgaataac cggttcgaaa
     3181 gttttttctt cggtgtaggg tagagtttca ctttggctga tctgtatgtt ctttgccccg
     3241 atattaagct ccttaaaggt ggtagaagct tttaatagga ttaccgttcc gatatcctga
     3301 caaagagcat tggtctcttc tacaaattct ttttccttac cggaaacaat atttggtaat
     3361 ccgagaaata tcaggtccga gtgtgtcgat tcagcttgaa cgatttcata aaatgaacgt
     3421 ttttcgattt gattatttat tactttaatc tccgccttta tgcgcatgtt gtcgaggata
     3481 tcttctgtct tccaataaat tatatgtttt tgatcgttta ttggatttac aataagaatt
     3541 cgtgttttgg ccttaagcca tttatctgat gattttagaa atttttgcag tactaagcca
     3601 agattgccat tatgttctgt tgttcgccac caaatatcta tctgtttgta attgccgaat
     3661 cccacttttt tgtcataatc catcaaaagt acgttcaggt cgagggctga gatgtagttt
     3721 atcgtctgaa caaatttttc aggatccttg ctttgcctac cccagccaag tattacggtg
     3781 ttaggctcta ttccggaaaa accataaaca cttgaaatgg tgcgaatccc ttcgtaaaga
     3841 tcgctacaga agtgctgcct tgagaagaag ccttttattg aatccttatt gtcgaaaggt
     3901 aaactttgtt tgtgccgtgg ttttataggg tcgcttattt ctgtttttat cagatcgaag
     3961 tttgaaagca gcccttggtt gccgattaat tgttttccga attcaatcaa atgaggccta
     4021 ctctctgaat gcccgctgaa taggatgatg ttgggttgcc agttgcgttc ttcaattcct
     4081 tttcgagcta tttgtatcag cgatgatctt acaactgacg accatacgct ttgccacaca
     4141 tcgccataat cgagtttaag gtcttttttt gagagaaaaa aatacaaaag aaacatcaac
     4201 aatatggcga tagtcattgc taatgcatca agcttgaaca ttattgcaaa gcttgcaata
     4261 aagccgatga taccaatcca tccgggtatt ctgaagctcg gcctgaaatc agggcttgcc
     4321 cagttttcga gaacatagct gagattgata aacccataag caaccatgta aaacattgat
     4381 actacacgag ccacaacgtt aaggtctcca attaaaattg caatttgtgc aattatgaaa
     4441 ctgaggataa gggcattgcg cggctcgttg ttcactccta cgcctttgcc aaagactttg
     4501 ggcattaatt tatctcgtgc tatcgcttgc ataactcgtg gagcgcctaa aagattacca
     4561 agccctgatg aaagagtagc agcccagacc cctgcaataa ctaatggcgc ccaaaaagca
     4621 atattggata aaaagttgta atcattaaga agaatttgcc tgtcaacaaa acaaccaaac
     4681 attacagcaa gactgagata gataaccata cttactaaaa ttgcaagcat tgttcctttt
     4741 gggattgact tttttgggtc tttcaaatct cccgacattg ccacaccaac agtaaaacca
     4801 gtaactgccg gaaaaaatat agcaaatatt gcctcaaaag gaagcccgtc ggggcgtgcg
     4861 ccaaaagcta tatgatcagg ccgtacatcc accctaaata tcaaacttaa aacaatggct
     4921 ataagtgata tgatgatagt tgttagaatt acaaattgag accttaaagc gagcgatgta
     4981 ctgataaaaa caataattaa aaggataagt attgccgcag tacctactat tcgaatatca
     5041 tttaatgttg ttcctaatcc aaaaaaagta cgtatccact ctaccccgag gaaattttca
     5101 gcaaaaccaa taagataaag ggcaatactt aatgacatac caataaaaag ggtaatgccg
     5161 atagcgcccc ccatcggtag cccaagactg cgggaaagca tataataaat accaccggtt
     5221 ttaatctttt tatcggtagc tattgatgaa atgctcaaac ctgttgtgat agaaataaca
     5281 tgtgccaata aaataatggc taatgctttg aatattccgg cctcccctac aatccaaccc
     5341 agacgaaggt acatgataac tccaatgata gataaaaatg atggagtaaa tacgccggta
     5401 aacgttccaa actttttggc ctgtgacatg ctaaattatt tttttgttgg taaaagtatg
     5461 aaaaaaattg ttaaacgctg tacaaaattg ttcctgattt taacttaaca gcgaccaaaa
     5521 taaccatcat attgtgataa aagattaatt tgagttgttt tttttgacta atgtttttgc
     5581 cgaaataaat aaccaaaact cttattttgt ttagagttat caaacaatat tgcgataata
     5641 ataaggattt gcagttttat tcgaaaggta acagaagcat ctaattgtgt tgtaatagtc
     5701 tattaatatt atttttgctt gttttagaat cattaagact actttgaaac attgaaaaat
     5761 gcttattgga caccctgata ggaaatttaa aaaaaaaacg caatttctat acgagctttc
     5821 ggaataatta taattccgat tatgtcaaat gaaataaggg ttttatataa aaagtaaaac
     5881 ataaaccatc atgaaaaaaa ctttttctct tctttttgtc attattctct acgcaagtac
     5941 agttttttct caatctcttc cacgtatgtt tcatttttcg gatgacagct tacgattgat
     6001 cagaggaggc gttgaatcta caggacttta tgatgaatat gtaattgata caatttggct
     6061 tagttttagt cagcccaact attggcaatt gttaacttcc aattataact cgcagaccaa
     6121 catccctgcc acgcttgttt acaagggcgc aacttttgat agcgttggcg ttaggtttag
     6181 agggatgacc tcttactttt ttgtgcaagg tcaaaagaaa tcctttaaca ttgaacttga
     6241 ttgggttcat aacgatcaac acatcgaggg ctacgagacc ctcaaacttc ttaatagtta
     6301 cggggatcct tcgatattac gggaagctct ttatctgaaa tacagccgtg aacatattgc
     6361 cagtgcaaga ggtaatcatg ttcagttatt tatcaatgac cagaactggg gtatttacgt
     6421 caatgtacaa aagtttgacc ggaaacatgt gaaagaatgg tttgttgacg ctgatgcaac
     6481 acgctggaga gccgaaaaag tgggcggtgg tggcggtttt ggagccggta agagctcgtt
     6541 aaattggctc ggctgggata ctacgaccta caagcaatat taccaaataa aaaaatatta
     6601 taaacctaat ccgtgggacg accttgtaaa tacatgttac gtgttgaata atacaccaag
     6661 taatctgtta atggattcat tggctaatta tttagatatt gacggatcgc tgtggtttct
     6721 tgcacacgaa attcttttta ctgacgacga cagttacatt gaaaaggggg gtatggatta
     6781 ttacctttat ttcgatgtag gctctaaccg aatgatacca attgaatatg atggcaatag
     6841 ctgcatgagt gttcagaaag cctcacaatg gtctccattt tataaagaaa acaatacagc
     6901 gtatcctctg atgaacaagc tgatggctgt gcccgaaatc cgacagcgct atttagcaca
     6961 tatcagaacg ttaatggaaa caacatttga cttgcccgtt attcagggat tgattgacga
     7021 ttgggctgca agaataagta caaatgtgca gaatgacccg aagaaaattt attcatttac
     7081 gcagtttcaa tcggaattat caaagttgaa acagttttgc tcaacacgac gtaatttcat
     7141 attaaataat accgaagtta accgcacagg gcctgcaatt tccaatgtca tttatagtgt
     7201 tgaggggcag aactttatta aaccagaccc atttcaaccg gtggatatca ccgcaaacgt
     7261 tactgcaaca cagggagtgc ataaggtgat tttatattat ggaactggtt tgatagggaa
     7321 gtttactcgt gttgagatgt tcgatgacgg taaccacaac gatggtgttg caggtgatgg
     7381 tatttatgga gcacagatac ccgctcatgc atccaaccaa tgggttaggt tttatattga
     7441 agcattagct aatgatacat ggaaaacggc aaaatatgaa cctcaaggcg ctgaacacga
     7501 tgtatatatt tatcaggtta aacctggtca gatcttagat agccccgtca ggatcaacga
     7561 gcttatgtcg aacaatgttt ctgctgttac cgatcctgcc ggtgaatatg atgattgggt
     7621 agagctgttt aacactactg ccgagccggt agatctgagc ggatatcatc tttccgataa
     7681 acagaaccag ctcgatttgt ggcaatttcc acagggaacg atcattaatg gaaatagcta
     7741 tctgatagtg tggtgcgata atgatttagg gcaggagggg ttacatgcca attttgctct
     7801 ctccaaagat ggagaacagc tttacctctc aactcccaat cttttagtag ccgatgaagt
     7861 ggtatttggt ccctctgatc ccgatatggc ttatgcccgc gtacctaacg gtacaggaaa
     7921 ttttgtttgg caagaaccta ctttcaatgg acctaatgat cttccattct ttagcccaat
     7981 ggtgattaac gaactaatgc ctaacaatac aaattcgatt caagatcccg atggagagta
     8041 cgacgactgg attgagtttt ataatttctc gtccgacaca tgcgatttaa gcggttatta
     8101 tctttctgat aatgcaaatc agctaaacaa atggcagttc cctgccggta ccaccgtaca
     8161 aggtcatgga tatcttatag tatggtgcga caacgatctg acccagcaag gattacatac
     8221 taatttcagc ctgaacaata gtagcggtgc aatttatttg tcaggccctg atatggaacc
     8281 ggttgatgag gtggtctatg cttcaggtag tcctgatata gcttatgcaa gagtgcctaa
     8341 tggggtaggg gattttgttt ggcaacagca tacctttaat gatcataatg gcccgtttat
     8401 cgaatgcgat gttgtagtta atgaattaat ggctgataat gtttcagcag tgcaagatcc
     8461 tgatgacgaa tacgacgatt gggtagagct ttataataat tcatctcagc catttgatct
     8521 tagcgggttt tatctttctg ataagataaa cgaacccaat cgttgggaat ttcctgaagg
     8581 aacggtaatt gaaggtaatg gttatctgat tgtgtggtgc gacaatgatt tgaatcagga
     8641 ggggttacat gccaacttct cattatccaa aaatggtgaa aatgtgattt tagcatcgcc
     8701 ttatattcaa attattgatc aggttaccta tggctttgca ccgactgaca cggcttatgc
     8761 ccgcgtgcct aatggtgtcg gcaactttat ttggcagcat cacactttca acggacctaa
     8821 caacatcgaa tttgactgtc aggtggtaat taacgaattg atgccggata atacgcaatc
     8881 agtgcaggac ccaaacggtg agtacgacga ctggattgag ttttacaata actcgacaga
     8941 gccttgtgat ttgagtggat gctatcttac cgatgattat tatgtgttga ataaatggca
     9001 attccctgaa ggaacggtaa ttgaaggtaa tggttatctg attgtttggt gcgataatga
     9061 tttggggcag gagggattac atgctaattt tactctggac aataacagtg gcgctcttta
     9121 tttttctgac ccattccaac aaatagttga cgaggtagtt tatgagggag caagtcctga
     9181 ttctgcttat gcgcgtcttc ctaacggtgt tggagaattt gtttggaaag cacatacctt
     9241 caatttaaca aatgattatg gaataaatgt tgaagagaga caagcttttg atgggctatt
     9301 tgccatattc ccgaatccgg ctacaaaaaa attaacagtt atagtaaatg atgatgatcc
     9361 cgtattgata acaatctata atttgcttgg cgagaaagtg cttcaagtat gggttgatca
     9421 tcagatggag ataaatactg ccgaatttca ccggggaata tattttgtgc ataaacaatt
     9481 tggtggcatg gaaaaattga ttttgcgtta agacgaaatt taaaggtatt gatattcatt
     9541 aagctgacat tataaattag attgtatcac tgtatcttcg aatgccaagc gccactctat
     9601 ctcctgcaaa atcttaatat gcttaccaca attgattaat cctacttatg cttaaacaca
     9661 attcagctaa taagttcagg aagcggtctg gtaaatcctt ttctatgatt gccaaaacaa
     9721 tggttggcct tgaagagacg ctttttaatg agattgttgc tgctggtggg gaaaaagtta
     9781 ggttgctcaa tcgtgctata tcttttaatg gagatacagc ctgcatgtac cgagtcaatt
     9841 atacttgccg tacagcttta cgtgttttga aaatcattgc tgaatttagt gttaacaatg
     9901 aagatgagct ttataatgaa atctttaaag ttaattggtc cgactacctc aatgttgagc
     9961 aaacttttgc agtagatgca atattgagta aagcctccat aacaaattcc cattatgcag
    10021 ctcaaaaggt aaaagacgct gttgttgacc agtttcgtaa aaaagagggt aatagacctt
    10081 cagtaaggat atcgaatcct gatgttttga tcaatttaca tcttactggc aattggtgta
    10141 cactatctct tgacagctct ggcgaatcgt tgcataagag aggttatcgt aaatggcagg
    10201 gcgatacgcc gattagtgaa ttattggctg ccggaatgat aatgattgcc ggctggaatg
    10261 gtcaaaaccc attttacgat cctatgtgtg gctctggtac attgctaatc gaagctgcaa
    10321 tgatagccgc taagatccct tcaggatacc atcgtaaaag ttatgggttt atgaattggc
    10381 ttgattttga taatgataca tggactaaag tgaaagatga agcgaatggt aaaatagtcg
    10441 aaataaaaca gcatattatt gggtcggatg taaatcagaa aacattatct ttaatgaagc
    10501 aaaacgttgc tgccgctggt tttgatgatg taattctttt aaaacaggcg gcctttgaag
    10561 atagctctcc actcgtaaac caatcaggta cgattatcac taatccaccc tatggcgagc
    10621 gccttcaaaa acgggatata aaaagctttt ataaaacgat tggtgatgtt ttaaaacaga
    10681 aatatgccgg ctgggatgca tggttgatca cgtcggatta tgaagcttta aagtcggttg
    10741 gcttacgcac atcacgcaag ataaaacttt ttaacggccc tcttgaatgt cgttttgtga
    10801 aatatgaaat gtatcaggga agtcgtaaga aagtcattcc cgaagattag acttctattg
    10861 aggctgcagc tttaccatcg taggcccatt tcaggtaaat ggcgccccat gtaaatcccc
    10921 ctccaaaagc agcaaggatc aggttatccc cttttttaag ttgtttttcc cattcccaaa
    10981 tgcaaagtgg aatagttgct gaagtggtgt tgccataccg ctctatattt atcatcactt
    11041 gttctttttg gatacccatt cggcgagccg ttgcttctat aatgcgcata ttggcttgat
    11101 gaggaaccaa ccatgcaaga tcttcagggc gaatattatg ttttgtcatc atttcaacgg
    11161 caacatccgc catttttgaa acggcaaatt taaaaacagg ttgaccttcc tgatagatat
    11221 agtgttcacg ggcatctact gtttcgtggg aagctggctt tactgaacca ccggcttttt
    11281 gatgaagatg gatcctccct gatccatccg attggtgtat agagtcaatt atgccaacat
    11341 cctctgttgt cggttctaaa agtatagcgc cggcggcatc gccaaaaagt acgcaggtgg
    11401 ttcgatcggt atagtcaacg atagaagaca ttttgtcggc gccaaccatg attacttttt
    11461 tacacatacc tgtctctatg tacatagctg ctgtggtaag cgaatagatc tgccccgaac
    11521 aggcggcatt gatatcgaaa tgaaaagcgt tttttatacc aaccttgtcg gcaataatgt
    11581 ttgctgttgc aggaaactgc atgtccggtg ttacggttga gcaaatcatc aaatcaattt
    11641 catccggatt ggtgttggtt ttctttagca gtccttttac tgcttcgact cccatatctg
    11701 aggttccttt tccttctcct tttaatatat gtctggtttt aatgccggta cgtgtcataa
    11761 tccactcttc attggtatct accatagtgg caagttcatc gttagtgagc acgtacggtg
    11821 gtacataaca atgtatccct gtaatagcgg cttttatttt agtcataatc tattgaaatg
    11881 ctgttaattg gaatatttgt aaagcgcctc tttgacttta tcagaaagtc tggttgtggc
    11941 gatattatat gagagcttaa gcatgttttt aattgctata gcgcttgata taccgtgtcc
    12001 gagtacaacg gtaccgttaa gtccaagtat tgggctgccg ccaaagtttt cgtaattgaa
    12061 ccgctcgaaa tagtcatcga gcaggttgcg cttttgcatg atgcgataaa aggcttcaat
    12121 ttgctttagc aggatgtttc cggtaaagcc atcacaaaca atgacatctg ccttgttatt
    12181 gaataggtca cggctttcga tattacctac aaaaaagtac tccttcgatt catgcatcat
    12241 ctcataagcc gactgggtaa gcaggttccc tttgcccttt tctacgccaa tattaagtag
    12301 gctcactcga ggcttttcga tccccataac ctcttgtgca taaataaaac ccagatgcgc
    12361 aaactgcgaa agtacgtcag ctttggtgtc agtatttgta ccgatatcaa gtaagatgga
    12421 agtatttcca ttttcctgag ggatatgtgc aaaggttgtc ggtctgatga cacccttgat
    12481 tggtcctacg ctatacattg acccaactaa catggcgcct gagttgccag cgcttgaaaa
    12541 cgcatcaatc tcgccctttc gaagcatacg caatccaatg acaatactgg aattttgctt
    12601 tgtcgaaata gctttcagtg gtttttcacc cgattctatt acttccgatg aataaactgt
    12661 ttcaatgcgc ccgggaggaa tatttaaatc ggatagttta gtagtgagaa aggcttcatc
    12721 gcctattagc acgagttgta catcatgcgg cagctcgtca agggatagaa gtgcaccctt
    12781 tatggtaact tcaggggcaa aatcaccacc catcacatcc aggccaattc tcatttagtt
    12841 actgattatt aaaaacacta aattggataa tcaattatgc ttcagtcttt tgaatgagta
    12901 acttgccttt gtaataaaga tcgccatcaa cccagtatgc tctgtgataa atgtgtacgg
    12961 tacctgttac cggacacttt gccaaggtcg gcatggtagc tttgtcatgt gttcttcttt
    13021 tatccctcct tgctttggag actttgcgtt ttggatgagc catttgttaa agtttatata
    13081 atgttattaa aattcagtta tttgtcattt agttttttaa gtatctccca tcggggatca
    13141 acttcacggt tttttgaatg ttggcttaaa agtatcaaca tctctttgtt gcatgtacta
    13201 ttcccgtttt catcgtcggg gtggatacat tgcattggta acgacagact gatgaattca
    13261 taaaggtaat gtgccatatt aagttgatgt tccgtctcag ggattatgat aatttcatca
    13321 gatacatctt cccattgatc gccaaatttg aaaatcaatt tgtggacgac attcacagga
    13381 taattgatca cgtcgaggca acgatcgcac tccttttcaa catacccttt gatgtcaatt
    13441 ttaaaaacta acatcctcgg ctgtctgtcc aactcaagat tgactttcag ttcccctttt
    13501 ttgaactccg aatagtctat tgcttcaaag aactttgatc caatttcgta gttaaaatga
    13561 tgaatgccaa cctttaaact tgcaaattca atattatatt ctctaaggta atccatcaca
    13621 aacccttaaa aaaattgggc tgcaaaatta gaaatatatc tttatataaa aagtatcttt
    13681 agatgtcatc tttttttata ctatcaataa ttttgtaaat agttaattat gaaataattg
    13741 aaagtaaata aacctcgaaa ttaacccttt aacaggacga gtttatttgt ttgtttgaat
    13801 tttgtgagta gaaaaagtta caaaatcgta acctgctatt aagcttcatt tatcaaatgt
    13861 aattgtagta tttatcctaa tgcatcatcg tttcgcttta taaaatgtct ttttcggatt
    13921 taaaatgaat gtatttaaaa gcttacgaat gcttttacaa gatgtcgcta aaccggaact
    13981 gtataacttt gtttttttaa aaagttgttt cggctatctc atttttatga gatgcaggtt
    14041 ttagtttgat caaagcatac attgaaatgg aactttttta tagacattat ggcaacgggc
    14101 agccattcat tgttttgcat ggcgtttttg gggtttctga taattgggtt tcttttgcca
    14161 ggagaatggc ttctgacttt gaaattttta ttctcgatca acgcaatcat ggtcgttcgc
    14221 cacatcatcc tgtacataat tattttgcca tgtgctatga tctgaaggag ttcattgaca
    14281 ggcaacagat aaggaatccg ataatattag gtcactctat gggaggtaag gtggcaatgc
    14341 tttttgctct taaaaatccg gaaataccaa aagctttgat catcgctgat gtgagcttaa
    14401 gaagttacca caaacaacaa ctacacctta gcatgataga tgccatgatg agtgttgatt
    14461 ttgataaagc agagaccaga gatgatattg cccgacagtt gtctgttgcg ataaaaaacc
    14521 agcgaatttt gcagttcgtt atgaaaaatc tttacagacg tcaaagtggc cagtttgcat
    14581 ggaagcctaa tttagaagct ataaactcga atatggatga gattctcgat ggaatagaat
    14641 cgaaaaatgt ttttactaaa cctgcgctat ttattcgtgg cggccaatcc gattacatca
    14701 cttatgatga ttttgatcag atttatcgca atttctctaa agcggagatc gaaaccattg
    14761 agaatgccgg acattggctt catgcagatc aaccggaaga atttgaaaaa aagataaggc
    14821 gctttttaac ccaaactgat caatagaaag cttttttggg gcgaactatt gattttatga
    14881 agataactaa gatttagtat ttgttcaata caatttttat tctgctgata attagcactt
    14941 aattgtttaa tgttctcaaa aactaaattt gagtcaaatt gacatcaact atctttaaaa
    15001 taaagataaa atcagtagtt tgtgtaagat agttgtggag aggtgtctaa cttaactggc
    15061 caaattgttg aagaatacag ttaaaaaata ctttttgctt gactattctt ctttttctat
    15121 ttccaacaat ttttcttgct ttatatcgct ttcaacttct ttagcagccc tattttcgca
    15181 aaatttgcaa agctcggtga tatttttcaa ttcccttgct tgggcaacag taccctgaaa
    15241 aatctctgtt gttacatcct tattaatggc atggcaatcc tcgtaaatat gatattttgt
    15301 tcccgacttt gtccaataga cttcgttttt tccagttaac tcttcaactc tttgagtttg
    15361 ttcagtatat tgttcaactg atggcggatt aaaatctatt cctgtaactc cggctattat
    15421 caaagctaaa ccagcaatgc caccaaggat tcccttttgt ttagcgtcaa ggtttttatt
    15481 tgtaagtata aaaataacaa gcggtaaaaa tgcgataatg gcaactacca accctaactg
    15541 gctctgcata aaaaacagaa atttgttttt ctctgatgca gggtctaacc gatttgactt
    15601 tttccataaa atggaacctg tgattgccaa tgctaaatct attactatca gaattattat
    15661 ccacatcata ttgatgggtt gcctaaacaa tagggagatc gctacaacct gtgcgccaat
    15721 ggccaatagc caaagtatag cagatattac acgcaaccga gtggctttgc ttttgctttc
    15781 ggcagtgggt ttaaaggtct cggtagttcc gatattcgat gagttgttac ctccaacgtg
    15841 aacgattttt ttctttgcca tatcattaaa tttaagttaa tattagaaat ttactcataa
    15901 gtctttccgg tagtctcttt gtttttttat gaatgttgtc cggaattaat aattgctgac
    15961 aataaattta tacaaacata caaaagtaga tttattttct ttacatcttg tagtaaatag
    16021 attttaaaac cttgttaatg aaaaatcaaa acccatccct tgaatttaag agtagagcga
    16081 gtgatctgtt attctttttt ttataagtta ttattacgac ttgaagtcca atcttcgtgg
    16141 aacttcattt ttgtctattt ttacactcaa aaatttgcct ttaatgagcg aatcatatat
    16201 tctacatcag cttaaaaagt ttagaaatca aattgagggt gatttactaa aagactactc
    16261 aagccgaatc ctttatgcta ctgatgcatc agcatatagg gaattgccac tggcagtaat
    16321 aaaaccccga aacaaagagg atatccgtaa attagtgctt ttcgcaggta agcatggagt
    16381 ctccctgata ccgcgtggcg ccggtacatc tcttgccggt caggtagtgg gtaatggtat
    16441 tgtggtagat gttacaagat atctgaacaa aattatagaa atcaacccgg aggagaggtg
    16501 ggtaagggtt gaacctggtg ttgttctcga cgagttgaat attgcattgg ctcgccatga
    16561 cctctttttc ggacctgaaa ccagctcttc gaatcgttgt gtaattggtg gaatggttgg
    16621 taataattct tgcggtgccc attctcttat ttatggaagc acccgcgatc atacactcga
    16681 aatagaagct attctgagcg atggctctat ggtaaaattt aaagccttat caaaggagga
    16741 atttcaacaa aaacaaattg gcgaaacact tgaaaacaaa ctttatcgaa atattaccga
    16801 tatcttgtct ttgccgctaa acaaagaaga aattcgtaag gagtatcccg accctatggt
    16861 gaagcggcga aatacgggct atgcaattga tttattactt gagtcggagc cgtttagtga
    16921 atcagatgtt cgttttaatt tttgtaagct tttagcagga tcagaaggaa cgcttgcctt
    16981 tattaccgag ataaaactga accttgttcc gttgccgcca aaggtaaaag gattgatatg
    17041 tgttcatttt aattcgctcg aagaggcatt tcaggctaac cttatcgcac taaaatacat
    17101 gcctggcgct gttgagctta tggataatgt tattttacaa caaaccaaaa ataaccttga
    17161 acaggcgcgt aacagatttt ttgttaaggg cgacccagcc gctattttaa ttattgaatg
    17221 ggcacgggaa actaaagaag agattgacga tgttgctgag aagatggaat tggatatgcg
    17281 tggagcgggg tttggatatc attttcctcg aatattcggt tcggatatat caagggtatg
    17341 ggcattgcgt aaagcagggt tgggggtgtt gtcaaatatt gcgggcgatg caaagccggt
    17401 gtcattggtt gaagatactg cggtaaatcc gaaagtatta ccaaattatc ttgcagaatt
    17461 tcaacaactt cttgatagat atggtttaaa agcggtttat catgcccata ttgcaacagg
    17521 cgagttgcac cttcgaccgg tacttaatct aaaagatcca aaagatgtcg aactttttca
    17581 tactatagca tttgaaacgg caaaattggt aaagaaatat cgcggatcgc tcagtggtga
    17641 acatggcgat ggcaggctgc gcggagagtt tatccctctg atgatcggac aacacaacta
    17701 caacctccta aaagagatta aacaaacctg ggatccgaac aacattttta accctggaaa
    17761 gattattgat accccctcga tgaagacgaa tttacgttac gatcccggga agaagactcg
    17821 taaaatcagg acctatttta acttttccga tgaccaaggg gtgatgcgag cagtggaaaa
    17881 atgtaacggg tcgggcgatt gccgcaaaac agaaattatt ggcggtacaa tgtgtccgag
    17941 ttttatggct acgcgaaatg aaaaaaattc aacaagggca cgagccaata ttttacgtga
    18001 atttctgaca ctctcgccaa agaaaaatcc tttcgatcat agcgaaattt atgatataat
    18061 ggatttatgc gtatcgtgta aaggatgtaa atctgagtgt ccgtcaagtg tggatatggc
    18121 aagatataaa gctgaatttt tacaacattg gtgggataag catggaatac ctttgcgaac
    18181 acgtgcagta gcatatatat catctcttaa caggatcgga atgctctttc ctgctattac
    18241 caattatttg tttactaata aattcattgc caacttattt aaaagggtat tgggaattgc
    18301 tccaaaacga tccattccat tgctataccg atatacacta tctcggtggc ttaaaaggaa
    18361 caaacataaa ttattgcctc accggaaacc aataggaagt gtttacctct ttattgatga
    18421 gtttaccgag tataatgata ccgaaatcgg tattaaagca gtggagttgc tcacacggct
    18481 tggctatgaa gtaaaaatag ctgttcataa agagagtggc agggcattta tttcgaaagg
    18541 actgataaga aaggccgccc gacttgccaa tttcaacgta acaactttta aaaatatcat
    18601 tgatgctcaa actccgttag ttggtatcga gccgtcagga atactctctt tcagagatga
    18661 gtatccggat ttggttgatc cagaacttag gccggaggca aaattattag cccaaaatgc
    18721 gcttatgttc gatgagtttt tcgaaaaaga gataaaagcc ggaaaaatat cccgcgacct
    18781 gtttacaatg gaacatcgaa aaatcaaact gcacggtcac tgtcatcaaa agtcgctcgc
    18841 ctcaattgaa cctacgaaag cgatgctttc tttacctgaa aattttcagg tagaggagat
    18901 taaatcggga tgttgcggca tggctggttc gtttggctac gaaactgagc attatgaact
    18961 ttcaatgcag attggcgagt tagtactgtt tccggaggtg cgcaaagcgt caacagaggt
    19021 gattattgct gctccgggta catcgtgccg acatcagata aaggatggta ctggccgaac
    19081 ggcttatcat ccgatcgaaa taatgtatca ggcgctcctt aaaaaataga aatggaataa
    19141 ctaagagcta ttttaacgat tgatcatgaa ctgtaaacga cttgcaagac tactaataaa
    19201 aaaaactccc cgaagaacga ggagcttttg caagtagtaa gttattaatt aaaacgtgca
    19261 atatgcacct aaggtaaacg atcactttta aataattatt ttatgcaaca ggatcaggca
    19321 ttacaaataa ctgtaaaaga ggcgtcgccg gaagatcagg aaatgataat cggttttcag
    19381 attgacatgg cttgggaaac tgaaaaggta aagcttgatg agaataccat aagacttggt
    19441 gtgcaaaatg ttttcaaaaa tccaaatctt ggacggtatt atgttgcaaa agcaaataat
    19501 cttattgtag cctccctttt gatcacttat gaatggagcg attggcgtaa ctctattgta
    19561 ttgtggatac aatcagtata tgtaatccca aaatacagga aaagaggtgt gtttaggaaa
    19621 atgtatgaaa atatcaaaca acaggttgaa agagataatg aaataaccgg tatcagacta
    19681 tatatggtac ataacaatag aattgcagat catgtttatg aaaagttggg catggatggt
    19741 aatcgttacc gcttgtttga gtggttaaaa aaataataat ctaaccacaa agaacgcaaa
    19801 ggttttacac aaagaacgca aagaaaaaac tctgcgacct aagcgccttc tttgcgaact
    19861 ctgcggttaa aaaacaataa tgaaaccgca aagaacgcaa aggttttacg caaagaacgc
    19921 aaaggaaatt ctgcgaccta agcgccttct ttgcgaactc tgcggttaaa aaacaataat
    19981 gaaaccgcaa agaacgcaaa gaaaaaactc tgcgacctta gcgcatcctt tgtgaactct
    20041 gcggtagaaa aataattcac tatgattgaa aacgaattga cgaataaata gttgatacga
    20101 gatacaattt ttatgatata aatattggag atcaagtatt aattatttca actacttaac
    20161 agaatagccg ctatctatgt tggtagatga taaaaacgag cgaaaagacc aaacagttaa
    20221 ttctccaact tgatgaaata cgttttactg acgtgtatgg aatacatgtc taattatttt
    20281 tgaaatgaca aacttatgtt atttgtttct tgcgaatatt gtatatttgc ttcgtaaatt
    20341 agacgttggc agaaatatta aaaagacacc ttgtaaatgg aagagaaata taaacaatac
    20401 gaaaagacaa ttaaaaagaa gcatagtttc gcttggacac cgaaatatgc ttcagaattt
    20461 agcacaagac taagtgctaa ggaatttatt gcaatcgcag aaaagacatt taaagattta
    20521 ggttgggaca tagtttatca agacgacaaa tcaattgaag caaaaagaag cgaaaagtca
    20581 ttagggacag agcgttggac agaaggaatt acagcaactt ttgattatgg aaaaattcaa
    20641 gtaaaaagcg tgactcttgg aaatgaaatg tgggatgttg gtagaaattc aaaacgtgtc
    20701 caattattca tttacgcatt tcaagaaata gaaaaatcct ttgatagaga agaacttcaa
    20761 aaccttgtaa atgaaaaaga aactgtaaat aattgggacg attatgtagt tcctgattct
    20821 ttacccaaac ctaaagaaat tagagaacca aaattttaca ttcatattgt tggatgttca
    20881 atactttcaa tcttaattgc ttttttactt gcaaaggttt ccataagtgg attttacatt
    20941 attggacttt ttgagttttt agtcggattt gcaataggtt ttacttttaa gtatttaatt
    21001 aagttttcaa acttcactaa ttacgacaaa ctccattatg taatattagg gacaattggt
    21061 ttgacatatt tattaaatca atactttcaa tacgagataa tattatatga gaacaattat
    21121 gaccgaattg gtttttttga gtttctaaaa atcaaatttg aacaaggact tacaatcaaa
    21181 acactaaata cagggtggat tggcttagta attcattgga ttttgcaact tgttataacc
    21241 tactatgtga cagtaatggc agttataaga aatttaacga aataccaact tgatagagtt
    21301 caaccagaag ttgttgactt tgcgttttac cactttgtaa aggacaaaac cgaagaccaa
    21361 ataagaaagg aattagcatt aaagggttgg acggaaacgc aaaatcaaga cgaagttttt
    21421 gaagctatcg gagctttaca aacaactcaa gacgtaaata gaatgcaatg aaaagaaata
    21481 cttctgccaa cagcaaggct ttgcacaatg gcggggttga aacagatatt atagtttgcg
    21541 gtttttatgt tactttgtcg tggcttgaca gagtggtttt caaaaatccg ccattgcgcc
    21601 aagcgccaaa ccgttagcaa ccgcaagtgg cgacagctta acttgtaaaa tccgaggaaa
    21661 gaaaatcttc acagaaactt aacacctttt ctctcttagt tgtaagtaag atgaatattt
    21721 ttgtatttat ctgaatttta gatgtgtgaa aacgaactca gcttttttag taatcttttc
    21781 gattgcgatt ttttatatct gttggggagt ttctcaactg ataagcatca aaacgcggta
    21841 ctcacaacta tcaagtttac tattttccat tgtttttaca ggcataatcg gatgttttat
    21901 tccaatatat tttaaaaaca gatttcgttg gagttacaac aaacaaagtt caaacaaaat
    21961 aataggttat cttttcttgg ttttagccat tttgttgtca acaatattat caggagcttt
    22021 tgttaaaatc attgaattaa aatattcttg gggtttaatt ctgaaatata tacttctgtt
    22081 tttcccgatg tctcttggaa tcggcttgtt tgcattttta ttaattccga acacgataaa
    22141 cgattggaaa aagaacaata taaaaagtgt attgttagtt atttcaataa gcatcttttt
    22201 ctttttctca ttttacattg actcactgtt tcaagatata gagttagccg ttactatggg
    22261 atttattgga ttgctattag cgctgggtta tctatttctt agaaactttt gggttgttta
    22321 ctcagcgctt tttataataa tgttagttaa cacattagcc gacaataagt atgatgagta
    22381 cagttattgg gttgtaatta taggttcttt actctcctta acaatattga cgtttgactt
    22441 tattaaaaac aacagaataa aaatcactaa cctatcaata gaataagaaa tgacaacgga
    22501 aaaagaaaaa tcacttcaag ctcacttgga taaagtggtt gatggcaaaa aagtattcgg
    22561 gacatcattt gcttttaaaa aagatacgga aacttggcaa ggggcatcgg gtaatttaac
    22621 tacagaccgg ccttacttta ttgcaagtac cacgaaactg tttactactg caataattat
    22681 gaaactgaga gcagaaggca agttaagtct tgaagataaa ataagtaaat acattgacgc
    22741 ttcaattttg tcgggattac acttttacaa aggcaaagat tattcccaag aattaaccat
    22801 cagacacttg ctttcccaca catcgggact ttctgattat tttcaagaca aaggagcaag
    22861 cggtaagagt ttggaaaatg aattaatggc tggcaatgat cagttttgga cttttgaaga
    22921 atcaattgat aggtcaaaaa aaatgtctcc tctctttgtg cccggaacaa aaggaaaagc
    22981 aaaatattcg gatgctaatt ttcagctttt aggaaaaatc attgaaacca taacgggtaa
    23041 atcgtatgct gaaaattgtc aagaccgcat tattcagcca cttagtttaa caaaaaccta
    23101 tctctatcaa gatgcaaccg atgaaaaacc aaagatgtta tatcataaaa gcaaggagtt
    23161 gaaaattccc aaagcaatga cctctttcgg accagatggt ggaatggttt cggtttctgc
    23221 ggatatgctt gtttttattg aagcattttt cacaggcaaa ttattcccac tatcctatat
    23281 tgacgaattg caagaatgga acagaatttt tcccccaatg cgtgcaggtg ttggcattca
    23341 tttgtttaag cttccttgga tttttgatcc tacaggtgca attccatatt ttatagggca
    23401 ttcaggactt tcaggcgctt tggcatttta cagtccaaaa gaaaatatct atatcgccgg
    23461 aaccgtaaac caaacagcac accccgatat ctcttatagg acaatgattt cattaactca
    23521 acgaatattg aagaaatgaa agatagaaat aattcagtta caatttttaa tgcccgaata
    23581 atcgggattt tctttttatt ggctttttta gcctacggta tcggaagaca attgtttgaa
    23641 agtgctgata agcctgaaaa atatttaggc gcattactaa ttctcgccaa ctcggcaatg
    23701 gtcctgttta tcgggatttt attgagaaaa acattgaccc gatacaatcc aatagttggc
    23761 aatatttatt tttttacaag aatttttgaa gccttagctc tttcgacaat attattgaaa
    23821 ggtgttgtat ccgacgactt gggatatttt cttgcaatgt tggttttagg gattggcagt
    23881 attccaatgt gtttgacatt gtacaaacat aaagtctcac catcttggtt ggcgatttgg
    23941 ggggccatag gctatgctgt ttttgctttt ggctttctga tggagctatt tggaaaggag
    24001 tggagcatct attttctcgg tccaggcggg ttgtgggaag tggttttcgc aatttggtta
    24061 atcgtttgcg gaagtaaaaa tataaaacca actactcgtt aacaagtggt tgattttttg
    24121 ataataacaa tataaaaact tattaaatag gagatagaaa aatgaaaatt agtaaacgaa
    24181 tgatttatgg aattctatta acagcaatag tattcttatt agcaacctta gcgggtagaa
    24241 acatcaattt gaatattaac tttcttccct actcgtttgt aacgcacacg actttgctga
    24301 tactttccat aactttgatt tgcggtttaa aaaagcatgt gaagtataat atggcatggc
    24361 ctaagtttaa aaagacacta aagccaatac tattcgggtt cttggcagcg ataatcgtca
    24421 atacttttat ggttgctctg acaaaaggtt taggaggcga agtcgaatct cattttgcat
    24481 ttgatttgat gtccccattg caggttttcc tctttatatt tatctatgca agtgtggcag
    24541 aagaggtttt atttcggggc tttcttcaga acatattaaa gccactaaaa aataagggga
    24601 taaaattatt taaaaggcat attagcgttc cggtaatgat tggtgccgtt gcgtttagtt
    24661 tagcgcatct aattctgata acgtcgggcg cgaacacttt ttttatcgtt agaacactag
    24721 tttttacttt tgttcttgga ttaattgccg gttattatca agaaaaatat gataacaatg
    24781 cgtatgcaat ttttgtacac atggcaggta atttcatggg tgtagtagcg gcaattttaa
    24841 cgagtttaag tgtctgattt aaacttaaac acaacaaact aaatttgaaa tgaaaagaat
    24901 actatcggta ttattagtgt tgctaatttt tacaagttgt acagatatga aaagcgtttt
    24961 tatcaaagat aaatccgaac tgatagaaag gaataaagct cataagattg aagtttttac
    25021 cgaagaatta ataaaagatc tacctgaacc tttgaaaaag taccttcgga tttgcgggta
    25081 tgtaaatacg cctgttccta taaatgccaa tgtgtattgg gctgagtctt ggttaaaaat
    25141 gtcgccggaa aaagagtgga gaaaacttca tacaacgcaa ttcaactctg taaagccaat
    25201 aggaaggtca gcctatatga aaattttagg tatgcctgtg gcggctagag atctatatcg
    25261 tgatgggtat ggggagatga acgtaaagct actgaatttg ataaaagtgg ctttcgataa
    25321 cagtaaagaa gtggcacaat cggcattgat aacgacattc tgcgagttta tgcttattcc
    25381 gggctatctg cttttggata atgtaaaatg ggaacagata gacgattatt cgattcgggg
    25441 aacagttgtg gataatggga ttgaagtttc agggatattt tactttaatg aagaggggct
    25501 ctttacacat tttgaaacat ttgaccgata ttttacgaca ggtaaaaaca gttacaaaaa
    25561 agtcaagttt tcggttgttg tagagagtta taaaactcaa tataaattga aaataggcga
    25621 gaaggttaag gttatgtggc atttgccgga aggtgattat gaatactata aaggtactat
    25681 tgacagaatt gagtttaatg tggttgagta ttgaagaaag ggaattgtaa agtcaaaaag
    25741 gtgtacaaat atcttaagta taatatttac gaatgttgta actttgtttc gtaaattaga
    25801 cgttagcaaa cataaaatga ctgacggtta acacagggct gaccacccat tatgaaatga
    25861 aattgagacc aatactcaat ataaaaaaag gggagaaagc tgaaaatcct ataagagtaa
    25921 gcaataattt taaattttaa tcaaaatgaa atcattaaaa atttaccaat ttaaaggaat
    25981 tgtatttatt atgttcctaa ttttttcaag caatttgttt acaggttgca aaaaagatgt
    26041 acttgtttca aatgatgact tacagcctat aatgactttt gaaaacatca aggtcaaggt
    26101 gcttgaagac aggttgtatt ttgaaaaccg agcagattat gccgaagcaa ttgaaatact
    26161 ctcaaatatt gaggaaagca cctttgattt gtttgaaaaa agcataggtt tccaatcaat
    26221 gagaacagtg ctaaatcaaa aggaaagaga agcgatcaat attgaagatg aacttttagc
    26281 aacactatta aatccgcagg ggattattca aatagcagaa aacatttttg aaattgacgc
    26341 gttgaatgaa atagtgactg tgtattcagc acctgaaaca agagaaaaaa atggtaaacg
    26401 aactttcagt acgaatgatg atgtatttga cagactagac ggcatagaag cgttatatac
    26461 aaagtcaggt tgcccatctt ataaagaaaa agttaaaaac tgggcagttt ggggtggcaa
    26521 tgtggagtgc aaggtagttt atcagaaagc tggaatttat ttttctttgc aatcaaagat
    26581 caaaaaagat cactggggag gttctgctta tattagttta acttgtccaa gtggcagttc
    26641 gtatacccaa aatcgcagtg gagcatctca gcaatcaatt ccttcgaatt cacaaggtgg
    26701 ttacggcaga gtgtataact atagacctta tagcagcatg agaggattaa aacagtatta
    26761 tttctctgta aatttttact gtgatgattc tgtttcaggt tatgggcctt attctacaac
    26821 attgacaata aattgtaatt aagttcgttt tttagggagt tggtttattc caactcccta
    26881 ttacacataa atatatgaga ttaatttgtt ttggttgtct gtttttttta atatttggtt
    26941 gcaccaaaga aataaacatt gaattaccta tgcaagattc aaaaatagta gtaaattctc
    27001 tatttactaa tgatgctcct ttatctgtat atcttacaaa aacaagaaca ccttttaacg
    27061 agaaggaatt atttattgag aatgccagag tcattattta ttcagagaat ataactgaca
    27121 ctcttcagta tatgggtgaa ggaatttaca tgtcaagtca tttagggctt gcaaattctg
    27181 aatatagaat agatgtaata tctaatggtt ttgagaaggt taccgcaaga gattatattc
    27241 ctaagcaaat ggcactgccc ttaattgtaa gtggtttccg tgacagtgtt ggcgttaatg
    27301 agcatgggga ttactattca caatttacta taacattttt ggatgatgct aatgcaaaaa
    27361 ataattacga aatttttata aaacatcttc tttttgaaga tgaagtatgg gatagaggtt
    27421 gtttttcaaa tgaccccatt atcgttgctg agggtttaaa tgattacttt cctaatacag
    27481 tagtattttc cgatgccttt attagtggaa aggaaaaaga attggcaata aactattatc
    27541 cagttacata tagtaagcca acaggagggc aaggcgaatt ggttgaccca aactataaag
    27601 taatagttta tttcagaaat acttcagaga attactataa atataaaaaa aaactggcag
    27661 ttcatttagc gggccaggaa tttgattttt gggcaggttc tggtgagcca gttcaaatgt
    27721 tttcaaatgt tgtaggtggt tatgggatat ttgcaggata taccgaaaga atagatacta
    27781 tacaaaaact aaaataatta cacatgaaga aaaacatcat tttattatta tttgcctttt
    27841 tgccgatatt ggttattgca caagaagtaa agagaaacaa aagcattgaa attattgcag
    27901 ggtatgcaaa ttataattac aatttgaatg gtgtgttttt aagtgcaggt tgctcaagag
    27961 gttttaacaa atatttatat ctaacaggaa gtttaacctt gtcaaatgga agtcgcaata
    28021 gagataattc atttgttaac gaccaatttc atgccgctgc cctacagatt ggcttaaggg
    28081 gtgtttgatt tcttaaaaat acattcaata agattatcag ggattgctgg ctattgtttt
    28141 ttagaaa
//
LOCUS       DAOOAI010000013        28011 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_2218_length_28011_cov_5.215982, whole genome shotgun sequence.
ACCESSION   DAOOAI010000013 DAOOAI010000000
VERSION     DAOOAI010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 28011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28011
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2218_length_28011_cov_5.215982"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            110..1150
                     /locus_tag="PK345_01925"
     CDS             110..1150
                     /locus_tag="PK345_01925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33813.1"
                     /translation="MKKIILYLLLILAMFKWPAFSQTKIFDESFESDLTGWNFEGNWF
                     QEPGYIFMYYHPVTYNYDFWTISPEFQVPVTGGDLIINHFVDVYQANVTDEKCEILIL
                     HNDQEDVVWEYALSNGTWGSIFGTDMLIPLDEFIGETVRVKIKSYGAKSNALWGWFIF
                     NMSLTTFFNYDVEALQLNGPASLNPGEVGDWTLSIKNLGLNPISDITIKLFSYKEYNE
                     LATEIFNQSIPAGETSLAPITWSSNLVHNTMLYAVIEHTNDQYSANNKSQSKFLRINP
                     PQEVNILVWDNDNGIETIINPETGVNQQASATIELNLQEAGLQYSLLEKLPVDLSQYD
                     IVIATMGSHCLG"
     gene            1361..1924
                     /locus_tag="PK345_01930"
     CDS             1361..1924
                     /locus_tag="PK345_01930"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33814.1"
                     /translation="MNNVDFTYAGGNEPHYTVDRFLSNGAEMLYTSEDGYHRMFAYNP
                     DSSYKVISSSVLIGALKNADSLSTKPYLLAEIVNYFLGIETATNIRELFDKKIANSVT
                     AYPNPFSSSTTIGFTLKTEGSVKADIFDVNGRHIKAFKQQVMPSGQHDFIWNGSNDAG
                     TVGQNGVYFYQINIDGKIESGKIVLNR"
     gene            2133..3542
                     /gene="selA"
                     /locus_tag="PK345_01935"
     CDS             2133..3542
                     /gene="selA"
                     /locus_tag="PK345_01935"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HPX33815.1"
                     /translation="MEERLKKIPGVDILLNKPKVKDLLNRYPRSLVRHIIQLLLKNIR
                     MEVLSGGKVPDEEEIVNDIRIKTESFSSKSLRPVINCTGIIIHTNLGRAPFGDQLLAD
                     VQHLLSGYSNLEFDLEKGERGSRYVHVTELMKFLTGAEDVLVVNNNAAAVMLILRTFA
                     KDKEAIISRGELIEIGGSFRMPDIMAASDCKMVEVGTTNKTKITDFENAILKDTSIIL
                     KAHKSNYVIKGFTTEPPLSELVDLGKKTGVMVVYDMGSGLLRKAKVKMLDDEPDVRSA
                     LATGVDLVTFSGDKLLGGPQAGIIAGRKSLIEKLRKEPMTRALRVGKTVLALLESICM
                     MYLDEKLLIDKSPLFAMMNSTIDTLEKRALLLADDLKKRDVGSKIIKSAGQAGGGSLP
                     DKTIESYAVVPQIKANSRAEKNEKAKWIYHQLLSQNTPILGALRKGELIFDVLTLSDK
                     EIKIVAEIIANTYQEANRV"
     gene            3553..5490
                     /gene="selB"
                     /locus_tag="PK345_01940"
     CDS             3553..5490
                     /gene="selB"
                     /locus_tag="PK345_01940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HPX33816.1"
                     /translation="MGTAGHVDHGKTSLIKALTGIDGDTHKEEKKRGITINLGFSHLD
                     LDDNISLGIVDVPGHKDFVKTMIAGAHGIDFVLLVIAADSGIMPQTHEHFNIIKMLGI
                     KHGLIALNKSDLVDDETIELAKLEIMEFVDNSTLEHAPIVPVSATTGFGLEALKSVIK
                     RVAEKVDTKTTTNQFRMYVDRIFNVKGIGIVVTGSVLGGEASIGEELFLLPGSHNKLK
                     IKGLERHGRQVEKIIAGDRAAINLSGLKYNDFEKGMILTKIILPEIKLLDVVLELFPG
                     NQRVGIWSQMIFHTGNFSSKARIHLLNKNEINGSETALAQIHLEKPAILLNRDRFVIR
                     NTSNDLTIGGGIIIDNRPLHHKKRTKTLSRYLETLAQATTDHENLSALILLELNKSAL
                     PLTTETIASTLNKTINEVENAILDVELQSAILVYENKNYLIPKAFIEKNNKRIIETLK
                     VWHLQNPLSESGLELREIAGKVKIMGDFNIYMLGKILEKMTFEKVIRTVGATFVLKEH
                     KVSFDRKTQEQIIWLEEIIRDSGMQRATINEVEAIALTRNIKKGQLVKLLQYLAANDK
                     IFFNGEDILHNSLVRKIRLKLLQNLSENPRGINEKEFRLLVDGTKRAIQELVNLFVQE
                     GIIEKQTFYLLITEKGKELLL"
     gene            complement(5494..6450)
                     /locus_tag="PK345_01945"
     CDS             complement(5494..6450)
                     /locus_tag="PK345_01945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HPX33817.1"
                     /translation="MIRRTPLFFHQPAVVQLVVLFLLVLCFWIVSFFLGVVLSRLFFG
                     IPVLETLSGIAIPETRNEINLLKFMQLINQFGTFLIPPFLFAFLVSTDIREYLGLQKK
                     PNLLPVLSAIILIYLLLPVINELVRINQNMSLPSCLSAVEEWMRVSEMRAEEMTKAFM
                     RTTSIKGLLINLLIIGVLAAVGEELLFRAVLIRVFRSWFKSSNWAVIISAFIFSAFHL
                     QFYGFLPRFLLGLVFGYLFVWSGSLFLPIIAHFINNASAVIISFLMNRGIIEVSVDEF
                     GKVDNPILLIISIILSIALMYFIYWYEKKADGVYLKKDNTKA"
     gene            6626..6698
                     /locus_tag="PK345_01950"
     tRNA            6626..6698
                     /locus_tag="PK345_01950"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6659..6661,aa:Thr,seq:cgt)
     gene            7210..7281
                     /locus_tag="PK345_01955"
     tRNA            7210..7281
                     /locus_tag="PK345_01955"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7243..7245,aa:Glu,seq:ttc)
     gene            complement(7398..8063)
                     /locus_tag="PK345_01960"
     CDS             complement(7398..8063)
                     /locus_tag="PK345_01960"
                     /inference="COORDINATES: protein motif:HMM:NF017857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HPX33818.1"
                     /translation="MVRSQALTGTTGYYNIPSAELYPDRSMFIGTNLLNREYKKWGRP
                     THDAMDFFITNSFLPFAEVSIRFTRMIGLDDYSSTVGDRMASVRLRVIDEGRYNPAIL
                     IGVHSFFTTLSSGAASHFNSTYLVATKNFKVNHVFENIGFTTGYGSDVLKASDYQFIG
                     FFGGVKITPYHLKPVELIVEYDADKWNVGARITILKHIVLLGGLEGFDAFSGGVSYKF
                     LLP"
     gene            complement(8191..9729)
                     /locus_tag="PK345_01965"
     CDS             complement(8191..9729)
                     /locus_tag="PK345_01965"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPX33819.1"
                     /translation="MKRRLHFVLFDVFFLTVSFLFVACLKSATVTVVLPTYSLPFVLF
                     LITWLIFSFHFKKYNSEKYINRREILYIILKSNFVSLALGLLVLYTFTIFNVSRLIYF
                     GTILTATVLEILAALIYNHVHTSKVIDYENDFKEFRSSDFPETDTLVSPPVIKKRDVS
                     DRQVEVKLILEMIESEYGKDVKDFISEYLMTVSGPVQVVSTTTRFNIFALPEKQYGCI
                     VNFHKINDFRWLNKFFETVNDKVLNEGYFIGRVETYTLRKKRIISKYLFPFNYVVYTF
                     DVIFRRVFPKLSILKRFYFWITKGKNRALSQAETFGRLYSCGFTVLDDKDINGKLYFV
                     SQKVKEPSYPSDPTYGPLVRLRRIGKGGKYFYVYKMRTMHPYSEYIQQFVYEKNRLEK
                     GGKFKDDFRVTTLGKFLRKFWLDELPMLINVLKGDIKIVGVRPLSSHYYNLYSEELKQ
                     YRIKFKPGLIPPYYADMPESFEEIMESEWKYLMAYEKHPFRTDVKYFFAALKNIIFKN
                     ARSK"
     gene            complement(9784..10848)
                     /locus_tag="PK345_01970"
     CDS             complement(9784..10848)
                     /locus_tag="PK345_01970"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HPX33820.1"
                     /translation="MEEKSNKKSSYRIVTEVSEKIVSDWNRFVQAHPEGSVLQSWYAW
                     QLFFRTKNFNPFFVACIDETDRMVGVLLAVLIREKSYIKSLFSARLVIYGGPLIESGN
                     VYDKEIFRMMLASMIDKTSRTAIFTQFRALHDLSCYTEIFKENGFQWVPRINLLINTQ
                     NKEKIFSEMSSSRKRQIRKSLNNGAKVIQPNNIGQVRQFFDILQKLYRQKVRKPLPDW
                     TFFEAFYFLIQKYNFGKYFLIEYKEKIIGGIMAPSLPAKTVFEWYVCGLDPEYKKEGI
                     YPSVLATWAAIEYAHEQGSSVFDFMGVGKPNEPYGVRDFKLRFGGSIVNYGRFIRINN
                     KLKYSIAEFGYNFLSWLKKV"
     gene            11033..11266
                     /locus_tag="PK345_01975"
     CDS             11033..11266
                     /locus_tag="PK345_01975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33821.1"
                     /translation="MKKVFSVFLFFITISVYSQQLSADGRCMPEDLLQNTILQGRNEM
                     GNFTITDSDGVTRTLYDELDSGKTILLDLFFTT"
     gene            11303..12601
                     /locus_tag="PK345_01980"
     CDS             11303..12601
                     /locus_tag="PK345_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33822.1"
                     /translation="MYVNYGQGQQNVDFWGLSPYDDNVQINNYKAQNGITYPCAGYQG
                     GAAAAINIVISGQPFLGYPTWVVICPDRSFTFDVCFPPSITCFDPYFAACGPPMAADF
                     IADQTQICETNFVQFTDQSIGNPTLWIWDFPGGNPSTSSEKNPLVEYPNAGQFDVSLT
                     ITNGIAGNTITKPNLIEVDPSPEVYAGLKGETCVNEHITVTDASSQNTSILLWQIVAG
                     QGILENSQSISPTYIPAPEDAGTTVTLKLTGNGSGECASMNVESIKEIDIWQQPEVTL
                     MPFDTICYQWDEYPLVGGYPEGGVYSGTGVIDNIFYPQIAGVGSHLITYQWVDLQTGC
                     SNSAEEVIVVVICSGIWNGTELPIFNIYPNPTKGEFVVELVQSGNYELTITNIAGIKV
                     FSAYLNESSTIKLDNVENGIYNVTIYDGKKSHTKKITIKK"
     gene            13013..13162
                     /locus_tag="PK345_01985"
                     /pseudo
     CDS             13013..13162
                     /locus_tag="PK345_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
     gene            13456..13983
                     /locus_tag="PK345_01990"
     CDS             13456..13983
                     /locus_tag="PK345_01990"
                     /inference="COORDINATES: protein motif:HMM:TIGR01752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HPX33823.1"
                     /translation="MKKIGVFYWPKGGNVESCAQRIADRFKNQFEVQIYSLDTIKGLD
                     LPYYDLVIVGGSTSGADTWQKGSGDNLWFDFFERLKNAQLHGKPVAIFGLGDQVLYPD
                     HFVDSMQIIKKEFDLIGANLVGRWPVEGYYHTNSEAIEDGKFIGLALDEDQQPELTDQ
                     RIDKWVMQIVNESGL"
     gene            complement(14046..14522)
                     /locus_tag="PK345_01995"
     CDS             complement(14046..14522)
                     /locus_tag="PK345_01995"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPX33824.1"
                     /translation="MTKKLLINQVLLVFLLVGIGFQSCKKDDIKKKQAEKDEQIIKDY
                     LATNQLEALRHESGLYYIIIEEGSGAHPTVNSTVKVLYKGYLTNGEIFDQTNQDARVF
                     KLKNLIEGWRIGIPMLKEGGSGIFLIPSAMGYGSNQVGAIPPNSVIIFEIELLSIL"
     gene            complement(14627..15646)
                     /locus_tag="PK345_02000"
     CDS             complement(14627..15646)
                     /locus_tag="PK345_02000"
                     /EC_number="2.5.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyhypusine synthase"
                     /protein_id="HPX33825.1"
                     /translation="MNKKDFLNRVVKHIDIKSFDSTPIIDSMRDMSFTSREIAQAADI
                     LQLMIKDANCTNWLTLAGSTSAGGCMQVYVDMIKNNMIDVVVATGASIVDMDFFEALG
                     YKHYIGSSWADDKQLRSLYIDRIYDTYIDEEELQACDETTKIIANSLEPRPYSSREFI
                     KEMGRYLTKHAVKKDSLVQTAFEHNVPIFCPAFSDSSAGFGLAKHQLEKPEKHVSIDS
                     VKDFVELTKIKMEAGDSGLFMIGGGVPKNFAQDTVICAEILGKDVPMHKYAVQITVAD
                     PRDGACSSSTLKEASSWGKVDTVYEQMVYAEATSVLPLIISYAYHKGDWKNRKKWEWA
                     KLFDK"
     gene            complement(15669..16523)
                     /gene="speB"
                     /locus_tag="PK345_02005"
     CDS             complement(15669..16523)
                     /gene="speB"
                     /locus_tag="PK345_02005"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HPX33826.1"
                     /translation="MDCDLNYGGLDADYSGLEHASVVVLPVPYDETSTWGKGADRGPE
                     ALLKASANMELYDIETDSEVYKIGIFTAPPVLEKSSTEIMINAVHKETLKYIRQGKFV
                     VGIGGEHSITTGFVRAHAEVYNDLAVLQLDAHSDLRPEYNGSQYNHACVMSRVAEICP
                     FVQVGIRSMDSIEKPFLVKENLFLAEEICGRNDWYNKVIDQLSANVFITIDLDVFDPS
                     IMPSTGTPEPGGLSWYEVIKLLKFVALKRNIVGFDVMELAPNSSNKAPDFVAAKLIYK
                     LLSYRFKL"
     gene            complement(16870..19728)
                     /gene="uvrA"
                     /locus_tag="PK345_02010"
     CDS             complement(16870..19728)
                     /gene="uvrA"
                     /locus_tag="PK345_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014299224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPX33827.1"
                     /translation="MSLVKIDDLDSKNFILIKGARTHNLKNIDIAIQRNQLVVITGVS
                     GSGKSSLAFDTLYADGQRRYVESLSSYARQFLGRMQKPEADYIKGISPAIAIEQKVNT
                     HNPRSTVGTSSEIYEYLKLLFARIGKTYSPISGQEVKRHSVTDIVDFIESIPEGIPVI
                     IGSEIEQNTGRNLSDQLQILRQQGFTRLFQNGKIIKLDDLLNQIQAVGDLTCEKSNSK
                     TQGLPQLQIVVDRLTVDANDKDQRSRLADSVQSAFFEGHGECFVHIFNADKYETKVFS
                     NRFEADGIEFEKPSENLFSFNNPYGACRTCQGFGNVIGIDEDRVIPDRSLSVYQGAIA
                     PWKGEKLGLWKEQLVLNAHKFDFPIHKPFYQLTQEQQQLLWTGNEYFEGINEFFRYVE
                     QQTRKIQYRIILSRYRGKRICPECRGSRLRKDASYVKINGRSISDLVMIPLEDLLSFV
                     RQLELSKYEKEVVHRVMIEIENRLVFLCDVGLGYLTLNRLSSSLSGGESQRINLAISL
                     GSSLVGSMYILDEPSIGLHPRDTHRLIRVLKKLKELGNTVIVVEHDEDIIRASDQIID
                     IGPLAGNLGGEVVFSGTLNELLTDDKSFTAKYLSKKMSINIPDKRRKWKDFIDFIGCR
                     ENNLKHINVRIPLNILTVITGVSGSGKSSLVKGIIYPGLNNLLGNSSEKATKYDKILG
                     EVNQIKEVNFVDQNPIGRSSRSNPATYVKAFDEIRVLFSEQKLAKVRGYKPGFFSFNI
                     PGGRCDECEGEGTVKIEMQFMADIYLTCEACGGKRFKDEVLNVTFQEKNISEVLDMTI
                     DEAIMFFDKTKQNTIIKRLLTKLQPLQDVGLGYLKMGQPSNTLSGGEAQRIKLASFLT
                     IGDKNNHSLFIFDEPTTGLHMYDISKLLDSLNALIENGHTVLIIEHNSEIIKSADWII
                     DLGPEGGDKGGEIVFEGTPEELINSKISYTGKYLKSKF"
     gene            19946..20533
                     /locus_tag="PK345_02015"
     CDS             19946..20533
                     /locus_tag="PK345_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPX33828.1"
                     /translation="MKAQILSDKELINQYLEGNQYSLEKLIHRHQSRVYAYILMSVKD
                     KQLADDIFQDTFIKVIKTIRSGAYKEEGKFIQWVMRIAHNLIIDYFRKAKRIPLINND
                     NSEFDIFDTIKFTDPSIEEQIITDQIHEDVRSLIELLPAEQKEVLFMRHYAEMSFKDI
                     AEQTDVSINTALGRMRYALINLRKLIKEKNVILTK"
     gene            20624..20890
                     /locus_tag="PK345_02020"
     CDS             20624..20890
                     /locus_tag="PK345_02020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33829.1"
                     /translation="MQNFDTLNDLIFFAYNAPGNHETMKYHQLIIDDKNLARKYQEIL
                     KAKAYLNNIQIGPSEVTIKNIMGFSRALAMSNSTTIGVFDLLLN"
     gene            20964..21623
                     /gene="nth"
                     /locus_tag="PK345_02025"
     CDS             20964..21623
                     /gene="nth"
                     /locus_tag="PK345_02025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HPX33830.1"
                     /translation="MTKKERYRLFIEYFVAHQPDVTTELRHTNPYQLIVAVILSAQCT
                     DKRVNIITTEFYKKFPDVNSLAKAEFDEVYELIKSCSYPNNKTKHLIGMAQKLISDFG
                     GVVPVELEELQKLPGVGRKTANVIASVAFGKPAMAVDTHVFRVSARIGLTTNSKTPLE
                     TEKQLVKHIPEKYISSAHHWLILHGRYVCIARRPKCRECKISHFCKYYKTVYLKESSK
                     N"
     gene            21696..22160
                     /gene="bcp"
                     /locus_tag="PK345_02030"
     CDS             21696..22160
                     /gene="bcp"
                     /locus_tag="PK345_02030"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HPX33831.1"
                     /translation="MAKLKIGDKAPDFKGVDQNNNSISLSDFIGKKVILYFYPKANTP
                     GCTAQACNLRDNYDDLLKKGFKIIGVSADSVQKQNNFASKYQLPFPLIADTEKEVLKL
                     YGAWGEKKMYGRTFEGIIRMTYVIDEKGIIEQIFNKVKTKEHSEQILEAMKQ"
     gene            22177..23202
                     /gene="recA"
                     /locus_tag="PK345_02035"
     CDS             22177..23202
                     /gene="recA"
                     /locus_tag="PK345_02035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013063920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HPX33832.1"
                     /translation="MAKENQESIDKIKAEKLQALERTLGNIEKHFGKGTIMKLGDAPV
                     ENIPSISTGSIGLDAALGIGGLPKGRVVEIYGPESSGKTTLAIHAVAEAQKLGGIAAF
                     IDAEHAFDRFYAQKLGVDIQNLLVSQPDNGEQALEIAENLIRSGAIDVIVIDSVAALT
                     PKSEIEGEMGDSKVGLQARLMSQALRKLTSTISKTGTICIFINQLREKIGVMFGNPET
                     TTGGNALKFYSSVRLDIRKIGQIKDAENISGNRVRVKVVKNKVAPPFKSAEFDLIYGE
                     GISKLGEIIDLGVDFEIIKKSGSWFSYGDTKLGQGRDTVKKLLADNPELADELESQIR
                     EVLKSKE"
     gene            complement(23275..24297)
                     /locus_tag="PK345_02040"
     CDS             complement(23275..24297)
                     /locus_tag="PK345_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HPX33833.1"
                     /translation="MIYRSKSPLRIGLAGGGTDVAPYSDIYGGAILNATISMYAYATI
                     EPLNGNKIIINSIDNNELVEFNSTNEISLDGKLSLHKGVHNRVTKDFGLKNLSYKLTT
                     FVDAPPGSGLGTSSTLAVTILGAFAEWLKLPLGEYDIAHLAYQIERNDLKMAGGKQDQ
                     YAATFGGVNFMEFYKDEKVIVNPLRIRSKFLNELSHNLILYFTGTSRLSSKIIETQRQ
                     NVFSQNKQSIEAMHQLKKQAVMMKEAVLKGELEEIGEILDYGWRFKKQMAEGITNPLI
                     DKIYESAMLAGASGGKISGAGGGGFMIFYCPGNSRSEVIKAVEAFGGQVKRYEFTERG
                     LTSWSI"
     gene            complement(24284..25453)
                     /locus_tag="PK345_02045"
     CDS             complement(24284..25453)
                     /locus_tag="PK345_02045"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPX33834.1"
                     /translation="MSEKTHVKNVIILGSAHPLRGGLAAYNQRLAQALLEQDYNVKII
                     TFSLQYPNFLFPGKTQYSTEPKPENFDIEISVNSVNPLNWIKIGRRIKRLNPDILIIK
                     YWIPFMAPCLGTIARIARKNRKTKVISILDNIIPHEKRIGDKMLSGYFVRSVDGFIAM
                     SKEVMGDLDIFDRKRPRLFCPHPLYDHFGELQSKTIAKERLGLDQESRYLLFFGFIRD
                     YKGLDLLLKAMADERIRKRNLKLIVAGEFYSDPKPYFQLITENQLADQVIMANEFIPD
                     NQVADYFNCCDIVVQPYKDATQSGVTQIAYHFEKPMITTNVGGLAEIVPDDKVGFVVN
                     PDTAEISEAIERFYQENKEESFTKQIKVEKLKYSWERMVEVIEHLIRKTDQNDIS"
     gene            complement(25446..26393)
                     /locus_tag="PK345_02050"
     CDS             complement(25446..26393)
                     /locus_tag="PK345_02050"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPX33835.1"
                     /translation="MDLSLVIPLYNEEESLPELSTWIEGVINKMGLTYEIIFVDDGSN
                     DNSWEVIEMLNHKNPNIKGVRFRKNYGKSAALNRGFFLAKGEVVITMDADLQDSPDEI
                     PDLYKMINEKGFDIVSGWKKKRYDPISKRWPSKFFNWTVRRISKLPLHDFNCGLKAYR
                     KDVIKSIEIYGEMHRYIPVIAKWGGFRKITEKVVQHKPRKYGSSKFGAERMIKGFLDL
                     ISLTFVSKFGKRPMHFFGVIGSLLFIVGLIIAIYLTYAKFFLQGYRMTERPLFYFGLL
                     AMIMGVQVFSAGFVAEMISRNSPRRNEYEIEKTSGITNE"
     gene            complement(26403..26933)
                     /locus_tag="PK345_02055"
     CDS             complement(26403..26933)
                     /locus_tag="PK345_02055"
                     /inference="COORDINATES: protein motif:HMM:NF025227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4199 domain-containing protein"
                     /protein_id="HPX33836.1"
                     /translation="MEEIKPSSFRVAINQGLLLGLALIIFSLILYVAGTGPKSSLNYL
                     TYLIIVIGMFLSMKQWRDKFNNGFITYGNAFKHGFYVILITGILTAIWTFVFYNYIAP
                     GEVAKIIEATEENLYKSQPNMSEEQVEMALKWSKMFVSPKIMALTGFFGNIILGVILG
                     AILSFFIKKEKPAFSE"
     gene            complement(26986..27354)
                     /locus_tag="PK345_02060"
     CDS             complement(26986..27354)
                     /locus_tag="PK345_02060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33837.1"
                     /translation="MIKYTSADQLTIEKFKTPFEINLYRYNRLVKLSQIIPWDEREAI
                     ETKEEQTIGNNIEGKFGQEKTAYNRARIHTAKSSESWSVCIMFSMKLIKFIKIFLFSF
                     AKWFKNRWGRFFLCLSRSKN"
     gene            27567..27666
                     /gene="ffs"
                     /locus_tag="PK345_02065"
     ncRNA           27567..27666
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="PK345_02065"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(27717..>28011)
                     /locus_tag="PK345_02070"
     CDS             complement(27717..>28011)
                     /locus_tag="PK345_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF5103 domain-containing protein"
                     /protein_id="HPX33838.1"
                     /translation="EGKIYLMGGLTQWQFLKENELTYNFERRGFESSILLKQGFYNYQ
                     YIFLPNNSQVGDVTLIEGSFYETNNEYTFYVYYRPKGTRYDQLVNITHIISHP"
BASE COUNT         9052 a         5136 c         5077 g         8746 t
ORIGIN      
        1 ttatccgtag ttgggcaaat catattgcaa ttgttaaata tgtgttaatg ttttgagttc
       61 tattaattat taaaatagat ttgcctgctc aattatttag agattaatca tgaaaaagat
      121 tatactttat cttttactta tccttgctat gtttaaatgg ccggcttttt cccaaacaaa
      181 gatctttgat gaatcttttg aatcggattt gaccggatgg aattttgaag gaaattggtt
      241 tcaggagccg ggttacattt ttatgtacta tcaccctgta acttacaatt acgacttctg
      301 gactatctcc cctgagtttc aggtgccggt aacgggcggc gatcttatta ttaatcattt
      361 tgttgatgtt taccaagcaa acgttactga tgagaaatgc gaaatcttaa ttttacataa
      421 cgatcaagaa gatgtagttt gggaatatgc tttgagcaac ggaacttggg gaagtatttt
      481 tggcactgac atgcttatcc cattagacga attcatagga gagacggtaa gggttaaaat
      541 taaatcttat ggtgcaaaat ccaacgctct ttggggctgg ttcatcttta atatgagcct
      601 gacaactttt tttaattatg acgttgaagc attgcaatta aatggccctg caagtcttaa
      661 tcccggtgaa gtcggggact ggacattaag cataaaaaat ctggggctta atccaatatc
      721 cgatattacc ataaagcttt ttagttataa agagtataat gaacttgcca cagaaatctt
      781 taatcaatct atacctgccg gcgaaaccag cctggcacca attacctgga gtagcaattt
      841 ggtgcacaac acaatgcttt atgccgtaat tgaacacacc aacgatcaat attctgccaa
      901 taataaatca caaagcaagt tcttgcgaat caatccgcca caggaggtta atattttagt
      961 atgggacaat gataacggaa tagagaccat tatcaatcct gaaactggtg ttaatcaaca
     1021 agctagcgct acaatagagc taaatttaca agaggcaggg ctccaatatt cacttttaga
     1081 aaaactacct gttgatctca gccaatatga tattgtaata gccacaatgg gcagccattg
     1141 ccttgggtga agcacgaccc cacctggtac tgtaaacgaa gcagaccagg ccatactcct
     1201 taattaccta aacaatcaca atggtaatct atatatcgaa agtacaaagc tgggatttga
     1261 tcattatgta acagatcttt tcgcacattt tgggataaaa ttccatagcg agggtgaagt
     1321 atatgaagta gaaaaggtta tttcaaatag ccaaacgtta atgaataatg ttgactttac
     1381 ttatgcaggt ggaaatgaac cacattatac cgttgaccgg tttctgagta atggtgcaga
     1441 aatgctttat acttccgaag atggatatca caggatgttt gcctacaacc ctgatagtag
     1501 ctataaagta atttcatcct ctgttctaat tggagcgctt aaaaatgcgg acagccttag
     1561 tacaaaacca tacctattag ccgaaatagt taattatttt ctcggtatag aaaccgcaac
     1621 aaacatccgc gagctgtttg acaaaaaaat tgccaatagt gttacagctt acccaaatcc
     1681 attcagtagc agtacaacaa ttggttttac acttaagact gagggtagtg taaaggctga
     1741 tatttttgat gtaaatggcc gccatatcaa agcatttaaa caacaagtaa tgccatccgg
     1801 ccagcatgac tttatctgga atggaagtaa cgatgcagga accgtagggc aaaatggagt
     1861 ttatttttat caaataaata ttgacggaaa aattgaatcg ggaaagattg ttttaaaccg
     1921 ttaaccgtca ctaaaagtta tcattaaaag gataccaaaa taaaacttgc gcctcctttt
     1981 ttttattcag attaagcatt tgccaacaca tagtcaggat taaatcaata gatctgtcca
     2041 aaagagctat cagttgatta agtagagttt gtttttaaaa ggttattact tttgttttta
     2101 gttaatacac tattctatca tcaaaaaatc agatggaaga gagactaaaa aaaatacctg
     2161 gtgttgatat tttactaaac aagcctaagg ttaaggattt actcaatcgc tatccaagaa
     2221 gcctggtgcg tcatattatc caattattat tgaaaaatat ccggatggaa gttctctcag
     2281 gaggaaaggt gcctgacgaa gaagaaatag ttaatgacat cagaattaaa acggaatctt
     2341 ttagcagtaa aagtcttcga ccggtgatta actgcactgg gataatcatt catacaaatt
     2401 tagggcgggc tccatttggc gaccagcttc ttgctgatgt acagcatctt ttaagcggat
     2461 atagcaatct ggagtttgat ttggaaaaag gggaaagagg ttcgcgatat gtacatgtta
     2521 cagagcttat gaaatttctt accggtgccg aagatgtttt ggtggtgaac aataatgctg
     2581 ctgctgtgat gcttatctta agaactttcg caaaagataa agaagctatt atttcaagag
     2641 gagagctaat tgagatagga ggttctttcc gtatgccgga tattatggcg gcatccgatt
     2701 gtaagatggt ggaggtggga actaccaata aaacaaaaat taccgatttt gaaaatgcaa
     2761 tattaaaaga tacatcaata attctcaaag cgcataaatc gaactatgtg ataaaaggat
     2821 ttacaacgga accaccatta tccgaattag ttgatttagg caaaaaaacc ggagtaatgg
     2881 tagtttacga tatgggatca ggtttgttaa gaaaagcaaa agttaagatg cttgacgatg
     2941 aaccagatgt cagatcagcg cttgccaccg gagttgatct tgttactttt tcaggagata
     3001 agctactcgg cggcccgcag gcaggaatta ttgcaggaag aaaaagcctt attgaaaagc
     3061 ttcgcaaaga acctatgacc cgtgctctga gagtaggtaa aacagtgctt gccttattag
     3121 aatcaatctg tatgatgtat ctcgatgaaa agttacttat agataagagc cctctttttg
     3181 ctatgatgaa ttcaaccatt gacacacttg agaaaagagc attgctactt gccgatgatt
     3241 taaaaaagag agatgttgga tcaaaaataa taaaaagcgc tggtcaggcc ggaggcggtt
     3301 cgctacctga taaaaccatt gaaagttacg ctgttgtacc gcaaattaaa gctaattcaa
     3361 gagcggaaaa aaatgaaaag gcaaaatgga tctatcatca gcttctcagt caaaacacac
     3421 caatacttgg agcgctgcgc aaaggagagt tgatttttga tgtactcacc ctttccgata
     3481 aggagattaa aatagtagcg gaaattattg caaacacata tcaggaggct aatcgtgtct
     3541 aaacatttga tcatgggaac ggccggccat gttgatcatg gtaagacttc actaattaag
     3601 gcgcttacag gaattgacgg tgacacccat aaagaagaaa aaaaacgggg tatcacgata
     3661 aatcttggat tttcccacct cgatcttgac gataatatat ctctcggaat tgttgacgtg
     3721 cctggccata aagattttgt aaaaactatg atagccggag cgcatggtat tgatttcgtt
     3781 ttgcttgtaa tagctgccga tagcggaata atgcctcaaa cacacgaaca ttttaatata
     3841 ataaaaatgc tcggtattaa acatggctta atagcgctca acaaatccga ccttgttgat
     3901 gacgaaacca ttgaacttgc caagctcgag attatggaat ttgtggataa cagtacatta
     3961 gagcatgccc ctattgttcc tgtttcagca actaccggct ttggacttga agccttaaag
     4021 tccgtaataa aaagagttgc cgaaaaagtt gacacaaaaa cgactactaa ccagttcagg
     4081 atgtatgttg atcgaatttt caatgttaag ggtattggca tcgttgttac cggatcggtg
     4141 ttaggagggg aggcgagcat aggggaggag ctttttctgt tgcctggaag tcataacaag
     4201 ctaaagataa aaggtcttga aaggcatggc aggcaagtgg aaaaaatcat tgccggcgat
     4261 agagcagcca ttaacctctc aggactgaaa tataacgatt ttgaaaaagg gatgatcctt
     4321 acaaaaatta tcttgccaga aataaaactg cttgatgtag tattagagtt atttcccggt
     4381 aatcaaaggg ttggaatatg gagccagatg atttttcata ccggaaattt ttcatcaaaa
     4441 gccaggattc atttgctaaa caaaaacgaa attaatggta gtgaaacagc ccttgctcaa
     4501 atccatttgg agaaaccagc tatattgctc aatcgcgatc gttttgttat tcgaaatact
     4561 tccaatgatc ttaccatcgg cggtggaata attattgaca accgtccact acaccataaa
     4621 aagcgcacca aaactttatc ccgttatctt gaaacacttg cacaagctac aactgaccat
     4681 gaaaatctat cggcgctaat tttattggaa ctcaataagt cagcattacc gttaacaacc
     4741 gaaactattg cttcaacact taataaaaca attaatgagg ttgaaaatgc tattttggat
     4801 gtcgaattac aatcggctat acttgtttac gaaaacaaaa attatcttat tcctaaagca
     4861 ttcatcgaaa aaaataataa aagaattatt gaaacattaa aagtatggca cttgcaaaat
     4921 ccattaagtg aatctggttt agaattaaga gagatagccg gaaaggtaaa aattatgggg
     4981 gatttcaaca tatatatgct tggtaaaatc ctcgaaaaga tgacttttga aaaagtgatt
     5041 cgtactgtgg gagctacttt tgtgttaaaa gagcataagg tgtcattcga ccgtaaaaca
     5101 caagaacaaa tcatttggct tgaagagatt attagggatt cgggtatgca aagggctaca
     5161 attaatgagg tagaagcgat agccttgaca aggaatataa aaaaaggtca gcttgtaaaa
     5221 ctattgcaat atcttgctgc taacgataaa atctttttca atggtgaaga tatccttcac
     5281 aatagtctgg ttcggaaaat aaggttaaaa cttttacaaa acctttccga aaacccacgc
     5341 ggaataaatg aaaaagaatt tagactattg gttgatggta ctaaaagggc aatacaagag
     5401 cttgtcaact tatttgttca agaagggatt attgaaaaac aaacttttta tctcttaatt
     5461 acggaaaagg gaaaagaact acttttgtaa agatcaagcc ttagtattat ccttttttaa
     5521 ataaacccca tctgcttttt tttcgtacca atatataaaa tacataagcg ctattgaaag
     5581 tataatgcta ataatgagta agattgggtt gtccactttg ccgaattcat caacagaaac
     5641 ctcaataatt cccctattca tcaaaaaaga gattattacc gctgaagcat tatttataaa
     5701 atgtgcgata attggtaaaa aaagcgatcc tgaccatacg aagagataac caaaaacaag
     5761 cccgagcagg aaacgaggga gaaaaccata aaattgaaga tgaaaagcgc tgaagataaa
     5821 agctgaaata ataactgccc aattggagct tttaaaccac gacctaaaca ctcttatcag
     5881 cacagctctg aataacaact cctcgccaac agcagctaaa acaccaatta ttaataagtt
     5941 tatcaacaac cctttaatgg aggtcgttcg cataaaggct tttgtcatct cttctgctct
     6001 catttccgat acccgcatcc actcttcaac cgcgcttaaa cacgatggta gcgacatgtt
     6061 ttgattaatc cgaaccaatt catttataac gggaagcaac agataaataa gaataatggc
     6121 tgataaaact ggaagtagat taggtttttt ctgtaaacca agatactccc tgatatccgt
     6181 agaaacaagg aaagcaaaaa gaaaaggtgg gataagaaaa gttccgaact gattaattaa
     6241 ttgcatgaat ttcaggagat taatctcgtt tcttgtttcc ggaatagcaa tacctgaaag
     6301 agtttcaagt acgggaatgc caaaaaataa acgagaaaga actacaccta aaaaaaagct
     6361 gacaatccag aaacaaagta cgagtaaaaa caatacaacc aattgaacaa cagcaggttg
     6421 atggaaaaat aaaggagtac gtctgatcat tttcttttag ttgaaaacaa gtggacaaac
     6481 ttagcaaaat tcatcaattc aacgaccatt caacattgta agcggtaagt aataatgttg
     6541 atttaactct aaaaaacatg atatcttcgg ttttattaca gttgattatt ttcgaataaa
     6601 aataatattt ttgcatccct tttaagccac cttagctcag tcggtagagc agcgcattcg
     6661 taatgcgcgg gtcgtgggtt cgagtcccat gggtggctca tcaaaatccc cttattgtat
     6721 taaagcgcta cgggcaactt atctttgccc gaaaagagtt atttgttgtt catttctttt
     6781 agtaaattga aagatgacaa acgtctttcc agaaatgaac ggggatttgt ttttagattg
     6841 ttccaatccg aagttctcaa tcaataactt cggcaagcta tgatgatgtt ttgcggttaa
     6901 atcacatgca aggccaacta acataattgt ttcaccggtg gtagttacta tgctgtgtaa
     6961 caccgaaaca gattaataat taaaatagat cgagcaatat aaattggtaa atggttaaat
     7021 ttaccttcat cttttaaaga tttgcaaaca cttaatcatt ttttttaagc ttagatcata
     7081 actatcttat taccaatatt ttgataagtt ttaataagga taaaagaagt ttttttggtg
     7141 tttttttcat aaaaagcttg tcagaatagg aataagagtt atttttgcag ccgttttcaa
     7201 aattattttg gcccgttcgt ctaggggtta ggacgtcagg ttttcatcct ggtaacaggg
     7261 gttcgattcc cctacgggct gcaacgtctt atttacaatc ttttgttgat cccttgccac
     7321 aagggatttt tctttttatt aaggctttgt ccggaagttg attattagtg gaaagacaat
     7381 atgtttatag gagtgattta tggtaataaa aatttgtaac tcactcctcc cgaaaaggca
     7441 tcaaatccct ctaatccacc aagcagaaca atatgtttta aaatagtaat tctggcgccg
     7501 acattccatt tatcagcatc atattcaact atcagttcaa cgggtttcag gtgatatggc
     7561 gtaattttta caccaccgaa aaagccaata aactggtagt cgcttgcctt caatacatca
     7621 ctaccatatc ctgtcgtgaa tccaatattt tcaaatacat gatttacttt aaagtttttt
     7681 gtagcaacaa gatatgtgga attaaaatgt gatgctgcac cactggaaag agttgtaaaa
     7741 aaactatgca cacctattaa tatagccgga ttatatctcc cttcatcaat cactcttaat
     7801 cttaccgaag ccattctgtc tcctacagta gagctataat catccaggcc aatcatacgt
     7861 gtaaatctaa ttgaaacctc ggcaaatggt aagaaactgt ttgtaataaa aaagtccatt
     7921 gcatcatggg ttggtcttcc ccacttttta tattcccgat ttagcaagtt ggtcccaata
     7981 aacattgagc ggtcaggata gagttcagcg ctaggtatat tgtaatagcc tgttgtgccg
     8041 gtaagtgcct gagatcttac catgaatgga gaaaaaaaca aaaaaacaat caataaattt
     8101 aaaagcagaa aataaaaatt agctcccata aaggatttga gcttacttga tatggaagat
     8161 tctactttat ttgtcgcgca catcgtatga tcatttacta cgtgcatttt taaaaatgat
     8221 attcttcaat gcagcaaaaa aatattttac atctgttctg aaaggatgtt tctcataagc
     8281 cataagatat ttccattctg actccatgat ctcttcgaaa gattctggca tatcggcata
     8341 ataggggggt atcaggcctg gtttgaattt tattctgtac tgtttcaact cttcgctata
     8401 taaattataa taatggctac tcaatggccg cacacctaca atttttatat ctcctttcaa
     8461 cacgttaata agcattggca actcgtcaag ccaaaattta cgtagaaatt tacccaatgt
     8521 agtaacccta aaatcatctt tgaacttgcc tcctttttcc aacctatttt tctcatacac
     8581 aaattgttgg atatactcag aataggggtg catagttcgc attttatata cataaaagta
     8641 cttacctccc tttccaattc gccttaatct taccaatggg ccataagtcg ggtcagaagg
     8701 ataagatggc tcttttacct tctgggaaac gaaatataat tttccgttaa tatccttatc
     8761 atcaagtacc gtaaaaccgc aagaataaag cctcccaaat gtttcggcct gtgagagtgc
     8821 acgattttta ccctttgtaa tccaaaaata aaagcgtttt aaaatggaca atttcggaaa
     8881 cacccgtcta aaaataacgt caaaagtata tactacataa ttaaatggga agaggtattt
     8941 gcttattatt ctttttttac gaagagtgta agtctctacc ctgccaataa aataaccctc
     9001 attaagaact ttatcattaa ctgtttcaaa aaacttatta agccatctaa aatcattgat
     9061 tttatgaaaa ttaactatac agccatactg tttttcgggg agtgcaaaga tgttaaacct
     9121 tgttgttgtg gagactactt gaaccggccc tgaaacagtc attaaatatt cggaaataaa
     9181 atctttaaca tctttcccgt actctgattc tatcatttct aaaattagct tcacctcaac
     9241 ctggcggtct gaaacatccc ttttttttat aaccggaggg gaaactaaag tatcggtttc
     9301 aggaaaatcg ctcgaacgga attctttaaa atcattttca taatcaatta cttttgaagt
     9361 atggacatga ttataaatta gcgctgctaa aatttctaat actgtagcag ttaaaatggt
     9421 tccaaaatag ataagacgag atacattaaa aatagtaaat gtatataaaa ctaacaagcc
     9481 taaagcaagg ctgacaaaat ttgattttag aatgatatat aatatttccc gacgatttat
     9541 gtatttttcg gaattgtatt ttttgaaatg aaaagaaaag attagccaag taattaggaa
     9601 aagaacaaaa ggcaatgaat atgttggcaa caccacggta acagtggcag atttcaaaca
     9661 agcaacaaac aagaatgaaa ccgttagaaa gaaaacatca aacaatacaa aatgtaaacg
     9721 tcttttcata aatttgaatt agcgggtgaa gatactcatt agtaatttag tgaataataa
     9781 tcattatact tttttaagcc atgataaaaa attgtatcca aattctgcaa tagaatattt
     9841 aagtttatta ttaattcgaa taaatcgtcc gtaattaact atcgaaccgc caaatcttaa
     9901 tttaaaatcc cgaactccat agggttcgtt gggtttgcca acacccataa aatcaaaaac
     9961 agaacttccc tgctcatgag catattcgat ggctgcccag gttgccaata ctgaaggata
    10021 aataccttct tttttatatt cgggatccaa tccacataca taccattcaa aaactgtttt
    10081 tgccggaagt gatggcgcca ttatccctcc aattattttc tctttatatt cgatcaaaaa
    10141 atatttacca aaattatatt tctgaatcag gaaatagaaa gcttcgaaaa aagtccaatc
    10201 gggtaatggt ttacgaactt tttggcgata aagtttttga agaatatcaa aaaactgacg
    10261 gacttggcct atattgttgg gttgaataac ctttgcacca ttatttaagg atttcctgat
    10321 ttgtctcttt cgagatgaag acatctcgga aaatattttt tccttattct gagtattgat
    10381 taaaagatta attctgggaa cccattgaaa cccattttct ttaaaaattt ctgtataaca
    10441 cgacagatca tgcaaagctc taaattgagt aaaaattgct gtgcggcttg ttttatcaat
    10501 catcgaagct aacatcattc taaatatctc cttgtcataa acatttcctg attcaattaa
    10561 cggccctcca taaattacaa gccgcgctga aaacagagat ttgatatagc ttttttcccg
    10621 gattaacaca gcgagcaaca caccaaccat ccgatcggtt tcgtcaatac aagccacgaa
    10681 aaaaggattg aaatttttgg ttctgaaaaa tagttgccat gcgtaccagc tttgaagaac
    10741 agacccttcc ggatgtgcct ggacaaaccg attccaatct gaaactattt tttcagaaac
    10801 ctcagttact attcggtatg agcttttttt gttacttttt tcttccaaat tataaaacca
    10861 tctgcaaata aaagtgcaaa attaagatta aagctgtgtt gtttaagaga atcattaaaa
    10921 aatgatcttt ttcaatctgt ctctttgttt gttaaattgt taacaaaaca tatgtttata
    10981 tctaatttta tactttagca gcattaataa aattaactaa attcatcaat ctatgaaaaa
    11041 agttttttct gtttttcttt tctttatcac catttctgtc tattcgcagc aattatctgc
    11101 tgatgggcgg tgtatgcctg aggatctttt acaaaacaca attcttcagg gacgtaatga
    11161 aatgggaaat tttaccatta ccgatagtga tggtgttacc cgtaccctat atgacgagtt
    11221 ggattctggt aagactatcc tgctcgatct gttttttacc acttgaggta cttgtcaggc
    11281 gtatgcagga ataattgaaa ctgtgtacgt caattatggg caaggccagc aaaatgtaga
    11341 tttctgggga ctttctcctt atgacgacaa tgtacaaatc aataattaca aagcacagaa
    11401 tggcattacg tatccgtgtg ctggatatca aggcggtgca gcagcagcca taaacattgt
    11461 tatcagcggg caaccttttt tagggtatcc tacatgggta gtaatttgtc cggatcgttc
    11521 ttttacattc gatgtttgtt ttcctccttc tataacttgt tttgatccat attttgctgc
    11581 ttgtggtccg cctatggctg ctgattttat tgctgatcaa actcagatat gtgaaaccaa
    11641 ttttgttcag tttaccgatc aatcaatagg taatccgacg ttatggatat gggactttcc
    11701 aggaggcaac ccatctacat cttccgaaaa aaatccgctt gtggaatatc caaatgcagg
    11761 tcaatttgat gtttcattaa caataaccaa tggtatagcc ggcaacacga ttactaaacc
    11821 taatctaatt gaggtggatc catctcctga agtttatgca ggtttaaaag gcgagacctg
    11881 tgttaatgag cacattacag ttactgatgc aagttctcaa aatacttcaa tacttttgtg
    11941 gcaaatagtt gcaggacaag gtatcttaga gaattctcaa tccatatctc caacatatat
    12001 tccggctcca gaagatgccg gcacaacagt tacacttaaa cttactggta atggcagtgg
    12061 agaatgcgca tctatgaatg ttgagtcaat aaaagaaatt gatatttggc aacaacctga
    12121 agttacactg atgccatttg atacgatatg ttatcaatgg gatgagtatc ctttagttgg
    12181 aggataccct gaaggtggtg tctattcagg cactggagta attgacaata ttttctatcc
    12241 acagattgca ggtgtaggat ctcatctgat aacttatcaa tgggttgact tgcaaacagg
    12301 atgttcaaat agtgcagagg aggtgattgt ggtagttatc tgttcgggta tttggaatgg
    12361 tacagaattg ccaatattca atatttaccc aaatccgaca aaaggtgaat ttgtagttga
    12421 attagtccag tcaggcaatt atgagctaac tattactaat attgccggaa taaaagtttt
    12481 ctcggcatat ttaaatgaaa gctctacaat taaacttgat aatgtggaaa acggtattta
    12541 taacgtaact atctatgatg gcaaaaagtc tcacacaaaa aagattacca taaaaaaata
    12601 attgcatttt ttttaaacat ttttaaacag atttccattc tctttttgga aatctgtttt
    12661 ttttaatgta gaggtgttat ttaactattt agtcaatcct taaaagcatg attaagtgct
    12721 gtttatcaac actatactgt taataacaat atttacttta ggtgttcttt attgcaaggt
    12781 tttgagttat tttagcaatg aaagtattga agatctatag atccgaaacc cttattatca
    12841 atatttctgt atagaggctg agtttcagta gcactttcct tgttatccga gcgatttagt
    12901 acactttcgc aaaaggatcg gtgaagaaag ggatagagtt gattttcaaa atgtcaattg
    12961 atgttcagca ggtaagagcg aatatgaaaa actaaaatga cgagccaaat gacggatcag
    13021 agctgccatc ggaccgatta tcggaaatgt aaaacatgat tgccgaatgg taaaaaacta
    13081 tctcaaaggt gaaattagag acaaaatcaa tgcattgatg gccgcagccg ggttcaattt
    13141 ccgcagatta ctccgcaaac tgaatatgga gattattttt ttcttttttc agattcagaa
    13201 aattctcaac cccaaacctt ctttggcttc taaactcata ctgatgtctt atataccctc
    13261 actgaaagtg agtttttaag gattgactaa gtaatgaatt tttataacag ctttatgaat
    13321 cgtgcaacag ggaataacaa acaaaaaaga ccaagttctg attaatagtt ttaattaacc
    13381 aattctgttt gccaaagtaa ataaaggtat ttttgtacga ggttttctta aagcgacatt
    13441 tgaaactttt taaatatgaa aaaaattgga gttttttact ggcctaaggg cggaaatgtt
    13501 gaatcctgtg cccagcgaat tgctgataga tttaaaaacc aattcgaagt ccaaatttac
    13561 agccttgaca ctataaaagg cctcgacctg ccatattacg accttgttat tgttggcggt
    13621 tcgacctctg gagctgatac gtggcaaaaa ggatcgggag ataatctttg gtttgatttt
    13681 ttcgaacgtt taaaaaacgc ccagctacat ggcaagccgg tggccatttt tggacttggc
    13741 gatcaggtgc tttaccccga tcactttgtt gacagtatgc aaattataaa aaaagaattt
    13801 gatcttattg gagctaatct cgttggccgc tggccagttg aaggttatta tcataccaac
    13861 tcagaagcta tcgaagatgg aaaatttatt ggactggcat tagatgaaga tcagcaacct
    13921 gaacttaccg accagcgtat tgataagtgg gttatgcaaa tagttaatga atcaggatta
    13981 taaaaaaatc acccaacaat attttatcaa ctctttagta ggtacgaaaa atagttttct
    14041 aagagttaca aaatcgagag tagctcaatt tcgaatataa taacagaatt gggaggtatg
    14101 gctccaacct ggttactacc ataacccata gccgaaggaa ttaagaaaat acccgatcca
    14161 ccctctttta acattggaat cccaattctc catccttcga tcagattttt aagtttaaaa
    14221 acccttgcat cctgatttgt ctgatcaaat atttcaccgt tagtaagata tcctttatat
    14281 aggactttaa ctgttgagtt gacggttgga tgagcgccag aaccctcttc gataatgata
    14341 taatacaaac ccgactcatg gcgcaatgct tctaattggt tagtagccag ataatctttt
    14401 atgatctgtt catccttttc agcttgtttt ttctttatgt catccttttt gcatgattgg
    14461 aaaccaattc ctaccaataa aaaaactaac aaaacttgat taattagcaa tttctttgtc
    14521 atactaaatc cttattatga ttcgactggc aaaattagat catttacagt gaattaatca
    14581 atagaaagtt caattagtct atgttgttgg tttaaaacat atttggttat ttatcaaaga
    14641 gttttgccca ttcccatttt tttcggtttt tccagtctcc tttatggtat gcataactaa
    14701 ttatcaatgg tagtacggat gtagcttctg catataccat ttgttcataa acagtatcaa
    14761 ctttgcccca tgatgaagcc tctttcagtg ttgagcttga gcatgctcca tcacgcggat
    14821 cggctactgt gatttgtaca gcatatttat gcattggcac atccttccct aagatttctg
    14881 cacatattac agtgtcctgt gcaaagtttt ttggcacacc gccacctatc atgaataagc
    14941 cgctatcgcc agcttccatt tttattttgg taagctcaac aaaatctttt actgaatcaa
    15001 ttgatacatg cttttcaggt ttctccaact gatgctttgc aagtccgaat cctgcactag
    15061 aatcggaaaa ggcagggcaa aaaataggca cattatgttc gaaggcagtt tgaacaagcg
    15121 aatctttctt aaccgcatgt tttgttaaat atcttcccat ttccttaata aactctcttg
    15181 acgagtatgg gcgtggctca aggctgttgg caatgatctt ggtggtttcg tcgcaagcct
    15241 gaagttcttc ttcgtcaatg tatgtgtcgt aaattcgatc aatataaagt gatcgcagtt
    15301 gcttatcgtc tgcccatgag gatcctatgt agtgtttata tcctaaggct tcaaaaaaat
    15361 ccatatcaac aattgaagcg cctgttgcta ctacaacgtc aatcatgtta tttttaatca
    15421 tatctacgta cacttgcata cacccacccg cgcttgtact tccggccaga gtgagccaat
    15481 ttgtacagtt agcatctttg atcattaact gtaaaatgtc ggcagcctgt gctatttcgc
    15541 gtgaagtgaa tgacatatcg cgcatagagt caatgattgg cgtagaatcg aacgatttga
    15601 tgtcaatatg tttgacaacc ctattcaaaa aatctttttt attcatatcc ttatttattt
    15661 tgattaatct aaagtttgaa acgataggat aatagtttgt aaataagttt agctgcaacg
    15721 aaatccggcg ccttgtttga actattgggc gcaagctcca tcacatcaaa accaacaata
    15781 tttcgtttta gagcaacaaa ttttaaaagt ttgattacct cataccacga tagcccgcca
    15841 ggttccggag ttccggtgga gggcataatt gagggatcga aaacgtcaag atctatggtg
    15901 ataaaaacat tggcgctaag ttgatctatc actttgttgt accagtcatt acggccacaa
    15961 atctcttcgg ctaaaaaaag gttctctttt accaaaaaag gtttttctat actatccatg
    16021 cttctgattc ccacctgcac aaacgggcaa atttcggcaa ctctcgacat aacacaggca
    16081 tggttgtatt gacttccgtt atattcaggg cgaaggtcgc tgtgggcatc caattgcagt
    16141 acagctaaat cgttatatac ttcggcatga gcacgtacaa aaccggttgt aatagagtgt
    16201 tcgccaccaa taccaacaac aaatttaccc tgacggatat attttaaagt ttctttatga
    16261 actgcattta tcattatttc ggtggatgat ttttcaagaa ctggtggcgc ggtaaagatt
    16321 ccgattttat atacttcgct atctgtttca atgtcgtaaa gttccatgtt ggccgatgct
    16381 ttcaataacg cttcaggccc tcgatcagcg cccttgcccc aagtactggt ctcgtcgtaa
    16441 ggaaccggaa gtacaaccac tgaggcatgt tccaaaccgc tgtaatcagc atccaaacca
    16501 ccgtaattca aatcacaatc cattattaat tttttagtta agatttcgac aaatatataa
    16561 aaactctttt catattttgc aatgttagtg tttttgtctt tgaagatatt gataattcat
    16621 tgctaaattt tgtaattaag tacagtttta atgcacctaa taaacgaatt aacaagaatt
    16681 aatcaaaagg aatcgaagtt atttatctaa aatcaaaaga aagccctttt aagaataact
    16741 gttgtttttg atgagaatcc tgatttagtg tttgttaact ataattctta ttgtgttgat
    16801 tacaaacatt tagtagttat ttcaactcaa agccctaaga gatcaaatat tactatgtga
    16861 ataatgtttc taaaatttgc tttttaggta cttaccggta taagatattt tgctgttaat
    16921 caactcctct ggcgttcctt caaaaacaat ttcccccccc ttatcacctc cttccggtcc
    16981 tagatcaatt atccagtcgg ctgatttaat gatttcagaa ttgtgttcta tgatcaaaac
    17041 agtatgtccg ttttcgatca gggcattaag cgaatccaat agtttgctga tatcatacat
    17101 atgaagccct gtggttggtt cgtcgaagat aaataagcta tggttatttt tatctccaat
    17161 tgtaaggaac gaagcaagtt ttatccgttg tgcttcacct ccagatagtg tgttcgatgg
    17221 ctgtcccatc tttaggtaac ctaatccaac atcttgcaat ggttgtaatt tggtgagcag
    17281 ccttttgatt atagtgtttt gttttgtttt atcaaaaaac attattgctt catctatggt
    17341 catgtcaagc acctcactga tatttttttc ctgaaaggta acgtttagaa cttcatcttt
    17401 aaaacgtttg ccgccacatg cttcgcatgt aaggtaaata tcagccatga attgcatttc
    17461 aatctttacc gtaccctcgc cttcacactc atcgcatcgt ccaccgggga tgttaaaaga
    17521 gaagaaacct ggtttatatc ctctgacctt agcaagtttt tgctcactaa agagtaccct
    17581 tatttcatca aaagctttta cgtaggttgc aggatttgaa cggcttgatc gtccaattgg
    17641 attctggtca acaaaattaa cttccttaat ttgatttact tctcctaaga tcttatcata
    17701 tttagtggct ttttcgctac tatttcccaa taaattgttt agtccgggat aaataatccc
    17761 tttcactaac gatgattttc ccgatccact tacaccggtt atcacagtca gtatatttaa
    17821 tgggatccgc acattgatat gtttcaggtt attttcgcga cagcctatga agtcaataaa
    17881 atctttccat ttcctccgtt tatccggtat attgatactc atctttttac taaggtactt
    17941 agctgtgaaa ctcttgtcat cagtcaataa ttcgtttagg gtaccggaaa aaacgacttc
    18001 gccacccagg tttcctgcca gaggtccgat atcaattatc tgatcggagg cacgaatgat
    18061 atcttcatca tgttcaacaa cgattacggt atttcctagt tctttcagtt ttttcagaac
    18121 tctgatgagg cgatgagtat ccctgggatg aagtcctata ctgggctcat ctaagatata
    18181 cattgagcca acaagactgc tccctaacga aatagcaaga ttaatacgct gagattcacc
    18241 acccgataaa cttgaagata aacggttaag ggtaagatat cctaacccaa catcgcaaag
    18301 aaatactaac ctgttctcta tttcgatcat tacgcgatgg acgacctctt tttcatattt
    18361 acttaactca agttggcgaa cgaagcttaa caaatcctcc aaaggtatca ttaccaagtc
    18421 gctaatagaa cgaccattta tctttacata acttgcatcc ttacgtaacc ggcttccgcg
    18481 acattcgggg catatcctct tgccacgata gcgacttaaa attattctgt attggatttt
    18541 tctggtttgc tgttctacat aacgaaaaaa ctcgttgata ccttcgaaat actcatttcc
    18601 agtccaaagc agttgttgtt gctcttgcgt tagttgataa aagggtttat gaattggaaa
    18661 atcgaatttg tgtgcattta ataccaattg ctctttccat aaccctaatt tttcgccttt
    18721 ccaaggggca atggcgcctt gataaaccga aagacttctg tcaggaataa cccggtcttc
    18781 atcaatacct attacattcc caaaaccttg acatgttcta caggctccat aaggattatt
    18841 aaagctaaag aggttctcag agggcttttc aaactcaatc ccatctgctt cgaaacggtt
    18901 ggaaaacact ttcgtttcat atttatctgc attaaatata tgcacaaagc attccccatg
    18961 tccttcaaaa aatgctgatt gtaccgaatc ggctaatcgc gatcgttggt ctttatcatt
    19021 tgcgtcaaca gtaagtcggt caacaactat ctgaagttgg ggtagacctt gtgttttaga
    19081 atttgatttt tcacaagtta aatctccaac agcctgaatt tgatttaata aatcgtcaag
    19141 ttttattatt ttcccgtttt gaaaaagtcg ggtgaaaccc tgttgcctta atatctgtag
    19201 ttgatcagaa agattcctac cggtattttg ctctatttca ctcccaatta ttacgggtat
    19261 gccttctgga atactttcga taaaatcaac tatatcagta acggaatgtc gtttaacctc
    19321 ttgtcccgat attggtgaat aagtctttcc tattctggca aacagtaatt taaggtattc
    19381 ataaatctca gaagaagtgc caaccgttga acgcggattg tgggtattta ctttttgctc
    19441 aatggcaata gcgggagata tacctttaat gtaatctgct tcgggctttt gcattcttcc
    19501 taaaaactgc cgagcatagg aactaaggct ctctacatat ctacgttggc catcggcata
    19561 caaagtgtcg aaggcaagtg atgatttccc tgaacccgac acccccgtga tgacaacaag
    19621 ctgattgcgc tgaatagcaa tatctatatt tttgagattg tgtgtacgcg cacctttaat
    19681 taaaataaaa ttttttgagt caagatcatc aatcttcact aatgacatga tttataaggg
    19741 aatagactta tttttgcaaa aataacaaaa acttaaaaaa tgttattaaa gaaaaaaaat
    19801 tgcaaaaaaa tttggtagta tgaatttata atgtacattt gcaacgattt taagattaaa
    19861 tatcattact aattaaaaat taaaaggaca ggctatcgaa aaaaatttta ctctttgttt
    19921 ataaaaatca aaaaaaggaa cttttatgaa agctcagatt cttagtgaca aggagttgat
    19981 taatcaatat cttgaaggta atcaatatag cctcgaaaag cttatccatc gtcatcaaag
    20041 ccgtgtttat gcttacatcc tcatgtcagt aaaagacaag cagttggctg atgacatatt
    20101 tcaagatact tttatcaaag ttatcaaaac tatccgttca ggcgcttaca aagaagaggg
    20161 taaattcatt cagtgggtga tgcggattgc gcacaattta attattgact attttagaaa
    20221 agccaaacgt atcccgctga ttaataatga caacagtgag tttgatattt ttgacacgat
    20281 aaaatttacc gatccttcaa ttgaagaaca gataatcacc gatcagatac atgaagatgt
    20341 aagaagtttg attgagttac tccctgcgga gcagaaagag gtacttttta tgcgccatta
    20401 tgctgagatg agttttaagg atattgccga gcaaaccgat gttagcatta atacagcgtt
    20461 aggtcgtatg cgttatgccc tcattaattt gcgaaaactt atcaaggaaa aaaatgtgat
    20521 cctgacaaaa taagttttca tttcttttcg caaacgaaat aattatttcc tttcaagcgt
    20581 ttgttgtttt gttaagaatt ttagtttata aacaaaacag cctatgcaaa attttgacac
    20641 tctcaatgac ttgattttct tcgcatataa cgcaccgggt aatcatgaaa ccatgaaata
    20701 tcaccaactg ataatagatg ataagaattt ggctagaaaa tatcaagaaa ttttaaaagc
    20761 aaaagcatac ttaaacaata tccaaatagg gcctagcgag gtaacaatca aaaatattat
    20821 ggggttttca agagctttag ccatgagtaa ctcaacaaca attggtgtat ttgacttatt
    20881 gcttaattga aaaaattaaa aaataataaa aagccactta aagatgagtg gctttttgtt
    20941 tttacacaaa taaaacagaa cctttgacta aaaaagagag atacaggctt tttattgaat
    21001 attttgtagc tcatcagcca gatgttacaa ccgaacttcg gcataccaat ccttatcagc
    21061 ttatcgtagc ggttattctt tcagcgcagt gtacggataa acgggtaaac attataacta
    21121 cagaattcta caagaaattt ccagatgtaa attcccttgc taaagctgaa tttgacgaag
    21181 tttacgaatt gataaaaagc tgttcctatc caaacaataa aactaagcat ttgattggga
    21241 tggctcaaaa actgatcagt gattttggag gtgtggtgcc ggtagagttg gaagaattgc
    21301 aaaaacttcc gggcgtaggt cgtaagactg caaatgtaat tgcttcagtg gcttttggca
    21361 agccggcaat ggctgttgat acacatgtct ttagagtttc ggctagaatt ggactgacaa
    21421 ccaactcaaa aacacctttg gaaactgaaa aacaattagt aaaacacata cctgaaaagt
    21481 atatttcaag cgcacatcat tggcttatcc tacatggcag atatgtttgc attgccagac
    21541 gacctaagtg ccgggaatgt aaaatctcac acttctgtaa atattataaa acagtttatt
    21601 tgaaagagag tagcaaaaat tagcgtttct aatttggtta attcatttat agaaatcttg
    21661 ttattttagc agaaaatgaa atattcgatt ttattatggc aaaattaaaa attggcgaca
    21721 aagcccctga ttttaaagga gttgatcaga ataataactc tattagtctc tccgatttta
    21781 tcggaaaaaa ggtgattctt tatttttatc caaaagccaa tacaccaggg tgtacggctc
    21841 aagcctgtaa tctgcgggac aactatgacg atcttttaaa aaaaggattt aagattatcg
    21901 gcgtaagcgc tgattcggta caaaagcaga ataactttgc aagtaagtat cagcttcctt
    21961 ttccactaat tgccgatacc gaaaaagaag tgttaaagct ttatggcgct tggggagaaa
    22021 aaaagatgta tggaagaact ttcgagggga ttattcgtat gacttatgtg attgacgaaa
    22081 aggggataat agagcaaatt tttaataagg taaaaacaaa agagcattct gaacagattc
    22141 tcgaggcaat gaaacaataa caaaaaatat atagtgatgg caaaagaaaa tcaagaatca
    22201 attgataaga ttaaagcaga aaaattacaa gcccttgaac ggactttggg taatattgaa
    22261 aaacattttg gtaaaggaac gattatgaaa cttggagatg ctcctgtgga gaatataccc
    22321 tctatttcca ctggttccat aggccttgat gctgcccttg gcattggtgg tttaccaaaa
    22381 ggcagggtcg ttgagatata tggccctgaa tcgtcaggga aaacaaccct tgcaatacat
    22441 gctgttgcag aagcacaaaa gctcggaggt attgcagcat ttattgatgc cgaacatgct
    22501 ttcgatagat tttatgctca gaagttaggg gttgatatcc agaacctgct cgtgtcgcaa
    22561 ccagataacg gcgagcaggc gcttgaaatt gccgaaaacc ttattcgttc aggagctatt
    22621 gacgtcatcg tcattgactc ggtggctgcc ttaactccca aaagtgaaat agagggtgag
    22681 atgggcgact caaaggtggg tttacaagca agactgatgt cgcaggcctt acggaaatta
    22741 acctctacaa ttagcaaaac ggggacgata tgtatcttca taaatcaact tagagaaaaa
    22801 attggggtaa tgtttggtaa tccggaaaca acgaccggag ggaatgcact gaaattttat
    22861 tcttcagtaa ggctggatat tagaaaaatc ggacagatta aagatgctga aaatattagc
    22921 ggcaacagag taagggtcaa agttgttaaa aataaagttg ctccaccatt taagagcgct
    22981 gaatttgatc tgatttacgg tgaaggaata tccaaacttg gcgagatcat tgacttgggt
    23041 gtggattttg agatcatcaa aaaaagcggc tcatggttta gttacggtga tacaaaactc
    23101 ggacaaggaa gagatactgt gaaaaaacta cttgctgata atcccgagct agcagatgaa
    23161 ttagagagtc aaatcagaga ggttttgaaa tcaaaagagt aaaatttttc tgttacttgc
    23221 tatgtcgcgt attataagct aattcgcaca gagttttttt atagtctttt taggtcagat
    23281 actccagctt gttagaccgc gttcagtaaa ctcataacgt ttcacttggc cgccaaaggc
    23341 ctctacggct tttattacct ccgaccttga attacccggg caataaaaaa tcataaaacc
    23401 gccaccaccg gcgccagata ttttgcctcc cgaagcgcca gccaacatag ctgattcata
    23461 aatcttgtca attaaaggat tggtaatacc ttcagccatt tgctttttaa acctccaacc
    23521 ataatcaagt atttcaccaa tttcttccaa ttctccttta agaacagcct ctttcatcat
    23581 cactgcttgc tttttgagct ggtgcatagc ttctattgat tgtttgttct gcgaaaagac
    23641 attttgacgt tgtgtttcaa taattttaga agagagcctg ctggtgcctg taaaataaag
    23701 tattaggttg tgggataatt cattaaggaa tttacttcta attcgtaaag gattgactat
    23761 aactttttca tctttgtaaa attccataaa atttactcct ccaaatgttg ctgcatattg
    23821 atcttgtttt ccgccagcca tcttcagatc attgcgctca atctgataag caagatgcgc
    23881 aatatcatat tcaccaagtg gaagtttgag ccattctgca aaagcgccta aaatagttac
    23941 tgcaagtgtt gacgaagttc ccaatcctga accaggaggt gcgtcaacga atgttgtaag
    24001 tttatatgaa agatttttta atccaaaatc ttttgttaca cgattgtgaa cgcctttgtg
    24061 cagactgagc ttgccgtcca acgaaatctc atttgtactg ttgaattcga caagttcatt
    24121 attatctatc gagttgataa tgattttatt gccattaaga ggttcaatgg tggcataggc
    24181 atacatgctg attgtggcat ttaatatggc gccgccataa atgtcggaat agggggcaac
    24241 atcagtaccg cctccggcca gtcctattct taatggtgat ttactacgat atatcatttt
    24301 gatcagtttt tctaattaaa tgttcaatta cttcaaccat tcgttcccac gaatatttta
    24361 acttttcaac ttttatttgc ttagtaaaac tctcctcttt gttttcctga taaaaccgct
    24421 cgatagcttc tgaaatttct gctgtatcag gattgacaac aaaacccact ttgtcgtccg
    24481 ggacgatctc agccaaacca ccgacattag tagttatcat gggtttttca aaatgataag
    24541 ctatttgagt tactccgctc tgggttgcat ctttgtatgg ctgaactaca atatcgcaac
    24601 agttaaaata atcggcaacc tggttatcag gtataaattc atttgccata atcacctgat
    24661 cagccaattg attttcagta ataagttgaa aatatggttt tggatcacta taaaactctc
    24721 cggctactat cagcttcaga tttcgttttc ttatcctttc atcagccatt gctttcagta
    24781 gtaaatcgag gcctttataa tctctgataa acccgaaaaa aagtaaatat ctgctttcct
    24841 gatcaagccc taatctctct tttgctatgg ttttgctttg taactcgccg aaatgatcat
    24901 aaagcggatg ggggcagaaa agccgcggtc tctttctatc aaaaatgtca agatcgccca
    24961 ttacctcttt tgacatagca ataaaaccat caacagacct gacaaaatat cctgaaagca
    25021 ttttatctcc gatccgcttt tcgtgaggga ttatgttatc taatattgaa atcacttttg
    25081 ttttcctatt ttttcgagct attcgtgcaa ttgttcctaa acaaggagcc ataaaaggga
    25141 tccaatattt gatgatcagg atatctgggt ttaatcgttt tattctcctg ccaattttta
    25201 tccaattaag gggattgaca gaatttactg atatctcaat atcgaagttt tccggttttg
    25261 gttcggtgga atattgtgtt ttccctggaa aaagaaaatt cggatattgt aaagagaatg
    25321 tgattatttt tacattataa tcttgttcaa gaagcgcttg agctaaacgt tggttataag
    25381 ctgcaagccc gccccgtaat ggatgagcgg agccgaggat aatcacattt ttaacgtgtg
    25441 ttttttcact cattggtaat tccggaagtt ttctctattt catactcatt tcgacgtggt
    25501 gaattgcgag agatcatttc agcaacaaaa ccggcagaaa aaacttgcac tcccataatc
    25561 atcgctaaca aaccaaaata aaacaaaggc ctttcggtca ttcgataacc ttgaaggaaa
    25621 aacttggcat aggtaagata aatagcaatg atcaatccta cgataaataa aagtgagccg
    25681 attacgccaa aaaaatgcat cggtcgttta ccgaatttcg atacaaaggt tagggaaatt
    25741 agatcaagaa agccttttat catcctttcg gcgccgaact ttgagctccc atatttacgc
    25801 ggtttatgct gcacaacctt ttctgtaatt tttctgaaac caccccattt ggcaatgaca
    25861 ggaatatatc ggtgcatctc gccataaatc tcgatacttt taatcacatc ttttcgataa
    25921 gctttaagtc cgcaattaaa atcgtgtagt ggaagttttg aaattctacg taccgtccaa
    25981 ttgaaaaatt ttgatggcca gcgtttgcta atagggtcgt aacgcttttt tttccagccc
    26041 gaaacaatat caaacccctt ctcatttatc attttataaa gatcgggtat ttcgtcaggg
    26101 ctatcctgta aatcggcatc catagtaatc actacttccc ctttagcaag aaaaaaacca
    26161 cggtttagag cagccgattt tccataattc tttctgaatc gtacaccctt gatattagga
    26221 tttttatgat tgagcatttc aatcacctcc catgaattat cattgctgcc atcgtcaaca
    26281 aaaatgattt cataggtcag ccccatctta ttaatcacac cttcaatcca tgttgaaagc
    26341 tctggtaacg actcctcctc gttgtataat gggataacaa gcgataaatc cataattatt
    26401 tgttattcgc taaaagcagg tttttccttt ttgataaaaa aagagagaat tgctccaagg
    26461 ataaccccta aaatgatatt tccaaaaaaa ccggttaatg ccattatttt aggactcaca
    26521 aacattttgc tccacttgag agccatctca acttgttctt cactcatatt cggttgactt
    26581 ttataaagat tctcctcggt agcttcgatt atctttgcaa cttcgccggg agctatataa
    26641 ttgtaaaata caaaagtcca gatagctgtt agaattccag taattaaaat tacataaaat
    26701 ccatgtttaa aagcatttcc ataagttata aacccgttat taaacttatc tctccattgt
    26761 ttcatgctta aaaacatacc aataacaatg attagatagg ttaagtagtt gagtgagctt
    26821 ttaggccctg ttcctgccac ataaagaatt agtgaaaaaa tgattaacgc cagcccaagt
    26881 aacagccctt ggttgatagc aactctaaaa gatgagggtt taatttcttc cattatttta
    26941 taatttaagg tttgttgaaa aagtaaggat ttattgtgat gaaatttagt tttttgaccg
    27001 gctgaggcat aaaaaaaatc taccccacct gtttttgaac cattttgcaa aagaaaacaa
    27061 aaaaatctta atgaacttaa tgagtttcat cgaaaacata atgcaaacgc tccaactctc
    27121 actacttttc gctgtgtgaa ttcgtgccct gttgtaagct gttttttctt gtccaaattt
    27181 accttcaata ttatttccaa ttgtctgctc ttcttttgtt tcgatggctt cgcgctcatc
    27241 ccatgggatg atttgggaaa gtttaaccaa acgattgtac ctgtatagat taatctcaaa
    27301 aggggtttta aacttctcaa ttgttaactg gtcggctgat gtgtacttta tcataggtac
    27361 aagctttata atataaaaat acaaactcct tgcattttta tagttaaaaa acatatcata
    27421 agttattaat attcagttga ctaaagcttt tcatcaagcc ctaatttatt gttaaacgta
    27481 agcaaaggta caaatgatac gacgattgga ggtgaaaatt tagaaattaa ggttcatgat
    27541 agcataaatt attatttttg ctgacagggt aagtcctgta cgaccagctc ctgctgaatt
    27601 cccccagggt cggaagacag caagggtagg cggttgtagc ggtgcgatac aggtagctta
    27661 ccctttttta tctccggtga tcagtatgaa aaaatcaaag attttacaat cccaaattag
    27721 ggatgcgaaa tgatatgggt aatattcaca agttgatcat aacgggtgcc ctttgggcga
    27781 taatatacat aaaaggtata ttcattgttg gtttcataaa agctaccttc gataagagta
    27841 acatctccaa cttgtgaatt atttggtaga aagatatact ggtaattata aaagccttgt
    27901 tttaacaaaa ttgaagattc aaaacctcta cgttcaaaat tgtaggtaag ctcattctct
    27961 ttgagaaact gccattgagt aagtccgccc attaggtata tttttccttc t
//
LOCUS       DAOOAI010000014        27871 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_2238_length_27871_cov_4.581823, whole genome shotgun sequence.
ACCESSION   DAOOAI010000014 DAOOAI010000000
VERSION     DAOOAI010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 27871)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27871)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27871
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2238_length_27871_cov_4.581823"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1642
                     /locus_tag="PK345_02075"
     CDS             <1..1642
                     /locus_tag="PK345_02075"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004359307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AlwI family type II restriction endonuclease"
                     /protein_id="HPX33839.1"
                     /translation="SPRTPEKMIPEIQLLNTHFADKEWNNENQIAFMELLRDEYFFFG
                     KGAKDPAFSARDRINRAPKALGFVVLSPKIALTEAGKQLVYSKRKEEVFLRQLLKFQV
                     PSPFHIPTDKATNFWVKPYLELFRLIRHFGSLKFDELMIFGLQLVDYRDFYKIVEKID
                     AFRILKAKNEGNYKKFRVEYLEKELRLIYKDEIKEGDTKIRETKDKSINTFLKTKASN
                     MRDYADACFRYLRATGMVNVSHIGKSLAIVPEKIIDVDYFLENTKREPQFYDNESQYI
                     NYLGNASIPTLYTDNREHLEQKIKTEFPQLKISSKNSLLELKEIYADNLERRKEQILS
                     KQTKAIKDYRLFGDINTVFEQIIDSSIYDAPLMLEWNTWRAMTMLDGGNIKANLKFDD
                     FGNPMATAQGNMADIVCDYDDFGVLVEVTMQCGQRQYETEGEPVTRHLAKFKREIKKP
                     AYCLFIAPKINESVIAHFYGLHKLNISYYGGSSTIIPLPLNVFLKMIEDSHKASYVPE
                     PKHVRAFFEYSNELVNLVKSEIEWYHGITEKALNWLPK"
     gene            complement(1823..3178)
                     /gene="purB"
                     /locus_tag="PK345_02080"
     CDS             complement(1823..3178)
                     /gene="purB"
                     /locus_tag="PK345_02080"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0006188 - IMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HPX33840.1"
                     /translation="MLSCDQLRAISPIDGRYHNKTMMLQEYFSEAALFSYRIQVEVEY
                     FISLCNIPLPQLSDVKPLVIDDLRNIYINFTIEDARQVKKIEKITNHDVKAIEYFLKE
                     KFDLMGLRKYREFIHFGLTSQDINNTAIPLAIKEAWKKLLSDQVVGMVKVLKGLAYEW
                     KNIPMLAHTHGQPASPTLLGKEIMVFATRLEQQLKLVNEIPFSGKFGGATGNFNAHVA
                     AYPEINWAKFADQFLEKSLGLKRQQPTTQIEHYDNLAAFFDGLKRINNILIDLARDMW
                     SYISMDYFKQKIKKGEVGSSAMPHKINPIDFENAEGNLGMANAIFEHLSAKLPISRLQ
                     RDLTDSTVSRNIGVPLAHSLISWRSLVKGLDKLIVNTEKINDDLDQNWEVIAEAIQTI
                     LRRENYPKPYEALKEFTRKNKKIHKEEIHQFIDSLNISGKLKDELKMLSPFTFTGINP
                     F"
     gene            3616..4800
                     /locus_tag="PK345_02085"
     CDS             3616..4800
                     /locus_tag="PK345_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPX33841.1"
                     /translation="MKNSENLGFFTRQVHAGDFNDPLGSATVPIYQTSTFKFKNADHG
                     AACFAGEQKGYIYTRLGNPTIAALENAVANLEKGFAGIATSSGMAAITTVYLTFLGKG
                     KHIVSHNALYGPARSVMETIFARFGVEYTYVDTTNIENVKSAIRPETAMIYTETPANP
                     TMGISDLKKLADLAHEIGVPLVVDNTFCSPYLQRPIEFGADVVVHSMTKFLNGHADIV
                     AGMIITANEKYHKLINPVMINMGCNIDPHQAYMVHRGLKTLGLRIERAQASAMKIAEY
                     LQNHPKVAKVNYPGLKSHSQYELAQKQMYGPGTMMAFELKGGFNAGKKLIDNVQLILL
                     AVSLGGIESLIQHPASMTHSKVSHEKKIEAGITDELVRLSVGIENVEDLIADLDQAFE
                     KV"
     gene            4918..7218
                     /locus_tag="PK345_02090"
     CDS             4918..7218
                     /locus_tag="PK345_02090"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HPX33842.1"
                     /translation="MFSAFYRKFLVFSLMLIFITPLCYGQKVAVVLSGGGSKGAAHAG
                     VLKALEENNIQIDYIIGTSIGAFVGALYASGYSPEEIENFFISTDLKRWSSGESNPKF
                     NYFFKMDEPDPSWVNFDFNFKTPLTKILPTSLVPTTEMDFTLMSLFAEASAASNQNFD
                     SLFIPFRCIAADIDSDKALTLSNGDLGAAVRASITFPLFFKPIKIDDRLLFDGGMYNN
                     FPHDVAMDVFKPDLIIGSNVSKNYPSPDPDDVVSQLQKMLMTDADFNLSKESGIIIQP
                     KVSRPNLTDFSLSQEFIQLGYDETIRRMPEIKERIKDFRKDGETNELRNKFKAKFQPL
                     FIDSISNAGLTKNESAYFKKQLFHEYNTITLAEVETGYYNIATDGYFKVGMPLLKPNA
                     TRQKYTLDLGLKRSDRFNVKFGGNLSSRLANVGFVELNYKHLSSQGFKLKTNVFVGRF
                     HSSVLLGFKLESPSKPPFYLDANLVYNHFDFFKSTIHFFEDITPSYLISNENYVRVTA
                     GAPTNIKGKFEANITLGSMDNNYYQSNMFTRLDTADKTTFNFSKAAVRWELNTLNRKQ
                     YANAGARFLLEATLINGKENFYSGSLTYERSDLKDIKHKWVSVGMKWENYFTKPKKLK
                     FGFYSEVSLTTQRFFSNYTSSLLMAPAFFKVPESQTIFLPNYRAFSYGVGGLITIFTI
                     AKNFDFRFENYIFQPYRQIFSDKNYKSFFGEKFKNRYLMSSAVLVYNSKLTPISISLN
                     YFDNPEEKFFFSFNIGYLIFNKRALY"
     gene            7229..9772
                     /locus_tag="PK345_02095"
     CDS             7229..9772
                     /locus_tag="PK345_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPX33843.1"
                     /translation="MRPLITILFSIIVLITYGQEQTVNVRSIETYFAELGEVCFSFDL
                     ENDDMMRKLTAIISIDNVVGKTVMAYANQEEFNQFLEFRIPYKIVPHSSDIPVEITML
                     GKDNLKDITDWNFYPTYDAYISMMYQFAIDYPDLCNVFSIGNSVQGRQLVMAKISKNV
                     NTREPEPQFLYTGTMHGDELAGYNFLLRMIDYLLTNYSVDPDVTYLLDNVEIWVNPLS
                     NPDGTYKGGNNTVNSSVRYNANNVDLNRNYPDPEDGPHPDGKAWQPETIAFMQLAEQN
                     NFVMAGNTHGGEEVINYPWDTWSNLTADNNWWIFVCREYVDTVHIYSPANYMTYLNNG
                     ITRGYSWYSISGGRQDYMNYFHNCREVTMELSKVKRLATSSLNTYWNYNYRSMINYIK
                     QVTYGVAGTVTDLLSNEPVRAKITINGHDTDNSHVFSDSQNGFYQRLLDSGTYNITFS
                     SPGYLPLTVEGVNVQKYQKTVLNVNLDPGPLNPVISSSARAIPIESTVTFTDESGGKP
                     VSWEWAFQGGTPNSSSLKNPEPVLYSTAGTFDVKLSITNSNGEKITRVFEDYIRVAPI
                     ELMSNKSVTTCNSLFYDSGGENGSYGNNQMLTLTFLPGLSGAMVEADFLEFGLEASSG
                     CIYDYLRIFNGSNTEAPLIGVWCGANSPGKISATNPAGSLTFQFKSDAYVTSTGWKAL
                     ISCKTTEEIIIENGWSGISSYVVPIDTNIDTLFQSIIQDIVVVIGDDGIYSPELNIFT
                     LQDWNSRQAYLVKSTSPQLLPMTGTLMLNRNISLNQGWNLVHIPTPQQIKTSSIQSIL
                     NDDLIMIKPVADIHLFWPQMGINSLTNLLPGKAYYFNVSKKVEFMFPDK"
     gene            9840..10310
                     /locus_tag="PK345_02100"
     CDS             9840..10310
                     /locus_tag="PK345_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33844.1"
                     /translation="MRILLTSILLCFAMMLSAQSDIDVVIMKSGVEHKCTIQEIKNDT
                     IVISQFLGRSKVFESIPINEVATYILNNHYSSPGEDLMKATGLFFVATGFMVTGSVVA
                     VMALNADNKDLALAGTAFGLFGSVFLFSGYSKMQSASKKLNKLHFENDRIIYKL"
     gene            complement(10401..12032)
                     /locus_tag="PK345_02105"
     CDS             complement(10401..12032)
                     /locus_tag="PK345_02105"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010265717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HPX33845.1"
                     /translation="MADTKYIFVTGGVTSSLGKGIISVSLAKLLQARGYSITIQKLDP
                     YINVDPGTLDPYEHGECYVTEDGAVTDLDLGHYERFSNVPTSQANNVTTGKIYQAVIN
                     KERKGEFFGSTVQVIPHITDEIKYRIKLLGNSGKYDIVITEIGGTVGDIESLPYIEAV
                     RQMRRELGSNCLVIHLTLVPYLAAAEELKTKPTQHSVKTMLEQGVQPDIIVCRTERPL
                     TNSLKAKIALFCNVDDSSVIESLDANTIYDVPLMMQKERLDLEVLRKLKMTSKHEPNL
                     EGWEEFLHRIKNPTSEVTIGLIGKYVELKDAYKSIFESFIHAGAINNCKVRVKTVHSE
                     HIEENGNGNLMEGFDGILVAPGFGPRGVEGKIIAVNYARLQRIPFFGICLGMQCAVIE
                     FARNVLGLKKANSLEFNQKTPWPVIDMMEDQKKIAGLDGTMRLGKFDCFLKPNSKAFQ
                     IYGTQNISERHRHRYEFNNKFSEQFETAGMKQSGINLQTELVEIIELENHPWFIGVQF
                     HPEYKSTVANPHPLFVSFVKSAMEYSSSKSLTNKK"
     gene            complement(12299..13699)
                     /gene="gltA"
                     /locus_tag="PK345_02110"
     CDS             complement(12299..13699)
                     /gene="gltA"
                     /locus_tag="PK345_02110"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004355 - glutamate synthase (NADPH)
                     activity [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent glutamate synthase"
                     /protein_id="HPX33846.1"
                     /translation="MAIKKNRVPVRSQLPEERITNFDEVCFGYNASEAIQEAERCIQC
                     KNPRCVQACPVYIKIPQFIKAVAESRFGDAVDIIAEDSSLPAVCGRVCPQEVQCEGSC
                     VMGIKGEPISIGKLERFVADWGRTTNYYKPTTVEQNSYKVAVIGSGPAGLSCATDLAK
                     MGYQVTIFEALHRPGGVLEYGIPEFRLPKERVVRYEIDNVVRLGVNIETDVIVGRTIT
                     IDELLDTEGYHAVFIGSGAGLPKFMNIPGENLNGVVSANEFLTRTNLMKAFDPEYDTP
                     VYVGKRVVVVGGGNVAMDAARTACRLGAEVYLVYRRSEKELPARLEEVHHAKEENIIF
                     RLLNNPTEILGDENGWVKAIRCIKMELGEPDDSGRRRPIEIKGSEFEIECDVVIMSLG
                     TSPNPLIAKTTPGLKTESWGGIVANEETGETSREGVFAGGDAVTGAATVILAMGAGRK
                     AAKQIDTYIRQKAEKE"
     gene            complement(13705..14577)
                     /locus_tag="PK345_02115"
     CDS             complement(13705..14577)
                     /locus_tag="PK345_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010264663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfide/dihydroorotate dehydrogenase-like
                     FAD/NAD-binding protein"
                     /protein_id="HPX33847.1"
                     /translation="MFTIVKKRLLAPNIYLMEVNAPRVAKSAQPGQFVIVRVREKGER
                     IPLTIADFNRQDGAVTIVTQSIGASTKLICALQEGEALLDFAGPLGQPSELIHMTDEE
                     LANTRILFIGGGVGTAPVYPQVKYLSERGFKADVIIGAKNKDYVILEDEMRAVVNGYY
                     LTTDDGSASFKGLVTDRLKDLIENQGKKYDLVVTIGPMIMMKFVTMLTKEYGIKTIAS
                     LNTLMVDGTGMCGACRVSIGGKTRFTCVDGPEFDAHLVDFDEAMRRQNMYRTIEGQKI
                     AELELENHKCKIGL"
     gene            14959..15468
                     /locus_tag="PK345_02120"
     CDS             14959..15468
                     /locus_tag="PK345_02120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33848.1"
                     /translation="MNNFEIISLILYLFLFVSMMVIIFFGIDFLRNKKASQSETIRII
                     TPLRLQAYERIILYLERISPQVIATRLLNPVLTAREFQNTIIQSINEEFNHNYTQQLY
                     VSDETWKKVLLVKENVIRSVKIAFATLPEKATASDLASRLLEDKEQQVQKEISQAIAY
                     LKKEAQSLF"
     gene            complement(15453..15953)
                     /locus_tag="PK345_02125"
     CDS             complement(15453..15953)
                     /locus_tag="PK345_02125"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPX33849.1"
                     /translation="MTNHVLEFLKTLVGKDLKSSPSPIGVWLNGTLVSIEEGKACIRY
                     IVRKEMTNPLGTLQGGIFTTMMDDALGVAVYSLGEDKLYVTINFYVDFLESVHENDAI
                     TIEASVLRKGKSLLNVEIKAINDLNKIVAHGKSNLAVRTVDGLVLPKYEGMEKKYPGL
                     TFQNKD"
     gene            complement(15977..17773)
                     /locus_tag="PK345_02130"
     CDS             complement(15977..17773)
                     /locus_tag="PK345_02130"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HPX33850.1"
                     /translation="MEITRIFDLLDHLKNNYPSDAILAAKKNGNWIKYSTDDYIRYSN
                     LFSIGLLALGFEKGDKIATVTNNRPEWNFIDMGMLQVGIVHVPLYPTISLDEYKYILT
                     HSDVKAVIISDKQLYAKLEKLIGTIPAITKVYIFNEVEGVANWNEIIALGEQNEAHFK
                     TRFNEIKDTIKPEDLATIIYTSGTTGTAKGVMLSHRNLVTNFISTSKIQPLKYGHKVL
                     SFLPLCHIFERMMNYHYQYLGISIYYAENMGTIAADLTEIKADGFTAVPRVLEKIYGK
                     IVAQGKDLPWLKKEIFQWALKLGSRYELNGQNGKFYELKLKIADKLVFSKWRAAIGGR
                     IQVVVSGGAALQPLLSRIFWAADIKVVEGYGLTETSPVIAVGYPFWPKIKFGTVGPIL
                     EGVEVKFDEEGEILVKGPNVMMGYYKDPEYTKQVFDEEGWFHTGDIGMMVDGEFLKIT
                     DRKKEIFKLSSGKYVAPQLIENKFKESIFIEQIMVVGENEKFASALISPNFNYLHFWA
                     SKYKIHYRDNEELIKKTLVIERIQREVDLVNKELGDHEKIKRFRLVCEEWSPQSGELS
                     PTLKLRRNIIYKKYDHILREIFQYSKNDENGK"
     gene            17996..19489
                     /locus_tag="PK345_02135"
     CDS             17996..19489
                     /locus_tag="PK345_02135"
                     /inference="COORDINATES: protein motif:HMM:NF040164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5723 family protein"
                     /protein_id="HPX33851.1"
                     /translation="MIIKINSGFTTKLGILTILLINITLNQLRAQERYGITLSNFGGI
                     NAATINPSFTVNSKTFLDVNFLTAGLTIENNFVFYHKNDFKFSELLRANPNLPTNEVR
                     GEGLDYSLKYQNLTGLAYVEALGPSFSLNIGKHAGGLFTRTVAASSFVNVPQDLGVLL
                     FEGIRYKPLYGKEISGQKINVDALGWSEIGLNYSYNYLSGYYNNWSVGINIKQLLGYS
                     GAYLSADDLKLNVVNRDTVDITNILANIGYSLPINYGSNGYSRTKPLFLGKGIGLDFG
                     FTFRRNLDFAPMGRVTRYCESYFQPYRYKLGVAVSGLGNIHFNKNTREHTFENRSALW
                     ENIDTTEFRNINDFFENLSSVFYGDPHSLPTEEDNMIVGLPTVLNLNADYQYFPNWYL
                     SGMLVLPVKSSDYQLMFPKKALLGIRYETDDLEFNFSSSFFNFQKLHIGFFVRYKYFS
                     IGTSKLESLVKATNLYGINLYFSIKFHLIKGYCLSLPPKHDCGRFQF"
     gene            19640..19715
                     /locus_tag="PK345_02140"
     tRNA            19640..19715
                     /locus_tag="PK345_02140"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:19675..19677,aa:His,seq:gtg)
     gene            complement(19867..20883)
                     /locus_tag="PK345_02145"
     CDS             complement(19867..20883)
                     /locus_tag="PK345_02145"
                     /inference="COORDINATES: protein motif:HMM:NF027451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4837 family protein"
                     /protein_id="HPX33852.1"
                     /translation="MKVLFKLWPCALFCWFAFLLAACSSNPSGHKPSTGKTNELLVVV
                     NNETLHKGHVGSVISEFFGQILTGLPQPEKTFDVAFIYEPGFGQMFKTHHNIFIVDIN
                     ASFDKPVIETHSDLWAKPQRIIKMTVHDEATFFEEFEEKKESFMMYFNVNERRRAAQA
                     FGSIEDLKIKKFITKKFDLNILIPKNFYVAKEQQNFVWIRREGQEFSQGILIYTIPYH
                     DTSVFLPDEIIKLRDRMTKDNVLGPSEGSFMRVSMVEPPESHRIEFNGMFAVEMRGMW
                     ELDGDFMGGPFINYTVLDEQNQRIVTVDGFVYYPNKDKKNLTRQLESLIYTLSFINPD
                     QEKQ"
     gene            complement(20903..22699)
                     /locus_tag="PK345_02150"
     CDS             complement(20903..22699)
                     /locus_tag="PK345_02150"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPX33853.1"
                     /translation="MSQTSYGKNKPKFDQDRKSHLFEAEDFEDSALFETPDFSSDTNT
                     LIESFDFADSTFIFSNSKMEDPRITQELLNESRALYMLDNLTTLKFFRDDETLFDKNK
                     LNIYNYDEAFVPKFSDSVYQSRIEIMNRKTPLEFVYNETVKGYIDVYGIKKRGLSSRI
                     LGLAAIYFPMFEELLDQYNLPLELKYLAVVESALNPLAGSPAGAKGLWQFMYNTGKVY
                     GLELNSYVDDRYDPYKSTIAACKHLKDLYDIYEDWWLVLAAYNSGAGNVNKAIRRSGG
                     IKNYWAIWPFLPRETRGYVPAFIAVAYVMTHSAEHNLYPTHPGILYNAIDTVQVYDVL
                     TFDRISEMIGISKENLKYLNPSFKEGVIPATKDNKYILRLPRTLTAYYVSKEEEIYKY
                     RTETELEKEKLMAKIKEMPKYQIHIVKRGETLGGISRKYKTTVSTIQALNNLRGTTIH
                     PGQKLYVSGVPPTNIGKSSAATASSNNTNNTIHVVKKGENLGLIASKYKCSVNDLKNW
                     NNLVSNTIHVNQKLIVSKSAEAVAGTASQENQSTSESDGYIYYIVKPGDTLWEIAKQY
                     NGVTVEGLKKLNNIKNTKALKVGQKIKITTAG"
     gene            complement(23274..23759)
                     /locus_tag="PK345_02155"
     CDS             complement(23274..23759)
                     /locus_tag="PK345_02155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33854.1"
                     /translation="MKGLTLEIKPLEGFGELEFGSTPDEAIRYLGEPTEEEFIEEDDD
                     FDESMIQYFDDHGISIFYEEVDGLFLSNVETENPETILFGAKVFELTIDEVINLMKEN
                     GYSDYEIEEIDLEDNEEEKGQWLIFDDALIDFLFEEGKLSNINWGAPLDDDDYYDDDE
                     D"
     gene            complement(23784..24605)
                     /locus_tag="PK345_02160"
     CDS             complement(23784..24605)
                     /locus_tag="PK345_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPX33855.1"
                     /translation="MTKRFDNKVVVITGASSGIGKALVYEFARRGAKIVAAARSADQL
                     SAMQHEVNELFKSEVLAVPTDVGIEKDCKNLIEQTIDKFGKIDILINNAGVSMRALFN
                     DLHLDVIRKLMDVNFWGTAYCSKYALPFLLSSKGSLVGVSSIAGYVGLPARSGYSASK
                     SAMQGLLGVIRTENLKQGLHVLIACPGFTASNIRFKALTSDGSQQNETPRNEEKMMSA
                     EKVAKKIADAIVNRKRSLVLTTEGKLSILLNKFIPATVDKLVFKKMSKEINSPFK"
     gene            complement(24732..27626)
                     /locus_tag="PK345_02165"
     CDS             complement(24732..27626)
                     /locus_tag="PK345_02165"
                     /inference="COORDINATES: protein motif:HMM:NF024893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA-like C-terminal region-containing protein"
                     /protein_id="HPX33856.1"
                     /translation="MKKFFKISGIIVALILLLMIIVPYIFKDKIVAVVKNEINKNINA
                     NVDFNRVGLSLFRNFPDISVRFTELSVINNEPFEGDTLAYIPTLHATIDIMSVLRGDD
                     IQIKKVNLNNSFINLLVKSDGRANWEIMKETDETDIEATPEEESSSLLIKLKKFTITN
                     SKFVYDDKSLAFYLLLENVNHELSGDLTLDNTTLQTHTTAEKLTASYEGIRVLQEVDA
                     ELDSPLDIDFNKFLFTFNNSELRLNQFFLLFEGTFEMPDNGDYVMDFTYSSKRTDFKD
                     FLSLIPVMYATDLKNISTAGNAIINGNLKGIYNDDSYPGFEFNALINNARFHYPDLPK
                     SVDEININASIKYPGGIDFDKIEVDVPNLSLFLGGNKLSMELFVRNPVSDLALKGKID
                     GMINLSEIGDFYPLPNGENPQGKITANISLDGKMSTIEKRQYEAFNFDGSLLLDSFIM
                     RSASIDKTIEIAKAQLSFSPRFVNLLHLDLKVDNNDLSANGKLENFIPYLLSDGVLKG
                     NLQLNSNNFNITDLLPKTENSDLPDDTVAISPVKIPDNIEFSVQALFKKLIFDNIELT
                     NTTGEMLIVDQELILKRLNMEVLGGTMFLNGKYDTRDSTQAKAELALNLNQIDIQKSY
                     STFGTINKFAPIAQKTDGKFNTSLNIKTLLDNQMMPVYSSMNGGGNLKTTNIVIENFS
                     IANKIADLLKMPDLKRLLMGPVNLSFEFVNGKVHLKPFELSYQDINANTLGWISFDQT
                     IDFEMAITIPRTKFGGAANSVLENLVNEANKLGTSFKLGEKVNITAKITGTTSDPVVK
                     IMPGNGSGKSMLDDMKKRAQEELDKQKQKLEDEARKELEKRKAEAKAQSDKILADADK
                     QAAQIINEAQKQADAINRAAQEAADKLKAEAQKQSENMIADAKKKGPIAEAAAKKAAE
                     QLKKETDKKAEKLIAEAKNNSDKLVSEAQRQSEKIRSDARNQVDKLMDIK"
     gene            complement(27637..>27871)
                     /locus_tag="PK345_02170"
     CDS             complement(27637..>27871)
                     /locus_tag="PK345_02170"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018360264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPX33857.1"
                     /translation="TIWFRVAFKPVPSIKKAQLTIDYHGNKKEISISGRHDVCVIPRV
                     VPVVEAMAALVIADHFLRNIHVHHFVNKIHPKI"
BASE COUNT         8655 a         5426 c         4842 g         8948 t
ORIGIN      
        1 ctctccaaga actccggaaa aaatgattcc ggaaattcaa ttattaaaca ctcactttgc
       61 agacaaagaa tggaataatg aaaaccaaat cgcttttatg gaattactta gagatgagta
      121 cttctttttt ggcaagggag caaaagatcc tgcatttagt gcaagagata gaattaaccg
      181 tgctcctaaa gccttaggat ttgttgtact atctcccaaa attgcattaa ctgaagctgg
      241 gaaacaatta gtctactcaa aaaggaaaga agaggtattt ttacgtcaat tacttaaatt
      301 tcaagttcca tctccttttc atattcccac tgataaggcc acaaactttt gggtaaagcc
      361 ttatcttgaa ttgtttagac taattcgtca ttttggttct ttaaagtttg atgaattaat
      421 gatttttgga cttcaattgg ttgattatcg agatttttat aaaattgtcg aaaaaattga
      481 tgctttcaga attttaaaag cgaaaaacga agggaattat aagaagttta gagttgaata
      541 tttagaaaaa gaacttagat tgatctataa agatgaaata aaagaaggcg atactaagat
      601 cagagaaacg aaagacaaat caattaacac atttttaaaa accaaagcaa gtaatatgcg
      661 tgattatgct gacgcttgct tcagatatct tcgagccaca ggaatggtta atgtatctca
      721 tatcggtaaa tctttggcta ttgtacctga aaaaattatc gatgtagatt actttctaga
      781 aaatactaaa agagaacctc aattttatga taatgaaagt cagtacatca attatctcgg
      841 aaatgcttcc atacctacgc tatatacaga taatagagaa cacttagaac aaaaaatcaa
      901 gacagagttt ccacagctaa aaatctcttc aaaaaatagt ttattagaat taaaagaaat
      961 ttatgccgat aatttggaga gaagaaaaga gcaaattctt tctaaacaaa ctaaagcaat
     1021 aaaagattat agattatttg gtgatattaa tacagttttt gaacaaataa ttgacagttc
     1081 tatatatgat gcacctctaa tgctcgaatg gaatacttgg agagctatga ctatgttaga
     1141 tggcgggaat attaaggcta atctgaaatt tgatgatttt ggaaatccga tggctacagc
     1201 gcaaggaaat atggctgata tagtttgcga ttatgatgat tttggggtgt tagtagaagt
     1261 aacgatgcaa tgtggacaaa ggcaatatga aacagaggga gaacctgtta caagacatct
     1321 tgctaagttt aaaagagaaa taaaaaaacc agcttattgc ctttttatcg ctcctaaaat
     1381 aaacgaatct gtaattgcac atttttatgg attgcataaa ctgaatatca gttattatgg
     1441 aggaagttct acaataattc ctttaccttt aaatgtattt ttgaaaatga tcgaagattc
     1501 tcataaagca agctatgttc ctgaaccaaa acacgtccga gcattttttg aatattcaaa
     1561 tgagttagtc aatttagtaa aatcagaaat agaatggtac cacggtataa ctgagaaagc
     1621 tttaaattgg ttgccaaaat aatgggcgat caactaacaa attgttttgc ataaactgca
     1681 gagaaagtct ctgattgaaa taacttgcta tatttttaag ttcggtcttc gcttaaaaat
     1741 ttcgactaaa aataagcttc ctcttaagct accttaatgt tttgcgtcca acaacaaaaa
     1801 tcctgatcag aagccctcta agttagaatg ggttaatacc tgtaaaagta aatggcgata
     1861 gcatttttaa ctcatctttt aacttgcctg aaatatttaa cgagtcaata aattgatgaa
     1921 tctcctcttt gtggattttc ttattttttc gggtaaattc ttttaaagct tcatagggtt
     1981 tcggataatt ttcccttctg agtattgttt gaatagcttc tgctatcacc tcccagtttt
     2041 gatcgagatc atcatttatt ttttcagtgt taacgatcaa cttatcaaga ccttttacta
     2101 atgacctcca tgaaataagc gaatgtgcca aaggaactcc aatgttgcga cttactgtgg
     2161 aatcggtaag atcgcgctgc aaacgggaaa tgggtaactt agccgaaaga tgttcaaata
     2221 tggcattagc cataccaaga ttaccttctg cattttcaaa atctatcggg ttaattttgt
     2281 gaggcatagc agacgaacca acctccccct ttttgatctt ctgcttaaaa taatccatcg
     2341 agatataact ccacatatca cgcgcgagat caataaggat attgttaatt ctcttcagcc
     2401 catcaaaaaa tgctgcaaga ttatcgtaat gctctatttg tgtagtaggt tgctgtcttt
     2461 ttaaaccgag agatttttct aaaaactgat cggcaaactt cgcccagtta atttctggat
     2521 atgcagcaac gtgagcatta aaattacccg tagctcctcc aaactttcct gagaatggga
     2581 tttcatttac taatttcagc tgttgttcca aacgagtagc aaataccata atctctttac
     2641 ctaacaatgt tggcgaggcc ggctggccat gcgtatgtgc aagcatcggg atatttttcc
     2701 attcgtatgc aagccctttt aataccttaa ccataccaac aacctgatcg gaaagcaatt
     2761 ttttccatgc ctcctttatt gccaagggaa ttgccgtatt attgatatcc tgcgaggtaa
     2821 ggccgaaatg gataaattcg cggtacttcc ttaaacccat caaatcaaac ttttccttca
     2881 aaaaatattc aatagctttt acgtcatgat ttgtaatctt ttctatcttt tttacttgcc
     2941 tggcatcttc aatagtaaag ttgatataga tatttctaag atcgtcaatt accaatggtt
     3001 taacatccga gagttgtggt aaaggaatgt tacataagct aataaagtac tcaacctcta
     3061 cctgaatcct gtaactaaac aatgccgctt cggaaaaata ctcctgcagc atcatggttt
     3121 tattatgata tctgccatct attggcgaaa tagcacgtaa ttgatcacaa cttaacatga
     3181 tagtttcttt aattttataa atcaaaaaga ataatgaact tgtaaagatt agccctattt
     3241 aaatagcaat caatggatta caaaagccat attgcccttt ttgttgaata tcgcagagct
     3301 aattttttta caaatgtaaa cgaaaagtaa tacgtatcac tataacctaa ttttttttgt
     3361 ttttttttaa aaggataaag gatactaaac ccagattaat caacagaaga cctcaatggc
     3421 aaaactattg atttattaag gctaatcata atttattatt ggtttactgc aactcttatt
     3481 gtgctgatta taaatgctta atatgagatg aatactttac aatctcttac cacccttact
     3541 gcaaaccacg atgactaagg ataatatcaa taattttata ggcgaaatta atgatcaaat
     3601 ttttattata ttaatatgaa aaactcagaa aatcttggat ttttcacccg tcaagttcat
     3661 gcaggtgatt ttaacgatcc gttaggaagc gctactgtgc ctatttatca aacatcaaca
     3721 ttcaaattta aaaatgctga ccatggagct gcctgttttg caggtgaaca gaaaggatat
     3781 atttacacac gacttggcaa tcccactatt gcagctcttg aaaatgccgt agctaatctt
     3841 gaaaaaggtt ttgccggtat tgctacttca tcggggatgg ccgcaattac caccgtttat
     3901 ttaacctttc tgggaaaagg taagcacatc gttagccaca atgcgcttta cggaccagca
     3961 agaagtgtca tggagactat ctttgctcgt tttggagtag aatacactta cgttgatact
     4021 accaatatcg aaaatgtaaa aagcgctata aggccggaaa cagcaatgat atacacagag
     4081 accccagcta atcctacgat gggtatttct gatctgaaaa agcttgccga ccttgcgcac
     4141 gagattggtg taccgctggt tgttgacaat accttttgca gcccctatct gcaacgccct
     4201 atcgagttcg gcgctgatgt agttgttcat tcgatgacta aattcctaaa tggccacgct
     4261 gatatcgttg ccgggatgat tattaccgca aatgaaaaat accacaagtt gataaatccc
     4321 gtgatgatta acatgggttg caatattgat cctcatcagg cttacatggt acatcgagga
     4381 ttaaaaactt tagggcttag gattgaaagg gctcaagcat cggcaatgaa gatagccgag
     4441 tatctccaaa atcatccgaa agtagcaaag gtaaactatc ctggtctaaa gtcacattcg
     4501 cagtacgaac tggcacaaaa acaaatgtat gggcccggaa cgatgatggc atttgaatta
     4561 aaaggtggtt ttaatgcagg caagaagctt attgacaatg tacaattgat acttcttgca
     4621 gtgtcgcttg gcggaataga atcattgatt caacatcccg cctcaatgac ccactctaaa
     4681 gtgtcgcatg aaaagaaaat tgaagccgga ataaccgatg agttagtaag actatccgta
     4741 ggaattgaaa atgtagaaga tctgattgcc gacctcgacc aagcctttga aaaggtatag
     4801 atcaaaattt ttctaatatc ctgattgttt tttgacgttc taaaaaaata aaatcaggct
     4861 taatgacaga aatgtctatt tttatatcct gatttttatc aattaaaacc aatccggatg
     4921 ttttcagcct tttacagaaa gtttcttgtt tttagcttga tgttaatttt catcacgcct
     4981 ttgtgctatg gtcaaaaggt ggctgtagta ttgagtggag gcggttcaaa aggtgcagca
     5041 catgctggag tattaaaggc tcttgaagag aacaacatcc aaattgacta tattattgga
     5101 acatctatcg gagcattcgt tggcgctctt tacgcttccg gttactcgcc tgaagagatc
     5161 gagaactttt ttatttctac cgatctaaaa cgttggtcgt caggtgaatc aaacccgaaa
     5221 tttaactatt tttttaagat ggacgaacct gacccttcgt gggtaaattt tgattttaat
     5281 tttaaaacac ccctaaccaa aattctgcct acaagtttgg ttcctactac cgaaatggat
     5341 tttaccttaa tgagcctctt tgcggaagca tcggcggcat caaaccaaaa cttcgacagc
     5401 cttttcatac ctttcagatg tatagctgcc gatattgatt ccgataaagc gctaactttg
     5461 tcaaatggag atttaggtgc agcggtaaga gcttcaatta catttccgct gttttttaaa
     5521 ccaataaaaa ttgatgatcg gctgctgttt gatggcggca tgtataacaa tttcccccac
     5581 gatgtggcaa tggacgtttt caaacctgat ctgataattg gaagcaatgt atcgaaaaac
     5641 tatccaagcc ccgaccctga cgatgtagta agtcagttgc aaaaaatgct gatgacagat
     5701 gccgatttca atttatcaaa agagagtggt ataatcattc aacctaaggt atcaagacct
     5761 aaccttaccg acttttcact ctctcaggaa tttatccaac ttggatatga tgaaacaata
     5821 agacggatgc cggagatcaa ggagagaatt aaagatttca gaaaagatgg agaaacaaat
     5881 gaattacgaa acaaattcaa agcaaaattc caacctctct ttattgatag cataagcaat
     5941 gctggtctta caaaaaatga atcggcatat ttcaaaaaac aattgtttca tgagtataac
     6001 actattactc ttgcagaagt agaaacgggt tattacaata ttgcaaccga tggatatttt
     6061 aaagtaggca tgccactttt aaaacccaat gccacacgcc agaaatatac attggacctg
     6121 gggctgaaaa gaagcgaccg attcaatgtt aaattcggcg gaaatttatc atctcgttta
     6181 gcgaatgtgg ggtttgttga gttaaattat aaacatctct cctcacaggg gtttaaatta
     6241 aaaaccaacg tctttgtcgg tcggtttcat tcatccgtcc tacttggatt taagcttgaa
     6301 tcgccgtcga aacctccgtt ttaccttgat gccaacctgg tttacaatca ttttgatttt
     6361 tttaaaagca ctatccattt ttttgaagac ataactccct cctatcttat ttcaaatgaa
     6421 aattatgtaa gggttactgc aggcgcgcca actaatataa aaggtaaatt cgaagcaaat
     6481 atcaccttag gctcaatgga taataattat tatcagtcta atatgttcac gcgattagat
     6541 actgccgaca aaaccacttt caacttctca aaggcagccg ttcgttggga actaaatact
     6601 ttaaaccgaa aacagtatgc taatgcggga gctagatttt tattagaagc tacactgata
     6661 aacggcaaag agaactttta cagtggtagt ctaacctacg aaagatcgga tttgaaagac
     6721 ataaaacata aatgggtgag cgtgggaatg aaatgggaga attatttcac aaagccaaaa
     6781 aaactgaagt ttggttttta tagtgaagtg agccttacaa ctcaaaggtt tttctctaat
     6841 tacacctcaa gcctgcttat ggctcctgcg tttttcaaag taccggagag ccagactatt
     6901 ttcttgccaa attatcgcgc ttttagctat ggtgtaggag gactgatcac aatatttact
     6961 attgccaaaa attttgattt ccgatttgag aattatatat ttcaaccata tcgccaaatc
     7021 tttagcgata aaaattataa atcgtttttt ggagaaaagt ttaaaaatcg ctatctgatg
     7081 agttcagcag tattggttta caatagtaaa ttaacgccga taagcatttc gctaaattat
     7141 tttgataacc cggaagaaaa atttttcttt tcatttaata ttggttacct gatatttaac
     7201 aaaagagctt tgtattaaat ttatttttat gagaccactt atcaccattt tatttagcat
     7261 aatagtgtta atcacttatg gtcaggagca aacggtaaat gtaagatcaa ttgaaacata
     7321 ttttgccgaa ttaggcgagg tctgtttttc atttgatttg gagaatgatg atatgatgag
     7381 aaagctaaca gctatcatat ccattgacaa tgtcgttgga aaaacagtta tggcttatgc
     7441 aaatcaagaa gagttcaacc aatttcttga atttaggata ccatacaaaa tagttccaca
     7501 ctcatcagat attcctgttg agatcacaat gttaggaaag gataatctga aagatataac
     7561 cgactggaac ttttatccca cttacgatgc ctacatcagt atgatgtatc agtttgccat
     7621 tgactatccc gatctatgta atgtttttag tataggaaac tcggttcagg ggcgacaact
     7681 ggtgatggcc aaaatttcga aaaacgtcaa cacaagagaa ccagagcctc agtttctcta
     7741 taccggaacc atgcatggag atgagcttgc tggctataat tttttactta ggatgattga
     7801 ttacctattg actaattata gcgttgaccc cgatgttaca tatctcttag ataatgtcga
     7861 aatatgggtc aacccgctct ccaaccctga tggaacatac aaaggtggca ataatacagt
     7921 aaatagctca gtgcgttata atgctaataa tgtagatctc aaccgcaact atcccgatcc
     7981 tgaagatggt ccccaccctg acggaaaggc ctggcaacct gagaccatag catttatgca
     8041 acttgcagaa caaaataact tcgtgatggc cggaaacact cacggaggcg aagaagtgat
     8101 aaactaccca tgggatacat ggtcgaacct tacagccgat aacaactggt ggatttttgt
     8161 atgtcgtgaa tatgtggata ccgttcatat atattctcct gctaattaca tgacgtatct
     8221 taataatgga atcaccagag gatactcgtg gtatagcata agtggaggac ggcaggatta
     8281 catgaattat tttcataact gccgcgaggt aacaatggag ctttctaaag ttaaaaggct
     8341 ggcaacatca agtctgaaca catattggaa ttataactat cgttcgatga ttaattacat
     8401 aaaacaggta acttacgggg ttgcaggaac ggtaaccgat ctgctatcaa acgaacccgt
     8461 cagagcaaag atcacaatca acgggcacga taccgacaat tcacatgtct tttcggatag
     8521 ccagaatggt ttttatcaac gcctactcga ttcgggcact tataatatca ccttcagttc
     8581 gccaggctat cttcccctca ctgtcgaagg cgtaaatgtt caaaagtatc aaaagactgt
     8641 actcaacgtt aatttggatc ccggccctct caatcctgtt atatcaagct ctgccagggc
     8701 tatcccgatt gaatcaacgg ttacttttac cgatgaatca ggaggtaagc ctgtatcgtg
     8761 ggaatgggct tttcagggtg gaacgccaaa tagttcctcg ttaaaaaatc ctgagccagt
     8821 tttatactct accgcaggaa ccttcgatgt caaattgagt attacaaaca gtaatggcga
     8881 aaagatcact cgtgtttttg aagattatat aagagtggcc ccaatagaac tcatgagtaa
     8941 taaatcagtt accacatgca actccttatt ttacgactct ggaggcgaaa acggaagtta
     9001 tgggaataac caaatgttaa ccttgacatt cctcccaggt ttgtcaggtg caatggtaga
     9061 ggccgatttt ttagaattcg gtcttgaggc tagtagtggt tgtatttacg attatctgcg
     9121 aatattcaat gggagtaata ctgaagctcc attaattggc gtatggtgtg gagccaattc
     9181 gccaggaaaa ataagcgcaa ccaatcccgc cggctcgcta acttttcagt ttaaatccga
     9241 tgcttacgtt acatcaacag gatggaaagc tctaatatcc tgtaaaacaa cggaagaaat
     9301 aataatagag aatggttggt ccgggatatc ctcatacgtt gtcccaatag atacaaatat
     9361 tgacactctt tttcaatcta ttattcaaga cattgttgtt gtaataggtg atgatggaat
     9421 ctattcgcct gagttgaata tcttcacttt gcaagactgg aactcacggc aagcttattt
     9481 agtgaagtca acttccccac agcttcttcc aatgaccggc acattgatgt taaacagaaa
     9541 tatttccctt aaccaaggtt ggaacctggt acacatacct acaccacaac aaataaaaac
     9601 ttccagtatt cagtcaattt taaatgacga tctaataatg ataaaaccgg ttgcagatat
     9661 tcatctcttc tggccgcaaa tgggaatcaa cagccttacc aacctcttgc cggggaaagc
     9721 ctactatttt aatgtttcaa aaaaagtaga gttcatgttc cctgataaat agtctttgta
     9781 taaccttatt ttaaataata aaaatagaga aagaacgatt aataattaaa aaaatcacta
     9841 tgagaatact attaacaagc atcctattat gttttgctat gatgctatca gcacaatccg
     9901 acattgatgt tgtaattatg aaaagcggtg tggagcataa atgtaccatt caggaaatta
     9961 agaatgacac tattgttatt tcccaatttt tagggcgatc gaaagttttt gaatcaatac
    10021 ctattaatga ggttgctact tacatactca ataatcatta ttcatcaccc ggtgaagatc
    10081 tgatgaaggc taccggactc ttctttgtag ctaccggatt tatggtaact ggcagtgttg
    10141 tggccgttat ggcgctcaat gcagacaata aagacctcgc attagcagga acagcgttcg
    10201 gactttttgg atcggtattc ctatttagcg gctattcaaa gatgcaatca gcaagtaaga
    10261 aactaaacaa gctgcatttt gaaaatgaca ggattattta caaattatag gaatcacata
    10321 ccattccttc tttcaaaata ttatctgagt gcaaacatta agaagccaaa ttgctaactt
    10381 ctgtatttgg gagtaaaggt ttatttctta tttgttaaag atttcgatga tgaatactcc
    10441 atggcggatt tcacaaaact aacaaacagg ggatgtggat tggcaacagt gcttttgtat
    10501 tcgggatgaa actgcacacc aataaaccat ggatgatttt ccaactcgat tatctcgaca
    10561 agttcagtct gtaaattaat tccagattgt ttcatgccgg cggtttcaaa ttgttctgag
    10621 aatttattgt taaactcgta cctatggcga tgtcgctctg aaatattttg agtaccgtaa
    10681 atctgaaatg cttttgagtt gggtttcaga aaacagtcaa atttgcctaa cctcatagtg
    10741 ccgtccagtc cagcgatttt cttctgatcc tccatcatgt caataaccgg ccacggtgtt
    10801 ttctgattaa actccagcga attagccttt tttaaaccaa gtacatttct tgcaaattca
    10861 attacagcac attgcatacc cagacaaata ccaaaaaaag gaatcctttg taaacgagca
    10921 tagttaacag ctataatttt accctcgaca ccacgtggtc cgaatccagg agcaacgaga
    10981 attccatcaa acccctccat taagttaccg tttccgttct cttcaatatg ttcagaatga
    11041 actgttttta cccgaacctt gcaattattt attgctccag cgtgtataaa tgattcaaaa
    11101 attgacttat aggcatcctt cagttctaca tatttaccta ttaatccaat cgtaacttca
    11161 gaagtaggat ttttaatcct atggagaaac tcctcccagc cttctaagtt tggttcatgc
    11221 ttcgaagtca ttttcagttt tcgcagtact tccagatcaa ggcgctcctt ttgcatcatc
    11281 aaaggaacgt cgtagatagt atttgcatca agcgactcta tcacactcga atcatcaacg
    11341 ttgcagaaca aggcgatttt tgcttttaaa gaattggtta gaggtcgttc tgtacggcat
    11401 acaatgatgt caggttgtac accctgttcc aacatggttt ttacagagtg ctgagttggt
    11461 tttgttttca actcttcagc agcagcaaga tatggaacga gcgtaagatg aatgaccagg
    11521 caattagagc caagctcacg acgcatctga cgaactgcct caatgtatgg caatgactca
    11581 atatcgccaa ccgtcccgcc gatttctgtt attacaatat cgtatttacc actatttcct
    11641 aaaagcttga ttcgatattt aatctcatcg gtaatatgcg gaataacctg gaccgttgag
    11701 ccaaaaaatt caccttttct ttctttgtta ataacagcct gataaatctt acctgtagtt
    11761 acgttgtttg cttgcgaggt aggaacgttc gaaaacctct cgtaatgacc aaggtcaagg
    11821 tcggtaacag ctccgtcttc tgttacataa cattcgccat gctcatacgg gtcaagtgtg
    11881 cccggatcaa catttatgta aggatcaagt ttctggatag ttattgaata cccacgagct
    11941 tgtaaaagtt ttgccaacga aacggaaata atcccctttc caaggctcga tgttacaccg
    12001 ccggttacaa atatatactt ggtgtctgcc attgaatttt gtttaaagaa tttacaaagt
    12061 aaataatttt agtcctgatg tcatcagact tacataaata aaaaattaac aaaggcaaac
    12121 ttacttagtt tctattcggc ggaagtttta ttctaacggt taaaaatgtt aaatagaggt
    12181 taaacggcac tttttagtat tatcaagtgt gattggttcc atacaagtat cttgctaaca
    12241 aattttaaat tactattgta ataaaactgt tttaaggatc ggacaaaata tttgcttttt
    12301 attctttttc agctttttgg cgaatatagg tatcaatttg tttggcagcc ttacgtcctg
    12361 ctcccattgc aagaattact gttgctgctc ctgttacggc atcgcctccg gcaaacacac
    12421 cttcgcgtga tgtctcccct gtttcttcat tagcaactat tcctccccaa gattcagttt
    12481 ttagccctgg agtagttttg gcaataagag ggttaggcga tgtgcctaac gacatgataa
    12541 caacatcgca ttcaatctca aactccgaac cttttatttc aatcggtctg cgccgaccgc
    12601 tatcatcagg ctcgccaagc tccattttaa tacaacgaat agccttgacc cagccattct
    12661 catctccaag aatttcagta ggattattca gtaatctaaa aatgatattc tcctctttag
    12721 catgatgaac ctcttcaaga cgagcgggga gttctttttc gcttcggcgg taaacaagat
    12781 aaacctctgc acctaaccga caggcagtac gtgcagcatc catggcaaca ttgcctccgc
    12841 ctaccactac aacgcgtttt ccaacatata ccggtgtgtc gtattcagga tcaaaagcct
    12901 tcatcagatt ggttcgggtc aaaaactcat tagccgaaac cactccatta agattttcac
    12961 ctggaatatt cataaatttt ggtagcccgg cgcccgaccc tataaagaca gcatgatacc
    13021 cttcagtatc taatagctca tcaatagtga tagtacgacc aacaataacg tcggtttcga
    13081 tattaacccc taagcgaact acattatcaa tttcatatct gaccactctc tcttttggca
    13141 aacgaaactc aggaatacca tattcaagca ctcctcccgg gcgatgcaac gcctcgaaaa
    13201 ttgttacttg atagcccatt ttagccagat cggtggcaca agagaggccg gcagggccgc
    13261 tacctataac tgcaacttta tagctattct gttcgacagt ggtcggttta taataatttg
    13321 ttgtcctacc ccaatcagca acaaagcgct ctaacttacc aatagatatc ggttctcctt
    13381 taatacccat aacacaagat ccttcacatt gtacctcctg tgggcaaaca cggccacaaa
    13441 cggccggaag acttgaatct tcagcgatta tatcaacagc gtcaccaaaa cgactttctg
    13501 ccactgcttt tatgaactgc ggtattttga tataaacggg gcacgcctga acacagcgtg
    13561 gatttttaca ttgaatacag cgctcagcct cttgtattgc ctcgctggca ttatatccaa
    13621 aacatacttc atcgaaattg gtgatacgct cttcaggaag ctgactcctg acgggaacac
    13681 gatttttttt aattgccatt atctttaaag accaatttta catttatgat tttcaagctc
    13741 taattctgct attttttgcc cctctatggt acgatacata ttttgacggc gcattgcttc
    13801 atcaaaatca accagatgag cgtcaaactc agggccgtca acacatgtaa aacgtgtctt
    13861 tccgccaata cttaccctgc aagcgccaca catgccggtg ccgtcaacca ttaaagtatt
    13921 aagacttgct attgttttaa taccatactc tttagtaagc atagtaacaa atttcatcat
    13981 gatcataggc ccgatcgtaa ccacaagatc atatttttta ccttgattct caataaggtc
    14041 ttttagcctg tcggtaacca atcctttgaa actagcgctc ccatcatcgg tggttaggta
    14101 gtatccatta acaacagcac gcatctcgtc ttccaatatt acataatctt tgttttttgc
    14161 gccaattata acatcagctt taaagcctcg ttcgctcaaa tatttaacct gaggataaac
    14221 aggcgctgtt cctactcctc caccaatgaa aagaatccga gtgttagcca actcctcgtc
    14281 agtcatatga ataagctctg aaggctgccc taaaggacct gcaaaatcga gcaaagcctc
    14341 tccttcttga agagcgcaga taagttttgt tgatgctcca attgattgag taacaatagt
    14401 tacagcgcca tcttgacgat taaaatcagc aatagtgagc ggaatacgtt cacccttttc
    14461 tcgaacccgt acaataacaa actgaccggg ctgtgccgat tttgcaactc gtggcgcatt
    14521 tacctccatc aggtaaatat ttggagccaa taatcttttc ttaactatag taaacataaa
    14581 atagactttc aagtgtatta taaactgcaa aattaatctt ttgggaaatt ttacaaattg
    14641 ttattgcaaa aagatactaa cacaaagagc ttcaaagaca tctgtaactg tttttttttg
    14701 tcgaatttgt tgataatcaa ctaagtaaaa atccgctttt aatagataaa tcaattagat
    14761 attattttga ggatcaaata tttaaaaaaa tattcatagc taaataaaat aggtaaaatt
    14821 gattaggtgt tttgagctaa aaaacaaaca atatctttca atgcctcatt taagatgagc
    14881 ttttaatgtg cagaagtcaa tttaaaagaa aaatataaat ttgaagtcaa tatttcaaca
    14941 tctgaaaaat aacaatttat gaataacttt gagatcattt cacttattct gtacttgttt
    15001 ttatttgttt caatgatggt cattatcttt ttcggaattg actttttacg aaacaaaaaa
    15061 gcctctcaat ctgaaacaat tcgaataata accccactga ggttacaggc ttacgaaagg
    15121 ataatactct atcttgagag gatttcaccg caggttatag ccaccagatt attaaatccg
    15181 gttttgacgg cgcgtgagtt tcaaaacact atcattcaaa gtatcaatga agagttcaac
    15241 cataattaca cacagcaact ttatgtttcg gacgaaacat ggaaaaaggt tctgttagtc
    15301 aaagaaaacg ttattagatc ggtgaagata gcctttgcca cgcttcccga aaaagcaacg
    15361 gccagcgatt tggcttcaag gttgttagaa gataaagagc aacaagtaca aaaagaaatt
    15421 agccaggcaa tagcctatct aaagaaagag gctcaatctt tattttgaaa agttagaccc
    15481 ggatattttt tttccatccc ttcatatttc ggaagtacaa gaccatccac cgttcttact
    15541 gcaaggttac tcttaccatg agcaacaatt ttattcaaat cgttgatagc cttaatttcg
    15601 acatttagca gcgatttacc tttgcgtagc acagaagcct caattgtgat ggcatcattt
    15661 tcatgaacac tttcaagaaa atcaacataa aagttgatag ttacgtataa cttatcttcc
    15721 ccgaggctgt aaaccgccac accaagagca tcatccatca ttgtggtaaa aattcctcct
    15781 tgtagcgtgc caagcgggtt tgtcatctct ttccttacaa tgtatctgat acaggctttt
    15841 ccctcttcaa tagagactaa tgttccattg agccagaccc cgatcggtga tggagagctt
    15901 tttaaatctt taccaacaag ggtcttcaga aattcaagta catgattggt cattatgctt
    15961 tcctatttat taatgatcat tttccatttt catcattttt cgagtattga aatatttctc
    16021 gcaggatatg atcatatttt ttgtaaataa tatttcttct aagctttaat gttggggata
    16081 gctcgccgct ttgcggtgac cactcttcac acacaagtct aaatcttttt attttttcat
    16141 gatctcccag ctctttattt accaaatcaa cttcacgttg aattcgttct attaccaacg
    16201 tttttttaat aagttcttca ttatcgcggt agtgtatctt gtatttggaa gcccaaaaat
    16261 gcaaatagtt aaagtttggt gaaataagtg cgctggcaaa tttttcattc tcccctacta
    16321 ccattatttg ttcaataaaa attgactctt taaacttatt ttcaatcagt tgtggtgcaa
    16381 catacttacc tgaggagagc ttgaatatct ccttttttct atctgttatt tttaaaaatt
    16441 ctccatctac catcatgcca atatctcctg tatggaacca cccctcctca tcaaatactt
    16501 gttttgtata ttcagggtct ttataataac ccatcataac attagggcct tttacaagta
    16561 tttcgccctc ctcatcaaac ttcacttcaa ccccttccag gattgggccg accgtgccaa
    16621 actttatttt aggccaaaaa ggatacccca cagctataac gggtgaggtc tcggtaagcc
    16681 catatccctc aacgactttt atgtctgctg cccaaaatat cctcgacagg aggggttgta
    16741 atgcagcgcc tcctgatacc acaacctgta ttctcccgcc aattgcagct ctccattttg
    16801 aaaatacaag cttgtcggca attttaagtt ttagctcata aaatttacca ttttgaccat
    16861 ttagctcgta tcgagaacca agttttaaag cccattgaaa tatctccttt ttcaaccatg
    16921 gcaaatcctt tccttgagcc acaatttttc cataaatttt ttcaagtact cgaggaacag
    16981 ccgtaaagcc atcagctttt atctcggtta agtcggctgc tatagtaccc atattttcag
    17041 cataatagat actgataccc agatattggt aatgataatt catcattcgt tcgaaaatat
    17101 gacataatgg taaaaaactc aataccttat gaccatattt aagtggttgt attttggaag
    17161 ttgaaataaa attggtaacc agatttctgt gagaaagcat aacccctttt gctgtaccgg
    17221 ttgtgccaga tgtgtaaatg atagtcgcca aatcttcggg cttaattgtg tcttttatct
    17281 cattaaaccg ggttttaaaa tgagcttcat tttgttctcc taaagctata atctcgttcc
    17341 aattagcaac cccctccact tcattaaata tgtaaacttt ggttatcgca ggtattgtac
    17401 caattaactt ctcaagctta gcgtaaagtt gcttgtcaga aataatgact gcctttacat
    17461 cggaatgggt caaaatatat ttatactcat caaggctgat agtcggatat aaaggcacat
    17521 gtactattcc aacctgaagc attcccatgt caataaaatt ccactcagga cgattgtttg
    17581 taacagtagc tattttatct cccttttcga atccaagagc taaaagacca atactgaata
    17641 aatttgaata cctgatgtaa tcatcggtgc tgtattttat ccaattacca ttcttctttg
    17701 cagctaaaat ggcatcactc gggtaattgt tttttaagtg atccagaaga tcaaagattc
    17761 ttgttatttc cataaaaatt atttttcgtg cagcaaaagt aaaatctttt ctgtttttta
    17821 aattttattg gttccattgg gagctatgta aaaacctaca aataatataa taaaataggt
    17881 tcccaattaa ataattgtta aaaaaacgaa ttaaaaataa ctaatctaca attttttttg
    17941 aaatttgtta ttaataagct tttgaaaaaa tattgtctat tcgatttaat aacaagtgat
    18001 aattaaaata aattctggct ttactaccaa acttggaata cttaccatcc ttctgattaa
    18061 tattaccctt aaccaattac gtgctcagga aagatatgga atcacgctaa gcaatttcgg
    18121 cggaatcaat gccgccacta taaatccttc ttttactgta aactccaaaa cttttttaga
    18181 tgttaacttt cttaccgccg gactaacgat agaaaataat tttgtttttt atcataaaaa
    18241 tgatttcaaa ttctccgagc tgttacgtgc caatcccaat ctcccaacca atgaggtacg
    18301 tggcgaaggc cttgattatt ctttaaaata tcagaatctt accggccttg cgtatgttga
    18361 agctcttggc ccttcttttt ctttaaatat tggaaaacat gccggagggc tttttacgcg
    18421 aaccgtagca gcttcatcgt tcgttaatgt tccccaagac cttggagttc ttttatttga
    18481 aggaataaga tacaaacctt tgtatggaaa ggagatcagc ggacaaaaaa taaatgtaga
    18541 tgcccttggt tggtcagaaa ttggattaaa ctattcgtat aactaccttt cgggatatta
    18601 caacaactgg tcagtcggta tcaacatcaa gcagctcctt ggctactcgg gagcatactt
    18661 gtcggcagat gatttaaaac ttaatgtagt taacagggat accgttgata ttactaatat
    18721 tctcgccaat atcggatatt ccctcccgat aaattacggt tcgaatggtt actcaagaac
    18781 aaaaccatta ttccttggta aagggattgg attagatttt ggatttactt tccggagaaa
    18841 tctcgatttt gcgccaatgg gacgggtaac tcgatattgt gaatcatatt ttcaacctta
    18901 tcggtacaaa ttgggggtgg ccgtcagtgg attgggaaat atccatttta acaaaaacac
    18961 cagagaacat acatttgaga atagatcagc gctttgggaa aacattgata ctactgaatt
    19021 tagaaatata aacgatttct tcgaaaactt aagtagtgtt ttttatggag atcctcattc
    19081 gttgcctact gaggaagata atatgattgt tgggttacct actgttttaa atcttaatgc
    19141 cgattatcaa tatttcccca attggtatct ctcaggaatg ttagtactgc ctgttaaaag
    19201 tagtgattat caattgatgt ttccaaaaaa agctttgctc ggcataagat acgaaacaga
    19261 tgatttggag ttcaatttct caagttcatt ttttaatttt caaaaattgc atattggctt
    19321 tttcgtcagg tataaatatt ttagcattgg tacaagtaaa cttgaatccc ttgtgaaagc
    19381 tacaaatcta tacggcatta atctatactt ctctataaaa ttccatctaa taaaaggtta
    19441 ttgtctttcg ctaccaccaa aacacgactg tggcaggttt cagttttaaa gattcaaatc
    19501 ggtttttttt cattaataat ggttaaagga attcaaaaag tattattttt gccgcaagtt
    19561 tttatttaga gtgatctttt atcgtttgcg ataaactgcg gtttcacaat cggtaattta
    19621 atctctgttt tttataaatg gtgcatgtag ctcagtcggt tagagcattg gattgtggtt
    19681 ccaagggtcg tgggttcgag tcccatcttg cacccttttt ttttaacctt tcattttcga
    19741 tttcactctg tttttcatct tttttctcac gaaatcaaaa cagcaaacaa cttatttttg
    19801 ccccaaaata aggctgttcc gaatcgcaat ccccttaaat cgtaacaaga atttctaaag
    19861 cctttattat tgcttttcct gatcaggatt aataaaagat agtgtgtata tcaacgattc
    19921 aagctggcga gtaaggttct ttttgtcttt attaggataa tacacaaagc catcaacggt
    19981 aacaatccgt tggttttgct catcgagcac agtataattg ataaatggtc cacccatgaa
    20041 atcaccgtcc agttcccaca tcccacgcat ttctactgca aacattccat tgaattctat
    20101 tcgatggctt tccgggggtt caaccatcga aactctcata aaggaacctt cggaaggtcc
    20161 aagaacgtta tctttggtca ttcgatcccg taattttatt atttcgtcgg gaagaaaaac
    20221 agaggtgtcg tgataaggaa tggtatatat caagattcct tgactaaact cctgaccttc
    20281 gcgacgtatc catacaaaat tttgctgctc cttagctaca taaaagtttt tggggataag
    20341 tatgtttaaa tcaaacttct ttgtaataaa cttttttatt ttcaaatcct caattgaccc
    20401 gaatgcttgt gctgctctcc tgcgttcgtt gacattgaaa tacatcataa aggattcttt
    20461 tttctcttcg aactcttcga aaaatgtggc ctcgtcatga acggtcattt taataattcg
    20521 ttgaggttta gcccaaagat cagaatgggt ttcgataacc ggtttatcaa aactcgcatt
    20581 tatatctaca ataaatatat tgtgatgagt tttaaacatt tgtccaaagc ccggttcata
    20641 gataaacgct acgtcaaagg ttttctcagg ctggggaagg ccagtcaaga tctggccaaa
    20701 gaattcagat ataactgatc ctacatgacc tttatgtaat gtttcgttat tgactaccac
    20761 aagtaattca tttgttttcc ctgttgaagg cttgtgtccc gagggattgc tactacaagc
    20821 cgccaataaa aaggcaaacc aacagaaaag agcgcatggc cataatttaa aaagcacttt
    20881 catttaatct gatattgatt tgttatccag cagtggtaat tttaattttc tgccctacct
    20941 taagagcttt tgtgtttttt atgttattca acttttttaa tccttctacg gttactccat
    21001 tatactgctt tgcaatctcc cacaaagtat ctccgggttt tactatataa tagatataac
    21061 catcagattc agacgtactc tggttttctt gagatgcagt accagctact gcttcggcag
    21121 attttgatac tatgagcttt tgattaacat gaatagtatt ggatactaaa ttattccaat
    21181 tcttcaagtc atttaccgaa catttatact tggaagctat aagccccaga ttttctcctt
    21241 tttttactac atgaatagtg ttattagtat tgttactact tgctgtagct gcacttgatt
    21301 ttccgatatt cgtgggagga acgccgctaa catagagctt ctgtcctggg tgtatagtag
    21361 ttcctcttaa gttgtttaat gcctgtattg tagaaacggt cgttttatat tttctagaaa
    21421 ttccacctaa cgtttcgcct ctttttacaa tatgtatctg atacttaggc atctctttaa
    21481 tttttgccat taacttctcc ttttcaagtt ctgtttctgt ccgatattta taaatttctt
    21541 cctctttaga aacataatag gccgtaagtg tacgtggcag gcgcaatata tatttgttat
    21601 ccttggtagc cggaatcact ccttctttaa acgatgggtt aagatactta aggttttctt
    21661 tcgaaattcc aatcatttcc gaaattctgt cgaaggtaag aacatcatat acttgcacgg
    21721 tatcaattgc gttgtaaagg attccaggat gtgttggata aaggttgtgt tcggctgaat
    21781 gagtcattac gtaagccact gctataaagg caggaacgta accacgagtt tcgcgcggca
    21841 gaaaaggcca gatagcccaa taatttttta ttcctccaga gcgacgaata gctttattaa
    21901 catttccggc tcccgaattg taagctgcaa gcactaacca ccaatcttca taaatgtcat
    21961 aaaggtcctt aaggtgcttg caggcggcaa tagttgattt gtaaggatca tagcgatcgt
    22021 caacgtatga gtttaactcc aggccataga cttttcccgt attatacatg aattgccata
    22081 acccctttgc accggcaggt gaaccggcca acggatttaa tgctgactca accactgcaa
    22141 ggtacttaag ctccaatggc aaattgtatt gatctaaaag ctcttcaaac atagggaagt
    22201 aaatagcagc aagcccaaga atacgtgaag agagaccgcg tttttttatt ccatacacat
    22261 caatataacc cttaaccgtt tcattatata caaattccaa aggcgttttc ctgttcataa
    22321 tttcaatcct tgactgataa actgaatccg aaaacttggg gacaaaagct tcatcgtaat
    22381 tatagatgtt gagcttgttt ttatcgaaaa gggtttcatc gtctctgaaa aacttaagag
    22441 tagtcagatt atcgagcata taaagcgctc tcgactcatt taatagctcc tgtgtaatac
    22501 gaggatcctc cattttcgaa ttggagaata tgaatgttga atcagcaaaa tcaaacgact
    22561 caattaatgt attggtgtct gatgaaaaat caggagtctc aaacaaagca gaatcttcaa
    22621 agtcttcagc ctcaaaaaga tgtgacttac ggtcttgatc aaattttggc ttattctttc
    22681 cgtaactcgt ctgcgacatt tttttgtctt tatccgaatg tgataaagca aagtattctt
    22741 taaaggtaga gcaactatat gaaaacaaca atattaatac aaacacaata agttttagag
    22801 acctggaaat aagaaccatg tggacaatgt ttttataaaa aacagttaat tgtagatttt
    22861 ttaacaaagg caaatataca ctctttttta tgtacccatt aaacatgtta tcattttgca
    22921 gaaaaacgat gctgctaatg attttagact ttaatccaaa ttatttcttt ataattagta
    22981 ttaaaaggag ttttaataaa caaacactaa atcaagatta ccctctcata aaatcaataa
    23041 ttcaactccg aagagatttt tgttgattga tccgggtatt attaaatatt gcttcttttc
    23101 aaaaaaaaag cagttttact atgtattact tcatttcacc atttcgtacg ttgcgaaatg
    23161 agctttttat aaaaaaaggg agaaagacgc aattaattgg ttatcacagc cggggtttcc
    23221 ccgtggcaat atgcgtttcc tccctaattt aattttaaaa ttattgattg atattaatct
    23281 tcatcatcat cataataatc atcatcatcc aatggagctc cccaattaat attggaaagt
    23341 tttccctctt cgaaaagaaa atctatcagc gcatcatcaa aaataagcca ctgtcccttc
    23401 tcctcttcat tatcttcaag atcaatttct tcaatttcgt agtctgaata tccgttttcc
    23461 ttcataaggt taattacctc gtctatagtc agttcgaaaa ctttagcgcc gaaaaggata
    23521 gtttcaggat tttcagtttc gacattactc aaaaagagtc catcaacctc ctcataaaag
    23581 atcgaaatac catgatcatc aaagtattga atcatagatt catcaaagtc atcatcttct
    23641 tcgataaatt cttcttcggt aggctcgcct aagtaacgaa ttgcctcatc gggggtggat
    23701 ccaaattcaa gctctccaaa tccttcgagt ggcttgattt caagtgttaa acctttcata
    23761 aaattaaatt taaaaatgaa aatttattta aatggtgaat taatctcttt agacattttt
    23821 ttaaaaacta atttatcaac tgttgccgga ataaacttat taagcagaat ggataatttt
    23881 ccttctgttg taagtactaa tgatcttttt cggttaacta ttgcatctgc tattttttta
    23941 gcaacttttt cggcgctcat cattttctct tcattgcgag gtgtttcgtt ttgttgtgat
    24001 ccatcgcttg ttaacgcttt aaaacgtatg ttggatgctg taaatcccgg gcaagctatt
    24061 aaaacatgaa ggccctgttt caagttttca gtacgaatta ctccgagaag cccttgcata
    24121 gcagattttg aggcgctata acctgatctg gcaggtaaac ctacataccc tgcaatggat
    24181 gaaacgccaa ctaaagaacc tttagacgat aaaaggaaag gaagtgcata ttttgaacaa
    24241 taggctgtgc cccaaaagtt aacatccatc aatttgcgga taacatcaag atgtaagtca
    24301 ttgaaaaggg cgcgcattga tactccggcg ttattaatca ggatatctat cttcccaaat
    24361 ttgtcaattg tttgctcaat aaggttttta caatccttct ctatacctac atctgtgggg
    24421 actgccaata cttcactttt aaacaattcg tttacctcat gttgcattgc cgataactgg
    24481 tcggctgaac gggcagcggc aacaattttt gccccgcgcc tggcaaactc atacacaaga
    24541 gctttaccaa tacctgatga agcgcctgtt ataacaacta ctttgttatc aaaccgtttt
    24601 gtcacaggac tgataaaaat taaatagcgt ttttacgatg ctaaaaaaaa cacattttat
    24661 ttttggcaaa tttatcaata atgttttgat atggtataaa ttggataaaa tattttcggt
    24721 gaaattcatg gctatttaat atccatgagc ttgtcaactt gatttctggc gtcagaccta
    24781 attttttcgc tttgtctctg tgcttcacta acaagtttgt ccgaattatt ttttgcttca
    24841 gctatcagtt tttctgcctt tttatctgtc tcttttttaa gttgttcagc cgctttctta
    24901 gcagctgctt cagctattgg ccccttcttt tttgcgtcag caatcatatt ctccgactgt
    24961 ttctgtgctt ccgccttcaa tttgtctgct gcctcttgtg cagccctatt aatcgcatct
    25021 gcctgctttt gggcttcgtt aattatttgg gctgcttgtt tatcggcgtc tgccaatatt
    25081 ttatccgatt gtgcttttgc ttcagccttt cgtttttcta actctttgcg tgcctcatct
    25141 tctaacttct gcttttgttt gtcgagttcc tcctgagcgc gctttttcat atcgtcgagc
    25201 attgattttc ctgatccatt gccaggcata atttttacaa ccggatcgga tgttgttccg
    25261 gtaattttag cagtaatatt aaccttttct ccaagtttga aacttgttcc taatttattg
    25321 gcttcattca ccaaattttc taataccgaa ttggccgccc cgccaaattt tgtgcgtgga
    25381 atagtgatag ccatttcaaa atcaatagtt tgatcaaatg aaatccagcc gagtgtattg
    25441 gcatttatat cctgataact caattcaaaa ggttttaaat gcactttacc attaacaaat
    25501 tcgaaagaaa gattcactgg acccattaaa agccgtttaa gatcaggcat tttgagcaaa
    25561 tcagcaattt tattagctat gctgaaattc tcaataacga tatttgttgt tttcagatta
    25621 ccgcctccat tcatcgaaga gtaaacaggc atcatttgat tatcaagtaa tgttttgatg
    25681 tttaatgaag tattaaattt cccatctgtc ttttgtgcta tcggtgcaaa cttgttgatc
    25741 gttccgaaag ttgaatagga tttttgaata tcaatttggt ttaagttaag agcgagttca
    25801 gctttagcct gtgttgaatc tcgtgtatca tactttccgt ttaaaaacat tgtgccgcct
    25861 aacacctcca tatttaaacg cttgaggatc aattcctgat caacaataag catctcaccg
    25921 gtagtatttg ttaattcaat attatcaaag atcagctttt taaacagggc ttgcacggaa
    25981 aattcgatat tgtccggtat tttcacagga cttattgcca cagtgtcgtc aggtaaatca
    26041 ctattttctg ttttcggtag taaatcggtg atgttgaaat tattactatt aagttgcaaa
    26101 ttgcctttca acacaccatc ggataaaagg taagggataa aattctctaa ttttccatta
    26161 gctgacaggt cgttattatc aacctttaaa tcaagatgca agagattaac aaaacgtggc
    26221 gaaaaactta gctgagcctt ggcgatctct attgttttat caattgaagc tgagcgcatg
    26281 ataaatgaat ccaataataa agacccgtcg aaattaaaag cttcatattg tctcttttca
    26341 attgttgaca ttttaccatc aaggctgata ttcgcagtga tcttgccctg agggttttct
    26401 ccgttaggta gtggataaaa atctcctatt tccgaaaggt tgatcatacc atcaattttt
    26461 cctttcaatg ccaggtcgct taccggatta cgaacaaata actccattga taacttatta
    26521 cctcccaaaa acaggctcaa attaggaaca tccacctcaa ttttatcgaa atcaataccg
    26581 ccaggatatt tgatacttgc attaatattt atttcatcaa ccgattttgg aaggtcggga
    26641 taatgaaagc gagcattatt aattaaagca ttaaattcaa acccaggata actatcatca
    26701 ttataaatcc ctttaagatt accattgatt atggcatttc cggctgttga tatattcttc
    26761 agatcggttg catacattac cggaattagc gaaagaaaat ccttgaaatc agtgcgtttt
    26821 gacgaatagg taaaatccat tacataatct ccgttatccg gcatttcaaa cgtaccttca
    26881 aacaaaagaa aaaactgatt taacctaagt tcgctattgt taaaggtgaa gaggaatttg
    26941 ttaaaatcta tatccaatgg cgaatctaac tcagcatcta cctcttgcag tactcttatt
    27001 ccttcgtagg aggcagtgag tttttcggcg gtggtgtgag tttgaagagt agtattatcc
    27061 aaagtcaaat cgcccgacag ttcatggtta acattttcaa gtaacagata aaatgccagg
    27121 cttttgtcgt cataaacaaa ttttgaattg gtaatggtaa actttttgag ttttataagt
    27181 agcgacgaag actcttcttc tggtgttgct tcgatatcag tttcatcagt ctctttcatt
    27241 atttcccaat tggctcttcc atcgcttttt actaaaagat taataaacga attgttcagg
    27301 tttacttttt ttatctggat gtcatcacct cttaaaacac tcataatatc aatagttgcg
    27361 tgtagcgtag ggatataagc caatgtatcg ccttcaaaag gttcattatt gattaccgat
    27421 aattctgtaa atctgacgct gatatcagga aagttacgaa atagtgatag acctaccctg
    27481 ttgaaatcca cattcgcatt aatattttta ttgatttcat ttttaacaac agctacaatc
    27541 ttgtctttaa aaatgtacgg aacaataatc attaacaaca gaattagcgc aacgattatt
    27601 cctgatattt tgaaaaattt tttcatggta agtggattat atttttggat gtattttatt
    27661 aacgaaatga tgtacatgta tgtttcgtaa aaaatgatcg gctattacca atgcagccat
    27721 cgcctcaacc actggaacaa cacgaggtat cacgcaaaca tcatggcggc cggatatgga
    27781 tatttctttt ttattgccgt gataatcaat agttagctgt gcctttttta ttgacgggac
    27841 aggcttaaac gcaacacgaa accaaattgt a
//
LOCUS       DAOOAI010000015        26053 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_2469_length_26053_cov_5.630972, whole genome shotgun sequence.
ACCESSION   DAOOAI010000015 DAOOAI010000000
VERSION     DAOOAI010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 26053)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 26053)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26053
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2469_length_26053_cov_5.630972"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1846)
                     /locus_tag="PK345_02175"
     CDS             complement(<1..1846)
                     /locus_tag="PK345_02175"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018028318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HPX33858.1"
                     /translation="MKETISYFKGDELAAKVWMTKYALKNSNGDIFELTPDDMHHRIA
                     AEIARIDLKYPNPLNEPVFYEVLKDFEYIIPQGSPMAGIGNNFQVSSLSNCFVIGDDT
                     PSDSYGGIMKIDQEQVQLMKRRGGVGHDLSHIRPAGSPVKNSALTSTGIVPFMQRYSN
                     STREVAQDGRRGALMLTLSIKHPDAEAFIDAKMESGKVTGANVSVKIDNEFMRCVKED
                     LPYTQQFPVNSDNPIITKTINARQLWNKIIHNAWKSAEPGVLFWDTILTESVPDCYAD
                     QGFTTVSTNPCGEIPLCPYDSCRLLAINLYSYVENPFQENATFDFSLFIKHVHIAQRM
                     MDNIIDLEAEKVDRILLKIKSDPECDDTKMIEKNMWEKIKAKTLLGRRTGIGITAEGD
                     MLAALGLRYGSEEATEFAVKIHKTLATELYRASVNLAKERGGFPIFDYEKEKDNPMIQ
                     RIGNADPTIIEDMKKYGRRNIALLTIAPTGSVSICTQTTSGIEPVFRPTYSRRRKVNP
                     NDKNVTVTFVDEVGDSWEEFNILHPKFKVWLEVNGYKPDLVEKMSGDEIESLVSKSPY
                     HKATSQDIDWVSKVKMQGAVQKWVDHSISVTVNLPADTTENLVSEVYMT"
     gene            2515..3564
                     /locus_tag="PK345_02180"
     CDS             2515..3564
                     /locus_tag="PK345_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HPX33859.1"
                     /translation="MVKVNKIQIIKALEEVIFFPKGNNIVALKMVNDIVIDKNQITIE
                     IVFEDPKAKTAEIVENSSINAIKKHFPDLTQTIKIIRKAKALSYTDGINTIIAVASGK
                     GGVGKSTIAVNLAITLSQLGHRVGILDADIYGPSIPMMFDMMDERPMAEKRNGSEVII
                     PIIKYGLKVMSIGFFVDPSQALLWRGPIASNALNQLFNTTDWGELDYLILDMPPGTGD
                     IHLTMVQDIPVDGVAIVSTPQEIALADARKAFSMYNTDKIQVPILGLIENMSWFTPAE
                     LPQNKYYIFGKEGGKQLANELGLVLLGQIPLVQSICESADQGKPITYTNEPDILTNSF
                     VSLAENIIKRLKLIK"
     gene            3577..3828
                     /locus_tag="PK345_02185"
     CDS             3577..3828
                     /locus_tag="PK345_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013821630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HPX33860.1"
                     /translation="MKNNEELIRKVKNVIDQIRPYLQSDGGDIEFVELTSDNIVNVKL
                     LGACGSCPYSTMTLKNGVENAMKRAIPEIKSVEAINLDF"
     gene            4019..5443
                     /locus_tag="PK345_02190"
     CDS             4019..5443
                     /locus_tag="PK345_02190"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPX33861.1"
                     /translation="MKKFLLIVLIGLSVTLFGQTTDRPWLIGISSNYADFNAVEMSVR
                     RQLRDANWMGKTLPSQLKVARSLSKSFVFGAEFSFVTLESEKLNLIPTIKSNVSTDKF
                     WRMGGQFEYKFANGYLLKEDAIFDPYIFLGLNGSNISEKTYLAQSTGAGTNIWFNKWL
                     GLNFEGSYDYLFDFNDYFHYSFGVTFRLGKAKDSDGDGVPDHLDECPDVPGLKILNGC
                     PDRDGDGIPDHLDECPDVPGLAEFKGCPDRDGDGIPDHLDECPDVPGLAQFKGCPDRD
                     GDGIPDHLDECPDVPGLAEFKGCPDRDGDGIPDHLDECPDVPGLARFKGCPDRDGDGI
                     PDHLDECPDEFGTITNKGCPEIIQEVEKIDFHVKNILFEISSSKIKQESFSDLDEIIA
                     IMKKFPDSRFTIHGHTDNTGSDALNMNLSKNRAKSVKDYFISKGINASRLESDGFGKN
                     KPIDVNDTPEGRANNRRVEIKVIK"
     gene            5844..8867
                     /locus_tag="PK345_02195"
     CDS             5844..8867
                     /locus_tag="PK345_02195"
                     /inference="COORDINATES: protein motif:HMM:NF024915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX33862.1"
                     /translation="MNGSIYFRIGIIHLLFFLYLFSHAQQTKTYDDPQASYRQAVDLV
                     EKKQYGAAQHLFGRLISTLPAGESVMRLEAGFYDGLCDYYLNHPQAKDKFEDFVRYYS
                     DHSKTNLVFVYLGFIEYAARKYRYAQEYFEKVDPYRLNPELMPEYLYKLGYCYLQRED
                     LFKAKDAFFPILNTASEYRDGANYYYAYIAYQEKDYQTALFHFEKIDLKSDFYEEVPF
                     YQLQIYFIKNDFEQILTKGPALVEYYTKDKKKNSELARIVAEAYYQNKDYSKAIDYFE
                     KYLAGTQKVNSRDENYQLGYAYYKTAMYQKAIGYFEKAIKNKDEMSQNAYYHLADCYL
                     QTGQTKFAQNAFYTAYQISGNEDLREDALFNYAKLTFELAYDPYNTSMAALNQYINDF
                     PNSERIDEAYQYLINLFLSSKDYQASINAIEKLKKKNRDMQSAYQRITYLFGVQLFNS
                     GHYQPAIENFRKALNNNINQEYTARSRFWIAESYFQTGEYTRAIDHYDSFESSSGSSK
                     LDIFPLASYNLGYAYFKLKYYENAVEKFKKFVSRPGILGEPLLADAYIRLGDCYFISK
                     NFSQAVSYYDQAISRSPRESDYALYQKAISEGARGRTDQKISQLQNLVNLYPQSSYVD
                     DATYEIAETYLLRNDNQNAFKWFDEVIKQYPNSSYFNRSLQKIGLIYYNQNNFDNALE
                     ILKQLVIKHPNSTEARDALLTIRNIYMDKNQVHKYYEFAESVPSAKISASEKDSITYI
                     AAENFYLTNDCISAIEAFDEYINKFENGAFVLNAYYYKAECEFNLGKTNEALQGYEFV
                     NRFSKSRFSENSALRAAEIYLSNENYHKALENFSNLELIADHSTNIITAIYGQMECHY
                     KLNEYPEAIESSRKLRSLDRISNEQLIKSYYIAGKSELEMENLTKAKQELESTVELSQ
                     GAFGAEAKYLLATIAFKQGELKKVEDVVVELAGQYPSYEYWKAKGFLLLSDVYVSLGN
                     IFQAKETLQSIIQYYPDKDLKEIAIEKLNDIEKKE"
     gene            8925..10619
                     /locus_tag="PK345_02200"
     CDS             8925..10619
                     /locus_tag="PK345_02200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33863.1"
                     /translation="MRLKKSAIGLSLFLFIMLWITDLTFGQGRNQEVTIIAPYQPSIS
                     DVTKIPFKPEFKDTLVKAPEMSYSINSQRIPTLFEVQPLKPAFIQIDPEKLLHRNYLK
                     AGFGNYKMPYAEFYSNSLYSNKYSLGFYVRHLSSQGGIKDYPTSAFSKNNISVYGKRY
                     LTDKVVSGNLLYDRDVVHYYGVKQNADNVTLTDNDLIQRFSLFGADVSLASNSRSKNN
                     YLIALNFWGLNDIFKTKESQIGLKGAYSSGNKIINVAKEENLGANLHFDLYANNDSLN
                     HLVTVKTALNPYFRLNFDYLDLTLGFETVLAADTNSKTYIYPQAKLFFKIIPDYLRLY
                     LSVTGGLQRNTLKSTSRENPWINTLFSFNFTNTKYNFKGGVTGKINQLIDFNFSASYA
                     DIDNMLFFVNDNISPFNPNIKENFGNKFISVYDDVKVTTINFEGNYDAPNRLKTTLVA
                     TYRDFKLNNLDRPWHKPAIETSIISRYFLKPEIALTGELFYVGKSYARLQDADQNITE
                     KKINGYVDLNFGAEYFFNDKISAFAQVSNVTGTRYYRWYQYPSQRINVMAGAVFAF"
     gene            complement(10717..12894)
                     /gene="rnr"
                     /locus_tag="PK345_02205"
     CDS             complement(10717..12894)
                     /gene="rnr"
                     /locus_tag="PK345_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005644987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008997 - ribonuclease R activity
                     [Evidence IEA];
                     GO_process: GO:0006401 - RNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HPX33864.1"
                     /translation="MSRKNKSPKKNKANKAKLGKKAIIAGVRSHFIENPFGSFNYKQV
                     AHIMGVNDRTSKNLIKDTLEELAIKGELEQLRRGKFKIKPEKLTSEKAFKVTHEGKVD
                     MKQTGKAYVTSPEMGEDIFIGANHTGHALHGDIVKVRLFPRRKGHKPEGEIVEIIKRA
                     KTFFVGTIAKSAKVAFMISDSQNMPIDILIPLNSLNKAKHGEKVVVKMTEWPEHANNP
                     FGEVIKILGKPGDNEVEMQAILVENDFPLAFSKEVEAEASKISDVIPAEEIKKRRDFR
                     QITTFTIDPFDAKDFDDAISYRRLENGNHEIGVHIADVSYYVKPNGKIDKEAFQRGTS
                     VYLVDRVIPMLPEKLSNHVCSLRPKEDKLCYSAVFELDDNAKVKAEWFGKTIINSDRR
                     FDYEEVQHIIETGKGDLAEVINEVWKLASQLKEERFKKGAISFHTQEVNFKLDEKGKP
                     IEVIISEQKESNFLIEDLMLLANRRVAERIGRKRGAVEPKTFVYRIHDQPSPEKLNQF
                     IQFVAKLGYKMRISTERNLAKSFNELFEKIKGKGEENLVETLAVRTMAKAEYSTKNIG
                     HYGLAFAYYTHFTSPIRRYPDLMVHRLLQRYLDRKPSVNADEYEGYCTHASDMERKAQ
                     EAERASIKYKQAEYMSDKIGQEFDGLISGVSKYGIFVEIKENKCEGMIRLQDLDDDFY
                     YLDEENYKVIGHRYQREFKLGDPIRIRVKNIDIRKKQIDYEIA"
     gene            13188..14123
                     /locus_tag="PK345_02210"
     CDS             13188..14123
                     /locus_tag="PK345_02210"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HPX33865.1"
                     /translation="MDYKDYYNILGVDKKATKEEIKKQYRKLAKKYHPDRNPNNKEAE
                     ENFKNIQEAYEVLGDDAKRSKYDQLGANWKQFQEGGAGFDFSQWAQQGGGRSYRVSFD
                     NIFEGGSGFSDFFNSFFGGGDSFIHQEFGTQSKRRKSANIKGRDYQANLTISLHEAYH
                     GTSTILNVNDKKIRVPIRPGVKDGQVLRVRGKGEVSYSGGEPGDLLIKINVTKSASTK
                     VKGNDLYSDVKVDLYTAILGGKITIQTISKPVSINIPPETQNGKELRLKGLGMPVFGR
                     KNEFGDLYLKIVVHLPENLNDQEKALFNQLKTIRS"
     gene            14234..16549
                     /locus_tag="PK345_02215"
     CDS             14234..16549
                     /locus_tag="PK345_02215"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPX33866.1"
                     /translation="MFQKLLIPAKLLLIMLVLAGNLLYSQSATIRGFIYEKESGEPVI
                     FTNAYLLGTSYGSTTDINGYFAITQIPPGSYTLIVTYLGYDTLTEDVSLKANDLIQKN
                     LYLEKSTVLLDAVNVSAERQAVRTETRTSVVKVTPLDIKQIPSVGGKADLAQYLQVLP
                     GVIFTGDQGGQLYIRGGSPIQNKVFLDGMVIYNPFHSIGLFSVFDTETIRLADIYTGG
                     FNAEHGGRISSVMDITTRDGNKKRHTGKLGLSTFGANALIEGPIKKQLEDGRGSSSFL
                     ISAKNSYLEQSSKLFYDYVNEDGLPFNFLDLYGKVTISAANGSKVNLFGFNFTDKVNY
                     KAISEYSWDALGGGVNFVVIPGRTPVLMEGIFAYSSYDMQLDEEEKNPRNSSINGFNA
                     GLDFTYFQGKNEIKYGIEMNGFTTNFAFFNDIGQSISQEQNTTELGGFFKYKIAINKF
                     LIEPGIRAQWYASLSNFSLEPRLAFKYNYSDRTRFKIAGGFYSQNLISARSDRDVVDL
                     FYGFLSGPDQTLIDFKGEETNHKLQKSKHLILGIEHDLFRGFTINLEGYYKVFPQLTN
                     LNRYKIYDASNVPPGTPHLEYSDYIVEKGEAMGADLSLKYSKNRLYFWTAYSYAFVNR
                     YDGMQWYYPHFDRRHNLNMLTAYTHGHLRQWEFSVRWNFGTGFPFTQSQGYYEKLLFP
                     GGINSDYMTENGILGIEYGELNLGRLPTYHRFDVNIKRTFFFTEKSNLQLDFSVTNVY
                     DRPNVFYVNRISGETVRQLPIMPSIGATFTF"
     gene            16712..17281
                     /locus_tag="PK345_02220"
     CDS             16712..17281
                     /locus_tag="PK345_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33867.1"
                     /translation="MHEIEPYYYWRHLYIASDDERSPFYKRQYSEFEFTNAIYNFLIH
                     PQWDEMGSATLYLKVLYADYNRGFSIIELLGEWNDCLYNDIMYLKRNIIEPMIKEGIN
                     KFILIGENILNFHASEDDYYQEWFDEVEEGWISGINFRKHVIEEFVHCNIDYYIAFGG
                     PFNCVDWRTIDPVQLFEEIDKLMVKRLSM"
     gene            17284..17553
                     /locus_tag="PK345_02225"
     CDS             17284..17553
                     /locus_tag="PK345_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HPX33868.1"
                     /translation="MKKSVILNIYGKVQNVGFRYQTKKKADLLQITGFVKNMHDGSVY
                     VEAEGEENDLILFIQWCYSGPFWAKVEKVKQQSAPIQNFESFIVK"
     gene            complement(18087..18758)
                     /locus_tag="PK345_02230"
     CDS             complement(18087..18758)
                     /locus_tag="PK345_02230"
                     /inference="COORDINATES: protein motif:HMM:NF039343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular biosynthesis protein"
                     /protein_id="HPX33869.1"
                     /translation="MHSHLIPGLDDGVDSLEKSMILIKEMKKLGYSKLITTPHIQGEF
                     YQNSSGTILSELERVKRELKKQNVDIELQAAAEYLIDEKFKEKIEKGDLLTFGKNHLL
                     VEMSFFNEYPHWRDYFFDLQIAGYKIVLAHPERYSYFFGNWSKYEELKDRGVLFQINI
                     SSLSGHYSNEIKRIAEKMIEQNMVELLGSDLHNLNYLEAIKHARFERSLKELIGSGKL
                     INHLL"
     gene            complement(18978..19466)
                     /locus_tag="PK345_02235"
     CDS             complement(18978..19466)
                     /locus_tag="PK345_02235"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX33870.1"
                     /translation="MDNLNVSFRYNVLPDDPKIIEEIVRSTGFFYEHEIIVANELAKE
                     RLIKGFDSGYFFVFAEYKGIVVSYACYGPTPCTKGTYDLYWIVTHNDFRGKGIGVRLL
                     NETIRLIREDNGRLLVAETSTKDQYLPSRKFYEKNNFKCEAIISDFYATDDGKAIFVY
                     RF"
     gene            complement(19469..20428)
                     /locus_tag="PK345_02240"
     CDS             complement(19469..20428)
                     /locus_tag="PK345_02240"
                     /inference="COORDINATES: protein motif:HMM:NF014686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPX33871.1"
                     /translation="MKIVITYNKPGKNALEDELDILDEVALVSACLKELGHEVIKLPM
                     TLNLEKAFLFLKNEKPDLIFNLVESLQNYGAIVFFPPALFDILQIPYTGNPTVPMFFT
                     AGKTITKQALFNAGLPVTASYKPSEVADLPNGKYILKPLWEEGSLGLDEASVFEITGL
                     DNINLNSFANDLYFIEPYIDGREFNVSLLAINSEPVVLAVAEILFVDYPSDKPKVLGY
                     RAKWNEESFEFKNTVRTYDLGKNGNQIHKLLSDISLKCWKTLGLKGYARVDFRMDNSG
                     NPFILEVNANPCITPGSGFYAACEYAGYSFTQVVDKIIKEVLK"
     gene            complement(20445..21416)
                     /locus_tag="PK345_02245"
     CDS             complement(20445..21416)
                     /locus_tag="PK345_02245"
                     /inference="COORDINATES: protein motif:HMM:NF019121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HPX33872.1"
                     /translation="MFIGLTYDLKSEYLKKGFLPDEVAEFDSEETIEGIESALHKLGH
                     KTERIGNILQLADALVNDKRWELVFNICEGYYGMGREAQVPALLDAYKIPYTFSDPLT
                     LSLTLHKGLTKRVIRDAGIPTAQFFLVENDKEAEICKLPFPVFIKPVAEGSGKGVSAK
                     SIVHSQESFKLEVTRLIKEYHQPVLVEKLLTGREFTVGILGTGKEARVVGVMEVIIRH
                     DAEDSTYSRFIKENYINRVEYQLIKNEIYAICAEISLKVWRVLGCRDAGRIDLKMDEN
                     RTLNFIEVNPLPGINHVHSDLPIMLYKQGWNYIQLIDEIIRSAIQRF"
     gene            complement(21418..22698)
                     /locus_tag="PK345_02250"
     CDS             complement(21418..22698)
                     /locus_tag="PK345_02250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KamA family radical SAM protein"
                     /protein_id="HPX33873.1"
                     /translation="MSPLNDLNSIETSAVECSNEDKPPSSQSTSNYNVIENKDIQFST
                     STPDEIIQESTFKFLRTWFPSASINNWNDWRWQLKNSFTKPEQLSFLLKDFNDVGLDH
                     SNKSMLPLRITPYYASLLHGAAPDDPLRKSIIPSAFELTLSKGESIDPLNEENDSPVA
                     NVIHRYPDRVLFLSTGFCSAYCRYCTRTHIVSKKDKCHTGVKAWEPGLAYIAAHPEIR
                     DVVISGGDPLTMSDSHIKYLLMRLRAINHIEIIRIGTKVPVVLPMRITKSLVSMLRRF
                     HPLYMSIHFTHPSELTKEASQACKRLADAGIPLGSQTVLLKGINDDVATMTKLMQGLL
                     KIRVKPYYIYQCDPIPGSAHFRTSINKGIEIIKGLRGHTSGYAVPQYVIDAPGGGGKI
                     PLLPEYYKGHDDQGRAVLENYQGMIYYYPDNQIE"
     gene            complement(23307..24809)
                     /locus_tag="PK345_02255"
     CDS             complement(23307..24809)
                     /locus_tag="PK345_02255"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPX33874.1"
                     /translation="MRSLFKHFINLTIFLLIANPLISQQVKKKMTLSDAIVIAQRQSP
                     DALSAKNRFLKSFWEMRTSEATLLPKLSLDATVPRYTRAIEKIVQPDGSEAFRERSLA
                     NTSADLSLSKNIALTGGQVYLQSGLQRIDYLNIGDAAGRPPTAWLSTPLVIGYSQNIF
                     GFNTYKWSKKIDPMRYNEAKRKYLEEIEQISINTTNLFFNLLVSQIQKNVAEQNIAYY
                     DTLYKIGLGRFNLGKIGENELLQLELGFLNAQANYETSALELEDRMFKLKSYLRLQDD
                     IPIEPTPPIQEVSFFEIPADKAVELANKNNSQALSFDRRLLEADRQVNIAKTTDRFSA
                     NMFAQYGLTQSANSLSEVYHNTQDQQLLVFGIKIPILDWGLAKGRIKVAESDREIVRT
                     SIEQERVDFDQSVFLQVMDFKMQQRRLQIAGKSDTVAWKSYEIARQRYLVDKVSITDL
                     TTSQINSDNSKINYIRSLQNYWINYFQIRKLTMFDFDKNEEISINFDLIR"
     gene            complement(24812..>26053)
                     /locus_tag="PK345_02260"
     CDS             complement(24812..>26053)
                     /locus_tag="PK345_02260"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX33875.1"
                     /translation="LGIIFGVAAVIAMMAIGNGARQEILEQIKMVGVNNIVITPKIED
                     KEKQQQSDDTEVASQIHKKSSPGLTLADATSIREIVPGVQKVSPEVVYNTFVVKSGVR
                     GNARFSGIEPDFFEVYNLELFEGKMFSNWQLENAAPVCIIGPDIRARYFKQENPIGKK
                     IKCGGIWLEVIGITKSRTVSETATKSMGVSEFNNSIFSPIKTVLLRYRDRSLITRATM
                     MSRNNDEQTSDNPNQLDKLIVQIHDSEKMTTTAKVIQRMLQRRHNGEEDFEIIIPELL
                     LRQQQRTKDIFNIVLGAIASISLIVGGIGIMNIMLASVMERISEIGLRKAIGARKNDI
                     IFQFVAEATIISITGGFIGILLGVILAAIITSTTGILTIVSMLSIIISFGVAATVGII
                     FGLMPARKAASQDPVTSLRHE"
BASE COUNT         8217 a         4745 c         4738 g         8353 t
ORIGIN      
        1 ctgtcatata cacttcactt actaaattct cggtggtgtc agcaggtaaa ttaactgtta
       61 cgctaatgga gtgatcaacc catttctgaa cagccccctg catttttact ttcgaaaccc
      121 aatcaatatc ttgtgaggtg gccttgtgat aaggtgattt actaacaagg ctttctattt
      181 catccccact catcttttcc acaagatcgg gtttataacc gtttacctca agccacactt
      241 taaatttagg gtggagtata ttaaactctt cccaggaatc tcctacttcg tcaacaaaag
      301 taacagttac attcttatcg ttcgggttaa ctttacgacg acgactatag gtgggcctga
      361 ataccggttc gatacctgaa gttgtttgag tgcagatgct aacacttccg gtaggggcaa
      421 tagtaagcag ggcaatatta cggcgaccgt attttttcat atcctcaatt atggtaggat
      481 cagcattacc aatacgctga atcattggat tatctttttc cttttcataa tcaaaaatgg
      541 ggaaacctcc acgttctttt gcaagattta cagacgcacg gtacaattct gttgcaagtg
      601 ttttatggat ttttacagca aattcagtgg cctcttccga tccgtaacgc aatccaagcg
      661 ctgccaacat gtctccttct gcagttattc cgataccggt gcgtcggcca agcaaggtct
      721 ttgcctttat tttctcccac atgtttttct cgatcatttt agtatcgtcg cactcagggt
      781 cagatttgat ttttaataat attctatcaa ccttttctgc ttccagatca ataatgttat
      841 ccatcattcg ttgagcgatg tgtacatgtt ttatgaaaag agaaaaatca aatgtggcat
      901 tctcctgaaa cgggttctcg acgtaactat ataggttaat tgccaacaaa cgacaactat
      961 cgtaagggca caaaggtatt tctccgcaag ggttggtaga aactgttgta aatccttgat
     1021 cagcatagca atctggaaca gattctgtta atatagtatc ccagaaaagt acacctggct
     1081 cagcagattt ccatgcatta tggattatct tgttccataa ttggcgggcg ttaatagttt
     1141 tggttatgat ggggttgtcg gaattaacag gaaattgttg agtgtatggt agatcttcct
     1201 taacacaacg cataaattcg ttgtcaattt tcactgaaac attagctccg gttactttgc
     1261 ccgattccat tttggcatct ataaaagctt cagcatcagg atgctttatg gatagggtga
     1321 gcataagtgc gccgcgtctt ccatcctgtg ctacttcgcg tgtggagtta gaatatcgtt
     1381 gcatgaaagg cacaattccg gttgatgtaa gtgcgctatt ttttaccggg ctacccgcag
     1441 ggcggatgtg agaaagatca tgaccaacac ctccacgacg cttcatcaat tgcacctgct
     1501 cctgatcaat tttcataatt cctccataag agtcagatgg tgtatcatct cctatgacaa
     1561 agcagttgga gagtgaagac acttggaaat tgttccctat tccagccata gggcttcctt
     1621 gtggaataat atattcaaaa tctttgagaa cttcataaaa aacgggttcg ttcaatgggt
     1681 ttgggtattt aagatcaatg cgggcaatct cggcggctat gcgatgatgc atgtcatcag
     1741 gagtaagttc aaagatatct ccattactat tttttagggc atatttggtc atccatactt
     1801 tggcagccag ctcgtcacct ttaaaataag atatggtttc tttcattacc tcttcgatgg
     1861 gaaatgcttt caatttagcc tttgaggcag tttttgcagg ctcaggtttt actattgggc
     1921 caaatagtgt ttgttcgatc gctttcggcc tttcacgttt tgtaattacc ttgtcataag
     1981 catttttatt tcctttttta acatgcgagc tttttacatc cgtcgtaggt tccgtttcca
     2041 aatctgcaaa aagatcaagt gtcataatat tttgattaat aataagttat taatttttag
     2101 tttttcatga aggctaatct tcaacaaatt aaattctaaa aaatttagag aaaaagctgt
     2161 gcaagataca accaaaacag acatcttacc aaatcaattt atccacaatt cgattgttaa
     2221 taaaattaac tacctgatat aaaatgttat atattgtaaa atggaaaaat ttatttttgg
     2281 ataaaaagtt tggcacgtgt tatttcaaaa atatcgaaac aagagattta ttaatccaca
     2341 ttcgacttaa gaaaaacagt aaaatttcat catttttgat ttcagttatt ggcaacgaaa
     2401 aacctctaat gtgtaacttc acacctcttg atttcatata atagcataaa tagatacatc
     2461 gaaaagtttg ttccatgcta ttttatacct ttgcggcact tttttttatt tattatggta
     2521 aaagtcaaca aaatacaaat tattaaagcc ttagaagagg ttattttttt tcctaaagga
     2581 aataatatcg ttgcactcaa aatggttaac gatatcgtta ttgacaaaaa tcaaattact
     2641 attgaaatag tttttgaaga tccaaaagct aaaacggctg aaatagttga aaatagcagc
     2701 attaatgcaa ttaaaaaaca ctttcctgac cttacacaga ctataaaaat tattcgtaaa
     2761 gctaaagctt taagttacac agatggcatt aacactatta tagctgtggc atcgggtaag
     2821 ggcggagtgg gtaaatcaac tatcgccgtt aatttggcaa ttacactttc ccaattgggc
     2881 catcgtgtgg gaattcttga tgcagatatt tatgggcctt caattccaat gatgtttgat
     2941 atgatggatg aaagaccgat ggcagaaaaa cgaaatggta gcgaggtaat tattcctatt
     3001 ataaaatacg gtcttaaagt catgtctatc ggattttttg tagatccgtc acaggcatta
     3061 ctctggcgcg ggccaatagc ttctaatgca ttgaatcaat tatttaacac caccgattgg
     3121 ggtgaacttg attatttgat tttggatatg cctcccggca ctggtgatat ccacctcaca
     3181 atggttcagg atattcctgt ggatggtgtt gctattgtca gtacgccaca agagatagct
     3241 cttgctgatg cccgcaaggc ttttagcatg tacaacaccg acaaaatcca agttccgata
     3301 ttaggtctta ttgaaaacat gtcgtggttt acacctgccg aactccccca aaataaatat
     3361 tatatttttg gaaaagaggg aggtaaacaa cttgctaatg aactcgggct ggtattatta
     3421 ggacaaatac cattggttca aagtatttgc gaatcagcag atcagggaaa accaataaca
     3481 tacacaaatg agccagatat cttaacaaac tcctttgtta gtctggccga aaatattatt
     3541 aaacgattga aattaatcaa ataaactttt ataaagatga aaaacaatga agaactcata
     3601 cgtaaagtaa aaaatgttat tgatcaaatt cgtccatact tacaaagtga tggtggagat
     3661 attgaatttg ttgagcttac ttctgataat atcgttaatg taaagctttt aggggcttgt
     3721 gggtcatgcc cttacagtac catgacacta aaaaacggtg tggaaaatgc aatgaagcgc
     3781 gctatcccag aaataaaatc agttgaagct ataaatctgg atttttaaca actaaattgt
     3841 taacattttt attcaaaagt ggctttttat cagtataaat attaactatc taaatgatta
     3901 agccatagtt gtaaaaattg tttaatttag ttctcaacga ataaaatatc ttttttaact
     3961 ttgcctagtt ttagcaaaaa ataatgaata atcaattaat taatttaaat ctacctttat
     4021 gaaaaaattt ctattaatag ttcttatcgg attgtctgtt actctttttg gccagacaac
     4081 agataggcca tggttgatcg gcattagctc caattatgcc gattttaatg cggttgagat
     4141 gtcggtaaga aggcaactta gagatgccaa ttggatgggt aaaacactcc cctctcaatt
     4201 aaaagttgcc agatcactta gcaaatcctt tgttttcggt gctgaatttt cgtttgttac
     4261 cttggaatcg gaaaagctaa atctaatacc taccattaaa tccaatgtta gtactgacaa
     4321 attttggcgt atgggaggtc aatttgaata caaatttgcc aacggatacc tgttgaaaga
     4381 agatgctatc tttgatccat atatcttttt aggactaaac ggatcgaata ttagcgaaaa
     4441 aacatacttg gctcaatcca ctggcgcagg aaccaacatc tggtttaata aatggctcgg
     4501 tcttaatttt gaaggatcat atgactattt atttgatttc aacgattatt ttcattactc
     4561 atttggggta actttcaggt taggcaaagc taaagatagt gatggtgatg gtgttcccga
     4621 tcatcttgat gaatgccctg atgtaccggg gttgaaaatt ctcaatggat gtcctgatcg
     4681 cgatggtgac ggtattcccg atcatcttga cgaatgtcct gatgtaccgg gacttgctga
     4741 gtttaaagga tgtcctgatc gcgatggtga cggtattccc gatcatcttg acgaatgtcc
     4801 tgatgtaccg ggacttgctc aatttaaagg atgccctgac cgcgatggtg acggtattcc
     4861 cgatcatctt gatgaatgcc ctgatgtgcc gggacttgct gagtttaaag gatgtcctga
     4921 tcgcgatggt gacggtattc ccgatcatct tgatgaatgt cctgatgtac cgggacttgc
     4981 tcgatttaaa ggatgccctg accgcgatgg tgatggtatc cccgatcatc ttgacgaatg
     5041 cccggatgaa tttggcacaa ttacaaataa aggatgccca gaaataattc aagaggttga
     5101 aaaaattgat ttccatgtaa agaatatcct cttcgaaatt agtagttcaa aaattaaaca
     5161 ggaatcattt agcgatcttg atgaaattat agctattatg aagaaattcc ctgattcaag
     5221 gtttaccatt catgggcata ctgataatac tggttcagat gcattaaata tgaacctttc
     5281 gaaaaatcgt gcgaaatcag ttaaagatta ttttatcagt aaaggaataa atgccagcag
     5341 gttagaatcc gatgggtttg ggaaaaacaa accgattgac gtaaatgata ctcctgaagg
     5401 cagagcaaat aatagacgtg ttgaaatcaa ggttattaaa taaccaataa aacacataat
     5461 taatttttgc cccggaaagg taaaatctat agccaatccg gggcattttt ttatagaata
     5521 agcaacccaa tggtattctt gatttaatgt ttgttcagta taattcttat cttattgatc
     5581 acatacaatt aatcacttaa ttaatgtaat tgcctaatct gatttaattt agcattaaaa
     5641 gatcgttatt tcgtttttgc taaaaaagta tgtagtactg attaattttg aagccgctat
     5701 taagaaatat actttcctaa aaacaggaaa taagtttgtt attccgtcaa atagcaggtg
     5761 ttatttctta acccgcctga tttcctaaaa aaatatctat caattgaaaa cgtttgataa
     5821 ttaatttttg aaaaataacc aagatgaatg gtagcatcta tttcaggatt ggtataatcc
     5881 atcttttgtt ttttttatat ttattttctc acgcccagca gacaaagact tacgatgatc
     5941 ctcaagccag ctatcgtcaa gcggttgatt tggtcgaaaa aaaacaatat ggcgcagctc
     6001 agcatctctt tggccgactg atttctacac taccagccgg cgagtcagta atgaggctcg
     6061 aagcaggatt ttatgatgga ttgtgcgatt attacttaaa tcatcctcag gcaaaggata
     6121 aatttgagga ttttgtgagg tattattccg atcattccaa gaccaacctt gtctttgtat
     6181 atcttggatt tattgagtat gcagcccgta aatataggta tgctcaggaa tattttgaaa
     6241 aagttgatcc atacagactc aatccggaat tgatgccgga atatctttac aaattaggat
     6301 attgttattt gcagcgtgaa gacctattca aagccaaaga tgcttttttt cctatcttaa
     6361 acacagcatc ggagtaccgc gatggagcta actactatta cgcctatatt gcctatcagg
     6421 aaaaagatta tcaaactgcg ctgtttcatt ttgaaaaaat tgatttaaaa tcggattttt
     6481 atgaagaagt accattttat caacttcaga tatattttat aaaaaatgat tttgaacaga
     6541 tattaaccaa agggccagcg ttagttgaat attacactaa agacaagaag aaaaacagtg
     6601 agcttgcgcg tatcgtagca gaggcttatt atcaaaacaa agattattca aaagctattg
     6661 attactttga aaaatatctg gcgggaacac aaaaagtcaa ttctcgtgac gaaaactatc
     6721 aacttggcta tgcttactat aaaacagcca tgtatcaaaa ggccatcggg tattttgaaa
     6781 aggccattaa aaataaggat gaaatgtcgc aaaatgcata ttaccatctg gccgattgtt
     6841 atcttcagac tggtcagacg aagtttgcac aaaatgcctt ttatacagct tatcagattt
     6901 caggaaatga ggatttgcgc gaagatgccc tttttaatta cgccaaactt acttttgagc
     6961 tggcatacga tccttacaac acatcaatgg cagcccttaa ccaatacatc aacgacttcc
     7021 caaattcaga aagaattgat gaagcttatc agtatttgat aaatttattt ttatcatcaa
     7081 aagattatca ggcttcaatt aatgctattg agaaattaaa aaagaaaaat agggatatgc
     7141 aatcggccta tcaacgaata acatatctgt ttggagttca actttttaat agtggacatt
     7201 atcaaccggc aatagaaaat ttcagaaaag ctttaaataa taacattaat caggaataca
     7261 cggcacgttc acgtttttgg attgcagaat cttactttca aacgggggaa tatacccgtg
     7321 ctattgacca ttacgattct tttgagtcat cgagcggctc gtcgaagtta gatatatttc
     7381 cgctggcttc gtacaatctt ggttatgctt atttcaagct taagtattac gaaaatgcgg
     7441 tagagaaatt taaaaagttc gtttcgcgtc ccggtatatt aggcgagccc ctcttagctg
     7501 atgcatatat tagattaggc gattgctatt ttattagtaa aaacttttcg caagctgtaa
     7561 gctactatga tcaggccata agtcggagcc caagagagag tgattatgca ctttaccaaa
     7621 aagctatttc agaaggagca cgtgggcgta ctgaccaaaa aataagccag ctgcaaaacc
     7681 tggtaaatct ttatccacag tcatcctatg ttgatgatgc tacctacgaa attgctgaaa
     7741 catatctgct acgcaatgat aatcaaaatg cttttaaatg gtttgatgag gtgataaaac
     7801 aatatccaaa cagtagctat tttaaccgtt cgctgcaaaa aatagggttg atctattaca
     7861 atcaaaataa ctttgataat gcgcttgaaa tactaaaaca actggtaata aagcacccta
     7921 attcaactga agcgcgagat gcgttgctca ctatcagaaa catttatatg gacaaaaatc
     7981 aagtgcataa atattatgaa tttgccgaaa gtgttccatc tgcaaaaata tctgctagcg
     8041 aaaaagattc gataacttat attgcagccg aaaattttta tttaacaaac gattgcattt
     8101 ctgccattga agcatttgat gaatatataa ataagttcga aaatggagct ttcgtattaa
     8161 atgcctatta ttacaaagct gaatgtgagt ttaatttagg aaaaacaaat gaagcgcttc
     8221 aaggatacga atttgttaat cgtttctcaa agtcacggtt tagtgaaaat tcagcattgc
     8281 gggcagcaga aatatacctt tcaaatgaaa attatcataa agctttagaa aatttcagta
     8341 atctcgaatt gattgctgat cattctacta acatcataac tgcaatatat ggccagatgg
     8401 agtgccatta caagttaaat gaatatcctg aggctattga atcatcacga aaattacggt
     8461 ctctcgatcg aataagcaat gagcagttga taaaatcata ctatatcgct ggtaaatcgg
     8521 agctggaaat ggaaaactta actaaagcta aacaagaatt ggaaagtact gttgaactgt
     8581 cgcaaggtgc tttcggcgct gaagcaaagt atttgttggc aacaatagct tttaagcagg
     8641 gcgaattgaa aaaggtagag gatgtagtag tagagcttgc tgggcagtat ccatcctatg
     8701 aatactggaa agccaaaggt tttttactcc tttcggatgt ttatgtaagt cttggaaata
     8761 tttttcaggc aaaagagaca ttacagagca ttattcaata ctatcctgac aaggacttga
     8821 aagagatcgc aatagaaaag ctcaatgata tcgagaaaaa ggaataattt attgttagaa
     8881 agatagcgtt aatgaaatta tcagaaaatc aatataagca cataatgaga ttaaaaaaat
     8941 cagccatcgg attgagtctt tttttgttta taatgttgtg gattactgat ttaacattcg
     9001 gacaaggtag aaatcaggaa gtaactatca ttgctcctta tcagccatcc atttctgatg
     9061 ttacaaaaat accattcaaa cccgaattca aagatacgct tgtgaaagct ccagaaatga
     9121 gctatagcat aaattcgcaa cgtatcccca ccctatttga agtacaacca ttaaaacctg
     9181 cttttataca aattgaccct gaaaaattac ttcaccgcaa ttatcttaaa gcaggatttg
     9241 gcaactacaa aatgccttat gctgagttct attccaacag cctttattca aataagtatt
     9301 cactcggatt ttatgttcgt catctatcct cacaaggtgg cataaaagac tacccaacaa
     9361 gcgcttttag taaaaacaat atatcagttt atggaaaaag atacctaacc gataaagtag
     9421 tttcagggaa tctattgtac gaccgtgatg ttgtgcatta ttacggcgtg aagcaaaatg
     9481 cagataatgt taccttaaca gataatgatt taatacaaag attttcactg tttggcgctg
     9541 atgtttcact tgcaagtaac agtcgcagta agaataatta tttaatagct ctaaattttt
     9601 ggggcttaaa tgacattttt aaaaccaaag aatcgcaaat aggacttaaa ggagcttata
     9661 gttcaggcaa taagataata aatgtagcca aagaagaaaa tttaggcgct aatcttcact
     9721 ttgatctcta tgcaaacaac gacagtttaa atcatttagt tactgttaaa acagccctta
     9781 atccttactt ccgtcttaat tttgattatc ttgacctcac gcttggtttt gaaaccgtat
     9841 tagccgccga cacaaacagt aagacatata tctatccgca ggcaaaactc tttttcaaaa
     9901 tcattcccga ctatttacga ctttacctgt cagttactgg tggtttacaa cggaacacat
     9961 taaaaagtac aagccgggaa aatccttgga tcaatactct tttttcattc aattttacta
    10021 atacaaaata taatttcaaa ggtggagtta ccggaaaaat caatcaattg attgatttca
    10081 actttagcgc atcatatgcc gatatagata atatgctgtt ttttgttaat gataacatct
    10141 ctccatttaa tcctaatata aaagaaaatt ttggcaacaa attcattagc gtttatgatg
    10201 atgttaaggt aactaccata aattttgagg gtaattatga tgcaccaaat agattgaaaa
    10261 caacattagt agctacttac cgcgacttca agctaaacaa tttagatcgg ccatggcaca
    10321 agccagccat cgaaaccagt attatttcga gatattttct aaaaccggaa attgccttaa
    10381 cgggagagct tttttatgtt ggaaaatctt atgcacgact tcaggatgct gatcaaaaca
    10441 tcaccgagaa aaagattaat gggtatgttg atctgaattt cggcgctgag tattttttca
    10501 atgataaaat atcggcattt gctcaagtaa gcaatgtaac aggcacacgc tattatcgct
    10561 ggtatcaata cccatcgcag cgtatcaatg ttatggctgg cgctgtcttt gcgttttaaa
    10621 gcaaaggaag gattattatt tgtttagtat tataagttga aaaaatctta ctgacaactt
    10681 cccgaaattt gttgaaagtt ctacatcact ctccgattaa gcaatttcat aatcaatctg
    10741 cttttttctt atatcaatat ttttaaccct tatcctaatt ggatcgccaa gtttaaattc
    10801 gcgctgataa cgatggccga taactttgta attctcttcg tcaaggtaat aaaaatcatc
    10861 gtcaagatct tgaaggcgta tcatcccttc gcatttattc tcttttatct caacaaatat
    10921 tccgtatttt gaaactcccg aaatcaatcc gtcaaattct tgcccaatct tatccgacat
    10981 gtattcagcc tgtttatatt ttattgaggc tcgttcagct tcctgtgctt tacgctccat
    11041 atcggaggca tgtgtacaat aaccttcgta ttcatcggca ttcaccgatg gttttctatc
    11101 aaggtaacgt tgcaaaaggc gatgtaccat caggtcggga taacgacgaa taggtgaagt
    11161 aaaatgggtg taatacgcaa aagccaatcc gtaatgaccg atatttttag ttgaatattc
    11221 ggctttagcc atggtgcgta cagcaagtgt ttccacaaga ttttcctctc ccttcccctt
    11281 tattttttca aataattcat taaaagactt agcaaggtta cgttccgtac taatacgcat
    11341 tttgtaacca agtttagcga caaattgaat aaactgatta agcttttcag gcgaaggctg
    11401 atcgtgaata cgatagacaa atgtttttgg ttcaacagcg cctcgcttgc gtccaatacg
    11461 ttcggccaca cgtcggtttg ccaataacat taaatcttcg ataagaaaat ttgactcttt
    11521 ttgttcgctg attattacct caataggctt tcctttttca tcaagtttaa agtttacctc
    11581 ctgagtatga aagctaatgg ctcctttttt aaaacgttct tctttgagtt gagaggcaag
    11641 tttccaaacc tcgtttatta cctctgccaa atcgcccttt ccggtttcga taatgtgctg
    11701 aacctcttcg tagtcgaacc ggcggtcgct attgattatt gttttaccaa accattcagc
    11761 ttttaccttt gcgttgtcat ccagctcaaa aacagcggag taacatagtt tatcttcttt
    11821 tggtctaagt gaacaaacgt ggttcgacag tttttcaggt aacataggga ttactctgtc
    11881 aactaaataa acggatgtgc ctctttgaaa cgcctcttta tcaatttttc cgttaggttt
    11941 cacgtaatat gaaacatccg ctatgtgtac tcctatttcg tgatttccat tttcgagtcg
    12001 gcggtagcta atggcatcat caaaatcctt tgcatcgaat ggatcaatgg taaatgtagt
    12061 tatttgccga aaatccctgc gttttttaat ctcttcggca ggtataacat ccgatatttt
    12121 ggatgcttct gcttccacct cttttgaaaa agccaatgga aaatcatttt caaccaggat
    12181 tgcttgcatt tccacttcat tatctcccgg cttaccaagt atttttatca cctcaccaaa
    12241 tggattattc gcatgttcag gccattcagt cattttcacc accacctttt cgccatgttt
    12301 agctttattt aagctattta atgggataag gatgtcaatc ggcatatttt ggctatcaga
    12361 aatcataaaa gctactttgg ctgattttgc tatcgtgcca acaaaaaagg tcttagccct
    12421 ttttattatt tcaacaatct cgccttcggg tttgtgtcct ttacgtcggg ggaacaaacg
    12481 cacttttact atatcgccat gtagggcatg tcctgtatga ttagcgccga taaatatatc
    12541 ttcacccatt tctggagagg taacataagc tttacccgtt tgcttcatat ccaccttgcc
    12601 ttcatgagta actttaaatg ccttttcaga agttagtttt tcaggtttta tcttgaattt
    12661 accccggcgc aattgctcca actctccttt tattgcaagt tcttcaagtg tatctttgat
    12721 aagattcttg gaggtgcgat cgttaacacc cataatgtga gccacttgtt tataattgaa
    12781 acttccaaac ggattttcaa taaaatgact tcgcacaccg gcaataattg cctttttgcc
    12841 caacttagcc ttgttagctt tatttttttt tggtgatttg ttttttcgag acatattttt
    12901 tacatttaag tatcgctaaa ttagtaaaac tatcaagaac gattttcaaa aatgttttca
    12961 atagcaattc gttttatcaa aacattattt tagataacgt atgaataaaa atgaaaatac
    13021 taatcttgaa aaaacttaca attaattcat cttttaattc ttgtaaattc atttttatga
    13081 aatcgtggca gaaacaacaa gttaaaagat ttaaaatcat agcattttga caaatattta
    13141 gcctttgcat caattttgat aaaaggacat ttcttaaata cgcaaaaatg gattacaaag
    13201 attattataa tattttaggg gtagataaga aggctacaaa ggaagaaatc aaaaaacaat
    13261 atcgcaaatt agctaagaaa tatcaccctg accgcaatcc taataataaa gaggcggaag
    13321 aaaattttaa aaatattcag gaggcctatg aagtattggg cgatgatgcc aaacgatcga
    13381 agtatgatca gttaggggca aactggaaac agtttcagga gggtggagcc ggttttgatt
    13441 tttctcaatg ggcacaacag ggaggaggtc ggagttacag agtgtcattt gacaatattt
    13501 ttgaaggtgg tagcggcttc tctgattttt ttaatagctt ttttggtggc ggagatagtt
    13561 ttatccatca agaatttgga acacaatcca aacgcagaaa gtcggcaaat atcaaagggc
    13621 gggattatca agctaacctc actatctcct tacatgaagc ctatcatggc acctctacaa
    13681 ttttaaatgt taatgacaaa aagataagag ttccgataag accaggggtt aaagatggac
    13741 aggtattacg cgtccggggc aagggagagg tctcatattc gggtggtgaa cccggcgact
    13801 tgctaataaa aattaatgta acaaaaagcg cttcaactaa agtaaagggc aacgatcttt
    13861 attccgatgt aaaggttgat ctttatacgg caattcttgg aggtaaaatt accattcaaa
    13921 ctattagcaa acccgttagt ataaatatac cgcccgaaac acaaaatggg aaagagttgc
    13981 gcttgaaagg acttgggatg ccggtatttg gaagaaaaaa tgaattcggc gacctctacc
    14041 tgaaaatcgt agtccattta cccgaaaatc ttaatgatca ggaaaaggct cttttcaatc
    14101 aattgaaaac tataagatcc taaccccttt ttatttgtaa ctcttattaa ttcatgtttt
    14161 gataataacc aaaggttagc gccgttagta agatttattg ttactaacta tagaaaaata
    14221 ttacttttat ctcatgtttc aaaaacttct gattcctgct aagttgcttt taatcatgtt
    14281 ggttttagca ggaaatctgc tctattccca atctgctaca ataagaggat ttatttatga
    14341 aaaggagagc ggtgagccgg tcatttttac caatgcttat cttcttggaa catcttatgg
    14401 ttcaacaacc gacatcaatg gctactttgc catcacgcaa ataccaccgg gcagttatac
    14461 tctcatagtt acctatttag gatacgacac acttacagag gatgtgtctt taaaggctaa
    14521 tgacttgata caaaaaaatt tatatctcga aaaatcaact gttcttttag atgccgtcaa
    14581 tgtttcggcc gagcgtcagg cggttcgtac cgaaacccgc acctcagttg tcaaagtaac
    14641 accattagat attaaacaaa taccaagtgt tggtggaaag gccgatttgg cacaatacct
    14701 acaggtactg ccaggtgtta ttttcaccgg cgaccaagga gggcaattat acatacgtgg
    14761 cggatcaccc attcaaaaca aagtttttct ggatggaatg gtaatttaca acccgtttca
    14821 ttctattggc ctcttttctg tttttgatac cgaaaccatc agattagctg acatttatac
    14881 aggagggttc aatgcggagc atggtgggag aatctcttcg gtgatggata taacaactcg
    14941 cgacggaaac aaaaaacgac ataccggaaa acttggatta tcaacatttg gagcaaatgc
    15001 tttaatagag ggaccgatca aaaagcaatt agaagatggt agaggaagtt catcgtttct
    15061 tatttcggct aaaaattcat accttgaaca gagttcaaag ctgttttatg attatgtgaa
    15121 tgaggatggc cttccattca attttttaga cctttatggg aaagttacta taagtgctgc
    15181 aaatggcagt aaagtgaatc tttttggatt taactttact gacaaagtaa attataaggc
    15241 gatatcagag tattcgtggg atgcgttggg tggtggtgtt aattttgttg tcataccggg
    15301 acgtacaccc gtattaatgg aagggatctt tgcttactcc tcatacgata tgcaattaga
    15361 tgaagaggaa aaaaatcctc gtaacagttc aatcaatgga tttaatgcag gattagattt
    15421 tacatatttt cagggtaaaa atgagataaa atatgggatt gaaatgaatg gttttacaac
    15481 caactttgcc ttttttaatg atatagggca aagcatttca caagagcaaa atactactga
    15541 gcttggcgga ttttttaaat acaaaatagc tatcaataaa tttcttatcg aacccggaat
    15601 cagagcacag tggtacgcct cactttccaa cttttcatta gagcccaggt tggcctttaa
    15661 gtataactat tctgaccgta cccgttttaa aattgccgga ggtttttatt cgcaaaacct
    15721 tattagcgca cgctccgata gagacgttgt tgatcttttc tacggctttc tctccggccc
    15781 cgaccaaact ctaattgatt tcaaaggcga agaaaccaat cataaactcc aaaaatcaaa
    15841 acatttaatt ctgggaattg aacatgatct ttttcgtggc tttaccataa accttgaggg
    15901 ctactataaa gtatttccac agctaacaaa cctgaatcgt tacaaaattt atgatgccag
    15961 caatgtacct ccaggaaccc cacaccttga gtatagtgac tatatcgtgg aaaagggaga
    16021 ggcaatgggg gctgaccttt ctttaaaata cagcaaaaat agattgtact tctggacagc
    16081 atactcctat gcttttgtga accgatatga tggaatgcaa tggtattatc cacattttga
    16141 tcgcagacat aatctaaata tgcttacggc atatacacac ggccacctgc gacaatggga
    16201 atttagtgta agatggaatt ttggtacagg attcccgttc actcaatcac aaggatatta
    16261 cgaaaaactc ttgttccccg ggggaattaa ctccgactat atgactgaaa atggaatctt
    16321 aggtattgaa tatggcgagt taaatcttgg acgcttacct acttaccacc ggtttgatgt
    16381 caatataaaa cgtacctttt tctttaccga aaaaagtaac ttgcagctcg attttagtgt
    16441 tactaacgtt tatgaccgtc caaacgtctt ctacgttaac agaataagcg gagagactgt
    16501 tcgccaattg ccaattatgc caagcattgg cgccactttc actttttaaa aaaaaagaaa
    16561 agcagcttgc ccttttgtta gaataaagct attggaaaat agaggctgaa taaaaaatta
    16621 taagatatta ttgtaaaagc ttttgaaatt aaaacagctt cgttaaataa ttgttagttt
    16681 tactaagaat aacaatcttt cacaaaaaga catgcatgag atagaaccat attactactg
    16741 gcgtcatctt tatatagcct ctgatgatga gcgttctcct ttttacaaaa ggcaatatag
    16801 tgaatttgag tttactaatg ctatttataa tttccttata catccacaat gggacgaaat
    16861 gggctcagca actctttatt taaaggtatt atacgccgac tacaacagag ggttttccat
    16921 aatcgagctg ctgggcgaat ggaatgactg tctatataac gacatcatgt atctcaagcg
    16981 caacataatt gaaccaatga ttaaagaagg gataaacaaa tttattctca ttggcgaaaa
    17041 catcctgaat tttcatgcat ccgaagatga ttactatcaa gaatggtttg acgaagtaga
    17101 ggagggctgg atttctggga taaacttcag gaaacatgtt attgaggagt ttgtacactg
    17161 caacattgat tattacatag cctttggagg tcctttcaat tgcgttgact ggagaacaat
    17221 agatccggtt cagctttttg aagaaattga taagttaatg gttaaacgat tatcaatgtg
    17281 atcatgaaaa agtctgttat cctaaatatt tatgggaaag ttcaaaatgt aggatttcgc
    17341 tatcaaacta aaaaaaaggc agacttactt caaataacag gatttgttaa aaatatgcac
    17401 gatggttctg tttatgtgga agccgaaggt gaagaaaatg atctaattct tttcattcaa
    17461 tggtgttata gcggaccttt ttgggcaaaa gtagaaaaag taaaacagca atccgcaccc
    17521 atacagaact ttgagtcttt tatagtaaaa tgaaatatta ctaaaaaaaa ccccccacct
    17581 ttaaaaaagt ggagggcttc tcctgataat aaattgaata tttctatttt accaaaccaa
    17641 tgatgaaatc attatttgac cactatttta taatgcttga gaagacatta tcggaaaaag
    17701 gatgggaggg aacttccgtt taataggtca acataaatat tcaattaata tcatcaaaga
    17761 acagaataaa aacagaataa gtaaacgcat taaaaattaa tgcggcgcta aaataatgca
    17821 aaaaaagtaa ccaaataaga aaaatgaatt cttttttaag tttactaagt tatttcgtat
    17881 aacttttgga tgatattaga aaactgatag acttaagatt taagtaaatg accggcgctg
    17941 gcagttattt ttcataatta ctaatttaca atcattttga ataacttttt cagagcggat
    18001 ccaatcttca ttttattttt aatacgaaat cctgtaaaac taaacgtcaa ttatatcaat
    18061 tgaacataaa actctgactt ttgtatctat aacaaatggt taattaattt tccagatcct
    18121 attagttctt taaggcttct ttcgaaacgg gcatgtttaa ttgcttcaag ataatttaaa
    18181 ttgtgcaaat cgctcccgag tagctccacc atgttttgtt cgatcatttt ttcagcaatt
    18241 ctttttattt catttgaata atgacccgag agcgaactta tattgatctg aaaaagcact
    18301 cctctatctt taagctcttc atatttgctc caatttccaa aaaagtatga atagcgctcc
    18361 gggtgagcaa ggactatttt gtatccggct atttgcaaat caaagaaata atctctccaa
    18421 tgtgggtatt cattaaaaaa tgacatttcg acaagtagat gattttttcc gaaagttaga
    18481 agatcgcctt tttcaatttt ctccttaaat ttttcatcaa tcagatattc ggcagccgct
    18541 tgaagctcta tatcaacgtt ttgtttttta agctcacgct ttactctttc caactctgac
    18601 aatattgtcc cagaagaatt ttgataaaac tcaccttgaa tatgtggcgt agtaataagc
    18661 tttgagtacc caagcttttt catctcttta ataagaatca tagatttttc aagcgaatcg
    18721 actccatcat ctaaaccggg aataagatga gaatgcatat cagttttcaa tagagataaa
    18781 tcaacgggtt tcgttaacct ggaggaagaa aatatgtttt ttaaaactga cattactata
    18841 aaattgaggg cgcaaaaata agattttgta aaaggtggtt catttcaaag aggtatctgt
    18901 tgattttacc agtaaacatt ttaaaatttc cgggacgttg ttttacaaaa atgaaacagt
    18961 gtaaaactat tgatggatta gaatctgtaa acaaaaatag cctttccgtc gtcagttgcg
    19021 taaaaatcag atataatagc ttcgcatttg aaattgtttt tctcataaaa ctttctcgaa
    19081 ggtagatatt gatctttagt tgacgtttca gcaacaagca ggcgcccatt atcttctcta
    19141 ataagcctta ttgtttcatt aagaagacgc acaccaattc cttttccacg aaagtcgtta
    19201 tgtgtaacga tccaatacaa atcataggtt cccttagtac atggtgtagg tccgtaacaa
    19261 gcatacgata caacaatgcc tttatattct gcaaatacga agaaataccc actatcaaat
    19321 ccttttataa ggcgttcttt agcaagttcg tttgcaacaa taatttcatg ctcgtaaaaa
    19381 aaacccgttg atctgacaat ttcttcaata atttttggat catccggcag aacattgtac
    19441 cgaaaagata catttaaatt atccataatt atttcaacac ctctttaata attttatcaa
    19501 cgacttgagt aaagctgtat ccagcatatt cgcatgcagc atagaaaccg ctcccagggg
    19561 ttatgcatgg attagcgtta acttcaagga taaaaggatt gcctgaatta tccattctaa
    19621 aatctaccct tgcatagcct tttaaaccca atgttttcca gcattttaaa gaaatatctg
    19681 ataaaagttt atgaatttga tttccgtttt tgcctaaatc ataggttcgt acggtatttt
    19741 taaattcgaa actttcttca ttccatttag ctctataacc cagcacttta ggcttgtcag
    19801 acggataatc cacaaaaaga atttctgcaa ctgctaagac tacaggttca gaatttatgg
    19861 cgagtaatga aacattgaat tctcttccat caatataagg ttcaatgaaa taaaggtcat
    19921 ttgcaaaact attcaagttg atattatcta atccggttat ttcaaaaaca gaggcttcgt
    19981 ctaatccaag tgatccttct tcccataaag gttttaaaat gtattttccg ttaggtaaat
    20041 cagcaacttc tgaaggctta tagcttgcag taactggtaa cccagcattg aaaagagctt
    20101 gtttagttat ggttttaccg gctgtaaaga acattggaac ggttggattt cctgtgtatg
    20161 gaatttgaag aatatcaaaa agtgctggag gaaaaaaaac tatggctcca taattttgta
    20221 aagactctac caaattaaat atcaaatcag gtttttcatt tttcaaaaac aaaaatgctt
    20281 tttcgaggtt cagcgtcatt ggaagcttta ttacttcatg tcctaactct tttaagcagg
    20341 ctgatactaa cgctacctcg tctaaaatat ccaattcatc ttccagagcg tttttgccag
    20401 gtttattgta agtaatgaca attttcattc gatactataa tattttaaaa tctctgaatt
    20461 gctgaacgta ttatttcatc aataagctga atgtaattcc agccctgttt atacagcata
    20521 atgggtaaat cagagtgaac atgattaatt ccgggtaatg gattcacctc aataaagtta
    20581 agtgttctat tttcatccat tttaaggtca atacgtccgg catcacgaca accaagaact
    20641 ctccatactt taaggctaat ctcggcacaa attgcatata tttcattttt aatcaattgg
    20701 tactctactc tattaatata attttccttt ataaaacgtg aataggtaga gtcttcagcg
    20761 tcatgacgga ttatcacttc cattactccc actactcttg cttctttacc cgttccaagt
    20821 atccctactg taaactctct accagtaagt aatttctcca ccaacacagg ttgatgatat
    20881 tcctttatca atcttgttac ttcgagttta aagctttcct gagagtgcac aattgacttt
    20941 gcacttacac ctttgccgga accttccgca acaggtttaa taaatactgg aaatggcagc
    21001 ttgcaaatct ctgcctcctt atcattttcg acgagaaaaa attgagctgt aggaattccc
    21061 gcatcccgaa tcactctttt tgttaatcct ttatgtaaag taagggaaag cgtaagcgga
    21121 tcggaaaaag tgtaaggtat tttataggca tcgagtagtg caggtacctg tgcctcacgc
    21181 cccatcccat aataaccttc acaaatatta aatacgagtt cccatctttt atcattaaca
    21241 agggcatcgg caagttgcaa tatattacct attctttcag ttttatgtcc taatttgtga
    21301 agagcagatt caataccttc aatggtctct tccgaatcaa attcagctac ttcatcaggc
    21361 aagaaacctt ttttgagata ttcacttttt aagtcataag taaggccaat aaacatatta
    21421 ttctatttga ttgtccggat aataatagat catgccttga taattttcca ataccgctct
    21481 accttgatcg tcgtgccctt tataatactc aggcagcaaa ggtatttttc ctcctccgcc
    21541 cggagcatca attacatact gtggaacggc ataaccagat gtatgaccgc gcaaaccttt
    21601 tataatttca atacctttat tgatcgaagt tctaaaatga gctgaaccag gaataggatc
    21661 acattgatag atataataag gctttactct tatttttagc aacccttgca ttaatttggt
    21721 cattgttgcc acatcgtcat taatgccttt caaaagaact gtttgacttc caagcggaat
    21781 tcctgcatct gcaagtcttt tgcatgcttg cgaggcctct ttagtaagtt cagaaggatg
    21841 agtaaaatgt atgctcatat aaagcggatg aaatcttctt aacatcgaaa caagtgattt
    21901 ggttatcctc attggtaaca cgacaggtac ctttgttcca atacgtatta tttcaatatg
    21961 attaattgct cgcaaccgca tgagcaagta tttaatatga gaatcggaca tagtgagcgg
    22021 atcgccacca gatataacaa catctcttat ttcgggatga gcagcaatat aggctaaacc
    22081 aggctcccat gccttcactc ctgtatgaca tttgtctttt ttagagacaa tatgagtccg
    22141 tgtacaataa cgacaatatg ccgagcaaaa tccagttgaa agaaaaagaa cgcggtcggg
    22201 ataacgatga ataacatttg caacagggct atcattttct tcatttagtg gatctataga
    22261 ttctccttta ctcaacgtca attcaaaagc agaagggatt attgatttcc ttaatggatc
    22321 atcaggagcg gctccatgta acaggctggc ataatatgga gttatcctta acggaagcat
    22381 acttttgttt gaatggtcta atccaacatc attaaaatct ttcaacaaaa atgaaagttg
    22441 ctccggttta gtaaaactgt tttttaattg ccaccgccaa tcattccagt tatttattga
    22501 agcggaagga aaccaggttc ttagaaattt aaaggtagat tcttgaataa tttcgtcagg
    22561 agtagatgta gagaattgaa tatccttgtt ttcaatgaca ttatagttcg aagtactttg
    22621 actactcgga ggtttatctt cattggaaca ttcaactgcg cttgtttcaa tagagtttaa
    22681 atcatttaaa gggctcatga tttatattta attaactgat ttttaatgtt tttaatttac
    22741 tgtcattttt caaaaaaaaa catacaatat taattcaaag tatgattagt ttttcatttt
    22801 tcaaaaaaaa cttttataga tgataaaaat atgagtgtca aaaggttgct gttaagttag
    22861 aatgagaata tcataatgct tatataaaga ggtatttgta attgctacaa gaagagtttt
    22921 attgatcaat gtgtaggttt taataatgta caacttataa atgttaatat gttgaactaa
    22981 ggtctgctaa ttcttttaaa agagtgggat ttggtttcat tgtcagaatc tgtattagat
    23041 atgcgtaaga ttgatttgaa cggtgttaaa aacagaagat ttgcattaga acctgttcta
    23101 tgccaaaggc tggcgtctaa caaatatttc acgtcctgaa gttaaaaggg gacttgtgag
    23161 atcggaaaca acatgtgtta tcttttataa aaaacagacc ttagtgaaac gctgtcattc
    23221 agcatgttcg atttattatt cctcagttta ggttaaaaga tacgggcttt aagcaaagct
    23281 attagttaaa taagtatttt tttgagttat cttattagat caaaattgat agaaatttcc
    23341 tcgtttttat caaagtcaaa catggttagc tttcgtattt ggaaatagtt gatccaatag
    23401 ttttgtaggc ttcttatata gttaatcttc gaattgtcgc tattaatttg cgaagtggtg
    23461 aggtcggtaa tgcttacttt atcaacaagg tatcgttgtc gtgcgatttc atagcttttc
    23521 catgctactg tatctgattt tccggcaatt tgtagtctgc gttgttgcat tttaaaatcc
    23581 atcacctgta aaaaaacact ttggtcaaaa tccacccttt cctgttctat agaagtgcgt
    23641 actatctctc tatctgattc ggctactttt atccttcctt tggccaagcc ccaatcgaga
    23701 attgggattt taattccaaa gacaagcagt tgttgatcct gagtgttgtg ataaacctcg
    23761 ctaagtgagt tagcgctttg tgtaagtccg tattgagcga acatgttagc gctaaagcga
    23821 tcggtagttt ttgctatgtt tacctggcga tcggcttcca acaggcggcg atcgaacgaa
    23881 agcgcttgtg agttattctt atttgccagt tcaactgctt tgtcggccgg gatctcgaaa
    23941 aaactaacct cttgaatcgg gggtgtaggc tcgataggta tatcatcttg aagcctcaga
    24001 taggacttta acttaaacat cctgtcttca agttctaagg cggatgtttc gtaattagcc
    24061 tgagcattaa gaaagcctaa ttcaagctgg agcagttcat tctctccaat tttgccgagg
    24121 ttaaacctgc ctaatccaat tttgtagagt gtatcgtagt atgcgatatt ctgttctgca
    24181 acattttttt gaatttgcga tacgagcaaa ttaaaaaaga ggttggtagt attgatcgaa
    24241 atctgttcta tctcttctag gtatttacgt ttagcttcat tatatcgcat tgggtcaatt
    24301 ttttttgacc atttgtaagt gttaaacccg aagatatttt gggaataacc tattactaag
    24361 ggtgttgaga gccatgctgt aggtggtcgt cctgcggcat cgccgatatt gagataatca
    24421 atcctttgca agcccgattg taaatagacc tgaccaccag tcagtgctat atttttgctt
    24481 aatgaaagat ctgctgatgt gttggctaag cttcgttctc tgaaggcctc gctgccgtcg
    24541 ggctgaacga tcttttctat ggctctggta tatcgaggaa cagtagcgtc aaggctgagt
    24601 tttgggagta atgtagcttc cgatgttcgc atttcccaaa agctttttaa aaaacgattt
    24661 ttggccgaca gtgcatcagg cgattggcgt tgagcaataa cgattgcatc agaaagggtc
    24721 atctttttct taacctgttg acttatcaac ggattggcga tcaataaaaa gatggttagg
    24781 ttgataaaat gtttaaaaag tgatctcata gctattcgtg tctgagtgag gtaaccgggt
    24841 cttggcttgc agcttttcga gccggcatca ggccgaaaat aattcctacc gtagccgcca
    24901 caccgaacga gattatgatt gatagcatgg aaacaatagt gagaatacca gtcgttgaag
    24961 tgataatggc agcaagtata actcctaaca ggattccaat aaacccgccg gtaatactga
    25021 ttattgttgc ttcggcaaca aactgaaaga tgatgtcatt tttgcgcgcg ccgattgctt
    25081 tgcgtaatcc aatctcgcta atacgttcca taacagaggc aagcattata ttcattatcc
    25141 ctatgccgcc aacaataagt gagatactgg caatagcgcc aagtacaata ttaaaaatat
    25201 ctttagtacg ttgttgttgg cgaagaagca gctctggaat gatgatctca aaatcttcct
    25261 cgccattgtg tcttcgttgc aacatacgtt gaattacctt agctgtggtt gtcatttttt
    25321 cgctgtcatg gatttgaacg atgagcttat ccaattgatt tgggttatcg gatgtttgtt
    25381 catcattatt tcggctcatc atggtagctc ttgtgataag agaacgatcg cgatagcgta
    25441 ataaaaccgt tttaatgggc gaaaagatgg aattattaaa ttcgctgacg cccatacttt
    25501 ttgtagctgt ttcgcttaca gtacgagatt tggtaatacc aatcacttcg agccagatac
    25561 ccccacattt tattttcttt ccaatggggt tttcctgttt aaaatatctg gcccgtatgt
    25621 cagggccaat gatacatact ggcgccgcat tttcgagttg ccaatttgaa aacatcttac
    25681 cctcaaataa ttcgaggtta tatacttcaa agaaatcggg ttctatgcct gagaaacgag
    25741 catttccacg tacccctgat ttaacgacaa aggtgttgta cactacttcg gggctgacct
    25801 tttgtacacc aggcactatt tctctaatgc ttgtggcatc ggcaagagta agccctggtg
    25861 aagatttttt atgaatctgt gatgctactt ctgtgtcatc agactgctgt tgtttttctt
    25921 tatcttctat ttttggagtg ataacgatat tattaacccc caccattttg atctgttcca
    25981 atatctcctg acgtgctccg ttgcctatag ccatcatagc tatcacggct gccacgccaa
    26041 atattatccc gag
//
LOCUS       DAOOAI010000016        25949 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_2485_length_25949_cov_4.594771, whole genome shotgun sequence.
ACCESSION   DAOOAI010000016 DAOOAI010000000
VERSION     DAOOAI010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25949)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25949)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25949
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2485_length_25949_cov_4.594771"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1558
                     /locus_tag="PK345_02265"
     CDS             <1..1558
                     /locus_tag="PK345_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HPX33876.1"
                     /translation="NWSRGQFAMSAMFHWVFVPLTLGLSILVAIMETIYLRTGDESWK
                     KMTKFWAKLFGINFAIGVATGIILEFQFGTNWSNYSWFVGDIFGAPLAIEGIMAFFLE
                     ATFVAVMFFGWNKVSKRFHLLSTWLVAIGANLSALWILVANAWMQYPVGTTFNPMTAR
                     NEMIDFWAVLFSPVAVNKFIHTVASGFVLASVFVVGVSSWFLLKKRDTLFAKRSIVIA
                     SVFGITTSLFLIWSGDESARQMADKQPMKFAAMEALWDGQANAPLVVIGLLQKPTPPK
                     TEGQLKEMEFAVKIEIPSLLSYMTYLDPNAYIPGIKDLVHGYTNPYTEKVEISYFEKM
                     ERGKEAIATLRNYKDALLREDYEALQEHKLKFEDPEWMESTFRYFGYGYYSESTVGGL
                     IPNIPIAFYSFHIMVALSGHFLALFIVMLVFTLKNRIEKLRWLLYVAIWTIPLAYLAS
                     QLGWVLAEMGRQPWVIQDLMPSIAAVSRVDAGSVQLTFWLFAITFTILLIAEVKILVA
                     QIKKGITGGH"
     gene            1560..2744
                     /locus_tag="PK345_02270"
     CDS             1560..2744
                     /locus_tag="PK345_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HPX33877.1"
                     /translation="MFETLSHFALQQYWWVIISLLASLLVFLFFVQGGQTLIFTLGKN
                     ELERAMLIGSLGHKWGLTFTTLVTFGGAFFASFPLFYSTSFGGAYWVWMIILFAFVIQ
                     AVAYEFRTKPNNFLGQRTYEAFLIINGAVGTILIGTAVGTFFTGSAFTVEKINLTNVL
                     LGGPTTISVWKGPAYGLEAALNVQNLSLGLAVFFLARVLGILYFMNSVDDENIFMRSR
                     KQLLINSAPFLVFFLLFLVLLLTSSGFAVIPDSVQNPMYWRIEMEPYKYLYNFLQMPV
                     VFVLFLAGVVLVLFGIVISLVKVGNTKGIWPSGIGTVLTVFSLFMVAGLNNTSYYPST
                     YDLQSSLTIMNSSSSKFTLTAMSYVSLMVPFVAAYIAYAWRALNRQKVTEAEIKSGDH
                     AY"
     gene            3007..6672
                     /locus_tag="PK345_02275"
     CDS             3007..6672
                     /locus_tag="PK345_02275"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPX33878.1"
                     /translation="MKNVLLLFVVAIMFMAKTGYCQDYKSLSDEDFANTPYWVEMMND
                     ESVNFFDVQRAFELYWKDREVTRSSGWKPFKRWEYLMMSRVNADGTRLPEDHNLKAFE
                     AYKAANPPHRFYSGNWSNLGPFLIPGSKGYNGLGRINAIEFHPTDPNTIYIGAPSGGL
                     WVSHDSGETWYTETDVLPTLGVSAILVDHQEPSVMYIGTGDRDAGDAPGVGVMKSFDE
                     GHTWQIYNNGMGNVKVGRMIMHPQNNQIILAAANSGIYKSINGGNNWQLKKNGDFREI
                     VYKTDDPNIVYASASGRFYRSIDGGESFDMISSGLPGGARAVIGVTPANPQLVYFVLT
                     NSDTFKGLYVSVDAGANFTVRATSPNLMGYDCNGGSGGQAWYNLDVAVDPFDDKVIFV
                     GGVNSYKSSDGGYTWQISSHWWGDCNVPAVHADLHVFEYSPHDGKLYAGNDGGLYWTA
                     DGGTNWTEISDGLAISQAYKIGQSPTVKDKVINGYQDNGTATYLGTSNWIPIIGGDGM
                     ECEVDHENHIYSYGTLYYGSIYRILNNTNAYKIAGNGSYGINESGGWVTPFLLHKGDA
                     KKMFVGYKNIWRGSNIRTNNPTWEKISNSLAGSNSSNMRELEQSPANYDILYAARADR
                     KLFRCDNVNGSNPVWTDLTQFLPDNILINDLEAHPTDENIVYMAMGSRAYKSSDKGKS
                     WTDITGSLPTVSLNSLAYYKISHEGLYAGTDLGIFYRDNFLDDWILYSDGFPFSGRVT
                     EVEIYHDPANPENDVIRAGTYGRGLWESDMFYDTPVADFTADQTLIPPFCELNFTDLS
                     AGVPTGWNWTFEGANPSNSNQKNPTGITYAEPGTFKVTLVVTNKAGTSEMVKEDYITV
                     SDQLMPVPDFSVDNTTACSNSVIHFNNLSQYCPTSFVWSFSPDNVIYLNGTNWNSPEP
                     VVRFTESGQYSVSLTVQNSNGTATVNRENYILVGGYTMPFYESFEEGFTRGWTLENPD
                     LRKTWQIIEPDYTPSGLNAAFMNHFDYYFMFERDRLISPPLNLSGVTEALLNFKHAYA
                     QRFSQVDSLLVKISTDCGNTWQTIYANGPDGNGGFETSLPTSLYFNPATEDDWCGGGY
                     GADCISLNISQYCNMPDVKIMFEAFNRIGNNLYIDDVSVEVLTSINEKSSRPESFNIY
                     PNPAKDEINVRFFTAESELDISIYSISGTEMIAEKVKNIKSGESVILKTDKLAPGAYF
                     VKVKGITISDSKLLIIK"
     gene            7074..10898
                     /locus_tag="PK345_02280"
     CDS             7074..10898
                     /locus_tag="PK345_02280"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33879.1"
                     /translation="MKTFTKMLFMALILTISTTLFAQKSIHSFVGNSAPDKMEMTRQV
                     LGYNNVTVPLTPEGMTRAVGDDCTNPIVISTLPFYDENQTTIGRGNTYDETCLNDYDS
                     GEDIIYRLEIASSVTINVHLDPLGTGYTGMALSDGCPLSSLCLAMSYDLSGNQEPHSF
                     TVSLDPGVYYIMVDTWAYPDNIEAFNLLITEVETVPNDFCESAIEIGEVINYPFSTIY
                     ATDDPSGETDGPEIWFNYKAGFTGYAVIDLCGSDYDTHLIVWDGVDCPPATMIIDVDD
                     GCGYFGLQSKGIIEVVEGNIYKLEIGGFSGDTGDGLLSIYEYEECNLECPPLGIDENE
                     PCGEDINGGCNMVTPAFTNVSNGNIICGNLWSESGTRDTDWFEVKLNGPGSIKMIVTA
                     EETLIFGLAGQNVLGVPGCENNTGFLSNYKVLSPCQEDSIWVINLPKGTYYLFAAPID
                     YDLHACPGFSYQAEFEIIELPSGFIKGDVIASDSGLPLEGVKITAGEAVTYTQSLGNY
                     ILELPVGTYTVEANGFAIAYSAVTKSDINVVLDDFTTVNFILDPLDAPELLTAVPGIE
                     QVELTWAEIGAKTDGSKTVMGDIFSKNDYIPGTTMNLDFVLSIYSPDFEWGEYAELVF
                     PEEFEIESVGDLNGVSGVIDGQKVSWNGVFYDSDTPQEIDFYVQVNVSGDATGPLVLL
                     YRVEGDGYGLDPHFFEGPVTVYEKNGDYVPTFNVYRRKIIPGTPTYFIPICYGVIGNY
                     YFDEIFLGGDEWCYYVTQIMPDKSESPASNVLCATPLIRPGSMCEHAIDYGEVNSPAV
                     NESLVRETDVRWFEFDVPYTMDIAVRLYNTNFDADVILYSDCDGTLLEDSDFCGGAEY
                     DIEKIYSLLPGGTYYAMVFGNEEEFGDFEIFITQVQVFTIREGWSGISTYMNPSGDLN
                     IASQLACFEENMIITIRPTPYGIWWPSQNINTLGDFSSMIGYKSKMEIEMTTVIYGTE
                     NPDKTVVLPANASYLPVRVTAPTSTDDLKNQLEGKLLILFDMYDPTAVIWPDGGLDNL
                     EYLVPDHAYMINMFVPAEYTYPMPSTAPAPNFVQPPARVKLEVWNEVINTGQPHFLSL
                     HVDALASLELGDYIGVFNSEGLCVGMVEYTSNENNHFIAAFGNDEYTEEKDGLDDGEL
                     MIFKVYRPSNGLVYALEVTYSDKMPDVDGLYKLYGMSMITGMKLGPTAIGEEQFNSLR
                     IYPNPTTGLFNISGLEGDFEVIITNAQGQQIMSLELNGQTTLDLTTQPKGVYFIRFTN
                     ENISKITKIVIK"
     gene            10980..11687
                     /locus_tag="PK345_02285"
     CDS             10980..11687
                     /locus_tag="PK345_02285"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: protein motif:HMM:NF008702.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HPX33880.1"
                     /translation="MMNLFFSKEISPPLFTFNPEESRHIVKVLRLTKHHRISITDGAG
                     RLYSGKIIDDNIKKCTVELDDTVVLEQKKDYKLHIAIALTKNLSRIEWFVEKAVEVGI
                     DEITPMLCRHSERIHFKADRLEKIAIAAVKQSQQLWLPTINELTSFDEIVTKTPSGEG
                     YIAFVDSASSGSFLKSVYSPGRNATILIGPEGDFSEEEISLALKKGFTSISLGKNRLR
                     TETAALFACFSFNLLNQ"
     gene            11695..12357
                     /locus_tag="PK345_02290"
     CDS             11695..12357
                     /locus_tag="PK345_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4159 domain-containing protein"
                     /protein_id="HPX33881.1"
                     /translation="MILKKNLLIFVLSISFLAANSQVASVQIALLKYRGGGDWYANPT
                     SLPNLIEFCNRNLRTNINPIPATVEVGSPDIFNYPFLHMTGHGNVVFSPQEAKNLRDY
                     LLAGGFLHIDDNYGMDKYIRPQLLKVFPELELIELPFSHPIYHQRYKFNDGVPKIHEH
                     DGKPPQGFGLFWEGRMILYYTYETDLGDGWEDQRVHGDSEEVRLKALQMGANIIQYVF
                     DR"
     gene            complement(12428..13114)
                     /locus_tag="PK345_02295"
     CDS             complement(12428..13114)
                     /locus_tag="PK345_02295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4412 domain-containing protein"
                     /protein_id="HPX33882.1"
                     /translation="MGKKNLFLTLIAALLLFHFPAVAQKKPFTGKVTYKIDLNMAGLP
                     EEVKGMMPKSMAMYIGESKVKTEIITAMSNQSAILDLNEKSHTTLFDVMGQKLAVKES
                     YDELTDQWPEDDYSIEITDDSKVIAGYKCKKVILKETDADGEQKEVGFAWFTDELKLH
                     ENFNFNNPIYKNLHGLLMEYEMDAGNNMNMKFTAIEVSSMKIKDSAFTPPEGYETITR
                     EELSKKFGSW"
     gene            complement(13128..14678)
                     /gene="lepB"
                     /locus_tag="PK345_02300"
     CDS             complement(13128..14678)
                     /gene="lepB"
                     /locus_tag="PK345_02300"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HPX33883.1"
                     /translation="MVTLLILILIFLFFPILLFKIFPKAGIESWKVFVPMLNYWEWNK
                     LNEKPIWWFIFLLVPFINIFMVFLMIVETAKAFGKNTLGQQALAVLFPYVYLPMLGID
                     SKEKYVKQANRPKYKKSAVREWVDAIIFAVVAATIIRTFLLEAYTIPTSSMEKSLLVG
                     DFLFVSKMAYGPKVPNTPIAFPFVHHTMPIFGTKSYVEWPKLPYYRFPGFGKVKLYDA
                     VVFNYPSGDTVVLERQNEDYYQIVRDVEVSYQRQYGERYKPGMGRSWVWSKYNVTARP
                     PDKRENYIKRCMAMPGDTIEIIDKIVYINGKEAYLPENLQYNYLVTATEQGFTQFELQ
                     KIMDELGITEGRAISPSRVILPLTSKMAEKLRTNPRIRNVELEIRPKGEHYSPIFPHD
                     PENFAWNEDNFGPLVIPRAGATVEINSKNIALYRKLIKIYDNNTLEEKDGKILINGQV
                     ASTYTFKQDYYWLMGDNRDNSADSRFWGFVPNDHVVGKAVFVWLSLDKNKGWFSGKIR
                     WKKCLRTI"
     gene            complement(14711..15454)
                     /gene="dapB"
                     /locus_tag="PK345_02305"
     CDS             complement(14711..15454)
                     /gene="dapB"
                     /locus_tag="PK345_02305"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HPX33884.1"
                     /translation="MKIAIAGYGKMGKTIESIAKDFGYKIVAIVDTEKDWETKNKEIG
                     QADVIIEFTMPSAAPSNIRRCFNLNIPVVSGTTGWHDQTEIIKKECLATNNTLLTAPN
                     FSIGVNIFFFLNRYLAKIMNRMYQYDLLLEEVHHQHKLDSPSGTAKLLADDLISLIER
                     KESWVNSKSNDTKILQVISTRLGEVPGTHLIRYFSDFDEIEIKHTAINRLGFARGALM
                     AAEWIKDKKGFFTFTDLMEDLFEIIKNDK"
     gene            complement(15460..16044)
                     /locus_tag="PK345_02310"
     CDS             complement(15460..16044)
                     /locus_tag="PK345_02310"
                     /inference="COORDINATES: protein motif:HMM:NF040144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5683 domain-containing protein"
                     /protein_id="HPX33885.1"
                     /translation="MKRQTDTFKISRVAIALLLSLSCYCVFPQINAQVTDTKKEHSPK
                     RATIYSAVLPGLGQAYNQKYWKIPIVYAGFGTLYYFIHTNGKEYRQFRDAFNIVASGD
                     TLNFPTNSYIIRYNANLDQLRAGRNYYRRNLELSYIITGALYILQIIDANVDAHLHDF
                     DISDDLSLSIEPYFPDQPLYAIKPVAGISIKLKL"
     gene            complement(16079..16960)
                     /locus_tag="PK345_02315"
     CDS             complement(16079..16960)
                     /locus_tag="PK345_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007749168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HPX33886.1"
                     /translation="MSKSKKSSLGRGLKAILQSPETDITSKDISGNYVVGAIANIALG
                     KIEANPFQPRNKFEEESLNELADSIREQGIIQPLTVRKLGYDKYQIISGERRLRAAKI
                     AGLADVPCYIRVANDEEMLELALVENIHRQDLNAIEIGISYQRFMEECNLTQEELSKK
                     VGKQRSTIANYIRLLKLPAEVQIALRENLISMSHARSIINLENRSDQIKLLKEILQNE
                     LTVREIEQKVKQAKKGKLKTSAKPLPEKFDLFSKKLSEELNTPVVLKKGSKGKGSIII
                     SFSSEEEFNRITASLLK"
     gene            complement(16962..17771)
                     /locus_tag="PK345_02320"
     CDS             complement(16962..17771)
                     /locus_tag="PK345_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPX33887.1"
                     /translation="MGKVIAIANQKGGVGKTTTAINLSAGLAVLEYKTLLVDADPQAN
                     STSGLGHDPRNIQNSIYECIIDDLDPSRSILHTSTPNLDIIPAHIDLVGAEIEMINMP
                     NREKMMRRVINKIKDDYEFIIIDCSPSLGLITVNALAAADSVIIPVQCEYFALEGLGK
                     LLNTIKIVQTRINPDLDIEGILLTMYDSRLRLAKQVVEEVRTHFQQMVFNTIIHRNTR
                     LGEAPSFGETIIMHDADSTGAINYLNLAREILQKNHLTKTAHNEISTDFLN"
     gene            complement(17953..18267)
                     /locus_tag="PK345_02325"
     CDS             complement(17953..18267)
                     /locus_tag="PK345_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3276 family protein"
                     /protein_id="HPX33888.1"
                     /translation="MEESFNKDNREDIFSLAIRAGKRTYFFDVKETRGGEKFFTITES
                     KRKFNSDSGKFFFEKHKIFLYKEDFEKFLNGLQGVIEFIETGKKPEIALDNQDEPESE
                     EL"
     gene            18530..19492
                     /gene="nusB"
                     /locus_tag="PK345_02330"
     CDS             18530..19492
                     /gene="nusB"
                     /locus_tag="PK345_02330"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HPX33889.1"
                     /translation="MLTRRHLRIKVLQALYAYFINNDIKLSIAEKNMTQSAERIYELA
                     IYQLSFIVEIVKFAAKRIEENKKKFYPTEDDLNPNTKFVDNLFIAKLTENRDYKKRKN
                     EYKINWIDHEEIIRRCYNDLRANDLYINYMTAGKQNFEDDKQLILKVFTNLLVDNESL
                     EYIYEEKSVYWANDIDLANYCVVRIISSINAQDDSLAKLPTLYNTEGKDDPDEDKNYM
                     IRLFHKAILHSKSYEKLIEEKASNWDFNRITLMDVILLKMGLAELIEFPTIPVKVTLN
                     EIIELAKLYSTPKSRVFINGILDKMSADLKAEGRIVKKGRGLIE"
     gene            19546..20985
                     /gene="rlmD"
                     /locus_tag="PK345_02335"
     CDS             19546..20985
                     /gene="rlmD"
                     /locus_tag="PK345_02335"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009316753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HPX33890.1"
                     /translation="MSRRRKNKKDLPVFERVQIYDAGAEGKAIARIDNMVIFVPFGAP
                     GDIVDIKVIGKRKSFYEGEIIGFHHYSDVRRSPKCEHFGLCGGCKWQHIEYGKQLYFK
                     QKQVVDSFERIGKIKIPAFQSIIPSESEYFYRNKLEFSFSNRKWLTDISSENINDTTT
                     NGLGMHLPRMFDRIVDLENCYLQKEPSNSIRLFLRDYGIKRNLTFYDSRNHHGFLRNV
                     TIRTTSTGQTMVIISFGYDDNNEIAELVNQLSTAFPQITSLFTVINQKRNDTIYDLAL
                     NPAHGKPYITEMLENLEFKIGPLSFFQTNTEQALVLYKIVRQFGSFKGNELVYDLYCG
                     TGTITCFVAKGVKSIVGIDNVPEAIDDARENSILNGIFNTAFVVGDLATTLNSDFVIK
                     YGAPDVIITDPPRSGMHPKVIKQILEILPEKIIYVSCNPATQARDISLLAEQYLLAEI
                     QPVDMFPQTHHVENVSLLIKKSLFSEQLV"
     gene            complement(20939..21469)
                     /locus_tag="PK345_02340"
     CDS             complement(20939..21469)
                     /locus_tag="PK345_02340"
                     /inference="COORDINATES: protein motif:HMM:NF013375.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HPX33891.1"
                     /translation="MKNVYLIGYMGAGKSTAGRQLARLLGYDFLDLDRAFEETYKISI
                     VDFFDKYDEDTFRLIERKMLENTFSLKSHVISTGGGTVCNGDNIRQINEHGISVYIRM
                     HPDSLFHRLRNSKRPRPRIINLKDAELKRFIEKDLEQRDSFYQMAHLQIKGEDLNFNA
                     LAKLIAPKIGISLLKD"
     gene            21839..22102
                     /locus_tag="PK345_02345"
     CDS             21839..22102
                     /locus_tag="PK345_02345"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPX33892.1"
                     /translation="MVLPKDVKPVEVFSGTAWQAEIIRDMLLDNEIESYLGDEIWGMD
                     APMKSAPDVVGSISVFVDREDLQDAKIVVERYYEEDFISEEEL"
     gene            complement(22197..22271)
                     /locus_tag="PK345_02350"
     tRNA            complement(22197..22271)
                     /locus_tag="PK345_02350"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(22234..22236),aa:Val,seq:gac)
     gene            complement(22628..23626)
                     /locus_tag="PK345_02355"
     CDS             complement(22628..23626)
                     /locus_tag="PK345_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HPX33893.1"
                     /translation="MNNEFLLHTADTDELQDKNYLDEYSRTVTGVVKATSKSIVNIKT
                     RKPPAGRPVQNIYPTFAMGSGFVISSNGIILTNHHVVNNIGEIIVTTPDGKEYPAKII
                     GVDAATDIALLKIEASGLNPLTFTDSDRLEPGQIAIAIGNPLGFQHTVTSGIVSALGR
                     TLRTQSGRLIDDVIQTDAAINPGSSGGPLLNSKGHVIGVNTAIIKGAQGICFSVSANI
                     AKYIATELLTKGKIKRAYLGIIGQNVKLPNDLINQWGLVKKTAVYIGGVDNKSGVENS
                     ALQKGDILIQLNDQVVGSIDDLHKLLTEKCINKELNLKIIRKGKLLELKVTAGELS"
     gene            23886..24419
                     /locus_tag="PK345_02360"
     CDS             23886..24419
                     /locus_tag="PK345_02360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HPX33894.1"
                     /translation="MLQTLITSKTRLRLLLKFFLNSNNISWLRDLESEFGESTNAIRV
                     ELNSLEKAGMLKSRMVGNKRIYRANSAHPLFDDIHNLLLKHTGIDQVVERIVNKIGGM
                     TEAWLVGSFAKGMDSPVIDILMIGDDIDQTYLTGLIEKVEEIINRRIRYLIVSCDEKD
                     IHLTQCPEAFQLWEKID"
     gene            24711..25751
                     /locus_tag="PK345_02365"
     CDS             24711..25751
                     /locus_tag="PK345_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005831335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HPX33895.1"
                     /translation="MSKNNTLLITGGTGSFGNAVLRRFLNDDFFKEIRILSRDEKKQD
                     DMRQHYKNDKIKYYIGDTRNKSAVTAAMRGADFVFQAAALKQVPSCEFFPIEAVKTNV
                     MGAENVLESAIELGVKSVVILSTDKAVYPINAMGMSKALSEKLMIAKSRGLNGTGQTF
                     SGTRYGNVMASRGSVIPLFIEQIKAGKPLTITDPEMTRFMMTLDEAVELVWFAFKNAE
                     NGDMFVQKAPAATIHVLAQALLELYKADNEIKIIGTRHGEKLFETLVNREEMAKAVDL
                     GNYYRIPADNRDLNYGKFFTEGQAKVSEVEDYTSHNTQRLDVEGMKKLLLKLPQIRKD
                     ILAEDISNIYEP"
BASE COUNT         7736 a         4715 c         5281 g         8217 t
ORIGIN      
        1 caattggtct cgaggtcagt ttgccatgtc ggcaatgttt cactgggtat ttgtacctct
       61 aacgcttggc ttgagtattt tggtagctat tatggaaacc atttatctgc gcactggtga
      121 tgagagttgg aagaaaatga caaagttctg ggcaaagctt tttgggatca actttgccat
      181 tggcgttgcg acaggtatca tactcgagtt tcagtttggt accaattggt cgaactactc
      241 atggtttgtt ggcgatattt ttggcgctcc gcttgctatc gaaggcatta tggccttctt
      301 tctcgaggct acttttgtag cggttatgtt ttttggttgg aacaaagtga gtaagcgttt
      361 tcacttgtta tcaacatggt tggtagctat cggtgcaaac ctttcagcgc tttggattct
      421 ggttgccaat gcctggatgc aatatcctgt gggtacaacc tttaacccaa tgaccgcccg
      481 taacgaaatg atagatttct gggctgtgtt attctccccg gtagctgtca acaagtttat
      541 tcatacagtg gcatcaggct ttgtattggc gtcagtgttt gttgttggag tctcctcttg
      601 gtttttatta aaaaaaagag atacactgtt tgccaaaaga agtatcgtga ttgcctccgt
      661 ttttggaata actacatcgc tatttttgat ctggtcgggc gatgaatcag cacgtcagat
      721 ggcagacaag cagccgatga aatttgctgc tatggaagca ctttgggatg ggcaggccaa
      781 tgctccgcta gtagttatag ggttattaca aaagcctacg cctcctaaaa cggaggggca
      841 acttaaggag atggaatttg cggttaagat cgaaattcct agcctacttt cttatatgac
      901 atacttagac cccaacgcat acatcccagg aataaaagac cttgtgcatg gttatactaa
      961 cccatatacc gaaaaggtcg agatctccta ttttgaaaag atggaacgcg ggaaggaggc
     1021 tatcgccaca ttacgaaatt acaaggatgc tttattgcgg gaggattatg aggcgcttca
     1081 ggagcataag ttaaagtttg aagaccctga gtggatggaa agcaccttta gatactttgg
     1141 ctacggatat tattctgaat ctacggtagg aggtcttata cctaacatcc ccattgcctt
     1201 ttattcgttc catattatgg tagctctgtc tggacatttt cttgcgcttt ttattgttat
     1261 gcttgtgttt acacttaaaa acagaattga aaaactgcgg tggctactct atgtggctat
     1321 ttggaccatt cctctggctt atctggcaag tcagttaggc tgggtattag ccgaaatggg
     1381 gcgtcaaccc tgggtgattc aggatttgat gccgtcaatt gcagcagtat cccgtgttga
     1441 tgctggtagt gttcaattga cattctggct gtttgccatt acattcacta ttctactgat
     1501 agcagaagtt aagattttag ttgcacaaat taaaaaaggg ataacaggag gccattaata
     1561 tgtttgaaac tctatcacat tttgcattgc agcaatactg gtgggtcata atttcgttgc
     1621 ttgccagtct gcttgttttt ctattctttg ttcagggtgg acaaacgttg atatttacct
     1681 taggcaaaaa tgaacttgag cgagcgatgc taattggttc gttaggacat aagtggggat
     1741 taacctttac tacattagta acttttggcg gagctttttt tgcatcattt ccattgtttt
     1801 acagcactag ctttggtgga gcatattggg tatggatgat cattttattt gcatttgtta
     1861 tacaggccgt ggcttatgag tttcgtacaa aaccaaacaa cttccttgga caacgcactt
     1921 atgaagcttt cttgataata aatggcgcag taggaacaat tttgattgga acagcagtag
     1981 ggacattttt tacaggttca gcgtttactg ttgaaaaaat aaaccttaca aatgttttat
     2041 tgggaggtcc aaccaccata tcagtgtgga aaggcccagc atacggattg gaagctgcct
     2101 tgaatgtcca aaatctttca ttaggattag cagttttttt tcttgcacgt gttttgggca
     2161 ttttgtattt tatgaatagt gttgacgatg aaaatatctt tatgagatca aggaaacaac
     2221 tccttatcaa ttcagcgcca ttccttgtct tcttcttgct tttcttagta ctgttgttaa
     2281 caagttccgg ttttgctgta atacccgatt cggttcaaaa tcctatgtat tggagaatag
     2341 aaatggagcc ttacaaatat ctttacaatt ttttgcaaat gcctgtggta tttgttttat
     2401 ttctggcagg agttgtttta gtgttgtttg gtatagttat ttcgcttgtt aaagtcggca
     2461 ataccaaagg tatctggcca tccggcatag gaaccgtact aacggtattt tccttgttta
     2521 tggttgcagg gttgaacaat acctcatatt acccttccac ttacgatctg caaagttcac
     2581 ttaccatcat gaatagctca tcaagtaaat ttactcttac tgctatgagc tatgtttctt
     2641 tgatggtgcc ttttgttgct gcttacatcg cttatgcttg gcgcgccctt aacagacaaa
     2701 aagtaactga agccgaaata aaatcaggtg atcatgctta ttaaataatt aaatactatc
     2761 ataaattatg tactggaaag agattttgca ttttctgagc cttccgctgt tagttgttgt
     2821 aacctacagg atcattctgt tctatttgaa aaagctagat cgaagaatag gtgaaattaa
     2881 taacgaataa tcctgaaatt caatagatca atcttaacac tttatcaatc gtactggtga
     2941 aaaaggattg agataggtat ttaggaaaat agtgtatttt tgttcggttt tataaaatct
     3001 ataaatatga aaaatgttct acttcttttt gtggttgcca ttatgttcat ggcgaaaact
     3061 ggctattgtc aggattacaa atcactcagt gatgaggatt ttgccaatac tccttactgg
     3121 gttgaaatga tgaatgatga atcggtaaat ttctttgatg ttcaacgtgc ttttgagctt
     3181 tactggaaag atcgtgaagt tacaagatct tcgggttgga agccatttaa aagatgggaa
     3241 tatttgatga tgtcaagagt gaatgctgat ggcacacgct tgcccgaaga ccataatctg
     3301 aaagcatttg aggcttataa agcggccaat ccgccacatc gcttttattc aggtaactgg
     3361 tctaaccttg gccctttttt gataccagga agtaaaggtt acaacggcct tggccgaatc
     3421 aatgcaatag agtttcatcc taccgaccca aacacaatat atattggcgc tccttcaggt
     3481 ggattatggg tttcgcatga tagcggtgaa acttggtaca ccgaaacaga cgttttgcct
     3541 accttaggag tttcagcaat tttggtagat catcaggagc cttcggtgat gtatattgga
     3601 accggcgacc gtgacgctgg agatgcgcca ggtgtaggtg ttatgaaaag ttttgatgaa
     3661 ggccatacat ggcaaattta taataatgga atgggaaacg ttaaagttgg ccgaatgata
     3721 atgcacccgc agaataatca gataatatta gcggcagcaa attcaggaat ttataaaagt
     3781 ataaatggcg gaaataattg gcaactgaag aaaaatggtg atttcagaga gatagtctat
     3841 aaaaccgacg atcctaatat agtatatgca tctgcatcag gaaggtttta tcgctcaatt
     3901 gatggagggg agagttttga tatgatttca tccggactgc ctggtggagc gagagctgtg
     3961 attggagtta ctccggcaaa tcctcaatta gtctatttcg ttttaacaaa tagcgatact
     4021 ttcaaaggcc tctatgtttc ggtagatgcc ggcgccaatt ttaccgtacg cgctacttca
     4081 cccaacttaa tgggctatga ttgtaatggt ggtagtggtg ggcaggcatg gtacaatctt
     4141 gatgttgccg tagatccttt cgacgataaa gtgatttttg ttggtggggt aaatagttat
     4201 aaatcaagtg atggcggcta tacatggcag atcagttcgc actggtgggg cgattgtaat
     4261 gttccggctg tacatgctga tttgcatgtg tttgaatata gtccacatga tggaaagtta
     4321 tatgcaggta atgatggagg cttatactgg acggcagatg gaggaactaa ctggactgag
     4381 atttctgatg gcctggcaat tagtcaggca tacaagatag gacagagccc cacagtgaaa
     4441 gataaggtaa ttaatggata tcaggacaac ggaacagcaa cctaccttgg aacatcaaac
     4501 tggataccta taataggcgg agatggaatg gaatgcgaag tggatcacga aaatcatatc
     4561 tatagctacg gaacacttta ttatggctct atctacagga ttttaaataa tacaaatgca
     4621 tataaaattg ctggaaatgg atcatacggt ataaacgaat caggtggttg ggtaacacct
     4681 ttcttgttac acaaaggcga tgcaaaaaag atgtttgtag gttataaaaa tatttggcga
     4741 ggaagtaaca tcagaaccaa taatcctact tgggagaaaa tatcaaatag tttagcggga
     4801 agtaatagct ccaatatgcg tgaattagaa cagtcgcccg ctaactatga tatactttat
     4861 gctgcacgtg cagacaggaa attgttcaga tgcgataatg taaacggatc aaatcctgtg
     4921 tggactgacc tgacacagtt tttgcccgat aatattttga tcaatgatct ggaagctcac
     4981 cctacagatg aaaatattgt ttatatggct atgggtagca gggcatacaa atcatccgat
     5041 aagggaaaat catggacaga cataaccggc tctcttccaa cagtatcgtt aaatagcctg
     5101 gcatattata aaatcagcca tgaaggatta tatgcaggca ctgatctggg aattttttat
     5161 cgcgataatt ttcttgacga ctggattttg tatagcgacg gcttcccttt tagtgggcgt
     5221 gttaccgagg ttgagattta tcacgatccg gcaaatcctg aaaatgatgt tattagagcc
     5281 ggcacttacg ggcggggatt atgggaatcc gatatgtttt atgatactcc ggtagccgat
     5341 ttcacagccg atcaaacatt gatcccgcca ttctgcgagt tgaattttac tgatctatca
     5401 gcaggagtac ctaccggatg gaactggacg ttcgagggag ctaatccttc aaattcaaac
     5461 cagaaaaatc ctactggtat aacttatgct gaaccaggaa catttaaagt tacattggta
     5521 gtaacaaaca aagccggaac ttctgaaatg gtaaaagagg attacattac tgtaagcgac
     5581 cagcttatgc ctgtaccaga cttttcagtt gataatacta ccgcatgttc aaattctgtt
     5641 atccatttta ataacctctc tcaatactgc ccaacatcct ttgtttggag cttttcgccc
     5701 gataatgtta tctatctgaa tgggacaaat tggaatagcc ccgaacctgt tgttcgtttt
     5761 accgaatctg gccaatattc cgtttcgtta acagtacaaa acagtaatgg tactgcaact
     5821 gttaatcgtg aaaactacat acttgttggt ggatatacca tgccgtttta cgagtcattt
     5881 gaagagggtt ttacacgtgg ttggacatta gaaaaccccg atctccgtaa aacctggcaa
     5941 attattgaac cggattacac gccaagcggc ctcaatgcag catttatgaa tcattttgat
     6001 tactatttta tgttcgaaag ggatcgtttg atatcgcctc cactaaatct cagcggtgtt
     6061 accgaagctt tactcaactt caaacatgct tatgcacaga gattctctca agtagattca
     6121 ttgttggtaa agatctctac tgattgcggt aacacatggc aaacaattta tgccaacggg
     6181 cccgatggta atggcggatt tgaaacttcc ctaccaacct cgctctactt caatccagct
     6241 accgaagatg attggtgtgg gggcggttat ggcgcagatt gtatcagcct caatatctcg
     6301 caatattgta atatgccgga cgtgaaaatt atgttcgaag catttaaccg tattggtaat
     6361 aatctttata ttgatgatgt ttctgtggaa gtattgactt ccattaatga gaaatccagt
     6421 cggcctgaga gctttaatat ttatccaaac cctgcaaagg atgaaataaa cgtacgattt
     6481 tttactgctg aatccgaatt ggatatttca atttattcta ttagtggaac agaaatgatc
     6541 gcagaaaaag tgaaaaatat taagtccggg gaatccgtca ttttaaaaac agataaattg
     6601 gcgccgggag cctattttgt aaaagttaaa ggaataacaa tatcagattc taaattgtta
     6661 ataatcaagt aaatatttaa attaagcttg tttagtagaa tagtattggc taatgaattt
     6721 ttaattaata ggaggacttt tttatttcat tttcaaaatt tctttgcaca tgaacctgct
     6781 cggaaaatac tcttttagca gcttgggcat ttatgttttg atttagtaaa ttgtagagaa
     6841 tcaacttact aagctggata aaattggatt aatatgttaa atatttaaaa aaaatcaaat
     6901 tttctttatt caaaacaaaa ttatatttat tattgcaatt aatttcagtt ttttattaat
     6961 taatcatctt gtttaaaata gaaatattat ttttgcagtc aaaataattt agaaccttat
     7021 taatcacatt aatttgaaat aatttaacaa actattaatt taaatcataa atcatgaaaa
     7081 cttttacaaa aatgcttttt atggcgttga ttcttaccat ttcaacaact ctttttgcac
     7141 aaaaaagtat tcacagtttt gttggtaatt cagcgcctga taaaatggaa atgacccgtc
     7201 aggtattagg ctataataat gtaaccgtac cgcttacccc cgaaggaatg acaagggctg
     7261 taggcgatga ttgcaccaat cctattgtga taagtacgct tcctttctac gatgaaaacc
     7321 aaaccactat tggtagagga aatacttatg atgagacttg tcttaatgat tatgatagtg
     7381 gtgaagatat catttatcgt ttggagattg catcatcagt tacaattaat gtgcaccttg
     7441 atccactagg tacaggctat accggcatgg cgctctccga tggttgcccg ctctcttccc
     7501 tttgtcttgc catgagttat gacctttctg gtaaccaaga gccacatagt tttacagtat
     7561 ctcttgaccc tggtgtctat tatataatgg tggatacttg ggcatatccg gataatattg
     7621 aggcttttaa tctcttaatc accgaggtgg aaactgtacc gaacgatttt tgcgaaagtg
     7681 caatagaaat tggcgaagtt attaattatc ctttttcaac tatttacgca accgatgatc
     7741 catctgggga aaccgatggt ccggaaatat ggtttaatta taaagccggt tttacaggct
     7801 atgcggtaat tgacctttgc ggaagtgatt acgacactca tcttattgtt tgggatggcg
     7861 ttgattgccc acctgctacc atgattatag atgtagatga tggttgtggt tattttggat
     7921 tacaatcaaa agggataata gaagtagtcg aaggcaacat ttataaatta gaaataggtg
     7981 gcttttcagg tgatactggc gatggtttgc tttcaattta tgaatatgag gagtgcaatt
     8041 tagaatgccc accacttggt attgatgaaa atgaaccttg cggagaggat atcaacggtg
     8101 gctgtaacat ggttacacct gcattcacta acgttagcaa tggtaacata atttgcggta
     8161 atctttggag tgaatctgga acacgtgata ccgattggtt tgaggttaaa cttaatggac
     8221 caggaagcat taaaatgata gtaactgctg aagagacttt aatatttggc ctcgcaggtc
     8281 agaatgtttt aggtgtccct ggctgtgaaa ataacactgg ttttctctcc aattataagg
     8341 tattgtctcc ttgccaagaa gattctattt gggtaattaa tttaccaaag ggtacttatt
     8401 acctgtttgc tgctccgata gattatgacc tccatgcttg cccaggattc agctatcaag
     8461 ccgaatttga aattatagag ttgcccagcg gatttattaa aggagatgtg atcgcaagtg
     8521 attcaggtct tccgttagaa ggagtaaaga ttactgctgg agaagctgtt acctacacac
     8581 aatcacttgg taattacatc cttgagctgc ctgttgggac atataccgta gaagctaatg
     8641 gattcgcaat agcatatagc gctgtaacaa agtcggatat taatgttgtg ttggatgatt
     8701 ttacaacagt aaattttata cttgacccac tggatgcccc cgagcttttg accgcagttc
     8761 caggaatcga acaggttgag ctgacatggg ctgaaatagg agcaaaaacc gatggaagta
     8821 aaacagtgat gggcgacatc ttttcgaaga atgattatat acccgggacc acaatgaacc
     8881 tcgattttgt attgtcaatt tactcgccag attttgagtg gggagaatat gcagagttgg
     8941 tatttcctga agaattcgaa attgaatctg ttggcgatct taatggagtt tctggtgtaa
     9001 ttgatgggca aaaggtttca tggaacggtg tcttttatga ttcagacacg ccacaggaga
     9061 ttgattttta tgttcaggtt aatgtgtctg gagatgcaac aggtccgttg gtattgttat
     9121 accgagttga aggtgatggt tacggtttag atcctcattt cttcgaaggc cctgtaacgg
     9181 tatatgaaaa gaatggcgat tatgttccta cttttaatgt ttaccgtcgc aagattatcc
     9241 ctggaacccc cacttatttt ataccgattt gttatggagt aataggtaac tattattttg
     9301 acgaaatatt ccttggtggc gatgagtggt gctattatgt aacgcaaatt atgcctgata
     9361 aatcagaatc tccggcatca aatgttctat gtgctactcc tttgattagg cctggttcaa
     9421 tgtgtgaaca tgctattgat tacggtgaag taaacagccc tgctgtgaat gagtctcttg
     9481 ttcgtgaaac tgatgtgcgc tggtttgagt ttgatgtacc ttatactatg gatatcgccg
     9541 ttagacttta caatacaaac tttgatgctg atgttatatt atattccgat tgcgatggta
     9601 cattattaga agactcagat ttttgtggtg gagctgaata cgatattgaa aaaatatact
     9661 cattattgcc cggaggcaca tattacgcaa tggtttttgg taacgaggaa gagtttggcg
     9721 attttgagat tttcataaca caagttcagg tgtttacaat tagagaagga tggagtggaa
     9781 tatccacata catgaatcct tccggcgacc tgaatatagc tagtcaatta gcttgtttcg
     9841 aagaaaatat gataattact atccgtccga ctccgtatgg tatttggtgg ccttcacaga
     9901 atattaacac attaggcgat ttttccagca tgattggtta taaatcaaaa atggaaattg
     9961 aaatgactac agtaatttac ggaacagaaa atcctgacaa aactgttgtc cttcctgcca
    10021 atgcaagcta tttgccagtg agagtaacag cgccaacctc tactgatgat ctgaaaaatc
    10081 aactcgaagg aaagttattg atcctttttg atatgtacga tcctaccgca gtgatatggc
    10141 ctgatggagg tctcgataat cttgagtatc ttgtgcctga ccatgcatac atgattaata
    10201 tgtttgtgcc tgctgaatat acctatccaa tgccatccac agcgccagct cctaacttcg
    10261 ttcagccgcc ggcgagagtg aaattagaag tttggaatga ggtaataaat accggacaac
    10321 cacatttcct ttcacttcac gtagatgccc ttgcctctct ggaattggga gattacatcg
    10381 gtgtgtttaa cagcgaaggt ttgtgtgtag gaatggtaga gtatacatca aatgagaaca
    10441 atcactttat tgctgctttc ggcaacgatg aatatactga ggaaaaagat ggattagatg
    10501 atggagaact aatgattttc aaggtttaca gaccatccaa tggacttgtc tatgcacttg
    10561 aggtaactta tagcgataaa atgcccgatg ttgatgggtt gtataaatta tatggaatgt
    10621 cgatgattac aggaatgaaa ttaggcccaa ccgctattgg cgaagagcaa tttaactctt
    10681 tgaggattta tccgaatcct accacagggt tgtttaacat ttctggatta gaaggcgatt
    10741 ttgaggtgat aattaccaat gctcaaggcc aacagatcat gagtttagag ttgaatggtc
    10801 aaactactct tgatttaaca acacagccaa aaggtgttta ctttattaga tttacaaacg
    10861 aaaatatttc aaaaattaca aaaattgtaa ttaaataata tctgttgtct tttggtattt
    10921 gaaagcccgt tctatgtttt agagcgggct ttttcataaa atatcatatt gttaaatgaa
    10981 tgatgaactt attcttttct aaagaaattt caccgccact ttttactttt aatcccgaag
    11041 agtcccgaca tatcgttaaa gtattgaggt taaccaaaca ccatcggatt tctattactg
    11101 atggcgccgg taggctatat tcaggaaaaa ttattgatga caacattaaa aaatgtaccg
    11161 ttgaattaga cgatacggta gttttagaac agaaaaaaga ttataagttg catatagcaa
    11221 tagccttgac caagaacctt tcacgtatcg aatggtttgt tgaaaaggct gttgaagttg
    11281 ggattgacga aattacgcca atgttgtgcc gccactccga aaggattcat tttaaagctg
    11341 acagacttga aaagatcgct attgctgctg ttaaacagtc gcagcaatta tggcttccta
    11401 ctatcaacga attgacctca tttgatgaaa tagtaactaa gaccccatcg ggcgaaggtt
    11461 atatagcttt tgttgatagc gcttcaagcg gctccttttt aaagtcagtc tattcaccag
    11521 gccgaaatgc gacaatactg ataggaccgg agggggattt ttctgaggaa gagatctctt
    11581 tagctttaaa gaaaggcttt acttcaataa gccttggaaa aaatcgttta agaaccgaaa
    11641 cagcagcgct ctttgcctgt ttttcattta atcttttaaa tcagtaaaca aaatatgatt
    11701 ttaaaaaaga atcttttaat ttttgttttg tcaataagtt ttcttgctgc caacagtcag
    11761 gtcgcttcgg ttcagattgc tcttttaaaa tatcgtggcg ggggcgactg gtatgccaac
    11821 cccacttcat tgcctaactt gattgagttt tgtaaccgaa accttagaac caacatcaat
    11881 ccaataccgg caacagttga ggttggaagc cccgatattt ttaattatcc atttttacac
    11941 atgaccggac atggaaatgt tgtgttctct ccgcaggaag ctaagaacct tcgtgattat
    12001 cttctggccg gcggattttt acatatagat gataactatg gaatggataa atacataagg
    12061 ccgcaattgc taaaggtgtt tcctgaactg gaacttattg agctgccatt ttcgcatcct
    12121 atttatcatc aaagatataa attcaacgac ggcgttccta aaatccatga acacgatggt
    12181 aaacctccac aaggattcgg attgttttgg gagggcagga tgatattata ttacacttac
    12241 gaaacagacc ttggcgatgg ttgggaagat cagcgtgttc atggcgattc ggaagaggta
    12301 agactcaaag cgctgcaaat gggagccaat atcattcaat atgtttttga taggtagcct
    12361 atggctggca atttatgaat taacctcctt attggtacta aactttgctt tgttttaaag
    12421 tattaatcta ccaactgcca aatttcttag ataactcttc gcgagttatc gtttcgtaac
    12481 cttcaggggg agtaaaagcc gaatccttga ttttcatgct ggaaacttca atggcagtaa
    12541 atttcatgtt catattattg ccggcatcca tttcatattc cattaatagg ccatgaagat
    12601 ttttataaat gggattatta aaattgaagt tttcgtgtag ttttaattca tcagtaaacc
    12661 atgcaaaacc gacttctttt tgctcaccat cggcatctgt ctctttaagt ataaccttct
    12721 tgcatttgta acctgcaata acttttgaat catcggttat ttctatggag tagtcatctt
    12781 caggccattg atcagtaagt tcatcgtatg attcttttac agccaatttc tgacccataa
    12841 cgtcgaacag cgtagtgtgg cttttctcat tcagatcaag aatggcgctt tgattgctca
    12901 tggcggtaat aatttctgtt ttcaccttac tttcgccaat atacattgcc atacttttag
    12961 gcatcatccc ttttacctct tcaggaagtc cggccatgtt aagatcaatt ttgtaggtta
    13021 cctttccagt aaatggcttt ttctgtgcaa ctgccggaaa atgaaaaagc aatagcgctg
    13081 ctattaatgt taaaaagaga ttttttttac ccataatatt tatcatacta aatggtacgc
    13141 aaacattttt tccatcttat ttttcctgaa aaccagcctt tatttttatc taaagagagc
    13201 caaacaaaaa ctgcttttcc aactacatgg tcatttggca caaaccccca gaatcttgaa
    13261 tcggccgagt tgtcgcgatt atcgcccatg agccagtaat aatcctgttt aaatgtgtat
    13321 gtagaagcga cttgcccatt aatcaaaatc tttccatctt tttcttcaag ggtattgtta
    13381 tcataaattt ttataagctt acgataaagg gctatgtttt tcgaatttat ctcaacggta
    13441 gctccagccc tgggaataac caatggtccg aagttatctt cattccacgc aaaattttca
    13501 ggatcatgtg gaaagatagg tgagtagtgt tctccttttg gtcgtatttc taactctacg
    13561 tttctgattc ttgggttggt tcttagcttt tctgccattt ttgaggtgag tggtaagatc
    13621 accctgcttg gcgatatggc tcttccttca gttatgccaa gctcgtccat tattttttgt
    13681 agttcgaatt gcgtaaagcc ctgctcagta gcggttacca gatagttgta ctggaggttt
    13741 tcgggtaggt aagcctcttt tccatttata taaacaattt tatcaattat ctcgatagtg
    13801 tcgcccggca ttgccataca acgttttata taattttccc ttttgtcggg agggcgggct
    13861 gttacgttat acttcgacca tacccaacta cgtcccatac cgggtttata tctctcgcca
    13921 tactgacgtt gataactgac ctcaacatcc cgaacaattt gataataatc ttcattttgc
    13981 cgctcaagca ctacagtatc gccgcttggg taattaaaaa cgacagcatc gtaaagtttt
    14041 accttaccaa atcccggaaa acggtaatag ggcagtttag gccattccac ataggattta
    14101 gtgccaaaaa taggcatcgt gtgatgaaca aaaggaaaag caattggagt attaggaact
    14161 tttggcccgt aagccatttt gctgacaaac aggaaatcgc caaccaataa ggatttttcc
    14221 atcgaggagg tcggtatggt ataggcttct aacaggaaag tgcgaatgat agtagcagct
    14281 acaacagcaa agataatcgc atcaacccat tcgcgaacag cggacttctt gtatttagga
    14341 cgattggctt gtttaacata cttctcctta ctgtcaattc caagcatagg aaggtaaacg
    14401 tatggaaata ataccgcgag cgcctgctga ccaagtgtgt ttttaccaaa tgcttttgca
    14461 gtttccacaa tcatgaggaa aaccataaaa atattgatga aaggtactaa gagaaagata
    14521 aaccaccata taggcttctc gttcagcttg ttccattccc agtaattcaa cattggaaca
    14581 aacactttcc acgattcaat accggctttt ggaaatattt tgaatagtaa aattggaaag
    14641 aaaagaaaga ttaaaatcaa aataagtaat gtaaccatcg gttaaaatgt taatttaagt
    14701 atgatcaact ctatttatca ttttttatta tttcaaacaa gtcctccata agatcggtaa
    14761 atgtaaaaaa tccttttttg tcttttatcc attcggcggc catcaatgcg ccacgagcaa
    14821 atccaaggcg gttgatagca gtatgtttga tttctatttc atcaaaatcc gaaaagtaac
    14881 gaatcaggtg agtacccgga acttcgccaa gtctggtaga gatcacctgc aaaattttgg
    14941 tatcattact tttactgtta acccatgatt cttttctttc aattaagctg ataaggtcgt
    15001 cggctaaaag ttttgccgta ccgctgggtg agtcgagctt gtgttgatga tgaacctcct
    15061 caaggagcaa atcatattga tacattcgat tcataatttt ggctaaatag cggttcaaga
    15121 agaaaaagat gtttactcca atactgaaat taggagcggt caaaagggta ttatttgtag
    15181 caagacactc ttttttgatt atctctgttt ggtcgtgcca acctgtagtg ccactaacaa
    15241 ccggaatatt gaggttaaaa caccggcgta tgtttgatgg ggcagccgaa ggcattgtaa
    15301 actcgataat aacatcagct tggccaattt ctttgttttt tgtctcccaa tctttttcag
    15361 tatcaacaat tgccacgatt ttatatccaa aatccttagc tattgattca attgtttttc
    15421 ccattttccc atatccggca atagcaattt tcataagtgt taaagtttta gttttattga
    15481 tatccctgct acaggcttta ttgcatatag aggttgatcc ggaaaataag gctctatgct
    15541 taagctcaga tcatcgctga tgtcgaaatc gtgtagatgt gcatccacgt tggcatcaat
    15601 gatttgcagg atgtagagcg cgccggtaat gatataactg agttccaggt ttcggcggta
    15661 atagttgcgc ccggcccgca actgatcaag gtttgcattg taacgtatga tatagctgtt
    15721 ggttgggaaa tttaatgtat caccacttgc tactatatta aaagcatctc tgaactggcg
    15781 gtactctttg ccgttggtgt gtatgaaata gtataaagtg ccaaagccgg catatacgat
    15841 cggtatcttc cagtatttct gattgtaagc ctggccaagg ccgggaagca cagcagagta
    15901 tatggtagcc cttttgggcg aatgttcttt ttttgtgtcc gttacctgcg cgttaatttg
    15961 tggaaacaca caatagcatg acaagcttaa caggagcgct attgccactc ttgaaatttt
    16021 gaatgtatca gtttgtcgtt tcaagaggct cagtaattaa tttagacaat aattggtttt
    16081 actttaataa cgatgctgtg attctgttga actcttcctc cgaggaaaaa ctaataatta
    16141 ttgagccctt tcctttgctg ccctttttta aaacaacagg tgtattaagt tcttccgaga
    16201 gttttttgct aaagaggtca aacttttcgg gcaaaggttt ggcgctggtt tttagtttcc
    16261 ctttttttgc ctgttttacc ttttgttcta tttcgcgtac tgtaagttca ttttgaagga
    16321 tctctttcaa tagtttaatt tgatcgcttc tattttccaa gttaatgata gagcgtgcat
    16381 ggctcatgct tatcaaattt tcgcgaaggg caatctgcac ttctgccggt agtttcaaga
    16441 gacggatata attggcaata gtggaacgct gtttgccaac ttttttgcta agttcttcct
    16501 gtgttaggtt gcactcttcc atgaaccgtt gatagcttat tcctatctca atagcattca
    16561 aatcctgacg gtggatattt tcgactaacg ccagttccaa catctcctca tcatttgcca
    16621 cacgtatgta acatggcaca tcggcgaggc cggcaatctt agcggcacgt agccgccgct
    16681 cacccgaaat gatctgatat ttgtcatatc caagcttacg aaccgtcaga ggttgaataa
    16741 taccctgctc gcgtatggaa tcggctaatt cgttgagcga ctcttcttca aacttgtttc
    16801 gaggctgaaa ggggttggct tctatctttc ctaatgcgat gttagcaata gctcccacaa
    16861 cgtagttgcc tgaaatatct tttgaagtga tatcagtttc aggactttgt aaaatagctt
    16921 taagccccct gcctaacgag ctttttttgg atttactcat gttaatttaa aaaatctgtg
    16981 cttatttcgt tgtgagcagt ttttgttaag tgatttttct gaagtatctc ccttgccaga
    17041 ttcagataat taattgcgcc ggtagaatca gcatcatgca taatgattgt ctcaccgaaa
    17101 cttggagctt cacccaacct tgtattgcgg tggatgatag tgttaaacac catttgctga
    17161 aaatgagtcc ttacctcttc aacaacctgt ttggctaatc ttagccttga atcgtacata
    17221 gtaagcaaaa tcccttcgat atccaaatca ggatttatcc tcgtctgtac tattttgata
    17281 gtgttgagca acttgcctaa tccttccaaa gcaaaatatt cgcattgcac ggggatgatt
    17341 actgaatctg ctgctgcaag agcgttaaca gttatcagtc ctaatgaggg tgaacagtca
    17401 ataattataa attcgtaatc gtcctttatt ttattgataa ccctcctcat catcttttcg
    17461 cggttaggca tgttgatcat ttctatttcg gcgccaacaa gatcaatatg tgcgggtatg
    17521 atatctaaat ttggagttga agtatgtagt attgatctcg atgggtccaa gtcgtcaatg
    17581 atacattcgt aaatgctatt ttggatgtta cggggatcat gccccagacc tgatgtagag
    17641 ttggcctgag ggtcagcgtc aacaagaagg gttttgtact caagaacggc caaaccagcg
    17701 cttaaattaa tggctgttgt cgttttacct acgccccctt tttgattggc tatagcgatt
    17761 acttttccca taaattccac acatttttat tgtgtgcaaa attaagataa taaacgcacg
    17821 caaaagtgga agttttaaaa agtaatcaac acaatgcctt agacatgcat tttcaaaact
    17881 ttttatgtga atggataaga tgccgtgatt gtgagacctg agcttactct cataaactca
    17941 tataatcatc ttttacagct cctccgactc gggttcatct tgattatcta atgcgatttc
    18001 aggctttttg cccgtctcaa tgaattcgat gacgccctgt aacccattca aaaacttttc
    18061 aaaatcttct ttgtaaagga aaatcttatg cttttcaaaa aagaactttc ctgaatcgct
    18121 attaaatttt cgcttactct cagtaatggt gaaaaacttt tcgccacctc tggtttcctt
    18181 tacatcaaaa aagtaagtcc ttttgcctgc tctgattgcg agagaaaaaa tatcctccct
    18241 gttgtccttg ttaaaagatt cttccatttt atacaatttt gagatttagt gtgaaccaaa
    18301 aatttccagt acaacgccag tgcgatacac cgtttgacta ttcttattga tttattatta
    18361 agagtatcaa accgacatca tcttttagtt tttggctttt atattgcaaa agtatgtata
    18421 aatccttacc gtcaaaaaat taaataataa aattttaaaa aaaaagtagt cgtaaaatat
    18481 ttaattttgc acctttaaaa cacttcttta gaaactgttc ttacatttta tgcttaccag
    18541 aaggcattta agaataaaag tcttacaggc tctctatgct tactttatca ataatgatat
    18601 caagttaagc attgccgaga aaaatatgac tcaaagcgct gagcgtatct acgagcttgc
    18661 catttatcag ctttcattca ttgttgaaat agtgaagttt gccgctaaaa gaatcgaaga
    18721 gaacaaaaaa aagttttacc cgaccgaaga cgacctcaat cccaatacca agtttgttga
    18781 taatcttttc attgcaaaac ttaccgaaaa cagggattat aaaaaacgca aaaatgaata
    18841 caagataaac tggattgatc acgaagagat tatcagacgc tgctataatg acttacgtgc
    18901 aaacgacctt tacatcaatt acatgactgc cgggaaacaa aactttgaag atgataaaca
    18961 gttgatatta aaggttttta ctaacttact cgttgataat gaatcacttg aatatattta
    19021 cgaggaaaaa agtgtctatt gggccaacga tattgatctg gccaactatt gtgttgttag
    19081 gataatcagt tcgataaatg cgcaagatga tagtctggct aagctaccta cactctacaa
    19141 cactgaaggc aaggacgatc ccgatgagga taagaattat atgatcaggc tttttcataa
    19201 agctatttta catagcaaaa gctacgaaaa actgattgag gagaaagcaa gcaattggga
    19261 tttcaatcgt atcacgctga tggatgtcat attgcttaaa atgggattag ccgaacttat
    19321 tgagtttcca accattccgg ttaaagtaac cttaaatgaa attattgaat tggcaaagct
    19381 ttatagcaca cctaaaagcc gtgttttcat caatgggata ttggataaga tgagcgccga
    19441 cctgaaagcc gaagggcgaa tagtgaaaaa gggtagggga ctgattgaat aattctttag
    19501 gccattcttg cttttcttta ccttaaaaca agttaataga ggattgtgtc aaggagacga
    19561 aaaaataaaa aagacttacc tgtttttgaa agggttcaaa tttacgatgc aggtgctgaa
    19621 ggtaaagcca ttgcacggat agataatatg gtgatcttcg tgccttttgg cgcaccgggg
    19681 gatatcgtgg atattaaagt gataggtaaa agaaaatcgt tttatgaagg agaaataatt
    19741 ggttttcacc actactccga tgtaaggaga agtccaaaat gcgagcattt tggactgtgc
    19801 ggaggatgca aatggcaaca tattgaatat ggcaaacagc tctattttaa acaaaaacag
    19861 gttgtggata gcttcgaacg tattggaaag atcaagatac ctgcctttca atcaattata
    19921 ccatccgaat cggagtattt ttaccgtaat aagcttgagt tctcattttc aaatcggaaa
    19981 tggctgaccg atatttcaag tgaaaatatc aatgatacca ctactaatgg gcttggaatg
    20041 cacttgccca gaatgtttga tcggatcgtt gacttagaaa attgctattt gcaaaaggag
    20101 ccatctaatt ctatccgtct atttttacgt gattatggca tcaaaagaaa tctgaccttt
    20161 tatgattcgc gaaatcatca tggttttttg cgaaatgtta ccattcgcac tacttcaaca
    20221 gggcaaacaa tggttatcat ctcttttggt tatgatgaca acaacgagat tgctgagctt
    20281 gttaaccaac tttccacggc tttcccccag atcacttcac tttttaccgt gataaaccaa
    20341 aagcgtaacg acaccattta tgatcttgct cttaatccgg ctcatggaaa accatacata
    20401 actgagatgt tggaaaacct tgaatttaag attgggccgc tctctttttt tcaaactaat
    20461 accgaacagg ctctcgtact ctataaaatt gtacggcagt tcggatcatt taaaggtaat
    20521 gaattagttt atgatcttta ttgcggaaca ggaacaataa cctgtttcgt tgccaaaggc
    20581 gtgaagagta ttgttggaat agataatgtg cctgaagcta ttgatgatgc aagagaaaac
    20641 tcgattctaa atggcatttt caataccgct tttgttgtcg gagaccttgc tactaccctt
    20701 aattctgact tcgtcataaa gtatggcgcc cctgatgtta tcattaccga tccgccacgt
    20761 tcaggaatgc accctaaggt tattaagcaa attttggaaa tattacctga aaagataata
    20821 tatgtcagct gcaatccagc cacgcaggcc agagatattt cgttacttgc cgaacaatat
    20881 ctgcttgctg aaattcagcc tgtggatatg ttcccacaga cacatcacgt tgaaaatgtc
    20941 agtcttttaa taaagaaatc cctattttcg gagcaattag tttagccaaa gcattgaaat
    21001 tcaaatcctc tcctttgatt tgaagatgtg ccatctgata aaacgaatct ctttgttcca
    21061 ggtctttttc aataaagcgt ttcagctccg catctttaag gttgatgata cgcgggcgtg
    21121 gccgttttga atttcgaaga cgatgaaaaa gagaatcagg atgcatacgt atgtaaaccg
    21181 atatcccgtg ctcgtttatt tgtcggatgt tatcaccatt gcatacggtg ccgccaccgg
    21241 ttgaaataac atgacttttc aacgaaaagg tgttctccaa catcttccgc tcaatcaatc
    21301 taaaagtatc ttcatcatat ttatcaaaaa aatctacgat gctgatttta taggtctcct
    21361 caaatgccct gtcaagatct aaaaaatcat atcctaataa tcttgcgagc tgcctacccg
    21421 ctgtggattt gccagcgccc atatagccaa tcaagtacac attcttcatc tgaccttttt
    21481 agggcaaaaa tactttttcc tttaaaaagg tggtttttgt ttggaataac aatttgattt
    21541 tcgtaatcaa tatttaactt ataatgattt gaatttgaat gagaaatgcg cttttaaacc
    21601 ataaagaaat tggttggtta aatatctgcc ttatccaatt tcaatactgt tgttgaccaa
    21661 aagttgattt aattctttgg gcgcttttcg atgagatgct tagtaaaacc gactttcatt
    21721 aaaagcaaga aattttgtgg attttcattt caaaaaaaaa catatcaaca atttggtttt
    21781 gtcaaggtta attatctatc tttggacaaa ttgttaggaa ttttttaaac aaaatattat
    21841 ggtattacca aaagatgtta aaccagttga agttttttct ggaactgcct ggcaagctga
    21901 gattatccgt gatatgttgt tggataatga aatagaatct tatcttggcg atgaaatctg
    21961 ggggatggat gctccaatga agagcgcgcc tgacgttgtg ggtagtatta gcgtatttgt
    22021 tgatcgggag gaccttcagg atgctaaaat agtggttgaa agatattatg aagaggattt
    22081 tatttcggag gaggagctgt agcaaccata aaaaacgtaa taaactgatt ataagtcctt
    22141 tcctttacat aaaaagcccg aaaacgattt attttcaggc ttaaaggtga aaaatgtggg
    22201 cattactgga ctcgaaccag tgacttccac cctgtcaagg tgacactctg aaccaactga
    22261 gttaaatgcc caataataaa atagaaactt gatgcaaaaa tagatttttt ttcaagccaa
    22321 aatacttttg cccctatttc aaaaacagaa tgaaccgaaa tgcttattaa agcgagtgaa
    22381 aatttgtagt gtgtaaccaa caacctctcc taactgaccg tttattttcc ttttccctta
    22441 caaaccggat aacagtctct ttgttcttca agttaaataa ttttgaaaag catattgtaa
    22501 ctgcgaaatt cacaaaaaat ggctttcagc cattattggt attacaaaag ccattgcgga
    22561 aatcggcaat tgtggtcttg ttgttttaaa tgcttttaaa atcctgatct tgtaaaaaaa
    22621 tactcattta tgaaagttcg ccagccgtta ccttcaattc caacaatttt cctttcctga
    22681 ttatttttag atttaactcc ttattgatgc atttttcagt aagaagcttg tgcagatcat
    22741 caattgagcc tactacttgg tcattcagtt gaattaggat atcgcctttt tgcaaggcgc
    22801 tgttctctac cccgcttttg ttatcaacgc cgccaatgta cacggctgtt ttcttcacaa
    22861 gtccccattg gtttataagg tcgtttggca gtttgacgtt ttgtccaata ataccaaggt
    22921 atgccctttt tatttttcct tttgtaagca gttcggtagc tatgtattta gcgatatttg
    22981 ccgataccga aaaacaaata ccttgcgctc cttttataat agcagtgtta acacctatca
    23041 cgtgtccttt tgaattaagc aatgggccgc cagagctgcc cggattaatt gctgcatcag
    23101 tctgtatgac atcatcaatt agcctgcccg actgcgttct gagagttcta cccaaagcgc
    23161 ttacaatacc tgaagtaaca gtatgctgaa atcctaatgg atttccgatg gctattgcta
    23221 tctggccagg ctcaagtcga tccgaatcgg tgaaagtaag tggatttaag ccggatgcct
    23281 caatttttag aagcgcaata tctgtggcag catcaacgcc aatgatctta gccggatact
    23341 cttttccgtc aggcgttgtt acgataatct ctccaatatt atttactaca tgatgattgg
    23401 taagtatgat gccattagaa gaaattacaa accctgaacc catagcaaaa gttggataga
    23461 tattttgtac aggacgaccg gcgggcggtt ttcttgtttt tatgttaact attgatttcg
    23521 aagtagcttt caccacacct gttacagtcc gtgagtactc gtcaagataa tttttgtctt
    23581 gtaattcatc agtgtcggca gtatgaagca gaaattcatt attcataatg tggaatttcc
    23641 ctatccttgc caaacatcag gccaaatatc ctatgacgac aaagtggctt aggaatgaaa
    23701 tattgattgt aattttaaat tgataggagt aacacaactt gagagttgat attttttata
    23761 atatataact atctgaaagt caatatgata gtttgaaagg gggagaggtt ttgttgactt
    23821 ttaatgataa aatattttgt tatttcaaat aatgagtact tttgttgctc aatttctata
    23881 tcattttgtt acaaacactg attacatcta agacacgtct tcgcttactg cttaagtttt
    23941 ttttaaacag taacaatatc tcttggttgc gcgaccttga gtcggaattt ggcgaatcta
    24001 ccaatgcaat ccgtgttgag ctgaatagtt tggagaaggc aggtatgctg aaatcaagga
    24061 tggttgggaa caaaaggata taccgtgcca actctgcgca tcctcttttt gatgatattc
    24121 ataatttgtt gttaaaacac accgggattg atcaggtggt tgaacgtatt gtgaataaga
    24181 taggtgggat gaccgaagca tggttagtcg gatcatttgc taaaggaatg gatagccctg
    24241 ttattgatat tttgatgatt ggagatgata tagaccaaac gtatctgacc ggattgatag
    24301 aaaaggttga ggagataata aatcgtagga tcaggtacct gattgtcagt tgtgatgaaa
    24361 aagatataca cctgacacaa tgtcctgaag cttttcagct ttgggaaaaa atagattaag
    24421 ggaaaaggat ttgatagcct gaaaccctta ttaattaagg acttcgttag ttcaagttgt
    24481 gtaactgacg aggctgtgct gtgttaagac ttgatgtatc aatacgaaaa atgacgatag
    24541 gtttaaagaa aactctgtga aataaaaaga aactacacag agctgcatgg agtgcgcgca
    24601 gaataacacg tagggattaa aaactctgtg caacattctt cctggcctca gaaaatcttt
    24661 ttcttttatc ttaacccctt aataatcaat aaactcaaag atttccacct atgtcgaaaa
    24721 ataatacgtt attaattacc ggtggtacag gctcttttgg aaatgcggta ttgcggcggt
    24781 ttttaaacga cgactttttt aaagagatac gcatattgtc ccgtgatgaa aaaaagcagg
    24841 atgatatgcg acagcattac aaaaacgata aaatcaaata ttatataggc gacacccgta
    24901 acaaaagtgc agttacggcc gccatgcgag gagccgattt tgtctttcag gccgccgcct
    24961 tgaaacaagt tccttcttgc gaattttttc ctatcgaggc tgtaaagact aatgtaatgg
    25021 gcgccgaaaa cgtgttggag agcgctattg aacttggtgt gaaaagcgtt gtaatactaa
    25081 gtacggataa ggcagtatat ccaataaacg caatgggcat gagcaaggct ttgagcgaga
    25141 aactcatgat agctaaaagc agaggtttga atggaacagg gcaaactttt tccggtaccc
    25201 ggtatggcaa tgtgatggct tcaaggggct ctgtcattcc gttgtttatt gaacagatca
    25261 aggctgggaa accactcacc attacagatc cggagatgac ccgctttatg atgaccttgg
    25321 atgaagccgt tgaactagta tggtttgcat ttaaaaatgc tgaaaacggt gatatgtttg
    25381 ttcagaaagc tcctgcagct acaattcatg ttttggcaca agctctattg gaattatata
    25441 aagccgataa cgaaattaag attattggaa ccagacacgg agaaaaattg tttgaaacat
    25501 tagtgaacag ggaagaaatg gcaaaagctg ttgacttggg aaattattac agaattcctg
    25561 cagataaccg cgatctcaat tatgggaaat tttttactga aggacaagca aaagtctcag
    25621 aagttgaaga ttatacctcg cacaatacac aacgacttga tgtagaaggc atgaaaaaac
    25681 ttctccttaa actgccacaa attagaaaag atatattggc tgaggatatc tcgaatattt
    25741 atgaaccata aaaaaccgag ctacaggttt aggaatgttt gttttaaggc gtaaagaact
    25801 aagcttttgt tgaaacggaa aattattaaa ggaaagcatt tactaattct agagaatagt
    25861 taaaagttta gtttcacaga gtgccacaga gtactcacag agtagcacag aggagaaaaa
    25921 atactttgag agactatgtg taataaaaa
//
LOCUS       DAOOAI010000017        25224 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_2577_length_25224_cov_4.805713, whole genome shotgun sequence.
ACCESSION   DAOOAI010000017 DAOOAI010000000
VERSION     DAOOAI010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 25224)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25224)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25224
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2577_length_25224_cov_4.805713"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="PK345_02370"
     CDS             complement(<1..1284)
                     /locus_tag="PK345_02370"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0043138 - 3'-5' DNA helicase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HPX33896.1"
                     /translation="MITSKVADYPLHQMLKQFFGFDSFKGLQEPIIRHLLQGNNTFVI
                     MPTGGGKSMCYQLPALLLKGTAIVVSPLIALMKNQVDTLRNFGTKEGIAHFLNSSLTK
                     QEIIQVKNDMTDGFTKLLYVAPESLTKEENVDFLQSIDVSFYAIDEAHCISEWGHDFR
                     PEYRRLRPIIESIGQDVPVIALTATATPKVQQDILKNLNIQNATIFKSSFDRPNLYYE
                     VRPKTKDVFKDIIKYIKSHSGKSGIVYCLSRRKVEELAEVLQVNGIKALPYHAGLDAA
                     TRRTNQDKFLMEDVDIIVATIAFGMGIDKPDVRYVIHHDMPKSLEGYYQETGRAGRDD
                     GEGNCITFYSYDDIQKLEKFMKGKPVAEQEIAQQLLLETVSYAESSICRRRQLLHYFG
                     EMYKQDNCGACDNCLHPKEKIEGKKYIIIVLEAVLD"
     gene            1599..2570
                     /locus_tag="PK345_02375"
     CDS             1599..2570
                     /locus_tag="PK345_02375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KpsF/GutQ family sugar-phosphate isomerase"
                     /protein_id="HPX33897.1"
                     /translation="MAKITDQEIINIAINTLRSESEAISALTAFVDNSFTQCIKLIYE
                     TKERVVITGIGKSAIIGQKIVASLNSTGTPSIFMHAADAVHGDLGIIKPEDIIICISN
                     SGSTPEIKVLIPFIKLMGNKLIAMVGNIDSYLAQQADYIIRTSVEKEVGKSSLAPTTS
                     STAQLAMGDALVVCLMEYRGFTRDKFAMLHPGGTLGKKLFLRVSDIYPNNEKPAVSVD
                     DDIASAIIEMTSKRLGATCVMQGDNLAGIITDGDLRRMMQSGRSIDKLKASDIMTKNP
                     KTVNPEMLVADALDIMRKNNITQLPVKEKNTYIGMIHIHDILKEGIL"
     gene            2615..5101
                     /locus_tag="PK345_02380"
     CDS             2615..5101
                     /locus_tag="PK345_02380"
                     /inference="COORDINATES: protein motif:HMM:NF039501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5686 family protein"
                     /protein_id="HPX33898.1"
                     /translation="MISVFTRYVFIFLLGVLINQASYGQLTKIMGSVVNKETGEPMPY
                     VNIIFNDRAIGITSDFDGKYSIETKNPGDSLIATFIGFEKEIIIVEKGKFQIINFEMI
                     PHEFELQPVVIVAGENPAEILLKKIIENKPNNNPDKFDAYEYEAYNKIQVDISNVSTK
                     MKEWKIFRSFEFIFDYVDTSSVNGKTYLPVFLSETLSNLYFRKNPRSSLEKIQATKMS
                     GIDNESISKFLGDKFQNTNVYDNYILLFEKNFISPIADFGLNYYRYYLIDSAYHDNKW
                     CYKVMFKPRRKQTLTFTGNYWVHDTTFAIKDIELKIADDANINYINDFFISKTYNLIE
                     DKYWLLVKDYAIGDFNLIENNDKVVGFFGRKTTTYSNYLINQPRPDDFYKSPVSIVID
                     KNDSKRPDDFWLKNRHEELTPDERTIYFMVDTLKTLPRFKTYIDILQMIAMGYYEKGL
                     FEYGPYASLFSYNEIEGVRFRIGGRTSNDFSTKVRFSGHLAYGLKDEKFKYGLSGLYM
                     IDKNPRKALGINYIKDLQQLGTSQNAFREDFFMAFLFRRNPADKLSLLEETKINYEHE
                     WFNGFQNTLNFIVQNISSPTNTAFQFYFRNDTIETFKAQNVLTTTEIRLDTRLAYQEK
                     IMMGDFKRISLGAKYPILELRYSYGIPKLFKADFEYHKLQLGISHWFNIMDLGWSKYI
                     IEAGRFWGKLPYPLLKLHEGNETFFFDEYSNNTMNYFEFVSDKYLSAYYTHHFDGYFF
                     NRVPLFRKLKWREVIWGRAIVGGLDHKNRNYSIFPKGMYTLNKPFFEVGAGVENILKV
                     IRFDAVWRLSYLDHADIQKFGLLLSLQFQF"
     gene            5137..5862
                     /gene="lptB"
                     /locus_tag="PK345_02385"
     CDS             5137..5862
                     /gene="lptB"
                     /locus_tag="PK345_02385"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HPX33899.1"
                     /translation="MLLQTQNIYKKYKQRMVVNDVSVSVEQGEIVGLLGPNGAGKTTT
                     FYMIVGLIKPYEGRVLLDDVDITYEPMYKRAQRGIGYLSQEASVFRHLSVEDNIKSVL
                     EFSKFSKAEQKERLELLIEEFGLKHVRKSRGITLSGGERRRTEIARALAVDPKFILLD
                     EPFAGVDPIAVEDIQNIVSKLKDKNIGILITDHNVHETLSITDRAYLLFEGSILRSGT
                     AEELAADEHVRKVYLGQNFELRR"
     gene            5859..6629
                     /locus_tag="PK345_02390"
     CDS             5859..6629
                     /locus_tag="PK345_02390"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPX33900.1"
                     /translation="MTKLSVIIITFNEERNIGRCLQSVKEIADEIIVVDSFSTDETQN
                     ICEQFGAKFISEKWRGYSAQKNFANSLASNNWILSIDADEAISEELKNSIKKWKSQGA
                     PQFARFNRLTNYCGQWIKHCGWYPDTKLRIFHKNQAQWSGEVHEKLKFNEPQQIAFLK
                     GDLLHFSYYSIDEHVAQTNKFSSLAAQQLFISGRHIPFIKALLNPPTIFLRKYIINGG
                     FLDGYYGYVICRLAALQTFLKYFKAWQMQKERKKENKS"
     gene            6587..7696
                     /locus_tag="PK345_02395"
     CDS             6587..7696
                     /locus_tag="PK345_02395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPX33901.1"
                     /translation="MANAERAKKREQVMICFFNTAKTWGGGEKWHFEIASALSDEGIP
                     VILAAHKNSFLLHKATNKKLNTYSFTISNLSFLNPFRIYQLRRFFIKKEIKTVVLNLP
                     SDMKSAGIAARLAGVNRIIYRRGSAIPIRNTLLNRFLFAKIITEVLANSYETKRTILS
                     NNKNIIDPEKITVIYNGVKINELTHNNNRKSSSGSVVIGNLGRLEKQKAQHELIHLAK
                     KLRNAGYQFKMLIGGDGSQKQVLLNKIQDERLQDHVVLTGYVDDIADFMGQIDIFILP
                     SLWEGFGYVLAEAMMHEKPVVAFNCSSNPELIVDGETGFLADANNAEDLFYKTVRLIE
                     DQELRIKMGKAGRLKVAQDFNFKTNFQKIKNFLIC"
     gene            complement(7722..8456)
                     /locus_tag="PK345_02400"
     CDS             complement(7722..8456)
                     /locus_tag="PK345_02400"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HPX33902.1"
                     /translation="MKKKRKAFGRFLRTIVKALRIFLMTIGVVFLIMVALSFTSIPFK
                     VYYWLGTNQVNPKGTPDYIVVMGAGGMPGPGALMRSYFAAEAALLFPEAKVIITLPAD
                     TLNFINSDAYRMAEAIEMKGISADRIIFETNGTNTYTQANEVLKLLGFKDDVNLLIVT
                     SPAHVYRCILTFKKCGFQHVGGLPAFEAGFSPDLLITEKERKQKVKELGRRVSLRYNM
                     WNYLSLEVVVVREFVALGYYKIKGYI"
     gene            8527..10131
                     /locus_tag="PK345_02405"
     CDS             8527..10131
                     /locus_tag="PK345_02405"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPX33903.1"
                     /translation="MTIVKKIPAWFNHTNIYLFGLGLMLVSLPTSKYLMSIAQFILIF
                     NWLIDPKILYKFRDFFKNKPALVVFSIFAIHVIGLLWTSDLHYAWKDLRIKLPLLALP
                     IIISTSPRLNVKTFYYLMLVFIGANLFGSFYSSYKLFTQEIYQPRSAPFISLIRFALN
                     ISVAFFAGIYLSFQKKYFSKVFRIFLALSSIWLLFFLTLMESVTGFAIIVITSIILLT
                     IFVINLKRIVVKSALLFFIIAIPVFMFLFMKSIYDEITPKKPFYHESLDKFTSRGNPY
                     LHDSTLFGIENGNWVGQYINVEELESEWNKRSNIKLDSVDKYGHGLMYTLIRYMTSKG
                     LRKDADGLNALTPEDIHNIETGIASINHLEKRNIKHRLKTVVWEIVLYRQTGYLTATS
                     VTQRLEFWRAGINIIRENLWFGTGTGDIDNAFKQMYPKMKSQLPPDQWWRTHNQYLTI
                     FATLGIFGFLLFIFAMVWPGIKLNMYKDYFFLVFFVISTLSMLTEDTLDTQAGVTFYA
                     FFTMLFLLGRDTINPLIGQSQLFNNK"
     gene            10136..11095
                     /locus_tag="PK345_02410"
     CDS             10136..11095
                     /locus_tag="PK345_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HPX33904.1"
                     /translation="MKNNELSDNKWFAVVNPNAGNRAIKREWKMIHREMTSKGLNFDY
                     ALTEHPFHTLEIVDDALKKGYRKIIAIGGDGTLNEAANAMLDFKDFHPDTHLLALIPA
                     GTGNDWGRMFNVPKKGVQAVKTVIKGKEFTQDVGVITYFDNGQQKKRYFINIAGMGFD
                     ALVAQKTNKQRAEGKSNAYSYLINLLTSFLQYKPSMIQVEIDGTKYNFNTFSISVGIC
                     RYNGGGIMQLPNAIPDDGLLDITIIKKFGLGSVLSLLRNLYNGQFVKHPKVSTFTAKR
                     VTILSKKPGFMMEADGESLGQAPFEITIIPRSLRLIIGSWNSQ"
     gene            complement(11191..15561)
                     /locus_tag="PK345_02415"
     CDS             complement(11191..15561)
                     /locus_tag="PK345_02415"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33905.1"
                     /translation="MRKSIYLALFLLIMSSPFVAFVQNVSNISYINNRQYIDGLPVFT
                     EEDLAMFKDLPEVEMSERLKGRNLPVFVDNSQQPYMRSIFNQSGLECGQAAGVAYLYT
                     YEVNRLRNLPSNVNDNLYPTHFVFNWSNSGSGSAAAFFDSWNIIKELGTPNVTQYGGS
                     LNSGGAYRWMNGYDLYYQSMSNRLWDFYKIPLNNADELEVLKHWIDNHTKGEETGGLA
                     VFYASYSGVYETLPPGTPEAGKYVLTTLHTYANHALTIVGYHDSIRYDLNGDGKYTNH
                     LDINNDGVVDILDWEIGGVKIANSYSATSWGNQGYAYLLYNALCRPLHMGGVWNRSVY
                     VANAKEHSEPQITYKATITHNSRECVKVMAGVAKGYDAQQPEHIIEYPVLNYQGGDKY
                     MQGGSSTADKTLEFGLDVTALLNHLESGEEATFFFLMNEKDPYNNATGVINSFSLIDY
                     TNTMLEIPCQQTNIPIVENGLTMLKVNAAITFEAPEIIIQSLDPATVNEPYEFQMLGG
                     SGKEPYQWKIRQQYSIENGEESFPYISSSQLNPNNSTSGFATKVIDFDFPFYDKSYNK
                     IYIHTDGYLMFTDEPYPWIFLVDEFVFFKNLKNISPYMAKVLGVSQGGGMWYEGDQDM
                     ATFRWKATEYSTGNVLNFAVSLFPSGSIEFFYGEITAAQYNKWHAGISNGDDLNYHLL
                     DISNTYNVQPNTKIVLEPDYSMSDMKITEDGLFYGTPTQSYEDVDISFSLMDSNGMQC
                     LKTLPFSTDGINKIVIRSTEISAGDDNIIEYGETVFVSVKLQNISDEVVNASLMTISG
                     QDQYVGLIDNTQNLNAFEPGEIVFIEDAFSFEVSPYVPNNHNIIFNTQIDALDESYNS
                     HIYLKAYTPVLAVGNVAVDDGNNGLPEPGETIQLLVNIVNSGGGKAYNLNGAILINDP
                     FITIEQGNWSIDLLEGNQVVTAQFEVAIDQNTPIGHVAYLIINVGADQGFGAVDVFAM
                     TIGFVVEDFETGNFSSFDWEFAGNSPWVITNQSPWEGQYCMKSGNISHSQSSTAKVTM
                     EVMVDGEISFYYKVSSETNYDFLSFAIDGSMKGSWSGNIGWTNATYPVQAGERTFSWH
                     YDKDYSVSNGSDCGWIDYIIFPPSVSDVLVAFAGPDMTICENNIPMMQANVANAIYVE
                     WSTTGDGVFNNPEIVNPYYTPGINDIESGSVQLTITAYDDLGSYFVDDMTLFITRLPF
                     VFAGEDLTVCNPSEVELSGSLQFTFVCLWSTSGDGVFTVNNQPVTTYYPGDGDLASGT
                     VELTLKGFSMVPCAESITHTMNVLLTDLPEVEFSQLPEFCIYSPDYQLTEGSPPGGVY
                     SGPGVIDGWFYPSLAGIGLHVLTYTFIDEFGCENFAEQTAFVDECFGLNSGVNVGIDI
                     FPNPTNGSFKIVFDQNIGGNFRIEIFNIKGQRLFNEDINLRREVSDYFLDIGYKPEGI
                     YLLKITCDTMFYTSKLILKK"
     gene            complement(16021..17841)
                     /locus_tag="PK345_02420"
     CDS             complement(16021..17841)
                     /locus_tag="PK345_02420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX33906.1"
                     /translation="MKRPFLLFKYIGKYWGYAALNIIFNILSVIFSLFSLTMIIPFLQ
                     LLFDQTQLIHEAPAFAFNTDSILAYFNYIISGIILSKGPVEALLFISVIVVVMFFIKN
                     LFRYLAMFYLAPLRNGVVQDVRNQIYRRILILPLAYFSEQRKGDIMSRITNDVSNVEW
                     SIMQSLEMLFREPISIIFFLVALIIISPMLTVFVALLLPITAFLIARIGKSLKRTSTK
                     GQKKLGVILSIVEETISGLRIIKGFNAIEAADEKFIKQNTELTRIMNRLYRKRDIASP
                     LSEFLSTIVLVMVLWFGGQLVLSENSTLDPSVFIFYIAVFSQLIVPVKAFTVAYYNVQ
                     KGVASAERIDEIITAEEVILEKPDALPINKFNDHIEYKNVSFAYQLEPVLNNINLRIE
                     KGKMIALVGASGSGKSTMVDLLPRFYDCTEGEIVIDDISIKDLKIDHLRDLMGIVTQE
                     TILFNDTVFNNIAFGLPVASEEDVITAAKIANAHEFIEKMPEQYQTNIGDRGVKLSGG
                     QRQRISIARAVLKNPPIMILDEATSALDTESERLVQDALLKLMKNRTSVVIAHRLSTI
                     QYADEIIVLDKGEIVERGSHSQLIAQNGIYKRLCDMQSFR"
     gene            18023..18361
                     /gene="rbfA"
                     /locus_tag="PK345_02425"
     CDS             18023..18361
                     /gene="rbfA"
                     /locus_tag="PK345_02425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HPX33907.1"
                     /translation="METKRQLRVSKLLQRELSSIFQRDAKDKYGSGMITVTKVNITKD
                     LSIARVYLSLFATKDKAALLESIVQRTPEIRFTLGRAVKDQLRQIPSLEFYEDDSLDY
                     IENIEQLLLP"
     gene            18374..19195
                     /locus_tag="PK345_02430"
     CDS             18374..19195
                     /locus_tag="PK345_02430"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPX33908.1"
                     /translation="MQYDPIKRVLGNIFNHSTWSRKLFYQLLDLLLLRTWHIKKAIRK
                     WLRTIDGAASILDAGSGFGQYSYFLARKNPSWQILGVDVKEEQIEDCQHFFKKAGLTN
                     ANFLLADLTKFIHQESYDLILSVDVMEHIEEDGKVFSNFYQSLKKNGLLLISTPSDKG
                     GSDVHHTNEKHDQKISFIDEHVRDGYSVEEITTKLKNAGFSKVDVAYSYGWPGKISWK
                     LSMKYPISLLNVSKLFFIILPIYYLAIYWFALLMNLLDINLNHNTGTGLIVKAQR"
     gene            19305..20471
                     /locus_tag="PK345_02435"
     CDS             19305..20471
                     /locus_tag="PK345_02435"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX33909.1"
                     /translation="MSVGGVTVGAMALIIVLSVFNGFETLVISLFNSFNPDLEIKVKK
                     GKTFSMSTFPAEEVIKLAGVKALTEVVEENALLKYRNNQFLATVKGVSDNIVNTSGLD
                     TMLIEGRFTLHEMDQPRIILGAGVAYYLNASLNDLLYPITVYLPRREANIGASIEKAF
                     NSENIFPSAVFSIQQDFDTKYTIVPIEFARELLDYEDEVTSIELSLNKSANVEKVKKE
                     IIQLIGNDYEVRNRFEQQVLLYRIMKSEKWSIFLILSFILVIAAFNVISSLTMLILDK
                     KDDVRTLHNLGATDQLIKRIFMLEGMMISIGGAVLGLILGGFISWLQQEFGLISLGDG
                     SGSFVVDAYPVKLLAKDFLIVFLVVLAIGFLVAWYPVKQISKKYLSPKSDYTKQ"
     gene            20625..24734
                     /locus_tag="PK345_02440"
     CDS             20625..24734
                     /locus_tag="PK345_02440"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPX33910.1"
                     /translation="MMIYNSYRSLLSALLLAIFIPFSSISAQIITISPGTNSLSIIEN
                     DLSALSVSNTISEIHANKVKANDVDFIHLSIEGYGFTTTVGHPQLPLLTKLIEVPFEA
                     GFEVEVVSSKFIDISLTELGFDDYIFPAQPSRVKSDQTELLQFYFNQDIYNTNAFLIN
                     DLVQVEHIGILRGVDIARMIVLPVNYNPVANILRVYDHIELRIRFINGSEERTVEMKQ
                     MMNPFFAGIKKMLLNYQEPVKDNLFQNNTITYIIVSDPMFKVALQPLISWKSRKGFNV
                     VDVYTDNPEVGNTALSIKNYLKGFYENPPEGFQPQSYVLLVGDVTQIPAFNGTTGSHV
                     TDLYYCEYTGDYLPEAFYGRFSANNLSQLQPQIDKTLEYEQYLFPDPTFLDEVVMVAG
                     HDSYHQLTWGNGQVNYGNTYYFNQQNGIFSHTYLQPEPAGENYSISIRQNISNGVAFA
                     NYSAHCSASGWTDPSFTVNHIQSLQNQSKYAFMIGNCCSSVDFQNNSFGEEILRATGK
                     GAIGYVGGSANTYWDEDFWWAVGFKSISANPVFANTSLGAFDRIIHSQPGIAINDWHT
                     TQGEAPMAGNLAVTQSGSNLSHYYWEIYHLMGDPSLSVFYSQPSDIWANYPAIIPFGE
                     TTFNINTLPFTYVAVSINNSLLGASFTDEKGNSEINLQEPSAPGEAEIIITGQQLKPF
                     FGNLLIASPEGPYVLVKKTEINDWTLGNGNGQLDYDESFSLNITLQNHGQKSGENIQL
                     LLQSNDEFITILHDTHTVESIEPNATLSLFDLFEISASPGIPDQHPVKFTLVATNGNE
                     NWNSEISLMANAPNFELAGFTLLEIGGNNNGKFDSGETVQFTVFTKNIGHSGINDIKG
                     TLTTNDPYITVLTKEPQYFGNCNPGEIIHALFQVKADQHTPFGHQALFVINFVTETAS
                     FGSNEFSITIGPIPILVVDLDGNHNSAPSINDAADHLGIVSEYAIELPDSLNHFSSIF
                     LSLGVYNANHILSPNEGQKLADYLNNGGRLYMEGGDTWYYDPVTAVHPLFGINGISDG
                     TGNLDTIIGINGTFTKNLKMDFSGDNYWIDQLQATIGATVILKNNNPDFACGIARIGN
                     GFKTIGTSFEFGGLTSEAQRTELLQKYLDFFDITIPASLSSVAYSISNNICEGDTTQM
                     VINISGGSGNFDIQWSPTTGVSNPKSRETLAYPKTTTIYTVTVTDNLTGANTEHQIPI
                     VVKEKPSTPEIIQIDQILISDATTGNQWYNNDGLIEGATKQTFTPTKTNHYWVVVTNT
                     LGCKSEKSNVIYFQSTFIEEPVLQGTIRFYPNPSEGLVYIDFAAERTGKPLIQVYNAF
                     GQPLTNHIIQKNALQNTILLDLRALSNGVYYIKIFDGEHLFTKKLILSK"
     gene            24812..25192
                     /locus_tag="PK345_02445"
     CDS             24812..25192
                     /locus_tag="PK345_02445"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HPX33911.1"
                     /translation="MRSIFLTIVLLFGFISLMAKDGYQVNYKPVSSTVSQIEFTLDDF
                     SLKPVNYDGTDFTKIQFASSVTTEKAGFAELPYLSATIQLKPDKNVDLMVISSEYVDY
                     QLTAPLVPSRGVIYRNQDPVRSHT"
BASE COUNT         8118 a         5026 c         4563 g         7517 t
ORIGIN      
        1 atcaagtact gcttctaaga caataattat atacttttta ccctctattt tctcttttgg
       61 atgaaggcaa ttgtcgcaag cgccgcagtt atcttgttta tacatctcac caaaatagtg
      121 tagtagttgg cgacggcggc aaatggacga ctcggcgtag gaaaccgttt ctaagagcag
      181 ttgttgagcg atttcctgtt cggcaaccgg tttacctttc ataaattttt caagcttctg
      241 tatgtcgtca tagctataaa aagtaatgca gttgccctct ccatcgtcac ggcctgcgcg
      301 gcctgtctcc tgataatagc cttcaaggct tttaggcata tcgtgatgaa tgacataacg
      361 cacgtctggt ttgtcaatgc ccataccaaa tgcaatcgtt gctacaataa tatctacatc
      421 ttccatcagg aatttatcct ggttagtacg gcgggttgct gcatcaagcc cggcatgata
      481 tggcagagct tttattccat ttacctgaag cacttccgca agctcttcca cttttctacg
      541 gctcaggcaa tatactattc ccgatttgcc ggagtgggat ttaatatatt taatgatgtc
      601 tttaaataca tcttttgtct tgggtcttac ttcgtaataa agatttggcc tatcgaagga
      661 tgatttaaaa atagtcgcat tctggatatt caggttcttt agaatatcct gttgaacttt
      721 aggggttgca gtggctgtga gcgctattac cggcacatcc tgtccgatgg attcgataat
      781 aggtcttaat cggcggtatt caggcctgaa atcgtgcccc cattccgaga tacaatgggc
      841 ttcatcaata gcatagaacg aaacgtcaat actttgcagg aaatcaacat tttcctcttt
      901 ggttaaggat tcgggtgcaa cataaagcaa tttggtaaag ccatcggtca tatcgttttt
      961 aacctgtatt atctcctgtt ttgtgagcga ggagtttaaa aaatgagcaa taccttcttt
     1021 tgtgccaaaa ttcctaagtg tgtccacctg atttttcatt aaagcgatca atggcgaaac
     1081 aacaattgca gtacctttta aaagtaatgc aggcaactga taacacattg acttaccacc
     1141 gccagtaggc ataattacaa aagtattatt gccctgaagc agatgtctga tgattggctc
     1201 ttgtaaaccc ttaaagctgt caaacccaaa aaactgtttg agcatctgat gaagcggata
     1261 atccgccact ttagatgtaa tcatttcctt ttccaacata aacatgaatc caaatattca
     1321 ataaaacgtg tacattaacg tactaaacaa aagtcagtta acgaactatt aaaaacaata
     1381 ttttaaaaac ttgcataagt caattaattc ctaaatacaa atctaatatt tttttattac
     1441 taacgagcaa aaattaatat ttaacaaatt taaacaactt ttcatcaaag caatctgatg
     1501 ctatttgtac aatatgatta gtaaaaatag gcctatttta attatcaagt acttttgcat
     1561 aaaaatgacc tctttttttt tcacaaaaag aattatttat ggctaagata accgatcaag
     1621 agatcatcaa tattgcaata aacactttgc gttcggagtc ggaagcgatt agcgccctga
     1681 cagcatttgt ggataatagc tttacacagt gtataaaact gatttacgaa acaaaagagc
     1741 gtgttgttat tactggcatt ggcaagagcg ctattatagg tcaaaaaatt gtcgcctccc
     1801 ttaactcaac aggaacgcca tcaattttta tgcatgcggc agatgctgta catggcgact
     1861 tgggaattat caagcccgaa gatattatta tctgtatttc caatagtggt agcacacctg
     1921 aaattaaagt gttgatccct tttattaaat tgatggggaa caaattaatt gctatggttg
     1981 gcaacataga ttcttatctt gctcaacagg ccgattacat tatccgtacc tctgttgaaa
     2041 aagaggtagg taaatcaagc ttggctccca ccaccagtag tactgcccaa cttgccatgg
     2101 gagatgcatt agtagtgtgc ctgatggaat accgtggttt tacaagggat aaatttgcca
     2161 tgctgcatcc agggggcact ctcggcaaaa agctcttttt acgtgtttcc gacatttatc
     2221 ctaacaacga aaaaccggct gtttcagtgg atgatgatat cgcttcagcc attatcgaaa
     2281 tgacctcaaa gcgtttggga gcaacttgcg ttatgcaggg cgacaacctt gccggtatca
     2341 taaccgatgg cgaccttcga agaatgatgc aatcaggtcg ctcgatagat aaactgaaag
     2401 catctgacat catgacaaaa aacccaaaaa ccgtcaatcc tgaaatgttg gtagccgatg
     2461 cgcttgatat aatgcgcaaa aacaatatta cccaattgcc ggtaaaagaa aaaaatacct
     2521 atatcggtat gatacatatc cacgacatac taaaagaagg catattataa aataatgata
     2581 ttcagaactt tttgatagtt aaaacaaaaa cttaatgata tccgttttta cgagatatgt
     2641 atttattttt ctgctgggag tactaataaa tcaagcatcc tacggacaac ttaccaaaat
     2701 tatgggaagt gttgtgaaca aggaaaccgg cgagccaatg ccttacgtaa atattatttt
     2761 taatgacaga gcaataggta ttacatcaga ttttgatggt aaatactcca ttgaaaccaa
     2821 aaaccccggc gacagtttaa tagcaacatt catcggcttt gaaaaagaga taataatagt
     2881 cgaaaaagga aaatttcaaa ttatcaattt tgaaatgatt ccgcacgaat ttgaattaca
     2941 acctgtagtg atagtcgccg gcgagaatcc ggcagaaatt cttttgaaga aaattataga
     3001 gaacaaacca aacaataacc ccgacaagtt cgatgcatac gaatacgagg cttacaacaa
     3061 aatacaagtt gacatcagca atgtatccac taagatgaaa gagtggaaaa tattcagatc
     3121 gttcgagttt attttcgatt atgttgacac ctcatcagta aatggcaaaa cataccttcc
     3181 tgttttttta tctgaaacat tgtccaactt atattttcgt aagaaccccc gtagctcgtt
     3241 ggagaagata caggcgacta aaatgtcagg aattgataac gaaagcataa gtaaatttct
     3301 tggtgacaaa ttccaaaaca ctaatgttta cgataattac atattacttt ttgagaaaaa
     3361 ttttattagc ccgatcgctg attttggctt gaactattat cgttattatt tgattgacag
     3421 cgcctatcac gacaataaat ggtgctataa agtgatgttt aaaccaagac gtaagcaaac
     3481 acttaccttt acaggaaact attgggttca cgacaccact ttcgcaatta aagatatcga
     3541 attaaaaatt gccgatgatg ccaatatcaa ttacattaac gactttttta ttagcaaaac
     3601 ctacaattta attgaggata agtattggtt attagtaaaa gactacgcta tcggcgattt
     3661 taatttgatt gaaaataatg ataaagtagt tggatttttt ggcaggaaga cgaccaccta
     3721 cagtaattat ctaattaatc agccacggcc agatgatttt tataaatcgc cggtttcaat
     3781 cgttatagat aaaaatgact caaaaagacc tgatgatttt tggttaaaaa atcgccatga
     3841 ggaacttaca cctgacgaaa ggactatcta ctttatggtt gacaccctaa aaacattgcc
     3901 tcgttttaaa acatatattg acattttgca gatgattgcg atgggctatt atgaaaaagg
     3961 gttatttgaa tatggcccat acgcttcgtt attcagctat aacgaaatcg aaggtgtacg
     4021 cttcagaatt ggaggcagga caagcaacga tttcagcaca aaggttagat ttagcggaca
     4081 tttggcctac ggcttaaagg atgagaagtt taaatacgga ttaagcggat tatacatgat
     4141 tgacaaaaac ccgcgtaaag ccctcggcat taattacata aaagacctcc aacagcttgg
     4201 aaccagccaa aacgccttta gagaggattt ttttatggca tttttgttta gacgcaatcc
     4261 cgccgacaaa ctatcacttt tagaagagac caaaattaat tatgaacatg agtggtttaa
     4321 cggttttcag aatacgttga attttattgt ccaaaatata tcgtcgccca ctaatacagc
     4381 ttttcagttt tatttccgca atgataccat tgaaacattt aaagctcaaa atgtccttac
     4441 cacaacagaa ataaggcttg atacgcgtct ggcctatcag gaaaagataa tgatgggcga
     4501 ttttaagcgt attagccttg gggcaaaata tccgatttta gaactgcggt acagctatgg
     4561 gattccaaaa ctgtttaagg cagatttcga gtaccacaag ttacaactgg gaatttcgca
     4621 ctggttcaat atcatggatt taggatggag taaatatatt attgaagctg gccgattctg
     4681 gggcaagcta ccatacccac tattgaaact ccatgaaggt aatgagacct ttttctttga
     4741 tgaatattca aacaacacta tgaattattt tgagtttgtg agcgataaat atcttagcgc
     4801 ttattatact catcattttg acggttattt tttcaatcgt gtacctcttt ttcgaaagct
     4861 gaaatggcgt gaagttattt ggggcagggc tattgtagga gggcttgatc ataaaaaccg
     4921 aaattattct attttcccta aaggtatgta cactctcaac aagccctttt ttgaagttgg
     4981 cgccggagtt gaaaatattt taaaggtaat ccgatttgat gcagtttggc gtttatcata
     5041 tctcgaccat gccgatattc agaaatttgg gttattgctt agtcttcaat ttcagtttta
     5101 atctaacttt gacacttcat ttgattctta cgcaacatgt tattacaaac acaaaatatc
     5161 tacaaaaaat acaagcagcg catggttgtc aacgacgtct ctgtaagtgt tgagcaggga
     5221 gaaatagtcg gactgttggg gccgaatggc gccggtaaga caactacttt ttatatgata
     5281 gtagggctga taaagccata cgaggggcgt gtgttgttag atgatgtaga tattacctac
     5341 gaaccaatgt acaaacgtgc acagcgcggc attggttatt tatcacagga agcctctgtt
     5401 tttcgccatt taagcgttga agataatatt aagagtgttt tggaattttc caaattcagt
     5461 aaagctgaac agaaagagcg tttagagtta ttgattgagg agtttggatt aaagcatgta
     5521 aggaagagcc gtggaataac tctatcgggt ggcgaacgcc gtagaacgga aattgcaagg
     5581 gcgctggccg ttgatcctaa gtttatcctg cttgacgaac catttgccgg tgttgaccca
     5641 atagcggtag aggatattca aaatatagta agcaaactaa aagataaaaa tataggcatt
     5701 cttattaccg accacaatgt acacgaaact ctttcgatca ccgatcgcgc ttatttgctg
     5761 tttgaaggct cgatacttcg ttcgggaact gccgaagaac ttgctgctga cgaacatgtt
     5821 cgaaaagttt atctagggca aaactttgaa ttgagacgat gacaaaacta tcagttataa
     5881 tcatcacgtt caacgaagaa cgcaacatag gccgttgcct gcaatccgtt aaagagatag
     5941 cggacgaaat tattgtagta gattcgtttt cgactgatga aactcaaaat atatgtgagc
     6001 aatttggagc aaaatttatt tcggaaaaat ggaggggcta ctcagcacaa aaaaactttg
     6061 ctaactcatt agcttcaaat aattggattc tctctattga tgccgatgaa gcaatttcgg
     6121 aggagttgaa aaattcgatt aaaaaatgga aatcacaggg agcaccacaa tttgcacgtt
     6181 ttaatcgtct taccaattat tgcgggcaat ggataaaaca ttgcggctgg taccccgaca
     6241 ccaagctacg catctttcac aagaatcagg ctcaatggag tggagaggta catgaaaagc
     6301 taaaatttaa cgagcctcaa caaatagctt tccttaaagg cgatctgctt cattttagct
     6361 attattcaat agatgagcat gttgcccaaa cgaacaaatt tagcagctta gcagcccaac
     6421 agctttttat ctcaggccga cacatccctt tcattaaggc attacttaat cctccgacaa
     6481 tatttctaag aaaatacatt ataaatggcg gttttttaga tggctattac ggttatgtca
     6541 tttgccgttt ggctgcgctt caaacctttc tgaaatattt caaagcgtgg caaatgcaga
     6601 aagagcgaaa aaaagagaac aagtcatgat ctgctttttt aataccgcta aaacctgggg
     6661 aggaggcgaa aaatggcatt tcgaaattgc ttccgcttta tctgatgagg gaattccggt
     6721 aattttagca gctcataaaa attcattttt attgcataaa gcaactaaca aaaagctaaa
     6781 tacatactcg ttcaccatct ctaacttgag ttttctcaat ccttttcgca tttatcagct
     6841 tcgcagattt ttcatcaaaa aagagataaa aaccgttgtt ctcaacttgc cgtctgatat
     6901 gaaatctgcc ggaattgcag caaggttagc aggtgtaaac agaattattt accgcagagg
     6961 cagcgccatc ccaatacgca acacactgtt aaatcgcttt ctatttgcaa agataattac
     7021 tgaagtactg gctaactctt atgaaacaaa acgcacaatt ttatcaaata acaaaaatat
     7081 aattgaccct gaaaaaatta ccgtgattta taatggagtc aaaatcaacg aactgacgca
     7141 taacaacaat agaaaatcct cttccggatc agttgttatc ggaaatttgg gaagacttga
     7201 aaaacagaag gctcaacatg aactgataca tcttgctaaa aaattacgta atgccggtta
     7261 tcaattcaaa atgttgattg gcggcgatgg ttctcaaaaa caagtcttat tgaataaaat
     7321 tcaggatgaa cgccttcaag accacgtagt acttaccggc tatgttgatg atattgcaga
     7381 ttttatgggt cagattgaca tttttattct cccatcgctt tgggaaggtt ttggttatgt
     7441 gctggccgaa gcaatgatgc atgaaaagcc ggtggtggca ttcaactgta gtagtaatcc
     7501 cgaactgatc gtagatggag aaaccggatt tttagctgac gcaaacaacg cagaggatct
     7561 tttttataaa actgtacgcc taattgaaga tcaagagctt agaataaaaa tggggaaggc
     7621 aggacgatta aaagttgctc aggattttaa tttcaaaacc aactttcaaa aaatcaaaaa
     7681 ttttcttatt tgctaaaagc tcttaaatgg ctaatcacac atcatatata tcctttgatt
     7741 ttataatatc cgagcgccac aaactctctg acaacaacaa cctctaaaga aagataattc
     7801 cacatattat atcttaaact cacccttctt cctaattctt tgactttttg ttttctctct
     7861 ttttcggtaa tcagcagatc aggactaaaa cctgcctcaa atgccggcag ccctcctaca
     7921 tgttgaaaac cacatttttt aaatgtaagg atacaacgat aaacatgcgc gggcgatgtt
     7981 acgataagca aattgacatc atctttaaag ccaagcaatt ttaatacttc attggcctgg
     8041 gtataagtat tcgtgccatt tgtttcgaag ataatcctgt cggcgctaat gcccttcatc
     8101 tcaatagctt ctgccattcg ataggcatcg ctattaataa aatttagcgt atcggctggt
     8161 aacgtaataa tgacttttgc ctcggggaaa agcaatgcag cttctgcagc aaaatagctt
     8221 ctcataaggg cgccagggcc tggcatacca ccggcgccca taaccacaat ataatcaggg
     8281 gttcctttcg ggttaacctg attagttcct aaccaataat aaaccttaaa aggtattgaa
     8341 gtaaaactta acgcaaccat aattaaaaaa actactccta tggtcattag aaagatacgc
     8401 aaggctttaa cgatggttct tagaaatctg ccgaaagctt ttcttttctt tttcatttta
     8461 aataatactt ctttgaaaca aaattagttt ttttgtaata aaagaaaatc aaatgattaa
     8521 tcaaaaatga caattgtaaa aaaaatacct gcatggttta atcatacaaa catatacctc
     8581 tttggacttg gattgatgct cgtttctttg cccacatcca aatatctgat gagtatcgca
     8641 cagtttatct tgattttcaa ctggctgatt gatccaaaaa tcctatataa atttcgtgat
     8701 tttttcaaaa acaaacctgc actggtcgtc ttttccattt ttgctatcca tgtaataggg
     8761 ctgttgtgga cttccgattt gcattatgca tggaaagact tacgcataaa gcttccgctg
     8821 ttggccctac caattatcat ctccacttcc ccacgcctga acgtgaagac cttctattat
     8881 ctaatgcttg ttttcattgg agccaacctt ttcgggtcgt tttacagctc ctacaaactc
     8941 tttacacaag agatttatca gcccaggagc gcccctttca tttctctaat acggtttgca
     9001 ttaaacataa gcgtagcatt ttttgcggga atctatttat cttttcaaaa aaaatatttc
     9061 tctaaagttt tcaggatatt tttagctcta tcttcaatat ggttactgtt ttttcttact
     9121 ttaatggagt cagttacagg ctttgctatt atagtaatta cctctattat attgcttacc
     9181 atctttgtaa ttaatttaaa acgaatcgtg gtaaaatcgg cgcttttgtt tttcatcatt
     9241 gccattccgg ttttcatgtt tttatttatg aagtcaattt atgatgaaat tactcccaaa
     9301 aagccttttt atcatgaaag cctcgataaa ttcaccagtc gtggaaatcc ttatttacat
     9361 gacagcactc tatttggtat tgaaaacgga aactgggtag gccaatatat taatgttgaa
     9421 gagttagaat cagaatggaa taagcgaagt aatattaaac tcgactccgt tgataaatat
     9481 gggcacggat tgatgtacac acttattcgt tatatgacct cgaaagggtt gcgcaaggat
     9541 gccgatggcc ttaacgcact gacgccagaa gatatacaca acatcgaaac cggcattgca
     9601 agcattaatc atttagaaaa gagaaacatt aaacaccggc taaaaactgt agtctgggaa
     9661 attgtactat atcgtcaaac gggctatcta accgccacat cagttacaca acgattggaa
     9721 ttttggcgtg ccggaataaa tattatcagg gaaaatttat ggtttggaac gggaaccggc
     9781 gacattgaca atgcatttaa acagatgtac cccaaaatga agagtcagct tcctcccgat
     9841 cagtggtggc gtacacataa ccaatatctg acaatattcg ccacactcgg aatctttggt
     9901 tttttattgt ttatttttgc aatggtctgg ccaggaataa aattgaacat gtacaaagac
     9961 tatttcttcc ttgttttttt tgttatctct accttgagca tgctcactga agatacgttg
    10021 gatacacaag ccggagttac attttacgcc ttttttacta tgcttttttt attaggccga
    10081 gatacaatta accccttaat tggccaaagt caactgttca acaataagta aatccatgaa
    10141 aaacaatgaa ttgtcagaca acaagtggtt tgccgtagta aatcccaatg ccggaaaccg
    10201 tgccattaag cgcgaatgga agatgataca tcgggaaatg accagcaaag gactgaattt
    10261 tgattatgca cttaccgaac atccttttca tactttagag attgttgatg atgctttgaa
    10321 aaaaggatat cgcaaaatca tagccatcgg cggtgatggt accctaaatg aagctgccaa
    10381 cgctatgttg gattttaaag acttccatcc tgatacgcat ttacttgcat tgatccctgc
    10441 cggtaccggc aacgactggg gacgcatgtt caacgttcct aaaaaaggtg ttcaggcagt
    10501 aaaaacagta ataaagggta aggaatttac gcaagatgtc ggagtgataa cctattttga
    10561 taacggccag caaaaaaagc gttattttat caatattgcc ggaatgggct ttgacgcatt
    10621 agtagcacaa aaaaccaata aacaaagggc agaaggtaaa agcaatgcct actcttatct
    10681 gataaatctt ttaaccagtt ttctccagta taagcccagt atgattcagg tagaaattga
    10741 tggaacaaag tataacttca acaccttcag tatcagtgtc gggatatgca gatacaatgg
    10801 cggaggtata atgcagcttc ccaacgcaat tcccgatgat ggtttgctcg atataacaat
    10861 tattaaaaaa tttggcttag gttcagtgct ttcattgtta cgcaatcttt acaacggaca
    10921 atttgtcaag catccaaaag tatcaacctt tacagcaaag agggttacca tactaagcaa
    10981 aaagcctggt tttatgatgg aagccgatgg tgaaagttta ggtcaggcgc catttgaaat
    11041 aacaatcata ccacgctcac taaggttgat tataggcagt tggaattcgc aataataaag
    11101 gaggagctgt acaaaatata aatcctcata aaaccatctt cttaacgagt caaatccttt
    11161 ggctaaattt aaaaaaccac ccttagtaat tcattttttc agaataagtt tagatgtata
    11221 aaacattgta tcacatgtga tctttagtaa ataaatccct tccggtttat atccgatatc
    11281 cagaaaatag tcggatacct cccgtcgcaa attgatatct tcgttaaata gtcgttgacc
    11341 tttgatatta aaaatctcaa tcctaaaatt cccgccgata ttttgatcaa aaacaatttt
    11401 aaaagatccg tttgtaggat tcggaaaaat atctatacca acattgacac cagaatttaa
    11461 cccgaaacac tcgtcaacaa aggctgtttg ttcagcgaag ttttcgcaac caaactcatc
    11521 aataaaggta taagtgagca catgcaatcc aatgcctgcc aatgaaggat aaaaccaacc
    11581 atctattact cctggtcctg aataaacacc acctgggggg cttccttccg taagctgata
    11641 atcgggcgaa taaatgcaga actcgggcag ttgggaaaac tccacctcag gcaaatccgt
    11701 taacagaaca ttcatggtat gagttatact ttcagcacac ggaaccatcg aaaaaccttt
    11761 aagcgtcaat tctacagtac cagaagccaa atcaccgtct cctgggtaat atgtggttac
    11821 cggttgattg ttaacggtaa aaacaccatc gccggaggtg ctccataaac aaacaaaggt
    11881 aaattgcaat gagccggaca attccacttc agatggatta caaacagtta agtcttctcc
    11941 ggcaaataca aatggcaaac gtgtaatgaa gagtgtcata tcatcaacaa agtagcttcc
    12001 aagatcatca taggctgtta tagtgagttg cactgacccg ctctctatat catttattcc
    12061 tggtgtataa taaggattca cgatttccgg attattaaaa acaccatcac ctgttgtgct
    12121 ccattcgacg tatatggcat tagcaacgtt tgcttgcatc atgggtatat tattttcaca
    12181 aatagtcata tcaggtccgg caaacgcaac taaaacatcg gaaacacttg gagggaaaat
    12241 aatgtaatct atccagccac aatcacttcc gtttgatacc gaatagtctt tatcataatg
    12301 ccacgaaaac gttcgctcgc cagcctgtac gggataggta gcattagtcc atccaatatt
    12361 gcctgaccac gagcctttca tactgccgtc aatggcaaaa gagagaaaat catagttagt
    12421 ctcgcttgat actttataat aaaaactaat ctccccatca accattacct ccatggttac
    12481 ctttgcagta cttgattgac tatggctgat gttgcccgat ttcatacaat attggccttc
    12541 ccatggactt tgattagtga tgacccatgg ggaattgccc gcaaactccc aatcgaaact
    12601 actgaaatta ccggtttcaa aatcctcaac aacaaaacca atagtcattg caaaaacgtc
    12661 aactgcacca aatccctgat cagctccaac attaatgata agatatgcca catgacctat
    12721 tggagtgttt tggtcaatag ccacctcaaa ttgcgctgta acaacctgat tgccttcgag
    12781 taaatctatt gaccaattcc cctgttcaat tgttataaaa ggatcattta ttaaaattgc
    12841 accattaaga ttataagcct tgcccccacc cgagttgaca atattcacca aaagctggat
    12901 cgtctcgcca ggctcgggaa gtccattatt tccgtcatct actgctacat tgccaacagc
    12961 taaaaccggc gtataggcct ttaaatagat atggctattg tacgattcgt ctaaagcatc
    13021 tatttgagta ttaaaaataa tgttgtgatt attgggtaca tagggagaaa cctcaaaaga
    13081 aaatgcgtct tcaataaaga cgatctctcc aggttcaaat gcgttcaaat tctgggtatt
    13141 atcaatcaag ccaacgtact gatcttgccc tgaaatggtc attaacgaag cattaacaac
    13201 ttcgtcgctt atattttgca atttaacaga aacaaagact gtctccccat attctataat
    13261 attatcgtct ccggcggaaa tttcagtact cctaataact atcttattaa ttccatcggt
    13321 ggaaaaaggt aaagttttca aacactgcat accgttagaa tccataagcg agaacgaaat
    13381 atcaacatct tcgtacgatt gtgtcggcgt tccatagaat aatccatctt cggtaatttt
    13441 catatcagac atggaataat ccggctctaa aacaatcttt gtattaggtt gaacattgta
    13501 agtattagat atatcaagca gatggtaatt caaatcatcg ccattagata ttccggcatg
    13561 ccatttatta tattgagcag cagttatctc tccgtagaag aactcaatac tgcctgacgg
    13621 gaatagagag acagcgaaat tcaggacgtt tcctgtggaa tactctgtag ctttccaacg
    13681 aaaagtagcc atatcctgat caccctcgta ccacatccca ccaccctgac ttacaccaag
    13741 cacttttgcc atgtatggtg agatgttttt aagattttta aaaaacacaa attcatctac
    13801 aagaaaaatc catggataag gctcatcagt aaacataaga taaccatcgg tatgaatata
    13861 aattttgttg taacttttat catagaacgg gaagtcgaaa tcaataactt ttgttgcaaa
    13921 tcccgaggtg gaattgtttg gattaagttg actggatgaa atgtaaggaa atgactcttc
    13981 tccattctct atactatatt gttgtcttat tttccattga taaggttctt ttccactacc
    14041 tccgagcatc tgaaactcat aaggttcatt aactgttgcc ggatcaagac tttgaataat
    14101 tatctcaggc gcttcaaaag ttattgcagc atttaccttt aacattgtaa ggccattttc
    14161 aacaatcgga atgtttgttt gctggcaggg tatttccagc atcgtattgg tataatcaat
    14221 caatgaaaag ctgtttatca cacctgtggc gttgttgtaa gggtctttct cattcatcaa
    14281 gaagaaaaaa gtagcctctt ccccactttc taaatgattt agcaaagctg taacatcgag
    14341 tccgaactca agggttttat ctgctgttga cgaaccgccc tgcatatatt tgtctccccc
    14401 ctgatagttt aaaacaggat attcgatgat atgctcaggt tgttgagcat cgtaaccttt
    14461 agcaacgccg gccataactt ttacacactc gcgcgagtta tgcgttattg tagctttgta
    14521 ggttatttgt ggctccgaat gctcttttgc atttgctaca taaaccgatc ggttccaaac
    14581 gcctcccatg tgcaacgggc ggcaaagagc attatataag agataggcat atccctgatt
    14641 accccatgaa gtagctgaat aactattggc aatttttaca ccgccaattt cccaatccaa
    14701 tatatcaacc actccatcat tattaatatc caaatggtta gtatattttc catctccatt
    14761 aaggtcgtac cggatggaat catgatagcc aactatagtc agtgcgtggt tggcataagt
    14821 atgaagtgtt gtaagtacat actttccggc ttcgggagtt cccggaggca gagtttcgta
    14881 aacaccgcta tagctagcat aaaaaactgc cagacctccc gtctcttcac ctttggtatg
    14941 attgtcaatc caatgcttaa gtacctcaag ctcatcggca ttattcaaag ggattttata
    15001 aaagtcccac aatctattac tcattgactg ataatataaa tcatagccat tcatccaacg
    15061 gtatgctcct ccagagttca ggctgccacc atattgagta acattaggag ttcctaactc
    15121 ttttataata ttccaactgt cgaaaaaggc tgcggcgcta ccacttcctg aattactcca
    15181 gttaaaaaca aaatgagtag gatataaatt atcgttcaca tttgatggca gatttctcaa
    15241 tctgttaact tcgtaagtat aaagatatgc tacaccggca gcctgcccac actcaagacc
    15301 agactggtta aaaatagacc gcatataagg ttgctgcgaa ttatccacaa atacaggcag
    15361 atttcgtccc tttagccttt ctgacatctc tacttcaggt aaatctttaa acattgccag
    15421 atcctcttca gtaaatacag gtaatccatc aatgtattgc ctattgttta tgtaactaat
    15481 atttgaaacg ttctgtacaa aagcaacaaa gggagatgac attatcagta agaatagagc
    15541 taagtatata gattttctca ttgcttggag gttttcttgg attaaaaaaa gcatcttttt
    15601 ttttacaaaa gtatgaaata tgaaaaaaga aaaaatattc tatccgaact taatatttta
    15661 caatcaacac gttcaaaatt atagttaaac aaaaatgaaa acgggcttta cccctattaa
    15721 ctcaacagtt aggtaaaaaa agctttcttc ttattaattc agatttaaga aaaaacccat
    15781 attctttaca tttttttgag tttgccgaat gaatgaagat ttagataaaa ttagtacaac
    15841 aaaaaaataa tcagatttca ggtgatcaca atatttcaat agagtcaata ttatctaaat
    15901 ttaatgactg aagtagaact taaaataatt aacagctgta tcagggataa acgaagcccc
    15961 caaaacaaat tgtactgcaa atatgcgccc tcgttattgg cattttatca aaattatctt
    16021 ttaacgaaag ctctgcatat cgcaaagcct tttataaatg ccgttttggg cgataagttg
    16081 cgagtgcgaa cctctttcca ctatctctcc cttatccaga acaatgatct cgtcagcata
    16141 ctgaatagtt gaaagccggt gagcaataac tactgaagtt ctgtttttca ttagtttcag
    16201 caaagcgtct tgaacaagtc tttccgattc ggtgtcaagc gccgaagtag cttcatcgag
    16261 tatcatgatt ggtgggtttt tcaatacagc acgtgctata ctgatgcgtt ggcgttggcc
    16321 gcctgaaagt tttacgcctc tatctccaat atttgtctga tactgctcag gcatcttttc
    16381 tataaactcg tgtgcgttgg caattttagc agcagttatc acatcttctt cactcgcaac
    16441 gggtaaccca aaggctatat tattgaaaac agtatcgtta aacaagatag tctcctgagt
    16501 aacaatcccc atcagatcgc gcaaatgatc aatttttaaa tcttttatag agatatcgtc
    16561 tatgactatc tccccctctg tacaatcata aaatctcggc agcaaatcaa ccattgtaga
    16621 ttttccactt cccgatgctc ccacaagcgc aatcattttt cctttctcga ttctcaggtt
    16681 gatattattt aaaacaggct ccagttgata ggcaaatgag acatttttat attcaatatg
    16741 atcattaaac ttgtttattg gcaaagcgtc aggtttttcc agaataacct cttcggcagt
    16801 aataatctca tcaattcttt cggcagaagc aactcctttc tgaacattat aataagccac
    16861 agtaaaagcc tttaccggca ctatcaactg tgaaaaaacg gctatataaa aaataaaaac
    16921 cgaagggtcg agtgttgaat tttcgcttaa gaccaattgc cctccaaacc ataggaccat
    16981 cactaaaaca atcgtgctga gaaactcgct taatggcgaa gcaatatctc ttttacggta
    17041 caaccgattc ataatccttg tcaactcggt gttttgcttt ataaactttt cgtcggctgc
    17101 ctctatggca ttaaaacctt ttatgatcct taaccctgaa atagtctctt ctacgataga
    17161 taaaataact cctaacttct tttgcccttt cgttgaggta cgtttgagac tttttccaat
    17221 ccgtgcgatt aaaaaagcag tgatcggtaa taataatgcc acaaaaacgg tgagcatcgg
    17281 acttataata attagtgcaa cgagaaaaaa gatgatagag atcggctcgc gaaagagcat
    17341 ttcaagcgat tgcattattg accactccac attgcttaca tcatttgtaa tcctcgacat
    17401 aatatcgccc ttgcgctgct cggaaaaata tgcaagtggc aaaataagga tccggcgata
    17461 tatttgatta cgaacatcct gcacaacacc attcctaaga ggcgctaaat aaaacattgc
    17521 aaggtatcga aacaggtttt ttataaaaaa cattacaaca acaataaccg agatgaaaag
    17581 cagcgcttca acagggcctt tgcttaatat tatcccacta ataatatagt tgaaatatgc
    17641 taaaatggag tcggtattaa aagcaaacgc cggggcttca tgtatcaact gcgtttgatc
    17701 aaaaagcaac tgaagaaaag gaattatcat ggtgagcgaa aacaagctga aaataaccga
    17761 caggatatta aaaatgatat taagagcagc atagccccag tacttcccga tgtatttaaa
    17821 aagcaaaaac ggcctcttca tgtgatagag attttgaagt gcaagtttac aaatattaaa
    17881 cctatcaaag acaaaataat catatacgga aaaaaatatc actaaaaaaa actaaaatta
    17941 atgcttaatt ccggctttta ttcatacaaa ccggtaaagt gaattacaaa caaatgagat
    18001 atttttttaa tacttttgca ctatggaaac aaaacgtcaa ttacgagtat caaaattgtt
    18061 acaacgtgag ctaagctcaa tttttcagcg cgatgccaag gataagtatg gctcggggat
    18121 gattacagta accaaagtaa atattaccaa agacctttct atcgcaaggg tttatctcag
    18181 cctgtttgca acaaaagata aggcagcact tctcgaatcc attgtacaac gaactcctga
    18241 aattcgtttt accctcggac gtgctgtaaa agaccagtta aggcaaatcc cttcactcga
    18301 attttatgaa gacgattcgc tcgactacat cgaaaacatt gagcagcttc ttcttcccta
    18361 attcaggaaa cagatgcaat acgatccaat aaagagggtt ttagggaata ttttcaacca
    18421 ctcaacatgg agccgtaagc tcttttacca actcctcgat cttcttctat tgcgaacatg
    18481 gcatataaaa aaagccatca gaaaatggtt gagaacaatt gatggcgcag cctctattct
    18541 tgacgccgga tcgggattcg gacaatatag ttacttcctt gcccgaaaaa acccgtcatg
    18601 gcagattctg ggtgttgatg ttaaagaaga acaaattgaa gactgccaac acttttttaa
    18661 aaaagcagga ttgactaacg ctaacttcct tttggccgat ctcacaaagt tcattcatca
    18721 ggaaagctac gatttaattt tatcggttga tgtgatggag catattgaag aagatggcaa
    18781 ggtctttagt aatttttatc aatctctcaa aaaaaatggc ctgctactca tctccacacc
    18841 ttctgacaag ggaggctctg atgtgcatca tactaatgag aagcatgatc aaaaaatctc
    18901 tttcattgac gaacacgtaa gggatggata tagcgttgaa gaaataacca ctaaacttaa
    18961 gaatgccggt ttttcaaaag ttgatgtggc atactcctat ggctggccag gaaaaatttc
    19021 atggaaacta tctatgaaat accctatttc attattaaat gtttcgaaac tatttttcat
    19081 cattttaccc atttattatc ttgcaatcta ttggtttgcg ctactgatga atctgctaga
    19141 tatcaatctc aaccacaata ccggcactgg tttaattgtt aaagctcaga gataataatc
    19201 taacttaaaa aacattcttt tttatcaaaa cttttccctt ttatattgcc cgtcgttatt
    19261 tatttgctaa gaaatcgcat aatataataa atgtaatctc tgcaatatcg gtaggcgggg
    19321 ttacagtggg cgctatggct ttgataattg tactttcggt atttaatggt tttgaaacac
    19381 ttgttatctc gttgttcaac tcgttcaatc ctgatttaga aatcaaagta aaaaaaggca
    19441 aaactttttc aatgtcaaca tttccggctg aggaagtaat taagcttgcc ggcgtaaaag
    19501 ctttaactga ggtagtggaa gaaaatgcct tgctcaaata ccgaaacaat caatttcttg
    19561 cgactgttaa aggagtgagc gataatattg taaacaccag tgggttagat acgatgctca
    19621 tcgaaggaag gtttacttta cacgagatgg atcaacccag gataatcctc ggagccggcg
    19681 ttgcctatta cctcaatgca agtctcaacg acctgcttta tccaataacc gtttatctgc
    19741 cacgacgcga agccaatatt ggcgcctcga tagaaaaagc attcaacagt gaaaatatat
    19801 tcccctccgc cgtattctcc atacaacagg attttgatac aaaatatacc attgtgccta
    19861 ttgagtttgc aagagagctt ttggattatg aagacgaggt tacttcaatt gaattatcac
    19921 tgaataagag tgcaaatgtt gaaaaagtaa aaaaggaaat aattcagctt atcggtaatg
    19981 attatgaagt acgaaaccgg tttgagcaac aagtgttgct ttaccgaatc atgaagtctg
    20041 aaaaatggtc tatctttttg atcctcagct ttatacttgt catcgccgct ttcaatgtaa
    20101 taagttcgct tactatgctc attcttgata aaaaagatga cgtccgaact ttacataacc
    20161 tcggcgctac cgaccaactc ataaaacgaa tatttatgct cgaagggatg atgatttcta
    20221 tcggtggcgc tgtacttggg ctgattttag gagggtttat cagttggcta cagcaagaat
    20281 tcgggcttat ttcattggga gacggctcag gctcttttgt agtggatgct tatccggtga
    20341 aactgctggc taaagacttc ttaattgtct tccttgtagt gttggcaata ggattccttg
    20401 tagcatggta tccggtaaaa caaatatcaa aaaaatatct ctcaccaaaa agcgattata
    20461 ccaaacaata aatatatcaa gcaaccttat cagactcatc acccttttaa agcaattgtg
    20521 ttttaatttt gtgattaatt ttttgaattc atattttata ttttttttac ctttgccttt
    20581 ttcattaaag actcaaaacc agagctactc aatttgtgag gagaatgatg atttataact
    20641 catatcgcag ccttctttcc gcattgctac tggctatatt catacccttt tcatctattt
    20701 ccgctcaaat cataactatt tcacctggca caaattcatt atcaatcatc gaaaatgatt
    20761 tatcagctct ttcagtatcc aacaccattt ccgaaataca cgcaaataaa gtcaaggcta
    20821 atgacgtaga ttttattcat cttagtattg aaggttatgg ctttacaacc actgtagggc
    20881 atccacaact gccattgtta accaaactta ttgaggtacc ttttgaagca ggttttgaag
    20941 tagaggtggt aagtagtaag tttattgata tctcattgac tgaattgggc tttgatgatt
    21001 atatttttcc ggctcagccc tcaagggtaa aaagtgacca gaccgagttg ctgcagtttt
    21061 actttaatca agatatttac aataccaatg cctttttaat taacgatctc gtacaggtag
    21121 agcatattgg tattttaaga ggtgttgata ttgccagaat gattgtcctt ccggtaaatt
    21181 ataatccggt tgccaatatt ctccgagttt atgaccatat cgaactaaga atcagattta
    21241 taaacggaag tgaggagcgc actgttgaga tgaaacaaat gatgaatccc tttttcgccg
    21301 gtatcaaaaa gatgctttta aattatcagg agcctgtaaa ggacaatttg tttcaaaata
    21361 atactataac atatatcatt gtttccgatc ctatgttcaa ggtagctttg cagcctctta
    21421 tttcatggaa atcaagaaaa ggattcaatg ttgttgatgt atatactgac aatcccgaag
    21481 ttggcaacac tgctttaagt ataaaaaact atttaaaggg tttttatgaa aatccaccgg
    21541 agggtttcca accgcaaagt tatgtactct tggtaggcga tgtaacgcag attccggcgt
    21601 ttaacggaac taccggaagc cacgttaccg atctttatta ctgcgaatat accggtgatt
    21661 atttacctga ggcattttac gggcgattct cggccaataa tctgagtcag ttacagccgc
    21721 aaattgacaa aacccttgaa tacgaacagt atctcttccc cgaccccact ttccttgacg
    21781 aagttgttat ggttgccggc cacgattcct atcatcaact tacttggggc aacggacaag
    21841 tgaactacgg aaacacatac tattttaatc aacaaaacgg tatcttctca cacacatact
    21901 tgcaaccaga gcctgccggc gaaaattatt caatttctat ccgtcagaat atctccaacg
    21961 gagtagcttt tgctaactac tcagcacatt gcagcgcctc aggatggact gatccttcat
    22021 ttacggtaaa tcatatacaa tcacttcaaa atcaaagcaa gtatgctttt atgataggaa
    22081 attgttgctc atccgttgat ttccagaata actcatttgg agaagagata cttagagcaa
    22141 cagggaaagg agctattggc tatgtaggcg gctctgctaa cacctattgg gatgaagact
    22201 tctggtgggc ggtgggtttt aaatctattt ctgcaaatcc ggtttttgct aatacctcct
    22261 taggagcttt cgacagaata atccattccc agcccggaat tgccattaat gattggcata
    22321 cgacacaggg cgaagcgcca atggcaggca atctggccgt tacacagtcg ggatcaaacc
    22381 tttcgcacta ttactgggag atttaccacc ttatgggtga tccttcactg tcggtctttt
    22441 attcacaacc gtcagatatt tgggctaact atccggctat tatcccattt ggcgaaacta
    22501 cgttcaacat caacactctg ccatttactt atgttgccgt ttcaatcaac aattccctat
    22561 taggcgccag ctttacagat gaaaaaggta actcagaaat aaatctacaa gagccgtctg
    22621 cccccggcga agctgaaata ataattaccg gacagcaact gaagccgttt tttggcaacc
    22681 tgttgattgc ttctcctgaa ggaccttatg tattagttaa aaaaaccgaa attaacgatt
    22741 ggacattagg aaatggcaat gggcaattgg attatgacga atcattttca ttaaatatta
    22801 cacttcaaaa ccatggtcaa aaatcagggg aaaatattca actactatta caaagcaacg
    22861 atgaatttat aactatcctc cacgacacac atacagtcga atctattgaa cccaatgcta
    22921 ctctttcact cttcgatttg tttgaaatat ctgcaagccc cggaatacct gatcaacatc
    22981 ctgtaaaatt tactttagta gccactaacg gaaatgaaaa ctggaacagc gaaatctcat
    23041 taatggccaa cgctcctaat ttcgaattag ccggattcac gcttctggaa attggaggga
    23101 ataataatgg gaagttcgac tcgggagaga ctgtgcaatt cacagttttt acgaaaaata
    23161 taggacattc cggtatcaat gacataaaag gaacgctcac caccaatgat ccttacataa
    23221 cggtattaac caaggagccg caatattttg ggaattgcaa tccgggtgaa ataatccacg
    23281 ctctgtttca agtaaaggct gatcagcata ctccttttgg gcatcaggcg ctttttgtaa
    23341 taaattttgt taccgaaaca gcctctttcg gatcaaatga attttctatt actatcggcc
    23401 cgatccccat tctcgttgtt gacttagatg gaaatcacaa ttcagctcct tcgatcaacg
    23461 atgccgccga tcatttagga attgtttcag aatacgccat tgaattaccc gatagtttaa
    23521 atcatttttc atccattttt ctctctttag gcgtttacaa tgccaatcat attttatcgc
    23581 caaatgaagg gcaaaaactt gcagattatt tgaacaatgg cggccggctt tacatggagg
    23641 gtggcgacac ctggtactac gatcccgtaa cggcagttca tccgctattt ggcatcaatg
    23701 gcatttctga cggaaccggc aatctcgata caattatcgg gataaatggc acatttacca
    23761 aaaacctgaa aatggacttc tcaggcgaca actattggat tgaccaatta caggcaacaa
    23821 ttggagcaac tgtaatccta aaaaataata accctgattt tgcctgcggc atagcccgta
    23881 ttggaaatgg atttaagact atcggaacct cttttgagtt tggcggatta acatccgaag
    23941 cacaacgaac tgagttatta caaaaatatc ttgacttctt tgatatcacc atccctgcct
    24001 cgctctcaag tgttgcatac tctatatcaa ataatatatg tgaaggcgac actacccaaa
    24061 tggtaattaa tatttcgggg ggatcgggaa attttgatat ccaatggagc ccgacaaccg
    24121 gggtaagtaa tcctaaatcg cgagagacct tagcttaccc gaaaaccact accatttaca
    24181 ctgttacagt aactgataat ctgacaggtg caaacaccga acatcaaata ccaatagtgg
    24241 ttaaagaaaa accatcaacg cccgaaataa tacagataga ccaaatatta atatcggatg
    24301 ctacaaccgg aaatcaatgg tataataacg atggactgat tgagggcgct acaaagcaaa
    24361 ctttcacgcc aacaaaaacg aatcactatt gggttgttgt aaccaacacg ttaggatgca
    24421 aatcagaaaa atcaaatgtc atatattttc aatctacctt tattgaggaa cctgtattac
    24481 aaggtactat aagattttat cctaacccct cagaagggtt ggtttatatt gattttgcag
    24541 ccgaacgaac tggaaaacct ttaattcagg tttacaatgc ctttggacaa ccactgacaa
    24601 accacataat acaaaaaaat gctttacaaa acactatatt attggatttg cgtgcattgt
    24661 caaatggtgt ttactacata aaaatatttg atggagaaca tctttttacc aaaaaattaa
    24721 tcttgagtaa ataattattt tacaaattgt tagataataa acaaatacaa taaacaatta
    24781 aattctattt cattaactaa actatcttaa catgagaagt atttttttaa caatagtgtt
    24841 gctattcggt tttataagtc tgatggctaa agatggctat caagtaaact acaaacccgt
    24901 ttcgtccact gtgtcccaaa ttgaatttac acttgatgat tttagcctca aacctgtaaa
    24961 ttatgatgga accgacttca ccaagattca atttgcaagt agcgttacta ctgaaaaggc
    25021 tgggtttgct gaacttcctt atctcagcgc tactattcag ttaaagcctg acaaaaatgt
    25081 tgatctgatg gtaatatcct ccgagtatgt ggattatcag ctcaccgccc ctctcgttcc
    25141 ctcaagagga gtaatttatc gaaatcagga cccagtaaga tcccatactt aatcgcacct
    25201 gaatcattgg tagatgattt ctat
//
LOCUS       DAOOAI010000018        24984 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_2616_length_24984_cov_5.078182, whole genome shotgun sequence.
ACCESSION   DAOOAI010000018 DAOOAI010000000
VERSION     DAOOAI010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24984
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2616_length_24984_cov_5.078182"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="PK345_02450"
     CDS             <1..183
                     /locus_tag="PK345_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33912.1"
                     /translation="SVDNHSQDVNWVVTDAKGRYVMEGRLADSHQIDLRTQPKGIYFI
                     KFTGDNVLHIEKLVVR"
     gene            complement(559..2928)
                     /locus_tag="PK345_02455"
     CDS             complement(559..2928)
                     /locus_tag="PK345_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HPX33913.1"
                     /translation="MMTPRIQKLRQNSLDAINTISAERAVLITEFYKSPEARQVSIPV
                     CRAMAFDYLLSKKKICINPGELIVGERGPAPKATPTYPEVCLHTLDDLNMLDKRPKVS
                     YKVDSSVFQTYEEIVIPFWKGQTNRERIMNNMHNEWHEAYAAGVFTEFQEQRAPGHTA
                     GGSKIFEKGMSDIINDIETTISSLDFFNDPEAYNKLEELKAMKIAALAIIKYANRYAE
                     ELEKLALAEKNLHRKQELKEIAAICRNVPKHKPQTFHEALQHYWFIHVGVITELNPWD
                     SFNPGRLDQHLYPFFVKETEQGTLSRENTVELLQAFWVKFNNHPAPPKVGVTALESST
                     YTDFALINLGGVKVDGSDAVNELSYIILDVIEEMRLLQPSSMIQVSKKNPDFFIQRAV
                     KITKTGFGQPSMFNTDAIVQQLVRQGKSIEDARNGGASGCVESGAFGTEAYFLTGYFN
                     LAKVLELTLNNGTDPQSGKLIGIKTGNPNNFKTYNDLLVAFSLQLNYFINIKIKGNNI
                     IEQIIANNLPAPFLSLVIEDCVANGKDYNAGGARYNTSYIQGVGLGSITDSLTAIRYH
                     VFDEKNYSLSEIIEALKKNFKGEEELRYRLIHETPKYGNDDDYADQAAQEVFEIFYKA
                     VNGRPNSRGGKIRINMLPTTSHVYFGSKIDALPDGRKAKEPLSEGISPVQGADRNGPT
                     AVLKSASKIDHILTGGTLLNQKFTPSFFNDNESIARLTHLIRGYFRLDGHHVQFNVVS
                     AKTLKEAQKNPEKYRDLIVRVAGYSDYFNDLSTDLQNEIIRRTEHSEAH"
     gene            complement(2934..3176)
                     /locus_tag="PK345_02460"
     CDS             complement(2934..3176)
                     /locus_tag="PK345_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33914.1"
                     /translation="MIPGFNDSLDTLSKIASTIKLAGDGIREVNLLPYHEMAKSKYAR
                     LNRNFADFKKQKTEKQDITDFINLFETEGFKTKIGG"
     gene            complement(3183..3731)
                     /locus_tag="PK345_02465"
     CDS             complement(3183..3731)
                     /locus_tag="PK345_02465"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HPX33915.1"
                     /translation="MKGVIFDIRRFCIHDGPGIRTTVFLKGCPLQCRWCHNPESQKHS
                     VEVIIKKNVLNSHEIVVNEKIGRFMDAHEVIKEVVKDRVFFDQSDGGVTFSGGEPLAQ
                     ADFLKSLLFLAKEHGIHTTVDTCGYAKVSDLKKIIPYTDLFLYDLKHYDEKPHIEYTK
                     RSNKTILRNLRLLTLSKKKIII"
     gene            4218..5687
                     /locus_tag="PK345_02470"
     CDS             4218..5687
                     /locus_tag="PK345_02470"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HPX33916.1"
                     /translation="MKKKFLSNLILLLFLNLLIKPFWIFGIDRTVQNIVGAEEYGFYY
                     MLFNFSFLFNILLDFGITNFNNRNIAQNNHLLNKHFSSIVIIKLLLAVVYTVVTFSVG
                     LIIGFSLKQLELLGFLCFNQFLLSFILYLRSNISGLLMFRTDSFLSVLDRILMIIICG
                     LLIWGNVTSFSFKIEWFVYAQTTAYLLTAIIALILVMFKASFRRLKWNWPFFVMILKQ
                     SFPFAILTLLMTFYNRIDSVMIERLLPAPKGEYEVGIYASAYRLLDAFNMIAYLFSVL
                     LLPIFSNMIKRKENVVQLVKLAFTLLFIVSATVAIGSHFYSFELMELMYHDHVEESSA
                     VFRLLMFGFIAISSTYVFGTLLTANGSLKTLNIVAASGMVLNLLINLMLVPRLLAVGS
                     AYASLFAQYTTAVIQLIVAARLFKFKVDYRYLLTLAIFLAGLVFLNVFSKQIFENWMY
                     NFMVMVFAAILLAMVLKLLNIKGLIEILKTKENTLEGSS"
     gene            5770..8040
                     /locus_tag="PK345_02475"
     CDS             5770..8040
                     /locus_tag="PK345_02475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33917.1"
                     /translation="MVQQKPINESFDSSNLLFFLYKWRKPLLWVIAVAIVGSTIVSLL
                     LTNKYRSTVIMYPAATNAISQALLTQNRSSMQKGILDFGVDEETEQILQILHSNRIRD
                     RIIEEFGLEQHYGINPESKYRLSKLHRQYENNISFRRTEFMAIRISVLDTDPNYAADI
                     ANRISDLLDSTKSQIQHERAKKAFEIVKSQYFKLADEMQQMEDSLTELRKHGVQDYES
                     QAEMLNMQLAKEMAANNTVGVQRLQEKLDILAQYGGAYVNIRDRLEYEREKLSLLKAK
                     YEEAKTDAEEVLPTKFVVNSAFPAERKSYPIRWLIISVTTISAFFFALIIILILENVK
                     QNFPFIASKAKTKRQQGLNTKDENREINDQFDKQGKPLAFTSKNGTEVKNANKISEED
                     LSVISGSIIEQIKKSVLQELSQQNSQNDSTSNFQIQTEKNDNQMDKLFTNTNLLKMLF
                     KWKIHLAVILLISILVAVVLSSPIVITPKFKSYAVIYPANILSYSEESETEQMLQFLQ
                     SRDIRDHLIAKYDLAQRYKIDSSYKYFQSTIIYEYSKNVKISKTPYESVLIEVFDSDP
                     QVACDMVNDIIYFFNEKMLQTHREKFGEVVDFLQQRLDSKRAEIDSILIVHDDLRINC
                     GIIDFPNQSREVVRGFLRTVDGNNAAANINTKEVLELKKNLELYGGEWIFYNDRLYNL
                     VDEYSKIKIDLDEANMDFNKEITYANIVSAPFPADKKAYPVRWIIVTISAIGALLLFV
                     VIVMVLENYESLKKKF"
     gene            8052..9554
                     /locus_tag="PK345_02480"
     CDS             8052..9554
                     /locus_tag="PK345_02480"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPX33918.1"
                     /translation="MTERIKLIWIYGLSLIFIALNIYFILKEVYWFLVIPLVLVVALL
                     YIFSMDKILLLIAFVTPLSIYLVDPEFNLGISIPAEPLMFGVLIIVILKLIGGFGFDQ
                     KIIRHPLSLSVFAYLGWILVSTLTSEIPIVSVKFFVAKLWFVIPFFFVAAVLFKDFRN
                     IKKFIWLYVISLLIVVVYTTIRHMHYGFTEKAGNWVMRPFYNDHTAYGAILALFIPLV
                     VGFVFTKRHSFSVRMTSFVALLFLLLGLFLSFSRAAWISVAVGILVYIIILLKIKFKY
                     IFGFFFILLTLFFSFQHKIVEVLERNRQDSSTDFIEHVQSIYNISSDASNLERINRWQ
                     AGIRMFQDRPWMGWGPGTYQFLYAPFQRSKEKTIISTNLGDMGNAHSEYIGPLAEQGL
                     PGLLTISFVVIIGVYTALKVRKRSNNPEVRMISLVVIISLVTYFTHGFLNNFLDTDKA
                     SVPVWGLFAIILALDIYHCKEVASAEVIPSVSPPSLAPDSEEPSTDPEAV"
     gene            complement(9493..10449)
                     /locus_tag="PK345_02485"
     CDS             complement(9493..10449)
                     /locus_tag="PK345_02485"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: protein motif:HMM:NF002060.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPX33919.1"
                     /translation="MRLKESISLKKIAKQVNGRFIGDAHLLISGINEIHMVSPGDLTF
                     VDHPKYYEKALKSKATAILINNEMEPPAGKGLIIHDDPFAAYVSLVKLYSPFEPSTSQ
                     ISATATIGEGTIIQPNVFIGNNVTIGSNCLIHAGVSIYDHSIIGNDVIIHANSVIGAD
                     AYYFQRRPHGYRKLESCGRVIIGDKVEIGALCSIDRGVSGDTIIDEGTKMDNHCQVGH
                     DTYIGKNCLIGAHAAIAGVTTIEDDVILWGRVSINKDIFIGKGAIILATSAVDKSIEG
                     NGKVYFGTPVEEARKKWRELAALKQLPDLLKALQNQELKKGG"
     gene            complement(10518..11084)
                     /gene="efp"
                     /locus_tag="PK345_02490"
     CDS             complement(10518..11084)
                     /gene="efp"
                     /locus_tag="PK345_02490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HPX33920.1"
                     /translation="MATTADFKNGLVIIHNDDLYSIVEFQHVKPGKGAAFVRTKLRNI
                     KTGKVLPHTFNAGVKIDIQRVERRPYQFLYKDGSGYHFMNLQTFDQTFFDETLINAPQ
                     FLKEGQEVEILFHSETETPLSCELPAYVELQITYTEPGERGNTATSAMKDATVETGAT
                     VRVPLFINTDEIIRVDTRTGDYSERVKS"
     gene            complement(11178..11975)
                     /gene="lpxA"
                     /locus_tag="PK345_02495"
     CDS             complement(11178..11975)
                     /gene="lpxA"
                     /locus_tag="PK345_02495"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HPX33921.1"
                     /translation="MNQPLAYVHPQAKIAKNVVIEPFVNIEKNVEIDEGTWIGSNVTI
                     MDGARIGKNCRIFPGAVISAIPQDLKFAGEDTVVKIGDNTTIREFVTINRGTKANNET
                     VIGNNCLLMAYVHIAHDCVIRDNVILANAATLAGHIVIDDWAIVGGMAAVHQFVQIGA
                     HSFISGGALVRKDVPPFTKAAREPLSYVGVNSIGLRRRGFSNERINAIQEVYRILYIR
                     GYNTSQAVSIIEADFPASADRDEILAFISRSTRGLMKGYGKIDRRIL"
     gene            complement(11982..13370)
                     /locus_tag="PK345_02500"
     CDS             complement(11982..13370)
                     /locus_tag="PK345_02500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase/3-hydroxyacyl-ACP
                     dehydratase"
                     /protein_id="HPX33922.1"
                     /translation="MMKQRTLKKPAIISGSGLHTGEVVTLTFEPAPENHGIVFKRIDL
                     EGEPEIEALTDFVTDTNRGTCLEKKGVKIYTVEHVMAALAGMQIDNCLVKINGPETPI
                     TDGSSKYFTEALINAGYIEQDSEKDYFEINEVITFNDPSTKSEFIAIPYDHFKVTTMI
                     DFETRVLGTQNAVMENIEDFPVEIAPCRTFAFLHELEFLLKNNLIKGGDLSNAIIFVN
                     KAITQQELDRLAKLFNKPSVEVKSEGILNNLDLYFPNEPARHKLLDVVGDLSLVGKPI
                     KGHIISSRPGHKANTKFAKELRKQMLYQKSKEAVPKYNPNQPALFEIRDIMRFLPHRP
                     PFLLVDKILEMSNTHVIGLKNVTMNESFFVGHFPDEPVMPGVLQIEAMAQTGGILVLN
                     TVPDPENYLTFFLKIDEVKFRNKVVPGDTIIFSLNLIAPIRRGLCHMKGVAYVGDKVV
                     MEGVMLAQISKK"
     gene            complement(13532..14566)
                     /gene="lpxD"
                     /locus_tag="PK345_02505"
     CDS             complement(13532..14566)
                     /gene="lpxD"
                     /locus_tag="PK345_02505"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HPX33923.1"
                     /translation="MTFTATDIAKLLNGIIEGDKNAIVNGLSKIEEGTPGTLSFLANP
                     KYTSYIYQSKASIVIVSKSFVAERTITPTLIRVDDPYSAFASLLEMYNQIKLNKSGIS
                     ELAFISPSARIGKEVYIGAFAYIGDNTCVGDKTKIYPHCYVGDNTSIGSRTTLFSGVK
                     VYSDNIIGNDCVIHAGAIIGADGFGFAPQSDDNYKKIAQIGNVIIEDNVEIGANTTID
                     RATLGSTIIRKGVKLDNLIQIAHNVEIGENTVIAAQSGVAGSGKVGRNCMIAAQVGIV
                     GHLSVGNNVKIAGQSGISTSLPDESIVMGSPAFDAGKYKKAYVHFRNLQKLVDKVDEL
                     EKKIKEMESK"
     gene            complement(14597..15847)
                     /locus_tag="PK345_02510"
     CDS             complement(14597..15847)
                     /locus_tag="PK345_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005785125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPX33924.1"
                     /translation="MQETFGRKKKILNDPVYGFININDELIFDIIEHPWFQRLRRIKQ
                     LGLTHLVYPGALHTRFHHSIGAMHLMQKALEILSSKGHMFSKDERLATLIAILLHDIG
                     HGPYSHSLEKKIVEELSHEELTLLFLERLKRQFGEIFDLAIKIFSGDCSKSFLHQLVS
                     SQLDTDRLDFLKRDSYFTGVAEGVINSDRIITMMEVVSNNLVIEAKGIYSIENFIVAR
                     RLMYWQVYLHKTVLSAEQMLLQVIRRAKLLFADDNDLFGTATLKLFMKNRFTKNDFLN
                     NPLLLNSFGTLDDFDVFTAIKEWAKCDDHVLSLLCQSLVKRNLFRTEIKNKPITEEEI
                     EFRKESVISYYDLSPDEVNYFVLSGSAQNSAYDHTHEGINILFKDGQLLDIAEVSDQL
                     TAEVLSKTVKKYFICYPKAPFDTN"
     gene            15982..17535
                     /locus_tag="PK345_02515"
     CDS             15982..17535
                     /locus_tag="PK345_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PglZ domain-containing protein"
                     /protein_id="HPX33925.1"
                     /translation="MEKIKILWADDEIELLKPHIIFLEQKGYHVVTSNNGDEALDILK
                     EEPFDIVFLDENMPGLSGIETLSKIKVLQPNLPVVMITKSEEETIMEDAIGGRISDYL
                     IKPVKPNQILLSLKKNLENKKIVSEKKTFAYQQEFRDIGMEISKDLDHNQWTEIYKKL
                     VRWELELQQSSDEGIIEVLKMQKEEANQIFSRFIANNYQDWLTSSEPDKPVLSHTVIK
                     EKLFPTLDSTTNTFFLLIDNLRYDQWKVLQPIIEQYYRIESEDIYYSILPTTTQYARN
                     ALFAGLLPTEIEKKYPNYWINEDEEGTKNQFEEQLFAEQLKRYGKDIKFSYHKILNLQ
                     AGKKLADTISNLLQNNLNMIVYNFVDMLSHARTEMEIIRELADDEPAYRSLTISWFEH
                     SPLIDIIRFIASKRLNLVITTDHGSIRVQNPVKVIGDRNTNTNLRYKTGKSLNYNKKE
                     VFEVRNPSDIYLPKLNVSSSFIFCKNYDFFAYPNNYNYYVNYYKNTFQHGGISMEEVL
                     VPYIKLKVK"
     gene            17667..18086
                     /gene="tsaE"
                     /locus_tag="PK345_02520"
     CDS             17667..18086
                     /gene="tsaE"
                     /locus_tag="PK345_02520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007573818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HPX33926.1"
                     /translation="MTTKLIVKSEAELFDVAAKLLGKYPESRIFALYGEMGAGKTTFV
                     KNVCRHLDVFDDVVSPTFSIINEYHTLNNQSVYHFDFYRIKKIEEAFDLGYEDYFFSG
                     AFCFLEWPELIESLLPEDAVKVRISTNEDDQIRLFSF"
     gene            18235..19434
                     /locus_tag="PK345_02525"
     CDS             18235..19434
                     /locus_tag="PK345_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013430.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HPX33927.1"
                     /translation="MNATIREKLLVQEEMLEIAKSKAQLIIGLPMESSFQENRIVLIP
                     DAVKTLVKQGHQVLVQRNAGVASHFPDHEYSEAGARVCESVQEILQADVVLKVSPVSE
                     RELILMKGKQFLISALHLKIQNRNYFQGLMQKKISAMAFEFIKDKTNASPVVRSISEI
                     VGSASIHIAAEYLSHPDFGNGQMLGGFTGIAPSVVVIVGAGTVGEYAARMAISMGAAV
                     KVFDNNVYKLRRLKSEVGQGLFTSTLQTAELQKAIRSADVLICAVHAKFHQSPVLITE
                     EMVASMKQGSVIVDVSIDQGGCVETSRITSHDKPVFNVHGVTHYCVTNIASRFPNTAS
                     IALSNFFAPMLIKAGELGGIEKLLLHDLYFRQGLYTYHGILTNKIIGDYFGLPYQDMD
                     LLMAAFH"
     gene            complement(19459..19938)
                     /locus_tag="PK345_02530"
     CDS             complement(19459..19938)
                     /locus_tag="PK345_02530"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014224845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HPX33928.1"
                     /translation="MSQKAFQDYYPDDFNRCFGCGGLNKHGHQLKSFWDGEETVAEFT
                     PKPWHTTIPGYVYGGLIASLIDCHSTGTASAALYRKEGREMGSEPPIRCVTASLHVDY
                     LRPTPLHGTLKLRATVEKTEGNRVTVKVNLFGGGELCAKGEVVAVAVNEKWMKKWIK"
     gene            20202..20477
                     /locus_tag="PK345_02535"
     CDS             20202..20477
                     /locus_tag="PK345_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HPX33929.1"
                     /translation="MNIFVANLSFKLHEDQLKKVFEPYGEVESARIIVDRRTGRSKGF
                     GFVVMPNEDEAETAIRELNDFEVEGRKLVVKVSEERPEPKRFTSRRE"
     gene            complement(20850..21722)
                     /gene="sucD"
                     /locus_tag="PK345_02540"
     CDS             complement(20850..21722)
                     /gene="sucD"
                     /locus_tag="PK345_02540"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HPX33930.1"
                     /translation="MSVLVDKHSKVIVQGFTGNEGAFHSSQMIVYGTNIVGGVTPGKG
                     GQTHLNRPVFNTVADAVKEVGADVSVIFVPPAFAADAIMEAADAGIKLIVCITEGIPT
                     KDMIIVKEYLKDKNSRLIGPNCPGIITPGEAKIGIMPGFIHSPGCVGIISRSGTLTYE
                     AVDQLTKAGLGQTTAIGIGGDPIIGTTIKEALELFMKDPKTKGIVMIGEIGGTMEAEA
                     AHYYKANCTKPVVGFIAGATAPKGRTMGHAGAIIGGKDDTAEAKKQIMRECGIHVVDS
                     PADIGRKMAELLNA"
     gene            22019..23407
                     /gene="mnmE"
                     /locus_tag="PK345_02545"
     CDS             22019..23407
                     /gene="mnmE"
                     /locus_tag="PK345_02545"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007654033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HPX33931.1"
                     /translation="MDTNEMICALATVPGTAAIAVIRISGSGSIEKISSLFKPAKSNF
                     NLSEANSHTIHYGDIIFDDELLDDVLVSVFRNPHSYTGEDSVEISCHGSPYIQQKLLE
                     NLINSGIRLARPGEFTMRAFLNGKMDLAQAEAVADLIASNSKTAHNLAIDQMRGGFSK
                     RIGELRKELIGFASLIELELDFSEENVEFADRQQLLDLIRKIKTEIESLIQSFNFGNV
                     LKAGIPVAIIGKPNVGKSTLLNTLLQEEKAIVSEIPGTTRDSIEDTIIIDGVAFRFID
                     TAGLRPTEDTIETIGIERTLEKIKQARIILFVFDVATCTMDQIKEVIEEHRHLINDPS
                     KRVILIGNKIDQLIEIPKGFKDLLEMETIFVSAKRKENINLISDSLLKSVSREQIEDN
                     AIVSNTRHYEALSNSLDALNSVEEAMLQGISSDLVASDIRQALYHLGTITGQIVNDDI
                     LDHIFKNFCIGK"
     gene            23586..24350
                     /locus_tag="PK345_02550"
     CDS             23586..24350
                     /locus_tag="PK345_02550"
                     /inference="COORDINATES: protein motif:HMM:NF014037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mut7-C RNAse domain-containing protein"
                     /protein_id="HPX33932.1"
                     /translation="MPDRKVSFRFYEELNDFLPKEKRKKTFSYNFRYNQSVKDAIEAL
                     GIPHAEVDLILVNGNSVDFSYILKNGDYVSVYPVFESLDISLVSRLRNAPLRQVKFIL
                     DVHLGKLCKYLRILGFDTYYRNDLEDSEIVSISVNENRIILTRDIGILKNGKVTHGYW
                     VRSQNSRQQLWEVIHRFDLENILQPFYRCISCNGLIHPVEKEKIEHLLRENTKRFFTD
                     FYQCNACGKVYWEGTHYENMQEFVNNIISRKKLLIS"
     gene            24388..>24984
                     /locus_tag="PK345_02555"
     CDS             24388..>24984
                     /locus_tag="PK345_02555"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HPX33933.1"
                     /translation="MHLKRPTLLIDKQKCLANIRLFVVKAVKYNLRFRPHFKTHQSVE
                     VGSWFREFGIDKITVSSVMMAEYFAKNGWNDITIAFPVNIAETDEINILAKQIKLNLL
                     VENVEAVGSLSRTLKNPVGVYVKIDTGYHRSGIEVEKYDAIADLINIIRRAENLNFKG
                     LLAHNGLTYLQSTPDAICKVHDESLTKLKRLKDSLKKAG"
BASE COUNT         7322 a         4604 c         4904 g         8154 t
ORIGIN      
        1 tcggtagata accactctca ggatgtcaat tgggtagtta ccgatgctaa ggggcggtat
       61 gtgatggagg ggcggttagc tgattcccat cagattgatt tacgcacaca accgaaaggt
      121 atttacttca tcaagtttac cggcgacaat gttcttcata tcgagaaact tgttgtcagg
      181 taatcagcga tgtcagaaat caaaaaagcc ctgctggttg gcggggcttt tttatgagtt
      241 ttcaaaagag gtaaatcgag atgaaataaa aagctccgtt tagaaggtta gttatatctt
      301 ctgtacaaag ctttttgcgc tatgcacctt ttttttattt atactttttc tccaataata
      361 atcatctttt tgtttttagc aacacagtcg cccaacgttt attacaaaac catatcgaaa
      421 aagcgaaagg tacgatgcac cccaatatta aaatcaaatg gtttcgattt tttttagaat
      481 tcaacccaaa taaaatattt gagaagtttt aaaatcaata gcatacttat atatgggctt
      541 tctaaaggtc aattagtttc aatgtgcttc actgtgttcg gtccttcgga taatctcgtt
      601 ttgtaggtct gtactcagat cgttaaaata atcgctgtaa cctgcaaccc gtacaatcag
      661 atcacgatac ttttcgggat tcttttgcgc ttctttaaga gtttttgccg ataccacatt
      721 gaattgcaca tgatgaccat ccaatctgaa gtagccgcgg ataagatgag taagtcttgc
      781 aatactttcg ttatcattaa aaaatgaagg ggtaaatttt tgattaagta aagttccgcc
      841 agtaaggata tgatcaattt tggatgctga ttttaaaact gccgttggcc catttctgtc
      901 agccccctga accggcgata tcccctctga tagtggctct tttgcttttc ttccgtcagg
      961 gagtgcgtct attttgcttc caaaatacac atgacttgta gtaggcaaca tgttgatcct
     1021 gatctttccg cctcttgaat tgggacgtcc gtttacagct ttgtaaaata tttcaaaaac
     1081 ctcttgagca gcctgatcgg cataatcatc atcgtttccg tattttggcg tctcgtggat
     1141 taaccggtat cgtagctcct cctctccttt aaaatttttc tttaaagctt cgatgatctc
     1201 agaaagagaa taattctttt cgtcaaaaac atgatatcgt atagcagtta agctgtcggt
     1261 aatacttcca agtcctaccc cttgtatgta gctggtattg tatcgtgccc cgccggcatt
     1321 ataatcttta ccattagcca cacagtcttc gataactaat gacaggaacg gcgccggcag
     1381 gttgttggct attatttgtt cgattatgtt gttccctttt atctttatgt tgataaaata
     1441 gtttaactgt aagctgaatg ccactaagag gtcgttatat gttttaaaat tattgggatt
     1501 gccggttttt attcctatta atttaccgct ttgcgggtcg gtgccattat ttagggttag
     1561 ttctaatact ttagcgaggt tgaaatatcc ggtaaggaaa taagcttcgg tgccaaaagc
     1621 accactttcc acacatccgc tggcgccgcc attgcgtgca tcctctatgc tttttccctg
     1681 ccttactaat tgttgtacta ttgcatcggt gttgaacata gagggctgtc caaaaccggt
     1741 tttagtaatc ttgacagcgc gctggataaa gaaatccgga ttcttcttgc tgacctgtat
     1801 cattgaactc ggttgaagca gtcgcatctc ttcaatgacg tcgagaatga tatagcttag
     1861 ttcatttact gcgtcgcttc catccacctt tactccacca aggtttatta gagcaaagtc
     1921 ggtgtaggtg ctgctttcca aagctgttac tcctactttg ggcggcgcag gatggttgtt
     1981 aaattttacc caaaaggcct gaaggagctc gacagtattc tctcttgata gtgtgccctg
     2041 ttctgtctct ttaacgaaaa acggatatag atgctggtcg agcctgccag ggttaaagct
     2101 atcccaaggg ttaagctcgg tgatcacacc gacatgaata aaccagtagt gctgcagagc
     2161 ttcatggaag gtttggggct tatgtttcgg aacgttacgg cagatagcgg ctatttcttt
     2221 aagctcttgc tttcggtgca aatttttttc tgctaaagct aacttttcga gttcctctgc
     2281 ataacgattt gcatatttta tgatggccaa tgcggcaatc ttcatcgctt taagctcttc
     2341 caacttattg taagcttccg gatcgttaaa aaagtcaagc gagctgatcg tggtttcaat
     2401 atcattaata atgtcggaca ttcctttttc aaatattttg cttcctcctg ctgtgtgtcc
     2461 tggcgctctt tgttcttgaa actcagtaaa tactcctgct gcataagcct cgtgccattc
     2521 gttgtgcata ttattcatga tccgttctcg attagtctgc cctttccaga agggtatcac
     2581 gatctcttca taggtttgaa aaacgctgct atctaccttg tacgatactt ttggcctttt
     2641 gtccaacatg ttgaggtcgt ccaaagtatg taagcaaact tcagggtatg taggtgttgc
     2701 tttaggcgcc ggcccgcgct cgcctactat cagttcgccg ggattgatac atatcttttt
     2761 tttgctcaat aaataatcga atgccattgc acggcatacc ggaatagaga cctgacgggc
     2821 ttcagggctt ttataaaatt cggtaatcag tacggctctt tccgctgaaa tagtatttat
     2881 tgcatcaagg ctgttttgcc tcaacttttg aatacgtgga gtcatcattt tttttatcct
     2941 cctatttttg ttttaaatcc ttctgtttca aataaattaa taaaatcagt aatatcttgc
     3001 ttttcagttt tttgcttttt aaaatccgca aaatttctat ttagacgtgc atattttgat
     3061 tttgccatct catggtaggg gagaaggtta acttctctta taccatcccc tgcaagttta
     3121 atagtgcttg caatttttga taaagtatct aacgagtcat taaatcctgg aatcaccggt
     3181 attcagatga ttatcttttt tttggataat gttaataaac ggagatttcg cagtatagtt
     3241 ttgttcgatc tcttagtgta ttcaatgtgt ggtttttcgt cataatgttt cagatcgtat
     3301 aaaaacagat cggtgtaggg tataattttt ttgagatccg acactttggc atatccacag
     3361 gtgtccacag tagtgtgaat tccatgttct tttgccaaaa aaagcaatga ctttaagaaa
     3421 tcagcttgtg ccagaggctc gccaccactg aaagtaacac ccccatcgct ctgatcgaaa
     3481 aacactctat cttttacaac ctctttaata acctcatggg catccataaa acggccaatt
     3541 ttctcattaa ccacaatttc atgactattg agaacattct tttttataat aacttcaaca
     3601 ctatgttttt gactttcggg attatggcac caacggcact gcaaagggca tcctttcaga
     3661 aaaactgttg tacgtatgcc cggtccgtca tgtatgcaaa aacgtctgat atcgaaaata
     3721 acacctttca tgtgtgatta aaaaagaaaa attaaatttg agtggtaaag agaatgttga
     3781 tccgttgcga aatcagaaaa gccaacattc ataaaggcct ttgccaaaat tgtggtagag
     3841 gttagaataa tatcgaggcc ttttcatttt aataggttaa tttgagagga caaaattaat
     3901 tcttttttga attgaatctg actctttagc atgtaataag aaaatgtaaa gttagtgagt
     3961 cgtgagttgc ttgtttggct tctttgagcg gcaattgttt taataacaga atgcttgaat
     4021 agtcaacaac acgcaacctt gctactatta aaaccgtcta atctgattaa tattgatttg
     4081 aaatttaaaa ttctacaatt tctatttttg tttaaaacat acttgttttc ggcatgagga
     4141 caattgatta gacaaagggt ggtttgtatt ttaaatttaa ggatagcata atttaggcag
     4201 catattcagc aatttgaatg aaaaagaaat ttctatcaaa cttaatccta ctactatttt
     4261 taaacctgct gataaagcct ttctggattt ttggcattga ccgtactgtt caaaatattg
     4321 taggagccga agagtacgga ttctattaca tgctttttaa tttctctttt ctcttcaata
     4381 tcctacttga ttttggaata accaatttca ataaccgaaa cattgctcaa aataatcatt
     4441 tgctaaacaa gcatttttca agtattgtca tcatcaagct cttgttggca gtagtatata
     4501 ccgtagttac atttagcgta ggattaatta tcggatttag tctcaagcaa ttagagttac
     4561 ttggattttt atgctttaat cagtttctgc tctcttttat tctttatcta cggtcaaata
     4621 tttccggttt gttaatgttt cggaccgaca gttttctttc tgtccttgat cgtattctta
     4681 tgatcatcat ctgcggattg cttatatggg gtaatgttac ctcttttagt tttaagatcg
     4741 aatggtttgt ttatgcacaa acaacagctt acttacttac cgctattatt gcactaattt
     4801 tagttatgtt taaggcttca ttccggcgtt tgaaatggaa ttggcctttt tttgtaatga
     4861 tcctcaagca gagtttccca tttgctatcc ttactctcct tatgaccttt tataatagaa
     4921 ttgattcagt aatgatagag aggctgctgc cggcacctaa aggtgaatat gaggtaggga
     4981 tttatgcttc tgcttacagg ttgctcgatg cgttcaacat gattgcctat ctgttttcgg
     5041 tgcttttatt acctattttc tctaatatga taaaacgaaa agagaatgtt gttcaactgg
     5101 taaagcttgc cttcactctg ttgttcatag tatctgccac cgtagcaata ggttcgcatt
     5161 tttacagttt tgaactgatg gagttgatgt atcatgatca tgtagaggaa tcttcagcag
     5221 tatttcgctt gttgatgttc ggttttattg ctatttcatc aacatacgtt tttggaactt
     5281 tgcttactgc aaatggcagt cttaaaacac ttaacattgt agcggccagt gggatggttc
     5341 tgaatttatt gattaatctg atgctcgtac cccgtttgct tgccgtcggt tctgcctatg
     5401 ccagcctttt tgcacaatat acaacggctg ttattcaact gatagtggct gcccgactgt
     5461 ttaaatttaa ggtggattat cgttatctgt taacattggc tatatttctt gctgggcttg
     5521 tgtttcttaa tgttttctca aagcaaattt ttgaaaattg gatgtataat ttcatggtta
     5581 tggtgtttgc agccatatta ttagctatgg ttttgaaatt attaaatatt aaagggctta
     5641 ttgagatact gaaaacaaaa gaaaacactt tggaagggag tagttgattt ctttgtcgga
     5701 atttaaaatt taaataagta aacaatcaaa aagttctatt tttgtttttt caattgacac
     5761 attaaattta tggttcaaca aaaacctatt aatgagagtt ttgactcttc taaccttttg
     5821 ttttttttgt ataaatggcg taagccgctg ctttgggtta tcgcagttgc tattgttgga
     5881 tcaactatcg tctctctgtt acttaccaat aagtaccgat ctactgtaat catgtatccg
     5941 gcagctacaa atgccatttc gcaggcatta ttaacccaaa atcgtagttc tatgcaaaaa
     6001 ggtatcctcg attttggcgt agatgaggag acggagcaga tactccaaat tcttcattct
     6061 aaccgaataa gggataggat tattgaagaa tttggactgg agcagcatta tgggataaac
     6121 cccgagagca aataccggct tagtaaacta catcgccaat acgaaaacaa catctccttc
     6181 agaaggactg agtttatggc aataaggata tcagtgctcg acaccgatcc taattatgcc
     6241 gcagatattg ccaataggat atctgatctg ctcgattcaa ctaagagtca gatacaacat
     6301 gaacgggcaa aaaaagcttt tgagatagtg aaaagccagt atttcaaact ggccgatgag
     6361 atgcaacaaa tggaagactc gcttaccgag cttcgcaaac atggcgttca ggattatgaa
     6421 tcgcaagctg agatgcttaa catgcaatta gccaaagaga tggctgccaa caacactgtc
     6481 ggtgtgcaac gattacagga gaaacttgat atccttgcgc aatatggagg ggcctacgta
     6541 aatatcaggg ataggttaga atatgaaagg gagaaactca gtttgttaaa agctaaatat
     6601 gaagaagcaa aaactgatgc agaagaggtg ttgccaacaa aatttgtggt aaatagcgcc
     6661 tttcctgccg agagaaaatc atatcctata cgctggctca ttatttcagt tacaacaatc
     6721 tcggcattct tttttgcatt gatcattatc ctgatacttg aaaatgtaaa acagaatttt
     6781 ccatttattg caagcaaagc caaaactaag cgtcaacaag gattaaatac aaaagacgaa
     6841 aatagggaaa taaatgatca atttgataag caggggaagc cgctcgcttt tacttcaaaa
     6901 aatgggacag aggtaaaaaa tgctaataag ataagtgaag aggatctatc tgtaatttcg
     6961 ggatcaatta ttgaacagat taaaaaatca gtactacaag aactctcaca acaaaattcg
     7021 caaaacgatt caacaagtaa ctttcaaatc caaacggaaa aaaacgataa tcaaatggac
     7081 aaattattta ctaataccaa tcttttaaag atgttattta aatggaaaat acaccttgcg
     7141 gtaattttac tgatcagtat tttagttgca gttgtcctct catcgccaat agtgataacg
     7201 cccaagttta aatcctatgc tgtgatatat cctgccaata ttctttcata ctctgaggag
     7261 agcgagacag agcagatgtt acagttttta caatcgaggg acatcaggga tcatttaatt
     7321 gcaaaatatg acttggcgca gcgctataaa attgactcat cgtataaata cttccaatcc
     7381 actattattt atgagtatag caagaatgta aaaatatcta aaacgccgta tgagtcggta
     7441 cttattgaag tgtttgattc cgatccgcag gtggcttgtg atatggtgaa tgatattatt
     7501 tattttttta atgaaaagat gcttcagaca catagggaaa aatttggtga agtggtggat
     7561 tttttacagc aacgattaga ttcaaaaaga gcagaaattg attctatatt aattgttcac
     7621 gatgatttac gtatcaattg tggtattatt gacttcccca atcagtcgcg cgaggtggtc
     7681 agaggctttt taagaactgt agatggaaac aatgcggcag ctaacatcaa tactaaggag
     7741 gttctggaat tgaagaagaa ccttgaattg tatggcggag aatggatatt ttataatgat
     7801 agactttata atttagttga cgagtattca aaaatcaaaa ttgacctgga tgaggccaat
     7861 atggatttta ataaagaaat tacctatgct aatattgtgt cggctccttt tccggctgat
     7921 aaaaaggctt accctgtgcg ttggataatt gttacaatat ctgctattgg agccctgcta
     7981 ctctttgtag ttatagttat ggtgcttgaa aattacgaat ccttgaaaaa gaaattttaa
     8041 ttcgatagat tttgaccgaa cggatcaaat taatatggat ttatggttta agcttgatat
     8101 tcatagcttt gaatatctac tttatactaa aggaggttta ttggtttttg gtaatacctt
     8161 tagtattagt ggttgctttg ctttatattt tttcgatgga taagatatta ttgctaatag
     8221 cttttgtaac accattgtct atttatcttg ttgatcccga gtttaacctc ggtatttcta
     8281 ttccggccga accacttatg ttcggtgttt tgattattgt aattcttaaa ctaatcggag
     8341 gctttggttt tgaccaaaag attatccggc atcctttaag cctctccgtt ttcgcatacc
     8401 ttggttggat tttagtgagt actctaacta gcgagatacc tattgtttcc gttaaatttt
     8461 ttgttgccaa gctatggttt gtgattccat ttttctttgt tgcagcagta ttgtttaagg
     8521 attttagaaa tattaaaaag tttatttggc tttatgtaat ctcgctttta attgtagtag
     8581 tttatacaac cataagacac atgcattatg gttttaccga aaaggccggt aactgggtga
     8641 tgagaccttt ttataacgac catactgctt atggcgctat tttggctctt tttatacctc
     8701 ttgttgtagg gtttgtattt actaaacgcc actccttttc tgtgcgaatg acctcatttg
     8761 tcgctctgtt gtttttgcta cttggtttgt ttctttcttt tagccgtgca gcatggatta
     8821 gtgttgccgt aggcattttg gtttatatta tcattttgct aaaaattaag tttaaataca
     8881 tttttggttt tttctttatt ctactaacac ttttctttag ttttcaacat aagattgtgg
     8941 aggtgttaga gcgtaatcgt caggattcct ccactgactt tattgagcat gtgcaatcca
     9001 tttacaatat cagttctgat gcctccaacc tggaacgtat caatcgctgg caggccggta
     9061 tcagaatgtt tcaagatcgt ccatggatgg ggtggggacc aggtacctat cagtttcttt
     9121 atgccccttt tcaacgcagt aaagagaaga cgattatcag tacgaatctc ggagatatgg
     9181 gtaacgctca cagcgaatat attggccctc tggccgaaca ggggttgccg ggattgttaa
     9241 caatctcatt tgtagtgatt attggtgttt atactgcttt aaaagtgagg aaaagatcga
     9301 ataatcctga agtaagaatg ataagtttgg ttgtaattat atcgcttgta acatacttta
     9361 cccatggctt tttaaacaat tttttagata cggataaggc gtcagtacct gtttggggat
     9421 tgtttgcaat tatccttgcc ctcgatattt accattgtaa agaggtagct tcggcggagg
     9481 tgataccctc tgtcagcccc ccttctttag ctcctgattc tgaagagcct tcaacagatc
     9541 cggaagctgt ttgagtgcag ccaactctcg ccactttttg cgtgcctctt caacaggggt
     9601 gccaaaatag accttgccgt ttccttcgat ggatttgtca actgccgaag tggcaagtat
     9661 tattgcccct tttccaataa aaatgtcctt gttgatagaa acccttcccc ataaaattac
     9721 gtcatcctct atcgttgtaa cgccagcaat ggcagcatga gcgccaataa ggcagttttt
     9781 acctatataa gtgtcgtgcc ctacctggca gtgattatcc atttttgtgc cttcgtcaat
     9841 aatagtatcg cccgatactc cacgatcaat cgaacaaagc gcacctattt caactttatc
     9901 acctattatt acacggccgc atgattcaag ctttcgatat ccatgaggcc ttcgctgaaa
     9961 ataataggca tcggcgccta ttacgctgtt agcatgtata attacgtcgt taccaataat
    10021 actgtggtcg tagatagaaa ccccggcatg gatgaggcag ttacttccaa tggttacatt
    10081 attaccaata aacacatttg gttgaataat agttccctcg ccgatagttg cagtcgcact
    10141 tatttgcgat gtagagggtt cgaatgggct gtaaagctta acaagcgaga cgtatgctgc
    10201 aaaaggatcg tcatgaatga ttagtccttt tcctgccgga ggctccatct cattatttat
    10261 aagtattgct gtggctttgc ttttaagcgc tttttcgtaa tacttcggat ggtcaacaaa
    10321 ggtaaggtcg ccgggcgaaa ccatgtggat ttcattaata cccgaaatta ataaatgcgc
    10381 atcgccgata aatctgccgt ttacttgttt agctatcttt tttagcgaga tagattcttt
    10441 tagccgcatt tttcttaatt ttcttggaaa aaggaagtac tacttgctgt attgatagtc
    10501 cttccacatt atatttatca ggattttacc ctttccgaat aatctcctgt acgggtatca
    10561 actcttatta tttcatctgt atttataaaa agaggaacac gaacagtagc tccggtttct
    10621 acggtagcat ctttcatggc gctcgtggca gtattacctc tttcacccgg ttccgtatat
    10681 gttatttgca actctacata ggccggaagt tcacaagaga gtggagtttc ggtttcagaa
    10741 tggaaaagta tttcaacctc ttgcccttct ttcagaaatt gcggagcatt gataagtgtt
    10801 tcgtcaaaaa atgtctgatc aaaggtttgt agattcataa aatgatatcc agagccatct
    10861 ttataaagga attgataggg cctacgttca acgcgttgaa tatcaatctt tacaccggca
    10921 ttaaaggtgt gggggagtac ttttccagtt tttatgttgc gcagttttgt cctaacaaaa
    10981 gcagcgccct tgcctggctt tacatgttga aattcaacaa tggaataaag atcatcgtta
    11041 tgaatgatca ctaatccgtt tttaaaatct gcggttgttg ccataaaata tcttcaattt
    11101 gattatctaa atattttttt gcaaaaatat atcaaaattt ggaacagaaa ggtgttggat
    11161 agttattcct ttccaaatta taaaatccgg cggtcaattt tgccgtatcc cttcatcaat
    11221 cctctggttg atcggctgat aaatgcaagt atttcatcgc ggtcggcaga agcggggaag
    11281 tcggcttcga taatacttac agcctgagag gtattatacc cgcggatgta taaaatcctg
    11341 tacacctctt gaatagcatt tatcctttca ttggaaaatc ctcgtctgcg caacccgatg
    11401 gagtttacac cgacgtagga gagtggctct ctggcagctt tggtgaatgg tggtacatct
    11461 ttgcgtacaa gggctccgcc ggaaataaaa gagtgagccc cgatttgaac aaactggtgc
    11521 acagcagcca ttccgccaac aattgcccaa tcgtcaataa cgatatgtcc ggcaagtgta
    11581 gcagcattgg caaggattac attatctctt atcacacagt cgtgtgcgat atgaacatag
    11641 gccatcaaca ggcaattatt acctataacc gtttcattgt tggcctttgt gccgcggttt
    11701 atggttacaa attcccggat agtggtgttg tctccaatct ttacgactgt atcttcacca
    11761 gcaaatttca aatcctgagg tattgccgag attactgctc caggaaatat cctgcagttt
    11821 tttccaatac gggcgccatc cataatagtt acattgctac caatccatgt gccttcatca
    11881 atttcaacat tcttttcaat gttcacgaaa ggttctatta ccacgttttt ggcaattttt
    11941 gcctgtggat gaacataagc taacggttga ttcattttga gttatttttt acttatttga
    12001 gcaagcatta caccctccat aactacttta tctcctacat aggctactcc tttcatgtgg
    12061 caaagccctc ggcggattgg tgcaattagg tttaaactga aaattatagt atcgcctgga
    12121 acaactttgt tgcgaaattt aacctcatct atttttagaa aaaaagtgag atagttttcc
    12181 gggtcaggca ctgtatttaa aaccaaaata ccgccggtct gtgccatagc ttctatttga
    12241 agcactcccg gcatgacagg ctcgtcagga aaatggccaa caaaaaaact ttcgttcatg
    12301 gtaacatttt ttaaaccaat gacgtgtgta ttactcatct cgagtatctt atcaaccaat
    12361 aagaatggcg gccggtgcgg taggaaacgc atgatgtccc tgatttcaaa aagtgctggc
    12421 tgattaggat tatattttgg aactgcctct tttgatttct ggtacaacat ctgtttgcga
    12481 agctccttgg caaatttagt gtttgcctta tgtccaggcc tgctggagat gatatgccct
    12541 ttgataggtt tgcctactaa gctaaggtct cccacaacat ctaaaagttt gtgacgagcc
    12601 ggttcgtttg gaaagtaaag atcaagattg tttaagatac cctctgattt gacctctaca
    12661 cttggtttgt tgaagagctt tgcaagacga tccaactcct gttgagtgat ggctttattg
    12721 acaaaaatta tggcattgct taggtctcca cctttgatta aattgttttt aagcaaaaat
    12781 tcaagctcgt gcaaaaatgc aaatgtacgg catggggcta tctctaccgg aaagtcttcg
    12841 atgttttcca ttacggcgtt ttgcgtacct aatacccgtg tttcaaagtc aatcatagtg
    12901 gttaccttga aatggtcgta gggaatggca ataaactcgc ttttagttga agggtcatta
    12961 aatgtgatca cctcattaat ttcgaaatag tccttttcgg aatcttgctc aatatagcct
    13021 gcatttatca atgcttcggt aaaatatttg gagcttccgt cggtaattgg tgtttccggc
    13081 ccattgattt ttacaaggca gttatcaatt tgcattcccg caagggcagc cattacatgc
    13141 tctacagtat aaatttttac ccctttcttt tctaaacaag tgcctcggtt agtatcggta
    13201 acgaaatcag ttaatgcttc aatctcaggc tccccttcca ggtcaattct tttgaaaaca
    13261 attccatgat tttcaggagc aggttcaaag gtcagggtaa cgacctcgcc agtgtgtaaa
    13321 cccgatcctg aaattatcgc aggttttttt agcgttcttt gcttcattat tggcaatgaa
    13381 taatttttaa ttggctgcaa aaataaacat atttcatatt gttttcaaaa gtagtaagag
    13441 cattataaag ggatgctatg cccagctgga tgttgaaggc tacaaataaa aaaagcgaaa
    13501 atgtttatat tgataatgat ttgccgctaa cctatttgct ttccatttcc ttgatctttt
    13561 tttccaactc atccacctta tcaacgagtt tttgcagatt cctgaaatgg acgtaggctt
    13621 tcttatattt tccagcgtca aatgcaggtg atcccattac tatagattcg tctggcaaac
    13681 tggtacttat ccccgactgg cctgctattt taacattatt ccccaccgaa agatgtccaa
    13741 cgatacctac ttgtgctgct atcatacaat tacgaccgac cttacctgac ccggcaactc
    13801 ctgattgtgc tgctatcaca gtgttttcgc caatttctac gttatgtgct atctgaataa
    13861 gattatctaa tttgacacct ttacgtatta tggtagagcc taaagtcgct ctatcaatag
    13921 ttgtgttagc gcctatttca acattgtctt cgataataac attacctatt tgagctattt
    13981 ttttgtaatt gtcatcgctt tgaggtgcaa atccaaatcc atcagcgcca atgatagcgc
    14041 cggcatggat cacgcagtcg ttcccgataa tattatccga atatactttt acgcccgaaa
    14101 aaagggtagt tcttgagcca atacttgtat tatcaccaac ataacagtgc ggataaatct
    14161 ttgttttatc tcctacacag gtgttgtcgc cgatatatgc aaaggcgcca atatagacct
    14221 ccttacctat tctggctgat gggcttataa aggccaattc cgaaatgcca gatttgttaa
    14281 gcttaatctg attgtacatc tccaacagcg aggcaaaggc agagtaagga tcatctactc
    14341 tgataagcgt tggcgtaatt gttcgttcgg caacaaaact ttttgacaca atcactatgc
    14401 tggctttcga ttggtaaata taagaggtgt atttgggatt tgccaaaaag gagagtgtgc
    14461 cgggcgtgcc ctcttcaatt ttagaaagcc cgtttactat ggcgttttta tcaccctcta
    14521 ttattccatt aagtagctta gctatgtctg ttgcagtaaa tgtcattgta tttgaaatta
    14581 gcgattagta aaaaagttaa ttggtatcaa acggagcttt tggatagcaa ataaaatatt
    14641 tttttaccgt ttttgataat acctctgcgg taagttggtc tgatacttcg gctatgtcga
    14701 gtaattggcc atctttgaat aaaatattta taccctcatg agtatggtcg taagcgctat
    14761 tctgggcgct tcccgataaa acaaaataat tgacttcatc aggggataga tcataataac
    14821 tgatgacgct ttcttttcta aattcaatct cctcttcggt gataggttta tttttaatct
    14881 ctgttctgaa aagatttctt ttaacaaggc tctggcaaag taacgaaagc acatgatcat
    14941 cgcatttagc ccattccttg atggctgtga atacatcaaa gtcgtcaagt gttccgaaac
    15001 tgtttaaaag gagggggtta tttaaaaagt cattcttagt aaatctattt ttcataaaaa
    15061 gtttaagtgt ggcagttccg aacagatcgt tgtcatcggc aaaaagtaac ttcgctctac
    15121 ggataacctg aagcaacatc tgctcggccg aaagaactgt tttatgaagg taaacctgcc
    15181 aatacatcaa ccttctggca acaatgaagt tctctatgga atatatccct ttcgcttcta
    15241 tcaccagatt gttacttaca acctccatca tggttatgat acggtcggag ttaataactc
    15301 cttcggctac tccggtaaaa taactatcgc gtttaagaaa atccaaccgg tcggtatcaa
    15361 gttggctcga aacaagttga tgaagaaacg atttggaaca atctccggaa aatattttaa
    15421 tagctaaatc gaatatctct ccaaattgtc ttttaagacg ttccaaaaaa agaagcgtca
    15481 actcttcatg tgagagttct tctacaatct ttttttctaa agaatgtgag tatggtccat
    15541 ggcctatatc gtgtaacaag atggcgatca gggtagcaag tcgttcatct tttgaaaaca
    15601 tgtgtccttt ggatgaaagg atttcaagcg ctttctgcat cagatgcata gcgccgatag
    15661 aatgatgaaa ccgtgtatgt aacgctccag ggtacactaa atgcgtcagc ccaagttgtt
    15721 tgatccttcg caaccgctga aaccaagggt gttcaataat atcgaaaatt aattcgtcgt
    15781 taatgttaat aaaaccgtaa acgggatcgt tgaggatttt tttctttctt ccaaatgtct
    15841 cttgcatatt gcgccagata tgtaatttac ggtttgatcc caacaaaaat gttggaaagt
    15901 caaaataata atttgtaaaa gcttgttata aagataataa accgtgcaaa tttaatttaa
    15961 aaactaattc gaacttattt tatggagaaa atcaaaattt tgtgggctga tgatgagatt
    16021 gaattgctaa aaccacacat catttttctt gagcaaaagg gatatcacgt tgttacatcc
    16081 aataacggag atgaagcgtt ggatatactg aaagaagaac cttttgacat tgtgtttctt
    16141 gacgaaaaca tgccaggact gtccggcatc gaaacgcttt ccaaaataaa agtcttacag
    16201 cctaatttgc cggtagtgat gattaccaaa agcgaggagg agacaataat ggaagatgct
    16261 atcggtgggc gaatatctga ttatttgatt aaaccagtca agcctaatca gatattgcta
    16321 agcctgaaaa agaacctcga aaataaaaaa atagttagcg aaaaaaagac ttttgcctat
    16381 caacaggagt ttcgcgatat tggcatggaa atctccaaag atttagatca taatcaatgg
    16441 actgaaatct ataaaaagct tgtacgttgg gagttagagc ttcaacaatc ttctgatgaa
    16501 ggaatcatag aggttttaaa gatgcaaaag gaggaggcca atcagatatt tagccgtttc
    16561 atagccaaca attatcagga ctggcttaca agcagtgaac ctgataagcc tgtactctcg
    16621 catacagtga taaaagagaa gctgttccca acgcttgatt ctacaacgaa tacctttttc
    16681 ttactgattg acaaccttcg ttatgatcag tggaaggtgt tgcaacctat tatcgagcaa
    16741 tattaccgga tcgaaagcga ggatatatat tacagcattc ttcctacaac aacacagtat
    16801 gcacgtaatg cattgtttgc cggactattg ccaaccgaga tagaaaaaaa atatccgaat
    16861 tattggataa atgaagatga agagggtaca aaaaaccagt ttgaagaaca actatttgct
    16921 gaacagctca agcgttatgg taaagatatt aagttttcgt accataagat actcaatctt
    16981 caggccggaa aaaaattagc tgataccata tctaatttat tgcaaaacaa ccttaatatg
    17041 attgtttata attttgtgga tatgctctcg catgcacgaa ccgagatgga gatcattcgc
    17101 gaacttgctg atgatgagcc tgcatatagg tcgctcacta tctcatggtt cgaacattca
    17161 ccattgattg atatcataag gttcattgca tcaaaacgac taaatctcgt tatcacaact
    17221 gatcatgggt caataagggt acagaatccg gttaaggtaa tcggagatag gaacactaac
    17281 accaacctgc gatataaaac tgggaagagt ttaaactaca ataaaaaaga ggtgtttgaa
    17341 gtgcgaaatc cgtcagatat ttacttgcca aagctaaatg taagctcctc atttatcttc
    17401 tgcaaaaatt acgatttctt tgcctacccg aataattaca actactacgt aaactattat
    17461 aaaaacacct ttcagcatgg cgggatatct atggaggagg ttttagttcc atatatcaag
    17521 ctgaaggtca aatagtaatt aaattgtaag cgataaatgc caaagtcctt gggtaaaaag
    17581 atttcttgaa atttaaactc gctgttgcga ctttgttttt ttaaagtgat ttagggatgt
    17641 gcttctaaat tataattctt ttgaatatga cgaccaaatt gattgtaaaa tcggaggctg
    17701 aactttttga cgtagctgct aaattgctgg gaaaatatcc tgaatcccgc atttttgctt
    17761 tatatggtga aatgggagcc ggtaaaacaa cctttgtgaa aaatgtatgc cggcatttag
    17821 atgtttttga tgacgttgtc agccctacct tttccattat aaatgaatat cataccctaa
    17881 ataatcaatc ggtctatcat tttgattttt atcggattaa aaagattgaa gaggcttttg
    17941 atcttggtta tgaggattat ttctttagcg gtgcattttg ctttttggaa tggcctgagc
    18001 ttattgaatc cttgctgccc gaagatgctg taaaagttag gatatccact aatgaagacg
    18061 accaaatcag gctttttagt ttttgaaggt cagcaattga tttttttgtc agagccgtaa
    18121 ttttgatata gttataaaaa ggtttggtct atttttttta ttattaaata aaaagagttt
    18181 acttttgata cgaatttgga aaacaatagg atagaataat aaatgacttt ataaatgaat
    18241 gcaacaatac gggaaaagct tttggtacaa gaggagatgc ttgaaattgc taaatcaaag
    18301 gctcaactga ttataggcct gcctatggaa tcctcatttc aggaaaacag gatcgtattg
    18361 ataccagacg cggtaaaaac gcttgtaaag caaggtcatc aggtgcttgt tcagcgaaat
    18421 gccggtgtag cttcacattt tcctgatcat gaatattcag aagctggagc tagagtttgc
    18481 gaatctgtgc aagagatttt gcaggctgat gttgttttaa aagtctctcc ggtttcggaa
    18541 agagagctta tattgatgaa aggtaagcag tttcttatta gcgccctgca tcttaagatt
    18601 caaaaccgca attattttca gggattgatg cagaagaaaa tatcggctat ggcctttgaa
    18661 ttcataaaag ataaaacaaa tgcttcgcct gtagtcaggt caataagcga aattgtcggt
    18721 tctgcttcta tccatattgc tgctgaatac ctctctcatc ctgattttgg gaatggtcaa
    18781 atgttaggtg gttttaccgg catagcgcca tcggttgttg tgatagttgg agctggaacg
    18841 gtgggcgaat acgctgctcg tatggctatt agtatgggcg ctgcggtcaa ggtttttgat
    18901 aataatgttt acaaactccg aaggctgaag tcggaagtag ggcaagggtt gtttacctca
    18961 acacttcaaa ccgctgagct gcaaaaggca atacgctcgg cagatgtatt gatatgcgct
    19021 gttcatgcaa agtttcacca aagtcctgtt ttgattactg aggagatggt agcctctatg
    19081 aaacaaggat cagtaatcgt tgatgttagc attgatcagg gcggttgtgt cgaaacttca
    19141 aggattacct ctcacgataa gcccgtcttc aatgttcatg gtgttacaca ttattgcgta
    19201 actaatattg cttccagatt tccaaatacc gcatccatag ctctcagtaa tttttttgct
    19261 ccaatgctta ttaaagccgg tgaactgggc ggtattgaaa aactattatt acatgattta
    19321 tactttcggc aaggacttta tacctatcat ggtattttga ctaacaaaat tataggtgac
    19381 tatttcggat tgccttatca ggatatggac ttactgatgg cagcatttca ttaatcaaaa
    19441 cttctgattc cgatacggct atttgatcca tttcttcatc cacttttcgt taactgccac
    19501 tgccactacc tcccctttag cacataattc gcctccacca aacaagttta cttttaccgt
    19561 tactctgtta ccttctgttt tttcgactgt tgccctaagc tttaaagttc cgtgcagagg
    19621 agtaggtctt aaataatcaa catgtaatga tgcagtaacg caacgtattg gtggttcgct
    19681 gcccatttcg cggccctctt tgcgataaag tgcagcagag gcagtgcctg tagagtggca
    19741 gtcaataagc gaggctataa gaccgccgta aacgtatccg ggtatggtgg tgtgccatgg
    19801 ttttggagtg aattcagcca ccgtttcttc accatcccag aaactcttga gttgatgccc
    19861 atgtttattt aaaccaccac aaccgaagca acggttaaag tcgtcagggt aataatcttg
    19921 aaaagctttt tgactcattt tgagtgattt tttaattttt caaaaatcta atttttatat
    19981 taaacaggga ttaaccctat tttttaatat gataatgtat ttaaatccag cgttttaata
    20041 aattattttc agtaatagtt ttttatattt aattttaatt acttttgccg gctaaagcta
    20101 aaaacggaaa aatgaaaaca tcagtgttga aatcctgaga ttcactattt ttgtttgtgc
    20161 ttttagagta aatcaatgtc ttattttttt atttttacat tatgaacatt tttgttgcaa
    20221 acctgagttt caaattacat gaagatcagt taaaaaaagt ttttgaacct tatggcgagg
    20281 ttgaatctgc caggataatt gtggatcgtc gtactggacg ttctaaagga ttcggttttg
    20341 ttgtgatgcc aaacgaagat gaggcagaaa ctgctattag ggaacttaat gattttgagg
    20401 tagaaggtag gaaattggtt gtaaaagttt ccgaagaacg ccccgaacct aaacgtttta
    20461 caagtaggcg tgaataatca ctaacaactc ttattaataa gatttttgag gcaggctgta
    20521 aaagtctgcc tttttttatt ttaactcaca tcatgccttt ttttatttcg gtcaacacat
    20581 tgttagtgat tcaactaatt gtatgaagtc ttgcaaactt ctccaaggcc tatttttcaa
    20641 gttactcttt gtcataaaca acactggctg ccatttttta aatgttctat aacgggatga
    20701 aaattggcta tggtcatgtt ttttagcgct gatcttaatg cgaaacacag aagttgaatt
    20761 ttgatcccta tagttaccgg aattgatacc aagtcgttca gtttgcctat tgctaatttg
    20821 ggaataaatg tagctccaaa taaagacact taggcattta gcagctcagc catttttctg
    20881 cctatatctg ccggtgaatc aacaacatgg atgccgcatt cgcgcattat ttgtttttta
    20941 gcttctgcag tatcgtcttt tccgccaatg atagcgccgg catgtcccat agtacgacct
    21001 tttggtgcag tagctccagc aatgaaaccg actactggtt ttgtgcaatt tgctttataa
    21061 taatgagctg cttcggcctc catcgtgcct ccgatttcac caatcatcac tattcctttt
    21121 gtttttgggt ccttcatgaa gagttcaagc gcctctttaa ttgttgtgcc gattatagga
    21181 tcgcctccaa taccgatagc tgttgtttgt cccaaaccgg ctttagtaag ctgatcaacg
    21241 gcttcgtaag tgagcgttcc ggaacgtgag attatgccaa cgcatccggg cgaatggata
    21301 aaacctggca taatacctat ttttgcttca ccaggggtaa tgataccggg gcagtttgga
    21361 cctattaaac gagaattctt gtctttcaga tattctttta ctatgatcat atctttggtt
    21421 ggaatacctt cggtaataca tacgatcagc tttattccgg catcggcagc ttccatgatg
    21481 gcatcagcag caaaagccgg tggaacgaaa atcacagata cgtcagctcc gacctctttt
    21541 accgcatcag ccacagtatt gaaaacaggc ctgttgagat gtgtctgacc acccttgcca
    21601 ggtgtaaccc ctccaactat attggtgccg taaacaatca tctggctgga atgaaaagct
    21661 ccttcgttac cggtaaagcc ttgtacgata acttttgaat gtttgtcaac taagacgctc
    21721 attgttgtta aattatgaat tgattaaata aaaatttgag aacaaaaata ttccgtttta
    21781 gtaatttgcc ggcaattatt aactattttc gaaaaaatta tttataaaat tatggtttaa
    21841 gcaattttta ttaatgatta cttagtgact ttaaattaaa ataacgcatt gaatatcaat
    21901 gaataaacat tattgttgaa aagtttttag ataaatgtaa aagattatca tcttccaata
    21961 attaatggta ttggtaaaaa gctaattttg cttgtatcaa aaaccgaaac agatagttat
    22021 ggacacaaat gaaatgattt gcgcacttgc taccgttcca ggtactgcgg ccattgctgt
    22081 tattagaatt tccggttctg gctctatcga aaaaataagt tcgttattta agcctgctaa
    22141 aagcaatttt aatctttctg aggctaatag ccataccatc cattatggag atataatttt
    22201 tgacgatgag cttttggatg atgtgttggt tagtgttttc agaaacccac attcctatac
    22261 gggtgaagac tccgttgaga tctcgtgtca tgggtctccc tatattcagc aaaagctttt
    22321 ggaaaacctg atcaattctg gaataagact tgccaggcca ggtgaattta ccatgcgagc
    22381 ctttttaaat ggaaaaatgg atcttgcaca ggctgaagct gttgccgatc tgattgcctc
    22441 taactccaaa actgctcata atctggctat tgaccagatg cgaggagggt tttcaaaaag
    22501 aataggggag ttgcgcaaag agcttattgg gtttgcttcg cttatagaac ttgaattaga
    22561 tttttctgag gagaatgttg aatttgcaga tcgccagcag ctgttagacc tgataagaaa
    22621 gataaaaacc gaaatagaga gccttattca atcttttaac tttggaaatg tattaaaagc
    22681 tggaattccg gttgcaataa ttgggaaacc aaatgtagga aaatctacct tgctcaacac
    22741 cttacttcaa gaagagaaag ctatagtttc ggaaattcca ggcacaacac gcgactcaat
    22801 agaagatacc attattatag atggcgtggc attccgattt attgatactg ccggtcttcg
    22861 ccctaccgaa gataccatcg agacaatcgg tatagaacgt acattagaaa agataaaaca
    22921 ggcacggata attctgtttg tatttgacgt agctacttgc accatggatc agataaaaga
    22981 ggtaatcgag gaacatcgtc atttgataaa cgacccttca aaacgcgtga tactcattgg
    23041 taataagatt gaccaactga tagagattcc aaaaggcttt aaagatttac ttgaaatgga
    23101 gactattttc gtctcggcaa aacgtaagga aaacataaat ctgatttcag atagtctgtt
    23161 gaaatcggtg tcaagagagc aaattgaaga caacgcaatt gtatcaaata cacgccacta
    23221 cgaggcatta tcaaattcgc tcgatgctct taactcggtg gaagaagcaa tgttacaggg
    23281 tatatcatct gatctggtag caagtgacat acggcaagcc ttataccacc ttggaacaat
    23341 taccggccag attgtcaacg acgacatact cgatcatatt tttaaaaatt tttgtatagg
    23401 taaataaatt attctaaaga atagtttata acatctatga aagcaataaa tccttattag
    23461 caatttagaa aatcaagctg atcttacgaa ctacaaaatg ctaatcagag ttgctgagaa
    23521 ttaatatttc ttaatatgct tatcaaggcc ttttccgaac aaattttgaa aatccgataa
    23581 tgacaatgcc ggatagaaaa gttagtttca ggttttatga agagcttaat gattttcttc
    23641 cgaaggagaa acgaaaaaag actttctcgt ataatttccg ctataatcaa agtgtaaaag
    23701 atgctattga agctttggga ataccacatg cagaggttga tttgatcctt gtaaacggaa
    23761 attcggttga ttttagttat attttaaaaa atggcgatta tgtttctgtt tatccggttt
    23821 ttgagagcct tgacatttca ttggtttccc ggcttcgaaa tgcgccattg cgtcaggtaa
    23881 aatttatatt agatgtgcat ttagggaaac tatgtaaata cctgcggata ttaggattcg
    23941 atacatatta tcggaatgat ctggaagata gcgaaatagt ttccatttcg gtaaatgaaa
    24001 acagaataat cctaacgcgt gatattggga tactgaagaa tggaaaggta acgcatgggt
    24061 attgggttcg ctcccaaaat tcgaggcagc aattatggga ggttattcat agatttgatt
    24121 tggaaaacat tttacagccc ttttatcggt gtattagttg taacggactg attcatccgg
    24181 tagaaaaaga aaaaatagag catctgttgc gagaaaacac aaaacgattt tttacagatt
    24241 tttatcagtg taacgcttgt ggtaaagtat attgggaagg tacgcattat gaaaatatgc
    24301 aggagtttgt gaataatatt atatcaagaa aaaaactgct aataagttaa atgttgccta
    24361 tttttgattt taaaatttaa gttttcgatg catcttaaaa gacctacttt attgattgat
    24421 aaacaaaagt gcttggccaa tatccggttg tttgtcgtca aagcagttaa gtacaattta
    24481 cgattccgcc ctcatttcaa aactcaccaa tcggttgagg ttggtagctg gtttagggag
    24541 tttgggatag ataaaataac ggtttcgtcg gtgatgatgg ctgagtattt tgcaaaaaac
    24601 ggttggaatg atatcaccat cgctttccca gtgaatattg cagaaactga cgagatcaat
    24661 attttagcaa aacagattaa attaaacctt ttagttgaaa atgttgaggc tgtcggatca
    24721 ttatcccgca cactgaaaaa tccggtgggt gtgtatgtaa agattgacac cggttatcat
    24781 cgtagtggca ttgaagtgga aaagtatgat gccattgctg atctgattaa tattattaga
    24841 agggcagaaa atttgaattt taaaggatta cttgctcata atgggcttac ctacttacaa
    24901 tcaacgcctg atgcgatttg taaagtgcac gatgaaagcc tgacaaagct gaaaaggttg
    24961 aaagattctt tgaaaaaagc aggt
//
LOCUS       DAOOAI010000019        24834 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_2630_length_24834_cov_5.119335, whole genome shotgun sequence.
ACCESSION   DAOOAI010000019 DAOOAI010000000
VERSION     DAOOAI010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24834)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24834)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24834
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2630_length_24834_cov_5.119335"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1408
                     /locus_tag="PK345_02560"
     CDS             <1..1408
                     /locus_tag="PK345_02560"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33934.1"
                     /translation="SHVIRYRTNWSQPVTDPCNTYSYGNCCDFTVQIGGVPPQSWLTA
                     EPMSGTLSPGQSGTVKVKFNSAGLDFGTYTGSVIFNNNSSTPVVTVPVTLIVEQGCPL
                     PPPTDLTLQQTGYNPNVVQLSWVAPEEPGGVIRWDNGINNDGIGLVNGGTFAVAAKWN
                     PDQLTQHVGLYLKQVDIFPRSATATYIIKVWTSATVSQPVVSQSATVVSEQWNTITLT
                     NPVQIEAGKPLYIGYETTHGVSEYPAGCDSGPAIANFGDLISTGGGAWVSMSTQFGLD
                     YNWNIAGIIGIGTDGAAPAVPIVITPNPDVSYGSPVAGNLGKPNNAKWVNTSRALVGY
                     DIKRDGQVIGSVGPTVTTYTDSNVQGGTRIYNVGAHYTECTAWSDPATINVFVTELSP
                     DATTLRIYPNPASETVNIESTTIKQIIIVNNLGQVVYDSLFNNDYVQINTSNLRKGMY
                     VIFIRTAGTTFTEKLIIK"
     gene            1498..3441
                     /locus_tag="PK345_02565"
     CDS             1498..3441
                     /locus_tag="PK345_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007853274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPX33935.1"
                     /translation="MLSINNISVHFTGEYIFDSVTFLINDRDRIGLVGRNGAGKTTLL
                     RVISGELEPETGSVASPAGQSIGFLRQEMTPDSKLTVIQEARQAFKEVLAIESKIKRL
                     TNEISNRTDYHSESYLNLVERLNDLNDRFNLTDGHKIDENTEKVLIGLGFEREDFSRP
                     LREFSSGWQMRVELAKILLTMPDVLLLDEPTNHLDIEAIQWLEDFLISYPGAIVLVSH
                     DRVFLDNITNRTIEITMGKIFDYKASFSQYEQMQAERREREMASYNNQQQQIAQIERF
                     IERFRYKNTKARQVQSRIKMLDKMDKIEIEETDNSAIRLRFPPSPHSGRVTLRLENLS
                     KAYGEHLVLQNLNFSISRGEKIAFVGRNGEGKSTLAKVIVGELPYSGTLIYGHLVKIG
                     YYAQNQHEMLDMERTVFETIDDVATGEWRTKVKGLLGSFLFRGDDLDKKVKVLSGGEK
                     SRLSLARMLLTPVNFLILDEPTNHLDMRSKDILKSALLQFDGTLIIVSHDRDFLSGLT
                     EKVFEFHNKGVKEYIGDVHDFLQSRKMEHLNQLNQKAKTQSLVEETNTPSASKLDFER
                     RKAIDRELRKLQNRLEKSEKIIIRLESDLAEFDALLAKPDSSHPLIKSGEIYHKYEIL
                     KNELAKEMERWETLVHQLENVQQ"
     gene            complement(3571..6057)
                     /locus_tag="PK345_02570"
     CDS             complement(3571..6057)
                     /locus_tag="PK345_02570"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPX33936.1"
                     /translation="MKKLLFIFLLLTNTIIYCQDTITVMQYNLLNFGYFTSFCTVNNN
                     NPDSKTLWLKSIVDHYLPDLLSVNEISPNTIYHFKILNEALNASGRDYYQKAESTNFA
                     GSTIINMLYYNSLKIGLAEQEVIFTTLRDINVYKLFHKSAYLVSGSDTTFFYYITAHF
                     KSGANAANQQLRAEMAEAVISYLEENNISEPVMLGGDLNLYSSSESAWTILTDTNKNC
                     YFNDPLNRVGNWSSNPEFADTHTQSTQTTSGCGAGGGMDDRFDFVLTNSSLTTESYPV
                     NIIPDTYQLPGQDGLRFKGSLIDPPNTSLPAELIDALYNISDHLPVTLKLIVRTPQPN
                     YVPDLFFSEYVEGSGNNKALELFNPTPYPKDLSNYRLERYVNGSVYADTVSLAGTLPS
                     GKTYVVVIDKRDPNGSGANTPAHPDLIAVADTFLCPDPTINQMMYFNGNDAIALRTQS
                     GELIDLIGKIGEDPGTGWTDDSLCYPGPYTSLCGAKAWTTNHTLVRKFNVTSGIKTNP
                     PFFDVTQQWDSLPNNTFDSLGLHHCLTQFELPPSWEYVTTMSSHIFTITINTNINLED
                     QPAAPGLFIGAFFKNGDSIHCAGNVQWFGDQNIAIIVYGDDFLTPEKDGFEINEKITW
                     KILVPSLMKEFDAAATYSSFWPQNNGLFTPNGISEIISLNGKEITNFSLYVSEGWSGV
                     SLPINATWPLLEDIFGLEINNVVVMSDNEHIFYPEQNINQIVEWQEGVGYYIKANLEF
                     LKNIVGFSPSSKTVQLNSGWNIIPVMSFDDIPVSLIDIELGSKLKLIKEVAGTAIYWP
                     EINIQTLEKLSPGKSYFIYLNESGSFSFGE"
     gene            6193..7599
                     /gene="glmM"
                     /locus_tag="PK345_02575"
     CDS             6193..7599
                     /gene="glmM"
                     /locus_tag="PK345_02575"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HPX33937.1"
                     /translation="MSLIKSISGIRGIIGGRPGENLTPVDVVKFTAAYAMFLKKNIKN
                     SKLKVVVGRDARVSGDMLNSLVTSTLNAMGIDVIDAGLSTTPTVEIAVVESGANGGII
                     LTASHNPGQWNALKLLDEHGEFLSQMAGEEILVIAENEQFHFSPYNELGKTVLEHGFI
                     DLHIQKIISLPMVDAAAIRNAGFVVAVDAVNSTGGLAVPKLLEELGVERIEKLYCEPT
                     GLFPHNPEPLPENLTELAKFVVKKRCNIGFAVDPDVDRLAIINEDGTAFGEEYTLVAV
                     ADYILRTKPGNTVSNLSSTRALMEVTHKAGGNYMASAVGEVNVVKMMKETNAVIGGEG
                     NGGVIYPEIHYGRDALVGIALFLTFMAKTGKKPSQIRQFYPNYFISKNKITLEPDMNV
                     DQILERMALKYSKYKVNRIDGVRIDFENDWVHLRKSNTEPIVRIYTESNSEAKANHLA
                     QKMITDIGEIVNSPEVED"
     gene            7772..8035
                     /locus_tag="PK345_02580"
     CDS             7772..8035
                     /locus_tag="PK345_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33938.1"
                     /translation="MLAKTKKKRKITKFKSITFKLSTRQKKSLDKNCRVRKTTPIKLI
                     KKSIDAYLSLRINEDPVAYVSPNQLNLFSDADFEVEENDDVDE"
     gene            8070..8255
                     /locus_tag="PK345_02585"
     CDS             8070..8255
                     /locus_tag="PK345_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPX33939.1"
                     /translation="MDLSSLYTAGFLGPTEIILIVLVVLLLFGGKKIPELMRGLGKGI
                     KEFKDGMNEGDKSEKKE"
     gene            complement(8259..11633)
                     /gene="mfd"
                     /locus_tag="PK345_02590"
     CDS             complement(8259..11633)
                     /gene="mfd"
                     /locus_tag="PK345_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HPX33940.1"
                     /translation="MTVEEIITRYQRSAEIAAIHASLINELPTRLYISGLKASQPSFV
                     AAAILKLLNKTHIFVLPEKESAAYFHNDLETIFEEKEKKYAERKILFLPTSYKRPFKL
                     DEPDSSNILLRSETLNKIIDTRNPLAIITYPEALAEKVPDRITTGQNSLKVKKGDEIS
                     FDFMTDFLIEMNFERVNFVVEPGQFAVRGGIIDVFSFSDDHPFRIEFFGNEIESMRTF
                     DTANQLTLAFLSEITIMPDLHKTTDKISKIPFIKYIDQNSIFWFENLNYTVEKIESEC
                     KKLNETYLFNDNKGRVSEICFGGQELLANLNDFHVIEFGARCLMQPDKTIEFQAQPQL
                     SFNKNFDMLITKLNENSVNNIDNFIFTVDLKQAERLHTILDDIQSKNETSRHTQYYTI
                     ARPIHEGFTDFTCRVACFTDHQIFDRYHLFHIRKATYGREALSVKELYNLQPGDFVTH
                     IDHGVGRFDGLETIENNGRQQEAIRLVYQDGDLLYLSIHSLHRIAKFIGKEGIAPSLN
                     KLGTNNWNRLKERTKKKVKDIARDLIKLYSARRASNGFSFSPDSYLQHELEASFIYED
                     TPDQLKATIDVKADLEKNFPMDRLICGDVGFGKTEVAIRAAFKAVTDSKQVAVLVPTA
                     ILALQHFKTFSNRLNDFPVNVEYINRFKSTREQKQIIQELEAGRIDILIGTHRLVSND
                     VKFKDLGLLIIDEEQKFGVSIKEKLKKLKVNVDTLTLTATPIPRTLQFSLMGARDLSV
                     INTPPANRIPVITELHSFSSEVIKSAINFEVARGGQVFFVHNKVQNINEVAEMVRIFC
                     PRVKVGIGHGQLQGAKLEQVMLDFIEGDYDVLVATTIVENGLDIPNANTIIINNAQNY
                     GLSDLYQLKGRVGRANRQAFCYLLSPPLSTLNQKARKRLKAIEEFSSLGSGFSVAMRD
                     LDIRGAGNILGAEQSGFISEIGFEMYNKILDEAMEELEEETFANDSEKLINKSYVKDC
                     LLETDFELMIPDSYISITTERLSIYKELDNIKTEDGLHKIKNQLIDRFGPIPEQVEGL
                     FSAIKLRWIASRIGFEKIVLRGGDMTGYFTGTEDSAYYNSMAFTHVIDFLKNNPKTCS
                     MRESKQKLSLKFTKVESVEIALKKLQPLIYYPDEEN"
     gene            11780..12319
                     /locus_tag="PK345_02595"
     CDS             11780..12319
                     /locus_tag="PK345_02595"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPX33941.1"
                     /translation="MVNILEVNHEVVLHQVSYSDAPALFRLIDNNRTSLRAWLPFVDF
                     TKVVEDTEAFIGSLFVPGSRNLVFTIRYRGEVAGLIGYKDIDRNNKKLEIGYWIAPVF
                     EGNGIVTQSLEVLIGAAFSYLEMNRIQIKCAVGNQRSINIPKRLAFRFEGIERAGEWL
                     NDRFVDLEVYSMLRSDWIK"
     gene            complement(12355..13476)
                     /gene="lpxB"
                     /locus_tag="PK345_02600"
     CDS             complement(12355..13476)
                     /gene="lpxB"
                     /locus_tag="PK345_02600"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008915 - lipid-A-disaccharide synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HPX33942.1"
                     /translation="MKYYIIAGEASGDIHAANLIKEIKFLDKQAHLRAWGGDRMYDQG
                     VELVKHYHELAFMGFYEVIVNLKTILQNIKFCKQDIIRFKPDVLILIDYPGFNLRIAK
                     FARKHGVRVFYYISPQIWAWRQSRVKKIKKYVNRMYVILPFEKDFYLKHNIEVDYVGH
                     PLLDVIENEKANYHDLISFKDKNNLENKPIIALLPGSRKQEIAKMLPTMLTIPDHFPE
                     YQFVIAGTNAVENDFYKRSLNEKAVKVIYQQTHQLLRHAEAAIVTSGTATLETALMNV
                     PQIVCYKGNWFSYQIAKRVIKTKYISLVNLILNKPVVTELIQKEMNSSNLHTELKNIL
                     NPEIRQRILNDYSQLHKVLGSEGASKQVAKLMVNRLLEY"
     gene            complement(13477..13764)
                     /locus_tag="PK345_02605"
     CDS             complement(13477..13764)
                     /locus_tag="PK345_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33943.1"
                     /translation="MKQFWHFLNKDSYLFGIILGICTPVVLYVFILGIVELIIHFHFT
                     INSPNKLKLLATAGNLIWIRYYFVVKKSDKTGFAVLAITFILIISYFIFYK"
     gene            complement(13774..14604)
                     /gene="surE"
                     /locus_tag="PK345_02610"
     CDS             complement(13774..14604)
                     /gene="surE"
                     /locus_tag="PK345_02610"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HPX33944.1"
                     /translation="MANTKPTILVTNDDGINAYGLRTLIKIMRNLGDVVVVAPDRPRS
                     GQGHAITVTMPLRVTQIHQEEGYSEYSCNGTPVDCVKFGVQIILRRQPDLVVSGINHG
                     SNASVNVFYSGTMAAVIEAAIDKIPAIGFSLDDYDSNANFSHIEKYVEKISSEVLRKG
                     LPFNTCLNVNFPKLQDEPIKGVKVCRQSLGSWKEAFDERTDPYGGNYYWITGIFSNAD
                     HKEGTDTWAMEKNWVSVVPMKFDLTNHESIKLLSEYNFDFGHNNKTLLETKSATTYNK
                     "
     gene            complement(14618..15205)
                     /locus_tag="PK345_02615"
     CDS             complement(14618..15205)
                     /locus_tag="PK345_02615"
                     /inference="COORDINATES: protein motif:HMM:NF016270.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HPX33945.1"
                     /translation="MKSKTIISRLIRFFLQGLLFTGPIAITIYSIWLVFNWIDGILYK
                     YIENFIGRPIPGLGLILVLAVITLVGFLGSSFLFKPLAIYFDGLLSKTPLVKIIYTSV
                     KDLIAAFVGQKKRFNQPVLVKVNAEGTIEKFGFVTDEDLSSIGVSEEKIAVYLPHSYA
                     WSGNLFIVPAKNVTRINASATDVMKYIISAGVTKV"
     gene            complement(15369..15944)
                     /locus_tag="PK345_02620"
     CDS             complement(15369..15944)
                     /locus_tag="PK345_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HPX33946.1"
                     /translation="MLKTKFHIILASNSPRRQQLIESMGLDFEIRIKPVDENFDTSLT
                     PEEVALYLCSKKACAFNLEDIGKDNLIIAADTIVCLDNRILSKPKSREHTIEILGELS
                     GKTHLVITGVALRTKEKMITFTETTEVVFEKLTLKEIEYYTDRFKPFDKAGAYGIQEW
                     IGMIGIKSIKGSYFNVMGLPTARLWRELKSF"
     gene            complement(16070..17290)
                     /locus_tag="PK345_02625"
     CDS             complement(16070..17290)
                     /locus_tag="PK345_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ectonucleotide
                     pyrophosphatase/phosphodiesterase"
                     /protein_id="HPX33947.1"
                     /translation="MTNKVIFFLAALLLLQTSCKQSKTTNDARYLIVLSMDGFRKDYT
                     EKSNTPNLDSIALIGVRAEGILPAFPSKTFPNHYTMATGLYPDHHGIVNNSFMDPATG
                     LAYKMKDSAARLNPYFYGGEPIWVTASKAGLKTASFYWVGSELPIKDIQPDYWKKYDE
                     SVSFEDRIDTLIYWLKLPKKNRPQLIMCYFHEPDAIGHKFGPESNETILMVEYIDKLI
                     GDLCRKINMLPYAKKIDLIFTSDHGMAQLSEDRVINMREIIPDEWLETTMGENPVFNV
                     GAKQGYLDSVFNRLQKTEHVKSWKNSDMLERYHYGTNPRTLDITVEADLGWTINWRKS
                     NNYLYAQGTHGYDPDYPEMHAVFYAVGPSFKKGYLHPAFDNVNLYVLMAEILGLEPAP
                     NDGDLEKVINMLNR"
     gene            complement(17491..20775)
                     /locus_tag="PK345_02630"
     CDS             complement(17491..20775)
                     /locus_tag="PK345_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009036940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPX33948.1"
                     /translation="MSKKLNFLAVSLLLLFTTALHAQITTSSMTGRVTDTQGTIVGAT
                     IVATHIPSGTTYGTITNVDGRFILNGMRVGGPYKVEISFVGYGKNIIDDIILSLGETY
                     VLNVKMQEEMIELKEFVVSAEKTRFTTERTGATTNISNQQMNSMPTITRSVQDIARLS
                     PFANGMSFAGSDGRSTNFTVDGANFNNNFGLSSNLPGGGNPISLDAIEEVQVVVAPFD
                     VRQTNFIGGGINAITKSGTNEFKGTAYTYYKNQEMRGNRIDEIDFGIRNIESTKTYGI
                     SLGGPIIKNKLFFFVNGEYELKPGQVVNWRPSEDGVANTDLMLSRTSKSDMEKVKQHL
                     LTNYGYDPGSYTDYPADESNKKLMARIDWNINNSNKLSLRYNFTKNQAWYQTNGNSTD
                     AGYRLSGLNRISQYSMAFSNSIYSQENTVNSLALDLNSRFSNKVSNQFLTTYTKIKDM
                     RGSNSSPFPFIDIMYGRNDDGTQILEPYISAGYELFTWNNGVNNNIFNIIDNVNVYLN
                     NHKVTIGASFEHQMANNSYMRNGTGYYRYSSLDDFLNQATPTDFAVTYGYGGEKNPAA
                     EVAFNQIGIYAQDEWNVNPDFTLTYGVRGDFLAYEDNIMSNNAISELDFGGKKIDTGK
                     WPDAKLQISPRVGFTWDVKGDQKMKLRGGTGLFAGRLPLVFFTNMPTNSGMVQGSYAA
                     VTKFDANGNVISADPKLAMLAGKMITDVNEMISILGLPNEITPEQGVLPRDVNSVDPD
                     FKMPQVWKTSLALDYDLPASFPLSVTIEGIFTKTINGVMLQNWDLKEPDDTWQRFSGP
                     DDRYIYPAYADLAYNKKNSYVLTNTNEGWGAIGNISIFAEPVKDLSLMLAYTYTESKE
                     ISGMPGSNAASAYTGLMEIDGPHLPILQRSQYVVPSKLIGSAFWKIPWGNNSLKSATT
                     VNLFYTGFTPYGNTFAYTNDMNGDGIGNDLIYIPKNKGDIKFITPEDEDAFFKFMDQD
                     KYLSKHKGEYAQAFAARAPWVNRFDLRLAREYYFNVAGKRNTLQFSLDCLNFGNLINS
                     KWGVYKSNTVSNNGRILKYEGKDANNVPEFSFVKVKGEYPTETYDYNYNYTQAWQLQF
                     GIRYIF"
     gene            21043..22449
                     /locus_tag="PK345_02635"
     CDS             21043..22449
                     /locus_tag="PK345_02635"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX33949.1"
                     /translation="MHDERDSIDGELDELIGRFDEMVRHDKLYFFDVDEFEEIIDFYF
                     EHHNNRRVQVAIDHALRQHPSSHIFLLRKAQYLADINKLGKALEILQKLELLEPQNPD
                     VYLTLGTVYSKLKKIKQAIDAYYKAIEYSDEKDDIYIRIAFEYEELNDYDKAIKYLIK
                     ALDFNPNNQSVIYELSFCFELAGRVEDAIAFFNEFLDKNPYSDFGWFNLGIAYSTIGL
                     FEKAVDAYDFVIAITPDFSSAYFNKANALVNLDKYAEAVETYKEVLKYEEPEAVTYFY
                     IGECYEKLEHYSEAFDYYRRSSKLNPEYAEAWMGMGSIHDKMGNESAAIRYYKKAVEL
                     DEYDTENWLTLGDAYYRYDKFDKAKDAFRKACEVEPLHPDVWLDYSAMFAEEEDFSNA
                     ITIMIKGIEHQPQNAEFYYRLAGYLMMKGREKEAILNFEIAVQINYDDYYLLFDFYPE
                     LADNARFNELLNIYKPLK"
     gene            22491..23423
                     /locus_tag="PK345_02640"
     CDS             22491..23423
                     /locus_tag="PK345_02640"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HPX33950.1"
                     /translation="MQKYFYFVLKGLGMGAANVIPGVSGGTIALITGIFERLINAIKS
                     FDITALKLFFTGRFKDFARHVDLAFLVAVFFGVVLAIISVARLFEYLFANYPIYIWSF
                     FFGLIVASVYFVGRTISKWSLTVIIVFVAGTSLAVLLTFLKPAAENDNLLYLFICGIV
                     GACSMILPGISGSFVLILMGNYQLVMINAVNQMNVSILAPVVLGAIFGLIAFSNFLSW
                     VFKKFPDYTIASLTGFILGSLGIIWPWKNNVYQIDVNGAFALNRKGEKIVTGYEWFLP
                     DSFTTEVVIALGFIVLGFLTIWIIEYLAGKRVNK"
     gene            23420..24166
                     /locus_tag="PK345_02645"
     CDS             23420..24166
                     /locus_tag="PK345_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HPX33951.1"
                     /translation="MKRFGIIGKKLSHSFSADYFNKKFEKEGFGDCSYEMFELNEPNE
                     ISKLVETNNDLIGVNVTIPFKTNIIPFLDALDPVAKQVGAVNTITVNRIGDRVKLVGY
                     NTDIYGFELSTNAFETYKNAVILGTGGAAKAVSHVLKKKNINHLFVSRKPTSENEIGY
                     KDIDENVLRRFLWIINATPLGMFPDIDSFPPIPYHHLTSRHFLYDLIYNPSDTLFLKK
                     GFEAGAKTKNGLDMLHLQAEITWVIWKKMI"
     gene            24321..24674
                     /gene="rplS"
                     /locus_tag="PK345_02650"
     CDS             24321..24674
                     /gene="rplS"
                     /locus_tag="PK345_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015547020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HPX33952.1"
                     /translation="MSKNQILSYVEGLTPVTVMPDFKAGDTITVKYKIREGNKERIQN
                     FAGVVLQRKGQGPTATFTVRKISNNIGVERIFPVSSPFIEEIVVNKRGVVRRARIFYL
                     RGLRGKKARIREKRV"
BASE COUNT         7231 a         4450 c         5135 g         8018 t
ORIGIN      
        1 gtcgcacgta atacgttaca gaaccaactg gagccagcca gtaactgatc catgtaatac
       61 atatagttat ggcaactgct gtgatttcac agtacagatt ggtggtgttc caccacaatc
      121 atggctgacc gcagaaccaa tgagcggtac tcttagtcct ggccaatccg ggaccgttaa
      181 ggttaaattc aattctgcag gtttggattt cggtacatat accggttcag ttatctttaa
      241 caataactca tcaacacctg ttgttactgt acctgtaacc ttaattgttg aacagggctg
      301 tccgcttcct ccacctacgg atctcacatt gcaacaaact ggatacaatc caaatgttgt
      361 acaactctcg tgggttgcac ctgaagagcc gggaggggta atacgttggg acaatggaat
      421 aaataacgat ggtattggat tagttaacgg tggaacattt gctgtcgctg ctaaatggaa
      481 tccggatcag ctaactcaac atgttggcct ttatcttaag caggttgata ttttcccaag
      541 gtcagctact gctacataca ttattaaagt gtggacaagc gctactgtat ctcaacctgt
      601 cgttagccaa tcagctaccg ttgtatccga gcaatggaat accattactc ttactaatcc
      661 tgttcagatt gaagctggca aaccgctcta tattggttat gagacgactc atggtgtaag
      721 cgagtatcct gctggttgtg attcaggtcc tgcaattgcc aactttggtg atttaatcag
      781 tactggtggt ggtgcatggg tttcgatgtc aacacaattt ggtctggatt ataattggaa
      841 tattgccggt ataattggta ttggtactga tggtgcagcg cctgctgttc cgatagttat
      901 cacaccaaat cctgacgttt cttatggttc accggtagca ggtaatttag gcaagcctaa
      961 taatgcaaag tgggttaata cctcaagagc tttagtgggc tatgatatta agagagacgg
     1021 tcaggtgatc ggttctgttg ggcctactgt tactacctat accgatagta atgtacaagg
     1081 aggtacgcgt atctacaatg ttggagctca ttatactgaa tgtactgcat ggtctgatcc
     1141 agctaccatt aacgtatttg ttaccgagct ttcgccagat gcaactacct tacgtattta
     1201 tccaaatcct gcttctgaaa cggttaacat cgagtcaacc actattaagc aaattataat
     1261 agtcaataat ctcggtcaag tggtttacga tagtctgttc aacaatgatt atgtacaaat
     1321 aaatacctct aacttacgta aaggtatgta cgtcatcttc atccggactg ccggaacgac
     1381 atttactgag aaactgatta tcaaataatc cttaattgga ataatcctaa aaaggccggg
     1441 ccatttggtt cggccttttt tttataattt ggctattttt gtttcaaatt ttgtatagtg
     1501 ctttcaataa acaacatttc ggttcatttt acaggagaat atatttttga tagtgtcact
     1561 tttcttatta atgatcgtga taggattggg cttgtaggcc gaaatggcgc agggaaaaca
     1621 acattgttaa gggttatttc cggagagctt gaacctgaaa ccggctcagt agcatctcca
     1681 gccggccaaa gcattggctt tttgcgacag gagatgaccc cggatagtaa actcactgta
     1741 attcaagaag cacggcaggc attcaaagaa gtacttgcca ttgaatcaaa aataaaaaga
     1801 cttacaaatg aaatttccaa ccgtactgat tatcactccg aaagctatct taaccttgta
     1861 gaacggctaa acgacctcaa tgatcggttt aatttgacag atggtcataa aattgatgaa
     1921 aataccgaaa aagtcttgat aggacttggg tttgaacgtg aggatttttc gcggccgttg
     1981 cgcgaattta gtagtggatg gcagatgcgt gtagagcttg ccaaaatatt gcttacaatg
     2041 cctgatgtat tgctacttga cgagccaaca aaccatctcg acatagaggc gatacagtgg
     2101 cttgaggatt ttttgatcag ttaccccgga gctatcgtgt tagtttcgca cgatcgtgtt
     2161 tttttggata atataacaaa ccgtaccatt gaaattacaa tgggaaagat ttttgattat
     2221 aaagcctctt tttctcaata tgaacagatg caggctgaac gccgcgagcg cgaaatggcg
     2281 agctacaata atcagcaaca acaaattgca caaattgaac gcttcattga acggtttcga
     2341 tataaaaaca ccaaagcacg tcaagtgcaa tcgaggataa aaatgctcga taagatggat
     2401 aaaattgaga ttgaagaaac cgataattca gccatacgat tacgcttccc accctcgcct
     2461 cattcaggaa gagtaacatt gaggctcgaa aatctttcaa aagcctatgg tgaacacttg
     2521 gtattgcaaa atcttaactt ttctatctca cgaggagaaa agatcgcatt tgtagggcga
     2581 aatggagaag gaaaatctac cctcgcaaag gtgattgttg gagaattgcc atatagcggg
     2641 actttaatat atggacacct tgtgaaaatt ggatactatg cccaaaatca gcatgaaatg
     2701 ctcgatatgg agcgaactgt ttttgaaact attgatgatg ttgcaacagg agaatggaga
     2761 acgaaagtga agggtttgtt gggtagtttt ttgtttagag gggatgactt agataagaag
     2821 gttaaggtgt tgtcgggtgg ggagaaatca aggctttcgc ttgctcgtat gttacttaca
     2881 ccggttaact ttctgattct tgacgagcct actaaccatc ttgatatgcg ttcgaaagac
     2941 attttaaaga gcgcgctttt gcaatttgac ggaacattga tcatcgtttc gcacgatcgt
     3001 gattttttgt cgggattgac agaaaaagta tttgagtttc acaataaagg agttaaggag
     3061 tatatcggag atgtgcatga ttttttgcaa tcgcgaaaga tggagcatct caatcagtta
     3121 aaccaaaagg ctaaaacgca atctcttgtt gaagaaacaa atacgccttc ggcaagtaag
     3181 ctcgattttg aacgaaggaa agcgatagac cgggagttgc gaaaacttca aaatagatta
     3241 gagaaatcag aaaaaattat catccgactt gaatctgatt tggctgaatt tgatgctctg
     3301 cttgctaaac ccgactcttc tcatcctctt ataaaatccg gtgaaatata ccataagtat
     3361 gaaattctta agaatgaact ggcaaaagag atggagaggt gggaaacgtt agtgcatcag
     3421 cttgagaatg ttcaacaata gcattccatt aaaaatcatt ttgttgtaaa atacaataat
     3481 tgatcgttct tcaaattata aaataagatt aatctttacc tttttataaa atctattcaa
     3541 gtaatagggg gttaaagtaa taaaggtaag ctactcaccg aaagagaaac tgcccgattc
     3601 gtttagatag ataaagtacg acttgcccgg cgatagtttt tcgagcgttt gaatattgat
     3661 ttcaggccaa tagatcgctg tacccgccac ctctttaatc aattttaatt tactaccaag
     3721 ctcaatatca ataagcgaga caggaatgtc atcaaagctc ataactggta taatgttcca
     3781 tcccgagttg agctgcacgg ttttactgct tggactaaag ccgacaatat tttttaaaaa
     3841 ttccaaattg gcttttatgt agtatcccac gccttcctgc cattcgacta tctggttgat
     3901 gttttgttca ggataaaata tatgttcatt atcgctcatc actactacat tgtttatttc
     3961 aagtccgaaa atatcctcta atagtggcca tgtggcatta ataggaagcg aaacccctga
     4021 ccagccttcg gatacgtaca atgaaaagtt ggttatctct ttaccattca gtgatataat
     4081 ttctgaaata ccattaggcg tgaaaagccc gttattctga ggccagaatg atgagtaggt
     4141 agccgcagcg tcaaattctt tcataagaga aggaactaaa attttccacg ttattttttc
     4201 atttatttcg aaaccatctt tttcgggcgt taaaaaatca tctccatata caataattgc
     4261 aatattctga tcgccgaacc attgtacatt gccggcacaa tgtatagaat ccccattttt
     4321 gaaaaatgcc ccgataaaaa gtccgggcgc agcaggttga tcttccaaat ttatgtttgt
     4381 attaatggtt attgtgaaga tatgagaact cattgttgtt acgtattccc atgatggcgg
     4441 cagttcgaat tgggttaagc aatggtggag tcccaggctg tcgaaagtgt tattgggcag
     4501 tgaatcccat tgttgtgtaa catcgaaaaa aggaggatta gttttgattc cgcttgtaac
     4561 attaaatttt cgtactaacg tatggtttgt tgtccatgct ttggcgccac aaagtgaagt
     4621 ataaggtcca ggataacata atgaatcgtc agtccagcct gttcctgggt cttcaccaat
     4681 ttttccgatc aggtcaatca actctcctga ttgtgttcga agcgcgatag catcgttgcc
     4741 gttaaagtac atcatttggt tgatcgtagg atcggggcaa aggaaggtgt ctgcaacagc
     4801 tatcagatca gggtgtgccg gtgtgttggc tcctgatccg tttgggtctc ttttgtcaat
     4861 aacaaccacg tatgtttttc cggaaggtag tgttcctgcc aggcttacgg tgtcggcata
     4921 aacagagcca ttaacatatc tttctaaccg gtaattagat agatctttcg gatagggtgt
     4981 gggattaaaa agttcaagcg ctttattgtt gccgctacct tcaacatatt cggagaaaaa
     5041 cagatcggga acataattag gctgtggcgt tctgactatc agcttcagag taaccggaag
     5101 atggtcggat atgttgtaca gggcatcaat cagttcggcg ggcagacttg tgtttggcgg
     5161 atcaatgagt gaacctttaa aacgtaggcc gtcttgccct ggtaattggt aagtgtcggg
     5221 gatgatgttt acaggatagc tttcggtggt aagtgatgaa tttgtaagta cgaagtcgaa
     5281 acggtcgtcc ataccccctc cggcgccaca tcccgaggta gtttgggtag attgagtatg
     5341 tgtatcggca aactccggat ttgagctcca attaccaact ctgttaagcg gatcgttgaa
     5401 atagcagttt ttattagtat cggtcaaaat tgtccaggcc gactcgctgc ttgagtataa
     5461 attaaggtcg ccaccaagca taaccggctc tgaaatgtta ttttcctcca aatagcttat
     5521 aactgcttcg gccatttctg ctctaagttg ttggtttgcg gcattagcgc cagatttaaa
     5581 atgagctgta atgtaataaa agaaagtagt atccgagccg gaaaccaaat aagcgctttt
     5641 atgaaataac ttataaacgt tgatgtctcg tagagtggta aaaattactt cctgttccgc
     5701 taatcctatt tttaaagagt tataataaag catattgata atagtagaac cggcaaaatt
     5761 ggtggattca gccttttggt aataatctct acccgaagca tttaaagcct cattgagtat
     5821 tttaaaatga tagattgtgt tagggcttat ttcatttaca gagagtagat caggtaaata
     5881 atgatctacg atactcttta gccagagggt tttgctgtca ggattgttgt tgttaacagt
     5941 acaaaaactc gtaaagtacc cgaaattcaa taagttatat tgcatcacgg taattgtatc
     6001 ctgacaatag attatggtat tagttaacag taaaaaaata aaaagcagtt ttttcatcga
     6061 atgaagtgtt tgagttagcg aagttaaacc aattttgtgg aagtcggttc aaagtattga
     6121 taaacaaaaa aagctctttc ttttaatgca aagtgaattt agggttattt ttgcccggaa
     6181 attttagtga ttatgtcttt gataaaatct atatcaggaa tacgtggcat aatagggggg
     6241 aggcctggcg aaaatcttac tcccgttgat gtggtaaagt tcacggcagc ctatgccatg
     6301 tttctcaaga aaaatattaa aaattcaaag ttaaaagttg tcgtagggcg tgatgctcgt
     6361 gtttcaggcg acatgctaaa tagccttgtt acctcaacat taaacgccat gggcattgac
     6421 gtcattgatg ccgggctttc tactactcct acggtggaaa tagctgttgt agaatcaggc
     6481 gctaacggcg gtatcatttt aacggcaagc cacaatcccg gtcaatggaa tgctctgaag
     6541 ctgcttgatg aacatggaga gttcctatcg caaatggcag gtgaagagat acttgttatt
     6601 gcagaaaatg agcagtttca tttttcgcct tataatgaat tgggcaaaac cgtgcttgag
     6661 catggtttta ttgatcttca catacaaaaa attattagcc ttccaatggt tgatgctgct
     6721 gctattcgta atgcaggttt tgttgttgca gtagatgcgg taaactctac cggaggtctg
     6781 gcagttccta aattgctcga agagcttggt gtggaaagaa tcgaaaaatt atattgcgaa
     6841 cccactggtt tatttcctca taaccccgag cctttgcctg agaatcttac ggagcttgcc
     6901 aagtttgttg taaagaaaag gtgtaatatt ggttttgctg ttgatccgga tgtggatcgt
     6961 cttgcaatta ttaatgagga tggtactgcc tttggcgagg aatacacttt ggttgctgtg
     7021 gctgattata tattgaggac aaagcccggt aacaccgtat caaatctttc gtcaacgaga
     7081 gctttgatgg aagttacaca taaagcgggt ggaaattata tggcttcagc agtaggtgag
     7141 gtgaatgtgg ttaagatgat gaaggaaact aatgctgtta ttggcggaga aggtaacggt
     7201 ggagttatat atccggagat tcattatgga cgcgatgcat tagtggggat tgctcttttt
     7261 ttaacattta tggctaaaac aggcaaaaag ccctctcaga taaggcagtt ttatcctaac
     7321 tacttcattt caaaaaataa aatcaccctc gaacctgata tgaatgtaga tcagatattg
     7381 gaaaggatgg cgctcaaata ttcaaaatac aaagtcaata gaattgatgg cgttcgtatt
     7441 gatttcgaaa atgattgggt gcatcttcgc aaatccaata ccgagccgat agtcaggatt
     7501 tatacagaaa gtaactcgga agccaaagct aatcatttag cgcaaaagat gataacagat
     7561 attggagaga tcgtaaactc gccggaagtg gaagattaga acttcaaacc tgaatcaata
     7621 gaaagttctc tttggatgat tattaataga taattattta agtattagtt agatacttaa
     7681 cctggattta tttaaaacca ctaataaatg tttgacaaga ctttgatata atgaaaagtt
     7741 agtattttta tacaaatttt tacatttcat tatgttggca aaaacaaaga aaaaaagaaa
     7801 gattacaaaa tttaaatcca tcacttttaa attatctaca aggcagaaga agtcgttgga
     7861 taaaaactgc cgtgtacgaa agacaactcc gatcaaattg ataaaaaaat caattgatgc
     7921 ttatttatcc cttaggatta atgaggatcc tgtggcgtat gtttcaccta accagcttaa
     7981 tcttttttct gatgctgatt ttgaggttga agaaaatgat gatgtggatg aataaataaa
     8041 tctatctttg tttaaaaata actaatgcca tggatctttc aagtttatac acagcaggtt
     8101 ttttagggcc taccgaaatt atccttatcg ttttagtagt cttgttgctt tttggcggca
     8161 aaaagatacc tgaattgatg aggggtttag ggaagggtat aaaagagttc aaggatggga
     8221 tgaatgaggg cgataagtca gaaaaaaaag agtgagctct aattttcttc atcaggataa
     8281 tatattagtg gctgtagttt ttttaatgct atttccaccg attcaacctt cgtaaatttt
     8341 aggctcagct tctgtttgct ttccctcata ctgcatgttt ttgggttgtt tttcaaaaaa
     8401 tctataacat gtgtaaaagc cattgagtta taatatgcag aatcctctgt tccagtaaaa
     8461 tacccagtca tatcaccacc tcgaagcaca attttttcaa agcctatcct actggcaatc
     8521 caccgcagtt taatagcgct aaacaaaccc tctacctgtt cggggatggg gccaaagcgg
     8581 tcaataagtt gatttttaat tttgtgtaaa ccatcttcag tttttatatt atccaattct
     8641 ttgtaaatgc ttagccgttc tgttgttatt gagatgtaag aatcgggtat cataagttca
     8701 aagtcagttt caagcaagca atccttaaca taacttttgt ttattaactt ttcggaatca
     8761 ttggcaaatg tctcctcttc aagttcttcc atagcttcgt cgaggatttt attgtacatc
     8821 tcaaagccta tttcagagat gaacccgctt tgttcggcgc caagaatatt tcctgctccg
     8881 cgtatgtcga gatcgcgcat tgcaacgcta aatccgctac cgaggctcga aaactcttca
     8941 atagctttaa ggcgtttacg agctttctgg ttaagggttg aaagtggagg tgaaagtagg
     9001 tagcagaagg cttgcctgtt agctctgcca acacgacctt tcaactgata tagatcgcta
     9061 agaccataat tttgagcatt gttgatgata atggtatttg cgtttgggat atccaatccg
     9121 ttttcaacaa tggtagtggc taccagtaca tcgtaatcgc cctcaataaa gtcgagcatc
     9181 acttgttcaa gcttagcgcc ttgcaattgt ccatgaccaa taccaacttt tactctcgga
     9241 caaaatattc tgaccatttc ggcaacttcg ttgatgtttt gtactttatt atgaacgaaa
     9301 aatacttgtc cgcctcttgc tacttcaaag tttattgcgc tttttataac ctcactgcta
     9361 aagctgtgta attcggttat tacggggatg cggtttgccg gtggagtatt gatcacagat
     9421 aggtctctgg cgcccatcaa cgaaaactgt aatgttcgtg gaataggtgt ggcagttagg
     9481 gtgagtgtgt caacatttac ttttaacttt ttaagcttct ctttgattga cacgccaaac
     9541 ttttgctctt catctatgat aagaaggcca agatctttaa attttacatc attacttaca
     9601 agcctatgcg ttccgatcag gatatcaatc ctacctgctt ccaactcttg gataatttgc
     9661 ttttgctctc ttgtagattt aaaacggttg atgtactcaa catttaccgg gaagtcgttt
     9721 agtctgtttg aaaatgtttt gaagtgctgc agcgccaata ttgccgttgg cactaataca
     9781 gccacttgtt tgctatcggt aaccgcttta aaagcagctc gtattgctac ttctgttttt
     9841 ccaaatccta catctccaca tataagtcta tccattggaa aatttttctc aagatcagcc
     9901 ttaacatcta ttgttgcttt taattggtca ggagtgtcct cgtaaatgaa cgaagcctcc
     9961 aactcgtgct gcaggtagct gtcgggagaa aaggaaaaac cgtttgatgc tcgtcgtgcc
    10021 gaataaagtt tgatcaggtc tcgggcaata tctttaacct ttttcttggt tctttctttt
    10081 aatctgttcc agttgttggt tcctaattta ttaagacttg gagctatacc ttcttttccg
    10141 ataaacttag ctatacggtg tagggagtga atgcttaaat agagtaaatc gccgtcctga
    10201 tataccaatc ttatcgcctc ttgttgtctg ccgttatttt cgatagtttc taatccgtcg
    10261 aagcggccaa cgccatgatc aatatgtgta acaaaatcgc cgggttggag gttgtaaagc
    10321 tcctttacgg atagagcttc acgtccgtat gtagccttgc gaatatgaaa aagatggtat
    10381 cggtcaaaaa tctgatgatc ggtaaagcag gcaactctgc aggtgaaatc ggtaaagccc
    10441 tcatggatag gtcgagctat ggtataatat tgtgtatgcc ttgatgtttc attttttgat
    10501 tggatgtcgt ccaaaatagt atgtaaacgt tcagcttgtt ttagatctac agtaaaaatg
    10561 aagttatcta tattgttaac tgaattttcg ttcagctttg tgataagcat atcaaaattt
    10621 ttgttgaacg aaagttgcgg ttgggcttga aattcaattg ttttatcagg ttgcatcagg
    10681 caacgggctc caaactcaat gacatggaaa tcgtttagat tagctaaaag ctcttgtcct
    10741 ccaaaacata tctccgaaac tcttccctta ttatcgttga ataaatatgt ttcatttagt
    10801 tttttacatt ccgattctat tttttcgaca gtataattaa gattttcaaa ccagaagatt
    10861 gagttttgat ctatatattt aataaatggg attttgctga ttttatctgt tgttttgtgt
    10921 agatcaggca ttattgttat ctcggacagg aaagcgaggg tcaattggtt agctgtatcg
    10981 aaggtacgca tggattctat ttcgttgcca aaaaactcaa ttctaaaagg gtgatcatcg
    11041 gaaaaagaga atacatctat gatgccgccc ctcacagcga attgaccagg ctctaccaca
    11101 aaattcactc tttcaaaatt catctctatt agaaaatctg tcatgaaatc aaagctaatc
    11161 tcatctcctt tttttacttt taatgaattt tggccggttg tgatgcggtc gggaactttt
    11221 tcggccaaag cctccgggta ggttattatt gccagaggat tccttgtgtc aattattttg
    11281 tttaacgttt cactgcgtaa tagtatgtta gagctatcag gttcatccaa tttaaaaggt
    11341 cgtttgtaag atgtaggcaa aaacaaaatt ttcctttcag catatttttt ttctttttct
    11401 tcgaaaatag tttcaagatc attatgaaaa taagctgcgc tctctttttc cggtaaaacg
    11461 aaaatatgag ttttgtttaa tagttttaat atcgctgccg cgacaaacga tggttgggaa
    11521 gctttaaggc cgctgatata caagcgtgta ggtaattcgt tgatgagtga ggcgtgaatc
    11581 gctgctattt ccgcgcttct ctgataacgg gtgattatct cttctactgt cacaacaatc
    11641 ttttttttga atgatctgca aaggtacttg agttgtgaaa tatgaactaa tagttcgaaa
    11701 catttagaat ttctgaaaca ttacctgtac ctttgccata caagtttgaa aaatataaac
    11761 tggtcagatc aactaattta tggtcaatat attagaagta aatcatgaag tggttttgca
    11821 tcaggtaagt tattcggatg cgccggcgct ctttcggctg attgacaata atcgtacaag
    11881 cttgcgcgcc tggttgccat ttgttgattt tacgaaagtt gttgaagata ctgaggcgtt
    11941 tattgggagt ctttttgttc cggggtcgcg caatttggtt tttaccattc gttacagagg
    12001 cgaagttgcg gggctgattg gatacaaaga tattgacaga aataataaga aattagaaat
    12061 tggctattgg atagctccgg tttttgaagg taatggtatt gttacacaat ctcttgaagt
    12121 tttaattggg gctgcattta gttatttaga gatgaatcgg attcaaataa agtgcgccgt
    12181 aggaaaccaa cgtagcatta atatacctaa gcggttagct tttcgcttcg agggaataga
    12241 gcgcgccggc gaatggctta atgacagatt tgttgacctc gaggtttata gtatgctaag
    12301 gagtgattgg ataaaatagt cgttattatt ttgtgtgggc ttttagctta acgcttaata
    12361 ttctaagagt ctgttaacca ttagcttagc tacctgtttt gatgcgccct cgcttcccaa
    12421 aactttatga agttgagaat agtcgtttaa aattcgctgc cggatttcgg gattaagtat
    12481 gtttttaagt tctgtatgga ggttactgct gttcatttct ttttggatca attcagtaac
    12541 gacaggcttg ttcaagatca gattcacgag cgagatgtac ttggttttta ttactctttt
    12601 agcaatttga tatgagaacc aattaccttt ataacatact atttgcggaa cgttcatcaa
    12661 agctgtttcg agcgtagcag tgccggatgt aactatggcc gcttcggcat gacgtaaaag
    12721 ttgatgagtt tgttggtaaa tgacttttac tgccttttcg tttaaagacc ttttataaaa
    12781 atcattttca acagcattag tgccggcaat tacaaactgg tattcaggaa aatgatcggg
    12841 aatagttaac attgtgggga gcatttttgc gatttcctgt tttctgcttc cgggtagcaa
    12901 tgctattatc ggtttgttct caagattatt tttgtcttta aatgagataa gatcatggta
    12961 atttgctttt tcattttcaa ttacatcgag caatggatgc cctacataat ctacttctat
    13021 gttgtgtttt aagtaaaagt ctttttcgaa cggcaggatc acatacatcc tgtttacata
    13081 ttttttgatt ttttttaccc tcgattgtct ccatgcccat atttgtggtg atatatagta
    13141 aaaaactctt actccatgtt tgcgggcaaa ctttgcaatt ctaaggttaa agcccggata
    13201 atctattaag attaaaacat ccggtttaaa acggatgata tcctgtttgc agaatttaat
    13261 attttgtaaa atggttttta ggttgacaat tacttcataa aaccccatga atgctagttc
    13321 gtgatagtgt tttaccaact ccacaccctg gtcatacatt ctatctccac cccatgccct
    13381 taggtgtgcc tgtttgtcca aaaatttaat ctcttttata agatttgctg catggatatc
    13441 gcccgatgct tcgccggcaa tgatgtaata cttcatctat ttataaaata tgaaatagga
    13501 gatgatgaga ataaatgtaa tggcaagtac ggcaaatccg gttttatccg attttttgac
    13561 aacaaaataa tatcttatcc agatgaggtt accggctgtt gcaaggagtt tcagcttgtt
    13621 aggcgaattg atggtaaaat ggaaatgaat tatcaattca actattccta aaataaaaac
    13681 atataacaca accggcgtgc atatccctaa aataatacca aaaagatatg agtctttatt
    13741 taagaaatgc cagaattgtt tcaccttact aatttatttg ttatacgtag tggctgattt
    13801 cgtttccaac aaagttttgt tgttatgccc aaaatcaaag ttgtactcac ttaggagctt
    13861 tatagattca tggttggtca ggtcgaattt catcggcact accgaaaccc aatttttttc
    13921 cattgcccat gtgtcagtac cttctttatg gtcggcattg ctaaatattc ctgttatcca
    13981 gtaataatta cctccatacg ggtcggttcg ttcatcgaat gcctctttcc atgaaccgag
    14041 tgattgcctg cacactttaa ctcccttaat aggttcatcc tgaagtttag ggaaattcac
    14101 attcaagcaa gtgttaaagg gaaggccttt acgaagaact tcactactta ttttttcgac
    14161 atacttttca atatgtgaga agtttgcgtt ggagtcgtag tcgtcgagtg agaagcctat
    14221 agctggaatt ttgtcaatag cagcttcgat tacggcagcc attgttccgg aataaaatac
    14281 gttaacagag gcgttggagc cgtggttgat gccagaaaca accaggtcgg gctgacggcg
    14341 caaaatgatt tgaacgccaa attttacaca gtcaacaggt gttccattac aactatattc
    14401 actataaccc tcttcttggt gaatttgggt aactcttagg ggcatagtga ctgtaattgc
    14461 atgcccttgg ccggaacgtg gcctgtcggg tgcaactaca actacatcgc caagatttcg
    14521 cattattttg atcagggtgc gtagcccgta cgcatttatc ccgtcatcgt tggtaactaa
    14581 aatagtaggt tttgtattag ccattttaaa actttaatca aacttttgta actcctgcag
    14641 agataatata tttcatcaca tcggtggctg aggcattaat ccgtgttaca ttttttgccg
    14701 gaacaatgaa tagattaccc gaccatgcat aagagtgagg caggtaaaca gctatctttt
    14761 cttccgagac acctattgag ctcaaatctt catcagtaac aaatccaaat ttttctattg
    14821 taccctcagc atttactttt acaagtaccg gctggttgaa acgctttttt tgcccaacaa
    14881 atgctgcaat aaggtctttt accgaggtgt agataatctt gacaagcggc gttttggaga
    14941 gaagcccatc aaaatagatt gccaatggct taaacagaaa tgaggaacca agaaaaccaa
    15001 caagggtgat aaccgccaaa acgagtatta atccaaggcc aggtattggc cggccaataa
    15061 agttttcgat atacttgtat agaatgccat caatccagtt aaagaccaac catatcgaat
    15121 aaatggtgat tgctatgggg ccggtaaaga gcaggccttg caaaaaaaat cgtatcaaac
    15181 gtgaaatgat tgttttggat ttcattagga ttttacaata attattaatt caatatttta
    15241 aacaacggaa aggactgaat gttattgggt atttgaaaat aaagcatgtt cggagtagag
    15301 gtgtattatt agatggtttg aggtggttga aaaagtattt tattttataa tggctcatta
    15361 aaagaacttt aaaaagactt caactctctc catagtcgtg ctgtaggtag ccccataaca
    15421 ttgaaatatg agccttttat actttttatc cctatcatcc cgatccactc ctgaatacca
    15481 taagcgcctg ccttgtcgaa aggtttaaac cggtcagtgt aatattcgat ctctttaagg
    15541 gtgagttttt cgaaaaccac ttccgttgtt tcggtgaagg taatcatttt ttcttttgta
    15601 cgtaacgcta cgccagtgat aactaaatga gtcttgcctg atagttctcc gagaatttct
    15661 atcgtatgct cccgcgattt aggcttgctc aggattctat tatcaaggca aacaatggta
    15721 tcagcggcaa taatcaagtt gtctttaccg atatcttcaa ggttgaatgc gcacgctttt
    15781 ttactgcaaa gatacagtgc aacctcttca ggcgtcaggc tcgtatcaaa attttcatcc
    15841 accggtttta ttctgatttc aaaatccagc cccatacttt cgatcaattg ctggcgtctt
    15901 ggagagttgg atgcaaggat aatatgaaat ttagttttta gcatagcaca ggaagaattt
    15961 tttattgaac ctataataat ctttttaccg ctcttttgtg gttggttccc gatttactaa
    16021 atactttttg gatttttttt gttaacaatc agtgctttgg agtataatat taacgattaa
    16081 gcatgttgat gactttttca agatcgccat cattaggcgc tggttctaat ccaagtattt
    16141 cagccattaa tacgtagagg ttcacattgt cgaaagccgg atgaagatag ccttttttaa
    16201 acgaagggcc aacggcataa aagactgcgt gcatttcggg gtaatcggga tcgtaaccgt
    16261 gagtgccttg cgcatataaa taattgttgg attttctcca gttgattgtc caccctaagt
    16321 cagcctccac tgtaatatcg agggttcttg gattagttcc atagtggtat ctttccaaca
    16381 tgtcgctatt tttccaggat tttacatgtt ctgttttttg gagacgattg aatactgagt
    16441 caagatatcc ttgtttagcg ccgacattga ataccgggtt ttctcccatg gtagtttcta
    16501 accactcatc agggatgatt tctctcatat taataactct atcttctgag agttgtgcca
    16561 taccatgatc ggaggtaaaa atgagatcaa ttttctttgc ataaggtagc atgttgatct
    16621 tacggcaaag gtctccaatt aatttgtcaa tatattcgac cattaatata gtttcgttgc
    16681 tttcaggccc aaatttatgt ccaatggcat cgggttcgtg aaagtagcac attataagtt
    16741 gggggcggtt ttttttggga agtttaagcc aatagataag tgtgtcaatc cgatcttcaa
    16801 atgagacact ctcgtcatat tttttccagt aatcgggttg gatatctttt atcggtagtt
    16861 cgctacccac ccaatagaat gatgcagttt ttaatcctgc ttttgatgcc gttacccaaa
    16921 ttggttctcc gccataaaaa tatgggttta aacgcgctgc gctgtctttc attttatatg
    16981 ccaacccagt tgcagggtcc ataaagctgt tgtttacaat cccgtgatgg tcgggataaa
    17041 ggccagtggc catggtgtaa tggttaggga atgtctttga tggaaaagcc ggaagtatgc
    17101 cttctgccct tacgcctatc agcgcgatcg aatcaaggtt tggagtattc gatttttctg
    17161 tgtaatcttt cctgaagcca tccattgata gcacaataag atatcttgcg tcattggtcg
    17221 tctttgattg tttgcaagag gtttggagta ataatagggc tgcgaggaag aaaataactt
    17281 tatttgtcat aactatgttt ttggtaaaaa taaactaaaa catactactt tttaaaaaac
    17341 tgaattttaa tttttcaaat aaaagacgat tcgatttttg atttaattag attgtaagct
    17401 attgatcggg atttgtatgc tgttttatta aaagaaaaag agattgtctg aaaagccgga
    17461 caatctcttt tttttattaa accaaaacga ttagaagata tatcttatcc caaactgaag
    17521 ttgccatgcc tgggtatagt tataattgta gtcataggtt tctgttggat attcaccttt
    17581 aacttttacg aatgagaatt cggggacatt gttagcgtct tttccttcat attttaggat
    17641 acgtccgtta ttggaaactg tattcgattt ataaacgccc catttgctgt ttatcaggtt
    17701 gccgaagttc aggcagtcga ggctaaactg cagggtgttt ctttttccgg caacattaaa
    17761 gtagtactcg cgtgcaaggc gtaggtcgaa gcggttgacc catggagcac gggcagcaaa
    17821 ggcctgggca tattcgccct tgtgtttgct tagatatttg tcctgatcca taaatttgaa
    17881 gaatgcatct tcatcttcag gagtaataaa tttaatgtcg cctttatttt tcggtatgta
    17941 aatcaaatcg tttcctatgc cgtcaccatt catgtcgttg gtgtaagcaa atgtattacc
    18001 ataaggtgta aatccggtgt agaataggtt aactgttgta gctgatttca aggagttgtt
    18061 tccccatggt attttccaga aagctgatcc gattagtttt gatggaacaa cgtactgtga
    18121 acgctgtaat atggggagat gtggcccgtc aatttccatt aagcctgtat aggcagatgc
    18181 ggcgttactg cctggcatcc ctgaaatctc ttttgactca gtataagtgt aggccaacat
    18241 gaggcttaag tcttttaccg gttctgcgaa gattgagata ttaccaattg cgccccatcc
    18301 ttcgtttgta tttgtcagaa cgtatgaatt ctttttattg taggctaaat cagcataggc
    18361 tggatagatg tacctgtcgt ccggtccgct gaaacgttgc catgtgtcgt caggttcttt
    18421 taagtcccag ttttggagca ttacaccatt gattgttttt gtgaaaatac cttcaatagt
    18481 taccgacaat gggaaagagg ctggtaggtc gtaatccaga gctaatgaag ttttccaaac
    18541 ctgtggcatt ttaaagtcag gatcaacgct atttacatcg cgaggaagta ctccctgttc
    18601 tggggtgatc tcatttggta atcctaaaat gctaatcatt tcatttacat cggtaatcat
    18661 tttacctgca agcatagcta gtttggggtc ggcgctgata acgtttccat tagcgtcaaa
    18721 tttggttaca gcagcgtaac taccctgtac catgccggag ttggtaggca tattggtgaa
    18781 aaatactaac ggtagtcggc cagcaaataa tccggttccg ccacgcagtt tcattttttg
    18841 atcacccttt acgtcccatg taaaacctac tcttggtgat atttgtagtt ttgcgtcagg
    18901 ccatttgccg gtatcaattt ttttgcctcc aaaatctaat tcactgattg cgttgttgct
    18961 catgatgtta tcttcataag caagaaaatc gccacgtacg ccataggtta atgtaaagtc
    19021 aggattaaca ttccattcat cctgagcgta gatgccaatt tgattgaaag ctacttcagc
    19081 ggcaggattt ttctcaccgc catagccata agtaacggca aagtcggttg gagtagcttg
    19141 attaagaaaa tcgtcaaggc ttgaatagcg gtaataaccg gttccgttac gcatgtatga
    19201 gttgtttgcc atctgatgct caaagctggc tcctatagtt accttatggt tgttcaaata
    19261 gacgtttacg ttgtcaataa tattaaagat attgttattg actccgttgt tccaggtaaa
    19321 taattcgtaa ccggcagaaa tatagggttc aagaatttgg gtgccgtcat cattacgccc
    19381 atacataata tcaataaatg ggaatggaga agaattcgat cccctcatat cttttatctt
    19441 ggtgtatgtg gtgaggaatt ggtttgaaac tttatttgaa aaacggctgt ttaagtcgag
    19501 ggcgagggag tttacagtat tttcctgcga ataaattgag ttggagaaag ccattgaata
    19561 ctgcgaaata cggtttaatc cgcttaagcg ataaccggcg tcggttgagt tgccgtttgt
    19621 ttggtaccaa gcttgatttt tagtaaagtt atacctgagg ctaagtttgt ttgagttgtt
    19681 aatattccag tcaatacgag ccataagctt tttgttactc tcgtctgcgg ggtaatcggt
    19741 gtatgagcct ggatcgtagc cataatttgt aagcaagtgt tgtttcactt tttccatatc
    19801 tgatttgctg gtacgcgaca gcattaggtc agtgtttgca acgccatctt ccgaaggtct
    19861 ccaattaacg acttgtccgg gttttaactc atattcacca tttacgaaaa agaaaagttt
    19921 atttttaatt ataggtcccc ccaacgagat tccgtaagtt tttgtggatt cgatatttct
    19981 aattccaaaa tcaatctcat caatcctatt acctctcatc tcctgatttt tgtaataagt
    20041 gtatgcagta cctttgaatt cattggttcc cgattttgta atcgcattga ttccacctcc
    20101 tataaagttt gtttgacgca catcaaaagg ggcgactacc acttgaactt cttcaatagc
    20161 atctaacgaa attggatttc cacctccagg taagttggag ctaagtccga agttgttgtt
    20221 aaagttggct ccatctacag taaagtttgt agatctacca tcgctgccgg cgaaactcat
    20281 gccattggca aaaggtgaaa gccgggcaat gtcttgtacg ctgcgagtga tggttggcat
    20341 tgagttcatt tgttgatttg agatattggt ggttgcgccg gttctctctg ttgtaaatct
    20401 tgttttttct gccgaaacca caaattcctt caattcaatc atctcctctt gcatctttac
    20461 attaagaaca taagtctctc caaggctaag aataatatcg tctatgatat tttttccgta
    20521 acctacaaac gaaatttcaa ctttgtacgg gcctccaaca cgcataccgt taaggataaa
    20581 acgcccgtcc acgttggtaa tggttccata cgttgtgccg gaagggatgt gtgtggcaac
    20641 aattgttgcc ccaacaatag taccctgcgt gtcagtaaca cgtccagtca tactggaggt
    20701 cgttatctga gcatgtaacg ctgtagtgaa cagcaatagc aatgaaactg ccagaaagtt
    20761 taattttttc gacataaata atttgttaag ggtgaataaa tataatcttt aagtaaataa
    20821 ttggtttcaa agaaaaggaa tttttaccat cgctaaaatt ctttttatta aaacttttca
    20881 acaaaagtta atatggataa gcaacaaata gattttgagc tgaataacat gtaaatatgc
    20941 tttaacaaat aaaatttaag agaacaaatt tattgaacat aacattttgc cctttacaaa
    21001 aggaaaaaag atatttttgc agcggttaat attcaaatta ttatgcacga cgagcgtgat
    21061 tctattgatg gtgaacttga tgaattaatt ggtcgttttg atgagatggt caggcacgac
    21121 aaattgtatt tttttgatgt tgacgagttt gaagaaataa ttgattttta ttttgaacat
    21181 cataacaacc ggcgtgtaca ggtggctatt gaccatgcgc tcaggcagca tccctcttca
    21241 catatctttt tgcttcgcaa agcacaatat ctggctgata tcaacaagtt gggaaaggcg
    21301 cttgaaatat tgcaaaaact tgaactgctc gaaccgcaaa atcctgacgt ctatcttacg
    21361 ttagggacgg tgtacagtaa attgaaaaag ataaagcaag caattgatgc ctattataag
    21421 gctattgaat actctgacga aaaagacgac atatatattc ggatagcctt cgaatacgaa
    21481 gagttgaacg attatgacaa ggcgatcaaa tacctgatta aggctttgga ctttaatcca
    21541 aataatcaat cagtgattta tgagttgtcg ttttgttttg aactcgccgg cagagttgaa
    21601 gacgctatag ccttttttaa tgagttcctt gataaaaacc catactcaga tttcggatgg
    21661 tttaatctcg gtattgcata ctccaccatt gggctgtttg aaaaagctgt ggatgcttat
    21721 gactttgtaa ttgctatcac acccgatttt tcatcggcat atttcaataa agccaacgca
    21781 ttagttaatc tcgataaata tgctgaagct gtggagactt acaaagaggt cttgaagtat
    21841 gaagaacctg aagcggttac atatttttat ataggcgaat gctacgaaaa acttgaacac
    21901 tattcggaag cctttgatta ctaccggcgt tcgtcgaaac tcaatcctga atatgctgaa
    21961 gcatggatgg gaatgggttc gatacatgat aaaatgggta atgagtcagc agctatcagg
    22021 tattataaaa aagcagtgga gcttgacgaa tatgataccg aaaactggct tacattaggc
    22081 gatgcatact atcgttacga taaatttgat aaagcaaagg atgcttttcg taaagcctgt
    22141 gaagttgaac ctttgcatcc tgatgtttgg cttgattatt ctgctatgtt tgctgaagag
    22201 gaggattttt ccaatgcaat cactatcatg atcaaaggaa tagaacatca gcctcaaaat
    22261 gcagaatttt attataggct tgccggatat ctgatgatga aaggaagaga aaaagaggca
    22321 attctgaatt ttgaaatagc tgttcaaata aattatgacg attattatct cctgtttgac
    22381 ttctatccgg agttggctga taacgcgaga tttaatgaac tccttaatat ttacaaacct
    22441 ttgaaataat ttggataatt gtccatttct gagtttaaaa aaaatacctg atgcaaaaat
    22501 atttttactt tgtcctgaag ggattaggaa tgggagctgc taacgtaata cctggtgttt
    22561 ccggcggtac aatagcattg ataaccggaa tttttgagcg gttaataaat gctataaaat
    22621 catttgatat tactgcacta aaactttttt tcaccggacg cttcaaagat tttgcccgtc
    22681 atgttgatct tgcctttctt gtagctgttt tctttggtgt tgtgctggca ataattagtg
    22741 tcgcaagact tttcgaatat ctttttgcta actatcccat atatatctgg tcatttttct
    22801 ttgggttaat tgtagcgtct gtctattttg tagggcgtac tattagtaaa tggagcctga
    22861 cggtaataat agtttttgtt gcaggaactt cgttggcagt attgcttaca tttttaaagc
    22921 cagccgctga aaacgataat ttgctctacc tttttatttg tggcattgta ggagcctgta
    22981 gcatgatcct gccaggcatt tcaggatctt ttgttcttat actaatgggg aattaccagt
    23041 tggttatgat taatgcggta aaccagatga acgttagcat ccttgcacca gttgtgcttg
    23101 gcgcaatttt cggccttata gcattttcaa actttttgtc atgggttttt aaaaaatttc
    23161 ctgattatac tatagcatca cttacaggat ttatcttagg atcattagga attatctggc
    23221 catggaaaaa taacgtttac cagatagatg ttaatggcgc ttttgccctt aaccgaaaag
    23281 gagaaaaaat agttacgggc tacgaatggt ttttacctga tagttttaca actgaagtgg
    23341 taattgcctt gggattcatt gttctggggt tcttaacaat ttggattatc gaatatttgg
    23401 ccggcaagag ggtaaataaa tgaaacgttt cggcattatt ggaaaaaaac tgtcccattc
    23461 gttctcagca gattatttta acaaaaaatt tgaaaaagag ggatttggcg attgttcgta
    23521 tgagatgttt gagcttaatg agcctaatga aatatccaag cttgttgaaa ccaataacga
    23581 ccttattgga gtaaatgtaa ctattccatt taaaactaat attatccctt ttttagatgc
    23641 attggatccg gttgccaaac aggttggcgc tgtaaataca atcaccgtaa accgaatagg
    23701 agatagggta aaattagttg gatacaacac cgatatttat ggtttcgaac tctctaccaa
    23761 cgcttttgaa acctataaaa atgctgttat acttggtaca ggtggagcag ctaaggctgt
    23821 tagccatgtt ttgaagaaaa aaaacattaa ccatttgttt gtttcgagaa aacctacttc
    23881 ggaaaatgaa ataggatata aagatattga tgaaaacgtg ttgaggcgct ttctctggat
    23941 cattaacgcc acacctttag gcatgtttcc cgacattgac agcttcccgc caatacctta
    24001 tcatcactta acctcccgac attttcttta cgatttgatt tataatcctt cggatactct
    24061 ttttttgaaa aagggctttg aagctggggc aaaaactaag aatgggcttg atatgctaca
    24121 ccttcaagct gaaattacat gggttatttg gaaaaagatg atttgaagat ttgttctctt
    24181 aaaattatgt gtatcaaaat gttaatcaat aaactttgaa cttgccgtaa aaaatatttt
    24241 ggataatgtt attttgaata aaaaattaat gactactttt gcagccagtt ttaaaaaaaa
    24301 ctcgaataat agatttagca atgagtaaga atcaaatatt aagctacgtt gaaggtctta
    24361 caccggttac tgtaatgcct gactttaagg caggggatac cataacggtt aaatataaaa
    24421 tcagagaagg taataaagaa cgtattcaga attttgccgg tgtagtgtta cagcgcaaag
    24481 gtcaaggacc aacagccact tttaccgttc gtaagatatc gaataatatt ggggttgagc
    24541 gtatttttcc agtgtcgtcg ccctttattg aagaaattgt tgtgaataaa cgaggcgttg
    24601 ttcgtagggc acgaatattc tatctaagag gtttgcgcgg taagaaagca agaatcagag
    24661 aaaagagagt ttaagataaa taatcaaagt gtttttagtt tttttagcct ggtgtgtttt
    24721 gacatcaggc tttttttctt ttagtcaatc cttaaaagca tatttaaatc ctgtttataa
    24781 aaaataaact gttgataaaa aattgattct gagtgttccg cattgcaaag ttgg
//
LOCUS       DAOOAI010000020        24273 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_2713_length_24273_cov_5.689322, whole genome shotgun sequence.
ACCESSION   DAOOAI010000020 DAOOAI010000000
VERSION     DAOOAI010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24273)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24273)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24273
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2713_length_24273_cov_5.689322"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1515)
                     /locus_tag="PK345_02655"
     CDS             complement(<1..1515)
                     /locus_tag="PK345_02655"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPX33953.1"
                     /translation="MRRFTLLFALMIFIGVQWAQAQTRTLSGSVTNADDGSTIPGVSV
                     VVKGTTVGTTTDLDGRYSLLVPADAKILQFSFVGMKTIEILIGAENVINVTMQPEVTA
                     IEGVVVTALGISREKKSLGYAVQSVADEDLIRTGNVNLLGALQGKVSGVDIKPSSGMP
                     GASSQLQIRGARSFTGNNTPLYVVDGMPISSTPAYSTGNSVTGSDISNRAIDINPADI
                     ESIEVLKGQAAAALYGIRASNGVVIITTKSGKYNQIGKPVVSIGHNSSFDVVSRTPDY
                     QTTYAQGSGGKYVNNSSYSWGPKIVDLPDDPTYGGNNHGHPGMYRVMQLEQAGLDPWV
                     YPKVYNNWNDYFRTGYTMTNNFSISQAVKGGNFSLGYSNTDQTGIALNTGLTRHNVKA
                     NAERILNNYFKVGFSSNYSNVGIDKLTGANDGSLAGVLAAPSSYNLAGIPYHRPGEPT
                     RQIYYRGGSFDNPYWVEHNNTFNEKTGRFFGNAYIDFNTNVSEGMDLKVRYQAGV"
     gene            complement(1734..2333)
                     /locus_tag="PK345_02660"
     CDS             complement(1734..2333)
                     /locus_tag="PK345_02660"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPX33954.1"
                     /translation="MKHDFYLLLIPIFLFVPTLLCAQEFNGGLIAGLSASEISADRIE
                     RPNKAGIYIGGFVNRYITPKSSLQMELNFIQKGSRMNPDSANNFSSYLLRINYVELFL
                     HYKWDFHHLFTLEAGPSLGVLVSQHEEADYQLLDNPFHLFDLSMNIGLFYNINQRWRF
                     NLRYSNSVIPVRPHSGGQTYRLNRGQYNEVLSFTFHYLL"
     gene            2626..5859
                     /locus_tag="PK345_02665"
     CDS             2626..5859
                     /locus_tag="PK345_02665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005862535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPX33955.1"
                     /translation="MQKISTHPILEVPLREKTTFTFNGQEIEGEKGFTIAAALHQAGF
                     PVHSHSLQDRNRSLECGIGKCGACEMLVDGKVKRICITKVDGIKEVTEIPKEYTPTIE
                     YINETEPVKVYKTTVAIVGAGPAGLACREILNQHNIFNIVIDNNDKIGGQFLMQTHQF
                     FFFEKEKKFGGMRGFDIANTLAGNNHTGIFLNSTVWDILEGKRLAVKNILTDDVYYVD
                     SEYLVVATGAVPFLPAFKNDDLPGVFTAAVMQKMMNNEFTLLGKNILTVGAGNIGYLT
                     SYQLMQAGGCVKAIIEAQPNEGGFPVQANRIRRLGIPIMTSKILLKAIPNKENTGITG
                     AVIADCKNFEPIPGTEKLIDGIDAINICTGLESDDQLLIKGREIFGRNVYGSGDAIRI
                     GEGTSAVLKGKQVAFEILDDLAEHFDYNEYLAISKEYIDSQQHPVRVIERAFDPTEER
                     IWQKPFVIIDCLYGFACNPCEFSCPQGAIRKTSTSTVPTIDFEKCIGCMECVYQCPGL
                     AIFGYNINKDWIFLPIEYDVDEGSDVFLVDNNGSVLGEGVLEKILKKPNKTNVARVKS
                     KDIHGRDLLNVRGFIVKSNYPEPLNLQPFTNEIQTEQYICHCDDVRLDEILEVIGNRK
                     FISVDEVKHTTRLGMGACRGKRCIKRLKLVLKSSGISIIGDPTPRAPLSNQVSLGDIY
                     PKKVKEQIIIPLNSRKTSKIKVEALVAGGGIGGSALFRYLAEAGLKPVLANHGRGSSW
                     RNIAGGRPNFSLPELSDIASQNFEIFKELQTISNIDFRTIDYVTFAHDNQLLSALEAS
                     MAWSDAYIIEPKDFVKHISPHFNPNLKTYKAAMVTKNCWQATPGKVVDLIRQIGLKHG
                     GVICEDCEVIDIGKTGNTYTVLVRNHEKEYVEYQTTRFINAMGFQGDHFAKKIGIETG
                     VYPVKHQAFITRRLPMMGINGIPLPMIIDRRQYKGFIAVYGQQLGETGQIIGCASPHI
                     EPYETDKNLKINSKDFLEIVSEMFVDWIPELSSVGFQAVWAGYYVEPRMILDPENGLF
                     LGLRGQGFMLGQYLAKMYVDKIIGNPVPTYFDRLTLKGDGLLEKAFK"
     gene            5930..8653
                     /locus_tag="PK345_02670"
     CDS             5930..8653
                     /locus_tag="PK345_02670"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33956.1"
                     /translation="MNPFRTSILNTILLFVFLSTVIIALTIKFTKTNTTVDREAYEVF
                     LREKMDKIPLADATESKKIPKPAQPDRAAIQNYFMTLDPKTGVVPKERLMQVYNDLKS
                     NPKKNRINPLEWSGTSANMGGRTRAIMWDPNDPQGKKVWAGGVTGGLWYRNDITNNNN
                     EWQPVDDFWSSLNISCITYDPNDPMIFYVGTGEAETALIIYRESSGVGDGIWRSTDGG
                     QTWSLMESTIAFEYVTDLLVRDEGGQSVIYAGVASGNYKGSIHYSEPSDGLFRSEDNG
                     QTWQQVLPDIPEYGEPYAVSDIDMGADGRIYVGTMPNVDIEGGAVILYSDEGLSGSWW
                     VYDNYVQIIENNPQYKIPGRVMVAPAPSDPNIVYAQIAAGYNSYFNYYRGRYILKSTN
                     KGQTWSSVMIPESTWSTLAWHAFTLRVDPNNANNIFTGGLDMWKSNNGGSSWTRISDW
                     SLMYYGGGDQYLHADHHDIQFKPGSSTTFITCNDGGVFYTETATSSFPVFQEKNQGYN
                     TLQFYTGAIHPSAGSTIYAGGLQDNGSLLYNGQPLTINDMVSGGDGAACFFDDNDPLL
                     ITSVYYNRYYVFMNYNMYTYYDYESGIFINPADFDVKTNTLFANAVSFSGGNPNTILR
                     IINIPYNATASFTGLNTNTNVYYSHIKASPYAEAGKSTIFVGTQSGRVYKVINAEAVP
                     QTVEITGTNFPTAYVSCIAIGSSEDILLVTFSNYGVESLWQTIDGGETWQNVQGDLPD
                     IPVRWALYHPENDNQVMLATELGIWTTNTINSPEVIWKQDAQGMANVRVDMLTLRNSD
                     NTVLAATHGRGFYTTEFKLITGVAINKNNNETINIFPNPTNGLINIDFGSMDQKSINL
                     KLINVSGKVVLAKKLPEGKFHTLDLTGFPKGNYLISIFSTEKKISRKIVLQ"
     gene            complement(8663..9712)
                     /locus_tag="PK345_02675"
     CDS             complement(8663..9712)
                     /locus_tag="PK345_02675"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPX33957.1"
                     /translation="MQSEQNKVLKIFRFERVIIPVIIGLGVVGYFSFKDFDPKPFFEI
                     QPSFQLWFWLFMAFTMVVMRDVAYMYRIRLLTDKQLSWRNSFHVIMLWEFASSVTPSV
                     VGGSAVALFIVTKEGIKPGRATAIVMITALLDELFYIIMVPLILLFAGTSLLFKSESS
                     FLFINSKLGIQGVFVIGYFFIVFLTLIISYGVFFKPRGFKWILLRIFKIPFLRKWRFQ
                     AAQAGDDIIITSAEMRHKSFSFWARASLSTWISWTSRFLMVNFLIYAITAGGDHFLIF
                     ARQLVMWVIMLISPTPGSSGVAEFLFSDFLGDFIPAGLNMAVALLWRLMSYYPYIIIG
                     ALVLPIWIKRVYKFN"
     gene            10111..10533
                     /locus_tag="PK345_02680"
     CDS             10111..10533
                     /locus_tag="PK345_02680"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HPX33958.1"
                     /translation="MMRWNPDTPLSDLFENFFRSHSNERMERKHYDCSPSTNILEKDD
                     SFELHMAVPGVKKEDVKIDLEKNVLSISSEKKAEKTEEVEGKYSRREFLYGTFCRSFT
                     LPETINVDKIKADLKDGILKVILPKKEETRITKEIKIA"
     gene            10689..13046
                     /locus_tag="PK345_02685"
     CDS             10689..13046
                     /locus_tag="PK345_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013283.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BamA/TamA family outer membrane protein"
                     /protein_id="HPX33959.1"
                     /translation="MKGKFFLLPVLLLLISSCRITHELKDDEKILVRNVINIDETKIE
                     PKKLNFDPNDLYSILNQKPNKRFLGVFRFREYIHIKNYQKAKKPSFKYWLSKNFGKEP
                     VFYDELAAERSVSQLQLFLNNQGYFNSQIENEVSFKKHKAQAIYAIKIAQPYQINQIE
                     YQIPDTAISSIIINNKSKSLVKPGAIYNVNLLNDERYHITTLLRNHGYFYFTPDYVFF
                     EVDSAFNNHSLNIYTNISNPKIPIKDYPDSISESLHKKYFINRIAINTEFSPLRRDTT
                     QLMAYRDTTSKDNRNRYIYYYRDKLKVFPKALRNQISFKPDELYTEKDQENTYRQLSG
                     LPLYGYTNFSFTPTAVNDFSNNLAGYKLDALINLSRKPVHSFSVEAEGTTSGSTLGIA
                     GNVVYQNLNIFKGAELLTLKATGGLEWQQGGPETLDVFSFINTIQAGAEARIDFPKFL
                     IPFYSGQVSKSSRPRTSLMMGINYQKRPNYTRYITNASFGYNWKMGKYVYHSFVPVEL
                     NSVSIFPDSSFVRLLKDINDPRLTNQYTDHLILLSKYSFIFNNQERNLVKNFTYFRWG
                     IESAGNFIYILNLITEPKINNKGETILWNIPISQYIRTDIDIRRYLTLDEDNTIVIRG
                     LAGIGIPYKNSLVLPFEKGFYAGGANDMRGWKYRSLGPGAYRDTTKRYFEKMGDLILE
                     LNLEYRFPIYGFLKGAIFGDLGNIWLLTFSENYPGGLFKWKDLPNQIAFSSGAGLRFD
                     FSYFIFRVDLAAPMRAPGYPIGEQWRIKTLKPKDIIWNFGIGYPF"
     gene            13062..14018
                     /locus_tag="PK345_02690"
     CDS             13062..14018
                     /locus_tag="PK345_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009597674.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HPX33960.1"
                     /translation="MQYHWGHQRRFNSYAEYFKSHFGTRVQKVSVDAGFTCPNRDSLK
                     GTGGCTYCNNAAFNPSYCNPTKTIKQQIEEGIEFHRKRYRRADKFLVYFQAFSNTYAP
                     LNHLKTIYEQALQQDKVVGLVIGTRPDCIDDEKLQYFADLAKKNYIIIEYGLESCYDR
                     SLQNINRGHTFADSIEALTKTSKKGIKTGAHIIIGLPNETDDEIINQADILSKLPITT
                     LKLHQLQIAKNTIMEIQYRKNPNNFRFFEINEYIELVIAFLERLNPTIVLERFAGEMP
                     PRFIAGPHWGPIRYDVILQKIEKRLLEKNTWQGRLYQEIQSF"
     gene            14111..17440
                     /locus_tag="PK345_02695"
     CDS             14111..17440
                     /locus_tag="PK345_02695"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX33961.1"
                     /translation="MKKYLLSIIAMFLMGFIAVGQVTIYEDNFDSYTAGEKLTIENPE
                     NWTTWSNAPGGPEDPVISTDYSFTNPNSVKITTDNDVVLLLGGKTVGKYNLGFKMYIP
                     AGNLGYFNVLQDFAGSGSTWGLQVYFDLGGAGLIDGDGAGAASFVFGYDQWLNLKVFV
                     DLDNDWCKVFLNNSQIHEYQWSKGTFGTGMLKLDGMDFYGWDDGGTPLFYVDDVYYEM
                     VTESLIVEDFESYNAGQHLVQQAIAQGKDYWTTWNNSPGSAEDPMVSSDHSVSGSKSV
                     LIEGTNDVVLLFNDLVTGAYAIEFKVYIPQGFYGYFNLLQDFNGPNSEWGMQTFFDQG
                     GLGTVDAGGQGAGVFTYAYDTWIPVKIDVDLNNDFAELFVNGNSVVTWVWSGGTFGQG
                     ALNKLDAFSMYAWNENGTPKAYFDDISLTQTAPPGGDPEINVSPTSFTETLQNGQTSQ
                     KTLTISNTGVVKLNYNIIVNFDVDDGDAETEYTQEVVIEPETTITGNDIAVIKAEKSQ
                     PGPSVTDDEVLLHYDGDYSSAVGLTNGGTFEIAARFPAALTGQYIGMELTQVRVYIND
                     PANGYKIKIYGQSHPDKPGELLYEQLFSANAGAWNEVNLYTPLLNTGGDMWVAYEIVQ
                     NVAGVYPAGTDAGPAHADGDWIKTGSTWAKLSVINPNINQNWNIRAKLVGDPMQAWLS
                     VDPSFGSIEPNMSESITVNFNAGSLPEGTYQANINIASNDPVTPLVSVPVTLNVQGGG
                     CPLPPPTNLTLQQTGTNPYNVVLNWVAPEEPGGTIRWDDGINYDGIGLTNGGTFSVAA
                     KWEPGQLTEYIGLYLKQVDLFPRSATATYVVKVWTGTAGNQQLVSQTATLISEQWNTV
                     TITNPVKINAGMYLYIGYETTHGAGEFPAGADAGPAVANFGDLISTGGAWASMSIQFG
                     LNYNWNIAGIIGVGSDNATPAEPIVITPKPGASYGLPIAGNLGKTNNAQWVNNSRALE
                     GYDIMRDGQIIGSVGSTVTTYTDNNVPLGAHTYKVGAHYTECTAWSDPASIGVSISEP
                     TENINTLSIYPNPANGEVNISSPTDMKQLKIINYTGQVVYQSQPNNNHIIIKIADLSA
                     GMYLIQIETTEGWTTSKLVIK"
     gene            17508..17846
                     /locus_tag="PK345_02700"
     CDS             17508..17846
                     /locus_tag="PK345_02700"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HPX33962.1"
                     /translation="MNDDLTFFDKVYLVVKQIPFGRVTSYGAIAAFLGTKGSARMVGW
                     AMNASHNYIENIPAHRVVNRNGMLTGKNHFGSPHLMQQLLESEGIKIENDKVENFKEL
                     FWDPAVHLKI"
     gene            complement(18055..20286)
                     /gene="selD"
                     /locus_tag="PK345_02705"
     CDS             complement(18055..20286)
                     /gene="selD"
                     /locus_tag="PK345_02705"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: protein motif:HMM:NF002806.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HPX33963.1"
                     /translation="MKLEYNIYLDYNATTPIGDEVAEAMRPYLKDYFGNPSSSHSFGA
                     AARNAVENARRQLSELLNCHTDEIIFTSGGTEANNYAIKGVAFQYRNKGNHIITTTIE
                     HPAVSEVCRFLEANGFKVSYIPVDDFGIVDPQNIEKAILPSTILVTVMHANNETGVIQ
                     PIEKIAEITKKHGVLFHSDAAQSIGKIETDIQKLGVDLLSVAGHKFYAPKGVGALFIS
                     RGVKLQKLIHGANHEQNLRAGTENVLEIVGLGKAAEVAKRDFQTNIKQMMLTRDKLAS
                     LLFTALPNIRLNGHPDLRLPNTLSISFPNIEANTLLSELKEIAASAGAACHADQIDVS
                     KVLTAMRVPVDFAMGTIRFSTGKNSAIEDMEKAAELIIAAVKRLSPDEVIQDKTKMET
                     NEIKLTDFTHGLGCACKIRPQYLEQILKDLPLSIDENVIVGTGTSDDAAVYRINKSTA
                     IVQTVDFFTPIVDDPYYFGAIAAANSLSDIYAMGAKPLFALNIVGFPDNRLPIYVLKE
                     ILKGASDKAAEAGISILGGHTVEDIEPKFGMAITGMVHPDKVLTNATAKPGDVLILTK
                     PIGTGIISTAVKRGIVKKNISLIARNVMLELNADAANLMSLFPVNACTDITGFGLLGH
                     LKEMMEASGVGSIIYSSKVPIIEGVYELATAGAIPGGTRNNLDYVSANVKWDENIPEI
                     TKLILCDAQTSGGLLIAIPEAFGAGFIRKLNENHNKQAEIIGKIIETDETSTGIIQVI
                     NLK"
     gene            complement(20288..21322)
                     /gene="mnmH"
                     /locus_tag="PK345_02710"
     CDS             complement(20288..21322)
                     /gene="mnmH"
                     /locus_tag="PK345_02710"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF008750.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HPX33964.1"
                     /translation="MLFHPDIYQFNDLRKKFPVLDVRSPGEYTKGHIPGAVNLPLFDD
                     RERMIIGTTYQQIGMDSALLQGLDFAGKKLSSYLQQALAIVPDRVSLMHCWRGGKRSE
                     SLGGLLSLAGFEIYLLTGGYKAYRKSVRESFEKDQKIIILAGKTGSGKTEILQQLKKN
                     NQQIVDLEYIAHHKGSVFGAFGEMPQPTNEQFENDLAEAWNSLDVENLVWVEDESKAI
                     GGVFLPENLLKKMNEALVIYIDVPKKIRIERLIRDYSTYPKELITKAILKISRRLGGQ
                     NVKLAISAIDQNDFAKGIEIILNYYDKVYDYDLSLKPADKLLRLSINTLDANFGAEEL
                     LNFSKRLNII"
     gene            complement(21346..22005)
                     /locus_tag="PK345_02715"
     CDS             complement(21346..22005)
                     /locus_tag="PK345_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HPX33965.1"
                     /translation="MASKSKKFYVVWVGHNPGVYNSWDECQAQVKGFEGARYKAFSNE
                     SQAISAFRGNMWDYLKKPSKQPQLTSDQSAFEEKKSEQRHQYIAESVSVDAACGGNPG
                     IMEYQGVYTKNGRRIFYAGPFPDGTNNVGEFLAIVHALALLKSKKKTLPIYSDSETAI
                     VWVRNKHANTKLKPTNANAQLFELIQRAEKWLKTNTWNIPVLKWETEWWGEIPADFGR
                     K"
     gene            22049..22423
                     /locus_tag="PK345_02720"
     CDS             22049..22423
                     /locus_tag="PK345_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33966.1"
                     /translation="MKQILYIMAILLTIIIAMIVLFFRHDEINEFQIAIRLLAAFFLL
                     VFGIYGLYAELLFKKLRMSGKTNNLCVEASYLIQKRGILSKALLFPFLKIKSSNSLII
                     SFFGALAWVVIALIIFHRFFKS"
     gene            22426..22893
                     /locus_tag="PK345_02725"
     CDS             22426..22893
                     /locus_tag="PK345_02725"
                     /inference="COORDINATES: protein motif:HMM:NF025474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33967.1"
                     /translation="MKKFLILVSLFTFLIVGCRKTENLISFHYFDKDIWKRFDFVQFS
                     FNIKDTETEWDVWILVEHTPAFEGRSLPLSIEMITPSGDSRILDLEAYLRSTKDGNYL
                     GEAKEDYYVVKTLSFQGIRFNEKGICKFEIENYNHKYYTQGVKTLGIEFKRAE"
     gene            complement(22902..23162)
                     /locus_tag="PK345_02730"
     CDS             complement(22902..23162)
                     /locus_tag="PK345_02730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33968.1"
                     /translation="MKKKKLNNLNDIRYHKLLLKTQMRHKEEKIKKSLKKINNEIHTI
                     NFKNDILAGIVNNPSLIINTARITYDLVSRFRRWRKNRKKSR"
     gene            complement(23140..23556)
                     /locus_tag="PK345_02735"
     CDS             complement(23140..23556)
                     /locus_tag="PK345_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33969.1"
                     /translation="MDKKKIDKELNKTFANIKDYLTLKKDYYSLILTERLSKILTRFI
                     VIIVSTMLLLFFILFISFAIVSWLNRITDSEILGYVIIAFFYLALVILMLVFQKQLFL
                     NPLIKGINSMILEDEEINDILDNIENHDDDEEEKAQ"
     gene            complement(23666..23914)
                     /locus_tag="PK345_02740"
     CDS             complement(23666..23914)
                     /locus_tag="PK345_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HPX33970.1"
                     /translation="MKSSTSFLIGLLSGAAIGAALGILYAPDKGKVTRKKIAQKTEDL
                     KDDILDKMDEMKQSFGESINKVKEKVEEVKGKMQKKEA"
     gene            complement(23990..>24273)
                     /locus_tag="PK345_02745"
     CDS             complement(23990..>24273)
                     /locus_tag="PK345_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HPX33971.1"
                     /translation="SYGQYHEIVGIILLVCGVFAGANLIDNIVLQPLIYSSSVKAHPI
                     EIYVVLLIAGSLAGVPGMILAIPSYTVLRIIAKEFFSQYKIIRHLTRNI"
BASE COUNT         7911 a         4553 c         4467 g         7342 t
ORIGIN      
        1 tactccggcc tgataacgta cctttaaatc catgccttca ctcacattgg tattaaagtc
       61 aatatatgca ttcccgaaga aacgacctgt tttctcatta aaagtgttgt tatgctcaac
      121 ccagtatggg ttatcaaacg aaccaccgcg gtaataaatt tgtctggttg gttctcctgg
      181 cctatggtat ggtattccag ctaagttata gcttgaaggc gctgctaaaa ctcctgccaa
      241 cgatccgtca ttagctccgg tcaacttatc aatacctaca ttggaataat ttgatgaaaa
      301 tccaactttg aaataattgt taagtattct ttcggcattg gcttttacat tgtgtcgggt
      361 caaaccagta tttaaagcaa tacctgtttg atcagtgttg ctataaccta acgaaaaatt
      421 acctcctttt actgcctgtg aaatactaaa attattagtc attgtataac cagttctgaa
      481 ataatcattc cagttgttat aaactttagg atatacccat ggatcaaggc ctgcctgttc
      541 aagctgcata actctgtaca tgccaggatg accatgatta tttccgccat aggttgggtc
      601 gtcaggcagg tcaacaattt taggtcccca tgaatatgat gaattgttaa catatttgcc
      661 acctgatcct tgtgcgtatg ttgtttgata atcaggggtc ctggaaacaa catcaaaact
      721 gctattgtgt ccgatggaaa cgacgggttt tccaatttgg ttatatttcc cgctctttgt
      781 ggtaataatt accacaccat tggaagcacg aataccataa agcgctgcag cagcctgtcc
      841 tttaagcact tcaatgcttt cgatatcagc agggttaata tcaatagcac ggttcgaaat
      901 atcggaacca gtaacgctgt ttccggtaga gtatgccggg gttgaagaaa ttggcattcc
      961 gtcaactaca tacaaaggtg tattatttcc tgtaaaagaa cgtgcccctc tgatttgtag
     1021 ctgagacgat gctcccggca tcccgcttga aggtttgata tccactcccg atactttccc
     1081 ttgcaaagcg cctagcaaat ttacgttacc agtacgaata agatcctcat cggcaacaga
     1141 ttgaactgca tatccgagag acttcttctc gcgggagata cctaaagctg taacaacaac
     1201 accctcgata gcggttactt caggttgcat agttacgttg ataacatttt cggcgccaat
     1261 cagaatctct attgttttca taccaacaaa agagaattgt agaatcttgg catcagcagg
     1321 aaccaacaag ctgtatcttc catctaaatc tgtagttgtt cctacagttg ttcctttaac
     1381 cactactgat acaccgggga ttgtacttcc gtcatcggca tttgtcacac tacccgatag
     1441 ggtcctggtt tgcgcctgtg cccattgcac tccgataaat atcataagtg caaacaaaag
     1501 tgtaaatctt ctcataataa ttgctttggt taattaattt attaataatt agatagttaa
     1561 aaactaaaaa tctcatttac tcacattgtc attatccttt atttcaaaaa acgcggccaa
     1621 attaatacat tttcatttat tgaacaagaa aattaaaaaa ttttaatctt ttttcattcc
     1681 acttctttga ttatcatatt tttatcttaa agcaaaaata agcatttaat tgcttataat
     1741 aaatagtgaa aagtaaaact tagcacctca ttatattgtc ctctattaag tctgtatgtc
     1801 tggcctcctg agtgtggcct tactggaata accgaattgg aatatcttaa gttaaatctc
     1861 catcgttgat taatattata gaataaacca atattcattg ataaatcgaa tagatgaaat
     1921 ggattgtcca gcaactgata atcagcttcc tcatgttgac ttactaagac gcctaacgaa
     1981 ggaccggctt ctaatgtaaa tagatgatga aaatcccatt tatagtgtaa aaacaactcc
     2041 acataattga tccttagcaa gtatgaagaa aaattatttg cagaatctgg attcatacga
     2101 cttccttttt gtataaaatt caactccatt tgcagcgacg atttcggagt tatatatcta
     2161 ttgacaaatc ctccaatata tatacctgct ttgtttggcc tctcaatacg atcggctgaa
     2221 atttcactgg cagaaagccc cgcaatcaac cctccgttaa attcctgagc acataataat
     2281 gttggtacaa ataaaaaaat aggtattaat aaaaggtaaa agtcatgctt cataggcaaa
     2341 ttgattaagt taattagtca gtaaaaatag tattattctt agaaataagt tttatttatt
     2401 cattttatgt attaaattac cataactctt taatttaatc atagtcattt ttagattttg
     2461 tgattgaatt ttacattttc aaacaattag ctttaaatgg accaaaatag ggatttcgat
     2521 atattgtaaa cacgaaaatg gaatattttt gctgttaaat actttcacaa gtatcatttc
     2581 atcctactta tgatttaata taaaagtaaa cgatatcaat atattatgca aaagatcagt
     2641 actcatccca tccttgaagt tccgttaagg gaaaaaacaa cctttacctt caacggtcaa
     2701 gagattgaag gggaaaaagg gtttaccatt gcggcagcgt tacatcaggc cggatttccg
     2761 gtacacagtc atagtttaca ggatcgaaat cgttcgttgg agtgtggtat tggtaaatgc
     2821 ggagcttgcg aaatgttagt agatggcaaa gttaaaagaa tttgcataac aaaagttgat
     2881 ggaataaaag aggtaacaga aatacccaag gaatacacac ctaccataga atatattaat
     2941 gaaaccgaac ccgttaaagt ttacaaaact actgtggcaa ttgttggggc agggccggca
     3001 ggtttagctt gtcgcgaaat actcaaccaa cacaacatct tcaacatagt aatagataat
     3061 aatgataaaa ttggtggaca atttttaatg caaacccatc agtttttctt ttttgaaaag
     3121 gaaaaaaagt ttggaggaat gcgaggtttt gacattgcaa atacactggc aggtaacaat
     3181 cataccggaa tatttctcaa ctcaacggtt tgggacattt tagaaggtaa aagattagct
     3241 gtaaagaata ttttgactga tgatgtgtat tatgttgata gtgaatatct tgttgtcgct
     3301 actggagctg tacccttttt gcccgcattt aaaaatgatg atttacccgg ggttttcact
     3361 gcagcagtaa tgcaaaaaat gatgaacaat gagttcactt tacttgggaa aaacatccta
     3421 acagttggcg ccggaaacat tggatacctg acttcctatc aattgatgca ggcaggcggg
     3481 tgtgtaaaag caattatcga agcacaaccc aatgagggag gctttccggt tcaagcaaac
     3541 cggataagaa gacttgggat accaattatg acttctaaaa tacttcttaa agccatacca
     3601 aataaagaaa acacagggat aaccggcgct gttattgccg attgtaaaaa ctttgaaccc
     3661 atacctggaa ctgaaaaatt aattgatggt attgatgcaa ttaacatctg tactggtctt
     3721 gaatctgatg atcaattgtt aattaaagga agggagattt ttggccgtaa tgtttatggc
     3781 tccggagatg caatacgaat tggtgaagga acaagtgctg tattgaaagg aaaacaagtg
     3841 gcttttgaaa tcttagatga tttagcggaa catttcgact ataatgaata tttggcaatc
     3901 tcaaaggaat atattgattc tcagcaacat cctgttagag tgatcgaaag agcctttgat
     3961 ccgacagaag aaagaatttg gcaaaagcct tttgtgatta ttgactgcct atatggattt
     4021 gcctgtaatc catgcgagtt ttcttgccct caaggagcta tccgaaaaac ctctaccagt
     4081 acggtaccta caattgactt tgaaaaatgt ataggctgta tggagtgtgt ctatcaatgt
     4141 cccggactgg caatttttgg ttacaacatt aataaagatt ggatattcct tccaatagaa
     4201 tatgatgttg atgaaggctc tgacgttttt ttagttgaca ataacggatc agtattaggc
     4261 gaaggtgttc ttgaaaaaat attgaagaaa cccaataaaa caaatgttgc aagagtaaaa
     4321 tctaaagata ttcatggccg ggatttactt aacgtaagag gttttattgt aaaatcaaac
     4381 tatcccgaac cattaaattt gcagcctttt acaaatgaaa ttcaaaccga acaatacatc
     4441 tgtcattgcg atgatgtacg gctggatgaa atactggaag taattggcaa tcgaaaattt
     4501 atttcggtag atgaggtaaa acatactact cgccttggaa tgggagcgtg cagagggaaa
     4561 cggtgtatta aaagattaaa attggtttta aaaagtagtg ggatttcaat aattggcgat
     4621 ccaaccccac gcgcccctct ttccaatcag gtttctttag gtgatattta tcccaaaaaa
     4681 gtcaaagaac aaattataat accccttaat agccgaaaaa caagcaaaat aaaggttgaa
     4741 gcgcttgttg ccggtggtgg gattggcggc agtgctcttt tccgttacct tgccgaagca
     4801 ggtctgaaac cagttttggc aaatcatgga cgcggctctt catggcgaaa tatagcagga
     4861 ggtcgcccaa atttcagtct gcccgaatta tccgacatcg cctcacaaaa cttcgaaata
     4921 tttaaagaac tgcaaaccat aagtaacatt gatttccgta caattgatta tgtaaccttt
     4981 gcacacgaca accagttact cagcgcatta gaagcttcta tggcatggtc tgatgcatac
     5041 ataatcgaac ctaaagattt tgtaaaacat atctctcccc atttcaaccc taaccttaaa
     5101 acttataagg cagcaatggt aaccaaaaat tgctggcaag ctactcccgg aaaagttgta
     5161 gatcttatcc ggcaaatagg gctaaaacat ggcggtgtaa tttgcgaaga ttgtgaagtt
     5221 attgatattg gtaaaaccgg taatacttat actgttttgg taagaaatca tgaaaaagag
     5281 tatgtcgaat accaaacaac tcgctttatc aatgcaatgg ggtttcaagg tgatcatttt
     5341 gcgaaaaaaa taggaataga aacgggtgtc tatcctgtaa aacatcaggc attcatcaca
     5401 cgacgcctac caatgatggg tatcaacgga atacctttgc caatgataat tgaccgaaga
     5461 caatacaaag gttttatagc agtttatgga caacagttag gagaaaccgg ccaaataata
     5521 ggttgtgcat cacctcacat cgagccttat gaaactgaca aaaaccttaa aatcaattca
     5581 aaagattttc tggaaattgt ttcagagatg tttgttgatt ggatcccgga attatcatca
     5641 gtgggttttc aggcagtatg ggctggttat tatgtcgaac cacgaatgat acttgatcct
     5701 gaaaacgggt tattcttagg attaagagga cagggtttta tgctcgggca atatttagcc
     5761 aaaatgtatg tggataaaat tatcggaaat cctgtgccta cttattttga cagacttaca
     5821 ttaaaaggtg atggattgct cgaaaaggct tttaaataaa ctgaaaatca atagtttttg
     5881 atttttggtt gttttattat atttgcaaaa acaattaatc ttattaatca tgaatccttt
     5941 ccgtacctct atcttgaaca caattcttct gtttgttttt ttatcaacag ttattattgc
     6001 tctcacaatc aaatttacaa agactaacac aaccgttgac agggaggctt acgaggtttt
     6061 ccttcgtgaa aaaatggata aaataccttt agctgacgct accgagtcaa aaaaaatccc
     6121 taaacctgct caacctgacc gggcagctat tcaaaactat tttatgactt tggacccaaa
     6181 aacaggagtt gtacccaaag agagactaat gcaagtttat aatgatttaa aatctaatcc
     6241 taaaaaaaac agaatcaacc cactcgaatg gtcaggtaca tcagccaata tgggaggtcg
     6301 tacacgggca ataatgtggg atccaaatga tccgcaaggg aaaaaagtat gggctggggg
     6361 tgttactgga ggattatggt atcgcaatga tattaccaat aataataatg aatggcaacc
     6421 ggttgacgac ttctggagct ctttaaatat tagttgtatc acttatgatc caaacgatcc
     6481 gatgattttt tacgttggta ccggtgaagc tgagacagct ttaattattt atagagaatc
     6541 ctcaggtgtc ggagatggga tctggcgttc aactgatggc ggacaaacgt ggtcgttgat
     6601 ggaatcaact atcgcatttg aatacgttac tgatcttttg gtaagagatg aaggggggca
     6661 gagcgtcatt tatgcaggcg tagcatcagg taattataaa ggaagcattc attacagtga
     6721 accatcggat ggtcttttca ggtcagaaga taatgggcaa acctggcaac aggtattgcc
     6781 cgatattcct gagtacgggg agccttatgc tgtttcggat attgatatgg gtgcagatgg
     6841 aagaatatac gttggcacaa tgccaaatgt agatattgaa ggcggcgctg taatactata
     6901 ctcagatgaa ggcctcagcg gaagttggtg ggtttacgat aattatgttc aaataatcga
     6961 aaacaaccct caatataaaa tccccggtag agtaatggta gcccctgccc catccgatcc
     7021 taacattgtg tacgcacaga tcgctgccgg atacaatagt tattttaatt attatcgagg
     7081 ccgttatata cttaaatcaa caaacaaggg acaaacttgg tcatcagtaa tgattccgga
     7141 atcaacttgg tcaacattgg cgtggcacgc ctttacgcta agagtggatc ctaataatgc
     7201 caataatatc tttacaggtg gtttggatat gtggaaatca aacaacggtg ggtcttcatg
     7261 gacaagaata tccgattggt cactaatgta ttatggcggc ggagatcagt atttacatgc
     7321 cgaccaccac gatattcaat ttaaacctgg cagctccact acttttataa catgcaacga
     7381 cggaggagta ttttatacag aaacagccac atcctctttc cctgtttttc aagaaaaaaa
     7441 tcagggttac aacacgttac agttttatac aggagcaata catccatcag caggatcaac
     7501 gatatatgca ggaggattgc aggacaacgg ctctctgctg tacaacggac aaccattaac
     7561 tataaatgat atggtaagtg gcggcgatgg cgctgcctgt ttttttgatg ataacgatcc
     7621 actgcttatc acctctgtgt attataatag gtattatgtt tttatgaatt acaacatgta
     7681 tacctactac gactatgaaa gtggaatatt catcaaccct gcagattttg atgtaaaaac
     7741 caacacgctt tttgctaatg cagttagttt ttccggcgga aaccctaaca ctatacttcg
     7801 gataattaat attccttaca atgcaacagc aagtttcacc ggtttaaata ccaacacaaa
     7861 tgtctattat tcacatatta aagcatcgcc ttatgccgaa gcagggaaat ctacaatctt
     7921 tgtcggcaca caatcgggca gagtttacaa agttataaat gctgaagctg taccacaaac
     7981 tgttgaaatt acaggcacga atttcccgac tgcttacgtt tcgtgcatag caataggttc
     8041 aagtgaagat atactactgg ttactttttc aaattatggc gttgagtcat tatggcaaac
     8101 gattgatgga ggcgaaacct ggcaaaatgt acagggcgac ctccccgata ttccggttcg
     8161 ttgggcatta tatcatcctg aaaatgacaa tcaggttatg ttagctacag aacttggaat
     8221 atggacaacc aacacaataa attcaccgga agtaatctgg aaacaagatg cacaaggtat
     8281 ggctaacgta agagttgata tgcttacatt gcgcaactcc gataataccg tgctggcagc
     8341 aacacatggt agaggctttt acaccactga atttaaacta atcacaggcg ttgcaataaa
     8401 taaaaacaac aacgaaacga ttaatatttt cccaaatcct acaaacggct taatcaatat
     8461 agactttgga agtatggatc agaaatcaat aaatcttaaa ttgattaatg tttcaggaaa
     8521 ggtggtattg gcgaagaaac ttccggaggg caagtttcac acacttgatt tgaccggatt
     8581 tccaaaaggt aattatctga tcagcatttt ctcgacagag aaaaaaataa gccggaagat
     8641 tgttttacaa tgattatagt ctctaattga atttgtaaac ccgttttatc caaattggga
     8701 gtaccagtgc gccaataatg atatatggat aataactcat caaccgccaa agtagcgcaa
     8761 cggccatatt aagaccggcc ggaataaaat cgccgaggaa atctgaaaac agaaactcag
     8821 ccacaccgct gcttcccggc gtagggctta ttagcataat gacccacata acaagttgac
     8881 gggcaaagat caaaaaatga tctccccctg cagtgatggc gtagattaaa aaattgacca
     8941 tcaaaaacct tgatgtccac gaaatccaag tagaaagaga agcccttgcc caaaaactaa
     9001 aagatttatg ccgcatttcg gctgaagtta taataatgtc atcaccagcc tgtgcagcct
     9061 gaaaacgcca tttccgaaga aaaggaatct taaaaattct gaggagaatc catttaaaac
     9121 cccgtggttt aaaaaaaaca ccataagaaa ttatcaatgt caagaatact ataaaaaaat
     9181 aaccaataac aaaaacccct tgtatcccaa gtttcgaatt gatgaataga aaactcgatt
     9241 cacttttaaa aagtaaagat gtgccagcaa aaagtaaaat cagtggaacc atgatgatat
     9301 aaaaaagctc atccaataaa gctgttatca taacaatggc tgtggcacga ccaggtttta
     9361 ttccctcctt agtaacaata aacagcgcta ctgctgaacc tccaacaaca cttggggtta
     9421 cagacgaagc aaattcccaa agcataataa catgaaacga gtttcgccag ctaagctgtt
     9481 tatcggtgag caaacgaata cgatacatat aggctacatc acgcatgaca accatcgtaa
     9541 aagccatgaa aagccaaaac cataattgaa aactaggctg aatctcaaaa aagggctttg
     9601 gatcaaaatc cttaaatgaa aaataaccaa ctactcctaa accaatgatt actggaataa
     9661 tcacccgctc aaacctgaaa atttttaaaa ctttattttg ttccgactgc attcgatatt
     9721 attcagatat attctttcgc aaaaatgata aagatttcca acaagctgta tcacaagaat
     9781 gaaaattttt aatttcatca aagtttgtta gttcgaagca ataaaaattt taatgagcaa
     9841 acaattatat aggattggtc ttaaaatttc cctttcagcg caaaaatact ttgtattagt
     9901 tgataggtgc ttatttcaaa aaattgtggt ttctaaaaaa atcaacatga ttaggtcata
     9961 ttgtctgatt ttgtaaatta atctgacata ttgtcgcggc gatatttact taaaagcaat
    10021 gttttgggtt ggtataaaaa ttgacaaacc gctttcacaa ttaaaaataa caaaagttta
    10081 actataatag aaaggaataa aattatgaca atgatgagat ggaatccaga cacgcctttg
    10141 agcgatttgt tcgagaattt tttcagaagc cattccaacg aacgcatgga aagaaagcat
    10201 tacgattgtt caccatcaac caatatcctc gaaaaagacg atagtttcga gctacacatg
    10261 gcggtacccg gcgttaaaaa agaagatgtg aaaattgacc ttgagaagaa tgtcctaagc
    10321 atttcttcag agaagaaagc cgaaaaaacc gaagaagtag agggtaaata tagcagacgc
    10381 gaatttctct acggcacctt ctgccgttca tttactttac ccgaaacaat taacgttgat
    10441 aaaattaagg ctgatttgaa agatggtatt ttaaaagtaa ttctgcctaa aaaagaagaa
    10501 accagaataa ctaaagagat aaaaatcgca taataaattt ggcaaaatga taagaccggt
    10561 tcaaacactg ttttgaaccg gtttttttat tcccagccaa ctaaaactga tcttaaataa
    10621 aaaatgaaaa atcctctata gcatactttt tacattaatt ttgtgtttca acttccaaaa
    10681 atttttgctt gaaaggaaaa ttctttttgc tgccagtttt actcctgttg ataagtagtt
    10741 gcaggataac acatgaactg aaggatgatg aaaaaatcct tgtaagaaat gttataaata
    10801 ttgacgaaac taaaatagaa cctaaaaaac tgaattttga cccgaacgac ctttattcga
    10861 ttctaaatca aaaacctaac aaacgatttc tcggagtttt cagatttaga gaatatattc
    10921 acatcaaaaa ctatcaaaaa gcaaaaaaac cttcattcaa atattggcta tcaaaaaatt
    10981 ttggtaaaga accggtattt tatgacgagc ttgcggccga gcgttcggtg agccaattgc
    11041 aattgtttct aaacaaccaa ggttatttta attctcaaat cgaaaatgag gtctctttta
    11101 aaaagcataa ggcacaggct atttacgcta taaaaatagc gcaaccctac cagataaatc
    11161 aaattgaata tcaaatccct gatacggcaa tttcttcgat cattataaat aataagagta
    11221 aatcattagt taaaccaggc gctatttata atgtaaacct attgaatgac gagcgttatc
    11281 atataactac cctacttcgt aatcatggct atttttattt tacccccgat tatgtttttt
    11341 ttgaagttga tagtgcattc aataatcatt cattaaatat ttatacaaac atcagcaatc
    11401 ccaaaatccc gataaaagat tatcctgata gcatttctga aagccttcac aagaaatatt
    11461 tcatcaatcg gattgctatt aatacagaat tcagtccgct aagaagagat acaacgcaac
    11521 tgatggctta ccgcgacacc acttcgaaag acaatagaaa caggtatatc tattattaca
    11581 gagataaatt aaaggtattc ccaaaagcct tgcgcaatca aataagtttt aaaccagatg
    11641 agctttatac cgaaaaagat caagaaaaca cttatcgcca attatcaggc ctgccacttt
    11701 acggatacac aaattttagt tttacaccta cggcagtcaa tgatttttca aataacttag
    11761 ctgggtacaa gcttgatgca cttatcaact tatcacgaaa accagtgcat tcgttttctg
    11821 ttgaggcaga aggaaccacc tccggcagca cattaggaat tgcaggaaac gttgtgtatc
    11881 aaaatcttaa tatatttaaa ggtgcggaac tgttaacttt gaaagctaca ggaggccttg
    11941 agtggcaaca aggcgggccc gaaactttag atgttttttc atttataaat accattcaag
    12001 caggagccga agcccgtatt gattttccga aatttttgat tccattttat agtggacaag
    12061 tttcgaaatc ttccaggccg agaacttcat taatgatggg tatcaattat caaaaaaggc
    12121 ctaactacac acggtacatt accaacgcct catttggcta caactggaaa atgggaaaat
    12181 atgtttatca cagctttgta ccggttgaat taaattcggt atctattttt cctgattcga
    12241 gctttgtcag actattgaaa gatataaacg atccgcgcct caccaaccaa tacactgatc
    12301 atttaatctt attgtccaaa tacagtttca tttttaataa tcaagagcga aacttagtta
    12361 aaaactttac ctatttccga tggggaatcg agtcggcagg taactttatt tacattctaa
    12421 atttgattac cgagcctaag ataaataaca aaggggaaac aatactatgg aatattccta
    12481 tttcacaata catcagaaca gatattgata tccgacgata tttaacactt gatgaagata
    12541 ataccatagt aatcagaggg ctggccggta tagggatacc ttacaaaaac tcattagtgt
    12601 tgccttttga aaaaggcttt tacgccggcg gcgcaaatga tatgcgcggt tggaaatatc
    12661 gctcactagg gccgggagcg taccgagaca ccaccaaaag atattttgaa aaaatgggag
    12721 accttatttt agagctaaac ctggaatatc gttttccaat ttatggattc ctcaaaggcg
    12781 ctattttcgg ggatttagga aacatatggc tacttacctt ttcagaaaat tatccgggag
    12841 gtctttttaa atggaaagat ttacccaacc agattgcatt tagttcgggt gccgggttga
    12901 ggtttgattt ttcgtatttc atcttccgtg ttgatttagc tgctccgatg agggcccctg
    12961 gctatcctat cggagaacaa tggcgaataa aaacccttaa accaaaagac attatctgga
    13021 atttcggtat cggttatcca ttctgatgaa actttatcct aatgcaatat cactggggac
    13081 atcaacgcag atttaactca tatgcagagt attttaaaag ccactttgga acgagagttc
    13141 aaaaagtttc ggttgatgcc ggatttacgt gtccgaatcg ggatagctta aaaggaacag
    13201 gagggtgtac ttattgtaac aacgccgcat ttaacccctc ttattgtaac ccgacaaaaa
    13261 ccatcaaaca acaaatagaa gagggcattg agtttcacag aaaaagatac cgaagagctg
    13321 ataaattcct ggtctatttt caggcttttt ccaacactta tgcacctcta aatcatctta
    13381 aaacaattta tgaacaagcg ctgcaacaag ataaagttgt cggattggtt atcggtacgc
    13441 gccccgattg tatagatgat gagaaacttc aatattttgc cgatctggct aaaaaaaatt
    13501 atatcatcat tgaatatggt ttagaatcgt gctacgatcg ttcgctacaa aatataaatc
    13561 gcggccatac ctttgccgat tccattgaag ctttaactaa aacatccaag aaagggatta
    13621 aaaccggtgc acatattatt ataggactcc caaacgaaac agatgacgaa atcataaatc
    13681 aggctgatat actatccaaa ctaccaataa caaccctcaa actacatcaa ttacaaattg
    13741 caaagaacac aataatggaa attcaataca ggaaaaatcc taataatttc agatttttcg
    13801 aaataaatga atacatcgaa cttgttattg cttttttaga aagacttaat ccaactattg
    13861 ttttagaaag atttgcaggc gagatgcctc ctcggtttat tgctggccct cactgggggc
    13921 caatacgtta cgatgtaatt ttacaaaaaa tcgaaaagcg tttacttgaa aaaaacactt
    13981 ggcaaggaag gctttaccag gagatacaat ctttctaaaa aaaaaattga aattattttg
    14041 tttgttatgt ttaagataaa aaatataaat ttgcactgaa aataattttt ttaactaaaa
    14101 agtaaaaatc atgaaaaagt acttactatc aatcattgca atgtttttaa tgggattcat
    14161 tgcagtaggt caggttacca tttatgagga caatttcgat tcctatacag ctggtgaaaa
    14221 gctgaccatc gaaaatcctg aaaactggac aacatggagc aatgcaccag gtggtccgga
    14281 agaccctgta atctctacag attattcgtt cactaacccc aactcagtaa agattacaac
    14341 tgacaatgac gttgttctgc ttcttggcgg gaagactgtc ggaaaatata acttaggatt
    14401 caaaatgtat atcccagccg ggaatcttgg atattttaat gttttacaag attttgccgg
    14461 atcaggtagc acatgggggt tacaagtata ttttgacctt ggtggcgcag gtttaattga
    14521 tggtgatggc gcaggtgcag ccagtttcgt ttttggatat gatcaatggc ttaatcttaa
    14581 ggtatttgtt gatcttgata acgactggtg caaagttttc ttaaataatt cgcaaataca
    14641 tgaatatcaa tggagtaagg gaacgtttgg cacaggtatg cttaagcttg atggaatgga
    14701 tttttatgga tgggatgatg gcggaactcc tcttttctac gtagatgatg tatattacga
    14761 aatggtaact gaaagcctga ttgttgaaga tttcgaatct tataatgcag gtcagcatct
    14821 cgttcaacaa gccattgctc aaggaaaaga ttattggaca acatggaaca actccccggg
    14881 aagcgctgaa gacccaatgg tctcttccga tcactcagta agtggatcaa aatcagtttt
    14941 aattgaaggt actaacgatg ttgttcttct ttttaatgat cttgttacag gcgcctatgc
    15001 aatcgagttt aaagtttata taccacaagg attttatggc tattttaacc tccttcaaga
    15061 ctttaacggt ccaaatagtg agtggggaat gcaaactttt tttgatcaag gtggattagg
    15121 aaccgttgat gcaggcggtc aaggagccgg ggtattcacc tacgcctatg acacatggat
    15181 ccctgttaaa attgatgtgg atttaaataa cgactttgct gaactgtttg tcaatggtaa
    15241 ttctgttgtt acctgggtat ggagcggagg aactttcggt caaggcgctt taaataaact
    15301 tgatgcattt tctatgtacg cgtggaatga aaatggaacg ccaaaagcat attttgatga
    15361 tatcagcctt acacaaacag ctcctccagg cggtgatcct gaaattaatg taagcccaac
    15421 ttcatttacc gaaactctcc aaaacggcca aaccagccaa aaaacactca caatttccaa
    15481 taccggcgtt gtaaaactta actataacat cattgtaaac tttgatgttg acgatggaga
    15541 tgccgaaact gaatacacgc aagaagttgt tatagagcct gaaacaacaa ttaccggaaa
    15601 tgacattgcc gttataaaag ctgaaaagag tcaaccagga ccttcagtta ctgatgatga
    15661 ggtattatta cattatgatg gcgactacag ttcagctgtc ggactaacaa acggaggaac
    15721 ttttgaaatt gcagcaagat ttcctgcagc cttaacaggt cagtatatag gaatggaact
    15781 aacacaggtt cgggtgtata tcaatgatcc agcaaatggc tataaaataa aaatttatgg
    15841 tcagagtcat cctgacaaac caggtgaatt actttatgag caacttttct cagcaaatgc
    15901 aggcgcctgg aatgaagtta atctttacac cccactcctc aacaccggag gcgatatgtg
    15961 ggtagcatac gaaatcgttc aaaacgttgc tggagtatat cctgctggaa ctgatgcagg
    16021 cccggcacat gccgatggcg actggattaa aacaggctct acatgggcaa aattatcagt
    16081 aataaatcct aatataaacc aaaattggaa tatccgtgca aaacttgttg gtgatcctat
    16141 gcaagcatgg ctttccgttg atccaagttt tggttcaatc gagcctaata tgtccgaatc
    16201 aattaccgtt aactttaatg caggtagctt acccgaaggt acttatcagg ctaatattaa
    16261 cattgcaagt aatgatccgg taacacctct agtgtcagtt cctgtaactt taaatgttca
    16321 aggcggagga tgcccgcttc caccccccac caaccttaca ttacaacaaa caggtactaa
    16381 cccctataat gttgtactca actgggttgc tccagaagag ccaggcggca caatacgttg
    16441 ggatgatgga ataaactatg acggtatcgg attgacaaat ggcggtactt tctccgttgc
    16501 agctaaatgg gaaccaggcc aattaacaga atatattgga ctatacctta aacaggttga
    16561 tcttttccca agatctgcaa ctgctactta cgtggtgaaa gtatggaccg gtactgccgg
    16621 aaatcaacaa ttagttagcc aaacagccac actaatttct gaacaatgga atacggttac
    16681 aattaccaat ccagttaaaa ttaatgcagg aatgtaccta tacattggtt atgaaacaac
    16741 tcatggggca ggtgaattcc cggccggtgc agatgcaggc cctgcagtag ctaactttgg
    16801 cgatttaatc agtactggtg gcgcatgggc atctatgtca atacaatttg gcctaaacta
    16861 taattggaac atcgccggta ttatcggtgt cggttcagat aatgctacac ctgccgagcc
    16921 aatagttatc acaccaaaac ctggcgcttc ttatggctta cctatagcag gaaacttagg
    16981 aaaaaccaat aatgcacagt gggttaataa ctcaagagct ttagaaggtt acgatattat
    17041 gagagatggc cagataatcg gttccgttgg gtcaacagtt actacctata ccgataataa
    17101 tgtaccatta ggtgcgcaca cttataaagt tggcgcccac tataccgaat gtactgcatg
    17161 gtcagatccg gcttcaatag gagtatcaat ttctgagcct accgaaaaca taaacacttt
    17221 aagtatttat cctaatcctg caaatggaga agtaaatata agcagcccga cagatatgaa
    17281 acaactgaaa attataaact ataccggtca agttgtatat cagtcacagc ctaacaacaa
    17341 tcatatcata atcaaaattg ccgatctatc agcaggaatg taccttattc agattgaaac
    17401 aaccgaagga tggacaacaa gcaaattggt tattaaataa attaattaaa ttttcttttt
    17461 aaaaaaaagt ctcgaaatca ttttcgggac tttttttatt ttgatttatg aatgacgatc
    17521 ttacattttt tgataaagtt tatcttgttg taaaacaaat tccttttggt agggtaacct
    17581 cctacggcgc aatagcagct tttttaggaa ccaaaggttc agcaaggatg gttggctggg
    17641 caatgaatgc ctcacataac tatatagaaa atataccggc acatagggtg gtaaaccgaa
    17701 atgggatgtt aaccggaaaa aatcattttg gttctccaca cctgatgcaa caactacttg
    17761 aatcagaagg aataaagatt gaaaacgata aagtagaaaa ctttaaagaa ctcttttggg
    17821 atccggcagt tcatctcaaa atataaatag gtcttgtatt agagtattgc agttaaacag
    17881 ggaagtttgt ttattgtttc gcattagaga tttttaggca caaataactt taattcaata
    17941 tgatggaagt ttattatttt tctaataccg aatttgacgt taatcaataa aaaaacaatt
    18001 tgggctaaac tattgatttt atgaagctaa tccagggtta ctgtcgttaa atgcttactt
    18061 taaatttatt acttgaatta ttcctgttga tgtttcgtca gtttcaatta tttttccaat
    18121 aatttcggcc tgtttgttat gattttcatt tagttttcgg ataaacccag ctccaaatgc
    18181 ctccggtata gcaatcaata gacctccgga ggtttgtgca tcgcataaaa ttagttttgt
    18241 aatctccgga atattttcgt cccatttaac atttgccgac acataatcga ggttgtttct
    18301 tgtaccccct ggtatggctc ctgctgtagc aagttcataa actccttcta ttattggcac
    18361 ttttgaggaa taaattatgg aacctacacc agaagcttcc atcatctctt taagatgccc
    18421 aagaagtcca aaaccggtaa tatccgtgca ggcatttacc ggaaaaagac tcattaagtt
    18481 tgcagcatca gcgtttaact ctaacatcac attacgggct atgagtgaaa tatttttctt
    18541 gacaatacct cgttttacgg ctgttgagat gattccggtt ccgattggtt ttgtcaatat
    18601 caaaacatct cccggctttg ctgtagcatt ggtaagcact ttgtcaggat gaaccatccc
    18661 ggttatagcc attccaaact taggctcaat atcttcaaca gtgtgtcctc ccaaaatgga
    18721 tattccagct tcggcagctt tatctgaagc gccttttaaa atttctttta aaacatatat
    18781 cggtaagcga ttatccggaa atccaacaat attgagtgca aaaagcggct tagcgcccat
    18841 tgcataaatg tcgctcagcg agtttgcagc agcaatagcg ccaaaataat agggatcgtc
    18901 aacgataggt gtaaaaaaat ctacagtctg tacaatagca gttgatttgt tgatacggta
    18961 aacggcagca tcatccgaag ttccggtacc tacaattaca ttctcatcta ttgacaaagg
    19021 aaggtctttt agaatttgtt cgagatattg tggtcttatt ttgcatgcac agcccaaacc
    19081 atgagtgaaa tcagtcagtt ttatttcatt agtttccatt ttggttttat cctgaattac
    19141 ttcatcgggc gacagccgct ttactgcggc aattataagc tcagctgcct tttccatatc
    19201 ctcaatggcg ctatttttac ctgtcgaaaa acgtattgtt cccatagcaa aatcaactgg
    19261 aacgcgcatt gcagtaagaa cctttgatac atcaatctga tcggcatggc aggctgctcc
    19321 ggcagaggca gctatttctt tcaattcgct aagtaaagtg ttggcttcta tattcggaaa
    19381 actgatactt aaggtattag ggagtcttaa atcgggatgt ccattaagtc tgatgttggg
    19441 taaggctgta aaaagcagag acgcaagttt atcacgggta agcatcattt gctttatatt
    19501 agtctgaaaa tcacgtttag caacttccgc tgctttacca aggcctacaa tttcaagtac
    19561 attttcggtt ccagcacgta agttttgttc atgattagct ccatgaatca gcttttgcag
    19621 cttcacacca cgactgataa acagcgcacc aacaccttta ggcgcataga acttatgacc
    19681 ggcaaccgac aaaagatcaa caccaagttt ttgtatatcc gtttcaattt taccaattga
    19741 ttgtgctgca tccgaatgaa agagaactcc atgcttcttg gtaatctctg caattttctc
    19801 tataggctga ataactccag tttcattatt agcatgcatt actgtaacca aaatagtaga
    19861 tggcaatatc gctttttcaa tattttgagg atctactatg ccaaagtcat caacaggtat
    19921 ataactaact ttgaatccat tagcctctaa aaaacgacac acttcgctca cggctggatg
    19981 ttcaatagtg gtagtaatta tgtggtttcc tttattacga tattgaaatg caacgccttt
    20041 gatagcataa ttgtttgctt cggtgccgcc acttgtaaag ataatttcat ccgtatggca
    20101 gtttaacaat tctgagagct gtcggcgagc gttttcaaca gcatttctgg ctgcagcgcc
    20161 aaaagaatga ctacttgagg gattaccaaa ataatctttc aaatatgggc gcatagcttc
    20221 agcaacttca tccccaatag gagtggtggc attataatca aggtaaatat tatactctag
    20281 cttcatttta aataatgtta agtcgtttgg aaaaatttaa taattcttca gcgccaaaat
    20341 tagcatccaa agtattgatg ctaagcctta ggagtttatc tgccggtttt aatgaaaggt
    20401 cataatcgta cactttatca taatagttaa gaatgatttc aatcccttta gcaaagtcgt
    20461 tttgatcaat tgcacttatg gcaagcttca cattttgtcc gccaagtctt cggctaattt
    20521 ttaaaattgc ctttgtaatc aactcttttg ggtatgtaga ataatcgcgg attaaacgct
    20581 caattctgat ttttttcgga acatctatat aaatcactaa tgcctcgttc atcttcttca
    20641 acagattttc cggaagaaaa actcctccga tggccttgct ttcatcttca acccaaacaa
    20701 gattttcaac atctaaacta ttccatgctt cagccaggtc attttcaaat tgttcgttag
    20761 taggttgtgg catttcgcca aacgccccaa aaaccgatcc tttatgatgg gctatatact
    20821 caagatcaac gatttgctga ttattttttt tcaactgttg aagtatctcg gttttgccgg
    20881 aacctgtttt tcctgccagg ataattattt tttgatcttt ttcaaatgat tccctaactg
    20941 attttctgta tgctttgtac cctcctgtca acagatatat ttcaaaaccg gccaatgata
    21001 gtagccctcc taagctttcg ctacgtttac ctccacgcca gcaatgcata agcgacaccc
    21061 tgtcgggtac gattgccaaa gcctgttgaa gataagatga aagctttttg cctgcaaaat
    21121 ctaaaccttg tagcagagct gaatccatac ctatctgctg ataagtggtg ccaattatca
    21181 tcctttctct atcatcaaac aatggaagat ttacagctcc ggggatatga ccctttgtat
    21241 attcacccgg cgacctgaca tccagaaccg gaaatttttt gcgcagatcg ttgaattgat
    21301 aaatatcagg atgaaatagc attttacctt ttttttaaaa aaaatttatt tacgcccaaa
    21361 atctgccggt atctctcccc accactcagt ctcccatttt aaaacaggaa tattccaagt
    21421 attggttttt agccattttt ctgctctttg aataagttca aataattgag cgttagcatt
    21481 agtaggtttc aattttgtat ttgcatgttt atttcttacc catacaatgg ctgtttcaga
    21541 atcactataa ataggtaacg ttttcttttt gcttttcagc aatgccagtg catgaacaat
    21601 cgccaaaaac tcacctacgt tgtttgttcc atcaggaaaa ggaccagcat aaaaaatcct
    21661 tctcccattt tttgtataaa caccctgata ttccattatg cctgggtttc caccgcaagc
    21721 agcatctaca gaaacacttt ctgctatata ttgatggcgt tgttctgatt ttttttcctc
    21781 aaaagctgat tggtcagagg ttaattgggg ttgtttcgat ggctttttta aataatccca
    21841 catattgcct ctgaaagcag aaattgcttg cgattcgtta gaaaaagcct tataccgtgc
    21901 accttcaaag cccttaacct gtgcctgaca ttcatcccaa ctattgtaaa cacccggatt
    21961 atgaccaacc caaacaacat aaaacttctt tgactttgat gccatttttt atttacgtta
    22021 attttgcaaa aatctaattt cttcaaaaat gaaacaaatt ctttacatta tggcaatttt
    22081 gcttacaatt attatcgcca tgatagtttt attttttcgc catgatgaaa taaatgagtt
    22141 tcaaattgca attaggttat tagcagcttt ttttctttta gtttttggaa tttacgggct
    22201 ttatgccgag ttgcttttta aaaagttgag aatgtcggga aagaccaata acctatgtgt
    22261 tgaagccagt tatctgattc aaaaacgtgg gatactaagt aaggcattgt tatttccatt
    22321 tcttaaaata aagtcatcca actcccttat tatttcattt ttcggagcat tagcctgggt
    22381 agtaatagca ttgattattt ttcacagatt ttttaaaagt taactatgaa aaaattctta
    22441 atccttgtct ctctttttac tttcttgatt gtcggttgtc ggaaaactga gaatttaata
    22501 tcttttcatt atttcgataa ggatatttgg aagagatttg attttgttca gtttagcttt
    22561 aacattaaag atactgaaac agaatgggat gtgtggattt tagttgaaca tacaccagct
    22621 tttgaaggcc gatcattacc attaagtatc gaaatgatta ctccatctgg cgattcgcgg
    22681 attctcgatt tggaggcata tttaaggagc acaaaagatg gtaactattt aggggaagca
    22741 aaagaggatt attatgtcgt taaaacattg tcatttcaag ggattcgttt caatgaaaag
    22801 ggtatttgca aatttgaaat agaaaactac aatcataaat actacacgca aggtgttaaa
    22861 accttaggta tcgaatttaa aagagcggaa taattattac tctatctact cttttttctg
    22921 tttttacgcc aacgccgaaa tcttgaaacc aagtcgtaag taattcgtgc agtattgata
    22981 atcagtgagg gattatttac tatgcctgca agaatatcgt ttttgaaatt aatagtatgt
    23041 atttcgttgt ttattttttt aaggctcttt tttatcttct cttctttatg ccgcatctgt
    23101 gtttttagca gcagcttatg atatcggatg tcattaaggt tattgagctt tttcttcttc
    23161 atcatcatcg tgattttcaa tattatccaa aatatcattt atctcttcat cttcaaggat
    23221 catgctattg atccctttga tgaggggatt aagaaaaagt tgtttttgaa ataccaacat
    23281 aaggattact agtgcaagat aaaaaaaagc aataataaca tatccaagga tttcactatc
    23341 agttatacga ttgagccaac ttacaatagc aaaggagata aacaaaatga aaaacaaaag
    23401 caacattgtt gatacaataa ttacaatgaa acgagtaagt attttggata accgttcagt
    23461 taaaataagt gaataatagt ccttttttag ggttaaataa tccttgatgt ttgcaaatgt
    23521 tttattgagt tctttgtcaa tttttttctt atccataaag gcgttattta ttttgtggta
    23581 taagaatgat caagaaagat gaaacgacaa tgaaaaatat tatttcatcc tgtaaagatt
    23641 aaaatcattg ttaacaataa acaaatcagg cttctttttt ctgcattttt cctttcacct
    23701 cttctacttt ttctttcact ttgtttattg attcgccaaa agattgtttc atttcgtcca
    23761 tcttgtccaa gatgtcatct ttcaggtctt cagtcttttg ggcaattttt tttcttgtaa
    23821 ctttaccttt atcaggtgca taaagaattc caagtgcggc gccaatagca gcgccactta
    23881 acaaaccaat aagaaaactt gttgatgatt tcatgattat gtgtttttaa aagattaggc
    23941 aaatatattc aaaaaaaatg ttttctcaat gttttaaaac taaaagttgt taaatgtttc
    24001 ttgtcagatg ccttattatc ttgtactgac taaaaaactc tttagcaata attctaagta
    24061 cggtgtaaga tggtattgca agtatcatac ccggaactcc ggccaaactt cctgcaataa
    24121 gcaggacaac ataaatttcg atagggtgtg ctttaacgct gctcgaataa ataagaggtt
    24181 gaagcacaat gttatctatc agattagcgc ctgcaaaaac tccacataca agaagtataa
    24241 tgccaacaat ttcatgatat tgcccatagc tta
//
LOCUS       DAOOAI010000021        24186 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_2725_length_24186_cov_5.235631, whole genome shotgun sequence.
ACCESSION   DAOOAI010000021 DAOOAI010000000
VERSION     DAOOAI010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24186)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24186)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24186
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2725_length_24186_cov_5.235631"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..807
                     /locus_tag="PK345_02750"
     CDS             <1..807
                     /locus_tag="PK345_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33972.1"
                     /translation="FTFFFNSSFMDFTLKKYKTLLTTLQSAGYAFYTFEHYLSVREHA
                     EKFVILRHDVDLLPKNSLDTARIEASLDIKGSYYFRVVPQSWDEDIIREIAALGHEVG
                     YHYESLTTCNGDIGKAYEDFTANLEKLRRIVAVETICMHGSPRSPWDSKDLWQHYDYR
                     SLGIIGEPYFDIDFNDFFYLTDTGRRWDGYKVSLRDKIPVHQERWISQGLVFRSTKDI
                     IKAANEGRLPDKIMMTFHPQRWNDAFVPWAKELLLQKVKNVVKRGLVLFK"
     gene            1181..2452
                     /locus_tag="PK345_02755"
     CDS             1181..2452
                     /locus_tag="PK345_02755"
                     /inference="COORDINATES: protein motif:HMM:NF025019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX33973.1"
                     /translation="MNRLYLQQILIDQKEAFLNRKGLIQRDMHLDNYLRTKQIVVITG
                     VRRCGKSSLMFLIQQTLQLDESEVCYCNFDDERITSYPALLNDIYALHIEMYQTEPVF
                     FFDEIQLIPGWERFANRMYEQGHKIFVTGSNATLLSSEISTSLTGRNKVIELFPFSFA
                     EYLRFLKKDYALDKLSVKQRSLLLNDLGSYISMGGFPLVVSERDLDIAHALFQDILYR
                     DIVVRFRLTNVEEIREMALFLASNIGKLFSYATLQKITGIKSLSSIKNYLSYFEAGYL
                     FHYLRKFDYSVKKQIMNSRKVYAIDNALPNRLGFGFSGNRGRLLENVVLIELLRRGHE
                     VFYHSNKHECDFLIRENMEIVQAIQVVYELNNENYSREINGLNEAMAAFNIPVGIIIV
                     NFENDYTTKLPPRIDAVPAYKWLLEGDTRKD"
     gene            2789..3718
                     /locus_tag="PK345_02760"
     CDS             2789..3718
                     /locus_tag="PK345_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPX33974.1"
                     /translation="MDIVVENLTKVYGMQRAVDNISFRVNTGEVLGFLGPNGAGKTTT
                     MKAITTYLIPTEGNIWVGEYSIYEQPEEIKKRIGYLPESNPLYQDMPVIDYLKFVGEL
                     QGISSAKIKDRIREMDIVCGLEGEKHKKISELSKGYRQRVGLAQALIHDPDVLILDEP
                     TAGLDPNQIVEIRELIKKIGKQKTVILSSHILAEVEATCDRIMIINKGKIVADGTSDD
                     QRKRAQGKEILKVTIEEADTDTIFEKLKGLPTVELVDILNKAKNSFEVQSKHDDSSRR
                     HIFNLCVKNGWILTELTSLETKLEDVFRELTYN"
     gene            3770..4489
                     /locus_tag="PK345_02765"
     CDS             3770..4489
                     /locus_tag="PK345_02765"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPX33975.1"
                     /translation="MRQIWIITKRELQSFFDSLTAYIMLIAFLGFSGLFTWLYGSDIF
                     FTKQASLQTFFGVSYWTLFFFIPALTMRQFAEENKTGTIELLLTRPVTDWQVIFGKFL
                     ATLLLIAIALVFTLPYYITVAKLGNIDHGATIMGYIGLLFISATYISIGLFASSISNN
                     QIVGFLLALAIGILFHFVFGFLSMASSGFVSEVLYFLSLGAHFESISRGVVDSKDIIY
                     FISIIFLSFVGTEAILQKNRT"
     gene            4514..6031
                     /locus_tag="PK345_02770"
     CDS             4514..6031
                     /locus_tag="PK345_02770"
                     /inference="COORDINATES: protein motif:HMM:NF021351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GldG family protein"
                     /protein_id="HPX33976.1"
                     /translation="MKKSILSQLLLIIVVLILINVLSTRFFVRLDFTGDKIYTLSKAT
                     KDILHSLNEPVTVTAYFTRGSQPEIEKARKDFQDLLIEYSNISKGMVNYEFLNPNEDQ
                     QLEQEVMQSGIQPLVLNVREKDQVKQQRVYLGAKIVMGERTEIIPVVQPGAAMEYTLS
                     SSIKKLSLINKSQIGWIQGHGEPSIHAMQQAVQQLSVLYDLTPVRLEDESVDLHKYLT
                     LAIVAPTDSFSDYELQRLDEYLADGGRLFIAHNHVTVDLQTMQGNVNTGNLSSWLAEK
                     GLTIDNTFAVDRSAGTVGVRQQAGFMTFTRQIPFPYWPAIRNFPEMPITKGLNEVTFQ
                     FASPINFIGAADTSLRFTSILKTSESSGTLSAPLFINLEKQWTERDFPLSGLTLGAIL
                     QGPIAGRGFAKMVLITDGDFAVNGEGQEAQQRPPDNVSLMVNSIDWLSDDTGLIDLRT
                     KEVTSRPLKELEDGKRTFVKWLNFLLPIAFVLVVGVVRVEIRRRKRIKRMEEGYV"
     gene            6036..6995
                     /locus_tag="PK345_02775"
     CDS             6036..6995
                     /locus_tag="PK345_02775"
                     /inference="COORDINATES: protein motif:HMM:NF025598.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HPX33977.1"
                     /translation="MNIKVLVIILVILAAIYAISEFTGNKERSFSRILVAVDTASVSE
                     IHIQIPSDNADIKLVRNALADWSVEANGNRYGADYGVVKSILSQFQEMKPERIAATSK
                     EYWGDFDITDSLAIRVTLSSGKKNVADIYFGKFSYTQPPQGQMQMQQQGKMTTFVRSA
                     KDDRVYAVEGFLRMNYQKDVNSYRNKNLVSVNRDDIARLSFNYPDYSFNVEKVDDRWM
                     INGQPADSLKTLRYLSRIQRLTSVNFVSQDTPKTSDEVYKITVEGNNFSPVELRAIST
                     PDTTISYIITSSANPEGQFDGTKSQLFKLTFVDETEFLPDQPE"
     gene            complement(7117..8073)
                     /locus_tag="PK345_02780"
     CDS             complement(7117..8073)
                     /locus_tag="PK345_02780"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPX33978.1"
                     /translation="MSNPKVLFIDNAHPLLSENLTALGFQCDYFEHFARSDYESVIHN
                     YEGVIIRSKIKIDKSFLQKAENLKFIGRVGSGMENIDVSFAESKGIRCINAPEGNRSA
                     VGEHTLGLLLMLLNNLAKADKEVRQGLWIRKGNTGIEVEGKTIGIIGYGNMGSAFAKR
                     LSGFGAKVIAYDKYKFGYADQYVKEAELEELFKYADIVSMHVPLTPETEYMVDEKFID
                     SFQKPIYLINTARGKVVNTEALVKHLKSGKIKGAALDVLEYENYSFENIENQNIPNAF
                     KYLIEADNVVLSPHIAGWSHESAQKMAEVIVEKIKAIYSSNL"
     gene            complement(8084..9271)
                     /locus_tag="PK345_02785"
     CDS             complement(8084..9271)
                     /locus_tag="PK345_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33979.1"
                     /translation="MNKIISLVILVVLVSCSGTNTLIKQNRYDEAIDLLTKQVTKQTF
                     SIKTIEKIDQIMNEAVKKDLSTIEHLKLSGEPDVWYEIFGIYQKIEARQQKISTLPDT
                     AINLMQYKIEDYSDYTNQARIKATQYHWAKAERHLENNDPKEIEKAYHHLLKVQELTP
                     GYKTSNELLSNFKKARPVEIFYRVNNRFKGYLPPAVIDEITYLDLSSLNTTTYKFRNK
                     KTKKQPFDYIISIDIYDVKIIPENTNDSYYVETAQVQDGIAYKLDDNANFVYDSLGRK
                     IEYPKLKNIACYVTETVKKKAIFIGGNVIISEAATGKTIGEQAISGESRFLSRSAKFK
                     GDINALAPETRELLGSKEAKYPSDLELIMRASDKFKINAANYIIQEIKQKSPITTEAD
                     HSF"
     gene            9428..11839
                     /locus_tag="PK345_02790"
     CDS             9428..11839
                     /locus_tag="PK345_02790"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPX33980.1"
                     /translation="MWTYLARLILRRRNWNLGVILILTIFMAYKARQIGLSYEMTQML
                     PASDTTRVYYEKFKQIFGEDGSVIFIGIDDENIKNLDEFNDWYDLTSEIQNISGVEGV
                     LSVTRLFNLIKNDSLYRFDIKPVIQRKPRTQVELDSLLNIAYGLPFYEGLLYNKETNF
                     TMMAVTLDKEVLNTKQRVGMIYAIKDAGERFSEKHNTPIHFSGLPYIRTITAKKIESE
                     LLFFVGISLLIASTILMIFFRNWKMVATTMLIVIINVIWLMGFIVLFGYKITAVIGIL
                     PPLLIVIVVENSIFLLNKYHYEILRHGNKIKALTRVVMYIGNANLLTNATTATGFAAF
                     IVTGNQVLIEFGTVASISILSAYLMTLFLIPILFSFLPPPSVNHTVHLEQGLVRRIVE
                     RVVSIVQCHRRVIYIVTMLIVLAGIYGITLLKTTGNIVDDIPKKDRLYRDLVFFEKNI
                     KGVMPLEFSIDTKKKRGVMQLATLQKIDQLQHVFEDYPELSKALSVVEVVKFAKQAFY
                     GGNPERYDLPNNQERNFIFRYVPDMKTDQRTIINSFVDTSLQLARVTVQMANIGTKDI
                     QRIKDDLTPRIDSIFPSEQYRVDMTGTSVVFLKGTNYLISNLIWSLFLAMIVITILMS
                     LLFSSLRMIMISMVPNLIPQIMTAAMMGFLSISVKPSTILIFSISLGISVDNAIHFLS
                     RYRLHLRLNKWEIKTSVINALRETGFSMIYSSSVLFFGFIIFTTSSFGGTEALGYLIA
                     FTMLVALLCNLFVLPSLLLSLDKKITTRNFSEPYLEIFDEEIDIELDDLKVEIADVSE
                     LKKEI"
     gene            11861..12724
                     /gene="rfbA"
                     /locus_tag="PK345_02795"
     CDS             11861..12724
                     /gene="rfbA"
                     /locus_tag="PK345_02795"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HPX33981.1"
                     /translation="MKGIILAGGAGTRLHPITMAISKQLMPIYDKPMVYYPLSILMMA
                     QIRDILIISTPEDLPNFEKLLGDGSRIGCNFSYKVQHVPNGIAQAFVLGEEFIGKEKA
                     ALILGDNIFYGTGLQALLQENNDPDGGVVFAYHVSDPERYGVVEFDKTNRAVSIEEKP
                     KNPKSNYAVPGLYFYDNSVVEVAKNIKPSARGEYEITDVNRHYLEQGKLKVGILSRGT
                     AWLDTGTFESLLQASQFVEVIEHRQGLKIGCIEEVAYRMGFINAEHLEKIAQPLLKSG
                     YGEYLIRLIKS"
     gene            12806..13783
                     /locus_tag="PK345_02800"
     CDS             12806..13783
                     /locus_tag="PK345_02800"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HPX33982.1"
                     /translation="MKEYEYFLNLAESAISKIDLPELPSRLYDPIRYILGMKGKRIRP
                     VLTLVSCDLFGGHADDAIDAAIGLEIFHNFTLLHDDIMDEAMLRRGKDTVNKKWNINI
                     AILSGDTMFALACRYMALRCSVRREEIFDVFTKTAIEVCEGQQLDMDFETETEITIDR
                     YFEMISLKTAVLLGACTKIGALCANAASEQADMLYDFGKNAGMAFQVQDDLLDAFGNT
                     DKFGKNVGGDIVTNKKTFLYLKCLEVANDHDKERLKSMFSGTETIDPEVKISQVIDIY
                     NKYNIKQLAMKQVESFFNQATDIMQMVNGDPSKKMLFLNYVEWLYKRDF"
     gene            complement(13806..16292)
                     /locus_tag="PK345_02805"
     CDS             complement(13806..16292)
                     /locus_tag="PK345_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HPX33983.1"
                     /translation="MSIPKHTMKPFSLLISLLLLIFLTPFDFVYAQTKKSQQADEDFN
                     NLRFSVAIPKYKKAYARSKRNKLERNRITYQMGECYRLTNQPKRAAAFYKRLTRTGYD
                     RIEPLVLLRLADMQRANGNYEEALSNYETFLERVAGDPLGLVGMQSCKLAIEWEKNPS
                     NFKIETIKKINSRENDFSPSYSDPTGRVIIFTTSRDGVVGKKPDEWTGLNFTDLFTTK
                     QDAKGNWSQPVTIDDTEALNTEDNEGQAAFNENFSRMYFTRCSRDNLTENGCQIWVTS
                     KQGRSWSEPIMVDLGGDSTSVFGHPAISPNETTLIFSSSRKGGAGGRDLWIATRNSAS
                     ENFTALRNLGELINTPGDELFPTLRGDSVLYFASDGHPGMGGLDIFKSVKQENGTWGE
                     PVNMKPPINTNADDFGITWHPEGNNEGFFSSNRQGGRGGDDIYYFISPPIIYNIRGKV
                     LDKNTLEPIEEALISLVSSDGTTVQVKSNSVGYYEFIDGQVKPGITFELITEKPKYFI
                     EKAMETTLGVDASKDFEINFELEPIPAEPILLPEILYDLAKWDLKPQFQDSLQGLIRT
                     LDANETLVIELASHTDARGSLESNDILSQRRAESVVNYLIQRGIDPERLIAKGYGERA
                     PRKLKTDITRDGFNFTAGTILTESYIDSLPTLPMREAAHQLNRRTEFSILRNDFIPKP
                     KIRIEHDSVDIKIITEPEKNIIPFTKTPTGAIEATCIVNGIKLNFIYDRKTLRPTISL
                     GSAMRLLKDGAITRADFQGNPDNIFKDGSIANRSLFTVKSFMIGNEKLEKIEFQVDHK
                     LTSQLMLGEITLLMVGEYEIDEENMQIVFK"
     gene            complement(16367..16774)
                     /locus_tag="PK345_02810"
     CDS             complement(16367..16774)
                     /locus_tag="PK345_02810"
                     /inference="COORDINATES: protein motif:HMM:NF014470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HPX33984.1"
                     /translation="MTLLFFNIGGGELFLIVLVIFLIFGPEKIPEIARFFGKGINEIK
                     KATNQIRDEIEKETGDITNVATNIKNDITEGLKQISSLDTEYNKKTYDSDYREDINKL
                     QNKEANKNEAEPDAEQKKESDQKTSLPDSMPVE"
     gene            complement(16870..20025)
                     /gene="secDF"
                     /locus_tag="PK345_02815"
     CDS             complement(16870..20025)
                     /gene="secDF"
                     /locus_tag="PK345_02815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecDF"
                     /protein_id="HPX33985.1"
                     /translation="MQNKGFIKGIAILFALACLFHLSFTFVTARVEKKARAFAYGQET
                     EMQANALAGDDLTKYQFLYDSISRSKEVYYLDSMSNQIVYNILIRKYTYKEVKERELN
                     LGLDLKGGMNVTLQVAVSDIIVALSGHSQNPVFVEAIERAKLKERNSQEDFVALFGQA
                     INEIDPNFQLASLFMAEFKDKINFNSTNEDVLRILSTETNQAIDRTFNILHSRIDRFG
                     VTQPNIQKLQTAGRILVELPGIKEPDRVRKLLQGTAQLEFWETYKFPDLYNYFEAADS
                     KLVDILKLESEAESKEEADSNEEIITDEELAETTEAAEETIEDVEITEEADTTQSKLL
                     DEIGDATSDTQTMSSEELAKTNPLFAYLRPNLQQTQGGGWAPAPNATVGFAQIKDTAR
                     INNYLRKINKDGDAFPRNLKLAWKVKPEKWAPDLLELVALKVTSRDGTPALDGGVIVD
                     ARQDFEPTSGRVEVTMLMNSEGARIWKQLTGDNIGNQIAIVLDDYVYSAPNVNDEIPS
                     GRSSITGNFEVAEAQDLANILKAGKLPAQAHIVEEAIVGPSLGRIAVTNGLISFVLAF
                     IMVLAYMALYYNKAGLVANLALLTNILFIFGVLASLGAVLTLSGIAGIVLTLGMAVDA
                     NVIIFERIKEEIRAGKGAKLALSDGYKNSYSAIIDGNVTTLLTGIVLYIFGSGPVQGF
                     ATTLIIGIVSSLFSAIFISRLVFDYLLSRNKSIAFGNSLTINVLSTVNINFLGMRKKA
                     YILSAAIIFIGLVSLATRGLNYGVDFSGGRTYVVRFDQPVNTNEIREALVEPFEQQPE
                     VKTFGPTSQVKITTKYNINDNSPAMDSIIQRKLFESLNQFYNDKSISYDEFVTVTDSE
                     KLTGILSSQKVGPTIASDIRNRAVLSVVFALIIMFIYIAIRFKRWQFGLSGVIALFHN
                     AMITISLFSLFYNVLPFNLEIDQAFIAAILTIIGYSINDTVIIFDRIREHFSLYPKRT
                     LMTNINDGINSTLSRTINTGGTTLVVLLMIFIFGGEVIRGFAFALFVGILIGTYSSIF
                     LASTLAYDLLSRRKVKVKTKA"
     gene            complement(20214..22175)
                     /locus_tag="PK345_02820"
     CDS             complement(20214..22175)
                     /locus_tag="PK345_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005858801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPX33986.1"
                     /translation="MNSKNDFKVIAEEFEDYRILRYQVTGFENLPLKKKILLYYLYEA
                     ALSGRDIIWDQNYRYNLLIRKTLEHILKYSQKLKEDENWNLFLAYLKKIWFSNGIHHH
                     YSMEKIVPHFSENYFTSLLTDTPKPDFLKDNENIEDFIHWITPLLFDPEIAAKRVVLD
                     KGVDLLAASANNFYENVNQKEAEEFYASMENPDPLRPLSFGLNSKLVKVDGKVEERVW
                     RVGGMYSAAIERIVFWLEKSIEVAETNGQKAALEKLVEFYKTGNLQTFDEYNILWLND
                     TESDVDVINGFIEVYGDALGRKATYESVVQLHDFEGTHRAEIISTNAEWFEMHSPILE
                     EYKKKSISGVSARSINVVVASGDCSPSMPIGINLPNADWIRAEHGSKSVTLANIIDAH
                     TEANKESGIIEEFYWSEYEIELSRKWNKLATQLHVDLHEIIGHGSGRLKEGVAQPHET
                     LKNYASTIEEARADLFALYFAIDPKLVELGLAPSIDVGYAEYNSYITGGLITQLARVE
                     MGKNLEESHMRNRQLIAKWAYEQGFKKNIIEKKRRDGKTFFVINNHDELREIFGKLLH
                     EVQRIKSEGDFRAAKYLIEKYGVKVDRKLHQEALQRWKSLGIPKFSGFINPVLQPIKK
                     NGKILDISMHYPENFSDQMLFYAKNYSFL"
     gene            complement(22206..23723)
                     /locus_tag="PK345_02825"
     CDS             complement(22206..23723)
                     /locus_tag="PK345_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HPX33987.1"
                     /translation="MKILPVELIREADAYTIKNEPIASIDLMERAAYQCFKWIKKVVR
                     RDAPINIFCGPGNNGGDGLVISRLLIADGYHVVTIILRISNKFSPDFITNLERLEKSK
                     KAVIYNVTTLQDLPDIPDGSIIIDAIFGSGLSKPIEGFAAEVIRLINDTNSTRIAIDI
                     PSGLYADQHSALPKAAIVKADYTLTFQYPKLSFLFAENEHYVGKWIVLPIGLMDEATK
                     DKEIRNFTIDRADAFSLLKSRTQFSHKGHFGHALLIAGSYGKTGAAVLAAQAALRSGA
                     GLVTAHLPASSRQIMQTAAPEVMVSIDNNDRFISHHPDLSLFNTIAIGPGLGLAKETQ
                     QALKLLIQNATVPLLFDADALNILAENKTWISFIPPLSIFTPHPKEFERLFGKFANAF
                     ERNKRQIEFSVMHRIYIVLKGAFTSISTPDGKCFFNTTGNPGMATGGSGDVLTGVILG
                     LLAQGYHPQEAAILGVYLHGLAGDIASKDKSQEAMIAGDITENLGAAFKKIRKNK"
     gene            23878..24057
                     /locus_tag="PK345_02830"
     CDS             23878..24057
                     /locus_tag="PK345_02830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33988.1"
                     /translation="MFTFEADFKNYTDRISQIDELLIKRLSTLALVCESIVLLRNFLK
                     ESLQLSGKKHKKSDD"
BASE COUNT         6853 a         4597 c         5087 g         7649 t
ORIGIN      
        1 tttacctttt tcttcaactc ttcatttatg gacttcacac ttaaaaaata caaaacttta
       61 ttaacaaccc ttcaatctgc aggttatgct ttttacactt tcgagcacta cctgagtgtg
      121 cgcgagcatg ccgaaaaatt cgtaattcta cgccacgatg ttgatctcct accgaaaaat
      181 agtctggata cagccaggat tgaagcttca ttagacatta aaggttccta ttatttcagg
      241 gttgttcccc aaagctggga cgaagacata atccgcgaga ttgccgccct tggccatgag
      301 gtgggctatc attacgaatc gctgaccacc tgcaacggcg atattggaaa agcttacgaa
      361 gatttcactg ccaatttgga gaagcttcgc cgtatagttg ccgttgaaac catctgtatg
      421 cacggtagtc cacgctcccc ttgggatagc aaagatttgt ggcaacacta tgattaccgt
      481 tcccttggca tcatcggtga gccttacttc gacattgact tcaacgattt cttttacctt
      541 actgataccg gccgccgctg ggatggttat aaggtgagcc tacgagataa gatacccgtt
      601 catcaggaac gctggatatc tcagggatta gtcttccgga gtactaaaga tatcatcaaa
      661 gccgccaacg aaggtcgcct gcccgacaag atcatgatga catttcaccc gcaacgctgg
      721 aacgacgcct ttgttccctg ggcaaaagag ttgctattac agaaagtgaa gaatgtggtt
      781 aagagggggc ttgttttgtt taaataggtg ggagagtggg caaattgttg ttatagttta
      841 aagtttcaca gagttaggca cagagtactc tgtttagttt tctctgcatt actctgtgca
      901 actttttatt ttcgcaatgg tttgtacgta gttttatagg catcggctat gatggagagc
      961 gggttgggaa tagctgttag ccgctgtttc cgtttattta cccgaaacta tgatcttgat
     1021 attattggat agattaacca accctgcaat taacatcaga tgtttaatat agtagagata
     1081 tcaaaaatca ttctttataa acgaataata ttagtaaaat atgttcgttt ctaatgaaaa
     1141 gaaattatct ttgccaatcg taaatatagc gacatagtaa atgaacaggt tgtacttaca
     1201 acagatactt attgatcaga aagaggcatt tctaaaccgg aaaggcttaa ttcaacggga
     1261 tatgcatctt gataattatc ttcgcacgaa gcagattgtt gtaattaccg gagttcgtcg
     1321 ctgcggtaaa tcttctttga tgtttttgat acagcagact ctgcagcttg atgaatcgga
     1381 ggtctgttat tgtaatttcg atgatgagcg tattacctct tatccagcgc tgctcaatga
     1441 tatttatgct ttacacattg agatgtatca aacagaacct gttttctttt tcgatgagat
     1501 ccaacttatt ccgggatggg agaggtttgc caacagaatg tatgagcagg gccataaaat
     1561 ttttgtaact ggctccaatg ccactttgct aagttcggag atatcaacat ctttgacagg
     1621 acgaaacaag gtgattgaac tttttccatt ctcatttgcc gaataccttc gttttctaaa
     1681 aaaggattac gcattggata aactttcggt taagcaacgt tcactgcttt taaatgacct
     1741 cggttcgtat atctctatgg gtggatttcc gttagtagtt tcggaacgcg atttagatat
     1801 tgcccatgcc ctgtttcagg atatactcta ccgcgacatt gtggttcgct ttcgcctgac
     1861 caacgttgag gagattcgtg aaatggcttt gtttttggca tcaaatattg gaaagctttt
     1921 ttcatatgct actcttcaaa aaattacagg tattaaaagc ctgagctcca taaaaaatta
     1981 tctctcctat tttgaggcag gatacctctt tcattatttg cgaaaattcg attattcggt
     2041 caaaaagcaa atcatgaatt cgcgtaaggt gtatgcaata gacaatgccc tgccaaatcg
     2101 tttgggtttt ggtttttccg gaaatagggg tcgtttgttg gaaaatgttg ttcttatcga
     2161 gttacttcgt agagggcatg aggtttttta tcattcaaac aagcatgaat gcgatttttt
     2221 gattagggag aatatggaaa ttgtacaggc aattcaagtt gtttacgaat tgaacaatga
     2281 aaactatagc cgtgagataa acggactaaa tgaagctatg gctgcattta atattcctgt
     2341 aggtatcata attgtgaact ttgagaatga ctatactact aaattgccgc cgagaataga
     2401 tgctgtgccg gcatataaat ggttattgga gggcgataca agaaaggatt agatagagta
     2461 tgttttggac tttattctga aatcatacca ccttttctgt gatgtttttt tgttgcacag
     2521 agttgcacgg agtttcactg agtactctgt ttagttttct ctgcattact ctgtgtaact
     2581 tttctttttc accgaatttt ttatttctta aaacaaccta aaatcacaat cattgatcct
     2641 gttttgtttg gctttaacaa taagtatgat catatttatt aagggatata gttatattac
     2701 cgttaaataa tgttaaccaa aaaaatagtt cctcaataaa ctactctatt tttagttact
     2761 ttgcatatga attttaaaag agaagaatat ggacatagtc gttgaaaatt tgactaaggt
     2821 ttatggtatg caacgcgcgg ttgataatat ctctttccgc gtaaacaccg gggaggtgct
     2881 tggcttttta ggtcctaacg gagctggaaa gaccactacc atgaaggcta ttaccaccta
     2941 tcttatccct actgaaggta acatttgggt gggggagtac tctatttatg agcagcctga
     3001 agagatcaaa aagcgaattg gctacctgcc cgagagtaac cctctttatc aggatatgcc
     3061 cgttattgat tacttaaagt ttgtaggaga gttgcaggga atcagttcgg cgaagatcaa
     3121 agataggatc agggagatgg atatcgtttg cgggcttgag ggcgagaagc ataagaagat
     3181 cagtgagtta tccaaaggtt atcgccagcg tgtcggtctt gctcaggctt tgatacacga
     3241 ccccgatgtg cttatcttag atgagcctac tgcaggtctt gaccctaacc agatcgttga
     3301 gatcagggag ctgataaaga agatagggaa acagaagaca gtgatcctta gctctcatat
     3361 ccttgccgaa gtagaggcaa cctgcgatag gataatgatc attaacaaag gtaaaatcgt
     3421 tgctgatggc acctccgacg accagcgcaa aagggcgcaa gggaaagaga tattaaaagt
     3481 aaccattgaa gaggccgata cagacactat ttttgaaaaa cttaaggggt tgcctacagt
     3541 tgagttggtg gatatattaa acaaggccaa aaacagtttt gaggtacaaa gcaaacacga
     3601 cgactcctca aggcggcata ttttcaacct ttgtgtgaag aatggctgga tacttaccga
     3661 gcttacatct ttagagacta aacttgaaga tgtcttccgc gagcttactt acaattaatc
     3721 tcttcaacag tataataatc attaaaatat aaaaaaatag tattaagata tgaggcaaat
     3781 ttggatcatc actaaaaggg aattgcagtc cttctttgac tcgctcacag cctatatcat
     3841 gctgattgcc tttcttggat ttagcgggtt atttacatgg ctctacggtt ccgacatctt
     3901 ttttactaag caggcaagcc tgcagacctt ctttggcgtt tcttactgga cattgttctt
     3961 ttttatcccg gcgctcacca tgcgtcagtt tgctgaggag aacaaaacag gaactattga
     4021 actgctcctt acccgtccgg taaccgactg gcaggttatt ttcgggaaat tcttagcaac
     4081 cctattactc attgctattg cactcgtgtt tacattgcct tattacatca ccgttgcaaa
     4141 gttgggtaat attgatcatg gcgctacgat aatggggtat attggcctgc tgtttattag
     4201 tgcgacttat atcagcatag gtctttttgc aagtagtatt tccaataatc agatcgttgg
     4261 ttttctgtta gcgcttgcga ttgggatact ctttcatttt gtatttggct ttctatcaat
     4321 ggcttcgagt ggctttgtca gcgaggtgct ctatttcctg agccttggcg ctcatttcga
     4381 atctatctcg cgcggggtgg tggatagtaa ggatattatc tactttatct caataatctt
     4441 tttgagcttt gttggtacag aggctatatt gcaaaaaaat agaacttaag aaaggaggaa
     4501 attaagtatg aaaatgaaaa aatctatact gtcgcaactg ctgcttatta tagttgtgct
     4561 aatcttgatc aatgtacttt ctacacgttt tttcgtccgt ctcgatttta ccggagataa
     4621 gatttacacg ttaagcaaag ctacaaagga cattttacat tcgcttaacg agcctgtaac
     4681 agttacggcc tattttaccc gtggttctca gcctgaaata gaaaaggcgc gaaaggactt
     4741 tcaggatttg ttgattgaat attccaatat ttccaaaggg atggtgaatt atgagttcct
     4801 caatccgaat gaagaccagc agttggaaca agaggtcatg cagtcgggta ttcagccgtt
     4861 agttttgaat gtaagggaga aggatcaggt gaagcagcaa agggtttatc tgggggcaaa
     4921 gattgtgatg ggcgaacgca ctgaaataat ccctgttgta caacctggtg cagctatgga
     4981 gtacacgtta tcatcgtcaa taaaaaagct ttcgctgatc aataaatctc aaattggctg
     5041 gatacaaggt catggcgaac cctcgataca tgcgatgcaa caggcagtgc agcagttaag
     5101 tgtactttat gacttgacgc cggtgaggct tgaagatgag tctgttgatt tacataaata
     5161 ccttactctc gctatagtgg cgccaacaga ttcgttttct gattatgaat tgcaaagatt
     5221 ggatgagtat ttggctgatg gcggacgttt gtttatcgca cataatcatg taactgttga
     5281 tctacagact atgcagggca acgtcaatac gggtaatctt agcagttggt tggccgaaaa
     5341 agggcttacc attgacaata cctttgctgt ggataggagc gccggcaccg taggcgtgag
     5401 gcagcaggca ggttttatga ccttcactcg tcagatacct tttccttact ggcccgctat
     5461 ccgaaatttt cctgagatgc ccattacaaa aggacttaat gaggtaacgt ttcaatttgc
     5521 aagccctata aattttattg gcgctgcaga tacatcactt aggtttacat caattttaaa
     5581 gacttcggaa agttcaggaa ccttgtcggc gcctttattt atcaatcttg aaaaacagtg
     5641 gacagaaaga gattttcccc tttccggatt aacacttgga gctatcttgc aaggacctat
     5701 tgctgggaga ggttttgcaa aaatggtgtt gatcaccgat ggcgattttg ccgtaaacgg
     5761 tgaaggacaa gaggcacaac aaaggccgcc tgacaatgta agcctgatgg taaactccat
     5821 tgattggctt tcagatgata ccggcctgat tgatctacgt actaaagagg ttacttcaag
     5881 gccgttgaag gaattggaag atggaaagag gacatttgtt aaatggctca acttcctctt
     5941 accaattgct tttgtactgg tagtgggtgt tgttagagta gagatcagaa gaagaaaacg
     6001 tataaagaga atggaggaag gttatgttta aaaaattgaa tataaaagta ttagtcatta
     6061 ttttagtaat attggcggca atttatgcaa ttagcgagtt taccggaaat aaggaacgct
     6121 cgttcagcag gatcttagta gctgttgata ctgcttcggt aagtgaaata catattcaaa
     6181 ttccgtctga taatgctgat attaaacttg ttcgcaatgc attagccgat tggagtgtgg
     6241 aggctaatgg gaatcgctat ggcgctgatt atggcgtggt gaaatctatc ctatcacagt
     6301 ttcaggagat gaagcccgaa cgaattgcag caacatcaaa agagtactgg ggtgattttg
     6361 atatcaccga ttcattagct attcgtgtaa cattgagcag cggtaagaaa aatgttgccg
     6421 atatttattt cggaaaattt tcttacaccc aaccgccgca aggtcagatg cagatgcagc
     6481 agcagggaaa aatgaccact ttcgtacgta gcgccaaaga tgatagggta tatgcggtag
     6541 aaggattttt gcggatgaac tatcagaaag atgtaaactc gtatcgaaac aagaatttgg
     6601 ttagcgtcaa cagggatgat attgcaaggt tgagttttaa ttatcctgat tatagtttta
     6661 atgttgaaaa ggtggatgac cgttggatga ttaatggtca gcctgccgac tcgctgaaga
     6721 ctttacgtta tctgagcaga atacagcggc ttaccagcgt caatttcgtt tcgcaggata
     6781 cgccaaaaac cagcgatgag gtttacaaaa ttacggttga aggaaataat ttttcgccgg
     6841 tggaacttag ggctatatct actcctgata ctaccattag ttacatcata acatcgtcag
     6901 ctaatcctga aggtcaattt gatggaacta aatcgcaact ttttaagctg acctttgtgg
     6961 atgaaacaga atttttaccg gatcagccgg aatagatatt attgaaaaaa aaacgaaacc
     7021 ggagggctaa tgaagttgct ttccggtttt tttttgcctc aagatcaggt tggcggtagg
     7081 cagaccaaag gttattggtc aatttgggat tcggagttat aagttggatg aatagattgc
     7141 tttaattttt tcaacgatca cttcagccat tttctgcgcc gattcgtgcg accagcctgc
     7201 aatatgcggc gacagaacca cattatcagc ttcaataaga tatttaaaag cgttcgggat
     7261 attttgattt tcaatgtttt cgaaagaata attctcatac tccaaaacat ccaaagcggc
     7321 gccctttatc ttacctgatt tcaaatgttt taccaacgct tcggtgttca ctacctttcc
     7381 tctggcggtg ttgataaggt aaattggttt ttggaagcta tcaataaatt tctcgtcaac
     7441 catatattcg gtttcgggag ttaaaggaac atgcatgctt acgatgtcag catatttaaa
     7501 aagctcttcg agttcggcct cttttacata ttggtcggcg tatccgaatt tatatttatc
     7561 ataagcgatc acctttgctc caaatccgct caatcgtttg gcaaaggcgc tgcccatatt
     7621 tccatagcca atgatcccta tcgttttacc ttctacttct attcctgtgt ttccttttct
     7681 tatccaaagt ccttgtcgaa cttccttatc tgcttttgca aggttgttca gcagcattag
     7741 caacagcccg agtgtatgtt cgcctactgc gcttcggttg ccttcaggag cattgatgca
     7801 cctaatcccc ttagattcgg caaagctgac atcaatattt tccatgcccg agcctacccg
     7861 gccaataaat ttcagatttt cagccttttg tagaaacgac ttatcaatct tgattttact
     7921 gcgtatgata acgccttcgt aattgtgaat taccgattca taatctgatc tggcaaaatg
     7981 ttcgaaatag tcgcactgaa aacccagagc ggtaagattt tcacttagca gcgggtgtgc
     8041 attatcaata aagaggactt tgggattcga cataaaaaag aggttaaaaa gagtgatctg
     8101 cttcggttgt aattgggctt ttctgtttta tctcttgaat gatgtaattc gcagcattaa
     8161 tcttaaattt atcgctggcg cgcattatca gttcgaggtc ggaggggtat tttgcctctt
     8221 tgctaccaag aagctcccgt gtttcaggag ctaatgcgtt gatatctcct ttaaactttg
     8281 cggaacggct gaggaatcgg ctttcgccgg aaatagcctg ttcgccgatg gttttaccgg
     8341 tagcagcctc agatataatt acatttccac cgataaaaat cgcctttttc tttactgttt
     8401 cggttacata gcaggcaata tttttaagtt taggatattc aattttccgg ccaaggctat
     8461 cgtaaacaaa gttggcgttg tcgtctaact tgtaagctat tccatcttgt acctgggctg
     8521 tttcaacata atatgagtcg ttggtgtttt ccggaatgat tttcacgtca taaatatcta
     8581 tcgagatgat gtaatcaaat ggctgtttct ttgtcttctt gtttcggaat ttataagttg
     8641 tcgtatttaa agaggagagg tcaagatagg tgatctcatc aattacggcg ggaggcagat
     8701 agcctttgaa gcggttattt acccgataaa atatctcaac aggccttgcc ttcttgaaat
     8761 tcgacagaag ctcgttgctg gttttgtatc cgggggtgag ctcctggact tttaaaagat
     8821 gatggtacgc tttttcgatc tcctttggat cattgttttc taaatgcctc tcggctttag
     8881 cccaatgata ttgcgttgcc ttgatgcgtg cctgattagt ataatctgag taatcttcga
     8941 ttttgtattg catcaaattt attgcagtgt cgggcaaagt tgatattttt tgctgacggg
     9001 cttcaatttt ttgataaatc ccaaagatct cgtaccacac atctggttct cctgatagct
     9061 taagatgctc gatggttgat agatcttttt tgacggcttc gttcattatc tggtcaattt
     9121 tttcgatggt tttgatgctg aatgtttgtt ttgttacttg ctttgtaagt aaatcaatag
     9181 cttcatcgta gcggttttgc ttgattaaag tgtttgtgcc tgagcacgac acaaggacga
     9241 caagtattac taaactaatt atcttgttca tggcatttag aataattatt tattaaacga
     9301 gaattatgga aatatcttta gttatctaat ctgaaatcaa ttaaaaacgt aatttcgagg
     9361 cctgattttt tatttaataa attaaggcat atctaaacaa ttttagtacc aaaactattt
     9421 aaatgaaatg tggacgtacc ttgccagact gattttgaga aggaggaact ggaaccttgg
     9481 cgttattctt atccttacaa tattcatggc ttacaaagcg cgacagattg gtctctcgta
     9541 tgagatgacc caaatgttgc ctgcctccga taccacaaga gtatattatg agaagtttaa
     9601 gcaaatcttt ggagaagatg gaagtgtaat ttttatcggc attgatgatg aaaacattaa
     9661 aaatctggat gagtttaacg attggtatga tctcacaagt gagattcaaa atatatcggg
     9721 agtcgaaggt gtgctctcgg ttacccggtt gttcaatttg ataaaaaacg attcgcttta
     9781 ccggttcgat atcaagccgg taattcagcg taaacctcgc acacaagttg agttggacag
     9841 cttattaaat attgcttacg gtcttccatt ttacgaaggt ctgctctata ataaagagac
     9901 gaattttaca atgatggccg taacgctcga caaggaggtg ttaaatacca aacaacgtgt
     9961 cggtatgatt tacgctataa aagatgcagg cgagcgtttt tctgagaagc ataatactcc
    10021 gatacatttt tctggtttgc cctatatccg gactattacc gcaaaaaaaa ttgagagtga
    10081 gctattgttc tttgtcggca tatcattatt gattgcatcc accattttga tgatcttttt
    10141 ccgtaattgg aaaatggtag ctaccaccat gttgatagtt atcattaatg tgatctggct
    10201 gatgggattt atagtcctgt tcgggtataa gattacggcg gtaataggta ttctgccgcc
    10261 gctactaatt gtaatagtgg tggagaattc aattttcctg ctcaacaaat atcattacga
    10321 gatactacgt catggcaata agattaaagc tctgacgcgt gtggttatgt atatcggaaa
    10381 tgccaactta ctcaccaatg cgacgactgc aaccggattt gccgctttta tcgtaacagg
    10441 taatcaggtt cttattgagt ttggaacagt agcatcaatt agtatattgt cagcctatct
    10501 gatgacactt ttcctgatcc cgatactatt cagcttttta ccaccgccat ctgttaatca
    10561 taccgtgcat ttagagcaag gccttgtaag aaggatcgta gaaagggtgg tatcaatagt
    10621 acagtgccac cgcagggtga tctatattgt tacaatgtta attgttttgg ctggcattta
    10681 tggcatcact ttacttaaaa caaccggaaa tattgtggat gatattccta aaaaagatag
    10741 actgtaccgt gatcttgtgt tttttgagaa gaacattaaa ggggtgatgc ctttagagtt
    10801 ttctattgat acaaagaaaa aaaggggagt tatgcaattg gctactctcc aaaaaattga
    10861 ccagttgcaa catgtgttcg aggattatcc cgagttgtcc aaagcgctct cagtagtgga
    10921 ggtggtgaaa tttgcaaaac aggcctttta tggcggtaac cctgaaagat atgacctacc
    10981 aaataatcag gagagaaatt ttatctttag atatgtgccc gatatgaaaa ccgatcagcg
    11041 aacaattata aactcgtttg ttgataccag ccttcagttg gcgagggtta ccgtgcagat
    11101 ggccaacatt ggaactaaag atatacagcg gataaaagat gacttaacgc cacgtattga
    11161 tagcatcttt ccatcagaac aatatagagt ggatatgaca ggtaccagcg ttgtttttct
    11221 taaaggaacg aactatctta tcagcaattt gatctggagt ctttttctgg ctatgattgt
    11281 tatcacgata ttgatgtcgt tgctcttttc ttcacttcgt atgattatga tatcaatggt
    11341 tcctaacctt ataccacaaa taatgacagc agccatgatg ggctttctct ccatttccgt
    11401 aaagccatca actatactga tctttagtat ttcacttggg atttcggtgg ataatgccat
    11461 tcattttctg tcgcgctacc gtctgcactt acgcctgaac aaatgggaga taaaaacttc
    11521 ggtaatcaat gcgttgagag aaacaggttt tagtatgatc tattcgtcat cggtactctt
    11581 ttttggcttc attatcttta ccacatcgtc gtttgggggt acggaggcgt tagggtacct
    11641 tattgccttt acgatgctcg tagcgctact ttgcaaccta tttgtcctgc cttcgttact
    11701 gctttctctt gataagaaaa tcacaacgcg caatttttct gaaccctatc tcgaaatatt
    11761 tgatgaagag attgacatcg aattagatga tctaaaagtt gaaatagccg atgttagtga
    11821 gttgaaaaaa gaaatttaac catttataaa aatattaatt atgaaaggaa taatattagc
    11881 aggaggcgcc ggcacaaggc ttcatcctat taccatggca ataagtaaac aattaatgcc
    11941 aatctatgat aaaccgatgg tttattatcc gttatcaata cttatgatgg cacaaataag
    12001 ggatatactg atcatttcaa cccctgagga tttgccaaac tttgaaaaat tactcggcga
    12061 tggatcgcgg attggatgca actttagcta caaggtacag cacgttccaa atggaattgc
    12121 tcaggcattt gtgcttggcg aagagtttat cggaaaagag aaggcagcat tgattttggg
    12181 cgataatatt ttttatggaa ccgggctgca agctctgtta caggaaaata atgaccccga
    12241 tggcggagtt gtatttgctt accatgtttc ggatcctgaa cggtatgggg tagtggagtt
    12301 tgataagaca aaccgggctg tttcgataga ggagaaacct aaaaatccga aatccaatta
    12361 cgctgtgcca ggtttatatt tttatgataa cagcgtggtt gaagtggcca aaaacataaa
    12421 acccagcgca cgtggagaat atgagattac tgatgtaaac cgccattatt tagaacaagg
    12481 gaagctgaaa gtgggaatct tgagtagggg tacagcatgg ttagataccg ggacttttga
    12541 gtcgttgttg caagcttccc agtttgtcga agtgatagaa catcgtcagg gactgaagat
    12601 cggatgtatc gaggaagttg cctaccgaat gggctttatt aatgccgaac atcttgaaaa
    12661 gatcgctcag cctttgctga aaagtggcta tggcgaatat ttgataaggc tgatcaaaag
    12721 ttaaagtaag agcttctccc tttttaacta caatggtttg aagcttataa aaatcattat
    12781 actaaggatt ttttaatacg atgacatgaa ggagtatgaa tattttttaa atctggctga
    12841 aagcgctatt tccaagattg accttcccga gttgccttca aggctttatg atccgatccg
    12901 ctatatactc gggatgaaag gcaagcgaat acggcctgtg ttgactttgg tctcatgcga
    12961 cctgttcggt ggccatgcgg acgatgctat tgacgctgcc attggcttag aaatctttca
    13021 taactttacc ctgctgcacg acgatattat ggatgaggct atgctgcgaa gaggcaagga
    13081 tactgtaaat aaaaaatgga acataaatat tgccatcctt tcaggcgaca cgatgtttgc
    13141 gttagcatgt cgttacatgg ccttgagatg ttcggtgcga cgtgaagaga tttttgatgt
    13201 atttactaaa acggcgatag aggtgtgcga agggcaacag ttggatatgg attttgaaac
    13261 ggaaacggaa attaccattg accgttattt tgaaatgata tcgttgaaaa cagcagttct
    13321 gttaggcgct tgtacaaaaa ttggcgcctt atgcgcaaat gctgcttccg agcaagccga
    13381 catgctatat gactttggga aaaatgctgg aatggcattt caggtgcagg acgacctgct
    13441 tgatgctttt gggaataccg acaagtttgg taaaaatgta ggaggcgaca tcgttacaaa
    13501 caaaaagaca ttcctctacc tcaaatgcct tgaagtagct aatgatcatg ataaagaaag
    13561 attgaagagc atgttttccg gcacagaaac tattgatcct gaggtgaaaa tatcacaggt
    13621 tattgatatt tacaataaat ataatatcaa gcaattggca atgaagcagg tggagtcttt
    13681 tttcaatcag gctacggata ttatgcaaat ggtaaatggt gatccttcaa aaaaaatgtt
    13741 gtttctcaat tacgttgaat ggctatataa gcgggatttc taaaaggaat catctttgtt
    13801 aggcatcact tgaaaacgat ctgcatattt tcttcatcaa tctcatattc tcctaccatc
    13861 agtaaggtta tttcgccgag cataagctga cttgttagct tatggtcaac ctgaaattca
    13921 atcttttcaa gtttctcatt accaatcata aatgatttca cagtaaacag cgatctgttg
    13981 gcaatagatc catccttgaa aatattgtcg ggattgcctt gaaaatcagc gcgtgtgata
    14041 gctccgtctt tcagtaggcg catagcgcta ccaagcgaaa tggtaggtcg gagtgttttc
    14101 cgatcgtaga tgaaatttaa ttttattcca ttaacaatgc aagttgcttc gatggctccg
    14161 gttggagttt tggtgaatgg tataatattt ttttcaggct cggtgattat cttaatatca
    14221 acgctgtcgt gttcgatacg tatttttggt ttagggataa aatcgtttcg gagtattgag
    14281 aattcggtac gccggtttag ttgatgtgca gcttctctca ttggtagtgt aggcaaagag
    14341 tctatatacg attcggtaag tatagttcct gctgtgaagt taaatccgtc gcgtgtgata
    14401 tcagtcttaa gttttcgcgg agcgcgttcg ccataacctt ttgcaatcaa acgttcgggg
    14461 tcaatacctc tttgtatcag gtaattaacg accgattcgg cgcggcgttg cgaaaggata
    14521 tcattggatt ccaatgagcc gcgggcatcg gtatgcgacg caagttcgat aacgagagtt
    14581 tcattagcat caagggtacg aataagccct tgaagcgaat cctgaaactg cggctttaaa
    14641 tcccattttg ccaagtcata aaggatttca ggtaagagga tcggctcagc cggtataggc
    14701 tccaactcga agttgatctc gaaatcttta cttgcatcta cgccaagggt ggtctccatg
    14761 gctttttcga taaagtattt tggcttttcg gtaattagtt caaaggttat gcctggcttc
    14821 acttgcccgt caataaactc gtaataacca acagagttgg acttcacttg tactgttgtg
    14881 ccatcagagc ttacaaggct gatcagagcc tcttcaattg gctctaatgt atttttatcc
    14941 aatactttac ctctgatatt atagatgatt ggaggactaa taaaatagta aatgtcgtcg
    15001 ccgccccgtc ctccttgtct gttggatgag aaaaagcctt cgttattgcc ttcggggtgc
    15061 catgttatcc caaagtcatc agcatttgtg ttgataggtg gcttcatatt aacaggctcg
    15121 ccccacgtgc cgttttcttg ttttactgat ttaaagatat caagtcctcc cattcctggg
    15181 tgtccatccg aagcaaagta tagtacgcta tcgcctctaa gagttgggaa cagctcgtcg
    15241 ccgggtgtgt taatcagctc gccgaggtta cgtagcgctg tgaagttttc gcttgcgcta
    15301 ttgcgtgtag ctatccaaag gtcgcgacca ccggcgccac ctttccgaga ggaggaaaag
    15361 ataagggtgg tttcgttggg actaatggca ggatgtccaa agactgaagt gctgtcgcca
    15421 cctaaatcaa ccattatcgg ttcgctccag cttcgccctt gcttgctggt tacccatatc
    15481 tgacagccat tttccgtaag attatcgcgc gagcatcttg tgaagtacat acggctgaaa
    15541 ttctcgttaa atgcggcttg cccttcgtta tcttcggtgt ttaatgcttc tgtatcgtca
    15601 atggtaaccg gctgactcca gtttccttta gcatcttgtt ttgtagtgaa aaggtcagtg
    15661 aagttcaggc cagtccattc atccggtttt tttcctacta cgccatccct tgaggtggtg
    15721 aagatgataa ccctgcctgt cggatcgcta tagctgggcg aaaaatcatt ctcacgagag
    15781 ttaatctttt ttattgtttc aattttgaaa ttggacggat tcttctccca ttcaatcgcc
    15841 aacttacacg attgcatacc tactaatcct aacggatcac cggcgactct ttcaaggaat
    15901 gtttcatagt tagaaagcgc ctcttcgtaa ttcccattgg cacgttgcat atctgctaat
    15961 cggagtaaaa caagcggttc gatacgatcg taaccagttc gggtaagacg tttgtaaaaa
    16021 gcagcagccc ttttcggttg gttcgtaagg cggtaacact cacccatctg ataagtaata
    16081 cgattgcgtt ctagcttgtt tcttttcgat cgtgcgtaag cctttttata tttgggtatg
    16141 gcaacagaaa agcgcagatt gttaaaatct tcatccgctt gttgggactt ttttgtttgc
    16201 gcataaacaa agtcgaacgg tgttaaaaat attaggagta gtaatgatat taatagggag
    16261 aatggcttca ttgtatgttt tggaatgctc aactatttat cttgtttatt tttagtttta
    16321 ttgttacgga ttatttggca cgttaacgga ggtttaatcg gctttattat tcaacaggca
    16381 tgctatctgg taatgatgtt ttttgatcac tctctttttt ttgttcggca tcgggttctg
    16441 cttcattttt attagcctcc ttgttttgca atttgttgat gtcctctcta tagtcagaat
    16501 cataggtttt tttgttgtat tctgtatcaa gggagcttat ttgctttaat ccttcggtga
    16561 tgtcattttt aatattggtg gcaacattgg ttatatcgcc ggtttctttc tctatttcat
    16621 ctcttatctg gtttgttgct tttttgattt catttatccc tttaccaaaa aaccgagcga
    16681 tctccggtat tttttctggc ccaaagatca gaaagattac caaaactatg aggaataatt
    16741 ctcctccacc gatattgaaa aataatagag tcaaatactg tttttattaa agagttttcg
    16801 gtggcaaaaa taaaaaaaag ccccatgctg aataatattg gctggggctt ttacttttta
    16861 caagttttat taagctttgg tttttacttt aactttcctt ctgctcaaca aatcgtacgc
    16921 aagcgtactt gccagaaaga tcgaagaata ggtacctata aggataccaa caaaaagcgc
    16981 aaaagcaaat ccgcgaatta cctcgccgcc aaagatgaat atcatgagca ataccaccag
    17041 agtagtacct cctgtgttta ttgttctgga taaagtcgag ttaataccat cattgatatt
    17101 agtcatcaag gttcgtttcg gatagaggga gaaatgctcc ctgatcctgt cgaagataat
    17161 cactgtgtcg ttgattgagt atccgatgat ggtgagtatt gccgctataa atgcctgatc
    17221 aatttcaaga ttgaagggca atacattata aaataatgag aaaaggctga tagttatcat
    17281 tgcgttgtga aacagcgcta ttactcccga aagtccaaac tgccatcttt tgaaacggat
    17341 tgcgatgtaa ataaacatta tgattaacgc aaatactacc gatagcactg cgcggtttcg
    17401 gatatcggaa gcgatggttg gccctacctt ttgcgaactt aatatccctg tgagcttctc
    17461 ggaatcagta acggttacaa actcatcata ggatattgac ttatcgttat agaactgatt
    17521 aagactttcg aaaagcttac gttgaattat tgagtccatt gcaggcgagt tatcattgat
    17581 gttgtatttg gttgtgatct ttacctgtga tgtggggcca aaagtcttaa cttcgggctg
    17641 ttgttcgaag ggttcaacca aagcttctct gatttcatta gtgtttacag gttgatcaaa
    17701 acgcactacg taggttcgtc cacctgaaaa gtcaactccg taatttaatc cgcgtgttgc
    17761 caatgagaca agtccaataa aaataatggc tgctgagagg atatatgctt tttttctcat
    17821 gccaaggaaa ttgatgttta ccgtagataa tacattaatg gtcagtgagt tgccaaaagc
    17881 tattgacttg ttgcgagaga gtaaataatc aaatactaat cttgaaataa agattgcgga
    17941 gaatagtgat gaaacaatac cgatgataag ggttgtagca aatccttgaa ctggccctga
    18001 accaaatata taaagtacaa taccggtaag caaagtagta acgttaccgt caataatagc
    18061 tgaataggaa tttttatatc cgtcacttag ggcgagtttg gctcctttac ctgctctaat
    18121 ctcttctttg atacgctcaa aaataattac gttggcgtca acggccatcc ctaaggttag
    18181 tacgatacct gctattccgg ataatgttag tacagctcct aatgatgcta atacaccgaa
    18241 gatgaataag atatttgtga gcaatgcaag gttggctacc aagccggctt tgttataata
    18301 caaagccata tacgccagca ccatgataaa tgccagtacg aatgatatta atccatttgt
    18361 tactgctata cgcccaagag aggggcctac aatggcttct tcaacgatat gtgcttgtgc
    18421 gggaagtttt cctgctttaa ggatattagc gaggtcctga gcttcagcca cctcgaaatt
    18481 gccagttatg gatgatctac cgcttgggat ctcatcattt acattagggg cagaatatac
    18541 atagtcgtca agtacaatag cgatctggtt acctatgttg tcgccggtaa gttgtttcca
    18601 gatgcgggcg ccctccgagt tcattaacat tgttacctca actcttccgc tggttggctc
    18661 gaaatcctga cgggcatcca caataacccc tccgtcaaga gcaggagtgc catcccttga
    18721 tgttactttt agagctacaa gttcgagtag gtcgggagcc catttttctg gtttaacttt
    18781 ccatgcaagt tttaggttac gtggaaaagc gtcaccatct ttgttgattt ttcgcagata
    18841 attgttgata cgtgccgtat ctttaatttg ggcaaaacca actgttgcat taggtgcagg
    18901 agcccatcca ccgccctgtg tttgttgtag gttcgggcga aggtatgcaa atagagggtt
    18961 ggtctttgca agttcctctg acgacatggt ttgtgtatcg ctggtagcat caccaatttc
    19021 atccaacaat ttcgattgag ttgtatcagc ttcttcagtt atctctacat cttcaatagt
    19081 ttcttcagca gcctcagttg tttcggctaa ttcttcgtcg gtaataatct cttcgttact
    19141 atcggcttct tcttttgatt ctgcttcact ttcaagcttc agtatatcaa caagtttgct
    19201 gtcggcagct tcgaaataat tatagaggtc ggggaacttg taggtttccc aaaactcaag
    19261 ctgtgcagta ccttgcagca gtttcctaac tcggtctggt tctttaatac cgggtaattc
    19321 aacaaggatt cgaccggcag tttgtaattt ttgaatattt ggctgggtaa cgccaaaacg
    19381 gtcaatacgt gagtgaagta tattaaatgt gcggtcaatg gcttggttag tttcggttga
    19441 cagaattcgt agaacatctt cattagttga attgaagttg attttatctt taaactcagc
    19501 cataaagagt gatgctaact ggaaattagg atcaatttcg tttattgcct gcccgaaaag
    19561 ggcaacgaaa tcttcctgac tatttcgctc ttttaatttg gcgcgctcga tagcttccac
    19621 gaaaaccgga ttctggctgt gccccgataa ggcgactata atgtcggata ccgccacctg
    19681 caaggtaaca ttcatacctc ctttaaggtc aagccctaag tttagctcgc gctcttttac
    19741 ctctttatag gtgtattttc taataaggat attgtaaaca atctgatttg acatcgaatc
    19801 cagataataa acttcttttg atctggaaat agagtcataa agaaattgat acttagttaa
    19861 gtcgtctcct gcaagagcgt ttgcctgcat ttccgtttcc tggccatatg caaaagccct
    19921 agcttttttc tcaacccttg cggtaacaaa tgtgaaggat agatgaaaca aacaagctaa
    19981 agcaaataaa atagcaatgc ccttaatgaa tcctttgttc tgcatgtgtt aattgatttt
    20041 atagtaaatt acttctgttt ttgactactt taaaaattaa gggtgcaaat atagaacaac
    20101 atttattatt accaataacc tgtaatcaac attttctgta gagaatactc tatacctcat
    20161 ggaaacagca agttttaaac caaattacaa atggtaactt tataaaaaag ccactataaa
    20221 aagctgtagt ttttagcata aaaaagcatc tgatcgctaa aattttcagg ataatgcata
    20281 gaaatgtcta aaattttgcc gtttttttta ataggttgta atacgggatt tatgaatcct
    20341 gaaaatttag ggattcctaa tgatttccaa cgttgtaggg cttcttgatg cagttttctg
    20401 tcaactttta caccatattt ttcaattagg tatttcgccg ctctgaaatc tccttccgat
    20461 tttatgcgtt gtacttcatg tagcagctta ccaaagattt ctcttagttc gtcgtggtta
    20521 ttaataacaa aaaaagtttt tccatcacgt ctcttttttt caatgatatt ttttttgaat
    20581 ccttgttcat acgcccattt agcaataagt tgccggtttc gcatgtgcga ttcttccaaa
    20641 tttttaccca tttcaactct ggcaagttgt gtgattaatc caccggttat gtaagagttg
    20701 tattcggcat aaccgacgtc aatcgagggc gctaatccaa gctcaacaag ctttggatca
    20761 atggcaaagt agagtgcaaa gaggtcggcg cgagcctctt cgattgtgga agcataattt
    20821 tttagagttt cgtgtggttg agctacgccc tctttaagcc ttcctgagcc atggccaatt
    20881 atctcgtgaa gatcaacatg cagttgtgta gctaacttat tccatttcct ggataactca
    20941 atctcatatt ctgaccaata gaactcctcg attatacctg attctttatt agcctcggtg
    21001 tgggcatcaa tgatgttggc cagtgtaacc gatttcgaac catgctcagc tcttatccag
    21061 tcagcatttg gtaaatttat gccaataggc attgaagggg agcaatcgcc cgatgctaca
    21121 actacattaa tacttctggc agatactcca cttatactct tttttttgta ttcctccaaa
    21181 ataggggagt gcatctcaaa ccactcggca tttgtgctta tgatttctgc acgatgggtt
    21241 ccctcaaaat catgtaactg cacaaccgat tcataggtgg cttttctgcc taacgcatcg
    21301 ccataaacct ctataaatcc gttgataaca tcaacatcgc tctctgtatc attaagccat
    21361 aaaatattgt actcatcaaa tgtctggaga tttcctgttt tataaaattc aactaacttt
    21421 tcaagtgcgg ctttttgacc gtttgtttca gctacttcga tactcttttc taaccaaaag
    21481 acaattctct caattgctgc cgagtacata ccaccgactc tccaaactct ctcttccacc
    21541 ttgccatcca cctttacaag ctttgaattt aatccaaacg acaatggtct cagagggtct
    21601 ggattttcca ttgaagcata aaactcctca gcttcttttt ggtttacgtt ttcataaaaa
    21661 ttattggcag aggcggctaa cagatcaaca cctttgtcga gaacaacgcg tttggctgca
    21721 atttcaggat caaagagcaa aggagtaatc caatggataa aatcctctat gttttcatta
    21781 tccttcagaa aatcaggctt tggggtatct gttaataggg aagtgaaata attttcagaa
    21841 aaatgaggga caattttttc catcgaataa tggtgatgaa ttccattgga aaaccaaatt
    21901 tttttcaaat atgccaagaa caggttccag ttttcatcct ccttaagttt ctgactatat
    21961 tttaggatat gttccaatgt ctttctgatg agcaagttat agcgatagtt ttgatcccaa
    22021 ataatatcgc gtcctgataa tgcagcttcg taaagataat agagcaatat ttttttcttt
    22081 aatggcagat tttcaaaacc tgtgacttga taacgcaata ttcggtagtc ttcaaattcc
    22141 tcagcgatta ctttaaagtc gtttttgcta ttcattaaaa tttgtgtttt agctgatgac
    22201 ttagcctatt tgttttttcg aattttctta aatgctgcgc caagattttc ggtgatgtct
    22261 ccagctatca ttgcttcctg cgatttatct tttgaggcga tatcacctgc aagcccatgc
    22321 agataaacac ccagtatagc agcttcttga ggatgatatc cttgcgccag aagccctaat
    22381 atgactcctg tgagcacgtc gccgctacct cccgtagcca taccagggtt gcctgtagtg
    22441 ttgaaaaaac acttgccgtc aggagttgag atagaggtaa aggcgccttt taatacaata
    22501 tagatacgat gcataaccga aaactctatc tgacgcttat tacgttcgaa agcattggcg
    22561 aattttccga aaagcctttc aaactctttc ggatgcggag tgaaaatact aagtggaggg
    22621 ataaacgata tccatgtttt attttcggct aagatgttaa gtgcgtcagc atcaaagagt
    22681 aaaggtacag tggcgttttg aatgagtaat tttaaagctt gttgtgtctc tttagctaac
    22741 ccaagccctg gccctatagc aatggtatta aataacgaca aatcgggatg atggctgata
    22801 aaacgatcat tattatcaat acttaccatt acttcaggag cggctgtttg cattatctgc
    22861 cggcttgagg caggtaaatg ggcagtaacc aagccggcgc ccgaacgtaa ggcagcttga
    22921 gcggccaaca cggcagctcc cgtttttcca taactgccgg caataagtaa tgcatgacca
    22981 aaatgacctt tatgcgaaaa ttgtgttcgg cttttcaaca gtgagaaagc atcagccctg
    23041 tcaatagtga aatttctgat ctccttatct ttagttgctt catccattaa tccgatagga
    23101 agaacaatcc attttcctac ataatgctca ttctcagcaa aaagaaatga cagcttagga
    23161 tattggaaag taagtgtgta atctgccttg acgattgccg ctttgggtag agcagaatgc
    23221 tgatcagcat acaaccccga cggtatgtca attgctatgc gggtagaatt ggtatcgttg
    23281 ataagcctaa ttacttcggc tgcaaaacct tcgattggtt tggatagtcc agagccaaaa
    23341 atggcatcta ttataatact accatcaggt atgtcaggaa gatcctgaag cgtagtaacg
    23401 ttgtaaataa ctgctttttt actcttttcc aaacgttcga gatttgtgat aaaatctgga
    23461 gaaaatttat tgctgatacg aagaatgatc gtaacaacgt ggtatccgtc agcgataagt
    23521 aatctggaaa tcaccagtcc atctccgccg ttgttacccg gtccacaaaa gatattgatc
    23581 ggagcatctc ttctgacaac tttttttatc catttaaaac attgataagc agccctttcc
    23641 attaagtcaa tggaggcaat aggttcgttt ttgatagtat aggcatcagc ctcgcgaatc
    23701 agctctacag gaagaatctt catgacaatt ttttttgtaa aaataatgcc tttgtaaaaa
    23761 atgtgctttt agttaagaac agtgcaaaac agcttaatac gtgaaaaaga aaaatctaaa
    23821 aaaggataaa tagttttctc tttttgattt taaacggaaa aacttgaaaa aacttttttg
    23881 tttacttttg aagctgattt taaaaattat accgatcgaa tatctcaaat tgatgaactg
    23941 ttaataaaac gactatcaac actggcatta gtatgcgaat caatagtatt actaagaaat
    24001 tttctaaaag agtcattgca actttcgggt aaaaaacata aaaaatctga tgattaatta
    24061 tctccgcaat tttattgttc atttaaagat tcaaagggaa cagagaaacc tttttcgtac
    24121 caaaaaggtt gtcaatttga ctgaggcatc cactattgga attttgtttt tgatggagag
    24181 cgaaga
//
LOCUS       DAOOAI010000022        24146 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_2731_length_24146_cov_5.743473, whole genome shotgun sequence.
ACCESSION   DAOOAI010000022 DAOOAI010000000
VERSION     DAOOAI010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 24146)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24146)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24146
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2731_length_24146_cov_5.743473"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            119..610
                     /locus_tag="PK345_02835"
     CDS             119..610
                     /locus_tag="PK345_02835"
                     /inference="COORDINATES: protein motif:HMM:NF022968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3225 domain-containing protein"
                     /protein_id="HPX33989.1"
                     /translation="MKKFTLLSSLLFVFLLTACNTIKISDKEKEKALVKNVLDKYRTA
                     NENQDINLIEEIWSPDETIISFGTERGERLVGFSQIKEVVQRQFDTFSETFITPANQV
                     IEISDDGKTAWFSQMMNYNFILNGEPYSFRNLRYTGVLVKNEGKWKLVQTHMSVAYNP
                     LEK"
     gene            complement(621..1661)
                     /gene="pheS"
                     /locus_tag="PK345_02840"
     CDS             complement(621..1661)
                     /gene="pheS"
                     /locus_tag="PK345_02840"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019151894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HPX33990.1"
                     /translation="MITEIEKILAEINTFTAGSDDELEAFRIKYLSRKGKIPSLFERF
                     KDVLPQDRKVIGQSLNNLKTQAQEKYNLLKSEISEKTITKKGEYTDLTKPTGFQDIGA
                     RHPISVVRRMILEIFARIGYNVAEGPEIVDDWHNFSALNFPEDHPARDMQDTFFVSNP
                     LTDTDKPILLRTHTSTVQVKMMESAKPPIRIVAPGRVFRNEAISARAHCIFHQVEGLY
                     IDHNVSFADLKQTLYYFATEFFGDDTQVRFRPSYFPFTEPSAEMDILCKMCNGEGCRI
                     CKYSGWVEILGCGMVDPNVLELCGIDSKEYTGLAFGMGIERIAMLKYQIDDLRLFFEN
                     DLRFLKLFEGAV"
     gene            complement(1681..2241)
                     /locus_tag="PK345_02845"
     CDS             complement(1681..2241)
                     /locus_tag="PK345_02845"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008860918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPX33991.1"
                     /translation="MVKFIIITEYGDIKGVLYNDTPQHRDNFIKLVEEGWYNGSIFHR
                     VIKNFMIQGGQGATGKQDPGYTVPAEFVPAHYHQKGALAAARMGDNVNPQRASSGSQF
                     YIVQGQTFNDQMLDAMEQRSGIKYTEEQRRVYTTVGGTPHLDGAYTVFGLVTDGLDVV
                     DKIAAVQTGSADKPVNDVEMTIKVIK"
     gene            complement(2325..2915)
                     /locus_tag="PK345_02850"
     CDS             complement(2325..2915)
                     /locus_tag="PK345_02850"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006848476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPX33992.1"
                     /translation="MQKVIISTSLGSMTAILYNETPIHRDNFIKLAKEGYYDGTLFHR
                     VIKNFMVQGGDPDSRNAKPDQHLGVGGPDYTLQAEFRPEFIHKKGALAAARKGDQTNP
                     EKRSSGSQFYIVQGEKVNQAELSNFAARGGVTYSPDQIKIYESIGGTPFLDQQYTVFG
                     EVIDGFEVIDKIANVQTGANNRPVKDLKMTVKLIDE"
     gene            complement(3206..3796)
                     /locus_tag="PK345_02855"
     CDS             complement(3206..3796)
                     /locus_tag="PK345_02855"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018696311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HPX33993.1"
                     /translation="MFLEIKSESGSRRGWIEVICGSMFSGKTEELIRRLKRARISKQK
                     VEIFKPAVDVRYDSENVVSHDLNLIASTPVEASTQILLLATDVDVVGIDEAQFFDSEL
                     PSVCNQLANRGVRVIVAGLDMDYTGKPFGPIPALLATAEYVTKVHAICIKCGNLAQYS
                     HRTAGGTKLVELGEKENYEPLCRSCYQSSVENRTKK"
     gene            3922..4731
                     /locus_tag="PK345_02860"
     CDS             3922..4731
                     /locus_tag="PK345_02860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33994.1"
                     /translation="MEQKSTSVQEISQILNKIQKHWEKVLENKESFSKIDKDILMNDL
                     RKVYELISDINKIESSNEFAVKSPFSSSKILFEDEPVATIHHNEKSKLSNSGYYEDDS
                     IDRDNKTENRNLMLDFEKERTEKTSFPTQTSGEKGTPTSEQNPVSYTQGTVRVANQQK
                     TMIDLFTPQKTVSDILQDNVANTVAAKIQNNHIDNIKTVIGINDKFTFINDIFKGEIS
                     KYNEAIDRLNSFKDFQEALNYIKSSGFETGTTENQIALEKLIKLLKRKFYE"
     gene            4764..5555
                     /locus_tag="PK345_02865"
     CDS             4764..5555
                     /locus_tag="PK345_02865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX33995.1"
                     /translation="MKKNLLPLFFILIVFSFSSCTKIKREYYPLTSINLQMIENITPE
                     DRSIYFHFYTIELYSCLNYLIRYDFSSDNGNLTINLRDVEKNDLCINGQGPATASMNL
                     GKYDNGTYQINLTVAGETNTGALEVSDLGYVLHFQTLNKLNLIADTMFRIPENIFWGY
                     VAYPKEASAGIANQVLDSLESHGASPHNLQHGYYGYFTIKENGEITLLDKSDANFSIN
                     FIYKQEDPDDNIKDVIRYFNNHHLNEVYIFVRTSKGLVFNSPMNI"
     gene            5674..9078
                     /locus_tag="PK345_02870"
     CDS             5674..9078
                     /locus_tag="PK345_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPX33996.1"
                     /translation="MKNGFTLFLLTVLFATGINLNIFSANPQLISNRLTEQIETSRDG
                     ELIKINIIMREKFDTYNLLRNVEDMEKEQRRSYVISVLKEFASLSQRGILEDLNMLQL
                     TDQVSDVNALWIINIINCYATPTAIYKLAERADIELIDHDFYQIILDPSENKEAYFEE
                     GNVNNREITWNVNKVNAPQVWALGYDGEGVIVALIDTGVNYNHNDLKDHVWVHPDFPY
                     HGYNFANNNNNPMDDNGHGTHCAGTVAGDGSSGSQTGVAPAATIMCLKVLDSSGGGQQ
                     SSIWSATEFAVEHGAHVLSLSLGWLHAWGPNRQLFRQAFDNVLAAGVVSSVAAGNEGE
                     QQGLYPVPDNIRTPGDCPPPWLHPDQTLTGELSSVICVGATNSGDGVAGFSGRGPCTW
                     QSVSPYNDYPYPSDIGLIRPDISAPGVNIKSLAHYSNTGYESGWEGTSMATPCAAGIM
                     ALMLHKNPLLTPAQISQILEETTVVLQPGKNNNSGSGRVNALAAIEATPGPGPAYYSH
                     SINDETGNNNGQLDPGESVKLTVAMANFSEMVVDSVTVNISTQSPYVTITDGTEEYGN
                     FDLNSIIEIEDAFAFDVANDVPGGEAIKFFLLAENDYDSWESSFTITANGVTITSSNF
                     SINDFSGNGNGNLDPGENADILIHINNLGQITAPNVICTLTSTSSDITINNGSFTIPV
                     IEPGQSSQANFSVSVSPTAQIGSIAEFNLEVVTGYYQYDFSFFPKIGLIVEDFETGDF
                     TQFPWMFAGNQPWTVTNTGAYQGVYCAKSGTINNNASSEMKITIEASGGDSIVFYRKV
                     SSEANYDHLRFYIDNTKVGEWSGETAWGRVAFPVTAGTKTFRWVYIKDIFFSSGQDCA
                     WVDYIEFPSVVDNSLAVNAGADATICEGTSHQLDATAQNYTSLLWSTSGTGTFSDSTI
                     LNPIYTPSEADINAGAITLSLTVYNEGGESLTDNLILNFLLLPSTPEAITGLSQVCMG
                     ENETYSINQVANAEYYNWILTPANAGSFAGNGTEIVISFSNNYTGAASLKVCGINNCG
                     IGNFSEEFNILVEDCTGINELDTSGKFFITPNPNDGSFMIHLNDNISRTNILKLMNTK
                     GQVILQLQLEDSDQIQMNTKSLENGMYFIMIENEQSVSIQKLIINK"
     gene            9193..13113
                     /locus_tag="PK345_02875"
     CDS             9193..13113
                     /locus_tag="PK345_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPX33997.1"
                     /translation="MKKVLLTILTLIFAVSLNAETVKKNYHFSNPTFETIKGYQQISF
                     DGLLLTGQAGEPTLPYQSVVLLIPPGHTALNIKLDFGEEIEMEGVYNIYPMQYVQPVS
                     KGKSGIFAFKEDVYKKDAFYPEKMEGVISTHFMNGFAVAISSFTPVRYNPYKGKLSYF
                     EDVTVTIETAPDSKAIKALENINTSPAVLQRLALLVENSEAAVNYPKREVRNDDYDLL
                     IITAAQFENAFQPLISFYQPRGIRTKVVSTQTIYSTTSGQDNQEKIRNYVIDQYQNNG
                     IQHLTLAGDVEHVPYRGFYCSVQSSSVYTDNDIPSDLYFSALDGTWNNNNNNLWGEIG
                     EDDLLPEIGVGRMSFSSQSELSAMLNKIYKYVGEPVLGELRDPLLAGEHLYNNPLTWG
                     CDYLELLIGYHDDNGYITNGIPTDHNIMTMCDENGTWSANQLKQKINQGTSFIHHCGH
                     ANSNYVMRFYNSDITNSNFSQLNGEIHNFTLAYSHGCICGAFDDNDCIAERMINIDNL
                     AVAVVMNSRYGWFNEGQTEGPSAHLHREFVDALYTTKEPHIGMTHTLSRIKTAPWVNA
                     PGQWEEGALRWCFYDCNVLGESALRIWTDEPVDITASYQNALPIGVPSIQISISGNGS
                     VEGLACTLIKEGVTHGTAITNSSGLAEIIFDPPITELGEAIIYVSGYNCLLNEFPLIV
                     IPNDGAYVIYESSLVNDSQGNGNGLPDYTESILLTTTLKNVGSSQADNVTASLTSANP
                     YVTITNNSAYFGTIPGQGSLTIEDAFEFDIAGNVPDQQAILFNIEAVGQNNWSSGFSI
                     IANAPNLVKDDIIINDASNGNNNGILDPGETADMVITIINSGHAEAYSVKASLTSADQ
                     YITVNTTTPQSLGNMTPEQSIPATFSVFASPDTPAGYTGQITLNITAQHGVSTTQTIN
                     LNFTDYCYPTANCSFGDGITGFGLASINNMNNGCSNNGYGNFTDLSTELEPGQTYQVS
                     FKTGYSNQKVCLWIDFNSNKEFEQSERLVTDFSLSNSNQVYTTTITIPESITGGIKRL
                     RIRARWMDSAQDPCANFTYGETEDYTVIFPAGQMSINAWCTPDEICLYDSTQLIVTAT
                     GGTGNYTYLWTPAAGLSDPTIQNPTASPSETTTYTVEVNDGSSSINTDVLVIVNSLPP
                     TPTIALTGNTLTSSSPTGNQWYCSQGAIEGATGQSYTCTWEDVYFVKVTNENGCVSMQ
                     SNSIHVVIYSIDDFEHETDFNIYPNPFNDEVFINSKNSSDKVLYVSVFNNLGQEVLKT
                     KINSKSTSSDEIIKISTAELDNGMYFFKIYSENRVIIIKLIRNR"
     gene            13215..14021
                     /locus_tag="PK345_02880"
     CDS             13215..14021
                     /locus_tag="PK345_02880"
                     /inference="COORDINATES: protein motif:HMM:NF019737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family peptidase propeptide
                     domain-containing protein"
                     /protein_id="HPX33998.1"
                     /translation="MKKLIFSILAILLSFISFSQKSWVPLTSEQPLQSNIAILSSDRS
                     GLLLEVTIPGMFSEVITHEEMTFQRITLDDNRTTKEVGRPELPMLHKLIGIPGNQKIR
                     VSVLSMETKKLQGYFIYPFQTPTTDNPGGHNKPFVMDKSFYQKNAVYPLENFIVSQPG
                     IWRDVKVAGVEVTPFNYNPVTKELQIVTRATLKVEFYGTDERTTFNPKMELTPKFYQM
                     YSAAIENFDDLGYTLTYRDNSNTKYLIITNNEAVSTIQPLIDWKNQMGIK"
     gene            13994..17623
                     /locus_tag="PK345_02885"
     CDS             13994..17623
                     /locus_tag="PK345_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPX33999.1"
                     /translation="MEKSDGHKVELKLIEPGFNTPQHFKTYITELYNNQGLEYILMVG
                     DAYPNGGNSGGPNVVPMFYWAPGGGEDPSYSDSWYTCMDGPDDHYADLAIGRFVYNIN
                     QLAQLQLQIQKTLNHYMNPDLSSNWAENTILIAHKENYPGKYTQCCEEIRTFPYALQT
                     PIFEKAYGGQGYSNAQVVNYVNTTGCGIFNYRGHGSTTELWQWTTVSPMSFTATQVAQ
                     LTNPSRLFVFFDVCCDNMDIVSYAGECLCESFMKHSGASVAVNGAIIPSYTIPNHDYD
                     KEMYKAVYHEGITNIGYVTNFANLTVLNVHGNIGRSNVRTYLWLGDSSLEPWTKLASE
                     MTVTHDDQLFLGLSEYPVQVLANGIAVENAMVCVSNEEGSVYAVGYTDGTGFVNVQFD
                     SPVEFPGEATVTVTKFNCLPYQATIPIIPQDGPYVVKDSWLINDNVGGNGNGLMDYAE
                     SILLTLSVKNVGTELAQNVNVTLSTTDPYIIITNATASYGNIGPNASVSLTDAFAFDV
                     AENIPDNHSVLFAVTATDGTNIWNSNIVITGRAPVLAYDSFTILDPNGNNNGKIDPGE
                     EATIVVTVKNTGSADATGVLGLLSTNDAYLQVTQIGAVAIGNLTSGDSGTAFFSVYAS
                     QNAPAGHSAELSINITADHNLSETDNFNVIIGQIPVVIICLDPNHNSGPKIQTALNTL
                     GITSEYVTSIPTDLNLFSSAFVCLGVYSSNHVLTTPQGQQLADFLNAGGSLYMEGGDT
                     WYYNPKTPVHTMFKINGVGDGSGDLSTINGQTGTFTEGMSFTYNGDNNWIDRIEPIAP
                     AVKIFQNQSPSYGTAVAYDAGTYKTIGASHEFGGLTDGASTKVALMEEYMNFFGLIPS
                     GVTANFVADQTEICVNNQVQFTSNSIGSINEWFWEFQGGTPATSTEENPLITYYFNGT
                     FNVSLTVTGENGSNTMTKNNYIKVSPPPSAAATPTGVTALCQNAANTTYTTTGATHAT
                     QYTWTLTPTNAGVISGNGLTAVVDWDDNFYGSAEIMVSGSNNCGQGANSPSLIITIDP
                     LPSPAGEISGDTIGCQGETVLYTVETIANSESYNWVLTPSYAGSLQINNNEVNITFSD
                     KYLGDATLKVCGVNACGEGEFSNLFDIFIENCIGINELYGEKEITLYPNPSNGTFTIE
                     FNLRDIVEITIINTLGKEVYNSGQLTLSGKTYHNIEIKLSDGVYYLKAEGPKTHLTKK
                     LMIVK"
     gene            17822..21709
                     /locus_tag="PK345_02890"
     CDS             17822..21709
                     /locus_tag="PK345_02890"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPX34000.1"
                     /translation="MVFPQDIAIDFGKAKTGVTIKEDSFDHLNITLQFEGITSFHVST
                     VKGDFNEIGIPGLYSIGRLGTPKLPAVKKLIEIPHGAEFSVEIKNYSVKEYKLSDYDI
                     IYPIMPVQPSIRKDQNSEDIPFEFDENIYQSDGFLEPQLATLEELGILRGIRLARLTI
                     APVSYNPVKGVVKVVNDVEVEITFRNTDPALHQYIKASTYSPYFDVVQNTMLNRLEHG
                     YPAHPDLTKYPVKYLIVSPRMFENDLQPFIQWKTKKGFKVITGYTDQIGTSYSAIRSW
                     VQSQYNSGTPTDPAPSFLLIVGDTPQIPSTTGSASGKMTDLYYGSVDGDYFPDMYYGR
                     FSATNSAQLVAQINKTIYYEKYQFADPTYLNNITLIAGADSYWNPKIGQPCILYGTTN
                     YFNTAHGYNAINVYLNSYSGCYDPSRIAVSLINYTAHCSETSWGDPMLSKSMVNSFVN
                     NGKYPVAIGNCCLSANFGTSECIGETWQRAANKGSVAYIGSSPSSYWFEDFYWSVGAF
                     PIQGYNNGYVPTYAETTWGAYDAPFVSDYVTTGSMVFVGNLAVTEVHIQGYPSHSSPL
                     YYWQAYNVLGDPSLVPFYTQPDPNTVTHTPFFPIGLDTYEVNALPGSYVAISKDNVLH
                     GAGLVGVNGVAQIMINPVLSAGMVDIVVTKPQHIPYITQVPAAALQGPYIVLESFTIN
                     DESGNNNGKADYDEDIWLNLTFKNIGADPSSDLTVTISGDDDYTTITSLLSQSFQSIP
                     NGEMFTLNNAFSIKISSYLPDQHLSNFQLQITDGNGDWNTSFKITLNAPQLEIVDLTI
                     DDQTNGNGNGHLDPGETINLNFTLSNNGGADAKNVNALLTFDPSIFNIGSEIIMPGNI
                     GSGEQKEWFVIINVLQNAPTGIFTHLHLMLTTGQYSELKTFDLTLGVDNNIEDFESGD
                     FSMFPWQFSGNANWTITNVSPYEGNFCAKSGAISHNKTSVMQVTVINLLPGEMSFWHK
                     VSSETNYDFLRFYIDGVQKDTWSGNTSWQQATYPITAGQHTYKWAYTKDISVNSGSDC
                     AWVDLITFPINATAGALEVNIQANQTIIEAGDFIQFYDISSGFPLNFNWNFEGGIPAT
                     STNKNPMITYEQPGNYNVSLTISNANGQASKTFENYIVVTSSIISQTLYIPAGWSGIS
                     SNLIPQNTIVGNILQPILNDLIVLQNMNGVFYPEMGINTIGLWDYKQGYKIKTNAETQ
                     LNITGWQNEQQTLDLIAGWNLIPIMSNLNQNIELLFSGLPQNGLLVKEVAGTSLYWPE
                     MNINTLLELEPGKAYFVKIVNPATIYFMNDPLK"
     gene            21778..>24146
                     /locus_tag="PK345_02895"
     CDS             21778..>24146
                     /locus_tag="PK345_02895"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPX34001.1"
                     /translation="MKKISLTLLLIIYFLVTAKAEDFNTTFTYLSETSSRVEFKLDNF
                     YLQQINLGGTDYTRIDFAGRVTTMDEGFAELPFVNVNISLSPLKNLSYKIVSTKYTDY
                     LLDFPLVPSRGVIYRNQDPSTIPYRIASESLIDDYYPAQLIEMMDPYIIKDVRGTTLY
                     FYPFRYNAVQRILRVYTEIIVQLDEDDTDPVNPLLNPSGRYFTEMEELYRSVFINYES
                     PVDYPNIGETGDILVITTTRDETAIQPYINWKKEKGYNVWKEVVTAGTNVKNLIKQKY
                     NANPAILFVQLVGDWADIKCDIGGEENAPMDPMLGCVVGTDNFPDIAIGRFSANSDTH
                     VTIQVNKSINYEKNPSGVWYDKAISVASNEGAGIGDDGEIDYQHTNIIYNYKLNPFTY
                     NTHYTVYEPSGTATMVKNAIENGASIINYCGHGSKTSWGTTGFNNNNVNQLTNGNKLP
                     FIFSVACLNGAFHSGDCFAEAWLRKNNGGAVWTIMSTVNQPWQPPMRGQDYFNDILTG
                     GYNYNTNPGNGINTTEGRTILGSIALNGLVLMYTESSNSSDLKTLQTWTTFGDVALQA
                     RTMTPKTLTLTNTNLQAGLPFETIVKANGAPVKNALVALSQNGTYVKAYTTQNGAVSI
                     PNNFIPGDVLLVVTAFNCNTIYQTIQSAPAAGPAVYLDEVTINDTYGNNNGQLDYGET
                     VGLNVSLKNSGLELAKNVNATLSCNDDYITILNGNTFFGNIGIENTVTVSNAFTIKAA
                     PETPDNHEVLFSIIATSEGSSWESFFSLTAYSAVLNYDSHTISDPTGNN"
BASE COUNT         7759 a         4865 c         4457 g         7065 t
ORIGIN      
        1 attatttcaa tgattaaaat taaacattat attagcagcc attaatttta atttggcagc
       61 tctgcattaa ttaaaaagaa aaggttttca atgatatata atcaaaaaac attctattat
      121 gaaaaaattt actctactct caagtttgct ttttgtattt ctccttactg cctgcaacac
      181 tattaaaatc agtgataagg aaaaagaaaa agcattagtt aaaaatgtac ttgacaaata
      241 ccgtacggcc aacgaaaatc aggacataaa cctgattgaa gagatctggt ctcctgatga
      301 aactatcatt agtttcggaa ccgaaagagg tgaacggctt gttgggtttt cacagatcaa
      361 agaggtagtt cagcggcagt tcgacacttt ttccgaaact tttattaccc ccgccaatca
      421 ggtaatagag ataagcgatg acggcaagac ggcttggttt tcgcaaatga tgaattacaa
      481 cttcatcctc aacggagagc catatagttt cagaaaccta cgttacaccg gagtattagt
      541 aaaaaatgag ggcaaatgga aattggttca aacacacatg tcggtagctt ataatccgct
      601 tgaaaaatag ggagataatt ttataccgct ccttcaaaca acttcaagaa acggaggtcg
      661 ttttcgaaaa atagccttaa atcgtcaatc tgatatttaa gcatagctat tctctcgatg
      721 cccattccaa atgccagacc cgtatattcc ttactgtcaa tgccgcacaa ttcaagtacg
      781 ttaggatcta ccatcccgca tccaagaatt tctacccagc cactatattt acaaattcta
      841 caaccttctc cgttgcacat cttacataaa atatccattt cagccgacgg ctcggtaaaa
      901 gggaaatagg acggacgaaa acgtacttgg gtatcatcac cgaaaaattc ggttgcgaaa
      961 taatagagtg tctgtttcag gtcggcaaac gaaacattat gatcaatgta aagcccttct
     1021 acctgatgaa atatacaatg tgcgcgcgcc gagattgctt cgttgcgaaa aacacgcccc
     1081 ggagctacta tccgaatcgg cggtttggcg ctctccatca tcttcacctg aaccgtactt
     1141 gtatgagtac gcaataatat gggtttatcg gtatctgtta atggattaga tacaaaaaaa
     1201 gtatcctgca tatcccttgc cggatgatct tcgggaaaat tcaaagctga aaaattatgc
     1261 caatcgtcaa caatctctgg cccttcagca acattgtagc ctattcgtgc aaatatttca
     1321 agaatcatac ggcgaacaac cgaaatagga tgacgcgcac caatatcctg aaaacctgtt
     1381 ggctttgtaa gatcagtgta ttcgcctttt ttagtaatag ttttttctga aatttcagac
     1441 tttagaagat tatatttctc ttgagcctgg gttttaagat tattcaacga ctggcctatc
     1501 acttttcgat cttgcggcaa aacatcttta aatctttcga agagtgaggg tattttacct
     1561 tttctgctca aatacttaat cctaaaggct tcaagctcat catcactacc ggcagtaaat
     1621 gtattgattt cagctaatat tttttcaatt tctgttatca tgtaaaagta tttcaacagg
     1681 ctatttaata acttttatag tcatttccac atcatttact ggtttatctg ccgaaccggt
     1741 ttgtactgct gctattttgt caaccacatc caaaccatcg gttactaatc caaatacggt
     1801 atatgcgcca tcgaggtgag gagttcctcc gactgttgta tagactcttc gttgttcttc
     1861 ggtatattta attccagagc gctgttccat tgcatccaac atctgatcat tgaatgtctg
     1921 gccctgtacg atataaaatt gtgagcctga tgaagcgcgt tgcgggttta cgttatcgcc
     1981 catacgtgct gctgctaacg cacccttttg atgataatga gcaggaacaa actcagcagg
     2041 aacggtataa ccaggatcct gttttcctgt tgccccttga ccgccttgaa tcataaaatt
     2101 tttaataacg cgatgaaaaa ttgagccatt ataccaaccc tcttcaacaa gtttgataaa
     2161 attatcgcga tgttgtgggg tatcgttata aagaacacct ttaatgtctc cgtattcagt
     2221 aattataata aactttacca tcttttcctg tgatttcaaa ttcatgctaa aaataaaagc
     2281 caaagttaaa aataggaata ctgatttttt catcattaaa ttatttattc gtcaattagt
     2341 tttactgtca ttttcaaatc ctttaccggt cggttattag ctcctgtttg tacattggca
     2401 attttatcaa tcacctcaaa tccgtcaata acttcgccaa atacagtata ttgctgatcg
     2461 agaaaaggcg ttccgccaat actttcataa attttaattt gatctgggct ataggtaaca
     2521 cctccgcgtg cagcaaaatt actgagttcc gcctgattta ccttttctcc ctgaacgatg
     2581 taaaactgcg atcctgatga tctcttctcg ggatttgtct gatcgccctt acgagctgct
     2641 gcgagagcgc cttttttatg aataaattca gggcgaaact cagcctgaag ggtataatca
     2701 gggcctccaa cacctaagtg ttgatcaggt tttgcatttc ttgaatcggg atcgccaccc
     2761 tgaaccataa aatttttaat cacccgatga aacaatgtac catcataata tccctctttc
     2821 gcaagcttga taaagttatc acgatgaatt ggcgtttcat tataaagaat ggctgtcatc
     2881 gaacccagag atgtagatat tataactttt tgcactttta atacttccgg ctcttccgtt
     2941 tgttgtggct gttcgtcagc ctgctcaact actgcagaaa tggtttcggt ctgggattcg
     3001 gctttttgtt ctttttgtga agagctttgc tgcccacagg atgtcaagaa aaggaatgag
     3061 ataaacatca atcctaaaga gaataaagat ttcatagtaa aaacttttaa ttttaatatt
     3121 gtcttaattt cttcaaaaac tcatttcaac aatgaatatt ttataaaatg cacaaaaact
     3181 aatataaact gcttttgtgg gttggtcatt ttttagttct attttcaaca gaactttggt
     3241 agcaacttcg gcaaagcggt tcatagtttt ctttttcgcc taactctacc aacttggttc
     3301 ctccggctgt tctgtgcgaa tattgtgcaa gattgccaca ctttatgcaa atagcgtgaa
     3361 ccttggtaac atattcagcc gttgctaaca atgcggggat tgggccaaaa ggttttccgg
     3421 tataatccat atcaagacct gcaacgataa ctcgcacccc tctgttggca agctgattac
     3481 atacagaagg cagttcggaa tcaaaaaatt gagcttcatc aataccaact acatctacat
     3541 cggttgctaa taataatatt tgtgtagagg cctcgacagg agttgaagca ataaggttca
     3601 aatcgtgtga gacgacattt tcactatcat atcgaacatc tacagcaggt ttgaaaattt
     3661 caaccttttg tttcgagatc cttgcacgtt tcaagcgtcg gatcaattcc tctgttttac
     3721 ccgaaaacat tgaaccgcaa atcacttcga tccatccccg acgagaaccc gattcgcttt
     3781 taatctcaag aaacataaca ccgactatta aacattcata gaatcaaaac tcaaatactt
     3841 aaatacgctt ttaaaaattg atgcgtttaa catttggcaa atttatggaa atagatttca
     3901 taaaataatt tttctttaaa aatggaacaa aaatcaacgt ctgtccagga gatttctcaa
     3961 attttaaata aaatccaaaa acactgggag aaggttttag aaaacaaaga gtctttttca
     4021 aaaattgata aagacatctt gatgaatgac ttaagaaaag tctatgaatt aatttccgac
     4081 attaataaaa tagaatcatc aaatgaattt gcggtaaagt ctccattttc ttcatcaaaa
     4141 attctttttg aagacgaacc ggttgcaaca attcatcata acgaaaagtc taaactttca
     4201 aattccggtt attatgaaga tgattcaatt gacagggata ataaaacaga gaacagaaat
     4261 ttaatgcttg attttgaaaa agagagaact gaaaaaacat catttccaac ccaaacatcg
     4321 ggcgaaaagg ggactcccac ctctgaacag aatccggtaa gctatactca agggacggta
     4381 cgagttgcaa atcagcaaaa gactatgatt gatcttttta cacctcaaaa aaccgtttcg
     4441 gacattttac aggataatgt tgccaatacg gtagctgcaa aaatacaaaa caaccatatt
     4501 gacaacataa aaacggttat tggaatcaac gataaattca cctttatcaa tgatattttt
     4561 aaaggtgaaa tatcaaaata caatgaagca attgaccggt tgaactcctt taaagatttt
     4621 caagaagctc tcaattatat caaaagttcg ggctttgaaa ccggaacaac agaaaaccaa
     4681 attgcattag aaaaattaat caagctttta aagcgtaaat tttacgaatg ataaatctat
     4741 gtttaaaaaa aaatacaccg tttatgaaaa aaaacttact accactattt ttcattttga
     4801 tcgtatttag cttctcgtcc tgcacaaaga ttaaaagaga atattaccca ctaacttcaa
     4861 tcaacctgca gatgatagaa aacataactc ccgaggatag aagtatttat tttcattttt
     4921 acactatcga gctttattca tgtttaaact atcttattcg ttacgatttt tccagcgaca
     4981 atggtaattt aaccatcaac ctaagagatg ttgaaaaaaa tgatctgtgt atcaatggtc
     5041 aagggccggc aacagcaagt atgaaccttg gcaagtacga caatggcacc tatcaaatta
     5101 acttgaccgt tgcgggagaa acaaatactg gcgctttgga ggtttccgat ttgggctatg
     5161 ttttacattt ccaaacatta aacaaactaa atttaattgc tgataccatg tttcgaatac
     5221 ctgaaaacat cttttgggga tatgttgcct atccgaagga ggcatctgcc ggcatagcta
     5281 accaagtact cgattcactt gaaagccacg gcgcatcacc acataattta cagcatggct
     5341 attatggtta ttttaccata aaagagaatg gagaaataac tttgttagat aaatctgatg
     5401 caaacttttc gatcaatttt atttacaaac aagaagaccc tgatgacaac ataaaagacg
     5461 taatccgata ttttaataat caccatttaa acgaagttta tatttttgta cgcacatcaa
     5521 aaggactggt ctttaactct ccaatgaata tttagttttt aaaaaaataa tctgatacga
     5581 ttacgatttc atttttaatg tttcataaag gaatcaaaac attttacaat tgtctaatca
     5641 ttctaaacat ttgaattatt taatttttta ctcatgaaaa atggttttac actgttcttg
     5701 ttaacagtac tttttgcgac agggattaac ctaaatattt tttctgcaaa tccacaactg
     5761 atttccaatc gtttgaccga acaaatagaa acatctcgtg atggtgaatt aataaagata
     5821 aatatcatca tgcgagagaa gtttgatact tataatcttt tgcgtaatgt ggaagatatg
     5881 gaaaaagagc aacgacgcag ttatgttatt tctgttttaa aagagtttgc ttcactttcg
     5941 caaagaggca ttcttgaaga tctaaacatg ttgcaactca ctgatcaggt ttcggatgtc
     6001 aacgcacttt ggattatcaa tataataaat tgttatgcaa ctcccacagc tatttacaaa
     6061 ttagcggagc gagccgatat tgagctgatt gaccatgatt tttatcagat aattttagat
     6121 ccatcagaaa acaaagaggc ttattttgaa gagggcaacg ttaacaaccg cgagatcact
     6181 tggaacgtaa ataaggtaaa cgctccacaa gtatgggctt taggttatga tggcgagggc
     6241 gtaattgttg ctttaattga taccggtgtt aactataatc ataatgatct taaagaccat
     6301 gtatgggttc atcctgattt cccctatcac ggttataatt ttgcaaataa taataataac
     6361 cctatggatg ataatggtca tggtacacac tgtgctggta ctgttgcagg cgatggtagt
     6421 tctgggtctc aaacaggtgt tgctccggct gccacgatca tgtgtctaaa agtgttagac
     6481 tcttcgggag gcggtcaaca gagcagtata tggtcagcca ctgagtttgc tgttgagcac
     6541 ggtgcacacg tacttagttt gtcgcttgga tggctacacg cttgggggcc taatcgtcaa
     6601 ctcttccgtc aagcctttga caatgtcctt gctgccggag tcgtttcatc agttgctgca
     6661 ggaaatgaag gtgagcaaca gggactttat cccgtaccgg ataacattag aactccaggt
     6721 gattgccctc ccccttggct tcatcccgat caaactttaa caggagagct ttcaagtgta
     6781 atttgcgtcg gcgcaacgaa tagcggcgat ggagttgccg gtttttcagg tcgtgggcca
     6841 tgcacatggc agtccgtttc accttataac gattatccat atcctagcga tataggatta
     6901 atacgtccgg atatttcagc tccgggagtt aatataaaat cattagctca ttacagtaat
     6961 accggttacg aatcgggctg ggaaggaaca tcaatggcta ctccgtgtgc agccggaata
     7021 atggccctta tgttacataa aaaccctctc ctgacgccgg cacagataag ccagatactt
     7081 gaagagacaa cggttgtttt acaacccggc aaaaataaca attcaggctc tggacgtgtg
     7141 aacgcattag cagcaattga ggctacccca ggccctggcc cagcatacta ttcccacagt
     7201 attaatgatg aaaccggtaa caacaacgga caattagatc cgggcgaatc ggtaaaatta
     7261 actgttgcaa tggccaattt cagcgaaatg gtcgttgaca gcgtaaccgt aaacatttca
     7321 acacaatcgc cttatgttac tattactgat ggaaccgaag agtatggaaa ttttgattta
     7381 aacagtatca ttgagataga ggatgctttt gcgttcgatg ttgcaaatga tgttccgggt
     7441 ggggaagcta tcaaattttt ccttttagca gaaaatgatt atgatagttg ggagagtagt
     7501 tttactatta cagccaatgg agtaacaatt acaagtagta actttagcat caatgacttt
     7561 agtggtaacg gaaacggaaa tctcgatccc ggtgaaaatg ccgatattct tattcacatt
     7621 aacaatctag gccagataac cgcacctaat gtaatttgta ctctcacatc cacctcttca
     7681 gatatcacta ttaacaacgg atcgtttaca atacccgtta ttgaaccagg tcaatcgtct
     7741 caggctaatt tcagcgtatc ggtaagtcct actgcccaaa ttggttctat tgcagagttt
     7801 aaccttgaag ttgttacagg atattaccaa tatgatttct catttttccc aaaaattgga
     7861 ttaattgttg aagattttga aacaggtgat ttcacgcagt ttccatggat gtttgccggt
     7921 aatcagccat ggactgttac aaatacagga gcttatcaag gtgtttactg cgctaaatcg
     7981 ggaactatta ataacaacgc atcatcagaa atgaaaatta ccatagaggc ttctggcggc
     8041 gattcaatag tgttctatcg taaagtatcc tccgaagcta attatgatca tctcaggttt
     8101 tacattgaca acaccaaagt aggcgaatgg tcgggcgaga cggcatgggg aagggttgcc
     8161 ttccctgtta ccgccggaac taagactttt aggtgggttt atataaaaga catttttttc
     8221 tcctctggtc aggattgtgc ctgggttgat tatattgaat tcccaagcgt ggttgacaat
     8281 agtcttgcag taaatgcagg cgccgatgca acgatctgcg aaggaacatc gcatcagctt
     8341 gatgctaccg cacaaaatta taccagtctg ttatggtcaa cctcgggtac tggaacattc
     8401 agcgattcaa caatccttaa tccgatatat acccccagtg aagccgacat taatgccggc
     8461 gccattacac tctctcttac cgtgtataat gagggtggag aatcgctgac cgataacttg
     8521 atacttaatt tccttttgtt gccttctaca ccagaagcta tcacaggact ttcacaggtt
     8581 tgtatgggag aaaacgaaac atactccatt aatcaagtgg ctaacgcaga atactataac
     8641 tggatattaa cacctgcaaa tgcaggatca tttgccggca acggaacgga aatagtaatt
     8701 agcttctcca acaactatac cggcgctgca tcactgaaag tatgcggaat aaacaattgt
     8761 ggtataggta atttttctga agagtttaat attttagtag aggattgtac aggaataaac
     8821 gagttggata caagcggtaa attttttata actccaaatc caaatgacgg atcattcatg
     8881 attcatttga atgataatat ctcaagaaca aatatcttaa aattaatgaa taccaaagga
     8941 caagtgatct tacagctaca attagaagat tcggatcaaa ttcagatgaa tacaaaatca
     9001 cttgaaaatg gtatgtactt cattatgata gaaaatgaac aatcggtttc aatacaaaaa
     9061 ctgattatca ataaataatt aatctctttt tatgctattt caaaatttaa caaacttaag
     9121 gtattgcctt tcatgataag aatcgtttaa aagttaaaat cttgaagtag ctatttttta
     9181 aaaatagttt ttatgaaaaa agttcttctc accatcctaa ctttaatttt tgctgtaagc
     9241 cttaatgctg aaacagtaaa aaagaattac cacttttcta atcctacgtt tgaaaccata
     9301 aaaggttatc aacagattag tttcgatgga cttttattaa ccggacaagc cggcgagcct
     9361 actttgccat atcaatccgt tgtattgctt attccacctg gacatactgc acttaatatt
     9421 aagcttgatt ttggtgaaga gatagaaatg gaaggcgtat ataatatcta ccccatgcaa
     9481 tatgtacagc ctgtttctaa aggaaaatcg ggaatttttg cctttaaaga ggatgtttat
     9541 aaaaaggatg ccttttatcc tgaaaagatg gaaggggtaa tttcaacaca ttttatgaat
     9601 ggctttgctg ttgctatttc atcatttaca cctgtacgat ataatcctta taaaggaaaa
     9661 ttgtcctact ttgaagatgt aacagtaact atagaaactg cgcctgactc caaagctata
     9721 aaggcattag aaaatattaa tacttcaccg gccgtgcttc aacggcttgc attattagtg
     9781 gaaaatagtg aagctgccgt taattatcca aagcgcgaag ttcgtaacga cgactatgac
     9841 ctgctgatta tcacagcagc gcagttcgaa aacgcatttc aacctcttat ctccttttat
     9901 cagcccagag gaatacgcac caaagtagtc tctactcaaa ctatctactc aaccacttcc
     9961 ggccaagata atcaagaaaa gataagaaat tatgttattg atcaatatca aaacaatgga
    10021 atccagcatc tgactcttgc cggcgacgta gagcatgtgc cttaccgggg attttactgt
    10081 tccgtacaat catcatcagt ttatactgac aatgatatcc cctccgactt atacttttcg
    10141 gcgcttgacg gaacctggaa caataataac aacaaccttt ggggcgaaat tggtgaagac
    10201 gatctcttac ctgaaatagg agttggaagg atgagtttca gctctcagtc agaactgagc
    10261 gctatgttaa ataagatata taagtatgtc ggcgagccgg ttttaggaga acttcgcgat
    10321 cctcttctcg ccggtgagca tctgtacaac aacccgctta cttggggatg tgattatttg
    10381 gagttgctta ttggatatca tgacgataac ggatatatca ccaacggaat accaaccgac
    10441 cataacatta tgacgatgtg cgatgaaaac ggcacttggt cggctaacca gttaaaacaa
    10501 aagataaatc aaggaacatc atttatacat cattgcggcc atgcaaactc aaattatgta
    10561 atgaggtttt acaattctga tatcacaaat tcaaatttct cgcagttaaa tggtgaaatc
    10621 cacaatttta cccttgccta ttctcatggc tgtatctgtg gtgcatttga tgataatgac
    10681 tgcattgccg aacgaatgat caatatagat aatcttgctg tagcagtggt tatgaactct
    10741 cgttacggtt ggtttaatga aggacagacg gaaggcccct ctgctcacct tcaccgtgaa
    10801 tttgtagatg ctctttacac cacaaaagag cctcatatcg gcatgactca taccttatca
    10861 agaataaaaa ccgcaccatg ggtaaatgcg ccaggacagt gggaagaggg cgctttgcgc
    10921 tggtgttttt acgattgtaa cgtattagga gaatctgctt taagaatatg gactgatgaa
    10981 ccggtagata ttacagcctc ttaccaaaat gctttgccta ttggcgtacc ctctatccaa
    11041 atttcaatat caggaaatgg atctgttgaa ggtttggcct gcacattaat aaaggaaggg
    11101 gttacgcatg gaacagcaat aacaaactca tcgggcctgg ccgagataat tttcgacccg
    11161 ccaattactg aacttggcga agcaattatt tacgtttcag gatataattg cctattaaat
    11221 gaatttccac taatcgttat cccaaacgat ggcgcttatg taatttatga atcatcgctt
    11281 gtaaatgatt ctcagggtaa tggcaatgga ttaccggatt atactgagtc aattcttctg
    11341 acaacaactc taaaaaatgt tggttcttca caggctgaca acgttactgc atcactaacc
    11401 tcggctaatc cttacgttac tattactaat aacagcgcct attttggaac aattccaggg
    11461 caaggttcat taactattga agatgctttt gaatttgata tagcaggaaa tgttcccgat
    11521 caacaagcga ttctgtttaa cattgaagct gtagggcaga ataactggtc ttccggcttc
    11581 tccattatcg ctaatgctcc taatttagta aaagacgaca taataataaa cgatgcttca
    11641 aacggtaaca ataatggaat attagatccg ggtgaaacgg ctgatatggt aataacaatc
    11701 attaactcag gccatgctga ggcctactca gtaaaagcct cacttacttc agccgatcaa
    11761 tatataacag tgaacacaac aacaccccag agcttaggta atatgactcc ggagcagtct
    11821 attccagcaa ctttttccgt ttttgcctcg ccagatactc ccgctggtta taccgggcag
    11881 ataactttaa acataacagc acagcatgga gtttcgacta cccaaactat caatttaaat
    11941 tttaccgatt attgttatcc tacagccaac tgttcatttg gtgatggtat aacaggtttt
    12001 ggtttagctt cgattaacaa tatgaataat ggttgcagca ataatggcta tggaaatttt
    12061 acggatttgt caacagaact tgagcctgga caaacttatc aagtgtcgtt caaaaccggt
    12121 tatagcaatc aaaaagtttg cctttggatt gattttaata gcaacaagga atttgaacag
    12181 tcggaaagat tggttacaga cttttctttg agtaactcaa atcaagttta tacaaccacg
    12241 atcacaatcc ctgaatccat aaccggtgga attaaacgct tgcgtataag agccagatgg
    12301 atggatagcg cacaagatcc ttgcgccaat tttacttatg gcgaaactga agattatact
    12361 gtaattttcc cagccggtca aatgagtatc aacgcttggt gcactcccga tgagatttgc
    12421 ctatacgatt ctactcaact tatagtaaca gccaccggcg gaacgggcaa ctatacctat
    12481 ttatggacac cggctgccgg ccttagcgat ccaaccattc aaaacccaac agcatcaccg
    12541 tcagaaacta ctacctatac agtggaagtt aacgacggct cttcatcaat caacactgat
    12601 gtgttagtta tagtcaattc tttgccacca acacccacca tcgccttaac aggaaacaca
    12661 ctaacttcaa gttccccaac aggcaaccaa tggtattgta gccaaggggc catcgaagga
    12721 gctacaggac aatcatatac ctgtacatgg gaagatgttt atttcgtaaa agtaaccaat
    12781 gaaaacggtt gcgtttcgat gcaatcaaac agcatacatg ttgtaattta ttccatagat
    12841 gactttgaac atgaaacaga tttcaacatt tatccaaatc cttttaatga tgaggttttt
    12901 attaactcta aaaattcaag cgacaaagta ctatatgttt cggtatttaa taacctcgga
    12961 caagaagttt taaagacaaa aatcaattca aaatcaacct cttccgatga aataattaaa
    13021 atctcaactg ctgaactgga caatggcatg tactttttca aaatctactc cgaaaataga
    13081 gtaataataa taaaactgat acgcaacaga taaaacattg atttaaaagt aattttcatt
    13141 ttttctcaaa ctatatttta atacttgcat atatttgtat tttcttttaa tgaactaaca
    13201 aaacaattat caccatgaaa aaacttatat tttccattct ggctatattg ttatcattta
    13261 tctcttttag tcagaaaagc tgggtacccc ttacatctga gcagcccttg caaagtaata
    13321 tcgccatatt aagctccgat cgttcagggc ttttactcga agttactatt ccgggaatgt
    13381 tcagcgaagt gataacacac gaagagatga cttttcaacg tatcacgctc gatgacaaca
    13441 ggacaaccaa agaggtcgga cggcctgagt taccaatgct tcacaagctg ataggtattc
    13501 ctggaaatca aaaaatcaga gtttcagtgt tatcaatgga gactaaaaag ctacaaggtt
    13561 attttatcta tccttttcaa acgcccacta ccgacaatcc cggcggacat aataaaccat
    13621 ttgtgatgga caagtcattt taccagaaaa acgcagtcta tcctttagag aactttattg
    13681 ttagtcagcc tggcatttgg agggatgtaa aagttgctgg agtagaagtt accccattta
    13741 actacaaccc ggttacaaaa gaattacaaa tagtaactcg tgcaacccta aaagttgagt
    13801 tttatggcac agatgagcgc acgacattta acccaaaaat ggaattaacc ccaaaattct
    13861 atcagatgta tagcgctgcc attgaaaatt ttgatgattt aggttacaca ctcacttatc
    13921 gggataattc gaacaccaaa taccttatta tcactaacaa cgaagccgtt tctactatcc
    13981 agccgcttat tgattggaaa aatcagatgg gcataaagta gaattaaagc ttattgaacc
    14041 cggttttaat actccacagc actttaaaac ttacattact gaactttaca acaaccaggg
    14101 tcttgagtat atattaatgg ttggtgatgc ttatcctaat ggaggaaaca gcggcgggcc
    14161 taatgtggtt ccaatgtttt attgggcgcc aggcggcggt gaagacccat catactccga
    14221 cagttggtac acatgtatgg atggccccga tgatcactac gctgatctgg ctatcggacg
    14281 ttttgtgtac aatataaatc aactggcaca acttcaatta cagattcaaa aaacattgaa
    14341 tcactatatg aacccagatt tgtcaagcaa ctgggctgaa aacacaatac tcatagctca
    14401 taaagaaaat tatcctggga aatacaccca atgctgtgaa gagataagga cattccctta
    14461 tgcgttgcaa acgccaattt tcgaaaaagc ttacggcggc caaggctatt ctaatgccca
    14521 agtagtaaat tatgtaaata ctaccggttg cggcattttc aactaccgcg gccacggcag
    14581 tactaccgaa ctatggcaat ggacaacagt gagcccgatg agtttcacag ccacccaggt
    14641 tgcacagttg accaatccaa gcagattgtt cgtattcttt gatgtttgct gcgacaacat
    14701 ggatattgtc tcttatgccg gtgaatgtct ttgcgaatca tttatgaaac acagcggcgc
    14761 gtcagttgcc gtaaatggcg ctatcattcc atcatatacc atccccaacc atgattatga
    14821 taaagagatg tacaaagcag tctatcatga aggtattact aacataggat atgttacaaa
    14881 ctttgccaac cttacggttt taaatgtaca tggtaatatt ggccgttcaa atgtaagaac
    14941 atatttatgg ttaggcgatt cttctcttga accctggact aaactggcat ctgaaatgac
    15001 tgttacacat gacgatcagc tatttcttgg actttcggaa tatccagtac aggtgcttgc
    15061 aaatggaatt gctgtggaaa atgcgatggt ttgtgtttca aacgaagagg gtagtgtata
    15121 tgccgtcggt tatactgatg gaactggatt tgtcaacgta caatttgatt cgcctgtcga
    15181 atttcctggt gaagcaactg ttacagtaac aaaattcaat tgtctgcctt atcaagctac
    15241 aatacctata attccgcaag acggacctta tgtagtaaaa gattcctggt tgattaacga
    15301 taatgtaggc ggaaatggca acggactcat ggattatgct gaatcaattt tgctgacttt
    15361 aagtgtgaaa aatgtgggaa cagagcttgc acaaaacgta aatgtaacgc tatctaccac
    15421 agatccctac atcattatta ccaatgcaac tgcttcttac ggaaatatcg gaccaaacgc
    15481 atccgtatcc cttaccgatg cctttgcctt tgatgtcgcc gaaaatattc cggataatca
    15541 ttcggttctt tttgctgtaa cagctaccga tggcacgaat atatggaata gtaatattgt
    15601 aattaccggg cgtgcacctg ttcttgctta cgatagcttt accatacttg atccaaacgg
    15661 taataataat ggaaaaattg atccgggcga agaagctaca atagtagtta ctgtgaaaaa
    15721 taccggatca gccgatgcaa ccggcgtttt aggattatta tcaactaatg atgcctacct
    15781 acaggtaaca caaataggag ctgttgcaat tggaaattta acttcaggcg attcaggtac
    15841 cgcctttttt agcgtttatg cttcgcaaaa tgccccagca ggacattctg cagaattatc
    15901 aattaatatt accgctgacc ataacctctc agaaacagat aacttcaatg taataatcgg
    15961 acaaattccg gtagtcatca tctgccttga tcctaaccac aattcaggcc ctaaaattca
    16021 aacagcatta aatacacttg gtattacctc cgaatatgtt acttcaatcc ctacagatct
    16081 taatctattc tcatctgcat ttgtttgtct tggagtttat agtagcaatc acgttttaac
    16141 tacaccacaa ggtcagcagc ttgctgattt ccttaatgcc ggcggttcgc tttatatgga
    16201 aggaggcgat acctggtatt acaatcctaa gactcctgta catacaatgt ttaaaattaa
    16261 tggggtgggc gacgggagtg gtgatctttc cactataaac ggacagacag gaacctttac
    16321 tgaaggtatg tcgttcactt acaacggcga caataactgg attgatagaa ttgaacctat
    16381 tgcgccagcc gttaagatct ttcaaaatca atcgccttca tacggcactg ctgttgcata
    16441 cgatgccggc acttacaaaa ctataggagc ttcccatgag tttggaggat taaccgatgg
    16501 cgcatcaaca aaagttgcac tgatggaaga atatatgaac ttttttggat tgataccatc
    16561 aggtgttaca gcaaatttcg tggctgatca aaccgaaata tgtgtaaata accaagttca
    16621 atttaccagt aactctattg gctccataaa cgaatggttt tgggaattcc agggtggtac
    16681 gccagcaaca tctaccgagg aaaatccatt aatcacatat tattttaacg gaacttttaa
    16741 tgtttccctt acggtaacag gagaaaatgg atcaaacaca atgaccaaaa acaattatat
    16801 taaagtttca ccaccaccaa gcgccgctgc tactccaacg ggtgtaactg cgctttgtca
    16861 aaatgcagcc aatactacct atacaacaac tggcgctacc catgctacac aatatacctg
    16921 gacactcacg cctaccaatg ccggcgtcat ttcaggaaat ggattaacag ctgttgtaga
    16981 ttgggatgat aatttttatg gttctgccga aataatggtt tccggttcca ataattgtgg
    17041 tcaaggtgca aactcgccat cacttattat cactatagat ccgctaccat cgcctgctgg
    17101 cgaaatatcg ggtgacacta ttggttgtca gggagaaacc gttttatata ctgtcgaaac
    17161 tattgctaat tccgaatcct acaactgggt acttactccg tcatacgcag gtagtttaca
    17221 aataaacaac aacgaagtga acataacctt tagtgataaa tatctcggag atgcaacatt
    17281 aaaagtatgc ggtgtgaatg cttgtggaga aggagaattt tccaacttgt ttgatatttt
    17341 tatagaaaac tgcattggca taaatgaatt atatggtgaa aaagagatca cactctatcc
    17401 aaatccaagt aacggtactt ttacaattga atttaacctt cgggatatag tagagattac
    17461 cattatcaat acattaggta aagaggtgta caactccggc cagcttaccc tttccggcaa
    17521 aacatatcat aatattgaaa taaaactttc ggatggggtt tattatctaa aagctgaagg
    17581 accaaaaaca catttaacta agaaacttat gatagttaaa taattatgtt tatatcaaaa
    17641 acataacaac taacaggctg acgaataatt tttattgtca gcctgtttct tttaaaaata
    17701 ttttgaaaaa cactatttaa aaaaggatgt aatggttaga gaaacacaaa ttaggatagc
    17761 aaaaattagt aagcgccggc ttgtaacaat cttaattctt ttatcattaa tgccactatg
    17821 catggtcttt ccccaagata ttgcaataga ttttggcaaa gcaaagacag gggtaactat
    17881 taaagaagat agttttgatc atttgaatat tacccttcag ttcgaaggga tcacttcctt
    17941 tcatgtatca actgtaaaag gggattttaa tgagatcggc attcccggac tatattcaat
    18001 cggccgatta ggaacaccaa agttaccggc agtaaaaaaa ctgattgaaa ttcctcatgg
    18061 cgctgaattt tcggttgaaa taaaaaatta ttccgtgaaa gaatacaaac tttctgacta
    18121 tgacataata tatcctataa tgcctgttca gccgtccata cgaaaggatc aaaattcaga
    18181 agatatccct tttgagtttg acgaaaatat ttaccagagc gatggttttc ttgaacctca
    18241 gcttgccaca cttgaagagt taggtatttt gcgaggaata aggcttgcca ggcttacaat
    18301 agcgcccgtt agttataatc cggtaaaagg tgtagtcaaa gtggttaatg atgttgaagt
    18361 tgaaataaca tttcgcaata ccgaccctgc tttgcatcaa tatatcaagg cttccaccta
    18421 ctcgccctat tttgacgtcg tacaaaatac aatgttaaat cgccttgagc atggctatcc
    18481 ggctcatcca gatttaacta aatatcctgt taaatacctt attgtctctc ctcgaatgtt
    18541 tgaaaatgac cttcagccat tcatccagtg gaagaccaaa aaagggttta aagttattac
    18601 cggctatacc gatcagatcg gcacctcgta cagcgctatc agaagttggg ttcaatcaca
    18661 atataactcg ggaacgccta ctgatcctgc tccctcattt cttcttatcg taggcgacac
    18721 tccacaaatt ccatcaacta cagggtccgc ctcaggtaaa atgaccgatt tatattatgg
    18781 aagcgttgat ggagattact ttcctgatat gtattacggc aggttttcgg caactaattc
    18841 tgctcagtta gttgcccaaa tcaataaaac catctattac gagaagtatc aatttgccga
    18901 tcctacctat cttaataaca taacgctaat tgccggagca gattcatact ggaatccaaa
    18961 aatcggacag ccgtgcatac tttatggaac taccaactat ttcaatacag cacacggata
    19021 taatgctatt aatgtttacc tgaactcata ttcaggatgt tacgatcctt ccagaatcgc
    19081 cgttagcctt atcaactata cagctcactg cagcgaaaca tcatggggag accctatgtt
    19141 gagcaagagt atggttaata gttttgtgaa taatggtaaa tacccggtag ctattggtaa
    19201 ttgctgcctt tctgctaatt ttggaacatc cgaatgtatt ggcgaaacat ggcaaagagc
    19261 tgcaaacaaa ggatcagtag catacatcgg ctcttcgcca agcagttact ggtttgaaga
    19321 tttttattgg tctgttggcg catttccaat acaaggttac aataacggat atgtacctac
    19381 ctatgcggaa acaacatggg gagcctatga tgcccctttc gtaagtgatt acgtaacaac
    19441 aggatctatg gtatttgttg gaaatctcgc agttactgaa gtacatattc aggggtatcc
    19501 ttcacattca agcccgctct actactggca agcatataac gtattgggcg atccgtcgtt
    19561 agtaccattt tacacccaac ccgatcctaa cacagtaact catacgccgt ttttcccaat
    19621 cggtctcgac acttatgaag taaacgcttt accaggttct tatgtagcca tttccaagga
    19681 taatgtgttg catggcgctg gattagtagg agtaaacggt gtggctcaga ttatgatcaa
    19741 tccggttcta tctgccggaa tggtggatat tgttgttacc aaacctcaac atatcccata
    19801 tataacacaa gtgcctgcgg cagctttgca aggaccttat atagtacttg agagttttac
    19861 tattaatgat gagagtggta ataacaatgg aaaagcagat tatgatgaag atatctggtt
    19921 gaatttaact tttaaaaata taggagccga tccctcctcc gatttaacag taaccatttc
    19981 cggcgacgat gattatacaa ctattactag cctgctatcg cagagctttc aatctatccc
    20041 aaacggagaa atgtttacac ttaacaacgc tttcagtatt aagataagtt catatctacc
    20101 cgatcagcat ttaagtaatt ttcaacttca aatcactgat ggtaatggtg attggaacac
    20161 atcatttaaa ataacactaa atgctccaca gcttgaaata gttgatctta caattgacga
    20221 ccaaactaac ggtaatggaa acggacacct ggatcccggc gaaacaatca acttgaactt
    20281 tacattgagt aacaatggcg gtgcagatgc aaaaaatgta aatgcattat taactttcga
    20341 cccatcaata tttaatattg gatcagaaat tattatgccc ggaaatattg gatcgggaga
    20401 acagaaagag tggtttgtaa tcataaatgt tttgcaaaat gctcctaccg gtatttttac
    20461 tcacctccat cttatgctaa caacaggtca atattccgaa cttaaaacct ttgatctcac
    20521 cttaggagtt gataataaca tcgaagattt tgaaagcggt gatttctcaa tgtttccatg
    20581 gcaattttcc ggaaatgcaa attggactat cacaaatgtt tctccctacg aaggaaattt
    20641 ctgcgctaaa tcaggagcta tttcacataa caaaacttcg gtaatgcaag taactgttat
    20701 taacttactc ccgggtgaaa tgtctttttg gcacaaagtt tcatcggaaa ctaattatga
    20761 ctttttacgg ttttatattg atggtgtaca aaaggacacc tggtcgggaa acacttcgtg
    20821 gcaacaggct acctacccca taacagcagg ccagcataca tacaaatggg cttatacaaa
    20881 agatatctca gtaaattctg gtagcgactg cgcatgggtt gatcttatta ccttcccgat
    20941 aaatgccact gcaggagcgt tagaggttaa catacaagct aatcaaacca taattgaagc
    21001 aggtgatttt attcagtttt atgacatttc ttccggtttt ccacttaatt tcaattggaa
    21061 tttcgaagga ggaatacctg caacatcaac taataaaaat ccaatgataa cctacgagca
    21121 acccggaaat tacaatgtat cgctaacaat tagcaatgca aacgggcaag cctcaaaaac
    21181 ttttgaaaac tatatcgtcg taacgtcgtc aatcattagt caaacccttt atatccctgc
    21241 tggatggagc ggcatctcat ctaaccttat tccacagaat acaatagtcg gaaatatcct
    21301 tcagccaatc cttaacgacc ttattgtttt gcaaaatatg aatggtgtat tttatcctga
    21361 gatgggaatt aacaccatag gtttgtggga ctacaagcag ggttacaaaa taaaaaccaa
    21421 tgcggaaact caattaaaca taaccggttg gcaaaatgaa caacaaactt tagacttaat
    21481 agcaggttgg aacctgatac ctattatgag taatttaaat caaaatatag agttgctttt
    21541 cagtggcctg ccccagaatg ggttgctggt aaaagaggtg gcgggaactt cactctactg
    21601 gccagaaatg aatattaata ctttattgga acttgaaccc ggaaaagcat attttgtaaa
    21661 aatagtcaat cctgcaacaa tatattttat gaatgatcct ttgaaataaa tattaaacat
    21721 taatttatct aacaatctgc taaatctata gaaattagca caatacgttg aatgaatatg
    21781 aaaaaaatta gcttaaccct tcttttaata atttactttt tagtaactgc caaagctgaa
    21841 gatttcaaca ccacgtttac ctatctttct gaaacttcat caagagttga atttaaactc
    21901 gataattttt atcttcaaca aattaacttg ggcggaaccg attatacccg gatagatttt
    21961 gctggtagag taaccacgat ggacgaaggc tttgccgagc ttccttttgt caatgtcaac
    22021 ataagtctaa gtcctttaaa aaacctctca tacaaaatcg tctctacaaa atataccgac
    22081 tatctgctgg attttcctct tgtgccctca cgcggggtta tttatcgaaa ccaagaccca
    22141 tccactatcc catatcgaat tgcatcggaa tcattaatag atgattatta tcctgcacag
    22201 cttatcgaaa tgatggatcc ctacataatc aaagatgtaa gaggaacaac cttatatttc
    22261 tatccttttc gttataatgc cgttcaacgc attctgaggg tatatacaga gataattgta
    22321 caacttgatg aagatgacac cgatcctgta aacccgctac ttaacccttc aggaaggtat
    22381 ttcaccgaaa tggaagaact ttatcgttca gtttttatca attacgaaag tcctgttgac
    22441 taccccaata ttggcgaaac gggtgatatt ttagtcatta ccaccactcg ggacgaaacg
    22501 gcaatacagc cttatattaa ctggaaaaag gaaaagggct acaatgtgtg gaaagaggta
    22561 gtaactgccg gaactaatgt aaaaaatcta atcaagcaaa aatataatgc caatccggca
    22621 atcttatttg tacagcttgt tggcgactgg gctgatatca aatgcgacat tggcggggaa
    22681 gaaaatgcgc ccatggatcc catgttaggc tgtgttgtag gtacagataa cttccctgat
    22741 attgctatag ggcggttctc ggcaaattcg gacactcatg ttactataca ggttaacaaa
    22801 agcattaatt acgaaaaaaa tccgtcagga gtatggtacg acaaggcaat tagcgtggcc
    22861 agcaacgagg gcgccggaat tggagatgat ggagaaattg attatcaaca tactaatata
    22921 atatacaact acaaactaaa cccatttact tacaataccc actacactgt ttatgaaccc
    22981 tctggtacag cgacaatggt taaaaatgct atcgaaaacg gagccagcat tattaactat
    23041 tgtggacatg gctccaagac atcgtgggga acgaccggct tcaacaataa caatgtaaac
    23101 cagctaacaa acggcaataa gttgcctttt atattttcag tagcctgtct taacggcgct
    23161 tttcattccg gcgactgctt tgccgaagcg tggctacgca aaaataatgg tggcgccgta
    23221 tggactatta tgtccactgt gaatcaacca tggcagcctc caatgagagg tcaggattat
    23281 tttaatgata tccttacagg cggatataac tataacacaa atccgggaaa tggaataaac
    23341 accaccgaag gcaggacaat actcggctca atagcgctaa acgggttagt acttatgtac
    23401 actgaatctt ctaactcctc cgacctgaaa accttacaaa catggacaac ctttggtgat
    23461 gtcgccttac aagcaagaac catgactcct aaaactctta ctcttacaaa cacaaaccta
    23521 caagctggtt taccattcga aacgattgta aaagctaatg gcgctcctgt taaaaatgca
    23581 ttagtcgctt tatcgcaaaa tggaacgtac gtaaaagcat ataccaccca aaatggagcc
    23641 gtttccattc caaataattt tattcccggc gatgtattat tagtggttac cgcatttaac
    23701 tgcaacacta tatatcaaac tatacaatcg gcgcctgcag ccggtccggc agtttattta
    23761 gatgaagtaa ccatcaatga tacttatgga aataataatg gtcaactcga ttatggagaa
    23821 acggtaggat tgaatgtaag tttaaaaaat agcggtttag aattagccaa aaatgtaaat
    23881 gccactcttt cttgcaacga tgattatata acgatactga atggtaatac cttctttgga
    23941 aatatcggaa tcgaaaacac agtaaccgtt tccaatgcct ttaccataaa ggctgctcct
    24001 gaaacacccg acaatcacga agtgttgttt agcattatag ccacttcaga aggctcttcc
    24061 tgggaaagct tctttagcct tactgcctat tcagccgtat taaattatga tagccatact
    24121 atcagcgatc cgacaggtaa caacaa
//
LOCUS       DAOOAI010000023        23735 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_2788_length_23735_cov_5.471917, whole genome shotgun sequence.
ACCESSION   DAOOAI010000023 DAOOAI010000000
VERSION     DAOOAI010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23735)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23735)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23735
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2788_length_23735_cov_5.471917"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(204..2636)
                     /locus_tag="PK345_02900"
     CDS             complement(204..2636)
                     /locus_tag="PK345_02900"
                     /inference="COORDINATES: protein motif:HMM:NF039587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5916 domain-containing protein"
                     /protein_id="HPX34002.1"
                     /translation="MKILPKLFLFLLLILNFSAVAYGQYERKQVIAVKTDTPPLIDGN
                     LSDRVWEGVTAASDFFQYEPKNSGDAGLNTEVRFLYDNNALYIGAMMYDSLPEKITRE
                     LGFRDSDNLNTDYISIEINPFDDGLNGFIFMVSSSGVQVDKKITEEGEDVSWNAVWKS
                     NVAINDSGWSAEFSIPYSALRFSKEVKNGWGLNIWRIRRSTRETFTWNFVDKKIEGAQ
                     KQTGIIVGFEEVKPPTRLSFHPYVSGYVQKKASEPKWLYSHHYGMDFKYGLSESFTLD
                     MTLIPDFGQVQSDDQVYNLTPFEIYYEEKRPFFTEGTELFQKGNIFYSRRVGALPIGY
                     FAVDDSLKENEYIKINPTQTNLINATKLSGRTANGFGIGVFNAVSANTYATLSDSTGN
                     TRKIITQPVSNFNMIVLDQNLKNNSYISLYNTNVYKGANYYTANVSGVQFLLFNKPST
                     YSLFGRFNLSQQYNTEAKDELGIHYNLQFSKTKGQFRFNYTSEAKSDTYNPNDLGFIQ
                     SNNSTSNMLELNYNFYNPFWKLLRLNNSLNINYQTLYDFKKFTDFSLMGNSWATFKNH
                     LSVGFNFFIRPVDEYDYYESRTSGKPFIIPPEWQFKIILSPDYRKPFIVDFSANYYTI
                     PQHDNSSVILSLSPRMRINDRFTVRFKSSWNRQTNDYGYVTTINTTNSPIIIMGRRNL
                     ENVENIFQTSYIFSNHIALDFRLRHYWLGANYLQYFNLENNGRLGIIEHNENNDFEVD
                     IFNIDLLIRWEFSPGSELAFVWKNAIQSYFTDDFQYNYFRNLGRTLYSPSDNSLSIKL
                     LYYLDYLNLKNK"
     gene            complement(2640..3155)
                     /locus_tag="PK345_02905"
     CDS             complement(2640..3155)
                     /locus_tag="PK345_02905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HPX34003.1"
                     /translation="MNSRIEVIIGDITQLKTDAIVNAANRTLLGGGGVDGAIHRAAGR
                     QLYEECKTLGGCQTGEAKITKGYHLPAKYVIHTVGPIWRGGNANEAKLLASCYSESLS
                     LANAHNIESIAFPAISTGVYGYPKNEAATIAIKTVKDFIEKYPLPKKVIFCCFDNHDF
                     ALYNKLLQASI"
     gene            3372..5831
                     /locus_tag="PK345_02910"
     CDS             3372..5831
                     /locus_tag="PK345_02910"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPX34004.1"
                     /translation="MIKKIIFSLVIFISIELVAQRPGGSGGGILPEGIVKGVISDSHS
                     GKPMEYANIVLFNVRDSSMVTGGVTANDGSFELEKIPFGRFYLEIYFIGFNRLTIGDI
                     RVTPNNKLVNLGLIKLDPAMQSLEAVEVTAEKQHIEFQIDKKVVNVSQDLMAQGGTAV
                     NALENTPSVTVDVNGDVSLRGSSNFLVLVDGRPSVLQGSDALQQIPASNIDRIEIITN
                     PSAKFDPDGSAGIINVILKQKKESGVNGIFNLSVGTKKKFNTDFLLNYRQGKFNFFAG
                     AGYRDFNSGGRRKSIQEAYLNDTTRYLFSAGDRKMHRTGYNLRGGIEYFMTDKTYLSF
                     NGEYGEFDFSHGFTSHQEEYTSPESLKRNLLTQNNSTRERAYYRLSLNSQHKFDEKGQ
                     QIEVMVNYSDNTNNSFEDQSESLTDENWQIIDPNPYKIKTLEPGEGTNWRFKVDYVNP
                     LSEKAKLEAGYQARIEQSKDDYDFMFWDMLINEWIENPLYSNKMEFDNDIHSIYGIYS
                     DQTKKFGYQVGLRTEYNYRRIINLKSETPALIDRWDFFPTLHLSKTFDNNDQIQASYT
                     RRINRPRGWSLDPFVMYIDAYNRRRGNPNLKPELTDSYELAFQKRKWGSLFSFEAYYR
                     YTTDKITRIRTLESDGTFLYTFENVNNDYSLGTELMVNTSLWKWFELNVTGNLFHYRI
                     KGDLEGNMVDNESMNWNIRTNAMFKLPHDFRLQINTSYNGPSITAQGTMEGFFMSSIA
                     LRKELFDRKLSISASMQDILKTAKWENTSSGIGFYSKDIMKPDFRFLMFNLSYKINNY
                     KRHRFNDQPEMQNTDMNVDDF"
     gene            5992..7962
                     /gene="pulA"
                     /locus_tag="PK345_02915"
     CDS             5992..7962
                     /gene="pulA"
                     /locus_tag="PK345_02915"
                     /EC_number="3.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008760402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051060 - pullulanase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pullulanase"
                     /protein_id="HPX34005.1"
                     /translation="MLNISCKQRSPFDFDDYSSYPVYNGDDLEMIYTPSETQFRVWAP
                     TAEAVKVKIYENGIDGETVIEKKMKKSLQGTWIIRFEKDMKDLFYTFQIKYNDEWLKE
                     TPGIYAKAVGVNGKRAAIIDLKETNPVGWDTIERPKLDNYTDIILYEVQIRDISIHPQ
                     SGIRYKGKFLGLAEEGTTTPLGEKTGIDHLKELGITHVHLLPAFDFRSIDETKLDENH
                     YNWGYDPQNYNVPEGSFSTDPYDPKTRIREFKEMVAALHRNGLRVILDVVYNHTGDTE
                     YSNFNLLVPGYYYRHNTDGSWSNASGCGNETASERSMMRKFMIESLKYWVDEYKIDGF
                     RFDLMGIHDIETMNIISNELHAIEPTLFLYGEGWKAGDSPFPEDKLALKQFTYKLDKI
                     AAFSDDLRDGIKGSWSDHKGKGFVNGNFEMAESVKFGVVGATEHHQVNYSKVNYSRAP
                     WAGEPYQCINYVSCHDNHTLFDKLCITNPEATNNDLIKMHKLANTIVMTSQGVPFLHA
                     GVDFVRTKQRVENSYQSPDSINQIDWIRKYTYRKVFDYYRDLITLRKQYPVFKMTNTE
                     LINKHLNFLYTNHPGIVAFRINDFTEAEKWKSLIVVYNASQQSQSIKLPVAKWLEIFN
                     SDGLNRSGYRIINNNLVNVAAISAIVLEEINE"
     gene            8007..10046
                     /locus_tag="PK345_02920"
     CDS             8007..10046
                     /locus_tag="PK345_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004376089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPX34006.1"
                     /translation="MTKKNKSQKRLALVENDPWLESAEQNLIDRLNRYNYRLKEIINT
                     YGSLSSFADAHKYFGIHWDKNAKGWYFREWAPAAFDLFLFGDFNDWNRTSHPLTKKEN
                     GVWEIFLDEKRYKEKFVHKSKFKVLVHAANGWKERVPVWINRVVQDESTKDFAGQLWQ
                     PAKSFSWKDDNFKINEIGNLFIYECHVGMAQEKEGVGTYVEFVENILPRIKKSGYNCI
                     QLMAIAEHPYYGSFGYHVSNYFAPSSRFGTPEELKQLIKKAHSMGIAVIMDLVHSHTI
                     KNINEGLNEFDGTDHQYTHPGERGNHPNWDSKVFDYGKIEVIQFLLSNVKYWLEEFHF
                     DGFRFDGVTSMMYFHHGNTTFGEIEKYFTEGVEYDAITYLQLANTLVHKIKKDAITIA
                     EDVSGMPGLCRPIEEGGIGFDYRLAMGIPDFWIKTLKEKRDEEWWMDDFYHVLNDRLP
                     DVKTVAYAESHDQALVGDKTIAFWLMDKEMYYHMQIDDNNLIIERGIALHKMIRLITL
                     SLGGQAYLNFMGNEFGHPEWVDFPRQGNNWSYHYARRQWSLCDNPNLKYIFMYRFDVE
                     MIKMAKENELLSAGYGSKIANDEENKTLVFEKGGLMFVFNFHPHRSIFGYEFYIVEPG
                     HYKIILNSDSPEFGGHNRVDDSRVYTSMYDSLSNKHYLKVYIPNRTALVFKKEGK"
     gene            10185..10865
                     /locus_tag="PK345_02925"
     CDS             10185..10865
                     /locus_tag="PK345_02925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HPX34007.1"
                     /translation="MFTDVPPSYDFVNRVLTWRFDVIWRKKAASESLKNSPSKILDLC
                     TGTGDLALQIRKKTESGAKVFALDYSKPMLKLAITKANKKKFDDITFIHADVANIPFP
                     DSHFDSVGIAFAFRNLTFHNPDHDIFLKEIYRIIKPGGRFVIVETSQPKNKLLRSLFH
                     FYMKHITSPLGGFLSGNRGAYRYLAHSAINYWNDQEATDYLKSYGFSQVTTKMILGGI
                     SAIYVAIK"
     gene            10903..11445
                     /locus_tag="PK345_02930"
     CDS             10903..11445
                     /locus_tag="PK345_02930"
                     /inference="COORDINATES: protein motif:HMM:NF033205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IPExxxVDY family protein"
                     /protein_id="HPX34008.1"
                     /translation="MKKKKFVIDFDGHQDYTIFNITSSQPDFRIAYLLNKHLGLQLQR
                     DENMLVKKSSNSDPQQFSFFSYTRDMRTTYYLIHDLSDQEPLIKNFFLLIHGFLIELE
                     QNELVTTINKIPEILSVNQLNLIDKQSIRKSQNKTTELLNLILTELEYHILTIAKQKN
                     ETKIQLKQTDRERNKRLRSE"
     gene            complement(11461..12222)
                     /locus_tag="PK345_02935"
     CDS             complement(11461..12222)
                     /locus_tag="PK345_02935"
                     /inference="COORDINATES: protein motif:HMM:TIGR01352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015291 - secondary active transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB family protein"
                     /protein_id="HPX34009.1"
                     /translation="MEEQKNRIKGIVGTLIFHLAIIFILFLLALRTPLPLPGEEGVEV
                     SLGYAETGSGLVQPIELAPVQKAEHTKPNTQTSDILTQEVEEAPAIEDVKPKEVKKTV
                     EEEKPVKEDPVVNPRALYTPHQTDSKETGGQGDDNNPADQGKTTGSNQSTKPDGIGGL
                     GSGISFSLEGRGSIHLPKPAYNSQEQGRVVVRIWVNKEGKVERAVSGVQGTTITDQTL
                     IKLAQDAALKAVFNSDPNAADQQTGTITYNFIRLN"
     gene            complement(12231..12641)
                     /locus_tag="PK345_02940"
     CDS             complement(12231..12641)
                     /locus_tag="PK345_02940"
                     /inference="COORDINATES: protein motif:HMM:NF014525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPX34010.1"
                     /translation="MAIQTRNKRSAQFSMASMSDLVFLLLIFFMLTSTLVAPNAIKLL
                     LPQSSSKTMAKQTVTVYINDRFEYFIGEDQIAVDQMEQLIGEAMAGQVEGSVVLRADQ
                     SVPVQHIVNVIDAVNKINERYNTKHKVILATRPN"
     gene            complement(12654..13343)
                     /locus_tag="PK345_02945"
     CDS             complement(12654..13343)
                     /locus_tag="PK345_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019130986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPX34011.1"
                     /translation="MFSAILLQVNQAGQVIDESVGEISMSLWDLTLKGGWIMLILGIF
                     SVIAIFIFIERFLILKQASKEDQNFMDHIRSFIHDGKIDAALALCRRTQNPISRMIEK
                     GLLRIGKPLSDINAAIENVGKLEVSRLEKNIAGLATIAGAGPMLGFLGTVVGMVRAFY
                     DMSMAGNNIDIALLSTGIYQAMVTTVAGLIVGILAFICYNILVARVEKLVFILEARAS
                     EFMDVLHEPVE"
     gene            complement(13447..14427)
                     /locus_tag="PK345_02950"
     CDS             complement(13447..14427)
                     /locus_tag="PK345_02950"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HPX34012.1"
                     /translation="MKVLGMGNALVDMMIRIDDERIFREFGLLKGGMRLVDSALIKDV
                     LERLKSFPVEIATGGSAANTVHGLAKLGVKTSFIGKVASDDFGKFMMEDMLKNGVNPN
                     LLNGKAPTGIALTLITPDSERTFAVNLGCAIELLPEDINAEMFEGYNIFHIEGFLVQN
                     KELLEKAVRLAKKAGAKVSFDLASFDVVEENFIFLKNIVEKYVDILFANEDEATAFTG
                     LTPEKALNVISQTVPVTIIKIGEKGSLISDRKDVVRVDSIKVQAVDTTGAGDLYAAGF
                     LYGMINGLSMHTSGEIGSLLSGKVIEVIGAKMDDERWSNIYTELRKLMME"
     gene            14542..14615
                     /locus_tag="PK345_02955"
     tRNA            14542..14615
                     /locus_tag="PK345_02955"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14576..14578,aa:Asn,seq:gtt)
     gene            14891..16225
                     /gene="miaB"
                     /locus_tag="PK345_02960"
     CDS             14891..16225
                     /gene="miaB"
                     /locus_tag="PK345_02960"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HPX34013.1"
                     /translation="MKYHLITLGCQMNISDSERVRTIIEKEIGYVWTDSEQEANLLGI
                     LACSVRQKAIDKVYTRIQKWNKWKKQKNLITFISGCILPDDMKRFLKLFDIIFRMSDF
                     EKLPELIRQYGVVSPASLVGDLSGISKNENIDGFWRVNPKYSSKFEAFVPIQNGCNKF
                     CTFCAVPYTRGREVSRDSNEILEEVKRLVSEDYKSITLLGQNVNSYGLDKKGKEITFP
                     ELLKKIGEIGKESDKEFWVYFTSPHPRDMTAEVIEIISQYDCLAKQIHLPIQSGDDKV
                     LIKMNRKHPVEKYRQIVEVIRRLIPEATLFTDIIVGFTGETDEQFENTRKIMDEFRYN
                     MAYIAQYSQRPGAVSSRWIDDVPREVKKARFHQLSNDLRKYSAAHNRDMIGRNYKVLV
                     TGKDRKKGFLSALTEGKIVSRFQSDNDELIGEFVTIKIISSTDLSVEGELVN"
     gene            complement(16278..16646)
                     /locus_tag="PK345_02965"
     CDS             complement(16278..16646)
                     /locus_tag="PK345_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018339155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3127 domain-containing protein"
                     /protein_id="HPX34014.1"
                     /translation="MSFELKGRLIEKYDSVQVTDRFRKREFVIEYRESASSYDFAETI
                     KFQLTQERCDILDNFHIGEELKVSFNIRGRRWEKDGQVSYFNNLEAWKIEKVVAAPQE
                     IIPQHTIEEIPPEPTDDLPF"
     gene            complement(16671..17348)
                     /gene="aat"
                     /locus_tag="PK345_02970"
     CDS             complement(16671..17348)
                     /gene="aat"
                     /locus_tag="PK345_02970"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HPX34015.1"
                     /translation="MPVYELTDDLVFPHPKLAEPDGLLAVGGDLSVERLLLGYANGIF
                     PWYEKSSPILWWSPNPRMVLFPEKFKISNSLKQTIKKQKFKITFDCDFSAVIKGCAET
                     KRLEQHGTWIVPEIEQAYNRLHEAGFAHSVEAWYDNRLAGGLYGVSIGKAFFGESMFY
                     KVRDASKVALAFLVEKLLGWHFHFIDAQQETAHMISLGGELIDIDHFLNLLKNALRYP
                     TLKGNWS"
     gene            17669..18493
                     /locus_tag="PK345_02975"
     CDS             17669..18493
                     /locus_tag="PK345_02975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34016.1"
                     /translation="MKSFLPFLLLLFFFPNVLSAHINFPEGGRSAAMGNASVALADFW
                     SIHNNQAGLAFYNKTSAGFYFENRFLVKEMSLKAGGATLPVGSGVFGFELVRFGNELY
                     NESKFGLAYSRKFGDYFAAGLQLDYLHKSLGNDYGKKGVATFEAGLMTKIKPDITLGM
                     HVFNPIHTKLTKYADERIPAVMRIGAAWSFDKSIILSVEAKKETSFDPSLKLGLEYRI
                     IDEVYVRGGLSSNPGQYTFGFGINLNRFTIDFASSVHNVLGFSPQFSAVYNFKTKR"
     gene            18490..20622
                     /locus_tag="PK345_02980"
     CDS             18490..20622
                     /locus_tag="PK345_02980"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPX34017.1"
                     /translation="MKTIPPHHIIKHKSVADRFCLIIAMLIMMIDVSLAQQLPVSPEY
                     MELLNSKLEEIAEQTEEQMDYTDLLEDIYYFLENPLNLNFASFTQMKKLIFLSELQIH
                     KIIEYRNVYGPFVTLYELMAIDGFDQELISFLEPLVSVDPEKPRISFKMKNIVKYGRN
                     DLFLRYSRVLEKQAGYANVSDSMKYANPGSYFLGNPDKYYLRYGFNYFNKIRLGITAE
                     KDPGEEFFKGSQPNGFDFYSGFVSVSEMRALEQLIIGDYQLEFGQGLTLWSGLSFGKS
                     PDAIGVIKNQRRVRPNTSVNENRYMRGTAVTFSLHNFKISGFFSSKKIDGNIATKDSI
                     SQEEQFVTSLQETGYHRTPNEIDKKNTVHETLYGANVRYRKGKFQIGATSFKALFDKP
                     LQLPDQLYRKYNFQGKENFNYGVDLNFIVKRFNIFAEASGSENGGKAYLAGFQASLHP
                     SVTLSAYYRNFGIKYQNLYSNAIAENTNNQNEKGMFAGLLVYLHRNWTFRGYSDLFSS
                     PWLRYRIDSPSRGAEYLAQIDYHPYKRMEMYLRYRYKQKQLNETENLIMAKTGETHKE
                     SIRFFISYKASSTVTLKNRIEFLTYDKPEATTQNGFIIYQDINYNPAAKPYNVSLRYA
                     LFDTDSYDERIYAYENDLLYVFSIPAYYYKGSRFYVMLKYEISENIDFWLRLSQTYYS
                     NKTTISNGTTKIDGNTYSEIKAQLKIRF"
     gene            complement(20948..22432)
                     /locus_tag="PK345_02985"
     CDS             complement(20948..22432)
                     /locus_tag="PK345_02985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34018.1"
                     /translation="MRIFQLILTTTLIVFADYLFAQHTFEKTFSRPEDQIINNVIEDN
                     DGNYIMAGRIKDTESTFYSGYIIKVDTTGNLLQEEIISPNDSISSLFFNIHLFNNHFY
                     ILGSQMVSYPDTSKLWYLKLNSNLEIEDEKLLNIPIGRWFSYMNSIIDSDTNIVITGY
                     TTRFDTVSPSPYNNDPFFFKISILGDSLSSNFISNDYHLSLSYDIIEKVDNSGYFSFG
                     FKYSSSISAGSQRYELTKQFDSIEILSVPYNINGYVSSSILNDSIIVLCGSGGSEPAP
                     EYSLNVLSTTFNNTPLNYNYFKMEDAVRDYSGYYQNIDIFDNSIYISGTSNFDYVNPF
                     WSTFNSWYHLVKLNSDLSSIWEYWYGGDAYYFLYGILATNDGGCIMVGNRYDYETQNQ
                     ERDIYIVKVDSNGLVTWSQEIPVNSLSTMVYPNPGTGQLNIKTSINNVDFELINLNGQ
                     VVIRKRLDENQSDINTEILKSGIYFYRLIDKKNKTVETGKWIKY"
     gene            complement(22496..23503)
                     /locus_tag="PK345_02990"
     CDS             complement(22496..23503)
                     /locus_tag="PK345_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34019.1"
                     /translation="MKWKNIFTYFGLATLITTSLIFYSCQKENSGIDKSVNQTEQSLK
                     DSQLEKRIIAFRDKIDLIRENPTLKSGSDPMDVDSAIWYIEATSNLTYGDASFKTEEF
                     IVDSAFIEVSVTNGQILWADVQAAYDQVIDILSEHNANIIANQKQLVVADIFLKKSND
                     NSATFEVTSGFGTDGTTGFGNDYSWYWGWELGRCDGSGLGVGKDAADKIAYLANMLIP
                     VPSGNSYYTDVSYEWISYWDVPTNNNPYRDYLLFHDYQEVTLNHACLSTNEITYYKNA
                     LITIGNMYKPTGKSTIRYFLWDDTAFGITPNWDDYWDMAHFAKVKYAIWHISNDPPSE
                     L"
BASE COUNT         7554 a         4525 c         4345 g         7311 t
ORIGIN      
        1 tcgaacccgc gaccccgtgc gtgacaggca cgtattctaa ccaactgaac taccggacca
       61 ataaagaact cttaaaagtg cggctgcaaa aatacatcaa cttttgtttc aaacaacatt
      121 tttttgcaaa aaagtaattt tattgaataa atcagaatta tttcaataag ctaatcaatt
      181 gtcaagttga tgctatttga tgattacttg ttttttaaat tcagataatc aaggtaataa
      241 agaagtttta tggaaaggct gttgtcagaa ggagaataga gtgtgcggcc caggtttctg
      301 aaataattat attgaaaatc atccgtgaaa taggattgaa tagcattttt ccatacaaat
      361 gcaagctcac tgccaggcga aaattcccat cggattaata gatcaatatt aaagatatct
      421 acttcaaaat cattattttc attatgttca attatgccaa gcctgccgtt gttttcaagg
      481 ttgaagtatt gcaggtaatt tgctccaagc caatagtgtc gtaacctgaa atccaaggct
      541 atatgattgg aaaatatata agatgtctga aatatgtttt caacattttc taaatttcta
      601 cgacccatta ttatgatcgg cgagttggta gtgttaattg tagtaacata accataatca
      661 tttgtctgac ggttccagct acttttaaat cgaacggtga atcggtcatt aatcctcatc
      721 cttgggctta atgataatat gacggaagag ttgtcgtgct gtgggattgt ataataatta
      781 gctgaaaagt caacgataaa tggcttgcga taatctggcg acaaaatgat tttgaactgc
      841 cattcggggg ggatgatgaa gggttttcct gatgtgcgcg attcataata atcatactca
      901 tccacaggcc gtatgaagaa gttaaacccg acactgagat gatttttaaa ggtggcccat
      961 gagtttccca tcaaggaaaa atcagtaaat tttttaaaat catagagagt ctgatagtta
     1021 atgttcaggc tattattcaa tctcagtaac ttccaaaacg gattataaaa gttgtagttc
     1081 aattcgagca tgtttgaagt agagttgttc gactgtataa agcctaaatc attaggattg
     1141 taagtgtcac tttttgcctc actggtatag ttgaacctga actgtccttt tgttttgcta
     1201 aattgaaggt tgtagtgtat tccaagttca tcttttgctt ccgtattgta ttgctggctt
     1261 aggttaaaac gaccaaagag ggaatatgtg gagggtttgt tgaaaagtaa aaactgaacg
     1321 ccactaacat tagcggtgta gtagttcgct cctttgtaca catttgtatt ataaagactt
     1381 atgtagctat tgtttttaag attttgatcc aatactatca tattgaaatt ggaaaccggc
     1441 tgagtaataa ttttacgtgt atttcctgtt gagtcggaaa gggtagcgta agtatttgca
     1501 gaaactgcat taaaaacacc aattccaaat ccgttcgcag ttcgacctga taattttgta
     1561 gcgttgataa ggttagtttg cgtgggattt atttttatgt attcgttttc tttaaggcta
     1621 tcatcaacag caaaataacc tattggcaat gctcccaccc ttcgcgagta aaagatgttt
     1681 cccttttgaa acagctccgt accctcagta aaaaagggac gtttttcttc gtaatagatt
     1741 tcaaatggag ttagattata cacttgatca tcggattgca cttgtccaaa gtcgggaatc
     1801 aaagtcatat cgagtgtgaa gctctccgat aacccatatt taaaatccat cccataatga
     1861 tggctataga gccattttgg ttctgaagcc tttttttgta catatcctga aacataggga
     1921 tgaaacgaaa gtcttgttgg tggctttacc tcttcaaatc caactattat tccagtttgc
     1981 ttttgcgccc cctctatctt tttatctaca aaattccacg taaaagtctc acgtgtacta
     2041 cgtctgatcc tccagatgtt taatccccag ccatttttta cctctttgga gaatcgtagc
     2101 gctgaatagg gaatcgaaaa ctctgcagac catccggaat catttatagc tacgttactt
     2161 ttccaaacgg cattccatga tacatcttcg ccctcttcag ttattttttt atctacctga
     2221 acacctgaac tggagaccat aaaaatgaat ccattcaacc cgtcgtcaaa aggattaatc
     2281 tcaatactaa tataatcggt attcaaatta tcagaatctc tgaaccctaa ttcccgagtt
     2341 attttttccg gaaggctatc atacatcatt gcaccgatat ataaagcatt gttatcatac
     2401 agaaaacgca cttctgtgtt taatcctgca tcaccgctat tcttaggttc atattgaaag
     2461 aaatccgatg ccgctgtaac accttcccat acacgatcgg aaagattccc atcaatcagg
     2521 ggaggagtat ctgtttttac ggctattacc tgcttacgtt catattggcc gtaagcaacc
     2581 gcactgaagt tgagaatcag caggagaaaa agaaaaagtt ttggtaatat cttcactttt
     2641 taaatagagg cctgtaaaag tttattgtaa agggcaaagt cgtgattgtc aaaacaacag
     2701 aagataactt tttttggtaa aggatacttt tcaataaaat cttttacagt ctttatagct
     2761 atcgtagcag cttcattttt aggataaccg taaacaccgg tactgatagc tggaaaagca
     2821 atgctttcaa tattatgtgc atttgccaaa gagagacttt cggagtaaca tgaggccaga
     2881 agttttgctt cgtttgcatt gcctccccgc catatcggcc ctacagtatg aataacatat
     2941 tttgccggca ggtgatatcc ttttgtgatc tttgcttccc ctgtctgaca accgcctaat
     3001 gtcttacact cctcgtaaag ctgacgccct gctgccctat gtattgcccc atccacacca
     3061 ccaccgccaa gcaatgtccg gtttgctgca tttacaattg catctgtttt taattgcgtt
     3121 atgtcgccta taataacttc gatcctacta ttcataaaat ttgattattg aaaaacaatt
     3181 tatttaaaga gaatgtaacc ttaaatctgt ttttttcctt tctgttcaga aaaaaagcaa
     3241 atgtagttaa ttcgttacta tttattacaa aatctggcat tatcggaata atacttttag
     3301 cctttttgtc taaaaacaaa gaagcaaaaa tcctgttatt ttttatataa attattttga
     3361 gttttatttt tatgattaaa aagatcatct tttcacttgt tatttttatt tcgatagagt
     3421 tggttgctca gcgtcccggc gggtcgggag gcggtatatt accagaagga attgtcaaag
     3481 gagtgatcag tgattcgcat tcagggaagc ccatggaata tgctaacata gtgcttttca
     3541 atgttcgtga ttcgagtatg gtaacaggtg gcgtaaccgc aaacgatgga agttttgaac
     3601 ttgaaaaaat tcctttcggc aggttttatt tagagatata tttcatagga tttaacagac
     3661 taaccattgg tgatattaga gttacaccca ataataaatt ggtaaatctt ggattaataa
     3721 aacttgaccc tgctatgcag agccttgaag ctgtagaagt tactgccgag aaacaacata
     3781 ttgaatttca gattgataaa aaggttgtta atgtcagtca ggatttgatg gcacaaggag
     3841 gaacggctgt aaatgcttta gaaaacacac cttcggtaac cgtggatgtg aatggagacg
     3901 ttagcttaag aggtagttcc aattttctcg ttttagttga tggccgccca agtgtacttc
     3961 agggtagtga tgccttgcaa caaattccgg caagcaacat tgaccggata gaaatcataa
     4021 caaatccttc agcgaagttc gatcccgatg gatctgccgg aatcataaac gtgatcttga
     4081 aacaaaaaaa ggaatctggc gtaaatggaa tttttaacct gtcggtgggt actaaaaaga
     4141 aatttaatac tgatttttta ctcaattacc gtcagggtaa gtttaatttc tttgctggtg
     4201 caggttatag agatttcaat tcgggcggaa gaaggaaatc tattcaggag gcttacctca
     4261 atgatacaac ccgttatctc ttttcggctg gcgataggaa aatgcatcgt acaggctata
     4321 accttagagg gggtatagag tatttcatga ccgacaaaac ctatttatca tttaatggtg
     4381 aatatggcga atttgacttt agccacggct ttacttcaca tcaagaagaa tatacatcgc
     4441 ctgaatcgtt gaagaggaac ttgctcactc aaaacaactc aacccgtgaa agagcatatt
     4501 atcgtctatc gttgaattcg caacataaat ttgatgaaaa aggtcagcag attgaggtta
     4561 tggtcaatta ctcggataat acgaataatt cttttgaaga tcaatcggaa tctttgactg
     4621 atgagaactg gcaaataatt gatcctaacc catataagat taaaacatta gaacctggcg
     4681 aaggtacaaa ttggcgtttc aaagtggatt atgtaaatcc gcttagcgaa aaggctaaat
     4741 tagaagccgg atatcaagcc cggatcgaac agagtaaaga cgattatgac tttatgtttt
     4801 gggatatgct cattaatgag tggatcgaaa accctctcta ttccaataaa atggagtttg
     4861 ataatgatat ccattccatt tatggaatct attcagatca gacaaagaaa ttcggttatc
     4921 aagttggcct ccgaaccgaa tataactaca gacggattat aaatctgaaa tcagaaactc
     4981 cagcccttat tgatcgttgg gatttctttc caacgcttca tctctcaaaa acttttgata
     5041 ataatgatca gattcaggca agttatacac gccggatcaa tcggcctcga ggctggtcgc
     5101 tcgatccttt cgtaatgtat attgatgctt acaaccgcag aagaggaaac cctaacctga
     5161 aacccgaact taccgactcg tatgagcttg cgtttcaaaa aagaaaatgg ggatcacttt
     5221 tctccttcga agcctactac cgatatacaa ccgacaaaat aacacgtatc aggactctgg
     5281 aatcggatgg aacttttcta tacacttttg aaaatgtgaa caacgactat tctctcggaa
     5341 ctgagttgat ggtaaacacc tccctttgga aatggtttga attgaacgtt accggaaatc
     5401 tttttcacta tcgcattaaa ggagaccttg aaggtaatat ggtggacaac gaaagcatga
     5461 attggaatat acgtactaat gcgatgttta agctgccaca cgatttccgc ctgcaaatca
     5521 ataccagcta taacggcccc agtattactg cacaagggac aatggaagga tttttcatgt
     5581 cgagcatagc gttgcgcaaa gagcttttcg atcgtaaatt atcaatatca gcatctatgc
     5641 aggatatttt gaaaaccgca aaatgggaaa acacctcgtc aggcatcggc ttctattcga
     5701 aagatataat gaaacccgat tttcgcttct taatgtttaa tttaagctac aagattaata
     5761 actacaaacg tcaccgtttc aacgatcagc ctgaaatgca aaatacggat atgaacgttg
     5821 atgattttta aaaaaaacgg ccttctttac cgttttactt tcagaaagtc aacccctcat
     5881 tttatcaatt tctgttatac aaagaagaat tagctaattt tgccgcaaaa tcttaatcag
     5941 tcggcagtgg ctaagaaaca tttaacatat ctgtttatac tcagtttcat tatgttaaat
     6001 atatcttgta aacaacgaag ccctttcgat tttgatgatt actcttccta tcccgtatat
     6061 aacggtgatg accttgaaat gatttacact ccttctgaaa cacagtttcg ggtatgggcg
     6121 ccaactgctg aagcagtaaa agttaagatt tatgaaaacg gcattgatgg cgagactgtt
     6181 attgagaaaa aaatgaagaa aagccttcaa ggtacatgga ttatcagatt cgaaaaggat
     6241 atgaaagatc ttttctatac ctttcagata aaatataatg atgagtggct gaaagagacg
     6301 ccgggaattt atgccaaagc ggttggagtt aatggaaaac gtgcagctat tattgatttg
     6361 aaggagacaa atcctgtagg atgggacacc atcgaaaggc ctaaattgga taactatact
     6421 gatatcatac tatatgaagt tcagatacgc gatatctcca ttcatcctca gtcaggaatt
     6481 cggtataaag gaaaattttt aggcctcgcc gaagaaggaa cgactacccc actgggagaa
     6541 aaaactggaa ttgatcatct taaagaatta ggcataacac acgtacatct tttaccggct
     6601 tttgactttc ggtcaattga tgaaacaaaa cttgatgaaa accattataa ttggggatac
     6661 gacccacaaa actacaatgt tcctgaaggt tcattttcca ccgatccgta cgatccgaaa
     6721 acgcgtatcc gtgaatttaa agagatggtt gcagctctac atcgcaacgg tcttagggtt
     6781 atccttgatg ttgtttacaa tcataccggc gatacagagt attccaattt caacctttta
     6841 gtcccaggtt attattacag acataatact gatgggtcat ggtcgaatgc atcaggctgc
     6901 ggtaacgaaa cagcgtcaga acgatcaatg atgcgtaagt ttatgataga atcgctaaaa
     6961 tattgggttg acgagtataa aattgatggc ttccggttcg acctgatggg gatacatgat
     7021 attgagacca tgaatattat cagcaatgaa ctacatgcca ttgagcctac tttatttctt
     7081 tatggagagg gctggaaagc tggcgacagc ccatttcctg aagataaact tgctctaaaa
     7141 cagtttacat ataaacttga taagattgca gcctttagcg acgaccttag agatggcata
     7201 aaaggatcgt ggagcgacca taaaggaaaa ggattcgtaa atggtaattt tgagatggct
     7261 gaaagtgtta agtttggagt agtgggcgca actgagcatc atcaggttaa ttattcaaag
     7321 gtgaattatt cgcgcgcgcc atgggcgggg gagccgtatc aatgcatcaa ctatgtctcg
     7381 tgtcacgata atcataccct gttcgataaa ctctgcatta ccaaccctga agctaccaac
     7441 aacgatctaa ttaaaatgca taagcttgcc aataccattg tgatgacatc acaaggtgtc
     7501 ccttttctgc atgccggcgt ggatttcgta cgtacaaaac agagggttga aaattcatac
     7561 caatctcctg actccattaa tcagattgac tggataagaa aatatacata ccgtaaggtg
     7621 tttgattatt atagggattt gataacactt cgaaagcagt accctgtttt taaaatgact
     7681 aatacagaac ttattaataa acatcttaat tttctctaca ctaatcatcc cggaatagtt
     7741 gctttcagaa taaacgattt tacggaagct gagaaatgga aatcgttgat tgttgtttac
     7801 aacgcatcac aacaaagtca atcaataaaa ctaccggtag ccaagtggtt agaaatattt
     7861 aattcggacg gactaaatag gtcgggttat cggatcataa ataataatct tgttaatgtt
     7921 gcggcaattt cagcgattgt actagaagaa attaatgagt aaaccttaaa accaatagat
     7981 ttaattacaa aagcatatca tcaaacatga caaaaaaaaa taaatcccaa aagcgtctag
     8041 cactcgtcga aaatgatcct tggctcgaat cggcagagca aaacttgatt gacagattaa
     8101 atcgttataa ctatcgtctt aaagagatta taaataccta tggaagtctt tcaagctttg
     8161 cagatgccca caaatatttt ggtattcatt gggataaaaa tgctaaaggt tggtatttta
     8221 gagagtgggc ccctgctgca ttcgatctgt ttttgtttgg tgactttaat gattggaatc
     8281 ggacttcaca tccattaaca aaaaaagaga atggggtgtg ggaaatattt cttgatgaga
     8341 agaggtataa agaaaaattc gttcataaga gcaaatttaa agtgctcgtt cacgctgcca
     8401 acggctggaa agagcgtgtg cccgtgtgga taaacagagt tgtacaagat gaaagcacaa
     8461 aggatttcgc cggtcaactt tggcagcctg ctaaatcgtt ttcatggaaa gatgataatt
     8521 tcaaaattaa cgaaatcggt aatctgttca tttatgagtg ccatgtaggt atggcacagg
     8581 aaaaggaagg agtgggcacc tatgtagagt ttgtcgaaaa tatcctacca cgcataaaaa
     8641 agtcgggata taactgcata caactgatgg ccatcgctga acatccttat tatggatcat
     8701 ttggatatca tgtatccaac tattttgcac cttcaagccg ttttgggaca cccgaagagt
     8761 taaaacaact gataaaaaaa gcacatagta tgggcattgc agtaattatg gatttagtgc
     8821 actctcatac aataaaaaac ataaacgaag ggctaaacga atttgatggt accgatcatc
     8881 aatatacaca tccgggagag cgcggaaacc atcctaactg ggattcaaaa gtctttgatt
     8941 atggcaaaat tgaggtaatt caatttttac tttccaacgt aaaatactgg ctcgaagagt
     9001 ttcatttcga tggattccgc tttgacggcg ttacctcaat gatgtatttt caccatggta
     9061 acacaacctt tggcgaaata gaaaaatact ttaccgaagg ggtcgaatat gatgctatta
     9121 cctatcttca acttgctaat acgttggtac ataagataaa aaaggatgca ataactattg
     9181 ctgaggatgt gagtggtatg ccagggcttt gccgacctat agaagagggt gggataggct
     9241 ttgactaccg gctggcaatg ggaatacctg atttctggat aaagaccttg aaagagaaaa
     9301 gagacgaaga gtggtggatg gacgactttt atcacgtact aaacgatcgc ttgcccgatg
     9361 tcaaaacagt tgcttatgcc gaatcgcacg accaagcgtt ggtaggtgat aaaactatag
     9421 cattttggct aatggataaa gagatgtatt atcacatgca aatagacgat aacaatctga
     9481 ttattgaacg cggaatagcg cttcacaaga tgatcaggct gataacgctc tcgcttggcg
     9541 gacaagccta cctaaacttt atgggaaacg agttcgggca tcccgagtgg gttgacttcc
     9601 caaggcaagg aaacaactgg agctatcatt atgctcgccg ccaatggtcg ctgtgcgaca
     9661 acccaaattt aaagtacatc ttcatgtaca gatttgatgt agagatgata aaaatggcta
     9721 aggaaaatga attgcttagc gccggatatg ggagcaaaat tgctaatgat gaagaaaata
     9781 aaaccttagt ttttgaaaaa ggcgggttaa tgttcgtttt caacttccac cctcaccgtt
     9841 caatatttgg ttatgaattc tatatcgtcg aaccggggca ctataaaata atccttaact
     9901 ccgactcacc ggaatttgga ggacataacc gtgtggacga tagcagggtt tacacctcaa
     9961 tgtacgattc gttatcaaac aaacattatc taaaggtata tatacctaac cgaacggcgc
    10021 tggtttttaa aaaagaggga aagtaacttt ttcatcatga ttttatatgc aattttatgc
    10081 cggccattac tttctatcta actccttaat caaaatttcc aactttaaat tcttaccata
    10141 ttatcgcaat aagttataat cccgataaac gtccgctgct taagatgttc acggatgtac
    10201 ccccatcata tgattttgta aatcgcgtgt taacatggcg ttttgatgtt atttggagaa
    10261 aaaaagccgc ctctgaatct ttaaaaaaca gcccctctaa aatacttgat ctctgtacag
    10321 gcacaggaga tttagctttg caaatacgaa aaaaaactga atccggcgcc aaagtctttg
    10381 cgctcgacta cagcaaacct atgctgaaac tggcaattac gaaagctaac aaaaagaaat
    10441 ttgatgatat aacattcatc catgccgatg ttgccaatat accgttcccc gactcccact
    10501 tcgattctgt aggtattgct ttcgcatttc gtaaccttac cttccataat cctgatcatg
    10561 acattttttt gaaagagatt tataggataa taaaaccagg tgggcgtttt gtgattgttg
    10621 aaacaagtca gccgaaaaat aagttgctac gctcactttt tcatttttat atgaaacata
    10681 ttacatcacc attaggcgga tttttatccg gaaatcgagg tgcttatcgt tatttagctc
    10741 actcagccat aaattactgg aatgatcaag aagcaaccga ttatttgaaa agttacggat
    10801 tttcgcaagt tacgactaag atgatattag gtggcatttc agcaatttat gttgcaataa
    10861 aataatttaa ttttgattat ccatgtataa aactacttgc aaatgaagaa aaaaaagttt
    10921 gttatagatt ttgatggaca tcaggattat accattttta atatcacctc atcccaacct
    10981 gattttagga tagcctattt actcaataaa catcttgggt tacaattgca gcgtgatgaa
    11041 aatatgcttg tcaaaaagag cagcaacagc gatccgcagc aattctcatt cttttcttac
    11101 acccgcgata tgcggacaac atattatctg atccacgatt tatctgacca ggagcctctg
    11161 atcaaaaatt tctttttact aattcacggc tttttaattg agcttgaaca aaatgagctt
    11221 gtaacaacaa taaataagat acctgaaatc ctctctgtca accaattgaa tttaatagat
    11281 aaacaaagta tcagaaaatc tcaaaataaa actactgaat tgcttaatct tatactcacc
    11341 gagcttgaat atcatatcct tactattgca aaacaaaaaa acgaaacaaa aattcagtta
    11401 aaacagactg atagggaaag gaacaaaaga ttgcgatcag aataaattca atcttgaacg
    11461 ttagtttagt cgaataaaat tataggtaat agtacctgtt tgttgatcgg cagcattagg
    11521 gtcggaatta aaaacagcct tcaaagcagc atcttgagct aatttaatta atgtctgatc
    11581 agtaatcgtt gttccttgca ctcctgatac ggctctttca actttcccct ccttatttac
    11641 ccaaatccta actactaccc gtccttgctc ttgtgaatta taggcaggct ttggcaaatg
    11701 tattgatcct cgcccttcga ggctgaaaga tatcccacta ccaaggcctc ctattccatc
    11761 aggctttgta ctttggtttg aaccagttgt cttaccttga tcggcgggat tattatcatc
    11821 gccttgtcct cctgtttctt ttgaatcagt ttgatgtggt gtataaagag cgcgtggatt
    11881 tacaaccgga tcctctttga caggtttttc ttcctcaacc gtctttttaa cctctttagg
    11941 cttaacatct tcaatagctg gcgcttcctc aacttcctga gtcaaaatat cgcttgtttg
    12001 tgtattaggc ttcgtgtgtt cagctttttg tacaggagcc agctctattg gctgtactaa
    12061 tccggagccg gtttcggcat aacccaggct aacctcaaca ccctcttcgc cgggcaaagg
    12121 caatggagtg cgcaatgcta aaagaaaaag gataaaaatg atagcaagat gaaatatcaa
    12181 tgtgccaaca attcctttga ttctgttttt ttgctcctcc atttttatta ttaattaggc
    12241 cttgtggcta atattacctt gtgttttgtg ttgtaacgtt catttatctt atttaccgca
    12301 tcaatcacat ttacaatatg ctgtactggt accgactgat cggcgcgtaa cactacagaa
    12361 ccttcaacct gacctgccat agcctcaccg ataagctgct ccatctgatc aacagctatt
    12421 tgatcttcac ctataaagta ttcaaaacga tcgttgatat atacagttac agtttgttta
    12481 gccattgttt tactactgct ttgcggcagt aatagtttta ttgcgttggg cgcaacaagc
    12541 gttgaggtga gcatgaaaaa aatcaacaac aaaaatacca gatcagacat ggaagccatg
    12601 ctaaactgtg cactgcgctt atttcgtgtt tgaattgcca tgtttatcaa gctttattcg
    12661 accggttcat gcaaaacatc cataaactcg gaagcacgag cttcaagaat aaatacaagt
    12721 ttttcaactc ttgccacaag aatattataa caaatgaaag ccaaaatccc tacaataaga
    12781 ccggcaacag ttgtaaccat agcttggtaa atacctgtgg acaacagagc aatatctatg
    12841 ttattacctg ccattgacat gtcgtaaaag gctctaacca tccccactac tgtacccaaa
    12901 aagcccaaca tgggaccagc gccggcaata gtagccaaac ctgcaatatt cttttcaagc
    12961 ctcgaaactt ctaacttccc aacattttcg atggcagcat tgatatcgct gagtggtttc
    13021 ccgatacgta gtaaaccttt ttctatcatc cttgaaatcg ggttttgtgt tcgacggcaa
    13081 agagctaaag cagcatcaat ttttccatcg tgaataaaag agcgaatgtg atccataaaa
    13141 ttttgatcct cctttgaagc ttgtttgagg attaaaaatc gttcgataaa aataaaaata
    13201 gctatcactg aaaatattcc gagaatcaac attatccatc cgcccttgag tgttaagtcc
    13261 cacaatgaca tcgaaatttc tcctactgat tcatcaatta cctgaccggc ctgatttact
    13321 tgcaacaaaa ttgctgaaaa catattttat tatttgtgtt tatgtaaaat gtcttctttt
    13381 aacgtaatcg cagcatgttt attagtaatt tgctcttttt ttccaaaaac ctacccccct
    13441 tactatctat tccatcatca gcttacgaag ctcagtatag atattactcc acctttcatc
    13501 atccattttt gctccaatta cttctatcac tttacctgat agcagagatc caatttcccc
    13561 tgaggtgtgc attgacaaac cattaatcat cccataaagg aatccagctg cataaagatc
    13621 gccggcgcca gttgtatcaa cggcttgcac ctttattgaa tcaacacgta caacatcttt
    13681 cctatcgctg atcaaagacc ctttctctcc gatttttatt attgttacag gaacagtctg
    13741 cgaaattaca ttaagcgctt tttcaggtgt taagcctgta aaggcagttg cttcatcctc
    13801 attagcaaaa agaatatcaa cgtatttctc aacgatattt ttcagaaaaa taaagttctc
    13861 ttcaaccaca tcaaagcttg caagatcaaa cgagactttt gcacctgcct tttttgcaag
    13921 cctgactgct ttttcgagaa gctctttatt ctgaacgaga aatccttcaa tatgaaagat
    13981 gttgtaccct tcaaacattt cggcattaat atcttctggt aaaagctcaa tggcgcagcc
    14041 aagattaaca gcaaaagttc tttccgaatc aggtgttatc aatgtgagag caatacctgt
    14101 aggcgcttta ccattaagca gattggggtt tacgccattt ttcaacatat cctccatcat
    14161 aaatttgcca aaatcatctg atgccacctt gccaatgaaa ctggttttta cacctaattt
    14221 agccaaacca tgcactgtat tagcagccga accacctgtt gctatctcaa caggaaatga
    14281 ttttagtctt tcaagaacat ctttaataag agcggagtca acaagtcgca taccgccttt
    14341 taataatcca aactcgcgaa aaatcctctc atcatcaatc cttatcatca tatccacaag
    14401 cgcatttccc attccaagta ctttcatgat ttcagtttta atacaaaatg taaaaaattt
    14461 tgggcaaaat tattttaatt tagaaaaagt ctctattttt gcagccgttt tacttttgta
    14521 aagcgtttaa atttttctga ttcctcctta gctcagttgg ttagagcatc tgactgttaa
    14581 tcagagggtc gctggttcaa gtccagcagg gggagcttgg taaagaataa ggctggcaaa
    14641 caaggtcagc ctttttttat tctcaggcaa tttgtaattg taagcataag cctgccccga
    14701 aaagtaatct tggccacaag cccacgaata taattttttg ccttatcctc taaacataaa
    14761 aagcgtgctt aatgtttgtc ttatattaaa tttgtcattt tttaagtatt aaaccatcaa
    14821 tctttttaaa aagaacactt taaaaccaaa actctaaata agaattttgc aattgaaaaa
    14881 ttaattcttt atgaaatacc acctgataac tctgggttgc cagatgaata tatcagacag
    14941 tgagcgcgtc cgcacaatca tcgaaaagga aatcggatac gtttggaccg acagtgaaca
    15001 agaggcaaat ctgttgggaa tccttgcatg ctctgtaagg caaaaggcga ttgataaagt
    15061 ttacacacga attcaaaaat ggaacaaatg gaaaaaacag aaaaacctga taacatttat
    15121 ttccggttgt attttgcctg atgacatgaa aagatttctc aaacttttcg acatcatctt
    15181 tcgaatgagc gattttgaaa aattacctga gctgatccgg caatatggcg ttgtaagtcc
    15241 tgcatcgtta gtaggcgatc tttcgggcat ttccaaaaat gaaaatattg atggtttctg
    15301 gagagtcaat cctaaatata gttccaaatt cgaagccttt gttcctattc aaaatggctg
    15361 taacaagttt tgtacttttt gtgctgttcc atatacgcgt gggcgcgaag tttcacgcga
    15421 ctctaacgaa atattagaag aggtgaaaag attagtaagt gaagattata agtcaatcac
    15481 cttgttaggt caaaatgtaa attcttatgg attagataaa aaaggaaaag agattacttt
    15541 ccctgaattg ctaaaaaaga ttggggagat agggaaagag tcagataaag aattttgggt
    15601 ctattttacg tcgccacacc cgcgtgatat gacggcagag gtaatagaga taatatcgca
    15661 gtacgactgt ctggcaaaac aaattcactt gccgatccaa tctggtgatg ataaagtttt
    15721 gataaaaatg aaccgaaaac atccggtaga aaagtatcgt cagatagttg aggtaattcg
    15781 cagattgatc cctgaagcta ccctattcac cgatattatt gtaggattca caggtgagac
    15841 tgacgaacag tttgaaaata caaggaagat tatggatgaa ttcagatata atatggctta
    15901 catcgcacag tattcgcaac gtcccggagc ggtaagttcg cgctggatag atgatgtacc
    15961 gcgagaagtg aaaaaagccc gttttcatca gttaagtaat gatctgagaa aatattcggc
    16021 agcacacaac agagatatga ttggacgcaa ttacaaagta ctcgttaccg gaaaagaccg
    16081 caaaaaaggg ttcctctctg cccttaccga aggaaaaatc gtctcccgtt ttcaatcgga
    16141 taacgacgaa ctaataggag agtttgttac gataaaaatc atttcctcaa cagatttatc
    16201 agtagaagga gagttagtta attgattttt gctcacaaca aatgatcagc aaaaagcatt
    16261 tatgatatta aaacagctta aaaaggcaga tcgtcagtag gttcgggagg tatctcttcg
    16321 atagtatgtt gtggtattat ttcctgtggc gctgcaacta ctttttcaat tttccatgct
    16381 tcgaggttgt taaaatagct tacctgacca tctttctccc aacggcgacc tctgatatta
    16441 aaagagactt tcagctcttc gccaatgtga aaattatcca aaatatcaca tcgctcctgg
    16501 gtaagttgaa atttaatggt ttcagcaaaa tcgtaagacg atgcgctctc acgatattca
    16561 atcacaaatt cgcgttttct aaaacgatcg gttacctgca ccgaatcata tttttcaatt
    16621 agccttcctt ttaattcaaa actcatttct tttactttgt ttagtgttta ttaactccag
    16681 tttcctttta atgttggata tcttaaagcg ttttttagca gatttaaaaa gtgatcaata
    16741 tctatcaatt cgccgcctaa gctaatcatg tgagctgtct cctgctgggc atcaataaaa
    16801 tgaaaatgcc agcccaacaa tttctcaacc aaaaaagcaa gagctacttt agaagcatcg
    16861 cgaaccttat aaaacataga ctcgccaaaa aaggccttac ctatggagac tccataaagc
    16921 cctcccgcta acctgttatc gtaccaagcc tccaccgagt gtgcaaatcc ggcttcgtgc
    16981 aaacggttgt aggcctgctc tatttcgggt acaatccagg tgccgtgttg ttccaaccgt
    17041 tttgtctctg cacatccttt tataacggca gaaaaatcac aatcgaaagt aatcttgaat
    17101 ttctgctttt tgattgtctg tttcaaagaa ttggaaatct taaatttttc aggaaaaaga
    17161 accattcgcg ggtttggcga ccaccaaagt attggcgagc ttttctcata ccacggaaaa
    17221 ataccattgg cataacctaa aagaagcctt tcaacactca aatcccctcc aacagcaagt
    17281 aaaccatcag gttcagctaa ttttggatga ggaaagacca gatcgtcagt aagctcatac
    17341 accggcatca aacaagattt ttattgaaaa atttataaca acgtcatttt aatatctttt
    17401 tcttagtaca aagaaaagaa aacgataaca attagctgct cattaatcag attttttaac
    17461 tttgtgatgg tttgtatttt agtctttcat tttgttaaaa attataccga tcgggaatgt
    17521 gtaaaagttt aattttgtca ttattgcgta aggctttgtg cgtttagccc tacttattta
    17581 gtggcaggga acggtattaa ccaaagacta aaatattttt ctgtgcagat ttttatgttg
    17641 taaatgggat tcaattaaaa gtgaaactat gaaatctttt ttaccgtttc tgttactttt
    17701 atttttcttt ccaaatgtgt tgtcggcgca tatcaatttt ccggaaggag gtcgttcggc
    17761 agcaatggga aatgcttccg ttgcattagc tgacttctgg tctatacata acaatcaggc
    17821 cggccttgct ttttacaata agacctcagc agggttttac ttcgaaaaca ggtttttggt
    17881 taaagagatg agcctgaaag caggcggagc aacattgcct gttggcagcg gcgtatttgg
    17941 ctttgaatta gttcgcttcg gcaatgaatt atataacgaa agtaaatttg gccttgccta
    18001 ttcacgaaag tttggcgact attttgccgc agggttgcag ttggactatc tgcataaaag
    18061 cttgggtaat gattatggaa aaaaaggtgt agccactttt gaagccggct taatgacaaa
    18121 gatcaaacct gatattacct tgggtatgca tgtttttaat cctatccata ccaaactgac
    18181 aaaatatgcc gatgagcgaa tacctgccgt catgagaatt ggcgcagcat ggagttttga
    18241 taaaagtata atcctttcgg tagaagcaaa aaaagagaca tctttcgatc cgtcgttgaa
    18301 gcttggttta gaatatagga tcattgacga ggtatatgtt agaggaggac tctcttctaa
    18361 tcccggacaa tacacctttg gatttggaat aaacctcaac cggtttacaa ttgatttcgc
    18421 atcatcggta cacaatgtgc ttggcttttc tcctcagttt tcggctgtat ataacttcaa
    18481 aacaaagaga tgaaaaccat cccgcctcat catatcatca aacataaatc ggtcgcagac
    18541 aggttttgtt taataattgc aatgctaatt atgatgattg atgtcagcct tgcacagcag
    18601 ctcccggtat caccggaata tatggaatta cttaacagca aattggaaga aatcgcggag
    18661 cagaccgaag aacaaatgga ttataccgac ctgctcgaag atatttatta ttttcttgaa
    18721 aaccctctta atttgaattt tgcatctttt actcaaatga aaaagttgat ttttttgagt
    18781 gagctacaaa ttcataaaat catcgaatac cgaaatgttt acggaccttt tgtaacactt
    18841 tatgagttga tggctattga tggtttcgat caggagctaa tctcttttct tgagccgtta
    18901 gtttcagttg atccggaaaa gccacgcatc tcttttaaaa tgaaaaatat agttaaatac
    18961 ggtcggaatg atttattcct tcgctacagc agggtattag aaaaacaagc cggctatgca
    19021 aatgtttctg attcgatgaa atatgctaat ccgggtagtt attttcttgg aaatcctgat
    19081 aaatattatc tgcgttacgg gtttaattac ttcaataaaa ttaggcttgg catcactgcg
    19141 gaaaaggatc caggcgagga gttttttaaa ggatcgcaac ccaatgggtt tgatttttat
    19201 tccggatttg tttccgttag tgagatgaga gcgcttgaac agcttataat aggagattat
    19261 caactcgaat tcggacaggg attgacattg tggagcggcc tgtcgttcgg caaatcaccc
    19321 gatgctattg gtgtaataaa aaatcaacga agagtaagac ctaatacatc agtaaacgaa
    19381 aaccgttata tgcgtggcac ggctgttacc ttttccttgc acaactttaa aatatccgga
    19441 tttttttcct ccaaaaaaat agatggaaat atcgcaacta aagattctat cagtcaggag
    19501 gagcagtttg ttacctcttt acaagagacc ggatatcacc gtacccctaa cgagattgat
    19561 aaaaaaaaca ccgtccacga aacactttat ggcgcaaatg tcagatacag gaaaggcaag
    19621 tttcagattg gagccacttc ttttaaagct ttatttgaca agcccttgca gctacccgat
    19681 cagctttatc gcaaatataa ctttcaaggt aaagagaact ttaattatgg tgttgaccta
    19741 aattttattg ttaaaaggtt taacattttt gccgaagctt ccggcagtga gaatggcggc
    19801 aaagcctatt tagccggatt tcaggcatcg cttcatccct cagtaacgtt atcggcatat
    19861 taccgaaatt ttggaattaa atatcaaaac ctatacagca acgccattgc cgagaataca
    19921 aataatcaaa acgaaaaagg catgtttgcc ggtttactgg tctatttgca tcgaaactgg
    19981 accttcagag gttactccga tctgttttca tccccatggt tacgttacag gattgactcg
    20041 ccttcgcgag gcgctgaata ccttgcacaa attgattatc acccttacaa aagaatggaa
    20101 atgtatctcc ggtaccgtta caagcaaaaa caactaaacg aaaccgaaaa ccttattatg
    20161 gccaaaaccg gcgagacaca taaagagagc ataaggtttt ttatttcata caaagcatcc
    20221 tcaacagtta cacttaaaaa tagaattgag tttcttactt acgataaacc tgaagctact
    20281 acacaaaacg gattcatcat ttatcaggat ataaactaca accctgcagc aaaaccctat
    20341 aacgtatcat tacggtatgc gcttttcgat accgacagtt acgacgaaag gatatatgct
    20401 tacgaaaatg acctgcttta tgttttttca atccctgcct attactataa gggaagccgg
    20461 ttttatgtaa tgcttaaata cgaaatttcc gaaaatattg atttctggct acggctctcg
    20521 caaacctatt atagtaataa aaccaccatc agtaacggaa ctactaaaat tgacggaaac
    20581 acatatagcg agataaaagc tcagcttaaa atcagatttt aacccctttt ggatgttttt
    20641 tacccaatcc actaaaattt taccgcatag ccagcatact caaactaaaa atctggaggc
    20701 tttttaatat tcctgtcctc accgtaaatc ctaccaatat caatcctaaa cctcaaactg
    20761 caatggctgt tgcaggcaat tcacggggtg agttttcctg gttactccat acccactttg
    20821 gcttctaaac ttatactgat gacttacata cccttactga aagtgagttg ttaaggattg
    20881 actggtttaa aagtctgtcc tgctattctt gctaactttt gttataagca ttaagcccct
    20941 tcttcattta atatttaatc catttccccg tttcaactgt tttgtttttt ttgtcaatta
    21001 gtctatagaa gtaaatacct gatttaagta tttctgtatt aatatcactc tgattctcat
    21061 caagtctttt gcgaatgaca acttgtccgt tcaagttaat taattcaaag tcaacgttat
    21121 taattgaggt tttgatattt aattggccag ttccaggatt agggtaaacc attgtgctta
    21181 ggctattaac cggtatttct tgagaccatg taacaagtcc attgctatca acttttacaa
    21241 tgtatatgtc cctttcttga ttttgtgttt cgtaatcata cctgttgcct accataatac
    21301 atccgccatc atttgtcgct aaaatcccgt atagaaaata ataagcatca ccaccgtacc
    21361 agtactccca aatagaactt aaatcagagt ttagttttac taaatgatac catgaattaa
    21421 atgtactcca gaaaggattt acataatcaa aatttgatgt gccagaaata taaatactat
    21481 tatcaaaaat atcaatattt tgataatatc ctgaataatc tcgtacagca tcttccatct
    21541 taaagtaatt ataatttaaa ggagtattat taaaggttgt agatagcacg tttaaactgt
    21601 attctggagc tggctctgaa ccgccacttc cacataaaac aataatggaa tcatttaaaa
    21661 tagaggatga tacataacca ttaatgttat atgggacgct caatatttcg atgctgtcaa
    21721 attgtttggt caattcatat cgttgacttc cagcggatat ggaacttgaa tatttaaatc
    21781 caaatgaaaa ataacccgaa ttgtcaacct tttctattat gtcatatgat agtgaaagat
    21841 gataatcatt tgaaataaaa ttagaggata atgaatctcc caatattgat attttaaaaa
    21901 aaaatggatc attattatat ggagatggtg aaacagtatc aaaacgagtt gtatagccag
    21961 taataacgat attagtatcg gaatcaatga tagagttcat atatgaaaac cacctaccaa
    22021 ttggaatatt aagtaacttt tcatcctcaa tttccagatt tgagtttagt tttaagtacc
    22081 ataattttga tgtatcagga taactaacca tttgagaacc taaaatatag aaatgattgt
    22141 taaataaatg gatattaaaa aacagacttg aaatactgtc atttgggcta ataatttctt
    22201 cttgtaacaa atttcctgta gtgtcaactt ttataatata gccactataa aaagttgatt
    22261 cagtatcctt aattcgtcct gccataatat aattaccatc attgtcttca ataacattgt
    22321 ttattatttg gtcttccggt cttgaaaatg tcttttcaaa tgtatgctga gcaaatagat
    22381 aatcggcaaa gactatcagg gtagttgtta aaataagttg aaatattctc atgacaaata
    22441 acattaaaac tttctacaga atcaatcctg tagaaggttt ggtttaaatt attgattata
    22501 gttcagatgg aggatcattg cttatatgcc agatcgcata ttttaccttt gcaaaatgag
    22561 ccatatccca ataatcatcc caatttggag taatgccaaa ggctgtatca tcccataaaa
    22621 agtaccttat tgtactttta ccagtaggtt tgtacatatt cccaatcgta ataagagcat
    22681 tcttatagta tgttatttca ttagttgaca aacaagcatg atttaatgtt acttcctgat
    22741 aatcatgaaa tagaagatag tctctgtatg gattgttgtt agtgggaaca tcccagtaac
    22801 ttatccattc gtaactaaca tcggtataat aagaatttcc acttggtaca ggaatcaaca
    22861 tattggccaa ataggcaatt ttgtcagcag catccttacc aacaccaagc ccagagccat
    22921 cacatcttcc aagttcccag ccccagtacc atgagtaatc atttccaaaa ccagttgttc
    22981 catcagttcc gaaacctgat gtaacttcaa atgttgctga attatcgttt gattttttta
    23041 aaaatatatc tgcaacgact aattgctttt ggtttgcaat gatattggca ttatgttctg
    23101 acagaatgtc aataacctgg tcgtatgctg cctgaacatc agcccaaagt atttgaccgt
    23161 ttgtaacaga aacctctata aatgcagaat caacaatgaa ttcctctgtt ttaaaactgg
    23221 catcaccata agtaaggtta gatgttgctt caatgtacca aatggcactg tcaacatcca
    23281 ttgggtctga acccgacttt aaggttgggt tttcacggat aaggtcaatt ttgtctctaa
    23341 atgcaataat ccgtttttca agttgtgaat cttttaaaga ctgctcggtt tggtttacag
    23401 atttgtcaat tccagaattt tctttttggc aagaataaaa aatcaaactt gtcgtaatca
    23461 aagttgccaa accgaaataa gtaaatatat ttttccattt cactttttta aaattttagt
    23521 caatacttga tttaatgggt ttcaattgat atttaagggt taataaattc tccatctgtt
    23581 tttaaattgt cggaatttta caatttcaat cttctttcat tttacctcct ttcttttatt
    23641 tcttattgct tataacgttt ggcggtatgg ttagttgccg atttcgaaac aatttcctat
    23701 caagttacaa ctacttttat acgagatgaa atgct
//
LOCUS       DAOOAI010000024        23252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_2867_length_23252_cov_5.473466, whole genome shotgun sequence.
ACCESSION   DAOOAI010000024 DAOOAI010000000
VERSION     DAOOAI010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 23252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 23252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23252
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2867_length_23252_cov_5.473466"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="PK345_02995"
     CDS             complement(<1..782)
                     /locus_tag="PK345_02995"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HPX34020.1"
                     /translation="MTISRRDFLNLIQLFSVGSLIALTTNKLLAKTPKEKINIGIQLW
                     SVRNELENDLFVTLKALSDMGVTGIEPYNFDGKFYGIEPRKFKKMCDDINLKIYSTHT
                     PITIDNAPLYVEKALEAGLEYLILPSLMNRPRNSISDYQKVAEELNAIGEICLQHNII
                     FGYHNHSFEFIKIDGSIPYDILLKDTDPQLVSFQCDIYWLVKAGYNPADYFKKYPHRF
                     KSLHIKDMNHDGDSCIVGNGTIDFPKLLISPGLTGNELIIYE"
     gene            complement(804..1853)
                     /locus_tag="PK345_03000"
     CDS             complement(804..1853)
                     /locus_tag="PK345_03000"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPX34021.1"
                     /translation="MPPKKKIKTGIVGFGLSGRVFHAPFISIHNGFKLHSIVSSGNVA
                     KQNYPKAKVFNDFLSLINNPEIELVVICTPHLIHAEQAAMALEKGKHVVIEKPVAMNS
                     HDFYKVIEISKKYGKRIFPYHNRRWDGDFLTIRHIINEGYLGKIIEFESHFDRYTPLV
                     TRAEWRYSQENGGGTLFDLGPHLIDQAIALFGAPLSVFCLLYYQRKGSKVNDSFDLKL
                     LYHSHAVTLKASVFVKEPGPRYQVHGTSGSFIKYGIDSQEAALKAGKIPNSKNFGNDP
                     KKYWGILNSNAYSKETRIRYATLPGKYMSFYDDVYATINKQKEPEVTPEDALLNMKII
                     EAAMESAKERRIIEL"
     gene            complement(1883..3451)
                     /locus_tag="PK345_03005"
     CDS             complement(1883..3451)
                     /locus_tag="PK345_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPX34022.1"
                     /translation="MDSDNTISGSKQYSRREILAGLASIPVIGVIGYGLFQHINDRNK
                     KLAPLLDDISSILPKGISPVVEGETIRLGIVGTGSRGKYLMKALGFLHPSRLDQYIES
                     AKKSNEWAQYLNDFYAQEDLNIEITAICDVFDKNANWGVETAANIHRNGTGGKMGNAP
                     KRFLTYQELVASPDVDAVIIATPDHLHIPVALEAVKNNKHIYCEKPLSCTVEETFAIR
                     EAVKESEIIFQLGHQGRQTENYHIAKSLINIGVIGKISLIEVCTNRNDPNGAWVYEID
                     PDANENTIDWKQFIGPAPWHEFSLERFFRWRCWWDYSTGLSGDLFTHEYDALNQILGL
                     GIPHSATASGGIYYYKDGRTVPDVLNMAFEYPNRELTLLYSATLASEKERGKVIMGHD
                     AYMELNKTLLVVADPKSTRYKEKIEKGIIEPHKPIFSYVPGQEKVDAITTPTEKYFAG
                     RGLLYTYRDGKSFDTSHLHLREWLNCIRLNNGLQPSCDIDQAFEEAITAHMGTISYLQ
                     GRRTYWDADKQKII"
     gene            complement(3454..3927)
                     /locus_tag="PK345_03010"
     CDS             complement(3454..3927)
                     /locus_tag="PK345_03010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HPX34023.1"
                     /translation="MNYSKSQLISLVALRFIVGWHFLYEGLTKIFTPSWTSKAYLLDS
                     GGFASSFFVWIAKNQTLLTFNDTINAWVLTLIGLFLILGCFEHLATIMGMLLLSLYYL
                     SHPPFPGIDYMFPSDGSYFIINKTIVEIASLAVICCFPTAHIIGIKRFFNKNRRK"
     gene            complement(3973..6474)
                     /locus_tag="PK345_03015"
     CDS             complement(3973..6474)
                     /locus_tag="PK345_03015"
                     /inference="COORDINATES: protein motif:HMM:NF024777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-N-glycosidase F-related protein"
                     /protein_id="HPX34024.1"
                     /translation="MKKFYYFLIMMMISSSVLISLSANSQTNQYLHFDKIDDFVILND
                     ASQYFSGTTQLTMTGWFYCDELSYGQGYFGFRIGSGSAEFYIIQLNNGVLECRLKTTT
                     GLHEYVAPANTVIPQVWQHIAWVFDVNTIKLYVNGALKGSTAASGIFQGANVPFAIGK
                     STLAGFNFVFGGRIDEVSVWKKALSQSEIQDIIDNELTGTEQDLELYYKFNQGEPGGN
                     NTAITHLICEIGNGERDALLMNFALTGETSNFNGTLNPGYQAISFPQIPNHLTTDDPF
                     EIEATATSGLPVFFEVISGPATIEGNIVTLTGQAGEVSIKATQPGNSQFDPAVPIINT
                     FMVLDPEVYVPDIDPRHPLEGTVYMSTLSKLRLTALSTIGYPELFSVESVNFIVDGQN
                     IPANDPLDNGYYTAWWTPPAYGNYTISIISSNNYGAIATKNVDINIASNATNLEIVAA
                     EGVWLNPSTDTQTVEATLPSFLGAFDQVIGTLQVSCPPGGCGEWDKLASIDAKSLDGE
                     WFEIIRYITPYGVPCSHSIDLTDYISLLGGKVSIRFNCATLDNGYLYKLTLNYRAGAP
                     QYLYSTVNQIWKAPYPFGDYANLQPVPDQMFQFSPNTVAAKLKLVSTGHGWGNLNTGN
                     AAEFYDATHHIWVNGEQTFTQHNWSTCNPNPDGCQPQNGTWYHNRAGWCPGSIAPWFD
                     YDMSSFISGSNVTLKYVFYEQYVDYCHPNHPDCVTGVTCADCNDGYNPVLEVACNIIS
                     FSNEYFVGVNKEVMAKILVQPNPSSGLINIIGPGNNPATGSFIKILNPSGALMGNYSW
                     NGCSAQLDLSTLPKGLYIMIITTDGNYEVTKLVLY"
     gene            complement(6531..8171)
                     /locus_tag="PK345_03020"
     CDS             complement(6531..8171)
                     /locus_tag="PK345_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SusD/RagB family nutrient-binding outer membrane
                     lipoprotein"
                     /protein_id="HPX34025.1"
                     /translation="MKVNCFIIKNLLFPVFVFLLFIFTACTKDFEDINTNPHGFTTAS
                     DGSLFNSVISSLQPGWDEQFYIWNEILYKQSQLAALTKDAWGNFTIGTEDIWRNYYLT
                     LPEVRELEKRFETYETTPEVLNMKAMLKITLALKTFKVADLFGDIPFSEAGYGFHNVA
                     LIRPKYDTQRDIYLKLLDDLEWADNNITETATMAEPFVTFKAFDKLFNGDMLNWKKLA
                     NSLRLRYALRMSEKEPQVAGLVISDVIENNKPVFYGYDFITPKLEAAAIWPAVMGFKN
                     ESLNWSFREHNGLRLGSNIWHQLSLNDSTDGSGIFDPRAYIFFETDNFNKWTFFPQLP
                     SVATPSSGGIPYGGHRDQAGNFDIKGENCIYAPFNYFIVRDEDNMPIPIITGAEIHYI
                     KAEAYLRGIGVAMDMMKAEIEYLNGINASVEWWLKVAEGLKLPLSGMSFPEMIQIPSS
                     LSSSSVQMHFGFWNATSDDEKLQFIYTQRWLDAFRQPIEAYSLARRTLLTPHEGDPIN
                     HFRLPYPPSEAENNLKNYSEAVARQGGDTPDIKIWWMP"
     gene            complement(8286..11606)
                     /locus_tag="PK345_03025"
     CDS             complement(8286..11606)
                     /locus_tag="PK345_03025"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPX34026.1"
                     /translation="MRLKLFTLFTFLLINVFGFAQQKSVSGKVYSDQDDIPLIGVTVL
                     IKGTLTGTVTDVNGRFTLPSVEASTILVFSYIGFETVEIFVGEQQFIEIRMLTKYAEV
                     EEVVVTALGVSRQKREIGYSTEKIDEDVLIESRAPNILNAISGRSAGVQISQPDGIEG
                     GSTRIVIRGNNNIGADNQPLIVVDNVPMENVPGLSNIGRGVDWGSAINDINAFDIESM
                     TLMKGGAASALYGSRGANGVLFITTKRGQKQKGIGVTYNMDYRITHPYRYREVQNIYG
                     HGGPISLTPPVFPTSGDTLLYPGIYDNAQLVINQEGKTSTTAEEFGYYGSSVSWGPKM
                     EGQMIKWWDGKMRPYSPQPDNLKIPFSDGYTTTHNISATGGGDKGTMRVSITRQDNKP
                     IIENSEFNRTTLNLGATLDISSKLKTDLTLSFINYHRLNSPMLGEDWNSFSKGLLYSW
                     PRSYQAVDKMNYANPDGTQNPQDGYPFLYLSKTIWWDYYNNNTTLDRDKYTAALTLIY
                     DMTSWLQVLGRIGRDFTIEQYETRNKPIDALGLQNGYYSNNLARNVGDNYDFTMTAKK
                     DEILDSKLDLTFSMGTSRYNTENYYIGGRSGTWYYPNMYTFFNYTDNIYSTDANNNTI
                     VEQFGNTPGSMAPSEQIIRKRINSVYSFLNLAYDSYLFLELTGRNDWSSTLPKGNNSY
                     FYPSVSLSFIASEVIDFQKHINWLNFMKIRGGIAQTATDTDPYLMTFHYNTTLFGGQQ
                     ASNFPATIPPLNLKPQRVNSYEGGLNLGFFDNRIDMDFTYYYLYSFDQIIPNLPLPFS
                     SGATTIMTNEGVLTNKGIELIINTVPVRTKDWLVRSGINIARNRNKVVSLGGSADSYM
                     LADIWGLNGPAMILHEGDSYGTISGYDYIFDENGNRVVNEEGTKYLITDTRVPIGNAA
                     PHFIGGWNTMAAYKGFKFTMLIDTKWGGDIYCGSYVIGLQTGQSPETLKEREGDGLPY
                     TDPDGNTSNIGIILDGVHEDGTPNTTVVHYYYKYLPNAGGWGKFVSTPGILENTWVKM
                     RELSLAYTIPQKWLEKLKAFQNLTVSFTARDLFYIYTTLPDKINPEGIMGSGNAQGFE
                     WASFPGTRSFVFGLTASF"
     gene            complement(11997..13580)
                     /locus_tag="PK345_03030"
     CDS             complement(11997..13580)
                     /locus_tag="PK345_03030"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPX34027.1"
                     /translation="MILADYLQLLDFAPIALLVADEKDEILYANKIAEATFFQELKQK
                     EVKHIFSYSKAKWAEIMKTSKEKGSYSFNDSIKKLTGGSIDVNVTCKPIKFIDDQVFV
                     YYIQDVTDQQKIIKELYKEQQYTQTILDSIPALVYVKDLENRIVSVNRAFEETTKLTN
                     EFVQGRSLSELTSDQELAIDFWKDDLEVIKSDIPLRKLIKPLFNDPNRKFITDKIPYK
                     TYDGETVGIIGFSTEVTDRVNLENALLESEKNFRLLFNTSPEGAIISSIDGRIIMANA
                     TLCKMLHYTEEELTKLSLFDITPKNKREVEASTLQLSILFGDTIEPVERSYLRKDGSI
                     LPAMVKGWIIKNEIGTPTLIGAFIRDITNEKIAEKKKNEFYEKEIERLKNEIDSKNRE
                     MISKLAQISEKDGQLKKVLRNLDNVVQNPPASIINELENILEEAKKDNIEVLWPEIEL
                     TFEKINKSFFDNLYKKHPKLTKNEKKITALLRMNMSSREISRITHQNIRSVEMARTRL
                     RKKLNLKRSDNLVAFLSQF"
     gene            complement(13620..14948)
                     /gene="rseP"
                     /locus_tag="PK345_03035"
     CDS             complement(13620..14948)
                     /gene="rseP"
                     /locus_tag="PK345_03035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HPX34028.1"
                     /translation="MEIVTKISLFLLSLSILVIFHELGHFIAAKIFKTRVEKFYLFFN
                     PWFELFKFKIKDTEYGIGWLPLGGYVKISGMIDESMDKEQMKQPPKPYEFRSKPAWQR
                     LIIMLGGVLVNILLAIVIYIVMLYSWGEQYLPTAEVKYGITVDSLAYEMGLRNGDYIL
                     SIDNKEVEDFHKILPTIIIDEAKTIQVSRDGQMKDIKIPSGFLNKLIHHRSPDLIGIR
                     FPFVVGDFTKNSSAKEAGLQINDKIIAINDTQVNFFDEFRDEIQHFKGQNVNISVIRN
                     GDTLSNTVAVSPEGIVGIYPQNPSEYFNLKEKSYSLIQAIPAGTAKAFDEVANYLKQL
                     RLLFSKEVKAYESVGGFITIGSIFPSTWNWRAFWSLTAFLSIMLAILNLLPIPALDGG
                     HVMFLLYEIIVRRKPSDKFLEYAQIVGMLFLFGLLIFANGNDILRLIGSK"
     gene            complement(15163..16326)
                     /locus_tag="PK345_03040"
                     /pseudo
     CDS             complement(15163..16326)
                     /locus_tag="PK345_03040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004296793.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
     gene            complement(16342..17217)
                     /locus_tag="PK345_03045"
     CDS             complement(16342..17217)
                     /locus_tag="PK345_03045"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPX34029.1"
                     /translation="MKRRKKQTLGYSPKLLRKYRLVLMDVQTYEEKISLVITRLGVFV
                     VVGLTIVSLIVLTIYLVAFTSLREYIPGYTDLSLPKQIYNLQRKADSLEVELKHRQTY
                     MDNLKRILSDDDMYEQPTMSYTSTPYYDTITLRHSAADSALREEYESQTEFTLYPTNI
                     FTYPTLSIGNIYFFSPVNGIITRKYNPIQSHFGVDIVAAPNVPIKSVLDGTVIFSDWT
                     LETGYIICIQHDHQLVSVYKHNSSLLKQQGSKVKAGESISFIGQSGELQTGIHLHFEL
                     WYDGKPVDPEEFITF"
     gene            17426..20131
                     /locus_tag="PK345_03050"
     CDS             17426..20131
                     /locus_tag="PK345_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX34030.1"
                     /translation="MTKIKNTVFKGNLSHRYFSLFSFIAIVGSILLVISCSTKKNSFT
                     SRTYNNLVSHYNTYWNGRESFNEGVRNLERDAKDNYTTILPVFKFGNEQEAQAVAFNM
                     DRAIEKSSKVILRHSMYFQRKEYVRRVKDSYMLIGKSYLYKREYPNARRTFEWISGFY
                     KDEAVKYDADLWLAMTYNQTGEFEKSSMVLDKLNKDIRTGIAPAKTDRLFQMIYADYY
                     ILQNDYQPSIKYLKRAIELNSRKKDKNRLRFILAQVHQEIGDMKEAARLYQLLIRKNP
                     PFEMSVNAKINLAKTYDTKRSNRNMIVKTLEKLLKDHKNSEYRDQIYYALADMYAKDN
                     QTEKVIEYLALSVATSVSNDYQQTLSSLKLADIYFEQPDYIFAQAYYDTAMQVMPNNF
                     PNYKSIKEKTEVLTELITNLRNISIQDSLQNMAKMPEAERNKIIDEKIRKIVEEEKRL
                     AEEQREAQRTVSMLEQTNRMENRNLQQSGGWYFYNPSAMSFGYTEFINKWGRRKLEDL
                     WRLSNKQASIEFDELAEGEEGENGAENDSTLKLESDPKKREFYLQNLPLTEEAMEASQ
                     KIIENALFEAGYIYKDGLQDNEHSIQAFEELIEKFPEHENRLMVFYQLYKNWEEADDR
                     NKADYYKNLIIRDFPETDYAKILLDPNYNLVVQARLDAASNLYYDTYSAYMNNQFYLV
                     INNSNLAQEQYQDSELIAKFEFLKGLSFGKLQDVDTMRTTLEDLIANYPESSIKPLAQ
                     DILSRISQDETGQLVLHETSVNVEENEITGENPEEEFTSIYSANNSSVHFYGMIVDAS
                     QTNINALKVRLSDFNANNFRLSQLKINSIVFDNDLQLVTVGNFENAEKAMVYYNSVLN
                     NDYITSPLEGTDTSFFVITTDNYPLFYREKEIQGYMKFFERTYLE"
     gene            20211..20633
                     /locus_tag="PK345_03055"
     CDS             20211..20633
                     /locus_tag="PK345_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HPX34031.1"
                     /translation="MSKHSTNDISPSINFLANGTSMAGEIKSNGDFRIDGTLEGSISS
                     KGKIVVGSSGKVKGEIVCQNADISGDIKAKIVVHELLTLKSTAVLNGDIFTKKLAIEP
                     GAYFTGTCNMHDIPGNVKDEIQKKTEIPAPKEQTKVNV"
     gene            20746..21084
                     /locus_tag="PK345_03060"
     CDS             20746..21084
                     /locus_tag="PK345_03060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34032.1"
                     /translation="MLLMFALHLLSIVLFPSGPISRMTPYSILFFSIFNLLFFYIKRW
                     LYQSKDSKFINLFLIFNSVKMVLFIAIIAVYAYFFHDDAINFAIGFFICYAVFTTVLV
                     RSFNRLQKVR"
     gene            complement(21399..21776)
                     /locus_tag="PK345_03065"
     CDS             complement(21399..21776)
                     /locus_tag="PK345_03065"
                     /inference="COORDINATES: protein motif:HMM:NF019421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA domain-containing protein"
                     /protein_id="HPX34033.1"
                     /translation="MKVANGEKRILQSWQVKQKNTGANFKYEYSYEEIGLLLQKFVTA
                     WRVEIEKYFLLVLFNFLFSNGDAHLKIFSLLESSKGDYLLNPAYYLANTRLHVDDTDF
                     ALYKGPFVDDFKSDVFKKSNHVS"
     gene            complement(22212..22538)
                     /locus_tag="PK345_03070"
     CDS             complement(22212..22538)
                     /locus_tag="PK345_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HipA N-terminal domain-containing protein"
                     /protein_id="HPX34034.1"
                     /translation="MRRVQVYRNGILAGILTGEDCKYYVFRYDDSYFTNSDMPASSLT
                     LLKSQQEYKSKFLVPFFFNMLSEGVNKKPQCKQLRIDLEDNFGLLGATAMYDTIGAIT
                     IKPLSK"
     gene            complement(22551..22814)
                     /locus_tag="PK345_03075"
     CDS             complement(22551..22814)
                     /locus_tag="PK345_03075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015548023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPX34035.1"
                     /translation="MTLSFYGKQEQNQIKMLANHLGKTTRNRRKELGIAQPHLAELAK
                     VSTNTLCKLELGQGDPSLDVMKKLAEALGLELKIEVKKIVNGQ"
     gene            complement(22934..>23252)
                     /locus_tag="PK345_03080"
     CDS             complement(22934..>23252)
                     /locus_tag="PK345_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34036.1"
                     /translation="NLAVIILTLTNFGKARKRNYLLQITYLGDPETETQIERILKKHC
                     RKIKLVNLKNVGSDQVLEAFYHVSLKEEKKNSRLLSDLTASKLIHHVNLYFDEDDVNP
                     PTY"
BASE COUNT         7101 a         4472 c         4232 g         7447 t
ORIGIN      
        1 tgctcgtaaa taattaattc attgcccgtt aacccaggcg atattaataa tttcgggaaa
       61 tcaatagttc catttccaac aatgcaacta tctccatcat ggttcatatc tttaatatgt
      121 aatgatttaa agcgatgagg atattttttg aaataatctg ccggattata tcctgctttt
      181 actaaccaat agatatcgca ttgaaatgaa actaattgcg gatcagtatc ttttaaaagg
      241 atatcgtaag gaatagaccc gtcaattttt atgaactcaa aactatgatt atgataccca
      301 aaaatgatgt tgtgctgtaa gcaaatctca ccgatagcat ttagctcctc agctactttt
      361 tgataatcgg agatactatt acgtggtctg ttcattaacg agggcaggat aaggtattca
      421 agaccggcct ctaatgcctt ttcaacataa agaggtgcat tgtcaatagt aataggtgtg
      481 tgagtactat agatttttag gttaatatca tcgcacatct ttttaaattt gcgtggctca
      541 attccataaa attttccatc gaaattgtaa ggctcaattc ctgtaacccc catatcactt
      601 aatgctttca gcgtgacaaa aagatcattt tccaattcat ttctaacgct ccaaagttga
      661 atgccgatat tgattttctc cttaggtgtt ttggctaata gtttattggt tgttaaagca
      721 atgagggagc caaccgaaaa aagttgtatc aaatttagaa aatcacgtct cgaaattgtc
      781 atttctcttt gattttataa tggttaaagt tcaattatcc tacgttcttt agcgctctcc
      841 atggccgctt caataatttt catgttcagt aaagcatctt ctggagtaac ttcgggctcc
      901 ttttgtttat ttattgtagc ataaacatca tcataaaatg acatatactt tccaggcaaa
      961 gtagcatatc ttattctggt ttctttacta taggcatttg aatttaaaat tccccaatat
     1021 tttttcggat cattaccaaa atttttgctg ttgggtattt taccggcttt caatgcagcc
     1081 tcctggctgt ctattccgta ctttataaac gatcctgatg tgccatgcac ttgatatctt
     1141 ggtcctggtt ctttaacaaa cacacttgct ttgagtgtaa cagcatgact atgatatagc
     1201 agtttgagat cgaagctgtc gttaacctta ctccctttac gctggtagta cagaagacaa
     1261 aataccgata agggagcccc aaaaagagca atagcctgat caatcaggtg tggacctaga
     1321 tcgaacagag ttcctccgcc attttcctgc gagtatctcc attcagctct ggtaactaaa
     1381 ggagtatatc ggtcaaaatg cgattcaaat tcaattatct ttcctaaata accttcgtta
     1441 attatatgac gtatcgtcaa aaaatcgcca tcccatcggc gattgtggta tgggaatatc
     1501 cgtttcccat attttttaga aatctctatt actttgtaaa aatcgtggct attcattgca
     1561 acaggttttt ctatcaccac gtgtttgcct ttttcgagag ccatggcagc ctgttccgca
     1621 tgaatcaggt gaggagtaca aattactact aattcaattt caggattatt tatcaaactc
     1681 aagaaatcat taaacacttt agcttttgga taattttgtt ttgccacatt acctgatgac
     1741 acaattgaat gaagcttaaa tccattatga atggatataa agggagcatg aaacactctt
     1801 cctgacaatc caaatccaac aattcctgtt ttaattttct tttttggcgg cattaatttt
     1861 taatctttta gttaataaaa catcaaatga ttttttgctt atcagcatcc caatatgttc
     1921 gtcgcccttg gaggtatgaa atcgttccca tgtgtgccgt aatggcctct tcgaatgcct
     1981 gatcaatatc acagcttggt tgtaaaccat tgtttagtct tatacaattg agccattcgc
     2041 gtaggtgtag atgagaagtg tcgaaagatt tcccgtcgcg ataggtatag agaagcccgc
     2101 gaccggcgaa atatttttca gtaggcgtag tgatggcgtc cactttttct tgtccaggca
     2161 cataagaaaa gattggttta tgtggctcaa taatcccttt ttctattttt tctttataac
     2221 gtgtagattt tggatcggcc actaccagca atgttttgtt gagttccata taagcatcgt
     2281 gccccataat tacttttccg cgttcttttt cgcttgctaa tgttgcactg tacagcagag
     2341 tcaactcccg attcggatat tcgaaagcca tatttaatac gtccggtaca gtacggccat
     2401 ctttgtaata atagataccc cccgatgccg tagcggaatg tgggattcct aatccaagga
     2461 tctgattcaa tgcatcgtat tcgtgagtaa aaagatcgcc gcttaatccg gtactataat
     2521 cccaccaaca acgccaacgg aaaaaacgtt cgaggctgaa ctcgtgccat ggtgccggtc
     2581 cgataaattg cttccagtca atagtattct catttgcatc aggatcaatt tcgtaaaccc
     2641 atgctccatt tgggtcattg cggttagtgc atacttctat gagcgatatt tttcctataa
     2701 caccaatgtt aatcagcgat ttagcaatat gataattctc tgtttgtctt ccctgatgtc
     2761 cgagttggaa aatgatttct gactctttca cagcttctcg aattgcaaag gtctcctcaa
     2821 cagtacacga cagtggtttt tcgcaataga tatgtttgtt atttttaact gcctccagag
     2881 caacaggaat atgcaaatgg tcgggcgttg caatgattac tgcatcaaca tcgggtgaag
     2941 ctaccaactc ctgataagtc aaaaatcgtt ttggcgcatt acccatctta ccaccagttc
     3001 cgttcctatg aatatttgca gcagtctcaa cgccccagtt agcattttta tcaaatacgt
     3061 cgcaaatagc tgtaatttca atatttaaat cttcctgagc gtaaaagtca ttaaggtatt
     3121 gagcccactc gttacttttt tttgcagatt cgatgtattg atcaagacgt gaaggatgca
     3181 ggaaccccaa tgctttcatc agatatttac cacgactgcc tgtgcctaca attcctaatc
     3241 tgattgtttc gccttcgaca accggtgata tccctttggg taaaatcgag ctgatgtcgt
     3301 caagcagcgg cgctaacttt ttattacgat cattgatatg ctgaaacagc ccataaccta
     3361 ttaccccaat aacgggtatt gatgctaagc ctgccaggat ttcccgacga ctgtactgtt
     3421 tagagcctga tattgtatta tccgaatcca tttttatttt cttcggtttt tattaaaaaa
     3481 tctttttatt ccaatgatat gcgctgttgg aaagcagcaa attacagcta atgaagcgat
     3541 ttctacaatg gttttattta ttataaaata actgccatcg cttgggaaca tataatcaat
     3601 gccgggaaaa ggaggatgcg acaaataata taggctgagc aaaagcatcc ccattatagt
     3661 tgcaaggtgt tcgaaacaac cgagaatgag aaaaaggcct atgagtgtaa gtacccaggc
     3721 attaatagtg tcattgaatg tcaacaacgt ttggtttttg gcaatccata caaaaaagga
     3781 actggcaaac cctcctgaat caagcagata agcttttgac gtccatgatg gtgtaaaaat
     3841 ttttgttaat ccttcgtata aaaaatgcca gcccacaata aacctcaatg ctacaagtga
     3901 aatgagctgg ctttttgaat aattcattgt tgatcagttt tgtttttaat gtttttacta
     3961 agttttacat tatcagtaca aaacaagctt tgttacttca taatttccat cggtagtaat
     4021 aatcataata taaagccctt taggcagcgt actaagatca agttgtgcag aacagccatt
     4081 ccagctatag ttacccatca aggctccgct ggggtttaat atttttataa atgatcctgt
     4141 tgcagggttg tttccggggc caataatatt aatcagtccg ctgctaggat ttggctgtac
     4201 taaaattttt gccataacct ctttatttac acctacgaaa tattcattag agaaactaat
     4261 aatattacaa gctacttcaa gcaccggatt atagccatca ttgcaatcgg cacaagtaac
     4321 tccggtaaca caatccgggt gatttggatg acagtaatca acatattgtt cgtagaaaac
     4381 atacttgaga gtaacgttac tacctgatat gaacgaactc atatcgtagt cgaaccacgg
     4441 agctatcgag ccggggcacc accctgcccg attatgatac catgttccat tttgcggttg
     4501 gcagccatca gggttaggat tacatgttga ccagttatgc tgtgtaaagg tctgttcgcc
     4561 gtttacccaa atatgatgtg ttgcgtcgta aaattcggca gcgttgccgg tatttaaatt
     4621 tccccagcca tggccggtag ataccagctt gagcttggca gctactgtat taggggaaaa
     4681 ttgaaacatt tgatcaggca ctggttgaag attggcataa tcaccaaaag gataaggcgc
     4741 tttccatatc tggttaacag tactataaag atattgtgga gctcctgcac gataattcaa
     4801 agtaagttta taaagatatc cattatccag cgttgcacaa ttaaaacgaa tgctgacttt
     4861 gccacccaac agcgaaatat aatctgtcag gtcaatcgaa tgagaacatg gtactccata
     4921 tggagtaatg taacggataa tctcaaacca ctctccatca aggcttttag catcaatact
     4981 tgccaattta tcccattcgc cacatcctcc cgggggacaa ctaacctgaa gcgttccaat
     5041 tacctgatca aaagctccga gaaaagaggg caaagtagcc tcaacggttt gtgtatctgt
     5101 tgatggatta agccatacgc cttcggcagc cacaatttcc aagttggtag cgttggaggc
     5161 tatattaata tctacgttct ttgttgcaat agctccataa ttattggacg aaatgatact
     5221 aattgtgtaa ttgccataag ccggaggtgt ccaccaagca gtataatatc cattatccag
     5281 cgggtcatta gcaggtatat tttgtccgtc aactataaaa ttaacgctct ctacactaaa
     5341 aagctcagga tagccaatgg ttgataacgc tgtaagtctt aatttcgaaa gagttgacat
     5401 atagaccgtc ccctccaacg gatgcctcgg atcaatatca ggaacataaa cctcagggtc
     5461 caaaaccata aaagtattga taatcggtac tgcgggatca aattgcgagt ttcctggctg
     5521 ggtagcttta atgctcactt cgccggcttg gccggtaagg gttacaatat taccttcgat
     5581 agtggcaggg ccggaaatta cttcgaaaaa gacaggtagc cctgatgtag cagtagcttc
     5641 aatttcgaaa ggatcatcag tggtaagatg gttagggatt tgtggaaaag aaatggcttg
     5701 ataaccggga tttaaggttc cattgaaatt ggatgtttct ccagtcaatg caaaattcat
     5761 caacaatgca tcgcgctccc catttccaat ctcgcagatg agatgcgtga ttgcagtatt
     5821 gtttccgccc ggctcaccct gattaaattt atagtacaat tccagatctt gttctgttcc
     5881 agtgagttcg ttatcaataa tgtcttgtat ttcggattgc gagagagcct ttttccaaac
     5941 cgaaacctcg tcaatacgac cgccaaaaac aaaattaaaa ccggcaagcg tactttttcc
     6001 aatagcaaaa ggtacattcg ctccctgaaa gattcctgat gcagcagtac ttcctttaag
     6061 agcgccatta acataaagtt tgatagtatt aacatcgaac acccatgcaa tatgttgcca
     6121 aacttggggg attactgtat tggccggcgc tacatattca tgcaatccgg ttgtagtttt
     6181 aagacgacat tcaagtactc cattattaag ctggataatg taaaactcag cgctacccga
     6241 accgatccta aatccaaagt aaccttgacc gtatgaaagc tcatcgcagt aaaaccatcc
     6301 agtcattgtt aactgagtgg tgccggaaaa atactgtgaa gcgtcattca gtattacaaa
     6361 atcatcaatc ttatcaaaat gcaaatattg attagtttga gagtttgctg ataatgaaat
     6421 cagaacagaa cttgaaatca tcatcataat cagaaagtaa taaaactttt tcatggtatt
     6481 tttttttggt taataattat tattttttat ttgttgtaat caggttaaag ttaaggcatc
     6541 caccaaattt ttatatccgg cgtgtctcct ccttgtcttg ctactgcttc actatagttc
     6601 tttaaattat tttcagcttc tgacggagga tatggcaatc taaaatgatt tattggatcg
     6661 ccttcgtgcg gagtaagtaa agtgcgtctg gccagcgaat aagcttctat cggctgtctg
     6721 aatgcatcga gccatctctg tgtatagata aattgtagtt tctcgtcatc ggaagtagca
     6781 ttccagaaac caaagtgcat ctgtactgac gacgaactga gagaggatgg tatctgaatc
     6841 atttctggaa aactcatccc cgacaaaggc agttttaatc cttcagctac cttcaaccac
     6901 cattcaacgc tagcattaat tccgttaaga tattctattt cagccttcat catatccatt
     6961 gctactccaa tacctctcaa atatgcctcg gcttttatat aatgaatttc ggctcctgta
     7021 attatcggga tcggcatgtt atcttcatct ctcacaataa aataattgaa cggtgcatag
     7081 atacaattct ctcctttaat atcaaaattt ccagcctgat cacgatggcc accataagga
     7141 ataccacctg atgaaggagt tgcaacactg ggtaattgag gaaaaaaagt ccatttgtta
     7201 aagttatcgg tttcgaaaaa aatgtatgcc cgcggatcaa agattccact cccgtcggta
     7261 ctgtcattta aagagagctg atgccaaata ttgcttccta accgaagtcc attgtgttct
     7321 cggaacgacc agttgaggct ttcattttta aaacccataa ctgccggcca gattgccgca
     7381 gcttctaatt taggagttat gaaatcgtaa ccgtaaaaaa caggtttgtt gttttctatc
     7441 acatcgctga tcacaagtcc ggcaacttgc ggctcctttt cgctcatccg caaggcatag
     7501 cgcaaacgta atgaattagc gagctttttc caatttagca tatcgccgtt aaaaagttta
     7561 tcaaaagctt taaaagtaac aaaaggttcg gccattgttg ctgtttcggt tatgttatta
     7621 tcggcccatt caagatcgtc taataacttc aaatagatgt cgcgttgggt gtcgtatttg
     7681 ggacgtatta aagcaacatt gtggaaacca taaccagcct cagaaaaagg aatgtcgcca
     7741 aataggtcag ctactttaaa agttttaagc gctaaggtaa ttttcaacat agctttcatg
     7801 tttaatacct ctggcgttgt ttcgtaggtt tcaaaccgtt tttctaattc tctgacttca
     7861 ggcaatgtta agtaataatt tctccagata tcttcggtgc cgattgtaaa atttccccat
     7921 gcgtctttgg ttagcgcagc taactggctc tgtttatata aaatttcatt ccatatgtaa
     7981 aactgttcat cccagccagg ttgcaaagag ctaatcacac tattaaaaag tgagccatcg
     8041 ctggcagttg tgaaaccgtg tgggtttgta ttaatatctt caaaatcttt cgtacatgcc
     8101 gtaaaaataa aaagcagaaa aacaaaaaca gggaacagca gatttttaat gataaaacaa
     8161 ttgactttca ttttcttgga ataatttttt ctttcgttat atggttcagg ggataagcaa
     8221 ttttttactt ttttcttact atatttaatc atttttgacg tcatctgttg cagcttttaa
     8281 attagttaaa atgaagcagt taatccaaaa acaaatgatc ttgtgccggg aaaagatgcc
     8341 cactcgaaac cttgggcgtt tccagatccc ataattcctt ccggattaat tttgtcgggt
     8401 aatgttgtat aaatataaaa gagatctctt gctgtaaatg agaccgttag attttggaaa
     8461 gcttttaatt tctcaagcca tttttgcgga atggtatatg ctaaagaaag ctctctcatc
     8521 tttacccatg tgttttctaa gatgccgggt gtggaaacaa actttcccca tccaccggca
     8581 tttggaagat acttataata ataatgaaca accgttgtgt tgggagtccc gtcttcgtgc
     8641 actccatcaa ggataatccc aatattactg gtattaccat caggatcggt gtatggcaat
     8701 ccatcgcctt cacgttcctt aagggtttca ggactttgac ctgtttgcag ccctatcacg
     8761 taagagccgc aatatatatc ccctccccat tttgtgtcaa tcaacattgt gaatttaaat
     8821 cctttgtaag cagccatagt attccatcca ccgataaagt gaggcgctgc attaccgatt
     8881 ggaacacgag tatcggtaat aagatatttg gtgccctctt cattaacaac acgatttccg
     8941 ttttcatcaa aaatataatc gtatccgctg atagtcccgt agctatctcc ttcgtggagt
     9001 atcattgccg gtccatttaa cccccagata tcggcaagca tatagctatc ggcagagcct
     9061 ccaaggctta caactttatt acggtttcgg gcaatgttga tccccgaccg aacaagccaa
     9121 tccttagttc taaccggcac agtattgatg attaactcaa tacctttatt tgtcaacact
     9181 ccttcgttgg tcattatggt ggtagctcct gaagaaaagg gtaatggtag attcggaata
     9241 atttgatcaa aagaatagag ataataatat gtaaagtcca tatcaatcct attatcaaaa
     9301 aagccaagat tcaaaccgcc ttcataggag tttacccgtt gaggtttaag attcaatgga
     9361 ggtatggttg ccgggaaatt ggaggcttgc tgcccgccaa aaagtgtggt attgtaatga
     9421 aaagtcatca gataaggatc ggtgtcggta gctgtttgtg caatacctcc tctgattttc
     9481 ataaaattga gccaattgat gtgtttttgg aaatcaataa cttctgaagc aataaaactt
     9541 aaggagactg acgggtagaa ataggaatta ttcccttttg gcaaggttga tgaccagtcg
     9601 ttccttccgg taagctcaag aaaaagataa ctgtcgtaag cgaggttcaa aaacgaataa
     9661 acagaattaa ttcgtttgcg gataatttgt tctgatgggg ccattgaccc tggagtatta
     9721 ccaaactgtt ctactatggt gttgttgttc gcatcagtag agtagatatt gtcggtgtag
     9781 ttgaaaaagg tgtacatgtt aggataatac catgtgccgg aacgtcctcc tatataataa
     9841 ttctctgtat tataccggct tgttcccatc gaaaaagtta aatctaattt tgaatcaagg
     9901 atttcatctt ttttggcagt cattgtaaag tcatagttat cccccacatt tcttgccaga
     9961 ttattgctgt aatatccatt ttgcagtcca agagcatcta ttggcttatt ccgggtttca
    10021 tattgttcta ttgtaaagtc acgccctatt cttcctaata cctgaagcca ggatgtcata
    10081 tcatatatca atgtgagtgc ggcagtgtac ttatcgcgat cgagtgttgt gttgttatta
    10141 taataatccc accagatagt tttgctaaga tacagaaatg gatatccatc ttgcgggttt
    10201 tgtgttccat cggggttggc atagttcatt ttatcaacag cctgatagct tcttggccat
    10261 gaatacaaaa gtcctttact gaaagagttc cagtcttcgc cgagcatagg gctgttcagg
    10321 cgatggtaat tgataaacga aagggttaaa tcagttttta gtttactgct gatgtctaag
    10381 gttgcaccta agttaagagt cgtgcggtta aattcactgt tttcaattat tggtttatta
    10441 tcttgccttg ttatggaaac acgcatagtc cctttatcgc caccacctgt tgccgaaata
    10501 ttgtgtgtgg tagtgtaacc atcggagaaa gggattttca aattgtcggg ctggggtgag
    10561 tatggcctca tttttccatc ccaccatttt atcatttgcc cctccatttt aggtccccac
    10621 gagactgaag aaccataata accaaactct tcggctgttg tcgaggtttt tccctcttga
    10681 tttattacca attgggcatt gtcataaatc ccaggataaa gaagcgtgtc gcccgatgtt
    10741 ggaaacactg gtggggtaag agagataggc ccaccatgtc cataaatgtt ctgtacttca
    10801 cggtaccgat atggatgagt gatacgataa tccatattat aggttacacc tattcctttt
    10861 tgtttttgtc cgcgtttggt agtaatgaaa agtactccat ttgctcctct tgatccataa
    10921 agtgctgatg cagcgcctcc tttcattaat gtcatacttt ctatgtcgaa agcgttaatg
    10981 tcgtttattg cgcttcccca gtccacaccg cgtccaatat tagagaggcc cggaacattt
    11041 tccataggta cgttatcaac cacaataagc ggttggttat cagcgccaat attattatta
    11101 cccctgatta caatccttgt tgaaccgcct tcaataccat ctggctggct aatttgaacg
    11161 ccggccgaac gccctgagat tgcattgaga atgtttggcg cccttgattc tataagaaca
    11221 tcttcatcaa tcttttcagt tgaatagcca atttctcttt tttgcctgct aacacctaat
    11281 gcagtaacaa ctacctcctc aacttcggca tactttgtaa gcatcctaat ctctataaat
    11341 tgttgttctc caacaaatat ttctaccgtt tcgaaaccga tataactaaa aaccaaaata
    11401 gttgaagcct ctacagatgg taatgtaaaa cgaccattga catcggttac agtaccggtt
    11461 agcgtgcctt tgattaaaac agttacccca atcaatggaa tatcgtcctg atcggaatag
    11521 actttacccg aaacagattt ttgttgcgca aacccaaaaa cattgatcaa cagaaaagta
    11581 aaaagggtaa ataacttaag tctcataaat atatagttga gtgagtgaat actttttaag
    11641 ttatcaaaag taaaagaaat tttaatagtc tttcatttta caaaaaaaaa tgacctcctt
    11701 tttcagggtt tgaatctgcc ggatagttat aataagcttg agtcttgatg agttgtgccg
    11761 agcaaaaagt cttttatgtt gtcaattata tcatggaaaa gctacctcat cattgtaatt
    11821 atgatcacga tggggatttg tcttctccat tcttctattc caaagcatta cattgacata
    11881 atctacctgg caattggaat agcattggtt ctgttgatcg ttcgttgttt cagaaattta
    11941 ttgaaacaaa gttaattact cataaatcaa tcccgaagtt taagctaata agtttcttaa
    12001 aattgtgata ggaaagcgac taagttatcg ctacgtttca gattaagttt tttccttaat
    12061 cgtgttcgtg ccatctctac actccgaatg ttttgatgag ttattctcga aatctctcta
    12121 gaagacatat tcatgcggag caaggcggta atcttttttt catttttggt gagcttcggg
    12181 tgttttttat aaagattatc gaaaaatgat ttgtttattt tctcgaaagt taattctatc
    12241 tccggccaaa gaacttctat attatctttt tttgcctcct caaggatgtt ttctaattca
    12301 ttaattatag aagctggtgg gttttgcaca acattatcca aatttctgag cacttttttc
    12361 agttgcccat ccttttcact gatttgtgca agttttgaga tcatctctct attcttacta
    12421 tcaatttcat ttttgagtct ctctatttct ttttcgtaaa attcattttt ctttttttca
    12481 gcaatctttt cgttggtaat atctctaata aaagcgccaa taagtgtggg agtcccaatt
    12541 tcatttttaa taatccaccc ctttaccata gcaggtaaaa tggagccgtc ttttctcagg
    12601 tagcttcttt caaccggctc tattgtatcg ccaaaaagta tagaaagttg aagtgttgag
    12661 gcttcaacct ctcttttatt ttttggcgta atatcaaaca agctcaattt agtcaattcc
    12721 tcttctgtgt aatgaagcat tttacataag gtggcattgg ccataataat tcttccgtca
    12781 atagagctta taatagcgcc ttctggtgag gtattaaata acaacctgaa attcttctca
    12841 ctttcaagta acgcattttc taaattgaca cggtcggtaa cctcagtgga aaatccgata
    12901 atacccactg tttcaccatc ataagtttta tacggtatct tgtcggtaat aaatttacgg
    12961 tttggatcat taaaaagagg tttaatcaat tttcgaagcg ggatgtcgct tttaataact
    13021 tcgagatcgt ctttccagaa atcaattgca agctcttgat ctgatgttag ctctgagagg
    13081 cttcttcctt gtacaaattc gttcgtcagc ttagttgtct cttcaaaagc acgattaaca
    13141 cttacaatcc tgttttccaa gtcctttaca taaactaatg cgggaatgga atcaagaata
    13201 gtttgtgtgt attgttgttc tttataaagc tcttttatta ttttttgttg atcagtaaca
    13261 tcctgaatat agtaaacaaa aacctgatca tcaataaact ttataggctt acaagttaca
    13321 ttaacgtcaa tactaccgcc cgtaagcttt tttatggaat cgttaaatga gtacgatcct
    13381 ttttcttttg aagtcttcat aatttctgcc cacttagctt tactgtagga gaaaatatgt
    13441 tttacctctt tttgttttaa ttcttgaaaa aaagtagctt cagctatttt gttggcatat
    13501 aaaatttcgt ccttttcgtc agctactaat aatgcaatcg gtgcaaaatc taaaagttga
    13561 agataatcag caagtatcac gtaaatatat gatttttaaa gttttataat aatttttttc
    13621 tatttacttc caattaatct aaggatatca tttccattgg caaaaattaa aagcccaaaa
    13681 aggaatagca tcccgacaat ttgcgcatat tctaaaaatt tgtcgcttgg tttacggcgt
    13741 actataattt cgtaaagcaa aaacataacg tgccccccat caagagctgg tatcggcaaa
    13801 agatttaaaa tagcaagcat gatggaaaga aaagcggtaa gactccaaaa agcccgccag
    13861 ttccaggttg atggaaatat tgatccgata gtgataaaac ctccaacgga ctcgtaagct
    13921 tttacctctt tggagaacaa aagacgcaat tgtttaagat agttggccac ctcatcaaaa
    13981 gctttggccg ttccggcagg gatagcctga ataagtgaat agctcttctc cttcaagttg
    14041 aaatattctg atggattttg aggatatata ccgacaattc cttcgggcga tacggccact
    14101 gtatttgata aagtatcacc attacgaata accgaaatgt tgacattttg ccccttaaaa
    14161 tgctgaattt catcccgaaa ctcatcaaag aaattcactt gtgtatcatt aattgcaata
    14221 attttatcgt ttatctgaag accggcctct ttcgcagagg agttttttgt aaaatcgcct
    14281 actacaaacg gaaaacgtat tccaatcaaa tctggactcc ggtgatggat caatttattg
    14341 agaaagcctg aaggaatttt aatatctttc atttgaccat cacgtgaaac ctgaatcgtt
    14401 ttggcttcat ctataataat ggtgggtagt attttatgaa aatcctcaac ctctttatta
    14461 tcaatcgaaa gaatatagtc gccgttacgc aaccccattt cataagcaag cgaatctaca
    14521 gtaatgccat atttaacttc agcagtaggt aggtattgtt caccccaact gtaaagcatc
    14581 acaatgtaaa taactattgc caatagaata tttactaaaa cgccgccaag cataattata
    14641 agtcgttgcc atgcaggttt tgacctgaac tcataaggct taggaggctg tttcatttgt
    14701 tccttatcca ttgactcatc aatcattcct gagattttta catatccacc caatggcaac
    14761 caaccgatcc catactccgt atctttgatt ttgaacttga aaagctcaaa ccaaggattg
    14821 aaaaataggt aaaacttctc gacacgcgtt ttgaaaatct tggcagcaat aaaatgacca
    14881 agctcatgaa aaataacgag tatagagagg ctaagtaaaa acaatgatat tttagtaact
    14941 atttccattc tatttaataa gcaggtttta aaaattcctt tacggtcgaa gttataagtt
    15001 tttttcagta tttattcttc ccttttttta gttgttttcg gatcaacttt ttcagtcgtt
    15061 aatcgcattt gattttcagc ctgttttcgg ttcttttcgt tttcttatca ttaagtggtt
    15121 gtttttaaac gtgttagtct tgttcaaatt gctcataaaa atactattta cgaatgatct
    15181 ggttagctta tctgtgtgaa taaggtcttc taacccaggt tgtctaatat aatctatttt
    15241 ggctatgctt tgttcaatga tatctgaaat ttgcaagaat gaaatcttat tgtttaaaaa
    15301 caattcaaca gaggcttcat ttgctgcatt caaaatgcaa ggcatgtttc ctccttctaa
    15361 caaagcgtgg taggctaaat caagatttct aaagcttttt ttgtcgggtt taaaaaaagt
    15421 aaggttggag tttttatcaa aatcgaggcg cgggaatggg gaactaatcc tatttggata
    15481 cgaaagggca aaatgaatag ggtatctcat atcagtaggc ccaaactgag ctttaatgtt
    15541 gccatccacg aattgcacca tcgaatgtac caccgattca ggatgtatta acacatcaat
    15601 ttgatgtggt tcaagatcaa acaaccagct tgcctctatc acttcaagcc ctttgttcat
    15661 aagggaggcg gagtcaattg tgattttttt acccattttc caccgtggat gctgaagcgc
    15721 ttgcgataca gtaacatttt taagaaactc aatggatttg ccaaaaaaag ggccaccgga
    15781 agcagtaaga tatatcttct cgatactatt gatctgctca ccaacaagac attgaaatat
    15841 cgccgaatgc tcggaatcaa ttggtaaaag actgacacct ttttcttttg acagtttgga
    15901 aattatttca ccggcaacta ccaacgactc cttgttagcc agcgcaaggt cttttcctgc
    15961 tttgatggcg ttaattgccg gttttaatcc ggcaaagcca actaaagcaa gaagaacagt
    16021 atctatctgc tctaactcaa cgatgtcatt taaagcatca tttccagtgt aaacagatat
    16081 gtcgaatggt tgcaaggccg acttaaccag gttataagaa tcccgacaac caattacaac
    16141 ggcatgaggc ctaaattcaa tagcttgctc aatcagtttt tgggcattgt tgtgggctgt
    16201 caatgcaata actgtaaatt gttgcggatt ggctttgaca acctctaagg cttgtctgcc
    16261 aatagaacca gttgaaccaa gtaaagctat atttttcaaa tattggttgg taaagttatg
    16321 taacaaaaat acattatttt tttaaaatgt aataaactct tccgggtcaa caggttttcc
    16381 gtcataccat aactcaaaat gcaggtgaat accggtttgt agctcgcccg actggcctat
    16441 aaaagatatt gactcaccag ctttaacttt tgaaccttgc tgttttaaca aacttgaatt
    16501 gtgtttataa accgacacta actgatgatc gtgctgaata caaatgatat aacctgtttc
    16561 gagcgtccaa tccgaaaaaa ttacagtacc atcaagcact gacttaatag gaacatttgg
    16621 ggcagctacg atatcaactc caaaatgaga ctgtatggga ttgtattttc ttgttatgat
    16681 tccattaacc ggcgagaaaa aatagatatt gccgatgctc aatgtcggat aggtgaaaat
    16741 attggtagga taaagcgtga actcagtttg actctcatat tcttcgcgaa gcgccgaatc
    16801 ggctgccgaa tgccttaaag taatcgtgtc gtaatatggt gttgaagtgt aagacattgt
    16861 aggttgctcg tacatatcat catcagagag tatcctttta aggttatcca tgtatgtctg
    16921 acgatgtttt aattcaactt ccaacgagtc ggcctttcgc tggaggttgt aaatttgttt
    16981 gggcaaagat agatcggtat agccagggat atattctcgg agcgaggtaa aggcaacaag
    17041 gtaaatagtt aaaacaataa gtgaaacgat agttaggcca actactacaa atactccaag
    17101 ccgagtgatg acaagggaaa ttttctcttc ataggtttga acatccatca aaactaatct
    17161 atacttacga agtaacttag gtgaatagcc taaagtctgt tttttcctcc ttttcatcaa
    17221 ttatcaataa tttttttgcc gagatttaaa atttaataaa taggcttttt tacaaaagaa
    17281 aacgtccttt tatttgattt tgttacaatg tatgattaaa agatcataat tttacgatct
    17341 cacaaacgta ataaataata tttaaacgtt tttcaagtta aaaagcaaga ttgactattt
    17401 agtttaataa tcatacaaca ttgatttgac aaagattaaa aatacagttt ttaagggaaa
    17461 cctatcacat cgctattttt cactgttttc ttttatcgca atagttggta gtatattatt
    17521 agttatttca tgttcaacaa agaaaaattc ctttactagc cgtacttata ataatctcgt
    17581 ttctcattac aatacatatt ggaatggcag ggagagcttt aatgaaggtg ttaggaatct
    17641 ggagagagat gcaaaagata attataccac catattgcct gtatttaaat ttggaaatga
    17701 acaggaggcg caagctgttg catttaacat ggatcgggct attgaaaaat cttcaaaagt
    17761 catccttcgc cactcgatgt attttcaacg gaaagaatac gtcagacggg tgaaagacag
    17821 ttatatgctt attggaaaat cctatctgta caaacgcgaa tatccaaatg caagacgtac
    17881 tttcgaatgg atatccggtt tttataaaga tgaagcagta aaatatgatg ctgacttatg
    17941 gttagcaatg acatataacc aaaccggcga gttcgaaaaa tcatccatgg tgctcgacaa
    18001 gttaaataaa gatatcagaa caggtattgc tccagcaaaa actgacagac ttttccaaat
    18061 gatctatgct gattattaca ttttacaaaa cgactatcag ccgtcaataa aataccttaa
    18121 aagagctata gagttaaaca gtcgtaaaaa agataagaac agacttaggt tcattctggc
    18181 acaggttcat caggaaattg gagacatgaa agaggctgca aggctctatc aattattgat
    18241 tagaaaaaat ccgccctttg aaatgtcggt taatgctaaa attaatctgg caaaaaccta
    18301 cgataccaaa agaagtaata ggaatatgat tgttaagaca ctggaaaaac tattgaaaga
    18361 tcataaaaac agcgaatacc gagatcagat ctattacgca ctggcggata tgtatgctaa
    18421 agataatcaa acggaaaagg tgatagaata ccttgcatta tctgtagcaa caagtgtttc
    18481 caacgattac caacagacac tatcatccct gaagcttgcc gatatttatt ttgaacagcc
    18541 agattatata tttgctcagg cctattatga taccgcaatg caagttatgc caaataactt
    18601 cccaaattat aaaagtataa aggaaaaaac agaagtgcta accgaactta tcaccaatct
    18661 gagaaatatt agcatacagg atagccttca aaatatggct aaaatgccgg aagctgaaag
    18721 aaataaaatc attgacgaaa aaattagaaa aatagttgaa gaggaaaaaa gattagcaga
    18781 agagcagcgc gaagctcaac gcactgtttc gatgcttgaa caaacaaaca gaatggagaa
    18841 caggaaccta cagcaatccg gcggatggta cttttacaat ccttcagcaa tgagctttgg
    18901 ctataccgag tttattaata aatggggacg acgtaaactt gaagatttat ggcggttgag
    18961 caacaaacaa gcatctatag aattcgacga attagctgaa ggcgaagaag gtgaaaacgg
    19021 cgcagagaac gacagcaccc taaaattaga atcagatcct aaaaaaagag aattttatct
    19081 tcaaaatctg ccattaacag aggaagccat ggaagcttcg caaaaaatta tagagaatgc
    19141 actcttcgaa gccggatata tttataaaga tggcctgcaa gacaacgaac attcaataca
    19201 agcatttgaa gaattaatag aaaaatttcc tgaacacgaa aaccgtctga tggtatttta
    19261 tcagctttac aaaaattggg aagaagcaga tgataggaat aaggcagatt attacaaaaa
    19321 tttgattata agagactttc ccgaaaccga ttatgccaaa attttacttg atcccaatta
    19381 taatctcgtt gtacaagctc ggttagatgc tgcaagtaat ttatattacg atacttactc
    19441 ggcctatatg aacaatcagt tctatttggt aataaacaat tcgaatcttg cacaagaaca
    19501 atatcaggac agcgaactga ttgccaagtt tgaatttctg aaagggcttt cttttggaaa
    19561 actacaagat gtggatacaa tgcgtacaac acttgaagat cttattgcca attatcctga
    19621 aagcagtatc aaacctttgg ctcaagacat tcttagcagg attagccaag acgaaacagg
    19681 tcagcttgtg cttcatgaaa catctgtaaa tgttgaagaa aacgaaataa caggtgagaa
    19741 tcctgaagaa gagtttacat ctatctattc ggcaaataat tcttctgtgc atttttatgg
    19801 aatgatcgtg gatgcaagcc aaacgaatat aaatgcactg aaagtcagat tgtcagactt
    19861 taatgcgaat aatttcagat tatcccaact aaaaatcaac agcatagtat tcgacaatga
    19921 tcttcagtta gttactgtcg gtaattttga aaatgccgaa aaagctatgg tttattacaa
    19981 ctctgttctg aataatgact acataacatc tccgcttgag ggaacagata catcattttt
    20041 tgttataacc actgataatt atcccctatt ttatcgggaa aaagagatac agggctatat
    20101 gaaatttttt gaacgtacat atcttgaatg atattttttt ggcgtttttt tgtaaaaacg
    20161 tgaaaaagat ttaatttcgc aaatgattaa tcactaaaaa ttttggaacc atgtctaaac
    20221 attctacaaa cgatatatcg ccatcaatca acttcttggc aaatggtaca agtatggctg
    20281 gtgagataaa atctaacggc gatttcagaa tagatggaac gcttgaaggt tcaatatcct
    20341 caaaaggaaa aattgtagtt ggctcatcag gaaaagttaa aggtgaaatc gtttgccaaa
    20401 atgctgatat ctcgggcgac ataaaagcga aaatagtggt acacgagctg ttgaccttaa
    20461 aatctacggc agttttaaac ggagacattt ttaccaaaaa acttgcaatt gagccaggtg
    20521 catattttac aggaacatgt aacatgcatg acattcctgg aaatgtgaaa gacgaaatcc
    20581 aaaagaaaac tgaaataccc gcaccaaaag aacaaactaa agttaacgtt tgacaattat
    20641 tctcaaaacc aatattgaat tttcatctcg ggcaattaaa atctgaaaaa ggtgaatgat
    20701 aaccgcaagc agtttttgaa attcttttgg ataaatacga ttgtaatgct gctcatgttt
    20761 gcgttgcatc ttttatcaat agtgttgttt ccatcagggc caatatcccg aatgacgccc
    20821 tattccattt tgttttttag catttttaat cttctgtttt tctatatcaa aaggtggctg
    20881 tatcaatcca aagattcaaa atttattaat ctgtttttga tttttaattc ggtgaagatg
    20941 gtgttgttta tagccattat tgccgtctat gcttatttct ttcacgacga cgctatcaat
    21001 ttcgccattg gcttttttat ttgttatgca gtgtttacaa ctgttttagt gagaagcttc
    21061 aatcgtcttc aaaaagtcag ataatcattc gtagccttat gctctgagca aacaggataa
    21121 tgatgtatgt gaaatgcttt tatttatcct tttgattctt acacacgttt tgtctgcaat
    21181 tttctgaaca ttgataaatg cgtaatcagg ggttatgcgt tcagatagtt tcttttggtg
    21241 ttatacaaaa gcatgtattc cctttttgca gtatcgctca gaaatgaaca agggatcagg
    21301 acctcaacca ataattgttt tttcaaaaac ggagtcaata gtttctctat ccggctcgtt
    21361 gcaacgccaa ttctttttgc caattcaata aaatcagctt aactaacatg attgcttttt
    21421 ttaaaaacat cactcttgaa atcatcaaca aacggccctt tatacaaagc aaagtcggtg
    21481 tcgtcaacat gcagtctcgt attcgccaaa taataagcgg gatttaacag gtaatcgccc
    21541 tttgaagatt caagtaatga aaaaatttta aggtgtgcat cgccatttga aaagagaaag
    21601 ttgaacaaaa caagtaaaaa gtatttctct atttccaccc tccatgcagt aacaaatttt
    21661 tgcagtaata atcctatttc ctcgtagcta tattcgtatt tgaaattagc tccggtattt
    21721 ttttgtttta cctgccagct ttgcaaaatc cttttttccc catttgccac cttcattttt
    21781 cacatcaaac cgtttggtta agtaggcagg tgagccatcc ttaaaaaata ctagtgcatt
    21841 ttcagcggtt ttgataccat aaacttaccg agcaatttgc attgttaagt gttcatttgc
    21901 cggaacctga ttagcttttt ttaagtctat tggtataggc ttcaatatgt aagttccttg
    21961 ttagccctct ttagtaaggc gcaaaacatt tttctccaac atgaagctta atttttcctg
    22021 aacgcctggt atggatatat ggtttctgtt ttctatgaat tgcattacta cttcttcact
    22081 cagttgcggc aaattataag acaatacatg gtttactttt ttacctttaa acaacaggcg
    22141 aagtcaatta ggactgtatg ttgtaaatcc tttagccaaa gttccggggc aaacctttat
    22201 ttcattcaaa ttcatttgct caatggttta attgttattg cccctatagt atcatacatc
    22261 gctgttgctc caagcaaacc aaagttatcc tccaagtcaa ttcgcaattg tttgcattgt
    22321 ggttttttgt ttactccttc actcagcata ttaaagaaga aaggaaccag gaatttactc
    22381 ttgtattcct gttgtgattt cagcagtgtt aagctgcttg ccggcatatc actgtttgta
    22441 aaatatgaat cgtcataacg gaacacgtaa tatttgcaat cctctccagt aaggatacca
    22501 gccagaatgc catttcgata tacttgcacc cttctcataa cattaatatt ttattgtcca
    22561 ttgacaattt ttttaacttc tattttcagt tccagcccca gcgcttcagc taactttttc
    22621 atcacatcaa gcgatggatc cccttgtccc agttccaatt tacatagcgt attagtactc
    22681 actttagcta attcggctaa atgtggttgg gctattccca actcttttct acgattacgt
    22741 gtggtcttgc caaggtggtt ggctaacatt ttaatttgat tttgttcttg ctttccataa
    22801 aaagaaagag tcaatcggca gttatcatat tataatagaa ttattgccat gaagaatatc
    22861 aatattagtg tagctaattt acataactat acctgacgat ggcaaatgca gtgttttttt
    22921 aatagatctt actttaataa gttggcgggt ttacgtcgtc ttcatcaaag tacaggttga
    22981 cgtggtggat taactttgag gctgtaagat cgctaagtaa ccgggagttc tttttctctt
    23041 ctttgagcga aacatgatag aaggcttcta atacttgatc tgatccaacg tttttcagat
    23101 ttactaattt tattttccga caatgttttt tcaaaatacg ttctatttga gtctctgttt
    23161 ccgggtcgcc tagatatgta atttgcaaaa ggtaatttct ctttctggcc tttccgaaat
    23221 tagtgagcgt gagtataatc acagctaagt tt
//
LOCUS       DAOOAI010000025        22540 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_3030_length_22540_cov_5.216856, whole genome shotgun sequence.
ACCESSION   DAOOAI010000025 DAOOAI010000000
VERSION     DAOOAI010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22540)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22540)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22540
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3030_length_22540_cov_5.216856"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(182..475)
                     /locus_tag="PK345_03085"
     CDS             complement(182..475)
                     /locus_tag="PK345_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34037.1"
                     /translation="MKAIFIVFNQAHTERVEYILDKLGIRGYSWWSEVKGRGSVDGEP
                     RQGTHTWPEMNSALIAIVEEEKVKPLLERVKKVDEINREVGIRAFVWSINEMY"
     gene            complement(499..3624)
                     /locus_tag="PK345_03090"
     CDS             complement(499..3624)
                     /locus_tag="PK345_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPX34038.1"
                     /translation="MSIYKSAINRPITTFMIFTAVIVVGIYALIKMPVDLYPEIDPPY
                     ISVMTTYPGASALDIETNITKILEDALNTVDNVKEVTSTSTDNLSVITLKFNWGSNLD
                     EATNQMRDVIDRVYDYMPEDVDRPTILKFNTSMMPIVFYAVTANESYPGLDKILEEKI
                     INPLNRIDGVGSVSMIGAPKRIIYIEADPRRLDAYNLTIEQIGGIIASENLNMPSGNV
                     KMGDMDYQLRIQGEFTDSRQLANLVVGNYNGIPIYLRDVAVVRDSLKDLSLDEKINGK
                     TGLRMFVMKQSGANTVKVAREVNKNLNELKKTLPPDIQINQILDTSSFIKGAISNLSQ
                     TLIYAFIFVVLVILVFLGRWRATFIIALTIPVSLIVAFIYLFITDNSINVISLTSLSI
                     AIGMVVDDAIVVLENITRHIERGSSPREAAIYATNEVWLAVIITTLVVVAVFFPLTLV
                     GGMTGMMFNQLGWIVTITVVASTFAAISLTPMMSSRVLKLRKKEVKMKKFSYGRTIEP
                     LLNKLDDFYAKTLTWVLRHKAFVLISLSIVFIASLFSLKFVGTDFMPQTDESRLSISM
                     ELQTGTRVEETMKTARKLENIIQEKFPEVELLNVSTGSDDEGGMFSLFIGSGSNIINV
                     MMRLTHVDERERTVWDIADEMRDIVKAFPEVVTFNISTTGAGMGMGGESNVAIEIFGY
                     DFNTTNRIAEEFRQRLSNMPEAKNIVVSRKNDKPELQVVLDREKMALHGLNSATVSTM
                     IRNRVNGMIASRFKEEGEEYDIRIRLSEKYRNSITLLEELTIMNQQGKKIKLKEIGSI
                     EEYWGPPSIQHKRKERIVTVSAIPNGISLSELAKKIIKLTDEVPVPQDVLVNVGGSYE
                     DQQESFQDLMLLMLMSLILVFIVMASQFESFTMPFIIMFSIPFAFTGVMLALFITGST
                     LNIVAGLGAVLLIGIVVKNGIILVDFTNLMRDRGMRLYDAIVAAGRSRLRPVLMTALT
                     TILGMVPLAFSTGEGSEIWRPMGITVIGGLVFSTIITMIIVPVMYGLMGRSGERNKIM
                     KLRKQFNALDD"
     gene            complement(3651..4688)
                     /locus_tag="PK345_03095"
     CDS             complement(3651..4688)
                     /locus_tag="PK345_03095"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HPX34039.1"
                     /translation="MKHIIYFGLAFTMLAACSGETIKNDGKEKEELAAEPVEVLVIST
                     REITRDVEYTSTVLAWEEVHYAPAAPGRIDNIFVKVGQRVAEGELLVQMDQTQLHQAE
                     VQLQTLKTDFARIDTLNKTGSIPRQQYDQLKAQLDIAQSNVNFLKENTKLTAPFYGIV
                     SGKYFEAGEMYSGAPIPTIGKSAILSLVQIDRLKILLAVSEKYFPMVNKGMPATVISD
                     IYPDKVFEGKVFNIYPTIDPTSRSFNIEISLDNTGELLRPGMFCRVRLDLDREEAIVL
                     PSIAILKLQGSNDRYLFIEENGIAKRIPVTLGKRYNEFVEVISDELKEGDKLIIRGQA
                     RLLDGMRVTVN"
     gene            complement(4705..6006)
                     /locus_tag="PK345_03100"
     CDS             complement(4705..6006)
                     /locus_tag="PK345_03100"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPX34040.1"
                     /translation="MKFKYLVIIVVLLFQASLSYSQESLRLSLMDARAYALEYNKTMK
                     NSNFAIDKAQYALREAISAGLPQINASADYSNALGAKISIRFQEGMPAAEIDIKPQSN
                     LYLNVNQLLFSGNYIVGVQLATLSKEFSNLGLEKTELDVITAVSDAYYAVLITEEMLS
                     ILEKNVENLQDLYRKSEPMVHVGVIEQTDLDQLSVQLTSLLNVRSASERQAELAKNLL
                     RLQLGTSIDTELILLDTLEQLIEDSALENVLLNEFNPEGNIDFRMIQQQETLTRKMVD
                     MNKANALPTLVAFYRYTHKLLKPDFDMSPPNMIGLQLNIPIFSSGQRYSQTQQAKVDL
                     KTVENNKALVEDQLRITERQMRFNFQNALETFENQQKNIEVSRRVYLNLKLKYGQGML
                     SSLDLINADNNYLKAESDYINSMMQVLSTRLQLEKIYGTIY"
     gene            complement(6019..6633)
                     /locus_tag="PK345_03105"
     CDS             complement(6019..6633)
                     /locus_tag="PK345_03105"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HPX34041.1"
                     /translation="METKERILIKAGELFRGLGIKNVTMDSLASDLGISKRTIYECFS
                     HKDDLVISTMLYYIQNENEELSKIVNESKHVVEAMFKIIKHKRELLDSYSPLFKKDIA
                     RYLPRIIKLYYTRADDLKRYSLSYILLERGIKEGVFKEGINIKIVDSFLQDLMVTMHK
                     SQRIVLLKPNEREILENIFIPYFKGLSTPKGIELMDEFFGKLEE"
     gene            6848..8539
                     /locus_tag="PK345_03110"
     CDS             6848..8539
                     /locus_tag="PK345_03110"
                     /EC_number="3.2.1.18"
                     /inference="COORDINATES: protein motif:HMM:NF024488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HPX34042.1"
                     /translation="MKKFFLPVLFFLLIFVQFAFYKAKNFAITEINNTISGIKIIGLQ
                     NDFNPCFLSDSLFNLSNQMMCIDTIIEVTTNTPVGRGNINEGIVAANVITIGTGNPLE
                     LTSLTFSLDGTTDISDIKQININLSFNSTRTSNSLLLKSLKPQKKDITIICNHSLHEG
                     NNFIWVSADLQSTAKEGNYLAGKISSVTIDKIDYPVTPSAEKSNRLIFLEHKLLFSGG
                     DYGSANFRIPALTTANDGSLIVAVDARVDRTGDLPNNIDIMYRRSFDQGNSWSDASVI
                     CDFGMYGASDPALVVDKKSGVILCMFASHIGLFHSTFDNPISFQVCRSEDNGVSWSKP
                     VDHTNSIYAPGWYAAWLASGSAHQLRNGRIVGAIGVRQEADYTISNFMIYSDDVGLTW
                     NYKSAQASAIGDEAKIMELDNGNLIMNIRNKTPNCRKIVVSEDGGDTWGAPFFQHELI
                     DPAVNADFIRYTSVLDGYDKSRLLFSTASHPTTRKNLTLFMSYDEGVTWPVSKVIYEG
                     LSGYSSLTILNDGTIGCAYENGEFEEYQIYFVRLSLDWLTDGKDSYQRPNDILRK"
     gene            complement(8581..9720)
                     /gene="dnaJ"
                     /locus_tag="PK345_03115"
     CDS             complement(8581..9720)
                     /gene="dnaJ"
                     /locus_tag="PK345_03115"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HPX34043.1"
                     /translation="MTKRDYYEILEVQRNASEAEIKKAYRKMALKYHPDKNQGDKGAE
                     EKFKEAAEAYEVLSDPDKRERYNRYGHEGVRGQGGGMSMDDIFSHFGDIFGSAFGGGF
                     GGFSGFGDGARRRVNRGSNLRVKVKLTLEEIAVGVEKKIKVNKYVKCEACTGSGAQNG
                     SAYSTCGTCHGTGQVTRVTNTFLGQMQTTSTCPTCGGEGRLITQKCTSCGGDGIVKDE
                     EIISINIPAGVADRMQLSVSGKGNAGARGGINGDLIIIIEEKEHEELVRDGNNLIFEK
                     YISFPEAALGCQIEVPTLEGKARITLEPGVQSGKVLRLKAKGLPSIDRYGKGDLLVTL
                     NVWTPESLTKEEKRIMEELSQSLSFTPKPSKGQKNFFSRMREYFQ"
     gene            complement(9754..10368)
                     /locus_tag="PK345_03120"
     CDS             complement(9754..10368)
                     /locus_tag="PK345_03120"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HPX34044.1"
                     /translation="MIDEKRVDPEHTEGEIPIEKGNKSSENKKQDKSVGDSENAEKHT
                     LKKGAKAVKKYEDKIQELEEKLKEINGKYLRLFSDFDNFRKRTAKERLDLLNIASEEV
                     ILSLLPVIDDFERALAVVEKNNNNTDYEGIKLIYNKLTNILGKKGLSPINSKGELFDT
                     DFHEAFTIIPAPSEDMKNKVIDEIQKGYILNGKVIRFARVVVGQ"
     gene            complement(10501..11028)
                     /locus_tag="PK345_03125"
     CDS             complement(10501..11028)
                     /locus_tag="PK345_03125"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPX34045.1"
                     /translation="MPSRILTFLFLIFLSASTENLFAQSAVIGLEVGDIAPNISMPDT
                     KGEILSLYDLRGNIVLIHFWAAWCRPCRHENVELAELYKLFKDKRLKSENGFRIYSVS
                     LDKTRENWLSAIEKDELTWKEHVSVLKGWDSPAAIAFDIRAIPAGILIDGNGKIVAKN
                     PKIEQLQQILSERSQ"
     gene            complement(11063..12367)
                     /gene="murA"
                     /locus_tag="PK345_03130"
     CDS             complement(11063..12367)
                     /gene="murA"
                     /locus_tag="PK345_03130"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005641366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HPX34046.1"
                     /translation="MSSFVIQGGKKLNGSITPQGAKNEALQIICATLLTPEKVTISNI
                     PLIKDVKNLIDLIRDMGVKVGKVDRNTFTFEAIDINLDYLKSDTFRKKGASLRGSIMI
                     LGPLLARFGVGYIPQPGGDKIGRRRLDTHFIGLQKLGARFEHDSAKSLFKAEAPKLTG
                     TYMLLDEASVTGTANVLMAATLASGTTTIFNAACEPYLVQLSKMLNRMGAKITGIGSN
                     LLTIEGVERLNGCEHKLLPDMIEIGSFIGLAAMTQSAITINDVDCSQLGVIPAVFRRL
                     GIKIEENNNKLIIPEQDLYEIDTFMDGSIMTIADAPWPGFTPDLISIALVTAIQAKGS
                     VLIHQKMFESRLYFVDKLIDMGAQIILCDPHRATVIGLARKYQLRGLRMSSPDIRAGV
                     ALLIAALSASGESIIDNIDQIDRGYQDIDIRLKALGAEIVRV"
     gene            complement(12528..13178)
                     /locus_tag="PK345_03135"
     CDS             complement(12528..13178)
                     /locus_tag="PK345_03135"
                     /inference="COORDINATES: protein motif:HMM:NF025488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4290 domain-containing protein"
                     /protein_id="HPX34047.1"
                     /translation="MTDLEYNTQRKSIPLPEYGRNLQKMVTKVVNMKDREKRTRYAHQ
                     IIEIMIQINPTAKDSPELRHKLWDHLHIMSDFKLDIDSPFPLPDKEVLKTQPEQLSYP
                     DDNLKYNYYGRIIQNIIDVVAEMENGQEKDFHAKNIANQMKKSYLMWNRDTVEDSLII
                     QHLSELSNNKIKLPENFQLKRSSELVGHQQMNKRKGGSQGQQGKKRKRIKYNPQGK"
     gene            complement(13222..14298)
                     /gene="prfA"
                     /locus_tag="PK345_03140"
     CDS             complement(13222..14298)
                     /gene="prfA"
                     /locus_tag="PK345_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004334461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HPX34048.1"
                     /translation="MLEKLEAIKKKWKEIEQKMNEPSAMANMKLFIKLNKDYKDLQPI
                     VEGYDKLSLIVNNIESAKDVLNNEKDEEFRSLAKEELSVLIPQREELEEKLRLMLIPK
                     DPTDSKNSIVEIRAGTGGDEASIFAGDLYRMYVKFAESKGWKVEYLDSTDGTVGGFKE
                     VIFNIIGEDVYGQMKYESGVHRVQRVPVTEAQGRVHTSAASVVVLPEADEFDVVLNPA
                     DIRRDTFCSSGPGGQSVNTTYSAIRLTHIPTGIVATCQDEKSQIKNYNKALSVLRTRL
                     FELEYQKYLNEIASKRKTMVSTGDRSAKIRTYNYPQGRVTDHRIGLTLYNLQSIVDGN
                     IQELIDQLQLAENTERLKAEIEAA"
     gene            complement(14385..15764)
                     /locus_tag="PK345_03145"
     CDS             complement(14385..15764)
                     /locus_tag="PK345_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPX34049.1"
                     /translation="MNKLLSPSFIILFFSLFITRCIFAKGDKSFFHDPEKTYKVYTFD
                     IKENIAPPVWHLVKTALKTANEQEVDLILIHMNTYGGMLETADSIRTAILHSPISVWV
                     FIDNNAASAGALISIACDSIYMRSGANIGAATVVDQMGDAVPDKYQSYMRSMMRSTAE
                     AKGRDQRIAEGMVDPRIKIPGVSDSGHVITFTTSEAIRHKFAEGQAESLEDVLKVAGI
                     NNYKIIKHHLSFVDRIIRFLINPIVSGILIMIIVGGIYFELQTPGVGFPLAAAITAAL
                     LYFAPLYIEGIATHWEIIIFIVGVILIAVEIFVLPGFGVTGVLGIIMVLLGLALGMVG
                     SNGPEMWNLPFKELIVALFTVIIASLVSLAISFWISRKLFTTSRFGELALTTTQDKEI
                     GYTSAMASYEIMIGAEGIAKTILRPSGKIEIENETYDATAETGFIEKGDKVIVTNYIN
                     AQLFVRKLP"
     gene            complement(15974..16858)
                     /gene="fabD"
                     /locus_tag="PK345_03150"
     CDS             complement(15974..16858)
                     /gene="fabD"
                     /locus_tag="PK345_03150"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HPX34050.1"
                     /translation="MKAYVFPGQGAQFAGMGKDLYEKSSMAKELFEKANKILGFKITE
                     IMFDGTDDDLKQTKVTQPAIFLHSVILARVLKDDFKPDMVAGHSLGEFSALVANKSLT
                     FEDGLKLVSKRALAMQKACESEPSTMAAIIGLDDAIVENVLNETEELVVPANYNSPGQ
                     LVISGTIKGVEVACAKLIEAGAKRALILKVGGAFHSPLMEPARLELSKAIENVKFVKG
                     ICPIYQNVTAQPVTDPEIIKANLISQLTSPVRWTQIMKNMLADGAKEVVEVGPGTVLI
                     GLFKKVDRNLNVSSAVIP"
     gene            17210..18070
                     /locus_tag="PK345_03155"
     CDS             17210..18070
                     /locus_tag="PK345_03155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34051.1"
                     /translation="MKKSIFILALILTFFTSCNRKEVERLKQENQSLQNLINQKENEI
                     NSIILLLNEIEENLTGIRIREERIMKASSGIETNNNQIENIKSDVMAIDDLMKKNREN
                     LELLSQRLKATTGEKQQLEKMINNLNSQVDRKDKEIQKLVADLYDKNLQIHELYSSIT
                     QLETEKAEIEEVIDQQDKELHRAYWIIGNSKELKKSGIIVSSGGFLGIGKSNKLADQL
                     DPFDFNIIDIREQNIFPIEAKKAKLISLHPSDSYIMRKSDDGKKVISFEITRVEEFWK
                     NSKFMVLSLD"
     gene            complement(18126..18725)
                     /gene="folE"
                     /locus_tag="PK345_03160"
     CDS             complement(18126..18725)
                     /gene="folE"
                     /locus_tag="PK345_03160"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HPX34052.1"
                     /translation="MDGYEKIVFYNQEIIDRLSFHYHEILKLIGENPERDGLLKTPER
                     VAKAMQFLTHGYDLDPAEILRSAMFKEDYKQMVLVKDIEIYSMCEHHMIPFFGKAHVA
                     YIPNEYIVGLSKIPRIVDAFARRLQVQERLTTEIKDCIHKTLKPLGVAVVIEAQHMCM
                     SMRGIQKQNSVTTTSDFTGAFANEKTRAEFIHLIGSKLH"
     gene            19136..19912
                     /locus_tag="PK345_03165"
     CDS             19136..19912
                     /locus_tag="PK345_03165"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HPX34053.1"
                     /translation="MILTDTHTHLYLDHFDNDRDEMIKRAFSVGVRYMLLPNINSDSL
                     PGLLNLHSKYPKNIFPMIGLHPTNVKADWEEEIGYLMEHFEANKFYAIGETGIDLYWD
                     KTFIEKQKASLQFQIDLAKTSQLPIVLHIRDSFDEVFEVLNKNADQQLTGVFHCFTGN
                     LEQAERAIELGFMLGIGGVLTYKNSGLDKVVATIDMSHLLLETDSPYLAPNPYRGKRN
                     ESAYVRFIAARLAEIKGLTVAEVANITTENAIRLFKLPLS"
     gene            19940..21004
                     /locus_tag="PK345_03170"
     CDS             19940..21004
                     /locus_tag="PK345_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009132000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HPX34054.1"
                     /translation="MKLFSKSSVLVIYTGGTIGMIKDAETGLLIPFNFDKVHEYLPSQ
                     KLFGFNIESFSFDPVIDSSDMHPRHWINIAQLIYDQYENHNGFVVLHGTDTMAYTASA
                     LSFMLENLNKPVVLTGSQLPLGMLRTDGRENFVTALEIAAATIDGTAIVPEVAVYFEN
                     QLMRGNRTIKFNAEDFEAFRSPNYDPLATVGIHIKYNHSQIRKSNFKKLVLNTNLCTD
                     IAILKLFPGITKKTIEAILNTEGLRGVVLETYGTGNAPTEPWILNTFADAIKRGIVIL
                     NVTQCHGGRVEMEKYQTGFALKKIGILSGSDITTEAAVTKLMYVLGNYQKYDDVVMAL
                     TTPLAGEMNNKGAVDNVDFY"
     gene            21129..21216
                     /locus_tag="PK345_03175"
     tRNA            21129..21216
                     /locus_tag="PK345_03175"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21163..21165,aa:Ser,seq:gga)
     gene            21292..22104
                     /locus_tag="PK345_03180"
     CDS             21292..22104
                     /locus_tag="PK345_03180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010262025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MotA/TolQ/ExbB proton channel family protein"
                     /protein_id="HPX34055.1"
                     /translation="MKKLFAFLTIAAIFSFGIAVSVLAQDDTTSTEMTETELQDEAIE
                     EEVAVPTLEAEPEEQKEFHQVIKEQFIEGGWEFMGVILLTLIFGLALAIERIIYLNLS
                     TTNTTKLLKKVESALETGGVEKAKEICKDTRGPVASIFYQGLERYDEGIEIVEKSIVS
                     YGSVLMGRLESGLSWISLFIALAPMLGFLGTVVGMIIAFDNIAAAGDISPTIVASGIK
                     IALLTTVFGLIVAIILQLFYNYIVSKIESLVNSMEDSTISFIDIMTKHKSKE"
     gene            22120..22539
                     /locus_tag="PK345_03185"
     CDS             22120..22539
                     /locus_tag="PK345_03185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34056.1"
                     /translation="MKDKIATILRVILGLLLGVSLILFIVFYTKGEEFTNVVLIWAYI
                     LFALTALVTIIFPVINIILNPKSGKTVLIGLIGFAVLFIIAYSMASGYNEGTVYETFN
                     ISEAKSKFIGGMLNSTYILAALAVLAIVYSSISSVFK"
BASE COUNT         6872 a         4644 c         3857 g         7167 t
ORIGIN      
        1 aatccggagt taaagccaaa gcagggatta tgtttgtcag aatattaagt cctgaaaaaa
       61 ctaatattat taaagtattg aagcaaaatt aattgattag atatttcaat tttgaaaacc
      121 cgataaattc acccttatcg ggttttttta tctatttttt gggaacaagg ctcacgtagg
      181 atcaatacat ttcatttata ctccatacaa aagctctaat accaacttcc ctgttgattt
      241 catctacctt tttcaccctt tctaataggg gtttcacttt ttcttcttcc acaatagcaa
      301 taagcgccga attcatttcg ggccatgtat gcgttccttg ccgaggttct ccatccacgc
      361 ttccgcgtcc tttcacttcg ctccaccagc tatagccacg tattccgagc ttatctaaga
      421 tatattccac cctttcagta tgcgcttgat taaaaacgat aaatattgct ttcatactta
      481 gtcctttttt tgtaaaagtt aatcatccaa agcattaaat tgctttctta atttcatgat
      541 tttgtttcgt tcgcccgagc gtcccatcaa cccgtacatc accggtacaa tgatcattgt
      601 tatgattgtt gaaaaaacaa gcccgccgat tactgtaatt cccatcggtc gccatatttc
      661 ggatccttct ccagtgctaa aagccaacgg taccatccct aagattgtgg ttaaggcagt
      721 catcaataca gggcgaagcc ttgagcgtcc tgcagccaca atagcatcat aaagcctcat
      781 tcctctgtcg cgcatcaggt tggtaaagtc aaccaaaata attccatttt tcaccacaat
      841 tccaatcaac aacacagcgc caaggcctgc tactatgttc aacgttgagc cggtaataaa
      901 caatgcaagc attactcctg taaaagcaaa tggaatggag aacataatga taaacggcat
      961 agtgaacgat tcaaattgtg aagccattac tataaatact agaatcaaac tcatgagcat
     1021 caacaacatc aaatcctgaa acgactcctg ctgatcctca taagacccac ccacgttaac
     1081 aagtacatcc tgaggaacag gtacttcgtc ggtaagcttg attatttttt ttgccagttc
     1141 acttagcgaa ataccgttcg gtatagccga tacggttact atacgttctt ttcgtttatg
     1201 ttgaatagag ggcggccccc aatactcttc aatacttccg atctctttaa gttttatctt
     1261 cttaccttgt tggttcatta tagtcagctc ttcgagcaga gtaattgaat tgcgatattt
     1321 ttcggataat ctaatcctga tgtcgtattc ctctccttct tctttaaacc gcgatgcaat
     1381 cattccgttt accctgttac gaatcatggt agagactgtg gcgctattca atccatgaag
     1441 tgccatcttt tccctatcga gtaccacttg aagctcaggt ttgtcatttt tacggctgac
     1501 tacaatattt ttggcttcgg gcatattgct taatctctgg cggaactcct ctgctattct
     1561 gtttgtggta ttgaaatcat atccaaaaat ctcaatagct acattgcttt caccgcccat
     1621 gcccatacct gctccggttg ttgaaatatt gaatgttacc acttctggaa aagcttttac
     1681 aatatctctc atttcatcag caatatccca tactgttctt tcgcgttcgt ccacatgtgt
     1741 taaacgcatc ataacattaa taatgttaga cccagagccg ataaacagcg aaaacatacc
     1801 accctcatca tccgaacctg ttgagacatt aagcagttct acttccggaa atttttcctg
     1861 aataatattc tctaatttac gagctgtttt cattgtctct tcaacgcgtg ttcctgtttg
     1921 aagctccata ctaattgaca aacgtgattc gtcagtctgc ggcataaagt cagtaccaac
     1981 aaatttcaaa gagaacaatg atgcaataaa aacaatactt agacttatca acacaaaagc
     2041 tttatgacgt aaaacccatg taagcgtttt ggcataaaag tcgtcaagct tgttaagaag
     2101 cggctcaata gttcttccat agctaaattt tttcattttg acctcttttt tacgcaattt
     2161 caacaccctg ctcgacatca ttggagtaag cgaaattgca gcaaatgttg aagctacaac
     2221 agtaatcgta acaatccaac caagctgatt gaacatcatt ccggtcatcc ctcctaccaa
     2281 ggtaagggga aaaaaaacgg ctacaacaac cagtgttgta ataattaccg ctaaccacac
     2341 ttcattagta gcataaatag cggcttcgcg tggactactt cctctttcaa tatggcgggt
     2401 tatattttcg agtactacaa tggcatcgtc caccaccatt cctattgcaa tagagagcga
     2461 agtaagcgag attacattaa tagagttatc ggtaataaag agataaataa atgcgacaat
     2521 aagcgaaaca ggtattgtta gcgctataat aaatgtagct ctccaccttc cgagaaagac
     2581 cagaattacc aacactacaa agataaaggc atagatcagc gtttgcgaca agttgctgat
     2641 tgctccttta ataaatgaag aggtgtcaag aatctggttt atttgtatat cgggaggtaa
     2701 agtttttttt agctcgttga ggtttttatt tacctcacgc gctactttta ccgtatttgc
     2761 gcccgattgt ttcatcacga acatacgcaa ccctgttttt ccattgatct tttcatcgag
     2821 cgaaagatct ttcagcgaat cgcgtactac agccacatca cgcaaataaa ttggtattcc
     2881 gttataattt ccaacaacaa gattagccaa ttgcctgcta tcagtaaatt cgccctgaat
     2941 gcgtagctga taatccatat cacccatttt cacatttcct gagggcatat tcaggttttc
     3001 agaagcaata attcctccta tttgttctat tgtcagatta taagcatcca atcggcgggg
     3061 gtctgcctct atatagatta ttctttttgg cgcaccgatc atcgaaaccg aacctacccc
     3121 atcaatacgg tttagaggat taataatttt ctcttcaaga attttgtcca aaccaggata
     3181 gctttcattt gccgtaacgg cataaaaaac tatcggcatc atactggtat taaactttaa
     3241 aattgtaggt ctatcaacat cttccggcat gtaatcatac acacggtcaa tcacatcacg
     3301 catctgattg gtagcttcat caagattcga tccccagttg aacttcaaag ttatcaccga
     3361 taagttgtcc gttgaggttg aagtaacctc cttaacatta tcaaccgtgt tcaatgcatc
     3421 ctcaagaatt ttggttatat ttgtttcaat atcaagagcg cttgcgcccg ggtaggtagt
     3481 cattacagaa atatagggcg gatcaatctc aggatacagg tcaaccggca tcttaatcaa
     3541 tgcataaatc cccaccacaa tcacagcggt aaatatcatg aaagtagtga taggtctgtt
     3601 tattgcagat ttatatatac tcataaagtt attttttcag tattcagaaa ttaatttact
     3661 gttacacgca taccgtcaag cagacgtgcc tgtccgcgaa ttatcaactt atcaccctct
     3721 ttcaactcat ccgaaatcac ctccacaaat tcattatagc gttttccaag tgtaacaggg
     3781 attctttttg ctattccgtt ttcttctata aaaagatagc gatcgttaga gccttgaagt
     3841 tttaatatgg caattgatgg cagcacaatg gcctcttccc tatcgagatc aagcctaacg
     3901 cgacaaaaca ttcccggacg taacaattca ccggtattat ctaacgaaat ctcaatatta
     3961 aaacttcggc tggtaggatc tatagtagga taaatattaa acaccttacc ttcaaagact
     4021 ttatcgggat aaatgtcgga gataacggta gcgggcatac ctttgtttac cattgggaaa
     4081 tatttttccg aaacggccaa taaaatctta agacggtcaa tttgaactaa tgagagtatc
     4141 gccgattttc ctattgtagg tatgggagcg ccagaataca tctcgccggc ttcaaaatac
     4201 tttcctgaaa caatgccgta aaagggagcc gttaatttag tgttctcctt aagaaaattc
     4261 acattgcttt gggcaatatc taattgagcc ttcaactgat cgtattgctg acgcgggata
     4321 cttccggttt tattcagagt gtcaatacgt gcgaaatcag ttttaagtgt ttgaagctgt
     4381 acttctgcct gatggagctg agtttgatcc atttgaacta aaagttcacc ttctgcaacc
     4441 ctctgtccta cctttacaaa gatattgtca attctaccgg gcgctgccgg cgcataatgc
     4501 acctcttccc atgctaaaac agtggaggta tattcaacat cgcgggtaat ctcgcgtgtt
     4561 gagatgacaa gcacttcaac aggctctgct gccagctcct ccttttcttt cccgtcattt
     4621 tttatagtct cgcccgagca agctgcgagc atagtaaaag ctaaaccaaa ataaatgata
     4681 tgtttcattt ttaaattcct tttattaata tattgttccg taaatttttt ctaattgcaa
     4741 acgtgtactt agcacctgca tcatcgaatt aatgtaatct gattctgctt taaggtaatt
     4801 attatcggca tttatcaagt cgagagaaga taacatgcct tgcccgtatt tcaactttag
     4861 gtttaaatat acccggcgcg atacttcaat atttttttgt tgattttcaa atgtttcaag
     4921 tgcattttga aagttaaaac gcatctgtcg ttccgtaatg cgtaattgat cttctacaag
     4981 cgccttgttg ttctcaacgg tttttaaatc tactttcgcc tgctgtgttt gggaataacg
     5041 ctgcccactg gagaaaatcg gaatattcaa ctgcaaacca atcatgttgg gtggcgacat
     5101 atcaaaatca ggctttaaca acttgtgtgt ataacgataa aatgcaacta aagtaggcaa
     5161 agcatttgcc ttgttcatat ccaccatttt acgtgtaagc gtctcttgct gttgtatcat
     5221 tcgaaaatca atattccctt cgggattaaa ttcattgagc aacacatttt ccagagccga
     5281 atcttctatc aattgctcta acgtatcaag caagataagt tctgtatcaa tagaggtacc
     5341 taattgaagc cgcaacagat ttttggcaag ctctgcctgc ctctccgagg ccgaacgtac
     5401 atttaaaagc gaagtcaact gaacggaaag ctgatcaaga tcggtttgtt caatgacccc
     5461 gacatgcacc atcggttcag atttacggta cagatcctga agattttcaa catttttttc
     5521 taaaatagaa agcatctcct cagtgatcag aaccgcgtaa taggcatcgc taacggctgt
     5581 aataacatca agttccgttt tctctaaccc aagattagaa aactcttttg acaacgtagc
     5641 taactgaacg ccgacaatat aattaccgct aaagagtaac tgattcacat tcaagtacaa
     5701 attgctttga ggcttaatgt caatttcggc tgccggcatc ccttcctgaa aacgaataga
     5761 gatcttagcg ccaagggcgt tggaatagtc ggccgaagca ttaatttgcg gcaagcctgc
     5821 tgaaattgcc tcgcgaagtg cgtattgcgc tttgtcaata gcaaaatttg agtttttcat
     5881 agtcttatta tactccaatg cataagcacg agcatccata agacttaaac gaagtgactc
     5941 ctgcgaataa ctcagacttg cctgaaacaa caaaaccacg ataataacta aatatttaaa
     6001 tttcattttg atatgttttt attcttctaa ttttccaaaa aattcatcca tcagctcaat
     6061 tccttttggg gtggacaagc ccttgaaata ggggatgaaa atattttcca atatctccct
     6121 ttcattaggt tttaaaagta caatccgttg acttttatgc attgtaacca tcagatcctg
     6181 aaggaagctg tccactattt taatattgat cccttctttg aataccccct ctttaattcc
     6241 tctttccaac agaatatagg aaagtgaata acgctttaag tcatctgccc gggtataata
     6301 aagttttata atccttggga ggtagcgtgc gatatctttt ttgaacaaag gtgaatatga
     6361 atcgaggagt tcacgtttat gttttattat tttgaacatc gcctcaacaa catgctttga
     6421 ttcatttacg attttcgaca actcctcatt ctcattctga atgtaataca gcatggtgga
     6481 aataaccaaa tcatctttat gactaaaaca ctcataaatt gttcgctttg aaatgcctaa
     6541 gtccgaagca agggagtcca tcgtaacatt ttttatccct aatcccctaa atagctcacc
     6601 agcttttatc aaaattctct ctttcgtttc catatggcta agcaattttt ttcagggtgc
     6661 aaaactacaa aattattttc gttttatagt tttcatttga aaaaaatttt tttatctctc
     6721 ttaagaataa tccattaaac acgaattaag catctaacaa acaataatgc gggataaaaa
     6781 tattctctat cttcgaggca gttttaagtt tacaagtcat acagcataat tacaattcgt
     6841 ttcaataatg aaaaagtttt ttctacctgt tttattcttc ttattaattt tcgtacagtt
     6901 cgctttttat aaggcaaaga actttgcgat aactgaaatt aataatacga tatccggaat
     6961 taagattatc ggcctacaaa atgatttcaa tccatgtttt cttagtgatt ctctattcaa
     7021 cctaagtaac cagatgatgt gcattgatac gataatagaa gttaccacta atacacccgt
     7081 tggtagagga aatattaacg aaggtattgt tgctgctaat gtaattacca ttggaactgg
     7141 caatcctctt gaacttacct cgctcacctt ttcacttgat ggcacaaccg atatctccga
     7201 tataaagcaa attaacatca acttgagttt taattccaca cggacgtcca atagcctttt
     7261 acttaaaagt ttaaaaccac aaaaaaaaga tatcaccata atttgtaatc attcactaca
     7321 cgagggtaat aattttattt gggtttctgc tgatttacaa tcaaccgcta aagagggtaa
     7381 ttatttagct ggaaaaataa gctctgttac cattgataaa attgattatc cagtaacacc
     7441 ttctgccgaa aagagcaaca ggctgatatt tttagaacac aaattacttt tttccggtgg
     7501 cgattatggt tcggccaact ttagaatacc tgcactaacc acagccaacg acgggtcgct
     7561 gattgttgct gtagatgcac gggttgacag aaccggcgat ttaccaaata atattgacat
     7621 catgtatcgt cgcagcttcg atcagggcaa tagttggtca gacgcatctg tcatttgcga
     7681 tttcggaatg tatggcgcca gcgatcctgc cctggttgtt gataaaaaat ccggtgtaat
     7741 actttgtatg tttgcatctc atattgggct ttttcactcc acatttgata atccaatcag
     7801 ctttcaggta tgccgtagcg aggataatgg agtatcatgg agtaaacctg ttgatcatac
     7861 caattcaatt tatgcacccg gctggtatgc tgcctggtta gcatcgggaa gcgcacacca
     7921 gttgcgtaac ggaagaatag taggcgctat cggcgtaaga caagaggcag attatacaat
     7981 aagtaacttt atgatttata gcgatgatgt agggcttaca tggaattaca aatctgccca
     8041 agcttcagca ataggcgatg aggctaaaat tatggaatta gacaacggca acctgataat
     8101 gaacattaga aataagacac ccaattgccg aaaaattgtt gttagtgaag atggtggtga
     8161 tacctgggga gcgccctttt ttcagcacga actgattgat ccggcagtta acgccgattt
     8221 tatacgctac acttccgtac ttgacggtta cgacaaaagc cggttactct tttctactgc
     8281 ttcacatcca acaacacgaa aaaaccttac tttgtttatg agctatgacg aaggagttac
     8341 atggcctgtt tcaaaagtta tttacgaagg attatccgga tattcgtcac ttacaatatt
     8401 gaacgatggt actattggct gtgcttacga aaatggcgaa tttgaagagt atcagatcta
     8461 ttttgtcagg ctctcactcg attggctcac cgatggtaaa gacagttatc aacgccctaa
     8521 tgatatttta agaaagtgag taaataaaac cataggatcc tcagataaaa tgatcccttc
     8581 ttattgaaaa tattccctca tcctactaaa aaaattcttt tgtccttttg aaggtttcgg
     8641 ggtaaagctt aacgattgag aaagttcctc cataattctc ttttcttcct ttgtaaggct
     8701 ctctggtgtc caaacgttca aggttactaa caaatctcct ttaccgtaac gatcaatgct
     8761 tggtaaccct tttgctttca ggcgcaacac tttaccactt tgcacacccg gttccaaagt
     8821 aatacgtgct tttccttcaa gtgttggcac ttctatctgg catcccaatg ctgcttcagg
     8881 gaaagaaata tatttttcaa aaatcagatt attaccatca cgtactaatt cttcatgttc
     8941 tttttcttca ataataatga tcaggtcgcc atttattcct cctcttgctc cagcatttcc
     9001 tttgccgctc accgaaagtt gcattctatc tgctactccg gcaggaatgt taatactaat
     9061 tatttcttcg tctttcacaa tcccatcgcc gccacaggag gtacattttt gtgttattaa
     9121 tcgtccttcg cctccgcagg ttggacaagt ggatgtagtt tgcatttgcc cgaggaatgt
     9181 attagtaaca cgagttactt gaccagttcc atgacatgtt ccacaagtgc tatatgctga
     9241 tccgttttgt gcgcctgagc ctgtacaagc ttcacatttt acgtatttgt ttacctttat
     9301 ctttttctca acgccaacag ctatctcttc aagagtaagt ttaactttta cccgcaggtt
     9361 ggagccacgg ttaacacgac ggcgtgcacc atcgccaaaa ccactgaagc cgccaaaccc
     9421 gccaccaaaa gcgcttccga aaatatcacc aaaatgcgaa aaaatatcat ccattgacat
     9481 accgccgccc tgcccccgaa caccttcgtg tccgtaacgg ttatagcgtt cacgtttgtc
     9541 aggatcactc agcacttcgt aggcttctgc agcttcttta aacttttcct cagccccctt
     9601 atcaccctga tttttatcag gatgatattt aagagccatc ttgcgataag ccttcttgat
     9661 ctcagcctcg ctggcattac gctgaacttc aagtatttcg taataatctc tttttgtcat
     9721 attttcaatt gtttatgaca cacaggtatc caattactgt cctactacaa cccttgcaaa
     9781 cctaatcact ttgccattta aaatgtaacc cttttgaatt tcgtcaataa ctttgttctt
     9841 catatcttcg gatggcgccg gaataatggt gaaagcttca tgaaaatcag tatcaaataa
     9901 ctctcccttg ctattgatcg gtgaaagacc cttttttcct aagatatttg tgagcttatt
     9961 atatattaac ttaataccct cgtaatcggt gttgttatta tttttttcaa ccactgccag
    10021 cgcccgttca aaatcatcaa taactggaag taaacttaaa ataacctctt ctgatgctat
    10081 gtttaaaaga tcgagccgct ctttagccgt ccttttacga aagttatcaa aatcagaaaa
    10141 taatctcaga tatttaccat tgatttcctt taatttctct tctaactctt gtattttatc
    10201 ttcatatttt ttaacagctt tagcgccttt ttttaatgta tgtttttcag cgttttcaga
    10261 atcgccaaca gatttatcct gctttttgtt ttctgaactt ttattacctt tctctatggg
    10321 aatttctcct tccgtatgtt ccggatcaac cctcttttca tctatcataa tcttatgact
    10381 taataatcaa tattttgcaa cgataatttt tgaattaatt cctgtgccta tcaaaagtat
    10441 tgccatcaac tgttttaacg tcagaatgtc agaataatat ctttatctga tttaaaaagt
    10501 ttattggctc ctctcagaaa gtatttgctg caactgttcg atttttggat ttttagccac
    10561 tatctttcca tttccatcta ttaatatgcc ggcgggtatt gctctgatat caaatgctat
    10621 tgcagctggt gaatcccaac cttttaaaac gctgacgtgt tccttccatg ttaactcatc
    10681 tttttcgatt gccgaaagcc agttttcacg ggttttatcc aacgaaacac tatatatcct
    10741 gaacccattt tcagatttca atcttttatc tttaaataat ttgtaaagtt ctgcaagctc
    10801 aacattttca tgcctgcatg ggcggcacca tgctgcccaa aagtgaatca atactatatt
    10861 cccccgaaga tcgtaaagac taagaatttc accttttgtg tcaggcatac tgatatttgg
    10921 tgcaatgtcg ccgacttcaa gtcctattac cgcactctgt gcaaataaat tttcggttga
    10981 agcagataag aatatcaaaa aaagaaatgt caaaatacgc gatggcatgt aaaaattagt
    11041 tttgctgatt atataaaatc atttacactc tgacaatttc agctcccaat gcctttaacc
    11101 taatatcaat atcctgatat cccctatcta tttgatcaat attatctatt atactctctc
    11161 ccgatgctga taaagccgcg ataagcaatg caacgcctgc ccttatatcg ggcgaactca
    11221 ttctcaaccc gcgtaattga tattttcgtg ccagaccaat caccgtagcg cgatgtggat
    11281 cgcaaaggat aatttgcgct cccatatcta tcaacttatc aacaaagtaa agacgacttt
    11341 caaacatctt ctgatgtatc aacacactgc cttttgcctg aatagcggta accaaagcga
    11401 tgctgatcaa atcaggtgta aaaccaggcc agggagcatc tgcgatagtc attatgctac
    11461 catccataaa agtatcaatc tcatacaggt cctgttcagg gataataagt ttgttattat
    11521 tttcttcaat cttaattccc aatctacgaa acaccgccgg aatgactcct aactgactac
    11581 agtccacatc attaattgtg attgccgatt gcgtcattgc tgctaatcca ataaaactcc
    11641 caatctcaat catgtcgggt aacaatttat gttcacaccc gttaagcctt tccactcctt
    11701 caatagtcaa gaggttagag ccaattcctg taattttggc tcccatcctg ttcaacattt
    11761 tactaagctg tactaaataa ggttcgcagg cggcattaaa aatggtggta gtaccagaag
    11821 ccaatgtagc agccattaaa acattggcgg ttccagttac agaggcttca tcaagcaaca
    11881 tataagttcc tgtgagtttt ggcgcttcag ctttgaataa actttttgca ctgtcatgtt
    11941 caaatcgggc gcctaatttt tgcaaaccga tgaaatgtgt atcaagccga cgacggccaa
    12001 ttttatcacc ccctggctga ggtatatagc ccacaccaaa tcgtgcaagt aaaggaccta
    12061 aaatcattat tgatcccctg agacttgccc cttttttcct aaaagtatca gactttagat
    12121 aatcaagatt gatgtcaatc gcttcaaatg taaatgtatt acgatcaacc tttcctactt
    12181 taacacccat atcacgtatc agatcaatca gattttttac gtcttttata aggggaatat
    12241 tcgaaatggt tactttttcg ggcgttaaca aagtagcaca aataatttgt agagcttcat
    12301 tttttgcacc ctgaggtgta atgctcccat tgagcttttt cccgccttga ataacaaaag
    12361 aactcatatt taaaaatatt taatgatcag aaaatcgttt aactatcaca ttgtgagtaa
    12421 aataatgatt atcaaataag atctataatg atcttaacat cagtattaat aagccattac
    12481 ctctgattca gtaaagcgca ctatttacat cctcgatgtt ttgtctatta tttcccctga
    12541 gggttatatt ttatcctttt tctcttttta ccttgttggc cctgcgaacc gccctttctt
    12601 ttattcatct gttggtggcc taccaattca ctggatcgtt tcagctgaaa gttttccggc
    12661 aatttaatct tattattaga taattcggat aaatgctgaa taatcaaact atcttctact
    12721 gtatcacgat tccacatcaa ataggacttt ttcatttggt tagcaatatt ttttgcatga
    12781 aaatcttttt cctgcccatt ttccatctct gctacaacat caataatgtt ctgaattatt
    12841 cttccgtagt aattatattt aaggttgtcg tcaggatatg ataattgctc tggctgcgtt
    12901 tttagcacct ctttatccgg taatggaaac ggcgaatcaa tgtcgagttt gaagtcggac
    12961 attatgtgca gatgatccca taatttatgc cttaattccg gagaatcttt tgccgttggg
    13021 ttaatctgaa tcataatttc tataatttga tgggcatacc gagttctctt ttcgcggtct
    13081 ttcatattca ccaccttagt taccattttc tgaagatttc ttccgtattc tggcaaagga
    13141 atagatttgc gttgtgtatt atattccaga tctgtcattg ttataagttc gttttaattt
    13201 agttaataaa agttataggg attatgccgc ttcaatttca gctttaagtc tttcggtatt
    13261 ttctgctaat tgaagctggt caatgagttc ctgtatgttt ccatccacga ttgattgcag
    13321 attgtaaagc gtaagtccta ttcggtgatc tgttacacgg ccttgagggt agttgtaagt
    13381 tcgaattttg gcggagcgat cgccagtaga aaccatagtt ttacgtttcg atgcaatttc
    13441 gttaagatat ttctggtatt ccaattcgaa caatcgtgtt cttaacaccg ataaagcttt
    13501 attgtagttt ttaatttgcg atttttcgtc ctggcaagta gcaactattc cggttggaat
    13561 atgggttaac cttatagctg aataggtagt attcactgac tgtccaccag ggcctgagga
    13621 gcaaaatgtg tctctgcgga tatcagcagg gtttagtacc acatcaaatt catccgcttc
    13681 gggcaaaacc actaccgatg ccgctgaagt gtgtaccctt ccctgtgctt cagtaaccgg
    13741 cacacgctga acacgatgca cacctgattc gtatttcatc tggccataaa catcttcacc
    13801 aataatattg aaaatcacct ctttaaatcc gcctacggta ccatccgttg aatccaaata
    13861 ttccaccttc cagcctttag attctgcaaa ttttacatac atcctgtaaa gatcgcctgc
    13921 aaaaattgaa gcctcatcgc caccagtacc cgctctgatc tcaacgatag agttcttgct
    13981 atccgttggg tctttgggga tcaacattaa tcgcaacttc tcctccaact cttcacgctg
    14041 aggaatgaga acagataact cttctttagc cagagaacga aattcttcat ctttttcatt
    14101 attcaaaaca tccttagccg attcaatatt atttacaata aggctcaatt tgtcataacc
    14161 ttcaacaata ggttgtaaat ccttataatc tttattcagt ttgataaaca acttcatatt
    14221 agccatggca gatggctcat tcatcttctg ttcaatctct ttccactttt ttttaatagc
    14281 ttcaagtttt tcaagcatag gttttaaaaa tattttgcaa aattagcttt ttcgaaccga
    14341 ttggttgttt ttagataatt gtagattttc cttaaaaaaa tcacctatgg caattttctt
    14401 acaaaaagct gggcattaat ataatttgtt acaattacct tatctccttt ttcaatgaag
    14461 ccggtttctg ctgtagcatc gtacgtttca ttttcgattt caattttacc agatgggcgc
    14521 aatatggttt ttgcgattcc ttctgctcct atcataattt cgtatgaagc cattgcagat
    14581 gtataaccta tttctttatc ctgtgttgta gtcaaggcta attcgccaaa acgggatgta
    14641 gtgaaaagtt ttctactgat ccaaaaagat atagccaaag agactaaaga tgctattata
    14701 acagtaaata atgcgacaat cagttctttg aaaggtaaat tccacatttc aggcccgttg
    14761 ctacctacca ttcctaacgc tagtccaagt aaaaccatta taatacccaa tactcccgtt
    14821 actccgaatc ctggcaaaac aaatatttct acagctatta aaatgacccc aactataaat
    14881 atgataatct cccagtgagt tgcaatacct tcgatataga gtggggcaaa atagagcaat
    14941 gctgctgtaa ttgcagcagc aagcggaaag cctacgccag gtgtttgtaa ttcgaaataa
    15001 atccctccaa caattatcat aatcaggatt ccggaaacaa taggattgat gagaaatcga
    15061 ataatacggt caacaaatga cagatggtgt tttataattt tataattatt gataccagcc
    15121 acttttagta catcttcaag actttcggcc tgaccttcag caaatttatg acgtattgcc
    15181 tccgaagttg taaatgttat tacatgacca gaatcggaga ctccaggaat ttttattctt
    15241 ggatcaacca tcccttcagc tattctctga tcacggcctt ttgcctctgc agtagatcgc
    15301 atcatcgaac gcatatagga ttggtattta tcgggcacag catcacccat ctgatcaact
    15361 accgttgcag cgccaatatt agcgccagaa cgcatataaa tactgtcgca ggcaatcgaa
    15421 atcaaagcgc cggcagatgc tgcattatta tcaatgaaca cccaaactga aatcggtgaa
    15481 tgaagaattg cagtacgtat tgaatcagcg gtctcaagca tcccaccata agtgttcata
    15541 tggatgagaa taagatctac ctcctgttca tttgctgttt ttagagctgt ttttacaaga
    15601 tgccaaacag gtggtgcaat attctcctta atatcaaatg tgtacacctt ataggtcttt
    15661 tcaggatcat gaaaaaaact tttatcccct ttagcaaaaa tacagcgagt aatgaataaa
    15721 gaaaaaaaca gaataatgaa agaggggctt agtaatttat tcataatact catttttaac
    15781 cttttaataa catacaaaag taatgattgt ttttattttt ggataagaaa aataaaacat
    15841 atccactaaa agccttttta gaacacaaaa tcatcacgta gattttttac aaaatagacc
    15901 taccttcttt tactgaatct ctttttgttc aattaaaaga actgtggagc gaaaataaat
    15961 cttcttttaa ctgctaaggt attacagcgc ttgaaacgtt caggttgcga tctacttttt
    16021 tgaacagtcc tatcaataca gtgccaggac ctacttcaac cacttccttt gcgccgtcag
    16081 ctaacatgtt tttcattatt tgggtccagc gtaccggtga tgtcaattga gatatcaagt
    16141 tggctttaat aatttcggga tcggtaacgg gctgtgccgt aacattttga tatatcggac
    16201 atataccttt tacgaatttt acattttcta tggcttttga aagttctaaa cgggcaggtt
    16261 ccattagtgg tgaatgaaaa gcgccaccaa ctttaagtat caaggcacgt ttagccccgg
    16321 cttcgattag tttagcacaa gcaacctcta caccttttat agtgccagaa attaccaatt
    16381 ggccaggact attataattg gccggaacca ccagctcctc tgtctcgttc aacacattct
    16441 caacaatggc atcatcaagt cctatgattg cagccatagt agaaggctca ctctcacacg
    16501 ccttttgcat agccagcgca cgtttgctta caagctttaa accatcttca aaagtaagcg
    16561 atttattggc taccaaagcc gaaaactcac ctaacgaatg accggcaacc atatccggtt
    16621 tgaaatcatc ttttagtacc ctagctaaaa ttacggaatg taaaaaaatg gcaggctgag
    16681 taaccttagt ttgcttcaga tcatcgtcag tgccatcgaa cattatttcg gtaattttga
    16741 aaccaagtat tttattcgcc ttttcaaaca actccttggc cattgatgac ttttcgtaaa
    16801 ggtcctttcc catacctgca aactgtgcac cttgacccgg aaaaacgtaa gctttcataa
    16861 taattttggt taatcatctt atttgcgggc aaaaataaaa attaaattat tctaaggatt
    16921 ttcatttaaa agagaactaa acttaatacc aaattaagca cttgacattg ctttaacgac
    16981 taagtattta tataacctac ccaatgaaaa taataccgaa caaacattcc gaaatcactg
    17041 taacaaatca aaccttgtta tcaagtttac aatcattttc acccccctca ttataatgtt
    17101 accacaaaaa gagctgcaac cccatatgat ttcatcttat tattagacaa catttaagct
    17161 tttagctaaa aaaaatcgtt attaaatatc atcactcaat tttatgaata tgaaaaagtc
    17221 aatttttatt ttagcactta tccttacctt tttcacctcc tgtaacagaa aagaggtaga
    17281 gagactaaag caggaaaatc aaagcctaca aaatttaata aaccaaaaag agaatgaaat
    17341 caattccata attttacttc tcaacgaaat tgaagaaaac ctgaccggca tcagaatacg
    17401 agaagagaga attatgaaag cctcttccgg aatagaaaca aataacaatc agatcgaaaa
    17461 cattaaaagt gatgttatgg ccattgatga tctgatgaaa aaaaacagag aaaaccttga
    17521 gctacttagc caacgcctta aggcaacgac tggtgagaaa caacaactcg aaaagatgat
    17581 aaacaacctg aacagccagg tggacagaaa ggataaagaa attcagaagt tagtagcaga
    17641 tctttatgac aagaatttgc aaattcacga attgtattca tcaataacac aattagaaac
    17701 tgaaaaggct gaaatagaag aagttattga ccaacaagat aaagaattgc acagagccta
    17761 ttggattatc ggcaattcca aagagctaaa aaagagcgga ataatagtaa gcagcggtgg
    17821 tttcttaggt ataggaaaat caaataaact tgctgatcag cttgacccct ttgatttcaa
    17881 tattatagat atccgtgaac aaaacatctt cccgattgaa gcaaaaaaag ctaaacttat
    17941 ctcattacat ccatctgaca gctatattat gagaaagagc gatgacggta agaaagttat
    18001 cagtttcgag atcactcgag ttgaagagtt ttggaaaaat tcaaaattca tggtgctttc
    18061 acttgattaa aaccaagcaa tatcctagtg aacgttgatt tgtagtagct tttacagcta
    18121 tgttattaat gcagtttgga gccaataaga tgaataaact cagctctggt tttctcgttc
    18181 gcaaacgccc ctgtgaaatc ggaggttgtt gtaacagagt tttgcttctg tattccacgc
    18241 attgacatac acatatgctg tgcttcaatt accactgcta caccaagagg cttgagagtt
    18301 ttatgaatac agtcttttat ctcggtagtt aacctttcct gcacctgaag tcttcgggca
    18361 aaagcatcta caatacgtgg tattttactt aatcccacga tatactcatt aggaatatag
    18421 gctacatgag ctttaccaaa aaatgggatc atatgatgtt cacacatcga atatatttcg
    18481 atgtctttta ccagaaccat ttgcttgtaa tcctctttaa acatggcgct cctaagtatt
    18541 tcagcagggt ccaaatcgta tccatgtgtt agaaactgca ttgcctttgc aacccgctcc
    18601 ggagtcttta aaagaccatc cctttcagga ttttcgccaa ttaactttaa tatttcatga
    18661 taatgaaagc taagacggtc tatgatttcc tgattgtaaa aaacaatttt ttcgtaacca
    18721 tccattttat tttcatcagt catttctaaa agcatatcct ttttactctt ctgatttggt
    18781 attatattat tcataaaata taatttgcgg taaaaataca gttttcgtca aattgatgag
    18841 gtgtgattca acatgaaata aaaccataaa ttacaggtaa atacttaatt aaaaacgaat
    18901 taataaataa aaaaactttt gagctgaaca ttgattttat gaagccaatc ccgattaagt
    18961 gtttacaatt gactaatgcg taatctggat taaaacagta ctaagaaaca atcatcttgc
    19021 taataagttt ataatttttc gcccttactc tccaaatgct atcatggttc catgccaact
    19081 tttaaagaag aaacaaggtt ctttccactt gtaatgtttc gtttattttt gcaaaatgat
    19141 ccttaccgat acacacacac atttatatct cgatcatttc gacaacgacc gtgatgaaat
    19201 gatcaaacgt gcatttagtg ttggtgtacg atacatgttg ttgccaaaca ttaacagcga
    19261 tagcttacca gggctgctaa atcttcattc aaaatatcct aaaaacatct tccctatgat
    19321 agggcttcat cctactaatg ttaaagccga ctgggaagag gaaatagggt atttaatgga
    19381 acattttgaa gcaaataaat tttatgccat tggcgaaacg ggaattgatc tttattggga
    19441 caaaactttt attgaaaaac aaaaagcctc tttacaattt cagatagatc tggccaagac
    19501 aagtcaactg cctattgtat tacatattag agattctttc gatgaagttt ttgaggttct
    19561 gaacaaaaat gctgatcagc agctaacggg tgttttccac tgttttacag gtaatttaga
    19621 gcaggccgaa agagctattg agctgggatt tatgttaggg attggtggag ttttaacata
    19681 taaaaattct ggccttgaca aagtagtagc caccattgac atgagccatc tccttctcga
    19741 aaccgactcg ccctatcttg ctccaaatcc ttacagaggc aaacgtaacg aaagtgctta
    19801 tgtacgcttt atagcagcaa gattggcaga aataaaagga ttaacagtag cagaagttgc
    19861 taacataaca acagaaaatg ctattaggct ttttaaattg ccattaagtt gatttattag
    19921 tttttaattc aaaataatta tgaaattatt ttctaaaagt tcggttttgg tcatctatac
    19981 aggaggcact attggtatga ttaaagatgc ggaaactggc ctgttgattc cttttaattt
    20041 tgataaagtc cacgaatatc taccttcaca aaaattgttt ggctttaaca ttgaaagttt
    20101 cagcttcgat ccggtgatag actcttccga catgcacccg cgccattgga ttaatatcgc
    20161 ccagttaatt tatgaccagt atgaaaatca taatggattt gtggttttgc acggcaccga
    20221 tacaatggca tatacagcat ctgcactcag ttttatgctt gaaaacctaa ataaaccggt
    20281 tgttttaact gggtcgcagc tcccactggg aatgttacgt accgatggcc gggaaaattt
    20341 tgttaccgca ttagaaattg ccgcagctac cattgatgga acggcaatag tacctgaagt
    20401 agcagtttat tttgaaaatc agcttatgcg gggcaaccgt acaataaaat ttaatgccga
    20461 agactttgaa gctttccgct cacctaatta tgatccatta gcaacggtag ggatacatat
    20521 taaatataac catagtcaaa tcagaaagtc aaattttaaa aaattagtcc tcaacactaa
    20581 tttatgtacg gatattgcca ttttaaaact ttttccggga ataactaaaa aaaccatcga
    20641 agcaatttta aataccgagg ggctgcgtgg cgttgttttg gaaacttacg gcactggcaa
    20701 tgcccctact gaaccatgga tactaaacac atttgcagat gcaataaaaa gagggattgt
    20761 tattttaaat gttacacaat gtcatggtgg aagagtagag atggagaaat atcaaaccgg
    20821 ctttgctctc aaaaaaattg ggatacttag cggaagtgat atcactactg aggctgctgt
    20881 tactaaactc atgtacgtat taggcaatta tcaaaaatat gatgatgtag ttatggcgct
    20941 aacaacacct ttagctggag agatgaataa taaaggggca gtagataacg ttgattttta
    21001 ttgattttct aaaagtttga taatcagttg tataaaattg aaaaaatatt atttgttttt
    21061 gtattaaaaa aaatatttac tttagccgct cgaatttcag agagtcggtt tgaaacctta
    21121 ggtaatttgg agaggtggcc gagtggatga aggcgcacgc ctggaaagcg tgtgtacgcc
    21181 gaaagcgtac caagggttcg aatccctttc tctccgcaaa ttaatttttt ttgaattatt
    21241 aaaccttaac aaacaagaaa taatttatta tctaatcaac aaaagtaaat catgaaaaaa
    21301 ctatttgctt ttttaaccat tgctgcaata ttttctttcg gaattgcagt ttctgtctta
    21361 gcacaggatg ataccacctc aaccgaaatg accgaaaccg aacttcaaga tgaagccatc
    21421 gaggaggagg ttgcagtacc aacattagag gcggaaccag aagaacaaaa agagttccat
    21481 caggtaatca aggaacaatt tattgaagga ggttgggaat ttatgggtgt tattttgctt
    21541 acactcattt tcgggcttgc ccttgccatt gaaagaatta tctatcttaa tctttctact
    21601 accaacacaa ccaagttgct taaaaaagtt gaatcggcgc tggagactgg tggtgtagag
    21661 aaggcaaaag agatatgcaa agacactcgc gggcctgttg ctagcatttt ctatcaaggt
    21721 cttgaaagat acgatgaagg aattgagata gttgaaaagt caatagtaag ttatggaagc
    21781 gtacttatgg gaaggcttga gtccggtctt tcatggatat cactctttat cgcgcttgcc
    21841 cctatgcttg gcttccttgg tactgttgtc gggatgatca tcgctttcga caatattgct
    21901 gctgccggcg atatttcacc tacaattgtg gctagcggta ttaaaatcgc tcttcttact
    21961 accgttttcg gacttatagt agccatcatc cttcaattgt tctataacta tatcgtgtcg
    22021 aaaattgaaa gcttagtcaa tagcatggaa gatagtacaa tttcttttat tgatattatg
    22081 acaaaacata aatccaaaga ataaagaaag gatccgatta tgaaagataa aatagcaaca
    22141 attctcaggg ttatcctagg tttactgctt ggcgtatctc tcatactctt tattgtattt
    22201 tatacaaagg gtgaggagtt tacaaatgta gtacttattt gggcttatat cctgtttgca
    22261 ctaacagctc tggtaactat catttttccg gtaatcaata ttatcttaaa tcctaaaagt
    22321 ggtaagaccg tgcttattgg attaattgga ttcgctgttt tgtttataat agcatacagc
    22381 atggcttcag gatacaacga aggaacagtt tatgaaacat ttaatatttc tgaagcaaaa
    22441 tctaagttta ttggaggaat gcttaattca acctacattt tagcagcctt agctgtttta
    22501 gcgattgttt attcaagtat atcaagtgtt tttaaataaa
//
LOCUS       DAOOAI010000026        22249 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_3090_length_22249_cov_5.146436, whole genome shotgun sequence.
ACCESSION   DAOOAI010000026 DAOOAI010000000
VERSION     DAOOAI010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 22249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 22249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22249
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3090_length_22249_cov_5.146436"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..599)
                     /locus_tag="PK345_03190"
     CDS             complement(<1..599)
                     /locus_tag="PK345_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HPX34057.1"
                     /translation="MAQKSKNLKSSLLLLLAAIIWGFAFVAQRAGMAWVGPFTFNGIR
                     FLLGTLTLLPLVFISCRKNGKKELIAGGKQNKDLFTGGFVAGVVLFVAVTFQQIGIVY
                     TTAGKAGFITGLYVIIVPVIGFLFRQHISINVWFGAVMAVIGLYLLTITDGFALSSGD
                     SLVLVSALFFAIHILVIGKFSSKVNVIHLSAFQFAICSF"
     gene            816..2153
                     /locus_tag="PK345_03195"
     CDS             816..2153
                     /locus_tag="PK345_03195"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPX34058.1"
                     /translation="MKDLTIGNEGKLIVQFAMPMVYGNIFQQLYNVVDSIIIGHYLGK
                     ESLAAVGASFPIIFLLIALMIGFSMGFTIIIAQYFGAKNMVKVKQTIDTSYIIILFAS
                     LITTLLGISFSETIFRLTGMPDEVIPDAATYLNIYFAGSIFSYAFNGISAILRGLGDS
                     RTPLIFLIISTISNVFLDLLFIVVFKMGIAGAAYATIISKAGAVVAAIIYINKKFPIM
                     NISFRRYEFVWDIFKTSLRIGLPTGLQQTFVATGLIALYGIVNKFGTVTIAAYGVANR
                     IDTFASMLAMNFASALSAFVGQNIGANKPERVRTGLWTTWLITSGISIIFSIIVIFFG
                     KEMMRIFTPDTAVIEVGNGYLVIVGSFYILFSSLFVVNAVMRGAGDTLIPMFITLISL
                     WLIRVPASYFLAEKIGVTGIWWAIPFGWAIGLILTFIYYFTGRWKTKGVIKHI"
     gene            complement(2188..4257)
                     /gene="fusA"
                     /locus_tag="PK345_03200"
     CDS             complement(2188..4257)
                     /gene="fusA"
                     /locus_tag="PK345_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPX34059.1"
                     /translation="MNLSKLRNIGIAAHIDAGKTTLSERILFYTGTTRKLGEVHDGQA
                     IMDFMKQEQERGITIASAAISCSWKEFQINLIDTPGHVDFTVEVERSLRVLDGMVAVF
                     CAVGGVEPQSEAVWSQADRYRVPRIGFVNKMDRTGANYMDVIGQMNEYLDANAVPFQL
                     PIGAEENFEGMIDLVENRAIYFKEGEIVELPIPEELKPIAAKARQQMIEKLAESDERI
                     LELFLEDEPIPLEILKNAARKATLKLEITPVFCGAAYKNKGIQNLLDAVLDYLPSPLD
                     IGAVVGIDIYKEDKTHTRNPSVKDPFSALVFKLIHDPYVGQQTFVRIFSGSIRSGMQV
                     YNSSKQKYERVGRIFRIKAKERIEISEAGPGDIIALIGMKYSKTGDTLCNGDFPLLLE
                     SIHIPQTVIELKINPANRKEQSKLGEALAKLVNEDPSFGVRFNSETDETIISGMGELH
                     LEVIIDRLKGEFGVPVDVGEPSVAFRETITAEVECNYKHTKQTGGRGQYAHTVMRLEP
                     NEGNGYEFVDKIKGGVIPGEYIPSVNKGIQKTLDGGVLAGFPIVDVKVVLLDGSFHPV
                     DSSDMAFQTCASICFKNGFMKGNPSLLEPVMRIEINTPDEYIGSIVGDLNKRRGKIDS
                     MRRFRKGAQKLNGTVPLMEMFGYATALRNISSGRANYSMEFYKYVLVTSVIQDEILKK
                     LKELKEK"
     gene            complement(4408..5217)
                     /locus_tag="PK345_03205"
     CDS             complement(4408..5217)
                     /locus_tag="PK345_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34060.1"
                     /translation="MPIILMMISACSPERKLAREFIRNRDSTAVMLLMPSYILKSNLK
                     WWEVEDYDKMNDREKDSALYYNSTFLKEVDDDFLIARFKSSLQSGLMKYNIKPFTEDM
                     LLDFMEVGYRAYKVVLAQVELEEDIFQYHVEEVFFDTVLFYEDFDLNLISMNTWFEIT
                     PMNDPLSVNNVLYASGDMMDGIEGRFQNNLFSDDVKFNYNYFPIKTEDIYALTAMLGE
                     KYAGYINDYMLNEYIHRHFPDGERPKIYFSFDPSTGAVSPAKEERFTFIRP"
     gene            complement(5353..6564)
                     /locus_tag="PK345_03210"
     CDS             complement(5353..6564)
                     /locus_tag="PK345_03210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FprA family A-type flavoprotein"
                     /protein_id="HPX34061.1"
                     /translation="MKTIFDITTGVKWIGVQDPDLRTFDVVMETLYGTTYNSYFIEAD
                     KKTIIETTKEQFWDEYLYKIQQVCNPADIEYIVLNHTEPDHSGNLKNLLKIAPRATVV
                     GSGNAIRYLNDLMNISFPHIIVKDGQTLDLGNRQLRFISAPNLHWPDSMYTYLEDEKV
                     LFTCDSFGAHFSDEIMFEDKIVRRSEYEYSFKYYFDVILKPYSKFMLKAIEKISDLDI
                     DVIATGHGPILRENWRRYVALSKQYSEEALKQPERPKVFIPYVSAYQKTGQLAQYIAQ
                     GIKQAGDIDVEVIDIELTPLGELEEKISHASAFIIGSPTINQNILLPIYKCFALINPL
                     RDRGKLAGAFGSYGWSGEARSMIKTNLENLKLNFFDEGIFVKFTPATNDAEQAFAYGK
                     AFGERLLENHS"
     gene            complement(6770..7219)
                     /locus_tag="PK345_03215"
     CDS             complement(6770..7219)
                     /locus_tag="PK345_03215"
                     /inference="COORDINATES: protein motif:HMM:NF023238.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3332 family protein"
                     /protein_id="HPX34062.1"
                     /translation="MKRIRKFLALFIIASILFTQQSCIGSFQLTNSLYSWNKNDIGGK
                     WGQALVFFALVVIPVYQVALFADGVVLNTIEFWSGGNPLSMAPGEIETKIVKNGDDVY
                     KFTVEANLLQVEKLSGVNAGESGEFVYDQVDDAWSYFGSEGEFKLKR"
     gene            7434..9617
                     /locus_tag="PK345_03220"
     CDS             7434..9617
                     /locus_tag="PK345_03220"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPX34063.1"
                     /translation="MKKYIFVLSLAIVFGLNSLFASLRTDEGMWLPMFVERLNWTDIQ
                     KMGLQLTIDELYSINHSSLKDAVVGLAEGSAPNGFFCTGEIVSDKGLLFTNHHCAYDV
                     IQRHSSIQHDYLTDGFWAMNAEDEIPNPGLTASFLVRMEDVTEQVLSELDDEMTIEKR
                     AAKIREITSPIKEKASEDGKYDAVVKSFYDGSEYYLFVYQTFKDVRLVGAPPSSIGKF
                     GGDTDNWMWPRHTGDFAILRVYSAPDGSPADYSKDNVPLKPKPHLPVSIKGYEKDDYA
                     MIWGFPGNTERYLTSYGIDFAINQKNPAIIDVFGTMLDVMKKQMDADPAIRIELASDH
                     AGIANVWKYFIGQTRGLKRLDVKSKKEEMEKDFINWYKKNDETNEKYGNVLQNLQAAY
                     QQMEGTITPFYLIAIGSSNIECLNLSMQTVVMEEMLEKKKENKAQIELTIDALKETSE
                     EFYSDYNISMDRDMLSSMLSLFKKRLSLNQLPDIFATINAKFNGDINSYVDYVFKNSI
                     FTDKDRYNKFLSNPKVKTLKKDPINELSSSFNKIIASYQANLAEGQQNVSLNMRKFIA
                     GLREMNPSVKYYPDANSSMRFTYGTIKDYYPADAVHYDYITHAKGILEKEDPTNEEFI
                     VSPKLKELIKKGDFGIYGKDGDLVINFLSTNDITGGNSGSPVINGKGELIGIAFDGNW
                     EAMSGDIAFENELQRTISVDIRYVLFVIDKYAGAKHLIDELTLVK"
     gene            9831..10679
                     /locus_tag="PK345_03225"
     CDS             9831..10679
                     /locus_tag="PK345_03225"
                     /inference="COORDINATES: protein motif:HMM:NF015734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PASTA domain-containing protein"
                     /protein_id="HPX34064.1"
                     /translation="MSLRKFLLSRLFFKHLILSLIGGLILLWLIILFLGFYVKRGGTF
                     EIPDMVGMQLHELKQEIDPKAKFRYIVIDSIYDDRYEKGAIVQQDPPMGSKVKRGRKI
                     YLTVVAMQPETVNTPDFVDLTLRQALANIKAAGLTIERLTYIPNMAKNAVLDQKFEGK
                     NIKPGTKILKGSAIELILGKGLQDETVEVPYLIEKTLADAIALIHQSSLNVGYLSYSD
                     TTNNKIIKVYRQRPLASEEERVDFGAAVDLWVTGDTNFNFNAYKRENIEIIPDSDEDE
                     NIDDLF"
     gene            10686..12836
                     /locus_tag="PK345_03230"
     CDS             10686..12836
                     /locus_tag="PK345_03230"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34065.1"
                     /translation="MQKTIISILLFTSILFINFVTAQEILTGLSENPTIKSFIQSQGN
                     EKSSVIEQPDPLLLPFFDNFKKTSVFPDQSKWMDKNVFINNDFALFPPTWNVATFDAI
                     DSKGNLYPDANFVSFIADYLTSKPIRLDSIFSPQPKALSPADSVYFSFYYQPQGLAND
                     PQTRDSLVLEFGHYTDNYIFSYLDSITVDVAIFGIDTIYPGDFLFSPCDELLYIIAQD
                     TLFPGDQVTLPCDSVFVPETKWDRIWATKGMRLDSFLLVNNNQYFKQVMIPIIDSAYF
                     RNDFQFRFFNYASISTDNLQSWQSNCDQWNIDYVLLDKGRSRNDSTHKALTFVGQAPS
                     FLNQYQSMPYYQYSNDPTSVIKQRVDIYISNLDNGNQTANYYYEVKNDQGSFTFGFDA
                     GSDDLLPFNQAGYASNPSFAHPPVTTFFPPFGNRDSIYFDITHYLEGDLLLGLADTLR
                     FRQKFFNYYAYDDGTAEFGYGLTPTGAQLAYKFTLSRRDTLRAIQMYFNKTLTGANER
                     YFHLAVWGDLNGIPGDLLYIKENQKPIFGNELFEFQTYYLDEPLPVQGTFYVGWIQQT
                     SNNLNLGFDASNDVSDKIFYNVTGEWVNSSYKGALMIRPLLGKKIGEQPPEKATSANN
                     FLIVPNPSKDGKFEIQLQYLTPGNNKPQLVIPDNEREKQIIIEIFTLSGQKVFTSTYK
                     PEFALEHLKNGIYLVSIIDHYQRSHMVQKLVIAK"
     gene            12915..13952
                     /locus_tag="PK345_03235"
     CDS             12915..13952
                     /locus_tag="PK345_03235"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007217054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HPX34066.1"
                     /translation="MENVNLVMPDEQDDFYEHHRIQVDRGQSLIRIDKFLFGKLGNVS
                     RNKIQMAAKSGNILVNNKQEKSNYKVKPGDVISVLLPGPVKEFEIIAENIPINIVYED
                     DDIIVINKDAGMVVHPGHGNYTGTLINALAFYFQDNPDVLPALIHRIDKDTSGILLVG
                     KNELAQTILAKQFFEHSVNRKYFALVWGDMKMDAGTIEGSIGRNQTDRLQMTVYPDNS
                     QGKPAITHYKVIERFGYVTLVECTLETGRTHQIRAHFKYIGHPLFNDEKYGGDKILKG
                     TTFTKYKQFIINCFNILPRQALHAFLLEFTHPTTGKRMLFEAPLPNDFNKLIGKWREY
                     ARHKDTMNEYD"
     gene            14140..14298
                     /gene="rpmH"
                     /locus_tag="PK345_03240"
     CDS             14140..14298
                     /gene="rpmH"
                     /locus_tag="PK345_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HPX34067.1"
                     /translation="MSKRTYQPSRRKRVNKHGFRARMATKNGRKVLAARRAKGRKKLS
                     VSDEMLNK"
     gene            14782..15351
                     /locus_tag="PK345_03245"
     CDS             14782..15351
                     /locus_tag="PK345_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HPX34068.1"
                     /translation="MIKDRIRRSIEAKQELLNNHQLLETVHSVINVIIEAYRQGGKVL
                     FCGNGGSAADAQHLAAELSGRFYFDRPPLDAEALHVNTSYLTAVANDYSYDEVFARLV
                     KAKGKQGDVLIALSTSGNSPNVIKSLIAANERDIITIGLTGESGGKMKSYCRHLINVP
                     SADTPRIQEMHMLLGHIICELVEAKLFSK"
     gene            15348..16058
                     /locus_tag="PK345_03250"
     CDS             15348..16058
                     /locus_tag="PK345_03250"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HPX34069.1"
                     /translation="MISEAVILAGGFGTRLSSVLQGIPKPMAPVNGRPFLDYQMAYLA
                     FHGIKRVILAVGYLHDQIISHYGNRFGNIDIVYSIEHEPLGTGGAIKKALLESKENPV
                     LVLNGDTFFELDLREFHDFYRRRNTTLTIAMREVNDTSCYGSIEVDWNGRITGFYEKS
                     ESGKKGKINGGIYLIDKNFLFGFNLPDKFSIEKDFFEKIYRDHNIYAMLFKNYFIDIG
                     VPEEYSKTKDDFRHFRYF"
     gene            16030..16602
                     /locus_tag="PK345_03255"
     CDS             16030..16602
                     /locus_tag="PK345_03255"
                     /inference="COORDINATES: protein motif:HMM:TIGR01662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HPX34070.1"
                     /translation="MTLDTSDIFKDWALFLDRDGVINKRIVDDYVKSWADFVFCEGAI
                     EALKFFNTHFKRIIIVTNQQGVGKGLMTEEELQQIHEKMTTAIDQSGGRIDRIYSCTR
                     LKSENPFCRKPNPGMALAARRDFPDINFKFSIMVGDSISDLKFGKKMGMKTALIAPDF
                     KIARQFPKLTNLWFTSLIHFADFIRETKKY"
     gene            16640..18100
                     /locus_tag="PK345_03260"
     CDS             16640..18100
                     /locus_tag="PK345_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HPX34071.1"
                     /translation="MNHQLILIILLTYTLMLFIVGWITSKNADNESFFLNNRKSNWMV
                     VAYGMIGASLSGVTFISIPGWVSDTQFSYMVLVFGYLLGYLVIAKVLLPLYYNLGLTS
                     IYSYLDQRFGRWSYKTGAFYFLVSRLIGASFRIFLVINILQIFVFDKLGIPFWTSAVA
                     FVLLINLYTFRGGIKTIVWTDALQTTFMLASVIITAIFIKDELMMTTRTMFQSIAKSE
                     YSQMIFTNWQDQRFYLKQFFSGAFIAIVMTGLDQDMMQKNLSCRSLKDAQKNIISLSW
                     ALIPVNLLFLSLGTLLMIFANTKGIVIPGTSDTRFPEIAFNYLPSMAGIVFVIGLISA
                     AYSSADSAMTALTTSFTVDFLGIKRNPKYSVEKQKKIRQISHFGMALAFIIVIIMFKA
                     INNSAVINELFTIASYTYGPLLGLYAFGLFTKRQTRDRFTPFIAILAPLICYVLSKNS
                     EEWLWGYKFSFELLILNGLLMFLGLMIFSERKHVNK"
     gene            complement(18206..20611)
                     /locus_tag="PK345_03265"
     CDS             complement(18206..20611)
                     /locus_tag="PK345_03265"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPX34072.1"
                     /translation="MKKLFLLCFAIAFTLNVFAQISLSGVIRDSQTNEALSGAHLSLQ
                     PTFLTQISNERGEFGFTNLKPGVYTLKVTYLGYDNVVQVIEVKSDEFVDIGMSRRPIM
                     EEEVVVLASRVAGNSPVTFTNIEKEQIQKSNFGNDIPFLLATTPSLVATSDAGAGIGY
                     TGLKIRGTDITRINVTLNGVQWNDPESHSVYWVNLPDLASSIESIQIQRGVGTSSNGA
                     AAFGGSINIQTQSLRADPYAEISSIMGSFNTFKNAISVGTGLIDGKFTLDGRFSKINS
                     DGYVDRATSNLESIYFSAGYFTDKTLVKFNMISGREKTYQAWNGAPYDSLFTNRTFNP
                     SGLYYNGDGSIGYYDNETDNYKQDHYQFTCSRTLNRKMIMNLTAFMVKGFGYFENMKQ
                     NQKFSNYGLSDFIIGNDTIKRTDLVRRKYLDNAFYGGNFSFKYQHSQTLQSIIGGGYN
                     YYDGNHYGKIVRADFGENIPEDFLWYQNNGIKKQYNLFGKVNYEHNQRFNLFAEMQLR
                     AINYSINGIHDNLLDISQKHDYLFVNPKAGFVVPVNKRNQVYFSAAIANREPTRTDLK
                     DADLDNQPKEELLLNIEAGHTYTSNDFRFHTNLFFMDYKDQLVMTGKINRVGDPVFTN
                     VPNSYRAGVELAFGWKPTSNLNWQANLTFSKNKILNFTEYVDNWSPPFLQIETDLGTT
                     DISFSPSVTGSSILSYRILEGFDVALISKYVGKQYIDNTSNDDRKLDPYFVNDLMFRY
                     SISTGFIKGIDLSFTVNNIFDRKYITHAWIYRYFEDGGEYHQDGYFPQAGINILGGVF
                     LKF"
     regulatory      complement(20672..20769)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            20945..21529
                     /locus_tag="PK345_03270"
     CDS             20945..21529
                     /locus_tag="PK345_03270"
                     /inference="COORDINATES: protein motif:HMM:NF025492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4294 domain-containing protein"
                     /protein_id="HPX34073.1"
                     /translation="MSLKKYIFLIILLSGTFGYSQNSGLIVLPTTVVNGDTIPHIRLP
                     EAEIFGYKIFKNKREEYRYDRLVKNVRIVYPYAKLAGEMLREYEQILAAIPSESEKRK
                     IMKDLEDDLHKEYDDELKKLTITQGKILIKLIDRQTGDSSYDLVKEFRGAFRAFFYQT
                     FARIFGLNLKMKYDPEGEDKSIETIVRMIEKGVI"
     gene            21780..>22249
                     /locus_tag="PK345_03275"
     CDS             21780..>22249
                     /locus_tag="PK345_03275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34074.1"
                     /translation="MKKIKETRAYFITVPAQEEFENYRTSANRQLLLTMIYDENGNLL
                     EDHKYDNWGKLLGKTVYRYDNQNNRISEETFDPEDNLEERTTFEFENNKPVKGYIHYL
                     DDSKDEILFHYNQSGKLIMKEWISDEGEIDKTEQFVFSGDQLVLHKITEDGETI"
BASE COUNT         7173 a         4514 c         3904 g         6658 t
ORIGIN      
        1 aaaaatgaac atattgcaaa ctgaaaagca gaaagatgaa tgacattgac cttagaagaa
       61 aacttgccta ttactaaaat atgaattgca aaaaataggg cgctaacgag aaccaatgag
      121 tctccggaag acagagcaaa accgtcggtt attgttaata ggtataatcc gataacagcc
      181 attacagcgc caaaccaaac attaattgat atgtgctgcc taaacaaaaa tccgatcacc
      241 gggacaataa ttacatataa accggtaatg aaacctgcct ttcctgctgt tgtgtaaaca
      301 ataccgattt gctgaaacgt aacggccaca aaaagcacga cccctgctac aaatccaccc
      361 gtaaacaaat ctttgttttg tttaccacca gcaattaact ctttttttcc attttttcga
      421 caactgataa aaaccagcgg taacaatgtg agagttccta ataaaaacct gattccatta
      481 aatgtaaaag ggccaaccca agccattcca gcccgctgtg ctacaaaggc aaaaccccag
      541 ataatggccg caagcaataa tagcaaactc gatttcagat ttttactttt ctgtgccacg
      601 ttagtaattt aaagaaacgc aaaagtagaa ttattgaaat aaaatcgaga attaactcag
      661 atcaaacatt gaataactcg aaatctgtat ttacaattga acagatgtta tcgtaccgat
      721 gtttgtgatc atgcaatttg tgtttattca tctttttatt tacattacta tttttgcaaa
      781 ttagctgttg attaaacgac ggccgaaagc aattaatgaa agacttaacc ataggaaacg
      841 aagggaaact gattgtgcaa tttgccatgc caatggtgta tggtaatatt tttcaacagc
      901 tttataatgt agttgacagt ataataattg gccattatct tggcaaagaa tccttggcag
      961 ctgttggagc ctcttttcca ataatctttt tgcttattgc tttaatgatt ggcttttcaa
     1021 tgggctttac catcatcatt gctcaatatt ttggcgccaa gaatatggtt aaagtaaaac
     1081 aaaccattga taccagctat atcataatcc tttttgcctc attgataaca accttgttag
     1141 gtatcagctt tagtgaaacg atattcaggc ttacaggtat gcctgatgag gtaatccctg
     1201 atgcagccac ttatctgaac atctattttg caggctctat cttttcttat gcttttaacg
     1261 gaataagcgc tatactacgt ggtcttggcg attcccgaac accattaata ttcctgatca
     1321 tctctacaat atcaaatgta ttcctcgacc tgctttttat tgttgttttt aaaatgggta
     1381 tcgccggagc tgcatatgct actattatat ctaaagctgg cgcagttgta gctgccataa
     1441 tttacatcaa caaaaaattt ccaatcatga atatctcttt cagaagatat gaatttgtat
     1501 gggatatttt taagacaagt cttcgtatcg gactgcccac cggtttacaa caaacttttg
     1561 ttgccaccgg ccttattgcc ttatacggaa ttgttaacaa atttggcaca gttactattg
     1621 ccgcctatgg tgttgctaac cggattgaca catttgcctc tatgcttgcc atgaatttcg
     1681 catcagcatt atctgctttt gttggtcaaa atattggtgc aaataagccc gaacgtgtcc
     1741 gaacgggttt atggacaact tggttgatca cttccggcat ctcaataatc ttcagcatta
     1801 ttgttatctt ttttggaaaa gaaatgatga gaatttttac tccagatact gccgttattg
     1861 aagtcggaaa cggatatctt gttattgtcg gctcgtttta catactcttt tcatcccttt
     1921 ttgttgtcaa tgctgtaatg cgtggcgccg gcgatacatt gattcccatg tttatcaccc
     1981 ttatttcctt atggctaata cgggtgcctg cttcctactt tttagccgag aaaataggag
     2041 taaccggaat atggtgggca attcctttcg gctgggcaat aggattaata ctcactttta
     2101 tctattactt taccggtaga tggaaaacaa aaggcgtcat caagcatatt tgatcaaccc
     2161 tttatctatt tcattttaca aaagggatca tttctctttt aactctttca gctttttaag
     2221 tatctcatct tggattacag acgttaccaa cacatattta taaaactcca ttgagtagtt
     2281 tgcacgtccg ctactaatat ttctcaaagc tgtggcatat ccgaacattt ccatcaaagg
     2341 aaccgttcca ttaagctttt gcgccccttt ccgaaatctt cgcatcgaat caattttgcc
     2401 tctgcgtttg ttcaaatcac ccacaatact accgatatac tcatcagggg tattgatttc
     2461 aattcgcatg acaggttcca atagcgatgg atttcctttc ataaatccat ttttaaagca
     2521 tattgaagcg caggtttgaa aagccatatc agatgagtcc acaggatgga aactaccatc
     2581 aagaagtacg accttaacat caacaattgg aaatcctgcc aacacaccac catcgagagt
     2641 tttttgaatt cccttgttta ccgatgggat atactcgcca ggtattacac ctccttttat
     2701 cttgtcaaca aactcgtagc cattaccttc gttaggctca agccgcatga cggtatgtgc
     2761 atattgaccg cgtccacccg tctgttttgt gtgtttataa ttgcattcca cctccgccgt
     2821 aattgtttcg cgaaaagcaa cggaaggctc accgacatcc accggcacac caaattcgcc
     2881 tttcaatctg tcaattataa cttcgagatg aagctccccc attcctgaaa ttatggtttc
     2941 gtcggtctca gaattaaacc tgacaccaaa cgaaggatct tcattaacaa gttttgcaag
     3001 agcctctcct aatttgctct gctcttttcg gttggccgga ttaatcttta gttcaataac
     3061 cgtttgcgga atatgtattg attccaaaag caacggaaaa tcaccattac aaagtgtatc
     3121 gccggttttt gaatatttca ttcctatcaa tgcaataata tcgcccggtc cagcttccga
     3181 tatctcaatt ctctctttgg ccttaatccg aaatattcgc cccacccttt cgtacttctg
     3241 ttttgaagag ttgtaaacct gcataccact cctgatagag cccgaaaaga tacggacaaa
     3301 agtttgctgg ccaacataag gatcgtgtat cagtttaaaa actaatgccg aaaaggggtc
     3361 ttttacagat ggatttcgcg tgtgggtttt atcttctttg taaatatcaa tacctactac
     3421 tgctccgata tctaatggcg atggcaagta gtctaataca gcatcaagca gattttgaat
     3481 acctttattc ttataggctg ctccacaaaa aacgggagtt atctcgagct tcaatgtagc
     3541 tttgcgagca gcgtttttta atatttctaa agggataggc tcatcttcaa gaaataattc
     3601 caatattcgc tcatcagact cagccaattt ttcgatcatc tgttgccgag ctttagctgc
     3661 aatgggcttc aactcttcag ggatcggtaa ttcaacaatt tctccctcct tgaagtatat
     3721 cgcccgattt tccactaaat caatcattcc ttcaaagttc tcttcagcgc ctattggtaa
     3781 ttgaaatgga accgcattgg catcaaggta ttcattcatc tgccctatta catccatata
     3841 attggctcct gttcgatcca ttttatttac gaaaccaata cgtggcactc tataccggtc
     3901 ggcctggctc caaacggctt cgctttgcgg ctccacgcct ccaacggcac aaaagacagc
     3961 taccatccca tccaacacac gcaacgaacg ttcgacctct acggtaaaat caacatggcc
     4021 aggggtgtca atcaggttaa tctgaaactc tttccaacta catgatatgg ctgctgatgc
     4081 aatagtgata ccccgctcct gctcctgttt cataaaatcc ataatagcct gtccatcatg
     4141 cacctctcca agctttcggg ttgttccggt ataaaacaaa attcgctccg acaaggttgt
     4201 tttgccagca tcaatatggg ctgctatacc aatgtttcta agttttgaca aattcatatt
     4261 tagatgaaaa attatgaggg aaatttataa taaaagggtt tttaaaatga gtgtaaaagt
     4321 agccttaacg ggatttatgc tgtaaaaact tatcgagaaa gaaagagaca aataccaata
     4381 gttctttttg tgcgattcaa ttgatcgtta tggcctgata aaagtaaacc tctcctcttt
     4441 tgcaggcgaa acggcgcctg tggaaggatc aaaactaaag tatatcttag ggcgttcacc
     4501 atcagggaaa tgacgatgga tatactcgtt aagcatataa tcgttaatgt atccggcgta
     4561 tttttcacca agcattgccg taagggcata aatatcttct gttttgattg ggaaataatt
     4621 gtaattgaac tttacatcat ccgaaaacaa attattttga aatctgcctt caattccatc
     4681 catcatgtcg ccgctagcat acaaaacatt attcactgat agaggatcat tcattggggt
     4741 aatttcaaac catgtattca tactaataag gttgagatca aaatcttcat aaaaaagaac
     4801 ggtatcgaaa aagacctctt caacatgata ctgaaaaata tcctcttcca attctacttg
     4861 agctaaaacg actttatacg cccgatatcc tacttccata aagtcgagca acatatcctc
     4921 agtgaatggc ttaatattat acttcatcaa gccagattgc aggctgcttt taaatcttgc
     4981 aattaaaaag tcgtcgtcca cctctttcaa aaacgtactg ttatagtaga gtgcagaatc
     5041 tttctcccta tcattcattt tatcataatc ttcaacctcc caccatttta aatttgattt
     5101 caggatatac gaaggcatta gcaacattac tgctgttgaa tctctattcc ggataaactc
     5161 ccttgccaac ttacgttcag ggctacatgc gctaatcatc attagaataa tcggcatcgc
     5221 gataagcaac tttcttatta ccaacctttg ccaattatct aagagtaaag atactgtttt
     5281 taaaaaataa aaagatcgtc gcataataaa tttacccggc caaaaaaggt taacaaaatc
     5341 acaaaattga tattaactat gattttcgag tagcctttcg ccgaaagctt taccataagc
     5401 aaaggcttgt tcagcatcgt tagttgctgg tgtaaatttc acaaatatac cctcatcgaa
     5461 gaaattgagt ttaaggttct caaggttggt ttttatcatg ctacgtgctt cgccactcca
     5521 cccatacgaa ccaaaagctc cagcaagttt tcctctgtcg cgcaaagggt tgatcaaagc
     5581 aaagcattta tatatcggca aaagaatatt ttggttgatg gtaggcgagc cgattataaa
     5641 cgcagatgcg tgggagattt tctcctctaa ctcacctaat ggcgtcagtt cgatatcaat
     5701 aacctctaca tcaatgtcgc ctgcttgctt aataccctga gcaatatatt gagccaactg
     5761 tccggttttt tgataggcgc tgacgtatgg aataaaaact tttggtctct caggctgctt
     5821 tagagcttct tcagaatatt gtttcgaaag agctacatac ctcctccagt tttctcttaa
     5881 aattggccca tgacctgtgg caataacatc aatatccaaa tcacttatct tttctattgc
     5941 tttgagcatg aatttgctgt agggcttaag aataacatca aaataatact taaatgagta
     6001 ttcgtactcc gatcgcctga caattttatc ttcgaacata atctcatcgc taaaatgcgc
     6061 accaaacgaa tcgcaggtga aaagaacttt ttcatcttcc aaataggtgt acattgagtc
     6121 tggccaatgg aggttgggcg cgctaataaa ccgaagctgt ctgttgccca aatcaagtgt
     6181 ttgcccatct tttactatga tatgaggaaa actaatattc atcagatcat tgagatatct
     6241 aatggcgtta cctgatccaa caactgtcgc acggggagct atcttcagca gattcttaag
     6301 attacctgaa tgatcaggct ccgtgtggtt caaaacaata tattcgatat cagcagggtt
     6361 gcacacctgt tgaattttat atagatactc atcccaaaat tgttcttttg tggtttcgat
     6421 aatagttttc ttatcagcct cgataaagta ggagttataa gtggtgccat aaagtgtttc
     6481 catcacaaca tcgaatgttc gtaaatcagg atcttgcacc cctatccact taactccggt
     6541 agtaatatca aaaatagttt tcatgttctt ttatctattt aatttttaac tttttacata
     6601 aaaaattacc gctacattta aaaaaaaatg aattatgtca attttaaagt acaggtttta
     6661 ggcaaataaa catattattt aaatatgctt catgattaag tataaaacac gaagcgttta
     6721 ctaaccctcc cccccttcaa caaccaattc cgcaaaaaaa gtaatacctc tatcgtttaa
     6781 gtttgaattc gccttctgaa ccaaaataac tccaggcatc atctacctga tcgtaaacaa
     6841 actcgccgct ctctccagca tttacgccag atagtttttc aacttgcagc aaattagcct
     6901 caacggtaaa tttataaaca tcatctccat ttttaacaat ctttgtctct atttcacccg
     6961 gagccatgga aagcgggtta cctccactcc aaaactcaat agtattaagc acaacgccat
     7021 ccgcaaaaag ggcaacttga tataccggta tcactaccaa tgcaaaaaat actaaagcct
     7081 gcccccactt tccaccaata tcatttttgt tccagctata aagtgagttg gtgagttgaa
     7141 atgatccgat acaactttgt tgagtaaaca gtatactggc aataataaac agagctaaaa
     7201 actttctaat ccttttcata attgataaaa tttaaatttg cttataataa attactattc
     7261 aatttcttta ataaaaacta tttcaaaaag acaattttta aagatggggc aaaagtactt
     7321 tttttgaatt tgattgcacc atgattaaac taatgagtag aacaaaattc aatacttttg
     7381 ccgcagcaac taaacactaa tttttttaaa aacataatat caacacattt aacatgaaaa
     7441 aatacatttt tgttctttct ctggctattg ttttcggcct aaatagcctt tttgcctccc
     7501 tcagaaccga cgagggtatg tggttaccaa tgtttgttga acgcttaaat tggaccgaca
     7561 ttcaaaagat gggtttgcaa cttacaattg atgagcttta cagcatcaat cattccagct
     7621 tgaaagacgc cgttgtaggt cttgccgaag gcagcgcgcc aaatggcttc ttctgcaccg
     7681 gagagatcgt ttcggataaa gggcttctgt ttacaaacca tcactgcgcc tacgatgtca
     7741 ttcagcgcca tagctctatt cagcacgatt atcttactga tggcttttgg gcaatgaatg
     7801 ctgaagatga aattccaaat ccagggctga cagcctcctt tctggtacgg atggaagatg
     7861 ttaccgagca agtgctatca gagttagacg acgaaatgac cattgaaaaa cgtgcggcaa
     7921 aaatcagaga aataacctct ccgatcaaag aaaaagcctc cgaagacggt aaatatgatg
     7981 ccgttgtaaa gagtttttac gacggtagcg agtattatct ttttgtttat caaaccttca
     8041 aagatgttag gttggtgggc gctccgccat cgtcaatagg caaattcgga ggtgataccg
     8101 ataactggat gtggcctcga catacggggg attttgccat attaagggtt tatagcgcac
     8161 cggatggctc tccggccgac tattcgaaag ataatgtacc cttaaaacca aaaccccacc
     8221 ttcccgtttc aatcaaagga tatgaaaaag atgattatgc tatgatatgg gggttcccgg
     8281 gtaatactga aagataccta acctcctatg ggattgattt cgccataaat caaaagaacc
     8341 ctgcaattat tgatgtattc ggcaccatgc ttgacgtgat gaagaaacaa atggatgctg
     8401 atccagccat acgtattgaa cttgcttctg atcatgcagg catagcaaat gtatggaaat
     8461 attttatcgg tcaaacacgt ggcctgaaaa gattagatgt aaaaagcaaa aaagaggaga
     8521 tggaaaagga ttttataaac tggtataaaa aaaatgacga aaccaacgag aaatacggga
     8581 atgtgctaca aaaccttcaa gctgcttatc aacagatgga gggaacaatc acaccatttt
     8641 acctaatcgc aatcggatcg agtaatattg aatgcctaaa tctgtcaatg caaactgtgg
     8701 taatggaaga aatgctggag aagaaaaaag aaaataaagc gcaaattgag cttactattg
     8761 atgcattgaa agagacttca gaagaatttt atagcgatta taatatttca atggatcgcg
     8821 atatgttatc ttcaatgcta agtctgttca aaaaaagact atcgctaaat caattgcccg
     8881 acatctttgc aacaataaac gccaagttca atggcgatat aaattcttac gtagattatg
     8941 tttttaaaaa ttccattttt accgataagg atcgctacaa taaattttta tcaaatccta
     9001 aggttaaaac cctcaaaaaa gatcccatca acgaactctc ttcgtcattt aacaaaatca
     9061 tagccagcta tcaagctaat ttggccgaag gacagcaaaa cgttagttta aatatgcgca
     9121 aatttattgc cgggttgcgc gagatgaatc cttctgttaa atattaccct gatgccaact
     9181 cttcaatgcg tttcacctat ggaacaataa aagattacta ccctgctgat gctgtacatt
     9241 atgattacat aacacatgca aaagggatat tggaaaaaga agatcctacc aacgaagaat
     9301 tcattgtttc gccaaaacta aaagagctga ttaaaaaggg cgatttcggg atttatggca
     9361 aagacggaga ccttgtaatt aacttcctat ccacaaacga tatcactggc ggcaactccg
     9421 gcagccctgt tattaatggt aaaggcgaac taatcggaat tgcctttgat ggcaactggg
     9481 aagcaatgag cggcgatatc gcatttgaaa atgagctaca acgtactatc agtgttgaca
     9541 tacgctatgt actatttgta atagataaat acgccggagc aaagcatctt attgatgaac
     9601 tgacattggt aaaataaata tccgttccga caaagcagga agcacgcaaa atatctaagt
     9661 cgaattgtta attaaagcct tgcggaataa caacatattt tgagctttca agccattttc
     9721 aaattaagca tacaatcact ataagacgat tagcaataaa acaaaagctt ttcagtttaa
     9781 taaacagcta ttttgcagct aatctatttt catacaattt agtaaactga atgagtctaa
     9841 ggaaattctt actaagtaga ttgtttttca agcacctgat actctcttta ataggtggtc
     9901 taattttgct ttggttgatc attctttttc ttgggtttta cgttaaacgt ggcgggacat
     9961 ttgaaatacc tgacatggta ggaatgcaac ttcacgaatt aaaacaggaa attgatccta
    10021 aggctaaatt cagatacata gtgattgatt ccatttatga cgaccgatat gaaaagggcg
    10081 ctatcgttca acaggatcct ccaatgggtt caaaagtaaa aagaggcaga aagatttatt
    10141 taacagttgt tgccatgcag cccgaaacgg ttaacactcc tgatttcgtt gaccttacgt
    10201 tacgtcaggc gcttgccaac ataaaggcag ccggattaac catagagcgc ctgacataca
    10261 ttcccaacat ggcaaaaaac gccgttttag accaaaaatt tgaaggaaaa aatatcaaac
    10321 cggggaccaa aattctaaaa ggatcggcta ttgaacttat actcggaaag ggcctgcaag
    10381 acgaaactgt ggaagtgccg taccttatag aaaaaactct ggcagatgcg atagctctta
    10441 tccatcaatc atcgttaaat gttggttatt tgagttattc cgatactacc aacaataaga
    10501 ttataaaagt ttacagacaa cgacctctgg cttcagaaga agagagggtt gactttggcg
    10561 ctgcagttga cctatgggta acaggcgata caaattttaa ttttaatgct tacaaaagag
    10621 agaatattga aataatacct gattcggatg aagacgaaaa tattgatgat ctgttttaaa
    10681 aagagatgca aaaaacaata atctccatat tattatttac ttcgattctg ttcatcaatt
    10741 ttgtaacagc gcaggaaata cttaccggtc tttcggaaaa ccctactata aagagtttta
    10801 ttcaaagtca gggaaatgaa aaatcttcgg tgatagagca acccgatccg ctgttacttc
    10861 catttttcga caattttaaa aagacgtcag tctttcccga tcaatccaaa tggatggata
    10921 aaaatgtatt catcaacaac gattttgctc tattccctcc cacgtggaat gtagccactt
    10981 tcgatgccat tgacagcaaa ggcaatctat atcctgacgc taattttgtt tcgtttatcg
    11041 ccgactatct gacatcaaaa cccatccgtc tcgattctat tttttcgcct caacctaaag
    11101 ccttgtcacc cgctgattct gtctatttca gcttttatta ccagccgcaa ggccttgcga
    11161 acgaccctca aacccgggat tcattagtgc tcgaattcgg ccattatact gataattata
    11221 ttttttccta tctcgattcc atcactgtag atgttgccat ttttggcatt gacacgattt
    11281 atcccggtga ttttcttttt tcaccctgtg acgagcttct atatatcatt gcgcaagaca
    11341 cgctttttcc aggagatcaa gtaaccttgc cctgcgattc agtgtttgtt cccgaaacaa
    11401 aatgggacag aatatgggca acaaaaggaa tgcggctcga cagctttcta ttagtcaata
    11461 ataatcagta tttcaagcag gtaatgatcc ctatcattga ttctgcctac ttcagaaatg
    11521 atttccaatt taggtttttt aattatgcca gcatctcaac cgacaacctc cagagctggc
    11581 aaagcaattg cgatcaatgg aatattgatt acgtccttct tgacaaaggc agatccagaa
    11641 atgattcaac acataaagcc cttacattcg taggacaagc tccctccttt ctgaatcaat
    11701 atcaaagcat gccttattac caatattcaa atgatccgac gagtgtaata aaacaacggg
    11761 ttgatatcta tatcagcaat ttggataatg gaaaccagac ggccaattat tattatgaag
    11821 taaagaatga ccaaggtagc tttacctttg gctttgatgc gggtagcgac gacctgcttc
    11881 cttttaatca agcgggatac gcctctaatc catcatttgc ccaccctccg gttacaactt
    11941 ttttccctcc tttcggaaat cgcgacagca tctatttcga cattacccac taccttgaag
    12001 gtgatttact tttaggcctt gccgacactc ttcgtttcag gcaaaaattt ttcaactact
    12061 atgcttatga tgacgggact gctgaatttg gttacgggct taccccaaca ggagctcagt
    12121 tagcatacaa attcacttta agtcgtagag atacactgag agctatacag atgtacttta
    12181 acaaaacact taccggcgcc aacgaacgat attttcatct tgctgtgtgg ggcgacttaa
    12241 atggtatccc cggcgacctg ctttacatta aagagaacca aaaaccaata ttcggcaacg
    12301 aactctttga atttcaaact tactaccttg atgagcctct tcctgtacaa ggtacgtttt
    12361 acgtcggatg gattcagcaa acaagcaata acctaaacct cggattcgac gcatcgaatg
    12421 acgtctccga caaaatattt tacaatgtta ccggtgaatg ggtaaattct tcatacaaag
    12481 gggctttgat gataagaccg ttgctcggta aaaaaattgg tgaacagccg ccagaaaaag
    12541 caacaagcgc caacaacttc ttaattgtac caaacccatc aaaagatgga aaatttgaaa
    12601 tacaactcca atatctgaca ccaggtaaca acaaacctca attggtaatt cctgataacg
    12661 aaagagaaaa acaaataatt attgaaattt ttactctctc aggtcaaaag gtttttacaa
    12721 gcacctacaa accagagttt gctctggaac acctgaaaaa cggcatctat ttagtcagca
    12781 ttattgatca ttatcaacga tcgcacatgg tacaaaaact cgtaatagct aaataaattt
    12841 aaaaatcagg gttttctgaa ttgtcagcaa accaccaaat aactataaaa tcctatcgaa
    12901 ctaagccaaa ataaatggaa aatgtcaacc ttgtaatgcc tgatgaacag gatgatttct
    12961 acgaacacca caggattcaa gttgaccgtg gacaaagctt aattaggatt gataaatttc
    13021 tttttggcaa acttggcaac gtctcccgaa acaaaatcca gatggctgca aagagtggca
    13081 atattctggt aaacaacaag caggaaaaat caaattacaa agttaaacca ggggatgtca
    13141 tttccgtttt actgcctgga ccagtaaaag agttcgaaat aattgccgag aatatcccaa
    13201 ttaatatcgt ttacgaggac gacgacatta ttgtaattaa taaagatgcc ggaatggtgg
    13261 tacaccctgg acatgggaat tataccggaa cactgataaa cgcccttgca ttttactttc
    13321 aggacaatcc tgatgtatta cctgcactga tacatcggat tgacaaagac acatcaggta
    13381 ttctgcttgt tggaaaaaac gaattagcac aaactatact tgccaaacag tttttcgaac
    13441 atagcgtcaa ccgcaaatat tttgcattag tgtgggggga tatgaaaatg gatgccggta
    13501 caatagaagg aagcattggc cgcaaccaaa ccgaccgcct acaaatgaca gtttatcctg
    13561 acaattcgca agggaaacct gccatcacac attacaaagt tatcgaacga ttcggttatg
    13621 taactttagt tgaatgcaca ttagaaacag gacggacaca tcaaataagg gctcatttca
    13681 aatatattgg tcatccattg ttcaacgatg aaaaatatgg tggcgataaa attctaaaag
    13741 gaacaacatt taccaaatac aaacaattta taatcaactg tttcaatatc cttccgcggc
    13801 aggctttaca cgccttttta cttgaattta cacaccccac aacaggtaaa agaatgctat
    13861 ttgaagcgcc actacctaat gattttaata aactcatcgg aaaatggcgt gaatatgcac
    13921 gacataaaga tacaatgaac gaatacgact aaccccaaat gttatccttt taacaaagga
    13981 acaatcgtta aaactttaac tttctctaat tttaagtaac gttcaatatg aatttcttca
    14041 gaaaactctt tataaaaaat gaaaaaagaa aaaaattgaa gaaaatgttt ttaatctaaa
    14101 aaatcatact acttttgcac cccaaaataa gatagaacga tgagcaaaag aacttaccag
    14161 ccttcgaggc gtaaaagagt caacaaacac ggtttcagag ccagaatggc aactaaaaac
    14221 ggccgtaaag tacttgcggc acgtagagca aagggcagaa aaaaactttc cgtttctgac
    14281 gaaatgctta ataaataata attaagcaaa caccacatac tataatatca gaaaagtagc
    14341 taatacacaa tcctttccca aacatgacaa aagccccgct tagtaattag cttggcggga
    14401 ttttattttt taaagaattg agtctgctcc gcaaatagag tttttaaaat atccgattaa
    14461 agtatttgaa cgaattatag aatttcaatg atttataaat tttataattg tctatgggcg
    14521 ctaaataacg tcgaagtgtc agtggctata tttacttttc gcaatggaat tgatcttaac
    14581 tttaaaggtc ggccaaactc ttaataccca cttcggcgag ttcacccatt attcaaaact
    14641 attcccggct taattcaatg cttagtcttt aaaacatccc taggcttgca ttatcaaaga
    14701 tagaaacagc caaaattgat ttttcacatc tttagcaaaa tcattgattt cattactttt
    14761 gcattcgaat aattaatttt tatgataaaa gataggataa ggcgatcaat tgaagccaaa
    14821 caggagctat tgaacaacca tcaacttctt gaaacggtac attcggttat taacgttata
    14881 atagaagcct atagacaggg tggaaaagtt ctcttttgcg gcaatggcgg tagcgctgcc
    14941 gatgctcaac atcttgccgc ggagctttcg gggcgttttt atttcgacag gccaccactt
    15001 gatgccgaag cgctccatgt caacacatcc tacctcactg ctgtagcaaa cgattactcc
    15061 tacgatgaag tttttgcacg tcttgtaaaa gccaaaggta aacaaggcga tgtactgatt
    15121 gcactttcaa cttccggaaa ctcacctaac gtaataaaaa gtctcatcgc cgctaacgag
    15181 cgcgacataa tcactattgg cctgactggt gaatccggcg gcaaaatgaa atcatactgt
    15241 cgccatctca tcaatgtacc ttctgccgat acaccacgga ttcaagaaat gcatatgctt
    15301 ctggggcata ttatctgcga actggttgaa gctaaacttt tttcaaaatg atcagcgaag
    15361 ctgtaatatt agccggaggc ttcggaacac gcctgagcag cgttttacag ggcataccaa
    15421 aacctatggc tcccgttaat ggccgaccat tcctcgatta ccagatggcc tatctggcct
    15481 tccacggtat aaaaagggta atactcgccg ttggatatct tcacgatcaa attatttccc
    15541 attacggaaa tcgttttgga aatatagata ttgtttattc aattgaacac gagccgcttg
    15601 gcaccggtgg tgcaataaaa aaagcgcttt tagaatcaaa agaaaacccg gtgttggtac
    15661 taaatggtga tacctttttt gagcttgacc taagagaatt tcatgatttt taccgtcggc
    15721 gcaatactac tctaacaatt gcaatgcggg aagtaaacga cacaagttgc tatggaagta
    15781 ttgaagtgga ttggaatggg aggattacag ggttttatga gaaatcggaa agcggtaaaa
    15841 aaggcaaaat aaacggtggt atctatctta tagataaaaa ctttttattc ggttttaatt
    15901 tgccggacaa gttctcaata gagaaggatt tttttgaaaa aatatacaga gatcataata
    15961 tctacgcgat gctattcaaa aactatttta ttgacatcgg cgtaccggaa gaatattcaa
    16021 aaaccaaaga tgactttaga cacttcagat atttttaaag attgggcact tttcctcgac
    16081 cgcgatggcg ttatcaataa gcgaattgta gatgattacg taaaatcatg ggctgatttt
    16141 gttttttgcg aaggagcaat agaagcgtta aaatttttca atactcattt taaaagaatc
    16201 attattgtta ccaatcagca gggcgttggc aaggggctga tgacggaaga agaattgcaa
    16261 cagatacatg aaaaaatgac tacggccatt gatcagtcag gtggtcgcat tgacaggatt
    16321 tacagttgca cccgtttgaa atcagaaaac ccattttgcc gaaaaccaaa ccccggtatg
    16381 gcattggctg cacgtagaga ttttccggat attaatttca aattttcaat tatggtagga
    16441 gactcaataa gtgacctcaa atttgggaaa aagatgggaa tgaaaacagc tcttatagct
    16501 cctgatttta aaattgcacg tcagtttcca aaactaacca acttgtggtt taccagtctt
    16561 atccactttg ccgatttcat tagagagaca aaaaaatatt aacaggcttt atccaattca
    16621 tttattcatt aattatttta tgaaccatca gttgatactt attatactcc ttacctacac
    16681 tttaatgctc tttatcgttg gctggatcac ctcaaaaaat gcggataacg aatcattttt
    16741 tcttaataat cgtaagtcga attggatggt cgttgcctat ggaatgattg gcgcttcact
    16801 ttcgggagta acatttatct ccatccctgg ctgggtgtcg gacacacagt tttcgtatat
    16861 ggtattggta tttggctatt tactcggata tttagtcatt gccaaagttt tattaccgtt
    16921 atattataat cttggcttaa cctccatcta ttcatacctc gatcaaaggt ttgggagatg
    16981 gtcatataaa accggcgctt tttattttct ggtatcacga ttaattggcg cctcttttag
    17041 aatcttcctg gtaataaata ttttacagat ttttgtattt gacaaattag gcattccgtt
    17101 ttggacatca gcagttgcgt ttgttctatt aatcaatctt tacactttta gaggcgggat
    17161 aaaaacaata gtgtggactg atgccttgca aactacattt atgcttgctt ccgtgataat
    17221 caccgccatt tttattaaag acgaactaat gatgaccacc cgaactatgt ttcagtcaat
    17281 tgcaaaaagt gaatattcgc aaatgatctt caccaactgg caagatcagc gtttttatct
    17341 taagcagttt ttctcaggcg cttttattgc tattgttatg acaggtttag atcaggatat
    17401 gatgcaaaaa aacttgagtt gccgctcact taaggatgcc cagaaaaaca ttatcagcct
    17461 aagctgggca ttaatccctg taaatctgct ttttctaagc ttaggaacgt tattgatgat
    17521 ttttgccaat actaaaggaa tagtaatacc cggtacatct gatacaagat ttcctgaaat
    17581 tgcttttaac tatctacctt caatggcagg cattgtcttt gtaatcggat tgatatccgc
    17641 cgcatattcc agcgccgata gtgcaatgac agccctgacc acttcattta cagttgattt
    17701 ccttggaata aaacgaaatc ctaaatacag tgtcgaaaaa caaaaaaaga taaggcaaat
    17761 ttctcatttt ggaatggctc tggcttttat aatcgtcatt atcatgttca aagcgattaa
    17821 caattcagca gttataaacg agctttttac tattgcaagc tatacttacg ggccattact
    17881 tgggctttat gccttcggac tctttaccaa aagacaaacc cgcgatcggt tcaccccatt
    17941 cattgccata cttgcgccat taatctgcta tgtactaagt aaaaactcag aagaatggct
    18001 gtggggatac aaattcagct tcgagctact gatcctaaat ggattattaa tgtttctcgg
    18061 attaatgatc ttttctgaac gaaagcatgt caataaatga tcgagcagat taaagcaata
    18121 ttgatcggaa gaaagcctct ccgagccgaa ctgctgattt catcaaacaa ttaccggtac
    18181 aatggctcca aatgcttgac gtggtttaaa actttaaaaa taccccacct aaaatattaa
    18241 tccctgcttg cggaaaataa ccatcctgat gatattcacc gccatcttcg aaatatcggt
    18301 aaatccaggc atgtgtaatg tattttctat caaagatatt gttaactgta aagctgaggt
    18361 caattccttt tataaaacca gtagagatcg aataacgaaa catcagatca tttacaaaat
    18421 agggatcaag tttacgatcg tcattcgatg tattatcaat atactgtttt cctacatatt
    18481 tagatatcaa agcgacatca aagccttcca aaattctata ggacaaaatg ctgcttcctg
    18541 taaccgaagg tgaaaatgat atatcggtag ttcccaggtc agtctctatc tgtaaaaaag
    18601 gcgggctcca attatcaaca tattcagtaa agttcaggat tttattttta ctaaaagtaa
    18661 ggttagcctg ccagttcaaa tttgaagtag gcttccatcc aaaagccaac tcaacacctg
    18721 cacgataact attaggcacg ttggtaaaca caggatcgcc taccctattg atttttcccg
    18781 tcatcaccaa ctggtctttg taatccatga aaaataaatt agtgtgaaaa cgaaaatcat
    18841 ttgatgtata ggtatgacct gcttcaatat ttaaaagcag ctcttcctta ggttggttat
    18901 ctagatcggc atcttttaaa tcggtccttg tcggctcacg attggcaatg gcagcagaaa
    18961 aataaacctg atttcgctta tttaccggaa caacaaaccc tgccttagga ttgacaaaca
    19021 gataatcatg tttttgcgat atatctaaaa gattatcatg aataccattg attgaataat
    19081 tgatagctcg taattgcatt tcggcaaaca gattaaacct ttgattatgt tcataattaa
    19141 ctttgccaaa aagattgtac tgctttttaa ttccattgtt ttgataccaa agaaaatcct
    19201 cagggatatt ttcaccaaaa tctgccctta cgatttttcc gtaatggttg ccgtcataat
    19261 aattatatcc tccaccaata atgctttgca atgtttggct atgttgatat ttaaacgaaa
    19321 aatttccacc ataaaaagca ttatcaagat attttctccg tactaaatct gttctcttta
    19381 ttgtgtcgtt tccaataata aaatctgata ggccgtaatt tgaaaacttt tgattttgct
    19441 tcatattctc gaaatatccg aaacccttta ccataaaagc agttaaattc ataatcattt
    19501 tacggtttaa cgtcctagaa caagtaaatt ggtaatgatc ctgtttatag ttatcggttt
    19561 cattatcata ataacctatt gatccatctc cattataata aagtcctgac gggttgaatg
    19621 tgcgatttgt aaacaagctg tcgtatggcg ctccattcca agcctgatag gtcttttctc
    19681 tacccgatat catattgaat ttcaccaaag tcttgtcggt aaaataacct gccgagaaat
    19741 agatggactc taagttagat gtcgccctat caacatagcc atcgctattt atttttgaaa
    19801 accttccgtc taaggtaaat tttccgtcaa tcaatcctgt cccgaccgaa attgcatttt
    19861 taaatgtatt aaaactaccc ataatgctac ttatttcagc ataaggatca gccctcagcg
    19921 attgtgtctg gatattgata cttcctccga aagctgctgc tccatttgac gatgttccca
    19981 cgccgcgctg aatttgaata ctttctattg atgatgcaag atcaggtaga ttaacccaat
    20041 agacgctgtg cgattccggg tcattccact gaaccccgtt taatgttaca ttgatccggg
    20101 taatatcagt tcctcttatt tttagccccg tatatcctat tccggcgcct gcgtcggaag
    20161 ttgctaccaa acttggtgta gtagcaagca aaaaaggaat atcattacca aaattacttt
    20221 tctgaatctg ttccttttcg atattagtaa atgttacagg cgaattaccg gctacacgtg
    20281 atgctaaaac aaccacctcc tcctccatta tcggccgtct ggacattcca atgtcaacaa
    20341 attcatcact tttcacctca ataacctgta caacattatc ataccctaaa taggtaactt
    20401 taagagtgta aacgcctggt tttaaatttg taaaaccgaa ctcgccccgc tcattactta
    20461 tttgagtaag aaaagttggt tgtaacgaca aatgcgctcc agaaagcgct tcattggttt
    20521 gagagtcgcg aataacgcca ctcaaactaa tttgagcaaa aacatttaaa gtaaatgcta
    20581 ttgcaaaaca aagtaaaaac aactttttca tcgttcgatt gatttttaag ttcaacatta
    20641 aattgaaata aaaatcaata gaggaattat aagtcaggtt atccattatt ccctccgccg
    20701 atattactcg gatcaggttc aatgggtatg atctcagccc gattgttttg ggcaccccct
    20761 attagatttt agtccggcaa atgtacgaaa aaaataataa ttgaatctaa tttttcaaaa
    20821 atcataaaat tggattgcat tttgcttatg aggcaacttt gacactgaaa gagattttac
    20881 caaactgaaa tgcctttaaa tattttccac tatttactcg tttactatgc gtcaggtttt
    20941 aaaaatgtca ttaaaaaaat atatttttct aataattctc ctttcgggaa ctttcggtta
    21001 tagccaaaat tccgggttaa ttgttctgcc aacaacagtg gtaaatggag acactatccc
    21061 acacatccgg cttcctgaag cggaaatatt tggttacaaa atttttaaaa acaaaagaga
    21121 agagtatcgg tatgatcgtc ttgtaaagaa tgtgagaata gtatatcctt acgcaaaact
    21181 tgccggcgaa atgcttagag aatatgagca gatactggcg gcaataccga gcgagagtga
    21241 aaagagaaaa ataatgaaag accttgaaga cgacctacac aaagagtacg atgacgagct
    21301 taaaaagtta acgatcacgc aaggcaaaat acttatcaaa ctaattgacc gccaaacggg
    21361 agattcgtca tacgacttag ttaaagagtt tcggggagcc tttagggcat ttttctatca
    21421 aaccttcgca cgtatttttg gtttaaatct aaaaatgaaa tacgaccccg aaggagagga
    21481 taaaagtatt gaaacaatag taaggatgat tgaaaaagga gttatttagt tactgttttt
    21541 gaaaatatca tttttatgac aacaaaaaat cattaaacca aaaacatccg tttacaacgc
    21601 catcattaca gatacgccat cttaattgac gcaaaaaata ctactactca caacctttaa
    21661 ggcgacgctc cgaaagcgcc tatgtacaat tattttcttt taaaaataga tttcattaat
    21721 ggctattcta ttgattaagc ctattattgc aaaatggttt tattattata tacttcaaaa
    21781 tgaaaaagat caaagaaaca agagcctatt ttattacagt gccggctcaa gaagaatttg
    21841 agaattaccg tacaagcgcc aaccggcaat tgttattaac aatgatttat gacgaaaatg
    21901 gcaacctcct tgaagatcac aaatatgata attggggaaa attattagga aaaacagttt
    21961 atcgttatga taatcaaaac aatcgaatct cagaggagac tttcgaccca gaagacaatc
    22021 ttgaagagcg cactacattt gagtttgaaa ataacaagcc cgtgaaagga tatatccatt
    22081 atcttgacga ctccaaagat gaaattcttt ttcattacaa ccagtcaggc aaattaatta
    22141 tgaaagaatg gatttcagac gaaggcgaaa ttgataaaac ggaacaattt gtctttagtg
    22201 gcgatcagct tgttttacat aaaataacag aggatgggga aaccatttt
//
LOCUS       DAOOAI010000027        21517 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_3225_length_21517_cov_5.241031, whole genome shotgun sequence.
ACCESSION   DAOOAI010000027 DAOOAI010000000
VERSION     DAOOAI010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 21517)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 21517)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21517
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3225_length_21517_cov_5.241031"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            165..2126
                     /locus_tag="PK345_03280"
     CDS             165..2126
                     /locus_tag="PK345_03280"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPX34075.1"
                     /translation="MNRFLAYLAFVLNILLFMLAKQTFAQPLFFEDNTDLIAKTAMMR
                     YQRMNQPNNSRAGLNIDIVYTRFEWDVDPSVRYISGNVLHHFKAIENLESILLELNDN
                     MVIDSVIFRNRQVECNFVSNHEFVINLEVLVEAGRMDSVRIYYQGEPIQNSGFGSFEI
                     KEHNGVPAMWTLSEPFGAKDWWPGKNDLTDKIDSIDVYIRCPLPYNGVSHGLLQSVIE
                     EGDKKVTHFRHRYPIASYLVAFAVSNYVIFPQQAILGHDTLPIINYVYPEDSAFIAEV
                     SANTPEMIQLFDTLFAPYPFKNELYGHAQFGRNGGGMEHQTVSFMASWAHDIRAHELA
                     HSWFGNMITLATWHDVWINEGFATYANGLSFENMGDGYWWPIWKNVTLNAILSAPDGS
                     VYVADTTSISRIFDSRLSYHKGAYVLHMLRWLMGDQAFFSALRNYLNDPELKYRFATF
                     EDVKTHFEKISETNLDSFFDQWYYGEGYPVYGIDILKIVQTDELLITINQEQTHSSVA
                     FFDIPVEIKLYGGEQEKSFLCRPTFSGEQFRFPAPNFHIDSAKFDPDKWVIAKLSYIS
                     LKVDEPQLDKLTIFPIPAIDKVVLFIPDKRLEHICVFDVTGHEVFSANIDIFNQEYEI
                     VTENFLPGVYFCKVKTDKEVFYARFIISK"
     gene            2336..3082
                     /gene="gpmA"
                     /locus_tag="PK345_03285"
     CDS             2336..3082
                     /gene="gpmA"
                     /locus_tag="PK345_03285"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005857811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HPX34076.1"
                     /translation="MKKLVLLRHGESVWNKENRFTGWTDVDLSPKGVEEAIEAGRVLK
                     ERGFMFDMAFTSYLRRAIKTLWLTLEEMDQMWIPVYKSWRLNEKHYGLLQGLNKIEMA
                     EKYGDEQVLLWRRSFDVPPSPVPEDDKRHPKYDPRYKDLKPEEIPATESLKETIGRMV
                     PYWENDIRPALQKNNQILVAAHGNSLRAVVKYLKNMSEQEIIAFNIPTGIPYVFEFDE
                     NLNLLKDEFIGDPETIKKLMDAVANQAKRK"
     gene            3358..4074
                     /locus_tag="PK345_03290"
     CDS             3358..4074
                     /locus_tag="PK345_03290"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPX34077.1"
                     /translation="MKQWVIPDLHGCLRTLIGMIEDRIRPTKEDELYFLGDYVDRGPD
                     PKGVLDYIMNLEEQGFKIFPLRGNHEEYILLALENERKLKRRFLFFKERNRLYDEWMS
                     AGGASTMKSFGVNKVNQIPEKYVEWIKRLKYYYILENHILVHAGLNFSRRDPFDDLHS
                     ILWASSFTPMPEKVDNKIVIHGHVAVSLEFLQNVLADPSKKYIPLDTGCHHSERSGMG
                     NLVALELNTMELLIQPNIEK"
     gene            complement(4168..5175)
                     /gene="holA"
                     /locus_tag="PK345_03295"
     CDS             complement(4168..5175)
                     /gene="holA"
                     /locus_tag="PK345_03295"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPX34078.1"
                     /translation="MSYEQIIGDINRKIFYPIYFLTGEEPHFIDKISKLIETTVLSED
                     EKEFNQIIVYGRDILPNQIIQMAREYPVFGNYRVIIVREAQDIKQIEKDKLLLAYLEK
                     PVPTTLLVFDYKYKKVDGRTSFVQLLKKNGVFFESKKLYDNQVPKWIEGTIRGMGYQI
                     NPEAMMLLSENLGTDLSKIENELKKLVINIDKNTEITPDIVEKNIGISKDFNIFELQK
                     ALGERNIYKANQIINFFASNPKDNSVVFVIVMLFNYFKRLLIYHTLKDKSDKNVASKF
                     GINPYFVREIRIASSRYNIAKLRSIISILREYDQKAKGVETAPIEDGELMKEMIYKIL
                     H"
     gene            complement(5185..5955)
                     /locus_tag="PK345_03300"
     CDS             complement(5185..5955)
                     /locus_tag="PK345_03300"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004295947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008714 - AMP nucleosidase activity
                     [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA];
                     GO_process: GO:0044209 - AMP salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HPX34079.1"
                     /translation="MKTKKDIVHNWLPRYTGIPLEDFGKYILLVNFNKYLELFAQWNG
                     VEIIGLDRPMPSLTVGDITMIKFGMGSPNAAIIMDLLSAIKPKAALFLGKCGGLKKKN
                     QLGDLILPIAAIRGDGTSNDYFPAEIPALPAFSLQKAISTTIREYGLDYWTGTVYTTN
                     RRVWEHDDKFKKYLRTTRSMAIDMETATIFIAGFANQIPTGALLLVSDQPMISEGVKT
                     EMSDNMVTEKYVETHLNIGIESLKQLIENRQTVKHLRF"
     gene            6024..6479
                     /locus_tag="PK345_03305"
     CDS             6024..6479
                     /locus_tag="PK345_03305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction enzyme HsdR N-terminal
                     domain-containing protein"
                     /protein_id="HPX34080.1"
                     /translation="MNRLNLPAFDYRCRTRDGREEIFDPVRRKYVALTPEEWVRQHFI
                     NFLITEKATPLPLIAVEQMIVYNTMQRRCDIIVYSRKGTPRLIVECKASSIEINQKAF
                     EQIARYNLVLKVPYLVVTNGLTHICCKISFEQSNYVFLKEIPSFDQLTL"
     gene            complement(6602..7792)
                     /locus_tag="PK345_03310"
     CDS             complement(6602..7792)
                     /locus_tag="PK345_03310"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011203214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HPX34081.1"
                     /translation="MKTIDNLNFKGFRAIIRVDFNVPLNDKFEVTDTTRIDAAIPTVK
                     KILNDGGIVILMSHLGRPKSGPEEKYSLKHLLPVLDKKLGLKVKFADDCIGEEAVRIA
                     ASMKSGEVLLLENLRFYKEEEKGDEAFAHKLARLADVYINDAFGTAHRAHASTAVIAK
                     FFPKDKYFGYVMAGELASINKVMKEPSRPFTAILGGAKVSGKIEIINNLLDKVDNLII
                     GGGMMFTFIKAMGGQIGNSLVEDDLIETARKAIIDAKEKGVNMLIPTDSIIADKFDNE
                     ANRKSCQANKIPDGWMGLDIGEQTITAFEKTIKNSKTILWNGPMGVFEMSNFANGTKK
                     IALAIADSTSKGAFSLIGGGDSVAAINKFNLATKVSYVSTGGGAMLEFIEGKTLPGVA
                     AIED"
     gene            7918..9072
                     /locus_tag="PK345_03315"
     CDS             7918..9072
                     /locus_tag="PK345_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine aminotransferase"
                     /protein_id="HPX34082.1"
                     /translation="MIYKGNLNSKLPNSEQSIFATMSKIATEHGAVNLSQGFPDFDIS
                     SDLIDRVNHYMKSGNNQYAPMPGVLELRQAISDKIKRSHGVFYDPETEINITSGATQA
                     LFTAIEAFVREEDEVIIFDPAYDSYAPTVRLNGGHVKHIQLESPGYTINWDHLAKMIS
                     NRTKMIIINTPHNPTGSVLTKKDMEQLQRIIQKSDIIVLSDEVYEHMIFDGLTHYSVC
                     RFPELAQRSLVVGSFGKTFHATGWKIGYIAAPENLMREFRKVHQWVVFAVNTPIQYAI
                     ADFLKDEKNYKELSGFFQRKRDLFLDLIKGSRFKPITCSGTYFQCINYSSITDDDDLE
                     FAKRLTIDHKVASIPLSPFYKNGHDDKILRFCFAKKDETLIQAAKILCTI"
     gene            9060..9860
                     /locus_tag="PK345_03320"
     CDS             9060..9860
                     /locus_tag="PK345_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPX34083.1"
                     /translation="MHNLKVALVQSSIVWEDVAANLMNFDKKLEAIAPTTDLVLLPEM
                     FNTGFSINPLKIAEKPDGVTFVWLKEKANYLNCVVAGSVLTVEAGKYYNRLYWMRPDG
                     TYEIYNKRHLFRLGEEFKVFTPGKERKIVELKGWKILPIVCYDLRFPVWIKNRYNNGQ
                     YEYDLIVCVSSWPAARQYAWRSLLIARAIENQACMAGVNRVGIDGEGKPHTGYSVIAD
                     ALGSVIAQAEPDTEQIITATLNWDELQNFRKQFTIGLDWDEFEIKIDM"
     gene            9909..11249
                     /gene="trkA"
                     /locus_tag="PK345_03325"
     CDS             9909..11249
                     /gene="trkA"
                     /locus_tag="PK345_03325"
                     /inference="COORDINATES: protein motif:HMM:NF007031.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HPX34084.1"
                     /translation="MKIIVAGDGEVGFHLAKLLTDELHDITVVDPHSELLKMVESNTD
                     LMTITGDSTSISILDRANVKRADLLISVLHDENVNLMTCIIGKKLGARKTIARINNTE
                     FIDHGNEALFNSLGIDHLVCPERLAAEEIVDLLDRSAATETFEFSKGKLLLFLIKLDE
                     KALVIGKTLDQIASEFSYLDFRAVAIHRNSRTIIPRGNDQFMINDLTYVVTKPDGIKT
                     LLKLGGKEEFEIKNVMIAGGGRIGLKTSRRLENSHKIKLIEIDREKSVSLSEELDNVL
                     IINGDARDIDLLQSENVEDMDAFIAVTDSSETNILTCLHARKFGVKKTIALVENIDYI
                     DISQNIGIDTIINKKLITASYIARFTMQSEIATNKVLHGIDAEVFEFVVKEDSLVTKK
                     SIENLNFPDGAIIGGITRGNESYIAVGNFKIKAGDRVIVFSLPSTIHKVDRFFR"
     gene            11253..12752
                     /locus_tag="PK345_03330"
     CDS             11253..12752
                     /locus_tag="PK345_03330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter TrkG"
                     /protein_id="HPX34085.1"
                     /translation="MATKKYINIRAILQILGLLLIIEAFFMLFCIPFSWYYGSTDFFD
                     WNIEDANHDFWPLVISAAITFISGIILYFGSRKAIREDIGKREGYIIVSIAWVVISLF
                     GALPYILSGSIPNFTNAFFETISGFTTTGSTILNDIEALPKGILFWRAITHWIGGMGI
                     IVLSLAILPFLGIGGMQMYIAEVPGPTHDKLHPRITQTAKLLWGIYVLLTFIQTLLLM
                     LGGMDLFESLAHSFATMATGGFSTRNDSIASFSPYIQYVITFFMFLAGMNFTLHYMAL
                     HGRIREVLRNEEFRYYFFLVIISTLIITIVLTITGTYGLEASFRNAIFTVLSIMGTSG
                     FVTSDYMMWPGALWFIIFMLMFVGGSAGSTGGGIKVVRHMLLLKNSLLELKRLLHPQA
                     VIPVRMNGRPVSQEIIFKVLAFFLMYIIVFAAGVFALSICGLDFETSVGASISSIGNI
                     GPGIGKVGPAYNFTVIPDPGKWVLMILMILGRLEFFTVLILFSPAFWKR"
     gene            12819..14150
                     /locus_tag="PK345_03335"
     CDS             12819..14150
                     /locus_tag="PK345_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HPX34086.1"
                     /translation="MNSIPSYRNIWNISYPIILSLLAQNIITVIDTAFLGRVGEVELG
                     ASAIGGLFYFSFFMLGFGFGNGAQILMARRNGEKNFPMVGKIFDHTLYIFAIMSALLI
                     LFTFLISNQLLKWFISSEAIYHASVTYFNIRVLGMFFSFANVAFRAYLVGITKTKLLS
                     YGAAIMAVVNVALDYLLIFGHYGFPEMGIAGAAIASLISEATAIAFYTIAILVKHENR
                     KYELFKFPRFNKEIVATTWSISIYIMIQNFISMVSWFLFFLFIEHLGERSLAISNIIR
                     SIYMTLMIHVWAFSSSVNSLVSNSIGSGLHDMVLPIIRKVNRLSILITLLVIFITVTI
                     PGLLIRIYTDDITLINEAIPVLFVVVGAVLPVALANNWFSGVSGTAKTNVALVIEIFC
                     IIFYLTYVWLVIFKFDVSLPVVWISEYVYIFLLGAISYLYLKFGKWQTKII"
     gene            14165..14983
                     /locus_tag="PK345_03340"
     CDS             14165..14983
                     /locus_tag="PK345_03340"
                     /inference="COORDINATES: protein motif:HMM:TIGR01533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'-nucleotidase, lipoprotein e(P4) family"
                     /protein_id="HPX34087.1"
                     /translation="MRRVSYSLILIVILILVSSCRVGIKTTTDINYQQQLLAATLFQQ
                     CAAEMKALSYQAYNIAGMRLEEALKEANDPHHLAVILDLDETVLDNSPHSGQSIIDRF
                     SFPIGWDEWCYKAEAKALPGAVDFLKKADKAGVQIFYITNREEKHREATKENLLKLEV
                     PVQSDNHLMFKTVTSSKRERQMKVSENYEIIMLIGDNLTDFTPLYDKQSVEQRDKLTD
                     SLKIEFGNRFIVLPNTMYGDWMSAIHNFNFSLSDSLKTVIFNDRIKGFNFHPNE"
     gene            14976..15692
                     /locus_tag="PK345_03345"
     CDS             14976..15692
                     /locus_tag="PK345_03345"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: protein motif:HMM:NF001453.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPX34088.1"
                     /translation="MNKYWFILVFLLFTCNCNSQTHTAPQQIKERNELVRVYIEGQGV
                     KDKRVIAAMKKVERHRFVPNNLLPYAYYDQPLPIGEGQTISQPSLVAFMTELLELKQS
                     DKVLEIGTGSGYQAAILGELVDSVFTIEIIKPLGVKAEKLINNLGYSNIFVKIDDGYK
                     GWPEHAPYDAIIVTCAPSDIPNPLQQQLAENGRMVIPVGKDGSVQKLILLRKRKGKII
                     RETVLPVRFVPMLKEDGGKY"
     gene            complement(15689..16747)
                     /gene="mtnA"
                     /locus_tag="PK345_03350"
     CDS             complement(15689..16747)
                     /gene="mtnA"
                     /locus_tag="PK345_03350"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HPX34089.1"
                     /translation="MKVNGKEYRTIWMEGASVMMIDQNRLPFDFFVFRSENFLTTCDS
                     IRNMTTRGAGAIGAAAGFAMAQAFLAAPVKNRDKFLLQAKETIINTRPTARNLFYAVD
                     LVYEAGLISPKAAYEQAQQIAEKDIADSRAIGKFGSMLINNGDHILTHCNAGWLAFVD
                     YGTALAPIYRSVEDGKNVFVWVDETRPRGQGARLTAWELHNAGIDHRIVPDNAGAWLM
                     SNKRVDLLITGADRIAANGDTANKIGTYEKAIVAKHFGIPLFIAAPTSTIDLTCTTGN
                     DIVIEERSEDEVLYQSGPDESGKINRIRVASPGSQAINPAFDVTPAKFITGIITEKGI
                     IKPKKEEITKLFKGRINS"
     gene            complement(16761..17288)
                     /locus_tag="PK345_03355"
     CDS             complement(16761..17288)
                     /locus_tag="PK345_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010993275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPX34090.1"
                     /translation="MTVRNYKELLKKITTMIFDYDGVFTDGKIILTEAGEQLRTANVK
                     DGYALQLAVKKGYRIAVISGGTSDAIVTRLHNLKINDVFIKVDHKIKVYKEYLKKHNL
                     IPEEVLFMGDDIPDLQIMKEAGVVICPADAAEEVKALSHHISHLKGGEGCVRDIIEQV
                     LKVQGNWLDDDAFIW"
     gene            complement(17269..18078)
                     /locus_tag="PK345_03360"
     CDS             complement(17269..18078)
                     /locus_tag="PK345_03360"
                     /inference="COORDINATES: protein motif:HMM:NF022188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2520 domain-containing protein"
                     /protein_id="HPX34091.1"
                     /translation="MAISTVVFIGAGRIATQLGLSLIKESISVLQVYSRTIESASALA
                     SLLKCNFTNDITSLTKKADLYIISISDNFIAEIAGQLHLQNKIVVHTSGSVPIEILKN
                     TSNHFGVFYPLNTFIKEKIINLSTTPFCIEASDDQTEEKLIGLASRITSDVRQINSKE
                     RAVIHLAAVFACNFTNFMMVNADEILKANGISFDILMPLIGETIEKLYDISPKMAQTG
                     PAIRNDTKVMQKHLEMLKNDPNKSMIYQLLSSQISNFFETDKNKADDSKEL"
     gene            complement(18085..18741)
                     /gene="rpe"
                     /locus_tag="PK345_03365"
     CDS             complement(18085..18741)
                     /gene="rpe"
                     /locus_tag="PK345_03365"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004370381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HPX34092.1"
                     /translation="MAHLIAPSLLSADFSNLKAELDMLNQSDADWIHVDVMDGKFVPN
                     ISFGQPIIRQIRQLTTKPLDVHLMIVEPERYIQDFKNCGADLLSIHYEASIHLHRNIK
                     HIKTLGMKAGVVLNPHTNVMLLDDIIAEVDFVLLMSVNPGFGGQKFIENTFQKISKLK
                     ELILKKSGKALIEVDGGVTLQNAPSLIKHGADVLVAGNTIFSSSNPAKTISELKSLII
                     "
     gene            complement(19307..>21517)
                     /gene="pnp"
                     /locus_tag="PK345_03370"
     CDS             complement(19307..>21517)
                     /gene="pnp"
                     /locus_tag="PK345_03370"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HPX34093.1"
                     /translation="REISIETGRLARQADGAVIVKQGKTMLLATVVSSKEVNEDVDFL
                     PLTVEYQEKYAATGRFPGGFFKREARPSEYEILISRLVDRALRPLFPDDYHAETQVLI
                     TLISGDKNDAPDALAALAASAALMVSDIPFNGPISEVRVARIEGQLIINPPIDKMEAA
                     DLDVIVAATTKDIIMVEGEMKEISEAEMLKVLKFAHDHIKIQCQAQHELAGMIEKAAN
                     KREYSHETNDEELKERMYTELYPKCYEVAGLGTAKQQRSELFNKILEDFVDTFQEEEA
                     VEKASMIKRYYHNIEKEAVRNFVLDERKRIDGRKLDEIRPIWSEVDYLPSVHGSAIFT
                     RGETQALVTVTLGTKLDSQVIDGAVFEGKSDFILHYNFPSFSTGEVRFRGGTSRREIG
                     HGNLALRALKPVIPDSSINPYTIRVVSDILESNGSSSMATVCGGTLALMDAGVKIEKP
                     VSGIAMGLITNKDGSKYAVLSDILGDEDHLGDMDFKVTGTRDGITACQMDIKVDGLSY
                     EILEEALAQALKGRLHILNEIEKTISTPREDYKPHVPRIEQMTVPKEFIGAIIGPGGK
                     IIQEIQATTNSTIVIEEVGEYGIVDIVSVDKESIEAAKEKIKAIIAIPEIGNTYRGKV
                     KSIITFGAIVEILPGKEGLLHISEIDWRRIANVEDVLKVGDEVDVKLLEIDSKNGKLK
                     LSRKQLLPKPEGYQESRRREKGDKPQRDYRNNRDHRPKGDSDNRSFRPDRPPRS"
BASE COUNT         6231 a         3929 c         4256 g         7101 t
ORIGIN      
        1 aataagtttt agtaatactt tcacacaaaa aaaatttatg caaagatgcg taaaatgact
       61 ttgctatcaa tgttttttct aattttggcg atacaatcat caaaattgat ataaattttg
      121 aagatggatt tcaaccaaca ctttaattca aaaatgatta taagatgaac cgatttctgg
      181 cctatttagc atttgttctc aatattttat tgtttatgct tgcaaagcaa acttttgcac
      241 agcctttgtt ttttgaagat aataccgatc ttattgctaa aacggcgatg atgaggtatc
      301 aacgtatgaa tcagccgaat aattcacgtg ccggattaaa tattgatatt gtttatactc
      361 gatttgagtg ggatgttgac ccttcggttc gctatatttc tggaaatgtt ttgcatcact
      421 tcaaagcgat tgaaaactta gaatctattt tactcgagct gaacgataat atggttattg
      481 attctgtaat ttttagaaat cgtcaggttg agtgtaattt tgtttctaac catgagtttg
      541 ttattaatct tgaggttttg gttgaagcag ggaggatgga ttcagtgcgg atttattatc
      601 agggcgaacc aatacaaaat agcgggtttg gctcttttga aattaaagaa cataatggag
      661 tacctgctat gtggacactt tctgagcctt ttggtgctaa agattggtgg ccaggtaaaa
      721 atgacttaac tgataaaatt gattcgattg atgtttacat cagatgtcca ttaccatata
      781 atggtgtcag tcatggactt ttgcaatcgg ttatcgaaga gggcgataaa aaagttacac
      841 atttccgcca tcgctatccg atagcgagct atctcgtggc ttttgcggtt tccaattatg
      901 taatattccc tcaacaggcg atcttaggac acgacaccct tccaataata aattatgtct
      961 atcccgaaga ctcggctttt attgctgaag tttcagccaa cactcccgaa atgattcagt
     1021 tgtttgacac tctctttgcc ccgtaccctt ttaaaaatga attatatggt catgcgcagt
     1081 ttggtagaaa tggtggaggt atggagcatc agacggtgag ttttatggca agctgggcgc
     1141 atgatatacg cgctcatgag ctggcacatt catggtttgg gaatatgata acgcttgcca
     1201 catggcacga tgtatggata aatgaaggct tcgcaaccta cgccaacggg ctttcttttg
     1261 aaaatatggg cgatggctat tggtggccta tctggaaaaa tgtaacactt aatgcaatcc
     1321 tttctgctcc cgacgggtct gtctatgtgg cagacacaac ctctatatct cgtattttcg
     1381 attctcgtct gtcgtaccac aaaggcgctt atgtactgca catgctgcgt tggctgatgg
     1441 gcgaccaggc tttcttttct gcattgcgca attatctgaa cgatcctgag ctaaaataca
     1501 gatttgcaac ctttgaagat gtaaaaactc actttgaaaa aataagcgag accaacctcg
     1561 attccttttt tgatcaatgg tactatggcg aaggctatcc tgtgtacggc attgatatcc
     1621 taaaaattgt gcaaaccgat gaattgctta taaccataaa tcaagaacaa acacattcct
     1681 ctgtagcatt ttttgatata ccggtggaga taaaattata tggtggcgag caggaaaaaa
     1741 gctttttgtg ccgcccaacc ttttcgggag aacagtttcg gtttcctgca ccaaattttc
     1801 atattgattc cgccaaattt gatcctgata aatgggtgat agcaaagctt agttatattt
     1861 ctctaaaggt tgatgagcca caactcgata aactcactat atttccgata cctgcgatag
     1921 ataaagttgt tttgtttata cccgacaaac gtttggagca tatctgtgtg ttcgatgtaa
     1981 ccggccacga agtgtttagt gctaatattg atattttcaa tcaagaatat gaaattgtta
     2041 ccgaaaactt tttgccgggc gtttattttt gtaaagtaaa aactgataaa gaggtctttt
     2101 atgcacggtt tatcatatca aaataggctg aaagacaatt ttattaaatg ttagataccc
     2161 ttttaacgtt tcacttagtt agtgccaata aaactctatt tggaattagg ttctaatcag
     2221 aattagaaat tgaaagttta tgttagttct gttcaattca atatttatag tgtatttggc
     2281 gttatcatct atatttttaa aacaatttat tattaattaa aggataaaaa aaaacatgaa
     2341 aaaactggtt ttgttaaggc atggcgaaag cgtttggaat aaggaaaatc gttttacagg
     2401 ctggactgac gtagatttgt cgccaaaagg tgttgaagag gctattgaag ccgggcgggt
     2461 attaaaagag aggggcttta tgttcgacat ggcttttacc tcatatctcc gcagggcaat
     2521 taaaacgcta tggctgacac ttgaagagat ggatcagatg tggatacctg tttataaaag
     2581 ctggcggtta aatgagaaac attacgggct acttcagggt ttgaataaaa tcgaaatggc
     2641 cgaaaagtac ggtgatgaac aggtattgct ttggagacgt agttttgacg tgccaccttc
     2701 acctgtcccc gaagatgata aaagacatcc aaaatatgat ccccgttata aagatttaaa
     2761 acccgaagag attcctgcta ctgaatcgtt gaaagaaaca ataggtagaa tggtgccata
     2821 ttgggaaaat gacatccgtc ccgcgctgca aaaaaacaac cagatactgg tggcagcaca
     2881 cggtaacagc ttgagagcag tggttaagta tttaaaaaat atgtcggaac aagagatcat
     2941 tgcatttaat attccaacag gaattcctta tgtctttgag tttgatgaga atcttaacct
     3001 gcttaaagat gaatttatcg gcgatccgga gacaattaaa aaactaatgg atgctgttgc
     3061 aaatcaggct aaaagaaaat aataatctct tttattcaaa actttaaacc tgaattaatc
     3121 aataataaac ctctttaggc tgattttatt gaaattaaat aagtaatccc gacatatagt
     3181 gtttgtttaa tgcaaattct cattttgccc attataaaca cttagtttta atcaaaagat
     3241 acattcaatc cctgctgaaa atatataaat gattttttca actttgtgaa atgcagagta
     3301 agcttattta ggtttgtatc ttttctatct ttatttgaag cgcaaaaatg atgagttatg
     3361 aaacaatggg taatccctga tctgcatggt tgcttgagaa cattgatcgg gatgattgaa
     3421 gatcgaattc gcccaacaaa ggaggatgaa ctttatttcc tcggcgacta tgttgatcgc
     3481 ggtcccgatc caaaaggtgt gctggattat attatgaatt tggaggagca gggctttaag
     3541 atttttccac ttcgaggaaa ccatgaagag tatattttgc ttgctttgga aaatgaacgg
     3601 aaacttaaaa ggcgttttct tttttttaaa gaaagaaaca gactttacga tgaatggatg
     3661 agtgccggag gtgcctcaac gatgaagagc tttggcgtta acaaggtgaa tcagattccg
     3721 gaaaagtacg tggagtggat aaaaagattg aaatattatt acattttgga aaatcatatc
     3781 cttgtacatg ctggattgaa ctttagtcgc cgtgatcctt ttgatgattt gcactcaatc
     3841 ctttgggcga gttcttttac cccaatgcct gaaaaagttg ataataaaat agttattcat
     3901 ggccatgtgg cagttagtct cgagttttta caaaatgtgc ttgccgatcc ttcaaaaaaa
     3961 tatatccctt tagatacagg atgccatcat tccgagagat caggaatggg caatctcgta
     4021 gcgcttgagt tgaacacgat ggagctactc atccagccaa atattgagaa atgataatcc
     4081 cgagttaatc aaatagaaag gcttttgtga tgggcttatt ttagtgaaga ctacattttc
     4141 aaatacttaa tctggagtta tgcctgatta atgtaatatc ttgtaaatca tttctttcat
     4201 caattcgcca tcctctattg gcgcggtttc gacacctttt gccttttggt catattcccg
     4261 caatatagat atgatagaac gaagctttgc aatattgtat ctggacgaag ctatccttat
     4321 ctctctgaca aagtaaggat taataccaaa ctttgaagct acatttttat ctgatttatc
     4381 tttaagtgtg tgataaatca aaagtctttt aaaatagttg aataacatca ctattacgaa
     4441 aaccacagaa ttgtctttgg ggttggacgc aaagaaatta atgatctggt ttgccttata
     4501 aatattacgt tcgccaagcg ctttttgaag ctcaaaaata ttgaaatcct tactgatacc
     4561 aatatttttt tctacaatgt cgggggtaat ttcggtgttt ttgtcaatat tgatgacaag
     4621 tttttttaat tcgttttcaa ttttcgagag gtctgttcca agattttcgc taaggagcat
     4681 catggcttcc ggattgattt gatatcccat acctctgatc gtgccttcta tccatttagg
     4741 aacctgattg tcatacagtt tttttgattc gaaaaagacg ccgttttttt taagaagctg
     4801 cacgaaagat gttcgcccgt caactttttt atatttgtag tcaaacacca acaaagtcgt
     4861 tgggacaggt ttttcaagat aggcgagcag tagtttatct ttttctatct gcttgatgtc
     4921 ttgcgcttcg cgaacgataa taactcgata gttgccaaaa accggatact ctcttgccat
     4981 ttgaattatt tggtttggca aaatatctct gccataaacg ataatttggt taaactcttt
     5041 ctcatcttcc gataacacag tagtttcgat gagttttgag attttatcaa taaaatgtgg
     5101 ctcttctcct gttaagaaat atattggata aaatattttt ctattgatgt cgccgattat
     5161 ttgctcgtag ctcataaaag aggtttaaaa tctcaggtgt ttaactgtct ggcggttttc
     5221 gatcagttgt ttcagggatt ctattccgat attaagatgt gtttcaacat atttttccgt
     5281 taccatatta tcactcattt cagtttttac gccttcggag atcattggct ggtcagatac
     5341 taatagcaag gcgcctgttg gtatctgatt ggcaaatcct gcaataaata tggtggctgt
     5401 ctccatatca atagccattg agcgggttgt gcgcagatat tttttaaatt tatcatcatg
     5461 ttcccaaact ctgcggtttg ttgtgtacac cgtacctgtc cagtagtcca atccgtattc
     5521 gcgtattgtt gtcgaaattg ctttttgcag gctaaatgcc ggtaatgccg gaatttcggc
     5581 cggaaagtag tcgttggagg tgccgtcgcc acggattgct gcaataggaa ggatcagatc
     5641 gcccaactga tttttctttt tcaaacctcc acatttacct aaaaaaagag cagctttagg
     5701 cttgatagcg ctgagtagat ccataattat tgcagcatta ggacttccca tgccaaattt
     5761 tatcatcgta atatccccga ctgtcaaact gggcatcgga cgatcaagcc ctattatttc
     5821 aacaccgttc cactgggcaa acagttcaag gtatttattg aagtttacca acaaaatata
     5881 ttttccgaaa tcctcaagag gtatgccggt ataacgtggt aaccagttat gtactatatc
     5941 tttttttgtt ttcatcgaaa aaatattttc tgcaaaaata gttgaaatgg ctgaatgact
     6001 aactttgatg ccagattaat aaaatgaaca gacttaattt accggctttt gattatcgtt
     6061 gtcgcacaag agatgggaga gaggagattt ttgatccggt gcgtcgaaaa tatgttgcac
     6121 taactccaga ggagtgggtg aggcaacatt ttattaattt tttgataacc gaaaaagcaa
     6181 cccctctacc attgatagct gtcgagcaaa tgattgttta caatactatg caaagacgat
     6241 gcgacattat tgtttattct cgaaagggta ctccaagatt aatagtagaa tgcaaagcct
     6301 cttcaataga aattaatcaa aaagcttttg aacagatagc tagatataat ctcgttctga
     6361 aggttccata tcttgttgta accaatggct tgactcatat atgctgtaaa attagctttg
     6421 aacagtcaaa ctatgttttt cttaaagaaa taccctcttt tgatcagtta acattgtaat
     6481 aattctgaac taatcaatag aaatagctat aggctaaatt attgactttc aaaagccaat
     6541 actgtttagc atttattcag tgtaatcttt attgtggtga ttacagatac ttgatcaggc
     6601 tttaatcttc tattgcagca actcctggta atgttttgcc ttcgatgaat tcgagcattg
     6661 ctcccccgcc tgttgataca tagctaactt ttgtggcgag gttgaattta ttgatagctg
     6721 caacagaatc gccgccacca attagtgaaa acgccccttt ggatgttgaa tcagctattg
     6781 caagagcaat ttttttggtt ccatttgcga aatttgacat ctcaaatact cccattggac
     6841 cgttccaaag tattgttttg ctatttttta tagttttttc aaaagcagtt atcgtttgct
     6901 cgccaatatc aagtcccatc catccatcag gaattttatt ggcttggcag cttttgcggt
     6961 tggcttcatt gtcaaactta tctgcaatta ttgaatcagt aggtatcagc atgtttaccc
     7021 ctttttcttt ggcatcaata attgcttttc ttgctgtttc aataaggtca tcttccacaa
     7081 gcgaattacc aatttgcccg cccattgctt tgataaaggt aaacatcatt ccgccgccaa
     7141 tgataaggtt atctacctta tcaagtaagt tgttgataat ttcaatttta cctgatactt
     7201 ttgctccgcc aagaatagct gtaaaaggtc tggaaggctc tttcatcact ttattaatgc
     7261 ttgcaagctc acctgccata acatatccaa aatacttgtc cttcggaaag aattttgcaa
     7321 taactgctgt ggatgcatgt gctcgatgtg cagtaccaaa agcatcgttt atgtaaacat
     7381 cggcaagacg tgcaagttta tgtgcgaaag cttcatctcc tttttcctct tctttataga
     7441 atctaagatt ttcgagcaga agcacctccc cgcttttcat ggatgcagct atcctaacgg
     7501 cttcttcacc aatgcaatcg tcagcaaact taacttttag gcctaatttt ttatccaaca
     7561 ctggtaataa gtgttttaaa gaatattttt cttccggacc agattttggt cttccaagat
     7621 gcgacataag gatcactata ccgccgtcat tgaggatctt tttaacagtt gggatggcag
     7681 cgtcaatacg agtagtatct gtaacttcaa atttatcatt caatggtaca ttaaaatcaa
     7741 ccctaatgat agccctgaat cctttaaaat ttagattatc aattgttttc ataaaattat
     7801 tgttaataaa tttctgtgca aatataacta atagattgat cagtgttttg attttcggag
     7861 ttgtttttgt aaaaagtgtt gagtaaaaaa tattaagttt gccaaaaatt ttaatgaatg
     7921 atttacaagg ggaatttaaa cagtaaacta cctaatagtg agcagtcaat ttttgctaca
     7981 atgtcgaaaa ttgcgacaga acatggcgct gttaatctct cacaaggctt tcctgatttt
     8041 gatatttcat cagatctaat tgatcgcgta aatcactaca tgaaatctgg aaacaatcaa
     8101 tatgccccga tgcctggcgt attggagttg aggcaggcca tttcggataa gataaaaagg
     8161 agtcatggtg ttttttatga tcccgaaact gagataaaca tcacctccgg cgctacgcaa
     8221 gcactattta cagcaataga agcatttgtg agagaagagg atgaggtgat aatttttgat
     8281 cctgcttatg attcctatgc gccaacggta aggttaaatg gaggtcatgt aaaacacata
     8341 cagttggaga gccccggtta cactattaac tgggatcatt tagcgaaaat gatcagtaac
     8401 cggactaaga tgatcattat aaacacgcca cataacccaa caggctctgt tttgacaaaa
     8461 aaagacatgg aacaacttca aaggattatt cagaaatcgg atatcattgt gctgagcgat
     8521 gaagtgtacg agcatatgat ctttgatggg ttaacacatt atagcgtatg ccgttttccg
     8581 gaattggctc aacgcagtct tgtggtggga tcatttggaa agacatttca tgctaccgga
     8641 tggaaaatag gctatattgc ggctcccgaa aacctgatgc gcgagttcag gaaggtacat
     8701 caatgggtag tattcgcagt taacaccccg attcaatacg caattgctga ctttttaaaa
     8761 gatgagaaaa actacaagga gctatctggt ttttttcaaa gaaaaagaga tctgtttctt
     8821 gatttaataa aaggttcaag gtttaaacca attacatgca gcggcactta tttccagtgc
     8881 atcaattata gctccataac agacgatgat gatctggaat ttgcaaaacg acttaccatt
     8941 gatcataagg tggcctcaat accactctcc ccattttata aaaatggcca tgacgataaa
     9001 attttgcgtt tttgctttgc caaaaaagat gaaacattaa ttcaagctgc taaaatctta
     9061 tgcacaatct gaaagtagcg ttagtacagt cttctattgt ttgggaggat gttgctgcta
     9121 acctgatgaa ttttgataaa aaactcgaag caattgctcc gactaccgat ttggttttat
     9181 tgcccgagat gttcaatacg ggtttttcca ttaatcctct caagattgca gaaaagcctg
     9241 atggcgttac gttcgtatgg ctaaaggaaa aagcaaacta cttaaattgt gtcgtagctg
     9301 gaagtgtgct tactgtggag gccggaaaat attataaccg tctttattgg atgcgccctg
     9361 atggtactta tgagatttat aataaaaggc atctattcag gcttggagaa gagtttaaag
     9421 tttttacccc tggaaaagaa agaaaaattg ttgagttaaa aggttggaaa atactaccta
     9481 ttgtatgtta tgacctccgt tttcccgttt ggataaaaaa tcgttataat aacggccaat
     9541 acgaatatga cttgattgta tgtgtatcca gttggcctgc tgcgcgtcaa tatgcgtgga
     9601 gaagtctttt aatagcccgc gcaattgaaa atcaggcttg tatggctgga gtaaatcgtg
     9661 ttggcataga cggagaagga aaaccgcaca cgggatattc cgttatcgcc gatgctttag
     9721 gaagtgtaat agctcaagcc gaacccgaca ccgaacaaat tataacagct actcttaatt
     9781 gggatgagct ccagaatttt cgcaagcaat tcacaatcgg attagattgg gatgaatttg
     9841 aaataaaaat agatatgtag aatttctaca gaattatcta ctttcgcccc caatttttat
     9901 ttacgacaat gaaaattatt gttgcaggcg acggtgaagt tgggtttcac ttagccaaat
     9961 tgcttaccga tgagcttcat gatatcactg ttgttgaccc acacagcgaa ttacttaaaa
    10021 tggtggaatc gaacactgat ctgatgacta tcacaggcga ttctacatct atctcaatac
    10081 tcgacagggc aaacgtaaaa cgtgccgact tgttgatttc ggttttacac gacgaaaatg
    10141 taaatctgat gacatgtatc attggtaaaa aacttggcgc acgtaaaact attgcacgga
    10201 taaacaatac cgaatttatt gatcacggta atgaagctct tttcaattcg cttggcatag
    10261 atcacttggt atgtcctgaa aggctggctg cagaggaaat tgtggatcta cttgatagat
    10321 cagctgcaac cgaaacattc gaattttcaa aagggaaact gttgttgttt cttattaaac
    10381 ttgatgaaaa agcccttgtt ataggcaaaa cattggatca gatagcaagt gagttttctt
    10441 atttagattt cagagctgtt gctatacatc gtaactcgcg aacgataata ccaagaggga
    10501 acgatcagtt tatgataaac gatctcactt acgttgttac caaaccggat ggaataaaaa
    10561 cactattaaa acttggcggt aaagaagaat ttgaaataaa gaatgtaatg atcgccggcg
    10621 gaggccgtat tggattgaaa accagccgaa gacttgaaaa ttcacacaaa ataaaactga
    10681 ttgaaattga tcgggaaaag tcggtaagcc taagcgaaga acttgataat gtattaataa
    10741 taaatggtga tgcacgggat atagacctgc tgcaaagtga gaatgttgaa gatatggatg
    10801 catttatagc agttaccgat agctcggaaa ccaatatact cacctgtttg catgctcgca
    10861 aatttggcgt aaaaaaaaca attgccctcg ttgaaaatat tgattatatt gatatttcac
    10921 aaaatattgg cattgatacc ataattaaca aaaagttgat tacggcaagt tatattgcgc
    10981 gttttaccat gcagtccgaa attgctacca ataaagtttt acatggtata gatgcagaag
    11041 tttttgagtt tgtggtgaaa gaagattcgt tagttactaa aaaatctatc gaaaatctta
    11101 acttccccga tggtgcaatt attggcggta taacacgtgg caatgagagt tatattgctg
    11161 taggtaattt caaaattaaa gcaggtgata gggtaatagt tttctcactt ccatcaacga
    11221 ttcataaagt tgatcggttt ttcaggtaat caatggccac aaaaaagtat atcaacatta
    11281 gggcaatcct tcagatctta ggattacttt taatcataga ggcatttttt atgctttttt
    11341 gtattccttt ttcttggtat tacggaagca cagatttttt tgactggaat attgaagatg
    11401 ccaaccatga tttttggcca ttggtgattt cggcggcaat aacttttata tctggtatca
    11461 ttctttattt tggaagtagg aaggcaatcc gtgaagatat tggtaaacgc gaaggctata
    11521 ttattgtctc cattgcatgg gtagtaattt cattatttgg agctctaccg tatattctta
    11581 gtgggtctat ccctaatttt actaatgctt tttttgaaac tatttccgga tttactacta
    11641 ccggttctac aattttaaac gatatagagg cgcttcctaa aggaattctt ttttggcgtg
    11701 caattactca ttggattggc ggtatgggta taatcgttct ctctttggct attctaccat
    11761 ttctcggtat tggaggtatg cagatgtata tcgctgaagt gcccggccca acacacgata
    11821 aattacaccc tcggattaca caaacagcca aactactttg gggtatttat gtgcttctca
    11881 cctttattca aactttgttg cttatgcttg ggggcatgga cctttttgaa agtcttgctc
    11941 attctttcgc tacaatggct actggtggat tttctactag aaacgatagc attgcaagtt
    12001 tttcgccata tatacagtat gtaattacct ttttcatgtt tttggctggg atgaacttta
    12061 ctctccatta tatggctttg cacggacgga ttagggaggt tcttagaaat gaggagttta
    12121 gatattattt ctttttagtt attatttcaa ctttaattat aactatagtc ctaactatta
    12181 caggtactta tggtttagag gcctcttttc gcaatgcgat ttttactgtg ctatctatca
    12241 tgggaacaag tggttttgtt acgagtgatt atatgatgtg gccaggggct ttatggttta
    12301 ttatttttat gctaatgttt gtaggtggca gcgccggttc taccggcggt gggataaagg
    12361 ttgtaaggca tatgctttta cttaaaaaca gcttgcttga gcttaaacgt cttttacatc
    12421 cgcaagcagt gattcctgta cgaatgaacg gccgtcctgt atcgcaagag atcatcttca
    12481 aagtcctggc tttctttcta atgtatatca tagtatttgc cgccggcgtt tttgctttgt
    12541 ctatctgtgg tctggatttt gaaacctctg tgggagctag tatatcatct ataggaaata
    12601 tcggaccagg aattggtaaa gttggacctg cgtataattt cacagtgatt cccgatccgg
    12661 gcaaatgggt tctgatgatt ctgatgatat taggacgatt agaatttttt actgtattga
    12721 tactcttctc gccggcattt tggaagaggt aaagcgcaag ataggttaaa tccctttttt
    12781 gtaactgaat tgattatttt gtcgcctaat aagctgaatt gaattctatt ccttcatatc
    12841 gaaatatttg gaacatatct tacccaatca tactcagtct tttagcccaa aacataatta
    12901 cggttattga cacagctttt cttggtaggg taggtgaggt agagctgggt gcatcagcaa
    12961 taggcggcct gttctatttt tcttttttta tgttgggttt tggttttgga aatggagcgc
    13021 aaattctgat ggcaagacgt aatggggaaa agaattttcc aatggttggg aaaatttttg
    13081 accatacact ttacatcttt gcgattatga gcgccttgct tatccttttt accttcctta
    13141 tttccaatca attattaaaa tggtttatct cctcggaagc tatatatcac gcatcggtta
    13201 cttactttaa cataagggtt ttggggatgt ttttttcttt tgccaatgtg gctttcaggg
    13261 cttatctggt tggaataaca aaaaccaaat tgctgagcta tggcgctgcc atcatggccg
    13321 ttgtaaatgt tgctcttgat tatttgttga ttttcgggca ttatggtttc cccgaaatgg
    13381 gtattgctgg cgcagccatt gcctcgctga tttctgaagc gacagctatt gctttttata
    13441 caatagctat actcgtaaag catgaaaatc gtaaatatga actttttaag ttcccccgtt
    13501 ttaataaaga aattgtcgct actacatgga gtatatcaat ttacataatg atccagaatt
    13561 ttatttcgat ggtaagctgg tttcttttct ttttgtttat tgaacactta ggagagcgct
    13621 cgctggctat ttccaatatt attcgaagta tttatatgac attaatgatc catgtctggg
    13681 cttttagctc ttcggtaaat tcgttagtga gcaactcaat cggctccggc ttgcacgata
    13741 tggttttgcc aataatacga aaagtgaata gactgagtat tctcataaca ctattggtga
    13801 tttttattac cgttactatt cccggattgt tgatcaggat ttataccgac gatattacgc
    13861 taataaatga agccataccg gtgctttttg tggtggttgg tgctgtatta ccggtcgctt
    13921 tggcaaataa ttggtttagc ggtgtgtcag gaacggcaaa gactaatgta gctcttgtaa
    13981 ttgagatttt ttgcattatc ttttatctaa cttatgtatg gttggtaatt tttaagtttg
    14041 atgtttcttt gccggttgta tggatttctg aatatgttta cattttcctt ctcggagcaa
    14101 tttcttatct ttacctaaaa tttggaaaat ggcagactaa gattatttaa ctaaaacatt
    14161 tgttatgaga agagtaagtt attccctgat tctcatcgtt attttgattt tggttagctc
    14221 gtgccgagta ggcattaaga ctactactga tataaattat caacaacaat tgcttgctgc
    14281 aacactgttt caacaatgtg cagccgagat gaaagcgctc tcatatcagg cttataatat
    14341 tgcggggatg cggctggaag aagcgctcaa agaggcaaac gatccacatc atctggcagt
    14401 aatactcgat ttggacgaaa cagtgctcga taatagccct cattcaggtc agagtattat
    14461 tgatagattc agttttccaa taggctggga cgagtggtgt tataaagccg aagctaaagc
    14521 attgccggga gcagtggatt ttttaaaaaa agcagataaa gccggtgtgc aaatttttta
    14581 tattacaaac cgtgaagaaa aacacagaga ggcaactaaa gaaaatttat taaaacttga
    14641 agttccagtt caaagcgata atcatctgat gtttaaaact gttacgagca gtaaaagaga
    14701 acggcagatg aaagtaagtg aaaattatga aatcataatg ctgattgggg ataacctcac
    14761 tgattttaca cctctttatg ataaacaatc tgttgaacaa agggataaat tgaccgacag
    14821 ccttaaaata gaatttggaa accgttttat tgttttacca aataccatgt atggcgattg
    14881 gatgtcggct attcataatt ttaatttttc actttccgat tcattaaaaa ccgtcatttt
    14941 taatgatcgt ataaaaggat ttaattttca tccaaatgaa taaatattgg ttcatattag
    15001 tttttctgtt gtttacttgt aattgtaaca gccaaactca tactgctcca caacaaataa
    15061 aggagcgtaa cgagcttgta agagtttata tcgaagggca gggagttaag gataaaaggg
    15121 taatcgcagc catgaaaaag gtagaacgtc atcgttttgt acccaataat ttactgcctt
    15181 atgcttatta tgatcagccg cttcctatag gtgaaggtca gactatttca caaccttcgc
    15241 ttgttgcttt tatgaccgag ctgcttgagc ttaagcaaag tgataaagtg ctggagattg
    15301 gtaccggatc aggatatcag gcggcaatac ttggcgaatt agttgacagt gtttttacaa
    15361 ttgagattat aaagccgttg ggagtgaaag ctgaaaagtt gataaacaac ctaggataca
    15421 gcaacatttt tgttaagatt gacgatggct acaaaggatg gccagaacat gcgccttacg
    15481 atgctattat tgttacttgc gccccgtcgg atattccaaa tcctttgcaa caacagcttg
    15541 ccgaaaatgg acggatggta atccctgtag gaaaggatgg ctctgttcaa aaattgattt
    15601 tgctaagaaa acgcaaagga aagattattc gcgaaacggt tttgcctgtc aggtttgttc
    15661 ctatgttgaa agaggatgga ggcaaatatt aagaattgat tcggccctta aaaagctttg
    15721 ttatttcttc cttttttggt ttgattattc ccttttcagt aatgattccg gttataaatt
    15781 tggctggagt aacatcaaac gccggattga ttgcttgcga gcctggcgag gcaacgcgta
    15841 ttctgttaat ttttcctgat tcgtcagggc ccgattgata gagcacttca tcttccgatc
    15901 gctcttcgat tacgatatca ttacctgttg tgcaagttaa atcaattgta gatgttggtg
    15961 cggcaataaa caaagggatt ccaaaatgtt ttgctactat tgctttttca taagttccta
    16021 ttttattggc tgtgtcacca ttggcagcga ttctgtcggc gccggttatc aatagatcaa
    16081 ctcttttatt ggacatcagc caagcgccgg cattatccgg aacaatacga tgatcaatac
    16141 ctgcattgtg caattcccag gctgttagtc ttgctccttg gcctcgtggg cgtgtttcgt
    16201 caacccatac aaaaacattt ttaccatcct ctactgatct atagattgga gcaagagcag
    16261 ttccataatc aacaaaagct agccagccgg cattacaatg ggtaagaatg tgatctccgt
    16321 tgttgattag catactaccg aacttgccaa tagcacgaga atcagctatg tctttttctg
    16381 caatttgttg tgcttgttca taagctgctt ttggcgagat taatcctgct tcatatacta
    16441 aatcaacggc atagaataaa tttcgagcag ttggacgggt atttattata gtctctttag
    16501 cttgtaatag gaatttatcc cgatttttta caggcgcagc caaaaaggct tgtgccatag
    16561 caaaacctgc tgctgcgcca atagcgccgg cgccacgggt ggtcatgttt ctaatagaat
    16621 cgcaagtggt tagaaagttt tcggagcgaa aaacaaaaaa atcgaaagga agtcggtttt
    16681 gatcaatcat cattaccgaa gctccttcca tccatattgt cctgtattct ttgccgttta
    16741 ccttcattta atttgatatt ttaccaaata aaagcatcgt catcaagcca gttaccttgc
    16801 actttaagca cttgctcgat aatatcccgc acacatcctt cacccccttt taaatgtgaa
    16861 atgtgatgcg ataaagcttt gacctcttct gccgcatcgg caggacatat cacaacgcca
    16921 gcctctttca tgatttgaag atcaggaata tcgtcgccca taaatagaac ctcttctgga
    16981 atgaggttgt gcttttttag atattcctta tataccttga ttttatgatc caccttgata
    17041 aagacatcgt taattttaag attatgcaat cgggtaacaa ttgcgtctga tgtgccaccc
    17101 gaaataactg caatgcgata cccttttttt acagcaagct gaagtgcgta gccatccttt
    17161 acattagccg tccgcaattg ttctcctgct tcggtaagga tgattttgcc gtcggtaaat
    17221 actccgtcgt aatcaaaaat catcgttgtt atttttttta ataattcttt ataattcctt
    17281 actgtcatct gctttgtttt tatctgtttc gaaaaaatta ctaatctgac tacttagtag
    17341 ttgatagatc atacttttat tagggtcgtt ttttagcatt tctaaatgct tttgcataac
    17401 cttagtgtca ttacgaatag ccgggcctgt ctgcgccatt tttgggctaa tatcgtaaag
    17461 tttttcaatg gtttcgccaa taagtggcat taaaatatcg aaggatatac cattagcttt
    17521 caggatctca tccgcattga ccatcataaa atttgtgaaa ttgcaagcaa aaacagccgc
    17581 aagatgaatt acagccctct ccttagagtt gatctgtcta acatccgagg ttatccggct
    17641 tgccaagcca atcagctttt cttcagtctg gtcgtctgat gcttcaatac aaaaaggggt
    17701 tgtggagaga ttaatgattt tttctttgat aaatgtattt aacggataga aaacgccaaa
    17761 gtggttggag gtgtttttta gtatttcaat tggtaccgaa cctgacgtat gtacaacgat
    17821 tttgttttgc aagtgtaact gtccggctat ctctgcgata aaattatcgc tgatggaaat
    17881 gatatacaga tcagcttttt ttgttagtga tgtaatatca ttagtaaagt tgcacttcaa
    17941 aagagaggca agcgctgaag cggattctat agttcggcta taaacttgta aaacagatat
    18001 tgattctttg atcaatgata agcctaactg agtggctata cgtccggcgc caataaaaac
    18061 aacggtactg attgccatga gtttttagat aatgagtgat tttaattcgc tgatcgtttt
    18121 tgccggattg gaagaagaga aaattgtatt tccggctaca agaacatctg cgccatgttt
    18181 tattagagac ggcgcatttt gcagagttac tccgccatca acttctatga gcgccttgcc
    18241 cgatttctta agaatcaact cttttagctt tgaaattttt tgaaaggtat tttcgataaa
    18301 cttttgtcct ccaaaaccgg gatttacact cattagaagt acaaaatcca cttcggcaat
    18361 tatatcgtcg agcaacatga cgtttgtatg cgggttaagt acgacaccgg ctttcattcc
    18421 taatgttttt atatgcttta tattccgatg cagatgaatg gaggcttcat aatgtatgct
    18481 gagcaggtcg gcgccacaat ttttaaaatc ttgaatgtaa cgttcgggtt ctacaatcat
    18541 caaatgaaca tcaaggggtt tggtggtcag ttgtcgtatc tgtctgatga taggctggcc
    18601 aaaagatatg ttggggacaa attttccgtc catgacatct acatgaatcc agtcggcatc
    18661 gctttgattc aacatatcga gttcggcttt aaggttggaa aaatcagccg agagcagcga
    18721 gggagctata agatgtgcca tattttaatc tttatttggc aaagttaaaa aatcacttct
    18781 aactaaaaac actaatcaca ctaattatgc ttttgttgtg gagctaattt aaataatctc
    18841 aacaaaatag ctttttaaaa attggcattt aactgatttt tgattttttt tgatcgctat
    18901 ttttttgtta ggaggtaagc gtgtcagtct tgagtttttt gcaagctctt gtattataga
    18961 ttggcgaatc tactataccg cataggatat aaattaaatc cgcgctttta gtcaaattga
    19021 agtgcaacct ttattaattt cttcaaaact tcctcgtctc tatttttttg tttttgtatc
    19081 tatggcaact tcatcctcct gggataagag ttctattttg tggattttat aaaaatactt
    19141 tttcagggta aggctttccc tataagggaa gttgctttgc tattttaagt tgccttatgt
    19201 ttcgaaatgt agatataaaa ccctgtaaat cagaatgtta tttatagaaa tgagagcaaa
    19261 ctaaagaatg ttttttaaac aaagaatttt tgtctaatta aagacattaa cttcttggcg
    19321 ggcgatccgg tcggaaacta cggttatcag aatccccttt agggcgatgg tctcgattgt
    19381 tgcgataatc tctttgtggc ttatcgcctt tttcacgtct tctactttcc tggtatcctt
    19441 caggcttagg taaaagttgt ttacgtgaga gtttaagttt cccgttttta ctatcaattt
    19501 caagaagttt tacatccact tcatccccta cttttaatac atcctcaaca ttggcaatcc
    19561 ttcgccagtc aatttctgaa atgtgaagta atccctcttt gccaggtagt atttcgacga
    19621 ttgcaccaaa tgtaataatg gattttacct tgccgcgata tgtattacct atctcgggaa
    19681 ttgctattat agcttttatt ttttcttttg ccgcttcaat tgattctttg tccaccgaca
    19741 caatatccac aatgccatat tctcctacct cttcgataac aattgttgaa ttggttgttg
    19801 cctgaatctc ttgtataatc tttccgccgg ggcctattat tgcgccaata aactctttgg
    19861 gtactgtcat ttgctcaatt ctcggaacat gtggcttgta atcttcacga ggtgtgctga
    19921 ttgttttttc tatttcgttt aatatatgca atctgccttt cagcgcttgg gcaagcgcct
    19981 cttcgagaat ttcataagat aatccgtcaa ctttgatgtc catctggcat gcggttattc
    20041 cgtctcttgt accggttact ttaaagtcca tgtcaccaag atggtcttca tcgccaagta
    20101 tatcggataa aacggcatat ttgcttccgt ccttgttggt gatcagtccc atagctatcc
    20161 ccgaaaccgg tttttcaatt ttcactccag catccattag cgctaatgtg ccgccgcata
    20221 ctgtggccat tgaggatgat ccgttagatt cgaggatatc agacacaacg cgtatagtgt
    20281 aagggtttat actcgaatcg ggtattactg gttttaatgc acgcaatgcg agattaccat
    20341 gcccgatttc tctacggctt gtgccacccc taaatctaac ttcaccagta gaaaatgatg
    20401 ggaagttata atgtaaaata aaatcgcttt tgccttcaaa tacagcgccg tcaatgacct
    20461 gactgtcaag cttggtgcca agggtaaccg ttacaagtgc ctgtgtctcg ccgcgggtaa
    20521 atattgctga tccatggaca gatggaagat agtcaacctc tgaccaaata ggtctgattt
    20581 catcaagttt ccgaccatca atacgcttac gttcatccaa cacgaaattt ctaactgctt
    20641 ctttttcaat attatgataa taacgtttta tcatagatgc tttttcaacc gcttcttctt
    20701 cctgaaatgt gtcaacaaaa tcttcgagaa tcttattgaa caactccgaa cgctgttgtt
    20761 tagctgttcc aagaccggcc acttcataac attttggata caactctgtg tacatccttt
    20821 cctttagctc ttcgtcattt gtttcatgag agtattcacg tttgttagcg gctttttcta
    20881 tcatcccggc caattcatgc tgagcttgac attgtatttt gatatggtcg tgagcgaatt
    20941 taagtacctt caacatttcg gcttccgaaa tctctttcat ttcaccttca accataataa
    21001 tatctttggt tgtagctgct acaattacgt cgagatcagc agcctccatt ttatctatcg
    21061 gaggatttat tattaactgt ccttcaatac gtgccactcg tacttctgaa attggcccgt
    21121 tgaaagggat atccgaaacc attaaagctg ctgaagcagc gagagcagca agtgcatcag
    21181 gggcatcgtt tttatcgcct gaaatcaaag taatgagtac ttgtgtttct gcatggtagt
    21241 cgtcaggaaa aagcggcctt agtgcgcggt caacgagcct tgaaattaaa atttcatatt
    21301 ctgatggtct tgcctctctt ttaaaaaagc cgccagggaa tctcccggtt gctgcatatt
    21361 tttcctgata ttcaactgtt aaaggaagaa aatcaacatc ttcatttacc tctttgcttg
    21421 aaactactgt agccagaagc atagttttgc cctgttttac gataacggcg ccatcggcct
    21481 ggcgggcaag ccggccagtt tctatactga tctctct
//
LOCUS       DAOOAI010000028        20869 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_3353_length_20869_cov_5.275344, whole genome shotgun sequence.
ACCESSION   DAOOAI010000028 DAOOAI010000000
VERSION     DAOOAI010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20869)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20869)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20869
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3353_length_20869_cov_5.275344"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="PK345_03375"
     CDS             <1..356
                     /locus_tag="PK345_03375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34094.1"
                     /translation="RKVNIIGSRFFARYYFFNSFSESSLLNKLFLHAEYEHLAYKQNY
                     KVDSNGKFVDVFGIPYSTGNETIIIPGLLVGAGIKQNIGIRTYASILFLYNLNETNNT
                     PYRNPVVRIGLGYGL"
     gene            complement(376..1839)
                     /locus_tag="PK345_03380"
     CDS             complement(376..1839)
                     /locus_tag="PK345_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-histidine dipeptidase"
                     /protein_id="HPX34095.1"
                     /translation="MNTNIAKIEPVEVWSYFSEILRIPRPSKKEERIVNFLVEFANKH
                     NLPHQIDETGNVLITKPATKGMENHKTVCLQSHMDMVCEKNKDKEFDFEKDVIKAYID
                     GDWVKADGTTLGADDGIGIATQLAILASNTIEHGPIECLFTVDEETGLSGAFGLKPGF
                     LKSTILLNLDSEDDGQIFIGCAGGRDTVVKFHFEKCTIPEDFKPVKIIVKGLKGGHSG
                     DDINKGLGNANKILVRILHRATSSYSIKLATINGGNLRNAIAREAEAVVLVKKNFIND
                     FLKQIDEFGLAIKNELKTTEPDLQIRSENTEMPEFIIDDVSQNKLINALYAAPHGVMQ
                     MSRDIPNFVETSTNLASVKLIENYFLITTSQRSSVASALDDIVNMVAAVFHLIDAEVA
                     HNDGYPGWTPNTNSEILDIAKNTYQKLFAEEPKVLAVHAGLECGLIGEKYPGMDMISY
                     GPDIKGAHSPDERISISSTARFWDWTLEILKNIPKKS"
     gene            complement(1911..2318)
                     /locus_tag="PK345_03385"
     CDS             complement(1911..2318)
                     /locus_tag="PK345_03385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34096.1"
                     /translation="MKKYISLGVNCPHCLESLMDMNYLIHGYPSIKVDIETARAKGRI
                     WLCSIYDCYDHENDIELREVEEVKFYCPHCHQSLMLDKDCSLCKAKMVGFIIKAGGRI
                     DFCSRKGCKNHYVIFENLADEIKNFYNEYGKGE"
     gene            2539..2612
                     /locus_tag="PK345_03390"
     tRNA            2539..2612
                     /locus_tag="PK345_03390"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2573..2575,aa:Met,seq:cat)
     gene            complement(2704..4314)
                     /gene="lnt"
                     /locus_tag="PK345_03395"
     CDS             complement(2704..4314)
                     /gene="lnt"
                     /locus_tag="PK345_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HPX34097.1"
                     /translation="MKQKYLYLLALLSGLILSLAWPMHGFPFLLLFGFVPLFFIEDYF
                     HNNKGQINKYAIFKYLYIAFFTWNILTTWWILNSTIFGAIMAILLNSFFMAIVFFIFH
                     LSKRTYSNPNHSIFLIVFYWIAFEYLHLDWDLSWPWLNLGNGFAMYTRWIQWYEYTGI
                     FGGTLWILISNILFFKVLKSFLIEKRINKTVAFKLAFSILVVIVPMAFSLWIYNHQIE
                     QGEKCEVIVSQPNIDPYSEQYTISIREAIEKNLVAVEPYISDSTVFIICPESALQENI
                     WHNSVEDSRSVVLTRRFLEKYSHLNIIIGASTFRLFDDDEPLTRTARKFTDVDKYYEA
                     YNTALFINQDSPVKLYHKSKLVPGPEKMPFQRLLSPLQNIAFDLGGTVGSLGLSPERE
                     IFTSLDDRFKVPGIICYESVYGSFVSGFVLNGANLLFIITNDGWWGNTAGHRQHFSFA
                     KLRAVETRRNIARSANTGISAIINSRGETLQSSDYWVSDVLKAELITNDKITFYVKYG
                     DYIGRVCTFIALFSILIAFAVSKLNWKN"
     gene            complement(4340..5545)
                     /gene="hflX"
                     /locus_tag="PK345_03400"
     CDS             complement(4340..5545)
                     /gene="hflX"
                     /locus_tag="PK345_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HPX34098.1"
                     /translation="MLQNTEINDNTEKVVLVGIITGKESESRINEYLDELEFLVETAG
                     GQTIARFTQKLEKPEPSTFIGSGKLQEVAEFVKAAKIDIVIFDDELNASQIRNIERGL
                     DCRILDRTNLILDIFARRARTSYAKTQVELAQYEYMLPRLTRMWTHLGRQRGGIGLRG
                     PGETEIETDRRIIRDRISVLKKKLEQIDKQMATQRKTRGKIVRVALVGYTNVGKSTIM
                     NQLSKSDLFAENKLFATLDTTVRKVVIYDLPFLLSDTVGFIRKLPHSLIESFKSTLDE
                     VREADLIIHVVDISHPNFEEQISTVNQTLAEIGAFDKPVIMVFNKTDAYRWIEKEEDD
                     LTPIVKENIALEDLKKTWMANFSQPTLFVSALKKENWEELKNVLYQKVKEIHISRYPY
                     TESPFYEGI"
     gene            complement(5593..6588)
                     /gene="trpS"
                     /locus_tag="PK345_03405"
     CDS             complement(5593..6588)
                     /gene="trpS"
                     /locus_tag="PK345_03405"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HPX34099.1"
                     /translation="MEIVVSGIRPTGYLHLGNYFGAIQNFLKMQDENNCFFFIADYHS
                     LTTHPTPADLHGNVKQVLVEYLASGIDPEKVTLYIQSDLPETAELYLLLNMNAYMGEL
                     ERVTTFKEKARKQPDNINAGLLTYPCLMAADIIIHKAHKVPVGKDQEQHLEMTRTFAR
                     RFNRMYNVELFPEPQAFNFGQELIKIPGLDGSGKMGKSEGEGNAIFLAEEPKSIRKKV
                     MRAVTDTGPTSPNSPKSEPVENLFSLLRIVSTPDTVQYFEDQYNSMQIRYGDLKKQLA
                     EDIISFTAPLRERIIEISADDAYLSKVVKIGADKARESAAKTVKVAREIIGFRKF"
     gene            complement(6688..7593)
                     /gene="gldA"
                     /locus_tag="PK345_03410"
     CDS             complement(6688..7593)
                     /gene="gldA"
                     /locus_tag="PK345_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated ABC transporter
                     ATP-binding subunit GldA"
                     /protein_id="HPX34100.1"
                     /translation="MSIEVSNITKIFGEQKALDDLSFSIEPGQIVGFLGPNGAGKSTM
                     MKILTCFIPPTSGQARVNGYDVMENSIDVRRVIGYLPENNPLYTEMYVKEYLEFVAGI
                     FKISNKSKRVEEMIEMTGLQIERRKKITMLSKGYRQRVGLAQALIHNPDVLILDEPTS
                     GLDPNQIVEIRNLIKTIGYKKTVMLSTHIMQEVEAICDRVIIIDKGKVVADESISKLQ
                     QINEGKEMLIVEFGHLIDSQKLKDLPQVVSVEKISDNTWKIESAGKSDIRPAIFKLAV
                     DQNTTVLTLQKQTSSLEQIFRTLTS"
     gene            complement(7674..8801)
                     /gene="dnaN"
                     /locus_tag="PK345_03415"
     CDS             complement(7674..8801)
                     /gene="dnaN"
                     /locus_tag="PK345_03415"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HPX34101.1"
                     /translation="MKFIVSSTFLLKNLQAVSGVLISNSSLPILNDFLFDLSGDNLKI
                     TTSDIETTMTISLQVSMAAESGIVAVPAKILIETLKTLPDVPVTFTVDPITLGIEIVA
                     GEGKYKMNGHNGEDFPETPVIDEGNEFTINSIALSEAISKTVFAVGIDEMRPVMTGVL
                     MEINPEFVSFVATDAHKLVRYQRFNIGVDLEDSIILPTKPLNQIKNLLGVEDQDVKII
                     YDQKNIAFEFGRVRLISKLIEGKYPNYAAVIPSDNPNKMQIERMPLLNAIRRVSIFGN
                     QSTHQLRLSISNRTLQLSAEDIDYANEAREDLNCNYNGEDMDIGFNSKFLLEMLANLD
                     TEEVLFEFSQPNRAGLIKQIVDEEVVDKDILMLIMPLMLND"
     gene            complement(9013..9651)
                     /gene="plsY"
                     /locus_tag="PK345_03420"
     CDS             complement(9013..9651)
                     /gene="plsY"
                     /locus_tag="PK345_03420"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HPX34102.1"
                     /translation="MNSELLYIIIGIVVAYLIGSIPSAVWIGRMFWGVDVRNEGSGNA
                     GATNTIRVLGIKAGIPVLLFDVFKGWLAVWLFRFLPFEMLTDYQSDLYRILISISAVL
                     GHIFPVYIGFRGGKGIATLLGVGIALYPQAVLVAVAIFIVMLILTGYVSLSSITASVT
                     FPFLVFFFFNNVSFPMLVLSVLVGIFVPITHRNNIRRLLHNEETKFRIKKNK"
     gene            complement(9644..14167)
                     /locus_tag="PK345_03425"
     CDS             complement(9644..14167)
                     /locus_tag="PK345_03425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34103.1"
                     /translation="MILICPLFTWINGQPLKVFTHDPTVFLVELNSLFQTIQNKENKA
                     KALSTFNEFSGLWNYGNYDNNHRETIYRSADLMLQRKLRAYPDFERYISALVAFKTMD
                     VSFENFDLWLKITCNLLENTNSIKSYHELLLFTEDFLESGILNKSKIFSWVAGKTPFR
                     FIIADSVFLVQIERFHLSCKTRNDSSTIFRTSGYYSPVSKEWKGKGGTVTWCRANLPE
                     NEVYADLDDYAIDMKTAAFQANNVAFHYEKYFKGSMNGHLVERVSADIVNPENALYPR
                     FESDMQSVFIEGIFQDIDYEGGFMLRGTKMIGSGFNDRPAKLIFKRLYRDKKGDYDLI
                     TARSDEFIITNEKIGSENIAVTIIHQDDSIVHTGLQLRYTHQNRQLVLYRQGPGVEQS
                     PFFDSFHKVEFDSEALYWQMDDDVIYLGAMPGLKNQSKAYFVSDNFFSEAHFDRVMGL
                     DRKHPLLWLDQYSKTFNTREFKIEQLADFIRMPESQVEVQVIRLAIQGFVHYDIAQRK
                     AFITDKVSHYIKAKSKLVDYDVIEFQSEVDSRINAEIKLENFDLSIKGVPHISLSNAK
                     KVAINPTEQEITLRKNRDFIFSGQIKAGLFDFQATECHFNYDTFRLNMPTIDTMRFKV
                     NSFETDDYGKRKLVEIKTPITNISGQMLIDHPANKSGLVLQPRYPIFTSNKESFVYFD
                     QKPEFKEAYNRDLVNYYIEPFTFENLEEFETESIRFRGFFNSGGIFPDNIDQPLEIMP
                     DYSLGFKKQTPPEGYPVYQGKGKFFNEITVNLSGLKGDGKLEYLNSTSVSKDFIFYLD
                     FVSSSNTEFNITPLTKGKTEFPPVKGLNLNQLWRPYSDSMVLQTTDLPISMFDERASL
                     KGSMILTSDALTGAGELDFLNATALSDKFAFTANAFYSDTTKLKLRSLPQSDVVFNTE
                     NYSAFVDFNTKIGNFKTNSINSKIYLPAIRYESFLNEFDWYFDSNEVELYSYAKVDES
                     GYDTLTKAELIGKPLSGAKFVSTHSKQNSLEFYSSRANYDLTNNILKATDVKLIKIAD
                     VAIYPDDGLLEIHENAVVQPLNNAIIIADTINRNHTITDAEVEIQSGSVYKAKGNYAF
                     SNFIGEVQNVTFDQISVDADSQTVATGTVGEGDHFELSPRFAFKGEISLNARNPLLNF
                     KGGYRIIQNCDSTLSRWVNFEQRIDPEDITLPVPEQLLEHGYKKLYAGFFYSNEENKI
                     YPAFLSRKNYYSDSLLFSVNGFLKNRKNGAELLIIPSSQIGLEDDEAPNPPFLSWNID
                     HCSISGRGTLNFGQDFGRLKMDLYGEIEHNIFNDTSWFNVMMTVNFHFAENAFLIMAE
                     DLASTQKPEINIENKLFKEGLTSLAGSSEVEKIYNDLTLYGQIRKIPDALLKSFIFTD
                     LKMTYHTDSRSFISKGSIGVAMVYGNPVFKYFDGYVQIVRRRSGDELNIYIELERGHW
                     YFFNYKTYQMLASSSRTDFNSAMKDMKPSARKEDERKGEPSYRFNISPDTQAKNRFLR
                     FIQQLESKEEEYE"
     gene            14525..17659
                     /gene="ccsA"
                     /locus_tag="PK345_03430"
     CDS             14525..17659
                     /gene="ccsA"
                     /locus_tag="PK345_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013725.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HPX34104.1"
                     /translation="MKNFFKSFFSMQLTIVLLLMFAMSIGIATFIENDYGSEASKIAI
                     YNATWFEILLLVLAINLIGSLFTHKLWKRKKYTTFIMHLSFVIILIGAALTRFVGYEG
                     MMHIREGKSSNQMLSDNTYITAKWGADGKETIVHKKVLMSGFGWQASPFTIKNGDEAL
                     HFKTIAFVPNAVPVVKAAVNGEVLVSLFVLTNTDRKSIILKQGESEEFSDFSLTFGEL
                     SGEKGLQLIVINDSLFFKSSDSVFQFSMGGHNVDTIPVNVLTHFAPMKLFEFEKVRFV
                     LRAFEMSGEVVAAHADIHDEGSGLNAVVLKTSNKTTEKNIIVWGKRGIVGKETHVEGY
                     GFENLTLSYGSRLLEIPFSLHLNKFILDRYPGSDSPSSYASEVTLNDTERNINYDFRI
                     FMNNILTHRGYRFYQSSYDQDEKGTILSVNHDAPGTTVTYIGYAMMAFSMIFMLVDRR
                     TRFHNLMKSISKLRDQKSKLLPAIILFLFIPISQSYAQEIKTIGNAQITVVSKAHADK
                     FSHIIVLGPGDRLEPISTLNSELLRKISGKSKFHGLTPDQVALGMMLEPQQWQHVPVF
                     KIKNKELRRILNISTNYACFIDFFEINQNYKLSSFINEAFRKNPFDQSKLDKDIISID
                     EKINVFYTMMMGGFLKVFPDINAPVAKWYHTGSEINNFPEADSNFVKNIIPVYISSLS
                     EATQTGNYDQADEFVEAIKIFQQKYAGELIPSLNHRKVEILYNRVEIFERLYKYYGML
                     GLVFLVLLFVNLVNHRFKIGFAKKAIIGLLMLLFVFQTAGFKARWYISGHAPMSNGYE
                     SMIYISWAAMLAGFIFVRKSPIALAATSILSSLTLFVAHLNWMNPEVTNLVPVLKSIW
                     LTIHVGVITASYGFLGLVMIMGLFNLLLMIFQNKKNYKNFQITIKEISMTSEMAMTIG
                     LFLITIGSFLGGIWANESWGRYWGWDPKESWALVTILVYTVILHIGYIPGIKGRYLFN
                     ALSVIGFASVLMTYFGVNYYLSGLHSYAAGDAVPIPKFVYYTLAILFVILVVAWVNSQ
                     KLKDLEIEFNSDNQ"
     gene            17742..20069
                     /locus_tag="PK345_03435"
     CDS             17742..20069
                     /locus_tag="PK345_03435"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component regulator propeller
                     domain-containing protein"
                     /protein_id="HPX34105.1"
                     /translation="MKSIFNLSALFFLLLLIPSIHAQIAIGEWRDHLPYSRTVRVAEV
                     ENMIYCATQYSIFVYDKDDQSVLRKSKVNGLSDVGISAMMYNKEYKTLVITYTNTNID
                     LIKEGKVINFSDIKRRQILGNKTINNITFNDSLTYFSCGFGIVVLDIKNEEFPEPTYF
                     IGPEGSQLNVLDITFGADSVYAATESGIYKASLNSTNLANFNEWTKDQRLYPNKSFNA
                     ITFYNGRLFTNSHIEGNTSDTLFMYDYYSHTWSILPGSSFLQKFRLNVSNEQLIEVTE
                     LGIQIYDQSFSKVATFDNPGGINLQSRDVIIDKNNTKWIADNRYGLVSVENDKSAFFI
                     TPNGPHSHKVFDMAIEDQKLWVASGGRTLSWGKMYIADGVYSFINESWKNYNGSTGYK
                     TFDTINDMTCIAINPKDNRQVFVGNWVSGVIEFFDEQVVNVYSTHNSSLQIWPTATYV
                     AVSGMDFDSHNNLWVVNSGATNLLSAKKPDGTWKGFYLGSNASSIDASKVMVDSYNQK
                     WILVRADHKLVVFSENNTFDNTSDDKVKILTNAPGNGNLPGNKIYSIAEDQDGRIWIG
                     TDQGVGVIYSPQNVFGDGNYDVQRILVEVGGYVQYLLESEVVTSIAIDGDNQKWIGTE
                     RAGVFLLSANGTEQIHHFTEENSPLFSNLIVDIAINHKSGEIFFATSRGIISYRGTAT
                     NPNPTHSDVVVFPNPVRESYTGAIAINGLVNKANVKITDINGNLVYTTKVEGGQVIWY
                     GTNMSGKRASSGVYLVFVSDDQGVEKMVTKILFIK"
     gene            20086..20808
                     /gene="recO"
                     /locus_tag="PK345_03440"
     CDS             20086..20808
                     /gene="recO"
                     /locus_tag="PK345_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HPX34106.1"
                     /translation="MISKTRGIIFKTIKYSDNSLIVKVYTELFGLRSYITRGLNKKRI
                     SINKSNLQPLSIVNILVYENGKGGLQTIKEIESAYPYRSLPFDVRKSCIALFINEVMY
                     HSIRNEIADIELFKFLYNTLINLDEIKDKYENLHLQFLINLAKYLGIAPRNNYSATNQ
                     FFDLQEGIFISEPPLHANWLNETLSQKLFQMIKQPETTPLLFTNAAARNEILFNLMNY
                     FRFHIPGFGELKSPAILSEVLT"
BASE COUNT         6548 a         3942 c         3660 g         6719 t
ORIGIN      
        1 atcgcaaggt aaatattatc ggtagtagat tttttgcgcg gtattatttt tttaatagct
       61 tttcggagtc aagcttacta aacaagcttt ttttacacgc tgaatatgaa catcttgcgt
      121 ataaacaaaa ttacaaagtt gattcaaacg gaaaatttgt tgatgtattt ggtattccct
      181 attcaacagg aaatgaaact ataattatac cgggattact tgttggtgct gggataaaac
      241 aaaacattgg tatcagaact tatgcaagta ttttatttct ttacaatttg aatgaaacca
      301 ataatacccc ttacagaaat ccggttgtca gaattgggct tggatacggt ctttaataaa
      361 tactaaggta aatggttagc tttttttggg aatattttta aggatctcca aagtccaatc
      421 ccaaaatctc gctgttgagc taatagaaat tctttcatca ggtgagtgtg ctcctttaat
      481 atccggacca tacgatatca tatccattcc cggatatttt tctccaatta aaccgcactc
      541 taaaccagca tgtacagcca gaacttttgg ctcctcagca aacagttttt gataggtatt
      601 ttttgcaata tcgagtattt cagagttagt gtttggggtc catcccggat agccgtcatt
      661 atgagcaact tcggcatcta tcaaatgaaa aactgctgca accatattta ctatatcgtc
      721 aagggcagat gccactgaac tcctttggct tgtggtgata agaaaataat tttcgataag
      781 tttcactgat gccagattag tggaggtttc tacaaaattt ggaatgtctc tcgacatttg
      841 cattactcca tgaggtgcag catagagtgc attaattaat ttgttttgtg acacatcatc
      901 aataataaat tccggcattt cagtattttc tgatcttatc tgtaaatcgg gttcggtggt
      961 tttcaattca tttttaattg ctaaaccaaa ttcatctatt tgttttaaaa aatcattaat
     1021 aaaattcttt tttactaaaa ccacagcttc agcttcacgt gcaatggcat tacgcaagtt
     1081 gcctccatta atagtagcga gctttatgct ataacttgaa gttgcccgat gtaaaatacg
     1141 aactaagatt ttgttggcat ttcctaaacc tttattaata tcatcgccgg aatgccctcc
     1201 tttaagtcct tttacgatta tcttaacagg tttaaagtct tccggtattg tacatttttc
     1261 aaaatgaaac tttacaaccg tatcccgtcc gccggcacag ccaataaaaa tttggccatc
     1321 atcttctgag tcaagattaa gcagtattgt tgattttaaa aatccaggct ttaatccaaa
     1381 tgcgccggat aaccccgtct cttcgtcaac ggtaaataaa cactcaatag ggccatgctc
     1441 aatagtgttt gaagccaaaa tagcaagttg agtagctatg cctattccat cgtcagctcc
     1501 aagggtagtt ccgtcagcct tgacccaatc gccatcaatg taagccttga tcacatcctt
     1561 ttcaaaatca aattctttat ctttattttt ttcacaaacc atatccatat ggctctgtaa
     1621 acacacagtc ttatgatttt ccatcccttt ggtggcaggc ttcgtaatta aaacattacc
     1681 ggtttcatct atctgatgag gtagattgtg tttgttagca aattctacaa ggaaattgac
     1741 aatacgttct tccttttttg atggacgagg tattcgaaga atttcactaa aataactcca
     1801 tacttcaact ggttcaatct ttgcaatatt agtgttcata ctaaaaaact aaatttttga
     1861 ttattgattt atttttaaga ttttaaaagt taattacgga tgcaaagctc ttattcgccc
     1921 tttccgtatt cattataaaa gtttttgatt tcgtcggcaa gattttcaaa aatcacataa
     1981 tgatttttac accctttacg agagcaaaaa tcaatacggc ctccagcttt tataataaaa
     2041 ccaaccattt ttgctttaca caacgaacaa tctttatcaa gcataaggct ttggtggcaa
     2101 tgaggacaat aaaatttaac ctcctcaact tcacgcaatt caatatcgtt ttcatgatca
     2161 taacaatcgt aaattgaaca tagccaaatt ctgcctttag ctctcgcggt ttcaatatcc
     2221 actttgatac tcggatatcc atgaataagg taattcatat ccattagcga ctctaaacag
     2281 tgagggcaat ttacccccaa agagatatat tttttcatct ttcaaaaatt ttaagtttag
     2341 acatttttaa aatttgtggt aaaaataata aataacgggt gaatctcaca ttctttttaa
     2401 tgaagattta ctttatactt aaaaacgatt attaattgtc tgatttacat tatattatga
     2461 acattgccca aattttccac tatttttttc aacgcacctt taaatagttt tctatttttg
     2521 cagcccattt tataaaatgg cgagatagct caggtggtca gagcgcagca ctcataatgc
     2581 tgaggtcgag ggttcgagcc cctctctcgc tactatccta aagcgctaac tatcaatggt
     2641 tggcgctttt ttttataaac atcaaaatcg cagatcaagc acttgacaat atttacgcag
     2701 agtttaattt ttccaattta atttcgacac agcaaaagct atcaaaattg aaaaaagagc
     2761 aatgaaagtg catacacgtc caatataatc accatatttt acgtaaaatg taattttatc
     2821 attggtaatt aattcagctt tcaaaacatc cgacacccaa taatcggaag attgaagagt
     2881 ttctcctctc gaattgatga ttgcagaaat accagtattt gcagatcggg caatattacg
     2941 acgggtttcc actgctcgta attttgcaaa tgaaaagtgc tgacgatgtc cagcggtatt
     3001 accccaccag ccgtcgttgg tgataatgaa caataaatta gcgccattga gaacaaatcc
     3061 cgatacaaaa gagccatata ctgactcata gcaaattatt cccggaactt taaatctgtc
     3121 atctaatgat gtaaatattt ctctctcagg tgataaccca agactaccta cagttccacc
     3181 aaggtcaaaa gcgatattct gaagtgggct taataaacgt tgaaacggca ttttctcagg
     3241 gccgggaaca agttttgatt tatgataaag cttaacggga gagtcttggt ttataaaaag
     3301 cgctgtatta tatgcttcgt aatacttatc tacatcagta aactttcttg ccgtgcgggt
     3361 caatggctca tcatcatcaa acagacgaaa agtagaggct ccaattatta tattaaggtg
     3421 tgagtatttt tccaaaaagc gtcgtgtcaa tacaacactt cggctatcct caacagagtt
     3481 gtgccatatg ttttcttgca gtgccgactc ggggcaaata ataaaaacag tgctatcgga
     3541 aatgtatggc tccaccgcta caaggttttt ctctatagcc tcccggatgg aaattgtgta
     3601 ttgctcggaa taaggatcta tatttggctg acttacaatt acttcgcact tttctccttg
     3661 ctcaatctga tgattgtata tccacaagga aaaagccatt ggaacaatca caacaagtat
     3721 cgagaaagct aatttaaatg caactgtttt atttatcctt ttttcaatta aaaatgactt
     3781 caatacttta aaaaacagta tgttacttat taaaatccac aatgttcctc caaaaattcc
     3841 agtatattca taccattgta tccaacgggt gtacattgca aaaccattcc caagatttaa
     3901 ccagggccag gaaagatccc agtcgagatg cagatactcg aaagcaatcc agtaaaatac
     3961 aataagaaat atcgaatgat taggattgga ataggttctt ttcgaaagat gaaatataaa
     4021 aaaaacaata gccataaaaa aggagttgag caagatggcc attattgctc caaaaatagt
     4081 tgaattcaat atccaccagg ttgtcagaat attccacgta aaaaaagcga tgtacaaata
     4141 tttaaagatg gcatatttat taatttgacc cttgttatta tgaaaataat cttcgataaa
     4201 aaacaaggga acgaatccaa aaagaagcaa aaacggaaag ccatgcattg gccaagccag
     4261 gctaagaatc aatcctgaca atagagctaa aagataaagg tatttttgtt tcactcctga
     4321 tttattttat gattaaaagt cagatacctt cataaaacgg actttccgta taagggtatc
     4381 gtgaaatatg aatttcttta actttttggt acaacacatt ttttaattct tcccagtttt
     4441 ctttttttaa tgctgataca aacagcgttg gctgggagaa attggccatc catgtctttt
     4501 tcaaatcttc taaagcaata ttctctttga caattggtgt aaggtcatct tcttcttttt
     4561 caatccaacg gtatgcatca gttttattaa acaccattat tacaggctta tcaaaggcgc
     4621 caatttcagc aagagtttga tttacagtgc ttatttgctc ttcaaaatta gggtgtgaaa
     4681 tatcaacgac atggattatt aggtctgctt ctcttacttc atccagagtt gacttaaaag
     4741 attcaataag gctatgaggt aactttctga taaaacccac agtatcagag agtaaaaatg
     4801 gtagatcgta aataacaact tttcttactg tggtatccaa ggtggcaaaa agtttgtttt
     4861 cggcaaaaag gtcagacttg ctaagttgat tcataattgt agatttacca acattggtat
     4921 atcctacgag cgctactcta actatttttc cacgagtttt gcgctgcgtt gccatctgtt
     4981 tatcaatctg ttctaatttt tttttcagca cgcttattct atctcgaata atccgacggt
     5041 cagtctctat ttccgtttcg cctggcccac gtaaaccaat tcctcctctt tggcgtccaa
     5101 ggtgtgtcca catacgggta aggcgtggaa gcatatattc atactgagcc aactctactt
     5161 gtgttttggc atacgacgtt cgggcacgac gcgcaaaaat atcgagtata aggttagtcc
     5221 gatcaagaat tctgcaatcc aatccccgtt caatattacg tatttgagaa gcattgagtt
     5281 catcatcaaa tattacaata tctattttgg ccgctttaac aaattcggct acctcttgta
     5341 atttgcctga accaataaaa gtagatggct caggtttttc aagcttttgc gtgaatcgag
     5401 caatggtttg tccaccggcg gtttcgacca gaaattccaa ctcgtcaaga tattcattaa
     5461 tacgactttc agattctttt cccgtaatta tacctaccag aactaccttt tcggtgttat
     5521 cattaatttc ggtgttctga agcataatat tttgaaactg atttttttta gataatcaca
     5581 atatgccaag atttaaaatt ttcgaaatcc gattatctct ctagcaactt ttactgtttt
     5641 agcagcactt tctctcgctt tatcagctcc tatttttacc actttactca gataagcgtc
     5701 atctgccgaa atttcaataa tcctttctcg aagcggtgcg gtaaaagaga taatatcttc
     5761 agcaagttgt ttttttaaat caccatatcg tatttgcatt gagttgtact gatcttcaaa
     5821 atattgtact gtatcaggcg ttgaaactat cctaagcaaa gaaaaaagat tttccaccgg
     5881 ttctgatttt ggagaattgg gagatgtggg gccggtatca gtaacagctc tcatcacttt
     5941 tttacgaata gatttaggct cttcggctaa gaaaatagca tttccttcgc cttcagattt
     6001 tcccattttt ccgctaccat ccagtcctgg tattttaatc aactcctgac caaaattaaa
     6061 tgcttggggc tcagggaaca attctacatt atacatcctg ttaaagcgtc gggcaaaagt
     6121 ccttgtcatc tcaaggtgtt gttcctgatc tttccctaca ggtaccttat gcgctttatg
     6181 aataataatg tctgctgcca taaggcatgg ataggttagt aggccggcat tgatgttatc
     6241 gggctgctta cgagcctttt ccttaaacgt tgttacacgt tccaactcgc ccatataggc
     6301 attcatgtta agcaaaagat ataattcagc agtttcaggc aaatcacttt ggatatagag
     6361 tgtaactttt tcaggatcaa tcccggaggc caaatattct accaatacct gtttcacgtt
     6421 tccgtgcaag tcagcaggcg ttggatgagt agttaatgaa tgataatcag caataaagaa
     6481 aaaacagtta ttctcatcct gcatttttaa aaaattctga atcgcaccaa aataattgcc
     6541 caaatgcaaa tatccagtag gtcgtattcc acttactact atctccatat tttattaaaa
     6601 aaaggtattt taatgttggg gcaaaagtaa taaaatccat tctcctcaaa cgacagtaga
     6661 aatcgtatgg tagtaataac cttttaatta gctagttagt gttctgaaaa tctgttcaag
     6721 tgaagaagtc tgtttttgaa gtgtgagcac agtagtgttt tgatccacag ccagtttaaa
     6781 aattgccggt cgtatatcag attttccggc actttcaatt ttccaggtat tgtcgcttat
     6841 tttttcaact gacacaactt gtggcaagtc cttaagtttt tgcgaatcaa ttaaatggcc
     6901 aaactccact atcaacattt cttttccttc attaatttgc tgaagttttg atatgctttc
     6961 gtcagcaaca acttttcctt tatcaataat tatcactctg tcgcaaattg cctctacttc
     7021 ttgcattata tgtgttgaca acataacagt ttttttgtag cctatcgttt ttattaagtt
     7081 tcttatttct actatctgat taggatcgag gccggaagta ggttcatcga ggataagtac
     7141 atcgggatta tggataagcg cctgtgcaag tccaacgcgt tgacgataac ctttggaaag
     7201 catggtaatt tttttcctgc gttcaatttg caagcctgtc atctcaatca tttcttccac
     7261 tcttttcgac ttattggaaa tcttaaaaat cccagctaca aattccagat attcctttac
     7321 atacatttcg gtataaagcg gattattttc cggcaaataa cctattaccc tgcgtacatc
     7381 tattgagttt tccatcacat catacccatt tacccgggcc tgaccggaag ttggagggat
     7441 aaaacaggtc aggattttca tcattgtaga ttttccagcg ccattgggac caagaaatcc
     7501 cactatttga cccggctcta ttgaaaaaga gaggtcgtca agcgccttct gttcaccaaa
     7561 gatttttgta atattactta cttcaataga cataatcggg tatcagatta tggggcaaat
     7621 actgaaaatt ttcagtttct acctaaaaaa gatgttatta aaacaccaaa tcatcaatca
     7681 tttaacatca gcggcattat gagcatcaaa atatctttat caacaacttc ttcgtcaact
     7741 atctgtttaa tcaatcctgc tctgttaggt tgtgaaaatt cgaaaagcac ctcttctgta
     7801 tctaagtttg caagcatttc gagcagaaat ttcgaattaa accctatatc catatcttca
     7861 ccgttgtagt tacagttaag gtcttcacgt gcttcattag cataatctat atcttcggcc
     7921 gaaagctgta gtgtccgatt agatattgaa aggcggagtt gatgggttga ttggttacca
     7981 aaaatactta ccctacgaat agcattgagc aatggcatac gctctatctg cattttattg
     8041 ggattatcac ttggtattac tgcagcataa ttaggatatt ttccttcgat cagcttacta
     8101 atcaatcgta cacggccaaa ctcaaatgct atatttttct gatcatatat tattttaaca
     8161 tcctgatctt caacaccaag aagatttttg atttgattaa gtggtttggt gggcaggata
     8221 atagaatcct ctaagtcaac tcctatatta aatcgctggt aacgaaccaa tttatgtgca
     8281 tcggtggcaa cgaaagagac aaactccgga tttatttcca tcaaaacacc tgtcattaca
     8341 ggtcgcattt catcaatacc aacagcaaaa actgttttcg aaatggcttc agagagcgct
     8401 atagaattaa tagtaaattc attaccttcg tcaattaccg gtgtttcagg aaaatcttcg
     8461 ccattgtgtc cattcatttt atactttcct tcgccggcaa ctatctcaat accaagtgtg
     8521 ataggatcca cagtaaaggt aacaggcaca tctggcaatg tctttaaagt ttctattaat
     8581 attttggccg gtacagcaac tattccggat tcagcagcca ttgacacttg aaggcttatt
     8641 gtcatggttg tttcaatgtc ggaagtggta attttcaggt tatcacccga aagatcaaaa
     8701 agaaaatcat tcagaatagg taatgagctg tttgaaatca agacgccact tacagcctga
     8761 agatttttta gtaagaatgt gctggagaca ataaatttca tcttcgtgtt tatttaatgg
     8821 ttaattactt taaaaataat ggtttaagat ttattaatga tatcgcgttt aaaaaaatta
     8881 tttatcaatt ttctgaaaag tcaaaaatag gtcaattttt taaaatctct gcaaagataa
     8941 aacctgccac cgaaaaaaag tcaataaaca aaatattatt tattaaaaac attgattatt
     9001 aacctacttg aattatttat tttttttaat tcgaaatttc gtctcttcat tatgtaataa
     9061 gcgtcgtatg ttgtttcgat gtgttatcgg tacaaaaatt ccaactaata ctgataagac
     9121 aagcattgga aatgatacgt tattaaagaa aaaaaacact aaaaacggga atgtaacaga
     9181 agcagtaata gatgataggg atacatatcc tgtaaggatc agcataacga taaatattgc
     9241 cactgcaacc aaaacggcct gtggataaag tgcgatacct acaccaagta aggtagctat
     9301 tcctttacct cccctaaagc caatatagac gggaaaaata tgccctaata cagcagatat
     9361 tgatattaaa atccgataaa gatctgactg atagtctgtc aacatttcaa agggaagaaa
     9421 tcgaaaaagc caaaccgcca accaaccttt aaacacatcg aatagcaaaa cgggtattcc
     9481 tgctttaatc ccaagcactc tgatagtatt agtggcgccg gcattgccgc tgccttcatt
     9541 tcgtacatca acaccccaaa acatcctgcc gatccaaact gccgatggta ttgagccaat
     9601 aagataagct accacaatac caattattat atataacaac tcactattca tactcttctt
     9661 ctttagattc aagttgttga ataaaacgta aaaatctgtt tttcgcttgt gtatcaggag
     9721 aaatattaaa acgataagag ggctcgcctt ttctctcgtc ctcttttcga gccgacggtt
     9781 tcatatcttt cattgcggag ttaaagtcgg ttcgtgaaga acttgcaagc atctgatacg
     9841 ttttatagtt gaaaaaatac caatgacctc gttcaagttc aatataaata ttcaattcat
     9901 ccccactacg tctgcgcacg atttgaacat aaccgtcaaa atatttaaac accggattcc
     9961 cataaaccat tgccaccccg atcgaacctt ttgagataaa agaacgtgaa tcggtgtgat
    10021 aagtcatctt taaatcggta aaaataaaac tctttaacaa ggcatcaggg attttacgaa
    10081 tttgtccgta aagtgtcaga tcgttataaa ttttttctac ctctgatgat ccagccaaag
    10141 aagtgagtcc ctctttaaat agtttatttt caatattaat ctctggtttt tgggtagatg
    10201 caaggtcttc ggccataatt aaaaatgcat tttcggcaaa atgaaaattg actgtcatca
    10261 tgacattgaa ccacgaggta tcgttaaaaa tattgtgttc tatttcccca taaaggtcca
    10321 tctttaatcg cccaaaatcc tgcccgaaat ttagcgtacc tcttccggaa atactgcaat
    10381 ggtcaatatt ccagcttagg aagggagggt taggcgcttc atcatcctcc aaaccaatct
    10441 gacttgatgg gataattagc aactctgcgc cgtttttacg gttttttaaa aatccattga
    10501 cggaaaaaag cagtgagtcg ctataataat ttttcctgga tagaaaagca ggataaattt
    10561 tgttttcttc gtttgaataa aaaaaaccag cataaagttt tttataacca tgttcaagta
    10621 gttgttccgg cactggtaac gttatatcct cagggtcaat acgctgttca aagtttaccc
    10681 atctcgacag ggtagaatcg cagttttgaa taatacgata gcctccttta aaatttaata
    10741 atgggttacg agcattcagg cttatttctc ctttaaaggc aaaccggggg cttaattcaa
    10801 aatgatcccc ctcgccaact gtaccagtag caaccgtttg gctatcggca tctacgctaa
    10861 tctgatcgaa agttacattt tgcacctctc caataaaatt gctaaatgcg taattacctt
    10921 ttgctttgta aacagaaccc gattgtattt caacttccgc atcggttatt gtatgatttc
    10981 ggtttatagt atcagcaatt attattgcgt tatttaaagg ctgaacaaca gcattttcat
    11041 gtatctccaa caaaccatca tcagggtaaa tggctacatc tgcaatttta ataagtttta
    11101 cgtcggtagc tttcaaaata ttattcgtca aatcgtaatt ggctctgctt gagtaaaatt
    11161 ctaatgaatt ctgctttgag tgtgtagata caaatttcgc tcccgataat ggttttccta
    11221 ttaattcggc cttcgttaaa gtatcgtagc cggattcgtc aaccttggca taactatata
    11281 gctccacttc attgctatcg aaataccaat caaattcatt cagaaaagat tcataccgga
    11341 ttgcgggcag ataaattttt gagttaatac tatttgtttt aaaattaccg atttttgtgt
    11401 taaaatccac aaaagcgcta tagttttctg tattaaaaac cacatcggat tggggcaaac
    11461 ttcttaattt gagctttgtg gtatctgaat aaaatgcatt agctgtaaat gcaaatttgt
    11521 ccgacagggc tgttgcgttt aagaaatcaa gctctcccgc gccggttaaa gcgtcagatg
    11581 ttaatatcat cgaacccttt aatgacgctc tttcatcaaa catagaaatg ggtaaatccg
    11641 tggtttgcaa aaccatagaa tctgaatacg gcctccagag ttggtttagg tttaatcctt
    11701 taacaggtgg aaattcagtt tttcctttgg tcaaaggtgt aatattaaat tcggtatttg
    11761 atgatgaaac gaaatcgaga taaaagataa aatccttgct aacagatgta gaattaagat
    11821 attcaagttt cccatctcct tttaatccgg ataaattaac tgtaatttca ttaaaaaact
    11881 ttccttttcc ctgataaaca ggatatcctt cgggtggtgt ttgtttttta aatcccaacg
    11941 aataatctgg cattatttca aggggttggt caatattgtc aggaaaaatc ccacctgaat
    12001 taaaaaaacc tctaaatctg attgattctg tttcaaattc ttcaagattt tcgaaggtaa
    12061 agggttcaat ataataattg accaaatcgc ggttataagc ctctttaaat tcaggttttt
    12121 gatcaaaata gacaaaagac tccttgttac ttgtaaatat cggataacga ggctgcaaaa
    12181 ccaatccgct tttattagcc ggatggtcaa ttagcatttg cccgctgata ttagtaatcg
    12241 gagttttaat ttctacgagt tttcttttgc cataatcatc ggtttcaaaa gagttgactt
    12301 taaaacgcat ggtatcaata gtcggcatat taagcctgaa tgtgtcataa ttaaaatgac
    12361 attcggtagc ttgaaaatcg aataggcctg cttttatttg ccctgaaaag ataaaatcac
    12421 ggttttttcg taaagtgatt tcctgttcag ttggatttat tgcaaccttt tttgcgttgc
    12481 taagtgaaat atgaggaaca ccttttatag agagatcgaa attttcgagt tttatttccg
    12541 cattgattct tgaatctact tccgattgaa attcaataac atcataatca acaagtttac
    12601 ttttagcttt gatataatga cttaccttgt cggtaataaa agccttgcgt tgggctatgt
    12661 cgtaatgcac aaagccttgt attgcaagcc tgataacttg tacttccacc tgactttccg
    12721 gcatccttat aaaatctgcc agttgttcaa ttttaaattc tcttgtgtta aaggttttgc
    12781 tgtattggtc taaccaaagc aaaggatgtt ttcggtcgag ccccatcacc ctgtcgaagt
    12841 gtgcttcaga aaaaaaatta tctgaaacaa aataggcttt actttgattt tttaacccag
    12901 gcatagctcc aagataaata acgtcatcgt ccatctgcca ataaagagct tcggagtcga
    12961 attcaacttt atggaaacta tcaaaaaatg gactttgttc tactccaggt ccttgtcggt
    13021 ataaaacaag ttgacgattt tgatgagtat atctgagctg caatccagta tgaactattg
    13081 aatcgtcctg atgaataatt gtaacagcga tattctccga tccaattttt tcattggtga
    13141 ttataaactc atcggaacgg gcagttatca aatcgtaatc ccctttctta tcccgataaa
    13201 gccttttaaa aatcaatttt gccggccgat cattaaaacc acttcctatc atttttgttc
    13261 cgcggagcat aaaacctcct tcataatcaa tatcctgaaa aattccttca ataaacacac
    13321 tttgcatatc cgattcaaac cgcggataaa gtgcattttc aggatttaca atatctgctg
    13381 atacacgttc taccaaatgc ccattcattg agcctttgaa atatttttcg taatgaaatg
    13441 caacattgtt tgcctgaaaa gcagctgttt tcatgtcaat tgcgtaatca tccagatcgg
    13501 cataaacttc attttcagga aggtttgccc gacaccaggt aaccgtccca cccttacctt
    13561 tccattcttt tgatacaggg gaatagtaac ccgacgttct gaaaatcgtt gaagaatcat
    13621 ttcgtgtttt acatgaaaga tgaaaccgtt cgatctgcac taaaaaaaca gaatcagcga
    13681 taataaagcg aaaaggagtt ttgcccgcta cccacgaaaa gatctttgac ttgtttaaaa
    13741 tacctgattc aaggaaatct tcggtaaaca ataataattc atgatatgac tttattgaat
    13801 ttgtattttc caacaaatta caggtgattt ttagccaaag atcaaaattc tcaaaactta
    13861 catccatcgt tttaaatgca accagagccg aaatatatcg ttcgaaatcg ggatatgccc
    13921 ttaatttcct ttgcaacatc aggtctgcgc ttctgtatat tgtttcccga tgattattat
    13981 cataattccc gtaattccat aatccggaaa actcattgaa ggttgataag gctttggctt
    14041 tgttttcttt attctgaata gtttgaaaaa gtgaatttag ttctaccaaa aacacggtag
    14101 gatcatgagt aaataccttt aaaggctgtc cattaatcca tgtgaataat gggcagatca
    14161 aaatcattaa caaaactatt cgcctgtaca tttttccgat aaaaaaacat tgttaaccat
    14221 ctggaataaa gcgataaaag gttagtttta ttgtttcact cctttagtta taaagaggtc
    14281 ggaaaattct aaaaaaaatt acctaaaact aattaataga aaagcttcat ggctcaagcc
    14341 actgatatta tgttaatttg gtgtgtttta gctgtaagtc aattcaagta ttacatttcc
    14401 aaaataaagt atttttgccc gctctgtaat agtttttcct tgtgatgatt tacctcacaa
    14461 ggttttaaaa agatcagact ccactacttc atatcaatca ttgattaaaa cagaaatttt
    14521 gataatgaaa aattttttta aatccttctt ttcgatgcaa ttaacgatag ttttattgtt
    14581 aatgtttgcg atgtcaatcg gcatagctac ttttatcgaa aacgattatg gatctgaagc
    14641 ctcgaaaatt gcaatttata atgccacctg gtttgagata ttactattag tattagcgat
    14701 aaaccttatt ggaagccttt ttacacacaa attatggaag cggaaaaaat acaccacatt
    14761 catcatgcat ttatcatttg tgattattct tattggcgca gccctcacaa ggtttgttgg
    14821 ctacgaaggc atgatgcata taagggaggg taaatcgtcc aatcagatgc tgtcggacaa
    14881 tacctatata actgcaaaat ggggcgccga tggaaaagag accattgttc acaaaaaggt
    14941 tttaatgtcc ggttttggtt ggcaagcatc gccatttacc ataaaaaacg gagatgaggc
    15001 attacatttt aaaactatcg cttttgtgcc caatgccgtt ccggtggtaa aggctgccgt
    15061 taatggagag gtacttgttt cgctttttgt tcttactaat accgatagaa aatcaatcat
    15121 tctcaaacag ggtgagtcag aggagttttc agatttttcg ctcacgtttg gcgagctatc
    15181 aggagaaaaa gggttgcagc tcatagttat taatgatagt ctatttttca aatcttctga
    15241 ttctgttttt caattttcga tgggagggca taatgtagat acgataccag taaatgttct
    15301 tactcatttt gctccgatga aattgtttga atttgaaaaa gtccgttttg tattgagagc
    15361 ttttgagatg agtggagaag ttgttgctgc tcatgccgat attcacgacg aagggtcagg
    15421 actgaacgct gttgtgctta aaacttcaaa caaaaccacc gaaaaaaaca ttattgtgtg
    15481 gggtaagcgc ggtattgttg gaaaagagac gcatgttgaa ggttatggat ttgaaaatct
    15541 gactttatcc tatggttcac gcttattgga aatacctttt agtttacatc ttaacaagtt
    15601 tattcttgat cgttatccag gctctgacag cccctcctct tatgccagcg aagttacttt
    15661 aaacgacacc gaaagaaata tcaattatga ctttcgaatt tttatgaata atattttaac
    15721 gcatcgcgga tatcgttttt accaatcctc ttacgatcag gatgaaaaag gcacaatact
    15781 ttcagttaat catgatgcgc ccggcacaac cgttacctat attggctatg caatgatggc
    15841 gttcagtatg atttttatgt tagttgaccg tagaactcga ttccataatc taatgaaatc
    15901 cataagtaaa ttaagggatc aaaagagtaa actgctcccg gcaattatcc ttttcctttt
    15961 tatcccaatt tcgcaatcat acgcccaaga aataaaaact attggtaacg cacaaattac
    16021 cgtcgtatcc aaagctcacg ctgataaatt ttcacatatc atcgtacttg ggcccgggga
    16081 tcgtttagag ccgataagta ctttgaatag tgaactgttg agaaagatat cgggaaaaag
    16141 taaatttcac gggcttactc ccgatcaggt agcgttgggc atgatgcttg agccgcaaca
    16201 atggcagcat gttcctgttt ttaagataaa aaacaaggaa ttgcgtagga ttctcaatat
    16261 atcaactaac tatgcctgtt ttatcgattt tttcgaaata aatcaaaatt acaaactgag
    16321 ctcatttatt aatgaagcat ttaggaaaaa tccatttgac cagagcaaac tcgacaagga
    16381 tatcatttcg attgacgaga agataaatgt gttttacacc atgatgatgg gtggtttttt
    16441 aaaagtattc cctgatataa atgctcccgt tgccaaatgg tatcacactg gttcggagat
    16501 taataatttt cctgaggctg acagtaactt cgttaaaaac attatccctg tttatattag
    16561 ttcactatcg gaagccacac aaactggaaa ctacgaccag gccgatgaat ttgttgaagc
    16621 tattaagatt ttccagcaaa aatatgctgg agaactgata ccatcgttaa atcaccggaa
    16681 agttgagatc ttgtataatc gagttgagat ttttgaacgg ttgtacaaat attacggtat
    16741 gctcggattg gttttccttg ttttgttgtt tgtcaacctt gttaatcata gatttaaaat
    16801 aggatttgca aaaaaagcca taataggcct attgatgctt ctttttgttt ttcaaactgc
    16861 cggttttaaa gctcgttggt acatttcggg tcatgcgcct atgagtaacg gttacgaatc
    16921 catgatatat atctcttggg cagcgatgct ggcgggtttt atattcgtca gaaaatcacc
    16981 tattgcttta gcagccacat caatactttc gtctcttact ctttttgttg cacatctaaa
    17041 ctggatgaat cctgaagtta caaaccttgt accggtactt aaatcaatct ggcttacgat
    17101 tcatgtaggg gtaattacag caagttatgg gtttcttggt ttggtgatga ttatggggct
    17161 ttttaacctg ttactaatga tttttcagaa caaaaaaaat tacaaaaact ttcagataac
    17221 tattaaagag atatcaatga caagcgaaat ggctatgacc attggccttt ttctgattac
    17281 cattggctca tttctgggtg gtatatgggc taatgaatca tggggacgct attggggatg
    17341 ggatccaaaa gagagctggg cgcttgttac tattcttgtt tacaccgtaa ttttacatat
    17401 tggatatatc cccggaatta aagggaggta tctgttcaat gcattatcgg ttattggttt
    17461 cgcatcggtg ttaatgacct attttggggt gaactattat ttatcagggc ttcattccta
    17521 tgctgccggc gatgctgttc cgataccaaa gtttgtatat tatactctgg ctatcctgtt
    17581 tgtcatcttg gttgtggcat gggtcaacag tcaaaagtta aaagatcttg aaatcgaatt
    17641 caattctgat aatcaataat atttattttt cataactcat tgagtttaaa agcaattttt
    17701 gacagcaaaa tcagtttaaa atttatcctt tacgaacaac aatgaagtca atttttaatc
    17761 tttccgcact gtttttttta ttattgctaa tcccttctat acatgctcaa atagcaatag
    17821 gcgaatggcg cgatcatttg ccatactcgc gaaccgtaag agtggctgaa gtagaaaaca
    17881 tgatctactg cgcaactcaa tatagcattt ttgtttatga taaagatgac caaagcgttt
    17941 tgcgcaaatc aaaagtaaac gggctttcgg atgtgggtat tagcgccatg atgtataaca
    18001 aagaatacaa aacccttgtg attacctata ccaacacaaa tattgatttg attaaagagg
    18061 ggaaagttat caatttttcc gacatcaaac ggcggcagat attgggtaat aaaacaatca
    18121 ataacatcac tttcaacgat agtcttactt atttttcttg cggttttggc attgtcgttt
    18181 tggatattaa aaatgaagaa tttcctgaac cgacatattt tattggacct gaaggcagtc
    18241 agttaaatgt gttggatatc acttttggcg ccgattcggt gtatgcagcc actgaaagcg
    18301 gtatttacaa agcatcctta aacagcacca atcttgccaa tttcaatgaa tggacaaaag
    18361 atcaacgact ataccctaac aaaagcttca acgccatcac tttttataat ggccggctgt
    18421 ttaccaatag tcatattgaa ggtaacactt ctgacacatt atttatgtac gactattact
    18481 cacacacctg gagtatatta cccggcagca gttttttaca aaagtttagg ttaaacgttt
    18541 ctaacgaaca acttattgaa gttacagaat tgggcattca aatctacgat cagagttttt
    18601 caaaagtagc tacatttgac aatccgggtg ggataaattt acaatcgcgc gatgtcatta
    18661 ttgataaaaa caataccaaa tggattgccg acaatcgtta tggtttagta tcagtggaaa
    18721 atgataaaag cgcctttttt attaccccca acggaccgca ttcacataag gttttcgata
    18781 tggccattga agatcagaaa ctgtgggttg catctggtgg cagaacgttg agctggggca
    18841 aaatgtatat cgccgatggt gtctattcgt tcattaacga atcctggaaa aattataacg
    18901 gtagcactgg atacaaaact tttgatacca taaacgacat gacctgtatt gctatcaatc
    18961 caaaagataa ccggcaagtc ttcgttggaa attgggtaag cggagttatt gaattttttg
    19021 atgaacaggt agtcaacgtt tatagcaccc acaacagctc gttacagatt tggcctacag
    19081 caacatatgt agcagtgagc gggatggatt ttgatagcca taacaacttg tgggttgtca
    19141 attcaggagc aacaaattta ctatcagcaa aaaaacctga cggcacgtgg aaaggtttct
    19201 atctcggttc aaatgcaagt agcattgatg cttcaaaggt gatggttgac agttacaatc
    19261 aaaaatggat attggttaga gccgatcata aattggtggt attcagcgaa aacaatacat
    19321 ttgataacac ctcagacgat aaagttaaaa tacttaccaa cgcacctggg aatggtaatt
    19381 tgcccggcaa caaaatatat agtattgctg aagatcaaga cggcagaata tggattggta
    19441 ctgatcaggg ggttggtgtg atctattcgc cacaaaacgt cttcggtgat gggaactatg
    19501 acgtgcagcg tattcttgtt gaggttggtg gttatgttca atatttactt gagtcagaag
    19561 ttgttacttc tattgccata gatggcgaca accagaaatg gataggaaca gaacgtgctg
    19621 gagtttttct gttatcggcc aatggcacag agcaaattca tcattttact gaggaaaaca
    19681 gccctttatt ttccaatcta atcgttgata ttgctatcaa ccacaaaagc ggagaaatct
    19741 tttttgcaac cagtagaggc ataatttcat atcgaggaac tgctacaaac ccgaacccca
    19801 cacattctga tgttgtggtt tttccaaacc ctgtaagaga aagctatacc ggagctattg
    19861 ccatcaacgg attggtaaac aaagccaatg ttaaaattac cgatatcaat ggtaatttag
    19921 tgtacacaac caaagtagaa ggagggcagg taatatggta tgggaccaac atgagtggaa
    19981 aacgtgccag tagcggagta tatttggtat ttgtaagcga tgatcaaggg gttgaaaaaa
    20041 tggttacaaa aattttgttc atcaaataac tttttatttc aaaaaatgat ctcaaaaacg
    20101 cgaggaatca tttttaaaac aataaaatat tcggataata gcctgatagt aaaagtatat
    20161 accgaacttt ttggactgcg ctcttacatt actcgcggat taaacaaaaa gagaatcagt
    20221 ataaacaaaa gcaatctcca accgctttct attgttaata tcctagttta cgaaaatgga
    20281 aaaggaggtc ttcaaacaat taaagagata gagagcgctt atccttaccg gtcgttgccc
    20341 ttcgatgtac gcaaaagctg catagcgctg tttatcaacg aagtgatgta tcattcaata
    20401 cgtaacgaaa tagctgacat cgaactcttt aaatttctct ataataccct gattaacctt
    20461 gatgaaatta aagataagta tgagaacctt catcttcagt ttttaattaa cctcgccaaa
    20521 taccttggca ttgcacctag aaataattat agcgcgacaa atcagttttt cgaccttcag
    20581 gaaggaatct tcatcagcga gccaccactg catgctaact ggctgaacga aaccttaagc
    20641 cagaagcttt ttcaaatgat aaaacaacca gaaacaaccc ctcttttatt tactaatgca
    20701 gcagcacgta acgaaatcct tttcaatctg atgaattact tcagatttca tattccaggg
    20761 tttggcgagc taaaatcacc cgctatactt agcgaggtgt taacctaaat tttttaaaat
    20821 gattaacaaa atagctcgca attttttata cttagcttta ctattcatt
//
LOCUS       DAOOAI010000029        20852 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_3358_length_20852_cov_5.217435, whole genome shotgun sequence.
ACCESSION   DAOOAI010000029 DAOOAI010000000
VERSION     DAOOAI010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20852)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20852)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20852
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3358_length_20852_cov_5.217435"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /locus_tag="PK345_03445"
     CDS             <1..332
                     /locus_tag="PK345_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444160.1"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPX34107.1"
                     /translation="TTLRGFDLNRQRRISFEYIMFKDLNDTQRHVNELARILNGIRCR
                     INLIRFHPIPNTTLRGLDERTIEKFMIALNKKGIRTTIRASRGQDIWAACGLLSTKEL
                     VNKTQDN"
     gene            578..1648
                     /gene="serC"
                     /locus_tag="PK345_03450"
     CDS             578..1648
                     /gene="serC"
                     /locus_tag="PK345_03450"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HPX34108.1"
                     /translation="MKKHNFYAGPSILPQFTLDETVSAIRDFAGTGLSLLEISHRSKQ
                     FVAVMDEATAMFKELLNIPEGYSVIFLGGGASLQFCMVPFNLMNKKAGFLVTGEWATK
                     AHKEAKNFGEAVEVASSKDKNFSYIPKNFNVPKDLDYFHITTNNTIYGTQLRKDLDVG
                     VPLVADMSSDIFCRPLDVSKYALIYGGAQKNLAPAGVTFVIIRNDILGKVDRKIPTML
                     NYQTHIDKGSMFNTPPVMPVFSALQTLKWLKAQGGVAAMEKINKHKAELLYAEIDRNP
                     YFKGTVEKEDRSWMNICFVMSDEHKALEQEFLDFATSKGMVGIKGHRSVGGFRASTYN
                     ALPLESVKALIDTMKEFEKMKS"
     gene            1666..2589
                     /locus_tag="PK345_03455"
     CDS             1666..2589
                     /locus_tag="PK345_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005855649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HPX34109.1"
                     /translation="MKKVLVATEKPFAAKAVKAIREVVENAGYELVLLEKYTDKEQLI
                     SAVANVDAMIIRSDIADKAVLDAAKNLKIIVRAGAGYDNIDLAAATANKVVAMNTPGQ
                     NSNAVAELAIGMMIYMARNKYDGKAGTELREKTLGIHAFGNVGKLIGSIAKGIGMEVY
                     AFDPFIKKEDIIAGGATPVDSVEELYKKCQYISLNIPATDQTKKSIGYKILSTMPENA
                     VLVNTARKEVIDEEGLLKMFELRPDFKYISDIAPDCIADIESKYPNRYFFTPKKMGAQ
                     TSEANINAGIAAARQIVDFFKKGVTTFQVNK"
     gene            2690..3088
                     /locus_tag="PK345_03460"
     CDS             2690..3088
                     /locus_tag="PK345_03460"
                     /inference="COORDINATES: protein motif:HMM:TIGR02436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HPX34110.1"
                     /translation="MENQNNTQTFFRFEDLRVYHKALNYIEWVHRTICLLPEIENQSL
                     STNFINSAQVIAIQIAEGSSRNKTQFANHLKTAKSAIRECVVYTAIAKNLNYISEDHF
                     NESRNLLMELTKMVAALIGSLQRGDTTNQE"
     gene            3118..4371
                     /locus_tag="PK345_03465"
     CDS             3118..4371
                     /locus_tag="PK345_03465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 family protein"
                     /protein_id="HPX34111.1"
                     /translation="MAILKAFKGLRPPKEIARDLASRPYDVLNSEEARKEAYGNKYSL
                     LHIIKPEIDLPEDIDLYAPEVYKKAKENFELFQKNGWLVPDDDEYLYIYAQTMNNKTQ
                     YGLVGCAAVEDYMNNRIKKHELTRKAKEEDRMKHVRITNANMEPVFFTYPAIPKIDSI
                     VADWVKNHEPIYDFIAVDGFGHHFWVVDDKKTIELLINLFADLPVTYVADGHHRTAAA
                     ALVGNEKRMNNPNHKGDEEYNYFLAVHFPDNQLTIIDYNRVVTDLNGMTKQEFINNLK
                     VHFDIVEKGENIYKPDRLHTFGMYIDKKWYSLTAKPGTYNDSDPIGVLDVTILSNLVF
                     DQMLNIKDLRTSERIDFVGGIRGLEELERRVDSGEMAAAFALYPVSMQQLINIADTNN
                     IMPPKTTWFEPKLRSGLVVHLLDKI"
     gene            4383..4559
                     /locus_tag="PK345_03470"
     CDS             4383..4559
                     /locus_tag="PK345_03470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004319923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HPX34112.1"
                     /translation="MEIKDIVLKTLKESTEPLKGGEIAEKSGVDKKEVDKAIKILVKE
                     NLIHSPKRCFYAAK"
     gene            4675..5442
                     /locus_tag="PK345_03475"
     CDS             4675..5442
                     /locus_tag="PK345_03475"
                     /inference="COORDINATES: protein motif:HMM:NF016955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerB family tellurite resistance protein"
                     /protein_id="HPX34113.1"
                     /translation="MANYGKWIGGGIGWVLGGPIGALLGYLFGSMFDSLSKGEFEYYQ
                     PTAKDFTGQGHKSNTPQTQHGDFTISLLILTAAVMRADKRVLKSELNYVKSFLISQFG
                     TVQAAQFVLMLRDILKQDIDTQGVSKQIARYMDYSSKLQLLHYLFGIVLADGHSHSEE
                     ILVVETIANYLGISSADYTSIRAMFIKDNQNNYAILNISPQASDEEVKKAYRRMTLKH
                     HPDKVAHLGSDIQEAAKEKFQKINEAYSAIKAERGMT"
     gene            complement(5707..7764)
                     /locus_tag="PK345_03480"
     CDS             complement(5707..7764)
                     /locus_tag="PK345_03480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34114.1"
                     /translation="MKHYIYYLFLSIIFVLAGCGEKGMKKVDLSETKLNDEIIQDVVS
                     ELLKNHGEAFRFRIERGVGQVASLWRVSDGNESEFNVFCQEYFIADEDELDKLFQRLL
                     LNYEILGGNMLRMKKDLMRPLHLDLGPILPVDMLFGSYEPGAHMQEDFFENKIAFVVM
                     LNFPYYSLDEKTQLSDKWSRREWAMARIGDYYTSRVPATLIQNYVAINTAADAYIDNY
                     NIYMGNLIDTNGEKLFPADLRLISHWGLRDELKSNYADENGIEKQKMVYAVMKRIIDQ
                     TIPEKVINSDSYDWNPFTNQLFDNSESVDFQSEPDRRYEHMLNNFKAQYQLDPYYPQT
                     PNYIQRQFEANMEIPQQDVEKLFVEFISSPTVNKVAEYIRSQLGRQLEPFDIWFNGFK
                     AGSKYSQEQLDRITMTRYPNVEEVQNDLPRMLRTLGFSPEKADFIASQIKVEGSRGAG
                     HAWGALTRDDKALLRTRIGANGMDYKGYNIAVHEFGHNVEQTISLHDVDYYSLNRVPN
                     TSFTEALAFIFQKRDLELLGLSGGDPMKEHMNALANFWSCYEIMGVSLVDMEVWKWMY
                     ANPNAMPEELKEAVITIAKDIWNKYYTPVFGQKDEPILAIYSHMISNPLYLSNYPLGH
                     LIDFQIEQYIADKNFADEILRIYKLGSIIPQQWMKEAVGQPLSTQPTIEAAEMALEVI
                     GNN"
     gene            8079..8510
                     /gene="dut"
                     /locus_tag="PK345_03485"
     CDS             8079..8510
                     /gene="dut"
                     /locus_tag="PK345_03485"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010956168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HPX34115.1"
                     /translation="MEIKIVNRSGYSLPAYETEASSGMDIRAKISSPITLKSLERALV
                     PTGLFVEIPLGYEAQIRPRSGLAAKHGISLVNTPGTIDADYRGEIKVIMINLSNENYT
                     IHDGDRIAQMVISKVEHIHWKVVDELEQTTRGEGGFGHTGK"
     gene            8628..10388
                     /locus_tag="PK345_03490"
     CDS             8628..10388
                     /locus_tag="PK345_03490"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX34116.1"
                     /translation="MIKQQKTIKSKTLVFSVFLLSFLVSCNFCIAQSKSKKSTKLSEF
                     QINQRKEIFLNAVKERTLGNYNEAEKLYKDVLAIDPEHDASMYELARIYLTKNRSEDA
                     VKLLEKACELSPDNEWYLELLAGVYKETYQIDKLIIAQEQLVKRNPGKIDYKLNLSLI
                     YVINGDIKKAIAVLDEIEEQIGKTEEVSIQKKQLYLISKKPKQATDELESLVNAYPDN
                     TRYLQILAETYLDTDQEDKALTIYKRIKEIDPDNPYIHISMADLYKKQGNDNLAIEEL
                     KAGFANTALDVDTKIHVLLSFFSFDEYYNTKKSVVLELVTILAETHPDNNTALAVYGE
                     MLNHNQEHEKALEVFDRIIANDESNYSWWEQKLFLENQLQKSDNVYNTSQRMIELFPM
                     QPLPYLFNGFALLEKKNYEAARHSLENGVMLVIDNDLLEGQFYSTLGDVYNKLKEHEK
                     SDRSYDKALKLKPDDAYVLNNYSYHLTLRMENLPKAKEMAAKANELMPDKASFQDTYG
                     WVLFKMGDYENAEKWLKKAIDNDNKNSPVLLEHYGDVLFKLGRKTDAMKYWIKAKENT
                     KEGETTESLQEKIRTGILHE"
     gene            10381..11229
                     /locus_tag="PK345_03495"
     CDS             10381..11229
                     /locus_tag="PK345_03495"
                     /inference="COORDINATES: protein motif:HMM:NF025490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4292 domain-containing protein"
                     /protein_id="HPX34117.1"
                     /translation="MNKIATKKISFIPRKIYLIIAFAAFIILLNSCKGYRSTLKAPIK
                     EQGADYLMEQLQLNSFKFNTLTSKFTVDYAYKRIRYDIKGQIRIVKDSAIWVSFNQDL
                     GMELARLLITQDSVKFMNRLTKSYFAGNHSFVNDFLNANIDYEILQSLILGNDFEYYE
                     NASFKAAIDGKNYKLSTTGRSKLKKHVRNNDDNTRILLQTIWLNPNTFKINEIKLKEL
                     TKDSKKLNAKYSNFVNIGSQKLASRADYVIEDKSPIKVTVKHSKIITDEKVSMPFAIP
                     DNYRRE"
     gene            11278..12804
                     /locus_tag="PK345_03500"
     CDS             11278..12804
                     /locus_tag="PK345_03500"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013759858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase"
                     /protein_id="HPX34118.1"
                     /translation="MTNNEDKLKKIISHSKEYGFVFPSSEIYDGLSAVYDYGQYGVEL
                     KNNIKDYWWKSIVQFHENIVGLDSSIFMHPTIWKASGHVDAFNDPMIDNKDSKKRYRA
                     DVLVEDHILKIESKIEKEIEKAAKRFGDSFDKEQFVTTNLRVTGYLEQIAEIRKKLAA
                     AMDNDDLSDLRRLIEELEIVCPISGSRNWTDVRQFNLMFSTSIGSLSEGANTIYLRPE
                     TAQGIFVNFLNVLKSGRMKVPFGIAQIGKAFRNEIVARQFIFRMREFEQMEMQYFVRP
                     GTELDWFKYWKDQRLKWHLGMGIPEEYYRFHEHDKLAHYANAAFDIEFNFPMGFKELE
                     GIHSRTDFDLEAHQQYSGKKIQYFDPELQENYVPYVVETSIGLDRTFLAVLSYGYHEE
                     TLDDGSERVVMRIPPFLAPIKAAVLPLVKKDGMPEKAREIFDWLKMDYMIQYDDKDAI
                     GRRYRRQDAIGTPYCITIDSETLENDTVTIRERDSMKQDRIPINKISTIINERLTLKS
                     "
     gene            complement(12860..13468)
                     /locus_tag="PK345_03505"
     CDS             complement(12860..13468)
                     /locus_tag="PK345_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPX34119.1"
                     /translation="MKDTQYKLLNTGAFLFAGIVFLVMIFQYFVGPKYKVSSAEMLEQ
                     VENQQRWVMPADLLSLVESNRLQDYLLVDLRNEEEYNRGTMPGAVNISFDKLLDSESL
                     KQLRGRKDIILFSGTESQASLASLLLAGKGFKNVLVMANDFSFIKESVLEKYKSSNAF
                     THSEKAQYDYPRFFKTSPIEAKQSNASKPKIIETKMIVSGGC"
     gene            complement(13488..14714)
                     /locus_tag="PK345_03510"
     CDS             complement(13488..14714)
                     /locus_tag="PK345_03510"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPX34120.1"
                     /translation="MGPLIPQGFINPDLNLFFAFVIGLGFGSVLEQAGFSSSRKLAGL
                     FYGYDFIVLRVFFTAAITAMTGLLLFSYLGWIDYSMLYVNPTYFWSAIVGGVIMGLGF
                     IMGGFCPGTSVVGAVIGKIDALFFFFGMFIGIFLFGHFYDSFESIYLGHNLGNIFVFD
                     SLGMSREWFAFMLILVALIAFLITQRIEDIVNDTPATVRQSRPSHFFPSFILIFSGLL
                     LLLLPSQPRNSWYETDAKTILSEVANKNHYTDADEIAFSIMQGKNYTVLLVDVRDPES
                     FQRFTLPGAINIPLNDIFDYNWESIFDNHNQKVVLFSHSETQASEAWLLLRRKGYKEM
                     KVLEGGLNNFMNTIFIEEFIPERKRPDCIDMHTARFRTEAKEYFTTGKAIKKIESPQI
                     PVIKIVEIEKPISGGC"
     gene            complement(14729..15226)
                     /locus_tag="PK345_03515"
     CDS             complement(14729..15226)
                     /locus_tag="PK345_03515"
                     /inference="COORDINATES: protein motif:HMM:NF016064.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HPX34121.1"
                     /translation="MNSYLAGFLLGLVLLSAFVVTGRGLGASGAVKSVVISSVASVAP
                     EYAKAHPFYDDFFNEGPAPMKNWLVYLTLGVIAGSFFSGAMSGRLGFKVERGPRISST
                     TRLIFATLGGLFFGLGSQFGRGCTSGTALTGMAILSTAGFLTMMAIFGAGYVFAYFFR
                     KLWLS"
     gene            complement(15344..16429)
                     /locus_tag="PK345_03520"
     CDS             complement(15344..16429)
                     /locus_tag="PK345_03520"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HPX34122.1"
                     /translation="MQHHFIQKSLFLISVCFSLLVLLSCSEQKVKDKPQDQTSDKTLS
                     LAKWFEANGNYINSDEVPSIVDAQLVYALRDENIMVIDLRPTEEFNDGHIEHAINIQP
                     SQVLDFFKNDVEPSSFKGIFFVCNDMSISGYVTGIMRLLGYDNVYAIRYGMSGWSREV
                     AEKFWLENISNLLLGKLETKGYPRNEPGELPVIDAERDSEYDIALEQAEKMLQNTESI
                     NITIAELLKNPEAYYIICYWPEDKYFNNGHLPGAVQYDPRKSFGLNMYLNTLPVDKPV
                     VVYCYAAQHSIFVVAYLRMLGYDAKSLNYGANAFIHEVIAKTEPRPTRTFTENLIQDF
                     PIVRSGFVEPEKPILIKKEEIKIVGGC"
     gene            complement(16457..17098)
                     /locus_tag="PK345_03525"
     CDS             complement(16457..17098)
                     /locus_tag="PK345_03525"
                     /inference="COORDINATES: protein motif:HMM:NF013460.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HPX34123.1"
                     /translation="MKNTKKVYVYKGFERFWHWTQALLIMFLALTGFEIHSSYSLFGF
                     ETAVNLHNKAAWGFIVLIVFAIFWHITTGEWKQYIPTTKNLKAQIEFYITGIFRNAPH
                     PTKKTLLSKLNPLQRLVYLGLKILIIPVMVVSGLLFMFFRYPQSGMIKTVNIENLEIV
                     ALIHTLGAFLLITFLIVHLYLITTGHTITSNLKAMITGWEELEEDTEETKQEN"
     gene            complement(17115..18701)
                     /locus_tag="PK345_03530"
     CDS             complement(17115..18701)
                     /locus_tag="PK345_03530"
                     /inference="COORDINATES: protein motif:HMM:TIGR04315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetrathionate reductase family octaheme c-type
                     cytochrome"
                     /protein_id="HPX34124.1"
                     /translation="MQNLRTVITFLLAIVAPLVIITIILRETPADHTLLNNLRETYEI
                     EHKPGVDHSKHEVLQQEFTNPHLITAACLSCHTERGQEVMKNSHWKWEREAYIPGKGV
                     TYLGKKTLINNFCTGISSNEGTCNRCHIGYGWQDKTFDFTNEYNIDCLICHDNTGTYE
                     KARGAAGYPPMGQDAPDYKNILAHVGRPKRNNCGYCHFHGGGGNNVKHGDLELALLTA
                     DRTIDVHMGVDGMNMACVTCHTAENHQIQGRYYGVSSSNTQRVTCEQCHTSVPHTNSK
                     INEHTIKVACQTCHIPVYAKVNATKTAWDWSTATQRKDGLPYELLDTSGNETYTSIKG
                     DFHWERMVKPEYIWFNGTADHHMITEKINLDNLPLNINTLNGEYRDRNSKIYPTKIHR
                     GKQPYDPVYLTIIQAKLWDKEVNKGALWVDLDWEAALRTGMEYLDLPYSGTYDFIPTQ
                     LFLPVNHMVSLASESVSCSECHTRKDGRLNDLRDFYMPGRDYHVGIDTFGKLMIIFSF
                     IGVLIHGTIRVFASRKNQIK"
     gene            complement(18823..19566)
                     /locus_tag="PK345_03535"
     CDS             complement(18823..19566)
                     /locus_tag="PK345_03535"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPX34125.1"
                     /translation="MISFRSHILVLILSLFYVNLFGQGKTLTFSLQVEPLIPSDLIRS
                     NNVKAYVDAVEFSSDPLRGIGYGAGVSYHFHPKLALQTGINYLKRDSKITATELNDFT
                     LELRYTVDNFEIPLTANYFVRLGEKVYMDGSVGFSFQFLPGELKSRKSLQNDIGGYIY
                     DFSQISWRNYWVMPVFRGGFGFEYRTEKSGSFYIGPVFRLFSTLYYTQILYMHNDINI
                     SNLVIKPAGDYFSIAFKYIFPHETPKKEK"
     gene            complement(19566..>20852)
                     /locus_tag="PK345_03540"
     CDS             complement(19566..>20852)
                     /locus_tag="PK345_03540"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPX34126.1"
                     /translation="KRNKLTNGLELLYIENVENPTFSMSYIFPYGSNHDRLLPLAASF
                     LDFMGTGKMSAEEISNEFYKLACNFSIRVTDEETRIILSGLSENLTEAVGLFENLVAN
                     ATAKISDLELYVKNVKKSRLDSKANQRANFQALVNYATYGEKNPTTFTLTNDELDKLT
                     IADLASSLQKIWQLEHSIAFYAPQSLDELTKLTEIHHKAPKRLLPVDKASRFEPLDTD
                     KEIIYFAHYDANQSYLQTITKGIPYSKELLPEINLYNAYFGGGMNAIVFQEMREKRGL
                     AYSSSAAYRSPSTPEGVFMNVSFIATQNDKVIEAFEAFNDLFNNIPLSKNAFQLAKEQ
                     IITDIQTERITKSGIIYTYLNDKKFGFDTDRRKLFYSQTPKMSLEEMKAFNEKYIKEK
                     PKTYVILGNRDQMNFDELEKRFGKVIKLTESDLFNY"
BASE COUNT         6857 a         4089 c         3827 g         6079 t
ORIGIN      
        1 ttaccacact tcgaggcttt gatctcaatc ggcaacggcg tatatcattc gaatatataa
       61 tgttcaagga ccttaacgat acacagcgcc atgtaaatga gcttgctcgc atcctaaacg
      121 gtatccgctg ccgtataaat ctcattcgtt ttcatccaat acccaacaca acacttcgtg
      181 ggttagatga gcgtactatt gaaaaattta tgattgcact taacaaaaaa gggatacgta
      241 caacaatccg tgcttcccgc ggtcaagata tttgggcagc ctgcggatta ttatctacta
      301 aagagttagt gaacaagaca caagacaatt gattaattca ggataaactc aggttttata
      361 aatcgaaata atctaccccc tttcgtgaaa acaattttcg ataaaataaa gatttcgcag
      421 acttaattaa atcaaaaaaa acaggttgca acagtttagg aattaattta atcagaacta
      481 ttccatttta ttcattaata ggcaaaaaat ccgttaaatt tctataattt tgcggcaaat
      541 ttttaaacca aatatcaaat aatttaattt ttttgacatg aagaaacata atttttatgc
      601 tgggccctct atcctcccgc aatttacatt ggatgagaca gtaagcgcaa tcagggattt
      661 tgccggaacc ggattgtcgt tgcttgagat ctcacatcgt agtaaacaat ttgtggcagt
      721 catggacgaa gcgactgcta tgttcaaaga gcttcttaac atccccgaag gctattcggt
      781 aattttcctt ggcggtggag ctagcttgca attttgcatg gttccattca acctgatgaa
      841 taaaaaggct ggcttcctcg ttactggcga atgggcaacc aaagctcata aagaggctaa
      901 gaattttgga gaagcagtag aagtagcatc ttcaaaagac aaaaatttct cctatatccc
      961 taagaatttt aacgttccaa aggatcttga ttatttccat atcacaacta ataacacgat
     1021 ttatggtaca cagttaagga aagaccttga cgttggcgta cctttggttg ccgatatgtc
     1081 atccgacatt ttctgccgcc cattggatgt ttcaaaatat gctcttattt atggtggtgc
     1141 acagaaaaac cttgcccctg ccggtgttac attcgttatt atccgcaacg acattctggg
     1201 taaggtagat cgtaaaatac ctacaatgct caactatcag acacacattg acaaaggatc
     1261 aatgtttaac actcctccgg ttatgcccgt tttctcggca ttgcaaacac taaaatggct
     1321 gaaagcacaa ggcggtgttg ctgcaatgga aaaaataaat aagcacaaag ccgaactatt
     1381 atatgccgaa attgatcgta atccctattt caaaggcaca gtggaaaaag aggaccgctc
     1441 atggatgaat atctgttttg taatgagcga tgaacataaa gctcttgaac aggaatttct
     1501 tgactttgct acttcaaagg gtatggtcgg aataaaagga caccgttctg ttggtggatt
     1561 cagggcttca acctataacg cactcccact cgaaagcgtt aaagcgctaa tagatacgat
     1621 gaaggaattt gaaaaaatga aatcttaacc ttaaaattat taataatgaa aaaagtatta
     1681 gttgctaccg aaaaaccatt tgccgctaag gctgttaaag cgataagaga agttgttgaa
     1741 aacgctggtt acgaacttgt tttacttgaa aaatataccg ataaggagca gcttatcagc
     1801 gctgttgcca atgtagatgc aatgattatc cgtagcgata ttgccgataa agctgttctg
     1861 gatgccgcta aaaacctgaa aataattgta agagcaggcg ccggttacga taatattgac
     1921 cttgcagccg ccactgccaa caaagtcgtt gctatgaaca cacctggtca aaattcaaat
     1981 gccgttgccg aactggcaat aggcatgatg atatacatgg cccggaataa atatgacggc
     2041 aaggcaggaa ccgagctacg cgaaaagaca cttggaatcc atgcatttgg aaatgtaggc
     2101 aaacttattg gctcaattgc gaaaggaata ggtatggaag tttatgcttt cgatccattt
     2161 attaaaaaag aagatataat tgctggaggc gcaactcctg ttgatagcgt agaagaactt
     2221 tataaaaagt gccaatacat ttctttaaat attccagcga cagatcaaac aaagaaatca
     2281 ataggctata agattctctc tacaatgccc gaaaatgccg tcctggttaa tacagcccgt
     2341 aaagaggtaa ttgatgaaga aggattgctc aaaatgtttg aattaagacc tgattttaaa
     2401 tatatctccg atattgctcc cgactgcatc gcagatatcg aaagcaaata tccaaacagg
     2461 tatttcttca ctccaaaaaa aatgggcgct caaacttcag aagctaacat caatgccggt
     2521 attgctgccg ccagacaaat tgttgatttc ttcaaaaaag gagttacaac ttttcaagtg
     2581 aacaaataaa ccaatgattt aatagatttg tagtttaaca atcagacctg acacgaccta
     2641 agccaggtct gattttttga taataacagc ttcattacat tgatttcgta tggaaaacca
     2701 aaacaacact caaacttttt ttagattcga ggatctaaga gtatatcata aagctttaaa
     2761 ttacatagaa tgggtacata ggacaatttg tcttttacct gaaattgaga atcaaagcct
     2821 ttcaacaaac tttataaatt cagctcaggt tatagctatt cagatagctg aaggatcttc
     2881 acgcaataaa acgcagtttg ccaatcatct aaaaactgct aaatcagcta tccgcgaatg
     2941 tgtagtttat actgccattg ctaaaaactt aaattacatc tctgaagatc attttaacga
     3001 atcgcgaaac ctattaatgg agcttaccaa aatggttgcc gctttgatcg gctcattaca
     3061 acgcggtgat actactaatc aggaataaac tttatttaat tattaatttt ttacaaaatg
     3121 gcaattttaa aagcatttaa aggcctcagg cctccgaaag aaatagcccg cgatctggct
     3181 tcgcgtcctt acgatgttct aaattcagaa gaagccagaa aggaggcata cggtaacaaa
     3241 tattcattgt tgcatattat aaaaccagag attgatctgc ccgaagatat tgacctttat
     3301 gcccccgaag tttacaaaaa agccaaagag aattttgagc tttttcaaaa aaatggatgg
     3361 ctcgtccctg atgatgatga atacctatac atctatgctc aaactatgaa taacaaaaca
     3421 cagtatggct tggtaggatg tgcagcagta gaggattata tgaataatcg tataaaaaaa
     3481 catgaactga ccagaaaagc aaaagaggaa gacaggatga aacatgttag aattaccaat
     3541 gccaatatgg agccggtatt ctttacctat cctgccatac cgaaaattga cagtatagtt
     3601 gctgattggg ttaaaaatca tgaaccgatc tatgacttta tagcagttga tggatttgga
     3661 caccacttct gggttgttga tgataaaaaa actatagaac ttcttatcaa cctatttgct
     3721 gatttgccag ttacctatgt agccgatgga catcaccgta cggcagcagc agcattagta
     3781 ggtaatgaaa aaaggatgaa caatccaaat cataaaggcg acgaagagta taattatttt
     3841 ctggccgtcc attttcctga taaccaactc accattattg actacaaccg tgtagttacc
     3901 gacctgaatg gaatgacaaa gcaagagttt atcaacaatc taaaagtgca ttttgacata
     3961 gtagagaaag gagaaaacat ttataagcct gatcgtctgc acacctttgg aatgtatatt
     4021 gacaaaaaat ggtattcgct tacggccaaa ccaggcacat acaatgattc cgaccctatt
     4081 ggagtacttg acgttactat actttccaac ctcgtattcg atcaaatgct gaatattaaa
     4141 gatttaagaa catccgagcg tattgatttt gttggcggca tccgaggcct tgaagaactt
     4201 gaacgcaggg tggattcagg cgaaatggcc gctgcttttg cactatatcc ggtttcgatg
     4261 caacaattaa tcaatatagc cgatacaaac aacataatgc cgccaaaaac cacatggttt
     4321 gaacctaaac ttcgaagcgg ccttgtagta catttacttg ataaaatata aaattaaaat
     4381 ctatggaaat aaaagatatc gtactaaaaa ctcttaaaga gtcaactgag ccattaaaag
     4441 gtggagagat agcagaaaaa tcaggtgttg acaaaaaaga ggtggataaa gccattaaga
     4501 ttttagttaa agaaaatctt atccattctc ctaagagatg tttttatgcc gcaaaatagg
     4561 atcagctttt aataagcgat tccattaatc ataatcttga attcggtaaa ataattatat
     4621 aatcagttgt tatctgccga ctgatcaata acagataata tatttcattt aaaaatggcc
     4681 aactatggta aatggattgg tggtggtatt ggttgggtac ttggcggccc gataggagct
     4741 ctcttgggct atctgttcgg ctcgatgttc gacagtttaa gcaaaggtga atttgaatac
     4801 taccaaccca ccgcaaaaga ttttaccggt cagggacaca aaagtaacac accccaaaca
     4861 caacacgggg attttactat aagtcttttg atccttaccg ctgccgtgat gagagctgat
     4921 aaacgagttc tgaaatcaga acttaattat gttaagagct ttctcattag ccaattcggc
     4981 actgttcagg cagcccaatt cgtactgatg cttcgggata tccttaaaca ggatattgac
     5041 acacaaggtg tttccaagca aattgcaaga tatatggatt attcatcaaa actgcaatta
     5101 ttacactacc ttttcggaat agtgctggcc gacgggcatt cgcatagtga ggaaattctt
     5161 gtcgttgaaa caattgctaa ttatttagga atatcttccg ctgattatac ctctattaga
     5221 gcaatgttta ttaaggataa tcagaataat tatgcaattc tcaacataag tccccaggca
     5281 agtgatgaag aggtaaaaaa agcatacaga cgaatgactc tcaaacatca ccccgacaag
     5341 gttgcacatt tgggatcaga tattcaggaa gcggcaaaag agaaatttca aaaaattaat
     5401 gaagcataca gcgccatcaa agctgaaagg ggaatgactt aaaaaagagg taacaacaat
     5461 tacaaaccgc taagtccgtc agaaaattta cagccaccaa cggcaaaact acctgctaac
     5521 cgccaaagca gttgaatatt tcagtaaaaa tcttaaagaa caccctgacc atgaaaagtt
     5581 aactacgatt taaaaacagg gttttctata gattacttcg gttacaattg ccgataatag
     5641 taaaaccttt ttttttaaag aagcaaaata ttttttgtta acaaaaactt cagttgacaa
     5701 gcagatttaa ttattgccta tcacttccaa agccatttca gcagcttcaa tagttggctg
     5761 agttgaaagc ggctgcccaa ctgcctcctt catccattgc tgcgggataa tactaccgag
     5821 cttatagatt cgaagaatct cgtctgcgaa atttttgtcg gcaatatatt gctctatttg
     5881 aaaatctatc aggtggccaa gtggatagtt ggagagatag agcgggttgc tgatcatgtg
     5941 gctgtagatg gctaagatgg gttcatcctt ttggccaaaa acaggtgtgt aatatttgtt
     6001 ccagatatct tttgctatag ttattaccgc ctcttttaat tcttctggca tagcattagg
     6061 attggcatac atccatttcc acacctccat atctaccagc gatacaccca taatttcata
     6121 gcacgaccaa aaattagcta atgcattcat atgttctttc attgggtctc ctccgcttag
     6181 tccgagcagt tcaagatcac gtttttggaa aataaaggca agagcttcgg taaaagaggt
     6241 atttggcact cggttaagtg aataataatc tacatcgtgc aacgaaatcg tttgttcaac
     6301 attatgtccg aattcatgaa cagcgatgtt gtaaccttta taatccatgc cgttggcacc
     6361 tatccgggta cgtagcaatg ctttatcatc acgtgtaagc gctccccagg catgccctgc
     6421 tccacgcgaa ccttcaacct taatctgaga agcgataaaa tcagcctttt cagggctaaa
     6481 accaagtgtt ctcaacatcc ttggaagatc attctgaacc tcttcaacat taggataacg
     6541 agtcatagta atacggtcaa gctgctcctg agaatacttg ctacctgcct tgaaaccatt
     6601 aaaccaaata tcgaaaggct caagctggcg gcctaactga cttctgatat attctgcaac
     6661 cttatttacc gttggacttg atataaattc gacaaacaat ttttcaacat cctgttgtgg
     6721 tatttccata tttgcctcaa actggcgttg aatatagttg ggggtttgtg gatagtatgg
     6781 atcaagttga tattgtgcct tgaaattgtt gagcatgtgc tcataacgcc tatctggttc
     6841 actttgaaaa tcaacagatt cagaattatc aaaaagttgg tttgtaaatg ggttccaatc
     6901 ataagaatca ctattgatca ccttttcagg tatcgtttga tctataatcc tcttcataac
     6961 ggcatacacc attttctgct tttcaattcc attttcatcg gcataatttg atttcaactc
     7021 atcgcgtaat ccccagtgag agatcagacg aaggtcggca gggaaaagtt tttcaccatt
     7081 agtatctatc aaattgccca tatagatatt gtaattatca atgtatgcat ctgctgcggt
     7141 attgatagct acataatttt gtattaatgt ggctggcaca cgtgaagtat aataatcacc
     7201 aattctggcc attgcccatt cccgacgact ccatttatca ctcaactggg tcttttcgtc
     7261 taatgaataa tatggaaagt ttaacatgac cacaaaggcg atcttgtttt caaagaaatc
     7321 ttcttgcata tgcgctccgg gctcatagct tccaaacaac atgtcaaccg gcaatattgg
     7381 ccccaaatca agatgaagag ggcgcattaa atcctttttc atacgcaaca tgttgcctcc
     7441 caaaatttca taattcaaca aaagcctttg aaacaattta tcaagttcat cttcatccgc
     7501 aatgaagtac tcctgacaaa atacattgaa ctcactttcg ttcccatcgc taaccctcca
     7561 gagtgaagca acttgcccta ctccgcgttc aatcctgaaa cgaaaagcct cgccatgatt
     7621 ctttaataat tcactaacga catcttgaat aatttcgtcg tttagttttg tttccgataa
     7681 atctactttt ttcatacctt tctctccaca acctgccaat acaaatatga ttgacagaaa
     7741 aagataatag atgtaatgtt tcatttcaat attctgaatt taaagttaat gtacaaaggt
     7801 agtaaatatt gagaagtctt tgctttttgt aacgaatttt aacccttatt aagcatttga
     7861 caataatcaa atgtcagtga tcaacacaat aagactttta ctgagcaacc gccaaatcgg
     7921 gatctgcatc ataaagtcaa cagttcaacc caaaagggct ctctattgat taatccaggt
     7981 ttaatgaaaa atttacatat tgaaatataa cacattatag taaccgccag gaaatctaaa
     8041 tcttttggct acttttgccg ccttatttta caggagtaat ggaaattaaa atcgttaatc
     8101 gttcgggcta ctctttgccc gcttatgaaa cggaagcttc gtccggaatg gatatccgcg
     8161 ctaaaataag ttcaccgata acactgaagt cattggaaag ggcgcttgtg ccaacaggcc
     8221 tttttgtcga aataccatta ggctatgagg cacaaatacg accacgtagc ggcctggctg
     8281 ccaaacacgg aatatcgctg gtaaatactc caggaacaat tgatgctgat tatcgcggcg
     8341 aaattaaagt gatcatgata aacttatcaa acgaaaacta tactatacac gatggcgaca
     8401 gaattgcaca aatggtgata tcaaaagttg aacatattca ttggaaagta gttgatgaat
     8461 tggaacaaac aacaagagga gaaggcggat ttggacatac cgggaagtaa tcaataatta
     8521 aagacaaaaa tatttttaaa tctagaataa aattaggcta aaacttgttt attgttatcc
     8581 ggtttatgtt actggggcga acaataataa agacatagat aaaatcaatg attaaacagc
     8641 aaaaaacaat aaaatccaaa accttagttt tttcggtatt tctactttca tttcttgttt
     8701 catgcaactt ttgcattgcc caatcaaaat ctaaaaaatc cacaaagctc tctgaatttc
     8761 agattaacca acgaaaagag atttttttaa atgctgttaa agagagaacg cttggcaact
     8821 acaacgaagc agaaaagcta tataaagatg ttttggctat tgatcctgag catgacgctt
     8881 cgatgtatga acttgcgcgc atctatctta caaaaaaccg gtcggaggat gctgtaaagt
     8941 tgcttgaaaa ggcctgtgaa ctaagccccg ataatgaatg gtatctcgag ctattagccg
     9001 gagtatataa agagacatat caaattgata agttaataat agcacaggaa caactggtta
     9061 aaaggaaccc agggaaaatt gattataaac tcaatctttc cttgatttat gtcattaatg
     9121 gcgatataaa aaaagcaata gctgttttag acgaaattga ggaacaaata ggtaaaactg
     9181 aagaggtttc gatccaaaag aaacagttgt acctcatatc caaaaaacct aaacaagcga
     9241 ctgacgaatt agaatcctta gtaaacgcct atcccgacaa tactcgatat cttcaaattt
     9301 tggcagagac ttacctggac actgatcagg aagacaaagc attaactatt tacaaacgga
     9361 ttaaagagat agatcccgac aatccttaca tacatatttc gatggcggat ctatacaaaa
     9421 aacagggtaa tgataatctt gccatagagg agttaaaagc cggatttgct aacacagcgc
     9481 ttgatgttga cacaaaaata catgttttgc tatcgttctt cagcttcgac gaatattata
     9541 atacaaaaaa gagtgtcgtt ttggagttag ttacaatact ggccgaaaca catcccgaca
     9601 ataacacagc attggcagtg tatggcgaga tgcttaatca taatcaagaa cacgaaaaag
     9661 cattagaggt ctttgaccga ataatagcta acgacgagag caactatagt tggtgggagc
     9721 agaagctttt tctcgaaaac caactccaaa agtcggataa tgtatataat acaagccaac
     9781 gcatgataga actttttcct atgcagccac tgccctatct ctttaatggc tttgcattgc
     9841 tcgagaaaaa aaactacgag gctgcacgtc attcgctcga aaatggggta atgctggtta
     9901 ttgacaatga tctactcgaa gggcagtttt atagcacttt gggtgatgtt tacaacaaat
     9961 tgaaagaaca tgaaaaatct gacagaagct atgacaaagc attaaaactt aaacctgatg
    10021 atgcttatgt actcaacaat tatagttacc acctcaccct gcgtatggaa aatttgccaa
    10081 aggctaaaga gatggccgca aaagccaatg agttgatgcc cgacaaagct tcgtttcaag
    10141 atacttatgg ctgggtactt tttaaaatgg gagattatga gaatgcagaa aaatggttaa
    10201 aaaaagctat tgataatgat aataaaaata gccccgttct tctggaacat tacggcgatg
    10261 tgctttttaa gctcggacgt aaaaccgatg ccatgaaata ttggataaaa gcaaaagaaa
    10321 acactaaaga gggcgaaaca acagaatctt tacaggaaaa aataagaaca ggcattctac
    10381 atgaataaga tcgctacaaa gaagatttca tttatcccac gaaagattta cctgattatc
    10441 gcttttgcag cctttatcat cttacttaac tcctgcaaag gctacagaag tacattaaaa
    10501 gctccaataa aagaacaggg cgctgactac ctcatggaac agctccaatt gaatagtttt
    10561 aaatttaata ccctaacctc aaaatttacc gtagattacg cgtataaaag gattcgctat
    10621 gatattaaag gccagattcg tatagtaaaa gatagcgcca tttgggttag ttttaatcag
    10681 gatttaggta tggagttagc tcgattgcta ataacacagg attctgtaaa attcatgaat
    10741 aggctaacta aaagctattt tgccggaaat catagttttg taaatgattt tttgaatgcc
    10801 aatattgact acgaaatcct acaatcgtta attcttggaa acgactttga atattatgaa
    10861 aatgccagct ttaaggccgc tattgatggg aaaaattaca agctgtcaac tacaggccga
    10921 agcaaactaa aaaaacatgt ccgcaataat gatgacaaca cgcgaatttt gctgcaaaca
    10981 atatggctca atcctaatac atttaaaata aacgagataa agttaaaaga actgacaaag
    11041 gatagtaaaa aactcaatgc aaagtattct aattttgtta atatagggtc tcaaaaatta
    11101 gcatcaagag ccgattacgt tattgaagac aaatcgccaa taaaggttac agtgaagcac
    11161 tcaaaaatta ttactgatga aaaagtttct atgccttttg caattcctga caactacagg
    11221 agagaataaa acaacattaa tataattacg aataaagaat cttttctaaa atactttatg
    11281 acaaataacg aagacaaact caaaaaaatc atatctcact ccaaagagta tggatttgtt
    11341 tttccgtcaa gtgaaatata cgatgggctt agcgctgttt atgattatgg tcagtatggt
    11401 gtcgagctta aaaacaacat aaaggattac tggtggaaat caattgtaca gtttcacgaa
    11461 aacattgtag gcctcgattc ttccatattt atgcatccta ctatttggaa agcttcaggc
    11521 catgtggatg cttttaatga cccgatgatt gacaacaagg attccaaaaa acgctatcgg
    11581 gctgatgtac ttgtcgaaga tcacatacta aaaattgaat ctaagataga gaaagagatt
    11641 gaaaaggctg ccaaacggtt tggcgacagc ttcgacaaag agcaatttgt aaccacaaat
    11701 ctgcgcgtaa caggctattt ggaacaaata gctgaaatac gtaaaaaatt agcagcagca
    11761 atggataatg atgatctctc tgatctccgc cggttaattg aggagcttga aatagtatgc
    11821 ccgatatcag ggtcgaggaa ctggactgac gttcggcagt ttaatcttat gttttcaaca
    11881 agtataggat cactctccga aggggcaaac acaatctatc ttcggcctga aacagctcaa
    11941 ggtatttttg tcaatttcct gaacgtgctc aaatcgggca gaatgaaggt gccatttggc
    12001 atagcacaaa tcggcaaagc atttcgcaac gagattgtag cgcgtcagtt tatctttcgc
    12061 atgcgcgaat ttgaacagat ggaaatgcag tattttgtca gaccgggcac cgagttggat
    12121 tggtttaaat attggaaaga tcagcgtttg aagtggcacc ttggaatggg catcccggag
    12181 gaatattatc gttttcatga acacgacaaa ttagcccact atgcaaatgc tgctttcgat
    12241 atcgagttca acttccctat gggatttaag gagttagaag gaattcattc ccgaacagat
    12301 tttgatcttg aagcacatca acaatattca ggaaaaaaaa ttcaatactt cgatccagag
    12361 ctacaagaaa attatgttcc ttacgtagta gagacctcaa taggccttga ccggacattc
    12421 ctcgccgtgc tctcctacgg ctatcatgaa gaaactctag acgacggatc ggaacgcgtg
    12481 gtaatgcgaa tacctccgtt tctggcgcca attaaagctg cagtattacc attagtaaaa
    12541 aaagatggaa tgcccgaaaa agcaagagaa atatttgatt ggctaaaaat ggattatatg
    12601 attcagtatg acgacaaaga cgctatcggt cgccgctatc gccgacagga cgctatcggt
    12661 acaccatatt gcatcacaat tgatagcgaa acactcgaaa acgatacggt aactatacgt
    12721 gagcgcgatt caatgaaaca agatcgaatt ccgataaata aaatttcgac tattataaat
    12781 gaaaggttaa cattgaaaag ctaattgatg ctgacaatta aaattagaat ttaatcccaa
    12841 attagtcaac caataacgtt taacaacctc ctgaaactat cattttcgtt tcaataattt
    12901 ttggctttga agcatttgat tgctttgcct caataggcga tgttttgaaa aacctgggat
    12961 aatcatattg tgctttttcg ctatgtgtaa atgcattaga tgacttgtac ttctcaagta
    13021 cgctttcttt aataaagcta aaatcattag ccattaccag cacattttta aaacctttgc
    13081 cagccaataa tagactagcc aacgaggctt gcgactccgt tccggaaaaa agaattatat
    13141 cctttctacc tctcaactgt ttcaacgatt cggaatccaa caatttgtca aatgaaatat
    13201 ttacagcgcc tggcatagtg ccacgattgt actcctcctc attgcgaagg tcaaccaaaa
    13261 gatagtcctg taaacgattt gattcgacca aggacaacaa atcggcgggc atgacccatc
    13321 tttgctgatt ttcaacctgt tcgagcatct ctgcactact gactttatac tttggtccta
    13381 cgaaatattg aaaaatcatg accaaaaaaa caatccctgc aaaaagaaaa gctccggtat
    13441 ttaataactt atattgtgta tctttcataa ctttaaattt taagagttta acatccgccg
    13501 cttattggtt tttcgatctc cacaatttta ataaccggga tttgtggcga ttcaatcttt
    13561 tttattgcct tgcccgtggt aaagtactct tttgcctcag ttctgaatcg ggcggtatgc
    13621 atgtctatgc aatcgggtcg cttcctttca ggaataaact cttcgataaa aatcgtgttc
    13681 ataaaattat tcaaaccacc ctctaacact ttcatctctt tataaccctt ccttcgcaac
    13741 aacaaccaag cctcagaagc ttgagtttcg gaatgagaaa ataacacaac cttctgatta
    13801 tgattgtcaa aaatagattc ccaattatag tcaaaaatat catttaaagg tatgttgata
    13861 gcccctggta atgtaaaacg ctgaaaactt tccggatccc ttacatccac aagtaacacc
    13921 gtataattct tcccctgcat aatgctgaaa gctatttcat cagcatcagt ataatgattt
    13981 ttattggcta cttcgctaag aatcgttttt gcatctgttt cgtaccatga gttacgtggc
    14041 tgtgaaggaa gcaatagaag gagtaggccg gaaaaaataa gaatgaaaga aggaaagaaa
    14101 tgcgaaggcc tcgattgccg gacagttgcc ggtgtgtcat taactatatc ctctatccgt
    14161 tgggtaatca gaaacgcaat caaagcaact aaaatcaaca taaatgcaaa ccattcccta
    14221 ctcataccaa gcgaatcgaa aacaaatata ttacctaaat tatggccaag gtaaatgctt
    14281 tcaaagctat cataaaaatg accgaacaag aatattccta taaacattcc aaagaaaaaa
    14341 aacagagcgt caatttttcc aataacagct cctaccacac ttgtaccagg gcaaaaaccc
    14401 cccataataa atcccaaacc cattataact ccgccaacga tcgccgacca gaaatatgtt
    14461 ggattaacgt acaacatgct ataatcaatc catcccagat agctgaacag caacaatccg
    14521 gtcatggcag taatagctgc tgtaaaaaac acacgtaata ctatgaagtc atatccataa
    14581 aacaagcctg ccagcttgcg agaagatgaa aatccagcct gctcaagcac agacccaaag
    14641 ccaagcccga tgacaaaggc aaaaaagaga ttcaggtctg gatttataaa gccttgagga
    14701 ataagtggtc ccatagttta tctcctttct aacttaacca aagttttcta aaaaaatagg
    14761 cgaatacata tcctgctcca aaaatggcca tcatggttaa aaaaccggct gttgatagga
    14821 ttgccattcc agtaagggca gtcccgctgg tgcatcctct tccaaactgt gaacctaatc
    14881 cgaaaaataa cccgccaagc gttgcaaata ttaatcgtgt cgttgaactg atccttgggc
    14941 ctctctctac tttaaagccc aaacgccccg acattgctcc tgagaaaaag gaaccggcaa
    15001 tcacgccaag cgtcaggtaa acgagccagt tcttcattgg ggcagggcct tcattaaaaa
    15061 aatcatcata aaaaggatgt gccttcgcat actcgggagc aacgcttgcc accgacgata
    15121 tcacaacgct ttttacagcg ccgcttgccc ctaacccacg gccggtaaca acaaaagcag
    15181 acagcaatac caagcctaat agaaaaccag ctaggtaaga gttcatgtaa gaagttggtt
    15241 ttgtcttcat atcagtatct ttattctaaa ttctgtgtaa aatattatta ttaatgtttt
    15301 atcatcttgt tgtctgtgtt taactcaaaa tcatccttct caatcaacaa ccgccgacaa
    15361 ttttgatctc ttctttctta atcaatattg gcttttcggg ttcaacaaat cctgagcgta
    15421 ctattggaaa atcctgaatc agattttcag taaaagttcg agtaggcctt ggttctgttt
    15481 tagcaatcac ctcatgtata aaagcattag cgccatagtt caacgatttt gcatcgtatc
    15541 ccaacatacg caaataagca acaacgaaaa tactatgctg ggctgcataa caatagacaa
    15601 caacaggttt atcaacagga agtgtattta aatacatgtt aagcccaaat gacttccttg
    15661 gatcatactg aacagcgcca ggaagatgcc cattattaaa atatttatct tctggccaat
    15721 agcagataat ataatatgct tcaggatttt tcaaaagctc tgcaattgtt atgtttatgg
    15781 attctgtatt ttgcagcatt ttctcggcct gctcgagcgc tatatcatat tctgaatctc
    15841 gttcggcatc tattacagga agttcaccag gttcatttct gggataacct ttggtttcta
    15901 atttacccag cagaaggttg ctaatatttt caagccagaa tttttcagct acttccctac
    15961 tccatccgct cattccatat cgtatagcat aaacattatc gtaccccagc aaacgcatta
    16021 tgcctgtaac ataccctgaa atgctcatat cgttacaaac aaaaaaaata cctttaaacg
    16081 agctgggttc gacatcgttt ttaaaaaaat cgagtacctg agagggttgg atattaatag
    16141 catgttcgat gtgtccatcg ttgaattctt ctgtcggcct aagatcaata accatgatat
    16201 tttcatcacg caaagcatat actaactgtg catcaacaat ggaaggcact tcatcactat
    16261 taatgtaatt gccgtttgcc tcaaaccatt ttgccaatga taaagtttta tcgcttgtct
    16321 ggtcttgagg cttgtcttta actttttgct cactgcatga tagcaaaacg agtaatgaga
    16381 aacaaacaga gattaaaaac aaagattttt ggataaaatg atgttgcata gcagttttga
    16441 gtttaatatg ccattattaa ttttcttgtt tagtttcttc ggtatcctct tccaattctt
    16501 cccatccggt aatcatggct tttaggttgg aagtaatggt atgtccggtt gtgataagat
    16561 aaagatgtac gatgagaaaa gtgatcaaca aaaaggctcc aagagtatgt attagcgcta
    16621 cgatctcaag gttttcgata tttaccgttt ttatcattcc cgattgaggg tagcggaaaa
    16681 acatgaataa aagacctgaa acaaccatta cggggattat tagtatttta agtccgagat
    16741 acaccaagcg ctgcaaaggg tttaatttgc tcaataatgt ctttttggta ggatgcggag
    16801 cgttacggaa aattcctgta atataaaatt caatttgtgc cttcagattc tttgttgttg
    16861 gtatgtattg cttccattct ccagtagtaa tatgccaaaa aatggcaaaa acgatcaaaa
    16921 cgataaaacc ccatgctgcc ttattatgta gatttacagc cgtttcgaaa ccaaagagag
    16981 aatagctact atgtatttcg aatccggtga gcgccagaaa cattattaac agagcctgag
    17041 tccagtgcca aaatctctca aaacccttgt aaacatatac tttcttggta tttttcataa
    17101 tctttatgtt gtgtttattt tatctgattt tttctacttg caaatactct tatcgtacca
    17161 tgaataagaa ctccgataaa tgaaaagata atcatcagct taccaaatgt atcaatcccc
    17221 acatgataat cgcggcctgg catataaaaa tctctcaaat cattaagcct tccatctttt
    17281 cgtgtatgac attcactaca acttaccgac tcacttgcta atgataccat gtgattaaca
    17341 ggaaggaata actgtgttgg aataaagtcg taggtgccgc tgtagggcag atccagatac
    17401 tccatgccgg ttctaagcgc tgcttcccaa tcgagatcaa cccataaagc tcccttattt
    17461 acttccttat cccataactt tgcttgaatg atagtcagat atacaggatc gtatggttgc
    17521 tttccacggt gtattttagt tggatagatt ttactgtttc tatcgcgata ttctccatta
    17581 agtgtattaa tgttaagcgg tagattgtcg agattaattt tttcagttat catatgatgg
    17641 tcggcagtac cattaaacca tatatattca ggtttcacca ttctttccca atggaagtct
    17701 ccttttatag atgtataggt ctcattaccc gatgtgtcaa gcagttcata aggcagacca
    17761 tctttgcgtt gtgtagctgt tgaccagtcc catgcggttt ttgtggcatt taccttagca
    17821 taaacaggta tgtggcaggt ttgacaggct acctttatgg tatgttcatt tattttacta
    17881 tttgtatgtg gaactgacgt gtggcattgt tcacaggtta cccgttgagt atttgatgag
    17941 gagacaccat aatagcgacc ctgtatctga tgattttcgg ctgtatgaca tgtaacacat
    18001 gccatgttca ttccatccac acccatatgt acatcaatgg ttcgatcggc ggtaagtaag
    18061 gctaattcaa gatcgccatg ttttacattg ttgccaccac cgccatggaa atgacaatac
    18121 ccacaattat tacgctttgg cctgccaacg tgtgcaagta tgtttttgta gtcaggtgca
    18181 tcttgtccca taggcggata accggcagcg cctcgagctt tttcataagt gccagtattg
    18241 tcatggcaaa tcaaacaatc aatattatac tcattagtga aatcaaaagt tttatcctgc
    18301 catccatatc ctatgtggca tcggttacaa gttccttcgt tggaggaaat gccggtacaa
    18361 aaattattaa tcaaggtctt tttcccaaga tatgtaacac ctttaccagg aatataggct
    18421 tcacgctccc atttccaatg agaatttttc atcacttcct gccctcgttc ggtatgacat
    18481 gaaaggcaag ctgctgtgat caaatgtgga ttggtaaatt cttgctgtaa aacctcatgt
    18541 ttactatgat caaccccggg tttatgctcg atttcataag tctctctgag gttattaagt
    18601 aaagtgtgat ctgcaggagt ttcccgcagt attatcgtaa taattactaa cggcgctact
    18661 atagccaaca aaaaagtaat aacagtacgt aaattctgca tgattcttaa tgtattacta
    18721 aaaatctctt attaaaatag atcaaattga cgaaaaagag gtaaaataac tttagtacaa
    18781 aagtaattct ttaaatatat agattactac ttacaaatat tttcatttct ctttttttgg
    18841 tgtttcgtgt ggaaaaatat atttaaaagc aatactgaaa tagtctccag ccggtttaat
    18901 cactaaatta ctgatattaa tatcgttatg catgtataaa atctgagtat aataaagtgt
    18961 tgaaaagagt ctgaataccg gtccgatgta aaaggagccg cttttctctg tacgatattc
    19021 gaacccaaaa ccacccctga ataccggcat tacccagtag ttacgccatg atatttgtga
    19081 aaaatcataa atgtatccgc caatgtcgtt ttgcaatgat ttgcgagatt ttagctcgcc
    19141 aggcagaaac tgaaacgaaa aaccgacaga gccatccata taaacttttt cgccgaggcg
    19201 aacaaaataa ttggccgtaa gtgggatttc aaaattatca acggtatatc tcagttcaag
    19261 tgtgaagtca ttaagctctg tagctgttat tttcgaatcc cgtttaaggt aattaatccc
    19321 ggtttggagt gcaagctttg gatggaaatg ataagaaaca cccgccccat aaccaattcc
    19381 tctgagtgga tcggaagaaa actctacagc atcaacatag gcttttacgt tgtttgacct
    19441 gatcaaatcg ctcggaataa gcggttcaac ctgaaggctg aatgtaagag tcttgccttg
    19501 gccaaacaga tttacgtaaa aaagagaaag tattaaaaca agaatgtgtg aacgaaaaga
    19561 gatcattaat agttaaataa atctgattcg gtcaatttta tcactttgcc gaatcgtttt
    19621 tcaagttcat caaaattcat ttgatcacga ttgccaagaa taacgtaggt cttaggtttt
    19681 tcttttatat atttctcatt aaaggctttc atctcctcca atgacatttt tggtgtctga
    19741 ctgtaaaaca atttcctgcg gtcggtatca aatccgaatt tcttatcatt cagataagta
    19801 taaatgattc ccgacttagt aattctttcg gtttgaatat cggtaattat ctgctcttta
    19861 gccaattgga aggcgttttt cgaaagaggt atgttgttga acagatcgtt aaaagcttca
    19921 aaagcttcaa ttaccttgtc gttttgtgta gcaatgaagc ttacgttcat aaataccccc
    19981 tccggtgttg agggtgaacg ataagcagct gatgaagagt aagctaatcc acgtttttcc
    20041 ctcatctcct gaaatacaat tgcattcatt cctccaccaa aataggcatt atacaagttt
    20101 atctcgggga gtagctcttt agagtatggt atccctttgg taattgtctg aagataggac
    20161 tgattagcat cataatgagc aaaatagatt atttccttgt cagtatcaag gggttcaaag
    20221 cgtgatgctt tatccacagg taaaagcctt tttggagctt tatggtgtat ctcagttaat
    20281 ttagtaagct catcaagtga ttgtggagca taaaatgcaa tgctgtgctc caactgccat
    20341 attttttgca atgaggaggc gaggtcggca atggttaatt tatccaattc atcattagta
    20401 agtgtgaatg tagtaggatt tttctctcca taagttgcat aatttacgag agcttggaag
    20461 ttggcccgtt ggttagcttt gctgtcgagg cgagattttt taacgttttt tacatacaac
    20521 tccaagtcac ttattttagc tgtagcatta gctacaagat tttcgaaaag ccctaccgcc
    20581 tctgtaaggt tttcactaag cccgctcaat atgattcttg tttcttcgtc agtaactcta
    20641 atactaaaat tgcaggcaag cttataaaac tcgttactta tttcttccgc cgacattttt
    20701 cctgttccca taaaatcaag aaacgacgct gccaacggta acaacctatc atggttgctt
    20761 ccataaggaa aaatatagct catgctgaat gtagggtttt caacattttc gatatacaat
    20821 aattctaatc catttgtaag cttgttgcgt tt
//
LOCUS       DAOOAI010000030        20758 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_3380_length_20758_cov_5.139207, whole genome shotgun sequence.
ACCESSION   DAOOAI010000030 DAOOAI010000000
VERSION     DAOOAI010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20758)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20758)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20758
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3380_length_20758_cov_5.139207"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(197..763)
                     /locus_tag="PK345_03545"
     CDS             complement(197..763)
                     /locus_tag="PK345_03545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HPX34127.1"
                     /translation="MPTKIRLQRHGKKGRPFYHIVIADGREPRDGRFIERLGTYNPVT
                     IPAEITLDFDRTLYWLQVGAQPTNTVRSLLSYKGVLYKNHLLKGVKKGALTAEEAELK
                     HKEWLEAKQQKFIDSLKDLQNKKRQETKKALEEETKVKEKRSEEIAKKRAEEIEKQVK
                     AAQAKQQAEQEAADAEEKSNTETAESKE"
     gene            complement(975..2603)
                     /gene="pruA"
                     /locus_tag="PK345_03550"
     CDS             complement(975..2603)
                     /gene="pruA"
                     /locus_tag="PK345_03550"
                     /EC_number="1.2.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006562 - proline catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-glutamate gamma-semialdehyde dehydrogenase"
                     /protein_id="HPX34128.1"
                     /translation="MSHGFFNVPKPCNEPVLSYAPGTKERSEVKAMLSQMRGQLVDIP
                     MFIDGQEIRTNNKREIRVPHDHKHLLGHYHLGDRSHVLKAIDAALEARKKWKDLPWQH
                     RAGIFLKAADLISGKYRAKINAATMLCQSKNIFQAEIDAACEFADFLRFNVYYMTEIY
                     KEQPISAPGTWNYLEQRPLEGFIYALTPFNFTSIAGNLPSSPAIMGNVVVWKSASTQV
                     YSANVIMNIFREAGLPDGVINLVNVSGAEAGKVVSEHPDLAGFHFTGSTEVFKSMWKA
                     IGDNIMTHKTYPKIVGETGGKDFVMVHKSANPKQVATALSRGAFEFQGQKCSAASRAY
                     IPKSLWKEVREHLVADLKSFKMGGVEDFRNFINAVIDEPAFNKISGYIANAKKDGIEI
                     VAGGNYDKSKGYFIEPTVLLVNDPHYTTMEEEIFGPVLTAYVYDDNKFEETLELLDKT
                     SPYALTGSIFANDRYAVEKAYNALEGAAGNFYINDKPTGAVVGQQPFGGARASGTNDK
                     SGSKLNLLRWVSPRAIKENFDPPVDYRYPFLDAE"
     gene            2996..5938
                     /gene="dnaE"
                     /locus_tag="PK345_03555"
     CDS             2996..5938
                     /gene="dnaE"
                     /locus_tag="PK345_03555"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPX34129.1"
                     /translation="MIINAHSYYSLRYGTIPVKELVSKAALLGIKTLALTDINNTTGI
                     IDFVKACKQNGIKPIAGIEFRSNGLLRYIGLAVNNEGFRELNEFLSQHNLEEAPLPER
                     PPDFANVFIIWPLNNIPHHRLRDHEHIGIKPGEITKMMTLSSRFARSKLVILHTVTFI
                     NRDDFVLHQNLRAIDNNTLITMLTPRDLAGKDELLLSNNEIILPYIDFPEIIENTRRL
                     MDCCSIDYEYGTNKNKQIFAASRYDDKILLEKLAFDGMVYRYGKHDKKALQRVNHELE
                     IIDKLGFSTYFLIAWDIVRYSMSRGFHHVGRGSGANSVVAYCLKITDVDPIELDLYFE
                     RFINPMRTTPPDFDIDYSWDERNEVLDYIFKRYGRRYTALMGTIQRFKDRSPLRELGK
                     VYGLSKSEIDELVDFPEEHHNSNGISNQILKIAAQMNEFPNIRSIHAGGVLISEKPIT
                     YYTSLDLPPKGFPTTQWDMYVAEDIGFEKMDILSQRGIGHIKECADIIRRNRRQEVDV
                     HDIARFKKDVKLKAQLKRAETIGCFYIESPAMRGLLTKLRCDNYLTLVAASSIVRPGV
                     ARSGMMREYIYRFHNPHDFEYIHPVMEEQLKETYGVMVYQEDVLKVCHHYGGLNLAES
                     DVLRRAMSGKVRGKEEFQRIVERFFQKSKQLGRDEATTKEVWRQVESFAGYAFSKAHS
                     ASYAAESYQSLFLKAHYPLEFMVAVINNFGGYYNTWVYFNEAQRCGAKIELPCINQSD
                     YKTTIYGERVFVGFIHIQNLENKTSLKIVEERRINGVFADLEEFIQRVRPTLEQLILL
                     IRAGALRFTGISKSRLLWDAHLFFGKSKETINQTALFTSPKRNFNMPHLEHNIIEDAY
                     DEIELIGFPVSIRYFELLKTSFRGEIMAKEMATFKGKTVRMMGELVTRKFTKTKSGQI
                     MNFGTFIDNEGEFFDTTHFPKALDQWPFRGHGIYLILGKVEEEFGFYTLIVEKMAKLP
                     IVPDPRSA"
     gene            complement(6041..7243)
                     /locus_tag="PK345_03560"
     CDS             complement(6041..7243)
                     /locus_tag="PK345_03560"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HPX34130.1"
                     /translation="MSYFRVILIFLLIFLRCNTSTFTSGDINIDFPEIEIPQIPFSED
                     QLQHIEKEIVGMLDKSHFNGNVLVAYKGYPIVRYSNGYRHFFKKEKLNHETVFQLASV
                     SKAFTAMAVLMLHQQQAIDIDDPLCKYIPEFPFEDITVKNLLQHTSGLQNYMYYVDNY
                     WEKDNPLNYDDVLSLLIEHNATLSFRPGKRHYYCNTGYVMLAMLVERISGMPFYQFMK
                     LNIFNPLDMNHTFVWNQRTMDTITNIATGFKRYKRYYTSVGHDPLDEISGDKSVYSTI
                     DDMLKWERAWSNNLLISDSLRNCAFTKAETGKKRLHDYGMGWRFAEIDLKPVIFHNGL
                     WNGFTTSLHRFVEDSITVIVLNNTNGKAPVLARQLYTKALKEIEAVKNLPKAEDRLLA
                     QDSSFFMK"
     gene            7371..8045
                     /locus_tag="PK345_03565"
     CDS             7371..8045
                     /locus_tag="PK345_03565"
                     /inference="COORDINATES: protein motif:HMM:TIGR00556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HPX34131.1"
                     /translation="MVRLFAVKIIERNEFERYMPFIRDTVENNNNSPLIGHYTIKQRS
                     LLGKMMTHKILSSEFGISPDAIKIITNEHNKPALESQSNIHFNLSHSGDWVIAAFSSK
                     PVGIDIEKIKPVNLQIAWRFFSENEVEFLKSLPEDIREHWFLKLWTIKESYLKALGTG
                     LSRPMRSFSVNYRNGDFQIIDTENPTEVYLKHQNFKINYLLSVAAFKNKISDEITIYE
                     AQDLLE"
     gene            8572..10665
                     /locus_tag="PK345_03570"
     CDS             8572..10665
                     /locus_tag="PK345_03570"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008763172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPX34132.1"
                     /translation="MKKLQWFMAISIAFSVFACQQTVDQQSATEKIIEKPSITLTNDR
                     ITPEVLWAFGRVSDVKVSPDATTLLYGVTYYSIEDNKGNRELYTINVDGSGLKRITTS
                     VKSEFNAIWRPDGKKIGFISSESGTPQLWEVNPDGSGKQQITNVEDGITGFQYSPDQT
                     KILYSKELPKKNQFEDLYKDLPLATGKLNNDLMYRHWDSWVESISHIFIADYNGKEIA
                     NDKDILDGEPYDSPTKPFGGMEEVAWSPDSKTIAYTCKKKTGKDFTLSTNSDIYLYNI
                     ETGETTNITEGMMGYDMAPTFSPDGKYLAWESMERDGYESDQNRLFLIDLQTNEKIYA
                     TQSLDLDVRDYVWSNDSESIYFICDVQGTHQIYHYQLTNNSIRRITEGLHDIKTIAIA
                     GDVLIGQKQSMALPTELFAFDIQTGEERQITFTNKELLDQLTMGKTEGRWMTTHDGQQ
                     MLTWIIYPPHFDSTKKYPALLYCKGGPQGPLSQDFHYRWNFQIMAAHDYIIVAPSRRG
                     VSGFGQAWQEQISGDYHGKSMQDYLTAIDIMAKEPFVDKERLGAVGASAGGHAVFYLA
                     GNHQGRFKAFIAHDGIFNKETQYLETEEMWFVNWDMGGPFWDFNNETAQSTFKHSPHH
                     YVQNWDTPILIIHGEHDYRVVYTQGMAAFNAAVLRGVPAEFLFFPDEHHFVLKPQNAI
                     LWQRAFFNWLDKWLK"
     gene            11004..13115
                     /locus_tag="PK345_03575"
     CDS             11004..13115
                     /locus_tag="PK345_03575"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018338615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family metallopeptidase"
                     /protein_id="HPX34133.1"
                     /translation="MRYLFLSILIITGIMLQNCAPKQENPLLSEFTAPFGSPPFDKIT
                     IDHYLPAFEEAIKVHQSEIDAIVNNDEPADFDNTIVAFDKSGELLSQIGSIFSGLNGA
                     NTSPEMQELAKTTTPLLTAHRNEIRFNQQLFEKIKTVYEQRAELNLDQEQNRLVEKIY
                     DDFARNGAALPEEQRNELKALSERLSMLSLELGQNLLAENNDFKLIIDNEEDLAGLPP
                     SVIDAAADEAKNSGNEGKWVFTLAKPSWIPFLQFSERRDLREKLYRAYYMRGDNNNKT
                     DNKKPFIELMQLRQKMAEILGYKNYAEYFISDQMAKTPENVYEFLYKVWEPSIARAKS
                     ERDELQAIIDREGNGFQLESWDWWYYAELVRKEKYDLNEDELKQYLTLDNVREGIFKL
                     TNNLYGLTYQKRTDVPVYHPEVEAFEVKDNDGSHLGLLFIDPHPRPGKRGGAWCGTYR
                     SGSWKNGERVSPIVTMVMNFTRPTESQPALLSWDETTTYFHEFGHALHNLFANGKYNR
                     TSRSVPRDFVELPSQILENWASDPELLKTYAIHYQTGEPMPDELIYKLQNSKYFNQGF
                     ENAEYLAAAILDMDWHTLAVTDQTDVNEFEKETLDRIGLIKEIIPRYRTTNFNHIFGS
                     GYAAGYYVYRWAGVLDADAFAAFKESGDLYNQELAAKFRKYILAENSLWEGMEAYSKF
                     RGHEPSIEPFLERSGLLEHLN"
     gene            complement(13120..13242)
                     /locus_tag="PK345_03580"
     CDS             complement(13120..13242)
                     /locus_tag="PK345_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34134.1"
                     /translation="MEDTPDAKSFMILSMVCLVIKQQPLIRIVKRGVAARFVIN"
     gene            13359..13979
                     /gene="recR"
                     /locus_tag="PK345_03585"
     CDS             13359..13979
                     /gene="recR"
                     /locus_tag="PK345_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013265871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HPX34135.1"
                     /translation="MSAYSSKLLEQAVDELSKLPGIGRKTALRLALHLLQGSVDDVEK
                     LSDSLVKMRKDIRHCRICNNISDQEVCMICSNPRRKNSVICVVEDIRDVMAIEKTSQF
                     NGLYHVLGGIISPMEGIGPNDLTFDLLVERLSQNEISEVILALPATIEGDTTSFYIYR
                     KIKDFKVLITTIARGVAIGDELEYADEITLGRSIIKRIPYEKAVNG"
     gene            14091..14252
                     /locus_tag="PK345_03590"
     CDS             14091..14252
                     /locus_tag="PK345_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34136.1"
                     /translation="MTKPVLRQVQQPSKVTWLLGEVEAQQLGTCKTPPCNLWNFIDEP
                     DPKSNFNRE"
     gene            14412..15419
                     /locus_tag="PK345_03595"
     CDS             14412..15419
                     /locus_tag="PK345_03595"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002378.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HPX34137.1"
                     /translation="MRKKKIAVIAGGDSGEFGISMNSGKMVASILDNNLYDVYLIKIK
                     GKEWSYEKENQTFAIDKNDFSLLINENRIHFDCVFCAIHGSPGENGKMPAYFEMLNIP
                     YTSSNSLTSAITFNKHICNTIVSNLNIVNVAKSLHFFDYEKIDTDAILNYLDLPLFVK
                     PNSGGSSVGISKVKQPDSLLTAIKMAFIEDSEILVEEFVEGRELTCGVMEATGKLYVF
                     PVCEVKSKNEYFDFESKYDPLLADEIIPAPISESLDVNIKKISAYLYKKLNCAGVVRF
                     DYIYSSKRRKLFFLEVNTVPGMTGESIVPKMAMEMGIELPNLYKMMIEDALERKSQLE
                     K"
     gene            15416..16708
                     /gene="mtaB"
                     /locus_tag="PK345_03600"
     CDS             15416..16708
                     /gene="mtaB"
                     /locus_tag="PK345_03600"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HPX34138.1"
                     /translation="MKKIAFKTLGCRLNQYETDALVSQFATNGYEIVDFSKNADIVVI
                     NTCTVTDQSDQKSNQMIRQATRRHPGAMIIVTGCMVNNYRQRLKNDPDNLTWFVDNEH
                     KSDIFSLVDGHYNGEIIHPEQGNSGLFGYPPASKTLHTRSWIKIQDGCDNFCAFCIVP
                     KVRGRAVSRPYEDILDNIKQVVGFGFKEVVLTGVNIARYSYDNVDFENLIEKVLNLHE
                     DFRLRIGSIEPDGFGDKLISLFEHPKLTPHLHLCLQSGSDEILLKMRRMYAVTDFLNI
                     VDKIRKRYPLFNFTTDLIVGFPSETEDDFNKTIEVCRQIGFGHIHTFKYSVRKGTRAE
                     RMPDQIAENVKVARSEKIRDLSMELKHNYRKQWLGKTQNVLVEKNLFGTSYGYGENYL
                     PVKFKDRGRLTNYFAAVKITNIEPNGDEPALIGIKTDF"
     gene            complement(16952..18322)
                     /locus_tag="PK345_03605"
     CDS             complement(16952..18322)
                     /locus_tag="PK345_03605"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPX34139.1"
                     /translation="MKRLIFVLFVVLTVFVSCNRSSQNRGKGPVSKVAHPEWIRNANI
                     YEVNLRQFTNEGNISSFIKHLPRLRNMGVDVLWFMPIHPIGELNRKGALGSYYSIQDY
                     KLVNSEFGVIEEFQMMVKLAHELGMYVIIDWVANHTAWDHEWVTKHPDWYAKNSSGEM
                     YSPHDWTDVVQLDYSNQELRQAMLDAMAFWVREADIDGFRCDVADLVPLDFWEQAREE
                     LEEIKPLFMLAESDKEPLLINAFDMDYGWKLHHIANKIAIKEADANSIQRYFDGLENS
                     FPKGAIRMNFTSNHDENSWNGTEFERLNDGAEAFFVLMATVPGMPLIYTGQEAALKKR
                     LSFFDHDPVDWSDVPLTSFYRTLLNLKKRNSALWNGNWGAPLKRVFTNNDKAVYAFTR
                     SMDNDAVMVILNLTESEQQVQFQSEDHIAEYVNVFTNQTLVMEKDMQMTLKPWEYIVL
                     EKTEKK"
     gene            complement(18682..19578)
                     /locus_tag="PK345_03610"
     CDS             complement(18682..19578)
                     /locus_tag="PK345_03610"
                     /inference="COORDINATES: protein motif:HMM:TIGR02145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HPX34140.1"
                     /translation="MKQIRTFFIIVALFAAYSIQAQVSISDGSGRDPSAMLDVKSTSK
                     GILFPRMTTAQRDAIANPAEGLTIYNTTNRCFEGYSQGGWHLWGCLTEYGEPHDLNCD
                     NEFIDPRDSTKYNTVKIGNQCWLAENLRYLPSVSDTNTYSQTDPYYYVYGYYGTDTAA
                     AKATTNYQKYGVLYNRPAAMEACPAGWHLPGKAEWLILINYAGGKPVAGGKLKATSGW
                     GWGSSVQGTDDYGFTARPGGQFLLEGGVPKFKDELGSALWWCGEYLHGNPCAWRILHS
                     NPAIESYPTTRETGFSVRCIRD"
     gene            complement(19775..20683)
                     /locus_tag="PK345_03615"
     CDS             complement(19775..20683)
                     /locus_tag="PK345_03615"
                     /inference="COORDINATES: protein motif:HMM:NF021143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FISUMP domain-containing protein"
                     /protein_id="HPX34141.1"
                     /translation="MKPIRVIFIIIALLTTYLLEAQVSISSDGSEPGPSAMLDVKSTS
                     KGVLIPRMTTAQRDAITNPAVSLLIFNITTQCYEGYSTQDKQWYSFGCIGSYPPGARH
                     CGGTPTAIVDVTNPTTGKTWMDRNLGASRVATDSTDALAYGDLYQWGRFSDGHQCRTS
                     NTTTTLSSTDNPGHGNFIITSNSPYYDWRSPQNDDLWHGLSGINNPCPRGYRLPTEVE
                     LNIELGSWGAKSNGTGAFNSPLKLTKAGGRNYGNGSLLDVGTKGYYWSSTVSGIGSQL
                     LEFNFTSASITNHSRANGRSVRCIRD"
BASE COUNT         6461 a         4329 c         4120 g         5848 t
ORIGIN      
        1 aaagtatttt gctttgttca atggaatcgg gtaaagtaaa atcggagata acagtaatat
       61 ttgccgtttt tttatttacc tttacgatcc ggccaatttt tgtaaaatcc tctttattca
      121 taataaaaaa accggaagat cagtgttata cagatacctc cggtattcaa acatttataa
      181 aataaaaact aactcactat tccttacttt cggcagtttc cgtattcgac ttttcttcag
      241 catccgcagc ttcctgctcg gcctgttgct tcgcttgagc tgctttcact tgtttttcaa
      301 tctcttcggc acgcttttta gcaatctctt cactgcgttt ttctttaact ttcgtctcct
      361 cttctaaagc tttcttagtc tcttgccgtt ttttgttctg caaatctttc agcgaatcaa
      421 taaacttttg ttgtttagct tcaagccact ctttatgctt tagctcggcc tcttcggcgg
      481 taagagctcc ttttttcaca cctttcaaaa ggtgattttt gtacaacaca cctttgtacg
      541 ataataacga gcgaacggtg ttggtaggtt gtgctccaac ctgcagccaa tagagggttc
      601 tatcgaaatc caaagtgatc tcggctggaa tcgtaacagg gttataagta ccaagccttt
      661 cgataaatct tccatcacgt ggttcccgac catccgcaat aacaatatga taaaagggac
      721 gccccttttt accatgtctc tgcaatctga tttttgttgg catattactt taataaatga
      781 taaattgatt ttaaaattcg ggcggcaaag atcgtacttt ttcatgaata gcaaaatata
      841 ttcaatcaaa agttaaaaaa taccctttaa ccgcaaaact ttctcatgct aaaatcaaaa
      901 aaatccctcc gtgcaaaagg ttgcaaggag ggatatttca ttttcctaaa gatagactca
      961 aggaattagt tttattattc agcatccaaa aatggataac gataatctac tggtgggtca
     1021 aaattttctt ttattgcacg tggcgatacc cagcgtagca agttaagttt tgatccggat
     1081 ttgtcgtttg tgcccgaagc tcttgctcca ccaaatggct gttgtccaac gacggctcct
     1141 gtaggcttat cgttaatata gaaattgcct gccgcgcctt ccaatgcgtt ataagctttc
     1201 tcaacggcgt atctgtcgtt ggcaaagata gaaccagtga gtgcataagg agatgtttta
     1261 tccaacagct caagagtttc ttcaaatttg ttatcgtcgt aaacataggc tgtcagtaca
     1321 ggtccgaaaa tctcctcttc cattgtagta taatgtgggt cgtttacaag gagtacggta
     1381 ggctcaataa agtatccttt tgatttatca taattgccgc ctgctacaat ctctattcca
     1441 tcttttttag cattggctat ataaccagat atcttattaa atgcaggttc atcaattact
     1501 gcgttgatga aattgcgaaa atcttcgacg ccacccattt tgaaggattt gagatcggca
     1561 acaaggtgtt cccttacctc tttccaaagt gattttggaa tataggcacg cgatgcggcc
     1621 gagcatttct gaccctggaa ttcaaaagct cctcttgata gcgctgtggc aacttgtttt
     1681 ggattagctg atttatgcac catcacgaaa tcttttccac cagtttcgcc tacaatcttt
     1741 ggataggttt tatgagtcat aatattgtct ccgatggctt tccacatact cttaaaaacc
     1801 tctgttgacc ctgtaaaatg gaaaccggca agatcaggat gttctgaaac aactttaccg
     1861 gcctctgctc cgctcacgtt aaccaaattt attactccat caggcaaccc tgcttcacgg
     1921 aaaatattca taattacatt cgctgaataa acctgtgtgg aagcagattt ccaaactaca
     1981 acattaccca ttattgctgg ggatgaggga aggttaccgg ctatagaagt aaaattaaaa
     2041 ggagtaagcg cataaataaa accttcgagt ggtctttgtt ctaagtagtt ccatgttcct
     2101 ggcgccgaaa taggttgctc tttgtatatc tctgtcatgt aatatacatt aaagcgtaag
     2161 aaatcggcaa actcgcaagc tgcatcaatt tcggcctgaa aaatattttt cgactggcag
     2221 agcatcgtag ctgcattgat ctttgcgcga tactttcccg aaatcaagtc ggctgcttta
     2281 aggaaaatac cggcgcgatg ttgccatggc aaatctttcc atttcttacg tgcctctaaa
     2341 gctgcatcaa tagccttaag cacatgcgaa cgatcaccaa gatgataatg cccaagcaga
     2401 tgtttgtggt cgtgtggtac tctgatctca cgcttattgt tagtcctgat ctcttgacca
     2461 tcaataaaca ttggaatatc aaccaactgg ccgcgcatct ggcttagcat agcttttact
     2521 tcagatctct ctttcgttcc gggagcatag ctcaataccg gttcattgca aggtttgggg
     2581 acattaaaaa atccgtgtga catagtgttt aatttgttaa tatattaatt aataagcatt
     2641 gacaataaat gataattgaa tcaatgagtg caaagatagc ctttttaagc ctctgccgcc
     2701 aaccttacaa tgccccgaaa tcgggaaatc agattttttg ccaaattctc aacctacata
     2761 atacaatttt taaaagatga acaccttgaa ataaaactat aatgccatga ttaacaaagg
     2821 tttcgcaaat acataagtta cttttctttt actcaaaata aactaaacaa ctgccctatt
     2881 atctattcta acaaattctt tcaagcattt ttttagaatt tgccatgcaa cttttataaa
     2941 aatgattata ttgcaggtaa tattttgatt aattattaat cataaaaaag actgcttgat
     3001 tatcaatgct cattcatatt atagtctccg ctatggaact attcctgtaa aagaattagt
     3061 tagcaaagct gctttgcttg gtataaagac attggcactt accgacatca acaacacgac
     3121 cggtattatt gattttgtaa aagcctgcaa acaaaatggc attaagccaa ttgcgggtat
     3181 agagttcaga agtaatggac ttttacgata catcggcctt gctgtaaata atgagggttt
     3241 tagggagtta aacgagtttc tttcgcaaca caacctcgaa gaagctcctt tacctgagcg
     3301 acctcccgat ttcgccaatg tttttatcat ctggccgcta aacaatattc cccatcaccg
     3361 gctaagggat catgagcaca taggaataaa accaggggaa atcacgaaaa tgatgacttt
     3421 gagcagccgg ttcgcccgtt caaagttggt gattctccac actgttactt tcataaacag
     3481 agatgacttt gtgcttcatc aaaaccttcg tgcaattgac aacaacacac tgataaccat
     3541 gctcacgccg cgagatttag ccggcaaaga cgaactctta ctctctaata acgaaatcat
     3601 cctgccctat attgattttc ctgaaataat tgaaaatacc cgtcggctca tggactgttg
     3661 tagtattgat tatgaatacg gaaccaacaa aaacaaacaa attttcgccg ccagtcgtta
     3721 cgacgacaag atattgcttg agaagttagc gtttgatggc atggtttacc gttatggtaa
     3781 gcatgataag aaagcgcttc aacgggtaaa tcacgaactg gaaatcattg ataagcttgg
     3841 attttcaacc tacttcctca ttgcatggga cattgtgcgt tactcaatgt cgcggggatt
     3901 tcatcatgta gggcgcggca gtggcgctaa tagcgtcgta gcatattgtc ttaaaatcac
     3961 agacgttgat cccatcgagc ttgatcttta tttcgaacga ttcatcaatc ctatgcgcac
     4021 taccccaccc gacttcgata ttgactattc gtgggatgaa cgtaacgagg tgctggatta
     4081 tatcttcaaa cgttatggcc gccgttatac tgctcttatg ggaactatcc agaggttcaa
     4141 agatcgctcg ccgttgcgcg agttgggcaa ggtttatggt ctatcgaaat cggaaattga
     4201 tgagctggtt gactttcctg aagagcatca caatagtaac ggtatctcaa atcaaattct
     4261 aaaaatagcc gcacaaatga atgagtttcc caacatccgc agtatccatg ccggcggagt
     4321 gctcatttcc gaaaagccca taacctatta cacgtcgctt gatctgccac caaagggatt
     4381 ccctacaaca caatgggata tgtatgtagc cgaagatatc ggtttcgaaa agatggatat
     4441 cctgagccaa cgtggcattg gtcatataaa agagtgcgct gatataatac gtcgcaaccg
     4501 aaggcaggaa gtagatgttc acgatatcgc caggtttaaa aaggatgtaa agctaaaagc
     4561 gcaactcaaa agggccgaga caattggctg tttctatatc gaaagcccag ccatgcgcgg
     4621 gctactcacc aagcttcgct gcgataatta tcttaccttg gtagctgcca gctctatcgt
     4681 gcgcccgggc gtagcacgct caggcatgat gcgcgagtat atttaccgct ttcataatcc
     4741 gcacgatttc gaatatattc atccggtaat ggaagaacaa ttgaaagaga cttatggagt
     4801 gatggtttac caggaagatg tgctgaaggt ttgtcatcat tacggcgggc taaatctggc
     4861 cgagtcggac gtgctacgta gagcaatgag tggtaaggtt cgcggtaaag aggaatttca
     4921 acgtatagta gagcgttttt ttcaaaagtc aaaacaattg gggcgggacg aagcgacgac
     4981 aaaagaggtt tggcgtcagg tagagtcgtt tgccggatat gcattctcga aagcccattc
     5041 agcttcgtat gctgctgaga gttatcagag tctttttctc aaagcacact acccgcttga
     5101 atttatggtg gcagtgatca acaacttcgg cggatattac aacacatggg tatatttcaa
     5161 cgaagcgcag cgctgtggag caaaaataga gctgccatgt ataaaccaaa gcgattacaa
     5221 aactactatt tatggcgagc gtgtgtttgt cggttttatt catatacaga acctcgaaaa
     5281 caaaaccagc ctcaaaatcg tagaggagcg ccgtatcaat ggcgtctttg ccgaccttga
     5341 agagttcatt caacgtgtac gccctaccct ggaacaactt attttactta tacgtgcagg
     5401 agcattgcgg tttacaggta tttcaaaaag ccggttgcta tgggatgcac atctcttttt
     5461 cggcaaaagc aaagagacaa tcaatcaaac cgctctgttc acatcgccaa aaagaaattt
     5521 taacatgccg catctggaac ataatattat agaagatgct tatgatgaaa ttgaactaat
     5581 tggcttcccc gtttcaatca gatattttga gttgttaaaa acctcttttc gcggcgaaat
     5641 tatggcaaaa gagatggcaa ccttcaaagg aaaaacagtg aggatgatgg gtgagttggt
     5701 aacacgcaaa ttcacaaaga cgaaatcggg gcaaattatg aacttcggca cttttattga
     5761 caacgaaggc gaattcttcg acaccacgca cttccccaaa gcgcttgatc aatggccatt
     5821 ccgtggacat gggatatatc tcatactcgg caaagtagaa gaagagttcg ggttttacac
     5881 tcttatagta gaaaaaatgg caaaactccc aattgtaccc gacccgcgaa gcgcataaaa
     5941 agagagcgct aacttgatat caaaattttc gctttttata tcgtcaaatg tctgataaac
     6001 ttaatcttat caaataatta tctaaacaag ttagcatatc ttatttcata aaaaatgagc
     6061 tatcctgtgc aaggaggcga tcttcagcct taggtagatt ttttaccgct tctatctctt
     6121 ttagcgcttt tgtatataat tgccgggcca ataccggcgc cttgccgttt gtattattca
     6181 gaacaatcac tgtaatagaa tcttcgacaa aacgatgaag gcttgtcgta aaaccattcc
     6241 acagtccatt atgaaaaatc accggtttca ggtctatttc tgcaaatcgc catcccatgc
     6301 cgtaatcatg caaacgcttc ttaccagttt ctgcttttgt aaaagcacag ttccgcaatg
     6361 aatcgctaat aagcaaattg ttactccagg cgcgttccca tttcaacata tcatctattg
     6421 tactataaac acttttatct ccgctaatct cgtccaacgg atcatgccct accgaagtgt
     6481 aatacctttt gtaacgctta aatccggtgg caatattggt aatagtatcc atagtgcgtt
     6541 ggttccacac gaaggtgtga ttcatatcga gcggattgaa gatatttaat ttcataaact
     6601 gataaaaagg catacctgaa atacgctcca caagcattgc taacatcaca taaccggtat
     6661 tgcagtaata atgtcttttt ccagggcgga agctgagtgt cgcattatgt tcgatcaaaa
     6721 gggaaagcac atcgtcatag ttgagcgggt tgtccttttc ccaataatta tcaacataat
     6781 acatataatt ctgaagacct gaggtgtgtt gtagcagatt tttgacagta atatcttcaa
     6841 aaggaaattc aggaatatat ttacacaagg ggtcatcaat atcaatagct tgttgttggt
     6901 gaagcatcaa aacggccatt gccgtaaaag ccttactcac tgatgccaac tggaataccg
     6961 tttcgtggtt caacttctct tttttaaaaa aatgacgata accatttgag tatctgacta
     7021 tcggatagcc tttatacgca acgagcacat ttccattaaa atgcgatttg tcaagcatcc
     7081 cgacgatctc tttttcaata tgctgcaact gatcttcgga aaaaggtatc tgtggtattt
     7141 caatttccgg gaagtcaata ttgatatcac ctgaagtaaa ggtagaggtg ttgcaacgca
     7201 gaaatattaa taaaaatata agtataacac gaaagtatga catgccggaa gagacttttc
     7261 gggcgcaaaa ataaaaattt tgttgttttc aatatctcta aaaagcttta aaaatcacat
     7321 ttttaaaaat catttagctt tgcactgatt ttcagtattt tttatcggaa atggtacgtc
     7381 tatttgcagt taagattatc gaaagaaacg aatttgaaag gtatatgcct tttatcaggg
     7441 acaccgttga aaacaacaac aactcccccc tgatcgggca ttatactata aagcagcgct
     7501 cgttgctcgg taaaatgatg acgcataaaa tactatcctc agaatttggc atcagtcccg
     7561 acgccataaa aataattact aacgaacaca acaaaccagc tctcgaaagt caaagcaata
     7621 tccatttcaa cctttcccat tccggcgact gggtgatagc tgctttttca agcaagccgg
     7681 taggtataga tatcgaaaag atcaaaccgg taaatctaca gattgcatgg cgcttttttt
     7741 ctgagaatga ggtggagttc ctaaaatctt tgccggaaga cattcgcgaa cattggtttt
     7801 taaaactttg gactatcaaa gagagctatc tgaaagcgtt aggaaccgga ctttcaaggc
     7861 caatgagatc atttagtgtg aattatagaa atggagattt ccaaataata gatactgaaa
     7921 atccaacaga agtctatttg aagcatcaga attttaaaat aaattatttg ttatcggttg
     7981 ctgcttttaa aaacaaaata tctgatgaga taacaattta tgaggcgcaa gacctattag
     8041 aataacaaat cgccagtcag atcagctttt ttctgctaca acagatggat tatgttcgat
     8101 gataaaacat tcaggaataa gtaaggtttt gaattttgtg aaaaatactg atctattacc
     8161 cacttccaaa gctttaaata aagctatttt tctattactg ggattagttt tgataccgtt
     8221 ttatcaatca tttttaaaag gttagaatta cgaacctatg attctttccg acaccacttc
     8281 ataaaatatt acattgtctg tacttcttaa aaatgcaacg ggaaaccgct atattgaaaa
     8341 gagaatgatg cttatcacaa cctccgctta cattttgcat tttcagatag ataaaaaacc
     8401 atttttaggt atattcagtt aaaataaaac agcgccgcag gagtcttgtt ttagcaaact
     8461 acgcaaaact cactttctga ataggtaact ttttcgtatt gaaccattat ttactaatta
     8521 ttcgtaactt cgctccaatt tttccgaatc attcatttaa aaaagaaata catgaaaaag
     8581 ctccaatggt ttatggcaat atcaattgcc ttcagcgttt ttgcttgcca acaaacggta
     8641 gatcaacaat ccgctacgga aaagatcatc gaaaaaccgt caatcactct tactaacgac
     8701 cgtataactc cggaagtgct ttgggcgttt ggtagagtta gcgatgtaaa ggtatcgccc
     8761 gatgctacaa cacttttata tggtgttacc tactatagca tcgaagataa taaagggaac
     8821 cgcgaattat acaccattaa cgttgatggc agcggcctga agcgcattac tacttcagta
     8881 aaaagtgaat ttaacgctat ttggcggccg gatggcaaaa aaataggatt tataagctca
     8941 gaaagcggca caccgcagct ttgggaggta aaccctgacg gtagcggaaa acaacaaatc
     9001 accaatgtag aggatgggat cacagggttt cagtattcgc ccgatcaaac taaaatacta
     9061 tactcaaaag aactcccgaa aaagaatcaa ttcgaagatc tgtacaaaga ccttccgctc
     9121 gccacaggca agctcaacaa cgatttgatg tacagacatt gggatagctg ggtagagtca
     9181 ataagccata tctttattgc tgattacaat ggaaaagaga tcgccaatga taaagacatc
     9241 ctcgatggag agccttacga ctcacctaca aagccattcg gcggaatgga agaggtggca
     9301 tggagccccg acagtaaaac tattgcatat acttgcaaga aaaaaaccgg gaaagacttt
     9361 actctctcca ccaattcaga tatctatcta tataacatag aaacgggaga aactaccaac
     9421 atcaccgaag gtatgatggg atacgatatg gcccccacct tttcgccgga tggtaaatac
     9481 cttgcatggg aaagtatgga acgcgatggc tacgaatccg atcaaaacag actatttctc
     9541 attgacctgc aaacaaacga aaaaatttac gccacccaat cactcgacct tgatgtcagg
     9601 gactacgtct ggagcaacga tagtgaatct atctatttta tctgcgacgt tcagggaaca
     9661 catcagatat atcattatca gcttaccaat aattcaatac gtcgaattac agaaggattg
     9721 catgacatca aaactattgc cattgccggg gatgtgctca taggacaaaa acagagtatg
     9781 gctctgccaa ccgagctttt tgccttcgac atacaaaccg gtgaggagcg tcagatcaca
     9841 tttacaaata aggaattgct ggatcaactg acaatgggca agaccgaagg acgatggatg
     9901 accacccacg atggtcagca gatgttaacg tggattattt accctcctca ttttgattct
     9961 acaaaaaaat atccggctct attatattgt aaaggagggc cacaagggcc gttaagtcag
    10021 gattttcact atcgctggaa ctttcagata atggctgccc acgactatat catcgttgcg
    10081 ccgagcagaa gaggagtatc cgggtttggg caggcatggc aagagcaaat cagcggagac
    10141 tatcacggaa agtcaatgca agactacctt acagctattg atataatggc aaaagaacct
    10201 tttgtagata aggagagatt aggagctgta ggagcaagcg ccggcggaca tgcagtattt
    10261 tatttagccg gaaatcacca agggcgattc aaagcattca tcgctcacga cgggatattc
    10321 aacaaagaga cgcaatattt agagacggaa gagatgtggt ttgtcaactg ggatatgggc
    10381 ggcccattct gggattttaa caatgaaacg gcacaaagca catttaaaca ttcgccgcat
    10441 cattacgtac aaaactggga tactccaata ctcattattc acggcgaaca cgactaccgt
    10501 gttgtatata cacagggaat ggcagcattt aatgcagcag tactgcgcgg cgtgccagcc
    10561 gaattcctgt tcttcccgga tgaacatcac tttgtgctaa aaccacaaaa tgccatactg
    10621 tggcaaagag cattttttaa ttggttagat aaatggctta aataaaaaca ttcactgcca
    10681 aataacgaaa aacgttttaa gcgcaagttt ataatttgaa agtttcatta agattaatgg
    10741 aattgttagc cattgttttt tttccattat ctaacaatgt ttaacatcta atttcgtaat
    10801 ttgaaaaatg gatatggcga aatggttcaa atacaaaact caataataaa aggatagtta
    10861 taaactaaca atttcgacat tctgctcaaa tgccagaata tcctatttgg ctaagttttc
    10921 ttaaaagctt tcattacttt tgcaatgttt ttgaaattat cttaacgcta tcatttatat
    10981 aaatctttct taaatattta attatgaggt acttattttt atcaatttta attataacag
    11041 gtattatgct gcagaactgc gcaccgaaac aagagaatcc actgttgagc gaattcactg
    11101 ctccttttgg ctctccaccc ttcgacaaaa tcaccattga ccattatctt cctgcatttg
    11161 aggaggcaat aaaagtgcat cagtcagaga ttgatgccat agtgaacaac gatgagccag
    11221 cagatttcga caatacgatc gttgcttttg acaagtcggg tgagctactt agccaaattg
    11281 gatctatttt tagcggactt aacggcgcaa acacttcacc cgagatgcaa gagcttgcaa
    11341 aaacaacaac acccttactc actgcccatc gaaatgaaat cagattcaat cagcaacttt
    11401 ttgaaaaaat aaaaactgtt tatgagcaac gtgccgagct caatcttgat caggaacaaa
    11461 acagattagt tgaaaaaatt tatgacgatt ttgccagaaa tggggctgct ctacccgaag
    11521 agcaacgcaa tgagctgaaa gctttaagcg aacgtttatc aatgctatcg cttgagttag
    11581 gtcaaaatct tttagccgaa aacaatgatt tcaaattaat aattgacaat gaggaagacc
    11641 ttgcaggcct gccgccctca gtaattgatg ctgccgccga tgaagcgaaa aactccggca
    11701 acgaaggaaa atgggtgttt actcttgcca aaccaagctg gatacccttc ttgcagttct
    11761 ccgaacgccg cgacctgcgc gaaaagctct atcgtgcata ctatatgcgt ggggataaca
    11821 acaataaaac agataacaaa aagccattca tcgagttgat gcagcttcgt caaaaaatgg
    11881 ccgagatatt aggttacaaa aattatgcgg agtattttat cagcgatcag atggctaaaa
    11941 ccccggaaaa cgtatatgag ttcctttaca aagtatggga gccttccatt gccagggcaa
    12001 aaagtgagcg ggacgaatta caggctataa ttgatcgtga agggaacggc ttccagttag
    12061 agtcgtggga ctggtggtat tatgccgaac tggttcgtaa agaaaaatat gacctaaacg
    12121 aagatgaact caagcaatat cttaccctcg acaacgtacg cgaagggatc ttcaagctta
    12181 ccaataacct ctacgggctt acatatcaaa agcgcacgga tgttccggtt tatcaccctg
    12241 aagttgaggc ttttgaagta aaagacaatg acggcagtca tcttgggtta ctcttcattg
    12301 acccgcaccc cagacctgga aagagaggtg gagcttggtg cggcacatat cgcagcggct
    12361 catggaaaaa tggcgagagg gtatctccaa tagttactat ggtgatgaac tttacacggc
    12421 ctaccgaaag ccaacccgct ctgttaagct gggatgagac aactacatac ttccacgaat
    12481 tcgggcatgc actacacaac ctctttgcaa acggcaaata caaccgtacc agcaggagcg
    12541 tgccgcgcga ttttgtcgaa ctgccctcac aaattttaga aaactgggct agcgatccgg
    12601 aattattaaa aacttatgcc atccattatc aaaccggcga accaatgccc gatgaattga
    12661 tttacaagtt acaaaacagc aaatatttta accaaggttt cgaaaatgcc gaatacctgg
    12721 ctgccgcaat tctcgacatg gactggcaca cattagcagt aacagaccaa accgacgtca
    12781 atgagtttga aaaagagaca ttggatcgaa taggactgat aaaagagatc attccacgct
    12841 accgcacaac caactttaac catattttcg gttcgggata tgctgccggt tattacgtat
    12901 atcgttgggc tggtgtttta gatgccgacg cctttgcagc tttcaaagaa tcgggcgatc
    12961 tctataatca agagctggca gctaaattca gaaaatatat tttagccgaa aacagcctat
    13021 gggaaggcat ggaggcatat tcgaagttcc gcggtcatga accttcaatc gagcctttct
    13081 tagaaagaag cggactttta gaacatctga attaataatt taattaataa caaaacgagc
    13141 ggcaacaccc ctcttcacta ttctgatcag aggttgctgc tttatcacaa ggcataccat
    13201 tgataaaatc ataaacgatt tagcgtcagg ggtatcttcc ataaaatcag tttagcacat
    13261 aaaggctttg tgttagttaa cacgaaatca tctctattta aaaacccttg ttctcttcat
    13321 aaaaaactac ttttgcaact aaatataaaa ctattgattt gagcgcttat tcgtcgaaac
    13381 tattagaaca ggctgtggat gaactctcga aattgccggg gataggccgt aaaacagcat
    13441 tgcggttagc cttacatttg ctgcaaggaa gtgtggatga tgttgaaaag ctaagcgatt
    13501 cgcttgttaa aatgagaaaa gatattcggc attgcaggat atgcaacaac atctccgatc
    13561 aagaggtttg tatgatatgc agcaaccctc ggcgaaaaaa ctcagtaata tgtgtagtgg
    13621 aggatataag agatgttatg gccattgaaa agacatccca gttcaacggg ctttaccatg
    13681 tgttaggggg gatcatctcg ccaatggaag gcataggccc taacgatctg acttttgacc
    13741 tattagtgga gcgactttca caaaacgaaa tcagcgaggt gattttagcg ctcccagcta
    13801 ctatcgaagg cgatacaaca agcttttata tctatcggaa aattaaagat ttcaaagtgc
    13861 tgataaccac cattgcccgt ggtgtggcta ttggagatga attggaatac gccgatgaaa
    13921 taacccttgg cagatcaatt ataaaacgaa taccatacga aaaggctgta aatggataac
    13981 cgaatgggac tgtatcagaa caaatattta actaaaaaac aacaaggcat cttagaagta
    14041 ttcaaataca aggcgctgag gttgagggca aaggagcgta cttgaagtac atgactaagc
    14101 ccgtacttcg acaagttcag caaccatcga aagtaacatg gttgctgggc gaagtcgaag
    14161 cacagcagtt gggtacttgt aagacaccgc cttgtaatct ttggaatttt attgatgaac
    14221 cagacccgaa aagtaatttt aaccgcgaat agttcaactt tgtttagcaa acatgcacga
    14281 ttattaaaac atacgtcatt tacattctgc aatatcctcg acatatcaaa tatacggata
    14341 tctgaaaaaa atcgttttca aagctgactc attgatcaat taaatactgt ttcgaaatat
    14401 tagtaaaaca aatgagaaaa aaaaagatag cagttattgc cggtggcgac tcaggcgagt
    14461 tcggaatctc gatgaatagt ggaaaaatgg tggcatccat tttagataat aatctttacg
    14521 atgtttatct gatcaagatc aaaggaaaag agtggagtta cgaaaaagaa aaccagacgt
    14581 tcgccattga caaaaacgat ttcagtttac ttattaacga aaatcgtatt cacttcgatt
    14641 gtgttttttg tgccatacac ggttcgcccg gcgaaaatgg caagatgcct gcctattttg
    14701 aaatgctcaa cataccatac acttcgagca acagcttaac ttcagccatc acttttaaca
    14761 aacacatttg caacaccatc gttagcaacc tcaacatcgt taatgttgcc aaatccctgc
    14821 attttttcga ctatgaaaag atagataccg atgccatact gaactatctt gatctccctc
    14881 tctttgtaaa acccaattcg ggcggctcaa gtgtagggat tagcaaagta aaacaacccg
    14941 atagcctttt aactgctata aaaatggcat ttatcgagga ttcggaaata cttgttgagg
    15001 aatttgtaga gggacgcgaa cttacatgcg gcgtaatgga agcaacaggc aagctatatg
    15061 tgtttccggt atgtgaggta aaaagcaaaa atgagtattt cgatttcgaa tcaaaatatg
    15121 atcctttatt agctgacgag attattccgg ctcctatatc tgaatcgtta gacgtaaata
    15181 tcaaaaaaat atcggcatac ctttacaaga agttaaattg tgccggagta gtaaggttcg
    15241 attatatcta ctcatcaaaa agaagaaaac tctttttcct tgaagtaaat accgtacctg
    15301 gaatgaccgg cgaaagcatc gttccaaaaa tggcaatgga gatgggtatt gagttgccta
    15361 acctctacaa aatgatgatc gaagatgctt tagagcgaaa aagccaattg gaaaaatgaa
    15421 aaagattgcg tttaaaactt taggctgccg gttaaatcaa tatgagactg acgcattagt
    15481 ttcgcaattc gcaactaacg gctacgagat tgttgacttt tctaaaaatg ccgatatagt
    15541 agttattaac acttgtactg taaccgatca gagcgaccaa aagtcgaacc agatgatccg
    15601 acaggctaca cgccgccatc cgggggcaat gataattgta accggctgta tggtgaataa
    15661 ttatcggcaa aggttaaaaa acgaccccga caatctaaca tggtttgttg ataatgagca
    15721 taaaagcgac atcttctcct tggttgacgg acattacaac ggagagatca tacaccctga
    15781 gcaaggaaat tcagggcttt tcggctatcc gcccgccagc aaaacgctac atacccgaag
    15841 ttggataaaa atacaggatg gctgcgacaa cttctgcgcc ttctgcatcg tccccaaagt
    15901 gaggggaaga gctgtaagca ggccttatga ggatatactc gataacataa aacaagtagt
    15961 tggttttggg tttaaggagg ttgtcctcac tggagttaat attgcaagat actcatacga
    16021 caatgtggat tttgaaaacc tgatagaaaa ggtactaaat ctgcatgagg actttaggct
    16081 aaggataggc tcaattgagc cggacggctt tggcgacaaa ctgatcagcc tctttgagca
    16141 tccaaagctt accccacatt tgcatctttg cctccaaagc ggctccgatg agatactgtt
    16201 gaaaatgcgc aggatgtacg ccgttaccga cttcttgaat attgtggata aaataagaaa
    16261 acgatacccc ttgtttaact tcactaccga tcttattgta ggatttcctt cagaaacgga
    16321 ggatgatttt aacaaaacga ttgaggtgtg ccgccagata ggctttggcc atattcatac
    16381 ttttaaatat tcagtgcgta aagggacgcg tgctgaaaga atgcccgatc agatagcaga
    16441 aaatgtaaaa gtggcgagaa gcgaaaaaat cagggattta tcgatggagt tgaagcataa
    16501 ctaccgaaaa caatggttag gtaaaacaca aaatgtattg gtagaaaaaa atctttttgg
    16561 aacttcatac ggttatgggg aaaactattt acctgtgaag tttaaggatc gtggacggct
    16621 aaccaactac tttgctgctg tgaaaattac caacattgaa cctaacggcg atgagccggc
    16681 tctgattggc ataaaaaccg atttctgata ctataatctt ttgtctttgt acctctctac
    16741 taccctactg caaactccgt aaaactttgt aaaaaaaaaa agttgctcag agttgcacag
    16801 agtacacaca gagcaacaca gaggaaataa atactctgtg aaactacttg aaaaactccg
    16861 taaaactctg tgtaactaaa aaagtgtctc agaagaacgc cgagaaaaaa aactctgtgt
    16921 aactaaaaaa gcagcaagag atactacctg atcatttctt ctccgttttt tctaacacta
    16981 tgtattccca aggcttcagt gtcatttgca tatctttctc cattactaac gtctgattgg
    17041 taaatacatt tacatattct gcgatatgat cttcgctttg aaactgaact tgctgttccg
    17101 attcggtaag attaaggatt accattacag catcattatc catcgaacgt gtaaaggcat
    17161 atacagcttt gtcgttgtta gtaaatactc tttttagtgg cgctccccag tttccgttcc
    17221 ataaggcgct atttcttttt ttcaaattca agagcgttcg ataaaaggag gtaagaggca
    17281 catcagacca atcaaccgga tcatggtcaa agaagcttaa tctctttttc aaagcggctt
    17341 cctgaccggt atatataaga ggcatacccg gaacggtagc catcagtaca aaaaaggctt
    17401 cggcgccatc attaagcctt tcaaattcag taccgttcca tgagttctca tcatgattag
    17461 aggtaaaatt catgcgtata gcgccctttg gaaagctatt ctccaacccg tcaaaatagc
    17521 gctggataga attggcgtca gcctccttta tagctatttt atttgcaata tggtgtagtt
    17581 tccagccata atccatatca aaagcattga tcagcaaagg ctctttatcg gattcggcca
    17641 gcatgaaaag aggttttatt tcttccaact cctcccgtgc ctgttcccag aagtccaaag
    17701 gcaccaaatc ggctacgtcg caacgaaatc cgtcaatatc cgcttctctt acccaaaatg
    17761 ccatagcatc aagcatagcc tgccgcaatt cctgattaga ataatcgagc tgaacgacat
    17821 ctgtccaatc gtgtggcgaa tacatctcgc cactactatt ttttgcatac caatcgggat
    17881 gtttggttac ccactcatgg tcccatgcag tatgattggc tacccaatca ataatcacat
    17941 acattcccaa ttcgtgggca agctttacca tcatttgaaa ctcttcaatt actccaaatt
    18001 cggaattaac caatttgtaa tcctgtatcg agtaatagct acccagagcg cctttgcggt
    18061 ttagttcgcc gatagggtga attggcataa accacaaaac atcaacaccc atattacgca
    18121 agcgaggcaa atgcttaata aatgagctta tattgccttc gttagtaaat tgccggagat
    18181 tgacctcata aatattggcg ttgcggatcc attcgggatg cgctacttta gataccgggc
    18241 ctttacctct gttttgggat gaacggttac aacttacaaa aactgtgaga acaacaaaga
    18301 gaacaaaaat caatcttttc atagttattt accaattatc aaggtttaaa aaatcatctt
    18361 ataaattaac tttacaaaaa cagataaagt aacggcaaaa atataagtaa ttgccaattt
    18421 gataaagaca attaaacccg gatttcaaaa agagctgatt tttgagtagt tgggtaatat
    18481 ttaccgtatt cgtaacagcc cgattggctt ataaaagtaa gaactgctat taaatgcttc
    18541 aaaagttttt gatagcagaa aagaagaaac agatcaggtt aataatctta ataagagaaa
    18601 ggatgacctc ttatccaaca gataggccaa ctaccttacc ccccttaggt tagctcagag
    18661 catcgctttt acctattacc tctaatccct aatacagcgg actgaaaaac cagtttccct
    18721 tgttgtggga tatgattcta ttgcaggatt agagtgtaat atacgccacg cgcacggatt
    18781 tccatgtaga tactctccac accaccaaag agccgaacca agttcatctt tgaattttgg
    18841 aactcctccc tcaagtaaaa attgaccgcc tggacgcgct gtaaagccat aatcgtcagt
    18901 cccctgtacc gagctccccc aaccccatcc acttgtagct tttagtttcc ctcctgcaac
    18961 cggtttgcct cctgcataat ttataagtat caaccattca gctttaccag gtagatgcca
    19021 gccagcagga catgcttcca ttgcggcagg cctgttgtat aatacgccat acttttgata
    19081 atttgtagtg gctttggcag ccgccgtatc agttccataa taaccataaa cgtagtaata
    19141 cggatcagtt tgagaatatg tattagtgtc cgaaacactt ggtaaatatc ttaggttttc
    19201 ggccaaccag cactggtttc caattttaac cgtgttatat ttagtagagt ctcgcggatc
    19261 aatgaattcg ttatcgcagt ttaaatcatg gggttctcca tattccgtta aacatcccca
    19321 taggtgccat ccaccctggc tatacccttc aaagcatcta ttagtagtat tgtaaattgt
    19381 caaaccttcg gccggattgg ctattgcatc ccgctgggcg gtagtcatac gggggaataa
    19441 aatgccttta cttgtgcttt ttacatccaa catagccgat ggatcgcgtc cactgccgtc
    19501 gctgatagat acttgcgctt ggattgaata ggctgcaaat agcgctacaa ttataaaaaa
    19561 tgttctgatt tgtttcatct gtagaaaagt ttaattgttt atttaatttt gttattgtat
    19621 aaataataat cttttttgac ttagtagact gcacttaggc taagcaacac cgcaatttca
    19681 ctaactctcg gagcgcccgc tcacgggagt atttccgctt cccgctccat taccgcaatt
    19741 aggcggcggt tagccagcgc cctgtaaaaa aatatcaatc cctaatacaa cgcacagagc
    19801 gaccattcgc ccgagagtgg tttgttatac tggcgctcgt gaaattaaac tccaggagct
    19861 gcgagccgat accacttacg gtacttgacc aatagtaacc tttggttccc acatcaagga
    19921 ggctgccatt gccgtagttg cggccgcctg ccttggttag tttaagcggt gagttaaaag
    19981 cgccggttcc gttactttta gcgccccagc taccaagttc gatattcaat tccacttctg
    20041 ttggtaagcg gtaacctctt gggcaggggt tgtttatacc gcttaagccg tgccataggt
    20101 catcgttttg cgggctacgc cagtcataat aagggctatt agacgtaata ataaagttgc
    20161 catgcccggg attatcagtt gatgacaatg tagtggttgt gttagaagtt ctacactgat
    20221 gtccatccga gaaacgcccc cattggtaca aatcgccata ggcaagtgca tctgtactat
    20281 ctgtagccac ccgagaagct ccaaggtttc tgtccatcca ggtttttcct gtggtggggt
    20341 tggttacatc tactattgct gtaggcgttc caccacaatg gcgagcgccg ggaggatacg
    20401 atccgataca cccaaaagaa taccattgct tatcttgcgt gctatatcct tcgtagcatt
    20461 gcgtggttat attaaaaatc aacagcgaca ccgcaggatt ggttattgca tcccgctggg
    20521 cggtagtcat tcgggggatt aaaacgcctt tgcttgtgct ttttacatcc aacatagcag
    20581 agggaccggg ttcgctgcca tcgctgctga tagatacctg tgcctctaac aaataggttg
    20641 taagtagagc aatgattata aaaattactc taattggttt catatcaaaa aattaaattt
    20701 tttgtattaa tctttgtttt tgtataactt gtaaactttt ttgccgccgt aggctgcg
//
LOCUS       DAOOAI010000031        20746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_3384_length_20746_cov_4.917597, whole genome shotgun sequence.
ACCESSION   DAOOAI010000031 DAOOAI010000000
VERSION     DAOOAI010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20746
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3384_length_20746_cov_4.917597"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            172..1161
                     /locus_tag="PK345_03620"
     CDS             172..1161
                     /locus_tag="PK345_03620"
                     /inference="COORDINATES: protein motif:HMM:NF013383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34142.1"
                     /translation="MTKNFNDKKIFFVGSGAIATALGNVLVKTAEHDVTLITIEKEVA
                     DSINRDHYNNKYFPAIRLEKGLKASVDFSLLKQEAIVFIAIPSAVVVNFISNRDIHPD
                     SILVNLAKGFGHQHYTIVESLNKLLPNKICSLKGPSFAREIMNNQPTAMTLGAEDGTL
                     YPIFLELFKETLIYTDYTDDVYGVEILSILKNIYAIAIGVVDAQFESPNLKYLIFTKA
                     LYEMRNLLILFGGKKKTLFNYCGVGDFALTALNDLSRNRTLGLLIGKGFFTDNISEKV
                     VLEGRIAVNILYNRLLEMGLDTQEFPIIYELYKVFNGTYDISSFVINLLKNNR"
     gene            complement(1175..2467)
                     /locus_tag="PK345_03625"
     CDS             complement(1175..2467)
                     /locus_tag="PK345_03625"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPX34143.1"
                     /translation="MKKALIITYYWPPSGGAGVQRWLKFAKYLRDYGWEPVIYTPENP
                     EYPAVDHSLEKDIADNITVLKTKIKEPYNLYKRFVGRKKSDQIKSAFLSEKKTPALAE
                     KISVWIRGNFFIPDARVFWIKPSVKYLIEYLSLYSVDAIISTGPPHSMHMIALKVHQK
                     TGLPWVADFRDPWTHIDYYKDLKLTAFADRKHHKLEKQVLSKANRVVVVSQGMKSAFE
                     TIHKRHYDVITNGFDDEDIPKEIQIKSNRFTIAHIGSLTKSRNPETLWLALNKITNQN
                     KQFAQELEIKIIGKADWVAREAIRNAGIEKYVEYIDYLPHDMVVQEQVAAGVLLLIIN
                     RTENAKMIVTGKLFEYLAARRPILCIGPADGDASAIIRQTDSGLIADYDDQTLIESHL
                     ERLYNAFLTEKQLFQYRNIESYTRKNLTKELAVILDSI"
     gene            complement(2482..4920)
                     /locus_tag="PK345_03630"
     CDS             complement(2482..4920)
                     /locus_tag="PK345_03630"
                     /inference="COORDINATES: protein motif:HMM:NF021126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HPX34144.1"
                     /translation="MDKFSIKHIWPYAAAIVFFLIIMLVYFSPVLDGKLPRQDDIQRW
                     RGMSKEIVDYREATGEEALWTNSMFGGMPAYQISVIYNKVLVKHIDNILQLGIPYPIS
                     LVFLYFLGFFILLLSLKINIWHSLLGAIAFAFSSYFFIIIEAGHNSKAHAIGYMAPVL
                     AGILLAYRGKYIWGGAMTALFLSLQLMANHLQITYYLLLIVIIFGIVQLIESIKTQTF
                     PQFFKATVVLLAAALFAFSVNISNLWATWEYGKYTIRGPSELSHTPENRTSGLDIDYA
                     TQWSYGRAETMTLLIPDFMGGGSMRNPGEDSNTYKEFVKLGLPANQAKSYVKMIPMYW
                     GDQPFTSGPVYAGAIIVFLFALGAIVVKGKMKWWLIAATILSILLSWGHNLMPLTEFF
                     LRYVPGYNKFRAVSMTLVIANLTIPLLAILALKEIFDKNTDPKKMFSHVITAFYVIGG
                     ILLFFIVFGGSLFNFSSEVDNQLIQAGFPQSVIDAIRADRKAMLTSEATRSLVFIILA
                     IVAIWMGLFKKVRNEFVFAALIVLVTIDMYAVNKRYLNEDDFVDEKKVSIPYTPSEAD
                     KMILEDKDPNFRVFNLTVSPFNDVSTSYFHKSIGGYHGAKLRRYQELIDHHISKNNVD
                     VLNMLNTKYFIIEDQNKKAQANINPRALGNAWLIQNIKWAENADEEIEALNDFDPKTT
                     AIIDRRFEKLVSGFQNKDTLIGSIKLMSYKPNELIYNYQSTEDNLVVFSEIYYDKGWN
                     VYVDGKKMPYLRANYVLRAMMVSSGNHEIVWKFEPKVFFVGGKISLITSLIVILFFFA
                     ALGNELKDIFVPKK"
     gene            complement(4941..5183)
                     /locus_tag="PK345_03635"
     CDS             complement(4941..5183)
                     /locus_tag="PK345_03635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34145.1"
                     /translation="MRFLGILFLFVLAFQLISRYLFPFLLKTFIKKAAKHQGSAHHSA
                     RPEGEVTVNYNPTDTNSTKFNPKTVEDVEYEEIKEK"
     gene            complement(5362..6366)
                     /locus_tag="PK345_03640"
     CDS             complement(5362..6366)
                     /locus_tag="PK345_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34146.1"
                     /translation="MKQIANLNRDQCLKIAEVIGNLRPNPTFYEREYLTIKLKDEVKL
                     RMNFFAVAICHQTYKLHHPLLDLWGWDFIEHVFIKMAQQNSSLLNPEKVSLLSTQQIA
                     KELQRWFSHNENSNDCTLDRIEERSQLIKDASESLIAICGGQLTGLITDSKAYLIRDG
                     KGLYEVLQKIEAYSDPQRKKSTFLIKLLIDSGLLEIGDPENFIPIMDYHMQRVLMRMG
                     CLEISDPALYQILIARHPVTTDEPIRSLCIEAAKLIAIHSGHPLIRLNDFLWSLGRSC
                     CNNTTLCKDHLCEKSPCTFNQIISLKSHQKCEFETACKGFEEDKYRKLWQPVINTHYY
                     "
     gene            complement(6424..7167)
                     /locus_tag="PK345_03645"
     CDS             complement(6424..7167)
                     /locus_tag="PK345_03645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34147.1"
                     /translation="MSNRTVKFLFLFIIIQLIGCTKSTIERAPEIKAGYHSGMIINFY
                     DTTLIGGYYSQKAYNIDLDNNGLDDFQFVSWIWGSPGMGQIPQASINCLHCSAKVLGI
                     VTTDTMYLNRDTLIFEGAQPRTWDMYLMFNYSCIRISSNDTILNTNLTFKINPLERDD
                     KIRKSDPAICDSLTLTSGNKNSWPMLIGVSGDTTIYRYDIDHNNCNNFPLEKNVYLGV
                     LLDDERLGWIKINIINNFKIIIHESGIQE"
     gene            7582..7947
                     /locus_tag="PK345_03650"
     CDS             7582..7947
                     /locus_tag="PK345_03650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34148.1"
                     /translation="MKVVSRVEDVINPSIVERGSIICTAGADVTSQVLLRSLAADPVN
                     FVTENGAVNPQGLNIAEHAVSISHLAASEHRKKLLKYFYESKEYHEPPEAFGKLSLRA
                     LHFMRILLLPFFPIFSENY"
     gene            7967..9661
                     /locus_tag="PK345_03655"
     CDS             7967..9661
                     /locus_tag="PK345_03655"
                     /inference="COORDINATES: protein motif:HMM:NF015530.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HPX34149.1"
                     /translation="MKSLKLSLWLTLLIMISFSCACPDRPDNGEDQRAGIIPQDVEFE
                     ALPDLDMEVKLAEFAEFELTSNLDHLTVDERAILPILFEAASIMENIFWKQAIGNKED
                     FLNRIKDEKARMFAQIHYGPWDRMNNNEPFLTNIGLKPEGANFYPDDMTKEEFENFTD
                     PDKTSQYTLIRRDEDGNLKVLWYHEAYRVQIEKAVDLLRKAAEISENDGFKNYLNLRA
                     EALLTDDYKASDMAWMDMKNSNIDFIVGPIENYEDQMFGCKAAFESFILIKDPDWSSR
                     LQRFAALLPSLQTMIPTNEKYLKDAPGSDSDLNVYDAIFYAGDCNAGSKTIAINLPND
                     PEVHVAKGTRKLQLKNSMKAKFDKILIPIAKILIDPVQQKHVTFDAFFANTMFHEVAH
                     GMGVKNTVTGKGSVREALKETYTSIEEGKADIMGLWLVSRLREMGEISDGELMDNLVT
                     FSAGLFRSSRFGTASAHGKANMMRFNFFEEHGAFTRSDKGFYTVNMEEMLEAMNILVD
                     KIMTIQGEGDYEAAKAWIEADGNVSESLEYDLNRLNDYGIPVDIYFKQGPDVAGIK"
     gene            complement(9738..11000)
                     /locus_tag="PK345_03660"
     CDS             complement(9738..11000)
                     /locus_tag="PK345_03660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthetase B"
                     /protein_id="HPX34150.1"
                     /translation="MRKLIFTITFLFSFLLSHAAYLLIPMDNSQKNHLKAYGIAYWTL
                     EREVEVSWLLNYRGGSFMCRYYEQVERELKVRGVSFEIIADIQAAQIIREISDPEENM
                     DEIKLHKAPKIAVYSPKHKLPWDDAVTLALTYAEIPYDVVYDLEVMEGVLPMYDWLHL
                     HHEDFTGQYGKFWRSFRNAAWYQEDVRVNEEMARLLGYSKVSQMKLAVAKKIRDFVAG
                     GGYMFAMCSAPDSFDIALAADGVDIVESMFDGDPMDPNAQSKLNFDNTLAFTNFTLVT
                     NPNIYEISDIDVTETRPRTINEKNDFFTLFDFSAKWDPVPTMLCQNHMQVIKGFMGQT
                     TAFNMNTIKPNVLIMGEYKAANEARYIHGEYGHGTFTFYGGHDPEDYRHLVGNPPTDL
                     NLHPNSPGYRLILNNVLFPAAKQKKRKT"
     gene            complement(11330..12010)
                     /locus_tag="PK345_03665"
     CDS             complement(11330..12010)
                     /locus_tag="PK345_03665"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein signal peptidase"
                     /protein_id="HPX34151.1"
                     /translation="MWKKSLLIITLILIADQVLKFGIKTNMTLGEEINILGNWFIIHF
                     TENEGMAFGLSFGGIFGKLALSLFRIAAITIIFFYLREIIRKKYHKALIVSISLILAG
                     AIGNMLDSAFYGLIFSNSSYTTVAKLFPPEGGYGSFLHGKVVDMLYFPIIQSEYPAWV
                     PIVGGDDFIFFRPVFNIADSSITIGVFILIIFQGKIFRKNKPTESETLQIEEDENLNT
                     ESDSSNPI"
     gene            complement(12114..12536)
                     /locus_tag="PK345_03670"
     CDS             complement(12114..12536)
                     /locus_tag="PK345_03670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA C4-type zinc finger protein"
                     /protein_id="HPX34152.1"
                     /translation="MANQSMDSNAPEVVKTRYNKEELEEFRQIILEKLEKARADLALL
                     TEAYSNVNENDTNDTSPTFKVLEEGYNVLSKEENSRLAVRQQKFITNLENALIRIENG
                     TYGICRVTGKLIAKERLKSVPHATLSMEAKLKQYQTRK"
     gene            complement(12585..16019)
                     /gene="ileS"
                     /locus_tag="PK345_03675"
     CDS             complement(12585..16019)
                     /gene="ileS"
                     /locus_tag="PK345_03675"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011966017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HPX34153.1"
                     /translation="MVSKYKEYDNLNLIRIDQEILAYWKEHDIFHKSLKNREGSESFV
                     FYEGPPSANGIPGIHHMISRTIKDIFCRYKTLKGYYVARKAGWDTHGLPIEIAVEKTL
                     GITKEDIGKSISVEEYNRACRTEVMKYKDLWDELTIKMGYWVDLNDPYITFENKYIES
                     VWYLLSKLYEKGLLYKGYTIQPYSPAAGSGLSAHELNQPGCYRDVKDTSVTALFSVIR
                     NEKSKFLFSNKTDQVSILAWTTTPWTLPSNTALAVGSKIRYLKVQSFNQYTGKEITVI
                     LAKDLLNSYFNEKAADLPLEDYKIGDKLIPYRILDEFDGALLEGVRYEQLMPWIKPDG
                     DAFRVILGDFVTTEDGTGVVHIAPTFGADDDRVARSSDIAPLILIDKNGSKRPMVDLK
                     GRFFRIEDLDNEFVKKFVDVSLYSEYAGRYVKNDYDLEATPETPILDVDLSVMLKLDN
                     KAFKIEKYVHNYPHCWRTDKPVLYYPLDSWFIKTTAFKERMIELNNTINWKPKSTGVG
                     RFGNWLENLVDWNLSRSRFWGVGLPIWITEDRKEQKCIGSVAQLKEEIERAVEAGFMT
                     NNPLAEYISDDNSEENYSKFDLHRPYIDEIILVSDKGEKMYRELDLIDVWFDSGAMPY
                     AQFHYPFEKGDHLADYFPADFIAEGVDQTRGWFFTLHAIATMVFDSVAFKTCVSNGLV
                     LDKNGNKMSKRLGNAVDPFETIKKFGADATRWYMITNAQPWDNLKFDTDGIIEVQRKF
                     FGTLYNTYAFFALYANIDNFRYNQQEVPLEERTELDRWILSELNTLITIVDEAYDDYE
                     PTKAGRAIAHFVDYKLSNWYVRLSRRRFWKGEMSTDKITAYQTLYKCLESIAVMISPI
                     APFFSEKLFLDLNSVTKRYDVDSVHLMDMVKPDKSFINKELEEKMELAQQITSMVLSL
                     RKSTKIKVRQPLNKIMIPALDPHFKEQIVAVEKLILSEVNVKSLEFMSDDSGMLTKKL
                     KPNFKTLGPKYGKQMKQLAAILTGFTTAQINDLEKEGICRFSIDGENIEVTLSDIEII
                     TQDIPGWLVASEGNLTVALDITLTDDLRDEGIARDLVNRIQNLRKEKDFEVTDKIHLK
                     IENKDIIASAIKKNFNYICSETLALSLNLVENINNNQSVEVELGEDIKIRVLVEKA"
     gene            complement(16112..16642)
                     /locus_tag="PK345_03680"
     CDS             complement(16112..16642)
                     /locus_tag="PK345_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34154.1"
                     /translation="MKDQQIALLRQQIAKLEDKNFDLKAWKAHTMIILASIFGEDSQK
                     VKEIGRLDYEFSSWSLRDATGHNTYQEGCKRLGKEIIEASINEIELTGLPQKRSATRG
                     MLDIQVILDALNDELKGSQYKNLLKIIKSNDLPEEKERLIKEIINEVGPEATRAILTN
                     MLLSDHFAEALSLKEK"
     gene            complement(17108..17335)
                     /locus_tag="PK345_03685"
     CDS             complement(17108..17335)
                     /locus_tag="PK345_03685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34155.1"
                     /translation="MKKYILTLIVTFVFALAGSGLMAQDPPPPPGNHGQTGNQPPGGG
                     APISSGVVILLTLSVAYGSKKVYNSWKKIGD"
     gene            complement(17374..20697)
                     /locus_tag="PK345_03690"
     CDS             complement(17374..20697)
                     /locus_tag="PK345_03690"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34156.1"
                     /translation="MKKNKLLKIFILMAGLMISSAGWGQTYLLDEDFSSITTGDNTTT
                     VGSNTKWNGNDNFPTVVNAFQAGGAVRIGTSKASGSITSDSLDLSVNGGSFKVSFDVK
                     GWTTIEGDIKVTVTGLTAQTVSYIAKMSDTFENKILTFTGGQANSTVKFETTAKRAFL
                     DNIKIYYESGPTITVDPSSLTGFTYVAGGGPSLEKSFTVSGINLTADISITASANYEI
                     STTSGSGYTSPITLIQNGGTVNDTTIYVRLKAGLNAGNYFNEDITASSTGAIDKTVTC
                     SGTVMPPPDPEPSNHVTGFTATTGDPTYSVINVAWVDAAGTQLPSGYLIKGSSVGYGD
                     IVAPVDGTPEADAPLVKNIPYGVQVASFTGLNENTTYYFKIYPYTNSGININYKTDGV
                     VPQDDATTEIAPPTINFDDNSKWVAGSVSLTSYASDHKYSDKNWIFTGGPGLRNTTSA
                     QDGFPGALGTYSWRLHTNTTVNWTATYTENLATNQNFTGFGFSARRWDNDPSPAYTVS
                     YSTDGGNKWTTATDIGSNGVLDNSAFGNSSDWKMFFQTIDTLLTGLEANKFIVKFSAT
                     TGERIMVDNFTYEITTEAPDSTTYTGTGNWANGSNWSNGMPGENTNVTIEGFVTVDDI
                     YTCNNFTIAPTGRVTIPETVNQQKGLWIKGDFLIRSDATGAGSFIGIYDQDLNWNYEV
                     QGTTTVERYISGASYAWHLISSPVSNQAIAGDWIPTSTGYDFYAWNEPTQTWLNQKVS
                     ANNITHFIPCKGYLVAYEETGATKTFTGTLNDGAQTLGLTVTGATSYYGSNLAGNPYP
                     SSIDWKGNNNAIDKSKLVGYNASEGVKHYIWNDASGNYGVYSDANAGDAGTNGANRYI
                     APMQGFFVSAASDGSFLINDSARVHSSQAWLKSGNNYEFRLCVNAPQGYGSDELLLEF
                     GHNVSVGSAEKWFSFVPTAPSIYSPIEDKKFSIRYLSIQDETLTIPVSFIAGVDGTYT
                     LTANFASSTYSAIKLEDKITGKVHDLLLTPEYSFSANKSDISERFLLHFTVTYGIENL
                     EENADIQIYSYGNSIYLNNNTDSETLVSVYNITGRQIYQDRVGAGSQHTFTINAPTGL
                     YIVKALTGKSITSRKVFIW"
BASE COUNT         5853 a         4149 c         4268 g         6476 t
ORIGIN      
        1 aagacagtta taatcaacaa ttatcgttta atttttagtg gacactaatg aaattttata
       61 atcatgaaga gattagcagg attaaaggct tgttgaaaaa gtagtgaaat cctattatga
      121 tgttaaatat taataattcc aaattgagca gtaacaaatc taaagataaa aatgactaag
      181 aattttaacg acaaaaaaat attttttgtg ggttcggggg ctatcgcaac agcgcttggc
      241 aatgtattgg taaaaactgc cgagcatgat gtaaccctta ttacgattga gaaagaggtg
      301 gcagattcaa taaaccgcga tcattacaac aataaatatt ttccggcaat aaggcttgaa
      361 aaaggcttga aagcatctgt tgattttagt ttgttgaaac aggaagcaat cgtatttatt
      421 gctatccctt cggctgttgt tgtgaatttt atttcgaatc gcgatattca tcccgactct
      481 attttggtaa accttgcaaa aggctttgga catcaacatt acaccattgt cgaatcttta
      541 aacaaattac ttccgaataa aatttgttcc ttaaaggggc cctcttttgc cagagagata
      601 atgaataatc aacctactgc aatgacctta ggcgctgaag atggaacatt atacccaata
      661 tttctggagt tatttaaaga gacattaatt tacaccgatt atactgacga tgtttatggc
      721 gttgagattt tgagcatact gaaaaatatt tatgctattg ctattggagt cgttgatgct
      781 cagtttgaat cgccaaacct gaaatacttg atttttacaa aagcgcttta tgagatgcgc
      841 aacctgctga tattgtttgg cggaaagaag aaaacccttt ttaactattg tggtgttggc
      901 gattttgctt taactgcact caacgacctc agccgaaaca gaacgctggg actgttgatc
      961 gggaaaggtt tttttactga caatatttcg gaaaaggtgg tactcgaagg caggatagct
     1021 gtaaatatcc tctataaccg attgttggag atgggattag atactcagga gtttccaatt
     1081 atctatgagt tatataaggt ttttaatggt acgtatgata tctcttcatt tgtaatcaat
     1141 ttattgaaaa acaatagata accacccgct ttgtttatat actatcaaga ataactgcca
     1201 attctttagt gaggtttttt cgggtataac tctcaatgtt tctgtattga aataattgtt
     1261 tttctgttaa aaaagcgtta tataatcgtt ccaaatggct ctctatcaat gtctggtcat
     1321 cataatctgc tatcaaaccc gagtctgtct gcctgatgat tgcagaggca tcgccatcag
     1381 caggaccgat gcagagaata ggtcgtcggg cagccaaata ttcaaaaagc ttccctgtaa
     1441 caatcatttt agcattttca gttcggttta tgatcaacaa taatactcca gcggcaactt
     1501 gctcttgaac aaccatatca tgaggaaggt aatcaatata ttccacatac ttttctatcc
     1561 cggcattgcg gatagcttct cttgcaaccc aatctgcttt tccaataatc tttatttcaa
     1621 gttcctgcgc aaactgctta ttctggttgg ttattttatt tagcgccagc catagagttt
     1681 ccggatttct gctttttgtc aatgatccaa tatgggctat tgtaaaccga ttgcttttta
     1741 tctgaatttc ttttggtata tcctcatcat caaatccatt ggtgatgaca tcgtaatggc
     1801 gcttgtgtat cgtttcgaaa gcagatttca tcccttgtga aacgacaacc actctatttg
     1861 ctttactaag cacctgtttt tcaagcttat gatgtttcct gtcggcaaag gcggtaagtt
     1921 taaggtcttt gtaataatca atatgtgtcc atggatcgcg aaaatcggcc acccacggta
     1981 agcctgtttt ttgatgcacc tttaaagcaa tcatgtgcat actgtgcggc ggaccggttg
     2041 aaataatagc atcaacagaa tataacgaga ggtattcgat taaatatttc accgaaggtt
     2101 ttatccaaaa cacacgggca tcgggaataa aaaaattgcc ccttatccac acggagattt
     2161 tttcggcaag agccggggtt ttcttttcag aaagaaaggc gctttttatc tgatcgctct
     2221 tttttcggcc tacaaatctt ttatataggt tataaggctc tttgattttg gtttttaaaa
     2281 cggtaatgtt gtcagctata tctttttcca acgaatggtc aacagcagga tattccgggt
     2341 tttcgggagt ataaatcact ggttcccagc cataatctcg caggtatttg gcaaacttta
     2401 gccagcgctg aacgccggcg ccaccactcg gaggccaata ataggtgatg atcagcgctt
     2461 ttttcattgt acagcgaaga tttatttctt tggcacgaaa atatctttta actcattacc
     2521 caatgcagca aagaaaaata gaattactat gagggaagtg atcagagata tttttcctcc
     2581 tacaaaaaag actttcggct caaatttcca gacgatctca tgattacctg aagaaaccat
     2641 cattgcacgt agcacgtaat tagctcggag atagggcatt ttcttaccgt caacatatac
     2701 attccaccct ttgtcgtaat atatctcgga aaaaacgacc aaattatcct ctgttgattg
     2761 atagttgtat atcagctcat taggcttgta actcattaat ttgatgcttc cgatcagagt
     2821 atctttattt tgaaatccgc taaccaactt ttcgaatctt cggtcaataa ttgcggtggt
     2881 tttaggatcg aagtcattta atgcctctat ctcttcatca gcattttcag cccattttat
     2941 attttgtatt aaccatgcat tgcctaacgc acgtggattt atgttggctt gtgctttttt
     3001 attctgatct tcaataatga aatacttcgt gttgagcatg ttgagtacat ctacattgtt
     3061 ttttgaaata tgatggtcaa tgagctcctg atagcgccgt agctttgcac catgataacc
     3121 tccgatagat ttatggaagt aggaggtgct gacatcgttg aatggactca cagtcagatt
     3181 gaatactctg aaattagggt ctttatcttc caagatcatc ttatcggctt cggatggggt
     3241 gtaggggatt gaaacttttt tctcatcaac aaaatcatcc tcatttaaat aacgcttatt
     3301 tacagcatac atatcaatcg taactaacac tataagcgct gcaaaaacaa actcattcct
     3361 tactttttta aataacccca tccagattgc gactattgca agtattataa aaaccaacga
     3421 acgtgtggcc tcggaggtta acatcgcttt tcggtcggcg cgtatggcat caattaccga
     3481 ctgtgggaaa ccagcttgaa tcagttgatt gtcaacttcg gatgagaaat tgaaaaggct
     3541 gccgccaaac actataaaaa agagtagtat cccaccgata acataaaaag ctgttataac
     3601 atgggaaaac atctttttcg gatcagtatt tttatcaaat atctctttca gggcaagtat
     3661 ggcgagtagt ggaatggtta ggtttgctat tacaagggtc atcgaaacgg ctctaaactt
     3721 gttgtaacca ggcacataac gaaggaaaaa ctcagtgagc ggcataaggt tatgccccca
     3781 tgatagcaat atagaaagga tagtggcagc aattagccac catttcattt ttccttttac
     3841 cacaatagct ccaagcgcaa ataaaaagac aataatagct ccggcataca cagggcctga
     3901 tgtaaaaggc tgatcgcccc agtacatcgg aatcattttt acataacttt ttgcctggtt
     3961 ggcaggtaag ccgagtttta caaattcttt ataagtattg gagtcctcgc ccggattgcg
     4021 cattgatcct cctcccataa aatcggggat aagcaaggtc atggtttctg cgcgaccata
     4081 gctccattgc gtggcgtaat ctatatcaag gcctgaagtc cggttttcgg gagtatgaga
     4141 aagttccgac gggccacgga tagtatattt gccatattcc cacgtagccc aaaggttaga
     4201 gatattaacg ctaaaagcaa atagtgcagc cgcaagtaac acaacagtag ctttgaaaaa
     4261 ttgtggaaac gtttgtgttt tgatgctctc aataagttgc acaataccaa aaatgataac
     4321 tatcaaaaga aggtaatagg tgatttgcag gtggttagcc attagttgaa gggataagaa
     4381 gagagccgtc atggctccac cccaaatata tttacctcta taggcgagca aaatgccggc
     4441 aagtaccggc gccatatagc caatagcatg agccttagaa ttatggccgg cttcgatgat
     4501 gataaaaaaa tatgaagaaa aagcaaaggc tattgctcct aaaagcgagt gccaaatgtt
     4561 tattttgagt gaaagaagta aaataaaaaa acccaagaaa taaagaaaaa caagcgatat
     4621 agggtagggg atgccgagtt gtaaaatgtt gtcaatatgc ttgacaagca ccttgttgta
     4681 tatcactgat atctgatagg cgggcattcc tccaaacatg gagtttgtcc agagcgcctc
     4741 ctcgcccgtt gcctcacggt aatccacaat ctctttcgac atcccgcgcc aacgctgaat
     4801 atcatcttga cggggtagct tcccatcgag aacaggcgag aaataaacaa gcattataat
     4861 gaggaaaaag acaatagctg ccgcatacgg ccaaatatgt tttatcgaaa acttatccat
     4921 aaaagttagg tataggaaaa ttatttttct ttaatctctt cgtattcaac atcctcaaca
     4981 gttttagggt tgaactttgt agaattggta tcggttgggt tgtaatttac agttacctca
     5041 ccttcaggac gagcggaatg gtgagcagac ccctgatgct ttgccgcttt tttaatgaat
     5101 gtctttaaaa gaaacggaaa taaatatctt gaaatgagct gaaaagcaag gacgaacaaa
     5161 aaaagaatgc cgagaaatct catttacagc aaattctgtt tttaatataa gtttgatgtt
     5221 gcgaaagtag ccaatttttg taaaatgaaa acagtcattt agtttgataa atattataat
     5281 tgccggaatg attataccaa aatcaaaaac ccgattagta atcgctttcg ttggattgcg
     5341 ggattgctta acaataaatt atcagtagta atgggtgttg attacaggct gccataattt
     5401 gcggtattta tcttcttcaa agcctttaca ggctgtttca aattcacatt tctgatgact
     5461 ctttaggctg atgatttggt tgaaggtaca tggtgatttt tcgcaaagat gatctttaca
     5521 aagtgttgta ttgttacagc agctcctacc caaagaccaa aggaagtcgt ttagtcggat
     5581 aagcggatgt ccggaatgaa tagctatcaa tttagcagct tcgatacaga ggcttcttat
     5641 cggctcgtct gttgtaacag ggtgtcgggc aataaggatt tgataaagcg ccggatcgct
     5701 aatctccaag catcccattc gcatcagcac acgctgcatg tgataatcca taatagggat
     5761 aaagttttca ggatcaccta tttcgagcag gccggaatct atcagcaact tgataaggaa
     5821 agtggatttt ttgcgttgcg gatcactgta ggcctctatc ttttgcaaaa cttcgtaaag
     5881 cccctttcca tcccttatca ggtaagcttt ggaatccgta atcaaaccgg taagttgacc
     5941 gccgcagatt gctataagac tttccgaagc atctttaatt aactgcgaac gttcttcaat
     6001 tctgtcaagg gtgcagtcat tactattttc attatgcgaa aaccatcttt gtaactcctt
     6061 tgcaatctgc tgagtagaaa gcagtgatac cttttcgggg ttgagcaagc tgctgttttg
     6121 ttgtgccatt ttgataaata catgctcgat gaaatcccat ccccaaaggt ctaacagcgg
     6181 atgatgtaat ttgtatgttt gatggcaaat agctactgca aaaaagttca tcctgagttt
     6241 gacctcatcc tttaatttta tcgtcaggta ttcgcgctca taaaaggtgg gatttggcct
     6301 gagattaccg atcacttctg caatttttaa acattgatcc cgattaaggt tagctatttg
     6361 tttcatcgta taggtcaaat gtgtttttat cgaacagaga atgattaagc aaaagcattt
     6421 ctgttactct tgaattccac tttcatggat tattattttg aaattattga tgatattgat
     6481 tttaatccag ccaagtcgtt catcatcaag cagtacgcct aaatagacat ttttctccaa
     6541 aggaaaattg ttacagttgt tgtgatcaat atcgtatctg taaattgttg tatctccact
     6601 tacaccaatc aacataggcc acgaattttt attgcctgag gtaagggtaa gtgaatcgca
     6661 gatggccggg tcggatttgc gtattttgtc atctctttct aaagggttta ttttaaaagt
     6721 cagattggtg ttaagtatcg tgtcgttgga gctgattctt atacaggaat agttgaacat
     6781 cagatacatg tcccatgtgc gaggttgtgc cccttcaaaa attagtgtat cgcgatttaa
     6841 atacattgta tccgttgtta caataccaag aacctttgca gagcaatgca aacaattgat
     6901 ggatgcttga ggtatttgcc ccattcccgg tgagccccat atccaactga caaactgaaa
     6961 atcgtctaaa ccattgttgt cgagatcaat attataggct ttttgactat aataaccacc
     7021 aatgagcgta gtatcataaa agttgattat catgcccgaa tgatacccag ccttgatttc
     7081 cggagcacgc tcaatagtac tcttagtaca ccctataagc tggataatga tgaaaagaaa
     7141 caagaatttt accgttcgat tactcatctt ttttttagca aagatagatt tttatccccc
     7201 tatcctttta gagtttgagt ccactttaaa aactttgttt acagaacgga caaggattta
     7261 attttaaaaa ttcggccttt gtttaaaatt tgttttaaaa gtattaacag gattgatgtc
     7321 aaagcagata cctattgact aaaatacctc ttcggagata atatctttat ttaaaaatag
     7381 tgtaacactc cttgtacccc atagttaatg ccttttttaa acagattaaa ctaaatgata
     7441 aacaaatttg gcttcgtaac aatcaatgta caacagtttt ttcgttagtt ttttagcgtt
     7501 tttcaaattt ctatccggta ggcatataaa agggtcttta taatttgtaa ttttacagcg
     7561 tttatttttg agaagtaaaa aatgaaggtt gtttcaagag ttgaagatgt tattaaccca
     7621 tcaatagttg agcggggtag tattatttgt acagctggcg ctgatgttac ttcacaggtt
     7681 ttgttacgtt ctcttgcagc cgatcctgta aattttgtta cagaaaatgg cgctgtcaac
     7741 ccgcaaggcc ttaatattgc cgaacatgcg gtttccatat cgcatctcgc cgcttcggaa
     7801 caccggaaaa aacttctcaa atacttttac gaaagcaagg agtatcatga gccgccggaa
     7861 gcatttggaa aattatccct aagggcttta cactttatga ggatattgct actcccattt
     7921 tttccaatct ttagtgaaaa ttattaatta ttaaaatata aataaaatga agtctttaaa
     7981 attatctcta tggttaacat tacttattat gatcagtttt tcgtgtgcct gtcctgatcg
     8041 gcctgacaac ggcgaagacc aacgcgcagg tattatcccg caagatgtgg agtttgaggc
     8101 gctgcctgac cttgatatgg aggtcaagct tgctgagttt gctgagtttg aacttactag
     8161 caacttagat cacctcacag ttgatgaacg tgcaatattg ccgatacttt ttgaggcagc
     8221 ttcaataatg gaaaatatct tttggaaaca ggcaataggg aacaaagagg atttcctaaa
     8281 caggataaaa gatgaaaagg cgagaatgtt tgcccaaatt cattatggcc cgtgggatag
     8341 gatgaataac aacgagcctt ttcttacaaa tatcggccta aaacccgaag gtgcaaactt
     8401 ttaccccgac gatatgacca aggaggagtt cgaaaatttt accgatcccg ataaaacaag
     8461 tcaatatacg cttattcgaa gagatgaaga tggtaattta aaggtactgt ggtatcatga
     8521 agcctataga gtgcaaattg aaaaggcggt tgatttgtta agaaaagcag cggaaatatc
     8581 cgaaaatgac ggttttaaaa attaccttaa tttaagagca gaggccttac taaccgatga
     8641 ttataaggcc agcgacatgg catggatgga tatgaaaaat tccaatatag attttatcgt
     8701 cgggccaatc gaaaattacg aagatcaaat gtttggctgt aaagctgcct ttgagtcttt
     8761 tatattgatc aaagaccccg attggagtag tcgtttacag cgttttgccg ccctacttcc
     8821 ttcactccag acgatgatcc cgaccaacga aaaatacctg aaggatgcgc ccggttccga
     8881 ttccgacctt aacgtgtatg atgccatatt ttatgccggt gattgtaatg caggtagtaa
     8941 aacgatcgca attaatcttc ctaacgatcc ggaggtgcac gtagcaaaag gaacacgcaa
     9001 gttacaactg aaaaattcga tgaaagcaaa atttgataag atattgatac cgatcgctaa
     9061 gatattaata gaccccgtgc agcaaaagca tgtaacgttt gatgcctttt ttgcaaatac
     9121 catgttccac gaagtagccc acggaatggg ggtaaagaac accgttaccg gtaaaggaag
     9181 cgttcgtgaa gcgctaaaag agacatatac ctccattgag gagggtaaag ccgatataat
     9241 ggggctgtgg cttgtttcaa gattaaggga aatgggtgag atatccgatg gcgagctgat
     9301 ggacaacctt gtaacttttt cggcaggcct gttccgttca agtcgtttcg gaacggcaag
     9361 cgctcatgga aaggctaaca tgatgagatt taactttttt gaagaacatg gcgcttttac
     9421 ccgaagcgat aaaggctttt atactgtaaa tatggaagag atgttggaag caatgaatat
     9481 ccttgttgac aaaattatga ctattcaggg cgaaggggat tatgaagctg caaaggcatg
     9541 gattgaggcc gacggcaatg tgagcgagag cctggaatac gacttgaatc gcctgaatga
     9601 ttatggaata cctgtggata tctattttaa acaaggccct gatgtggcgg ggattaaata
     9661 gaacttcagt tcaccgtttg gcaacgtgat attcacggtg aaaaaactgg atcattataa
     9721 ctttgtttgg ggctaaatca tgttttccgt tttttttgtt ttgcagccgg aaacaggaca
     9781 ttgtttaaaa tcaatctata gccgggcgag ttgggatgta gattgaggtc tgtcggcggg
     9841 ttgcctacaa ggtgccggta atcttcaggg tcgtgtccac cgtaaaaagt aaaggtgcca
     9901 tgaccgtatt cgccgtgtat gtagcgtgcc tcattggcgg ccttgtactc acccattata
     9961 agtacgttgg gttttatggt gttcatgttg aaagcggtgg tttgacccat aaatcctttt
    10021 attacctgca tatgattctg gcaaagcatt gttggcacag gatcccactt ggccgaaaaa
    10081 tcaaatagcg taaaaaagtc attcttttcg ttgatcgtgc gcggacgagt ttcagtaaca
    10141 tcaatatcgg atatctcgta aatgttagga ttggtaacaa gtgtaaagtt ggtaaatgca
    10201 agcgtgttat caaaattaag cttcgactgg gcattagggt ccatcggatc gccgtcgaac
    10261 attgattcaa cgatatcaac gccatcggca gctaaggcga tatcgaaact gtcgggcgcc
    10321 gaacacatag caaacatata accgccgcct gccacaaaat ctcgaatctt ttttgcaact
    10381 gcaagcttca tctgcgatac cttggaatat ccgagtaacc ttgccatctc ttcattcacc
    10441 cttacatctt cctgatacca tgcagcatta cgaaacgatc tccagaattt accatattgc
    10501 ccggtaaaat cctcatgatg caagtgtagc cagtcgtaca taggaagtac gccctccatc
    10561 acctctaaat cataaacaac gtcgtaagga atttcggcat aagtgagcgc caatgtaact
    10621 gcatcgtccc acggaagctt atgctttggc gagtaaacag caatcttagg ggctttgtgc
    10681 aatttaatct catccatatt ctcttccgga tcggaaatct cgcgaataat ttgtgcagcc
    10741 tggatgtcgg caataatctc aaaactaaca cctctgacct ttaattcgcg ctctacctgc
    10801 tcataataac ggcacataaa actaccccca cgatagttga gcagccagct tacttccacc
    10861 tcacgctcta aagtccaata ggcaatacca taagctttta aatggttttt ttgactattg
    10921 tccatgggga ttaaaagata ggccgcatgg cttaaaagaa aagaaaagag aaatgttata
    10981 gtgaatatta attttctcat agacaaattt tgatgatgtt ttgaataata atgggtttca
    11041 gcttttttta ttgctccaat gataatacaa ttttaagcct gttgtcgtgt aaacttcact
    11101 ttaaattaaa taagctctgg ttgagttgtt taaccttatt aaaccctatt tattaataac
    11161 ttaaatagct tgctaaaatc ttagtttacg accatgccaa ttctttttta ttcaattttt
    11221 gcttattcct gaaaatcttt aaatggctaa ttcaggatta tgatttgtct ttcaatcccg
    11281 atactctcat cagccaacgc attagtcatt agttcctatt ttcaagtggt cagattgggt
    11341 ttgaactatc actttcggta ttaaggtttt catcttcttc gatttgaaga gtttcacttt
    11401 ctgttggttt gtttttacgg aatatcttac cttgaaaaat gattaaaatg aaaacgccga
    11461 tagtgatgga tgaatcagca atattgaata ccggcctaaa aaatataaaa tcgtcgccgc
    11521 ccactatagg tacccatgca gggtactcgc tttgaataat agggaagtaa agcatatcaa
    11581 ccacttttcc gtggaggaag gagccataac cgccttccgg aggaaaaagc tttgcaactg
    11641 tagtatatga tgagttgcta aagatcaatc cgtaaaatgc gctgtcgagc atattgccga
    11701 tagctcctgc cagaataagt gatatgctga cgatcagcgc tttatgatat tttttgcgga
    11761 taatttcgcg gagataaaaa aagataatgg ttatggccgc tatccgaaag aggcttagcg
    11821 ccaattttcc gaatattccg ccaaaggaga ggccaaaggc cattccttca ttttcggtaa
    11881 aatggatgat gaaccagttg ccaaggatgt tgatctcctc tcccaaggtc atgttcgttt
    11941 taatcccgaa ctttaaaacc tggtcagcaa tcagtataag cgttattata aggagtgatt
    12001 ttttccacac ggcaacgtaa tggttttaaa ataataaaag tggttatccc tatttttgca
    12061 taaggattcc gtaaaaagtt ctgagatgcc tcagtaccat ggtggtatga gctttatttg
    12121 cgggtctgat attgttttag cttggcctcc atgctcaatg tggcgtgggg tacgctttta
    12181 agcctctctt tagcaattaa cttacctgtt acccgacaaa ttccgtaagt accgttttca
    12241 atgcggataa gcgcattctc caagtttgtt ataaactttt gctgacgaac ggctaaccgg
    12301 ctgttctcct ccttcgaaag cacattatag ccctcttcta atactttaaa ggttggggag
    12361 gtatcgttgg tgtcgttttc gttaacattg ctatatgctt ctgtcagcaa ggccagatcg
    12421 gcacgagcct tttcaagctt ctcaagaata atctgacgaa actcctctaa ttcttctttg
    12481 ttgtaccttg tcttgacaac ttcgggagca ttgctgtcca tactttgatt tgccatagtt
    12541 ctctgtttta ataattaata ctccttttat ttttccctgt ttgattaagc cttttcaacc
    12601 aataccctta ttttgatatc ctcgcccagt tctacttcaa cgctttggtt gttatttatg
    12661 ttttcaacaa ggttgagcga aagtgcaagt gtttcggagc aaatatagtt gaaattcttt
    12721 ttgatagccg atgctattat atctttattt tcaattttca gatggatctt atcagttact
    12781 tcaaaatcct tctcttttct taagttttgt attctgttta ccaggtcgcg ggcgatgcct
    12841 tcatctctga gatcgtcggt gagtgtaatg tcgagagcca ctgtaagatt accctctgag
    12901 gctacaagcc aaccggggat atcttgagtt atgatctcta tgtcggatag cgtaacttca
    12961 atattttcgc catcaatgct aaacctgcaa atgccctctt tttccagatc attgatttgt
    13021 gcagtggtga atccggtaag aatagcggct aattgcttca tctgtttacc atatttaggc
    13081 ccgagtgttt tgaagttggg ttttaatttt ttggtcaaca ttccggaatc atcgctcata
    13141 aattcaagcg atttaacatt tacttcgctc agtatcagtt tttcgacagc tacaatctgt
    13201 tctttaaagt gtgggtcgag cgccggtatc ataattttat tcaagggctg gcgcacttta
    13261 atttttgtac ttttcctcaa cgacagcacc attgaggtga tctgttgcgc caactccatc
    13321 ttctcttcca actctttatt tataaacgac ttatcgggtt ttaccatatc catcaagtgc
    13381 actgaatcca catcgtatcg tttggtaacg ctgttgaggt cgaggaatag cttttcggag
    13441 aagaagggag ctatcggcga aatcatcact gcaatagatt caaggcactt gtaaagggtt
    13501 tgataagctg ttatcttgtc ggttgacatc tctcctttcc agaaacgccg ccttgagagg
    13561 cgcacgtacc agttgctaag cttataatct acaaaatgag ctattgcgcg ccctgctttt
    13621 gttggttcgt agtcatcgta agcctcatcc actattgtga taagtgtgtt gagctccgat
    13681 aaaatccagc gatcgagttc cgtacgctct tcgagtggca cctcttgctg gttgtagcgg
    13741 aaattatcaa tatttgcata aagcgcaaag aaggcgtaag tgttgtatag tgtcccgaaa
    13801 aacttacgct gaacctctat gatgccgtca gtatcaaatt taaggttgtc ccatggctgt
    13861 gcgtttgtga tcatgtacca ccgtgtggcg tcggctccaa attttttaat ggtctcgaaa
    13921 ggatctacag cattgccaag gcgcttcgac atcttattgc catttttatc gagaaccaag
    13981 ccattggaaa cacaggtttt gaaagctacg gagtcgaaaa ccattgttgc tatggcatgc
    14041 agagtaaaaa accagccgcg cgtttggtca acgccttcag caataaaatc ggcagggaaa
    14101 tagtcggcaa ggtgatcgcc tttttcgaaa ggataatgaa actgagcata aggcattgcg
    14161 cccgaatcga accagacatc aatcaggtcg agttcgcgat acatcttctc tcccttatcc
    14221 gaaacgagta ttatctcatc aatatagggt cggtggagat caaatttgga gtaattctcc
    14281 tccgaattat catctgatat atattctgca agcgggttat ttgtcataaa tcctgcttca
    14341 acagcgcgtt caatctcctc ttttagttgc gctaccgagc cgatacactt ctgttctttc
    14401 cggtcttcgg taatccatat cggcaggcct acaccccaaa accgtgatct ggagaggttc
    14461 cagtcaacaa ggttctctaa ccagttgcca aacctgccaa caccggtaga tttaggtttc
    14521 cagttgatgg tgttgttcag ctctatcatc ctctctttaa aagcagttgt tttgataaac
    14581 cacgagtcca acgggtaata gagtaccggc ttatctgttc gccagcaatg gggataatta
    14641 tgtacgtatt tctctatttt gaaggcttta ttgtctaact ttagcattac tgagaggtca
    14701 acgtcaagta tcggcgtttc aggcgtagct tccaggtcgt agtcattctt tacatatctg
    14761 cctgcatatt cactgtagag gcttacgtcc acgaattttt tcacaaactc gttgtcgagg
    14821 tcttcaatcc tgaaaaagcg gcctttcagg tcaaccatcg ggcgcttgct tccatttttg
    14881 tcaataagta tcagtggcgc tatatccgag gaacgtgcaa cacggtcgtc gtcggcgcca
    14941 aaagtaggcg caatatgcac tacgccggta ccatcttcag tagtaacaaa atcgcctaag
    15001 atcaccctaa aggcatcgcc atcgggtttt atccatggca taagctgctc gtagcggact
    15061 ccctcaagca gggctccgtc aaattcgtcc aatatccggt aggggataag cttatcgcca
    15121 attttgtaat cttccaacgg caggtcggca gccttttcgt tgaagtagct atttagtaag
    15181 tctttagcca aaatcacggt tatctctttg ccggtatatt ggttaaaact ttgcactttc
    15241 agatatctga ttttcgatcc tactgccagc gcggtgtttg acggcagtgt ccaaggggtg
    15301 gtagtccagg caagtataga aacctgatcg gttttgttgc taaagaggaa ttttgatttc
    15361 tcgtttctga taacgctaaa gagggcagtt acggatgtat cttttacgtc gcggtagcag
    15421 cccggctggt taagctcatg tgcgctaagg cctgatcctg ctgccggcga atagggctga
    15481 atggtgtatc ccttgtagag caaccctttt tcgtaaagtt tcgaaagcag ataccataca
    15541 ctctctatat atttgttttc gaaggtgata tagggatcgt taaggtcaac ccagtagccc
    15601 atttttattg ttagctcgtc ccataaatcc ttgtatttca tcacctcggt acggcaggcg
    15661 cggttgtact cttcaacact gatgcttttt ccaatatcct ctttggtgat ccccaaagtt
    15721 ttttcaacgg caatctcgat aggcagaccg tgtgtgtccc atcctgcctt gcgtgcaacg
    15781 tagtatccct tcaaggtttt ataacgacag aagatatctt ttatagtacg ggagatcatg
    15841 tgatgtatcc ccggtattcc gtttgctgag ggagggcctt cgtaaaaaac aaaactctca
    15901 gagccttcgc ggttctttaa acttttatga aaaatatcat gctccttcca ataggcaagg
    15961 atttcctgat caatacggat taggtttaaa ttgtcgtatt ctttgtattt ggaaaccatt
    16021 gaataatctg ttaggccttt taaaaattgg gcgcaaaatt aataaaaatc tgttgggtga
    16081 gcgctttctt ttatcctctt taatgtatcg tttacttttc ttttaatgat aacgcttccg
    16141 caaagtgatc gctgagaagc atatttgtga gtattgctcg cgtagcttcc ggcccaactt
    16201 cgttgatgat ctcttttatc aatcgctctt tttcttcagg cagatcgtta cttttaatta
    16261 ttttaagcag atttttatat tgcgatccct tcagttcatc attcagggca tcgaggatca
    16321 cttggatatc caacatgccg cgtgtggccg atctcttctg tggaaggcct gtaagttcaa
    16381 tttcattgat tgaggcctct attatctctt tcccgagcct tttgcacccc tcctgatagg
    16441 tgttgtggcc ggtagcatcg cgaagcgacc aactgctgaa ctcgtaatca agacgcccga
    16501 tctcttttac tttttgactg tcctctccaa agatagaggc taaaatgatc attgtatggg
    16561 ctttccaagc ttttaaatcg aagtttttat cttcgagctt tgcaatttgt tgcctcagca
    16621 gagctatttg ttgatctttc atgcttaatt aggttcgaag gtgtatattt tagtttaaga
    16681 tttatcggtt caaaaagtcc atcaaagata caaaggactt tttaataggc atggcgcaac
    16741 actctatccc gctaagggta agcgatcggc aaaaggggta tcaaagtttc ggagatttgc
    16801 acggagtttc acagagtttt tgtttcctct gtgttacttt ttttcttcca cttattaaac
    16861 gctcctgctt ttgtcatttc ggtgcgggct atctttccac atacatcttt ctttactgat
    16921 gcttcgtacc tgacggcgcg gctttacctt ttccggcctc ttttttatct taaactactc
    16981 taaaaagcct ttgcaggaga gtatcgttaa gtttgtcgtt tagagtatgc aacagtggtt
    17041 ggaaattaat aaccccgcca acggttgacg gggttataat tgaaattcgc gcattggtca
    17101 aagcaagcta atcgcctatc tttttccacg agttatacac ttttttactt ccgtatgcaa
    17161 cgctaagggt aagcagaata accacccctg agctaattgg agcgccgccg ccagggggtt
    17221 gattccccgt ttggccatga tttccgggag gcggtggcgg atcttgtgcc ataagcccgc
    17281 tgccggccaa agcaaaaaca aaggtaacga ttaaggttaa gatgtacttt ttcatagtgg
    17341 tatgttgttt aagtttaatt attgactaat tgattaccaa ataaatactt tacgacttgt
    17401 aatggatttg ccggtaagcg cctttacgat gtacaagcct gtaggcgcat ttattgtaaa
    17461 agtatgttgt gagccggcgc caacccggtc ttgataaatc tgccggccgg ttatgttata
    17521 aaccgaaact aacgtttcgc tatctgtatt attattcaga taaatgctgt tgccgtagct
    17581 atagatttgt atgtcggcat tctcctctaa attttctatg ccgtaggtta cggtgaaatg
    17641 taataaaaag cgttctgaaa tatcggactt gttggcgcta aacgaatact caggcgtgag
    17701 caagagatcg tgtactttgc ctgtgatctt atcttcgagt ttaattgccg aataggttga
    17761 ggaggcgaaa ttagcagtta aagtatatgt tccatctacg ccggcgataa atgataccgg
    17821 aatggtcaat gtttcgtctt gtatgctgag gtatcggata gagaattttt tgtcttctat
    17881 cggggagtag atactggggg cggtaggaac aaagctgaac catttttcgg cgctgccaac
    17941 cgatacatta tggccaaact caagcagcaa ctcatcggag ccataacctt gcggcgcatt
    18001 tacgcagagg cggaactcat agttattgcc cgatttaagc catgcctgac tgctgtgtac
    18061 acgagcgctg tcatttatta agaaagaacc atcactcgcg gcgctcacaa agaatccctg
    18121 catgggcgct atataacggt tagcgccatt agtgcccgca tcgcctgcat ttgcatcact
    18181 ataaacgccg tagttgcccg atgcatcatt ccagatatag tgcttaacgc cttcggatgc
    18241 attataacca accaatttac ttttatctat cgcattatta ttacccttcc agtcaataga
    18301 tgagggatag ggattgcctg ccagatttga gccataatag cttgtcgcgc cggttactgt
    18361 taaaccgagc gtttgagcgc catcatttaa cgttccggta aaggttttag tagcgcctgt
    18421 ttcctcataa gctactaaat atcctttgca gggaatgaaa tgtgtaatat tgtttgcgct
    18481 aactttttgg ttaagccagg tttgtgttgg ttcattccag gcgtaaaagt cgtagccggt
    18541 acttgtgggt atccaatcac ctgcgatagc ttgattgctt accggcgaag atattagatg
    18601 ccatgcataa gatgcgccgg agatataacg ctcaacggtt gttgtgcctt gaacctcgta
    18661 attccaatta aggtcttgat cataaattcc gataaacgac ccggcgccgg tagcatcgga
    18721 ccggataagg aaatctcctt ttatccataa tcccttctgt tgatttacgg tttcaggaat
    18781 tgttactcta cccgtagggg caattgtgaa attgttgcat gtgtaaatat catcaaccgt
    18841 tacaaagcct tcaattgtaa cattcgtatt ttcgccgggc atgccgttgc tccagttaga
    18901 gccatttgcc cagttgccgg taccggtgta ggtagtcgaa tcaggcgctt cggtagttat
    18961 ttcataagta aaattatcaa ccataatacg ctcgccagtg gtagcagaaa acttaacaat
    19021 gaatttattt gcttcaaggc ctgttaataa ggtatctatt gtttgaaaga acattttcca
    19081 atctgaagaa tttccaaagg cgctattgtc taaaacaccg tttgagccaa tatctgttgc
    19141 agttgtccat ttgtttccac catcagtact ataagaaaca gtatatgcag gtgaaggatc
    19201 attatcccat ctacgtgcag agaagccaaa acctgtaaaa ttctgattgg tagctaaatt
    19261 ttctgtataa gtagctgtcc aatttactgt agtgttggta tgcaatctcc acgaataagt
    19321 acccaaagcg cccggaaagc catcttgagc tgaagttgta tttcttaaac caggtccacc
    19381 agtgaaaatc caatttttat ctgaatattt atggtcggag gcataggaag ttaatgatac
    19441 tgagcctgct acccattttg aattatcatc aaagttgata gttggcggag ctatttcagt
    19501 tgttgcatca tcctgcggca caacaccatc ggttttgtaa ttaatattaa taccgctgtt
    19561 ggtgtagggg taaatcttaa agtaataggt ggtattttca tttaaaccgg taaatgaagc
    19621 tacttgtact ccgtaaggaa tattttttac aagcggagca tctgcttccg gtgtgccatc
    19681 aacgggagct acaatgtcgc catagccaac gctacttcct ttaatcagat atccggaggg
    19741 gagctgggta cccgctgcat caacccatgc cacattaata acagagtatg taggatcgcc
    19801 ggtagttgct gtaaagccgg taacatggtt gcttggctcg gggtcgggtg gcggcattac
    19861 agttccgctg caggtaaccg ttttatcaat agctcctgta gaagaagcgg ttatatcttc
    19921 gttgaagtag tttcctgcgt tcagcccggc tttaaggcga acataaattg ttgtatcatt
    19981 aaccgttcca ccattttgga taagcgtaat tggcgaggta tagccagaac ccgaggttgt
    20041 ggaaatctca taatttgcgg atgccgttat gcttatatct gcagtaagat ttatgccgct
    20101 taccgtaaag cttttctcta gcgaaggacc gccaccggct acataagtaa agccggtaag
    20161 ggaggaggga tcaacggtga tggtagggcc agattcataa tatattttta tattatctaa
    20221 aaatgctctt ttagcagtag tttcaaattt aacagtagaa tttgcttggc cacccgtaaa
    20281 tgtaagtatt ttgttttcaa aagtatcaga catttttgca atataagaaa ctgtttgagc
    20341 tgtcaatccg gttacagtaa ctttgatatc gccttcaata gtggtccatc cttttacatc
    20401 aaaactaact ttaaatgaac ctccgtttac acttagatca agactatcgc ttgttattga
    20461 cccacttgcc ttgctagttc caattcttac agcgcctccg gcctggaagg cattgacaac
    20521 agttgggaaa ttgtcatttc cattccattt tgtatttgat ccaactgtag ttgtgttatc
    20581 acctgttgta atagaagaaa aatcttcatc taaaagatac gtttgccccc atcctgcaga
    20641 gcttatcatc agccctgcca taaggataaa aatctttaga agtttgtttt ttttcataat
    20701 tttttaaaat ttaagtttat taattatttc ttttttttct ttatct
//
LOCUS       DAOOAI010000032        20631 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_3409_length_20631_cov_6.054918, whole genome shotgun sequence.
ACCESSION   DAOOAI010000032 DAOOAI010000000
VERSION     DAOOAI010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20631)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20631)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20631
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3409_length_20631_cov_6.054918"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="PK345_03695"
     CDS             <1..190
                     /locus_tag="PK345_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HPX34157.1"
                     /translation="HRVALWEKHGCIAIGKSPEKAFDLIDVLEKSAFIFFIYKNAGYN
                     VEGINDIKQKKMRRHFKI"
     gene            258..1439
                     /locus_tag="PK345_03700"
     CDS             258..1439
                     /locus_tag="PK345_03700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34158.1"
                     /translation="MKNYTLIVVSDPSSESEFLDFPSRLYKSDPNYIRPLDEEVKAIF
                     DPAKNKLFRNGDAKRWLLKDDNNKTVGRIASFYNEALSRANDQPTGGIGFFDSINDVE
                     ASKILFDASREWLVGKGMEAMDGPINFGERDSFWGCLVDGFYPPIFNMPYNFPYYKDL
                     FESYGFQNYFNQYTYHRLLVPGKLTTVVEEKAMKILNNKDYHFKTIDTKNTDKFIEDF
                     MIIYNKAWARFPGIKKLSKAHAAALFKSLKPIMDPRLILYGYYKNEPVAFFIMMPDIN
                     QIIREFDGKLNMINKLKLIFKIKYTSATDKVIGRIFGVVPEHQGRGVEAGLIKTFEKI
                     VIKPKFHYKDLEMNWIGDFNPIMMKVCEQIGATILKTHITYRYLFDRSLPFKRAKRVN
                     V"
     gene            1441..1905
                     /locus_tag="PK345_03705"
     CDS             1441..1905
                     /locus_tag="PK345_03705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34159.1"
                     /translation="MEFLLAVDLGINTGLALFGSDGRLYWFRSKNFGSASRLRRAIPW
                     LLNQEEDITTVIIEGGGPLLKIWDAEFNKRNLTVLRIMADQWRQEIFYQREYRNRKDA
                     KENAKYYATKVIEKLSESGLTSLNENTAEAILIGLYGMKMLNWIDSINKTLR"
     gene            complement(1927..2520)
                     /locus_tag="PK345_03710"
     CDS             complement(1927..2520)
                     /locus_tag="PK345_03710"
                     /inference="COORDINATES: protein motif:HMM:NF027788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPX34160.1"
                     /translation="MPYLVLDLEMTGPEPDYNEIIQIGAEIYDDKWKLMGEFISNVYP
                     ENEDAFSSHSEEIHGISIDELEDAPMIYEVLADFEKWIRKCLFYREQDSLKGVVICGQ
                     SIINDINFLSFAYKKEKLAWPFSFRMLELHSVSQYAFMILENNGKKIPRSLSLENVAS
                     YFGFSRDGDTHNALEDARLTAKCFIALNDLAKNVILK"
     gene            complement(2636..3082)
                     /locus_tag="PK345_03715"
     CDS             complement(2636..3082)
                     /locus_tag="PK345_03715"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HPX34161.1"
                     /translation="MFNFNTHEYYMKEALKEAYEAFTKDEVPVGAVIVCNNRIIARAH
                     NLTEQLTDVTAHAEMQAFTAASNYTGNKYLDECTLYVTLEPCVMCAGAAYWTQISKIV
                     FGAFDHKKGFTLINKNILHPKTELITGILQNECGELLKRYFKEKRT"
     gene            3210..3701
                     /locus_tag="PK345_03720"
     CDS             3210..3701
                     /locus_tag="PK345_03720"
                     /inference="COORDINATES: protein motif:HMM:NF012436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HPX34162.1"
                     /translation="MISKKIESAINDQILLEELSSRVYLAMASWCEVQGLQGVANFLY
                     KHSDEERMHQLKLIRYLNDREGYAELQALPKPEIKCKDIEELFTQVLKHEQLVSKKIN
                     DLVDMSLEEKDHATFNFLQWFVSEQVEEESLVNNILAKIKLAGDSKMGKFHIDNFLAS
                     LDD"
     gene            complement(3782..5419)
                     /locus_tag="PK345_03725"
     CDS             complement(3782..5419)
                     /locus_tag="PK345_03725"
                     /inference="COORDINATES: protein motif:HMM:NF015666.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP binding domain-containing protein"
                     /protein_id="HPX34163.1"
                     /translation="MENYFSISPHGQQFPLPEKNEYPAEFNRIKVLADKARSEGKEIV
                     VVMGVGFVGAVMAAIIADTKDENGNDSKFVIGCQRPSVRSYWKIPILNQGISPVKAED
                     PEVDILISRCVKEKKTLIATYNSEVLKLADVVVVDVQCDYEKRELGNMRNGEADMAAL
                     EATMRTIGETIMPHCLVLIETTVAPGTTEFVAWPILKKAFAKRGLKETPLLSHSFERV
                     MPGKEYVSSIRDFWRVCSGCDIEARKRVEKFLHEVINTKDFPLTVMDRPIESETTKII
                     ENSYRATILAYLHEWSIFAERNGVDLIKVINAIKMRPTHSNMIFPGPGIGGYCLPKDG
                     GLGYWSYRHILGFEDGESLFKITPTAIDINDTRGLHVAQLVRDALRNMARYIAGSQVL
                     ICGASYRQDVGDTRYSGSEMVVRKLTEMGAEIRVHDPYVDHWYELENQDTYPSPKSSW
                     KRFFRNQEHLKDIRVGKDLKKSVNGVEALIFAVPHSEYLDLNPDDIVSWAGAPIAVID
                     AFGMLSDEKIKRYFELGCEVKALGRGHVQRIKQEIRG"
     gene            complement(5467..7926)
                     /gene="pheT"
                     /locus_tag="PK345_03730"
     CDS             complement(5467..7926)
                     /gene="pheT"
                     /locus_tag="PK345_03730"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HPX34164.1"
                     /translation="MKISYNWLKEYINIDLTPEEIAPILTNTGLEVESIEKFESIKGG
                     LKGVVIGEVKTCEKHPGADKLSITTVEVGQGNILPIVCGAPNVAAGQKVLVATVGTMI
                     HMKDKLFEIKKTTIRGEVSEGMICAEDELGLGNSHEGIVVLPDDAEVGLPANEYFSVE
                     EDYVFEIGLTPNRSDAMSHIGVARDLAAALNLQKKCDLIKLIKPNDSAFRPDNNDYPI
                     SVIIEDAKACPRYSGVTITGVEVKESPEWLKNRLKAIGLRPINNLVDISNFILHETGH
                     PNHFFDADKITGKKIIVKKEPKGTKFTTLDEVKRELSGEDLMICDEEKGMCMAGVFGG
                     LYAGVSESTKNIFIESAHFDSKTIRKTARYHGLNTDSSFRFERGTDPNATIYALKLAA
                     LMVKRIAGGTISSDIVDVYPNPIEKKIVELKYENVDCLIGQFIPRETIKDILIWLEME
                     IIDETDNSLMVEVPTFKADVDREADIIEDILRIYGYNNIAIPSQIRSSISYTKKPDRE
                     YLQNIISDFLSGNGYYEIMCNSLTKSEYADKLLIYETENNVKIQNPLSSDLNVMRQSL
                     VTSGFEVISHNLKHKNLDLKLFEFGNVYSLIKTKPGAHPLKKYKENTHLTIFITGKTN
                     NESWYQQQNEADFFFLKNTVFNILKRLNLNEKRFKNENISNELFESGIRMFLNDKSTI
                     EFGVVNRRILNYFEIKQSVIMADLHWDNIIHEMRNHKITYKPIPKFPEVRRDLALVVD
                     QNIEFETLKTLAFEAEKDLLKEVILFDVYEGEKIVKGKKSYAIGFFLQDDHKTLTDKT
                     IEKTMAKIFALIQKQTNALLR"
     gene            8130..10094
                     /gene="gyrB"
                     /locus_tag="PK345_03735"
     CDS             8130..10094
                     /gene="gyrB"
                     /locus_tag="PK345_03735"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HPX34165.1"
                     /translation="MTAEEKNLIATKEYSAENIQVLEGLEAVRKRPSMYIGDTGARGL
                     HHLVYEVVDNSIDEALAGYCDKIEVVIHEDESITVKDNGRGIPVGMHEKENRSALEVV
                     LTVLHAGGKFDKGSYKVSGGLHGVGVSVVNALSKVLKASIYREGKLFYQEYNYGKPLE
                     PVKAIAETKDTGTEITFSPDPTIFPEIKFDYNILATRLRELSFLNKTISLSLTDERLT
                     DEDGNFVSEEFYSENGLLDFINYLDETREKLIPEAIYIEGEKNNTPIEIAMQYNTSFS
                     ENLHSYVNNINTHEGGTHLTGFRRGLTRTLKAYAEKSGLLAKLKFDINGDDFREGLTA
                     IISVKVAEPQFEGQTKTKLGNSDVMGSVDQMVSELLTNYLEENPREAKTIVNKVILAA
                     TARHAARKARELVQRKNVLTGSGLPGKLADCSERDPELCEIYLVEGDSAGGTAKAGRD
                     RRYQAILPLRGKILNVEKAMQHKIFESEEIKNIFTALGVTIGTDEDSKALNIEKLRYN
                     KVIIMTDADVDGSHIATLILTFFFRYMKELIEMGNIYIATPPLYLIKKGKDERYCWTE
                     EERNKIVTKWSVNGDAKGIHIQRYKGLGEMNAEQLWDTTMNPQNRILRQVTIENGTEA
                     DRIFSMLMGDEVPPRREFIEKNARYAKIDI"
     gene            10115..11182
                     /locus_tag="PK345_03740"
     CDS             10115..11182
                     /locus_tag="PK345_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34166.1"
                     /translation="MKLIIRSIIVFTIILVFILLIFLLFSTLDEYKPSNKEILHPETF
                     SFSKTLSDSIISLMTWNIGYASMGSETDFFYDGGKMVRPNRELHEKYLDSIVNFFQPE
                     KAPNFILLQEVDYEAKRSYFSNEKETLQRRLIDYSSVFAVNYNVFFVPVPLFNPMGKV
                     IAGMISFSDVEPKSSFRIAFAGNYPWPVRLFNLKRCFILQRFNTQSGKELILINIHNS
                     AFDKGDLRKIQIETLRQVALKEYGKGNWVIIGGDWNMNPPGFDPDKITTGDETFTVET
                     EKIPNDFLPKGWKWIYDEKRPTNRSVVTSYTKGETPTTIIDFFLLSPNIQCLNVETIN
                     FAFSFSDHNPVFLEALLSNCH"
     gene            complement(11310..13463)
                     /gene="scpA"
                     /locus_tag="PK345_03745"
     CDS             complement(11310..13463)
                     /gene="scpA"
                     /locus_tag="PK345_03745"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004494 - methylmalonyl-CoA mutase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HPX34167.1"
                     /translation="MKPNFKNINIKHSVKSQLSSADWDKENHIEKNWLTPEQIKLKPV
                     YTEADLTKMEHLQYAAGIPPFLRGPYSTMYVLRPWTVRQYAGFSTAEESNAFYRRNLA
                     SGQMGLSIAFDLATHRGYDSDHERVVGDVGKAGVAVDSILDMKILFDQIPLDKMSVSM
                     TMNGAVLPIMAFYIVAGLEQGVRLEQLTGTIQNDILKEFMVRNTYIYPPLPSMRIIAD
                     IFKYTSKNMPKFNSISISGYHMQEAGATADIELAYTLADGLEYLRTGIQSGLDIDAFA
                     PRLSFFWGIGMNHFMEIAKMRAARMLWAKIVKQFNPKNPKSMALRTHSQTSGWSLTEQ
                     DPFNNVARTCIEAMGAVLGHTQSLHTNALDEAIALPTDFSARIARNTQIYIQEETNVC
                     RVVDPWAGSYYVDTLTHEIAHRAWKLIEEIEELGGMAKAIETGVPKMRIEEAAARKQA
                     RIDSFKDTIVGVNKYRLEKEDPIETLEVDNSAVRNAQLKRLEQLRAERNQEEMDAALS
                     KITKACETGEGNLLELAIEAAKQRATLGEISMACEKVFGRYKAVIRSISGIYSSESKG
                     DDKFKKACSLADEFAEIEGRRPRIMVAKMGQDGHDRGAKVVSTGYADIGFDVDIGPLF
                     QTPAEAAKQAVENDVHILGISSLAAGHKTLVPQVINELKKAGREDIMVIVGGVIPPQD
                     YQYLYDAGVVAVFGPGTVISEAGIKILEILIESRK"
     gene            complement(13497..>14534)
                     /locus_tag="PK345_03750"
     CDS             complement(13497..>14534)
                     /locus_tag="PK345_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPX34168.1"
                     /translation="LWAKIVNAYGPSDAELTRTYIHTITSDWNKTIYDPYVNMLRTTT
                     EAMSAIIGGTNSLFIKPFDSTFVEQNDFSERIARNQQLLLKEESYLDKIIDPAAGSYY
                     IETLTDSIVENAWNLFLEIMERGGFVASLKDGFIQERISQTAQERNLAIANRKEIFVG
                     TNQYPNFSEHLDPEIITEILKNKEEDSGKKTIEPLKIYRGSEEFEKLRAATDRWALSH
                     KRPTAFMLTYGNLAMRRARSQFSANFFGCAGYKIIDNNGFDTIQQGVDAALKSDAEII
                     VICSADEDYPVIAPEILDKTKHKAIVVVAGYPKDSIEELKSKGLKHFIHIKSNVLDTL
                     LEYNKMLGINS"
     assembly_gap    14535..14544
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<14545..15412)
                     /locus_tag="PK345_03755"
     CDS             complement(<14545..15412)
                     /locus_tag="PK345_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005467475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HPX34169.1"
                     /translation="MIDINASEEQKKLFDEFPSISTQEWEQMIYKDLKGADYVRRLIW
                     KTIEGFDVRPYYRAEDLEKINYLNIFPGDFPFVRSGRKKENNWLIRQDISVKNIKDAN
                     KKALDILMKGVDSVGWMLEQEKEPSIEEIEKLMENIYAQSVEVNFICGKHAPKVLDIY
                     IELVKKYNRDLQKINGSVNFDPISKLLRTGAFYQSENDDFASCSDIIKASHYLPHFRV
                     LSVDAVLFKNAGSSTVEELAFGLAYGNEYLQRITDLGVSIDNVAPKIKFNFGVGSNYF
                     MEIAKIRAARLLW"
     gene            15910..17823
                     /gene="dnaK"
                     /locus_tag="PK345_03760"
     CDS             15910..17823
                     /gene="dnaK"
                     /locus_tag="PK345_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008149022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HPX34170.1"
                     /translation="MGKIIGIDLGTSNSCVSVMEGNEPVIIPNSEGRRTTPSIVAFTE
                     NGERKVGDPAKRQAITNPKNTVYSIKRFMGETFDRVQKETGRVTYDVVKGENNTPRVK
                     IMDRQYTPQEISAMVLQKMKKTAEDYLGTTVNEAVITVPAYFSDSQRQATKEAGEIAG
                     LKVRRIINEPTAASLAYGLDKKKEDLKVAVFDLGGGTFDISILELGDGVFEVKATNGD
                     THLGGDDFDHEIIDWLASEFKADFNIDLKKDPMAMQRLKEAAEKAKIELSSSSETEIN
                     LPYIMPVDGIPQHLVKKLTRAKFEQITEKLTKATIEPCRKALADAGIKASDIDEVILV
                     GGSTRIPAIQRVVKEFFGKEPSKGVNPDEVVAIGAAIQGGVLSGDVKDVLLLDVTPLT
                     LGIETLGGVMTKLIDANTTIPTRKSEVFSTASDNQPSVEIHVLQGERSMARDNKTIGR
                     FHLDGIPPAMRGIPKIEVTFDIDANGILNVSAKDQGTGKSQSIRIEASSGLTDEEIKR
                     MKAEAQANAEADKKAKERVDKLNHADSLIFQTEKQLKEYGDKIPADKKKVIEDAITEL
                     KDAHKREDLDAIDRASKALNDAFQAASQDIYNATQQQGGEQAGQQSEGAAGGDHSGST
                     DQDVTDVDFEEVK"
     gene            18089..>20631
                     /locus_tag="PK345_03765"
     CDS             18089..>20631
                     /locus_tag="PK345_03765"
                     /inference="COORDINATES: protein motif:HMM:NF040146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitobiase/beta-hexosaminidase C-terminal
                     domain-containing protein"
                     /protein_id="HPX34171.1"
                     /translation="MKNIYNYLKSALLITALFFFSFMLKAQYFVDFEGEGEVKTAYAS
                     GTVTLSGLDWNMTEILIGTLDADWKNGERSARLRGYGTSVMTMLQDKENGIGNISFYY
                     RRYGTDQQVDWKVEYSTNNGSTWTQVGSAFTAPASDVVQHFDEEVNIDGPVRIRIKRA
                     TETGTSNKRLNIDDILVTDFTGGNPSVAKPVFTPPAGQYFGPVEVIITCDTPGADIYY
                     TTDGSDPTQSSNYYTAPVNISSTTTLKARAYAENLDPSSITQGTYNIAEVIEVANLGE
                     LRDLYSGGTEYFKVTSEVIVTFTQPFRNQKYIEDATAAILIDDLSGTIVTEYNIGDGI
                     TGIVGSITAFGNMLQFNPAFDPGAPTSTGNAILPQVITIEEMLANFEEYESELVKING
                     VTFSDAGEEFANGVVYPISDNSKATANFRTTFYDVDYIGTTIPSGVGNVVGILNSRNE
                     GDFITSRSLSDLEWYFGEPSNYPTMFEATSSGLSITLTWEDATGEILPSGYLILASDE
                     DDFTAPVDGVSVINDPVLADGEAAMNIAYGVEKYTFTNLEENVTYYFRIYPYTGSGST
                     IDYKTDGAPPAAEATVIFTFYPEPSNYPTEFAATAGSSSIKLTWNDATGDVLPTAYLI
                     LASVQNMFQYPYDGIPIEDDIDLSDGVGAKNVEQGVQQFTFTGLETETTYYFIIFSYT
                     NEAQYIDYKTDGEPPLTSATTQAPQIIDLLVTTFDESWESWTPVSVSGTQVWSRNNTY
                     GVGGTPCARISGYESSQFYVNEDWLISPTIDMNPVINPKIKFFSAFGYSGPALKLMIS
                     TDYNNQSNPNDAEWTDLTGEVIWPSGEPYFEWTGSGEIDLSSFEKEIIRV"
BASE COUNT         6335 a         3766 c         4046 g         6474 t
ORIGIN      
        1 acatagggta gcattgtggg aaaaacatgg ttgtatagca ataggaaagt ctcctgaaaa
       61 agcattcgat cttattgatg tattggaaaa atcagcattt atttttttca tttacaaaaa
      121 tgctggctat aatgtcgaag gaattaatga tattaaacaa aaaaaaatgc gaaggcattt
      181 taaaatttga ttactgtttt atttgtaata atatttaaat acattttttt gtgagtaaaa
      241 tttaaaatat ctttttaatg aaaaattata cgcttatagt agtttctgat ccatcaagtg
      301 aaagcgagtt tttagatttc ccatcaaggt tgtataaaag tgatccaaac tatataagac
      361 ctcttgatga agaggtaaaa gcaatttttg atccggcaaa aaacaagtta tttagaaatg
      421 gagatgcaaa gcgttggctc ttgaaagatg acaataacaa aactgttggc cggatagctt
      481 ccttttataa tgaagctctt tcaagagcta atgatcagcc tactggtgga attggttttt
      541 ttgattcaat aaacgatgtt gaagcgtcta agatattatt tgatgcttct cgtgagtggt
      601 tggtgggaaa aggtatggag gctatggatg ggccaataaa ttttggtgaa cgcgacagct
      661 tttggggctg tttggttgat ggtttttatc ctccaatttt caatatgcca tataattttc
      721 cctattataa agatttgttc gaaagttatg gctttcaaaa ctattttaat cagtacactt
      781 atcatcgttt gcttgtgcca ggcaagttaa ccacagtggt agaagagaaa gctatgaaaa
      841 ttctgaataa taaagattat cattttaaaa ctatagacac aaaaaatact gataaattta
      901 ttgaagattt catgattata tataacaaag catgggcgcg gtttccggga ataaagaaac
      961 tttcaaaagc tcatgctgcc gcacttttta aatcactgaa accaattatg gatccccgat
     1021 taatactata cggttattat aaaaatgaac ctgttgcttt ttttattatg atgcctgata
     1081 tcaatcagat tatcagggag tttgatggta aacttaatat gatcaataag ttgaaattga
     1141 tttttaagat taaatatact tcggctaccg ataaagttat cggaaggatt tttggtgttg
     1201 ttcccgaaca ccaaggtcgt ggggtcgaag ctggacttat taaaacgttt gaaaaaattg
     1261 ttataaagcc aaagtttcat tataaagatt tggaaatgaa ttggataggc gattttaatc
     1321 caattatgat gaaggtctgc gaacagattg gcgctaccat tcttaaaact catataactt
     1381 accgctattt gtttgacaga tctttgccat ttaagcgtgc aaaacgggtg aatgtgtaat
     1441 atggaatttc tgcttgctgt tgacctggga ataaatactg gattggcgct ttttggttct
     1501 gacgggaggc tttattggtt tcgttcaaaa aattttggtt ctgcttcaag gcttcgacgc
     1561 gcgatacctt ggctgttaaa tcaggaagaa gatataacta cagtaataat tgaaggcggc
     1621 ggccctttgt tgaaaatttg ggatgcagag tttaataaaa gaaatttgac ggttttaaga
     1681 ataatggctg accagtggag acaggaaatc ttttatcaac gcgagtacag aaaccgaaaa
     1741 gatgctaaag aaaatgcaaa atattatgct acgaaagtta ttgaaaagct atcggaaagc
     1801 ggattgacct ctttaaatga aaacactgct gaggctattc ttattggttt atacggaatg
     1861 aaaatgttga attggattga ttccataaat aaaacattaa ggtaaaaaaa acagtttttg
     1921 ttcgaattat ttcaagatca cgtttttagc caaatcattt aaggctataa agcattttgc
     1981 agttagtctt gcatcttcca aagcattgtg cgtatcgcca tcacgcgaaa aaccgaaata
     2041 agacgctacg ttttctaggc tgagcgatcg gggaatcttt ttaccattat tttcgagaat
     2101 cataaatgcg tattgtgaaa cactatgtag ctcaagcata cgaaaggaaa atggccatgc
     2161 taatttttct tttttataag caaaacttaa aaagttgata tcattgatta tactttgacc
     2221 acagataaca actcctttta gtgagtcttg ttcgcgataa aaaaggcatt tgcggatcca
     2281 tttttcaaaa tcggcaagta cttcataaat cattggcgca tcttccaact catcaattga
     2341 aataccatgt atctcctcag agtgacttga gaaagcatct tcattttcag ggtaaacgtt
     2401 gctgatgaac tctcccataa gtttccattt gtcatcatag atttcggcgc caatctgtat
     2461 tatctcgttg taatccggct ctgggccagt catttcaaga tcaaggacta aatagggcat
     2521 gattaaattt ttttaatgat aagcaaagat aaatggattt gattttagaa cgacagatac
     2581 atactttttc acaaatgaag agacgcgaat caataaatgg gacaagtatg tttacttagg
     2641 ttcgtttttc tttgaaatat ctttttaata attccccgca ttcattttgc aaaatccctg
     2701 taatcaattc tgttttagga tgtaaaatat ttttatttat tagagtaaat ccttttttgt
     2761 gatcgaaagc gccaaaaaca attttactta tttgcgtcca ataagctgct ccggcgcaca
     2821 ttacacatgg ttcaagtgta acgtaaagag tacattcgtc aagatattta ttgccggtat
     2881 aattggatgc ggcagtaaaa gcttgcattt ctgcatgagc tgtaacgtcg gtaagctgtt
     2941 cagttaggtt atgtgcacgg gcaataatac ggttgttgca aacaattaca gcccctaccg
     3001 gtacttcatc ttttgtaaag gcttcatatg cctcctttaa agcctctttc atataatatt
     3061 cgtgagtatt aaaattaaac atcagataaa acaattggag tgaacaatta aaaataaaac
     3121 ttggccaaat tagtaattga tttttgcatg caaacaataa taacgaattt ttgtttctat
     3181 tcacaactaa atcattaaaa cataatatta tgatatcgaa gaagatagaa tcagcgatca
     3241 atgatcaaat tttacttgaa gaattatctt cgagagtata tcttgcaatg gcctcttggt
     3301 gtgaggttca aggtttgcag ggtgttgcta attttttata taaacatagt gatgaagagc
     3361 ggatgcatca actgaaactt attcgttatc ttaacgatag ggaaggatat gctgagttgc
     3421 aagcgttgcc aaaacccgag ataaagtgta aagatattga agagctgttt acacaagttt
     3481 taaaacacga acagcttgta agtaagaaga ttaatgactt agttgatatg agcctcgaag
     3541 aaaaggatca tgccactttc aacttcttgc aatggtttgt ctctgaacag gttgaagaag
     3601 agagtttagt aaataacata ttggctaaaa tcaagttggc cggcgattca aaaatgggca
     3661 aattccatat tgacaatttc ctcgcatcac ttgatgatta gaaaaaagta aaattgttaa
     3721 agtattgtca attggatgat ctgggataat accgaaagga tgattgtttt ttcgatcggt
     3781 attatcctct tatctcttgt tttattcttt gtacatgacc tcgtccaagg gcttttactt
     3841 cgcagcctaa ttcaaaatat cgtttgattt tttcatccga taacataccg aaagcatcaa
     3901 ttactgctat cggcgctcca gcccaagata caatatcatc aggatttagg tcaaggtatt
     3961 ccgaatgagg aactgcaaaa attaaagctt ccaccccatt tactgatttt ttaagatctt
     4021 ttccaactct aatatctttt aaatgttctt gattgcggaa aaaccgtttc catgatgatt
     4081 ttggtgatgg ataagtatct tggttttcaa gttcatacca atggtcaacg taaggatcgt
     4141 gtacacgaat ctcggcgccc atttcggtaa gttttcttac taccatttcg ctgccgctat
     4201 atcgagtatc gcccacatcc tgacgataac ttgctccaca gatcaacacc tgcgatcctg
     4261 caatatatct tgccatattt ctaagggcat cacgaacaag ttgcgctaca tgcaacccac
     4321 gagtatcgtt gatatctatg gcagtaggtg taattttgaa aagtgattca ccatcttcaa
     4381 agccaagaat atgacgataa gaccaatagc ctaatcctcc atcttttggc aaacagtaac
     4441 caccaattcc aggacctggg aaaatcatat tgctatgagt aggtctcatt ttaatagcat
     4501 taattacttt aatcagatca acgccattac gctcggcaaa gatgctccat tcgtgcagat
     4561 aagcgagaat agttgcgcgg tatgagtttt cgattatttt ggtggtttct gattctatcg
     4621 gacgatccat aacggtaagc gggaaatctt tggtgttgat aacttcatgc aaaaattttt
     4681 ctactctttt gcgcgcttcg atatcacagc cactacacac acgccaaaaa tcacggatgc
     4741 tcgagacgta ttccttgcct ggcattacac gctcaaaact gtgagataac aaaggtgttt
     4801 cttttaaacc tcttttagca aatgcctttt taagaatagg ccatgccaca aattctgttg
     4861 tgccgggggc tacagttgtt tcgattaata caagacaatg tggcattatc gtttcaccta
     4921 ttgtacgcat agttgcttca agggcggcca tgtcggcttc accattacgc atgttgccaa
     4981 gttcacgttt ttcgtaatcg cattgtacat caaccactac cacatcagcc aattttaata
     5041 cttcgctatt ataggtagct attagtgttt ttttctcttt gacacaacgg gaaattaaaa
     5101 tatccacttc tggatcttct gcttttaccg gtgaaatacc ttgattgagt atcggtattt
     5161 tccaataact tctaacactt ggccgctgac aacctattac aaattttgag tcgttgccat
     5221 tttcatcttt ggtatcggca atgatagctg ccataacagc cccaacaaaa ccaaccccca
     5281 ttacaactac tatttcttta ccttcgctac gagctttatc agcaagtact tttatccgat
     5341 taaattcggc aggatattca tttttttcgg gtagtgggaa ttgttgacca tgaggactga
     5401 ttgagaaata attttccatg tatctgatta tttgaaagtt aaattattat attaaattaa
     5461 taaatgttat cgtaatagag cattagtttg tttttgaatc aatgcaaaaa tttttgccat
     5521 agttttttca atagtcttgt cggtaagtgt tttatgatca tcctgtaaaa aaaacccaat
     5581 ggcatatgat tttttcccct ttactatttt ctcaccctca taaacatcaa aaagaattac
     5641 ctctttaagc aggtcttttt cagcttcgaa agccagagtt tttaatgttt caaattcaat
     5701 attttgatca accactaaag caagatctcg cctgacctcc ggaaatttag gaattggctt
     5761 gtatgttatt ttatgatttc tcatttcgtg aatgatatta tcccaatgca agtcggccat
     5821 tatcaccgat tgctttattt caaaataatt taaaatacgt ctgttgacaa ctccgaactc
     5881 gattgttgat ttatcattca aaaacattct tattccgctt tcaaacagtt cgtttgaaat
     5941 attttcgttt ttaaatcttt tttcattaag attcagcctc tttaagatat taaaaaccgt
     6001 gttttttaga aagaaaaaat cggcttcatt ctgttgttga taccagcttt cgttatttgt
     6061 tttaccggtt ataaaaatgg tgagatgagt gttttcttta tattttttaa gagggtgcgc
     6121 tcccggttta gtttttatga gtgaataaac atttccgaat tcgaatagtt ttaaatcgag
     6181 atttttgtgt ttcaaattat ggcttattac ttcaaatccg gatgtaacaa ggctttgcct
     6241 cataacgttg aggtcggagc taagcgggtt ttgaattttt acgttatttt ctgtttcgta
     6301 aatcagtaat ttatcagcat attcgctttt agtaagcgag ttacacatta tctcataata
     6361 accatttccc gaaaggaaat ccgaaataat gttttgcaaa tattcgcgat caggtttttt
     6421 agtataagaa atagatgaac gtatttgaga cgggatggca atgttgttgt aaccgtatat
     6481 tctcagaatg tcttcaataa tatctgcttc tctatctaca tcggctttaa atgtaggtac
     6541 ttcaaccata agtgaattat ctgtttcatc aattatttcc atttcgagcc aaattaaaat
     6601 atctttaatt gtctctcgtg gtataaactg gccaattaaa caatcaacgt tttcatattt
     6661 tagttcaact attttctttt caatagggtt tggataaacg tcaacaatat cggaactgat
     6721 tgttccgccg gctattcgtt ttaccattaa tgcagcaagt tttaaagcat aaattgtggc
     6781 attaggatcg gtgccacgct caaaacgaaa ggatgaatcg gtatttaatc cgtgatatcg
     6841 tgcagtttta cggatagttt ttgaatcaaa atgggcgctt tcaataaaaa tattcttcgt
     6901 tgattccgac acacctgcat ataaaccacc aaaaacacca gccatacaca ttcccttttc
     6961 ttcatcacaa atcataaggt cttctcccga aagttcccgt tttacttcat ccagggtggt
     7021 aaactttgtt cctttaggct ctttttttac tatgattttt tttccggtta tcttatcagc
     7081 atcaaaaaaa tggtttggat gacctgtttc gtgaaggata aaattactaa tgtcaacaag
     7141 attgttaatt ggccgcagtc caatagcttt aaggcgattt tttaaccatt cgggtgattc
     7201 ttttacctca acacccgtaa tggtaacacc tgaatatctt ggacacgctt tggcatcttc
     7261 gatgattact gaaatcggat aatcgttgtt atcgggtctg aaggcgctat cgttgggttt
     7321 gataagtttt atcaggtcac atttcttttg aaggttgagc gctgctgcca aatcacgagc
     7381 tacaccgata tgggacatgg catccgacct gttgggtgta agccctattt caaaaacgta
     7441 atcctcttca accgaaaaat attcattagc aggtaaacca acttcggcat catcgggtaa
     7501 aacaactatt ccctcatggg agttgccaag tccaagttca tcttcggcac agatcatacc
     7561 ttctgaaact tcgccgcgaa tagttgtttt ttttatttca aataacttat ctttcatgtg
     7621 aatcattgtg ccaacagtag ccactaacac tttttgtcct gcggcaacat tgggagctcc
     7681 gcaaacaatt ggcaaaatat tgccctgtcc aacctctact gtagttatac tcaacttgtc
     7741 ggcgcctgga tgtttttcac aggtttttac ttcgccaatc actacccctt tcaggcctcc
     7801 ttttatgctt tcgaattttt cgatactctc tacttcaaga cctgtgttag ttaaaattgg
     7861 agcgatttct tcaggggtta aatcaatgtt tatatattct ttaagccagt tgtaggaaat
     7921 tttcatgaaa aatgttagat gaatttagcc cacaaaaata aataatctat gaatttcatt
     7981 aaaacaggca gtttttaata gttctgatga aaggatgaca ataagaaaag aaaaaaaaat
     8041 ctaaattgag cctttgcaat tttgggctat ttgttggctt ttttgtatct ttgcagcgct
     8101 ttaatgattt tattatcaat tgtttgatta tgaccgcaga agagaaaaac ttaatagcaa
     8161 ctaaagaata ttcagctgaa aacatacaag tgcttgaagg gcttgaggct gttaggaaaa
     8221 gaccatcaat gtatattgga gataccggag cacgaggcct tcatcatctt gtttatgaag
     8281 tggtagataa ctctattgat gaggctttag ctggatattg tgacaagatt gaagttgtta
     8341 ttcatgagga tgaatcaata accgtaaaag ataatgggcg tggtattccg gttggcatgc
     8401 atgaaaaaga gaaccgctcg gcgcttgaag tagtacttac tgttttacat gccggcggta
     8461 agtttgacaa aggatcttat aaagtttcgg gagggttgca tggcgttggt gtttcggttg
     8521 tcaatgcttt atctaaagta ttgaaagcgt ctatctatcg cgaagggaag ttattttatc
     8581 aggaatacaa ttatggaaaa ccattagaac cggtaaaagc aattgccgaa accaaagata
     8641 caggaacgga aattaccttt agccccgacc caacaatttt tccggaaatc aagttcgatt
     8701 ataatatcct tgctacacgc ttacgtgagc tttcttttct taataaaacc attagcctct
     8761 ctttaacgga tgaacgtttg acagatgaag atggaaattt tgtaagcgaa gaattttact
     8821 ctgaaaatgg attgctagat tttatcaatt atctggatga aacccgcgag aagcttattc
     8881 ctgaggcgat ttatatcgaa ggtgaaaaaa acaatacacc tattgaaatt gcaatgcaat
     8941 acaacacctc tttttctgaa aatcttcact cttacgtcaa caatatcaat actcatgaag
     9001 ggggaactca tttgacagga ttcagaagag ggcttacccg tacacttaaa gcttatgccg
     9061 agaagtcggg tttattggca aagctgaaat ttgatatcaa tggagatgat tttcgcgaag
     9121 gtctgacagc aattatttct gtaaaagtgg cagaacccca attcgagggt caaacaaaaa
     9181 ccaaattagg taattccgac gtgatgggtt ctgttgatca gatggttagc gaactgttga
     9241 caaattatct tgaagaaaat ccgcgcgaag ccaaaaccat tgtcaacaaa gttattttag
     9301 ctgctacagc acgtcatgca gcaagaaaag cgcgtgaatt agttcaacgt aaaaatgtcc
     9361 ttacaggatc gggacttccc ggaaaacttg ctgattgttc cgaacgtgat cctgagcttt
     9421 gtgagattta tttagttgag ggcgattctg caggtggaac agcaaaagct ggacgcgacc
     9481 gccgttatca ggcaatttta ccgttgcgag gcaaaatctt aaacgttgaa aaagccatgc
     9541 agcacaagat cttcgaaagc gaagagataa aaaatatatt caccgcgtta ggggtaacaa
     9601 tcggaacaga tgaagacagt aaagcgttga acattgagaa actacgctat aataaagtca
     9661 taatcatgac agatgctgat gtggatggaa gccatattgc cacgctcata cttacttttt
     9721 tctttagata tatgaaggag cttattgaga tgggtaatat ttatattgcc acaccgccac
     9781 tttatctgat aaaaaaagga aaagacgagc gctattgttg gacagaggaa gagcgaaata
     9841 aaattgtaac aaaatggtcg gtaaatggcg atgctaaagg aatacatatt caacggtata
     9901 aaggcttggg agaaatgaac gctgagcagt tgtgggatac aactatgaat cctcaaaata
     9961 gaatactaag acaagttact attgaaaatg gaacggaggc cgatcgtatt ttttcaatgc
    10021 tgatgggcga cgaagtaccg ccacgccgtg agtttattga aaaaaatgcc agatacgcta
    10081 aaattgatat ctaaaatact gatttataga aaagttgaaa ttgatcatac gtagtattat
    10141 tgtatttacc ataatacttg ttttcatatt actcatcttt ctgcttttct ctactttaga
    10201 tgagtacaag ccatccaaca aagagatttt acatccagag acattttctt ttagcaaaac
    10261 cttaagcgat agcatcatta gcttgatgac gtggaatatt ggctatgctt cgatgggcag
    10321 cgagaccgat tttttttatg atggcggaaa aatggtcagg ccaaatagag aactccatga
    10381 aaaatatctt gatagcattg ttaatttttt tcaacccgaa aaggcaccaa attttatttt
    10441 attgcaagag gttgattatg aggcaaagag atcatatttt tcaaatgaaa aagagacctt
    10501 gcaacgaaga ctgatagatt attcttctgt ttttgctgta aattacaatg tcttttttgt
    10561 tcctgtgcca ctatttaatc ctatgggaaa agttattgcc ggcatgatct cgttttctga
    10621 cgtagagcct aaatcatctt ttagaatcgc tttcgcggga aattaccctt ggccggtaag
    10681 actttttaat ctgaagaggt gttttatatt gcaacgtttt aatactcaaa gtggtaaaga
    10741 gttgattctg ataaatattc ataattctgc ctttgataaa ggtgatttgc gtaaaattca
    10801 aatagaaacc cttcgtcaag tagcattaaa ggagtatggt aagggaaatt gggtaattat
    10861 tggaggtgat tggaatatga atccaccggg atttgatccg gataagatta ccaccggcga
    10921 tgagactttt accgttgaaa cagaaaaaat tccaaatgat tttttaccta aaggatggaa
    10981 atggatttat gatgaaaaga ggcctacaaa ccgttctgtg gttacaagct atactaaagg
    11041 agaaacccca acaactatta ttgatttctt tcttctttca cctaatattc aatgtcttaa
    11101 tgtggaaact ataaactttg cattctcttt ttccgaccac aatccagtat ttttagaagc
    11161 gctgctttcc aactgtcatt aatattttat ctcaattaat caagaggtgg ctatttcagg
    11221 ctaaaccatt tattttataa gtctaatcgt agtttggtgt ttgtttggaa caatttttaa
    11281 tgtgttgttc acaaccattt aatttgggtt tactttctcg attctatcaa aatctcaagg
    11341 attttaatgc ccgcttctga aattactgtt ccaggaccaa aaactgcgac aaccccggca
    11401 tcataaagat attgataatc ttgaggaggg attacgcctc caacgataac cataatatct
    11461 tcccgtccgg ccttttttaa ttcgttgatt acttgtggta ctaatgtttt atgacctgcg
    11521 gcaaggcttg atattccaag gatatgcaca tcattttcca cagcttgttt agcagcttca
    11581 gcaggtgttt ggaacaaagg ccctatatct acgtcaaagc caatatcggc atatcctgtt
    11641 gagactactt tggcccctcg atcgtggcca tcctgtccca tcttagcaac catgatcctt
    11701 ggacgccgcc cttcaatttc ggcaaattcg tcagctaagc tgcaagcctt tttaaactta
    11761 tcatcacctt tcgattctga agaatagata ccagatatcg aacgtatcac agctttatat
    11821 ctaccaaaaa ccttttcgca ggccattgaa atctctccga gtgtagctct ctgtttggca
    11881 gcttcaatag caagttccaa caagttgcct tcgccggttt cacaggcttt ggtaatttta
    11941 gatagcgccg catccatctc ttcctgattt cgttcagcac gcaattgttc aagccttttt
    12001 aattgagcat ttctaaccgc tgaattatct acttcaagag tttcaattgg atcctctttt
    12061 tcgagtcggt atttatttac acccacaatt gtatctttaa aagagtcaat tcgtgcttgt
    12121 ttgcgtgctg cagcctcttc aatacgcatt tttggaactc cggtttctat tgcctttgcc
    12181 ataccgccca actcttcgat ctcctctatc agtttccatg cacggtgtgc aatttcatga
    12241 gtcaatgtat cgacataata ggagcctgcc catggatcta ccactcgaca aacgttagtc
    12301 tcttcttgaa tgtaaatttg tgtgttacga gctatacgtg ccgaaaagtc agttggcagg
    12361 gcaatagctt catccaaggc atttgtatgt agcgattgtg tgtgtccaag tactgctccc
    12421 atagcctcga tacaggtacg ggctacattg ttaaaagggt cttgttcggt aaggctccat
    12481 cccgacgttt gggaatgagt acgtaatgcc atggattttg gattttttgg attaaattgt
    12541 ttaacaattt ttgcccaaag catacgcgct gccctcattt tggcaatctc cataaaatga
    12601 ttcattccaa taccccagaa aaaggagaga cgcggagcaa aagcatcaat atctaatcct
    12661 gactgtatgc cggtgcgaag atattcaagt ccatcagcta aagtataggc taattcgata
    12721 tcggcggtgg ctcctgcttc ttgcatgtga taaccgctga tgctgataga gttgaattta
    12781 ggcatattct tagaagtata tttgaagata tcggcaatga tacgcattga gggcaatggt
    12841 gggtaaatat aggtgttgcg caccataaat tccttgagga tatcattttg gatagttcct
    12901 gtaagctgtt caaggcgaac gccttgctcc aggcctgcta ctatgtaaaa ggccattatt
    12961 ggtaatacag ctccattcat cgtcattgag accgacattt tatccaaagg gatttgatcg
    13021 aaaaggattt tcatgtcaag tattgaatca acggcaacgc cagctttgcc aacatcgccc
    13081 acaacccttt cgtgatccga atcgtatccg cggtgggtgg ctaagtcgaa ggctatagag
    13141 aggcccatct gccctgaagc caagttacgg cgataaaaag cgttcgactc ttcagccgta
    13201 gaaaaaccag catactgccg aacagtccac ggacgtaaaa cgtacatggt agaatatggc
    13261 cctctaagaa atggaggaat acctgcagca tattgcaaat gctccatttt ggtgagatct
    13321 gcttcggtat agactggttt gagttttatc tgttcgggtg tcaaccaatt tttttctata
    13381 tgattttctt tatcccaatc ggcgctgcta agctgcgact ttacagagtg ttttatattg
    13441 atatttttga aatttggttt cattgtataa aatgttaaat ctttaataaa attatattat
    13501 gaattgatac ccagcatttt gttatattca agcaacgtat caaggacatt ggatttaata
    13561 tgaatgaaat gctttaatcc ttttgatttt aactcttcga tactatcttt aggatatcct
    13621 gcaaccacaa caattgcttt atgtttggtt ttatcgagga tttcgggagc aattacgggg
    13681 taatcttcat ctgcagaaca aatgacgatg atctcggcat cactttttaa tgctgcatca
    13741 actccttgtt gtattgtatc aaacccgttg ttatcaataa ttttataacc tgcacagcca
    13801 aagaaattag ctgaaaactg cgaacgtgcc cgccgcatgg caaggttgcc gtaggtgagc
    13861 ataaaagcag tcgggcgctt atgacttaaa gcccaacgat cggtggctgc cctcaatttt
    13921 tcaaactctt cagaaccacg gtaaattttc aatggctcaa tagtcttttt gccactatct
    13981 tcctctttgt tttttaatat ttctgtaata atttccggat caagatgctc ggaaaaattt
    14041 ggatattggt tggttccgac gaatatctcc tttcggttag caatagctaa attacgttct
    14101 tgtgcagttt gggaaattcg ttcctgaata aatccatctt ttaatgatgc tacaaatcct
    14161 cccctctcca taatttctaa aaagagattc catgcatttt caacaatcga gtctgtgagt
    14221 gtttcgatat aataagaacc ggctgcggga tcaataattt tatcgagata cgactcctct
    14281 tttaaaagca attgttgatt tcgggcaata cgttccgaaa agtcgttttg ctctacgaat
    14341 gttgaatcaa aaggttttat aaaaagcgag tttgttcctc cgataatagc cgacatagct
    14401 tcggttgtcg ttcttagcat attaacataa ggatcgtata tggttttatt ccaatcgctt
    14461 gtaatagtat ggatataggt acgagtaagc tcagcatcgg aagggccata tgcatttact
    14521 attttagccc aaagnnnnnn nnnncccaaa gcagcctggc agctctgatc tttgcaattt
    14581 ccataaaata attggagcct acgccaaagt taaatttaat ctttggcgct acattatcaa
    14641 ttgaaacacc gagatctgtt attctttgta agtattcatt accataggca agaccgaaag
    14701 ccaactcttc gactgtggat gaaccggcat ttttgaataa aacagcatct actgacagta
    14761 ccctgaaatg aggtagataa tgcgaagcct taatgatatc tgaacatgaa gcaaagtcat
    14821 cattttccga ttgataaaaa gcgccggtac gaagcaattt actgatcgga tcaaaattga
    14881 ctgagccatt tattttttga agatcacgat tatacttctt tactaattcg atataaatat
    14941 caagaacctt aggagcgtgt ttaccacaaa taaaatttac ttcaacactc tgggcataaa
    15001 tgttttccat taatttttct atctcctcaa tgctcggctc tttttcttgt tcaagcatcc
    15061 agccaacaga atctacccct ttcatcagga tgtcgagagc ttttttgttg gcatctttta
    15121 tgttttttac tgagatatcc tgccgaataa gccagttgtt ttctttttta cgtccacttc
    15181 ttacaaaagg aaaatcccca gggaaaatgt tcaaataatt gatcttttca aggtcttctg
    15241 cacgataata tggcctgaca tcaaaacctt caatggtttt ccaaattagt cttcgcacat
    15301 aatcagctcc ttttaaatcc ttataaatca tttgttccca ttcttgggta gaaatagaag
    15361 gaaattcatc aaacagtttt ttctgttctt ctgatgcatt aatatcaatc atttttatct
    15421 ggttaagtta tttattatta atgaattatt caatatgaca agtttttata ataaggtgtg
    15481 caaagttacg acaaatcatg gatgaaaagc taattatact caagttggta aagtaaagta
    15541 agttaaaacc agattattca atagaaaatc cttgttggct catctgatta tttatgaggt
    15601 caattccgat taagtgtctt tttcgtacaa tttttttgta gtaattacta acttttaata
    15661 tggatttata cttgtttctg tcgttttgac aggtgaaaat gtcagggttt gaaatacaga
    15721 ttttttgatt atgattaaag ttaacgtgtt tataatcaat gatataattt taaaaaaagg
    15781 aaatttaaaa aattatcatt aaaagagctt ggtggttaaa cagcttccta acagtctgtg
    15841 gcataatgat tgacggagca gtgcgcaaat caaatagaaa ttaaaaatta taattaaagt
    15901 aataaactaa tgggaaaaat aatcggaatt gatttaggta catctaattc gtgtgtttcg
    15961 gtaatggaag gtaacgagcc tgttattatc cccaattcgg aaggacgtag aactacccct
    16021 tccattgtgg catttaccga aaatggcgaa cgaaaagttg gcgatccggc caagcgtcag
    16081 gcaatcacaa atccaaaaaa cactgtttat tccatcaagc gtttcatggg cgaaacattt
    16141 gatagggtgc agaaagagac cggtcgtgta acttatgatg ttgtaaaggg tgaaaataat
    16201 accccaaggg taaagataat ggatagacaa tatacccctc aagagatatc ggcaatggtt
    16261 cttcagaaga tgaagaaaac cgcagaggat tatcttggta ctactgttaa tgaagctgtt
    16321 attactgttc ctgcttattt cagcgactcg cagcgtcagg ctaccaaaga ggcaggcgag
    16381 atcgcaggtc ttaaggtgcg gaggatcata aatgagccta cggcagcttc gttagcctat
    16441 ggtttggata agaaaaaaga ggacctcaaa gtagcagtat tcgatctcgg tggcggcaca
    16501 tttgatattt ctattcttga gttgggcgat ggcgtctttg aagtgaaagc aacaaatggc
    16561 gacacacacc ttggcggaga tgattttgat catgagataa ttgattggtt agcctctgag
    16621 tttaaggccg attttaatat tgaccttaaa aaagacccaa tggctatgca gcgcttaaaa
    16681 gaagctgctg aaaaagcaaa aatagagttg tcaagctcaa gcgaaacgga gattaatctg
    16741 ccttatatta tgccggttga cggtattccg cagcatcttg tgaaaaaact cacacgtgca
    16801 aagtttgagc aaatcactga gaagcttacc aaagcaacca tagagccttg tcgtaaggca
    16861 ttggctgatg caggaataaa ggcctccgac attgatgagg taatcttggt tggcggatca
    16921 acacgaatac ctgctatcca gcgggttgta aaggaatttt ttggtaaaga accctccaaa
    16981 ggagtaaatc cggacgaagt agtagctatt ggagcagcta ttcagggagg ggtactttcc
    17041 ggtgatgtaa aagatgtgct gttgctcgac gtaaccccgt tgacgttagg tattgaaacc
    17101 ttaggcggtg tgatgacaaa actaattgat gccaacacaa ctatccctac ccgaaaatca
    17161 gaagtcttct ccacagcttc tgacaatcag ccatcggttg aaatacatgt attgcaagga
    17221 gagaggtcaa tggcaagaga taacaaaaca attggaaggt ttcatctcga cgggatacca
    17281 ccggcaatga gaggaatacc taaaattgaa gttacttttg atattgatgc taacggaata
    17341 cttaatgtgt cggctaaaga tcagggtaca ggaaaatcgc aaagtatcag aatagaagcc
    17401 tcctcaggcc tgacggatga agagattaaa aggatgaaag ccgaagctca ggccaacgcc
    17461 gaagctgata agaaagcaaa agaacgtgtg gataaattga atcatgccga ctccttaatt
    17521 tttcaaaccg aaaaacagtt gaaagagtat ggagataaaa tccccgctga caagaaaaag
    17581 gttattgaag atgctattac cgagcttaaa gatgcccata aacgtgagga cctggatgca
    17641 attgacagag caagcaaagc attgaacgac gcttttcagg ctgcaagcca ggatatatat
    17701 aatgccacgc agcagcaagg cggagagcag gcaggtcaac aaagtgaagg agcggcaggc
    17761 ggtgatcatt ccggaagcac agatcaagat gtaacagacg ttgactttga agaagtgaag
    17821 taaagacaat taaatttata ttaaaaaatc ccggtgatta atttcaccgg gattttttgt
    17881 tggttgttga tgttcaagaa aatatgattt tatttaagat attaagacaa aaacttttta
    17941 acaggttggc gctgatcgct cacaattgat tatttttagg ccttggtttt aagataaaaa
    18001 tcaactaaga atgtataaga tctacttaat tatcagtggt ttattttaaa tgcttcgata
    18061 ataactatta aacatatact tttaaattat gaaaaacatc tacaactatt taaagtccgc
    18121 tcttttaata acagcgttgt tttttttctc ctttatgctt aaggctcagt actttgttga
    18181 ctttgaaggt gagggcgaag ttaaaacagc atacgcttca ggcacggtta ccctcagcgg
    18241 tttggactgg aatatgaccg aaatacttat cggcacattg gatgctgact ggaaaaatgg
    18301 agaacgctcg gctcgacttc ggggatatgg cacctcagtg atgacgatgt tgcaggataa
    18361 agaaaatgga attggaaaca ttagctttta ttatcgtcgc tatggtactg atcaacaggt
    18421 tgattggaaa gtggaatata gtaccaataa cggatcaacc tggacacagg taggtagcgc
    18481 cttcactgct cctgcttcgg atgttgttca gcattttgat gaggaggtga acattgatgg
    18541 tccggttcgt atcagaataa aacgagcaac agaaacagga acatcaaata aacgccttaa
    18601 tattgatgac attcttgtta ccgattttac cggaggaaat ccctcggtag cgaagcctgt
    18661 ttttacaccg ccggcgggtc agtattttgg tccagttgag gttataataa catgcgatac
    18721 acctggagct gatatttatt ataccaccga tggcagcgac cctacgcaat catcaaacta
    18781 ctatacagct ccggttaata tctcttcaac cacaaccctt aaagcaagag cttatgccga
    18841 aaatcttgat ccaagtagca taacacaagg cacttataac attgcagagg ttatcgaagt
    18901 ggcaaatctc ggcgaactca gagaccttta ttcgggtgga accgaatatt ttaaagttac
    18961 aagcgaagtg atagttactt ttactcaacc cttccgcaat caaaaatata ttgaagatgc
    19021 aacagcagcc attttgatag atgacctttc aggaacgatc gttaccgaat acaatattgg
    19081 tgatggaata accggtatag ttggctccat aactgctttc ggcaatatgc ttcaatttaa
    19141 tccggcattt gatccggggg ctcccacctc aacaggaaat gctattttgc cacaggtaat
    19201 aaccattgag gagatgctcg caaattttga agagtacgaa tcggaattgg tgaagattaa
    19261 tggagtaact ttttcggatg ccggtgaaga atttgctaat ggcgtagtat atcccataag
    19321 tgataacagt aaggcaaccg ccaatttcag aacgacattt tatgatgtgg actatattgg
    19381 aaccactatt ccatcaggag taggaaatgt tgttggcata cttaactcga gaaacgaagg
    19441 tgattttatt accagtcgtt cgttatccga tctggaatgg tattttggcg agccttctaa
    19501 ttatccgaca atgtttgaag cgacatcatc cgggttatca attactttga cttgggaaga
    19561 tgctacagga gaaatattgc caagcggcta tttgatatta gccagtgatg aagatgactt
    19621 taccgcccct gttgacggcg tatccgttat caatgatcct gtgcttgctg atggcgaagc
    19681 agctatgaat atcgcttatg gagttgaaaa atataccttt accaatttgg aagaaaatgt
    19741 aacttactat tttagaattt atccctatac tggttccggt agtacgattg attataaaac
    19801 tgatggcgct ccaccggctg ccgaagccac tgtaattttt actttttatc ctgagccgtc
    19861 aaattatccg accgaatttg ctgctacagc cggctcctct tccattaaac ttacctggaa
    19921 tgatgccaca ggtgacgtat tgccaacagc ttaccttata ttggcaagtg ttcaaaatat
    19981 gtttcaatac ccctatgatg gcattccgat agaagatgac attgatttga gcgatggcgt
    20041 gggagcaaaa aatgttgaac agggcgttca acaatttacc tttaccggac tcgaaaccga
    20101 aaccacttac tatttcataa tattctctta tactaacgaa gcccaatata ttgattataa
    20161 aactgatggt gagccgcctt taacttcggc aacaacacaa gcgccgcaga taatagatct
    20221 cttggttacg acatttgacg aatcgtggga aagctggact cccgttagtg tgagcggcac
    20281 ccaggtatgg agcagaaaca atacttacgg tgttggaggc actccatgtg cacggattag
    20341 cggatatgag agttcacagt tttatgttaa tgaagattgg cttatttcgc cgactataga
    20401 tatgaaccct gtaataaatc ctaaaatcaa gtttttttcc gcatttggct atagtggccc
    20461 tgccttgaag ctaatgattt caactgatta taacaaccag tcaaacccaa atgatgctga
    20521 gtggactgat ttgacgggag aggtaatatg gccttcggga gagccttatt tcgaatggac
    20581 aggctcaggc gaaatagacc taagtagttt tgagaaggag atcattagag t
//
LOCUS       DAOOAI010000033        20364 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_3481_length_20364_cov_5.763258, whole genome shotgun sequence.
ACCESSION   DAOOAI010000033 DAOOAI010000000
VERSION     DAOOAI010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20364)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20364)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20364
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3481_length_20364_cov_5.763258"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /gene="rlmN"
                     /locus_tag="PK345_03770"
     CDS             complement(<1..661)
                     /gene="rlmN"
                     /locus_tag="PK345_03770"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007658917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0002935 - tRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0070040 - rRNA
                     (adenine-C2-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HPX34172.1"
                     /translation="MNIKPQLFGKTLNELREIVSQLELPVYTAVQIADWIYQKEVVSF
                     SEMTNISLNNRKKLEANFDLGLIAPSKVQISADGTKKYLFEVARFKYIEAAYIPEETR
                     NTLCVSSQVGCKMGCLFCMTGKQGFQSNLTAGQILNQIRSIPERKSLSNIVYMGMGEP
                     FDNMDEVMKSLEILTSDWGYGWSPKRITVSTIGIVPAIKRFISDSQCHLAISLHSPFE
                     EE"
     gene            complement(668..1339)
                     /locus_tag="PK345_03775"
     CDS             complement(668..1339)
                     /locus_tag="PK345_03775"
                     /inference="COORDINATES: protein motif:HMM:NF012402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPX34173.1"
                     /translation="MLDFTGSKKLDLHPIKVTQLIEIAPNVFLLSFNRSFSFVPGQVV
                     ALAMNPADQYRYYSIASGMLEDEVKILFDLKPSGILTPALAKLKPGDTLFCSLPTGNF
                     YGTAKPAVWIAAGTGIAPFVSMWRSGLKKDKILIHSGRFLHSFYFEDEFKPLGNNYIR
                     CCTQEHVNGIFFGKINKWISEQSILPDDRRYYLCGSPEMVIDTIDALIKKGISLENII
                     AEIYF"
     gene            complement(1497..1931)
                     /locus_tag="PK345_03780"
     CDS             complement(1497..1931)
                     /locus_tag="PK345_03780"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPX34174.1"
                     /translation="MSEPKKDKKFIKLPKYPGGKAAFQEFIRKNLKYPPEALKNKIEG
                     AVHIKYRVNGLGKVVEAKVIHGLGYGCDEEALRLIWLLKYEKAKNRGLRVTAAMKTRI
                     NFKLPQKVPVEIKYEVKKEIKPKTSPEKAKPEKVSYNYIIEF"
     gene            2194..2481
                     /locus_tag="PK345_03785"
     CDS             2194..2481
                     /locus_tag="PK345_03785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34175.1"
                     /translation="MIDFSVTNEHLGITDKYCGFVNRWLVPNHLNYDEGRMNGSMGKE
                     DGGHGQSLLDDALALEELGSNCTGIDICIDANTPAFTPLYVAVFDTLKNKN"
     gene            2485..2976
                     /locus_tag="PK345_03790"
     CDS             2485..2976
                     /locus_tag="PK345_03790"
                     /EC_number="1.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006801191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004146 - dihydrofolate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HPX34176.1"
                     /translation="MISIIVAIAENYAIGKDNRLLWHISEDLKRFKRLTTGNTVIMGR
                     KTWLSLPKRPLPNRLNVVLTKNPKKCSEGCVMVDSIEAALEICSQAKECFIIGGETVY
                     RQMMPLADRLYVTRINKSFEADTFFPEISPELWKLTYLSEWKEMDDKSFSYRFEIWDK
                     KED"
     gene            complement(3056..5014)
                     /gene="dnaG"
                     /locus_tag="PK345_03795"
     CDS             complement(3056..5014)
                     /gene="dnaG"
                     /locus_tag="PK345_03795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HPX34177.1"
                     /translation="MIPKETIQEILDTARIEDVVMEFVNLKKRGATYIGLCPFHNEKT
                     PSFTVTPRLGIYKCFGCGKGGDSVNFVMEHEHFTYPEAIKYLARKYNIEIKDEEQTAE
                     RIEEQQEKESLFMVNDFASKYFIHNLFNTDAGRSIGLSYFKERGFREDTIKKFQLGYS
                     IDEWENFTAHALQQGFKLEYLEKTGLTITKDKKSYDRFRGRVVFPIHNLSGRVLGFGG
                     RTLLSDKNIPKYVNSPESEIYNKSRILYGIHLAKSAIIATDNCYLVEGYTDVISLHQS
                     KVENVVASSGTSLTTDQIRLISRYTKNITILYDGDEAGIKASFRGIDMILGQGLNVKI
                     VFFPEGEDPDSFSQSHTTSEVQDFLEGQAVNFIIFKTNLLLKETAGDPVKKSALIKEI
                     VGSIALIPDQITRSVYVKECSALMDVGEQTLMNVLNQIRRKEFDKKLKERQKDGESEL
                     AHLPQYIIPDVQHPGEEAVSTENHEKEIIRLLMQYGNRNIVFETMTEDEEVQTFEVSI
                     ALFITKDLENDEIRMKNELYQRVLHSASEQIESGTIPAESFYLHHSDKQISTMATNLL
                     AQKYELGNWEKVKIKVNIEEEILKLSVTHAILSFKLRVLESKYEDNLKQMQNETIQEN
                     LDILMDAQLKLRKKISMVSNELGRIVLR"
     gene            complement(5540..6022)
                     /locus_tag="PK345_03800"
     CDS             complement(5540..6022)
                     /locus_tag="PK345_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPX34178.1"
                     /translation="MSSIKIKLKEKDVQQIKEIASSFGNKPGELINVLHKTQGVFGYL
                     PAEVQEVVAREMNVSVAKVYGVVTFYSFFTMIPKGRYPISICTGTACYVRGAEKVLDE
                     FKKELGIKVGESTPDGKFSLTCLRCVGACGLSPVVTVGTKTFGRVSPDKVKDILKEFD
                     "
     gene            complement(6053..7816)
                     /locus_tag="PK345_03805"
     CDS             complement(6053..7816)
                     /locus_tag="PK345_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent [FeFe] hydrogenase, group A6"
                     /protein_id="HPX34179.1"
                     /translation="MIKLIIDKKTVEVKPGTTILQAADSIGIHIPRLCYMNLSDFNIE
                     NKPGGCRVCVVEVEGRRNLAPACATECAEGMVVNTHSIRVLNARKTVMQLILSDHPFD
                     CLVCAKSGNCELQELAQTMGIRDIPYQGEQSTYREDTSPSIIREVDKCIMCRRCEVMC
                     NEVQTVGVLSAINRGFTSVVAPAFEMNLDHSECTFCGQCVNVCPTGALTEVDHTNQLI
                     NDLADSSKTVIVQTAPAVRAALGEAFGMKPGTLVTGKLVSALRELGFDYVFDTDFAAD
                     LTIMEEGAEVLDRLTKFLNGDKNVKLPILTSCCPAWVKFFEHQFPQLKDIPSTARSPQ
                     QMFGSIAKSYFAEKIGVDRKNLVVVSIMPCVAKKYECTREEFSVDGNPDVDYSVSTRE
                     LANLIKRANIDFIALPDGDFDNPFGESTGASVIFGTTGGVIEAAVRTTYELYTKKHLD
                     KVDFKQLRGMEGIRSAKVDFNGLELNIGIAHGLGNARKLLEEVIEGKCAYHAIEIMAC
                     PGGCIGGGGQPYIHGDTSILKARQKAVYEEDVNKTLRKSHENPYIIRLYEEFLGKPLS
                     EKSHNLLHTHYFDRKKEIIIE"
     gene            complement(7842..9638)
                     /locus_tag="PK345_03810"
     CDS             complement(7842..9638)
                     /locus_tag="PK345_03810"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HPX34180.1"
                     /translation="MAKQIKMHLLVCCGTGCRASQSATLQKALERELKTNGLEDEAKV
                     VTTGCFGFCEKGPIVKVHPDNTFYVEVKPEDAEEIVKEHVLKGRKVERLLYTDPKVKE
                     HVPDSKHMGFYRKQIRIALRNCGLIDPENIDEYLARDGYQALGKVITEMTPESTIDVI
                     KKSELRGRGGAGFPTGLKWEITRKSKSDQKYVVCNADEGDPGAFMDRSILEGDPHVVL
                     EAMAICGYAIGADKGLIYIRAEYPLAIHRLKIAIEQAREYGLLGKNLFNSGFNFDIDL
                     RYGAGAFVCGEETALIHSMEGERGEPTFKPPFPSVEGYLGKPTNVNNVETFANVPIII
                     NKGADWFKTIGTEKSKGTKVFALAGKINNVGLIEIPMGTTLREVIFEIGGGIKDGKKF
                     KAVQTGGPSGGCLTEKHLDLPIEYETLVAAGSMMGSGGMIVMDEDDCMVSVAKFFLDF
                     TVEESCGKCSPCRIGNKRLYEMLDTITKGEGTMETLDKLKNLSKVIKDTSLCGLGQTA
                     PNPVLSTINNFWDEYVEHVVDKKCKASVCKSLMRYVVIPENCVGCTVCARNCPVNCIK
                     GERKQVHEIDQTACIKCGACYEKCKFNAIEIV"
     gene            complement(9656..10051)
                     /locus_tag="PK345_03815"
     CDS             complement(9656..10051)
                     /locus_tag="PK345_03815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPX34181.1"
                     /translation="MTKIKSLADLRKKKEEFEQKLKLRETSINPENYVQIKVGMATSG
                     IASGAKEVMDYLIEELGKRGIDAVVTQTGDMGYCFAEPTIEVKRPNEDPIVFGYVDTK
                     KADEIIEKYIKNGELVEGIIPVNYETIDE"
     assembly_gap    10068..10077
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(10438..11376)
                     /locus_tag="PK345_03820"
     CDS             complement(10438..11376)
                     /locus_tag="PK345_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HPX34182.1"
                     /translation="MKTRITELFDIRYPIIQAGMIWCSGWRLAAAVSEAGGLGLIGAG
                     SMYPDVLKEHIQKTKAATKKPFGVNIPLLYPEIDKIVEIVLKEKIKIVFTSAGNPKTW
                     TPIFKEHGIKVVHVVASSKFAIKAQDAGVDAVVAEGFEAGGHNGIEETTTLVLIPIIR
                     KAVDLPLIAAGGIGTGRQMLATFALGAEGVQVGSRFVVSTESSAHPIFKQKIIEAKEG
                     DTQLTLKRLLPVRLIKNKFYNEINEMQASGATIEQLKAHLGKGRAKLGMFEGNTDEGE
                     LEIGQGSALINSIMPAGEIIREMITEFEKAKSELTE"
     gene            complement(11485..13650)
                     /locus_tag="PK345_03825"
     CDS             complement(11485..13650)
                     /locus_tag="PK345_03825"
                     /inference="COORDINATES: protein motif:HMM:NF021942.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S46 family peptidase"
                     /protein_id="HPX34183.1"
                     /translation="MKSIFNRLILLLLILFPFISFADEGMWIPMLLEKMNEKDMSRLG
                     MRITASDIYDINNSSMKDAIFLFGGGCTASAISAEGLLLTNHHCGYGAIQRLSTIEND
                     MLTNGFWAVGRQDELYCPGLTVTRLVKMEDVTAAALSGVTDDLTEEARAEIIEKNIKE
                     IKDEATKDTHYEAVVKPFFYGNQYYLFVNEIFKDIRLVGAPPSSIGNFGGDSDNWMWP
                     RHTGDFSLFRIYSNQNNQPAEYSEDNVPYKPLYVLPISIKGVEENDFTFVFGFPGRTE
                     EYLPSYAIEMITQIQNPTRINLRRQKLDIYESFMTQDELVKLQYSNKQKGLANAWKKW
                     IGENNGIQKLNGIDKKKEYELRFQQWIDQSAERKEKYGNILSKLKDTYEILGGLQLEF
                     SYLLEAGLDIEIVKVARMTFGLKNLSEKEETTQDEIQKEIEKSKKQLLAFYKDYHQPI
                     DRQIFISMLQNYRKAIETKKSPAVFDIIDKKFKGDISLYADYVFKKSMFANRTTAISF
                     VENFKLSRLKRLIKDPGFQLANGLMDHYRNQLMPALSKTNSDKDSLMRLYMRAQMEFE
                     PERRFYPDANSTLRVAYGSVKSFYPRDAVFYEKQTTIEGIMEKEDPKNEDYIVDGRLK
                     TLYQTKDFGRYAHNGTLPICFIASNHTTGGNSGSPVFNGNGQLVGLNFDRVWEGTMSD
                     LMFDPEQCRNIMLDMRYCLFLIDKYAGARHLLDEMIVIE"
     gene            complement(13912..14289)
                     /locus_tag="PK345_03830"
     CDS             complement(13912..14289)
                     /locus_tag="PK345_03830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HPX34184.1"
                     /translation="MKKVIFTESAPKAVGPYSQAIEMNGHLFISGQVPIDPKTGKIVD
                     GGIKEQTDQVMKNIGEILKAAGYTYDNVVKSTCLLNDMENFTAMNEVYAKYYPNNPPA
                     RAAYGVVKLPLGAMVEIETIAMK"
     gene            complement(14355..17114)
                     /locus_tag="PK345_03835"
     CDS             complement(14355..17114)
                     /locus_tag="PK345_03835"
                     /inference="COORDINATES: protein motif:HMM:NF040057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative LPS assembly protein LptD"
                     /protein_id="HPX34185.1"
                     /translation="MRLSIQRGLYKCFKLLPCVIFLIISYWHFVSADIFAQETTYSKA
                     DSVTIKKPLIDTIGDFAKPISDTIFYQIFDEKPGESKDTTKVKAFDLETKVVYNSFEK
                     IHFDLRNRKVFLFYNAAITYGDIKLKANYIEIDFIKKQVYATGLPDSLGVIQGKPVFS
                     DGPQEFQADEITYNFDTRKGVIKHVMTQEGEGFIHGEKIKRLKDERINVSSGQYTTCN
                     LPHPHFEFRYGKAQLIPNKMIVSGPAYLAIEGVALPIAIPFGLFPNKSGQRSGIIIPR
                     WGESQNRGFYFERLGYYWGINDYMDFTITGDIFTGGSWAINPMYRYKKRYKYSGNFSF
                     NYAKNFTGDRDSPDRNEATDFSLIWSHNQDPKARPNSRFTANVNIYSRSFNQFNLSAT
                     DAYLSNTFQSSVSYQTNFNNAFFINLNALHQQNTIDGSVIVNLPTLSMNTKQFYPFRR
                     KEQIGQLKWYENITMKYSSSLENRIQTYDSLLFKPGWEKYFKNGMQHKIPISSSVKVL
                     KFFNFNNSAEYTERWYPYSIHRTWNPYDTIFGVNDTIYGRLTTDTLRSFVAARDFSYS
                     ASLNTRLYGMYQFSKGPVVALRHVLTPTISFNLRPDFGSKFWGYWDEVQVNDDGDMRR
                     YSHFEGLLYGGPPDGKSGNLSFSLTNNLEAKVRSRADTLNGTKKIVLIDNFTASMAYD
                     LAKDSLNLSPLRLSGRTTLFKQLNITYSSSWEPYATDENGKTINQLEWNINKKPFRMS
                     NTTWNFGINYRISSSDFGKKSGKAGEKSQVAVTEEPRPNEIIYQREVDDPHLYPDALI
                     NWDQPWSLNISYNLMFSNNPRFIKLYGWENNRNRVMTVGLTGDLSLTPKWKIGFRTGY
                     DFTSKQMSYTSIDFYRDLHCWEMRFNWTPLGFRKSWSFGINVKASILKDLKYDKKKDF
                     RDGFR"
     gene            17508..18455
                     /locus_tag="PK345_03840"
     CDS             17508..18455
                     /locus_tag="PK345_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014523.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HPX34186.1"
                     /translation="MKKISVEVKVGILFIIAVVLFIWGFNFIKGTDLFGGRRLFYAVY
                     DKVEGLEPANKVKVNGLNIGQVSHLGFIPGTSLICAELYIKSDLPIPKNSIARIYSTD
                     LLGGKAIEIILGDSDKLAVSGDTLQSELEISIREQVSEQVEPVQKKAMALINSLDSLL
                     MSIQGVFNPESQDNIISIFENIRVAIFSIKNSATLVDTLLAKEKSNLEKIISDIENIT
                     ANLRDNNDEISTIFRNFAILSDSLASADIPQTFREAQEAIENLKIISDKLNRGEGTMG
                     QLITNDTLYLNLQQSTENLNKLIEDIRLNPKKYVKFSVF"
     gene            complement(18488..>20364)
                     /locus_tag="PK345_03845"
     CDS             complement(18488..>20364)
                     /locus_tag="PK345_03845"
                     /inference="COORDINATES: protein motif:HMM:NF040138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPX34187.1"
                     /translation="PGTYNVSLTVSNAFGTDTKTKTDYIHVSTPSIVNFTADQTIINQ
                     GEVVHFSDLSTGNPYFWLWEFQGGTPAINYQQNPTIIFKTPGTFTVKLTVSYANGQAS
                     LTREDYITVNELVSLFPPGWDVTLTSTQHVIAVPLDANPRILETPIEPGDFIGVFFTN
                     TFGNLQCGGATMWDGQNNIAIISYGDQSYTPLKDGFSVYEDFKWKVYSYGHQREFDAT
                     PVYDVTLPNLGQFFPLGISALIDLSAGLLFNIHIPAGWSGISSPVLPFYPEMDTVLSS
                     IQDNLTILYNFDGIYWPWQNINTLDKWKNSGYTIKMSNTDNLVIGGELIKSLSQTIKS
                     GTGYLPIPTLYDVSTASLFAPFLDKLTLVRNILGTEVFWPQTGINTLPILKPGNAYIT
                     HAKSSFIVHFPQSGLKNSYSSPNSDFPELPLVSWSFAVASPDVHMISIPADGFKNLET
                     GDIVGVFTPNNVCAGWAVFENQPLAIPVYGTHETGDEKSGFMANEPFTFRIFRPFNQL
                     YCTAKALFDTTMPDQGIFRSGGISAISSLYPFDESFTNFSEEITVIPNPNNGIFVLSG
                     VSNIEEIVVISQEGQIIKTIENNGTSELNVDISDNRKGIYLIKIIEKQRVYVKKVVIN
                     "
BASE COUNT         5773 a         3951 c         3845 g         6785 t
ORIGIN      
        1 gttcttcttc aaatggtgaa tgtaggctga tggcaagatg gcattggctg tcgctgataa
       61 accgttttat ggctggaaca attccaatgg ttgatacagt aattcgtttc ggactccatc
      121 catagcccca atcggaagtt aagatttcga ggcttttcat aacttcatcc atgttgtcaa
      181 aaggctctcc catgcccata tatacaatgt tggaaagact ttttctttca gggatgctac
      241 gaatctgatt tagaatctgg ccggcggtta ggttagactg aaacccctgc ttgcctgtca
      301 tacaaaaaag gcaacccatc ttacagccta cctgcgatga tacgcaaagt gtgtttcgtg
      361 tctcttccgg aatataggca gcttcaatat atttgaaacg ggcaacttcg aaaaggtatt
      421 tttttgttcc atcagctgaa atttgtactt tcgaaggtgc aatcagtccc aaatcgaaat
      481 ttgcttcgag cttttttctg ttgtttaaag agatattggt catttcggaa aaggaaacga
      541 cctctttttg gtaaatccaa tctgctattt gcacagcagt gtaaacgggg agctctaact
      601 gtgatacaat ctcacgtaat tcgttaagcg tttttccaaa aagctgaggt ttaatattca
      661 tgttagatta aaaataaatt tcagcaatta tgttttctaa agaaataccc tttttaatca
      721 gcgcatctat ggtgtcaatt accatttcag ggcttccgca aaggtaatat cttctgtcat
      781 cgggtaatat actttgctcg ctgatccatt tattgatttt tccgaaaaag ataccattaa
      841 catgttcttg tgtacaacat cgtatgtaat tattacctaa aggtttaaat tcatcttcaa
      901 aataaaatga atgaagaaaa cgtccgctat gaattagtat tttatctttt tttaatcctg
      961 atctccacat cgaaacaaaa ggcgcgattc cggtaccggc tgctatccat actgccggtt
     1021 tagctgtacc gtaaaagtta ccggtaggta aagagcaaaa tagcgtatct cctggcttta
     1081 gttttgcaag ggctggagtt aggattccgc ttggtttaag gtcaaataat atttttacct
     1141 catcctctaa cattccactt gctatactgt aatagcgata ttgatcggca ggattcattg
     1201 caagagctac cacctgcccg ggtacaaaag aaaagctgcg gttgaaacta agtaaaaata
     1261 cattcggagc gatctcaatt aattgtgtaa ccttaattgg atgaagatca agcttttttg
     1321 agccggtgaa gtcaagcatt ggaattagag gtgatagcta atgtatgtat ataacccctt
     1381 agttgagttt ttgtttgcgt tgaacgaaag atagctgagc atgtcaacag gagaatacct
     1441 acacttgtga ggtggggaaa gtttgagtaa agtagaagtt attttataac tatgctttaa
     1501 aattcgatga tataattata gcttactttt tcgggcttgg ccttttccgg tgaagtttta
     1561 ggttttattt cctttttaac ctcatatttt atttcaaccg gaactttttg aggaagtttg
     1621 aaatttattc ttgttttcat tgctgcagtt actcttaaac cacggttttt ggctttttca
     1681 tattttaaca gccatatcaa cctgagagcc tcttcatcac agccatagcc taacccatga
     1741 ataactttgg cttcaacaac ttttcccaaa ccatttacgc gatattttat atgtactgct
     1801 ccttcaattt tattcttaag agcttcagga gggtatttta ggttctttcg gataaactct
     1861 tgaaaagctg ctttacctcc aggatattta ggaagtttga taaatttttt atccttttta
     1921 ggctcgctca tttttctgat ttgataataa taccaattat taatttatta ttaaacaacg
     1981 ctatgttttt tttaattgtg cggggctaat tgttgaatat ttttacttaa tgttatgcaa
     2041 ttgaaagaga aaacatattt taccatcggt tgacatacgc aaaaatagta cattttccga
     2101 gtcaacatgc aaacatatag caatttagtt atttttgcta ctcctttgat gtacatgaaa
     2161 agtattttca gttgattaat catacaaaaa cctatgattg atttttctgt aaccaatgaa
     2221 cacctcggta taactgataa atattgtggt tttgtcaacc gctggttagt tccgaatcat
     2281 ttgaattatg acgaaggccg gatgaatggt tcgatgggca aggaggatgg cggtcacggc
     2341 cagtctttgc ttgatgacgc gttggcttta gaagaattag gttccaactg cacagggata
     2401 gacatttgta ttgatgccaa tactccggcc tttacccctc tatatgttgc ggtttttgat
     2461 acgcttaaaa ataaaaatta aataatgatt tcgataattg ttgcgattgc cgaaaattat
     2521 gcaataggaa aagataaccg tcttttatgg catatttcgg aagatttgaa acgttttaaa
     2581 cgactaacca cgggcaacac ggtcatcatg ggcaggaaaa cctggttgtc gttgcctaag
     2641 cgtccgctac cgaatcggtt gaatgttgta ttgactaaaa acccgaagaa gtgttctgaa
     2701 ggctgtgtga tggttgattc cattgaggca gcgcttgaga tttgctctca ggctaaagag
     2761 tgttttatta tcggaggcga gactgtctat aggcaaatga tgcccttagc tgaccgacta
     2821 tatgttacca gaataaacaa gagttttgag gcagatactt tttttccgga gataagcccg
     2881 gaactttgga agttgacata cctgtcggag tggaaagaaa tggatgacaa gagctttagt
     2941 tacagatttg aaatatggga taaaaaggaa gattaatttt tttaattagg tatcctttaa
     3001 ctcttttcat ccggaattta ggagctacaa tcttcgatca agcaaattgg tgagtctatc
     3061 ttaatacaat cctccccaat tcattagaaa ccatgcttat tttctttctc agctttaatt
     3121 gagcatccat gaggatatca agattctctt gaatggtttc gttctgcatt tgcttaagat
     3181 tgtcttcata ttttgattcg agaacccgta acttgaatga aaggatagca tgggtaactg
     3241 agagttttaa tatctcctct tcaatattta cttttatttt tactttttcc cagtttccca
     3301 gttcatattt ttgagcaagc aagttggtgg ccattgtgga tatttgctta tcagaatgat
     3361 gaagatagaa gctttcggcc ggtatcgttc cgctttcaat ttgttcggaa gccgagtgaa
     3421 gtaccctttg atacaactca tttttcattc tgatttcatc attttcaaga tcttttgtaa
     3481 taaacaatgc aatacttact tcgaaggttt gtacctcttc atcttctgtc atagtttcaa
     3541 acacgatatt tcgattgccg tattgcatca acagacgtat gatttctttt tcatgatttt
     3601 ccgttgacac tgcctcttcc ccggggtgtt gtacatcagg gataatatat tgtggcagat
     3661 gggctaattc tgactcgcca tctttttgcc tctctttcag ctttttatca aactcctttc
     3721 gcctgatctg atttaatacg ttcattaacg tttgctcgcc gacatccatt aaggctgaac
     3781 attccttaac ataaaccgat cgggtgattt gatccggtat cagtgcaata gagcctacta
     3841 tctcttttat cagtgcagat tttttcaccg ggtcgccggc tgtttctttg agtagtagat
     3901 ttgttttgaa aataataaag tttaccgcct ggccttcgag gaaatcctgt acctcacttg
     3961 ttgtatgact ttgcgaaaat gaatccggat cttcaccctc agggaaaaag acaattttta
     4021 cgtttagacc ttgtccaagg atcatatcta tccctctgaa tgatgctttt attccagcct
     4081 cgtcgccatc gtaaagtatt gttatgtttt ttgtatagcg gcttataagt cttatttggt
     4141 cagttgtaag ggatgtgccg gaggaagcaa ctacattttc aaccttagac tgatgtaagg
     4201 agataacatc agtatagcct tcgacaaggt aacagttgtc ggtagcaatg atggccgatt
     4261 ttgctaaatg aataccataa aggatacggc ttttattgta aatttcagat tctggcgagt
     4321 tgacatattt cggaatattt ttatcggaaa gcagagtacg ccctccaaaa cctaagactc
     4381 ttcccgacag attatgaatt ggaaagacca cccgcccacg aaaccgatcg taactctttt
     4441 tatcttttgt tatggtaaga ccggttttct ccagatattc aagtttaaag ccttgttgta
     4501 aagcatgtgc tgtaaaattc tcccactcgt caatgctata gcccaattga aactttttga
     4561 tagtatcttc tctgaaacct ctctctttga aataacttaa tccaattgat cttcctgcat
     4621 cggtattaaa gagattatgg ataaagtatt tggaagcaaa atcgttgacc ataaaaagag
     4681 actctttttc ttgctgttct tcgattcttt cggcagtttg ttcttcatct tttatttcga
     4741 tgttatattt acgtgcaagg tatttaatag cttcaggata ggtaaaatgc tcatgttcca
     4801 ttacaaaatt caccgaatcg cctcctttgc cacaaccaaa gcatttataa atgccaagtc
     4861 gtggggttac ggtaaatgaa ggggtctttt cgttatgaaa cggacaaaga ccaatatacg
     4921 tggcccctcg ctttttaaga tttacaaact ccataacaac atcttcaatg cgcgcggtgt
     4981 caagaatttc ttgtatagtc tccttaggta tcaatttcgt tttaatgttt taaattttac
     5041 tgttgttttc aatgtagtgc cattcttact tgagaaacaa agttttaata gattgcttag
     5101 gtttataatc tctttgctgc atcaatcttt ataaagttca aatcgtctgt tttttttccg
     5161 aatctcatac aaaaatagta aaacgttcgg aagataaatt tgctatttac actgctaatt
     5221 gatcttgaaa ttatctttca gagtttttat ggtcaactat tgattttatg aaagtgattc
     5281 agattttata tttgttcagt acaattttta ttagatgcaa tctaaccgtt taatttagat
     5341 ttatcaaaaa aaatagaaat cctgcacttg ggaggtgcag gatttcagca gtaaattaat
     5401 catttatgga ggtgagaaaa ataacaattg ttgtttttgc aacaaatcta taaaacagct
     5461 acttatagtt tattgcaaaa taatcccaac aattttctgt aatatgcatt aagccaaagg
     5521 ctttctgagt ttatcttttt taatcgaatt ctttaaggat atctttaacc ttatcgggcg
     5581 aaacccgtcc aaaagttttt gtacctacag ttacaaccgg cgacagtccg caagctccaa
     5641 cgcatcgtaa gcaagtcaat gaaaatttac cgtcaggtgt tgattcccca acttttattc
     5701 ctaattcttt tttgaattcg tccaacactt tttcagcgcc acgcacgtaa catgctgtac
     5761 ctgtgcatat tgatattgga tatcttcctt tcggaatcat agtgaaaaac gaatagaaag
     5821 ttactacgcc atagacttta gctactgaga cattcatttc gcgtgcaaca acttcttgta
     5881 cttcagcagg tagatagccg aaaacgcctt gtgttttatg caatacgtta ataagttcac
     5941 caggcttgtt gccaaatgag ctagcaatct cttttatctg ctgaacgtct ttttccttta
     6001 gttttatttt tattgatgac atgatgttat ctctttgaaa gttattttgc gtttactcta
     6061 taatgatctc ttttttacga tcgaagtaat gggtatgtaa cagattatgc gatttttcgc
     6121 tgagcggttt ccccaaaaac tcttcataaa gacgaatgat gtacggattt tcatgggatt
     6181 tgcgcaatgt tttgttgaca tcctcttcat agacagcttt ttgtcgagct tttaatatcg
     6241 aagtatcacc gtgaatgtaa ggctgcccac cgccgccaat gcaaccaccc gggcaagcca
     6301 ttatttcaat ggcgtgatag gcgcattttc cttctattac ctcttctaat agtttccttg
     6361 cattacccaa tccatgagca atacctatat taagttcgag gccattaaag tcaacctttg
     6421 ctgaacgaat accctccatt ccacgtagtt gcttaaaatc aaccttgtca aggtgttttt
     6481 ttgtatagag ttcatacgtt gtacgtactg cagcttctat tactcctccc gttgtaccga
     6541 aaatcacact ggcaccggtt gattcaccaa atggattatc aaaatcgcca tccggtaaag
     6601 caatgaaatc aatgttcgca cgtttgatca aattagccaa ttctcgggtt gaaacagagt
     6661 aatctacgtc aggattacca tcaaccgaaa actcttcacg agtacattca tattttttgg
     6721 ctacgcaagg cattatggat acaaccacta aatttttacg gtcaacacca attttttcag
     6781 caaaataact ttttgctatt gagccgaaca tttgttgtgg tgagcgggct gttgaaggaa
     6841 tatctttcaa ttgtgggaac tgatgttcaa aaaacttaac ccaggcaggg caacacgagg
     6901 tcaggatagg gagttttaca tttttgtcgc cgtttaaaaa ttttgtaaga cggtcgagta
     6961 cttcggcgcc ctcttccata atggtgaggt cagcagcaaa gtcggtatca aaaacatagt
     7021 cgaatcctaa ctcgcgtaga gcagagacaa gttttcctgt gaccaaagtt cccggtttca
     7081 tgccaaaagc ttctcctaaa gcagcacgaa cggcaggtgc agtttgtaca atgacagtct
     7141 tgctggaatc ggcgaggtca tttattaact gattggtgtg atctacttcg gtaagtgcgc
     7201 cggttgggca aacatttaca cattgtccac aaaatgtaca ttcgctgtga tcgagattca
     7261 tttcgaaggc tggagcaact actgaggtaa aaccacggtt tattgccgag aggactccca
     7321 ctgtctgcac ttcattacac atcacttcgc agcggcgaca cataatacat ttatccacct
     7381 cccgaatgat ggaaggcgat gtgtcttcgc gataagttga ttgttcacct tggtatggaa
     7441 tatctctgat acccattgtt tgagctaact cctgcaattc gcaattgcct gattttgcac
     7501 atactaaaca atcgaaagga tgatctgaaa ggataagttg catcactgtt ttacgtgcat
     7561 ttaaaaccct tattgaatgg gtatttacaa ccataccttc agcacattcg gtagcgcaag
     7621 ctggagctaa gttacggcgg ccttctactt ctacaacaca aaccctacaa cctcctggtt
     7681 tattttcaat gttgaagtcg gacaaattca tgtagcatag ccgtgggata tgaattccga
     7741 tgctgtcggc agcctgtaaa atggtagttc cgggcttgac ttctactgtt ttcttatcta
     7801 tgattagctt tatcatattc aatttgtact aaatgatttg attaaacaat ttcgatggca
     7861 ttaaacttgc atttttcgta acatgcaccg catttgatgc aggctgtctg gtcaatctca
     7921 tgaacctgtt tgcgttcgcc tttaatacag ttaacagggc agtttcgtgc acacacagta
     7981 caacctacac agttttccgg gatcacaaca tagcgcatta gcgatttaca tacgctggct
     8041 ttacattttt tatcaacaac gtgctctaca tattcatccc aaaaattatt aatcgtcgag
     8101 agtactggat ttggagcagt ttgtcccaac ccgcaaagtg aagtatcttt aattacttta
     8161 cttaagtttt tcaacttgtc aagtgtttcc attgtgcctt cacctttagt gatagtgtca
     8221 agcatttcgt aaaggcgttt atttccgata cgacatgggc tgcactttcc gcatgattct
     8281 tcaaccgtaa agtctaggaa aaacttagct accgaaacca tacaatcgtc ttcgtccata
     8341 actatcatac ctccggagcc catcatcgag ccggctgcaa ccagtgtttc gtattctatc
     8401 ggtaagtcaa ggtgtttctc agtaaggcaa ccgccggatg gacctcctgt ttgaacagct
     8461 ttgaattttt ttccatcttt aataccgcct ccaatttcaa aaatcacttc acgtagtgtg
     8521 gttcccattg gaatttctat taaaccaaca ttattgattt ttccggcaag ggcaaatact
     8581 ttggtacctt ttgacttttc agttccaatg gtcttgaacc aatcggcgcc tttgttgata
     8641 atgattggaa cgtttgcaaa cgtctcgacg ttgtttacat tggttggttt gccaagataa
     8701 ccttctacag aaggaaatgg aggtttaaaa gtgggctctc cacgttctcc ttccattgaa
     8761 tggataagcg ctgtttcctc accacaaaca aatgctccag cgccgtatct aaggtcaata
     8821 tcaaaattga aacctgaatt gaataggttt ttacctaata atccatattc tctggcctgt
     8881 tcaatagcaa ttttcaggcg atgtatagcc agaggatatt cagcgcggat atatatcaga
     8941 cctttgtcgg ctccgatggc ataaccacat attgccattg cttcgagcac tacgtgcggg
     9001 tctccttcga ggattgaacg gtccatgaaa gctcccgggt cgccttcgtc ggcgttacat
     9061 accacatatt tctggtcgga cttactcttt cgtgtgatct cccatttaag tcctgtggga
     9121 aaacctgctc cgccacggcc gcgcaattct gattttttaa tcacatctat cgtagattca
     9181 ggagtcattt cggtgattac tttccccaaa gcctgatatc catcacgggc cagatattca
     9241 tcaatgtttt ccgggtcaat caagccacag tttcgaagtg ctattcgaat ctgtttgcgg
     9301 taaaacccca tgtgtttgct atcgggtaca tgctctttta cctttggatc agtatagagc
     9361 aggcgttcaa cttttcttcc tttgagtaca tgctctttta ctatttcttc ggcatcttcg
     9421 ggcttcacct caacataaaa ggtattgtcg ggatgaactt ttacgatagg gcctttttca
     9481 caaaaaccaa agcagccggt tgtaactact ttagcttcat cttcaagccc attggttttt
     9541 agttcacgtt cgagcgcctt ttgcagcgta gcgctttgcg atgcccggca gcccgtaccg
     9601 caacaaacca acaggtgcat tttaatttgt tttgccatag ttatattgca gaattttatt
     9661 cgtcaatagt ttcgtaattc accggaataa ttccttcaac cagctcgccg tttttgatgt
     9721 atttctcgat aatctcatcg gcctttttgg tatctacgta tccaaaaacg atcggatctt
     9781 cattagggcg ttttacctca atggtaggct cggcaaaaca ataccccata tcgcctgttt
     9841 gtgttaccac cgcatcaata ccacgttttc caagttcttc gatcaaataa tccataacct
     9901 ctttggcacc cgaagcaatc ccgctggtgg ccatacctac tttaatctga acatagtttt
     9961 ctggattaat acttgtctca cgtagtttaa gtttttgttc aaactcttct ttcttcttgc
    10021 gcagatcagc aagtgattta atttttgtca tgtgttgatt aactcctnnn nnnnnnnata
    10081 aacaataatt ggtaactgct aaaaaacaga tcattagcct tttattatta ggccgtttag
    10141 ctattttttt gcgtctgcaa aattaggact gttatttaac ctaaccaagt tttatgtgaa
    10201 aaaagtaaca gtgtgaaaaa aataacaatg aatttaaatt aagtctaatt tattgaatgt
    10261 tacgaaacta tgatttagag ttgtacttca gtcttttcag agttttgtct aagtatttct
    10321 ctgttatatt ttgttagaat gaaaagaaga ggtttaagga gttgtttcat gacaatttat
    10381 tgaaaaatac ggatgaattt tcaaattaat tagggaaata aaagcagttt attgatttca
    10441 ctcagttaat tcgctttttg ctttttcaaa ttcggtaatc atctcacgta taatttcacc
    10501 tgccggcatt atactgttga tcaaggccga tccttgtccg atttccaatt caccttcgtc
    10561 ggtattgcct tcgaacattc caagctttgc ccttcctttt cctaaatgag ccttaagttg
    10621 ttctatagta gctccgctcg cctgcatttc attaatttcg ttataaaact tgttttttat
    10681 caggcgcacc ggaagtaatc ttttcagggt gagttgagta tctccctctt ttgcttcaat
    10741 aattttttgt ttaaaaatag ggtgtgccga tgattctgtg gaaactacaa aacggcttcc
    10801 tacctgtacg ccttcggcgc ctaaagcgaa tgtggccaac atttgccttc ctgtgccaat
    10861 ccctccggct gctatcagcg gtagatccac cgcttttcga attatcggta ttaatactaa
    10921 tgttgtggtc tcttcaatcc cgttatgtcc tcctgcttca aatccctcgg caacgacagc
    10981 gtcaacacca gcgtcctgtg ctttgattgc aaatttagaa ctggcaacaa catgaacaac
    11041 ttttatccca tgttctttga atatgggtgt ccaggttttt ggattgcctg cagaggtgaa
    11101 tactattttt attttttctt ttaaaacaat ctccactatt ttgtctattt ccgggtataa
    11161 aagaggtatg tttactccaa atggtttttt tgtcgcagct ttagtctttt ggatgtgttc
    11221 ttttaaaaca tcggggtaca tcgaaccggc gccaatcaag ccaaggcctc ctgcttcgga
    11281 aacggcggca gcgagcctcc accccgaaca ccagatcatt ccagcctgaa taattgggta
    11341 gcggatatcg aaaagttctg ttattcttgt tttcattgca aagagttata tgaatttttt
    11401 ttaaaatcaa ttattgttag gctaaaataa ggtgatatta gcttggtcaa tcttatttgg
    11461 gattttacca atacttgata tgttttattc aataactatc atctcatcca aaagatggcg
    11521 tgctccagcg tatttatcaa taagaaaaag gcagtatcgc atatcgagca taatgtttct
    11581 acactgttca ggatcgaaca tcaggtcgct catagtgcct tcccacactc ggtcgaagtt
    11641 gagaccgaca agttgcccgt tgccattaaa taccgggctt ccggaattgc ctcctgttgt
    11701 atgattggaa gcgataaagc aaattggtaa tgttccgtta tgcgcatatc tgccaaagtc
    11761 ctttgtttga taaagtgttt tcagcctgcc atccacgatg taatcttcat ttttagggtc
    11821 ttctttttcc ataatccctt caatggtagt ttgtttctca taaaaaaccg catctcgcgg
    11881 ataaaaagat tttacactac cgtaagctac tcttaatgtt gaattagcat caggataaaa
    11941 tctgcgttcc ggttcgaatt ccatttgagc cctcatgtaa agtctcatca ggctgtcttt
    12001 atcgctgtta gttttggata gagcaggcat aagttggtta cgatagtgat ccattagtcc
    12061 attggcaagt tgaaaaccag gatctttaat taatctcttc aatcgtgaaa gcttaaaatt
    12121 ctcaacaaaa gatatagctg ttgttctatt ggcaaacatt gattttttaa aaacataatc
    12181 ggcataaaga gagatgtcgc ctttgaactt tttatcaata atatcaaaaa ccgccggtga
    12241 ttttttggtt tcgatagctt tcctatagtt ttgcagcata gagataaaga tttgcctgtc
    12301 aataggctga tgataatctt tgtaaaatgc taggagttgc tttttcgact tttctatctc
    12361 cttctgaatt tcatcttggg ttgtctcttc tttttctgaa aggtttttta atccgaatgt
    12421 catgcgtgct accttgacga tttcgatatc gagcccggct tcgagcagat aagagaattc
    12481 aagttgaagc ccgcccaaaa tttcataagt atctttaagc tttgataaaa tattgccata
    12541 tttttctttt ctttcggcag actggtcaat ccattgttgg aagcgcaatt catactcctt
    12601 ttttttatca attccattaa gtttttggat tccgttattc tcgcctatcc attttttcca
    12661 ggcatttgcc aacccttttt gtttgtttga atactggagt ttaactaatt catcctgcgt
    12721 cataaatgat tcgtatatat cgagcttttg tcttctgaga ttaatacggg tagggttttg
    12781 aatctgtgtt atcatctcga tggcgtacga gggcaggtac tcttcagtgc gtccaggaaa
    12841 cccaaagacg aaagtaaaat cattttcctc aacgccttta atagagattg gcaatacata
    12901 aagaggcttg tatggtacat tgtcctcaga gtactctgca ggctggttat tctggtttga
    12961 atatattcta aaaagcgaaa aatcgcctgt gtgtcttggc cacatccagt tatcgctatc
    13021 tccaccaaaa ttaccaatac tcgagggggg cgctccaact aatcttatgt ctttaaagat
    13081 ttcatttaca aaaagataat attgatttcc ataaaaaaaa ggctttacga ctgcttcgta
    13141 atgggtatct ttcgtggctt catctttaat ttccttgata tttttttcta tgatttcggc
    13201 acgggcttct tctgtaaggt catcagttac ccctgataaa gcggcggcag taacgtcttc
    13261 catctttacc aatcgcgtaa cggtaagtcc aggacaataa agctcatctt gccgcccaac
    13321 tgcccaaaag ccattggtta acatatcgtt ttcaatagtg ctaagacgtt gaatagcgcc
    13381 atatccgcaa tgatggttgg taagtaataa accttcagca gagatagctg aagccgtaca
    13441 gcctcctccg aaaaggaaaa tagcatcctt catggagctg ttattgatat cgtatatatc
    13501 gctggctgta atgcgcatac ctaacctact catatctttt tcattcatct tttcgagtag
    13561 cataggtatc cacattcctt catcggcaaa agagatgaat ggaaagagga taagtaataa
    13621 tagaataaga cggttaaaaa tagacttcat gatcaaattt ttattttttt aatgttaaat
    13681 taatattgat agtgtaaaat tattgatccg gcaaataact ttttaaaaat caccggtttg
    13741 tgtattgtgt aatgtttatt gccaagccaa aaaccgattc cggctaaaga agtacatcaa
    13801 tacatttgaa acgcctctga attgctgaat agcaattact tgatgcttta catattgaaa
    13861 gtaaaataat cccttaccag aatttaaggt agtcagcgtc gggatgtttt attatttcat
    13921 ggctattgtt tcaatttcta ccattgcacc taatggtaac ttaacaactc cgtaagctgc
    13981 acgtgcaggt ggattatttg ggtaatattt agcataaacc tcgttcatgg cggtaaaatt
    14041 ttccatatcg ttgagtaggc aggttgattt gacaacatta tcgtaagtat agcctgctgc
    14101 tttaagtatt tcgccaatat ttttcattac ttgatcggtc tgttctttaa tcccgccatc
    14161 cactattttt ccggtttttg gatcaatggg cacttggccg gagataaaaa gatgtccatt
    14221 catttcgatt gcctggctgt atgggcctac cgccttcggg gcgctttcgg tgaaaattac
    14281 ttttttcata acgttttgat attaaattta atagttgata ctttgtttta cataatcctt
    14341 atttgccaaa attattatct aaatccgtct ctaaaatctt ttttcttatc atatttcagg
    14401 tcttttaata tagaagcttt tacgttaata ccgaaactcc agcttttcct gaatccaagt
    14461 ggtgtccagt taaaacgcat ttcccagcaa tggaggtcgc gatagaagtc aatagaggta
    14521 tatgacatct gttttgatgt gaagtcgtag ccggtacgaa atcctatttt ccactttggc
    14581 gtaaggctca aatcgccagt aagcccaacg gtcattaccc tgtttcgatt attctcccag
    14641 ccgtaaagtt taataaaacg tgggttgtta ctaaacatca ggttataact aatatttaag
    14701 ctccatggtt gatcccagtt aattaacgca tcgggataaa ggtgtggatc atcaacttca
    14761 cgctgataga ttatttcatt tgggcgtggc tcttcagtta cagcgacctg tgacttttct
    14821 cctgccttac ctgacttttt tccaaaatct gaagagctta tcctatagtt gattccaaaa
    14881 ttccaagttg tgttactcat tcgaaagggc tttttgttga tgttccattc gagctggttt
    14941 atagtttttc cgttctcatc agtggcataa ggttcccaac tacttgaata ggtaatattc
    15001 aattgcttga atagggttgt tcggccacta agccttaatg gcgacaagtt gagcgagtct
    15061 tttgccagat cataagccat tgaagcagtg aaattatcaa ttaaaactat cttttttgtt
    15121 ccatttaagg tgtctgctct ggatctgact tttgcctcaa ggttgtttgt taaggagaag
    15181 gataggttac cggatttccc atcgggcggg cctccatata gcaatccctc gaagtgtgaa
    15241 tagcggcgca tgtcgccatc atcgtttacc tgaacttcat cccaataccc ccaaaatttg
    15301 gatccaaaat ctggtcgaag attaaaactg atagtggggg ttagtacatg ccttagtgca
    15361 acgaccggcc ctttggaaaa ctgatacatt ccatatagcc gcgtgtttaa tgatgctgaa
    15421 taggaaaaat cccgcgcagc tacaaacgat ctgagcgtat cggtagttaa tctgccataa
    15481 atggtatcat tgacaccaaa aatggtgtcg taaggattcc atgtacgatg gattgaatat
    15541 ggataccatc tctcagtata ttctgccgaa ttgttgaaat taaaaaactt aagcactttt
    15601 acggaactgc taatgggtat tttgtgctgc ataccatttt tgaaatactt ctcccaaccg
    15661 ggtttaaaga gcaaagaatc gtaggtttga attctgtttt cgagcgatga tgagtacttc
    15721 atcgtgatgt tttcgtacca tttcaactgt cctatttgtt cttttcgcct gaaaggataa
    15781 aactgctttg tgttcatcga gagcgtagga aggtttacaa tgactgagcc atcaatggtg
    15841 ttttgttggt gaagtgcgtt taaattgata aaaaaagcat tgttgaagtt cgtctgatac
    15901 gaaacactcg actgaaaggt attactcaaa taggcatccg ttgcagagag gttgaattga
    15961 ttaaagctac ggctataaat gtttacatta gctgtaaagc gtgaattggg tcgtgctttt
    16021 ggatcttggt tatggctcca tattagcgag aaatcagtag cttcattacg gtcgggggag
    16081 tctctgtcgc cagtaaaatt tttagcatag ttaaaagaaa aattaccact gtatttatat
    16141 ctctttttgt acctgtacat gggatttatg gcccagcttc cgccagtaaa aatgtcgccg
    16201 gtaatggtaa aatccatata atcgtttata ccccagtaat aacctaaccg ttcgaaataa
    16261 aaacctctat tttgcgattc accccatcgt gggataatga ttccggatcg ttggccactt
    16321 ttgttgggaa ataacccaaa tgggattgca ataggtaggg caacaccctc gatagccaaa
    16381 taagccggcc cggaaacgat cattttgtta ggtatcaatt gtgctttacc atagcgaaat
    16441 tcgaaatgcg gatgtggcag attacacgtt gtatattgtc cactggatac attaatacgc
    16501 tcatccttta accgtttgat tttttctccg tggataaagc cttctccctc ctgcgtcata
    16561 acatgtttaa taaccccttt gcgagtatca aaattataag taatctcatc agcctgaaat
    16621 tcttggggac catccgaaaa aaccggttta ccctgtatca ccccaaggct atcgggtaat
    16681 cctgttgcat aaacctgttt ttttataaaa tcaatttcta tatagttggc tttcagtttt
    16741 atatcaccgt aggtaatagc agcattgtaa aacagaaaaa cctttctgtt tcttagatca
    16801 aaatgtattt tttcgaatga attatataca accttagtct ccagatcaaa ggctttcacc
    16861 ttagtcgtat ctttcgattc accgggcttt tcgtcaaata tttggtagaa tatagtatcg
    16921 gatattggtt ttgcaaaatc acctatggtg tcaatcaatg gttttttaat tgttaccgaa
    16981 tctgcttttg aataggtcgt ctcttgggca aaaatatctg ctgatacaaa atgccaatag
    17041 gaaatgatta aaaaaataac gcaaggtaaa agcttaaaac atttgtataa gcccctttgg
    17101 attgatagtc tcaaaggaag aagagaattg gatatcacta tttggcgttt caaaccgaag
    17161 tatgattatc agcttaaata attatttgca aagtaaaaga atttaggttt tgatcaatgg
    17221 tatttaatag ttaacaggac taatttaaca gggtttatta cttctatttt attaagtaga
    17281 ttagttaatg ctgatttatg aaacgagtat ctttagtttt tcttgttttc attttttttg
    17341 taacatcaat ttatttttta ctaagcgttg taatttaaac aggattagaa cttcataatt
    17401 aataaaatga aattaattga agcagaataa gataatgtta tttaataatt gattttgctg
    17461 ggtgattatt tttttaagta gctttgtaca ttcaaattag aatgctcgtg aaaaaaattt
    17521 cggttgaagt aaaagttgga atacttttta ttatagcggt tgtgctattt atttggggtt
    17581 tcaactttat taaaggaact gatcttttcg gcggacgaag gctgttttat gctgtttacg
    17641 ataaggtgga ggggcttgaa cctgccaata aagtaaaagt taatggttta aatatcggtc
    17701 aggtaagtca tttaggattt atacccggca catcactcat ctgcgccgag ctttacataa
    17761 aaagcgatct acccattcca aaaaattcaa tagcccgcat ttacagtact gatttgctgg
    17821 gcggtaaggc cattgaaatc atacttggcg actctgataa actggctgtc agcggcgaca
    17881 ctcttcaatc ggagttagaa atatcaatca gagagcaggt gagtgagcag gttgagccgg
    17941 ttcagaaaaa agcaatggcc ctaatcaatt ccctcgactc attgcttatg agcattcagg
    18001 gtgtctttaa cccggaatcg caggataata tcataagtat ctttgaaaat atcagagtag
    18061 caatatttag cataaaaaat tctgcaacgt tggtggatac tcttcttgca aaagaaaaat
    18121 ccaatcttga aaagataata agcgatattg aaaatatcac tgcaaacctt cgcgacaaca
    18181 atgacgaaat cagtactatt ttcaggaatt ttgcaattct ctccgattct cttgcatctg
    18241 ccgacattcc acagacattt agagaggctc aggaggccat tgaaaatctt aaaattatta
    18301 gcgacaaatt aaatcgaggc gagggtacaa tggggcagct tattactaat gacacactct
    18361 atttgaacct gcaacaatca actgaaaatc ttaataagct aatcgaggat atacgcttaa
    18421 acccaaaaaa atatgtaaag ttttcggtgt tttgatgctt aaaaaaatgt gatcttagga
    18481 ggttcggtta atttattaca acttttttta cgtaaaccct ttgtttttca atgattttga
    18541 taaggtagat tcctttacgg ttgtcactta tatcaacatt taattcagaa gttccgttat
    18601 tctcaatggt ttttattatt tggccttcct gcgagattac tactatctct tcaatattac
    18661 ttacaccgct taaaacgaat attccattgt tcggattagg tataacggta atttcttcag
    18721 aaaaattcgt aaaagactca tcgaatggat ataaagaact aatagccgat attccacctg
    18781 atctgaagat accctgatca ggcattgttg tatcaaataa agcttttgct gtgcagtaaa
    18841 gctggttaaa tggcctaaaa atcctgaatg tgaatggttc attagccata aatccggatt
    18901 tttcatcacc tgtttcatgg gttccataaa ctgggattgc caaaggttga ttttcaaaaa
    18961 cagcccatcc ggcacaaaca ttattcggtg taaaaacacc aactatatca cctgtctcaa
    19021 ggtttttaaa accatcggca ggaatcgaga tcatgtgcac atcaggagat gcgacggcaa
    19081 aactccatga aactaatggt aattcaggaa aatcgctgtt cggactggag tatgagtttt
    19141 taagacctga ctgaggaaaa tgtacaataa agctgctttt agcatgagtt atgtatgcgt
    19201 tgccaggctt tagtataggt aaagtattta tccctgtctg aggccaaaaa acttctgttc
    19261 ccaaaatatt gcgaaccaga gttaatttgt ctagaaaagg agcaaaaaga gaagcagtag
    19321 agacatcata aagagtagga ataggaagat atcccgtccc tgattttatg gtttgagata
    19381 acgacttaat taattcccct ccaataacaa ggttgtcggt atttgacatt ttaatcgtat
    19441 atcccgaatt tttccattta tccaaagtgt ttatgttttg ccagggccaa tagatcccgt
    19501 caaaattgta gaggattgta aggttatcct gaatagacga tagaacagta tccatttccg
    19561 gatagaaggg taaaacaggg cttgatattc cgctccatcc tgctggtata tgaatgttga
    19621 agagaagccc agcgcttagg tcaattaagg ctgatattcc taatggaaaa aattgcccta
    19681 aatttggtaa cgtaacatca taaacaggtg tcgcatcaaa ttctctttga tgaccgtatg
    19741 aatatacttt ccacttaaaa tcttcatata cagaaaagcc atctttaagt ggggtatatg
    19801 attgatcccc ataggagatt attgctatat tattctgacc atcccacatt gttgctcctc
    19861 cgcattgtaa atttccaaaa gtgttggtga agaaaacccc tataaaatcc cctggctcaa
    19921 tgggtgtttc taaaatccgc gggtttgcgt ctaaaggcac ggcaattaca tgttgtgtag
    19981 aggttaaagt aacatcccag cccggtggaa acaacgaaac taattcgttt acagtaatat
    20041 aatcttcacg agttaaggaa gcttgtccat ttgcatacga aacggttaat tttacagtaa
    20101 atgttccagg agttttaaaa atgatagtcg ggttttgctg ataattaata gctggtgttc
    20161 caccctgaaa ttcccataac cagaagtagg gatttccggt cgaaaggtcg ctaaagtgaa
    20221 cgacttctcc ctgatttatg attgtttgat ctgcagtgaa attaacaata gaaggagtgc
    20281 tgacatgaat ataatctgtt ttggttttag tatcggttcc gaaagcgttt gatactgtta
    20341 ggcttacatt ataagttccg ggag
//
LOCUS       DAOOAI010000034        20003 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_3573_length_20003_cov_5.588681, whole genome shotgun sequence.
ACCESSION   DAOOAI010000034 DAOOAI010000000
VERSION     DAOOAI010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 20003)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20003)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20003
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3573_length_20003_cov_5.588681"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            273..6326
                     /locus_tag="PK345_03850"
     CDS             273..6326
                     /locus_tag="PK345_03850"
                     /inference="COORDINATES: protein motif:HMM:NF036531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HPX34188.1"
                     /translation="MKLLKLAFFIAILSAIVLFITANIPPMDKNYDDKWKQVDSLVSL
                     NQPRSALELVNEIYKIAQNEQNSPQLIKANIYRIKLMADYEEDYLVKAISEIKTEIKS
                     AGFPEKQVLHSLLGDLYLRYFHTNRYQILERSKVAGETSDDIQTWDAAKLLSTAASHF
                     EVSLSPANQLQGVDLKAFSAILLEEKDSKIFRPTLYDFLAHRAIDFYASGETGITTAA
                     DQWKPDHLNYFNSVESFINLQLPENIAGTFSGKILAIYQELLLFHLNDKDVQALIDVD
                     LKRLNYVHSNTIITEKDGLYQNALNALLKKFNNSPYSTAIAFELAMFHNTKASEYNPN
                     IADDYRWERKKASDICQEAIKKFPNTTGAANCLILLDDMIKPSLSIQTEIATTAEKPS
                     LGLIKWKNYNKVWFRLIAIDYKEYQELSWRVPIADLATKLIKLPALENWSIDLPDESD
                     LQQHTTEFKIPALQNGFYILLASGNKDFSGKDHQLAWTNLWITNLTFIHKKLESGGVE
                     FFVLDRIKGTPKSGVKYKAYARVYDFKNREYNDKLLASGTSDRNGYISILPSQQSANT
                     SITIELSEKKDRFFPETSFYLGPTIKTDPKPEIKTYFFTDRAIYRPGQTVYFKGIIIE
                     KTGDEQKVKANYETKVDFFDVNFQLISSLTLTTNEFGSVNGAFIIPTGVLNGMMTIRN
                     SSGGTTFNVEDYKRPTFEVDFLPIQDNFKLNTEISVEGEVKAFAGYMIGGANVKYNVV
                     RRNMFPYRWLSYMDFFPRSSETEITTGETQADENGKFYIKFPAIPDQQINRRFKPVFS
                     YTVNVTATDINGETQSGSQMIIVGYEALLLDIDIPEKLDRNQVKSFNLETENLAGVRQ
                     KTIVSVEVSRLIDPGKLLKERLWEQPDQFIIPSDQFIKEFPEHTFKDELSHDNWAVAE
                     TVFSKNINTATDSLLRFDNLSNWKSGKYVVVLKATDNFGTQVETKKYFDLFSLTDRQP
                     PVTVYNWFVPLKSSGEPGEEATFLIGSAAQRVKALYEVQHHGRTISRQWISLEKEQKV
                     ITVPIKEDYRGGFTIQLTFVVNNREFNNRMWVTVPFTNKQLDLTFETFRNVLEPGGKE
                     EWRVTVKDKKGDQAAAQMLASMFDASLEAFVPHNWNFNLYIPNYLSNPWQLSGSIGTS
                     GGNNWDFTLRSHKSFHFNTYDRLNWFGFNGFRISDSFFYKSGAMSRDMVQMNTQELAV
                     VEESTLIVDAYDDGKLAEMETAEEKAEKAKRIEPSLLRRDFNETAFFYPELQTNDKGE
                     VIIKFSLPESFTKWKFMALANTRDLMTGMMEQEFTATKKLMVMPNVPRFLRTGDTLQF
                     TARITNLSEELLTGNASLEFFDPITEKNITEILKTKTKQGTFSVAAGKTTSVSWNITV
                     PEDFGIISYRIKATAGNFSDGEEKSVPVVPSRILVTESLPLPITGASSKAFEFNKLVE
                     SGKNTTIKHQSLTLEFSTNPAWYAIQALPVISEPIHKNSHSLFAAFYANSIAYYISNA
                     NPKIKNVFESWKSQTPEAFLSNLEKNQDLKAIILEQTPWLLQARNESERKQRIALLFD
                     LNNMRDRLDASLKALIQIQSPNGGFPWFEGMPDSRYITQNIVEGLGKLQSVRILSAVA
                     DERVEQMITKAIRYLEERNKEDYQYILSRRKDKIEEDNLSDIHIQYLYARSFFPHIKM
                     NPSCETAFNYFKNQSEKYWAKRNIYLQGMIALTLQRYGNNNIPPLIVKSLKERSLKNE
                     EMGMYWRHESGYYWYQAPLESQAMMIAVFDEVADDKEAVEMMKIWLLKQKQTQDWKST
                     RATVDAIYALMKKGKDLLTEPEKVKIEVNGKELLMNEVGKREAGTGYFQKTWNSPEII
                     PQMGNIKITKTDDGIAWGAMYWQYFENLDKIAPHKTPLSITKELFVEKRGTKGTVIEK
                     LNDEAKLKIGDKVIVRIELRVDRNMEYVHMQDMRASALEPVNVLSSYKYRGGLGYYES
                     TRDAASHFFFDFLPKGTYLFEYPLIASQIGSFSNGITTIQCMYAPEFTSHSEGIRIVV
                     E"
     gene            6398..6988
                     /locus_tag="PK345_03855"
     CDS             6398..6988
                     /locus_tag="PK345_03855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HPX34189.1"
                     /translation="MESICVFCGSSVGKRPEYSETARSFGKLIASRDITLVYGGGNIG
                     LMREVADGALEAGGKVIGVMPKLIVDKEIAHNSLHELKIVNTMHERKALMAKLSDGFV
                     ALPGGFGTIDELFEIMTWNQLGIINKPCGLLNIEGYFNHLLEYISCAVNERFVRPEHQ
                     ANLIVDNDPEELIDRLEKYRPQVAEKWIERLKVDLI"
     gene            6998..7552
                     /locus_tag="PK345_03860"
     CDS             6998..7552
                     /locus_tag="PK345_03860"
                     /inference="COORDINATES: protein motif:HMM:NF024635.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HPX34190.1"
                     /translation="MIVIGITGTLGAGKGTIVDYLVKNKGFNHFSVRSFIAQEIQNRG
                     MTVNRDSLTEVANDLRSANSPSYIVDALYSQALRIGKNSIIESIRTQGEVESLRANGN
                     FYLFAVDAPAEIRFKRVKLRGSETDNIDFQTFLQNEKREMTTIDPNKQNLKKCIEMAD
                     FIFENSGTIAQLEERVKKVIEKLL"
     gene            7682..8185
                     /locus_tag="PK345_03865"
     CDS             7682..8185
                     /locus_tag="PK345_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018051019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HPX34191.1"
                     /translation="MEQSSSSKGYQRPTWDEYFMELANTAAKRATCDRGRSGCVIVRD
                     KHVLVTGYVGSPLGLPHCDDVGHLLKKVYHEDGRITQHCVRTVHAEQNAICQAARLGI
                     ALDGGTLYCKMTPCHTCAMLIINCGIKRVVCERKYHAGEESERMFRQAGVIIDFFNDE
                     VEQYDNQ"
     gene            complement(8233..9906)
                     /locus_tag="PK345_03870"
     CDS             complement(8233..9906)
                     /locus_tag="PK345_03870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34192.1"
                     /translation="MHSKILFKQLFSILLLAVIPVVLIFIGINLRLANQNYSMFNIDP
                     EFILLFSGIALGHGSINLFIDQPGTPVIVFAAIIMRIIHLFRPSSSFTQDVLQNPDFY
                     LNALNISMIVFITLIIFFAGWCIYRKTGNLPAAIFIQFSPFVVEHVISIIERFMPEPW
                     FIAHVIVLCSITVLDLYGYFNNTKLKYQSWLVYGVIIGFGISLKLTFLPFFLAPLFLL
                     TGIRKKSYYLGTTALSFLIFTFPIFKRSREFFRWTKALAINSGRYGSGKASVIDPDQF
                     FIQLKAIIQSEKYLVYITILALLVLIISLLPWVRRRIGNKRYIAALAGFTATLIVGIL
                     IISKQYAGHYLVPYSLLTVFICYLSINVLIEIVQFRKWWLEAIIYSLVALMMLSNPKS
                     IKQHKKYLEIRRQNLSYKKEIVTEIEKLPQSDVLILSADNWHFKKEAGLIFGMLMTPR
                     GVKKYGEQLNTLYPNTCLFKESEGKFFNWLDKPFTASELLVAFSKITVMVKHNNEAVF
                     KEIEDVFEETEKADVEIIYSDPKTNLTIYEIRRVDSGLSAEYYLPPGSI"
     gene            10226..11932
                     /locus_tag="PK345_03875"
     CDS             10226..11932
                     /locus_tag="PK345_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004290013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004819 - glutamine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006425 - glutaminyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase/YqeY domain fusion
                     protein"
                     /protein_id="HPX34193.1"
                     /translation="MLTEEKNQENLETRSSVNFIEAIINEDLRLGKNNSRVHTRFPPE
                     PNGYLHIGHAKSILLNYGIACKYNGKFNLRFDDTNPIKEEQEYVDSIMEDVRWLGADW
                     EDRLFYASDYFDQLYDWAEKMILDGKAYVDDQSAEVIAEQRGVPTAPGIESPYRNRLP
                     EENLDLFRRMKAGEFQNGERVLRAKIDMASPNMHMRDPVMYRILHAYHHRTGNQWCIY
                     PMYDWAHGESDYLEGITHSICTLEFEVHRPLYDWFVDQIQETDYRPRQIEFARLNLSY
                     TIMSKRKLLELVKEGYVNGWDDPRMPTISGLRRRGYTPEAIKVFAEKVGVAKRDNVID
                     VSLLEFSIRENLNKTAKRVMAVLNPVKLTITNYPQDLTEDLITENNPEDPDAGMRTIP
                     FSKELFIERDDFMISPPKKYFRLSPGKEVRLKSAYIIKCNDYRLDERGEVVEIFCTYD
                     PESKSGGAGSNRKVKGTLHWVTVAHAIDAEVRLFDRLFKVENPEDVAEGEDYKTNINP
                     DSLKVLNQCKLEPIMANAIPGEKFQFQRIGYFTVDPDTTPDKLIFNRTVPLKDSWSKS
                     AL"
     gene            complement(12227..14350)
                     /locus_tag="PK345_03880"
     CDS             complement(12227..14350)
                     /locus_tag="PK345_03880"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HPX34194.1"
                     /translation="MAVIGKIRKHSGLLVVVVGIALAAFVLGDFLRPSGNRPITIIGK
                     IDGTEIPIQNYNDRVAEQLENLRAQQQTERVPAEEEFQIRQQVWNQIIEETILNDVYK
                     KIGLIVTKEELSNEIIGDNPNRYVQQSFRDPNSETFDPEVVRNFLKNLNQQGPEMKRR
                     YLSLERMVKDDIIRTKMKNLLSKNYHIPQPFAKLDYQIKNQNADVIYVAAKYSDVADS
                     LINVTDNDLRNYYNDHIYNYRQEDSRTFDYVLFEILPTQEDRQDVKEEVDRLYVQFKD
                     AEDPAAFINSVSDTRFDSTYKKESDLPISLAESIMSAKIDTVFPPYIEGDTHHIIKVL
                     DFQQRPDSIEMSQMLVSYSTARAGFQLNERSEEEAKVLVDSILTALKKAPSIFEELAL
                     KYSDYPSVAEDKGALGWMTDGDRSFTNFFKEGVSLKVNEPDTMETGLGIHVLLVTGKT
                     ALIPKVKIGQITRTVEPSSQTYRQVFSQANKFASENRTLAKFDTAVVNQGLNKRTAER
                     ISSTSNRIPGVEQSRQIIRWAFWETTKVGDVSTIFEDDKQFVIAALKEIKNKGYSPFE
                     QVKEQIRPLVVNKLKGEYLVKKINDFNATTLNDLASNMNSKVDTAKNLSFSARNIPGF
                     GSEPNVIGQMFTVEPKQISKPIAGYNAVFVAMIDRLTPAPEVNDFGTTIFQLRNSFES
                     RVNSNAFIRALEKESKIEDNRLMFY"
     gene            complement(14435..15712)
                     /locus_tag="PK345_03885"
     CDS             complement(14435..15712)
                     /locus_tag="PK345_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HPX34195.1"
                     /translation="MYTYIYIIIVLIFSAFFSGMEIAFISANRLKIEVDKNNGSFSAR
                     ILSRFIRIPSKFIGAMLIGNNVVLVVYGILMAQVLKDPIDKILPPIIRSDFSILLSQT
                     LIATIIVLIVAEFLPKVLFRINPNQILSFFAVPVTILYYLLYPVIQVYIGFSEFIVKR
                     FSNIKFSHDHLVFSLIDLDHYLKEYSAEADKINEAATQEIQMFQNAIDFRNVKLRECM
                     VPRTEIIALEENDPINKLRDSFIKSGHSKIPIYRDSIDNIIGYTYSADLFKNPKSIKS
                     ILRTIIVVPEAMLASTALTTFIQGNKSIAVVVDEFGGTSGLVTIEDVIEEIFGEIDDE
                     FDVEQLIEKKISDHEYIFSARLEIDYLNDHYDLNLPESEEYETLGGLIIFHHQSIPNL
                     HEKIVINNLEFNILEAAINRIETINLKIVTDSE"
     gene            complement(15712..16299)
                     /gene="lptC"
                     /locus_tag="PK345_03890"
     CDS             complement(15712..16299)
                     /gene="lptC"
                     /locus_tag="PK345_03890"
                     /inference="COORDINATES: protein motif:HMM:TIGR04409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HPX34196.1"
                     /translation="MSAKPNKYYLRTLTKTIALIICIGVTTYSCKNDMKTITSLTLEE
                     NIPLETAKNIEAVYSDSGKIILKIVSPLLNRYIKEDHYLEFPEGLTLYFYDSMMNVKA
                     TLTANYGVSWEETNIMEVKDDVVINNYDKQEVLNTEHIIWNRKEQKIFSNVFVKRTSP
                     DGVLYGDGFDANESLTNYTLRNPHGVFTIQDNEPE"
     gene            complement(16327..17727)
                     /locus_tag="PK345_03895"
     CDS             complement(16327..17727)
                     /locus_tag="PK345_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX34197.1"
                     /translation="MKELIKIVTISLILLLAFSSLINAENSLRTNANTPATVILLPDN
                     PQKYGEDSVKCVTNLSLYREFYKQWRASEFTSSTVNDAIGPWRWVFFNCPAASQYTYI
                     DGPVMIEHFLRNEKSEDKKDKYVDTLMMIYDQRIENFGREGYILGRKGTDLLKYRPDA
                     FKEAYPIFKRSVELQGNESESFVLAYYFRSVTQMADENLIEKITVLEVYDELNQIMDY
                     NIEANKDDADVVASWQNIKGSIDVAFEPYATCEDLVSIYQPKFDENPEDINLLRKITK
                     MLDNKGCTDTQLFFDASIQLNKLDPSPTSNLMIGKMLIKREKYSESLPYLEKSIEDNN
                     EDQKADILFLISKVHQQLKNYSTSRRYALQSLELKPNNGNILISIGDMYASSSSECGD
                     NELTKKVVYWVAVDKYVRAKQIDPEVAEIAQSRIAIYSQQFPSSETIFFYNLTEGDDY
                     TVECWINEKTKVRAFK"
     gene            complement(17784..19070)
                     /locus_tag="PK345_03900"
     CDS             complement(17784..19070)
                     /locus_tag="PK345_03900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34198.1"
                     /translation="MLTGSSKGFKKLSLLIFLIFSLAGYSQVDVNSPYSRFGIGELSA
                     SNSTYSFSMGGISNALSSPFHVNLNNPASNANFDTLSFVFEGGLSSKFGSISTKTLSG
                     SSNYASLGYLLFGFPVSGRIKVSIGLTPYSNLGYKIKNTELVPEIGNSEFAFEGTGGV
                     NRFFIGASLKLHNNLAIGFSGSYLFGKLTRASSVVFPDSLGFINTKVDNQVKLGDLYY
                     DFGVQYQKMITSKMTLGIGGLYAPAQKLSANHNYFVRSFFTTSMGIESFRDTIVYITD
                     KGIVEIPEKYGVGIVLRNGVKWMVGADVDMQNWSKFKAFDVQDSLVNSIQIAIGGEYF
                     PSDKSIDGYWKKIKYRAGFRYNKSHLFLQNTQIKDFGISFGFGFPLPRSLTTINLGFE
                     IGRFGTKVNDLYQNNYFRVNLGVSVWERWFIKRKYY"
     gene            complement(19054..19818)
                     /locus_tag="PK345_03905"
     CDS             complement(19054..19818)
                     /locus_tag="PK345_03905"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR00671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HPX34199.1"
                     /translation="MNRFCSNNNKNDQRLIIDFGNTLTKAAIFVNDKIVDLLKTSELT
                     VNQLKTFTAERDVKYVFSSAVIDVSDDVNVFLKNSFQYLTFDHHTLLPIINLYKTPAT
                     LGKDRLSMAVAAADQFPEQNCLIVSAGTTITYDFVDSEKRYHGGAISPGLTMRLKALH
                     TYTSKLPLISLKADVELTGNTTENSILSGVIWGAIAEVDKIIDYYKASYTDLLVIFSG
                     GDVNIFHKKLKNCIFVAENLVLTGLNAILNFNVNRQ"
BASE COUNT         6474 a         3727 c         3789 g         6013 t
ORIGIN      
        1 aaaaactaaa actgacgaaa ataaaactga cgaagagata ttttgataaa gatttaggta
       61 aaatattttg atgctaaatt aatatttttc tgttagaaaa aattaccgta aatccttata
      121 attcacctgt ttgaccctgt cagattaata ttaaatatcc acttttcagg caactgttct
      181 cggcaaaaaa ccctctttgc cggtagattt atttttaaga gttttatttt gcacaattct
      241 tgatcattgg actgatcaaa attccaaata ttatgaagtt attgaaactg gcgtttttta
      301 ttgccatttt atcagcaatt gttctattca tcacagcaaa cattccacct atggacaaaa
      361 attatgatga taaatggaaa caggttgact cgttggtcag tttaaatcaa cctcgttcgg
      421 cgcttgaatt agttaatgag atatacaaaa tagcacaaaa cgaacagaac agcccgcaac
      481 tgatcaaagc aaatatctac cgaataaagc ttatggccga ttatgaggaa gattaccttg
      541 tgaaggcaat cagtgaaata aaaactgaaa taaaatcagc aggatttcct gagaagcagg
      601 ttttacattc acttcttggg gatctctatt tacgttattt tcatactaac cgttaccaga
      661 ttctcgaaag aagtaaggtg gcaggagaga ccagcgacga tattcaaacc tgggatgccg
      721 ctaaattact ttccactgcc gctagtcatt ttgaggtatc attatctccg gctaatcaat
      781 tacaaggagt ggacctgaaa gctttttcgg caatcttatt agaagaaaaa gactcaaaaa
      841 tatttagacc tacactatac gacttccttg cacacagagc tattgatttc tatgcttcgg
      901 gcgaaacagg aataacaaca gcagccgacc agtggaaacc cgatcatctt aattacttca
      961 actcagtaga gagttttata aacctgcaac ttcctgaaaa tatcgctgga acgttttcag
     1021 gaaaaatatt ggcaatttat caggagcttt tactgtttca tctgaacgac aaggacgtac
     1081 aagcgctcat tgatgttgac cttaaacgcc ttaactatgt gcactcaaac acaataatta
     1141 ccgaaaaaga tgggctttac caaaatgcgc taaacgcttt gctgaagaaa ttcaacaact
     1201 ctccctactc taccgcaata gcatttgagc tggccatgtt tcataatacc aaggcttcgg
     1261 aatataatcc caacatagct gatgattatc ggtgggaaag gaaaaaagca tctgatattt
     1321 gtcaggaggc gatcaagaaa ttccctaaca ctacaggagc ggcaaactgc cttatcttgc
     1381 ttgacgatat gataaaacca tcgctttcaa tacaaactga aatagcaact acggccgaaa
     1441 aaccctctct tggattaata aaatggaaaa actataacaa agtatggttc aggttgattg
     1501 ccatagatta caaagaatat caagagttat cgtggagagt gccgattgca gaccttgcca
     1561 ccaaactcat taaattgcct gctctcgaaa actggagtat agacctccct gatgaatctg
     1621 atctacaaca gcatacgacc gaattcaaaa taccggcgct gcaaaatgga ttttacatac
     1681 tgcttgcctc cggcaacaag gacttctcag gaaaagatca tcaacttgca tggacaaatc
     1741 tttggatcac aaatttaact ttcatacata agaaacttga atcaggaggt gttgaatttt
     1801 ttgttctcga ccgcataaag ggcacaccaa aatcaggagt taaatataaa gcttatgcac
     1861 gggtttatga ctttaaaaac cgtgaatata atgataagct ccttgcttca ggaacctctg
     1921 atcgaaatgg atacatctct atcctgccat cgcaacaatc cgccaataca tcaattacta
     1981 ttgagctaag tgaaaagaaa gacagatttt ttccggaaac ctctttttat cttggaccta
     2041 ccataaagac tgatccaaag cctgaaataa aaacctactt ctttacagat cgagctatat
     2101 atcgtcccgg ccaaacagta tattttaaag gtataatcat agaaaaaaca ggcgatgagc
     2161 aaaaggtgaa agcaaattat gagactaaag ttgatttttt tgatgttaat ttccaactga
     2221 tctcatccct cactctgact acaaatgaat ttggatcggt aaatggcgct ttcattatcc
     2281 ctaccggagt tctcaatgga atgatgacaa tacgtaatag ctcaggaggt actactttta
     2341 atgtagaaga ttataaacga cccacgtttg aagtagattt tctaccgatt caggataatt
     2401 ttaagttaaa caccgaaatc tctgtcgagg gcgaggtgaa ggcttttgca ggctatatga
     2461 taggtggcgc caatgtaaaa tacaacgttg tgcgcaggaa catgttccct taccgatggc
     2521 tgtcttacat ggatttcttc ccacgatcgt cggaaacaga aataacaacc ggtgaaacac
     2581 aagctgatga aaacggcaag ttttatatta agtttccagc aatccctgat cagcaaataa
     2641 acaggcgctt caagccggta ttctcctata cggtaaatgt tacagctacc gacattaacg
     2701 gcgaaacgca atcaggtagc cagatgatca tagtaggcta cgaagcgctt ttattggata
     2761 tagatatccc tgaaaaactc gatcgcaatc aggtgaaatc ttttaatttg gaaacggaaa
     2821 atttagctgg cgtaagacaa aaaaccattg ttagcgttga agtatcaaga ttaattgatc
     2881 ctggaaaact tctaaaagaa agactctggg aacaacccga tcaatttatt attccatcgg
     2941 atcaatttat taaagagttc cctgaacata cgtttaaaga tgaattaagt catgataatt
     3001 gggctgttgc tgaaacggta tttagtaaga atattaatac ggcaacggat agcttattga
     3061 ggttcgataa tctgtcgaac tggaaatcgg gtaagtatgt tgttgtttta aaggcaaccg
     3121 acaatttcgg cactcaggta gaaaccaaaa aatattttga ccttttcagc ctcactgacc
     3181 gccagccacc cgttacagtt tacaattggt tcgtaccatt gaaatcatcc ggcgaaccgg
     3241 gcgaagaagc tacatttctt attggttcgg cagcacagag agtaaaagcg ttatatgagg
     3301 tgcagcatca tggcaggact attagccgtc aatggatttc gttagagaaa gagcaaaaag
     3361 ttataacagt acctattaaa gaggattata gaggaggatt tacaatacaa ttaacttttg
     3421 ttgttaacaa ccgcgaattt aataacagga tgtgggtaac cgtaccgttt accaataagc
     3481 agctcgatct tacttttgaa acgtttcgca acgtattgga accaggtggg aaggaagaat
     3541 ggagagtaac ggtaaaggac aaaaagggtg atcaggctgc agcgcaaatg cttgcatcta
     3601 tgtttgatgc ttctttagag gcttttgtgc ctcacaactg gaactttaat ctttacatac
     3661 ctaattactt aagtaaccct tggcagttat ccggaagtat cggaacctcg ggaggaaata
     3721 actgggattt cactttacgt tctcataagt ctttccactt caatacatac gatcgcctta
     3781 actggtttgg ctttaacggc tttaggataa gtgattcatt tttttataaa agcggtgcaa
     3841 tgtcaaggga catggtacaa atgaatactc aagaattggc tgtcgtagag gaaagtactc
     3901 ttatagttga tgcatacgat gacggtaaac ttgcagaaat ggaaactgcc gaagagaaag
     3961 ctgaaaaagc taagcggata gagccttcat tattaaggcg ggactttaac gaaactgcat
     4021 ttttttaccc tgaattgcag acaaacgata aaggagaagt tatcattaaa ttttctctac
     4081 ccgaatcctt taccaaatgg aaattcatgg cattagcaaa taccagggat ctgatgacag
     4141 gtatgatgga gcaggaattc acagcaacaa aaaaattgat ggtaatgcca aacgtgccac
     4201 gcttcctgcg cacgggcgac actctgcaat ttactgcaag aattactaac ctctccgaag
     4261 aattgttaac gggaaatgca agccttgagt tttttgatcc gataacagaa aaaaatatta
     4321 ccgaaatatt aaaaacaaaa acaaaacaag gaacattctc tgtagcagcc ggtaaaacaa
     4381 catcggtaag ctggaatatc actgttcccg aagattttgg catcatcagt tatcgtataa
     4441 aagctactgc aggcaacttt tctgacggcg aggaaaaatc tgttccggta gtgccgagta
     4501 gaattttagt tactgaatca ttacccttgc caattaccgg cgcaagcagt aaagcgtttg
     4561 aattcaacaa attggttgag tcgggcaaga atactacaat caaacatcaa agcttgactt
     4621 tggaattttc gaccaacccc gcatggtacg ctattcaagc gctgccggtg atctccgaac
     4681 cgattcataa aaattcacac tccctctttg ctgcatttta tgcaaacagc atagcatatt
     4741 atatttcaaa tgcaaacccg aaaatcaaaa atgtttttga aagctggaaa agccaaactc
     4801 ctgaagcatt tctttcaaac ctcgaaaaga atcaggattt gaaagccatt atcttagaac
     4861 agacgccatg gctattgcag gcacgaaatg agagtgaacg taaacaaagg atagcgctac
     4921 tcttcgactt aaacaatatg cgcgaccgac ttgacgcatc cctgaaagcc ttgatacaaa
     4981 ttcaatcacc aaatggcgga ttcccatggt ttgaaggaat gcccgacagt aggtatatca
     5041 ctcaaaacat tgttgaagga ttgggcaaat tgcaaagtgt aagaatttta agtgcggtag
     5101 cggatgaaag agtggaacaa atgattacaa aagctatccg atatttagaa gagagaaaca
     5161 aggaagatta tcagtatatt ttgagtagga gaaaagataa aatcgaagaa gataatctgt
     5221 cagatattca tattcagtat ttatatgcca gaagcttttt cccacatatc aagatgaatc
     5281 cgagctgcga aaccgcattc aactatttta aaaaccaatc cgaaaagtat tgggcgaaac
     5341 gaaatatata tctccaaggc atgatcgcat tgacgttaca acgctacggt aataataata
     5401 taccaccttt aattgtcaag tcgttaaaag aaagatcatt gaaaaatgag gagatgggca
     5461 tgtactggcg acacgaaagc ggttattatt ggtatcaggc gcctttagag tctcaggcaa
     5521 tgatgattgc tgtttttgat gaagtggcag atgataaaga ggctgtagaa atgatgaaaa
     5581 tctggctctt aaaacaaaaa caaacacagg attggaagtc aacacgggct accgttgacg
     5641 ctatatatgc actgatgaaa aaaggtaaag atttattgac agaacctgaa aaagttaaaa
     5701 ttgaagtgaa tggcaaagaa ttgctaatga atgaagtagg taaaagagaa gcaggaacag
     5761 gatattttca aaaaacgtgg aactctccgg aaataatacc acaaatgggc aatataaaaa
     5821 ttacaaaaac cgatgatgga atagcctggg gagcaatgta ttggcagtat tttgaaaacc
     5881 ttgataagat cgcgccacat aaaacccctc tttcgattac aaaagagcta tttgttgaaa
     5941 aaagaggaac gaaaggtact gttattgaaa aattgaatga tgaagcaaaa ttaaagattg
     6001 gtgataaggt gattgtacga atcgaattgc gtgtggacag gaatatggaa tatgttcaca
     6061 tgcaggatat gcgtgcatcg gcgcttgaac cggtaaatgt tctttcaagc tataaatatc
     6121 gtggaggtct gggatactac gaaagcacaa gagatgctgc atcacatttc tttttcgact
     6181 tcctaccaaa aggaacatac ctatttgaat atcctctgat tgcctcacaa attggaagtt
     6241 tctccaacgg aataaccact attcaatgta tgtacgcacc tgaatttact tcacattccg
     6301 aaggaataag gattgtggtg gaatagatta ccataaaaag taacatgtga tgagccttat
     6361 ttttcaaaac acttagtttt gccgaaaata agattagatg gaatccattt gtgtattttg
     6421 cggctcaagc gttggtaaaa ggcctgagta tagcgagact gcgagaagtt ttggaaagct
     6481 aatagccagc cgtgatatca cccttgtata tggtggtggc aatatcggat tgatgcgcga
     6541 agtggctgat ggcgctttgg aagccggcgg taaagtaata ggcgtaatgc ccaaattgat
     6601 cgttgacaag gaaatagcgc acaacagcct tcatgaattg aaaattgtaa ataccatgca
     6661 tgagcgcaaa gctttgatgg cgaagctatc ggatggattt gttgccttgc ctggcggctt
     6721 cggcaccatt gacgagctat ttgaaattat gacatggaat cagcttggga taattaacaa
     6781 accttgtgga ttgttaaata ttgagggtta cttcaaccat cttttagagt atatatcttg
     6841 cgccgttaat gaacgcttcg tcagacctga gcatcaggca aacctcattg ttgacaatga
     6901 ccccgaagaa ttaattgacc gattggaaaa gtatagaccg caggttgccg aaaaatggat
     6961 tgaacgctta aaagttgacc ttatctaaaa ctgccctatg atagttattg gaattaccgg
     7021 aactcttgga gctggcaaag gaaccatagt tgattattta gttaaaaata aagggtttaa
     7081 ccatttttct gttcgtagtt ttattgctca agaaattcaa aatagaggaa tgacggttaa
     7141 ccgcgatagc cttaccgagg ttgccaacga tttgagatca gcaaattcgc catcctatat
     7201 cgttgatgca ctctattccc aagctttaag aattggcaaa aacagcatca ttgaaagtat
     7261 aagaacacaa ggtgaagtag agagtcttag agcaaacggc aatttttacc tttttgcagt
     7321 agatgcacct gccgaaatta ggtttaaacg ggttaaactt cgtggatcag aaaccgacaa
     7381 tattgatttt caaacattct tgcaaaatga aaaacgcgaa atgacaacta ttgatccaaa
     7441 caaacaaaat ctgaaaaaat gcattgaaat ggcagatttt atttttgaaa attcaggaac
     7501 catagctcaa cttgaagaaa gggttaaaaa ggtcattgaa aaacttttat aaataagtca
     7561 acttttaaaa gtaattttag ccgcaaatac ttcaactttg atcagtaacc atacacgaat
     7621 attaaatata taaacaactg aaaatctttg ttttcggagc ggacttttaa aacgaatcat
     7681 tatggaacaa tcttcatcgt cgaaaggtta tcagcgtcca acatgggacg aatattttat
     7741 ggagctggct aataccgctg ctaaacgcgc cacttgtgac cgaggtagaa gtggatgtgt
     7801 tatcgttcgc gacaagcatg tgttggttac aggatatgtt ggctctccgc ttggcttacc
     7861 gcattgcgat gacgttggac atctgttgaa aaaagtatat catgaagatg gccggataac
     7921 acaacattgc gtaagaacag tacatgccga gcaaaacgca atctgccagg cagcaaggtt
     7981 ggggatagcg cttgatggtg gcacattata ttgtaaaatg acaccttgcc acacctgcgc
     8041 aatgctgatt atcaattgtg gaataaaaag ggttgtatgt gaacgaaaat atcatgcggg
     8101 cgaagagtca gagaggatgt tccgccaagc tggagttata atagattttt ttaatgatga
     8161 agtggagcaa tacgataatc aataaactcc cctcctatgc atctttttga aaactttttt
     8221 atttgaattt cgctaaatgc tgccaggcgg caaataatat tcagcgctca gccccgaatc
     8281 aactctcctg atttcatata tcgtcagatt ggttttagga tccgaataga taatttcaac
     8341 atctgctttt tctgtctcct caaaaacatc ttctatttcc ttaaaaactg cttcgttgtt
     8401 gtgtttcacc ataacggtaa ttttggaaaa ggctaccaat agctcagatg cagtaaaagg
     8461 tttatcaagc cagttgaaaa acttgccttc tgattctttg aataaacaag tgttaggata
     8521 gagagtattg agttgctcac catacttttt aacacctctt ggcgtcatta acatcccgaa
     8581 tatcaggccg gcctcttttt tgaaatgcca attgtcggcg cttaaaatga gtacatcaga
     8641 ctgtggtaac ttttcaattt ctgttacaat ctcttttttg tagctcagat tctgccttct
     8701 tatttcaaga tatttcttat gctgctttat acttttcgga ttcgacaaca tcattaatgc
     8761 taccaagctg taaatgattg cttcgagcca ccatttcctg aattgaacaa tctcaattaa
     8821 aacattgatc gagaggtaac atataaatac tgtcaataag gaataaggaa caagataatg
     8881 ccctgcgtac tgctttgaaa tgattaaaat accgactatt aatgttgctg tgaaacctgc
     8941 cagcgctgct atatatcgtt tgttcccaat cctgcgtcgc acccagggca gcaaagaaat
     9001 gataaggacc aaaagagcta atatagtaat atatacaagg tatttttccg actgaataat
     9061 ggccttcaat tgtataaaaa actgatcagg atcaattacg gaggcctttc cgctcccata
     9121 ccttccgcta tttatagcaa gcgcctttgt ccatctaaaa aattccctgc tccttttaaa
     9181 tattgggaaa gtgaaaatta aaaaagagag agctgttgta cccaaatagt aactcttttt
     9241 tctaatccca gttagtaaaa ataaaggtgc aagaaaaaat ggtaaaaagg taagttttaa
     9301 tgagattcca aaaccaataa ttactccata aacaagccaa ctttgatatt tcagttttgt
     9361 attattgaag tacccataaa gatccaatac cgttatacta cacaaaacaa taacatgagc
     9421 aataaaccaa ggctcaggca taaaccgttc aatgatggaa ataacatgtt caaccacaaa
     9481 tggcgaaaac tgaataaaaa tagcagcagg aaggttgccg gttttacgat aaatacacca
     9541 cccagcgaaa aatattatca gggtaatgaa aacaatcatg ctgatattta gtgcattaag
     9601 ataaaaatca gggttttgta atacatcctg cgtaaacgaa gatgagggac gaaataaatg
     9661 aataatcctc attattattg ccgcaaatac tatcactgga gtgcctggtt gatcaataaa
     9721 cagattaata ctaccatgcc ctaacgcaat gccactgaac agtaaaataa attccggatc
     9781 aatattaaac atcgaatagt tttgattggc aagcctcaaa ttgataccga taaaaattaa
     9841 aaccaccgga ataacggcaa gcaacaatat gctgaaaagc tgcttaaata atatttttga
     9901 atgcacggct ttctaaaatt taaaagtaca agatgtttat ttagcttttt ttgtcaaaac
     9961 taaaagtttt atcttatttc aataataaac ttaaacccaa gtttatcatt agaaaatctt
    10021 tttgagttgt aaaattgatt ttcttttagc aatccatatt tagtattgtt cggtgcaatt
    10081 ttcattgtgg ttgttatatg tttttaatct gagttaatgc tctaattaat ttgataataa
    10141 ctacaaagcc actcatttaa cgtcttcaaa ttaaaatggc tttaaaatac tatttttggc
    10201 gctttaatta atgcacacat tatttatgtt gacagaggaa aaaaatcagg aaaatttaga
    10261 aacgcgatcg tccgtaaatt ttattgaggc tataataaac gaagacctga gattaggaaa
    10321 gaacaatagc agggtacata cccgttttcc gccagagccg aatggttact tacatattgg
    10381 tcatgctaag tcaatattgc ttaattacgg tattgcttgc aaatacaacg gaaaattcaa
    10441 cctcagattt gatgatacca atcccattaa agaagagcag gaatatgttg actcaatcat
    10501 ggaagatgtc cgctggcttg gcgctgattg ggaagaccgt ctcttttatg cttccgacta
    10561 ctttgatcag ttgtacgact gggcagagaa gatgattttg gatggtaagg cttatgttga
    10621 cgaccagtct gctgaagtga ttgccgaaca gcgtggggtg ccaaccgcac caggtatcga
    10681 aagcccatat cgaaatcgct taccggaaga aaaccttgac ctgttcagga ggatgaaagc
    10741 cggcgagttt caaaatggag aaagagtatt aagggcaaag attgatatgg catcgccaaa
    10801 catgcacatg cgcgatcctg tgatgtatcg tatcctacat gcatatcatc accgcaccgg
    10861 caaccaatgg tgtatttatc cgatgtacga ttgggcacat ggcgagtccg actaccttga
    10921 aggcatcact cactcaatct gcacgcttga atttgaagtc catcgccctc tgtacgactg
    10981 gtttgtggat cagatacagg aaactgatta tcgcccgcga caaattgagt ttgcccgcct
    11041 taacctctct tataccatta tgagtaaacg caaactcctt gagttagtaa aagagggtta
    11101 tgtgaatggc tgggacgatc cccgtatgcc tacaatatcc ggcttacgaa gaagaggata
    11161 tacgccggag gctataaaag tttttgccga aaaagtagga gtggcaaaac gagataatgt
    11221 cattgatgtc agtttgttgg aatttagcat ccgcgaaaac ctgaataaaa ctgccaaaag
    11281 ggtaatggca gtactaaatc cggtaaaact tactattact aactacccac aagatttaac
    11341 agaagacctt attaccgaga ataatcccga agacccggac gcaggtatgc gtaccattcc
    11401 attttcaaaa gaattattca tcgaacgcga tgatttcatg atttcgcctc cgaaaaaata
    11461 ttttcgactc tcccccggta aggaagtcag attaaaaagc gcatacatca taaaatgcaa
    11521 tgattatcgg ctggatgaaa gaggagaagt tgtagaaatt ttctgcactt acgaccctga
    11581 gagcaaaagc ggtggcgcgg gcagcaaccg taaggtgaaa ggcacattgc actgggttac
    11641 agtagctcat gctattgatg ctgaagttag attattcgac aggcttttca aagtggagaa
    11701 ccccgaagat gtggctgaag gagaagacta taaaacgaat attaatcccg attcattaaa
    11761 agttctgaac cagtgtaaat tggaacctat tatggcaaat gccatacctg gcgaaaaatt
    11821 tcagtttcag cgaattggct atttcactgt tgatcctgat acaacgcctg acaaacttat
    11881 atttaacaga acagttccat taaaagatag ctggtcgaaa agcgccttgt aggtaactaa
    11941 tatctcgtta aggctgagtt gatcaacagg aatttctttt agccagaacc attgattcta
    12001 taaggctaat cacgatttga atgttgtcaa ttgaggtggg tttatttata aagttgacta
    12061 accggtagag tttatcgcca aatacgatat ttacaagtcg ttgtaatacc acaaaatcaa
    12121 aaaaaaaaga cataaagtaa taagcctcgt cggtttactt tatgtctttt ctctattaaa
    12181 agatttatag ttctatctgc ctaacacaga aaaattatga attgttttaa taaaacatta
    12241 atctgttatc ctcaattttt gattcttttt ccaatgctct gataaaggca tttgagttta
    12301 ccctcgattc aaaggagttg cgtaattgga aaatagttgt accaaaatca tttacttctg
    12361 gcgcaggggt caaacgatca atcatagcaa cgaaaactgc attataaccg gctataggtt
    12421 tggatatctg tttcggctca acagtaaaca tctgtccaat aacatttggt tcgctaccaa
    12481 agccagggat atttcttgca gagaatgata gattttttgc agtatcaact tttgaattca
    12541 tattcgaagc aagatcgttt aaggtagttg cattaaagtc attgattttt ttcaccaaat
    12601 attcaccttt taatttgttg actaccaacg gccttatttg ttcttttact tgctcgaacg
    12661 gtgaatagcc tttatttttt atctctttaa gcgccgcgat aacgaattgt ttatcgtctt
    12721 caaaaattgt agagacgtcg ccaacttttg tagtttccca aaatgcccaa cgtatgattt
    12781 gcctgctttg ctccactccc gggattctat ttgatgtgct gcttatccgt tcggcagttc
    12841 ttttgttcaa cccctgatta acaaccgcag tatcgaattt ggcaagtgtg cggttttcgc
    12901 tggcaaattt gttggcttgc gaaaagacct gacgataggt ttggctgctg ggttcaacag
    12961 tacgcgttat ttgccctatt ttcaccttag ggataagtgc tgtcttccct gtaacaagta
    13021 atacatgaat tcctaatccg gtttccatag tatctggctc atttactttt aatgaaaccc
    13081 cctctttgaa aaaattggta aagcttctgt cgccatcagt catccagccc aatgcgcctt
    13141 tatcttctgc cacagaagga taatctgaat atttcaaagc caattcctca aatatagagg
    13201 gcgctttttt caaagccgta agaatactat ccaccaatac tttggcctct tcttccgaac
    13261 gttcgttgag ctggaagcct gcccttgcgg tgctatacga aaccagcatt tggctcattt
    13321 ctattgaatc gggacgttgc tgaaaatcca aaactttgat gatatggtgt gtatcacctt
    13381 cgatatatgg agggaataca gtatcaatct ttgcgctcat aattgattca gctaatgata
    13441 tcggcaagtc tgattctttt ttatatgtgc tatcgaatct tgtatccgaa acggaattaa
    13501 tgaaggcggc aggatcttcg gcatctttaa actgaacata tagtcggtca acttcttctt
    13561 tcacatcttg cctatcttcc tgagtcggta aaatttcaaa aaggacgtag tcaaaggttc
    13621 tcgaatcttc ctgtcggtag ttatatatat ggtcgttata atagtttctc aagtcattat
    13681 ccgttacatt gataagagaa tctgcaacat cagaatattt agctgcaacg tatatcacat
    13741 cagcgttttg attttttatt tggtaatcta attttgcgaa aggttgtggt atatggtaat
    13801 ttttcgataa caaatttttc attttggttc tgataatatc gtcctttacc attcgctcaa
    13861 ggcttagata tcttcttttc atttcagggc cttgctgatt aaggttcttc aagaaattgc
    13921 gaacaacttc cgggtcgaac gtttccgaat ttggatcgcg gaacgactgc tgaacataac
    13981 gatttgggtt atcgccaatt atttcgttcg aaagctcctc tttcgtaact atcagtccaa
    14041 tctttttata aacgtcgtta agtattgttt cttcaattat ctgattccaa acttgctgac
    14101 gaatttgaaa ttcctcttct gccggcactc tctcggtttg ttgctgtgct ctaaggttct
    14161 caagctgttc ggcaaccctg tcattgtaat tttgaatagg gatttccgtt ccatcaattt
    14221 taccgataat agtgattggt ctatttccgc ttggacggag aaaatcgccg agcacaaacg
    14281 ctgccaaagc aataccaact acgactacaa gtagtcctga atgttttctt atttttccta
    14341 taactgccat ttgatcaaga tttttttaaa gggtgcaaaa atacaataga taatgaaacc
    14401 tgaaaattta ttttacattg atttaagaaa cttattattc gctatcggta acgattttta
    14461 aattgattgt ttcaatccgg ttaatagcgg cttcgagtat gttgaattcc agattgttaa
    14521 ttacgatttt ttcatggagg tttgggatgc tttgatgatg aaagataata agcccgccta
    14581 aagtttcgta ctcttccgac tcaggaagat ttaaatcgta atggtcgttc aggtaatcaa
    14641 tttcaagacg tgctgaaaag atatattcat ggtcagagat ctttttttca atgagttgtt
    14701 caacatcaaa ttcgtcgtca atttcgccga aaatttcttc gatcacgtct tcaatagtaa
    14761 ctaagccgga tgttccgcca aattcatcta ccactaccgc aatgcttttg ttcccctgaa
    14821 taaatgtagt gagcgctgtg ctggcaagca ttgcttcagg aacaacaatg atggttcgca
    14881 gtattgattt gatagattta gggtttttaa aaagatcggc ggaataggta taaccgataa
    14941 tgttatcaat agaatcgcgg taaataggga tttttgaatg gccggattta ataaacgagt
    15001 cgcgcaattt attgatagga tcattttctt caagtgcgat aatctcagta cgaggcacca
    15061 tacactctct taactttaca tttcgaaaat ctattgcatt ttgaaacatt tggatttcct
    15121 gtgttgccgc ttcatttatc ttatctgctt ccgcagagta ttctttaagg taatggtcga
    15181 ggtctataag gctaaaaacc aaatggtcgt gcgaaaactt aatgttactg aatcgtttta
    15241 caataaattc cgaaaaccca atatatactt gaattacagg ataaagtaaa tagtaaagta
    15301 tagtaacagg tacggcaaaa aaagacaaga tctggtttgg attgattctg aaaagaacct
    15361 ttggcaagaa ctcggcaaca attaaaacta tgatggttgc aataagagtt tgagatagca
    15421 atatcgaaaa atcgctgcga attattggag gcaaaatttt gtcaatcgga tctttaagta
    15481 cttgtgccat aagaatccca tatacaacaa gtacaacatt atttccgatt aacattgcgc
    15541 cgataaattt ggaagggata cgaataaatc tcgaaaggat acgtgccgaa aagcttccat
    15601 tatttttatc cacttctatc tttaatcggt ttgcactaat gaatgcgatt tccatccctg
    15661 aaaagaaagc cgaaaaaatt aaaactatta ttatgtaaat gtaagtgtac attattcggg
    15721 ttcgttgtcc tgtatagtaa atacaccatg aggattgcgc aatgtatagt ttgtaaggct
    15781 ttcatttgcg tcaaaaccat cgccatagag cacaccatca ggacttgttc gttttacgaa
    15841 aacgttagaa aagatttttt gttctttacg gttccagata atatgttctg tattaagtac
    15901 ttcttgttta tcataattat tgatcactac atcatctttt acttccataa tgttagtctc
    15961 ttcccagctt acgccataat tagcagtaag ggtagctttt acgttcatca ttgaatcata
    16021 aaaatagagt gtaagccctt cggggaattc aagatagtga tcctccttaa tgtagcgatt
    16081 aagtaatggt gaaacgattt tcaaaataat tttgcccgaa tcgctataaa cggcctctat
    16141 gttcttggct gtttccaatg ggatattctc ttctaatgta agcgatgtta tagttttcat
    16201 atcattttta caggagtagg ttgttactcc tatacaaata atcaaagcaa tagtctttgt
    16261 tagggttctg aggtaatatt tattaggttt ggcgctcaca ttagcataaa taaaacagga
    16321 gagttgctat ttgaaagctc ttaccttagt tttttcgttt atccaacatt ccacagtata
    16381 atcgtcgcct tcagtcaggt tataaaagaa gatggtttcg gaacttggaa actgctgcga
    16441 ataaatagct atcctacttt gagctatttc agctacttca gggtcaattt gtttggcacg
    16501 tacatatttg tcaacggcta cccagtaaac gacttttttt gtcagctcgt tatcgccaca
    16561 ttccgaagag cttgaagcat acatatcgcc aatagaaatt aatatgttac cattgttagg
    16621 ttttaattca agcgactgta aagcatatct acgggatgta gaatagttct ttaattgttg
    16681 atgaacctta gatattaaaa agaggatatc tgctttttga tcttcattgt tatcctcaat
    16741 tgacttctca agataaggta ggctttcact gtatttttct ctttttataa gcattttacc
    16801 aatcatcagg ttcgatgttg gcgatggatc aagtttattc aattgtattg aagcgtcaaa
    16861 aaatagttgt gtatcggtac atcccttatt gtcaagcatt ttagtaattt tccttagtag
    16921 attaatatct tctggatttt cgtcgaattt tggctgataa atgcttacta ggtcctcgca
    16981 agtagcataa ggttcgaaag ccacatcaat gcttcctttt atgttttgcc atgaagctac
    17041 tacatcagcg tcatctttat tagcttctat gttgtaatcc attatctggt taagctcgtc
    17101 atatacttct aatactgtta ttttttctat caggttttca tctgccattt gtgttaccga
    17161 gcgaaagtaa taagccaaca cgaacgattc cgattcattt ccttgcagtt caactgacct
    17221 tttgaatatc ggataggctt ctttaaaagc atcgggacgg tattttagca gatcagttcc
    17281 tttgcgccca aggatatagc cttcacgtcc aaaattttca atccgttgat cgtaaatcat
    17341 catgagcgtg tcaacatatt tgtctttttt atcttccgat ttttcatttc tcaagaaatg
    17401 ttcaatcatc accgggccat caatgtatgt atattgagaa gcagccgggc aattaaaaaa
    17461 cacccatctc catggaccaa tagcatcatt caccgttgag ctggtaaatt cactggccct
    17521 ccattgtttg taaaactccc gataaagtga aagattggtt acacatttta cactatcttc
    17581 accatacttt tgagggttgt ctggtaataa aatgactgtt gccggggtgt tagcgttagt
    17641 tcttaaacta ttttcggcat ttattaatga cgaaaaagcc aagagcagta taaggctaat
    17701 tgttacgatt ttgattaatt ctttcatttt aaatatgttt aatagatagt aacttgtttt
    17761 ttaaataaca tttataaaaa tggttaataa tattttcgtt tgataaacca gcgctcccaa
    17821 actgaaacac ccaggttcac tctaaaataa ttgttttgat aaagatcgtt aactttcgtt
    17881 ccgaaccttc ctatttcaaa acccaaattt attgttgtta aagaacgagg taacggaaaa
    17941 ccaaaaccaa aactaatacc aaaatcttta atttgggtat tttgcaaaaa taaatgggat
    18001 ttattatacc taaatccagc ccggtatttt atttttttcc agtatccatc aatagatttg
    18061 tcgcttggaa aatattcgcc gccaatggct atctgaatac tgtttactaa ggaatcctga
    18121 acatcgaaag ctttgaattt actccagttt tgcatatcca catcggcgcc taccatccat
    18181 ttaacaccat ttctcaaaac aattccaact ccgtattttt cgggaatttc aacaatgcct
    18241 ttatcagtga tgtaaactat cgtatcgcga aatgattcta tccccatcga ggttgtgaaa
    18301 aaggaacgaa caaaataatt gtgattggct gataatttct gcgcaggtgc atatagcccg
    18361 ccaataccta aagtcatctt acttgtaatc attttttggt attgcacccc aaaatcataa
    18421 tagagatcgc ccaatttcac ctgattatca accttagtat tgataaaacc taatgaatca
    18481 ggaaaaacaa cggaagaggc tcgtgttaat ttaccaaaaa ggtaagagcc actaaaacct
    18541 attgcaaggt tattatgaag tttcaatgac gctccgataa aaaatctgtt tacaccaccc
    18601 gttccttcaa atgcaaattc tgaatttcca atctcaggaa ctaattcggt attttttatt
    18661 ttataaccta aattgctata tggagtaagg ccaatgctta cttttatcct tccggataca
    18721 ggaaatccaa ataggagata accaagcgat gcataattgg aacttcctga taaggtttta
    18781 gtagagatac taccgaactt gctgcttaac ccgccttcaa aaacaaaaga gagcgtgtcg
    18841 aagttggcat tagaggctgg gttattaaga tttacatgaa acgggctgct caaagcattg
    18901 gaaatgcctc ccattgaaaa actataggtt gaatttgatg cggataactc acctatccca
    18961 aatcgtgaat aaggcgaatt gacatcaacc tgtgaataac ctgcaaggct aaaaatcaaa
    19021 aagattaata gagataattt tttaaaacct ttgctactgc ctgttaacat taaaatttaa
    19081 tattgcattt agtccggtaa gtaccaaatt ttcggcgaca aagatgcaat tttttaattt
    19141 tttgtgaaaa atattcacat caccaccaga aaaaattacc aacaagtcgg tataagaagc
    19201 tttataatag tcaattattt tgtccacttc agctattgca ccccaaatta cacctgataa
    19261 tattgagttc tcagtagtgt taccggtaag ttcaacatcg gcctttagac ttattaacgg
    19321 tagtttgctg gtataggtat gtaaggcctt taaacgcatt gtcaagccag gagagatagc
    19381 gccaccatga tatctttttt cagagtcaac aaaatcataa gtaatagtag taccggcgct
    19441 aacaataagg cagttttgtt ctggaaattg atcagcagca gctactgcca tggataatct
    19501 gtctttgcca agcgtagccg gcgttttata aaggttgata atcggaagta gggtgtgatg
    19561 atcaaatgta agatattgaa aactgttttt aagaaaaaca tttacatcat ccgaaacatc
    19621 aattactgcc gagctaaaaa catatttcac atctctttcg gcggtgaaag tcttcaattg
    19681 gttcactgtc agttcagatg tttttaacaa atcaactatt ttatcattta caaaaattgc
    19741 agccttggta agggtatttc caaagtcaat aataagccgc tgatcgtttt tattgttgtt
    19801 tgaacaaaat ctattcattt gcaatgaatt aatagaaaaa tgattacaaa agtaatttat
    19861 acttttttta aattgcagtt gagttaaaaa aattatctca aaattaaaaa atgaaattat
    19921 ctttgcaacc caattttcag acaaggtacc atagctcagt cggtagagca acggactgaa
    19981 aatccgtgtg tccctggttc gat
//
LOCUS       DAOOAI010000035        19519 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_3721_length_19519_cov_4.623716, whole genome shotgun sequence.
ACCESSION   DAOOAI010000035 DAOOAI010000000
VERSION     DAOOAI010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 19519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 19519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19519
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3721_length_19519_cov_4.623716"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1140
                     /gene="dnaB"
                     /locus_tag="PK345_03910"
     CDS             <1..1140
                     /gene="dnaB"
                     /locus_tag="PK345_03910"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HPX34200.1"
                     /translation="YEDTTDVFDLLDKAEQKLFSISENNLRRDHNDMKTLVIEAVKHI
                     ENARNSDGSYRGVPSGFTRLDRITAGWQDSDLIVLAARPGMGKTAFALTMAKSITAEL
                     NRPVAIFSLEMSSIQLATRLIASETRLSFDKLKKGELAEHEWQQLNARINNLLAAPLY
                     IDDTPALTIFELRAKARRMKQQYGIQLIIIDYIQLMQGGIEKGNREQEISNISRSLKS
                     LAKELDIPVIALSQLNRSVETRPGLKKPILSDLRESGAIEQDADMVLFIYRAEYYKID
                     RLEDGESSTGIAQLIIAKHRNGALDEIKLRFDAKYAHFSDFEDGTNMYNVDRIHGNVN
                     FETNAVILPSKMNDDITDDLPDQDLYNNDGDDEIAEDGFSVAPPY"
     gene            complement(1235..2536)
                     /locus_tag="PK345_03915"
     CDS             complement(1235..2536)
                     /locus_tag="PK345_03915"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPX34201.1"
                     /translation="MIRLSLFNYVIKRFILTLFYIFICYNSESQNIKALFIGNSYTAS
                     NNLLAIINQLALSVGDTLEHSSNTPGGYTFQQHCSNTTTINLIEEAKWDFVVLQEQSQ
                     LPSFPDNQVAIMVYPYAARLDSMIKFYNPCATTTFFMTWGRRDGDPEYCPVFPPVCTY
                     EGMDSLLQLRYTIMAENNHAAIAPVAKVWRKLRTDNPEIELYSHDGSHPSNSGSFAAA
                     CTFYSILFGKDPELTSYNFTLDPLEAATIKHTAKAIVYDSLDHWFRFEIRPIADFSSS
                     VTNATVIFTNHSTNADSYFWDFGDGNSSTLVSPVHTYSETGRYEVSLEAIDVSCGKSD
                     IDKQIIEIITTGIFNIKENNVPVFYPNPAGSSLIVKTDTKVRYLTIYDSFGREVICQI
                     AENGCTEIFVDVSTLHNGVYYLVLSGEDYITSRSKFVKYGS"
     gene            complement(2789..3085)
                     /locus_tag="PK345_03920"
     CDS             complement(2789..3085)
                     /locus_tag="PK345_03920"
                     /inference="COORDINATES: protein motif:HMM:NF020317.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HPX34202.1"
                     /translation="MQTYILFTKLSTDLSYELKNRKRQGRAWMEQVKAKCPEVKFLAH
                     YAILGEYDFIDIYEAPDPETAAKVSIISQEHGALHAKSMLAIPYKRFLELTDEI"
     gene            complement(3180..4022)
                     /locus_tag="PK345_03925"
     CDS             complement(3180..4022)
                     /locus_tag="PK345_03925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34203.1"
                     /translation="MLVDKTLDRYKRTKDNFFIITVNSIAYFIISFIVIYLLGQLLTA
                     VIALQYDFKSVIYYHRLAWAIDSSDWTVESVKLLFSLAPLLSFIVGVICLIIYVLLYD
                     ERTNLKQFFLWGFAHGMVWFFGATLAGALLDKGIGYVIMYMYFQDTERTILSLLSLTA
                     LLLTAAFTTRWFLFSGNAYFNQLNEHNRSFFTYSQIIVPAILGTLILILSKLPLITYY
                     ELFTLLTMLVFIIPILATHRSFPTFYFDEIPITIKADKKAIITASLMLVAFRVIFHFG
                     IMIG"
     gene            complement(4049..5314)
                     /locus_tag="PK345_03930"
     CDS             complement(4049..5314)
                     /locus_tag="PK345_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPX34204.1"
                     /translation="MDIYTKFGDMGPLGRFNRESFGYFMFPRLEGEIAPHMMFMGKKR
                     LIWSLNNYLGLANHPEVREADTKATEQWGLAYPMGARMMSGHSVHHERLERELAEYEK
                     KEDAYLLNYGYQGMVSIIETLVNRHDVIVYDSESHACIIDGCRLHFGKRFVFPHNNME
                     RFERELERAYKLTQQTGGGILVITEGVYGMSGDLGKLRDILALKKKYPFRLLVDDAHG
                     FGTMGPTGAGTSEHLKVMDEVDLYFGTFAKAMAGIGAFVAGEKEAIEYLRYNLRSQIF
                     AKSLPMPMVFGALKRLELIRTRPELRENLWKIVNALQSGLKEKGFNIGDTESPVTPVF
                     LEGDIPEATHITWDLRENYNVFCSIVTYPVVPKGVILLRLIPTAVHTLEDVEYTIDAF
                     SKIKEKLDAGLYPKDKMVDAEQFMKAYTK"
     gene            complement(5483..8770)
                     /locus_tag="PK345_03935"
     CDS             complement(5483..8770)
                     /locus_tag="PK345_03935"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HPX34205.1"
                     /translation="MVNTFSPPSLEQLLKYILKDLNSKSEIFGISEKLFYKPWFYHVL
                     YTKSGGKFLHNPLGLASGPHTQLAQNIVAGWLCGARFIELKTVNETNYTKQNNPSIDV
                     PFEGYNCGSSHELPVREVFDQYLNAWILIHILHNRLYKSVRKDVEVGTVFNMSLGSSL
                     IQVRSEEILWFIDKMMNCSEELENKIKTIKNIYPLVTSLKIPTNISNSVTLSVEQGSV
                     PREIEYVVKYLMMDKKLHTTLKLNPTLLGVKSIRALLNKNRGYDVHIPDSVFDDNLQY
                     VDLIDLISKLQKTADEMKLELNFKVSNALNCLKESRALPKDENLFISGAALHPVAVNL
                     AANLQTRFEGNLNLSFSGGVDYYNVANLIKCGFKTITVCTDLLKPGGYGRLAQYFDSI
                     YKEFVKVGARNIFEYTIKTSNEASILEASLEHLKDYAIYTLSDKRYQRDLSKNYNIKT
                     SKKLYPYDCIEAPCVSQCSVNQDVPSYLWFAAKHDINKSFSFIIQNNPFPSITGSVCD
                     HLCKTKCTRINYDEPVKIREVKRYVSEKIETNQQDQPITQKNEKKICVIGGGISGLSC
                     AWFLNQAGFAVDVFEADAYPGGMVVNSVPAFRLEDKSILRDIERIRKSGVNIITKTVV
                     NSKKFLQLRKKYDYVFISTGFQVASISIIDGTGCKNIFEAIDFLKIVKQNKKVELGQN
                     VAVFGGGNHAVDAARTAQHLLGANGVTSLVCQYTINDIKADKEEVAAMIEEGVNIVEM
                     VFPIQIIREDNHVKGLEYRVVEQYETNKNGHIKPLKISKTIHSLEFDTIIFSGSYSSN
                     INFVEPDQLKTKEGSLLTRMKNVYIGGGARLGETTLIKAIADGRNAALEIMKKENILQ
                     LPTPEKILTVEEIAELLLKKNKREIAVKQKDVPIEERNNFEAVIHPLTKTQAIYEAKR
                     CLQCDVICNVCVSVCPNNAFIGFEIKPEELLIPTINYNNDNFKVSKKKRLDIKQKYQV
                     ICVADWCNECGNCTTFCPTAGTPYKDKNRLHFDKAAFKNEGRGFLLSTDGDLKQLIMK
                     KDGKLSVLSLNWDALIFENDDCMAVLDKNTFEIEHIDIFTDSKRTFDLPEITEMKIIF
                     NAVENLV"
     gene            complement(9100..9600)
                     /locus_tag="PK345_03940"
     CDS             complement(9100..9600)
                     /locus_tag="PK345_03940"
                     /inference="COORDINATES: protein motif:HMM:NF027349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4783 domain-containing protein"
                     /protein_id="HPX34206.1"
                     /translation="MAKFGLHFCSVVLLFFALTLPAQEVSVKALEVKPNPDSTILLIA
                     DNFNEPALLPLQDSLLYAQILDAIAAANAYQLSEYFDVAVNLILPQNEGAYSKKQATQ
                     LLKYFFEKNPVKEFLLEYEDKTDKDLTYLIGFYTTTDNRVFRVFILIKKLNDTELIRQ
                     IQFEEK"
     gene            9761..10234
                     /locus_tag="PK345_03945"
     CDS             9761..10234
                     /locus_tag="PK345_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HPX34207.1"
                     /translation="MKIRLLMIGKTKERFIEEGYSFYRNRIDKYLSFTDHTIPSLKNT
                     RNFTPEDFKIQEGKLILKHCKENEYIVLLDEEGKQVDSLGFAGFIQEIMNSSIKTLTF
                     VIGGAYGFSDEVYRSAKMQLSLSNLTFSHQLARVIFMEQLYRSMTILNNEPYHNE"
     gene            10400..11233
                     /locus_tag="PK345_03950"
     CDS             10400..11233
                     /locus_tag="PK345_03950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPX34208.1"
                     /translation="MIKPKEHLTSLKNPKIQNMVSLHKSRESREKGVFLIEGVRELEK
                     AVKAGYHLTSLFYCPELISIEYLKIFSGKTTEVYTITKEVFQRITYRGNNGGLIATAK
                     RKEHHIIGLQLNKNPLLLILESVEKPGNLGAILRTADAAGVDAVIVCDQQTDLYNSNV
                     IRSSLGCIFTIPVFVGSSAEVIDLLKANEIKIYCTSLLASVPYYTIDFTISAAIIMGA
                     EATGLTPIWLDHSDQNIVIPMRGNVDSLNVSTSAAIVIFEAMKQRRFEKSKRAKSNLS
                     K"
     gene            complement(11253..11909)
                     /locus_tag="PK345_03955"
     CDS             complement(11253..11909)
                     /locus_tag="PK345_03955"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HPX34209.1"
                     /translation="MEHTLTKHYEAIIWDWNGTLLDDLEICIDAMNMMLHKRGYKLLE
                     KQHYKEIFTFPVRDYYERVGFDFEKHDWETIALEFIRNYRDLIHKSLLHDRVNEVVSY
                     FQTAGKRQFILSAMHQDFLVETISARLDPSKFEIIAGLRDHYAESKAGNAHILVDKTG
                     LSKDRIVMIGDTIHDYEVAHETGIDCILVADGHQSKERLETTGTKVLNNLGELIDLFN
                     "
     gene            11957..13321
                     /locus_tag="PK345_03960"
     CDS             11957..13321
                     /locus_tag="PK345_03960"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HPX34210.1"
                     /translation="MKIKSINPATGEINETYLLHTLPIVNDIINSVDLAQKEWVATSF
                     IERSMLMRNAAKLLRKQKPILAQLITHEMGKVIRESEAEVEKSALVCDYYADNAKAFL
                     SDEVINSDAKSSFVAFEPLGVILAVMPWNFPFWQVFRFAAPALMAGNAAVLKHASNVP
                     GCSVAIEKIFTDSGFPENLFRSLLIPASEVENIIGNKIIKAVILTGSESAGRHVAKAA
                     GKHLKKTVLELGGSDPFIVFKDADLNKCSEVAVMARMINTGQSCIAAKRFIVVEEIAD
                     EFIKLVSQKMSELQSEGPMDPRSDFGPLARPDLLIELQSQIDRSVKMGAKIELGGNPM
                     KRDGFYFEPTIMSKVTEDMPVFTEETFGPVMAIVVVENEEKAIELANKSNFGLGASIW
                     TQDRDKGIRLARKIEAGAVFINGLVKSDPRLPFGGIKNSGYGRELSNYGIKEFVNIKT
                     IWVG"
     gene            13435..14118
                     /gene="trmD"
                     /locus_tag="PK345_03965"
     CDS             13435..14118
                     /gene="trmD"
                     /locus_tag="PK345_03965"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HPX34211.1"
                     /translation="MRIDILTIFPQMFEGIFQHSILKRAISKDLASIFLHDIRDYSTN
                     KHRRVDDYAFGHGAGMVMMIQPIADLIEKLKSERNYDEVIYLTPDGIPFKQKITNRLS
                     TLTNIILLCGHYKGIDERLREHFITMELSIGDYVLSGGELAAAVVTDSIVRLIPGVLG
                     DETSALSDSFQDGLLSPPVYTRPAVYKGWEVPRILLSGDEKKIDEWLTEKAIERTRSR
                     RPDLLEEME"
     gene            14249..15130
                     /locus_tag="PK345_03970"
     CDS             14249..15130
                     /locus_tag="PK345_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34212.1"
                     /translation="MMVLVCGCASIKANPDSLFIKKIDSIALQLYSVQPDSIKLAANA
                     IILEHITAFLKTPQSIAFPFDSLKFVKVVTPSNSSFKLITWVFPLSDKNQYHYSGLIQ
                     HLKDNMPDRLTELRQSDDEPTPFLTYRYDQWPPAVYYQLIESKTKSGTLFTLFGWVGG
                     TQGEAARVIETLSFNENHEALFGVPAFKLNKETILNRVYFNYSNEVPFHLAYEQHYVP
                     GKKRKKAWMIVHNRLVNSDSNMGNAFRFSLPSYSEFDGFIFSDNCWQQVKDIDVRIKI
                     KSAKKKKQPKTGLFPND"
     gene            complement(15673..18255)
                     /locus_tag="PK345_03975"
     CDS             complement(15673..18255)
                     /locus_tag="PK345_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPX34213.1"
                     /translation="MKKYLLLTFITLLIFCIYAQTGNNVTTVDEIFGSKGEIYFKFEN
                     KEINLEALSKMISIDKVNWHDVFAYANKTEFGEFLKQGIDFELLPKPGELTKEIKMLD
                     NVNVKGILDWDFYPTYPAYVDMMYQFASEYPQLCQVFSIGQSIQGRELLMAKVSKNVQ
                     VREAEPQFLYTGTMHGDETTGYILLLRLIDYLLSNYGTNSKVTNLLDNSEIWINPLSN
                     PDGTYKGGNNSVYGATRYNANNVDLNRNYPDPEDGPHPDGNEWQPETVAFMQLAEEQH
                     FVMSANTHGGAEVLNYPWDTWSPLPADNTWWVFVCRQYVDTVHLYSPPNYMNQFNNGI
                     INGYSWYSISGGRQDYMNYFHNCREVTMEISNVKLLPTSQLENHWNWNYRSLLNYLEQ
                     SLYGVNGTVTNITTGQPINAKITIEGHDIHNSFVFSEQQTGFYQRLLDAGTYNLTFTA
                     PGYYPVVINNVNVTRYNTVNLNVEMDAGELEAGFSASETTIAIGDAINFTDESYGAPV
                     SWSWSFSGGIPSTSNMQHPQNITYPNAGSFPVSLTVANENGETNTITKEDYITVNATF
                     LMSNQTVTICNGIFYDSGGDTGNYSNNEDYLMTFKPETAGANIIVQFIYFDVEYNSTC
                     NWDWLKIYNGINTSASLIGTYCGTNSPGTIEASNDQGALTFQFHSDYSVNKSGWKAIL
                     SCSFVGLPPIADFSANQNLIYRGDTVMFTDLSTNNPTSWSWSFEGGTPPVSNLQNPSI
                     TYYELGEFDVKLIVQNDYGIDSLLIQNFIKVDSGIGIEKRYAGNVSVYPNPVKNDKIT
                     ITSDMMIKEVKVIDLFGSLIKYEKDINATNVRLDMSQIRSGVYLVEILDDFGCRSIKI
                     TVIK"
     gene            complement(18284..19168)
                     /gene="dapA"
                     /locus_tag="PK345_03980"
     CDS             complement(18284..19168)
                     /gene="dapA"
                     /locus_tag="PK345_03980"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HPX34214.1"
                     /translation="MKFNFTGTGVALVTPFHKNGNIDFCSLEKVVEHTIAGNVDYLVA
                     LGTTGETPTLSNDEKLAVVDFVIECAAGRVPVVVGVGGNNTQEIIDKIKTMSFEGIAG
                     ILSVSPYYNKPQQKGIYYHYKTVSSVCPVPMILYNVPGRTGANMSAETTLQLASECDN
                     VVAVKEASGNVNQIMEIIRCKPDNFHVISGDDAITLPLISLGANGVISVVANAFPSDF
                     SKMVNLCLKGKFAEANVIHYKLLPIMQKIFEDGSPAGIKAALQILEIAANNLRLPLVK
                     VNKAVNLQLQQLIAEYQN"
     gene            complement(19171..>19519)
                     /locus_tag="PK345_03985"
     CDS             complement(19171..>19519)
                     /locus_tag="PK345_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34215.1"
                     /translation="SIIAYLPMKQIPNYYLAKLKMDIITSKDLNLLGICKKPFVESFI
                     QKDFDLLIDFSAGDILPLNYISGYSHANFKTGCFTQKMVEVFDFMIKNTDGMESREFA
                     ETMVSYLRTINSK"
BASE COUNT         6188 a         3827 c         3503 g         6001 t
ORIGIN      
        1 tatgaagata ctaccgatgt ttttgattta cttgataagg ctgaacaaaa attgttttca
       61 ataagcgaaa acaatctccg ccgtgatcat aacgacatga agacgttggt gatagaggct
      121 gtaaaacaca ttgaaaatgc ccgaaactct gatggcagct atcgtggagt gccatctgga
      181 tttaccaggc ttgatcgtat cactgctggt tggcaggatt ccgaccttat cgtgcttgcc
      241 gccagaccag gtatgggaaa aacagccttt gctcttacta tggcaaaaag cataacagcc
      301 gaacttaatc gtccggtagc aatcttttcg cttgagatgt cttccattca attagctaca
      361 agacttattg ccagcgaaac cagactatcg tttgataaac taaaaaaagg agaattagca
      421 gaacacgaat ggcaacaact caatgcacgg ataaacaact tgttggccgc cccactctac
      481 attgacgata caccggcgtt aacaatattc gaactacggg ccaaagcccg ccgaatgaaa
      541 caacaatatg gcatacagct tatcataata gattatattc aactgatgca aggaggcatt
      601 gaaaaaggaa accgtgaaca agagatcagt aatatttcca gatcgctaaa aagcttggca
      661 aaagaactcg acatcccggt tattgccctg tcgcaattaa accgttcggt tgaaacacga
      721 ccaggactga aaaaaccaat cctatccgac ctacgcgaat caggcgctat tgagcaagat
      781 gccgatatgg tacttttcat ttatagagcc gaatattata aaattgacag gcttgaagat
      841 ggagagtctt ctacaggtat tgcacaactt atcatagcca aacaccgaaa cggagcatta
      901 gacgagataa aactcagatt tgatgctaaa tatgcacatt tctccgattt tgaagatgga
      961 acaaatatgt acaacgttga tagaatccat ggaaatgtaa acttcgaaac aaatgctgtt
     1021 attttaccct caaaaatgaa tgatgatata acggacgatt tacccgatca ggacctttat
     1081 aataatgatg gcgacgacga aattgcagaa gatggattct cggtcgcacc tccatattaa
     1141 tatcggatag ctgattagag caaaaaactt aaaaaaaaaa cagaatatca atcccactta
     1201 cacctttcgg gaaaacataa tgtgtagagc aaaactatga tccatatttt acaaactttg
     1261 atctactcgt aatataatct tcaccggaaa gaacaagata gtacactcca ttatgcaaag
     1321 tacttacatc aacaaagatt tcagtgcagc cattttcggc aatctggcaa ataacctctc
     1381 tcccaaacga atcatagatg gttaaatatc tcaccttagt atctgttttt acaattaggc
     1441 tgctgccggc aggattaggg taaaaaaccg gaacgttatt ttctttgata ttaaaaatcc
     1501 ctgtagtaat tatttcaatt atctgtttgt caatatctga cttgccgcat gaaacatcta
     1561 tagcttcaag gcttacctca taacgccccg tctccgaata tgtgtgaacc ggcgaaacta
     1621 atgtgctact atttccatca ccgaaatccc agaaatagct atcagcattt gttgaatgat
     1681 tagtaaatat aacggttgca ttagtaacag aactgctaaa atcagctatt ggtcgtattt
     1741 caaatctgaa ccaatgatcg aggctatcat aaacgatcgc tttggcagta tgtttgattg
     1801 ttgcggcctc aagcggatcc agtgtaaagt tataagatgt tagctcaggg tctttcccga
     1861 acaaaataga atagaaagtg caggctgctg caaatgatcc actattactg ggatggcttc
     1921 cgtcatggga atataactct atctcggggt tatcagttct gagctttctc cacactttag
     1981 ctaccggcgc tattgcagcg tggttatttt cggccattat agtatatctt aactggagaa
     2041 ggctatccat accttcataa gtacacacag gcggaaaaac cggacaatac tcagggtcgc
     2101 catctcttct tccccaggtc ataaaaaatg ttgttgttgc gcaaggattg taaaatttta
     2161 tcatgctgtc caacctagcc gcgtaaggat ataccatgat tgcaacctga ttatcgggaa
     2221 aggaaggtaa ttggctttgc tcctgaagta cgacaaaatc ccatttagcc tcttctatca
     2281 aattgatggt tgtggtgttt gaacagtgct gctggaaagt gtatcctccg ggggtattac
     2341 tactgtgttc caaagtgtcg ccaacagata atgctaattg attaataatt gcaagtaaat
     2401 tgtttgatgc agtatagctg ttacctataa ataaggcttt gatattttgc gattcggaat
     2461 tataacaaat gaagatgtaa aatagagtta ggataaatct ttttatcaca taattaaaaa
     2521 gggacaatct gatcataaca ctttaacttt taagattaag aagactacaa taagtaattt
     2581 tagccacgaa tattccaact ttgttgaaca accataaaca aacaataaat ttataaatca
     2641 ttgaaattct aaactttact caattactat ttaagttaat attttaaaaa actctatttt
     2701 cgtaacgggc tcaaaattaa gttaattcaa aaaaagccgg gggtaaaaaa gcatgcgctt
     2761 ctatcccggc ctttgtaatt tccgctgatc aaatctcatc cgttaattca aggaagcgct
     2821 tgtagggtat ggctaacatg ctttttgcat gtaaagcccc gtgttcctgg ctgattatgg
     2881 aaaccttagc agccgtttcg gggtcgggag cttcgtaaat atcaataaaa tcatactcac
     2941 caagaatagc ataatgcgcc aaaaacttta cctcaggaca ctttgctttt acttgttcca
     3001 tccatgcacg tccttgacgc ttgcggtttt ttaattcgta agagagatca gtcgagagtt
     3061 tggtaaacaa aatgtacgtt tgcataaata cctcctttgc aaatttttaa aatgtgattg
     3121 ccaaagtaca aaaaaaaatc atataatgac aatttcacaa ataaaaattt ctatcaaaat
     3181 taccctatca taattccaaa atggaaaata acccgaaatg ctacgagcat cagtgaagca
     3241 gtaataatag cttttttatc tgctttaatg gtaatgggga tttcatcaaa ataaaaagta
     3301 gggaaactgc gatgggtagc caatattgga ataataaaga ctaacatggt aagcaaggta
     3361 aaaagctcat aataggtaat taatggcaat ttcgatagta tcaggattag agtgcccaaa
     3421 atggcaggaa caattatttg actataagta aaaaaagaac gattatgctc attcaattga
     3481 ttgaagtatg catttcctga aaaaagaaac caccttgttg taaaggctgc tgtaagtaat
     3541 agcgcagtta gggatagaag tgataggata gtcctttcgg tatcttgaaa atacatatac
     3601 atgatcacgt atcctattcc cttatcgaga agcgcgccgg ccagcgtagc gccaaaaaac
     3661 cataccatgc catgagcaaa accccacaaa aagaactgct ttaaattggt acgttcgtca
     3721 tacagcagta catagattat aagacagata acgccaacaa taaacgaaag taaaggagct
     3781 aagctgaata acaacttcac cgattctacg gtccaatcac ttgagtcaat agcccaggca
     3841 agcctatgat aataaataac gcttttaaaa tcatactgta aagcaatgac agcagtgagc
     3901 aactgcccaa gcaaatagat gacaataaaa gagatgataa agtatgctat cgaattaaca
     3961 gttatgataa aaaaattatc ttttgtcctc ttatatctat caagtgtctt atcaaccaac
     4021 atgattcttt taactcattt acttcatatt atttggtata tgctttcata aactgttcgg
     4081 catctaccat cttatcctta ggataaagtc ctgcatctaa tttttcttta atcttcgaaa
     4141 aggcatcaat tgtatattca acatcttcga gggtatgtac agccgttggt attagcctga
     4201 gaagaatcac tcctttcggc actacgggat aagtaacaat agaacaaaaa acattatagt
     4261 tttcgcgcag atcccaagtt atatgtgttg cttcggggat atctccttcg aggaataccg
     4321 gagtaacggg cgattccgta tctcctatat taaacccttt ttctttcagt cccgattgta
     4381 gcgcattaac aatcttccaa agattttcac gtagttctgg acgtgttctg atcaattcca
     4441 aacgttttaa ggctccgaaa accataggca taggcaggga tttagcaaat atttgcgaac
     4501 gcaggttgta tctcaagtat tcgatagcct ctttttcgcc ggctacaaag gctcctatac
     4561 cagccattgc tttggcaaat gttccaaaat agagatccac ttcatccatt accttgagat
     4621 gctcggaggt tcctgctccg gttggtccca tagtaccaaa accatgggca tcgtccacaa
     4681 ggagcctgaa ggggtacttc ttttttaatg ctaatatatc acgaagttta cccagatcgc
     4741 ccgacatacc atagacaccc tctgtaatta ccaaaatacc gccaccggtt tgttgtgtga
     4801 gtttgtaagc tctttccagc tctctttcga acctttccat gttattatga ggaaaaacaa
     4861 aacgtttccc aaaatgaagc ctgcaaccat caattataca ggcatgagat tcggagtcat
     4921 aaacaataac atcgtggcga ttcactaaag tttcaattat tgataccatt ccctgatatc
     4981 cgtagtttag caggtaagca tcttcttttt tttcatattc agcaagttcg cgttccaacc
     5041 gttcatgatg caccgaatgc cccgacatca tacgtgcacc cattggataa gccagtcccc
     5101 attgttcagt agccttggta tcagcttcac gaacttcagg atgattggca agacctaagt
     5161 aattattcaa cgaccagatt agacgttttt tccccataaa catcatgtgt ggagctattt
     5221 caccctcaag gcgaggaaac ataaagtaac caaaactctc acggttaaac ctccctaacg
     5281 ggcccatgtc accaaacttt gtataaatat ccattttact aatttttaaa tttggctgca
     5341 aagctaagat ttattaaggg gatttttaat aataccactg aataaacact aaatcaggat
     5401 aaataaaagc aaaaattcag ttctaaaagg ctctatatta atcaattcgg gataataatc
     5461 aataatgatt actaaaatga agctaaacta aattttcgac agcattaaat attattttca
     5521 tttctgtaat ctcgggcaga tcaaaagttc ttttactgtc ggtaaaaata tcaatatgtt
     5581 ctatttcgaa agtgttttta tcaagaacgg ccatgcaatc gtcattttca aaaatcaacg
     5641 catcccaatt taaggagaga accgacaatt tcccatcttt tttcataatt agctgtttca
     5701 aatccccatc agttgataat aggaagcctc taccttcatt tttaaaagcg gctttatcga
     5761 aatgcaagcg gtttttatcc ttataaggtg tccctgccgt tgggcaaaaa gtagtgcagt
     5821 taccacattc attacaccag tcggcaacac atataacctg atatttttgt ttaatatcaa
     5881 gtctcttttt cttacttact ttaaagttgt cgttattata atttattgtt gggataagca
     5941 actcttcagg ttttatttca aaaccgataa aggcgttatt agggcaaacg ctgacgcaaa
     6001 cgttgcagat cacatcacat tgcaggcagc gcttggcttc gtatatagct tgagttttag
     6061 tcaatggatg gataaccgcc tcaaaattat tacgctcttc tattggtaca tccttttgct
     6121 ttactgcgat ttcgcgcttg ttttttttca acaacaactc agcaatctct tctactgtca
     6181 atattttttc gggcgtcgga agctgtagaa tattttcttt tttcataatc tccaaagcag
     6241 cattccttcc atcagcaata gccttgatca gcgtagtttc gccgagcctg gctccacctc
     6301 cgatatacac atttttcatg cgggtcaaca atgagccttc tttggtctta agctgatctg
     6361 gttcgacaaa attgatattt gatgagtagc taccactaaa aataatagta tcaaattcta
     6421 aactatgtat cgttttcgag atttttagtg gtttgatgtg tccgttttta ttagtttcat
     6481 attgttccac tacacgatat tcaagacctt taacatggtt gtcttctctt attatctgaa
     6541 taggaaaaac catttcaaca atattgactc cctcttctat catcgcagca acttcctctt
     6601 tatcggcctt tatgtcattt attgtatatt gacagactaa tgaggttact ccgttggctc
     6661 ccaacaaatg ctgagctgtg cgtgctgcat ctaccgcatg attcccccct ccaaatactg
     6721 caacattttg cccaagctca acctttttat tttgctttac aatctttaaa aaatctatag
     6781 cttcaaaaat gtttttacaa ccggtaccat caataataga gatacttgcc acctggaagc
     6841 cggtagagat aaaaacataa tcgtattttt ttcttagttg gagaaacttt ttactgttca
     6901 ctacagtttt tgtaataatg tttactccac tttttcttat acgttcgata tcccttaaaa
     6961 tacttttgtc ttctaagcga aatgctggaa cagaatttac caccattccc cccggatagg
     7021 catcagcttc aaaaacatct acagcaaaac cagcctgatt aagaaaccat gcacaagaga
     7081 gaccggatat accgcctccg ataacacaga tttttttctc atttttttga gtaataggtt
     7141 gatcttgctg attagtttct atcttttcag acacatacct cttgacctcc cttattttca
     7201 ccggttcatc ataatttatc ctcgtgcatt ttgttttaca taagtgatcg caaacagaac
     7261 cggttattga aggaaacgga ttgttttgta taataaaaga aaacgattta tttatatcat
     7321 gtttcgctgc aaaccacaaa tagctcggta catcctgatt gacagaacat tggctaacac
     7381 agggcgcctc gatacagtca taaggataga gctttttaga ggtttttatg ttatagtttt
     7441 tagataggtc tctttggtat cttttatcac taagcgtata tatagcataa tctttcaaat
     7501 gttcgaggct tgcctctaaa atgctcgctt cattacttgt cttaattgta tattcaaaaa
     7561 tattccgggc tcctactttt acaaattctt tatatatact atcaaagtat tgagctaagc
     7621 gaccatatcc tccaggtttt aaaagatcgg tacagacggt tattgtttta aacccgcatt
     7681 tgattaaatt agcaacatta taatagtcaa cacctccgga aaacgataga tttaaatttc
     7741 cttcaaatct tgtttgaagg ttagcagcaa ggtttactgc aacaggatgc aatgcagcgc
     7801 cacttataaa gaggttttca tcttttggta aagccctact ttcctttaaa caattaagag
     7861 cattggacac tttgaaatta agctccaatt tcatttcatc tgccgtcttt tgtaatttgg
     7921 aaatcaggtc aattaaatca acatattgca gattatcatc aaaaactgaa tcagggatat
     7981 gcacatcata tcctcgattt ttatttaata gcgcacgtat agacttgacg cccaaaaggg
     8041 taggatttaa tttaagtgta gtatgcagtt ttttatccat catcagatat ttaactacat
     8101 attcaatttc acgtggcaca ctcccctgct ctaccgaaag agtaacacta tttgaaatat
     8161 ttgtaggaat ttttaaagat gttaccaacg gataaatatt ctttatagtt tttattttgt
     8221 tttcaagttc ctcggagcaa ttcatcatct tatcaataaa ccatagtatc tcctcactcc
     8281 taacctgaat caaacttgac cccaaactca tgttaaacac agtacctacc tcgacatcct
     8341 ttctaactga tttgtacaaa cgattatgta aaatatgaat cagaatccat gcattaagat
     8401 attgatcaaa aacttctcta acagggagtt cgtgtgaact tccgcaatta taaccctcaa
     8461 agggcacatc aatacttgga ttgttttgtt tagtataatt ggtttcattt accgtcttca
     8521 attcaataaa tctggcgccg caaagccagc ctgctacaat attctgtgca agctgagtgt
     8581 gtgggccgga agcaagaccg agtggattat gaagaaattt tcctccagat ttagtatata
     8641 aaacatgata aaaccatggt ttataaaaca acttttcaga aataccgaat atctccgatt
     8701 tactattaag atcctttagg atatacttta aaagttgctc caaagatggt ggagaaaaag
     8761 tgttaaccat gatttttcaa aaaggtggat tttgagttgc aaatatactt attattataa
     8821 catgataaaa aaaaacacta ttaatgtttt caatttgtaa tggatttaat taactttaat
     8881 ttacaaagtt tgaatctttt aaaataatat attgattaat agggttttaa taaaaaaatt
     8941 ttattttttt gcctctatta ttgacagata aaatatttaa ttgctttcaa tcagcaaaac
     9001 gggaattata attaatggcc actaaatcgg gattacttcc ataaaaaaca tagcttcacc
     9061 caaaaaaatc tactgatcaa ttcaggttta aaaaccaact tatttttctt cgaattgaat
     9121 ttgtcgaata agctcggtat catttaattt tttaatcaaa ataaaaacac gaaacaccct
     9181 attatcagta gttgtataaa aaccaatcaa ataggtcagg tctttatctg ttttatcttc
     9241 gtattccaaa agaaattcct tgactggatt tttttcgaaa aaatatttta atagctgagt
     9301 tgcctgcttt ttactatagg cgccttcatt ttgcggcaaa ataaggttga cggcaacatc
     9361 aaaatattct gataactgat atgcgtttgc cgcagctata gcatccagaa tttgagcata
     9421 aagcaacgaa tcctgaagtg gtaataatgc aggttcgtta aaattatcgg ctattaacaa
     9481 tatcgtcgaa tcagggttgg gtttaacctc aagcgctttc acgctaactt cctgagcagg
     9541 aagcgttaaa gcaaaaaaaa gcagcacaac agaacaaaag tgtaatccaa atttcgccat
     9601 aaacatcact ttatgtaaat ataaattagt tttgccaatg atcgcaaatt tagtcatttt
     9661 agttttatgc agtatctttt tcctttttcg aaaaaaaaca gattgcatat aatgtgccaa
     9721 tgtcaaatag gttgaacaat attaaactac tcttttgtaa atgaaaatca gattattaat
     9781 gattggaaaa acgaaggaac gattcattga agaggggtat tcgttttatc gtaaccggat
     9841 tgataaatat ctctctttca ccgaccatac catcccttcg ttaaaaaata caagaaactt
     9901 tacgccggaa gactttaaaa tccaggaagg aaagctgata cttaagcatt gcaaggaaaa
     9961 tgagtacatc gtattattgg atgaagaagg caaacaggtt gattccttgg gctttgcagg
    10021 ttttattcag gaaataatga atagtagcat caaaacttta acatttgtta tcggtggcgc
    10081 ttatggcttt tcggatgaag tctatcgttc tgcgaagatg cagctatcgc tatccaacct
    10141 caccttttct caccaattgg ccagagttat ttttatggag cagctttatc ggtcaatgac
    10201 aattttaaac aatgagccgt atcacaatga atagggatgg atttagagaa ggtttttacc
    10261 tatcgcctgt tcttttttta gttactcccc accttgcaaa agcacaatca gttttattat
    10321 ttgatttagg ttaaaaaaga aagatttagc attttataaa aaaagaagat acttttgctc
    10381 ttctgtaatt ctgtagctaa tgataaaacc aaaggagcat ctgacaagcc tgaagaatcc
    10441 aaaaattcag aacatggtat ctctacacaa atcccgggag agtcgggaaa aaggggtgtt
    10501 tcttatagaa ggggttcgag agcttgaaaa agcagttaaa gcaggttatc atctaacttc
    10561 gttattttac tgcccggagc tgattagcat tgagtattta aagatatttt ccggtaaaac
    10621 tactgaggtt tacactatca ccaaagaggt ttttcaaagg attacatatc ggggtaataa
    10681 tggaggccta atagctactg ccaagcgtaa agagcatcat attatcggtt tgcaattaaa
    10741 taaaaacccg ctattactga tcctcgaatc ggtagagaaa cccggaaatc ttggcgcaat
    10801 tctccgaact gccgatgctg ccggcgttga tgccgtaata gtatgcgacc aacaaactga
    10861 cttatataat tcaaatgtta ttcgttcgtc attgggttgt attttcacga taccggtttt
    10921 tgtcgggtcg tctgccgaag taattgattt gcttaaggct aatgaaatta aaatttattg
    10981 cacctctctg cttgcttctg tgccttatta caccattgat ttcactatat ccgctgctat
    11041 tatcatggga gctgaagcta ccggcctcac ccctatctgg cttgatcatt cggatcaaaa
    11101 cattgtaatc ccgatgcgtg gtaatgttga ttcacttaat gtatcaactt cggcagcaat
    11161 tgtaattttt gaagcaatga aacaacgtag gtttgaaaag tctaaacgtg ctaaaagtaa
    11221 cctatccaaa tgattacaat gtatccgcaa tattaattga ataaatcaat caactctccc
    11281 aggttgttta aaacttttgt tccagtcgtt tcgagcctct ctttcgattg atgtccgtca
    11341 gccaccaaga tacaatcaat ccctgtttcg tgagcgactt cataatcatg aatggtatcg
    11401 ccaatcatca ctatccgatc tttggagaga cctgttttat caaccaagat atgagcattc
    11461 ccagcttttg attcagcgta atgatccctc agtccggcta ttatttcaaa ttttgaagga
    11521 tcaagtcgag cagagattgt ctcaacaaga aaatcctgat gcatagccga caatataaat
    11581 tgccttttgc ctgccgtttg aaaatagctg acaacctcat ttactctatc atgcagcaac
    11641 gacttatgaa taagatcccg ataatttctt ataaattcca aagcaattgt ttcccaatcg
    11701 tgtttttcga aatcgaagcc aactctttcg taataatccc ttacaggaaa ggtaaaaatc
    11761 tctttataat gttgtttctc aagcaatttg taacctcttt tgtgtagcat catattcatt
    11821 gcatcaatgc atatttcaag atcatcgagt aaagttccat tccaatccca aattatggct
    11881 tcataatgct ttgtaagagt gtgctccata agtgatccga aaatgttaat ttcgcaaaaa
    11941 taaacatctc agcctaatga aaataaaaag cattaatcct gccacaggtg aaataaatga
    12001 aacttatctt ctgcatactc ttccgatagt taatgatatt attaacagtg ttgatttagc
    12061 tcaaaaagag tgggtagcaa cgagttttat tgaaaggtcc atgcttatgc gaaatgcagc
    12121 caaattactt cgtaagcaaa agccgatcct agcacagttg attacccatg aaatgggaaa
    12181 agtcattcgt gagtcggaag ctgaagtaga aaaatctgca ttggtatgtg attattacgc
    12241 tgacaatgcc aaagcttttc tatccgatga ggttattaat agcgacgcaa aatcaagttt
    12301 cgtagccttt gaaccgttag gagttattct tgccgtgatg ccctggaatt ttccattctg
    12361 gcaggtattt cgctttgccg ctcccgctct catggcaggt aatgcagcag tgttgaaaca
    12421 tgcctccaac gtacctggat gctccgtagc aattgaaaag atatttaccg actccggttt
    12481 ccctgaaaat ctatttcgat cactattgat tcctgcatcc gaagtagaaa atattatcgg
    12541 gaacaaaatc attaaagctg ttatccttac cggtagcgaa tcagccggaa ggcatgttgc
    12601 aaaagctgcc ggcaaacatc ttaaaaaaac tgtcctcgaa ctcggagggt cagatccttt
    12661 tattgttttc aaagatgctg accttaataa atgcagcgaa gtagctgtta tggccagaat
    12721 gatcaatact ggccaaagct gcattgcagc caaacggttt attgtggttg aggagattgc
    12781 tgatgagttt atcaaattag taagtcaaaa gatgtcggaa ttacagtcgg aaggaccaat
    12841 ggatccaaga agtgattttg gtcccttggc aaggcctgat ctgcttatcg agctacaaag
    12901 tcaaattgat agatcagtga aaatgggcgc aaaaattgaa ttgggaggaa atccgatgaa
    12961 aagagatggt ttttactttg aacctacaat aatgtcaaaa gttactgaag atatgccagt
    13021 atttaccgaa gagactttcg gcccagtgat ggcaatcgtc gttgtggaaa atgaagagaa
    13081 ggcaatagag ttggccaaca aaagcaattt cgggttaggc gccagtatct ggacacagga
    13141 tagggacaag ggaattagat tagcaaggaa gattgaggca ggagcagttt ttatcaatgg
    13201 tttggtaaaa tccgatccga gattaccatt cggtggaata aagaattcag gatatggtag
    13261 agagctttcg aattatggaa tcaaagagtt tgtaaacatc aaaacgatat gggtaggcta
    13321 aaaaaataaa attgaaaacg ttaatagaat aataaaaaat caaattcatt ggctgtaacc
    13381 aaccaaatta ttttaaaaga aacaaaaccg atttttttat aatatcaatt tcaaatgaga
    13441 atagatatac tcacaatctt tccgcagatg tttgaaggca tattccagca ttccatactc
    13501 aaacgcgcca tatcaaagga tttagcttct atctttctgc acgatatcag ggattattct
    13561 accaataaac atcgcagagt ggatgattat gcttttggac acggcgccgg catggtgatg
    13621 atgattcaac caattgccga tctgattgaa aaactgaaat cggaacgtaa ctatgacgaa
    13681 gtgatatatc ttactccgga tggtatccct tttaagcaaa agattaccaa tcgcctttca
    13741 acacttacga acataattct gctctgcggg cattacaaag gtattgacga gcgcctgcgg
    13801 gagcatttca ttacgatgga attatcaata ggcgattatg tattatcagg cggagagttg
    13861 gcggcagccg tagttactga ttccattgta aggttgatac caggcgttct tggcgatgaa
    13921 acatcagctc tatcggactc gtttcaggat ggattgctca gcccgccggt atatacccga
    13981 ccggctgttt acaaaggatg ggaagtccca agaattttac tttcgggaga tgaaaaaaaa
    14041 atagatgaat ggctgacgga aaaagccata gagcgtacac gtagccgccg ccccgacctt
    14101 ttggaagaga tggaataaaa aacgataaac caatgacctg ttttactatt aattatcatc
    14161 agaaactatc aagtctaaaa tctgaaatat gctgaaccta ctaccaagat tttatcaatt
    14221 caaaacgaat ttaaaaaaag tgattttttt gatggtctta gtttgtggtt gtgcatcaat
    14281 aaaggccaat ccggattctc tttttataaa aaaaatagat tctattgctc tacaacttta
    14341 tagcgttcaa cccgactcga taaaactggc tgctaatgcc ataattcttg aacatattac
    14401 tgctttttta aaaaccccgc aatctattgc tttccctttt gattcgctaa aattcgtcaa
    14461 ggttgttact ccttcgaact cttctttcaa attaataaca tgggttttcc ctttatcaga
    14521 taaaaatcaa tatcactatt caggactcat tcagcacttg aaagataaca tgccagatcg
    14581 tttaacagaa ttaagacaaa gtgatgatga gccaacccct tttctcactt atcgttatga
    14641 ccagtggcct ccagccgtat attaccaatt gattgagagt aaaaccaaat ccggaacgct
    14701 gtttacgctt tttggctggg taggcggcac acagggcgaa gcagccagag tgatcgagac
    14761 attatctttt aacgaaaacc atgaggctct attcggtgtt ccggccttta aactaaataa
    14821 agagacaatt ttaaacaggg tttatttcaa ttattcgaat gaagttcctt ttcatctcgc
    14881 ttatgagcaa cattacgtcc ctggcaaaaa aaggaaaaaa gcatggatga tcgttcacaa
    14941 caggttggtt aatagcgatt ctaatatggg taacgccttc agattttcac ttccatccta
    15001 ttctgaattt gatggtttta ttttttcgga taattgctgg cagcaggtca aggatattga
    15061 tgttcgtata aaaatcaaat cagccaaaaa gaaaaaacaa cctaagaccg gcctttttcc
    15121 gaatgattaa aagggatagt ggctattagc ttcccaaaag atagcctaac caaaagggta
    15181 atccgtctgc atccggtttg tcggcaactc gtcaggcatt gttgcagcta tggcttttgg
    15241 gattcttcca aatttaataa atgctgcccg cgaggggttt gcaccgtacc tgtaaagata
    15301 gagaatgcag ccatcaatct tgggggctaa ccatggaggg ggtttcgcaa agaatcacag
    15361 cctctcgcca aaagaccttt tatctttaga atgccttcac catgaagttc agaaaacaaa
    15421 aaaaggatat agagttaaaa tttaaccgat tgagtaaact taccggtgta gctcatctgc
    15481 tgcaactctt tgtaacaaaa taggagtaat tcaggatggc cgttggatac aaaccggaat
    15541 aactaaagat gtttttcatc ttccggcaaa tgcctctgtt gcaacattta gtaggatgaa
    15601 actccaatcg gaaataatat ttctgatttt taagactttc gccgttagat ttatagaaac
    15661 ttaattgggc aactacttaa tcacagtaat ttttattgat ctgcacccaa aatcgtccaa
    15721 tatctctaca agataaaccc cgctccttat ttgagacata tctaatctta catttgtcgc
    15781 attgatatcc ttttcgtact ttattaagct tccaaataaa tcaatcacct ttacttcttt
    15841 aatcatcata tcggatgtga tggttatctt gtcatttttt accggattag gatagacgga
    15901 gacgtttccg gcatatctct tttcaatacc gataccgcta tcaactttaa taaagttttg
    15961 aattaacagt gagtcaatac cataatcatt ttggactatc agttttacgt cgaactctcc
    16021 caattcataa taggtaattg atggattctg caaattggaa acaggaggtg tacctccctc
    16081 aaacgaccag ctccaggatg ttggattatt tgttgagagg tcggtaaaca taaccgtatc
    16141 tcctctataa atcagatttt ggttagcgct aaaatcggct attggaggca gcccgacaaa
    16201 gctacagctt aatatagctt tccatccgga tttgttcacc gaatagtcgg aatggaactg
    16261 aaatgtaagc gctccctgat cgtttgaggc ttcaattgta cctggcgaat ttgtaccgca
    16321 atatgtgcca atcaacgacg ccgaagtatt tattccatta tatattttta gccagtccca
    16381 gttacaggtg gagttatatt caacatcaaa ataaataaac tgaactatga tattagctcc
    16441 tgccgtttca ggcttaaagg tcattaaata atcttcatta ttggaataat tacctgtatc
    16501 tccaccagaa tcataaaaga taccattgca aattgttacc gtttgattac tcatcaaaaa
    16561 agtggcgttt actgtaatat aatcctcttt cgttatggtg tttgtctcac cgttttcatt
    16621 ggcaactgtt aatgagaccg gaaaagagcc tgcattagga taagtaatgt tttgaggatg
    16681 ttgcatattg gatgttgaag gtattccacc agagaaactc cagctccagc tcacgggggc
    16741 gccatacgat tcgtcagtaa aattgatggc gtcaccaata gcgatagtcg tttcagaagc
    16801 cgaaaaacct gcttctaact cgccggcatc catctcgaca tttaagttaa cggtattgta
    16861 tctggtaaca tttacattat tgataacaac cggatagtat ccaggggcgg taaaggtaag
    16921 attgtaagtt ccggcgtcaa gcaatcgttg ataaaagcca gtttgttgct ctgaaaagac
    16981 aaatgaatta tgaatatcat gcccttcgat ggtaattttt gcattgatgg gctggccggt
    17041 agttatgttt gtaactgttc cattcacacc ataaagactt tgttccaaat agttcaacaa
    17101 cgagcggtag ttccagttcc aatgattttc aagttgcgaa gtaggtaaga gcttaacatt
    17161 agaaatttcc atggttacct ctcggcagtt atggaagtaa ttcatataat cttgtctgcc
    17221 gccactgatt gaataccagc tatatccatt tattatccca ttgttaaact gattcatgta
    17281 gtttggaggt gaatagagat ggactgtgtc aacatattgc ctgcatacaa aaacccacca
    17341 ggtattatcc gccggcaatg gcgaccatgt atcccaaggg tagttaagta cctctgctcc
    17401 gccatgggta tttgccgaca tgacaaaatg ttgctcttca gccaactgca taaatgcaac
    17461 ggtttcgggt tgccattcgt ttccatcagg atgcgggcca tcttcagggt caggataatt
    17521 acgatttaaa tctacattat tggcattata cctcgttgcc ccatatactg agttatttcc
    17581 ccccttgtaa gtaccatcgg gattacttaa tggattaatc catatttcgc tattatccaa
    17641 gaggttagta acttttgaat tagttccgta attgctgagc aaatagtcaa tcaatctgag
    17701 cagtaagata tagccggtgg tttcgtcgcc gtgcattgtg ccggtataaa gaaactgagg
    17761 ctccgcttcg cgaacctgta cattttttga caccttagcc atcaacaatt ctctcccttg
    17821 aatactctgg ccaatactaa aaacttgaca aagttgagga tattcgctgg caaattgata
    17881 catcatatct acatatgccg ggtacgttgg atagaaatcc caatctaaga tgcctttaac
    17941 attgacgttg tcaagcattt taatctcttt tgtcaattct cctggctttg gcagtagctc
    18001 gaaatcaatt ccttgtttga gaaattcgcc aaattccgtt ttgttagcat aagcaaaaac
    18061 gtcatgccaa ttgactttat ctatcgaaat cattttcgat aatgcttcaa ggtttatttc
    18121 tttattttcg aacttaaagt aaatctcgcc ctttgatcca aaaatttcat caacggtagt
    18181 tacattgtta ccagtttgag cgtagataca aaatatcaac aatgtgataa atgttaagag
    18241 aagatatttt ttcaaaatca tgattttgct atcttatggt ttattaattt tgatattcgg
    18301 cgataagctg ctgaagctgt aaatttacgg ctttgtttac tttaactaat ggaaggcgga
    18361 gattgttagc ggcaatttct aaaatttgca gcgccgcctt aattcctgca ggactaccat
    18421 cttcaaaaat cttttgcatt ataggtaaga gcttgtaatg tattacatta gcttctgcaa
    18481 attttccctt caggcataaa tttaccatct ttgaaaagtc ggatgggaag gcattagcga
    18541 ctaccgaaat aacgccatta gctccaaggg agatcaatgg aagtgttatg gcatcatcac
    18601 ctgaaatgac atggaaatta tcaggtttgc atcgtattat ttccataatt tggttaacat
    18661 ttcctgaagc ctcttttacc gcaacaacat tatcgcattc ggaagcaagc tgtaacgtag
    18721 tttccgccga catattagcg cctgttcgtc caggtacatt ataaaggatc attggaacag
    18781 gacacacact cgaaaccgtt ttataatggt aataaatacc cttttgctgc ggtttattgt
    18841 aatacggact tacagataga ataccggcaa tgccctcaaa agacatagtt tttattttat
    18901 caatgatctc ctgcgtattg ttacctccta ctcccaccac taccggaacc ctgccagccg
    18961 cacattcaat aacaaaatca actactgcta acttttcatc gttggaaagt gtaggcgtct
    19021 caccggtagt acctaatgcc actaagtagt caacattacc tgcaatcgtg tgttcgacaa
    19081 ctttttcaag tgaacaaaaa tcaatatttc catttttgtg aaatggcgta actaaagcaa
    19141 cacccgtgcc tgtgaaatta aacttcataa ttactttgaa ttaattgtac gtaaataact
    19201 taccattgtt tcagcaaact ctctactctc cattccatca gtatttttta tcataaaatc
    19261 aaatacctca accatttttt gagtaaagca tccggttttg aaatttgcat gagaatagcc
    19321 cgaaatataa ttaagcggaa ggatatctcc tgcgctgaaa tcaatcagca aatcaaaatc
    19381 tttctgaata aaactttcga caaaaggctt tttacaaatt ccaaggagat taagatcttt
    19441 ggatgtgatg atatccattt tcaatttggc aaggtaataa ttcggaattt gtttcatagg
    19501 taagtaggcg ataatacta
//
LOCUS       DAOOAI010000036        18804 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_3902_length_18804_cov_5.430743, whole genome shotgun sequence.
ACCESSION   DAOOAI010000036 DAOOAI010000000
VERSION     DAOOAI010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18804)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18804)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18804
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3902_length_18804_cov_5.430743"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..506
                     /locus_tag="PK345_03990"
     CDS             <1..506
                     /locus_tag="PK345_03990"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="mechanosensitive ion channel"
                     /protein_id="HPX34216.1"
                     /translation="LFAILFLIVAYSAWVGLKDIIAGAFLKATHKLSINELITVGDYS
                     GKIVGFDHSNLLLETALGETLYIPYSSLLGKVIVKMQPAESIHRHSFKFEVDKAHSTR
                     EDTEKIRGFILTMPWASLKKEPQIRYVTDTTSDKQIIEVTLYSIEKEHFQDMERLIKE
                     KFSTSKD"
     gene            complement(581..1033)
                     /locus_tag="PK345_03995"
     CDS             complement(581..1033)
                     /locus_tag="PK345_03995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34217.1"
                     /translation="MKSKIIAIVLPTLLGVLAVIGLLILFNLIVYNGDGFNSPDNGFF
                     TLIVPVTTIIAMIIQCVLTLPLWKKFKSKKRVLGMTIIQLTGLLCLMSGLAFGLVFWE
                     RSFGIMELILLSLSGIISFSVYWSVNLITLNLLDKQMVDKHFRVICNN"
     gene            1302..4166
                     /gene="gcvP"
                     /locus_tag="PK345_04000"
     CDS             1302..4166
                     /gene="gcvP"
                     /locus_tag="PK345_04000"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HPX34218.1"
                     /translation="MLTNNFIRRHNGISESEYPEMLKMVGAATLDQLIDETIPEKIRL
                     QHPLNLPDGINEYEYLAHLKSLGAKNKLFKTYIGLGYYNTIVPGVIQRNILENPGWYT
                     AYTPYQAEISQGRLEALLIFQTMIADLTGLPIANASLLDEGTAAAEAMIMAFNSRSRE
                     AVKSGANKFFVSKNLFPHSIEVLKTRSKPLNIKLIIGDHESFEFTKDVFGAIVQYPDQ
                     HGEIYDYSKFTEKAHSIGAMVAVAADLLSLSLLTPPGEWGADIVLGSSQRFGTPMGFG
                     GPHAAFFSTKEEFKRNIPGRIIGVSVDANGQPALRMALQTREQHIKRERATSNICTAQ
                     ALLAIMAGIYGAYHGPNGIKSIAEQIHKLTANLAEQLEKLGLKRINKFFFDTLTFETS
                     NVEVVEKTRRIALKNQVNFRYDNTLIGISLDETTSETDVKKIVEIFAEALGKPLISFE
                     FSDKIQLPENLIRSTPYLTDPVFNSYHSETEMMRYLKRLENRDLALNRTMIPLGSCTM
                     KLNAAAEMFSLSWPEFNNMHPFVPEDQAEGYLELIKDFEKDLCEITGFAAVSFQPNSG
                     AAGEYAGLLVIREYLIKTGQGHRNICLIPASAHGTNPASAVMAGMEVVVVKSDSNGNI
                     DVNDLREKAEKHKDNLATLMITYPSTHGIFEQDVLDIINIIHQNGGQVYMDGANMNAQ
                     VGLTNPGFIGADVCHLNLHKTFAIPHGGGGPGVGPIGVASHLAPYLPGHKLVKTGGKD
                     ATTAVSSAPFGSAMILPISYGYIKLMGGKGLTLATKIAILSANYLKNALNDHYKILYT
                     GKNDRVGHEMIIDCNEFNKTAGITVIDIAKRLMDYGFHAPTVAFPVHGTLMVEPTESE
                     PLTELKRFVDAMISIKKEILEIENGNADKTNNLLKNAPHTLCMVISDEWTLPYSRTKA
                     AFPIDCSKADKYWTPVTRIDEAYGDRNLVCTFDNNSAFES"
     gene            4197..4904
                     /gene="tsaB"
                     /locus_tag="PK345_04005"
     CDS             4197..4904
                     /gene="tsaB"
                     /locus_tag="PK345_04005"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HPX34219.1"
                     /translation="MPLILNIETATQYCSVALFDGNRTLLEQSKEKNAHAEVITAMIE
                     KLLIEAGAIPSMLDAVAVSMGPGSYTGLRIGVSTAKGLCYALDKPLIAISTLQALAYG
                     SRNHIKNNPEYSENTLFCPMIDARRMEVYSALFDINIRQVRETKAEIITENSFSEELQ
                     LGHVIFSGDGATKCKSFFKQKPNAIFLDDFLPSASFMVEIALKKYNKNAFEDIAYFEP
                     FYLKDFIAAKPKVKGLY"
     gene            complement(4941..6323)
                     /locus_tag="PK345_04010"
     CDS             complement(4941..6323)
                     /locus_tag="PK345_04010"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPX34220.1"
                     /translation="MPDRNLNIDKLFKDYLKNYQTQAPEGAWERIQADLKKERRVAPI
                     WLWRVAAALILLIMTFAAGYFLSQYQRDNTKLPIAEKHTPASIETPASKTEVRKVEKT
                     YPVEQVTSTSEEKDKKQVDTHVKQPAVTTKEDVKSDLIPETILPEEDVALDLLPALDG
                     KVSDELIEDMAETIKEPLYGEKEIFDRIEYDAHYVRDLLDQEEYTDVLAQKEKKSSSG
                     NWGIGPRLAPVYSYRLLGSNASGESDKSYFNDVENSIMNFGAAISLSYSFNNKWSLKT
                     GMYVSRIGQENKQVLAIVDEGRSGMFKLSTSSGAVFINPKKFESVMIQQQVSSKDTVS
                     GGYLVNGSFIQNLDYFEVPIVMQYKVVDQRFAVNILGGLSPGILVNNRSYFESGKDKL
                     QTGIVENVAPAVLNSLIGVGFDYAISKNVYLNMEPSFRYSISSINAGNDLRNHPYSIS
                     WLTGITYKFY"
     gene            complement(6316..6870)
                     /locus_tag="PK345_04015"
     CDS             complement(6316..6870)
                     /locus_tag="PK345_04015"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPX34221.1"
                     /translation="MDQQKVIEGCIKGIAKAQQELFNHFANEMFGVCIYYSSNRVDAE
                     DTLHEGFLKVFQNIKSFKGKGSLKGWIRRIMINTALEKYRKKSPLYLLDDQYIESAND
                     INRDDIVSNITADDLIGLIQELTPQYRAVFNLYALEGLTHKEIAEKLNISEGTSKSNL
                     SRARTILQQKVKAHFYVSSKNINA"
     gene            complement(7404..8594)
                     /gene="kbl"
                     /locus_tag="PK345_04020"
     CDS             complement(7404..8594)
                     /gene="kbl"
                     /locus_tag="PK345_04020"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006567 - threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HPX34222.1"
                     /translation="MYGKIKDHLQKELAAIQEAGLFKHERIIVSPQQAEIKLDSGQTV
                     LNFCANNYLGLSNHPALIQAAKEALDTHGYGMSSVRFICGTTDLHKTLERKIAEFFGT
                     DDTILYAACFDANGGVFEPLLGEEDAIISDSLNHASIIDGVRLCKAKRFRYQNADVKD
                     LEAQLIAAQDCRFRIVVTDGVFSMDGNVAPMDKIVELCKKYDALVMVDECHSAGVVGE
                     TGRGVTELFNIRGEVDIITGTLGKAFGGAIGGFTTGKKEIIEILRQRSRPYLFSNSLP
                     PNVVNAGIKMFDMMSETHELQDKLHDNTEYFMSKMTAAGFDIKPTKSAICAIMLYDAK
                     LSQVMASRLLEEGIYVIGFYYPVVPKGLARIRVQLSAGHERKHLDKAIDAFIKVGKEL
                     GVIK"
     gene            8884..10416
                     /gene="purH"
                     /locus_tag="PK345_04025"
     CDS             8884..10416
                     /gene="purH"
                     /locus_tag="PK345_04025"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HPX34223.1"
                     /translation="MNNLTSKKISSALISVYDKTGINTIVKALLKNDVEIISTGGTYD
                     YLTKKGFSCKKVEEITTYPSILGGRVKTLHPLIFGGILSRRDNQDDQHELEQYKIPTI
                     DLVIVDLYPFETTLINTQIEQKINEMIDIGGISLIRAAAKNYKDVVVVPSVKYYSHFL
                     EIYSNNDGRFTIEERKFFAACAFNISSHYDTAIFNWFNREVKLQVFKQSITDVSHLRY
                     GENPHQEGVYFGNITELFDQLNGKAISYNNLNDIEAAIQLANEFDEVLVAIIKHNNAC
                     GLACRQSLTEAWGAALESDPLSAFGGVIITNRAVDYHTALEINKLFFEVIIAPDFEQK
                     SLKLLKQKKNRIILRQKVFALPKLQFKSALNGVLVQDKDLVEEDKIKLDFVTKSKASS
                     EEIEDLLFANKIVKHTRSNAIVLAKNKQLLGSGIGQTSRIDAVKHAIEKAKSHGFDLQ
                     GAVMASDAFFPFSDSVEMAYKEGIRAIIQPGGSLRDADSINYCDQTGMSMAFTGIRHF
                     KH"
     gene            10478..11500
                     /locus_tag="PK345_04030"
     CDS             10478..11500
                     /locus_tag="PK345_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004304915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HPX34224.1"
                     /translation="MGLFSFFTKEIAIDLGTANTIIIYNDKVVVDEPSIVAIERSSDR
                     IIAIGKRAQMMHGKTHENIKTIRPLRDGVIANFQAAEYMIREMIKMIGIRGSIFPPAL
                     KMVICIPSGITEVEERAVKDSAEQAGAKEVKLIHEPMAAAIGIGIDVLEPTGNMIIDI
                     GGGTCEIAVITLGGIVNNKSIRVAGDDFNADIEDYMRKQHNINIGERTAERIKIEVGA
                     ALPELDNPPDDYAVHGRDMLTGIPKEIIVNYTEIAHCLDKSILKIETAVMQALEMTPP
                     ELSADIFRTGVYLAGGGSMLRGFDKRLHLKTKLPIHVAEDPLRAVARGTGIALKNYDK
                     FTFLIK"
     gene            11526..12371
                     /gene="mreC"
                     /locus_tag="PK345_04035"
     CDS             11526..12371
                     /gene="mreC"
                     /locus_tag="PK345_04035"
                     /inference="COORDINATES: protein motif:HMM:NF010532.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HPX34225.1"
                     /translation="MQNLFAFIWKNSFYFLFVLLLVLSVVLMARNNYYHRTVMINTTN
                     EITGGILLVASNFSDYMKLKRTNRELAEENAKLISQTKELFLDASTKAIPSPDSNVLI
                     PQYEFIAAKVISNSVNRRNNYIKLDKGRFQGIMPNMAVISPAGIVGHIVEVSNHFSSV
                     MSVLNRNSRISAKHKTSNHVGSLLWDGIDYRFGKLVDLPSHVKIFQGDTIVTSGYSHL
                     YPEGVIIGTVQEAILESGASFYNVTVSFSTDFNRLSYVYVVNNLFYNELDSLQQLQPQ
                     NEPLR"
     gene            12368..12874
                     /locus_tag="PK345_04040"
     CDS             12368..12874
                     /locus_tag="PK345_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HPX34226.1"
                     /translation="MIIQNVIRFIAALLLQGLIFNQIGIGAYLFPMFYVYYILMLPFE
                     TKGWVLLITSFLLGLGVDFFSNSLGLHSTASLCMAFFRPGVIRLLTVGKDYETLVYPS
                     IKKFGFSWFFSYTVILVIIHHTIFFILEVFSFYEIRQIIFRILISSIATIFLIFITQL
                     LFLKREKH"
     gene            12919..14004
                     /locus_tag="PK345_04045"
     CDS             12919..14004
                     /locus_tag="PK345_04045"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPX34227.1"
                     /translation="MKKSFLAIILAMFLFSCSTPENQFEIIGNLPEASDNWVVLAKAV
                     DNDLKTIDSVMVKGGKFYFKGIIDFPEMYYLYFKADDKYLGFFIEPGKMNISGTFDEP
                     IYDGLPIQALYDKLMHDLKSWEGQFEILSAQYREASAKGDLDMQKAIEEKAELIETER
                     NEHIMKFIEENSNSVIAPFLLYNNIHSFDIEKVEAITNVIDSSVHKSVYYTLLNDELE
                     KLLKVSIGRMAPLFSQNDPDGNPVALESFRGKYLLIDFWASWCQPCRLENPNLVANYA
                     KYRARGFEIFGVSLDRDRAAWLKGIADDNLTWPQVSDLQFWNNEASRLYGVNSIPANF
                     LLDPDGIIIAKGLRGKQLEDKLAEIFP"
     gene            14540..15118
                     /locus_tag="PK345_04050"
     CDS             14540..15118
                     /locus_tag="PK345_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005945048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HPX34228.1"
                     /translation="MKSIKGTQTEKNLLKSFAGESQARMRYTYFAKEADKEGYKQIAA
                     IFMETAEQEKEHAKRMFKYLEGGMLEITSTYPAGVIGTTAENLEAAAAGENEEWTKLY
                     PHFADVAEEEGFKEIATMYRMIAKAEAIHEERYLKLLQRVKDETVFSRDEEIEWQCRN
                     CGYVHKGKKALKTCPACLHSQAYFEPKCDKYY"
     gene            15356..16288
                     /locus_tag="PK345_04055"
     CDS             15356..16288
                     /locus_tag="PK345_04055"
                     /inference="COORDINATES: protein motif:HMM:NF015226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urea transporter"
                     /protein_id="HPX34229.1"
                     /translation="MTKKLNSKNSLLGKSASIISVILKGFGQIMLQENSITGLLFLIG
                     IFYGSLAMGFAALLATVCGTATAYLLKYDRTEIKKGLYGFSAALVGVAVLLFLKPLFW
                     AWIIVGVGSVLATMLQHFFIKRKIPAFTFPFVLITWLILFICNNFYSDLLAIPVPTIA
                     HLSDSLSDGFKSFGQVIFQNKLVSGLLFFIAVFISSPIAALYGLAGAIVSDIIAFEFS
                     APIDNINLGLYGFNAVLCAIVFAGNQVKDGIWAFLSVVLSLGISLLMIKFGLTQLTFP
                     FVFATWITLFLKNRLQKPINAVIFKDRTKPTNSQ"
     gene            complement(16364..16966)
                     /locus_tag="PK345_04060"
     CDS             complement(16364..16966)
                     /locus_tag="PK345_04060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HPX34230.1"
                     /translation="MKTKILFIFLICLGYSMNAQEIKLPAPDRSGGQPLMKSLNERKS
                     ARSFSEKDLSDQQLSDLLWAANGFNREDKRTAPTARNCQELELYVILKKGIYFYNAKE
                     HKLTLVKDGDHRKSAGIQDYVADAPLNILIVSDSEKAASSQYSYTNCGYISQNIYLYC
                     ASEGLATVARGAFNKEELHSLLQLPKSHEVLLAQTVGFPK"
     gene            complement(17130..18269)
                     /locus_tag="PK345_04065"
     CDS             complement(17130..18269)
                     /locus_tag="PK345_04065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008779068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BPL-N domain-containing protein"
                     /protein_id="HPX34231.1"
                     /translation="MKLLLPIIFLLFFVQCTQKQTTDQELNNKIQVAVFDGHGGAQTC
                     IWETVAAVKLDTDMSVRTITTADIANNVLDSLDAIIIPGGGGSRQFLNLSALNQQRIK
                     DFVASGGGIVGICAGAYLLSNTPDYACMQLNGAQAIDIEHDNRGHGISKFTLTDEGKR
                     IFPEIAKRDTLFVMYYEGPVFIKKENDTISYETLAIMESDVHEEGNAPKNMTNNKPFF
                     ITNSYGKGRIFSSIAHPEATPGMMWMIPRMVRWTLNLPIKEYKSEVVNPDIFNREILM
                     SISDLKQESAYYQTFLYGSAEEKIVAMDWLQSKHSWAAKRWVQGLLFDADASVRVRAA
                     KYIADTHYIHYLPDLRAAYNSEKETQTKQQLKEQLDKLENIRDGF"
     gene            complement(18536..>18804)
                     /locus_tag="PK345_04070"
     CDS             complement(18536..>18804)
                     /locus_tag="PK345_04070"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPX34232.1"
                     /translation="FYSALTDGSQKAMISDIVSKELKGTGFGIYHAVLGITLLPASLI
                     AGLLYDRVNANAPFYFGSVMAFIATILMIIFTVVDKRKQKLTDA"
BASE COUNT         5867 a         3691 c         3462 g         5784 t
ORIGIN      
        1 ctttatttgc aattctgttt ctaatagttg catactccgc ctgggtaggg cttaaagata
       61 taattgcggg cgcttttttg aaagcaactc acaaattatc aatcaatgag ctgataacag
      121 taggcgatta ttcaggcaag attgtcggtt tcgatcactc caacttattg ctcgaaacag
      181 ctcttggcga aacactctat ataccgtaca gctcgttact tggaaaagtg attgtcaaaa
      241 tgcaacctgc tgaatcaata caccgccact cgttcaagtt cgaggttgac aaagctcact
      301 ctacaaggga agatacagaa aaaatacgag ggtttatcct taccatgcca tgggcttcgt
      361 taaaaaaaga accacaaata aggtatgtta ccgataccac ctccgacaag caaataattg
      421 aagttacgct ctattccatc gaaaaggagc attttcagga tatggaaagg cttatcaaag
      481 agaaattttc cacatctaaa gactaacccc caaaaattag ttcagctaaa aaaggatttt
      541 tttgacagcc ttacgagcag gcgctttacg ttggagtttt ctagttatta cagattactc
      601 tgaagtgttt atccaccatc tgtttatcta acagattcaa tgttataaga ttaacactcc
      661 aatatacgga aaatgaaatt attccagata gagacaataa aattagctcc atgattccaa
      721 aacttcgttc ccaaaatacc aatccgaaag ctaagcctga cattaaacaa agtaatccgg
      781 ttaactgaat aattgtcatc cccaaaaccc tcttcttaga tttaaatttt ttccaaagag
      841 ggagggtcaa gacacactgg attatcattg caataatcgt tgttaccggt acaattagcg
      901 taaaaaaacc gttgtcgggt gaattgaaac catctccatt gtacacaata agattaaaca
      961 atattaatag tccaatcact gccaaaacac cgagcaatgt cggtaaaact atcgctatga
     1021 tttttgattt catatttttt atttttaaac aatcaaatca ttaagatatt attcacgttt
     1081 gatatctttt ttattaagac acaaagacac ttaccaaact caacaggcat aaaaagcggc
     1141 cgatttttgc aaaatatcca agaagacgga ggatattgaa aacgctactg catttaacac
     1201 atctttacac agataaaaaa aatgaaaaaa tcacccaaag aattgtacct ttgcacttga
     1261 tttctaaaaa gttaataata aatttaaatt ctaaacttaa catgctcact aacaatttta
     1321 taagacgtca taatggaatt tcggaaagtg aatatccaga aatgctgaag atggttggcg
     1381 ctgccacatt agatcaactt attgatgaaa ctatccctga aaagatcagg ttgcaacatc
     1441 ctttaaatct tccagatggg atcaatgaat atgagtatct ggcgcattta aaatcgttgg
     1501 gagcaaagaa caaactattc aagacataca tcggtcttgg ttattacaac acaattgttc
     1561 caggggtcat tcagcgtaat attcttgaaa accccggttg gtacactgcc tatacacctt
     1621 atcaggctga gatttcacaa ggcaggcttg aggctcttct catatttcaa actatgattg
     1681 ccgatcttac cggcctgccc attgcaaacg cttcgttact cgatgaaggt accgctgctg
     1741 ccgaagcaat gataatggct ttcaactcac gttctcgcga agcagtaaaa tcaggtgcaa
     1801 acaaattttt tgtcagcaaa aatctattcc ctcattcgat tgaagttctt aaaacccgtt
     1861 caaaaccatt gaacataaaa ttaatcattg gcgaccatga gtcttttgag tttactaaag
     1921 atgtctttgg agcaattgtt caatatcctg atcagcatgg tgaaatttat gattactcga
     1981 aatttactga aaaagcacat tctatcggtg cgatggtagc tgttgccgcc gacctgctca
     2041 gcctcagcct gctcactcct cccggcgaat ggggcgccga tattgttctt ggatcaagtc
     2101 agcgttttgg aactccaatg ggatttggcg gaccacatgc agcattcttt tccaccaaag
     2161 aagagtttaa aagaaacatc cctggaagga ttatcggcgt ttctgttgat gctaacgggc
     2221 agccggcttt acgaatggca ttacaaaccc gcgaacaaca catcaaacgc gaacgtgcga
     2281 cttcaaacat ctgcacagca caagccttgc tcgcaataat ggctggaatt tacggagcct
     2341 atcacggccc aaatggtata aaatcaatag ctgaacaaat acacaaactt acggcaaacc
     2401 ttgctgaaca gcttgaaaaa cttggactta agaggataaa taaattcttt ttcgacactc
     2461 tcacttttga aacttcaaat gtagaggtgg ttgaaaaaac gagaaggatt gcacttaaaa
     2521 atcaggttaa tttcagatac gacaatacgt tgatagggat aagcttagat gaaaccacaa
     2581 gcgaaacaga tgtaaagaaa attgttgaga tttttgctga ggcattaggt aaaccgttga
     2641 tctcttttga gttttccgat aaaatacaac ttcctgaaaa cctgatcaga agcacccctt
     2701 accttactga tccggtattt aacagctatc attccgaaac tgagatgatg cgctatttaa
     2761 agaggcttga aaatcgcgat ctggctctca accgtacaat gattccactt gggtcgtgca
     2821 caatgaaact caatgctgct gccgagatgt tttcgctatc gtggcctgaa tttaacaata
     2881 tgcatccatt cgttccggaa gatcaggctg aaggatacct tgaactgatt aaggattttg
     2941 aaaaagatct atgtgaaata accggttttg ctgcagtctc ttttcagccc aattcaggcg
     3001 ctgccggtga atatgcaggt cttttagtga ttcgtgaata tctgataaaa acgggtcagg
     3061 gacatcgcaa tatatgcctg atccccgctt cggcacacgg caccaacccg gcaagcgctg
     3121 tgatggcagg catggaggtt gtcgttgtaa aatcggattc taatggaaat attgatgtaa
     3181 acgacttgcg cgaaaaggcc gaaaaacaca aagacaattt agcgactctg atgattacat
     3241 atccttcaac gcatggcatc tttgaacaag atgttttgga tataatcaat attatccatc
     3301 aaaatggcgg gcaggtttac atggatggag caaatatgaa tgcacaggta ggccttacta
     3361 atcctggttt tatcggcgca gatgtttgtc atttaaatct acataaaact tttgccatac
     3421 cccatggcgg tggcggcccc ggcgttgggc ctatcggtgt agcaagccat ctggcgcctt
     3481 atctgccagg ccataaactg gtaaaaaccg gcggtaaaga tgccacaact gctgtctcct
     3541 cagctccctt tggcagtgcg atgatcctac caatatccta cggttatata aaactgatgg
     3601 ggggtaaagg cttaactctt gcaaccaaaa tagcaatcct aagcgctaat tacctgaaaa
     3661 atgccctgaa tgaccattac aaaattcttt ataccgggaa aaatgaccgt gtcggacatg
     3721 agatgatcat tgattgtaac gagtttaaca aaactgctgg tataactgtg attgacatag
     3781 ccaaaaggct tatggattat ggcttccatg cacccacagt agcattccca gtacacggta
     3841 ctcttatggt tgagcctact gaaagcgaac cgcttacaga attgaagcga tttgtagatg
     3901 caatgatttc cataaaaaaa gagatattag aaattgaaaa cggtaatgca gataaaacaa
     3961 acaatctttt gaaaaacgca cctcataccc tttgtatggt tatttccgat gaatggaccc
     4021 taccctattc acgaacaaag gcagcatttc ccatagattg cagcaaagct gataaatatt
     4081 ggacaccagt aacacggatt gacgaagcct atggcgaccg caatttagta tgtacttttg
     4141 ataataactc tgcttttgaa tcataagctt aacagtctgt ttttagtttc cgttctatgc
     4201 cccttatcct gaatatcgaa actgctactc aatattgctc tgtagctcta tttgatggaa
     4261 acaggacttt attggagcaa agtaaagaga aaaatgctca tgcagaggtt atcaccgcga
     4321 tgatcgagaa gcttttgata gaggcaggcg caataccttc aatgctggat gctgtagcgg
     4381 taagcatggg gccgggctcc tacaccggat tacgtattgg cgtatcaacg gcaaaaggcc
     4441 tatgttatgc actggataag ccattgatag ctataagtac tttgcaggcc ttagcctatg
     4501 gaagcaggaa tcatatcaaa aacaatccgg aatactctga aaacactctt ttctgcccca
     4561 tgattgatgc ccgaaggatg gaagtctatt cggcattatt cgatattaac attcggcagg
     4621 taagagaaac gaaggcggaa attattactg aaaattcatt ttcggaagag ttacaattgg
     4681 ggcatgttat tttttcagga gatggcgcta cgaaatgcaa aagctttttt aaacaaaaac
     4741 ctaacgccat ctttttagat gattttttac cttccgcaag ctttatggta gagattgcat
     4801 tgaaaaaata caataaaaat gcctttgaag atatagctta cttcgagcct ttttatctaa
     4861 aggattttat tgctgcaaag ccaaaagtga aagggttata ctaaaaaaag aggagcaaaa
     4921 agtgatgaaa gggtcaacaa ttagtaaaat ttgtaggtga tcccggtaag ccaggagatg
     4981 gaatatgggt ggtttcgcaa atcattgccg gcgttaatag agcttattga atacctgaac
     5041 gaaggttcca tattaagata aacattttta gaaatcgcgt aatcaaaacc gacgcctatc
     5101 aggctattaa gaactgccgg agcaacattt tcaactattc cggtttgtag tttatccttt
     5161 ccgctttcga aataagagcg attgtttact aatattcccg ggctaagtcc gccgagaata
     5221 tttactgcaa atcgttgatc cactacttta tattgcatca caatcggcac ttcaaaatag
     5281 tcgaggtttt gaataaatga gccatttacc aaatagcctc ccgatacagt atcttttgaa
     5341 gagacctgct gttggatcat taccgattcg aatttctttg gattaataaa aaccgctccg
     5401 gacgaagttg acaatttaaa catgcctgat cttccctcat caactatcgc taatacctgt
     5461 ttattttcct gccctatgcg cgatacatac attccagttt taaggctcca cttattatta
     5521 aatgaatagc taaggcttat agccgcacca aaattcatta tgctattttc aacatcgttg
     5581 aagtatgatt tgtccgattc acctgaagca tttgagccaa gcaaacgata ggaataaacc
     5641 ggagcaagcc gagggccaat accccagttt cctgaggaag attttttttc cttttgagcc
     5701 aaaacatcag tatattcttc ctgatcgagc aaatccctga cgtaatgggc atcgtattct
     5761 attctatcga aaatttcctt ttccccgtaa agaggctctt taattgtttc ggccatatct
     5821 tcgataagtt catcactcac tttaccatct aaggccggta acaaatccaa ggcaacatcc
     5881 tcttccggga gaattgtttc aggtatcaga tcggatttta catcttcttt agtagttacc
     5941 gcaggctgtt ttacatgagt atcaacttgt tttttatctt tttcttcgga tgttgaagtt
     6001 acttgttcga ccggatatgt cttttcaacc tttcgaactt cggttttcga agcaggtgtt
     6061 tcaatacttg ccggtgtatg tttttcggcg attggcagtt ttgtgttatc tctttggtat
     6121 tgtgataaaa agtatcctgc agcaaaggtc ataatcaaca agataagtgc agcagcaacc
     6181 cgccatagcc aaatgggggc aactctgcgt tcttttttca gatcagcctg aatccgttcc
     6241 catgcgcctt cgggcgcctg agtctgataa tttttcaggt aatccttaaa taacttgtca
     6301 atatttaaat ttctgtcagg catttatatt ttttgatgaa acgtaaaaat gtgctttgac
     6361 tttttgctga agtattgtgc gtgcacgcga aaggtttgac tttgatgtgc cttcagatat
     6421 attcaatttt tcggcaattt ctttatgcgt aaggccttct aaagcataaa ggttaaaaac
     6481 cgctctgtat tgtggagtaa gttcctgtat taatcctatg agatcgtcgg cagtaatgtt
     6541 acttacaata tcatctctat taatatcatt tgccgattca atatattgat catcaagaag
     6601 atataatggg cttttctttc tatatttttc aagggcagtg ttaatcataa ttcgccttat
     6661 ccatcccttt aatgagcctt ttcctttgaa tgacttaatg ttctgaaaca ctttaagaaa
     6721 accttcatgc aaagtatctt ctgcatcaac tcgatttgag gagtaataaa tacagacccc
     6781 aaacatctcg ttggcaaaat gattgaaaag ctcctgttgt gctttagcta tgcccttaat
     6841 gcaaccctct attacttttt gttgatccaa tattgaatcc tgtttttgaa atggccaaaa
     6901 taagtcaaaa cattcaaaga acgcttgtca aagacttata ttagacgtac taataaaaat
     6961 tttggttgcg taactctata tttccaaatt tttcgcagca ataaaaaaat cctttgatga
     7021 actatacgca accaattttt aaaacgaatc atctaagcat tatcaaataa taaatcaatt
     7081 gtatgtcaac catcaaaaat ttgattttag ccattggtaa aaatgataag tttaaaataa
     7141 actcaaaact atcattttac atattgttag caaccttttt tattttactc attgcagcag
     7201 aaagtattat taaacaaacc tttatatctt tttaagagta gttttttttc atggttacaa
     7261 ataaagtagt taatggattg atttgattgt aaaaattgag ccacagactt tgagaaaatc
     7321 tgtggctctt ctatttttct cggaatttaa aaggcaatct taattttaag gctgcctttt
     7381 ttgttaaaat cctgctacca atcttattta attaccccta actctttccc aactttaata
     7441 aatgcatcaa tagctttatc aaggtgtttg cgttcgtggc ctgccgatag ctgtacccta
     7501 atacgtgcaa gtcctttcgg tactactgga taatagaaac ctatgacgta aattccttct
     7561 tcaagcaatc tggaagccat tacttgcgaa agttttgcat cataaagcat aatagcacag
     7621 atagctgatt ttgtaggttt gatatcgaag cctgctgcag tcattttcga cataaaatac
     7681 tctgtattat cgtgaagctt atcttgcagc tcatgcgttt ccgacatcat atcaaacatc
     7741 ttaataccgg cattcacaac attcggtggt aatgaattgg aaaacaagta aggccttgag
     7801 cgttggcgca ggatttcgat aatctccttt ttaccggtgg taaaaccgcc aatagctcct
     7861 ccaaacgctt tcccaagtgt tcctgtaatt atatccactt cgccacgaat attaaacaac
     7921 tcagttacac cacggcctgt ttcgcctaca acgccagccg aatgacactc gtcaaccata
     7981 accaatgcat catatttttt acaaagttca acgattttat ccattggcgc cacattgcca
     8041 tccatagaga aaacgccatc ggtaacaact attctgaaac ggcaatcctg tgcagcaatc
     8101 aattgcgctt ccaggtcttt aacatcagca ttctgatagc ggaatcgttt ggctttgcaa
     8161 agtcttacac catcaatgat tgatgcatga ttgagggaat cggagatgat agcatcttcc
     8221 tctccaagca ggggttcaaa aacgcctcca ttagcatcga agcaagctgc atataaaata
     8281 gtgtcatcgg tgccaaaaaa ttcagctatt ttccgctcaa gagtcttatg caaatcagta
     8341 gttccacaaa taaaacgtac cgacgacata ccatatccat gcgtatcaag cgcctcttta
     8401 gctgcttgta tcagtgcagg atggttggat aatccaagat aattgttggc acaaaagttt
     8461 aatactgtct gtccgctgtc aagttttatt tcggcctgtt gtggcgaaac gattattctt
     8521 tcatgtttaa ataatccagc ctcctgaata gcagccagtt ctttctgaag atgatcttta
     8581 atttttccgt acattgtaac gtgatttaaa atgtttaagt tttacttgtt attcaaataa
     8641 tagactgcaa aaatagtgat ttctttagtt tcggtatttg atctttgcag tttttggata
     8701 attcttggat cagcccagat gttttaataa tcaggattag acttctaaac tcaatagtta
     8761 ggccctaaaa ttttttctat taatatattc ggatctaaat gacaaaaaaa ataatctttt
     8821 accttttttc agtacttttg cgacataatc ttttttctta gaataagcta atcaactgaa
     8881 gctgtgaaca atttaacatc aaaaaaaata tcgagcgcat taatatccgt ttatgacaaa
     8941 accggtatta ataccattgt taaagcttta ttaaaaaatg atgttgaaat aatctctact
     9001 ggcggaacat acgattacct gactaaaaaa ggtttttcat gtaaaaaagt tgaagagatt
     9061 accacctatc cctctatttt aggcggtaga gttaaaacat tgcatccact tatttttggc
     9121 ggtattttat cgcgacgcga caatcaggac gaccagcatg aattggaaca atacaaaata
     9181 cctacgattg atctcgtaat cgttgacctt tacccttttg aaaccaccct aattaacact
     9241 caaatagagc aaaagatcaa cgaaatgatt gatattggag gaatcagtct tataagggca
     9301 gcagctaaaa attataaaga tgtggttgtg gtaccatctg taaaatatta ctcccatttt
     9361 cttgagattt attcaaacaa cgatggtaga tttactattg aagagcgaaa attttttgct
     9421 gcctgtgctt tcaatatctc ttcacattac gacactgcta tttttaactg gttcaatcgc
     9481 gaggtaaagc tgcaggtatt caaacagagt attaccgatg taagtcatct ccgttacggt
     9541 gaaaatcctc atcaggaagg agtctatttt ggcaatatta ccgaactttt tgatcaattg
     9601 aacggaaaag ccatttccta caacaatctg aatgatattg aagctgctat ccagcttgcc
     9661 aacgaatttg acgaagtttt ggtggctatt atcaagcata ataatgcctg tgggcttgct
     9721 tgccggcaat cattgaccga agcatgggga gctgcattag aaagcgatcc actatcagca
     9781 ttcggaggcg ttattattac aaatagagca gttgattacc atacagcatt agaaataaat
     9841 aagttgtttt tcgaagttat cattgcccca gattttgagc aaaaatcctt aaaacttctc
     9901 aaacaaaaga aaaaccgaat catcttgcgt caaaaagttt ttgcgctgcc aaagttacaa
     9961 ttcaaatctg cgctaaacgg tgtactggtt caggataaag acttagttga agaggacaag
    10021 ataaagttag atttcgtaac caagagcaaa gcctcatccg aagagattga agatcttttg
    10081 tttgccaaca aaattgtaaa acacacgaga tctaatgcca tagtgttggc taaaaacaaa
    10141 cagctccttg gtagcggtat cggccagaca tcgcgcattg atgctgttaa acatgctata
    10201 gaaaaggcaa aatcgcatgg tttcgatttg cagggagcag taatggcttc cgatgctttt
    10261 tttccttttt ccgattcggt agaaatggca tataaagaag ggatcagagc gattatacaa
    10321 ccgggaggat cgcttcgtga tgccgactca ataaactatt gcgaccaaac aggcatgtca
    10381 atggcattta ccggaatacg acattttaaa cattaaatga gtagatttgc attcgtttta
    10441 tctaaagtaa ctaattgaca aatttcataa tcagagtatg ggtttatttt catttttcac
    10501 aaaagaaatt gcaattgatc ttggcacagc caatacaatt ataatataca acgacaaagt
    10561 agttgttgac gaaccttcta ttgttgccat tgagcgtagt tcagacagaa tcattgccat
    10621 agggaagcgg gcgcagatga tgcacggcaa aactcacgaa aacatcaaga ccatacggcc
    10681 tttgcgcgat ggtgttattg ccaactttca ggctgccgaa tacatgattc gtgagatgat
    10741 taaaatgata ggtattagag gttctatctt tccaccagcg cttaaaatgg tgatttgtat
    10801 cccatccggg attaccgaag ttgaagagcg cgccgtaaaa gattcagccg agcaggccgg
    10861 cgcaaaagag gtaaaactga tacacgaacc tatggcggct gcaataggta taggtattga
    10921 cgtattagag cctaccggaa acatgatcat tgacattgga ggaggcacat gcgaaattgc
    10981 ggtaatcacc ttaggcggca ttgtaaacaa caaatcaata cgcgtagccg gcgatgactt
    11041 caacgccgat attgaagact atatgcgtaa acaacataac ataaacattg gagaacgtac
    11101 tgccgaacgc ataaaaattg aagtcggcgc tgcacttcca gaacttgaca acccgcccga
    11161 tgactatgcc gttcatggtc gagatatgct tactggcata cccaaagaga tcattgtcaa
    11221 ttataccgag atagcacatt gccttgataa atccatctta aaaattgaaa cggcagtgat
    11281 gcaagctctc gagatgacac caccggaatt gtcagcagac attttccgta cgggggtata
    11341 tcttgccggc ggcggctcaa tgctcagagg cttcgacaag cgcttacacc ttaaaacaaa
    11401 actccctatt catgtagccg aagacccatt gagagccgtt gcacgcggta ccggtattgc
    11461 attgaaaaat tacgataagt ttactttcct tattaagtaa acctgttttt gatatccctg
    11521 taaagatgca aaatctattc gcatttatct ggaagaattc attttatttc ctttttgttt
    11581 tgctcttggt tctctcggtc gtactcatgg ccaggaataa ctactatcat cgaacggtca
    11641 tgataaatac aaccaatgaa ataacgggtg gtatattatt ggttgcaagt aatttttcgg
    11701 attacatgaa actaaaaaga acaaacaggg aactcgccga agaaaacgca aagttaattt
    11761 ctcaaacaaa ggagctattc ttagacgcct caacgaaagc tattccctct cccgacagca
    11821 atgtattaat accacaatac gaatttattg ccgccaaagt aataagtaat tcggtaaatc
    11881 ggcgcaacaa ctacataaag ttagacaaag gcagatttca gggtataatg ccaaatatgg
    11941 ctgtaatttc acctgccgga attgttgggc atatagtaga agtatcgaat cattttagtt
    12001 cggtgatgtc ggtgcttaac cgtaactcaa ggattagcgc caaacataaa acctcaaatc
    12061 atgtcggctc ccttctttgg gatggaatag attatcgctt tgggaaattg gtagatttac
    12121 catcgcatgt taagatattt cagggggaca ctatagttac cagcgggtat tctcatttat
    12181 atcccgaagg agttattata ggtacggttc aagaagcaat acttgaatca ggcgccagct
    12241 tttataacgt aactgtctct ttttcaacag attttaatag gttatcgtat gtatatgttg
    12301 taaataatct cttttacaac gaattggaca gcctacaaca gttgcaaccc caaaatgagc
    12361 ctttgcgatg attatacaaa atgtaatacg atttatcgct gccttgttat tgcaagggct
    12421 tatatttaat cagataggaa tcggagccta cctcttccca atgttttatg tttattatat
    12481 cctaatgtta ccattcgaaa ccaaagggtg ggttttactg attacctctt ttttactcgg
    12541 tctgggtgtt gatttttttt ctaattctct tggcttacat tcaacggcat ccctttgtat
    12601 ggcatttttt cgcccggggg tcatcaggct cttgactgta ggtaaagatt atgaaacact
    12661 tgtttaccca agcatcaaaa aatttggctt ttcatggttt ttcagttata cagtaattct
    12721 tgtcattatt catcatacga tattttttat ccttgaagta ttcagctttt acgaaatcag
    12781 gcagattatt ttcaggatat taataagttc tatagctact atatttttaa tctttataac
    12841 gcaactactt tttttaaaaa gagaaaaaca ttaaaaatca actaattcac aattaatcac
    12901 taatagataa tatcaattat gaaaaaatca tttttagcaa ttattcttgc aatgttttta
    12961 ttctcgtgca gcactcctga gaatcagttc gaaatcatcg gtaatctgcc cgaagcctcc
    13021 gacaactggg tagtactggc caaagccgtt gacaatgatc ttaagactat tgattcggta
    13081 atggtaaaag ggggaaaatt ctactttaaa gggatcattg attttcctga gatgtattac
    13141 ctctatttca aagctgacga caaatattta ggttttttta tcgaaccggg taaaatgaat
    13201 ataagtggta ctttcgatga gccaatatat gatgggttgc ccatacaggc gttatacgat
    13261 aaattaatgc acgacctgaa gtcatgggaa ggtcaatttg aaattttatc agctcaatat
    13321 cgcgaagctt cagcgaaagg cgacttagat atgcaaaaag ccattgagga gaaagctgaa
    13381 ttaattgaaa ctgagagaaa tgaacatata atgaaattca ttgaagagaa tagcaacagc
    13441 gtgattgctc catttttgct ttacaacaac attcacagct ttgatataga aaaagtggag
    13501 gctattacaa atgtcattga ctcctcagta cacaagtcgg tttattacac cttacttaac
    13561 gatgagttgg aaaagctatt aaaggtatca attggtcgga tggcgcctct cttttctcaa
    13621 aacgaccctg atgggaatcc agtagcgctc gaatcgttta gagggaagta cctgcttatt
    13681 gatttctggg catcatggtg tcagccttgc cgattagaaa atccgaattt ggtagccaac
    13741 tatgccaaat atcgcgcaag agggtttgaa atttttggag tatccttaga tcgcgataga
    13801 gctgcatggc tcaaaggtat cgccgacgat aacctcacat ggccacaggt atccgacttg
    13861 caattctgga acaatgaagc ctcaaggctt tatggcgtaa actcaattcc tgccaacttc
    13921 ctcctcgacc ccgatggaat tattatagct aaaggccttc gtggcaaaca attggaagat
    13981 aagctggcag agatatttcc atagaaaaca ttaaatcatc gtacagagct aattcagttt
    14041 gttacagtca tgatttttgg ttttagcaac gaccgattat aaatcagtat taagtagggt
    14101 ttggtgaaaa agattttggc agtcaatcgg aaattacatt gaagaaaaat tggcaaggaa
    14161 aaaatgctta agatttaaac aggatacgca ctcacacaac gagaaatagt gagaattcga
    14221 tcgcttttat tatgtttatg atgaatctta ttaagttcat taaaattttt tgttttcttt
    14281 tacgctattg ttcaaaaaca ggtatggcaa gttattttta tgcctgcagc aggtcgagaa
    14341 aaaatgaatc tcatcacaat aaatcctgtt ttattcacca aaccctactt atttatttgt
    14401 aattcactac tataagcatg aggctttcgc cttcatttac aaagaataat ttcaaattat
    14461 taatcgtttt tagtttcgta tctctaaata tgtattttta caaccacaat ttctattaat
    14521 caaaataaaa aaaacattta tgaaaagtat aaaaggaact caaacagaaa aaaatctgtt
    14581 gaaatcattt gccggagagt ctcaagcaag aatgcgttac acctattttg caaaagaggc
    14641 tgataaagaa ggttataaac aaattgcagc tattttcatg gaaaccgccg agcaagaaaa
    14701 agaacacgca aaacgcatgt ttaaatattt agaaggcgga atgctcgaaa ttacttcaac
    14761 ttatccggca ggagttatcg gaactacagc cgaaaactta gaagcagctg ctgcaggaga
    14821 aaacgaagag tggacaaaac tatatcctca ctttgccgat gttgccgaag aagaaggatt
    14881 taaagagatc gccacaatgt acagaatgat agcgaaagcc gaagcaattc acgaagaacg
    14941 ctacttgaaa ctgttgcaac gggtgaaaga tgaaaccgta ttctcacgag acgaagagat
    15001 tgagtggcaa tgtcgcaatt gcggatatgt tcataaaggc aaaaaagcac tgaaaacatg
    15061 tcccgcttgc ttacattcgc aagcctattt cgagcctaaa tgcgacaaat actattagtt
    15121 aaagggtaat tggcgaaaat caaaagcagt taactaattg aatatctaaa cattgtagag
    15181 atggagcatg ctccgtctct ttcgttttca gaaactttta actatcatca cacattcact
    15241 ttctcccccc caccccaaat tcaattgtta actgctaatt gctaattgta ttatcttgtg
    15301 cttaaagcga tacacacttg ttgcattttg cgtgttgcaa attaaaacaa acaaaatgac
    15361 caaaaaactc aactctaaaa acagtctgct cggcaaatct gcatctatta tctctgtgat
    15421 actaaaggga tttggacaaa tcatgcttca ggaaaatagc attacgggtt tgctgtttct
    15481 gattgggatt ttctacggtt cacttgctat gggttttgcc gcacttttag ctaccgtatg
    15541 cggaacagct accgcctatc tgctaaaata cgacagaacg gaaatcaaaa aaggactcta
    15601 cgggtttagc gccgcattgg tcggagttgc agtactgctt tttctaaaac ctctgttttg
    15661 ggcttggatc attgtcggtg ttggttcggt gttggcaact atgttgcagc acttttttat
    15721 aaaacgtaaa atccctgcgt tcaccttccc gtttgtttta attacttggt tgatactctt
    15781 tatctgcaac aatttttaca gcgatttatt ggcaatacct gtgcctacta tcgcccattt
    15841 gagcgactct ctatcagacg gtttcaagag cttcggacag gtaatttttc aaaacaaatt
    15901 agtgtcggga ctgctttttt ttatagccgt atttatcagc tcgcccattg cggcacttta
    15961 cggacttgca ggagcaattg tttccgacat tattgctttc gagttttccg cacctataga
    16021 taatatcaac cttgggcttt atggttttaa cgccgttttg tgtgcgatag tttttgctgg
    16081 aaatcaagtt aaagacggca tttgggcttt tttatcggtt gtactttccc ttggcattag
    16141 tctattaatg ataaagttcg gtcttacgca acttacattt ccgtttgttt ttgcaacttg
    16201 gattacccta tttctgaaaa acagactaca aaaaccaata aacgctgtga tattcaaaga
    16261 tagaacaaag ccaaccaatt cacagtaaat tagttggctc tgttttgtta acttctaaat
    16321 atctctaatt agtcgttttt cgtctattat actttgcaat ttactatttc gggaaaccta
    16381 cggtttgcgc taataaaacc tcatgagatt taggcaattg tagtaagcta tgaagttctt
    16441 ccttattgaa agcgcctctc gcaacagttg ccaatccctc tgaggcgcaa tagagataaa
    16501 tgttttgcga aatataccca cagtttgtgt aggagtattg tgaacttgcc gctttttccg
    16561 aatcgctaac aatcaaaata ttaagcggtg catcggctac ataatcttgt attccagcgc
    16621 tttttcgatg atcgccatct tttactaatg tgagcttatg ctcctttgca ttgtaaaaat
    16681 agattccctt ttttaaaatt acataaagct ctaactcctg acaatttcta gctgtgggag
    16741 ccgttcgctt gtcttctcta ttaaagccgt tggcagccca taataaatca gataattgtt
    16801 ggtcggagag atctttctcc gaaaacgagc gagctgattt tcgttcattt aatgatttca
    16861 tcaaaggttg tccgccactc ctatcaggag ctggtaattt aatttcctgt gcattcatac
    16921 tatatcctaa acagattaaa aaaataaata aaatttttgt tttcataagt taattaattt
    16981 aatggtttaa tatcataaat aattcacata cctcataaat tatcccccac agtctaaacc
    17041 gagagctaaa atgcgtggta gcgttttttt taatgttgct atttggtgtt tctttgatgt
    17101 attttttact tgccttcaat attgcaattt caaaaaccat ctcttatatt ttccagttta
    17161 tctaattgtt cttttaattg ctgtttcgtt tgcgtctctt tttctgaatt ataagcggct
    17221 ctcaagtccg gcagataatg tatgtaatgt gtatcggcga tatacttagc agcacgaaca
    17281 cgaacagaag cgtcagcatc gaacaataac ccctgaaccc aacgttttgc agcccatgaa
    17341 tgttttgatt gcaaccaatc cattgctacg attttctctt ctgccgaacc gtataaaaat
    17401 gtctgataat atgcagattc ctgttttaag tcgctaatac tcatcaggat ttctcggtta
    17461 aaaatatcag ggtttactac ttccgactta tattctttta tcggtaaatt tagtgtccaa
    17521 cgcaccatac gtggaatcat ccacatcatt ccaggtgttg cttcgggatg tgcaattgaa
    17581 ctaaaaatcc ggccttttcc ataggagtta gtaataaaaa acggcttgtt gttagtcata
    17641 tttttcgggg cgtttccttc ttcatgaaca tcactttcca taatagcaag cgtttcatac
    17701 gaaattgtat cgttttcctt ttttataaaa actggacctt cgtaatacat aacgaacaga
    17761 gtatcgcgct ttgcaatctc gggaaatatc cttttacctt catctgtaag tgtaaatttt
    17821 gatattccgt gtccacgatt atcatgttca atatctattg cttgagcccc gtttagttgc
    17881 atgcaggcat aatccggtgt atttgaaaga agataagctc cagcacaaat tcccacgatt
    17941 ccacctccag aagcaacaaa atctttaatt cgttgctgat ttaatgcact caggtttaaa
    18001 aactgacgac ttcctccgcc tccgggtatg ataatggcat caagcgaatc gagtacatta
    18061 ttagcaatat cggcagttgt aatggtgcga acactcatgt ccgtatctaa cttaacagct
    18121 gctactgttt cccaaataca ggtttgcgct ccgccatgcc catcgaaaac agcaacttga
    18181 atcttattgt taagttcttg atctgttgtt tgtttttgtg tacattgtac aaagaataac
    18241 aaaaaaatga taggaagtaa taatttcata attagtgttt atattaatga tgatctatgt
    18301 gtaaatttca aaaaaattga aaaacctatt gttaacttta ctaaaattat tcaattttat
    18361 tcgatttctt tttttaccgt aagttttggt tgattgttta ttgttgcttg tctaaagttt
    18421 tataatccac actattttag ggaattatcg cagtttctaa gagttttatt tacaattgca
    18481 aatgtactgt tttctcataa gttagcaaaa ctgttttttt taaatatcta ttttatcagg
    18541 catcagttaa tttttgtttt ctcttatcaa caacagtaaa tatgatcatt agaatggtag
    18601 caataaatgc cattacagag ccaaaataaa aaggagcatt tgcattgacc ctatcataaa
    18661 gtaacccagc gattaaactc gcaggcaata aggtaattcc cagcacagcg tgataaatac
    18721 caaaccccgt gcctttcaat tctttactta caatatcaga aatcattgct ttctggctac
    18781 catcagtcaa tgcactgtaa aatc
//
LOCUS       DAOOAI010000037        18574 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_3956_length_18574_cov_5.358011, whole genome shotgun sequence.
ACCESSION   DAOOAI010000037 DAOOAI010000000
VERSION     DAOOAI010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18574
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3956_length_18574_cov_5.358011"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1537)
                     /locus_tag="PK345_04075"
     CDS             complement(<1..1537)
                     /locus_tag="PK345_04075"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPX34233.1"
                     /translation="MQKINLNNFEQFSSSLKKNILPDVPMVMVGMGTCGIGNGADALY
                     ETLQKHAAQRKYDCIIKPTGCFGFCAEEPLVTLYQPGKPLLVYARVDTKDAIKILDGG
                     MSGNIYKRKVLCRIENWDFITSKFQYGTGYEKYPLWNEIPFFKGQKKIVLRNAGLINP
                     ESIEDYLAVGGYRALFKVLSGMTPDDIIAEVKNSGMRGRGGAGFPTGVKWELMKKNHS
                     EKKYLICNADEGDPGAYMNRNEIESDPHSILEGMMIAAYAMGSDEGIAYIRAEYPLAV
                     KRLEKAQMQAEALGLIGNKILGTDFSFKFDFVEGAGAFVCGEETALIASIEGKAGRPV
                     PRPPYPSAKGLYGHPTNINNVETWCNIPVIINRGAQWFRETGTEKSPGTKVFSLVGKI
                     KNTGLVEMPMGSTINQFVFDIGEGTGSTVKRVKAIQTGGPSGGCIPLELFNTAVDYES
                     LAKIGAIMGSGGMVVMDQDNCMVDVARYFLEFNSAESCGKCTPCREGLTQALRILTKI
                     TRGQ"
     gene            complement(1515..2048)
                     /locus_tag="PK345_04080"
     CDS             complement(1515..2048)
                     /locus_tag="PK345_04080"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPX34234.1"
                     /translation="MDTLTPYEVEEKVLDEIVGKYKGSPGVLLSTLEAVQEANPLKFL
                     PQKTLLQVAKKLNVPATQVYSVATFYSFFNLKPQGKHTIVVCRGTACHTKGSRELLNS
                     VGQILGFRYEFDETEPSYTTPDNEFTIRTVACFGQCAQAPVVAVDGVIHSNVNAPKLL
                     KILKKVKSSSYAKNQSK"
     assembly_gap    2543..2552
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2831..3295
                     /gene="mraZ"
                     /locus_tag="PK345_04085"
     CDS             2831..3295
                     /gene="mraZ"
                     /locus_tag="PK345_04085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HPX34235.1"
                     /translation="MFTSTYECKIDDKGRLALPGKLKAELMPVLADGFILKRSVFSPC
                     LELWPKKEWDKTLTRINALTRFSRENAKMVRAFLAGVKQVDLDGTGRINIPNDLIIFA
                     GIQKTVVIASQISIIEIWDKSKYEEEVSIANDVEFAQMVENILGGLEPDNTI"
     gene            3298..4191
                     /gene="rsmH"
                     /locus_tag="PK345_04090"
     CDS             3298..4191
                     /gene="rsmH"
                     /locus_tag="PK345_04090"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HPX34236.1"
                     /translation="MYHQPVMLKESIQGLNIKPNGIYVDATYGGGGHSAAILQGMPKG
                     RLIAFDQDKDALVNRINDVRLTLVNHNFRYLKQFLKYYNSLPADGIIADLGVSSHQLD
                     SKERGFSTRFGGDLDLRMNQNQKVNAQYIVNEYNEEQLAFLFKNYGELSAYSAIAHEI
                     VKERASNKIKLFEDLKRTIEHLAPKKEENKFFAQIMQALRIEVNQEIDSLKELLQQAV
                     EVLRQGGRLVVISYHSLEDRIVKNFIKTGNFEGVVEKDFFGNKKLVFKSITSKPIVPS
                     AEEIKQNSRSRSAKLRIAEKL"
     gene            4202..4582
                     /locus_tag="PK345_04095"
     CDS             4202..4582
                     /locus_tag="PK345_04095"
                     /inference="COORDINATES: protein motif:HMM:NF039650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsL-like putative cell division protein"
                     /protein_id="HPX34237.1"
                     /translation="MTRNANILKSEQKQIASKKKKRTISEHVHSFLDGSFIAQGKIRG
                     RILFLLFLVLLGLGYIRNNYLVERTIRKLNKLNNELVELHYDYIQMKSTVNLRTQPSV
                     LEEELQAYNIKRLERPPIKIFLQP"
     gene            4594..6717
                     /locus_tag="PK345_04100"
     CDS             4594..6717
                     /locus_tag="PK345_04100"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HPX34238.1"
                     /translation="MKENNKTTKKERAGLDKGVMSRIYIVYVFLLLIAIGIFLRIVQL
                     QIFKANELNIIAEKREFRFDDNIEAMRGSIYSEDGRLMATSVPVFEVRMDVALPDIKD
                     SVFNDSVTWLALGLSKMFGDYTNWEYKQRLIKARNEKNRFFLIQKNVTFDQLTQLKTL
                     PLLKRTRDYNSLIVIRTPRREYPFGILARRSLGYFKDHPTDSLKVLVGVEGEYNEYLQ
                     GIKGRQLKQRMAYGEWKPIDHLNNIEPVNGKDIVVTINSYLQDIAHSSLKNQLIAHNA
                     DKGCLILMEVETGELRALVNLMKDTKDGEFKESYNMAVGELFEPGSIFKLPAMMVAIE
                     DGVVQKIDSVYIGNGEVKYNNRVMKDSHRFDPDGWVTPENCLAHSSNVGVSKIIYDHY
                     NGNNKSFYNKLTKMFSADITGVDIPGEPKPFIKDPHLKDKRNYWSSVTLPWMSIGYEV
                     LTTPLQMLTFYNAVANNGKMVKPRIVKEVRENGNTVKVFESEIINPSICSQSTVALAK
                     HFLLSTVENGTGRNVKNNDYKIAGKTGTAKINENGRYISKYNASFIGYFPADKPKFSC
                     IVVIYRPNEGAYYASQVAAPVFKEVADMVFAFAYNIDPLNKNQFIAHKDENGAPMNRL
                     PDQVNPQLAELVNYEGVKPLEESKCPMVIPDVTGLSVKDATHLLEACNLVVKIHGKGL
                     VKIQSPEPGTPYKAGDMVELKLEII"
     gene            6722..8197
                     /locus_tag="PK345_04105"
     CDS             6722..8197
                     /locus_tag="PK345_04105"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HPX34239.1"
                     /translation="MMVRQLNKILESIDFLQRVGTTDSILRNISFDSRTVGHDDLFVA
                     VKGVKMDGHDFIPEVINKGVAAVICERIPDNMPENIVFIKVRNSAVALGQAASAWYDH
                     PSTKLKVIGITGTNGKTSTVFLLYHLFKGLGFKTGLLSTIENRIGDEKVASTHTTADS
                     IQINKNLAKMIESGCHYCFMELSSHAIDQERIAGLKISGAIFSNISHDHLDYHVTLDN
                     YIKAKKKLFDELPTDAFVLVNADDSHSKIMLQNCVARKFNYSLKSPSDYKGRILENSF
                     DGLLLRIDNQEVWCRLVGGFNAYNLLATFAVARIEGFQNHEVLPLLSLLAPVEGRFQI
                     IRSKAGITVIVDYAHTPDALKNVLKTINEVRSGKEKLITIIGAGGDRDRLKRPLLAKI
                     ACRLSDRVILTSDNPRTEKPEMIIDDMEAGLDRDMKKKTIRITDRLEAIKTAIMISLK
                     DDIILIAGKGHETYQEVNGVKSHFDDREIAVEYLNNLNSDN"
     gene            8197..9453
                     /gene="mraY"
                     /locus_tag="PK345_04110"
     CDS             8197..9453
                     /gene="mraY"
                     /locus_tag="PK345_04110"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HPX34240.1"
                     /translation="MLYYFFGYLDKVFDFPGARMFQYISFRAGMAVIVSLIISMILGK
                     SLINLLKKMQVKESIRDLGLEGQKEKEGTPTMGGLIILGAILIPTLLFADLTNIYIII
                     ILITTIWLGFIGFIDDYIMVFKKDKKGLAAKFKILGQVTLGLFVGLMMYYHPDIVIRE
                     RTSDISFIESIEKVETDDLRDVANDLYSKKNIKSTKSTIPFFKNNELDYSIFVSWLGK
                     DYQRWTIIVFVAVVILIITLISNSANLTDGIDGLATGVSAIIGSTLAVLAYVSGNIFF
                     ANYLNIMYLPDSGELVIFIAAFVGACIGFLWYNTFPAQVFMGDTGSLALGGIIAVFAI
                     MLRKELLIPLLCGIFLAESASVVMQVSYFKYTKRKYGAGKRIFKMSPLHHHYQLKGYA
                     EPKIVQRFFIVGIMLAVFTVITLKIR"
     gene            9462..10814
                     /gene="murD"
                     /locus_tag="PK345_04115"
     CDS             9462..10814
                     /gene="murD"
                     /locus_tag="PK345_04115"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPX34241.1"
                     /translation="MSNSIAILGAGESGTGAAVLATKKGHRVFVSDKGLIENKYKEVL
                     SQYRIEFEEGKHTEELIFAADEIIKSPGIPDEIDLIRRVKALSKPVISELEFAGRYTR
                     AKKLCVTGSNGKTTTTLLLYHILKNAGLDVGLCGNVGKSMAMLLAERDYDYLVIEVSS
                     FQLDGMFDFKADMAILTNITPDHLDRYDGKFENYAASKFRIIKNQRDTDYFIYCNDDP
                     EILNRLDIYKPSASCYPFSIAGDIKREGAFLDSKHQPNKMYFNIKSDIMIMTLEELAL
                     KGMHNAYNTMAAGIAARLLDIRKESIRNSLADFQGIEHRLEFVATIRGVDFVNDSKAT
                     NVNSAWWALDNGYKPIIWIAGGIDKGNDYSEIADLVAKKVKAIICIGVNNDKIIRQLG
                     RYVDVCVEAASMQEAVEMAYQLGTKGDQVLLSPACASFDRFNNYEDRGNQFKKAVINL
                     "
     gene            10826..12004
                     /locus_tag="PK345_04120"
     CDS             10826..12004
                     /locus_tag="PK345_04120"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HPX34242.1"
                     /translation="MNKTIIKGDKVIWMITLFFAITSLLVVYSSTETLAFQNKTTTER
                     LLLKQAIVMLCGIGLLYFAHTLKYVYYSRISQFLMILAGAFLLMTFFVAPEINNAQRS
                     LRFTLPIVGQISFQPSDLAKLAMMIFIARFLSKNNTQKMGFKEMFLKIGLPLFIITLL
                     IIRSNFSTAFLLFGVSIVFLFIGKVKIKHLSYLILIAVAGLGMIILIGLIKPEVFPRA
                     KTWVNRVSAFSMSSEKEEPNVQIVHSEIAIAQGGLFGKMPGKSTQRIFLPLAFSDYVY
                     AIIIEEYGLVGGFTVLLLFTALLFRSIKIARRCQMKFGGFLVVGISLMLVCQALFNML
                     VAVNLAPVTGQPLPFLSMGGTSFWFSCISIGIILSVSRSLELDYKQQYSEDLLANGSE
                     "
     gene            11991..13124
                     /gene="murG"
                     /locus_tag="PK345_04125"
     CDS             11991..13124
                     /gene="murG"
                     /locus_tag="PK345_04125"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004324393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HPX34243.1"
                     /translation="MVANELKILISGGGTGGHIFPAIAIADALRNIVPDCDILFVGAL
                     GKMEMEKVPQAGYPIKGLRISAFQRRLSLKNLSFPFKVVFSMVKAYKIITSFKPDVVV
                     GVGGFASGPTLKVAVNRRIPVVIQEQNSYPGITNRLLAKHAERICVAYDGMEKWFPAG
                     KILKTGNPVRENVINIQNKKDEAFRYFELESGKPVVLIVGGSQGALSINQALSYNLKD
                     LEQCGVQMIWQTGKLFADKAKSAIQEKIANPRDKDFHVYEFITRMDLAYAAADLVISR
                     AGAIAISELSAVQKPSILIPYPAAAEDHQAKNAKALADKQAAIFLADSEAKIKIATIV
                     KSLIEDEEKRDTLSKNIGEFAVHNSAQKIAEQIIKIASKRNGV"
     gene            13114..14511
                     /gene="murC"
                     /locus_tag="PK345_04130"
     CDS             13114..14511
                     /gene="murC"
                     /locus_tag="PK345_04130"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HPX34244.1"
                     /translation="MEFKEYSHIFMLGIGGIGMSALARYFKLKGKFVAGFDRVNTQLT
                     QTLENEGFTILYKDNVDEIPYDFSIPEKTLVIYTPAIKNDNTLLTYFSQRQFKVFKRA
                     EILGRIALEYKTIAVAGTHGKTTITAMVSHLLHQSAVEVNAMVGGIMKNYQSNFIASA
                     KSEFLVTEADEYDRSFLKLYPHFAVITACDADHLDIYGTEEELLKAYGKFADQVSAEG
                     LLLTKKGLQLNVQNASVTRKYTYSVNDRSADIYPDHLQFNENMISFDLVTPQGVLEKL
                     TMQPTGLMNVENAVAATFIALQAGADRDEIKEGLSTFKGVRRRFDMLYNDNETIYIDD
                     YAHHPQEIRSLVKSVKSIYPSRHITGIFQPHLFSRTLNFADEFARSLEQLDTIILLDI
                     YPAREKPIEGVNSAFLFKKINHNNKFLFARDETIEYLLHHHFEVLLTIGAGDIDTLAQ
                     PLLKMIRHKKVIKEK"
     gene            14511..15305
                     /locus_tag="PK345_04135"
     CDS             14511..15305
                     /locus_tag="PK345_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34245.1"
                     /translation="MKKILQISFWLIILAGIIVIFGFAVSEQKKTVCSGLVINLKDAE
                     QPGFIYEKDVRAIISQLYPSLRGKILDSLNTAAITKELNRSPYLKNPIVVKTLNGLIK
                     VQLERTEALVRVVNSKGDSFYLSKEGYVMPISRSYIPKTLIVTGHIYDSPNPYDKLIY
                     NVNGGTASKKSILNKIFFLAETIINHSVFNQSIDQIYVTDCNEFEMVPSSGDHIILLG
                     DVENLNHKLENLNAFYQAGISKLEEGEYKIFDLRFKDQVVCKKLMP"
     gene            15302..16603
                     /gene="ftsA"
                     /locus_tag="PK345_04140"
     CDS             15302..16603
                     /gene="ftsA"
                     /locus_tag="PK345_04140"
                     /inference="COORDINATES: protein motif:HMM:TIGR01174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HPX34246.1"
                     /translation="MNTANIVVGLDIGTTKIAAIVGRRSEYRKIEILGYGRTESIGVK
                     RGIVSNIELTVDSIKRAIQEAEAKSEVDIKYVNVGIAGQLIKSLLHRGGLVRKNIEVE
                     ISQEEIDNLLNSMFNLNMNPGEEIIDVIPQEYIIDNEPDIREPIGMLGSSLEANFHII
                     IGQTTAAKNIYKCITKAGLEVVDLILEPIASACAVLSEEEKEAGVALVDIGGGTTDIA
                     IFQDGIIRHTAVIPFGGDVLTEDVKEGCSIIRKHAEELKIKFGSALANENKDEEIVAI
                     PGLRGRPPKEISLKNLASIIQARMEEIIEHIYYEIKNSGYEKKLIAGIVLTGGGSQLR
                     HLAQLTEFMTGMDTRIGYPNEHLAGNAPDEMHSPMHATGIGLVLEGVARLEKQLERDS
                     EINDDNGRSGKAKISEKSEKKGKRPIGFLRKIQEFFEDGQE"
     gene            16634..18277
                     /gene="ftsZ"
                     /locus_tag="PK345_04145"
     CDS             16634..18277
                     /gene="ftsZ"
                     /locus_tag="PK345_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HPX34247.1"
                     /translation="MNDMLLFDAPKQRASIIKVIGVGGGGSNAVTHMYKQGIKGVDFV
                     ICNTDSQAMDISAVPNKIQLGKTGLGAGSIPDVGRLSAEENIEEIKKIIESNTKMLFV
                     TAGMGGGTGTGAAPVIAKLAKEMGILTVGIVTLPFNFEGRKRRLQAEKGIEELKKYVD
                     TLLLISNEKLREIHGNLKISEAFGKADDILTTAAKGIAELITVAGYINVDFEDVKTVM
                     QNSGTAIMGSASAEGNDRARIAVKEALTSPLLNDNKIVGASNILLYISSGKEEITFDE
                     VTEITDYIQEEAGENAEIIWGNGYDDSLGDKISITLIATGFLSPEEIDENTLNRQKKK
                     RVYNLNDDLPNKQSNEQAELETLTIVETDNKQDIECKPIEITEITLLKSESVDNKKVA
                     NPTREINLGLFENIENKIQSKSSDQSSSQQVPVRPFNDGPLKNSSLKIKHTVGDPIPF
                     NDLSSKREELAQQKSVIDKKADDRVEKLRSLSIFGDKFADKIDEMEKVPAYMRRNVQL
                     NPVTPSSESAITRYTLSENEDFDAEIKSNEIPFLHNKPD"
     gene            18327..>18574
                     /locus_tag="PK345_04150"
     CDS             18327..>18574
                     /locus_tag="PK345_04150"
                     /inference="COORDINATES: protein motif:HMM:NF020977.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HPX34248.1"
                     /translation="MSLVEIINEDIKNAMLSKDKRKLEALRAIKAALLLIKTGKDKSS
                     DEIPETLEMQMLQKLVKQRRETAVIYQQQNRQELADEE"
BASE COUNT         6031 a         3118 c         3942 g         5473 t
ORIGIN      
        1 cctgtccgcg cgtaatcttg gttagtattc tcaaagcctg tgtcaagcct tcgcggcaag
       61 gtgtacactt tccacaggat tcggcagaat taaactccaa aaaataacga gcaacgtcaa
      121 ccatacagtt gtcctgatcc attactacca taccacccga acccatgata gcgccaatct
      181 tggctaagga ttcataatca actgcagtat taaataattc caaaggaatg catccgcctg
      241 aaggacctcc tgtttgaatg gctttgactc ttttaacggt gctgcccgta ccttcaccta
      301 tatcaaatac aaattgattg atagtagaac ccatgggcat ttctaccaat ccggtatttt
      361 taatttttcc tactaatgag aatactttgg tgcctgggct cttttctgtt cctgtttcgc
      421 ggaaccactg tgctcccctg ttgatgataa ccggaatatt gcaccaggtt tccacattat
      481 tgatattggt aggatgaccg tataagcctt tagcagaggg gtaaggaggg cgcggaacgg
      541 gacggccagc ttttccttct attgaggcga ttaaagccgt ttcttcccca caaacgaaag
      601 cgcctgcgcc ttctacaaaa tcaaacttaa aactaaaatc cgttcctaat atcttgttac
      661 ctatcaaacc taaagcttca gcttgcattt gagctttttc caatctcttg actgccaaag
      721 gatattcagc acgaatatag gcaatacctt cgtcggaccc catggcgtaa gcggctatca
      781 tcataccttc taagatggaa tgaggatcgc tttcaatttc attgcggttc atgtaagctc
      841 ccgggtcgcc ctcatcggca ttacaaataa gatatttttt ttcactatgg tttttcttca
      901 tcaactccca tttcacaccg gtagggaagc ccgcgccacc acgaccacgc atgcctgaat
      961 ttttcacttc agcgatgatg tcatcaggtg tcatgcctga aagcactttg aataatgcac
     1021 ggtatccacc tacagccaag taatcttcaa tggattcagg atttatcaaa cctgcattgc
     1081 gcaaaacgat ttttttctga cctttgaaga aaggaatttc attccacaaa ggatatttct
     1141 cgtaacctgt gccatattgg aacttagaag taataaaatc ccaattctca atgcgacaca
     1201 atactttgcg cttgtatata ttaccagaca taccgccatc taaaatctta attgcatctt
     1261 tggtatccac acgggcataa accaacaaag gtttacccgg ctgatagagc gtaaccaatg
     1321 gttcttctgc acagaagcca aaacaacctg ttggttttat gatgcaatca tattttcgtt
     1381 gtgccgcatg cttttgcaag gtttcgtaaa gagcatcagc accatttccg ataccgcaag
     1441 ttcccatacc caccatcacc ataggtacat ccggcaggat atttttcttc agggaactgc
     1501 tgaattgttc gaagttattt agattgattt tttgcataag aggaagattt aacttttttc
     1561 aaaattttca gcaacttagg tgcattcaca ttactgtgga taacgccatc aaccgcaact
     1621 accggcgctt gtgcgcattg tccgaagcaa gcaaccgtgc gaatggtaaa ctcattgtca
     1681 ggggttgtgt agcttggttc cgtttcatca aactcgtaac gaaaacccaa aatttgcccc
     1741 acgctgttga gcaattcgcg agaacccttg gtatgacaag cagtgccgcg acaaaccact
     1801 atcgtgtgct ttccctgagg ttttaaatta aaaaatgaat aaaaagtagc aacgctatat
     1861 acctgagtgg caggtacatt taatttttta gctacttgta acaaggtttt ttgtggaaga
     1921 aattttaaag gatttgcttc ctgaacagct tccaaggttg aaagtaaaac gccggggcta
     1981 cccttgtact ttccgactat ctcgtcaagt actttctctt ccacctcata aggagttagt
     2041 gtgtccatat gcttatcaga tttgaattaa taatttgatt gttgaaaaaa acaaaataag
     2101 acgggcttat ctattaataa tattattact tgcaaaagta acatattgat tacatacaat
     2161 tacacgattt aacattaaat taatgatttt ttttcgttaa atataatatc tctaaataaa
     2221 gagaaaaatc tacaattgca ttataccatt cgagaatttt tttttgacta taagtgatgt
     2281 caagctatta catcatatta catcatactg cgcataggca tttgtagcaa aggctactct
     2341 tctttcttct caacaagaat acattaggta gttaagaaca tactttcaat ggaaaacagc
     2401 tttttctgat gcaaagatga tgtttcgttt tatatttcat aattgggcgc attattttta
     2461 aaaaagattg taacgagtac ttacaggttt ttttgttcaa aaacgtaaat cgaatcaatc
     2521 aataaaaagc tatgatttgc ttnnnnnnnn nntaattatt gattagttta gattaatctc
     2581 aatttatttt ttcttaaatg ctaagtccat gttaaaagtt tatcatgaaa aaggagcttt
     2641 ttttctaaaa aatgtttttt ttaaacaatt ttatgattcc ttagtttttc actgaaagtc
     2701 aatatttaac gagctttcat taagatggtt gtaattaatt ggtgggaaaa aatgtaaata
     2761 tttatcaaca attataaaaa agtgggaaat attgtattac atttggcgat aatttttaaa
     2821 cccactaatt gtgtttactt caacttacga atgtaaaatt gatgataaag gcagactggc
     2881 gcttccgggt aagcttaagg ctgaactgat gccggtttta gctgatggct tcatcttgaa
     2941 gagaagtgtt tttagccctt gtctggaatt gtggccaaaa aaggagtggg ataagacctt
     3001 gacaaggata aatgcgttga cgcggttttc gcgggagaat gcaaaaatgg ttcgtgcttt
     3061 ccttgctggc gtaaagcaag ttgatttaga tggtacagga aggataaata tccccaatga
     3121 cctgatcatt tttgcaggta ttcaaaaaac agttgtaatt gcttcacaga tcagcattat
     3181 cgaaatttgg gacaagtcaa aatacgaaga ggaggtttcg atagctaatg atgttgagtt
     3241 tgcccaaatg gtggaaaata ttcttggcgg tttagaacct gataacacca tttagctatg
     3301 tatcatcagc cggtaatgtt aaaagagagt attcaggggc ttaacattaa acccaacggg
     3361 atttatgttg atgcgaccta tgggggtgga ggacattcgg cagccatact ccaagggatg
     3421 ccaaagggca gattgatcgc cttcgatcaa gataaagatg cgctggtaaa caggataaat
     3481 gatgtacggt taaccttggt aaatcataat ttcagatatt taaaacagtt tctaaaatat
     3541 tataattcac ttcctgctga tggaataata gctgatttgg gcgttagctc gcatcagttg
     3601 gatagcaagg agcgcggttt ttcaaccagg ttcggcggag accttgacct acgtatgaat
     3661 cagaaccaaa aagttaacgc tcaatatatc gtaaatgagt acaatgaaga gcaattagcc
     3721 tttttgttta aaaattatgg agaactatcc gcctatagcg ctatagctca tgaaattgta
     3781 aaagagcgag cctccaataa aatcaagctt ttcgaagatt taaaacgcac tatcgaacat
     3841 ttagccccta aaaaagagga aaacaaattt tttgctcaga ttatgcaagc tttgcgtata
     3901 gaggtaaatc aagagattga ttcgttaaaa gagttgttgc aacaagctgt cgaagtttta
     3961 cgacaggggg ggagattggt agtcatttct tatcattcgt tggaggatag aatagtaaaa
     4021 aacttcatta aaactggaaa ttttgaaggt gtagtagaga aagacttttt tggaaacaaa
     4081 aaattagttt ttaagagtat cacttcaaaa cccattgtac cctctgcgga ggagataaaa
     4141 caaaatagtc gttcgcgtag cgctaaactc aggattgctg aaaaattata acacccttac
     4201 gatgactcga aatgcaaaca tattgaagtc cgaacaaaag caaattgctt caaaaaagaa
     4261 aaagagaacg atatccgagc atgttcattc ttttctcgac gggtcattta ttgcacaagg
     4321 taagattaga ggcagaatat tgtttttact ctttttagtt cttttaggat tggggtatat
     4381 taggaataac tatttagttg agcgtacaat aagaaaattg aataagctta ataatgagtt
     4441 agttgagctt cattacgatt atattcaaat gaaatcaaca gtaaatctca ggacacagcc
     4501 ttcggtgctt gaagaagaac ttcaggctta taatatcaag cgattagaga gaccgccaat
     4561 taagatcttt ttacaaccct gaaattcatc cttatgaaag agaataataa aacaacgaaa
     4621 aaggagcggg ctggtcttga caagggtgta atgtcccgaa tttatatcgt atatgttttt
     4681 ttgctgttaa tagcaatcgg tattttttta cgtatcgtcc aactacagat ttttaaggct
     4741 aatgagttaa atattattgc cgaaaagcgc gagtttcgtt ttgatgacaa tattgaagct
     4801 atgcgtggca gcatttattc tgaggatggg cgcctgatgg caacttctgt tcctgttttt
     4861 gaggtaagga tggatgtggc tttaccggat attaaagata gtgtttttaa tgatagcgtt
     4921 acttggttag cgcttgggct ttcgaaaatg tttggcgact atacaaactg ggaatacaaa
     4981 caacgtctta ttaaagccag aaatgaaaaa aatcggtttt tcctaattca gaaaaatgta
     5041 acttttgatc agcttacaca gcttaaaaca ctcccattgt taaaacgtac tagggattac
     5101 aattccctaa tagtaattcg aacgccgaga agagaatatc cttttggcat acttgccagg
     5161 cgaagccttg gatatttcaa ggatcatcca acggattctt tgaaagtttt ggtaggtgtt
     5221 gaaggagagt ataacgaata tcttcaggga ataaaggggc gtcagcttaa acagaggatg
     5281 gcttatgggg agtggaaacc aatagatcat ttgaataata ttgaacctgt taatggaaaa
     5341 gacattgtgg ttacaataaa cagttattta caggatattg cccacagttc tctgaaaaat
     5401 caacttattg ctcataatgc tgataaaggt tgcttgatat taatggaggt cgaaaccggt
     5461 gaattacgtg ctttggtgaa tctgatgaaa gatacgaaag acggcgagtt taaggagagt
     5521 tacaacatgg ctgtcggcga gcttttcgaa cccgggtcaa tctttaagtt accagcaatg
     5581 atggtagcga tcgaggatgg agttgtgcag aaaattgatt cggtctatat aggtaatggc
     5641 gaggtgaaat acaataacag agtgatgaag gattcgcaca ggtttgatcc tgatggatgg
     5701 gtaactcctg aaaattgcct ggcacattcg agcaatgttg gagtatcgaa aatcatttat
     5761 gatcattaca acggaaacaa taagtctttt tataataaat tgactaaaat gttctcggct
     5821 gatattaccg gagtggatat ccccggcgaa ccgaagccgt ttatcaaaga cccacattta
     5881 aaggacaaac gtaattattg gtcgtcagtt acattgccat ggatgtcaat aggctatgag
     5941 gttcttacta caccacttca aatgctgacc ttttataatg cagtggcaaa caatggaaag
     6001 atggtaaagc cgcgaatagt aaaggaagta agagaaaatg ggaacactgt aaaagtattt
     6061 gaatctgaaa taattaatcc ttcaatatgt tcgcaaagta ctgttgcatt ggcaaaacac
     6121 tttttactat caacagtaga aaatggtacc ggtaggaatg ttaaaaacaa tgattacaaa
     6181 attgccggaa aaactggtac agccaagatc aatgaaaatg gtagatatat ttccaaatat
     6241 aatgcatcat ttattggata tttccctgcc gacaaaccga aattttcatg tattgtagtt
     6301 atttaccgac caaatgaagg cgcttattac gcatcgcagg ttgctgctcc ggtgtttaaa
     6361 gaggtggcag acatggtttt tgcatttgcc tataatattg atcctctaaa taaaaaccag
     6421 tttatcgctc ataaagacga aaatggcgct cctatgaata ggctgcctga tcaggttaat
     6481 cctcaactcg ctgagctggt gaattatgaa ggtgtaaaac ctttggaaga gagtaagtgt
     6541 cctatggtta tcccagatgt tactggcctt agtgttaagg atgctacaca tttgttggag
     6601 gcttgtaatc ttgtagtgaa gatacatgga aaagggttgg tgaaaataca gtcgccagaa
     6661 ccgggaacgc catataaagc aggtgatatg gttgaactta aacttgaaat catatagaag
     6721 catgatggta cggcaattaa ataaaatatt ggaaagtatt gatttcttac aaagagttgg
     6781 aacaacagat tcgatactga gaaatataag tttcgactca cgtactgtag gccatgatga
     6841 tttatttgtt gccgtcaaag gtgtgaagat ggatgggcac gattttatcc ccgaagttat
     6901 taataaaggt gttgccgccg taatttgtga acggatacct gataatatgc cggaaaatat
     6961 cgtctttatt aaggttagga atagcgctgt tgccttaggg caggcggcat cagcttggta
     7021 tgatcaccct tcgacaaagt tgaaagtgat tggaattaca ggcactaatg gaaaaacgag
     7081 cacggtattt ctgttatatc atctctttaa gggtttaggt tttaaaacag gcctgctttc
     7141 gacaattgag aataggatag gagatgaaaa agtagcttca acacatacaa cagccgattc
     7201 aatacaaata aataagaatc tggctaaaat gattgaatcg ggatgccatt attgttttat
     7261 ggagttaagt tctcatgcca tagatcagga gcgaatagcc ggtttgaaga tcagcggcgc
     7321 tattttctcc aatatctctc acgatcatct tgattatcat gtaacattag acaattatat
     7381 aaaagccaag aaaaagctat ttgatgaact gccgaccgat gcttttgtgc ttgttaatgc
     7441 agacgatagc catagtaaaa taatgttgca aaactgtgtg gctcgtaaat ttaattatag
     7501 ccttaaaagc ccgtcagatt ataagggaag gatactcgaa aacagttttg atggattatt
     7561 gttgcgaatt gataatcaag aggtatggtg taggttagta ggaggtttta atgcttataa
     7621 ccttttagca acttttgctg tggctcgtat tgagggattc caaaatcatg aagtattacc
     7681 attgttgagt ttgcttgcgc ccgttgaagg tcggttccag attattaggt caaaggccgg
     7741 aattactgta atagtagatt acgctcatac gccggatgct ttaaaaaatg tactgaagac
     7801 aataaacgaa gtaaggagtg gaaaagaaaa actgataaca attattggcg ccggtggcga
     7861 ccgcgacaga ctaaaacggc cgctattagc taaaattgct tgtcggttaa gcgaccgagt
     7921 tatactcaca tccgacaatc ctcgaactga aaaacctgaa atgatcattg acgatatgga
     7981 agcaggattg gatagggata tgaagaaaaa aacaattaga ataactgatc gcctcgaagc
     8041 cattaaaaca gccataatga tatcgcttaa agacgatata atactgattg caggaaaagg
     8101 ccatgagaca tatcaggagg ttaatggtgt aaagagtcat tttgatgacc gtgaaattgc
     8161 agtagaatat ttgaataact taaattccga taactgatgc tctattattt ttttggatat
     8221 cttgataaag tatttgattt tcccggagca cggatgtttc aatacatctc atttcgagct
     8281 gggatggctg tcattgtttc actgatcatc tccatgattt taggaaaaag ccttatcaat
     8341 ttgttgaaaa aaatgcaggt gaaagagagt atcagggatt taggacttga aggccagaaa
     8401 gaaaaagagg gaactccaac aatggggggg cttattattc tcggagcaat actaatcccg
     8461 accctattat ttgcagacct taccaacata tatattatca tcattttgat tacaaccatt
     8521 tggttaggct ttatcggctt tattgatgat tacattatgg tttttaaaaa ggataaaaaa
     8581 ggtttagctg caaagtttaa aatccttggc caggttacgt tagggctttt tgttggtttg
     8641 atgatgtatt atcatccaga tattgtaatt agagaaagaa cttctgatat ctcttttatt
     8701 gaatcaatag aaaaggttga aacagatgat ttgagggatg ttgctaatga tctttactcg
     8761 aagaaaaata ttaaatccac taaaagcacg atcccatttt ttaaaaacaa cgaactcgat
     8821 tattcaattt ttgtctcctg gttaggaaaa gactatcagc gctggacaat tattgttttc
     8881 gtggctgttg tgatattgat cattacattg atatctaata gcgctaacct tactgatgga
     8941 atagatggct tagctaccgg tgtgtctgct ataatcggct caacacttgc tgttttggct
     9001 tatgtttcgg gtaatatatt ctttgctaac tatctgaata ttatgtattt acctgatagc
     9061 ggagagctcg ttattttcat tgcagcattt gttggagctt gtattggttt tttatggtac
     9121 aacacttttc cggctcaggt ttttatgggc gataccggtt cgttagcctt aggaggaata
     9181 atagcagtat ttgcaattat gctgcgcaaa gagcttttaa taccactgct ttgcggcatt
     9241 ttcttagctg aaagcgcttc tgtagtcatg caggtaagtt atttcaaata taccaaaaga
     9301 aaatatggcg ctgggaagag aatttttaaa atgtcgcctt tgcatcatca ttatcaatta
     9361 aaaggctatg cagagccgaa aatcgtacag cgatttttta ttgttggaat aatgttggcg
     9421 gtatttacag taataacttt aaaaataagg taatgtaagc tatgtcgaat tcaattgcaa
     9481 tacttggagc tggagagagt ggaacaggcg cagcagtttt ggccaccaaa aaagggcata
     9541 gagtatttgt ttcggataaa ggtttaattg aaaataaata taaagaagtt ctttcacaat
     9601 acagaatcga atttgaagag ggtaagcata ccgaggagtt gatatttgca gcagatgaaa
     9661 taataaaaag tccaggaatt ccggatgaaa tagatctgat cagaagggtc aaagcgctga
     9721 gtaagcctgt gatctctgaa cttgaatttg ccggaagata cacaagagca aaaaagttgt
     9781 gcgtaacagg gagcaatgga aaaacaacta ccaccttact gttataccat attttgaaaa
     9841 atgctggttt ggatgtaggt ttatgcggca atgtggggaa aagtatggca atgcttttgg
     9901 ccgaacgcga ttatgattat ttagtgattg aggtaagtag ctttcagttg gatggcatgt
     9961 ttgattttaa agccgacatg gctattctta ccaacatcac tccggatcat cttgacaggt
    10021 acgacgggaa gtttgaaaat tacgcagcat cgaaattcag gattattaaa aatcaaagag
    10081 ataccgacta tttcatttat tgtaacgatg atccggaaat tttaaaccga ttagatattt
    10141 ataagccctc ggcaagttgt tacccttttt ctatcgccgg agatataaaa agagagggcg
    10201 cttttttaga tagtaaacat cagcctaata aaatgtattt caatataaaa tctgacatca
    10261 tgataatgac tttagaggaa ttggctctaa aaggcatgca caatgcttat aatactatgg
    10321 ccgcaggcat agcagcacgt cttcttgata tccgtaaaga gtctatcaga aatagcctcg
    10381 ctgattttca aggtattgaa catcggcttg agtttgtggc taccatcaga ggtgttgatt
    10441 ttgtcaatga ttcgaaagct actaatgtta attcggcatg gtgggcgctg gataacgggt
    10501 ataagccaat aatttggatt gccggtggta ttgataaagg aaacgattac tctgagatag
    10561 ccgaccttgt ggctaaaaaa gtaaaagcaa ttatttgtat cggtgtaaat aatgacaaga
    10621 ttatcaggca actgggcagg tatgtagatg tgtgtgtgga ggctgcttca atgcaagaag
    10681 ccgttgaaat ggcctatcaa cttggaacta agggcgatca ggtgttacta tctccggcct
    10741 gtgccagttt cgaccgattt aataactatg aagacagagg aaatcaattc aaaaaagctg
    10801 ttataaatct ttagcctttg atgcgatgaa taaaacaata attaaaggag ataaagtaat
    10861 ctggatgata actctctttt ttgctattac ttcactattg gtagtatata gctcaacaga
    10921 gacattagcg tttcaaaaca agacaacaac cgaaagactc cttttaaaac aggctattgt
    10981 aatgctttgt ggtattggct tactttattt cgcacacacc ctcaaatatg tttactattc
    11041 ccgcattagt caatttttga tgatattagc gggggctttc cttttaatga ctttttttgt
    11101 cgctcccgag ataaataatg cacaacgctc tctcagattt actttaccaa ttgtaggaca
    11161 aatatcgttt caaccttctg atctggcaaa gcttgcgatg atgattttca tagcgcgttt
    11221 cctatcgaaa aacaacacac aaaagatggg atttaaggag atgtttctca aaattgggct
    11281 gcctcttttc attattacgc tattaattat aaggtctaat ttctcaacgg catttcttct
    11341 ttttggggta agtattgttt ttctttttat tggaaaagta aaaataaagc atttaagtta
    11401 cttgattttg attgctgttg ccggccttgg gatgataatt cttatcggat taataaaacc
    11461 ggaggtgttt ccaagggcta aaacttgggt taatcgagtt tcggcatttt caatgtcgag
    11521 tgaaaaagaa gaacctaacg ttcagattgt acattctgaa attgcaattg ctcagggtgg
    11581 tctttttgga aagatgcccg gcaaaagtac tcaacgtatc tttttacccc ttgcctttag
    11641 tgattacgtt tatgcaatta ttatcgaaga gtacggctta gtgggaggtt ttacagtgct
    11701 tttattgttc acggcattgc tctttaggtc aataaagatt gcaaggcgat gccagatgaa
    11761 gtttggaggg ttcctggttg ttggaataag cctgatgttg gtttgtcagg cattgtttaa
    11821 tatgcttgtt gcagttaatt tggctcctgt aacagggcag cctctgccat ttctttcaat
    11881 ggggggaaca tcgttttggt tttcatgtat cagcataggg ataattctta gtgttagccg
    11941 ctctttagaa ttggattata aacaacaata ttcagaagat cttttagcca atggtagcga
    12001 atgaattgaa aatattaatt tctggaggtg gaacgggagg tcatatattc cctgctatag
    12061 ccattgccga tgcgcttagg aatatagttc ccgactgcga tattttgttt gtaggcgcat
    12121 taggaaaaat ggagatggaa aaagtgccgc aggctggtta ccccataaaa ggattacgga
    12181 tcagcgcttt tcagcgtaga ttgtcactaa aaaatttgtc ttttccattt aaagttgttt
    12241 ttagtatggt taaggcatac aaaatcatca cctctttcaa gccagatgtt gttgttggtg
    12301 ttggcggttt tgcaagcggg ccgacattaa aggtggctgt taatcgtcgt attcctgtag
    12361 tgattcagga acagaactcc tatccaggca ttacaaatag actgttggca aaacatgctg
    12421 aaaggatttg tgtagcctat gacgggatgg agaagtggtt tccggctgga aagattctta
    12481 aaaccgggaa tcctgtcagg gaaaatgtaa ttaatattca aaataaaaag gatgaagctt
    12541 ttagatattt tgaattagaa agcgggaagc cggttgtttt gatcgtggga gggagccagg
    12601 gagcgctatc aattaatcaa gctcttagtt acaatcttaa agatttggaa cagtgtgggg
    12661 tgcagatgat ttggcaaaca ggcaaattgt ttgctgataa ggcaaagtct gcaatacagg
    12721 aaaaaatcgc taatccgcgg gataaagatt ttcatgtgta tgaatttatt acacgtatgg
    12781 atctggctta tgctgctgcc gatctggtaa tttctcgcgc tggagcgatc gctatctcgg
    12841 aacttagcgc agttcaaaaa ccttcaatac tcattccata cccagcggct gccgaagacc
    12901 atcaggcaaa aaatgcaaaa gcgcttgctg ataaacaggc tgcaatcttt ttggctgata
    12961 gcgaggcaaa aattaagata gcaacaatag ttaaaagctt gatagaagat gaagaaaaaa
    13021 gagatacact ttcaaaaaat attggagagt ttgccgtaca caattcggcg caaaaaattg
    13081 cagaacagat aattaaaata gcaagcaaaa gaaatggagt ttaaggaata ttcacatata
    13141 tttatgttag gcattggcgg cattgggatg agtgcactgg ctcgttattt caaactaaaa
    13201 ggaaaatttg ttgccggctt tgatcgtgtg aatactcagc ttacacaaac ccttgaaaat
    13261 gagggtttca ctattcttta taaagataac gttgatgaaa ttccttatga cttttccata
    13321 cctgaaaaaa cacttgtgat ctatactcct gcaataaaaa atgataatac attgttgacc
    13381 tatttttccc aaaggcagtt taaggttttt aaacgcgccg aaattcttgg cagaattgct
    13441 ttggaatata aaacaattgc tgtggctggc acgcatggca agacgaccat cactgcaatg
    13501 gtcagtcatt tgcttcatca atcggctgtt gaagtaaatg ccatggtggg tggcattatg
    13561 aaaaactacc aatcgaattt tattgcatcc gcaaaatcag agtttttagt taccgaagct
    13621 gatgaatacg accgctcatt tctaaaactt tatccccatt ttgctgtaat aactgcttgt
    13681 gatgctgatc atcttgatat ttatggaacg gaagaggagc ttttaaaggc ctatggaaaa
    13741 tttgctgatc aagtgtcagc cgaaggatta ctattgacga aaaagggact tcaactcaat
    13801 gtgcaaaatg cctcggttac acgtaaatat acttattcgg tcaacgatcg gtctgctgat
    13861 atctatcctg atcatttgca atttaatgag aatatgatct cttttgatct tgttactccg
    13921 caaggagttc ttgaaaagct aactatgcag cctacaggct taatgaatgt agaaaatgct
    13981 gtagcagcaa cttttatagc gcttcaagca ggagccgaca gggatgagat aaaagaagga
    14041 ctttcaactt ttaaaggagt tcgaagacgt tttgatatgc tttacaatga caacgaaaca
    14101 atttatattg atgactatgc ccatcatcca caggaaatca ggtcgcttgt caaatcagta
    14161 aagtcaattt atccttcacg acatattaca ggaatttttc agccacatct ttttagccgg
    14221 acattaaatt ttgcggatga atttgcaaga agtttagagc aattagacac aattatactt
    14281 cttgatattt accctgcacg agaaaaacct atagaaggag taaactcagc tttcttgttt
    14341 aaaaagataa atcataataa taaattctta tttgccagag atgaaacgat agaatatctt
    14401 ttacatcacc attttgaagt attgctaaca attggcgccg gtgatattga cactttggct
    14461 caaccgcttt tgaaaatgat caggcataaa aaagtaataa aagagaaatg atgaaaaaga
    14521 ttcttcaaat atcattttgg ttgattattc tggcagggat cattgtcatt ttcggttttg
    14581 ctgtttcaga acagaaaaaa actgtctgct ctggtcttgt tataaatttg aaagatgctg
    14641 aacagcctgg atttatttat gagaaagatg ttagagctat cattagtcag ctatatcctt
    14701 cgcttagagg aaaaatcctc gacagtttaa atacggctgc gattacaaaa gaactaaatc
    14761 gttctccata tcttaaaaac ccgattgttg taaaaacctt aaatggcctg ataaaagtac
    14821 agcttgaacg aacggaggct ttagtgcgag ttgtaaacag caagggcgat tctttttatc
    14881 tgagtaaaga gggttacgta atgcctataa gcaggagtta tatacctaaa acactgattg
    14941 ttaccggaca tatttatgat agccccaatc cttatgataa attaatttac aatgttaatg
    15001 gtggtacagc gtcaaaaaaa tccattctga ataagatatt ttttcttgca gaaacaatca
    15061 ttaaccacag tgtttttaac caaagtattg atcagattta tgttacagat tgtaatgaat
    15121 ttgaaatggt tccctcttcc ggtgatcata ttatcctatt aggcgatgtg gaaaatttaa
    15181 accataagct tgagaacctg aatgcttttt atcaggccgg gatatcaaaa ttggaagaag
    15241 gggagtataa aatatttgat ttaagattta aagatcaggt agtttgtaaa aaattaatgc
    15301 catgaataca gcgaatattg ttgttggcct cgatatcggg accacaaaaa ttgccgccat
    15361 tgttggcagg cgttcagaat atagaaaaat tgaaatcctc ggttatggcc gcacagagtc
    15421 tattggtgta aaacgcggta ttgtgtccaa tatcgaatta actgttgact caataaaaag
    15481 ggctattcag gaggcagaag caaaatcgga ggtagatata aaatatgtga atgttgggat
    15541 agccggacag ctaataaaaa gccttttgca taggggcgga ttggtaagaa aaaatattga
    15601 agtggaaatt agtcaggagg agattgataa tttgctcaat agtatgttca acctgaatat
    15661 gaatccggga gaggaaatta ttgatgtaat accgcaggag tatattattg ataatgagcc
    15721 ggatattcgt gagccaatcg gaatgcttgg gagctcgttg gaggcaaatt ttcatattat
    15781 tatcggacaa acgacggcag caaagaatat ttacaaatgt ataactaaag ccggtttgga
    15841 agtagttgat cttatccttg aaccaattgc ctcagcttgc gcagtgttga gcgaagagga
    15901 aaaagaggcc ggcgtagctt tagtggatat tgggggaggt actactgata ttgctatttt
    15961 tcaggatggc ataataaggc acacagcagt aatcccgttt ggcggcgatg tattgactga
    16021 agatgtaaaa gagggatgtt ccattatacg aaaacatgct gaagagttaa aaataaaatt
    16081 tggttcagcg ttggctaacg aaaacaaaga tgaagagatt gttgcaatac ccggtttgcg
    16141 cggaaggcca cctaaagaga taagcctgaa aaaccttgca agtattatac aggctcggat
    16201 ggaggagatt attgaacata tctattacga aataaaaaac tctggctacg aaaaaaagct
    16261 gatagcgggg atagtgctta ccggtggtgg ctcccaattg cgacatctgg cgcagttgac
    16321 cgaatttatg actggtatgg atacaaggat cggttatccg aatgaacatc ttgccggtaa
    16381 tgctcccgat gaaatgcaca gcccaatgca tgctacgggt ataggattgg tgcttgaagg
    16441 cgtagcaaga ttggaaaaac aacttgaaag agatagtgaa atcaatgacg ataatggacg
    16501 gagtggaaaa gcgaaaatat cagaaaaaag cgaaaagaag ggcaaaaggc caatcggctt
    16561 tttacgaaaa atccaggagt ttttcgaaga cggtcaggaa taaaagataa acaacaaaat
    16621 aaactacaaa gcaatgaacg acatgttatt atttgatgcg ccaaagcaaa gggcgtctat
    16681 cattaaggtt attggagtgg gcggcggtgg cagtaacgcc gttacgcaca tgtataaaca
    16741 aggtatcaaa ggtgtagatt ttgtaatttg caataccgat tctcaggcga tggacataag
    16801 cgccgttccc aacaaaatcc aacttgggaa aacaggcctt ggagcggggt caatacctga
    16861 cgtaggacgg ctctccgcag aagaaaatat tgaagagata aaaaagatta tagagagtaa
    16921 cacaaagatg ctttttgtta cagccggcat gggtggagga acaggaacag gcgcagctcc
    16981 agtaatcgca aaacttgcca aagagatggg aatacttacc gttggaatag ttaccctccc
    17041 ttttaatttt gaaggcagaa aaagacgatt acaagcagaa aaaggaatag aagagcttaa
    17101 aaagtacgtg gacactctct tgctcattag taatgaaaaa ctaagagaga tacatgggaa
    17161 ccttaaaata tcggaggctt ttggtaaagc agatgatatc ctcactactg cagcaaaagg
    17221 gatcgccgag cttattacag ttgctggata cattaacgtt gattttgaag atgtgaaaac
    17281 agtaatgcaa aacagtggaa ccgccattat gggctctgca tctgccgaag gaaatgatag
    17341 agctagaata gcagtaaaag aagcgctgac atctccatta ctcaacgaca acaaaatagt
    17401 aggagcaagt aatattttac tatatatatc gtcggggaaa gaagaaatta ccttcgatga
    17461 ggtaaccgaa ataaccgatt atatacagga ggaggccgga gaaaatgctg aaataatatg
    17521 gggaaatggc tatgacgatt cacttggcga taaaattagt attactctta ttgccacagg
    17581 atttttgtcg ccagaagaga ttgatgaaaa tacacttaac cgtcaaaaga aaaaaagagt
    17641 gtataatctt aacgatgatt tgccgaacaa acagagtaac gaacaggctg aactggaaac
    17701 attaactatt gttgagactg ataataagca agacattgaa tgtaagccaa ttgaaattac
    17761 agaaataaca ttgttaaaat ccgaatcggt agataataaa aaagtagcta accccacaag
    17821 agagatcaat ttgggtttat ttgaaaacat tgaaaacaaa atacaaagta aatcaagtga
    17881 tcaatcatca tcgcaacaag ttccggtaag gccttttaat gatgggcctc ttaaaaattc
    17941 ttccttaaaa attaaacata ctgttggcga tccaattcct tttaatgatc tttcatcgaa
    18001 acgagaagag ttggcgcagc aaaagagtgt gatagataaa aaagctgatg atagggtcga
    18061 gaaactgaga agtttaagta tttttggaga taagtttgcc gataaaattg atgagatgga
    18121 gaaggtaccg gcctatatgc gccgaaacgt acaactaaat ccggtaacac cctcttccga
    18181 atcggcaata actagatata ctttgagcga aaacgaagat tttgatgctg agataaaatc
    18241 taatgaaatc ccctttctac ataataagcc agattgatag ctttgattaa ttattttgat
    18301 atttgtaaca taaaaatttg gaaatcatga gtttagttga aataattaat gaggatataa
    18361 aaaatgcaat gctctctaag gataaacgca aattagaggc gctgcgcgca ataaaagctg
    18421 ctttgctact cattaaaacc ggaaaggata aaagttcaga cgaaatacct gagacactcg
    18481 aaatgcagat gttgcagaaa cttgtgaagc aacgccgtga aaccgcagtt atctatcagc
    18541 agcaaaatcg tcaggaactt gctgacgaag agtt
//
LOCUS       DAOOAI010000038        18349 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_4027_length_18349_cov_5.379414, whole genome shotgun sequence.
ACCESSION   DAOOAI010000038 DAOOAI010000000
VERSION     DAOOAI010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18349)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18349)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18349
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4027_length_18349_cov_5.379414"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..298
                     /locus_tag="PK345_04155"
     CDS             <1..298
                     /locus_tag="PK345_04155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HPX34249.1"
                     /translation="FNGTHNEYSSGYLTELMAEAKIAKDDPRVWFSQLYGMSDHISFN
                     LAHNGYNVVKYLPYGPVKYVIPYLVRRAEENTSVKGQTGRELSLIKKEIKRRKR"
     gene            complement(314..1234)
                     /locus_tag="PK345_04160"
     CDS             complement(314..1234)
                     /locus_tag="PK345_04160"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPX34250.1"
                     /translation="MNLTKSLKAHLSLLLAAMIFGANYWISKGLMPDYLSPEQLLFLR
                     VVGSFLLFFMISFMLPSEKVDKADKKTIALASLLGVTLNQLLFFIGLNLSTPVDVAII
                     HVSNPIFVLIIASFLLRENVTFLKIIGIGMGAAGAVLLITYQGELSFSSNTFTGNLMA
                     LLNTLAYSFYLIIIKPIMKKYSPITVMKWAFLYGTIFSLPLTLHSAIDISFEGFTINT
                     WFSLFFVIVLTTFMAYLLTIYGLKYLDAGVVSYYVYLQPIIVTGIAYFISSNPLSWQH
                     IIAASLIFGGVWLVSLKNPRLTTPLIFKNR"
     gene            1466..3196
                     /locus_tag="PK345_04165"
     CDS             1466..3196
                     /locus_tag="PK345_04165"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPX34251.1"
                     /translation="MRKIILTVIATIFFGVGFHFTANACTNYLITKGASADGSTMISY
                     SADSHVLYGELYHWPAGIWPEGTMMDIVEWDSGKYLGKIKQARQTYNVVGNINEYQLA
                     IGETTFTGLEMLESQEGSLIDYGSLIYITLQRAKTAREAIKVFHELTSTYGYHSTGES
                     FSIADPNEVWILEMIGKGNGYKGAVWVARMVPDGYVSAHANQARITTFPLADGKISVT
                     EKDFDKFMKNSAITTIYSNDVITFAREKGLFKGEDKNFSFSDTYNPVDFGGARFCEMR
                     VWTMFNRVTTGMDQYFDYVKGKIRHDDKLANGDVNENRYATNRMPLWIKPDKKVKLED
                     MFEFMRDHLQGTELDMSKDFGAEPFGLPIRWRPLTWEVDGVTYCHERVTVTQQTGFSF
                     IAQMRSWLPNPIGGILWFGVDDASCSIYMPMYCSITKAPYSIERGHGAMMTWSDDAAF
                     WVFNQVSNFAYTRWNIIYPEILEKIHNYEQEFIINTSTIDTKALALFKENPESAINYL
                     TDYSTRLGDKVTDDWRHFYRYLFMKYMDGNKKTPIDGEMNPKVEWPGYPEKWRRKVVE
                     ETGDQFKVIE"
     gene            3285..4190
                     /locus_tag="PK345_04170"
     CDS             3285..4190
                     /locus_tag="PK345_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34252.1"
                     /translation="MLEKEKDRQKLIYRYAAIVFLVLLIIIGILYIIKTAKIRELNAE
                     KEQIRIDLQSELDSLMLQHNHIKKEYGILTDSLVVKDSLIQANAKEITELLNYKWEYY
                     QIKKKLDRLRVVAQGYVYQLDSLYTVNKELQEENERIRENYQTERQKNIGLTKEKHEL
                     INKVTEAAVMKAYNIKSTGLRYRGARQEETDRARRTDAVRICFTIGENSLITPGKKDI
                     YLRIARPDNFILIYDDTDYYTFFHEGNKLQYSLKHTIDFTGKAQNVCVVWNKHNQDEK
                     TMAGIYHVMVYDEENLIGETSFELK"
     gene            complement(4217..4819)
                     /locus_tag="PK345_04175"
     CDS             complement(4217..4819)
                     /locus_tag="PK345_04175"
                     /inference="COORDINATES: protein motif:HMM:NF021254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archaeosortase/exosortase family protein"
                     /protein_id="HPX34253.1"
                     /translation="MKNLSEILSKAKSFIKRYHLYIFKDVALFILITVVVHFSYRFWV
                     KKMHYWPIKTTINNLESNMSKVVYYHSAWFVENVLNIEITCIDQTKTMYFVNNGYISV
                     NRGCSGFKPILQFIILMMFFTGSLKNKLWFIPLGVLAVHLTNLFRIIGLSVVITKWPE
                     YWDFSHDYLFRPFFYVVIFLMWVWWVEKLAYPKKEKKAAN"
     gene            complement(4870..5388)
                     /locus_tag="PK345_04180"
     CDS             complement(4870..5388)
                     /locus_tag="PK345_04180"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HPX34254.1"
                     /translation="MDFFELILNRESVRSYDPHRPVESDKIQKILKAGHLAPSAANRQ
                     PWRFYLVSTPEYLDKVRDCYHKSWFKKAPHLLLVTGKKDLAWTRSSDGFNSLEMDIAI
                     AMDHMILQAESLGVSTCWIVAFDTEKLHQLGIVEEGEVVYAITPLGYPEKDFKKHGNK
                     VRKSFNEVVKFL"
     gene            5664..7085
                     /locus_tag="PK345_04185"
     CDS             5664..7085
                     /locus_tag="PK345_04185"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HPX34255.1"
                     /translation="MKYLFGLFFFVLTAIIPPSCTFNHDIAGEDMFNVKDQSLNRILE
                     RGTLRASTVYNSIDYFIYRSEPAGYQYELLSSYARYLGVNLEIVTNQDVNSAFQDLWS
                     GRVDVIAQGLSVTGNRKKHLDFTYPLAQTHQVLIQRKEGYSNKFSIDGVAADFINSPL
                     DLAGKIIFVPQGSSAASRLRNLQDEIGGKIYVVEYPYDTESLISMVATGKIDYTVCDD
                     KTASVNQKFYPHLDTSVPVSFSQNIAWAVNIGCESLKKSLDEWLEGYLNSANGRFVYN
                     KYFIYPRSHLSEYSFTPSKNGKFSPWDDFLRSKSANYDFDWRLVASLIYEESQFQPDA
                     ISVKGAFGLMQFMPETAEKYKINFESSPEEQIEAGLKYLSELDRQFKKMIKNKEERIK
                     FVLAAYNSGIAHVLDARRLAKKYDRNPNLWDDNVDYFMKNKSNPEYFTDSVVQHGYSN
                     GVVSFNFVNDILNRYEHYKNVIE"
     gene            complement(7261..8865)
                     /locus_tag="PK345_04190"
     CDS             complement(7261..8865)
                     /locus_tag="PK345_04190"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HPX34256.1"
                     /translation="MAEFVYQNPFPVKEDTTKYRLITKDFVSTEEFNGRKILKVKPEG
                     LELLAREAFFDVSFFLRSSHLRQLAAILEDPEASDNERFVAYTMIKNQVVSAEGKLPT
                     CQDTGTAIIMAKKGEDVYTGVDDAMHLSKGIFDVWQQNYLRYSILAPLTMTEEKNTTT
                     NLPAQIDIYSSSGNSYEFLIVTKGGGSANKSLLFQQTKSLLNEENLTKFFKEKISELG
                     SAACPPYHLAIVIGGSSAEANLAVVKKASTGYYDNLPTQGNIYGQAFRDLEWEERIKK
                     ITQESGIGAQFGGKYFAHDVRVIRLPRHAASCPVGIGVSCSADRNIKAKITEEGIFIE
                     ELEKNPERYMPAAPPELSPAVEINLDRPMNEVLEELSKYPVKTRLSLSGTLIVARDVA
                     HARINKILEEGKPMPEYFKNHPIYYAGPAKTPPGLPSGSFGPTTAGRMDMYVEKFQSM
                     GGSMIMLAKGNRSKQVTDACAKYGGFYLGSIGGPAAIIAKEYIKSIELVDFEELGMEA
                     VRKIRVVNFPAFIIVDDKGNDFYKQL"
     gene            complement(8905..10338)
                     /locus_tag="PK345_04195"
     CDS             complement(8905..10338)
                     /locus_tag="PK345_04195"
                     /inference="COORDINATES: protein motif:HMM:NF012470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HPX34257.1"
                     /translation="MITSNRILKIVIFPVIFFFFYSGFLHAQQAKYIFYFISDGMGMT
                     HVAVTEAFLASQKNEIGFERLSFTKFPTTGFANNNALNRLITCSAAAGTALSTGQKTT
                     INTIGMNGTRDQNLYSIASIVKREGYKVGIISNVSINHATPSVFYAHQPSRNNYYEIG
                     LDLISSNYDFFGGGGFYQATGKDKDKPDLYQLAKQAGYHIVRGVDQLNNLKRNDNRVI
                     AISNFLTKAASQDFVIDQNRNDIPLSLLIDKAIELLDNDKGFFIMAEEGNHDWAAHAN
                     DGATAIWNIIGLSDAVERALEFYKEHPNETLIIVTADHETGGITLGWSGKNYETDLLL
                     LIHQKVSFSKFAHLLDSTLKIPENQNYELILKLINEYFGLGGEGELALSDYENKLLSD
                     AWKCWKGELQICDMEKQLNYGNENPIAVTATRILNHKAGLGWTTWAHTGTPVPVFAIG
                     QGHEMFNGYYHITDIPKKIKMAMGLDK"
     gene            complement(10580..11545)
                     /locus_tag="PK345_04200"
     CDS             complement(10580..11545)
                     /locus_tag="PK345_04200"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HPX34258.1"
                     /translation="MRIYKDIADIGKIKNAVVTVGTFDGVHIGHQLVIAKLVEAAKRI
                     EGESVLITFEPHPRIVVQPGYDLRLILSMDERVQIFSQLGVDHLFIIHFTKEFAQFSS
                     EYFLEKYIYEVFKPVKLIIGYDHQFGKERRGDIHFLGQMAAKLGFAVEQIEAEDLAGF
                     AISSTRIRDAIKKGDMQLVSNLLGYYYSISGLVVKGNRLGRTLGFPTANIHLDDPEKL
                     IPYYGVYATLVDYKGTIYKGMSNIGIRPTLNQHNLNIEVNIFDFNKEIYGENLRLYLV
                     EHTRDEKRFRDLDLLRRRLVIDQVKIKKILESVNVDQKLLFTTKK"
     gene            complement(11687..11759)
                     /locus_tag="PK345_04205"
     tRNA            complement(11687..11759)
                     /locus_tag="PK345_04205"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11724..11726),aa:Lys,seq:ctt)
     gene            12059..12778
                     /locus_tag="PK345_04210"
     CDS             12059..12778
                     /locus_tag="PK345_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014606.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015774 - polysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HPX34259.1"
                     /translation="MKKIKYLQSEVKKGDAVQTTFEHKKSEDFKIQPGDNLYIRVNNA
                     LSTTENIFSLDQESRSSNYYNDVGIYLNSYRVSDSGYIDFPFVGKVFLKDHTLEQAQE
                     LLQSIVDDYLKGTTVIVRLAIFKVTMLGEFNRPGEYSVYESKFNIFDAIAQAGDLTTF
                     AKRNKIYLVRQTESGSKVVHLDLNDIGILESEYYTIMPNDIIYAQPVKGKNFAFREFP
                     YSLIFTTISTTLLLINFFKTN"
     gene            12790..15237
                     /locus_tag="PK345_04215"
     CDS             12790..15237
                     /locus_tag="PK345_04215"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HPX34260.1"
                     /translation="METTPNIHNLKQEESVDLKMLFIKFFRYWYFFALTIFVALVIAF
                     LFNKYTKPVYEVKTTVLVKDDRSSKDLIGIGIYDMQNLQNEIGILSSYSLAERTINNL
                     NFEITYQLEENFITTELYDKTPFIVEMDTNTPQLQNVKFTVLILSNTQYRLELQVEEG
                     ILYHFSEREEKGNITGINFTRDFNFGDQVEGEFGKFRIYLTEEFSGDYLNKNLIFIFN
                     DYQSLISQFQGIKVEPIKRDASIVEISIRGSNKCKMKDYLNELTYQYLYRGIEKKNQI
                     ADNTIRFIDSELVDISDSLTFAETRLQDFRASNEVMDLDFKANQVFTIMRQLEDQKAQ
                     LIVKSKYYKNLQDYLIKHKESIDDIVVPSSMGIDDPLLTQLITELAKLYTERAQLLLN
                     SSERNPYVVAIDQRIATTKNTISENIQNIINTSSISIKDIDERIGRLELEIGRLPQTQ
                     RQLFGIERTFKLNDAIYTFLLQKRSEAQIAKASTLPDNEIIDVARGGDQVFPKKGLNY
                     LIALILGIVIPIIYIIGKDYMNDKIIERQDVEKITNIPIIGHLIHNNKETQLVVPESP
                     KSSISESFRSVRTNIQYLTKGKSKQTVLVTSDMVGAGKTFAAMNLAAIFAMYEKKTLL
                     LGFDLRKPKIYQDFGLTSTEGISSYLINKSPLEDIIQVSPVKNLDIIMAGPVPPNPAE
                     LISSDKTDELFERIQEMYDYIIIDTPPVGLVTDAFLLMKYTDANLFLVRQNYTNKKVF
                     ESIIKDIELRKLPNMYILINDVKLGKGSYGYGYGYGYGYGYGYGYGYGYGYGYGYYSD
                     DDETSKKSLLGKIFGKA"
     gene            15586..16566
                     /locus_tag="PK345_04220"
     CDS             15586..16566
                     /locus_tag="PK345_04220"
                     /inference="COORDINATES: protein motif:HMM:NF013408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HPX34261.1"
                     /translation="MNKLYPLKFKPLFKEKIWGGTRIKDMLGIDFGNLSNCGEAWILS
                     GVKYNETVVTNGFLKGNGINELVEVYMDDLVGEKIFEKHKNEFPILVKLIDSNDYLSI
                     QVHPDDKLAAKRGLGNGKTEMWYILSADPEAELISGFKEKIDQQTYLHHLESNTLKQF
                     LNTEKVMVGNVFFIPAGRIHALGPGILLAEIQQTSDVTYRLYDWDRVDGEGKKRELHT
                     KEAIEAIDFNVYKNYKTQYYEVLNKSVELVSQPFFTTNIIHINQPIKRDYTMLDSFVI
                     CTCVQGSSEMIYPDGKVNINIGECVLIPNEIKDILIKPFSDCKILETFII"
     gene            16627..17148
                     /locus_tag="PK345_04225"
     CDS             16627..17148
                     /locus_tag="PK345_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HPX34262.1"
                     /translation="MKKIILLVLVVVPFTTLWAQGGSKALFKLPEANLQTMDGKPFNT
                     KNLQNDGKPILLTFWATWCKPCMKEHDAINDLYDEWIEETGVKIYAISIDDTRSAKRV
                     MPTVNGKGWEFDVLLDTNGDFKRLMNVNIPPHVFVLNGKKEVVWQHVGYLDGDEAKYI
                     DVIRNVLAGKPVE"
     gene            17180..>18349
                     /locus_tag="PK345_04230"
     CDS             17180..>18349
                     /locus_tag="PK345_04230"
                     /inference="COORDINATES: protein motif:HMM:NF039629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6029 family protein"
                     /protein_id="HPX34263.1"
                     /translation="MKSIIHFSIFLLCITGPFVALSQGITEGAKVTGNIQIDGQYYTE
                     NETLGITDSSLNYRKTGMNGFANLLYTNGNFSAGMRMESYLNPMLGFDSRYEGVGVPY
                     WFARYKTGSFEMTAGHFYEQFGSGLVLRSWENWALGYDNNLYGFNTSFSPIDGITLKG
                     LIGIQRYFWEPYEIGNRGIVKGFDADFDLNEIFEKMADCQTRIDIGGSFVSKYEKVPK
                     VTYVVDSKYFIDSVEWNKQTVYELKLPNNVGSWAARVNISNGGLNFYSEYAEKSNDPN
                     ATNGQIYKKGTALYSSLAYSTKGLGVFLSTKWIDNMSFKSKITETGTPPMLDINYLPA
                     ISKEHQYSLAALYPYATQPNGEFGFQGQIDYKIRKGTALGGKYGTSLTLNYSLAKS"
BASE COUNT         6106 a         3268 c         3317 g         5658 t
ORIGIN      
        1 tttcaatggt actcataatg agtatagttc aggatacctg accgaattga tggccgaagc
       61 taaaattgca aaagatgatc caagagtgtg gttttcgcaa ctttacggaa tgagtgatca
      121 tatcagcttc aatcttgcac ataacggata taatgtagtt aaatacctac catatggccc
      181 ggtgaagtat gtaattccat atttagtaag acgtgcggaa gaaaacacct cagtaaaagg
      241 ccaaacaggg cgagaattat cattgattaa aaaagagatt aaacgtagaa aaagatagtt
      301 ataacaccta ctgctatcta ttcttaaaaa ttaaaggtgt agtaagacgc ggattcttta
      361 atgaaaccaa ccacactcca ccaaaaatta atgatgcagc aatgatgtgt tgccacgaaa
      421 gaggattaga acttataaaa taggctatcc ctgttactat aataggctgt aaataaacat
      481 aatagctcac aacgcctgca tctaaatact taaggccgta aatagtaagg aggtaagcca
      541 tgaaagtggt caaaacaatc acaaaaaaga gtgaaaacca tgtattaatc gtaaagcctt
      601 caaaacttat atcaattgct gagtggagtg ttaatggtag ggaaaaaatt gtaccatata
      661 aaaaagccca tttcatcaca gtaattggcg aatatttttt catgatcggt ttgatgatta
      721 tcaggtaaaa agagtaagca agtgtgttga gtaaagccat taaatttcct gtaaatgtgt
      781 ttgagctaaa tgaaagttcg ccttggtaag taattagcaa aacggctcct gcagcgccca
      841 tgccaatacc aatgatcttt aggaaagtaa cattttcacg caataaaaaa gaggcaatta
      901 tcaatacaaa gataggattc gaaacgtgga taatagcaac atctaccggc gttgagagat
      961 ttaaaccaat aaaaaacaaa agctgattta aagttacacc caacaaagag gcaagtgcaa
     1021 tagtcttttt atctgcttta tccacctttt ccgaaggcaa cataaacgaa atcataaaaa
     1081 aaaggaggaa tgatccaaca acccttaaaa ataataactg ttcaggactg agataatcag
     1141 gcatcagtcc ttttgatatc caatagttag ctccaaaaat cattgccgcc agtagcaacg
     1201 ataaatgtgc ttttaatgat tttgttaaat tcataagggc aaaaatagca gtaagtcatc
     1261 ttgcggcaag atatttttat caataaattc agatctgctt aattattggt ttatctgatg
     1321 tccccctctt tattctcaat aatttattta gttttgcagc cttattaatg atatgattta
     1381 ataattgaaa ttttcgtaaa accgaaagag actccggttt taatttagct gatagacaat
     1441 tcatttattt taaatctttt acaatatgag aaaaataatt cttacggtaa tagctaccat
     1501 attttttggc gttggatttc actttactgc caatgcctgt actaattatc taatcacaaa
     1561 aggggcttct gcagatggtt caacaatgat ctcttactct gctgattcgc acgtactcta
     1621 tggcgagtta tatcactggc ctgccggaat ttggccggaa ggcacaatga tggacattgt
     1681 agaatgggat tcgggaaaat atttaggaaa aataaaacaa gcacgacaga catataatgt
     1741 agttggcaac ataaacgaat accagttagc cattggcgaa acaactttta caggtctgga
     1801 aatgctcgaa agtcaggagg ggtctcttat tgattatgga agcctgattt atataacatt
     1861 gcaacgtgca aaaactgctc gcgaagcaat taaagttttt catgagctta cctccacata
     1921 tggttatcac agtactggag agtctttttc aattgcagac cccaacgaag tatggatact
     1981 cgaaatgatt ggcaaaggaa atggctataa aggagccgtg tgggttgcaa gaatggtacc
     2041 cgatggctac gtttccgctc atgccaatca ggcaaggatt accacattcc cacttgccga
     2101 tggcaaaata tcagttacag agaaagactt tgacaagttt atgaagaatt ctgcaatcac
     2161 tactatttac tctaatgacg tgatcacctt tgcacgcgaa aaaggcctat tcaaagggga
     2221 ggacaaaaac ttcagctttt ctgataccta taatcccgtt gactttggtg gcgcccgttt
     2281 ttgcgaaatg cgtgtatgga ctatgttcaa cagggtaact actggcatgg atcaatattt
     2341 tgattatgtg aaagggaaaa tcagacacga tgataaattg gcaaatggcg atgtaaatga
     2401 aaaccgatat gccactaaca gaatgcctct ttggattaaa cctgataaga aggttaagtt
     2461 agaagatatg ttcgagttta tgcgtgatca tcttcaagga accgaacttg atatgtctaa
     2521 agattttggc gctgagcctt ttggccttcc aatccgctgg agaccattga catgggaagt
     2581 tgatggtgta acttattgcc acgaaagagt tactgttact caacaaaccg gattttcttt
     2641 catcgctcaa atgcgtagct ggttacccaa tccaattggc gggattcttt ggtttggtgt
     2701 tgatgatgcc tcatgttcaa tctatatgcc tatgtattgt agcattacta aagctccata
     2761 ctcaatagaa agaggccatg gagcaatgat gacctggtcg gatgatgctg cattttgggt
     2821 ctttaatcaa gtaagcaatt ttgcttacac aagatggaat attatttatc ctgaaattct
     2881 cgaaaaaata cataactacg aacaagaatt tattataaat acttcaacca ttgatacaaa
     2941 ggcgcttgca ttgtttaaag aaaaccctga atcggctata aattacctta ccgattattc
     3001 aacacgcctg ggcgacaaag taacagatga ttggcgccat ttctaccgtt acctctttat
     3061 gaaatatatg gacggaaaca aaaaaactcc tattgatgga gagatgaacc caaaagtaga
     3121 atggccgggt taccctgaaa aatggaggcg aaaggttgtt gaagaaaccg gtgatcagtt
     3181 taaagtgatt gagtaataat aaaaacaccc taaatcctta ggataaagat atttgtaata
     3241 taataaatag ccgctttttg tcatttatta ataaacaata ttttatgtta gaaaaagaaa
     3301 aagaccggca aaaattgatt taccgctacg ctgctattgt ctttcttgtt ttgcttatca
     3361 ttatcgggat tctctacata attaagaccg ctaaaataag agagctaaat gccgaaaagg
     3421 aacaaataag aattgaccta cagagtgagt tagattcgtt gatgttgcag cacaatcata
     3481 ttaaaaaaga atatggcatt ttaactgaca gcctcgtggt taaagatagc ctaatacaag
     3541 ccaatgcaaa agaaattacc gaactgctca actataaatg ggagtattac caaatcaaaa
     3601 agaaattaga caggctgaga gtcgttgcac aaggatatgt ttatcagctc gattctcttt
     3661 atacagtgaa taaagagtta caggaggaaa atgaacgtat tcgcgaaaat taccaaaccg
     3721 aacggcagaa aaatatcggt cttacgaaag agaaacatga attgataaat aaagttaccg
     3781 aggctgccgt catgaaagcc tataatataa aatcaacagg tttgcgatac agaggagcac
     3841 gccaggaaga aaccgatcgc gcaagacgta ccgatgccgt aagaatatgc tttaccatcg
     3901 gcgaaaactc acttattacc cctggtaaaa aagatatcta tttacgaatt gcccgccccg
     3961 ataattttat actaatttat gacgatactg actactatac cttttttcat gaaggaaaca
     4021 aattgcagta ttccctaaaa catactattg actttaccgg gaaagcacaa aacgtttgtg
     4081 tagtctggaa caaacataat caggacgaaa aaacaatggc agggatatac catgtaatgg
     4141 tgtatgatga agaaaacctg attggagaaa cctcttttga actaaaatag ctgaaactct
     4201 ttattttaaa ataaaatcag ttggcagctt tcttttcttt tttgggataa gcgagttttt
     4261 caacccacca aacccacatc aaaaagatga caacgtaaaa aaacggtctg aaaagataat
     4321 cgtggctaaa atcccaatat tcaggccatt tggttattac tactgaaagg ccgatgatcc
     4381 ggaaaagatt agtaagatgc acagccaata ccccaagcgg gataaaccat aatttattct
     4441 ttaacgatcc ggtaaaaaac atcatcagaa taataaattg caaaattggc ttaaaacctg
     4501 aacaaccgcg atttacagaa atgtatccat tatttacaaa atacattgtc tttgtctgat
     4561 caatacaggt tatctcgatg tttaacacat tttccacaaa ccaggcgctg tggtaatata
     4621 caactttcga catgtttgat tcaaggttat ttatcgttgt ttttattggc caataatgca
     4681 tctttttaac ccaaaaccga tatgaaaaat gaacaacaac cgtaataaga ataaataaag
     4741 caacatcttt aaaaatgtaa agatgataac gtttgataaa actttttgcc tttgaaagga
     4801 tttcagatag atttttcatt ttttaaattt aatgaaataa attctcttta tcaaaacaat
     4861 taatttgatt tacaggaact taacaacctc attaaatgat tttctgactt tgttgccatg
     4921 cttcttaaaa tccttctcag gataacctaa tggcgttatt gcatatacta cttcaccctc
     4981 ttcaacaatg cctaactgat gtaacttttc ggtatcgaaa gcaactatcc aacatgtact
     5041 aactccaagc gattcggcct gaagaatcat gtgatccata gcaatagcaa tatccatttc
     5101 taacgagttg aatccatcac tactcctagt ccatgctaaa tctttttttc cagtaacaag
     5161 aagcaggtgc ggcgcctttt taaaccaaga tttatgataa caatcgcgga ccttatcaag
     5221 atattccggt gttgaaacca gataaaaacg ccaaggttgc cgatttgctg cagagggcgc
     5281 taaatgaccg gcttttaaaa ttttttgaat tttatcagat tcaacagggc gatgaggatc
     5341 ataactcctt acgctttcac gatttagtat taactcaaaa aaatccataa gtgatcaaat
     5401 tatttattgc aaaaatgcat tttttatcag atttgataaa tcaaagattg taaaaacctt
     5461 aaataaacat ttattcatga gttttaattt attttgagcg actaactcaa tagttaacta
     5521 ttgtttatca atgtattaat gtatttaaag ttattaatca aaagctattc aatttactaa
     5581 aaattggtaa aaagttcatc ttaaatgaga taaaataaaa taatatacct ttgcgcccgt
     5641 tttttttcac ctaaaacctt attatgaagt atttgtttgg attattcttt ttcgttttga
     5701 cagctattat tccgccctca tgcacattta atcatgatat agccggagag gatatgttta
     5761 acgtaaaaga tcaatcgctt aaccgaattc ttgaaagagg cactcttaga gcatccacag
     5821 tttataactc aatagattat tttatttatc gcagcgagcc tgccggttat caatacgaac
     5881 ttttaagttc atatgcaaga tatctgggtg ttaatcttga aattgtaaca aatcaagatg
     5941 taaattcagc ttttcaagac ctttggtcgg gacgggtaga tgttatagca caaggccttt
     6001 ctgttaccgg aaatagaaaa aaacatctcg atttcaccta tcctcttgct caaactcatc
     6061 aagtgctcat tcagcgcaaa gaagggtatt caaacaaatt tagtattgat ggtgttgccg
     6121 ccgattttat taactcaccg cttgatttgg ccgggaaaat catatttgtt ccgcaaggtt
     6181 catctgccgc aagccgatta agaaaccttc aagatgagat aggtggcaaa atatatgttg
     6241 tagaatatcc atacgatact gaaagtttga tcagcatggt ggccaccggc aaaattgatt
     6301 ataccgtatg cgacgataaa actgcctcag ttaatcaaaa gttttatcca catcttgaca
     6361 cttcggttcc ggttagtttt tcacaaaata ttgcctgggc ggtaaatatt ggttgcgaga
     6421 gtcttaaaaa aagcctggat gaatggcttg aggggtatct taattctgca aatgggcgat
     6481 ttgtgtacaa caagtatttt atttatccgc gttcgcatct ttccgaatat agttttacgc
     6541 cttcaaaaaa tggaaaattt tcgccatggg atgatttttt acgtagtaaa agcgccaatt
     6601 atgatttcga ctggcgttta gtagcctctt tgatttatga agaatcgcag tttcagccgg
     6661 atgctatttc tgtaaaagga gcatttggac ttatgcaatt tatgccagag acggccgaaa
     6721 agtataaaat caatttcgag tcttcacccg aagagcaaat tgaagcagga ttgaaatatc
     6781 tgagcgagtt agatagacaa tttaaaaaaa tgataaaaaa caaagaggag aggattaaat
     6841 ttgttttagc agcctacaat tccggtattg ctcacgtgtt agatgcccga cggcttgcaa
     6901 aaaaatatga tcgcaatcct aatttgtggg atgataatgt tgattatttt atgaaaaaca
     6961 aatcaaaccc agagtatttt actgattcag tagtacaaca tggttactcg aatggagtag
     7021 tttcatttaa cttcgtcaat gatatattaa atagatatga acattacaaa aatgtaattg
     7081 aataataggg aaacaccttg tattataaag aattaagcta ccctaattaa gagtggcttt
     7141 ttttattatt attgaaatat gatcccgaaa gattatcggc aaatttttct ttaatgactt
     7201 atcagacaat acatttgcaa aataaaggat tccatttttt taagattctt attttttgca
     7261 ttacaactgc ttgtaaaaat cgtttccttt atcatctaca attataaatg ccgggaaatt
     7321 cacaaccctt atttttctta ctgcttccat cccaagttct tcaaaatcaa ccagctctat
     7381 tgatttaata tattctttag caatgatagc agccggtccg ccgatagatc ctaaatagaa
     7441 tccgccatat tttgcacaag catcagtaac ctgcttgctt cgatttcctt ttgcaagcat
     7501 gatcatcgag ccacccatac tttgaaattt ttctacatac atatccatac ggccggcagt
     7561 tgtaggccca aaactacctg agggcaaacc cggtggagtt tttgccggac cggcataata
     7621 aataggatga tttttaaaat actcaggcat aggctttccc tcttctaata ttttgttgat
     7681 cctggcgtgg gcaacatcgc gtgcgactat tagtgtacca gataggctca accgtgtttt
     7741 aaccgggtat ttacttaatt cttccaatac ttcattcatt gggcggtcaa gattgatctc
     7801 cacagcaggt gaaagctccg gaggtgctgc aggcatataa cgctccggat ttttctccag
     7861 ttcttcgata aagatcccct cttcggtaat ctttgcttta atatttctat cagcactaca
     7921 acttacaccg attccaaccg ggcatgaagc agcatgacgc ggcagcctga taacacggac
     7981 atcatgtgca aaatatttcc ctccgaactg agctccaata ccactttcct gagttatttt
     8041 cttaatcctc tcctcccact caaggtcgcg gaaagcttga ccgtaaatat taccctgtgt
     8101 tggtaagttg tcataatatc cggtggaagc ttttttgaca acggcaagat tggcttcagc
     8161 gctcgatcca ccaataacaa tagcaagatg atagggaggg catgctgcag agcctaattc
     8221 actaatcttt tccttgaaaa atttagtaag gttttcttcg ttcagtaacg acttagtttg
     8281 ttggaataac aaacttttat tagctgaacc tcctcctttg gttacgataa ggaactcata
     8341 actatttcca gagcttgaat agatatctat ctgtgcgggt aaattggtag tggtgttttt
     8401 ctcttcggtc attgttagtg gagcaagtat agaatagcgc agataatttt gctgccatac
     8461 gtcaaaaata cctttagata agtgcatagc atcgtcaact ccggtataaa catcttctcc
     8521 tttttttgcc ataatgatag ctgtaccagt atcttggcat gttggcagct ttccttcggc
     8581 tgaaaccact tggtttttga tcattgtgta tgcaacaaaa cgttcgttat cgctagcctc
     8641 ggggtcttcg aggatggcgg ccaattgtcg caagtgcgag gaacgaagga agaaagagac
     8701 atcgaaaaag gcttcccggg caagtaattc aagcccttct ggttttactt ttaatatttt
     8761 acgtccatta aactcttcgg ttgaaacaaa atcttttgta atcaacctgt acttagttgt
     8821 gtcttcctta acaggaaaag gattttgata aacaaactca gccataatta taattgttta
     8881 atttgataat ttattgattt agcatcactt atctaatccc atagccatct taattttttt
     8941 tggtatatcg gtgatatgat aataaccgtt gaacatttca tgaccttgtc cgatagcaaa
     9001 aacgggtaca ggtgtgccag tgtgcgccca cgtagtccat cctaatccgg ctttatggtt
     9061 caaaatccgg gtagctgtaa cagcgatagg attttcattg ccataattaa gttgtttttc
     9121 catgtcacat atttggagtt cacctttcca acatttccat gcatcggata atagtttatt
     9181 ttcgtaatcg gataaagcta attcaccctc gccgcctaat ccaaaatatt cattaatcaa
     9241 tttaagaatt agttcataat tttgattctc aggtattttc aatgtagaat ccaataaatg
     9301 agcaaatttg ctaaaagaga ccttttgatg tatgagtagt aacaagtcag tttcgtaatt
     9361 ttttcctgac caacccaagg taatgcctcc tgtttcatga tcagcagtaa caatgataag
     9421 agtttcattc ggatgttctt tgtaaaactc caatgcacgc tctactgcat ccgatagtcc
     9481 tattatgttc caaattgccg ttgctccatc attggcatgt gctgcccagt cgtgatttcc
     9541 ttcttcagcc ataataaaaa accctttgtc attatcgaga agttcaatgg ctttatcaat
     9601 taaaaggctt aatgggatat cgtttctatt ctggtcaata acaaaatcct gtgatgctgc
     9661 ttttgttaga aaattactga tagcaatcac tctattatca ttccgtttca ggttatttaa
     9721 ttgatcaact cctcgtacga tatgatatcc agcttgtttg gcaagctgat aaaggtcggg
     9781 cttatcttta tctttacctg ttgcttgata aaaacctcca ccgccaaaaa aatcataatt
     9841 actgctgatc aagtcaagac caatttcata ataattgttt cggcttggtt gatgtgcata
     9901 aaacacagag ggtgttgcat ggttaatgct aacatttgaa ataataccca ctttatatcc
     9961 ttctctttta acaatactgg caatagagta aagattctga tctctcgtac cattcatgcc
    10021 gatagtattt atggtggttt tttgcccggt agatagtgca gtacctgcag cggctgagca
    10081 agtaattaac cggttaagcg cattattatt tgcaaaacct gtggtaggaa acttagtaaa
    10141 agagagtctt tcaaatccaa tttcgttttt ttgcgaagcc aaaaatgcct ccgttacagc
    10201 aacatgcgtc atacccatac catcgctaat aaaataaaat atatatttag cctgctgagc
    10261 atgtaaaaat ccagaataaa aaaagaaaaa gataactgga aaaattacaa tctttaaaat
    10321 acgatttgat gttatcatta aaaaagagtt tatcggtgta aaaaaattca gtttgtaatt
    10381 cgtcatttga aagtgttcaa aattcaattt attgtggctt ttaaaagtct atttgtatca
    10441 gtgagtaact attaataatt gtaactgtta gagacaaaaa tagtattctt tgcataacgg
    10501 aaatttagat ttgtttttcg aacgttaact ttttgacctg gaacataact catggtttca
    10561 gtttgttaca attgtattct cattttttag tagtgaacaa aagtttttgg tcaacattaa
    10621 cagattcaag tattttttta attttcactt gatcaataac aagtcttcta cgtaagagat
    10681 ctaaatctct gaatcgtttt tcatcgcggg tgtgttctac caggtagagt ctcagatttt
    10741 ctccatatat ctctttatta aaatcgaaaa tatttacttc gatattaaga ttatgttgat
    10801 ttaatgtcgg tcggatacca atattactca ttcctttata aatggttcct ttataatcaa
    10861 caagggtggc atatactcca taatagggta ttaatttctc cgggtcatca agatgaatat
    10921 ttgctgtagg gaatcctaat gttcgtccga gtctatttcc ttttaccaca aggccgctga
    10981 ttgaataata atatcctaaa aggttagaca ccaattgcat atcacctttt ttaatagcat
    11041 ctcgtattcg ggtactactg atagcaaatc cggcaagatc ttcagcttca atttgctcaa
    11101 cggcaaaacc taattttgcg gccatttgac ctaaaaaatg aatgtcgcct ctacgctctt
    11161 ttccgaactg atgatcgtat cctataatta gttttaccgg cttaaatact tcataaatat
    11221 acttttcaag aaaatattca gagctaaatt gtgcaaactc ttttgtaaaa tggataataa
    11281 aaagatgatc aacacctaat tgactaaaga tttgcacgcg ttcgtccatc gaaaggatca
    11341 gtctgagatc atatcctggt tgaactacta ttcgggggtg tggttcgaaa gtaattagaa
    11401 cgctttcccc ttcaattctt ttggccgctt caactaattt tgcaattact aattgatggc
    11461 caatatgaac gccatcgaaa gtaccgacgg taacaactgc attttttatt ttgccaatat
    11521 cagcaatatc tttataaatt ctcaccttaa aatatcaatg ttcttatcgg ctgacaaagt
    11581 taataaccta ttcaattctc aacacatcaa cgggattctt ttcagcaaaa acaaaataac
    11641 gaaacaaaag ccctgtaacc aacaagttat agggctttag gttgagtctc cccggaagga
    11701 ctcgaacctt cgacccgatg attaagagtc atctgctctg ccacctgagc tacggagaga
    11761 atcggccgca aaagtataaa aactataaaa agagtacttt tttgtataaa caaataagca
    11821 agtgcataaa aaaagctaaa agttagtggt attaggataa ccgtgaattt gaaatatccg
    11881 aaagaataat taaatttgat ggtcgaataa aatttctatt tttgccagcc aaattttaac
    11941 ctgaataata attaaatcac tacataaatt tttagaaatg aaattttatc gaagcaaatt
    12001 tttttatttg tcaatattag ttgttttgat aatcctattt tctacttctt gtgtaccgat
    12061 gaaaaaaatt aaatacttac aaagcgaagt aaaaaaagga gatgctgttc aaacaacttt
    12121 tgaacataaa aaatcagagg attttaagat acagcctggc gacaatcttt acatcagagt
    12181 aaataatgca ctttctacta ctgaaaatat tttttcctta gatcaagaat caaggtcgtc
    12241 caactattac aatgatgtag gtatttacct gaacagttat cgggtttctg actctggtta
    12301 cattgatttt ccatttgtcg gtaaagtgtt tttgaaagat catacgcttg aacaggctca
    12361 ggagctttta caatctatag tggatgatta tttaaaaggc actacagtta tcgtacgatt
    12421 ggccattttc aaggttacta tgcttggcga attcaatcgc cctggcgagt atagcgttta
    12481 cgagagcaag ttcaatattt ttgacgcaat agcacaagct ggcgatctga caacatttgc
    12541 caaacgaaac aagatttatt tggtaagaca aaccgaatct ggaagtaagg ttgttcatct
    12601 tgatttaaat gatatcggaa tattagaatc tgaatattat actattatgc ctaacgatat
    12661 catttatgct caaccggtta aaggtaaaaa ttttgcattt agagaattcc cttattctct
    12721 tatttttaca accatctcca caaccctttt acttatcaat tttttcaaaa caaattaaca
    12781 ttgtattaag tggaaacaac ccctaatata cacaacctga agcaagaaga gtcagttgac
    12841 ctgaaaatgc tctttattaa gtttttcaga tattggtact tttttgctct taccatattt
    12901 gttgcattgg taatagcatt tctcttcaac aaatacacaa agcctgttta tgaggtcaaa
    12961 accactgttt tagttaaaga tgaccgttca agcaaagacc tgattggcat tgggatttat
    13021 gacatgcaaa atttgcaaaa tgaaattggg atattgtcat cttattcttt agcagagcgg
    13081 actataaata atttaaattt tgaaatcacc tatcaacttg aggaaaattt cataacaaca
    13141 gagctttatg acaagactcc ttttatagtg gagatggata ctaatactcc tcagcttcaa
    13201 aatgtaaagt ttactgtttt aatactttca aatacacaat atcggttgga attgcaggta
    13261 gaggaaggaa tactgtatca ttttagcgaa agagaggaga aaggaaatat taccggtatt
    13321 aactttacac gtgattttaa ttttggagac caggtggaag gcgagtttgg gaaatttcgt
    13381 atttacctta cagaagaatt ttctggtgat tatttaaata aaaacttgat ttttatcttt
    13441 aatgactatc aatcgttgat ctcacaattc caaggtatta aggtagaacc tataaaacgt
    13501 gatgcctcta ttgtcgaaat ttctatccgc ggtagtaata aatgtaaaat gaaggactac
    13561 cttaatgagt taacttatca atatttgtat agaggtattg aaaagaaaaa ccagattgca
    13621 gataatacca tacgttttat tgattcagaa ttggttgaca tatctgattc attaactttt
    13681 gctgaaaccc gtttacaaga tttccgtgct tcgaatgagg ttatggatct ggattttaaa
    13741 gctaatcagg tgtttacaat aatgaggcag cttgaagatc aaaaggcaca gctcattgta
    13801 aaatccaaat attataaaaa tcttcaagac tatctgatta aacataaaga gagcattgac
    13861 gatattgtgg tgccctcatc aatgggaata gatgatccgc ttctgacaca acttatcacg
    13921 gaattggcaa agctttatac cgaacgcgcc caattgctat taaactcatc cgaacgtaac
    13981 ccttatgtgg tagcaataga ccaacgtatt gcaacaacaa agaacaccat ctccgaaaac
    14041 attcaaaaca taatcaatac atcttcgata tcaataaaag atattgatga aaggataggc
    14101 cgccttgaat tggaaatagg ccgcctgcct caaacacaaa gacaactatt cggtattgaa
    14161 aggacattta aattaaacga tgccatttac accttcctgc tgcaaaaacg ctcggaagca
    14221 caaattgcaa aagcctcgac tttacccgat aatgagatta ttgatgtagc acgaggcgga
    14281 gaccaagtat ttccaaaaaa aggccttaac tacctcattg cattaattct tggaatcgtt
    14341 ataccaataa tttacattat tgggaaagat tatatgaacg ataagattat tgaacgccag
    14401 gatgtggaaa aaataaccaa tatcccaatt attggacatc ttatccacaa taacaaggag
    14461 acgcagcttg tagtaccaga atcgccaaaa tcatcaatat cagagtcgtt ccgatccgtc
    14521 aggacaaaca ttcaatacct tactaaaggt aaaagtaaac aaaccgtatt agtaacatct
    14581 gatatggtag gagcaggaaa aacctttgca gctatgaact tggcagcaat ctttgctatg
    14641 tacgaaaaaa agactttact actcggattc gacctcagaa agccaaagat ttatcaagat
    14701 tttggattaa ccagtacgga aggaatcagc tcttatctga tcaacaaaag cccacttgaa
    14761 gatattatac aggtttcgcc ggtaaaaaat ctcgatatta taatggccgg gccagtgcca
    14821 ccaaatcctg ctgaactgat ctcttcagac aaaactgatg agctgtttga aagaattcaa
    14881 gagatgtacg actacattat cattgatacc ccacccgttg gccttgttac tgacgctttt
    14941 ctgcttatga aatatactga tgccaatctt ttcttggtac gccagaacta tactaacaaa
    15001 aaggttttcg agtccattat aaaagatatt gagcttagaa agttaccaaa catgtacatc
    15061 ctgatcaacg atgtaaaact tggtaaggga tcgtatggat atggttacgg gtatggctac
    15121 ggttacggct acggatatgg ttacggatat ggttacggat atggttacgg atattattcc
    15181 gacgatgacg aaacttctaa aaaatcattg ttaggtaaaa tattcggaaa agcgtgatat
    15241 gaaaaactaa tcatttagaa ttgttttttt ctaaatagct atgaaaagct attgtttccc
    15301 ctattaaaaa caggttagtt caatcatgat cggcatcgtg caaataaaga tatttgctca
    15361 acataagatc ctaaaaaata ctgcagtatt ttacaaaaac caaaactatt cacattttca
    15421 acagatctaa tagtattaca gaagagctga atcttctttt ttattttctt tgcaaattga
    15481 tgtaactgta atttaaattt aggaagatgg agctatcagt tgcaaaatgc aacaatttgt
    15541 tttagcacgt tgtttgtatc ttccacaaaa atccaaagct ttaaaatgaa caaactttat
    15601 cctttaaaat ttaaaccgct ttttaaagaa aaaatatggg gtggcacaag gataaaagat
    15661 atgcttggaa tagatttcgg gaacttgtcc aattgcggcg aagcttggat tttatcgggt
    15721 gttaagtaca atgaaacagt tgttacaaat ggatttctaa aaggtaatgg aataaatgag
    15781 cttgtagagg tctatatgga tgatttagtt ggtgaaaaga tatttgaaaa gcataaaaat
    15841 gagtttccta tacttgtcaa gctcattgat tccaacgact atttgagtat tcaagtacat
    15901 cccgatgata aattggctgc caaacggggt cttggaaatg gtaaaacgga aatgtggtat
    15961 attcttagcg ccgatccgga agctgagtta ataagtggtt ttaaagaaaa aattgaccaa
    16021 caaacctact tacaccatct tgaatcaaac actctaaaac agtttcttaa tactgaaaaa
    16081 gtgatggtag gcaatgtttt ttttatccct gccggtagaa ttcatgcttt aggccctgga
    16141 attttgcttg ctgagattca gcaaacctcc gacgttacct atcgtttgta tgattgggat
    16201 cgggtagatg gtgaaggcaa aaagcgggag ctacatacta aggaagctat tgaagctatt
    16261 gattttaatg tttataaaaa ctataaaacc caatactatg aggtgctgaa taaaagtgtc
    16321 gaactcgtta gtcaaccctt ttttacaacc aacataatac acattaatca gccaataaaa
    16381 cgagattaca ctatgctcga ttcgtttgtt atttgtactt gcgtacaagg aagctcagaa
    16441 atgatttatc ctgatggtaa agtgaatatt aacataggcg aatgtgtgct aatcccgaat
    16501 gagataaaag atatactgat taagcctttt agtgattgta agattttgga aacatttatt
    16561 atatgaatta tcttttttga aaattatagt tttgcaaaaa ttgttttatt ttttaattct
    16621 ttcaatatga aaaaaataat tcttttggtt ttagttgttg ttccatttac cactttgtgg
    16681 gctcaaggtg gatcgaaggc tctttttaaa cttcccgaag ccaaccttca gactatggat
    16741 ggaaagccct ttaatacaaa aaatcttcaa aatgatggca agcctatttt attaactttt
    16801 tgggcaacct ggtgcaagcc ttgtatgaaa gaacatgatg ccattaatga tctttacgat
    16861 gaatggatag aagaaaccgg tgtaaaaatt tatgccattt ccattgacga caccagatcg
    16921 gcaaaaaggg taatgcctac agtgaacgga aaaggctggg aatttgatgt tctgctcgat
    16981 accaacggtg atttcaagag attgatgaat gttaacattc cccctcatgt atttgtactc
    17041 aacggcaaaa aagaggtcgt atggcaacat gtcggctatt tagatggcga tgaggctaaa
    17101 tatattgatg taattagaaa cgtgcttgca ggcaagccgg ttgaataaat cctaaagtta
    17161 aacttttaag agattagttt tgaaatcaat aatacacttt tcaattttct tactgtgtat
    17221 aacgggtccg tttgttgcct tatcacaagg aataactgaa ggggcaaaag taaccgggaa
    17281 catacaaatt gacggacaat attataccga aaacgaaacg cttggaatca ctgattcatc
    17341 gcttaattat cgtaaaacag ggatgaatgg gtttgccaat ttgctttata ctaacggaaa
    17401 tttttcggct ggtatgcgga tggaaagcta tcttaatcca atgcttggat ttgattcccg
    17461 ttacgaaggc gtaggcgtgc catattggtt tgcaagatat aaaaccggat ccttcgagat
    17521 gactgcgggt catttctatg aacagtttgg cagtggactg gtattgcgta gctgggaaaa
    17581 ctgggcttta ggttacgata acaaccttta tggttttaat accagttttt caccaattga
    17641 cggtatcaca cttaaaggat taattggcat tcaacgctat ttttgggaac cttatgagat
    17701 aggtaacaga ggaattgtca aaggatttga tgccgacttt gacttaaatg aaatatttga
    17761 aaaaatggcc gactgtcaaa caagaattga cataggtggt agttttgtaa gcaaatatga
    17821 aaaggtgcca aaggttactt atgtagttga ttcaaaatat tttattgatt cggtagaatg
    17881 gaacaaacaa acggtttacg agctaaaact ccctaacaat gttggatcgt gggcagcaag
    17941 ggtaaacatt tccaatggtg gtttgaattt ttattcggaa tatgccgaaa agagtaatga
    18001 tcccaacgcc accaacggcc agatttataa aaaaggaaca gcgttatact catcgcttgc
    18061 ttattccact aaaggccttg gagtttttct ttccacaaaa tggattgaca acatgagctt
    18121 taaatcaaaa atcacagaaa caggtacccc cccaatgctc gatataaact accttccggc
    18181 catcagcaaa gagcatcaat atagtctggc agcgctttat ccatacgcca cacagcctaa
    18241 cggagagttt ggatttcaag gacagataga ttataaaatc agaaaaggca ctgcccttgg
    18301 tggtaaatat ggaacatcgc ttacgttgaa ttattcactg gccaaatct
//
LOCUS       DAOOAI010000039        18252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_4049_length_18252_cov_4.852393, whole genome shotgun sequence.
ACCESSION   DAOOAI010000039 DAOOAI010000000
VERSION     DAOOAI010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18252
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4049_length_18252_cov_4.852393"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..3103
                     /locus_tag="PK345_04235"
     CDS             <1..3103
                     /locus_tag="PK345_04235"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34264.1"
                     /translation="EVTLCLKAFGLEGCPETIDCLEININQSPMVSAGNHTTICENDV
                     VSLIGQSEHCSAIEWSSTGDGYFTDPANILTEYVPGQQDLINGFVEICLNGSGYSPCG
                     QNSDCIMISFVRLPIVFAGDDTTIGKNDLFVTEFATASNVSTLLWSTSGSGLFDNPEV
                     LVTTYTPSLEDIEDSEVILTLTAQPLTPCQIETTDELILKISYTCLDAVINSGGDGIV
                     CLGDDFMISDAQGMYYSGLLWETTGDGSFNDITVLNPIYTPGPQDEFYGAVTLCVTAY
                     GLEDCLDATDCLVLTIQKPPVAFAGNSNIVPFPEEGGYTFTTAWAEHTELVQWITTNG
                     MGIFDDENKINPTYQPSFLEIYYEYVTFGMVCSPINPCTVSDESFTNITFTLEGDDAS
                     VDINEEIIYFCENDTAIQINANGSYYSSVIWETTGDGYFRYGNSLSPIYVPGIIDSSG
                     IEFTLTVTAVAFPDYQSATDQVIVSKILPPEIIAGTDMTVCGDEPISIENFYAENVDL
                     VYWETSGDGDFTEPNNVITEYIAGQQDLINGYVVLTLYGINNNSICNYENASSSIIIT
                     FEWPVITTNIFDKDIKLGSPLELIIEANNAETIQWHGPNGIIEGTGSLILYINEATYE
                     DEGDYYCVITNQCTTIYSDTAHITVYEEQVINLPADWSGISSWIQPFEPEIESIFADI
                     LDDLILIKNMTQMYSPVLNIVMLETWNPQEGYEIKTKSAVDVVFTGNRNLNTTIEIKH
                     GWGYLPVISSCPVDVSSLFVGQQIEIIKEIGGMGTYWPQLGINTIVNLQPGRAYQIKA
                     IEDFNITFPECEANKSGNNGSKVYEPFNPTSWNNPVRTPSTHVIAIGKDAFYQLKPGN
                     VIGAFTGNGTCAGILQLTRNDEALVVFGKDPFTEVSDGFVSNEPINFYMFDPSCNETH
                     SIEVVFDKSFPNHDGLFHFNGMSCISGIKSSTFIAYENLTSAINIYPNPTSGEVTIEG
                     AQGKITVEVYQAGGLLLFKINEDNDNHKGRINFTLESYPAGIYYLRIISENVTDIRKI
                     IRW"
     gene            complement(3155..6292)
                     /locus_tag="PK345_04240"
     CDS             complement(3155..6292)
                     /locus_tag="PK345_04240"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HPX34265.1"
                     /translation="MKRYILLMLVSLFAIQMFASELVLIPTKSYEDSKNLFSNPALTI
                     NFYRDEFVIATLNGLPKHDIVILDSNPWESGMSYYVVYLDNNTDKLDYYNSINSVADV
                     LWDGGNILIIRIDEVAYGQLPPAKNDGLVRIFNNRVVLPRKMFYNTSGRFDPDPFIVS
                     LLNQVTGTNITATVQHLEDYTTRNCYKPQSIEAQNWLKEQYENLGLSVEIMDFPMPGG
                     AASDNVIATKLGTKYPDEFVVLGGHYDSISFSGAEPGADDNASGSAGVLEIARILSEY
                     EFDRSIIFCAFSGEEYGLYGSAAYASRCAQQGMDILGYFNMDMVGYLKPGNTTIMTSL
                     IYPQSALELANFYTSVTATYLPDFVVIPATFSGGDSDHTSFNQNGYMGIFPFEDVDNM
                     SPYIHTSNDLVGPSYNNENQAVVFTKAILASVATMANIKFPPRNLVGIPGDNKVELIW
                     DELLEIDEYRVYRDGSYIASSLTNGYIDLTVENGTLYQYYVTAIYSDTGEESIPSNVV
                     NVTPMPPIGLPLIIDFENGAPYWEFEGSWGITSTTSYSPSHSITESPSGNYANNIEII
                     AKLSPFNLNLGYTSASLSFWTKYNIEQNWDYMYLEISTNGTNWTSLATFTGVQNTWIE
                     KSYSLNNYLNKSNVFIRFRFKSDIMVNKDGMYIDDFKIDVTGGYTTQLMQIPQGWSGI
                     SSYLSPAQTQVNDLLEPLGSNLIILQDMTNVYWPEQNINTIGNWNTHNGYKIKLSNNA
                     TLQFLGSLEENKTLSLPQGWSLIPVISDCQVSCEELFGSYNQVFLVKDVAATDVFWPA
                     QNIQTLEKLIPGKAYYIYNSSPLTITFPSCSKTQSITLENQKVIKTQWEMVAPTGNSH
                     VISVPASAIQDFAVGDQIGVFTPADLCAGCLQIDDLTTNKALVVFANDSLTSAIDGFV
                     NNEPFKFKLFKNSSGKEYNLIATFDNSLPDYNVFVDEGISGLTKLIIDNTGIDMPKNI
                     TGVYPNPSKGNVVVKIKNGSNAILRILNLSGQCVLMQNIDKEAVINASSLTKGVYTFQ
                     FKGIDFNETQKVVIQ"
     gene            6586..6656
                     /locus_tag="PK345_04245"
     tRNA            6586..6656
                     /locus_tag="PK345_04245"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6618..6620,aa:Gln,seq:ttg)
     gene            6995..7609
                     /locus_tag="PK345_04250"
     CDS             6995..7609
                     /locus_tag="PK345_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007134449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPX34266.1"
                     /translation="MHIAVAGNIGSGKTTLTTLLSRHFKWEPHYESVDDNPYLASFYE
                     DMRRWSFNLQVYFLNSRFRQIVKIRESGKNVIQDRTIYEDAFIFAPNLHDMNLMSTRD
                     FDNYRSLFELMVSFIKPPELLIYLRASVPTLVNQIQKRGRDYEESIRLDYLKSLNDRY
                     EQWIEGYHDGPKLIFNVDQLDFAEKPEALGFIIDQIIAELHGLF"
     gene            complement(7616..8494)
                     /locus_tag="PK345_04255"
     CDS             complement(7616..8494)
                     /locus_tag="PK345_04255"
                     /inference="COORDINATES: protein motif:HMM:NF015818.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HPX34267.1"
                     /translation="MIPQQILNSLNDFFTKQLSHLKGDILQHSLSGGSINEAFRITKG
                     KERFFLKYNHADLYPGMFEKEAVGLKMLHTTGEIDVPEVIHFGNSGDYSFLLLTYIEQ
                     GKEKSNFWMEFAKQLAALHSHKAPQFGLDYDNYMGSLYQHNTFHNRWINFFIQERLER
                     QVRLARDRGNISSADVSAFERLYKKLEEIFPETKPSLVHGDLWSGNFMINENGLPSLI
                     DPAVYYGHPEVDIAMSTLFGGFADSFYETYNQSNPLEKGWQQRLDYYNLYPLMIHVNL
                     FGESYLRAVRRIIQKF"
     gene            complement(8529..9518)
                     /locus_tag="PK345_04260"
     CDS             complement(8529..9518)
                     /locus_tag="PK345_04260"
                     /inference="COORDINATES: protein motif:HMM:NF016338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPX34268.1"
                     /translation="MLNQQQINNIFKIASIDDFNETALEIFRYQLETNSIYRDFVTKL
                     RLQPEKVLSYHQIPFLPIDFFKNHQVICGSTTPKQWFESSRTTGITFSKHFYHNLTLY
                     EKSFLQNFRMNYGAPEDYCFLAMLPSYLESSRSSLVYMVGKLIETSKNLNSRFYKDNF
                     NEIAEALTQLDQKGQKTMLLGVSFALLDLVSDRSFKLKNTIIVETGGMKGRKREMVRD
                     ELHKRLKSGFGVDVIHSEYGMTELFSQAWSDGNGKFITPPWMKVLIRDINDPLSLLKD
                     GTTGGINIIDLANVHSCAFIATQDLGRKLHDGSFEVLGRFDNSDIRGCNLLMA"
     gene            9604..10122
                     /locus_tag="PK345_04265"
     CDS             9604..10122
                     /locus_tag="PK345_04265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34269.1"
                     /translation="MKTKFLFLILLSTLLFWFSSCVEKQVYPDIPSLEFKTFIKMTHP
                     AGYDSVGIIVLAYTDGDGDLGLDKKDTTSYNFFVTYYKMNNGVLSPGTIFNPVTQTYD
                     TIFFNNRFYDLAPPDYIGWIKGEIEDTIRPLYDPRSSKSRDTIMFQIYMTDRAGNKSN
                     IVETPIIVVQNP"
     gene            10233..>10511
                     /locus_tag="PK345_04270"
     CDS             10233..>10511
                     /locus_tag="PK345_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HPX34270.1"
                     /translation="MRYKAYESRKVLLKVFVLVTSIVFTIRLFYVQLIDERYVLSANN
                     NVLRYVTIYPARGTVYDRNGKLLIYNEAAYDLMVIPRLVKQIDTLEFCR"
     assembly_gap    10512..10521
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10522..12052
                     /locus_tag="PK345_04275"
     CDS             <10522..12052
                     /locus_tag="PK345_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HPX34271.1"
                     /translation="DITKEDFIKKMEKARNYSPFKESVFEPQINKESYGYIEEKLYKY
                     PGFYVQSRTLRKYPHATAAHSLGYIGEVSPQDIENNPYYKMGDYVGKSGIEKAYEADL
                     GGVKGLKIRMVDVFNREKGSYLDGRYDTAAISGNDITMTIDLDLQEYGEMLMKNKRGS
                     IVAIEPSTGEILALISSPSYDPNLLVGRIRKQNYPLLEADSLKPLFNRALLATYPPGS
                     TFKLAGSLIGLQEGVITPESRFSCSGKSSSPIKCSHDHYSPLALREAIQESCNPYFWG
                     VFKSVIDNPKYNSTEKTFRRWREYLLSFNFGKTFNSDLTVEVSGNVPSVEYYDKYFGK
                     NHWRSMTIRSLSIGQGELLITPLQMANMMAIIANRGFYYNPHIVKAINRENGIEYNRP
                     DKKVTMVESRHFQPVIDGMYLVYNSERGTARWYRDADIVMCGKTGTVQNPHGKDHSVF
                     TAFAPMDDPKIAIAVVVENAGFGSTYAAPIATLMMKKYLQGDFEKPYYEEIILEANMI
                     E"
     gene            12052..13314
                     /gene="rodA"
                     /locus_tag="PK345_04280"
     CDS             12052..13314
                     /gene="rodA"
                     /locus_tag="PK345_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HPX34272.1"
                     /translation="MPRSRSNITQTLDWPVVLIYLAMIFFGWVNIYAAVYNADHHSIF
                     DLTQSYGKQLIWIGSSILIALIILLFDAKFFSVFAYLIYWLTLLSLVVVLFFGKEISG
                     SKSWIEIGNFALQPSEFAKFGTSLALAKFLSDTNIDLKEFRQLIKAIIIIAIPVILIE
                     LQNDTGTALVFISFALVFYRQGMQGSILFFGFGIILLFFLTLLINNIVLIGILALIMV
                     VMLFQINRTPKNITTILAIFIFAVMFVFSVNYAFNNILKPHQQTRVNVLLGKETDLLG
                     AGYNVHQSLIAIGSGGLIGKGFLKGTQTKYNFVPEQSTDFIFCTIGEEWGFIGSVALV
                     AAFALLLIRIINMAERQRSSFSRIYGYCLFSVLFFHFAVNIAMTLGLFPVIGIPLPFF
                     SYGGSSLWAFTVFLFVFLRLDAERLNIL"
     gene            complement(13326..14789)
                     /locus_tag="PK345_04285"
     CDS             complement(13326..14789)
                     /locus_tag="PK345_04285"
                     /inference="COORDINATES: protein motif:HMM:NF024974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPX34273.1"
                     /translation="MSRFFQITVLGQTNLQTPEVDSISITADERPILSWFPNTDNTKY
                     YRIERLRPGGTGDSIGVAWGINNTSFTDTVSILSPCDSIIRYKIIARAPGENPYVSAW
                     SDELRTIWLQKPHLDICANTISLAWTGYINMPGDLGGYDIMASTDSVNYIVIGTNQPG
                     DTAFIHTDILPDKLYSYQIRAFNKDRTKTSSSCKKTLQSRTYPKPQSIKIKSVSVENN
                     DFIRVVWDIEPLVPILDFEVERSENNHFFEKIENITDPITYNPGRQYDDHKANFNAKS
                     YYYQITLSDSCGLNSNPIYVSKVHRTIFLQGEPHAGYINELHWNHYEGWPDGVDKYRI
                     YRDSGTGFALLTEVTANTDYFSDNVSTYASQGGVFSYFIEALENGNGQATSKSNQLTL
                     EMETKILAPNAFIPEALPPDNEFKPIVSFIAEESYELFIFNKWGGIIFRTKNPQEGWD
                     GKVDGKLQPDGAYVYIIKCLTPEGKPFEKKGTVTLIR"
     gene            complement(14855..16147)
                     /locus_tag="PK345_04290"
     CDS             complement(14855..16147)
                     /locus_tag="PK345_04290"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPX34274.1"
                     /translation="MHTNLFRKTEPPISAIYDINLQDPIHFNLDNGVDVWGINFGELE
                     VAKVDLLFDAGSVFGKNPLTSGFTNLCLREGTHNYTSDQIAEIIDNYGASIRLNSGRD
                     SAGITLVCLTKYLKALIPLLSDIVLKPTFPEKELKTLTDRYRQNFMVDMEKVKVLSYR
                     HFGKLAFGKNHIYNRYAKLEDYDNITPTDLMEFYEMFYKNSKFQIVISGLMPENIAEL
                     LNVYFGKHLIEENNIKPQGAYEAEPASGFHFFEKVNSLQSCINIGKPIFNIHHPDYIG
                     MQVLNTMLGGYFGSRLMKNIREDKGYTYGINSFFLNLKFSGLFKIVTQVGAEVTKPAI
                     DEIINEIERIRKAPAEEQELNLVKNYMLGNLLQLSDGSFGQSELLKTMLTFQKDISFY
                     KKMITTIKSISKEEIQDLAVNYLDPNSMLSIVVGKKTE"
     gene            complement(16171..17415)
                     /locus_tag="PK345_04295"
     CDS             complement(16171..17415)
                     /locus_tag="PK345_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HPX34275.1"
                     /translation="MIHFEKFVLPNGLRVIVHTDKYTSLAAISIIYDVGSRDENPDKT
                     GFAHLFEHLMFGGSINIPKYDEPLERAGGINNAYTTTDLTNYYLSLPVQNLEIGFWLE
                     SDRMFQLAFSEKSLETQRNVVTEEFRQTHLNQPYGDAWMLLRPLAYKVHPYRWPTIGK
                     EISHIQNAVMEDVKAFYNTFYNPANAVMAVAGNVKVDEVQSLAEKWFSSIKNNGENKR
                     NLPDEPKQTEPRFLEIERDVPANVLYKAWKMHKRTHPDYHSVDLLSDILGNGKSSRLY
                     RRLVMEKGIFTDIDCFITGNFDPGLFVITGRPSKGIKIKEAEKTLMDEIDIIQNEVPD
                     DIELQKVKNKLEARLTLSKVNVLNKAINLAFSEILGDAELINSEIDKYNAVSTYKIRD
                     VARRIIQPEQCSTLYYLVKNKQ"
     gene            17881..17968
                     /locus_tag="PK345_04300"
     tRNA            17881..17968
                     /locus_tag="PK345_04300"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:17915..17917,aa:Ser,seq:cga)
BASE COUNT         5517 a         3318 c         3564 g         5843 t
ORIGIN      
        1 agaggtaaca ctttgtttga aggcatttgg acttgaaggc tgtccggaaa ctattgattg
       61 ccttgaaatc aatatcaatc aatccccaat ggttagtgct ggaaatcata ccactatatg
      121 tgaaaatgat gttgtgtccc taattggcca atctgaacat tgcagcgcaa ttgaatggag
      181 tagcacggga gatggatatt tcactgaccc tgcgaacatc ttaacagaat acgtacccgg
      241 gcaacaggat ttaataaacg gatttgttga gatatgcctt aatggcagtg gctattctcc
      301 ttgtgggcag aattctgatt gcataatgat aagctttgtt agattaccga tagtatttgc
      361 cggtgatgat actaccatag gaaaaaatga tctttttgtt accgaatttg ctaccgcttc
      421 taatgtatca acccttttat ggtcaacatc gggaagtggt ctttttgata atcctgaagt
      481 actcgttaca acttatacac ccagtcttga ggatatcgag gattcggagg taattctcac
      541 ccttacagct caaccattga ctccttgcca gatagagaca accgatgaat tgattctcaa
      601 aatcagttat acatgccttg atgcagttat aaatagcgga ggtgatggta tagtttgcct
      661 tggtgacgat tttatgattt ctgatgcaca aggtatgtat tattcaggtt tactttggga
      721 aactactggt gatggctcgt ttaatgatat taccgttctt aatccaattt atacacccgg
      781 ccctcaggat gagttttacg gcgctgttac tttgtgtgtt acagcttatg gtttggaaga
      841 ttgtttagac gctactgatt gccttgtgct tacaattcaa aaacctccgg tagcatttgc
      901 gggaaatagt aatatcgtac ctttccccga ggaaggtggt tatacattta caacagcgtg
      961 ggcggaacat acagaacttg tacagtggat tacaactaat ggaatgggaa tttttgatga
     1021 tgaaaacaag ataaacccga cttatcaacc aagctttttg gaaatatatt atgagtatgt
     1081 cacctttggt atggtatgta gtccaattaa tccttgtacg gtaagtgatg agtcttttac
     1141 taacataact tttactcttg aaggcgatga tgcctcagtg gatataaacg aagagataat
     1201 ttatttctgt gaaaatgaca ctgcaataca aatcaatgct aatggaagtt actatagctc
     1261 tgtgatatgg gagacaaccg gcgatggtta ctttaggtat ggcaactccc tttcacccat
     1321 ctacgttcca ggaataattg atagctcagg aatcgaattt acattaactg taaccgctgt
     1381 agcatttcct gattatcaat cagcaactga tcaggtgatt gtttcaaaga tattaccgcc
     1441 tgagattata gccggaactg atatgacggt atgcggcgat gagccaatca gtatcgaaaa
     1501 tttttatgct gagaatgtgg atttagttta ctgggagaca agtggagatg gtgattttac
     1561 tgagcctaat aacgttatta cagaatacat agccggacag caggatttaa taaacggtta
     1621 tgtagtactt acactgtatg ggattaataa taatagtatt tgtaattatg aaaatgcttc
     1681 atcctcaata ataatcactt ttgaatggcc ggtaattaca accaacattt ttgataaaga
     1741 tataaaatta ggaagtccat tagaactaat tattgaggcc aataatgcag aaaccataca
     1801 atggcatggg cctaatggta ttatagaagg aactggcagc cttattcttt atatcaatga
     1861 agctacttat gaagatgaag gagattatta ttgcgttatt actaaccaat gcactaccat
     1921 ttatagtgat acagcacata ttacagttta tgaagaacaa gtaataaatc tgcctgccga
     1981 ctggagtggt atctcgtcat ggattcaacc ctttgaacca gaaattgaat ctatcttcgc
     2041 agatatactt gatgatctga tattaattaa gaatatgacc caaatgtata gtccagtact
     2101 aaatattgta atgcttgaga catggaatcc gcaggaaggg tacgaaatca aaaccaaatc
     2161 agctgttgat gttgtattta ccgggaacag gaacctaaat acaacgattg agataaaaca
     2221 tggatggggt tatcttcctg taattagtag ctgtccggta gatgtttcat cattgtttgt
     2281 tggtcagcag atagaaatta ttaaggagat aggaggtatg ggaacttatt ggccgcaatt
     2341 gggtataaat actattgtta atttacaacc tggcagggct tatcaaatta aggctattga
     2401 agattttaac attacattcc ctgaatgtga agccaacaag tcgggaaata atggctccaa
     2461 ggtttatgaa ccatttaatc caacctcttg gaacaatccg gttcgtaccc cttcaacaca
     2521 tgttattgcg atcggaaaag atgcatttta ccagctaaag cccggtaatg ttatcggagc
     2581 tttcacaggc aacggaacct gcgcaggtat tttacaactt accagaaatg atgaagcttt
     2641 ggttgttttt ggtaaagatc catttactga agtttctgac ggttttgtgt ctaatgagcc
     2701 gatcaacttt tacatgtttg atccctcttg taatgaaaca catagtattg aagtagtctt
     2761 tgataagagc tttcccaacc acgatggatt gtttcacttt aatggaatgt cgtgtatcag
     2821 cggtattaaa agctcaacat tcatagccta cgaaaatttg acctccgcaa taaacatata
     2881 tccaaatcct acttctggag aagttacaat tgaaggggct caaggtaaaa ttacagttga
     2941 ggtttaccaa gccggcggat tgttgttgtt taaaataaat gaggataacg ataaccacaa
     3001 aggcaggata aactttacat tagagagcta tcctgcagga atatattact tgagaataat
     3061 ctcggaaaat gttactgata tccgaaagat aatcagatgg taataaaaaa aaggccgctt
     3121 cgttttcgaa gcggcctttt ttagtgattt tagtttactg tatcactacc ttttgagttt
     3181 cattaaaatc aattccttta aactggaatg tgtaaacccc ttttgtcaat gatgaagcat
     3241 taattactgc ttccttatca atgttttgca ttagtacaca ttgcccagat aagttcagaa
     3301 ttcttaatat ggcgtttgaa ccatttttta ttttcacaac gacattgcct ttggaaggat
     3361 tggggtaaac gccggttatg tttttcggca tatctattcc agtgttgtca attattaatt
     3421 ttgttaaacc cgatatacct tcatcaacaa agacattata atcgggtaag gaattatcaa
     3481 aagttgcaat gaggttgtat tcttttcctg aagagttttt gaagagttta aatttgaaag
     3541 gttcattgtt tacaaatcca tcaattgctg aagttaatga atcattagca aagaccacaa
     3601 gtgccttatt tgttgtaaga tcatctattt gcaggcagcc ggcgcacaaa tcggcaggag
     3661 taaatacgcc aatctgatcg cctacggcaa aatcttgtat tgctgatgca gggacactga
     3721 taacatgaga gttccctgtc ggagcgacca tttcccattg agtttttatt accttttgat
     3781 tttccaaagt gatagattga gttttggagc acgatgggaa tgttatcgtt agaggtgagc
     3841 tattataaat gtaataagct tttccgggga taagcttttc tagggtttga atattttgtg
     3901 ccggccaaaa aacatctgtg gcagcgacat ctttaactaa aaatacctga ttgtaacttc
     3961 caaacagttc ctcgcacgac acctgacagt cggatattac aggtattaaa ctccagcctt
     4021 gaggtaaaga cagggtttta ttttcctcta aagagcctaa aaattgcaaa gtagcattgt
     4081 tggaaagttt tattttataa ccgttatgag tattccagtt accgatggtg ttgatatttt
     4141 gctctggcca atagacatta gtcatatctt gtaatattat gaggttgctt ccgagtggtt
     4201 ccagtaaatc atttacctgg gtttgtgccg gcgataggta gctcgatatt ccactccagc
     4261 cctgtgggat ctgcatcaat tgagtagtgt aaccgccggt tacatcaatt ttaaaatcgt
     4321 caatgtacat accgtcttta tttaccatta tatcgctttt aaagcgaaaa cggatgaaaa
     4381 cattggactt gttcaggtaa ttgtttaacg aatatgattt ctctatccat gtattttgta
     4441 caccagtaaa tgttgccaat gaagtccaat ttgtgccatt ggtcgaaatt tcgaggtaca
     4501 tataatccca attttgttca atattatatt ttgtccaaaa cgagagtgaa gccgaagtgt
     4561 aaccaaggtt taaattgaac ggactaagtt ttgcgataat ttcgatgttg tttgcataat
     4621 ttccagaagg actttcggta attgagtgtg agggcgagta cgaagttgta gaggtaatcc
     4681 cccagcttcc ttcaaattcc caatagggcg cgccgttttc gaaatcaata atcaatggaa
     4741 gtccaatggg tggcattggc gttacattga caacattgga tggaatagac tcttcgccgg
     4801 tatctgaata gattgcagta acgtaatact gatagagagt tccattctcg acagttaaat
     4861 ctatataacc attcgtaagg gatgaagcaa tataactacc atcgcgatag actctgtact
     4921 cgtcaatttc caacaattca tcccaaatca gttcgacttt gttatcgcca ggaataccca
     4981 ctaaatttcg gggcgggaat tttatattgg ccattgtggc taccgatgcg agtatagcct
     5041 ttgtaaatac cacagcctga ttttcattat tataacttgg tcctacaaga tcgttggatg
     5101 tatggatgta agggctcatg ttatctacat cttcaaaagg gaaaattccc atatatccat
     5161 tttggttaaa cgaagtatga tcactatcgc cgccactaaa ggtggctggt ataactacaa
     5221 aatcgggcag gtaggtagcg gtaactgaag tgtaaaaatt ggcaagctca agcgctgatt
     5281 gaggatagat caacgaagtc attattgtag tgttacccgg tttaagatag ccgaccatgt
     5341 ccatgttaaa ataaccaagg atatccatac cttgttgcgc acatcgggaa gcataagctg
     5401 cgctgccata caagccatat tcctcgcccg aaaaggcaca gaaaataatt gatctgtcaa
     5461 attcatactc gcttaagata cgtgcaatct caaggacgcc ggctgagcca gaagcattat
     5521 catcagcgcc gggttcagct cctgaaaagg atatcgaatc gtaatgaccg ccaagtacta
     5581 caaattcatc aggatatttt gttcctaatt tggtagcgat tacattgtcg cttgctgccc
     5641 cgccgggcat ggggaaatcc attatctcaa cagataaacc aaggttttca tattgttctt
     5701 ttagccagtt ttgcgcttca atagactgtg gtttataaca gttacgcgtg gtataatctt
     5761 ctaaatgctg aactgtagca gtgatatttg ttccagtaac ctgatttaac aatgatacga
     5821 taaaaggatc agggtcaaac cggccggaag tgttgtaaaa catcttacga ggtaaaacga
     5881 ccctgttatt gaatattctg acaagtccgt cgttttttgc cggagggagt tgaccataag
     5941 caacctcatc aattcgaata atgagaatat taccgccatc ccaaagcaca tcagccaccg
     6001 aatttatcga gttgtaataa tccaatttgt cggtattatt atcaagatac actacgtaat
     6061 aactcattcc actttcccaa gggtttgagt cgagaataac aatgtcatgc tttggaagac
     6121 cgttaagagt tgcaataaca aattcatcac ggtaaaaatt aatggttagc gcagggttgc
     6181 tgaacagatt cttgctatct tcataacttt tggtgggaat tagcaccagt tcggatgcaa
     6241 acatttgaat agcaaataag ctaacaagca ttagaagtat gtaacgtttc atagctaagt
     6301 gttttaatga aaaaagaaat aaatatttat tgcaaattta gacttttacc cgaaatacta
     6361 atttttgact ctcttttttt aaaactcgaa ctaaaaaatg atgaatcttt aagcctaaca
     6421 ttataaatgg tttgcatgaa atagatattt gattgctgtc taataaacat atttaatgtg
     6481 tttaaaaggt tctttgactg ttaataataa aaaaaaagaa attcaagaaa atatcgttct
     6541 atgcttgaaa agtgttactt ttgcagcctc gttttttgtt tagtatgccc gatggtgtaa
     6601 tggcagcaca agagattttg gttctcttgg tctaggttcg aatcctggtc gggcaacagc
     6661 aaacctattg ttaacgaata gattgacaat aggttttttt atttatgaaa agaatttttg
     6721 ggttgatgat ttattaaact gggtttaata gctaaagtct gacagttagt agttacaatg
     6781 aacaataaac ttttttaatt aaaatttacc taaaaataga agttggaaac ttaatcgtta
     6841 caatatgatc atatcatttg gttaaggttt tgacttgttt tagagaagaa aaatattaaa
     6901 tgatattgtt tttatttttc aaaactctca atcgaattga ggttcttttg tatttttacc
     6961 cctcaaatta cactaacatt aaaagcaaaa ttctatgcat attgcagttg caggtaacat
     7021 tggtagtgga aaaaccaccc tcacgacgct cctttcgaga cattttaaat gggagccaca
     7081 ttacgaatcg gtagatgaca atccttatct ggccagtttt tatgaagaca tgagacgatg
     7141 gtcatttaat ctacaggttt attttctcaa cagccgtttc aggcaaattg ttaagattag
     7201 ggaaagcggt aaaaatgtta ttcaggatcg aacgatttat gaggatgcct ttatttttgc
     7261 tcccaatcta cacgatatga acttgatgag cacccgcgat ttcgataatt atcgttcgtt
     7321 gtttgagctg atggttagtt ttatcaagcc gccggaacta ctaatctatc ttagagcatc
     7381 agttcctact ctggtaaatc agatacaaaa gcgtggtcgc gattacgagg aatctatccg
     7441 acttgattat ctgaagagtc tgaacgatcg ttacgagcaa tggatagagg ggtaccatga
     7501 tgggccgaag ttgatattca atgttgatca gttagatttt gccgagaagc ctgaagcatt
     7561 agggttcata attgatcaga tcattgccga gctacacggt cttttttaga gtgttttaaa
     7621 atttttggat aattctgcgt actgctcgga ggtagctttc tccaaacagg tttacatgga
     7681 tcatcagcgg gtaaagattg taataatcta agcgttgttg ccatcctttt tctaaaggat
     7741 ttgattggtt ataagtttcg taaaatgagt ctgcaaaccc gccaaatagt gttgacatgg
     7801 caatatccac ttcaggatga ccataataga ctgccggatc aatcaaagat gggagaccat
     7861 tttcattaat cataaaatta ccgctccaca gatcgccatg aacaagtgaa ggcttagttt
     7921 ctggaaatat ctcttccaat tttttatata aacgctcgaa agcggaaaca tcggcagaag
     7981 aaatatttcc tctatcgcgc gccagccgca cctgcctttc aagccgttct tggataaaaa
     8041 aattaatcca tctattatga aaagtattgt gttgatacaa cgatcccata taattatcat
     8101 aatccaatcc aaactgggga gccttgtggc tgtgcagtgc agcaagctgt ttagcaaatt
     8161 ccatccagaa atttgatttt tcttttccct gttcaatgta ggtcaacagc aaaaacgaat
     8221 aatcgccaga gttgccgaaa tgtatcactt ccggtacatc tatttcacct gtggtatgga
     8281 gcatttttaa accgacagcc tctttctcga acataccggg gtagaggtcg gcgtgattgt
     8341 attttaaaaa gaacctttct tttcctttgg ttattctaaa ggcttcgttg atagagccac
     8401 ctgaaagtga gtgttgtaaa atatctcctt tgagatgaga gagttgtttt gtaaagaaat
     8461 cattcaacga attgagaatc tgttgtggaa tcataagctc taatttttta atgtgagaat
     8521 aagtagcttt aagccattaa taaattgcaa cctctgatat cagaattatc aaaccggcca
     8581 agcacttcga atgacccatc atgcagtttc cggcctaaat cctgagttgc aataaaggca
     8641 caagaatgta cattggccag atcaataata ttgataccgc ctgtagttcc atcctttagt
     8701 aatgataaag gatcatttat atctctgatc agtactttca tccatggtgg agtaataaat
     8761 tttccatttc catccgacca cgcctgcgaa aataattcag tcatgccata ttccgaatga
     8821 ataacatcca ctccaaatcc agatttaagt ctcttatgca actcatctcg aaccatctct
     8881 ctctttcggc ctttcatccc accggtctcc acaataatgg tgtttttcag tttaaaagag
     8941 cgatcactaa ccaaatcaag aagagcaaag ctaacaccta aaagcatcgt tttttgccct
     9001 ttttgatcaa gctgagtgag ggcttcggca atttcattaa aattgtcttt gtaaaagcga
     9061 ctatttaaat tcttcgaagt ttctatgagc ttaccaacca tgtaaaccaa cgacgatcgc
     9121 gatgattcga gatatgaagg tagcatcgct aaaaaacagt aatcttcggg ggctccataa
     9181 ttcatccgaa agttttgcaa aaagcttttt tcatataaag tgaggttatg gtaaaaatgt
     9241 ttactgaaag ttatgcctgt tgtcctgcta ctctcaaacc actgtttggg tgttgtgctg
     9301 ccgcaaatta cttgatgatt tttaaaaaaa tctattggaa gaaatgggat ttgatgataa
     9361 gataaaacct tttcaggctg caaccgaagc tttgttacaa aatctctata aatgctattt
     9421 gtttcaagtt gataacgaaa tatctcaaga gctgtttcgt taaaatcatc aatagaagcg
     9481 attttgaaaa tattattaat ctgttgttga ttcagcattg gcaattagca attaacaatt
     9541 agcaattggc aattagtaat tagcaattgg taatttgtag ttaacaatta atatagttgg
     9601 tgtttgaaaa cgaaatttct ctttctgata ttgctgtcaa cgttgctgtt ttggtttagc
     9661 tcctgtgtcg aaaagcaggt gtatcctgat attccatcac ttgaatttaa aacttttatc
     9721 aagatgacac accctgcagg ttatgattca gttgggatca ttgtactggc ctataccgat
     9781 ggtgatggcg atttaggctt ggataaaaaa gacacgactt cgtataattt ttttgttact
     9841 tattataaaa tgaataatgg ggtattgagt ccgggaacga tttttaatcc tgttacacaa
     9901 acttacgata ctatcttttt caataatcgt ttttatgatc ttgctccacc tgattacatt
     9961 ggctggataa agggagagat cgaagataca ataaggcctc tatatgaccc tcgttcatcg
    10021 aaaagtcggg atacgataat gtttcaaatt tatatgacag accgtgcagg taacaaaagc
    10081 aatatcgtgg aaaccccgat tatagtagtg caaaatcctt aatgagccac aaatactttt
    10141 gtggaaaaat catttcgctg aaatcccgaa cctaataatc taaagcctaa ttttgtgtcg
    10201 taatatcaac tcatttttta ttttgttttg agatgaggta taaagcttac gagagcagga
    10261 aagttttact taaggttttt gttttggtaa caagcattgt ttttaccatc aggctttttt
    10321 atgtccagtt gattgatgag cgttatgtgt tgtcagccaa taacaatgta ttacgttatg
    10381 ttactatcta tcctgctcgc ggtactgttt acgatcgaaa tggtaaattg ctgatataca
    10441 atgaggcagc ttacgatctt atggtaatac cacgccttgt aaaacaaatt gacactcttg
    10501 aattttgccg tnnnnnnnnn nggatattac aaaagaggat tttattaaaa aaatggaaaa
    10561 agcacgtaat tattcacctt ttaaagagtc tgttttcgag cctcagatta acaaagagag
    10621 ctatggatac atagaagaaa agctatacaa atatcctggt ttttatgttc agtcgcgcac
    10681 tcttcgaaaa taccctcatg ctacggcagc acattcatta ggttacattg gcgaggtaag
    10741 ccctcaggat atcgaaaaca atccctatta taaaatgggc gattacgttg ggaagagtgg
    10801 catcgagaaa gcttacgaag ctgacttagg aggagtaaaa gggcttaaga ttaggatggt
    10861 agatgttttt aaccgcgaaa agggaagcta tcttgatggc cggtatgata ctgctgcaat
    10921 ttcaggcaat gatatcacta tgactataga tctcgattta caggaatatg gcgagatgct
    10981 aatgaagaac aaaagaggat caattgtggc tatcgagcct tcaaccggcg agatacttgc
    11041 gcttatctct tcaccctcat atgatcctaa tttgttggtt ggccgtattc gcaaacaaaa
    11101 ctaccctttg cttgaagccg attcgctaaa accacttttc aatcgagcat tattggccac
    11161 ctatccgcct ggatctacct tcaaattagc tggctcactt attggcttac aggaaggggt
    11221 gattactccg gaaagcaggt tttcatgcag cggcaaaagc tcaagtccta taaaatgtag
    11281 tcatgatcac tattctccgc ttgctctgcg agaggcaatt caggaatcgt gtaatcccta
    11341 tttctggggt gttttcaaaa gtgtgattga taatccaaaa tataattcca ccgaaaaaac
    11401 gttcagacgc tggcgagaat atcttctcag tttcaatttt ggcaaaactt ttaatagcga
    11461 cctcactgtg gaggtttctg gaaatgtgcc aagtgtagaa tactacgata aatattttgg
    11521 aaaaaaccac tggcgatcaa tgacgatacg ttcattatca atagggcagg gcgaactgct
    11581 aataactccg ttacaaatgg ccaatatgat ggctattatt gccaatcggg gtttttatta
    11641 taatcctcac attgtaaaag ctattaatag agaaaatgga atagagtata accggcccga
    11701 caaaaaggta acgatggtag aatcccgaca ttttcagcca gtaattgacg gtatgtattt
    11761 agtttataat agcgagagag gaacggcacg gtggtatagg gatgctgata ttgtcatgtg
    11821 tgggaaaacg ggtactgttc aaaatccaca cggtaaagat cattctgttt ttaccgcttt
    11881 tgcgccaatg gatgatccta aaattgctat tgccgtagta gtggaaaatg ccggatttgg
    11941 ttcaacttac gctgcaccta tagctacttt gatgatgaaa aaatatttac aaggcgattt
    12001 tgaaaagccc tattacgagg aaataatcct tgaagctaat atgattgaat aatgccaaga
    12061 tcaagaagca acataacaca aacacttgac tggccggtgg tattaattta tctcgctatg
    12121 atcttttttg gttgggtaaa tatttatgcc gctgtttaca atgctgatca tcatagtatt
    12181 tttgatttaa cccaaagtta cgggaaacaa ttgatctgga ttggatcttc aattttgatt
    12241 gcactgataa tcttattgtt tgacgcaaaa ttcttttctg tttttgccta ccttatttat
    12301 tggctaaccc ttctttcgtt ggtcgttgtt ctatttttcg ggaaagagat atcaggttcg
    12361 aaatcgtgga tagaaatagg gaattttgcc ttgcaaccct ctgagtttgc aaaatttggg
    12421 acaagtcttg ctttggcaaa atttcttagc gacaccaaca tagatttaaa agaattccgc
    12481 cagttgatta aagccataat tataattgcc atccctgtta tcttaattga actgcaaaat
    12541 gacacgggca cagcgttggt tttcatctcc tttgctttag ttttttatcg tcaggggatg
    12601 caggggtcta tacttttttt tggttttggg attatactcc tgttctttct tacactttta
    12661 atcaacaaca ttgtattaat aggtatattg gctcttatta tggttgtcat gctttttcaa
    12721 atcaatcgta ctccaaaaaa tatcactacc atcctcgcca ttttcatttt tgctgtaatg
    12781 tttgtcttta gtgtgaatta tgcctttaac aatatcctaa agccgcatca gcaaacccgt
    12841 gttaacgttc ttttgggaaa ggagaccgac ttgctcggtg ctggctacaa cgttcatcaa
    12901 tcacttattg caattggctc aggcggatta atagggaagg gattcctcaa aggaacccaa
    12961 accaagtaca attttgtacc ggaacagagt acagatttta tcttttgtac gattggtgaa
    13021 gagtggggtt ttataggtag tgtggcatta gtagctgcct ttgcacttct acttatcagg
    13081 attattaata tggccgaacg acagcggtca tcgttcagca ggatttatgg ttactgcctt
    13141 ttttcagtct tattctttca ttttgctgtc aacattgcga tgactttagg tttatttccg
    13201 gtaattggca ttcctcttcc tttttttagc tatggaggct catctctctg ggcgtttaca
    13261 gtatttctgt ttgtgttttt aagattagat gccgagagat tgaatatact ataatgatta
    13321 gtcgtttatc ggataagcgt taccgttcct tttttctcaa aaggcttacc ttcgggggtc
    13381 aaacatttaa tgatatagac ataagctcca tcaggttgta gcttgccatc aaccttacca
    13441 tcccaacctt cctgcggatt tttagttcga aaaataatcc caccccattt attaaatatg
    13501 aataattcat agctctcttc agcaataaaa gatacgatag gcttaaactc gttatcagga
    13561 gggagcgcct caggaataaa agcatttggc gctaaaattt tagtttccat ttcaagcgta
    13621 agctgattag atttgcttgt agcttgaccg ttgccatttt ctaaagcctc gataaaataa
    13681 ctaaaaacgc ctccctgact tgcatacgtt gatacattgt ccgaaaaata atctgtatta
    13741 gctgtaactt ccgttaaaag tgcaaacccc gttcctgaat cgcgatatat tctgtatttg
    13801 tcaacaccat ccggccagcc ctcataatga ttccaatgta gttcattaat gtagcctgca
    13861 tgaggctcgc cttgcaaaaa tatggtacgg tggacttttg aaacataaat tggattagaa
    13921 ttcagcccgc aactgtcgga taaggtaatt tgataataat aactttttgc attaaaatta
    13981 gctttatgat cgtcgtattg tctgcccgga ttatatgtaa tagggtcggt tatgttttct
    14041 attttttcaa aaaaatgatt gttttccgaa cgctctactt cgaaatcaag gataggcacc
    14101 aggggttcta tatcccaaac caccctgata aaatcattgt tttcaactga aacagatttg
    14161 atttttattg attgtggctt tggataagtt cgtgattgaa gtgttttttt acatgaggag
    14221 gatgtttttg ttctgtcttt attaaaagct cttatttgat atgagtatag tttgtcaggt
    14281 aatatatcgg tatgaatgaa agcggtatcg ccgggttggt ttgttccgat cactatatag
    14341 tttacgctat cagtagaggc cataatatca taacctccaa gatcgcccgg catattgata
    14401 tagcctgtcc atgctaaaga aatggtattg gcacagatgt caaggtgtgg cttttgaagc
    14461 caaatggttc tcaattcatc cgaccatgcc gacacataag gattttcgcc cggggcacga
    14521 gcaataatct tataccttat tattgaatcg caagggctaa gaatagaaac agtgtcagtg
    14581 aaagatgtat tatttattcc ccaagctacg ccaatagaat cgccggttcc acctggccgt
    14641 agtcgttcaa tccgataata tttagtgtta tcggtgtttg gaaaccatga gagtatagga
    14701 cgttcatcgg ctgttatact gattgagtca acttccggtg tctgaaggtt ggtttgccct
    14761 aagaccgtta tttgaaaaaa tctcgacaaa cataaaatac agattgcaaa tactttgcaa
    14821 acagggatta agcgttttga aaaacaggta ttcattactc agtctttttt ccaactacaa
    14881 tacttagcat tgaattggga tcaagataat taacggccaa atcctgaatt tcttctttag
    14941 aaatagattt tattgtagtt atcatttttt tgtaaaagct gatatctttc tgaaaagtaa
    15001 gcattgtttt aagtaactcg ctttgaccaa atgatccatc ggagagttgt aacaggtttc
    15061 cgagcatata attttttaca aggttgagtt cctgctcttc ggccggagct tttctgatac
    15121 gctcaatctc attgattatc tcatcaatag ctggcttagt aacttcagcg cccacttgtg
    15181 taacaatttt gaaaagtccg gaaaacttca gattaagaaa aaatgaatta atcccgtatg
    15241 tataaccttt gtcctcacgg atgtttttca taagtcttga gccgaaataa ccaccaagca
    15301 tagtgttcag tacctgcatg ccgatatagt caggatgatg tatgttaaaa atgggcttac
    15361 cgatattgat acatgactgc aacgaattga ccttttcaaa aaagtgaaac cctgatgctg
    15421 gctcagcctc ataagcgccc tgtggcttga tattattctc ctctatcaaa tgtttgccaa
    15481 aataaacgtt caacagttca gcaatatttt caggcatcaa gcccgaaatg acaatttgaa
    15541 atttggagtt tttgtaaaac atttcgtaaa attccatcaa gtcggtcgga gtgatattat
    15601 cataatcctc aagttttgca tagcgattat atatatggtt tttaccaaaa gccagtttac
    15661 cgaaatgccg ataggagagt acttttactt tttccatatc taccataaaa ttttgcctgt
    15721 aacgatctgt taaggttttt agctcttttt caggaaaggt aggttttagt actatatcgc
    15781 taagtagcgg gatgagcgct ttaagatatt tcgtcaggca aaccaaggtt ataccagcgc
    15841 tatccctacc actgttcaat ctgattgagg cgccatagtt gtcaattatc tcggctattt
    15901 ggtcggatgt ataattatga gtaccctctc taaggcaaag gtttgtaaaa ccggaagtta
    15961 atgggttttt gccaaacacc gaaccggcat caaaaagaag atcaaccttt gcaacttcaa
    16021 gttcgccaaa attaatcccc caaacatcaa ctccattatc caagttaaaa tgtattggat
    16081 cttgcaggtt aatatcataa atggcgctga taggcggttc ggtctttctg aataggttag
    16141 tatgcatctc ttttttaaaa tatttatcgg ttattgtttg ttcttaacaa gataatatag
    16201 tgtgctgcat tgttccggtt gaataattcg gcgggctaca tcccgaattt tataagtaga
    16261 tactgcatta tatttatcaa tctctgagtt tattagttcg gcatctccta aaatctcgct
    16321 aaatgcaaga ttgatggctt tgttaagaac attaaccttt gaaagggtaa gccgcgcttc
    16381 caacttattt tttacctttt gtaattctat gtcgtcaggt acctcgtttt ggataatatc
    16441 tatctcatcc attaatgttt tttcggcctc ctttattttt attccttttg acggcctacc
    16501 tgtaataaca aaaagacccg gatcaaaatt tccggtaata aaacagtcaa tgtcggtaaa
    16561 aattcctttt tccattacta aacgcctata taggcgtgat gattttccgt tcccaaggat
    16621 gtctgaaagt agatcaacag aatgataatc gggatgagtg cgcttgtgca ttttccatgc
    16681 cttatacagt acattggcag gcacatctct ttcaatttca agaaaccgcg gttctgtttg
    16741 ttttggctca tcaggtaaat ttcttttgtt ctctccgttg ttttttattg atgaaaacca
    16801 tttttctgcc agactttgta cctcgtcaac cttaacatta ccggcaacag ccatgacggc
    16861 gttggcaggg ttgtaaaaag tgttgtaaaa agcctttaca tcctccatta cagcattttg
    16921 tatatgactt atctctttac ctatggtagg ccagcggtaa ggatgtactt tataggcaag
    16981 tgggcgcaac aacatccagg catctccata tggctggtta agatgtgttt gtcgaaattc
    17041 ctcagtaaca acgttacgtt gtgtttctaa acttttctct gaaaaagcaa gttgaaacat
    17101 tctatctgac tctaaccaaa aaccgatttc taaattttgc accggtaaag agagataata
    17161 attggtgagg tcggtagtcg tataggcatt gttgatcccc ccagcccttt caagtggctc
    17221 gtcatatttt ggaatattta tagagcctcc gaacataaga tgctcgaaaa gatgagcaaa
    17281 accggttttg tcaggatttt catcgcgcga acctacatca taaataatac taattgcagc
    17341 taatgaagtg tatttatctg tatgaacaat cacacgaaga ccattgggga gcacaaactt
    17401 ttcaaagtga atcatgctaa caattctttt aataactatt tcttcaaaat gttctttgag
    17461 aacaaaatag agatttcagg agattttggt acaattcaaa actttgctca attaagcaaa
    17521 agtgatgccc tatcatgcaa attcttgttt atttatttaa taaatccaaa gctacaactt
    17581 aattgctaac aaacctcaaa agtactttgt tctttttaag tgtgtttacc gcagccagtt
    17641 tgacgattct cgacatgata atagtaagtc gtttacggaa tccgacctaa agcagaacct
    17701 cgatcttgtt ctggagggca gacctgtgcc tattgagcaa atacctatta tcgtattcag
    17761 tattaaatga taaaacaacc cgcagtttga aagcaattca gttatatcat aaaatgatca
    17821 tagacacaag aattgttatc cgatttatct tttgtactgc aaaaaaatta attttgcagc
    17881 ggagaaatgc cggagcggtc gaacggggca gactcgaaat ctgttggccg gcttagctgg
    17941 cccgggggtt cgaatccctc tttctccgca agtcaaaaaa aataccactt aaagggcgtt
    18001 ttatttcgat ggaacaacca aattttattt aagttgttcc tgaaaaataa aaataataag
    18061 acgaagcgtt ttgttgattt gacgatttcc cttttagtgg atcataaaat agaccctctt
    18121 cctccgcaaa aagggtgtaa atggtagtat atcataaact gtgtaagttt aaaagccaaa
    18181 gcggatgtgg agtaagtcgg aaaattaata acaaagtcat agtatggctt aaagccatgc
    18241 tatgcctgaa aa
//
LOCUS       DAOOAI010000040        18226 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_4057_length_18226_cov_5.015519, whole genome shotgun sequence.
ACCESSION   DAOOAI010000040 DAOOAI010000000
VERSION     DAOOAI010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18226)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18226)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18226
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4057_length_18226_cov_5.015519"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2148)
                     /gene="porU"
                     /locus_tag="PK345_04305"
     CDS             complement(<1..2148)
                     /gene="porU"
                     /locus_tag="PK345_04305"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HPX34276.1"
                     /translation="MLKKQLIFLSFFVFSICWITALKGQNTITPFTIKWNVPVEVNIS
                     EGEFFKYLNFDDARYYENDEFTPYFSKVIKIEGNPSSLSAQLQSPKFIAMESNELQWF
                     NSLHLLRESIDVRADIGLSRGESFAIVTLLPFRINPENGQIEKLLSGEIYLNYSQSFE
                     KEVLSSNFTNNSVLSSGNWFKIKIDRTGIFKVTYDQLAGMGMNVNSLKSANIRVFGNG
                     GGMLPESNSAPRFDDLQETAIEVFDGGDGIFNQGDYFVFYGIGPDVWNFVPILFRFAH
                     KKNLYDDWAYYFITVDKGTGKRVAAQPPSTLTANYISTSISDYTFHELDEVNLIKSGR
                     EWYGETFDLINTYDYNFSFPNLKKDVQQHIKIRTYAKSILTSSFVANINDNDQLVISI
                     GGTPDNPEGLYAKEGVGELKFYVTNDKINVGLKYNKPATNAVGWLDYIELNVTRENIF
                     TGGQMAFRDVWSKDYEKVTEFQLRNVNSGVTIWEVTDRHNVTSIQATLSGNMMSFTLP
                     TEESVREFIAFDGTEYYDVQPVGPVANQNLHAISNIDYVIVTHPDFLPEALRLADFHT
                     SYSDLNTYVVTPDLIYNEFSSGSMDPTAIRDMMRMLYERGKTGKKPRYLLLFGDASYD
                     PKERLSNNTNFIHTFQSVNSDHYVYSYATDDYFGFLDLNEGTGDNQMLDVGIGRFVVR
                     TLQEAKMAVDKTIHYATFSENYGPWRNVVTFVAD"
     gene            2451..3431
                     /locus_tag="PK345_04310"
     CDS             2451..3431
                     /locus_tag="PK345_04310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PorP/SprF family type IX secretion system
                     membrane protein"
                     /protein_id="HPX34277.1"
                     /translation="MIQLKKNIFSTAIFFLILFTASTQDFPYSQFYANPLYLNPALAG
                     SEYCSRINLNYRNQWPSLPGMFTSSSFSFDCFSEFLNGGLGLQLNYDKQGEVSINQLM
                     VSGMYAYRLTISPKVEANMALQATYGQRGLNLENMILPSGLQSNNQTPVVGIAEDVKY
                     IDFASGFLIGFNEKYFLGGAVHHPTQPEIGFLEEHVYKMKMKITLHGGANFGGEKYHS
                     RYSSSEIVVSPNILYQQQGNFKHINIGSYFTYEPLVVGLWLRHAFKNPDAVIFLLGLQ
                     HQKYRLGYSYDYTLSQIGAAAGSAHEISFSWIFACDKKSRRRGAIKCPSF"
     gene            3463..4956
                     /locus_tag="PK345_04315"
     CDS             3463..4956
                     /locus_tag="PK345_04315"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HPX34278.1"
                     /translation="MFKNQYLSFFAIIIFSLFLMPSCKGIFGSKNKVSPTTGWEYNNP
                     KNGGFEVSRYREQETGPGLVFIEGGTFVMGQNDEDIMLEYNNPPRRVTVRSFYMDETE
                     VTNLDYLEYLYWLQRVFVSYPEVYNNALPDTLVWRERLSYNEPLANLYLRHPAYHDYP
                     VVGVTWIQANDYCSWRSDRVNEMLLIRQGILSVNPDQEDYDNFNTDAYLKGQYDGLVN
                     KALIDLSPTGTGERRVRLEDGILLPKYRLPTEAEWEFAALGIVARYENVAERRIYPWD
                     GRMVRSTSDKRTYGQYLDNFKRGRGDYMGVAGSLNDGADITAPVGTYPPNDYGLYNMA
                     GNVAEWVADVYRPLSHKDVDDLSPFRGNVFQKLLMQDGVPAEKDSLGRMRYENISIND
                     AANRINYRQADNRDYLDGDRMSVIDQSKWRLTNDAAMIDTTIVMYDYGRSSMISNNSR
                     VVKGGSWRDGAYFLSPGVRRFMDERTATNYIGFRCAMDRVGGPITSK"
     gene            5114..6412
                     /gene="murF"
                     /locus_tag="PK345_04320"
     CDS             5114..6412
                     /gene="murF"
                     /locus_tag="PK345_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HPX34279.1"
                     /translation="MKNINTQSLYKIFCQHPAISIDSRSITSGSIFFALKGNNFDGNQ
                     FARQAIDNGASFAVVDDPLLKEQKQMLFTPNTLTALQDLARYHRRKLQIPVIGITGTN
                     GKTTTKELIGKVLSQKFNTLITKGNLNNHIGVPLTLLNICPDTQIAVIEMGANHQHEI
                     ANLCKIANPTHGLITNIGKAHLEGFGNFQGVINAKSELYTYLRENNGVVFINQDDSLL
                     ESLAYEIEKIGYSQKDNALVKGQIITEAPFLSVEIFIKETKFQIKSQLIGAYNINNIL
                     AAITVGSYFNVEPELIKSAVETYKPDNNRSQFIRTKNNQVIMDAYNANPSSMEVAISN
                     FSKFPGSNKVMILGDMLELGREANNEHLKIINLAISSGVEKIIFVGMIFKDLVKAMPI
                     ESYLEVDELQEHLKTNPLTDKTILIKGSRGIKLEKLLEIL"
     gene            6421..7494
                     /locus_tag="PK345_04325"
     CDS             6421..7494
                     /locus_tag="PK345_04325"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016273002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HPX34280.1"
                     /translation="MKNSETKSYRVVGLMSGTSLDGLDIAYCLFWEENNKWRFKIPVA
                     HTYDYPQIWKSRLRSAVSMSGQELVQFNNEYGRYLGELANTFILDNSLHPDLIASHGH
                     TVFHQPEAGLTFQIGSGAEITAVTGITVVCDFRSQDVALGGQGAPLVPIGDKMLFGEF
                     DFCLNLGGFSNISFEEDDVRKAFDICPVNIALNYLSQKLGVEFDKDGALASSGTFCNS
                     LFNALNGLPFYAVEGPKSLGWEWLQAQFLPLLDDDNSPVKDKLRTTIEHIAYQISLKT
                     NKISDGKILVTGGGAFNKFLINRIGDLNNNTLIIPEDKIINFKEALIFAFLGLLRYLN
                     KPNCLASVTAATTDHSSGAIYSL"
     gene            complement(7602..10805)
                     /locus_tag="PK345_04330"
     CDS             complement(7602..10805)
                     /locus_tag="PK345_04330"
                     /inference="COORDINATES: protein motif:HMM:NF022481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2723 domain-containing protein"
                     /protein_id="HPX34281.1"
                     /translation="MENYKKYNNLIGWSIWLIATIVYFSTIEPTASWWDCGEYIATSY
                     KLQVGHPPGAPFFQLVGRIFSLFAFGNVSKVALMINVMSALSSSFTILFLFWSITIIA
                     RKLALLNGSMTTGKMIAVFGSGVVGALAYTFSDSFWFSAIEGEVYALSSLFTAIVFWA
                     ILRWEAEEGKSHSSRWLLLIAYLIGLSIGVHLLSLLVIPAIVFVLYFKKYKPSLKGML
                     VAGVISIIILAFIMYAVIPEIVNLFGHTEILFVNTFRLPFNSGTIFLAILLVTLIVLF
                     IKVTTITNNTLRNVTIGLSLILFSLILAESTSAGDFFFRLLMGGLIAGGIYLFRHKKA
                     YLNLVALSFAFLLIGYSSFILIIIRANANTPINENSPDDAIGLLSYLNREQYGSIPLV
                     YGPYYNAKVIDYKDGSPLYIRDTKEGKYRIADLRKDSEPVHDPEFCTLFPRMWYNLKN
                     TQINEYKRWGKIKGVPVRSVNERGETEVIYKPTFAENLRYFFRYQVGFMYMRYFMWNF
                     SGRQNDIQGHGEVENGNWITGFNNLDAKRLGDQKDLPPSMQNWGNNKFYMLPLLLGLI
                     GLFFQFNRDYKNGIVITLLFIMTGLAIVVYLNQYPIQPRERDYAYVGSFYAFAIWIGL
                     GVYAIFNSLNKFLSQSLSAILTTIVTLVLVPGVMAKDGWNDHNRSGKYAALDFAINYL
                     ESCEPNAVIFTNGDNDTFPLWYAQEVEGVRPDIRVVNFMLASGEWYIHQKMRMVYDSP
                     PLRFTISRENYDKGVNNYVPYLDRISGRWELKEIIDFIANNDNRTRIQLQDGTYINYL
                     PTKNLKITLDADDLIRRGIVNEEKRDRIPQEISWDVNQKILYKNDLMMLDFIATNNWE
                     RPIYFTSPSTVEGVFDLTKYCHLEGLAYRFMPLLADHLMEGIGGINVDRCYDLLVNKA
                     RWGRLNEPNVYVDAESRRNSIMPKQNYLRLASALVDLNKMDSAVRVLDTCQKYFPNDK
                     IYYDMYTLPMVEYYYAAGALEKGNEVSTIILNNYEADLQYYAKLSNYFREYYSQETER
                     AFAILQHLASIARRYDQAPQADKIESSLNLILENFKI"
     gene            complement(10957..13164)
                     /locus_tag="PK345_04335"
     CDS             complement(10957..13164)
                     /locus_tag="PK345_04335"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HPX34282.1"
                     /translation="MEKIPAIFWLAPVVSVLALSFAYYFFKKMMEAPEGTDVMKKIAT
                     HVRKGARAYLRQQYKVVGIVFIIITVVFSIMAYGLGIQNPWVPFAFLTGGTFSGLAGY
                     FGMKAATFASSRVANACRTCLDGGLRLAFRSGAVLGLTVVGLVLLDISLWFIVLNLAI
                     PETTPGIKLLTITATMLTFGMGASTQALFARVGGGIYTKAADVGADLVGKIEANIPED
                     DPRNPATIADNVGDNVGDVAGMGADLFESFAGSILATSLLGAAAYLSYGEKIQINAVL
                     APLLIAAVGTLLSILGVFLVKTKEGATQKQLLRALGFGVNSSAFFIVIFSFVILYFLN
                     IPNYVGLWGSIVVGLLAGIGIGQSTEYYTASAYKPTKMIAASSHTGPATVIISGVGIG
                     LISTAIPLIIVVVAITLAYGFATPNLFSFDAESINYGLYGIGIASVGMLSTLGITLAT
                     DAYGPIADNAGGNAEMAHLGKEVRQRTDALDSLGNTTAATGKGFAIGSAALTALALLA
                     AYFEEVKMMFVNFLGRSDELINGVPAKDAQFIDFIYYYDIHLMNAKVLVGVMIGVMSV
                     FLFSGLTMNAVGRAAGKMVDEVRRQFREIKGIMEEKAEPDYARCVEISTKGAQREMLI
                     PSLLALFIPIFVGLIFGVPAVTGLLIGTISAGFALAIFMANAGGAWDNAKKYIEEGNY
                     GGKGSDDHKAAVIGDTVGDPFKDTSGPSLNILIKLMSMAAVVTAGVAVSYHLL"
     gene            complement(13509..14534)
                     /gene="ruvB"
                     /locus_tag="PK345_04340"
     CDS             complement(13509..14534)
                     /gene="ruvB"
                     /locus_tag="PK345_04340"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006743717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HPX34283.1"
                     /translation="MKNINLDATGSSLSAGEKEIEKVLRPTHFNDFAGQAQVVENLKV
                     FVQAAKLRNEALDHVLLHGPPGLGKTTLSYIIANELEVGIKVSSGPVLDKPGDLAGLL
                     TNLEKDDVLFIDEIHRLTPLVEEYLYSAMEDYKIDIMIETGPNARSVQISLNPFTLIG
                     ATTRSGLLTAPLRSRFGINLRLAYYDAVTLMGIVKRSASILKVDILDEAAAEIARRSR
                     GTPRIANLLLRRVRDFAQIKSDGTIKMPIVQLALEALNVDKYGLDEMDNKILSVIIDK
                     FGGGPVGLTTIATAVGEDPGTIEEVYEPFLIMEGYLMRTPRGREATEIAYHHLGKTKN
                     SEQGRLF"
     gene            complement(14798..15751)
                     /gene="trxB"
                     /locus_tag="PK345_04345"
     CDS             complement(14798..15751)
                     /gene="trxB"
                     /locus_tag="PK345_04345"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HPX34284.1"
                     /translation="MSDSIEKVKCLIIGSGPAGYTAAIYAARADLHPVMYQGMQPGGQ
                     LTITTEVENFPGYPKGIQGPQMMEEIKQQAERFNTDIRWGEIVEIDLSKRPFKAKADD
                     GKEILAETVIIATGASAKWLGLPSEQEYNGYGVSACATCDGYFYKDKVVAVVGGGDTA
                     AEEASYLAKLSKKVYVIHRRNELRASKAMQKRLLKLPNVEMVWDSIPKEITGKQEGFS
                     KAVTGVIVENVKTGEIKNIEIDGFFVAIGHQPNSNIFKGQLEMDDAGYLITAPDSTAT
                     NVPGVFAAGDIQDKIYRQAVTAAGSGCMAALEAERFLADQD"
     gene            complement(15906..16082)
                     /locus_tag="PK345_04350"
     CDS             complement(15906..16082)
                     /locus_tag="PK345_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34285.1"
                     /translation="MKAASQVGPNVSEVLSALYRNGVGFIGQMFEDVKRWMQHENYSN
                     PEVSTLLHTNVYSL"
     gene            16339..>18226
                     /locus_tag="PK345_04355"
     CDS             16339..>18226
                     /locus_tag="PK345_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate sugar
                     epimerase/dehydratase"
                     /protein_id="HPX34286.1"
                     /translation="MSIRKHLLKYSNRFISGYVVLVTDLIIVAFSFVAAYLIRFNFVI
                     TEISQWKLPLYLPIILIISAIAFIISRSYVGIIRHTSTDDVIRIFKAAGIVFLTVIFL
                     NFIIVLYGWPIKKVIPLSIIIIHTLTTLFLMIFTRLVAKIVWINLLKGGRDTINVIIY
                     GAGKTGILTKNTLNADVENIYNIVCFIDDNPGKIGKSIEGIPVLNPSAITPQYLKKKK
                     IKELIFAMQNIAPSKRREFADAFLQYNIVIKNVPPVNLWINGELQTKQIRNIKVEDLL
                     MREPIILEKNIVLEQNRDKIILVTGAAGSIGSEISRQLMHCNSKKLILLDQAETPLND
                     LYLNLKHTFTDFSDRAEVLLANVTNERRMEWVFDHFKPEIVYHAAAYKHVPMMEESPV
                     EAVRVNVFGTQTLSKAAIRHNVEKFVMISTDKAVKPTNVMGATKRIAEMFIQGLHEDN
                     QIKTKFITTRFGNVLGSNGSVIPLFQKQIEEGGPVTVTHPEITRYFMTIPEACQLVLE
                     AGAMGNGSEIFLFDMGNPVKIVDLARKMIRLSGLKPDKDIKIEYTGLRPGEKLFEELL
                     FTTENTLNTYHPRITIAQVSPTNHKFLENKLNDLEKTLTTNDNFKVVALLKEITKEHV
                     KMEYI"
BASE COUNT         5819 a         3707 c         3363 g         5337 t
ORIGIN      
        1 atctgctaca aaggttacta cattacgcca ggggccataa ttttcagaaa aggtggcata
       61 atgaattgtt ttatccacag ccattttggc ctcttgaaga gttctgacaa caaaacgtcc
      121 tatgccgaca tcgagcattt ggttatcgcc cgtaccctca tttaggtcaa gaaatccaaa
      181 ataatcgtca gttgcataag agtaaacata atgatcagag tttaccgatt gaaaggtatg
      241 aatgaaatta gtgttattgc tcaaacgctc tttcggatcg tatgaggcgt ctccaaaaag
      301 caaaagataa cggggttttt tacctgtctt acctctttcg tacaacatac gcatcatatc
      361 cctaatggct gtaggatcca ttgagcctga cgaaaattcg ttataaatca aatccggtgt
      421 taccacatac gtattcaaat cagaatatga tgtatgaaaa tctgccaacc gtaaagcctc
      481 cggtaaaaaa tcgggatgtg ttactatcac ataatcaata ttggaaatcg catggagatt
      541 ttggttagcg acaggcccaa caggctgcac atcgtaatat tccgttccgt cgaaagcaat
      601 aaactccctc actgattctt cagtaggtaa tgtaaagctc atcatgtttc ccgaaagagt
      661 agcctgaata cttgtaacat tgtgccgatc agtaacctcc cagatagtaa cacccgaatt
      721 tacatttcga agctgaaatt ccgttacctt ttcataatct ttcgaccata catcccgaaa
      781 tgccatttgg ccgccagtaa atatattttc acgcgttacg ttaagttcaa tgtaatcaag
      841 ccaccctacc gcattcgttg caggcttatt gtattttaat ccaacgttaa ttttatcgtt
      901 agtaacataa aattttagtt cgcctactcc ctcctttgca tatagtcctt cggggttatc
      961 cggcgtgccg ccgatggata taaccaattg gtcattatcg ttaatatttg ctacaaagga
     1021 gctggtaaga atagatttgg cataagttct tatcttaata tgttgttgta catctttttt
     1081 aaggttaggg aaagagaagt tgtaatcata ggtattgatc aaatcaaaag tttcaccata
     1141 ccactccctc cccgatttta tgaggtttac ctcatccagc tcatgaaacg tgtaatcgct
     1201 gattgaggtt gagatataat ttgctgtcaa tgttgatggc ggttgggcag ctacacgctt
     1261 tcccgtacct ttatcaactg ttataaagta ataagcccaa tcatcataaa ggtttttctt
     1321 atgcgcaaac cggaaaagga taggcacaaa gttccaaaca tcggggccaa taccgtaaaa
     1381 cacaaagtaa tcaccctggt taaatatccc atcaccgcca tcaaatactt caatcgccgt
     1441 ttcttgaaga tcatcaaaac gtggcgctga attgctttcg ggaagcattc ctcctccatt
     1501 gccaaatact cttatgttag ctgactttaa cgaattgaca ttcataccca ttcctgcaag
     1561 ttggtcatag gttactttaa aaataccggt ccggtcaatt ttgatcttaa accagttgcc
     1621 agacgacaat actgagttat ttgtgaaatt gctgcttaaa acctctttct caaatgactg
     1681 cgagtaattt aaataaattt cacctgaaag caacttttct atttgtccat tttcaggatt
     1741 tattcgaaaa ggtaaaagag taacaatcgc aaatgattct cctcttgaaa ggcctatatc
     1801 agccctaaca tcaatacttt ctctaagtaa atggagcgaa ttgaaccact gcagttcgtt
     1861 gctctccata gctatgaact taggactttg taattgagcc gataaggatg aaggatttcc
     1921 ctctatttta atcactttgc taaagtacgg ggtaaactcg tcattctcat aatatctggc
     1981 atcatcaaaa ttgagatatt taaaaaattc gccttccgag atatttacct caacgggtac
     2041 attccattta atggtaaacg gagtaatggt attttgacct ttcaaagccg taatccaaca
     2101 tattgaaaag acaaaaaatg aaagaaagat caattgtttt ttaagcatag gtgttatttt
     2161 tatcaaatag tgatcaacgt tttaccagtc tcatttgttc agcaataact tccggcactt
     2221 tctgcaattt catagcgctt tgaaaattta tcttctaaaa tagtatcttg aaaaacgaat
     2281 aaaaagaggc taatattgct gaatattttt tgttatcgtc tttggataat tattagagtt
     2341 gtttttgaaa aatttattgc ttaagccgtt aacaagtata ttaaaataaa atatcgttcc
     2401 tctaaatgag ttaatagaga gattaatagt tgcgtaaagg ttgcttttgg atgatacaat
     2461 tgaaaaaaaa cattttttct acagcaatat ttttcttgat cctatttacg gcaagcacac
     2521 aggattttcc ttactctcag ttttatgcca atccattata tctcaaccct gctcttgccg
     2581 gttctgaata ctgttcgagg ataaatttaa attatcgcaa ccaatggcca tcgctgcccg
     2641 ggatgtttac ctcctccagc ttttctttcg attgcttttc cgaatttctc aacggtggtt
     2701 taggtttaca gttaaattac gacaaacagg gggaggtctc tatcaaccag ttgatggttt
     2761 cgggaatgta tgcttaccgg cttaccatta gccccaaagt agaggcaaac atggcactac
     2821 aagctacata tggacaacgt gggttgaatc ttgaaaatat gattttgccg tctggcctgc
     2881 agagcaacaa tcaaacccca gtggtcggca tagctgagga tgttaaatat attgactttg
     2941 caagtggttt ccttatcggt tttaacgaaa aatattttct cggaggcgct gtacatcatc
     3001 ctacacaacc cgaaattgga tttttggagg agcatgttta caaaatgaaa atgaaaatca
     3061 ccctgcatgg aggtgcaaac tttggcggag aaaaatatca tagccgttat tcaagttcgg
     3121 aaatagtagt atctccgaac attttatacc aacaacaggg taattttaaa catattaata
     3181 taggcagcta ctttacctat gagcctttag tagtcgggct atggctgcgt catgccttta
     3241 aaaatcctga tgccgtaata tttttattag gtttgcagca ccaaaaatac cgtttaggtt
     3301 atagttatga ttatacattg tcccaaattg gagctgctgc aggtagcgcc catgaaatat
     3361 cttttagttg gattttcgct tgcgataaaa aaagtagaag aagaggggca ataaaatgtc
     3421 cgagttttta gttagttacg tgtttttata aaactgtcaa gcatgtttaa aaatcagtat
     3481 ttgtcctttt ttgcaataat cattttcagc ctgtttttaa tgccatcttg taaaggaatt
     3541 tttggctcga aaaacaaagt atcccctaca acaggctggg agtataacaa cccgaagaat
     3601 ggaggatttg aagtatctcg ttatcgtgag caggaaacag gccctggctt agtgttcatc
     3661 gaaggcggca cttttgtcat ggggcaaaat gatgaggata tcatgttgga gtataacaat
     3721 cccccccgac gggtaacagt tcgttctttt tatatggacg aaacggaagt tacaaacctc
     3781 gattacttag agtatcttta ttggttgcaa agagtatttg ttagttatcc ggaagtttat
     3841 aataacgcat tgcccgatac ccttgtctgg cgcgaacgcc tttcgtataa cgagccatta
     3901 gccaatctct accttcgaca tccggcctat catgactatc cggttgttgg tgtaacatgg
     3961 atccaagcca atgactattg ttcttggcgt tccgatcgtg tgaacgaaat gttgctcatt
     4021 cgtcaaggta ttctttctgt taatccagat caggaagact atgataactt caatacagat
     4081 gcatatctga aaggacaata cgacgggtta gttaacaagg cgttaattga tcttagtcca
     4141 accggaaccg gcgaacgacg tgtaaggtta gaagatggta tcttactacc gaaatatcgt
     4201 ctccctaccg aagcagaatg ggaattcgca gccttaggaa tagtggctcg ttacgaaaac
     4261 gtcgccgaac gaagaattta tccatgggat ggcagaatgg ttagaagcac ttcagacaaa
     4321 cgtacttatg gccaatatct tgacaatttc aaaagaggcc ggggagatta tatgggtgta
     4381 gcaggttcat tgaacgatgg cgccgatatc actgcaccgg ttggcacata tccgcctaac
     4441 gattacggac tttacaacat ggcaggaaat gtagccgaat gggtagctga tgtttatcgt
     4501 ccgcttagcc acaaggatgt agatgattta agccctttta gaggtaatgt atttcaaaaa
     4561 ttactaatgc aggacggcgt tcctgccgaa aaagacagcc ttggaagaat gcgttacgaa
     4621 aacataagta taaatgatgc cgcaaatcgt ataaactatc gccaggccga caatagagac
     4681 tatttggacg gagaccgaat gtcggtcatt gatcaatcga aatggcgatt aactaatgat
     4741 gcagctatga tagatacaac tatcgtaatg tacgattatg ggagatcttc gatgatcagt
     4801 aataattcga gagtagtaaa aggaggctca tggagagatg gcgcatattt tttaagcccc
     4861 ggtgtgagaa gatttatgga tgaaagaact gccactaatt atataggctt taggtgtgca
     4921 atggatcgtg ttggcggccc aataacatct aaataaatta acaaaattca aaaaatccgt
     4981 tacagtttaa agtaacggat ttttttattt cctttaatat gccctgaatt tatcaatgat
     5041 accaaccaaa aatatataac aactctcaag aaagaggttt tcatgtaaat ttgcagttta
     5101 tgaaaaacct cacatgaaaa atattaatac tcaatcgtta tataaaattt tttgtcagca
     5161 tcccgcaatt tccattgact caagaagtat tacttcggga agtatctttt ttgccctaaa
     5221 gggaaacaat tttgatggaa atcaatttgc acgccaggca attgacaatg gcgcatcgtt
     5281 tgccgtagtt gatgatcctt tacttaaaga acagaagcag atgctgttta cccccaacac
     5341 tctgactgca ctgcaggatc tggcaagata tcaccgccgg aaattgcaaa tcccggtaat
     5401 tggcataacc ggaactaatg gaaaaacaac caccaaagag ctgataggaa aagtattatc
     5461 ccaaaaattc aacacactga ttaccaaagg gaacctcaat aaccatattg gcgtccctct
     5521 tactctgcta aacatttgcc ctgatactca aattgctgtg attgaaatgg gcgctaatca
     5581 tcaacacgaa attgccaatc tttgtaagat agctaaccct actcatgggc ttatcacaaa
     5641 catcggtaaa gctcatttag agggattcgg caactttcaa ggggttatca atgccaaatc
     5701 agaactctat acatatttac gagaaaataa cggtgttgta tttataaatc aggatgacag
     5761 tttgcttgaa agcctggcct acgaaattga aaagatcggt tacagtcaaa aggataatgc
     5821 cttagtaaaa gggcaaataa ttactgaagc gccattcctg tcagtggaaa tatttatcaa
     5881 ggagactaag tttcaaataa aaagccaatt gattggggca tataatatta ataacatctt
     5941 agctgctatt acagtcggca gttattttaa tgttgaacca gagctgataa aaagcgccgt
     6001 tgaaacctat aaaccggata acaatcgttc gcaatttatc cgtaccaaaa ataatcaagt
     6061 gattatggat gcttacaatg caaatccatc aagtatggag gtagcaattt caaacttctc
     6121 aaaattcccg ggtagcaaca aagtaatgat actcggcgat atgcttgagc tggggcgcga
     6181 ggctaataat gagcatctga aaattattaa tttagctatt tcttcgggag ttgagaagat
     6241 tatctttgtt ggaatgattt ttaaagatct cgttaaagcg atgccaatag aaagttattt
     6301 agaagtggat gagttgcagg aacatttaaa aacaaaccca ttaactgata aaacaattct
     6361 tattaaagga tcccgcggga taaaacttga aaaattattg gagatacttt aagaattaaa
     6421 atgaaaaata gtgaaacaaa atcgtacaga gttgttggtc ttatgtcggg cacgtcgctg
     6481 gatggcctcg atatagcata ttgtcttttt tgggaagaaa acaacaaatg gcgctttaag
     6541 attcctgtag cccatactta cgactatccg caaatctgga aaagcaggtt acgctctgcc
     6601 gtatctatga gcggacagga actcgtacaa ttcaataatg aatatggaag gtatcttggc
     6661 gaactggcaa atacttttat cctcgacaac agccttcacc ccgatcttat agcctcgcac
     6721 ggacacactg tttttcatca gcctgaagcc ggcctgacct ttcaaatcgg cagcggagct
     6781 gaaattaccg ctgtaacagg catcacagtt gtttgtgatt tcaggtcgca ggacgtagcc
     6841 ttaggcgggc aaggcgctcc attagtacct atcggagata aaatgctgtt tggtgaattt
     6901 gacttttgcc ttaacttagg agggttttca aatatttcat tcgaagaaga tgatgtaaga
     6961 aaagccttcg atatctgtcc ggtaaacatt gccttgaact atttatctca aaagcttggg
     7021 gtagaatttg ataaagatgg agcattagcc tcttccggaa ctttttgtaa cagcctcttt
     7081 aacgctttaa acggcttgcc attttatgct gtcgaaggcc cgaaatcgct cggttgggaa
     7141 tggctgcaag cacaatttct ccctttgctt gatgatgata attcacctgt aaaagacaaa
     7201 cttcgtacta cgatagagca tatcgcttat caaatttcac tgaaaacaaa taaaatatca
     7261 gacggaaaga tattagtaac tggcggcggc gcttttaaca aatttttgat caacagaatc
     7321 ggcgatctta ataataatac actgattatc cctgaagata agattataaa tttcaaggag
     7381 gcattaattt ttgcatttct cggtttgctg cgatacctca acaagccaaa ctgccttgcc
     7441 tccgtaaccg ccgccacaac cgatcattcg tcaggagcca tttattcgct ttagataatt
     7501 ccaaattaaa tgcttgtaat aagcacaata agaatttagt tgaaaaaacg ccaaatagaa
     7561 caaaattgcg gagaaaacgg ttgaatttag atttaggaaa cttaaatttt aaaattttcc
     7621 aatatcaaat tcaatgagct ttctatctta tcggcttggg gggcttggtc gtaacgccgt
     7681 gcaatggatg caagatgctg tagaatagca aatgcacgtt cggtctcttg cgaataatat
     7741 tcccggaagt aattactaag cttagcatag tactgaagat cagcttcata attattcaaa
     7801 ataatagttg agacctcatt acctttttcc agcgctcccg ctgcgtagta atattccacc
     7861 attggcaagg tgtacatatc gtaatatatc ttgtcgttag gaaagtattt ctgacaggta
     7921 tcgagtaccc tgaccgcaga atccatttta ttgagatcaa caagtgcaga tgcaagtcgt
     7981 aaataattct gttttggcat aatggaattt cgacggcttt cagcgtcaac atatacattg
     8041 ggttcgttaa gcctacccca gcgagcttta tttaccaaca aatcatagca tcggtcaaca
     8101 tttatacctc cgatcccttc cataagatga tcggcaagaa gcggcataaa ccgataagca
     8161 agcccctcta aatggcaata tttggtcaaa tcaaatactc cttcaacagt agatggactg
     8221 gtaaaataga ttggtcgttc ccaattatta gttgcaataa aatcgagcat catcaagtca
     8281 ttcttatata ggattttttg atttacgtcc cacgagattt cctgaggtat tctgtctctt
     8341 ttttcttcgt ttacaattcc ccgtctgata agatcgtcag catctaaggt aatttttagg
     8401 tttttcgtag gaagataatt gatataggtt ccatcctgaa gctgtattct cgtgcggtta
     8461 tcgttatttg caataaaatc aataatctct tttaactccc acctgcccga aatacgatcg
     8521 aggtaaggca cataattgtt aaccccttta tcataatttt ctctactgat agtaaaccgc
     8581 aagggtggtg aatcatatac cattctcatc ttttggtgta tgtaccattc tcctgaggcg
     8641 agcataaaat ttaccactcg gatatcgggt ctcactcctt ccacctcttg tgcataccaa
     8701 agagggaaag tatcgttgtc gccattagta aaaataacag catttggttc gcacgattcc
     8761 aaataattaa ttgcaaaatc gagagctgca tacttgccag agcgattatg gtcgttccaa
     8821 ccatctttag ccataactcc tggaactaac actaaagtaa caatagtggt aagtattgcc
     8881 gatagagatt gactaagaaa tttattcaac gaattaaaaa tagcatatac tccaaggcca
     8941 atccagatgg caaatgcata gaaagagccg acataggcat aatcacgctc acggggttga
     9001 attggatatt ggtttaaata gacaacaatg gctaagccgg tcataataaa gagcaaggtt
     9061 atcacaattc catttttata atcccggttg aactgaaaaa acagaccgat aagtcccaat
     9121 agtaatggca acatataaaa cttattgtta ccccagtttt gcatgcttgg cggcaggtct
     9181 ttctgatctc caagtctttt agcatctaaa ttgttaaatc ctgttatcca atttccgttt
     9241 tccacttcac catgcccttg aatatcattt tgcctacccg aaaaattcca cataaaataa
     9301 cgcatataca taaaccccac ctgatagcgg aaaaaatatc tcaagttctc cgcgaatgta
     9361 ggtttgtaaa tcacctctgt ttcccctcgt tcattcacac ttctgacagg cacacctttt
     9421 attttccccc aacgtttgta ttcattgatc tgcgtatttt ttaagttgta ccacatcctc
     9481 ggaaacagtg tacagaattc gggatcgtga acaggctctg agtctttacg taaatcggca
     9541 attctatact tcccttcttt tgtatcgcgg atatacaaag ggctgccatc cttataatca
     9601 ataacttttg cattgtaata agggccatat accaaaggaa tactgccata ttgttcacgg
     9661 tttaaatagg ataaaagccc aatagcatca tcgggactat tttcatttat tggcgtattc
     9721 gcatttgctc tgatgattat caaaatgaaa cttgaataac ctattagtaa aaaagcgaag
     9781 ctaagcgcta ccaaattgag atatgctttc ttatgacgga agagataaat gccgcccgct
     9841 atcaaaccac ccattaacaa cctaaaaaag aaatcaccgg cggaggtgct ttcagccaga
     9901 ataagtgaaa aaagtattaa tgataatcct attgtaacat ttctaagagt gttgttagtt
     9961 atcgtggtaa ctttaataaa taatacaatt aaagtaacca gaagtattgc caggaaaata
    10021 gtacctgaat taaaaggtag cctgaaagta tttacaaaaa gtatttctgt gtggccaaat
    10081 agattaacaa tctcaggaat tacggcatac ataataaagg ccaggataat aatggatata
    10141 acgcctgcta ccaacatgcc tttaagcgaa ggcttgtatt ttttgaaata aagcacaaaa
    10201 actatagccg ggataactaa caaactgagc agatgcacgc caattgaaag tcctatcaaa
    10261 taggctatca gtaaaagcca tcgtgatgag tgcgattttc cctcttcggc ttcccatcgt
    10321 aggatagccc aaaatactat cgcggtaaat aaagaagaca acgcataaac ttcgccttcg
    10381 atagctgaaa accaaaatga atcactgaac gtatatgcca atgcacctac cacaccactt
    10441 ccaaaaacgg ctatcatttt tccggtagtc atgctaccat ttaacaatgc taacttacgc
    10501 gcaatgatgg ttatactcca aaaaagaaat aatatcgtaa agctgctaga tagagctgac
    10561 attacattaa tcatcaatgc aactttggaa acgttgccaa atgcaaataa agaaaaaatc
    10621 ctgccaacca actgaaaaaa cggagctccc ggcggatgcc ctacttggag tttatatgaa
    10681 gtagctatat actcgccaca atcccaccag cttgctgtag gctcaatagt tgaaaaatat
    10741 actatcgtag ctatcaacca aatactccat ccaatcagat tattatactt tttgtaattt
    10801 tccattaaat tattcttttt aaataaaata cgatggtgcg aagatagtaa ataataggtt
    10861 tttacttaaa atgatattat taatcatttg atacattaaa aaaaccgctc tatatcaagt
    10921 atttacagaa cggcttttac tatttttcaa caaatattaa agcaagtggt acgaaactgc
    10981 tactccagca gttactacgg cagccataga cataagtttg attagaatat taagacttgg
    11041 ccctgaggta tccttgaacg gatcgcccac ggtgtcgcca ataactgctg ctttgtgatc
    11101 gtccgaccct tttccgccgt agttcccctc ttcaatgtat ttctttgcat tgtcccatgc
    11161 gccgccggca tttgccatga agattgcaag agcaaaaccg gcgcttatgg tgccgattaa
    11221 caaaccggtt acagcgggca cgccaaatat caaaccgaca aatattggaa taaaaagagc
    11281 aagcaaggat ggaataagca tttctctctg agcgcctttt gttgatatct caacacaacg
    11341 tgcataatca ggttcagctt tttcttccat aatacccttt atttcacgaa actgacgacg
    11401 gacttcatca accatcttcc ctgctgcacg accaacagca ttcatagtaa ggccgctaaa
    11461 cagaaatact gacataaccc caatcattac acctaccaac actttggcat tcatcaaatg
    11521 aatatcataa taatatatga agtcaatgaa ctgggcatct ttagcaggaa cgccattgat
    11581 caactcatct gagcgcccca gaaaattcac aaacatcatc ttcacttctt cgaagtaagc
    11641 ggctaaaagc gcaagcgctg ttaaagcagc gcttcctatc gcaaatcctt tccctgttgc
    11701 agcagtagta tttcctaatg aatcaagcgc gtcagtacgc tggcgaacct ctttgccaag
    11761 gtgtgccatt tcggcattcc caccggcatt atcggcaatt gggccataag catcggtagc
    11821 taatgtgatc cctaaagtag aaagcatacc tactgatgca ataccaatac cataaaggcc
    11881 atagttaatg ctttcagcat caaaggaaaa tagatttgga gtggcaaaac cgtaggcaag
    11941 cgtaatagcg acaacaacga taattaacgg tattgcagta gaaataagac caatacctac
    12001 tccggatata ataaccgttg caggacctgt atggctactg gcagcaatca tctttgttgg
    12061 tttataggct gatgcagtat aatattcagt agattgccca atgccaatac ctgctaaaag
    12121 gccaacaact attgaccccc acagcccaac ataatttgga atattaagaa aatagagtat
    12181 tacaaatgaa aatattacaa tgaaaaatgc ggaagagttt acaccaaaac cgagggccct
    12241 tagtaattgc ttttgagtag cgccttcttt agtctttacc aaaaatacgc cgaggatgga
    12301 aagcaatgtt cccactgcgg caataagtaa cggagctaat acagcattta tttgaatttt
    12361 ttcgccataa gagaggtaag ctgcagcgcc taacagggag gtggcgagaa ttgatccggc
    12421 aaatgactca aaaagatcgg ctcccatacc agcgacatct cctacattat ctccaacatt
    12481 atcggcaatg gttgctgggt tacgagggtc gtcttcagga atattagctt caattttccc
    12541 aactaaatca gccccaacat cggctgcctt tgtataaata ccgcctccaa cacgtgcaaa
    12601 caaagcttgt gttgacgcac ccataccaaa agtaagcata gttgcagtaa ttgttaaaag
    12661 ctttattcca ggagtagttt ctggaatagc aaggttaaga actataaacc ataacgaaat
    12721 atcaagtaaa accaagccaa cgaccgtcaa acccaacacg gcgccacttc tgaaagcaag
    12781 acgcaaacca ccgtcaagac aagtacgaca agcattggca acacgagagg aagcaaaggt
    12841 tgccgctttc ataccaaagt aacctgctaa accagaaaag gtaccacccg ttaaaaatgc
    12901 aaaagggacc caaggatttt gtatccccaa accataagcc attatactga aaacaacagt
    12961 aatgataata aatacaatac ccaccacttt atattgctga cgcaaatatg cgcgcgcacc
    13021 cttacgcaca tgagtggcaa tttttttcat aacatccgta ccctccggcg cttccatcat
    13081 ttttttgaaa aagtaataag caaacgacag agcaagcacc gaaacaactg gcgctaacca
    13141 aaaaatagct ggaattttct ccataccgca aatgagttta attgatacat taaacaataa
    13201 atttattgct aacagcaaca aaagtaaaac ttatcgaaaa acaatttgcc ttattttaat
    13261 aagattttta aatttggaat aattctatct acgcaaatca atatctaatg agctgcaatc
    13321 tacttcatta taagtgaata gagccgacaa agattaaact caaatcaaat aaaaaaaatc
    13381 cttttggctg aactaatgat tatttctgtt gagaaatatt tgataaaata tttgaaaata
    13441 gcaactcgat caaacattct caagtgcttg atcaagttta aaaggataat attcttaata
    13501 atttggaatt agaacaatct tccttgttca gagttttttg tcttgcccaa atggtggtat
    13561 gctatttcgg tagcttctct acctcttggt gttcgcatca agtaaccttc cattatcaga
    13621 aatggctcat acacctcctc gatagttcct ggatcttcac caacagccgt tgctattgta
    13681 gtaagaccaa cagggccacc accaaatttg tctattataa ccgaaagaat tttattatcc
    13741 atttcatcta agccgtattt atcaacatta agcgcttcta aggcaagctg aacaataggc
    13801 atttttattg taccgtcgct ttttatttga gcaaagtcgc gcacacgtcg caaaagaaga
    13861 ttggcgatac gtggcgtacc acgactacgc ctggctattt cagcagcagc ttcatccaaa
    13921 atatcaactt ttaaaatact tgccgatcgt tttactattc ccataagagt tactgcatcg
    13981 taataagcca aacgaagatt tattccgaag cgcgaccgta aaggcgcagt aagcaatcct
    14041 gagcgggtgg ttgcaccaat caaggtaaaa gggtttaaag atatctgcac actacgggca
    14101 ttagggcctg tttcgatcat aatatcaatt ttgtaatcct ccatggcaga atagagatac
    14161 tcttccacaa gtggtgttag gcgatgaatt tcatcaatga aaaggacgtc atctttttcc
    14221 aaattggtaa gtaatccggc taaatcgccc ggtttatcca atacagggcc tgatgacact
    14281 tttattccca cctcaagttc attggcaata atgtaggaga gggtagtttt acctaatcct
    14341 ggcggcccat gcaaaagcac atgatcaagc gcttcgtttc gtagcttagc agcttgtaca
    14401 aacactttca ggttctccac cacctgtgct tgtcctgcaa agtcgttaaa atgtgtgggc
    14461 cgaagcactt tttcaatctc tttctcgcct gctgaaaggg aagatccagt agcatcaaga
    14521 ttaatatttt tcatcaattg atatttttca caaaagtaga aaaatatcat catcatttgt
    14581 ttaagactcg atagagatga aagcgaaaaa gctgctttga ttagaagcct tttcgaataa
    14641 gtcaaaattt ttatacatta acccgaatta atcaaaataa agccctttcg gctggatcac
    14701 tggtatagtg atggtactca cgattaaata cgttaggtac tgttgtgttt attagaaaca
    14761 cttaatcatt tactcatcat caaacgctta agttaggtta atcctgatcg gctagaaaac
    14821 gctcggcttc aagagctgcc atacaacctg acccggcagc agttacagcc tgccggtata
    14881 tcttatcctg aatatctcct gctgcgaaca ctccaggcac gttagtagct gtagaatctg
    14941 gcgctgtgat aagatatccg gcgtcgtcca tttctaactg acctttaaaa atatttgaat
    15001 tgggctgatg tccgatagca acaaaaaagc catctatctc aatatttttg atctcgccgg
    15061 ttttaacatt ctctacgatt acccctgtaa ctgctttcga aaagccctcc tgtttaccgg
    15121 taatttcttt aggaatacta tcccacacca tttcgacatt aggaagtttt aaaagccgct
    15181 tttgcatagc cttagaagcc ctcagttcgt tgcggcgatg gataacatat acctttttac
    15241 tcaattttgc aagataggag gcctcttcag ctgcggtatc acctccacca acaacggcta
    15301 ctactttatc tttataaaaa tagccatcgc aagtagcgca agccgatact ccgtatccgt
    15361 tatactcctg ctccgatggc aaaccaagcc attttgccga tgcaccggta gcaataatta
    15421 ccgtctctgc caaaatttct ttcccgtcgt cagctttagc tttaaaagga cgctttgaaa
    15481 ggtctatttc aacaatctca ccccaacgta tgtcggtgtt aaaacgttcg gcctgctgtt
    15541 ttatctcctc catcatctgt ggtccctgaa tacctttagg atatcctggg aagttttcaa
    15601 cttctgttgt tatggttaat tgcccgccgg gctgcatacc ttgatacatc actggatgca
    15661 agtcggcacg tgctgcgtaa atagcagctg tataaccagc cggccccgat cctatgataa
    15721 gacactttac tttttcgatt gagtcgctca ttgaagaata tttttttgtt tactaatttt
    15781 tttcgcattg tcgaaaatca tgccaaaata aaccgcctta atatcacttt agaaagagta
    15841 acattaagat tgattaaatt aaacgaatag tgaatggtaa taatgcataa aatgtctaaa
    15901 gtttttcata aactgtaaac gttcgtgtgc aacagggttg aaacttcggg attggagtag
    15961 ttttcatgtt gcatccaacg tttaacatct tcaaacatct gacctataaa accgactcca
    16021 ttacggtata atgctgataa cacttctgaa acattagggc caacttgtga agcagctttc
    16081 attttttcaa cactatcatt taatccggaa tttccaacta taatgggctt acgaagtgtt
    16141 aagcttaaaa ttttagattt aatttttttg ttctttattt taactttaag gctccgtctt
    16201 tttattggct tatagcttaa gttacgtttt aggattttct ttttctgcgc tccaaaaaaa
    16261 ataattgtag ttttgtaaat caaactgaca aaatgccact tataataata taaatgtcaa
    16321 ccttatagaa tacaaaacat gagtattaga aaacatttac tcaaatattc caatcgtttt
    16381 atttcaggtt atgtagtttt ggttacagac ttgatcatcg ttgctttttc atttgttgca
    16441 gcttatctaa tccgtttcaa ttttgttatt acagaaatca gtcagtggaa attgccttta
    16501 tacctgccga taattttaat aatttccgct attgccttta ttatttcccg ttcgtatgta
    16561 gggattatcc ggcatacaag caccgatgat gtaatcagga ttttcaaagc tgccggcatc
    16621 gtatttttaa ctgttatttt tcttaatttt attattgtcc tgtacggttg gccaatcaaa
    16681 aaggtaatcc ctctcagtat cattatcata catactctta ctacattgtt tttaatgata
    16741 ttcacccggc ttgttgcaaa gatagtgtgg attaatcttt taaaaggggg tagagatact
    16801 attaatgtta tcatttatgg agctggaaaa accgggatcc ttacaaaaaa tactcttaat
    16861 gccgatgtgg aaaacatcta taatattgtt tgttttattg atgataatcc cggaaaaatt
    16921 ggtaagagca ttgaaggtat tcctgtttta aatccttcgg ccattacacc acaatatctg
    16981 aaaaagaaaa aaatcaagga gctgattttc gcaatgcaaa atattgctcc atcaaaaaga
    17041 cgtgagtttg ccgatgcttt tttacaatac aacatagtca ttaaaaatgt accgcctgta
    17101 aacctttgga tcaatggaga attacaaact aaacagatac gtaatatcaa ggttgaggac
    17161 cttctaatgc gcgagccaat tatattagag aaaaacatag tacttgaaca aaaccgggat
    17221 aaaattatcc tagttactgg cgcagcaggc agtataggga gcgaaatttc gcggcaattg
    17281 atgcactgca acagcaaaaa attaatcctt cttgatcagg ctgaaacgcc gctgaatgat
    17341 ctttatctta atctcaagca caccttcacc gacttttctg accgtgccga ggtgcttctt
    17401 gccaatgtaa ccaacgaaag gagaatggaa tgggtcttcg atcattttaa acctgaaata
    17461 gtatatcatg ctgctgcata caaacatgtg cctatgatgg aagaaagccc ggtagaagct
    17521 gtaagggtta atgtttttgg aactcaaaca ctctcaaaag cagcaatcag acataacgtt
    17581 gaaaagtttg taatgatctc gacagacaag gcggtaaaac ctaccaacgt gatgggcgca
    17641 acaaaacgga ttgccgagat gtttatccaa ggccttcatg aagataatca aataaagaca
    17701 aaatttatta ctacaaggtt tggaaacgta ttaggatcaa acggctccgt cattccactc
    17761 tttcaaaaac aaatagaaga gggtggcccg gtaacggtaa ctcatcctga aattacacga
    17821 tattttatga caattccaga ggcatgtcaa ttagttttag aagctggagc gatgggaaat
    17881 ggaagtgaaa tatttttatt cgatatggga aatcccgtaa aaattgttga ccttgcccgt
    17941 aaaatgataa gactgtcggg gttgaaaccc gacaaagaca tcaaaatcga atatacaggg
    18001 ctaagacctg gcgaaaagtt gttcgaagag cttttgttta ctaccgaaaa cactcttaac
    18061 acctaccatc ctcgtattac aatagcccaa gtaagcccaa caaaccataa gtttctcgaa
    18121 aataaactga atgatctgga aaagaccctg acaacaaatg ataatttcaa agttgttgca
    18181 ttattaaaag agatcaccaa agaacacgtt aaaatggaat atatcc
//
LOCUS       DAOOAI010000041        18039 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_4105_length_18039_cov_6.701902, whole genome shotgun sequence.
ACCESSION   DAOOAI010000041 DAOOAI010000000
VERSION     DAOOAI010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 18039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18039
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4105_length_18039_cov_6.701902"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     assembly_gap    132..231
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(273..710)
                     /locus_tag="PK345_04360"
     CDS             complement(273..710)
                     /locus_tag="PK345_04360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34287.1"
                     /translation="MKNLLLLIALMAGIYSVSAQGVEEYFQCVSCRSNAVNFGSYSSA
                     IGTNNTSTGTRSFAGGLNTQATGNYSFAFGEGALSTGINTFALGKDAKAGLMHSVAIG
                     FESEATSPSAFAIGYMNKAYAPSSYLFGENLKTLHSGAMIVAP"
     gene            complement(742..2238)
                     /locus_tag="PK345_04365"
     CDS             complement(742..2238)
                     /locus_tag="PK345_04365"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34288.1"
                     /translation="MKIYVIVLLTICLAVDGAAQHSFDFLYKTPDDKYIFNGSVDLEG
                     NAILVGRIGEYYYKGDGLVMKVQPDGKYMTKRFNKQDTLNRLCSVNILDNGNYMITGT
                     FGATDDWNYVDYFWVLILDHALEVIAENFFLIDEYYTGFGGYMKSLVDNDRNVCIVGQ
                     AIEFVTNPQSPRTDYVMFKLNQEGDTLMSRYYQYPYWQLPYELMIIPDTDSLMLLGGT
                     MSTAGHPTVTIMDKNMQIHAVNHFYISDLYDMLSADFWLNESEFLMAGPNSFSDGRYL
                     DDYIGVYRMNTAGQVFEELVLSIPDTSIYRAWHKSIAYANDSTVYVAGFQAFANNMTP
                     NAVYLFVIDREMNILGSTGFFIGDAQYQVTGILPMHDDGCLMHIAKNKPNAQGVLERD
                     VYIRKYLRENIVITTTVEKIPLEKLNLKLWPNPADAELFVSLEGMNPNQNIRFKIFNT
                     NGIMFLDKKLTINGNIIRCNISNMPAGLYVYEILGESMPKHTGKFIKK"
     gene            complement(2288..3253)
                     /locus_tag="PK345_04370"
     CDS             complement(2288..3253)
                     /locus_tag="PK345_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34289.1"
                     /translation="MMTAEDINVMNKIIAFREKLVYHRDNPYFKSGEVMSAEEARWNI
                     ETLFNVTYGFPYEKYGMVIADSAIIYLPVNSSGEVLLDDAISIYEDIIILVSQCYHSS
                     AFEDKGFLLLTIKTGEIVNGQIELVLLAVTGEKSAHDPNPPAQWEPFEDSANWWYGQN
                     KGKCNWSNPGTDAAKEIQNFLNTTKPIPPPPPTGYRYVYVTDTIIEKFGHEYEDENGN
                     HLIFYIEDENGNFSYDDYCLDSEELNFHYFGQREVIYTRIPQEENKQDNWVLFFCKLI
                     GKSGPSAQNPDYQCIRHENELEYAYRYIVPIEDIGEPVELEPPNN"
     gene            complement(4004..6760)
                     /locus_tag="PK345_04375"
     CDS             complement(4004..6760)
                     /locus_tag="PK345_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011108893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase/topoisomerase IV subunit A"
                     /protein_id="HPX34290.1"
                     /translation="MDEILKDHHEHEPVSANIYSNDHSGQFKASDGNYRITALDGMYE
                     NWFLDYASYVILERAVPDVTDGLKPVQRRILHAMYEMDDGRYNKVANIIGQTMKYHPH
                     GDASIGDALVQLGQKDLLIDTQGNWGNVLTGDGAAAPRYIEARLSKFALEVTFNPKTT
                     QWTSSYDGRNKEPILLPVKFPLLLAQGVEGIAVGLASKILPHNFNELIDASIKYLQGK
                     EFEILPDFPGGGLADFSKYNLGIRGGRIRVRARIKIEDKKNLTITEIPFGATTSSLID
                     SIIAANDKGKIKIKKIDDNTADKVEIQIQLQSGVSPDQTIDALYAFTNCEIAISPNSC
                     VIYNGKPHFMGVDEILKISADNTVNLLKMELEIRKSELEEQWHFSSLEKIFIEKRIYR
                     NIEECETWEAVITAINKGLTPYKNLFKREINKEDIIKLTEIKIKRISKYNSFKADEII
                     KNIELELEEVENHLRHLIDYAINYFKAIKKKYGPGKERCTEIRNFDTIEAKLVAATNE
                     KLYVNREEGFAGTGLKKDEYICDCSDIDDIIAFREDGTYMITKVSPKFFVGKSIIHID
                     VFKRNDKRTIYNAIYRDGKNGNTYVKRFAVTGVTRDREYNLTKGTPDSRVLYLSANPN
                     GEAEIVNVLLKPKPKLRKTSFDFDFSDLNIKGRNTQGNILTKNYVRKVTIKEEGISTL
                     GAMDIWFDDSVKRLNTEERGKYLNAFKGSDKIATLMQSGEYRLTGFDLSTHFDPDLIH
                     IEKYDPEKVFTAIYIEPKTGLHYLKRFTLEETDKKINFVSDNPEAKLVTISEHSRPNL
                     EVFFDHKRNEKQIDTQTINVEEFIAVKSYRAKGKRISNHFIKTVKWLEPLEDQDNETG
                     APTPDFDNDPVEDNDINEVKFTPEETPAIQPEVLKKPETAKIPEQHKSKSTSGEKPEQ
                     MSLF"
     gene            complement(6904..7446)
                     /locus_tag="PK345_04380"
     CDS             complement(6904..7446)
                     /locus_tag="PK345_04380"
                     /inference="COORDINATES: protein motif:HMM:NF013053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HPX34291.1"
                     /translation="MRILANESLGLIIDIQEKLYPFIKDNEQITRNTAILIEGLKAIG
                     VNIMVTEQYPKGLGFTIEPLKQLVSDIPVIEKTAFSCCDDQLFSKEFSKHQIKNVIIA
                     GIETHVCVLQTTIDLLKQGYQPVVIEDCVGSRSLNDKALAIERMRQEGAIISTYESIL
                     FELLRFSGTDVFKKISKLVK"
     gene            complement(7458..9503)
                     /locus_tag="PK345_04385"
     CDS             complement(7458..9503)
                     /locus_tag="PK345_04385"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HPX34292.1"
                     /translation="MEFVNPLFLYGLAAIAIPIVIHLFNFRRFRKVYFTNVRFLEELQ
                     QKTQKQSQIRHLLALLMRILAIASLALAFAQPYIPTSDASMKQQARNAVSIYIDNSFS
                     MGLTSSQGALLDEALGKAREVAMAYGTTDVFQLLTNDFQGVHQRFVNRQDFLQLLDEV
                     EISPARKMISEVMHRQADLFHENRYESRIACLISDFQKNITDVDQIKPDTTINGFLIP
                     VEAIEKSNLYIDSAWFDAPAFRLGQLIKLNVKIKSASDQDFEKVPVKLTINGQQRAIT
                     SFDLNAGKEIELELPFTIYESGIQYGTLEITDFPITYDDLLYFTFDVKELIPILAINA
                     DEESLYLNSLFQNDSSFFFQNEAENRLDYSSFSRYNLIILNGLNKISSGLTIELNKFA
                     ETGGSIAIFPGEEADFDSYRQLFISLNTSFLADKITSDTRVAGINTLSSIYDDVFESV
                     QENIDLPKVTSYYLIRRPTGSMMENQLDLLNGDIFMGSEPAVNGMLYLFAVPLNHEWS
                     NFVKHAMFVPTLYKIGLLSQGASVIYHTAGENERITINKSQSVGDQIFKIRSTNKDFE
                     VIPEIRSLFSQTEIFTQNQIKEAGHYKVVYNDETLAGLAFNYNRKESDLDFYSRADLA
                     KMVTDRGLTHFQVIKPTDKSLTEVIDELSAGIKLTKLFISLALAFLLGEILLLRFLK"
     gene            10165..10239
                     /locus_tag="PK345_04390"
                     /pseudo
     CDS             10165..10239
                     /locus_tag="PK345_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008632889.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein THX"
     gene            complement(10361..11749)
                     /gene="murD"
                     /locus_tag="PK345_04395"
     CDS             complement(10361..11749)
                     /gene="murD"
                     /locus_tag="PK345_04395"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HPX34293.1"
                     /translation="MKELLRRYIKDKKVLILGFGREGRSTFRLLQYFFPSLKMAIADR
                     NPGLPLTEVENEKVSELFLGKDYLNALGWADIVFKSPGINLGKSIDSSIKQKIVSQTS
                     LFLEKYSTQTIGITGTKGKSTTASLTYHILKEAGKEVVLVGNIGIPPFDLLQNISRKS
                     TIIFEISAHQLEYINHSPHIAVLLNIFQEHLDYFSSMDDYSSAKFNIAKYQQTNDFLI
                     FDNSNLIIKKLLSDYKYNGQKIAIQLSDKIYDAQNQTIHIAGLQEPICVKNSMLHGRH
                     NLKNIIITASILSKAGLQAKDIERGLPTFKPLEHRLEDLGVVADIRFINDSISTIPES
                     TIEAIHTLLDVDTIILGGHDRGVNYNKLVNLLADSAIRNLIFMGGAGERMMRLFAKKN
                     RPTKNLFFINRMSETEKIIVDYTQKDKICLLSPAAASYDQFNNFEERGRAFKAMINSI
                     KEKHELPDKDNP"
     gene            complement(11746..13122)
                     /locus_tag="PK345_04400"
     CDS             complement(11746..13122)
                     /locus_tag="PK345_04400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34294.1"
                     /translation="MLVQQNQSHFDNLRKNFTHLYYRGFSIETKNDHIRVKYHFNLSD
                     KYNFYPQLNIPLKSSSSKILNISFLENLSFQIGMIELISYWKAACPQKVVVDAGYLCD
                     FQIEWWKNVYFKGLGEFFYVNGIETNIHEFLNIEINSSKEYEMEEVEVGNRILIPIGG
                     GKDSAVTLELLKKSGFEIIPFILNPRAASLATAITSGFDQKQIFTIFRTLDPKLLELN
                     KQGFLNGHTPFSALLAFISLLAASLSGSRYIALSNESSANEPTIPGTQINHQYSKSID
                     FENDFRRYANQYLTQNIEYFSFLRPLNELQIGKLFAAIPDQHPHFKSCNVASKTDSWC
                     CKCPKCLFTWIILSPFMEEEKLVKIFGTNLFDDITLLPVLNQLTGQASEKPFECIGTT
                     DETNMALAMTIKKRYNTKLPALLKHYHQSVEAGKQNDPSFEIIMKELNRDNFVPIQFM
                     KILKEAIG"
     gene            13190..13780
                     /locus_tag="PK345_04405"
     CDS             13190..13780
                     /locus_tag="PK345_04405"
                     /inference="COORDINATES: protein motif:HMM:NF014659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HPX34295.1"
                     /translation="MENFDDLIKIKTNNTRPARGRILISEPFLYDYYFHRSVILLAEH
                     NKEGSFGVIINKPLAMGFGEVVKDFPDFNATVYLGGPVSADSLFFIHTLGVEIDGSME
                     IMEGLYWGGDLEQIKSYITIGRFNNDNIRFFIGYSGWSPNQLDSELQRNSWAVSALDA
                     DQIMKADPAGIWKSSLIDLGAEYSKWTNYPSDPGLN"
     gene            13789..15168
                     /locus_tag="PK345_04410"
     CDS             13789..15168
                     /locus_tag="PK345_04410"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease domain-containing protein"
                     /protein_id="HPX34296.1"
                     /translation="MKYAILDIETTGISYKNGKITEIAILIHDGKAIVDEFATLINPE
                     QKIPYYITQLTGINNRMVEEAPKFYEVAAKIVEITQDCTIVAHNASFDYNFLRHEFKA
                     LGYDFNRQKLCTVKLSRKLIPHQRSYSLSNLCRELNIPNLHPHRALGDAKATAVLFDI
                     LLKIDPNLSQLPFSGNNSSLKPEMINALPEKTGVYYFIDADQNIVYIGKSKNIRERVI
                     AHLTNSMTQRALEMKNKLAAVDWELTGNELIALLFEAHEILKYKPVYNRAQRRALYNY
                     GLYYFKNENGYIQFYLQKIEDECCAAPLTTFNSLQSAKNFLFDLCEKYQLCQKFCGLY
                     ETKGSCFQYKLHECRGACVGEESSSTYNLRVQGIIDRFSFSQSDFLLLGEGRDEDECS
                     VVKVENNRYVGFGFTAKEVNGYDTFDYYSDCVKNFPENKHVHSILKSYLNRNPNTVVL
                     KKQKSELME"
     gene            15889..17670
                     /gene="rpsA"
                     /locus_tag="PK345_04415"
                     /pseudo
     CDS             15889..17670
                     /gene="rpsA"
                     /locus_tag="PK345_04415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617002.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
BASE COUNT         5353 a         3278 c         3573 g         5735 t
ORIGIN      
        1 cggcgtaaat aaagttgcca cagtaatccg ttacgctgcc ggaggtggtt ttcatggtgg
       61 taacggtttt gcggagtttg gcgccagagg ctgttgcagg caattcacgg ggtgaatttt
      121 actatttctc cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
      181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn naccaccact
      241 atggcttata aactaatact aaaagtgagt tgttaagggg cgactatcat agcgccgcta
      301 tgcaaagttt taagattttc tccgaagagg tatgaagaag gagcatatgc tttattcata
      361 tatccaatcg caaatgcaga tgggcttgtt gcctcagatt caaaaccaat tgcaacagaa
      421 tgcattaatc ctgctttggc atctttacct aaggcaaaag tgttaatacc tgttgataaa
      481 gcgccttcac caaaagcaaa ggaatagttc ccggttgcct gcgtattaag tcctccagca
      541 aacgaccggg ttccagtaga ggtgttattg gttcctatag cagaagaata ggaaccgaaa
      601 tttactgcat ttgatctgca gctgacacat tgaaaatact cttcaacacc ttgggcgctt
      661 actgagtaaa tacctgccat taaagcaatt aataaaagaa ggtttttcat attcatattt
      721 attattagtt atagtatctg actatttctt aatgaatttg ccggtatgct ttggcatgct
      781 ctcgcctaaa atctcataca catacagacc ggcgggcata ttgctgatat tacagcgaat
      841 gatattacca ttaatagtaa gctttttgtc taagaacatg ataccattag tattgaatat
      901 tttgaagcga atattttggt ttggattcat tccttcgaga ctgacaaaca gttcagcatc
      961 tgcaggatta ggccacaatt ttaaattaag tttttccaaa gggatttttt ccactgttgt
     1021 agtgattacg atattttctc gcaaatactt tcgtatgtaa acatctcgtt cgagtacccc
     1081 ctgggcattg ggtttgttct tggcaatatg catcaggcaa ccgtcgtcgt gcatgggcaa
     1141 tataccagtg acttgatact gagcatcgcc gataaaaaaa cctgttgatc cgagtatgtt
     1201 catttcccgg tctattacga acaaataaac tgcattcggt gtcatgttgt ttgcaaaagc
     1261 ctgaaaaccg gctacatata cggtagagtc gttggcatag gcaatgcttt tgtgccatgc
     1321 cctataaata cttgtatctg gaatgctcaa caccaactct tcaaaaacct gcccggcagt
     1381 attcatccta tacacgccaa tgtaatcgtc aagataccgg ccatcgctga acgaattcgg
     1441 cccagccata agaaattctg attcgtttag ccaaaaatct gcgcttagca tgtcgtaaag
     1501 gtcggaaata taaaaatggt tgaccgcatg gatttgcata tttttatcca ttattgtaac
     1561 agtagggtgc cctgcagttg acattgtacc acctaacagc atcaggctgt cggtatctgg
     1621 tattatcatc aactcgtatg gcaactgcca gtaaggatat tgatagtaac ggctcattag
     1681 ggtatcccct tcctggttta atttaaacat tacataatct gtacggggac tttgtggatt
     1741 agtcacaaat tcaatagcct gtccaacgat gcagacattc ctgtcattgt caacaagcga
     1801 tttcatatat ccaccaaacc cggtataata ttcatctatt aaaaaaaagt tctcagcaat
     1861 tacttcaagc gcatgatcaa gtattaatac ccaaaaatag tccacatagt tccaatcatc
     1921 agttgctcca aaagtaccag taatcatata atttccatta tcaagaatat ttactgagca
     1981 taacctgttt agggtgtcct gtttattaaa ccgttttgtc atatatttgc catcaggttg
     2041 aaccttcatc accagtccat cacctttata ataatattca ccgattctcc ctacaagtat
     2101 tgcgtttcct tcaagatcaa ctgaaccatt aaaaatgtat ttgtcgtccg gtgttttata
     2161 aagaaaatca aagctatgtt gggcagcgcc atcaacagcc aaacaaatcg taaggagaac
     2221 aatcacataa attttcatgg ttttgagtat ttaattcagt attatcattg atccaatgtt
     2281 aatttctcta attattcggc ggttctaatt caacaggctc cccaatatcc tcaattggaa
     2341 ctatataccg gtaggcatat tcaagttcgt tttcgtgccg gatacattgg taatcaggat
     2401 tctgggcact tggacctgat tttcctatta atttacagaa aaaaagtacc caattatcct
     2461 gtttgttctc ttcctgtggg attctggtgt agatcacctc gcgttgtcca aaatagtgaa
     2521 agtttaactc ctcggaatcc aggcaataat catcgtatga gaagtttcca ttttcatctt
     2581 caatataaaa gatcaagtgg tttccgtttt catcttcgta ttcatgtcca aatttttcaa
     2641 ttatcgtgtc agtcacatat acatatctgt atccggttgg tggagggggg ggtatgggtt
     2701 ttgttgtatt tagaaaattc tgaatctctt ttgctgcatc ggttccagga ttactccaat
     2761 tacactttcc tttattctgt ccataccacc agttagcgct atcttcaaaa ggttcccatt
     2821 gtgcaggagg atttggatcg tgggcgcttt tctcaccagt aacagccaat aatacaagct
     2881 caatttgtcc atttacaatt tcacctgttt tgatagtaag caacaaaaag cctttatctt
     2941 caaaagcgct actgtgataa cactgggaaa caagaatgat tatgtcttca taaattgaaa
     3001 tagcatcatc aagtaatact tcaccactcg agttaacggg caggtaaatt atcgccgaat
     3061 cagcgattac cattccatat ttttcatacg gaaaaccgta agtaacattg aaaagagttt
     3121 caatgttcca cctcgcttct tcggcgctca tcacctcccc gctcttaaaa taggggttgt
     3181 cgcggtggta aactagcttt tcacggaaag cgatgatttt gttcattaca ttgatatctt
     3241 cggcagtcat catttcgtac tacgacttat taatggtttt gtcatggagt tcaactttac
     3301 tgcaccccac actgatcatg atcagcaatc ccatgatcgc agcaattgat ttgagaaatg
     3361 taaatttttt catagttttt agttttattt atgttattaa aaatttgtta tttacggttt
     3421 cttgtttaaa acaaagcaag actatgttta aggtggaaac atcattcggt aaaaagaggg
     3481 gttgaaaagt aaatgttttg tctcatagca cccttctatt ggtaacttga gtctttcgac
     3541 cggaattatt tttcgatggg ggctgtattg aaaacatagt tttttcattt ttagattcaa
     3601 tttttgtgtg taaaatattg gcgtcaaata taataaaaca tttcaaaacg tatgatattt
     3661 ttttgaaaaa tattcaaaaa tcttaggtga gttcaagaga gcctgatctg cgcatagtga
     3721 ctgttggttt aaaagtaaaa aaggttgttt gtagccaatt tcagcgatag tgctgaattc
     3781 tctccttaaa tcctaccaaa cacccactaa actttaaact gcggaggctg ttgcaggcaa
     3841 ttcacggggt gaattttact atttctccac caccactatg gcttataaac taatactaaa
     3901 agtgagttgt taaggggcga ctacttagtg aatctcttcg ggacaactat agagctacga
     3961 ctctataatt gtatttatgt attaacctaa aatcggtatt ggcttagaaa aggctcattt
     4021 gctcaggttt ttcacctgat gttgatttac ttttatgttg ttctgggatc ttggccgttt
     4081 caggcttttt taaaacctct ggctgtatag ccggtgtttc ttcaggtgta aattttactt
     4141 catttatatc attatcttca accggatcat tgtcgaaatc aggggttggg gcaccggttt
     4201 cattgtcttg atcttctaaa ggttcaagcc atttaacggt tttaataaag tgattagaga
     4261 ttctcttgcc tttcgcccta taacttttta ctgcgataaa ctcctctaca ttaatcgttt
     4321 gtgtatctat ttgtttttca tttcttttgt ggtcgaagaa tacttcgagg ttgggcctgc
     4381 tgtgttccga aattgtaaca agctttgctt cggggttgtc gcttacgaag tttatttttt
     4441 tgtcagtctc ttcaagggta aatcttttta gataatgaag gcctgttttc ggttcgatat
     4501 agatagcggt aaacaccttt tcgggatcat acttttcgat gtgaatcagg tcgggatcga
     4561 aatgtgtgct aaggtcgaac ccggtaagtc tgtattctcc tgattgcatc agagtagcta
     4621 ttttgtcgct tcctttaaat gcatttaagt atttaccgcg ttcctcggta ttcagtcgtt
     4681 ttacgctatc atcaaaccat atatccattg cgccaagggt agatatcccc tcctctttta
     4741 ttgtaacttt acgcacatag tttttggtaa ggatatttcc ttgtgtgttt cgccctttga
     4801 tatttaaatc gctaaaatca aagtcgaaag atgtttttct cagcttcggt ttaggtttta
     4861 gcagtacatt tactatttca gcttcgccgt ttggattagc cgataaatag agtactctgc
     4921 tatcaggggt gcctttagta aggttatact ctctatcgcg tgtaactcct gtaacagcaa
     4981 aacgtttcac gtaggtattg ccgttcttgc cgtcacgata tatagcgttg tagattgtcc
     5041 gcttatcatt tcgcttgaaa acatcaatat gaattatgct ttttccaaca aaaaatttgg
     5101 gtgaaacttt agttatcata tatgtcccgt cctctctgaa agctattatg tcgtcaatat
     5161 cggagcagtc gcagatgtat tcatcttttt tcaggccggt accggcaaat ccttcttcac
     5221 ggtttacata gagtttttca ttggtggccg caacaagttt ggcttcgata gtatcgaagt
     5281 ttcttatctc ggtacatcgc tccttgccgg gtccatactt tttctttatc gctttaaaat
     5341 aattgatggc ataatcaatc agatgtcgaa gatgattttc gacctcttca agctccaatt
     5401 ctatattttt aatgatttca tcagccttaa aagagttgta ctttgatatc cgttttatct
     5461 ttatttcggt tagttttatt atatcttctt tgttgatctc tcttttaaag agatttttat
     5521 atggcgtcag gcctttgtta attgcagtga taactgcttc ccacgtttcg cactcttcaa
     5581 tattgcgata gattcgtttt tcgataaaga ttttttctaa tgaagagaaa tgccattgtt
     5641 cttcaagttc acttttcctt atctcaagct ccattttaag gagattcacg gtgttgtcgg
     5701 ctgagatttt caatatctcg tccactccca taaagtgcgg ttttccgttg taaattacac
     5761 aagagttggg tgagatggca atttcgcaat tagtgaaggc ataaagagcg tcaatagttt
     5821 gatcgggcga tactcctgat tgtagttgta tctgtatttc aactttgtcg gcagtattat
     5881 cgtcaatttt tttaattttt attttccctt tatcgttagc tgcaattata gagtcaatca
     5941 ggcttgatgt agtggcgccg aaaggtattt cggtaatagt aaggtttttc ttatcttcaa
     6001 tttttattct tgctctaacc cttattctac ctccacgtat tccaaggtta tattttgaaa
     6061 aatccgctaa gccgccaccc ggaaagtcgg gtagtatttc gaactctttt ccctgtaagt
     6121 attttataga tgcgtcaata agttcgttaa agttgtgtgg caatatttta gaagccagtc
     6181 ctactgctat accctcaaca ccttgcgcca aaagtagcgg gaacttaacc ggcagcagta
     6241 tcggttcttt gttacgtccg tcgtaagagc ttgtccattg tgtggttttc ggattaaaag
     6301 ttacttcaag tgcaaatttc gacagtctgg cttcgatata tctcggtgca gcagcgccat
     6361 cgccagtaag aacattaccc cagttacctt gtgtatcaat aagaagatct ttttgcccta
     6421 actgtactaa tgcatctcca atggaggcgt cgccatgcgg atggtatttc atcgtttgcc
     6481 cgattatgtt ggccacttta ttataccggc catcgtccat ctcgtacatg gcatgaagga
     6541 tacggcgctg aacaggtttt aatccatcgg ttacatccgg cactgcgcgc tccaagataa
     6601 cgtacgaagc ataatcgaga aaccagtttt cgtacatacc atcgagcgca gtaattctgt
     6661 aattaccatc gctagccttg aattgaccag aatgatcgtt actatatata tttgccgaaa
     6721 ccggctcatg ctcatgatga tctttcagaa tttcgtccat tttacgattt agagattttt
     6781 gccttgcaat gttaaatgtt taaaatagga tttcgtttaa ctcctataaa caacgttaat
     6841 taccgatcag cgacaaaggt agctgaaatt ttcctaagat cagttgcaaa aaatttatag
     6901 gggttacttc accaacttgg aaattttttt aaacacgtcg gttcccgaga atcttaatag
     6961 ttcaaataaa atagattcat aggtagagat gatagctccc tcttgtcgca ttctttctat
     7021 tgcaagggct ttgtcattta aacttcttga tccgacgcaa tcttcgatta ccactggctg
     7081 ataaccttgt tttagcaggt caatagtagt ttgcaataca caaacatgag tttcgatccc
     7141 tgcaatgatg acatttttta tctggtgttt gctgaattct ttactaaaaa gctgatcatc
     7201 gcaacagcta aaggcggttt tttcaattac cggtatatcg cttaccaatt gtttcagagg
     7261 ttcaatggta aaacccaagc cctttggata ttgttcagtt accataatat tcacaccgat
     7321 agctttcaat ccttcgataa ggatagctgt attgcgtgtt atctgttcat tatctttgat
     7381 gaatggatag agcttttcct gtatgtctat aataagtcct aaagactcgt tggctaaaat
     7441 tcgcatgatg ttatgtttta ttttaaaaat ctcaatagaa gtatctctcc aagtaaaaag
     7501 gcaagagcaa gactaataaa aagttttgtg agctttattc cggcacttag ttcatcaata
     7561 acttctgtga gcgatttgtc ggttggcttt attacctgaa aatgagtcag tcctctgtcg
     7621 gtaaccatct tcgcaaggtc ggcacgtgaa taaaaatcca gatccgactc ttttctgtta
     7681 tagttaaagg ctaatcctgc aagtgtttcg tcattataca ccactttata gtgccctgct
     7741 tcctttatct gattctgggt aaatatttct gtttgcgaaa agagcgagcg tatttcgggg
     7801 attacttcaa aatctttgtt tgtacttctg attttaaaga tctgatcacc cacactttgg
     7861 cttttattta tggtgattct ctcattttct cccgctgtat ggtatatcac agatgctcct
     7921 tggctaagca ggccaatttt gtagagcgtt ggcacaaaca tggcgtgttt aacaaagttt
     7981 gaccattcat ggtttaaagg caccgcaaag aggtaaagca tcccatttac tgctggctcg
     8041 ctacccataa agatatcacc gttcagcagg tcgagttggt tctccatcat cgagccggta
     8101 ggccgccgaa tcagatagta cgaagttact ttaggcaggt caatattttc ctgaaccgac
     8161 tcgaatacat catcataaat gctcgaaagg gtatttatac cagccactct tgtatcgctc
     8221 gttattttat ctgctaaaaa agatgtattc agacttataa aaagctgacg ataactgtca
     8281 aagtctgctt cctcgccagg aaatatagcg atactgccac ccgtttctgc aaacttgttc
     8341 agctcgatag tcaaacccga agatattttg ttaagtccat tgaggatgat aaggttatat
     8401 cttgagaagg aggaatagtc aagcctgttt tctgcttcat tttgaaagaa aaaagaggaa
     8461 tcgttttgaa ataatgagtt tagataaagg ctctcttcgt cagcgtttat ggcaagtatc
     8521 ggaatcagct ccttgacgtc gaaagtaaag tagagaaggt cgtcataggt gattggaaaa
     8581 tcggttattt cgagtgttcc gtattgtatc cccgattcat aaattgtaaa gggtagctcc
     8641 agttcgatct ctttccctgc attaaggtcg aagctggtaa tagctctttg ctggccatta
     8701 atggtaagtt taaccggaac tttttcaaaa tcctgatctg aggcgctttt gatctttaca
     8761 ttcagtttta tcagttgccc taacctaaag gcaggcgcat cgaaccaggc gctatcaata
     8821 taaagattac tcttttctat ggcttctacc ggaatcaaga atccattaat tgtggtatcg
     8881 ggttttatct gatcaacatc ggttatattt ttctgaaagt cagaaatcag acaagctatc
     8941 cggctttcgt atctgttttc atgaaacagg tctgcttgac gatgcatgac ttcagaaatc
     9001 attttcctgg cgggcgaaat ttccacttca tctaacaatt gaaggaaatc ctgacgatta
     9061 acaaatcgct gatgaacgcc ttgaaaatca tttgtcagaa gctgaaatac atcagttgtg
     9121 ccgtaagcca tcgccacctc gcgtgcctta cccaatgctt cgtcgagaag cgccccttgc
     9181 gaggaggtaa gtcccatgct aaatgaatta tcaatgtata tgctcaccgc attgcgagct
     9241 tgctgtttca tggaagcatc agaggtgggg atgtaaggct gtgcaaaagc aagcgctaat
     9301 gacgcaatgg caagaatacg cataagcaac gcgagcagat gcctgatctg cgactgcttc
     9361 tgagtcttct gttgaagctc ctccaaaaac cttacattag taaaatagac cttcctaaac
     9421 ctcctgaaat taaacagatg gataacaata ggaatagcta ttgcagcaag accatataaa
     9481 aacaaagggt taacaaattc cataggcaaa atccaacatt gatttattcc tgataaaacg
     9541 caggtaattg aaattattat gctaacgaca ggcaattttg tatggaggta gaggtaactt
     9601 tatattaata taactctcaa ttattgcagt gattgatact agtgtttgat gaaaaaaaag
     9661 atctgttaat caattgcaat tcagtattca gtatgtagaa taattcttac aaattttaat
     9721 cctgtatctg agagttatgt aaaaagcatt ttttgtttcc tcaatagaat tcatcttctg
     9781 ttttttcttg ataatcatct ttggattttt ctattcagta aagacttttt attattatta
     9841 aattgttttt attgatatgg attacaaaga aatttcggaa atggttgtta aaaatgagtt
     9901 ttaggcttgg ttttgcattt aggctgaatc attaagtgga gatagggctg tctttttatt
     9961 atgcacacaa gcctgctata tgtctttgcg aaaaatttga taaaaaaaaa gatgtcaaac
    10021 tattaaagca tggttaaaat ttacaataat tcggtgtaag caaatcaaat tagtttgttg
    10081 gtgaaatact ataggctttg ttttcttaaa atggctaatt ttgccgccta aaaatatttt
    10141 tgtttattta aaattttaat ttttatggga aaaggcgatc gtaaaacaaa gagaggtaag
    10201 attgtacttg ggacatacgg taagagaagg ccgagaaaaa cttcaaaggc tgctgtagtt
    10261 ccagtggtag aggctgaaga gaaaccaaaa gccaaaacgg ccaaagcagc caaagcaaca
    10321 aagaaataga cttatcttac ataagaatca ttgaggaaac tcacgggttg tccttgtcgg
    10381 gaagctcgtg tttttctttt atactattta tcatcgcttt aaaagcccta ccccgttctt
    10441 caaaattgtt aaactgatcg taactggcag cagccggcga gagaaggcag attttatcct
    10501 tttgggtgta atcaacaatt attttttccg tttcactcat acgattgata aagaacaggt
    10561 tttttgtagg tctattcttt ttagcaaaca gcctcatcat cctttctccg gcgccgccca
    10621 taaagatcaa attccggata gccgaatcag ctaataggtt tactaattta ttatagttaa
    10681 cccctctgtc atgaccgccc agaatgatgg tgtcaacgtc aagtaaagtg tgaatagctt
    10741 caattgtgga ttcgggtatg gtagagatgg aatcgttgat aaatcgaata tctgctacaa
    10801 cacctaaatc ttctaaccta tgttccaatg gtttgaatgt aggaagccct ctttcgatat
    10861 ctttcgcttg tagtccggct ttcgataaaa ttgatgctgt tataataatg tttttaagat
    10921 tgtggcgacc atgcagcata ctgtttttaa cgcagattgg ctcttgtaat cctgcaatat
    10981 gtatcgtttg gttttgtgca tcataaattt tgtctgatag ttgaatggct atcttttgtc
    11041 cattgtactt gtaatctgat agcagttttt ttataatcaa attcgaattg tcaaatatta
    11101 aaaagtcgtt tgtttgctga tattttgcaa tattgaattt tgctgaagag taatcgtcca
    11161 tcgaggagaa ataatctaaa tgctcctgaa aaatattgag caaaacggca atatgtgggg
    11221 agtggttaat atactcaagc tggtgtgctg agatttcgaa aattattgtg cttttccgac
    11281 taatattttg gagtagatcg aaaggtggaa taccgatatt tccaaccaaa actacttctt
    11341 tgcctgcttc tttgagtata tgataggtga ggcttgctgt ggtgctcttc ccttttgtac
    11401 cggtaattcc aattgtttgg gtggaatatt tttcaagaaa aagggaggtt tgtgagacga
    11461 ttttttgttt gattgaacta tcaatgcttt ttccaagatt aataccggga gatttgaaaa
    11521 ctatatctgc ccagcctaat gcatttaaat aatccttgcc aagaaagagt tctgatactt
    11581 tttcattttc aacttcggtt agcggcaagc ccggatttct atcggcgata gccatcttaa
    11641 gagaggggaa aaaatattgc aacaaccgga atgttgacct gccctctcgg ccaaatccaa
    11701 ggataagaac ctttttatct tttatgtatc tcctcaatag ctctttcatc cgattgcttc
    11761 cttcaatatt ttcatgaatt gaatgggtac gaaattatct cgatttaact ctttcattat
    11821 aatttcaaat gaggggtcat tctgttttcc tgcttcaacc gactgatgat aatgtttaag
    11881 aagagcgggt agtttagtgt tgtatctttt tttaattgtc atcgccaaag ccatatttgt
    11941 ttcatcggtg gttccgatac actcaaaagg cttttcggaa gcctgcccgg taagttgatt
    12001 taaaacagga agtaaagtaa tgtcgtcgaa taagttagtt ccaaatattt tgacgagttt
    12061 ttcttcttcc atgaaaggcg agagaattat ccatgtaaag aggcattttg ggcatttgca
    12121 gcaccacgaa tcggttttac ttgctacgtt gcaactttta aaatgaggat gctgatctgg
    12181 aatagctgcg aaaagcttgc ctatctgcag ttcattcaat ggtcttagaa aactgaaata
    12241 ttcaatgttt tgggttagat attgatttgc atatcttctg aaatcatttt caaaatctat
    12301 tgatttggaa tactgatggt tgatttgagt tcctggaatg gtgggctcgt ttgcactgga
    12361 ttcgtttgaa agtgcaatgt aacggcttcc tgaaagtgag gctgccagca ggctgataaa
    12421 ggctaataat gccgaaaaag gagtatgccc gtttaaaaag ccttgtttgt taagctctaa
    12481 aagcttaggg tcgagagtgc ggaagatggt aaaaatctgt ttctggtcaa aacctgacgt
    12541 tatggcagtg gctaaagatg cggctcgtgg attaagaata aaaggaataa tttcgaaacc
    12601 tgactttttt aaaagttcga gtgttaccgc cgaatctttt ccgccaccta tcggaataag
    12661 aatcctatta ccaacttcaa cctcctccat ttcatactct tttgaactgt tgatttcgat
    12721 attcaaaaat tcgtgaatat tagtttcgat accatttaca tagaaaaatt cgcccaagcc
    12781 tttaaagtaa acatttttcc accattcgat ctgaaaatca catagatatc cggcgtcaac
    12841 aacaaccttt tgaggacatg ctgctttcca ataactaatc agctctatca tgccgatttg
    12901 aaaagaaagg ttttccaaaa aagagatgtt taaaattttg cttgagcttg atttcagagg
    12961 aatgtttaat tgtggataga aattgtattt atccgacaaa ttgaaatggt acttaaccct
    13021 gatatgatcg tttttagttt caatgctgaa gcctctataa tataagtgag tgaaattttt
    13081 tcttaaatta tcaaaatggc tttgattttg ctgaaccagc attgcaaatt atttatatat
    13141 ttgtttcgac aagttaaata accaaaatta aagaaattaa ttttgcaaga tggaaaactt
    13201 tgatgacctg ataaagataa agactaacaa cacacgtccg gcacgcggaa gaatacttat
    13261 ctccgagcct tttctctatg attattattt tcataggtcg gtcattttgc ttgccgagca
    13321 taacaaagag ggctcttttg gcgtcataat caataagccg cttgcaatgg gatttggtga
    13381 agtggtaaag gattttcctg attttaatgc tactgtttat cttggcgggc cggttagcgc
    13441 tgatagtctg ttttttattc acaccttagg ggttgagatt gatggtagta tggagattat
    13501 ggaaggattg tactggggtg gagaccttga gcagataaaa tcctatatca ctattggtcg
    13561 ttttaataat gataatattc gtttttttat tggttattca ggttggtctc caaatcaatt
    13621 agatagcgag ctgcaaagaa attcatgggc tgtttcagct cttgatgccg atcagataat
    13681 gaaagccgac cctgctggta tatggaagtc gagccttatt gacttgggag ctgaatactc
    13741 taaatggaca aactaccctt ctgatccggg gctgaactaa ctggtcagat gaaatatgct
    13801 attttagata tcgaaacaac cggaataagc tacaaaaatg gtaaaatcac tgaaatagct
    13861 attctcatcc atgatggtaa agcaatcgtt gatgagtttg caacgctcat taatcccgaa
    13921 caaaaaattc catattacat cacacaacta accggtatca acaacaggat ggttgaagag
    13981 gctccaaagt tttatgaggt agctgccaaa atcgttgaaa ttacccagga ttgtactata
    14041 gtggcacaca atgcaagttt cgattataat tttctacgtc atgaatttaa ggcattaggt
    14101 tacgatttta atcgccagaa actatgcact gttaaactaa gtcgaaagct aatacctcat
    14161 caacgatcat atagccttag caatctttgc cgtgagttaa atatcccaaa tctgcatccg
    14221 caccgtgcac ttggtgatgc caaagcaact gccgtgcttt ttgatatact gttgaaaata
    14281 gaccctaatc taagccaact gccgtttagc ggaaataata gcagccttaa acctgaaatg
    14341 attaatgctt tacctgaaaa aacaggtgtg tactatttta ttgatgctga tcaaaatatt
    14401 gtttatatcg gaaaaagtaa aaatattaga gaaagggtaa ttgcacacct aaccaattcc
    14461 atgacccaac gggcattgga gatgaaaaat aaattagccg cagtggactg ggagcttacc
    14521 ggtaatgagc tgatagcgct gctttttgaa gctcatgaaa tacttaaata taaacctgtt
    14581 tacaatcgtg cacaaaggcg ggcattatat aactatggcc tttattattt taagaatgaa
    14641 aatggttata tccagtttta tttacaaaaa attgaagatg aatgttgtgc agcgcctttg
    14701 actacattta attcgttgca aagcgctaaa aattttcttt ttgatttgtg cgagaagtat
    14761 cagctatgcc agaagttttg cggattatat gaaaccaaag ggtcttgttt ccaatacaag
    14821 ttacacgaat gccgtggcgc atgtgtaggg gaggagagtt caagtaccta taaccttcgt
    14881 gtgcaaggta ttatagatcg ttttagcttt tctcaatctg attttctgct cttgggtgag
    14941 gggagagacg aagacgaatg ctcagtagtg aaagttgaaa acaaccgtta tgtaggtttt
    15001 ggttttactg cgaaagaagt caatggctat gatacattcg attattattc cgattgcgta
    15061 aaaaacttcc ctgaaaataa gcatgtccat tcaattttaa aaagctacct caaccgaaat
    15121 ccaaatactg ttgttttgaa aaaacaaaag agcgaattaa tggaataata gtaaccacgg
    15181 agtattgtgt tttaatccta attgttcaag agaaaacctt ctttttttga acaattgatt
    15241 ttattgaaga aaatattagt acgcttgtag ttaaatatct ttcccattaa caaaaaaaag
    15301 ctggttgctt aaaaaataat tctaagattt gaataactta taatcttaaa tttcaagtag
    15361 atagtacgaa taatagagat tttaaagtaa ataaatgata ttcgataatt cgtgtgattg
    15421 aaatttatct acttttgcac cccaattgca aatgatgctt ttaacgccta aaaatcattc
    15481 atttgcatgg tttaaaataa ttattaatct ggataataag gcggtatcct taataaattt
    15541 ttaatgacta acgaacaaga aacaaacaac caggagcctc aagatcagtc aactccgatt
    15601 gatgaaagct tacaggttga acaacctaag gcaactaaag ccaatgaagt agagacttct
    15661 gaagaggttg gtaaggaaga agctgaaatg ccgaaagatg agagtgctgt agaaactcca
    15721 gaagaagttg atataccgaa agatgagagt gctgtagaaa ccccggaaga agttgattct
    15781 ccaaaagacg agattgccct ggaaccccca acagaagttg aaaaagctga gactacaact
    15841 gaagaacccg ttgtggaatc cgaaaaaacc agtgatgttg aaaaaaacat ctatcgcaca
    15901 ccattaaagc cagatgaaaa ttttgactgg agtgccaaag gaaaaattga acagacttac
    15961 accggcgaag agcgccagaa gcttgaagag ctttatagca aaacctttag tactattgcc
    16021 gaacatgaag tggttgaagg cacgatttcc ggaattaccg atcgagagat tgtggttaat
    16081 atcggtttta aatccgatgg tgttattcca aaaggcgaac tccgctatag tccaaatatt
    16141 cgggttggtg atcccattga agtatatgtt gaaagtcagg aagatcaaac aggtcagctg
    16201 ttgttgtcgc ataaaaaggc aaggctattg aaatcgtggg aacgtgtaaa tcaggcattt
    16261 gaagccgaag agatcatcaa tggctatgtg aaaagcagga ctaagggcgg gttgattgtg
    16321 gatattttcg gtattgaggc ttttctccca ggttctcaaa ttgacgtgaa gcccatacgc
    16381 gattataata tttatgttga taaaatgatg gaattcaaga ttgtaaagat caatcatgaa
    16441 tttaaaaacg ttgttgtttc tcataaagcg cttatcgaac gtgagcttga gcagcagaaa
    16501 gccgaaatta tttcgaagct cgaaaaaggc caggtgctcg aaggtactgt taaaaatatc
    16561 acaacttatg gtgtgtttat tgacctcggc ggtgttgatg ggcttattca catcaccgat
    16621 ctgtcgtggg gacgtattaa tcatcctgaa gagatagttc agcttgacga aaagataaag
    16681 gttgtaatcc ttgatttcga cgacagcaag aaacgtattg ctcttggcct taaacagctt
    16741 actcctcatc cgtgggatgc gcttgaagag ggaataaaag ttggtgataa agttagcggc
    16801 aaagttgttg taatagccga ttatggcgct tttgtagaga taaatcctgg agtagagggg
    16861 ctgttgcatg tatccgagat gtcgtggtcg cagcatttac gcactgctca agatttcttg
    16921 aaagtgggtg attccgtaga agccgtaatt cttacactcg atcgtgaaga acgaaaaatg
    16981 tcgttgggga tgaaacaatt aattcctgat ccatgggaga acattaaatc tcgttatcaa
    17041 gtcggttcca aacacactgc tacggtacgt aattttacta cattcggaat ttttgtcgag
    17101 cttgaagaag gcgttgacgg tttgatccat atttcagacc tctcttggtc gaaaaagata
    17161 aaacaccctg ccgaatttac caaagtaggt gaagagatag aagtagttgt tcttgatgta
    17221 gatgaagaaa atcgccgttt gagcttagga cataagcaat tagaagaaaa cccgtgggat
    17281 gttttcgaaa ctgtatttac tgttggctca atccataaag gaacgatcgg acagcctacc
    17341 gataagggta tgttagttat attaccatac ggtgtagaag ggtttgctcc aacacgacat
    17401 ctgcaaaaag agaatggtaa tattgctact actgatgaat cgcttgattt taaagtgatt
    17461 gagttttcga aagagaataa aaaaataata ctttctcact caagagtcta tcaggatttg
    17521 caacaggtag aaaaagagaa agaagagaaa gagcaacaaa aagctgaacg ctcaactaaa
    17581 aaggctgtta aaaagataaa agagacgctt gaagtaacta cctttggtga tatcgacggc
    17641 cttgctgagc taaagtcgga aatggaacag aaagctcgcg gtaaactcga agagtatgct
    17701 aagaaacaga aagaaaaaaa ggagaagaaa gagaagaagg agaagaaaga gccaaagtct
    17761 aaaaaagatg atgaacaact tgctttgaat aaagatgtag ctgagcaagt tgaaaaagct
    17821 gatgcggcca ttgaagagat tgctgataaa gctaagttgc cagaagcgcc tcaaaccgaa
    17881 gaggttgctg aagctaaagc agatgctgcc cctgaagagg ttgttgaaaa ggttgtcgaa
    17941 gaggtcgttg aagaggcaac agaggaggtt attgaaccta tagcctcaca atccgatgag
    18001 attgctgatg caaaggaaga caaagatgct gacgaagaa
//
LOCUS       DAOOAI010000042        17955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_4126_length_17955_cov_4.964469, whole genome shotgun sequence.
ACCESSION   DAOOAI010000042 DAOOAI010000000
VERSION     DAOOAI010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17955
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4126_length_17955_cov_4.964469"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..263
                     /locus_tag="PK345_04420"
     CDS             <1..263
                     /locus_tag="PK345_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FliG C-terminal domain-containing protein"
                     /protein_id="HPX34297.1"
                     /translation="ESAELEEIKNLSNAKVKALLKEIDVQSIWYALKDASDEVTDKIL
                     PNLSKKAKVEYDLLSEKIKKISKADIIEARKKLKDRISNLLS"
     gene            656..4900
                     /gene="glgP"
                     /locus_tag="PK345_04425"
     CDS             656..4900
                     /gene="glgP"
                     /locus_tag="PK345_04425"
                     /inference="COORDINATES: protein motif:HMM:TIGR02094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HPX34298.1"
                     /translation="MTFDKLLKPNYLFEISWEVCNMIGGIYTVISTKAPAIKEKLGDQ
                     YITIGPDVWKETRKNPTFTEDKSLFAEWRDSAAANGIYVRVGRWNIESSPIAILVDFT
                     NLFPEKNNIFAKLWERYQLDSINGQWDYIEPAMFGHAVGKVIESFYNYYLSYSYNVVA
                     HFHEWMTGTGVLYLNEFLPQVATAFTTHATTIGRSISSNGLPLYGKINEYNGDEMAKK
                     LGIVARYSLEKKSVATADAFSTVSYITANEAEKLLGKKVDVVTPNAFSTSLFQNEETY
                     SKKRNIARAKMIQVLEALFNEKIDTDAFLTITSGRYEFRNKGINIFIDAIEKLSTANP
                     DKQVVAILKIPANHSGPRNDLLQRIASPDFGNPLSGEYLTHGLHNASDDAILNKIART
                     GLKNTPGSKVKIVFVPAYLNGDDGIFDIPYYDLLPGFDLTVFPSYYEPWGYTPLESIA
                     HRVPTVTTNQAGFGRWVLDNFPENHSAVAVLNRPDDDINTLDAEISDIILKTISLSDD
                     ELTALRKNAYEIATKANWKEFVNYYYKLYDIALEKVDERVETFPALQIVKRKVKLTEK
                     TVIDTPVWKKIVIEPKIPEKLKGLQKISRNLWWSWNFKATDMFENIHPEKWEEAGQNP
                     ITLLDSLNIDEWKALENDKDFYEKYLESYNKFEAYLSESVDKSIDAVAYFSMEYGLHH
                     TLKLYSGGLGVLAGDYLKEASDSNKNLVAIGILYKYGYFQQSISTFGDQIAEYQPQKF
                     SQLPIERVYGDDGKQLHVEIALPGRTLQIRIWRCDVGRIPLYLLDTDFNENVEADRRI
                     THQLYGGDLENRLKQELVLGIGGIRALEVLQIHPAIYHSNEGHSAFIGLERLRYLIQT
                     DKLTYHQALEIVRSSTLFTTHTPVPAGHDVFDEDLLRTYLPHYPERLGLTWDEFMNLG
                     KFAENDINNKFSMSALAAKLSQEINGVSRIHGRVSQEMFQKLYDGYYPSEIHVGYVTN
                     GVHLPTWASKPWKQLYKKIFEEDYKQKQLNFDMWQKVHSVEDSIVWESRKQLKSELIV
                     YLKQRLRDEMTKRQENPKLILQTIDSLNPNALTVGFARRFATYKRAKLLFSNIERLEK
                     LVTISERPIQFIYAGKAHPNDKEGQDLIKKIIEISKTKPFIGKIIFVENYDMNLAHKL
                     ISGVDIWLNTPTRPQEASGTSGEKAVMNGVVNFSVLDGWWAEGYRENAGFAIQEAKTY
                     ANQQFQDELDAEIIYNLFEDTIMPLFYDLNNDKIPIAWIKYIKNTVSDIAPHFTMKRM
                     IEDYYSKYYYKLIERTKRIEKNNYQLAIDYAEWKRTIRSKWGELKLEKLMVSDSDSPI
                     YLEDNFSAEVTLFTNGIPAQNIGVEIIFSIKNNKGARKILLTREMSLLEVINGKALYS
                     VDVPVVNAGTFNYGFRVFPKHELMPHRQDFPLVKWI"
     gene            4935..5393
                     /locus_tag="PK345_04430"
     CDS             4935..5393
                     /locus_tag="PK345_04430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5606 domain-containing protein"
                     /protein_id="HPX34299.1"
                     /translation="MTDLNKIYTISGKPGLHKNVAVSKTGLIVESLIDGKRFNVFAHE
                     KMSALGEISIFKVGGDILLIEVLKKIKEKYESKPVANADFSGPQLRVFMEEILPDYDP
                     ERVYTSDIKKILSWYNLLAEKDMLEFKEDEPEEEHASEQNTEPNTDESAE"
     gene            5413..6453
                     /locus_tag="PK345_04435"
     CDS             5413..6453
                     /locus_tag="PK345_04435"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HPX34300.1"
                     /translation="MYKLLIRPLLFLFQPETIHSFVVKGMKFWFKIPGIACFIRQIAS
                     VNSTKTEKEVFGLKFKNPIGIAAGFDKQAEFFDELANFGFGFIEIGTVTPKPQPGNPL
                     PRIFRLPNDKALINRMGFNNIGVDEAVKNLKKRKTNIIIGGNIGKNTLTPNENAVDDY
                     IYCFDKLFDYVDYFVVNVSCPNVSDLRELQDQKSLMEILGKLQSINNLKERPKPILLK
                     VSPDLNWQQLDEVIEIVHNTGICGVVATNTTITRDNLTASTKRVQEIGDGGLSGLPLK
                     NRSTEIIRYLSEKSGKAFPIIGVGGIFTPDDALEKIGAGADLVQVYTGFIYEGPFIAK
                     KIVRKIISNQMK"
     gene            complement(6506..7213)
                     /locus_tag="PK345_04440"
     CDS             complement(6506..7213)
                     /locus_tag="PK345_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005864241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPX34301.1"
                     /translation="MKPKLFLIPVTLSDTNPKEVLPESVFKIIYSLDEFIVENIRSAR
                     RFLRKTGYTKDFDHEVTFYEIGKHHNSDEIIELLQIDTRTKSLGLLSETGVPCVADPG
                     AGIVKIFHQHGFQIAPLVGPSSIILALMASGFNGQNFAFHGYLPIDRNDFKQKLKQLE
                     KTIIQNNQTQIFIETPFRNNKLLDAIISNCSINLKLCIASNLTTSQEFVISRSISEWR
                     ENLPDLNKKPTVFLLYK"
     gene            complement(7217..8179)
                     /locus_tag="PK345_04445"
     CDS             complement(7217..8179)
                     /locus_tag="PK345_04445"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HPX34302.1"
                     /translation="MHSKTIPTATDILTAASRIRPYIHRTPVLTSSSISKLFDCQLFF
                     KCENMQKAGVFKSRGACNAVFALDDNSIIKGVATHSSGNHAAALSRAAKLRGTKAYIV
                     MPNNSSKVKIAAVERYGGIITFCQPNLASRESTLASLIEDTDAVEIHPYNEPLVIEGQ
                     ATATLELLEDYPDLDIILAPVGGGGLLSGTALATSYFCRKTIVIAAEPEQADDAYRSF
                     HIKSLVPSINPDTIADGLRTSLGSITYPIILKYVNDIVTTSEDAIISAMKLVWERMKI
                     VIEPSAAVPIAALMEDKIRCRNKRIGVILSGGNVDLSNLPWQQA"
     gene            complement(8190..8669)
                     /locus_tag="PK345_04450"
     CDS             complement(8190..8669)
                     /locus_tag="PK345_04450"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HPX34303.1"
                     /translation="MGSKPKTKILMVCLGNICRSPLAEGIMQQKINARSLNAETYSSG
                     TSNYHISEPADPRTIAIARHHGVDITTHRVTQFKADDFDKYDMIFVMDNSNMKSVLRL
                     ARTESDYKKVRLIMSVISDRKSMEVPDPYYSDKEGFELVYNMLDEACEKIADELENL"
     gene            complement(8687..10120)
                     /gene="dnaA"
                     /locus_tag="PK345_04455"
     CDS             complement(8687..10120)
                     /gene="dnaA"
                     /locus_tag="PK345_04455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HPX34304.1"
                     /translation="MEKSAQKVWDNCLKIIRDNINHQSYETWFQPIRPLRLVDNVLTI
                     QVPSQFFYEWLEEHYIDLLKKTIKKELGTSGRLEYSVLIDNTGNNTDPFSIIMPTSNR
                     SATKNPGVSLPLTLKGTTKIIPDPFVIPGLQKVKINSQLIERYSFDNFIEGDCNRLAR
                     SAGLAIAKNPGKTSFNPLFIYSKVGLGKSHLAHAIGLEVKNNFPEKTVLYVNTDQFMA
                     QYIESVRNNNVNDFTNFYQIIDVLIIDDIHFLTGKEKTQDIFFHIFNQLHQKSKQLII
                     TSDRPPVEIEGIESRLLSRFKWGLTADLQTPDLETRVAILQKRLYNDGITMPDNVIEY
                     LAYSITSNIRELEGALISLLAQASLNKKEINIDLARQIVDNFIRSTNKEISIEYIQKV
                     VSQYFDLSVEEMNSKTRRRNIVQARQLAMYFAKEHTKASLTTIGLHCGNKDHATVLHA
                     CRTVRNLVETDKKFKSYFNELERIIKH"
     gene            complement(10723..11997)
                     /locus_tag="PK345_04460"
     CDS             complement(10723..11997)
                     /locus_tag="PK345_04460"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPX34305.1"
                     /translation="MRVRLSIISVISVLLFTGQLSAQLPQLPDSTFACRADSVLLDAG
                     PGFLSYLWNTDEQTQTIYAKTPGWYFVWCTTEDLIIIQDSTFVMFFNAWIEQPDTIQT
                     CYLYPVILSINTDTLQYQWSASDPDLVFSKPDRSAVEFVPNLDTTTVYVHITDSLNVL
                     TCMDSVQIFLYPRIYFDEVNQINTGCPGTCKGQLQVLVSGGLPPYSYYWPNASPVQSD
                     SIAFGLCEALYRIEVTDEYGCVRDSLVPVKVFDMPIVNIIRNPEEQIYIQNPVVQFSF
                     ENTSIDSIQIIDWNWNFGDTTFSTKENPEKVFDQVREYEVKLKYTTSNECVDSVMLKV
                     DVGQAEISIPNVFTPNGDGYNEIFTIKDLEYYMSNELMIFNRYGKRVYSTNNYQGNWD
                     GDNLKDGVYFYVLKAKGYFGTDIFKGSISIMR"
     gene            complement(12072..12443)
                     /locus_tag="PK345_04465"
     CDS             complement(12072..12443)
                     /locus_tag="PK345_04465"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HPX34306.1"
                     /translation="MAELKKTLVLGASPNPSRISFKAVNNLVRAGHPVVAVGQRVGNI
                     GNIAIAIDNPSVSEIDTVTLYLAPENQPDMYDYILNTISPKRIIFNPGTENAELSSLA
                     KQKGIETQEECTLLMLLKGIY"
     gene            12704..15298
                     /gene="clpB"
                     /locus_tag="PK345_04470"
     CDS             12704..15298
                     /gene="clpB"
                     /locus_tag="PK345_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HPX34307.1"
                     /translation="MNLNNFTLKAQESIQKAQEIAVGSQHPQIENAHILKGLLISDDN
                     VVPFLIKKLDVNLTHLQSELDKLLNGYVKVSGGQIYLSPNATTALQKSLTFLKDFNDE
                     FVTVEHILLGILSVKDDVSDLLTQHGVTLQKLIRAIKELRKGSGVKSQTAESSYNALN
                     RFAKNLNELAAAGKLDPVIGRDEEIRRILQILSRRTKNNPVLIGEPGVGKTAIAEGIA
                     HRIVNGDVPENLKSKHIYSLDMGALIAGAKYKGEFEERLKSVIKEVIESDGEIILFID
                     EIHTLVGAGASEGAMDAANILKPALARGELRAVGATTLKEYQKYFEKDKALERRFQIV
                     MVNEPDTMDAISILRGLKERYETHHQVRIKDEAIIAAVELSQRYISDRFLPDKAIDLI
                     DEAASKLRLEINSLPEELDEIERKIKHLEIEREAIKREKDKEKLAVLSQEIADVTEKR
                     NHLRTKWMAEKEVVDGIQKKKREIEDLKLNAEQAERDGDFGKVAEIRYGRIRHADEEL
                     EKLKAKLTSIQGDSPLINEEVDSECIADIVARWTGIPVSRMLQSEREKLLQLEEHLHE
                     RVVGQDEAIEAVADAIRRNRAGLQDARRPIGSFIFLGTTGVGKTELAKALAEFLFNDE
                     NSMVRLDMSEYQERHTVSRLIGAPPGYVGYDESGQLTEAVRRKPYSVVLFDEIEKAHP
                     DVFNILLQVLDDGRLTDNKGRTVDFKNSIIIMTSNIGSHQIQEGLGKGINTGDDSNFE
                     KVRLEVFELLRRTIRPEFLNRVDEIIMFRPLSEDQINEIAQLQVNILKEKLEKVGIDI
                     TVTPQAVGWIAHAGFDPQYGARPVKRVIQRQLMNELSKMILAGNVSKDESIVIDADKN
                     GLTFAKEN"
     gene            16233..>17955
                     /locus_tag="PK345_04475"
     CDS             16233..>17955
                     /locus_tag="PK345_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HPX34308.1"
                     /translation="MLDRIIGFSLKYKLIVILFTLTIVGFGIYAIFNIPVGAVPDITN
                     NQVQVITTSNNLSTQEIEQFITAPVELEMANLPGVQEIRSVSKFGLSVVTIVFKESMG
                     TYLPRVLIAEKIKTASADIPEGFGTPEMGPITTGLGEIYQYVLDVKPGFEDRYTAMDL
                     RTIQDWIVRRQLSGIDGVVEVNSWGGYLKEYEVAVNPARLQAIDVTLMNVFDALEKNN
                     SISGGAYIEKKSESYFIRGDGQVKSIEDIENILVKNKGGIPVLVKDVAKVHFGYANRF
                     GSITANGEGEKVLGQIMMLKDANSKKVINEVKARVAEVQKTLPEGVYINPILERSELI
                     SKTTTTVLENLIFGCIIVFFVVLMLLGNMRSALVIASMIPLSLLFTLSMMFIFGIDAN
                     LMSLGALDFGIIIDGAVIIVEYVVVRLTANQLQFRNTSPEERLKLNDEITKESASTMM
                     KSAIFGQFIILIVFIPILSLQGVEGKMFRPMALAFGFAIIGAMIFCLTWLPVISSIFL
                     RPVDVSKKNFSDKIIEFAQKSYQPVLKWALNKKALVFTLSFLTLILSGLIFNRMGGEF
                     IPTLDEGD"
BASE COUNT         5737 a         3215 c         3535 g         5468 t
ORIGIN      
        1 aagagtcagc agaattagag gagattaaaa atctatcaaa tgcaaaagta aaagcgttac
       61 taaaggaaat tgatgttcaa agtatatggt atgctctgaa ggatgcttcg gatgaggtta
      121 ccgacaaaat actaccaaac ctttcaaaaa aagcgaaagt cgaatatgat ttgctctctg
      181 aaaaaataaa gaaaatcagt aaagctgata tcattgaagc tcgcaaaaaa ctaaaggatc
      241 gaatatctaa cctgctatca taaacagttt gaaagattaa aacatctttt gagttaagcc
      301 ccaaattagc agggtttacc ttaaaaatca atgctgttgt caacctgtta agtttttctc
      361 tacacttaat ttttaaactg gttaagatac tatgtttaag aagttacaaa tcagactaaa
      421 taattttttc aaattcttgt atttatattt tatattgctg aaaattattt atttacatca
      481 aacatgtttt tgataaaaac atatcttatt agcataaaaa tttctattcc gttcataatg
      541 agtaagctcg gtatcctttg caaaatttac tattaatttc gaacagaacg gaacagatat
      601 ttgttttcta tacttttggc gactcaaaat ttcatttttt tatttattat tctttatgac
      661 ttttgacaaa ttattaaagc ccaattatct tttcgaaatc agttgggaag tatgtaacat
      721 gatcggcggc atttatactg ttatttcgac caaagctccc gccattaaag agaagttggg
      781 cgaccaatat attaccatcg gccctgatgt ttggaaagag acacgtaaaa atccaacttt
      841 taccgaagat aaatctctat ttgccgaatg gcgtgatagc gctgctgcta atggcattta
      901 tgttcgggtt ggtcgctgga atattgaaag tagccctatt gctatcctcg ttgattttac
      961 aaatctgttt cctgaaaaga ataacatttt cgctaaacta tgggagcgct atcagctcga
     1021 ttccattaat ggccagtggg attatataga accggctatg tttggacatg ccgtggggaa
     1081 agtaattgaa agtttttata actattacct ttcttactct tacaacgtcg ttgcccattt
     1141 tcatgaatgg atgaccggca ctggagtttt atatctgaac gaattcttac ctcaggttgc
     1201 aactgcattt actactcatg ccactactat tggtcgctct attagctcca acggcctgcc
     1261 attatacgga aaaataaatg agtacaacgg cgatgaaatg gctaaaaaat tagggattgt
     1321 cgccagatac tcgcttgaaa agaagagcgt agctaccgct gatgcatttt ctaccgttag
     1381 ttacatcaca gctaatgaag ccgaaaaact tcttggaaaa aaagtagatg ttgttacacc
     1441 taacgccttt tcaacatcac tcttccaaaa tgaagagaca tacagtaaaa aaagaaacat
     1501 tgccagagcg aaaatgatac aagttctgga ggctcttttt aatgaaaaga ttgatacaga
     1561 tgcctttttg acaattacca gcggcagata tgaatttcgc aataaaggca ttaatatttt
     1621 tattgatgca atcgagaaat tatcaacagc aaatcctgat aaacaggttg ttgctatttt
     1681 aaaaattcct gccaatcact ccggtccacg aaatgacctt ttgcagcgta ttgcaagccc
     1741 tgatttcggt aatccactgt caggagaata tttgacgcat ggccttcata atgcttctga
     1801 tgatgctatt ttaaacaaaa ttgcaaggac agggttaaaa aatactcccg gatcaaaagt
     1861 gaaaatagtt tttgtacctg cttacctcaa tggtgatgat ggtatttttg atatacccta
     1921 ttatgattta ttacccggtt tcgacctgac agtattccct tcatattacg agccttgggg
     1981 atacactccg ttggaaagca ttgcccacag agtgcctaca gtaacaacaa atcaagctgg
     2041 atttggtagg tgggttctcg ataatttccc ggaaaatcat tcggcggtag ctgtgctcaa
     2101 cagacccgac gatgacatta atactttgga tgctgaaatt tccgatatca ttctgaagac
     2161 catcagttta tcagatgatg agttaacggc attaagaaaa aacgcttacg aaattgcaac
     2221 taaagccaac tggaaagagt ttgtcaatta ctattacaag ttatacgata ttgcgcttga
     2281 aaaggttgat gagagagtag aaactttccc tgcacttcag atagtaaaaa gaaaagtaaa
     2341 acttactgaa aaaaccgtga ttgatactcc ggtttggaaa aaaattgtaa ttgaacctaa
     2401 gatacctgaa aagcttaaag ggcttcagaa aatttcgaga aatctatggt ggtcatggaa
     2461 ttttaaggca actgatatgt tcgaaaacat acatcctgaa aaatgggaag aagccggaca
     2521 aaaccctatt actttacttg attcactcaa tattgacgag tggaaagcat tggaaaacga
     2581 taaggacttt tacgaaaaat atttagagtc gtacaataaa tttgaagcct atctatccga
     2641 aagtgttgat aaatcaatag atgcagttgc ctatttcagt atggaatatg gtttacatca
     2701 cacgttaaag ttatattccg gtggattggg cgtattagct ggtgattact taaaagaggc
     2761 cagtgattca aataagaact tggttgcaat tggaatttta tataaatatg gctatttcca
     2821 gcaaagcatt tccactttcg gcgatcaaat agctgaatat cagcctcaga aattctccca
     2881 gcttccaatc gaaagagttt acggtgatga cggcaaacaa cttcatgtag aaatagcatt
     2941 acccggtcga acactgcaga tacgaatttg gcgctgtgat gtcggaagaa tacctttata
     3001 tttgcttgat actgacttta atgaaaatgt agaagctgac cgtaggatta ctcatcagct
     3061 ttacggtgga gatctggaaa atcgtcttaa acaggagctt gtacttggta ttggtggaat
     3121 cagagcgttg gaagttcttc aaatacaccc ggcaatttat catagtaatg agggacattc
     3181 agcatttata ggcctggaga gattacgtta tctgatacaa accgacaaat tgacttatca
     3241 tcaagcgctc gaaattgtac gcagttcaac cctatttacc actcatacac ctgttccggc
     3301 aggacatgat gtcttcgacg aagacctttt gcgaacctat ttgccacact atccggaaag
     3361 attagggctg acatgggatg aatttatgaa ccttgggaag tttgctgaaa atgatataaa
     3421 taataaattc tccatgagcg ctctcgctgc aaagctttcg caagaaataa acggtgttag
     3481 ccggatacat ggaagggttt cgcaagagat gtttcaaaaa ctttatgatg gttattaccc
     3541 atctgaaatc catgtgggat atgtaacaaa tggtgtacac ttgccaacat gggcatcaaa
     3601 accttggaag cagctttata aaaagatttt tgaagaagat tacaaacaaa aacagctcaa
     3661 ctttgatatg tggcaaaagg tacatagtgt tgaggatagt attgtgtggg aatcacgaaa
     3721 gcagctaaag tcagaattga ttgtgtattt gaagcaacgc ctgcgcgatg agatgactaa
     3781 aagacaagaa aacccaaaat taattttaca aaccattgat tcacttaatc caaatgcatt
     3841 gacagtaggc tttgcacgcc gatttgcaac atacaaacgc gccaaactac tcttttccaa
     3901 catcgaacgt ttggaaaaac ttgttaccat cagcgaaagg cctattcagt ttatttatgc
     3961 aggtaaagca catccaaacg ataaagaggg acaggatcta attaaaaaga tcattgagat
     4021 ttcgaaaaca aaaccattta ttggtaaaat aattttcgtg gaaaattatg atatgaacct
     4081 agcgcataag cttatttctg gtgttgacat ctggcttaac acccccaccc gtccacagga
     4141 agcctccgga accagcggcg aaaaagctgt tatgaatgga gtagtaaact tttccgttct
     4201 tgatggttgg tgggctgaag gatacaggga aaatgccgga ttcgccatac aggaagcaaa
     4261 aacgtatgcc aatcaacagt ttcaggatga gctggatgca gagataatat ataacctctt
     4321 cgaagataca ataatgccgc tgttttacga cttaaataat gataaaatac ctatagcttg
     4381 gataaaatat ataaagaata cggtatccga catagcgcct cattttacca tgaaaagaat
     4441 gatagaggac tattacagca aatactacta taagcttatt gaaagaacaa aacgaattga
     4501 aaagaataat tatcaacttg ctattgatta tgctgaatgg aagcggacaa tcagatcaaa
     4561 atggggtgag ctaaaattag agaaactgat ggtttctgat tccgatagtc caatttatct
     4621 tgaagacaac ttttcggcag aggtaacact cttcacaaat ggaataccag cgcaaaacat
     4681 aggagttgaa atcatctttt cgattaaaaa caataaaggt gcaagaaaga tactgctaac
     4741 ccgtgaaatg tcgttgctgg aagtaatcaa tggtaaagct ctttattctg tggatgtacc
     4801 agttgttaat gccggcactt tcaattatgg tttcagagta tttccaaaac atgaactaat
     4861 gccacatcgg caagactttc cccttgtaaa atggatttaa tacatttgtc agcaacttaa
     4921 aattaaaagc agacatgacc gacctaaata aaatttatac tatttcagga aaacctggtc
     4981 ttcacaaaaa tgtagccgta tcaaaaacgg gtttaatagt cgaatcgctt attgatggta
     5041 aacgttttaa tgtgtttgcc catgaaaaga tgagcgctct tggcgaaata agtatcttta
     5101 aagtgggagg cgatatccta ttaatagagg ttttaaaaaa gatcaaagaa aaatatgaat
     5161 caaagcctgt agcgaacgct gatttttctg gtcctcagtt aagagtattt atggaagaga
     5221 tacttcccga ttacgatccc gaacgagtat atacctccga tattaagaaa atccttagct
     5281 ggtataatct attagctgaa aaggatatgc tcgaatttaa ggaggatgag ccagaagaag
     5341 agcatgcttc agaacaaaat accgaaccca atactgacga atcagccgaa taaccataac
     5401 taattctttc tgatgtacaa actgctgatc agaccacttc tttttctgtt tcagcccgaa
     5461 accatacact cttttgtggt gaagggaatg aaattttggt ttaaaatccc gggaatagca
     5521 tgttttatca ggcaaattgc ctcagtaaat tccaccaaaa cagaaaaaga agtttttgga
     5581 ttaaaattta aaaacccaat aggtatcgct gccggatttg ataagcaagc ggagtttttt
     5641 gatgaattgg caaattttgg ttttggattt atcgaaatag ggaccgtaac acctaaacct
     5701 cagcctggaa atccactgcc caggatcttt cgactgccaa acgataaagc cttgataaac
     5761 agaatggggt ttaataatat aggcgtagat gaggcagtca aaaacttgaa aaaacgaaaa
     5821 acaaacataa taatcggggg taatattggg aaaaatacac ttacgcccaa cgaaaatgct
     5881 gttgacgatt atatttactg cttcgataag ctttttgact atgtggacta tttcgttgtc
     5941 aatgtaagct gcccgaacgt ttcggatctt agagaattac aagatcaaaa aagcttaatg
     6001 gaaatattgg ggaaacttca aagtataaat aatttaaaag agcgaccaaa acccatctta
     6061 ctaaaagtct cacccgattt gaactggcaa caacttgacg aagtgattga aattgtacac
     6121 aatactggta tctgtggtgt tgtagccact aatactacta ttacccgcga caaccttact
     6181 gcctctacta aaagagtaca ggaaattggg gatggcggat taagtggctt acctttaaaa
     6241 aatcgttcta cagaaatcat acgttatctt tccgaaaaat ctggaaaagc atttccaatc
     6301 attggcgttg gagggatttt tacacctgat gatgctttag aaaaaatagg tgcaggcgcc
     6361 gacttagtac aggtttatac cggttttatc tacgaaggcc catttatcgc aaaaaaaata
     6421 gtaagaaaaa taatttcaaa tcagatgaaa tgaccaaatc ggtggtaagc tcatggtcat
     6481 caataatgga gttcaaaatc atctgctatt tgtaaagcag gaataccgtt ggttttttat
     6541 ttaaatccgg cagattttcc ctccactccg aaatacttct cgatatgaca aactcctgtg
     6601 atgtagttag attgctggcg atacagagtt tcagatttat agaacaattt gaaattatgg
     6661 catctaaaag tttgttgttt cgaaaaggag tttcaataaa tatttgagtt tggttgttct
     6721 gaataattgt tttttcaagc tgtttcaatt tttgtttaaa gtcgtttcta tcaataggta
     6781 gataaccatg aaaagcgaaa ttttgaccat taaaacctga tgccattagt gcaagaataa
     6841 ttgatgaagg gccaactaac ggcgctattt gaaaaccgtg ttgatgaaaa atttttacaa
     6901 tccctgctcc tggatcagcc acacaaggca ctcctgtttc cgaaagtaag ccaagcgatt
     6961 tagttctggt atcaatttgc aataattcaa taatttcgtc tgagttatga tgcttgccaa
     7021 tttcgtagaa agttacttcg tgatcaaaat ccttagtgta tccggtttta cgaagaaaac
     7081 gacgtgccga tctaatattt tccacgataa attcatctaa agagtatatg attttaaata
     7141 cactctccgg aagtacttct ttggggttag tatcacttaa agttaccggt atcaggaaaa
     7201 gcttaggttt cattagttat gcttgttgcc atggtaaatt acttaagtca acgttaccgc
     7261 ccgagaggat tacccctatt cttttgtttc gacatcttat tttatcctcc atcagcgccg
     7321 caataggcac tgctgccgag ggctcaatca cgattttcat tcgttcccaa accaatttca
     7381 ttgcgctgat tatggcatct tcgctcgttg ttactatatc attaacgtat tttaaaatta
     7441 tcggataggt aatgctacca agcgaagtac gtaaaccatc agctatagta tcaggattaa
     7501 ttgaaggtac aagtgattta atatgaaagg agcgataggc atcgtcagcc tgttcgggtt
     7561 cagcagcgat tacgatagtt tttcggcaga aataacttgt agctaacgct gttccgctta
     7621 ataatccacc gccgccaacg ggtgcaagga taatatctaa gtcagggtaa tcctctaaaa
     7681 gctcaagtgt tgcagtggct tgcccctcta taactaaggg ttcattataa ggatggattt
     7741 ccactgcgtc ggtatcttca ataagagagg caagggtaga ttctctggat gccagattgg
     7801 gttgacagaa agtgattatc cctccatatc gttcaacagc agcaatcttc acttttgaag
     7861 agttgttcgg catcactatg taggctttag tccctctcag cttggcggca cgcgatagag
     7921 cagcagcatg gttacctgaa gaatgagtgg ctactccttt aattatgcta ttgtcgtcta
     7981 aagcaaatac ggcattgcaa gcgcctctcg atttgaaaac gcctgccttt tgcatgttct
     8041 cacatttgaa aaacaactgg caatcaaaga gttttgaaat gctgcttgac gttaaaaccg
     8101 gtgtgcgatg aatgtatggc cttatacgac ttgctgcagt caggatatca gtggcagtag
     8161 gtattgtttt ggagtgcaca aaaaaatgat caaaggtttt ctaattcatc ggcaatcttc
     8221 tcacaggctt cgtccaacat attgtaaacc aattcaaatc cttctttatc actataataa
     8281 ggatcaggaa cttccatact ttttcgatct gagatcacac tcataatcag gcggactttt
     8341 ttataatctg attccgttct tgctaatcta agtacacttt tcatattcga attatccata
     8401 acgaagatca tatcatattt atcaaagtca tcggctttaa attgtgttac cctatgtgta
     8461 gtgatatcaa ccccatggtg gcgcgcaata gctattgtcc ttggatcagc aggttctgat
     8521 atatgataat tcgatgtgcc cgaagagtat gtttcagcat tgagcgatct tgcgttgatc
     8581 ttttgctgca tgattccttc ggctaatggc gaacggcaaa tattacccag acacaccatt
     8641 aaaatttttg ttttaggttt tgaccccatc agtaacaaat tcaggactaa tgttttatga
     8701 tcctttcaag ctcattgaaa taagacttga attttttatc tgtttcgacc aaatttctta
     8761 ctgtacggca ggcatgtaat accgtagcat gatctttatt tccgcaatgc aacccaatag
     8821 tagtcaggga agcctttgta tgttctttgg caaaatacat agctaactgc cttgcttgca
     8881 ctatattgcg tctgcgggtt ttggaattca tctcctcaac ggatagatca aaatattgag
     8941 aaacaacttt ttgtatatac tcaatggata tttccttatt tgtacttctg ataaaattat
     9001 ccacgatttg ccttgcgagg tcaatgttta tctctttctt gttcagcgaa gcttgagcaa
     9061 gcaatgatat taaggcgcct tccaactctc tgatgttact tgtgatgcta taggctaaat
     9121 attctattac attatcaggc atagtgatgc cgtcgttata aagtctcttt tgtaatatgg
     9181 caacccttgt ttcaaggtca ggagtttgaa gatcggctgt aagcccccat ttaaaccgtg
     9241 aaagtagcct cgactcaatg ccttcaattt caaccggcgg gcgatcggaa gttattataa
     9301 gctgttttga tttttgatgt aactggttaa agatatgaaa gaaaatatct tgtgttttct
     9361 cttttcctgt taaaaagtga atatcgtcta tgataagtac atcaataatc tggtagaagt
     9421 ttgtaaagtc gttcacatta ttatttctca ccgattcgat gtattgagcc atgaactgat
     9481 cggtattaac ataaagtacg gttttttcgg ggaaattatt tttaacttcc agtccgatag
     9541 cgtgtgctaa gtgtgatttt cctaagccaa ctttactata aataaaaaga gggttaaatg
     9601 aggttttacc tggatttttg gcgatagcca gaccggcaga acgcgccaga cgattacaat
     9661 ctccctctat aaaattatca aatgaatatc tctcaataag ttgcgagtta atttttacct
     9721 tttgaagtcc tggaataaca aatggatcag gaataatttt agtagttcct tttaaggtta
     9781 aaggtagtga tacaccggga tttttggttg ctgatctgtt ggaagtcggc atgattatgg
     9841 aaaatggatc ggtattatta ccggtgttat caataagaac gctatattcg agcctccctg
     9901 aagtgccaag ttccttttta atagtctttt ttaacaggtc aatgtaatgc tcttcgagcc
     9961 actcataaaa aaactggctt gggacctgaa ttgtaagtac attgtcaacc aaccttaatg
    10021 gcctgatcgg ttgaaaccat gtctcgtaac tctgatgatt gatattgtct cttattattt
    10081 tcaaacaatt atcccaaact ttttgtgcag acttctccat aatacatttc taattaatag
    10141 ctgatgatca aatagttatt aagcgatttg ataattggtt caatgggttt gggaagaacc
    10201 attacccgaa ataattttca acaaagatga acaaaaatta gttgataaaa aaatttatat
    10261 tacttgtttt tgtcaaaaat tattctaata gggttcgagc caactctcct aagaatcctt
    10321 acaactattt gtttgtgact aataaatcga gttaattaga catattttaa attacatttt
    10381 taacctaatt ttaaaaaatt gctcaagata aaaatttgag aatttaagta cttttattta
    10441 gttattttac taagtccata tttaggaaag taaatggttt actataataa tcttttaact
    10501 cggatacaga ttttgacaat gctttaacgt tactttattt gtgatcagga caataatagt
    10561 tgtactgaat atacactaaa tcaggtctat tctcataaaa ttattagttc aatgagcaag
    10621 agctttaaat tgattaatct aagtttaatt cataaaaaac tgggagtctt acaccagcga
    10681 tatgcattca tttgcaaccg atagtgttag gggacaaact tgttacctca ttattgatat
    10741 tgagcctttg aatatgtcgg ttccaaaata gcccttagcc ttgagaacat aaaaatagac
    10801 accatcttta agattatctc catcccaatt gccctgataa ttattggttg aatagactct
    10861 ttttccgtat cggttaaaga tcatcaattc attactcatg taatattcga gatctttaat
    10921 agtaaatatt tcgttgtagc cgtcgccatt tggtgtaaat acatttggta tggaaatttc
    10981 agcctgccca acatccactt taagcattac agaatccacg cattcgttgc tggtagtgta
    11041 cttcagttta acttcatact cccttacttg atcaaagact ttttcggggt tctcttttgt
    11101 cgaaaaagta gtatcgccaa aattccagtt ccaatcaatg atttgtatgc tatcaatgga
    11161 tgtattttca aacgaaaatt gtactaccgg attctggata tagatttgtt cttcggggtt
    11221 gcggattata ttaactatgg gcatatcaaa cactttaacc ggtaccaacg aatctctgac
    11281 gcaaccatac tcatcggtta cttcaattct atatagcgct tcgcataatc caaaagctat
    11341 tgaatcggat tgaactggcg aagcattagg ccaataataa ctgtatggtg gtaaaccccc
    11401 cgataccaac acttgtagct gtcctttaca agtacccggg caccctgtgt taatctgatt
    11461 cacttcatca aaatagatgc ggggatagag gaaaatctgg acactatcca tacaagtcaa
    11521 cacattcaag ctgtctgtaa tatgaacata aacggtcgta gtgtcaaggt ttggaacaaa
    11581 ttctacagcg ctgcggtctg gtttgctaaa aacaagatcc ggatcgcttg ccgaccattg
    11641 atactgtaac gtatcagtat taattgacaa aattaccggg tataaatagc aggtttgaat
    11701 tgtatcaggt tgctctatcc atgcattaaa aaacataaca aaggttgagt cttgaataat
    11761 tatcagatct tcggtggtac accacacaaa ataccaaccc ggggttttcg cataaatggt
    11821 ttgtgtctgc tcgtcagtat tccacaaata actaagaaac cccggacctg cgtctaaaag
    11881 gacgctatct gccctacaag caaatgtaga atcaggtaat tgaggaagct gagctgacaa
    11941 ctgacctgta aaaagcaaca ctgaaataac agatataatt gagagtctta cacgcatcta
    12001 attcaaatgg ttgatcattt cgttttaatg gcggtgcgaa aatactgata attcattatc
    12061 caaaattaca attaatagat acccttcaat agcattaaaa gggtacactc ttcctgtgtt
    12121 tctatacctt tttgtttagc caatgaagat aattcagcgt tttcggtgcc tggattaaaa
    12181 attattcgtt tgggggatat cgtatttaaa atataatcgt acatatcagg ttggttttca
    12241 ggagcaaggt acaaagtaac tgtatctatt tctgatacag atggattatc aatggctatt
    12301 gcaatgttac cgatatttcc tacacgttgt cctacagcta ctaccggatg cccagccctt
    12361 accaaattat ttaccgcttt aaaagagatt cgtgaaggat taggacttgc tccaagaaca
    12421 agcgtctttt tcaattctgc catgtatttt gattttgtag gtttgaaatg cttcagcagg
    12481 acaaattatt gttttctaaa gaaatttttt attttaaaac aaagtaaaca aaatgacaaa
    12541 attgttgaca tcagatcagt gtattgcatt attttgccaa aaagtcatta ataatgagat
    12601 agttaagtga caatatttca ttttacaaaa gataaaaagc tctggcaata ttgttgatac
    12661 cttgccgctc ttagaagaac aaaaatcaaa aagaataaca atcatgaatt taaataattt
    12721 cacattaaag gctcaagaaa gtattcagaa agctcaggag attgctgtcg gaagccagca
    12781 ccctcagatt gaaaatgcac atattttaaa aggacttctc atttcagacg acaatgtagt
    12841 tcctttttta ataaaaaagc ttgatgtaaa tctcactcac cttcagtcgg agctggacaa
    12901 gctcctgaat gggtatgtta aggtttccgg cgggcaaatt tatttatccc caaatgctac
    12961 tacagctttg cagaaatcac taaccttcct aaaggatttt aatgacgagt ttgtaactgt
    13021 tgaacatatt ttattgggta ttttatcagt aaaggatgac gtgtcagacc ttctaaccca
    13081 gcacggtgtt acgttacaaa aattaatacg cgccataaaa gaactcagaa aaggctctgg
    13141 agtaaaaagc cagactgccg aaagctctta caatgcgcta aaccgttttg cgaaaaacct
    13201 taacgagctt gcggctgcag gaaagctcga cccggtcata gggcgtgatg aggagataag
    13261 gcgcattttg caaattctat cccgacgtac taaaaataat cctgttttaa taggtgagcc
    13321 aggtgtgggt aaaacagcca ttgctgaggg catagcccac cggattgtaa atggcgatgt
    13381 tcccgaaaat ttaaagtcaa agcatattta ctcattagat atgggggcgc ttatcgctgg
    13441 agccaagtac aaaggtgaat ttgaagaacg gctgaaaagt gtgataaaag aggtaataga
    13501 atctgatggc gaaattatcc tgtttattga tgaaatacat acattggttg gagcaggcgc
    13561 cagcgaagga gctatggatg cagcaaacat actaaaacct gctcttgcac gaggcgaact
    13621 aagagccgtt ggagctacta cgctaaagga atatcagaaa tatttcgaga aagacaaagc
    13681 gctcgaacgc cgatttcaga tcgtaatggt aaatgaaccc gatacaatgg acgccatttc
    13741 tatccttaga ggcctgaaag aacgatacga aacgcaccat caggtaagga taaaagatga
    13801 agcaattatt gctgcggtag agctttcaca aagatatatt tccgataggt tcctgcccga
    13861 taaagccatt gacttgattg atgaagcagc ttcaaaatta agacttgaaa ttaattcgtt
    13921 gccggaagag cttgacgaaa tagagagaaa gattaagcat cttgaaatcg aacgggaagc
    13981 cataaagcgg gaaaaggata aagagaagct ggcagttctg tcccaggaaa ttgctgatgt
    14041 tacagagaaa cgtaaccacc tgcgcaccaa atggatggct gaaaaggaag ttgttgatgg
    14101 gatacagaaa aaaaagagag agatcgaaga tcttaaacta aatgccgaac aagctgaaag
    14161 ggatggagat tttggtaagg tggccgaaat aaggtatgga agaatcaggc atgcggatga
    14221 ggagttggaa aaacttaagg caaaacttac ctcaatacag ggcgacagcc ctctgataaa
    14281 tgaggaggta gattccgaat gcatcgctga tattgtagca cgctggacgg ggattcctgt
    14341 ttcccggatg ttgcaaagcg aacgggaaaa gttacttcaa ttggaagaac atcttcatga
    14401 gagagttgtt ggtcaggatg aagcaattga agctgtggct gatgctatcc gccgcaatcg
    14461 agcaggatta caggatgccc gaaggccaat aggatcattc atatttttag ggactaccgg
    14521 tgttggtaag actgaattgg caaaggcgct ggctgaattc ctgttcaacg atgaaaattc
    14581 aatggtaagg ttggacatga gtgaatatca ggaacgccac acggtttcaa ggctgatagg
    14641 agctcctcct ggctatgtag gctatgacga aagcggccag ctcactgagg ctgttaggag
    14701 aaagccttac tcagtagtgc tctttgatga aatagaaaaa gcccatccag acgtctttaa
    14761 tatcttgctg caagttttgg acgacgggag gcttaccgac aataaaggac gaacggtgga
    14821 ctttaaaaat tcaattataa tcatgacttc aaatattggc tcacatcaga ttcaggaagg
    14881 gcttgggaag ggtatcaata ccggagatga ttctaatttc gaaaaagtgc ggctcgaagt
    14941 ctttgaacta cttcgccgaa ctatacgccc tgaatttctt aatcgggttg acgaaattat
    15001 aatgttcagg ccattatccg aagatcagat caacgaaatt gcgcagttgc aagtaaatat
    15061 tttaaaagag aagttggaaa aagttggaat agatatcaca gtaactcctc aagccgtcgg
    15121 ctggatcgca cacgccggct tcgatccgca atatggcgca cgaccagtga aaagagtaat
    15181 tcaaaggcaa ttgatgaatg agctatcaaa aatgatactt gctggaaatg ttagtaaaga
    15241 tgaatctatc gtcattgatg cagataaaaa cgggttgaca ttcgctaaag agaactaaaa
    15301 gttttattga ttagtacaag atgaaatagc cctctgatta aggcctgagg gctttttttt
    15361 tgacactgtc ccaataattg tgttccgcag ttaaatttct tcaatcattt gtctctttaa
    15421 attttgaaat ggttttatca atgtgattaa gttcaacaat ccactctaat aaatctttat
    15481 ttaaccactc aattccttga atgatttctt ctttttacgg gttcaaaaca ttcagatcca
    15541 aagtaattgc ttcgtaatga aagatcctat ttcttagttt tataattccg ttagaacttg
    15601 gaacttattg tcttcctttt tctctaagta gatattagtt cttgcttgtg atattctatc
    15661 aatttgaaag ttggtagcaa tttttacaaa atcattattt tcataccata ctctatcgtt
    15721 aaactttttg gttaattggt aattaatgga gtttcttaac ccaatttcca atatagcaag
    15781 taaagggtaa atagattcgg atataagaat gttgcccttg taattagcaa ttgctttaga
    15841 aggatcattt ttgtgatagt ttaaaaaggg ttcaatcctt tccctagaaa taattctttt
    15901 ttcaattatt gttatccata attgaatttt tacgatgtag tcctagttat ttctcttcta
    15961 atttttatgc tggtgatgaa gctgggtttt ttgtttttta tcgtcaaaga tagtgtgttc
    16021 cccattttaa atatctcatt tgttttgtag ctataatcat aggatagatt caattaacaa
    16081 attagcaatt taaatatcag ttttgttttt attttcaaca cttctaaaat ttatatcatc
    16141 actaatcaat tcggtaaagt aaaatagata attttgtttt gatttttatg taaatagcct
    16201 cttgttagtg aattctttca aatcgtcgtc ttatgcttga tagaattatc ggtttcagtc
    16261 tgaaatacaa gttgatagtc attcttttca cactgactat tgttgggttt ggaatatatg
    16321 ctattttcaa tattcctgtg ggagcagtac ccgatattac gaataatcag gtgcaggtca
    16381 taaccacctc aaacaatctc tccacgcagg aaatagagca atttattaca gcgcctgttg
    16441 agcttgaaat ggcgaacctg ccaggcgtgc aagaaatccg ttccgtttca aaattcggac
    16501 tttccgtggt aaccattgtt ttcaaagaaa gtatgggaac ctatttgcca cgggttctta
    16561 ttgccgaaaa aatcaagact gcctccgccg atattccaga aggttttgga actcccgaaa
    16621 tgggacctat aaccactggg ttaggtgaga tttaccagta cgtgcttgat gtgaaacccg
    16681 gatttgaaga tcgttacaca gctatggatt tgcgaactat tcaggactgg attgtgcggc
    16741 gccagctttc cggtatcgat ggtgtggtgg aggtaaatag ctggggaggt tacttgaaag
    16801 agtatgaagt ggccgtcaat ccggctcgtt tgcaagctat tgatgtaacg ttaatgaatg
    16861 tctttgacgc tttggaaaaa aataacagca tttccggtgg ggcgtacatt gagaaaaaaa
    16921 gtgaaagtta ctttatccgt ggcgatggtc aggttaaatc tattgaagat attgagaata
    16981 tcttggtgaa aaataagggc gggatccctg tactggtaaa ggatgtagca aaagtgcatt
    17041 tcggctatgc aaatcgtttt ggctccatta cagccaatgg cgagggtgaa aaagtactcg
    17101 gacagattat gatgctgaaa gatgccaact ccaaaaaagt aataaacgaa gtaaaagcca
    17161 gggtggcaga ggtgcaaaaa actcttcccg aaggggttta tatcaatccc attttagagc
    17221 gaagtgagtt gatcagtaaa acaacgacaa cagttttaga aaacctaatt ttcggctgta
    17281 ttattgtctt ttttgtggtt ttaatgcttt tggggaatat gcgttcggcg ctggtaatcg
    17341 cctctatgat acccttatca ctccttttta ctctttctat gatgtttatt tttggtatag
    17401 atgccaacct gatgagtctt ggtgcactcg acttcggaat tatcattgat ggagcggtaa
    17461 ttatcgtaga atatgtcgta gtacggctaa cagccaacca gttacagttt cggaatactt
    17521 ctcccgaaga gaggttgaag ttgaacgacg aaatcacgaa agagagtgcg tcaaccatga
    17581 tgaaatcagc cattttcgga cagtttatta ttttaattgt ctttattcca attctttcac
    17641 tacaaggtgt agaaggtaaa atgtttcgtc cgatggcgtt ggcgtttggt tttgctataa
    17701 tcggagcaat gattttctgc ctgacttggt tgcctgtgat ttcttccatt ttcttacgtc
    17761 ctgttgatgt aagtaaaaag aatttttcgg ataaaatcat cgaatttgcc caaaaatctt
    17821 accaacccgt tttgaaatgg gctttgaata aaaaagctct tgtgttcaca ctctcattcc
    17881 taacgttgat actatcagga ctgattttca acaggatggg aggtgaattt attcccacac
    17941 tcgacgaagg tgatt
//
LOCUS       DAOOAI010000043        17815 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_4170_length_17815_cov_5.283727, whole genome shotgun sequence.
ACCESSION   DAOOAI010000043 DAOOAI010000000
VERSION     DAOOAI010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17815
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4170_length_17815_cov_5.283727"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="PK345_04480"
     CDS             complement(<1..797)
                     /locus_tag="PK345_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34309.1"
                     /translation="MTSEKFYSKALEANLAETRYKDIVIPKHLQDFIALSAKYYGINK
                     RAQECIIEYLHPFSNRLFVVEQLREILISDFWFYSSLPEANNAFSVLIMLYNEILTTC
                     EQEEIGKIIVKTLLEFINLIVKEKKTELFPAIHHSLKALYDNLDNNEKAYIINTRYFA
                     KFLDTVIVIPEFEEEVITLTKDVYRNNLMFWRESTQIESWYEEKKDLLVDDFKTIFVK
                     AGDDWFNELLGRLNAADTWEKLNTGFPGFDDMADHFAALVNNLDSFI"
     gene            complement(1107..2549)
                     /gene="dacB"
                     /locus_tag="PK345_04485"
     CDS             complement(1107..2549)
                     /gene="dacB"
                     /locus_tag="PK345_04485"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF014200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004175 - endopeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine
                     carboxypeptidase/D-alanyl-D-alanine-endopeptidase"
                     /protein_id="HPX34310.1"
                     /translation="MSIFINGGCTSAGSKMSAQQPDSTKNLLEKFLSSKSLAHSTVAI
                     KIIDLQSNEVLVSHNESLTVVPASVQKIFITAAALETFTPYHTFSTILSYSGEIDKGD
                     VLHGDLYIIGGGDPTFGSPYFGDNYGNVINRLSDATKAAGIKKINGNIIGDASIFGEP
                     NIPGTWIWEDIGNYFGAIPSGLNIYDNSYEIAFRSKKKPGTPTTILRITPEITGLAFE
                     NHVISAKDNRDNAWIFGSYLDNKRVITGAIPAGKENFIIKGAIPDPAKLVTEQLAEKL
                     KTNGIAITGNTHSSFSASSKIQKRTLLEIKSPNLADIINKTNSKSVNLYADVLLMHLA
                     ATAGKPSLTEGGKILKSFWKDKGMHSEGIFLMDGSGSSRSNGVTADQIVFVLKYMNDS
                     HYSEIFQESLATAGVSGTLRNFGKGSSLESNFYAKSGSMARIQSYAGYLTVASGKKLA
                     IALIINNFDDEPATIKNQIKNFLIEVYQHL"
     gene            complement(2613..3431)
                     /locus_tag="PK345_04490"
     CDS             complement(2613..3431)
                     /locus_tag="PK345_04490"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: protein motif:HMM:NF003742.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HPX34311.1"
                     /translation="MPKIFIVSDGTGRTATQALNAAITQFPGHNLETIIRNDVRSKEQ
                     IEEAVKEASETKAFIVHTLVSNSLRDFIITTGRTYNVETIDLMGPLLARLSEKLMHSP
                     TEKPGLFSEINKEYFKRIDSMQFAFHHDDGQHPEELHKAEIVLLGVSRTFKTPLSIYF
                     AFKGWMVANVPIVLNQPPPNEIFQIPPERVFCLTAYAYNLSTLRQVRLEHLGSGAANY
                     ATLQHVNMELNYARFIFSKQPLWPVITVTNKPIEEIASEILAIKKAKIASLETD"
     gene            complement(3434..3943)
                     /locus_tag="PK345_04495"
     CDS             complement(3434..3943)
                     /locus_tag="PK345_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34312.1"
                     /translation="MKRLLVKIMILSILFQLTAVASAQQYFVVQKKGKFKNFKYISGD
                     YIYLETKRGEFKLSGKITQINDTSLVINKTSEIGLNDIGVIFRPNGLIRGFSNLFFIQ
                     GGAAYFFIAGANALINNESPIIDKSTLLISGSMIATGFAIKPFITRKLNVKEKWEVKI
                     LNFDEFKKE"
     gene            complement(3978..4259)
                     /locus_tag="PK345_04500"
     CDS             complement(3978..4259)
                     /locus_tag="PK345_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34313.1"
                     /translation="MANIVSVNISEKKGTVKTTVSEIILNEYRVVGDAHVVDCNRQLS
                     LLAVESVKKWSKKSGSKVSFGVLAENITPEGVQRSEVHILSLLLSIICS"
     gene            4349..4687
                     /locus_tag="PK345_04505"
     CDS             4349..4687
                     /locus_tag="PK345_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HPX34314.1"
                     /translation="MDGIKEIINHIVAFRDERNWEQFHNAKDLAVALSIEASELNELF
                     LWKSPAETGNIEREFVADELADVLMYALLLADRFKFDVKEIILDKIKKNALKYPVEKS
                     KNLALKYTQL"
     gene            complement(4705..5445)
                     /locus_tag="PK345_04510"
     CDS             complement(4705..5445)
                     /locus_tag="PK345_04510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34315.1"
                     /translation="MKTKSLIKKFGILFLVLLLIQSLHLLHGQNPIKKGTPLSKDLVI
                     DIQPVVVPNLPESSGATYYPTVLPGEPGDAYLDVNWNSGLVIMKDQSEIELPQMRYNI
                     LTQQIQFVRDTEIQAISNPQEVSLIRINDKVFIYNEFITDGVRRSGYLELLEDGSCKL
                     LRRWIASYKQVDELTGEETIYRTQRYFLQFDGGEIGEVKTSSAAFCETFGKHGNELKK
                     FMKEQKLKVRNPQHLRSLVSYYNMIATK"
     gene            5696..6373
                     /locus_tag="PK345_04515"
     CDS             5696..6373
                     /locus_tag="PK345_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPX34316.1"
                     /translation="MKIVVSESVKYQEFINQTAYYVKDRLRGCEGGHDWWHVYRVWRM
                     AKHIAVKEKVDILVVELAALLHDIADSKFHNNDEEIGPYLSEQFLLKIGVEKATIEHV
                     VSIIRNISFKNSFDKPAFYSKELAVVQDADRLDAIGAIGIARSFNFGGFKNRPLYDPD
                     SPPKTKMTKEDYQKSDSPTLNHFYEKLLLLKDMMNTTTGKKIAKDRHRYLVKYLERFI
                     KEWEGKL"
     gene            complement(6538..7053)
                     /gene="cobO"
                     /locus_tag="PK345_04520"
     CDS             complement(6538..7053)
                     /gene="cobO"
                     /locus_tag="PK345_04520"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPX34317.1"
                     /translation="MSKGKIHIYTGNGKGKTTAAIGLAVRAAGAGKRVFIGQFVKGMH
                     YNELNALKLIPNITIRQYGLDCFIKNQPTDKDIAAALKGLEEIKTITTSKEYDVVILD
                     ELCIALYYKLFDEEEIIKLIRSKPESIEMIITGRYASERLISEADLVTEMKEIKHYYN
                     NGQSARKGIEY"
     gene            complement(7053..7913)
                     /locus_tag="PK345_04525"
     CDS             complement(7053..7913)
                     /locus_tag="PK345_04525"
                     /inference="COORDINATES: protein motif:HMM:NF018290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX34318.1"
                     /translation="MELAIISGKGGTGKSSISAAFTSMQQPVMLADCDVDAANMFILF
                     NPTHEEEQVYIGGEKAVIDYDRCNNCGLCISYCRFDAIKCVNNIVHILEEVCDGCKLC
                     SRICPFEAISIVQNDKSRMYSGSFRYGKMVYGRLAPGEENSGKLVNIVRAKAREIAKK
                     HNIENIIIDGPPGIGCAVVSTITGTDHTLIVTEPSASALHDLKRTIDICNKFNLKIWV
                     TINKADINPDMTEHIIQYCKENNIAIAGLIPFDKSVVEAMVQCKTIMEYRPESLAAKA
                     IITIYNNILG"
     gene            complement(7894..8760)
                     /locus_tag="PK345_04530"
     CDS             complement(7894..8760)
                     /locus_tag="PK345_04530"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX34319.1"
                     /translation="MKVAIASGKGGTGKTLLSVHLFDNLMQSGEQVVLVDCDAEEPND
                     MIFFDGKLIDEAEVLQQTPIIDTDKCTFCGKCHEYCNYNAIFYLPPASMIHVIDELCH
                     GCGACNVACKFGAITERPLSLGVVRNYQIRPNARIVESRIKVGVMTPVPLIKAGLKQI
                     RNEPFVLLDSPPGTSCPFIHTILDADYVILITEPTPFGLSDLKQSVETLKTVGKAYGV
                     IINKAGIGDSNIYDYLKNENIELLMEIPFDKEIAYHYSKGELLTEYNPKLQSDLNNLL
                     NNIHKKYGTSNN"
     gene            complement(9039..9407)
                     /locus_tag="PK345_04535"
     CDS             complement(9039..9407)
                     /locus_tag="PK345_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002562098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPX34320.1"
                     /translation="MNRKIAIPLENGVLSQHFGHCQKFAIVEVVNNQITDIVETTPPE
                     HVPGLYPRWVAKFGVTDVIAGGMGQKAIHLFNEQNINTFVGAPVKTARELVEDFLANK
                     LELTANYCDHKEHKPHGNCS"
     gene            complement(9487..9609)
                     /locus_tag="PK345_04540"
     CDS             complement(9487..9609)
                     /locus_tag="PK345_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34321.1"
                     /translation="MSNLHQEETAIEEVWGLDAVVAEEQECSADFVEENNRIDL"
     gene            complement(9713..10027)
                     /locus_tag="PK345_04545"
     CDS             complement(9713..10027)
                     /locus_tag="PK345_04545"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPX34322.1"
                     /translation="MKIAITSTGNNLKSNVDSRFGRCAYFVIYDTVSQVTEFIPNPNL
                     NVNEGAGPSSVQLVAKHKVSKVFSGEFGGKVKSLFDSLNIQMVVIKENKTIEEIIEMQ
                     NL"
     gene            complement(10099..10800)
                     /locus_tag="PK345_04550"
     CDS             complement(10099..10800)
                     /locus_tag="PK345_04550"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018358094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA];
                     GO_process: GO:0098869 - cellular oxidant detoxification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HPX34323.1"
                     /translation="MEQEIFAMPRIGDNAPAFKAVTTQGEVNFPSDYKGKWVILFSHP
                     ADFTPVCTSEFMTFANMEAEFKELNCELIGLSIDGLYSHIAWLRSIKEKIEFKGMKNI
                     EVKFPLIEDITMEVANKYGMVQPGESNTKAVRAVFFIDPVGKIRAIIYYPLSLGRNFK
                     ELKRVLIGLQTADKFNVATPADWHPGDDVIVPTAGSCGTAQARVEGKEGDMKCYDWYF
                     CTKPLSKEEVFKGIK"
     gene            complement(10837..11160)
                     /locus_tag="PK345_04555"
     CDS             complement(10837..11160)
                     /locus_tag="PK345_04555"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HPX34324.1"
                     /translation="MKIAIAATDNHIDAFVDRHFGRCEWYCIFDTQTNNYEFIPNTAF
                     NLTKGAGYKAIDLLFGKSINVVIAGRFGSRAIETLRAKDIQMIIPENQITINEILKNC
                     KQNSN"
     gene            complement(11165..11560)
                     /locus_tag="PK345_04560"
     CDS             complement(11165..11560)
                     /locus_tag="PK345_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006798301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HPX34325.1"
                     /translation="MGRCKKNRFIQRAPHFHGFTTRGLKWKHDTDVFLNYEEYEAINL
                     CDYELLTQEEAARMMKVSRPTFTRIYESARRKIAKAFIEGHDVRFIGGNVEIISWYKC
                     DTCKISFTLQKDSPMACPLCKNELITESH"
     gene            complement(12248..15502)
                     /locus_tag="PK345_04565"
     CDS             complement(12248..15502)
                     /locus_tag="PK345_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HPX34326.1"
                     /translation="MKKLFFTVIFFSMAALTVTAQHETRLMRFPTIHGNHVVFSYAGD
                     LYTVQKSGGIARQLTNDKGNELFPRFSPDGKYIAFTGQYDGNTEVFVIPAEGGVPRRV
                     TYTATLQRDDVSDRMGPNNIVMTWKDNNTIIYRSRKKSFNDFIGSLFEVSINGGLSKQ
                     VEVPEGGFCSFSADGKKMAYNQVFREFRTWKYYHGGMADDVWIYDFETKNTVNITNNK
                     AQDIIPMWHGNSIYFLSDRDRTMNLFAYNTKTQQVTKLTNYSDYDIKFPSLGDNAIVY
                     ENGGFLYYFDLTTQQPVKIEIQILNDFQASRPQWKQADKTIRAASLSYDGSRIAFSSR
                     GDIFNVPAKKGITKNLTDSNGEHDRAVEWSPDGNWIAFISDKSGEDEIYIMKPDGIEK
                     PIQLTKNADTYKFKINWSPDSKKIAWHDQLKRLLFVDISSKETTLVASSESNEISNYD
                     WSPDSRWLSYVFTIPNEVSRIYAYNLETKTSTPITDSWFNSSSPSFSPCGKYLFFSSM
                     RSFNPIYSWTEWNHVYADMVKVYLVTLDKNTTSPFEPKDDTPEVKKDEQPAKDKKEEK
                     AVENDQKNVNIDFDGIYDRVVEITPESGYYWNIKGVEGGIYYIQSKMGSNSSKLIFFK
                     FEDQKETEIGSNMNFTISGNGKKMLINSMGKYTVTDLPKAAVKIDEAVDLSGMKIWVD
                     PKAEWQQIYDESWRQMKYFFYDPGMHGVNWDKMHEKYNLLLPHVNSRYDLSYVIGEMI
                     GELNVGHAYVNDGDLIKPERIATGLLGAELSHHKSDFYQIDKILKGQNWNKNIRSPLM
                     EPGLGIKEGDFIIAVNGQTTKEMNDIYASLVGKSGTTVEISVNSLPSESGARKVLIQP
                     IANESELYYYNWVHDNIDKVNKATNGEVGYIHIPDMGPGGLNQFVRYYYPQLRKKALI
                     IDDRGNGGGNVSPMIIERLRRELSMMAAGRNSVSYSKPDGMMAGPLVCLINRYSASDG
                     DLFPYQFRKHNLGPLIGTRTWGGVVGIRGSLPFIDGGSLMKPEFAHYSADGKEWIIEG
                     TGVEPDIEIWNDPALEFSGVDQQLNKAIEVILELLKDYPDPQYPIAPPFPDKSK"
     gene            complement(15529..15780)
                     /locus_tag="PK345_04570"
     CDS             complement(15529..15780)
                     /locus_tag="PK345_04570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34327.1"
                     /translation="MKRYFIIIGALKWLGKALAEVMLDKRHVLFLISRSPHKDISKKA
                     IHKKCQIYRILYDLSVINAKKGIVGPLSMSNIEITAFIL"
     gene            complement(15820..16713)
                     /locus_tag="PK345_04575"
     CDS             complement(15820..16713)
                     /locus_tag="PK345_04575"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPX34328.1"
                     /translation="MLSKRLNLRNITVLILIISFFGPRLIAQIKIPDDFCITSNELKL
                     FNLINNYRKEYNLPEVSLSKNLCFVAKLHVNDLQTNRPDVGNCNLHSWSDQGIWTECC
                     YGSESLNNTCMTSKPAELTTYNGKGYELAFWESLEAVPEIVLDSWLSSIVSKEMILNQ
                     GAWKNYNWKSLGIGIYKGYAVAWFGVEGDPDKSVNVCKAKEPKALLERILPTNEQEKT
                     STKAGQEVRYYLIISSVKDKNQAKKEVETLKAKGFKNPSVVASETNYRISLGNYSTRE
                     QALEAKNKLGERYKAAWILKQ"
     gene            complement(17074..17571)
                     /locus_tag="PK345_04580"
     CDS             complement(17074..17571)
                     /locus_tag="PK345_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HPX34329.1"
                     /translation="MEKKKEMVISVLKGLAIAYEIYEHPPAPTIEEAKKHWKFIEATH
                     CKNIFFRNHKGNRHYLVIIEHTANLAIHDLEKRLRQGKLSFASPERMQKYLGISPGSV
                     SPFGLINDKDNHVHIFLDENLKTASKISFHPNINTYSVIIAFKDFIRYLDWTGNSYEF
                     LQLYD"
BASE COUNT         5108 a         3334 c         3224 g         6149 t
ORIGIN      
        1 tcaataaaac tatctaaatt gttgactaac gctgcaaaat ggtcggccat atcatcaaat
       61 cccggaaagc cggtgttaag cttctcccat gtgtcggctg catttagcct gcccaataat
      121 tcgttgaacc agtcatcacc ggccttaaca aaaatagttt tgaaatcgtc aacgagcagg
      181 tctttctttt cttcatacca gctctctatt tgtgtgcttt cgcgccaaaa cattaagttg
      241 ttacgataaa catcttttgt aagtgtgata acctcttctt caaattcggg aatgacaatt
      301 acagtatcta aaaatttagc gaaatatctc gtgttaatga tgtaagcttt ttcgttgttg
      361 tcaaggttat cgtaaagcgc tttaaggcta tggtgaatgg ccggaaaaag ttctgttttt
      421 ttctctttca ctatgagatt aataaactca agcaatgttt ttacgatgat ttttccaatc
      481 tcttcctgtt cgcacgttgt caggatctca ttatacagca tgataagtac gctaaatgcg
      541 ttgttggctt caggaagcga tgagtagaac caaaaatcgg aaatcaaaat ctcacgaagc
      601 tgttctacaa caaaaagacg attgctgaaa gggtgtaaat actctataat acactcctgt
      661 gcacgcttat taatgccata atattttgcg gacagggcaa taaagtcctg caaatgcttt
      721 ggtataacta tatccttata gcgggtttcg gcaagattag cttcaagcgc tttggaatag
      781 aatttttcgc tcgtcatgtt atcaaaattt tgtcttcaaa gttagtgtga aatttggagt
      841 agcctctttt ggctaaatga attttgccaa tctgtatgag atggcaaagg gtgaattgcc
      901 ctgaaaagga ttttttttta aatatctgat ggaaaagtcg ctattagcag tccattttgg
      961 ataagtgata acattattga taattgcccg cccagttggt aatttccata atttttccgt
     1021 tttaattcat cttgtgtatt aatatataaa tctgcaaaat gttgttttgt tgaaataaaa
     1081 gacaagcgag ctaactgatc tcaatcttaa agatgttggt aaacctctat taaaaaattc
     1141 ttaatttgat ttttaatagt tgccggttcg tcatcgaaat tgttgatgat taatgctatc
     1201 gccaactttt ttccggaggc gactgtgaga tatcctgcat aactttgtat tcttgccatt
     1261 gatccggatt tcgcataaaa gttactttct aacgatgagc cttttccaaa attcctcaat
     1321 gttcctgaaa cgcctgcagt tgctaatgat tcttgaaata tttcggaata atgactgtca
     1381 ttcatatatt tcagaacaaa aactatctga tctgctgtaa ctccattaga tcttgagctt
     1441 ccacttccat ccatcaagaa tataccctcg ctatgcattc ctttatcttt ccagaacgat
     1501 tttaaaattt tacctccctc tgttagcgat ggttttccgg cagtggcggc taaatgcatc
     1561 aatagcacgt cggcatatag attaacactc tttgagtttg ttttattaat aatgtctgcc
     1621 aaattcggcg attttatttc aagtaatgtt ctcttttgaa ttttcgaaga tgctgaaaaa
     1681 gaggagtggg tatttccggt aattgctatg ccattcgttt ttaatttttc ggcaagctgc
     1741 tctgttacta attttgccgg atcaggaata gctcctttga taataaaatt ctcttttccg
     1801 gctggtatgg cgccggttat cacccgttta ttatccagat acgaaccaaa tatccaggca
     1861 ttatcacgat tatctttagc agagattacg tgattttcaa acgctaaacc ggtaatttca
     1921 ggagtaattc tgaggatggt tgtaggggtt cctggctttt ttttcgacct gaacgctatt
     1981 tcataactgt tgtcataaat atttaatcct gatgggatag ctccaaaata attaccaata
     2041 tcttcccaaa tccatgtacc gggaatattt ggttctccga atattgaggc atccccaata
     2101 atatttccat tgatcttttt tattccggca gcttttgttg catcgctaag tctgttgatc
     2161 acatttccat agttatcgcc aaaatagggg cttccaaagg tcggatcgcc tccgccaata
     2221 atgtataaat caccatgcaa tacgtcccct ttgtcaattt cacccgaata cgaaaggata
     2281 gtagaaaagg tatgataagg agtaaaggtt tccaaagcgg ctgccgttat aaatattttt
     2341 tgtactgaag ctggcacaac cgttaaagat tcattatgtg aaactaatac ttcatttgat
     2401 tgcaggtcaa ttatttttat agcaacggta gagtgagcca atgatttgga tgacaaaaac
     2461 ttctcaagaa ggtttttagt gctatcgggt tgctgtgctg acattttact tccggcagat
     2521 gtacagccgc cattaataaa gatactcaaa cacagggcaa tataaagttt aaaataagag
     2581 cgcataattt gtttaagagg gattgaagtg tgttaatctg tttccaaact ggcaattttg
     2641 gcttttttga tagcaaggat ctcgcttgca atttcttcta ttggtttgtt cgttactgta
     2701 attaccggcc ataagggttg tttactgaag ataaatcttg cgtaattaag ttccatattt
     2761 acatgttgta atgttgcata attggcagcg cccgagccaa gatgttcaag tcttacctga
     2821 cgaagtgtag agaggttata ggcataagct gttaggcaaa aaacgcgttc aggaggtatt
     2881 tgaaaaattt cattaggagg gggttgattt agtacgatag gcacgttagc caccatccag
     2941 cctttaaagg caaaatagat gcttagtggc gttttaaaag tcctggaaac gccgagcagc
     3001 acaatttcag ctttatgcaa ctcttcagga tgctggccat catcgtgatg aaaagcaaac
     3061 tgcatagagt caattcgttt gaaatactct ttattaattt cactgaaaag ccctggcttt
     3121 tcggtggggg agtgcattag tttttctgag agcctcgcta acaaaggacc cattaaatca
     3181 atagtttcaa cattataggt tctgcctgta gtgataataa aatcacgtaa ggaattagaa
     3241 accaatgtat gaacaataaa tgctttagtc tcggaagcct ctttcaccgc ttcctcaatc
     3301 tgttctttcg agcgaacatc gtttcgtatt atggtttcta aattatggcc cggaaactgg
     3361 gtaatggcag cattaagagc ttgagttgcc gtgcgaccgg taccgtcaga aacaataaaa
     3421 atcttaggca taattactct tttttaaact catcaaaatt aagaattttt acctcccact
     3481 tttctttgac atttagtttt cgggtaatga atggcttaat tgcaaagcca gttgcaatca
     3541 tcgaaccgct aataagcagt gttgacttgt caattattgg agattcattg ttgatcaacg
     3601 cattggcgcc ggcaataaaa aaataagcag cccctccctg aataaaaaaa agattagaaa
     3661 acccgcgtat taatccattc ggtctgaaaa taacaccaat atcgtttaac ccaatttccg
     3721 aagttttatt gataacaagg cttgtatcgt tgatctgtgt tatttttccc gacaacttaa
     3781 attcgcctct ttttgtttca aggtaaatgt aatcaccact gatgtattta aagtttttga
     3841 actttccttt tttctgcacg acaaagtatt gctgtgcgga tgcaactgcc gttagttgaa
     3901 atagtatgga taatatcatt attttcacaa gaagtctttt catgccggat gattataaaa
     3961 gtattattca gtgcgtgtca actgcatatt atagataata agagggacaa gatatgaact
     4021 tcactacgtt ggacgccttc aggtgtgatg ttttcggcta atactccaaa actcaccttg
     4081 ctccccgact ttttactcca tttttttaca ctctccacag ctaaaagact taattgcctg
     4141 ttacaatcaa ctacgtgagc atcacccact actctatact cgttgaggat aatctctgat
     4201 accgtcgttt taactgtacc ttttttttct gatatgttta cactaacaat attagccatt
     4261 tactatcagc tttattaaag aagcaaagat aatcaatgcc gaaattgatg ttttgcgtaa
     4321 ttactatttt tagcaaaaaa ttagaataat ggatggtata aaagaaatca tcaatcatat
     4381 agttgctttc agagacgaac gcaattggga acagtttcat aatgcaaaag accttgcagt
     4441 ggctctttct atcgaagcaa gtgagctaaa cgaattattt ctttggaaat cgcctgctga
     4501 aacaggcaat atagaaaggg aatttgtagc cgatgaactt gccgatgtgt tgatgtatgc
     4561 attgttgttg gctgacagat tcaagtttga tgtcaaagag attattttgg ataaaattaa
     4621 aaaaaacgct ctcaaatatc ctgttgaaaa gtcgaagaat cttgcattaa aatatacaca
     4681 attgtaatgc tcctgctctc ttttttactt agtggcaatc atgttgtaat atgatactaa
     4741 ggatctgaga tgttgtgggt ttcgcacctt caatttttgt tctttcataa actttttcaa
     4801 ttcattgcca tgcttgccaa atgtttcgca aaacgctgcc gagctagttt taacttcacc
     4861 tatttcgcct ccatcaaact gcaaaaagta tctttgtgtc cggtaaattg tctcttcgcc
     4921 tgttagctca tctacttgtt tatagctggc tatccatctg cgtaaaagct tgcaactccc
     4981 atcttccaat aattcaaggt agccagaacg acgtactccg tccgtaataa actcattgta
     5041 aataaatacc ttatcgttaa ttctgattaa gctaacctct tgcggatttg aaatggcttg
     5101 tatttcagta tcccgtacaa attgaatctg ttgtgtgagg atattataac gcatctgggg
     5161 caattcaatc tcactttgat ctttcataat tacaaggccg gagttccagt taacatctaa
     5221 ataggcatcc cctggttccc ccggtaaaac cgttggatag taggttgctc ccgaactttc
     5281 tgggagattg ggtaccacaa cgggttgaat atctattaca agatcctttg aaagaggcgt
     5341 tccttttttg attggatttt ggccatggag taaatgtaag ctttgtatta atagtagaac
     5401 taaaaacagt atcccgaatt ttttgattag tgattttgtt ttcataatgc atcattttta
     5461 aaacttctat ttagaaatcg gcccaaagat agtataaaat ttaggtgaaa gcaagagcat
     5521 aggtcagaaa aaagataatt cttaccataa aaatttaata attctttgcc attgttttat
     5581 ctctttcatt acggcgtagg tatagaccac ttttaaacaa gttttaagat tttaataata
     5641 atggcttatt cgccaatcta ttgatctctc attttgtaca tttgtatttt taaaaatgaa
     5701 aattgtagtg tctgaatctg taaaatatca agaattcatc aatcagacgg cctattatgt
     5761 taaagatcgc cttcgggggt gtgagggtgg gcatgattgg tggcatgttt accgtgtttg
     5821 gagaatggct aaacatattg ctgttaaaga aaaggtagat attctggtgg ttgagctggc
     5881 agctttatta catgatatag ccgattcaaa atttcacaat aatgatgagg agatcgggcc
     5941 gtatctttcc gagcaatttc ttttgaaaat aggggtggaa aaagcaacta tcgaacacgt
     6001 tgtcagcata atcagaaata tatcctttaa aaacagcttt gataagcctg ctttttattc
     6061 taaggagctg gcggtagtgc aagatgccga tcggttggat gcaattggtg caataggcat
     6121 tgcacgttca tttaactttg gaggatttaa aaacaggcca ctatacgatc ccgactctcc
     6181 tccaaaaaca aagatgacaa aagaggacta tcaaaagagt gattctccaa cattaaatca
     6241 tttttatgaa aagttgctgc tacttaaaga tatgatgaat accacaaccg gtaaaaagat
     6301 agccaaagat cgtcatcgct accttgtaaa gtatttagaa agatttataa aggagtggga
     6361 ggggaaattg tgatttttaa gattattgaa cccgaattaa tcatcagaaa gcgcttttag
     6421 ggggcatcat tgtttttata aggatagtgc caatttcgca ttttcttcag taaggttctg
     6481 attgtattga ttataaacgc ttaattgttt aagctttgcc aaatacttga tccgggatta
     6541 atattctatc cctttgcgcg ctgactggcc gttattataa taatgtttga tctctttcat
     6601 ttcagttaca agatcggctt cagagataag tcgttccgaa gcatatcgcc ctgtgataat
     6661 catttcaatg ctttccggct ttgatcttat cagcttaata atttcttctt cgtcgaataa
     6721 tttataatat agagctatgc aaagttcgtc aaggattacc acgtcatatt ctttggaagt
     6781 agtgattgtt ttaatttctt ctaatccttt cagagctgct gcaatatctt tgtctgttgg
     6841 ttgatttttt ataaaacagt caagaccgta ttgtcggata gttatgtttg ggatcagttt
     6901 taaagcattt agttcattgt agtgcatccc ctttacaaac tggccaataa aaactctttt
     6961 tcctgcgccg gcagctctta cagctaatcc aatcgcggcc gtagtcttac ctttgccgtt
     7021 accggtataa atatgtattt tgcctttgct cattagccta aaatattatt ataaatagtt
     7081 attattgctt ttgctgctaa agattcagga cgatattcca tgatggtctt acattgaacc
     7141 atagcctcta ccaccgattt gtcaaatgga ataagacctg caatggcgat gttgttttct
     7201 ttacaatatt gtattatgtg ctctgtcata tccgggttta tgtcagcttt attaattgtt
     7261 acccatattt tcaagttaaa tttattgcag atatctattg ttcgttttaa gtcgtgtagc
     7321 gccgatgcgc ttggctcggt aacaataagt gtatgatcag ttcctgttat ggtggacacg
     7381 acagcacatc cgattcccgg aggtccgtca attattatgt tttctatatt atgctttttt
     7441 gctatttcac gtgcctttgc acgaacgata ttaactaact tgccggaatt ttcttcgccc
     7501 ggagccaaac gtccatatac catttttcca tagcggaagc tgcctgaata cattcggctt
     7561 ttatcatttt gaactatcga gattgcttcg aacgggcata tccgtgagca taacttgcat
     7621 ccatcacata cctcttctaa aatatggaca atattattta cacatttaat agcatcgaaa
     7681 cggcaatacg aaatacataa accgcaatta ttgcatctgt cgtaatctat tactgccttt
     7741 tctcctccaa tataaacttg ttcctcctcg tgtgtgggat taaacagtat gaacatatta
     7801 gcggcatcaa catcgcaatc ggctagcatg accggctgtt gcatacttgt aaaagcagcg
     7861 ctgatactac tttttcctgt tcctccctta ccgctaatta ttgctagttc catatttttt
     7921 atgtatattg ttaagcaaat tatttaagtc ggattgtaac ttgggattgt actctgttaa
     7981 taattcccct tttgaatagt gataggctat ttctttatcg aaaggaattt ccattagtag
     8041 ttcgatattt tcatttttta ggtagtcgta aatatttgaa tcacctatgc ccgctttatt
     8101 gataatgaca ccataagctt tgccaaccgt ttttaatgtt tcgaccgatt gttttaagtc
     8161 gctcaggcca aagggtgtag gttcagtgat caaaatcaca taatcagcat ctaatattgt
     8221 gtgaatgaaa gggcaggaag tgccaggcgg tgaatcaagc agtacaaaag gttcgtttct
     8281 gatttgttta aggccggcct tgattaatgg cacaggtgtc ataactccaa cttttatgcg
     8341 cgactcaact attcgtgcat taggacgaat ctggtagttg cgtacaactc ctaaagagag
     8401 aggccgctct gttatcgcac cgaacttgca tgctacgttg caagcgccac agccatgaca
     8461 tagctcatca ataacatgaa tcatacttgc aggcggcaaa taaaatatgg cattatagtt
     8521 acaatattca tggcattttc cgcaaaaagt acatttgtcg gtgtcaataa ttggtgtttg
     8581 ttggagtact tcggcttcat ctatcaattt gccgtcaaaa aaaatcatgt cgttgggctc
     8641 ttcagcgtca caatcaacta aaacaacctg ctcgccactt tgcataaggt tgtcgaatag
     8701 gtgaacggag agtaatgttt tccctgttcc tcctttacca ctggcaatcg caactttcat
     8761 aaatattatt tttaattcac ggttaagtca ttttcgcatg aagctgtttt taaatgacct
     8821 ttttatggcg ctccttatta ttagttgctt tagcaaaaga tagaaagaca tgatttttct
     8881 tgtttgctta ggatagcttg tagtctatgt aaaacgtgat tatgtgtttt gtgggttaca
     8941 taattttagc ttatgattta caagcatggc taaaatgcct tttgtaaatt atttttgggg
     9001 tttacatgcg aacagactca aaatcagtgt tgcacttgct aactgcaatt tccgtgaggc
     9061 ttgtgttctt tgtgatcgca ataattagca gttaattcta acttgtttgc cagaaaatct
     9121 tcaacaagct cacgcgcggt ttttacaggc gctcctacaa atgtattaat attttgctcg
     9181 ttgaacagat gaatggcttt ttgtcccatt cctcctgcaa taacgtctgt tactccaaat
     9241 ttggccaccc atcgtggata tagacccgga acatgttctg ggggggtagt ttccacaata
     9301 tcggtaattt gattattgac tacttcgact atggcgaatt tctgacagtg cccaaaatgt
     9361 tggcttagta ctccattttc taatggaatt gcaatttttc tattcattta tttaaagttt
     9421 gctttggttt tttttaattc tagtctggat atttatcctt caagttgaat tgtaaatagg
     9481 attaaattac agatcaattc tattattctc ctccacgaaa tcggcgctgc attcctgttc
     9541 ttctgctact acagcgtcca aaccccatac ctcttctatg gcggtttcct cttggtgtaa
     9601 attgctcatc agggagattt tctgagcatc ttcccatttt tcttccggtc atggggcctt
     9661 tacccattgg tccagtttta tctaaattag gcatgatttc ctccttgttt ttttaaagat
     9721 tttgcatttc aataatttct tcgattgttt tattctcttt tataacaacc atttgaatat
     9781 tcaaactgtc gaaaagcgat ttgacttttc ctccaaattc tcctgaaaat actttagaaa
     9841 ctttgtgttt cgcaactaat tgaaccgatg atggtccggc gccttcattt acattgagat
     9901 ttggattcgg tataaattcc gtaacttgtg atactgtgtc gtagattacg aaataagcac
     9961 aacgtccaaa tcttgagtca acgtttgatt tcagattatt acctgtagat gtgattgcta
    10021 ttttcatata aaaaccataa agtagcttat ggaaaattct ccataagcta cgggttatca
    10081 acttaaatta ttaaataact atttaattcc tttaaatacc tcttcctttg ataaaggctt
    10141 tgtgcagaaa taccagtcgt aacatttcat atcaccctct ttgccttcaa cacgcgcttg
    10201 tgctgttccg cacgacccgg cagttggtac aattacatca tcgcctgggt gccaatcagc
    10261 aggagtagca acgttaaact tatcggctgt ttgaagcccg attaatacac gttttaactc
    10321 tttaaagttg cgtccaagtg aaagtggata ataaatgata gcccttatct taccaaccgg
    10381 atcaatgaag aatactgctc ttactgcttt tgtgttgctt tctccaggtt gaaccatgcc
    10441 atatttattt gcaacttcca tagtaatatc ttcgattaga gggaatttta cttcaatatt
    10501 tttcatccct ttaaactcga ttttttcttt gatggatctt agccatgcaa tatggctata
    10561 gagaccgtca attgacaaac ctatcagttc gcagttaagc tctttgaatt ctgcttccat
    10621 gttggcaaaa gtcatgaatt ctgatgtaca tacaggggtg aaatcggccg gatgactaaa
    10681 aagaattacc catttccctt tataatctga cgggaaattg acctctccct gtgttgttac
    10741 agctttaaat gctggtgcat tatcaccaat tcttggcata gcaaaaattt cttgttccat
    10801 ggtttttgat ttttaattgt gaataaaaga tttaaattaa ttgctgtttt gtttacagtt
    10861 ttttaaaatt tcatttatag taatttgatt ttcaggtatg atcatttgta tgtcttttgc
    10921 tcttaacgtt tcaatagctc tcgaaccaaa tcttcctgca ataacaacgt tgatgctttt
    10981 gccaaacagt aaatcaatgg ctttatatcc tgcaccttta gtcaaattaa acgcagtgtt
    11041 ggggataaac tcataattat tggtctgggt atcgaaaata caataccatt cacatctgcc
    11101 aaaatgccgg tcaacaaatg cgtctatatg attgtcggta gctgctattg ctattttcat
    11161 tgtattaatg actttcagtt attaattcgt ttttgcaaag tggacaagcc atagggctat
    11221 ccttttgtaa tgtaaagctg attttgcatg tatcgcattt gtaccagctt ataatttcaa
    11281 cattcccccc tataaaacgt acatcgtgcc cctcgataaa ggctttggca atttttcggc
    11341 gagcgctttc gtaaatcctg gtgaaggtag ggcgggaaac cttcatcatt cgggctgcct
    11401 cttcctgtgt taataactca taatcacaca gatttatcgc ttcatattcc tcgtaattaa
    11461 ggaaaacatc agtatcatgt ttccacttta atcctcttgt agtgaagcca tgaaaatgtg
    11521 gagctcgttg aataaatcta tttttcttac atcgtcccat caaattaact ttagtgcaaa
    11581 gataatgaac atatgttcat aataaacaaa ctttttatag aaaaagttca attatgtatt
    11641 taagtgatta agaatcaata taataaatat tattttttct taactaatag agctaccgaa
    11701 agtgaattgt tttaaaatta gtatttctaa ccttttaaaa gataaaacat taattttagc
    11761 ccaaatagtt gttattgtgg aacgatttgt aatttgatag caacaaatgt ttaattaaaa
    11821 acgcttctat ctttacatac tcgttgttgg aatggaaaat tgattagcaa aatccgatta
    11881 atcttattta agttttgatc ttaaacaatt gttttcgtga gtttaaaaaa atgtttcacc
    11941 ctgtaatttc tattgtttat aattcttggt ttattaaaag aggtggcttt aagctgcaaa
    12001 tgcaactttt tggttaaaca ataatttttt caacatttac tgggggttaa aatccccaaa
    12061 ctctttcttg gtcaatactc cattaaattt gtataggatt atgaaaaaca acctcttctt
    12121 tttgattaat cttttaacaa aagaaatgaa tcatctctta agattaatca tcttttctca
    12181 agttgttaat attttgagtc tttgttactt atactttttt taattttaaa ttccctgttt
    12241 ttacttttta ttttgattta tccgggaatg gaggagctat cggatattgc gggtcagggt
    12301 agtctttaag taactccaaa attacctcaa tagctttatt aagctgctga tcaactcctg
    12361 agaattcaag cgctggatcg ttccatattt cgatgtcagg ttctactcct gttccttcga
    12421 ttatccattc tttgccatct gcgctatagt gtgcgaattc aggtttcata agcgaaccgc
    12481 cgtcaataaa tggcaatgat cctctgatcc cgacaacgcc gccccaggtt cgtgttccaa
    12541 ttagcggccc gagattgtgt ttacggaatt gatatgggaa gaggtcgcca tctgatgccg
    12601 aatatcggtt tataagacat accaacggtc cggccatcat tccgtcaggc ttagaataac
    12661 ttacgctatt gcggccagca gccatcatcg aaagctccct gcgtaagcgc tcaatgatca
    12721 ttggtgatac attaccgcca ccattacccc gatcgtcaat gattagagct tttttgcgaa
    12781 gctgtggata ataatatctg acaaattggt taagaccgcc aggccccatg tcgggaatat
    12841 ggatgtagcc cacctctccg ttagttgctt tattaacctt gtcaatatta tcgtgtaccc
    12901 aattgtagta atagagttca ctttcgtttg ctatcggctg aatgagcacc ttgcgtgcgc
    12961 ctgattccga tggaaggctg ttaactgaaa tctcaacagt ggttccggat tttccaacca
    13021 acgatgcgta aatgtcgttc atctctttgg tcgtctgtcc gttaacagca ataataaagt
    13081 cgccctcttt tattccgagt ccgggctcca tcagcggcga acgtatattt ttattccagt
    13141 tttgcccctt gagtatttta tcaatttgat aaaaatctga tttgtggtga ctcaattcag
    13201 ctccaagaag tcctgtagca atgcgctcag gttttattag gtctccgtca ttaacgtatg
    13261 catgaccaac atttagctcg ccaatcatct cgccgattac ataactgaga tcatatctgc
    13321 tatttacgtg cgggagcaat agattatatt tttcgtgcat tttgtcccaa ttcacgccat
    13381 gcatgccagg gtcatagaaa aagtatttca tctgccgcca cgattcatca taaatctgtt
    13441 gccactcagc tttaggatca acccaaatct tcattcctga aagatcaact gcttcgtcaa
    13501 tttttactgc cgctttaggt aagtcagtaa ctgtatattt tcccattgag ttaatcagca
    13561 tttttttgcc attgcccgaa attgtaaaat tcatattaga accaatctct gtctcttttt
    13621 gatcctcaaa cttaaaaaat atcaacttcg agctattact gcccattttt gattggatgt
    13681 aataaatacc tccttctaca cctttaatgt tccagtagta gcctgactct ggtgtaatct
    13741 caacaacgcg atcgtaaata ccatcaaaat ctatgtttac atttttctga tcgttctcaa
    13801 cagctttttc ttctttttta tcttttgccg gttgttcatc tttttttact tccggtgtat
    13861 catcttttgg ctcaaaaggc gaggtggtat ttttatctaa agttactaaa tacaccttta
    13921 ccatatcagc ataaacatga ttccactccg tccagctata aattggatta aagctacgca
    13981 ttgagctgaa gaataggtat ttgccgcaag ggctaaaact tggcgagctg gaattgaacc
    14041 atgagtcggt tattggagtt gatgttttgg tttcgaggtt gtaagcatat attctcgata
    14101 cctcgtttgg tattgtgaaa acgtatgaga gccagcgact gtcgggcgac cagtcgtagt
    14161 tgctgatttc attgctttcg gaggaggcta caagagtagt ttcttttgaa ctaatatcaa
    14221 caaatagcaa acgcttcaac tgatcgtgcc atgcaatctt tttactatca ggcgaccagt
    14281 ttatcttaaa cttataagta tcagcatttt ttgtaagttg gattggcttt tctatgccgt
    14341 ccggtttcat aatatatatt tcatcttcgc ctgatttgtc ggaaataaat gcaatccagt
    14401 ttccgtctgg cgaccattcc actgctctat cgtgctctcc gtttgaatct gttaaatttt
    14461 ttgtaattcc tttttttgcc ggtacattga atatgtcgcc acggctcgag aaggcaatgc
    14521 ggcttccgtc atatgagagc gaagctgccc gaattgtttt atcagcctgt ttccattgcg
    14581 gacgacttgc ctgaaaatcg ttcaggattt gtatctcaat tttcactggt tgttgtgtag
    14641 taagatcgaa ataatagaga aagccgccat tttcatatac tatggcatta tcgccaaggg
    14701 atggaaactt gatgtcgtaa tcgctgtagt ttgtcagttt tgtaacctgt tgtgttttgg
    14761 tattgtatgc aaacaagttc attgtccggt cgcggtcgga taagaagtaa attgaattgc
    14821 catgccacat ggggattata tcttgtgcct tgttattggt gatgttgaca gtatttttag
    14881 tttcaaaatc atatatccaa acatcgtctg ccattcctcc atggtaatat ttccatgtgc
    14941 gaaactccct aaatacttga ttgtaagcca tttttttgcc atctgccgag aaactgcaaa
    15001 aaccaccttc aggtacctct acttgtttgg aaagtccacc attaatggat acttcgaaaa
    15061 ggctacctat aaagtcgtta aagcttttct ttcgcgatcg gtaaatgatt gtgttattgt
    15121 ctttccacgt catcactata ttatttggtc ccattcggtc gcttacatca tcacgttgta
    15181 atgtagcggt ataagtaact cttctgggaa cgccaccctc tgctggtatt acaaatactt
    15241 cggtattgcc gtcgtattgc ccggtaaatg caatatattt tccatccggc gaaaagcgtg
    15301 ggaaaagttc gttgccttta tcgttggtaa gttggcgagc tatgcctcca gatttttgaa
    15361 ctgtgtaaag gtcaccggca taactgaata ccacatgatt accatgaata gtgggaaaac
    15421 gcataagtcg agtttcatgc tgggctgtaa ctgttagtgc agccattgaa aaaaagataa
    15481 cggtaaaaaa aagttttttc attggtaaaa aatttgagaa atagattttt ataaaatgaa
    15541 agctgttatt tcaatatttg acatggataa tggtccgaca attccttttt ttgcattaat
    15601 tacggataga tcatacaaaa ttctgtaaat ctgacatttt ttatgaatag cttttttcga
    15661 aatatccttg tgaggtgaac gagaaattaa gaaaaggaca tggcgcttat caagcatcac
    15721 ttcagctaac gctttgccta accatttcaa agcgcctatg atgataaaat accttttcaa
    15781 tttgtttttt ttaatgttaa ttaatttaaa atgataagtt tattgcttaa gtatccaggc
    15841 ggctttgtag cgctcaccta atttattttt agcttcaagg gcttgttctc tggtggaata
    15901 attgcctaaa gaaattctgt aatttgtttc ggaagcaacg actgatgggt ttttaaagcc
    15961 ttttgctttt aaggtttcta cctccttttt agcctggttt ttatctttta cacttgaaat
    16021 gataagataa tatctcacct cttgtccggc ttttgtggat gttttttcct gttcatttgt
    16081 tggtagaata cgttcgagca gggcttttgg ctctttggct ttgcatacat taacactttt
    16141 atcaggatca ccctccacgc caaaccaggc aactgcatat cctttataaa tccctattcc
    16201 caaagacttc cagttgtaat ttttccaagc cccctggttg aggatcatct cttttgaaac
    16261 aatagaactt agccaactgt caaggacgat ttcgggtact gcttcaaggc tttcccaaaa
    16321 agccaactcg tatcctttcc cattgtaagt tgttagctca gcaggcttgg aggtcataca
    16381 agtattgttc aacgattcgc taccataaca gcactcagtc caaataccct gatccgacca
    16441 ggaatgtaga ttacaattac ctacatcggg cctgttggtt tgtagatcgt tgacatgcaa
    16501 tttggcaaca aaacataaat ttttagaaag tgaaacctcg ggtaaattat actccttacg
    16561 gtaattgtta atcaggttga ataattttaa ctcgttagaa gtaatacaaa aatcatcggg
    16621 gattttgatc tgagctatca atcgaggccc aaaaaagcta attatcaata tcaaaaccgt
    16681 aatattcctt aaattcaatc ttttagagag cataaacatt taattttcag ggtatctcta
    16741 tgaaacttca aaagtagaat tttttttaat tataaaggat aactatgtgt gttgattgag
    16801 attttatttg actgattttg ggaaaacata ccttgcgaag gataattaat ctttctgaca
    16861 ataatccatc tatgaacagt ctgttattaa ggctgaaact aattataaag gagttaatgg
    16921 atgatggaac gaatggttaa atgtttactt tgaagtattc gaaaccatga accaagcttg
    16981 tcgtcaataa agggaaatca aatcttagaa aaactgaagg agagtcgaat agctgataaa
    17041 acgtcagaaa agcgatttgc gaataagaga gaattaatcg tatagttgta aaaattcata
    17101 actatttcct gtccagtcaa ggtaacgaat aaaatcttta aaggcgatga ttactgaata
    17161 tgtattgatg tttggatgga aacttatttt tgaagctgtt tttaggtttt catctaaaaa
    17221 tatatggaca tggttatctt tgtcattaat cagtccaaat ggggaaacag atcctggtga
    17281 tattcccaaa tatttttgca ttctttcggg cgaagcaaat gaaagttttc cttgtcgaag
    17341 tcgtttttca agatcatgaa tagcaagatt tgctgtatgc tcaataataa caaggtagtg
    17401 tctgttacct ttatgatttc tgaaaaaaat gttcttacaa tgtgttgctt cgataaactt
    17461 ccaatgcttt tttgcttctt caatagtagg agcaggcggg tgttcataaa tttcataggc
    17521 tatagccaaa cctttaagga cactgataac catttccttt ttcttttcca ttactttttg
    17581 attaatttaa ttaatttata agaaaatctt tctttttaga tttatttaaa aaataaaatc
    17641 tatttttaca ccccaaaaat attgaaaatt tcaaaatctt gaattattaa cttttattat
    17701 taattaaaac aattaggaca atgagattat ttctaaactt taaaaagcaa tttagttttg
    17761 taatgcttat tgctttagta ttttcgtttt ttggcgctaa tgcccaaatt catag
//
LOCUS       DAOOAI010000044        17107 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_4355_length_17107_cov_5.351806, whole genome shotgun sequence.
ACCESSION   DAOOAI010000044 DAOOAI010000000
VERSION     DAOOAI010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 17107)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17107)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17107
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4355_length_17107_cov_5.351806"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1260)
                     /locus_tag="PK345_04585"
     CDS             complement(<1..1260)
                     /locus_tag="PK345_04585"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPX34330.1"
                     /translation="MLLERFTVFEIATIVKGKLLNGQLSGLSVNEILVDSRSLFRGEG
                     TAFFAISTRRNDGHRFIGELYEKGVRCFIISNPEFDIKPFPKAVFVLVKSSLQALQEI
                     AAAHREKFNIPVIGITGSNGKTVIKEWLYQLLSTDKRVIRSPKSYNSQIGVPLSVWQL
                     EPEYELAIFEAGISEPMEMQRLQQIIKPTIGIFTNIGEAHSANFIDKRQKITEKLSLF
                     KEAETLIYCTDHKELHEVVITSDLFKNIKFLTWGWEGSNDLQITDVKICDKGKTKIDA
                     VFRNKNISIIIPFIDNASVENATHCWATLLLLEVDQDVIAEKMLSLTPIAMRLELIEG
                     INDCTIINDSYNSDLLSLGIALDFLNQHTNHKDKTVILSDMLQSGQSEFFLYRKVAEM
                     LKQKQIKRLIGIGPTISRQSEQFEMQSE"
     gene            complement(1318..2418)
                     /gene="pdxA"
                     /locus_tag="PK345_04590"
     CDS             complement(1318..2418)
                     /gene="pdxA"
                     /locus_tag="PK345_04590"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HPX34331.1"
                     /translation="MEDTQVKLPELPPVRIGFTHGDVNGIGYEIIIKTLADDRINEMI
                     VPIVFGHSKVASYHRKTLTVKDFSFNLIKRVDAANPRRANIYNITEREVKLDLGAMTK
                     IGGEMAYCSLKSAVESIDKKEIDALVTAPINKKNIQSKEFNFPGHTEFFASRYNLSNY
                     LMMMISGPLRIGVVTGHIPLKDVAQTLTKELILDKINVLNQSLLQDFGIRKPQIAVLS
                     LNPHAGDNGLIGTEEQEIIIPAIKEAFESKILVFGPYPADGFFGTTNYRSFDGILTMY
                     HDQCMLPFKTLAFADGVNYTAGLPIVRTSPSHGTAYDIAGKNIADENSMRAAVYLAVD
                     IVRNRLQYSEITANPLQKTELVDEVDEDIQLE"
     gene            complement(2598..2720)
                     /locus_tag="PK345_04595"
     CDS             complement(2598..2720)
                     /locus_tag="PK345_04595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34332.1"
                     /translation="MKYVTEAVLRQVQQPSKVTWLLGEVEAQQLGACEAPPMVL"
     gene            complement(2892..4070)
                     /gene="ricT"
                     /locus_tag="PK345_04600"
     CDS             complement(2892..4070)
                     /gene="ricT"
                     /locus_tag="PK345_04600"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HPX34333.1"
                     /translation="MENQNIKSDNDIDKKFLTRGCSQAPQLYQDNKNIFVGRCCKLNA
                     HDWLQNVQLPEDHSLFNCVEVRFKNCRKDFFRIHGDMKFTTGDIVAVEATPGHDIGIV
                     SLTGEIVKLQMLKKGVDPYSPQVKRIYRKARLADIEKWVNTVDLENEATLKSRKMAWD
                     LNLDMKINDVEYQGDETKAVFYYTADERVDFRQLIKVLADHFKVRIEMRQIGVRQEAA
                     RLGGIGSCGRELCCSSWLSDFTSVSTSKARTQLLPMNPQKLAGQCGKLKCCLNFENDV
                     YLDALKEFPDNSIKLKTEKGEAIHQKSDVFRKIMWYSYLDDQSKMLALTIENVKQILE
                     QNLKGVYPKSLEDHALQQEAKVEEAATQLDEGDIHRFDIKAGKTSKKKRRKNNNSQND
                     "
     gene            complement(4205..5344)
                     /locus_tag="PK345_04605"
     CDS             complement(4205..5344)
                     /locus_tag="PK345_04605"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HPX34334.1"
                     /translation="MQFNKIIGQDHLKKKLRETVKNNRVAHAQLFYGGEGVGKLPLAI
                     AYAQFINCKDENKFINGDSCGTCPSCNKYNKLIHPDLHFVFPIAGGDESKKPISSNFL
                     TEWRELLLQNDGYITLNDWYQKIEIERKQAVINVSECNQIIQTLNYTSYESDYKVMII
                     WMVEKLYYVAAPKLLKILEEPPDKTLFLLIAEHTEQIINTIMSRLLPVKIPRIDDDAI
                     TEAIKKRFNVDDPIANQVAQQANGSFTEAVSLLDQSEDRKANFDRFKDWMRMCWTFDA
                     QGLLDFSEKVAREGRESNKSFLQFSLVAIRNSLALNYTKYAYIRIAEEERVFYENFSP
                     FVNHLNIVMFTNEFNKAINHIERNVHSALVFFDLSLTIVKLLKMK"
     gene            5436..6053
                     /locus_tag="PK345_04610"
     CDS             5436..6053
                     /locus_tag="PK345_04610"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPX34335.1"
                     /translation="MLLKIHPVTPSPRQIGKVVECLYDGGIIIYPTDTVYGLGCDIFK
                     SKAIERIARIKGLKPEKANFSFICHDMSQITDFTRPISNNLFKLIKRVLPGPYTFIFD
                     ANNTVPKYIQSRKKTVGIRIPNHNIPLEIVRQLGHPIMTTSIHDEDELIEYTTDPELI
                     YEKYKDLVDIVIDAGFGGNVPSTVIDCTGDEIEVVREGLGPLTIF"
     gene            6168..6240
                     /locus_tag="PK345_04615"
     tRNA            6168..6240
                     /locus_tag="PK345_04615"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6201..6203,aa:Lys,seq:ttt)
     gene            complement(6302..7375)
                     /locus_tag="PK345_04620"
     CDS             complement(6302..7375)
                     /locus_tag="PK345_04620"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34336.1"
                     /translation="MSCTVYAPLIEEVYIRYGSGMGNLNVWGLSRYDSNFVIEEFKTQ
                     YGVSHPCAGSEGNAGEAIDVLIDGQIYYGTPTYLVICPDYKMHFDICFPPEMECIDSY
                     IQFCNMGLIADFSAEVNQVCQGSYIQFNNESYGNITNWDWQFEGGVPPTSNEMNPLIF
                     YPEPGLWDVTLTVSNTLFTDTTIETDFVEIYANPVVTLQPIDTVCEYDPPFRLIGGYP
                     LGGSYSGNGVQHGVFDPQAAGVGEHIITYTFEDENGCTGTDEQILTVDVCAGINKLKI
                     SYADVYPNPSKGELFIRTKDTDCIIVQIIDLVGSVIISKTFYQSVWDYVSIDLSRLPS
                     GFYMVIVNDGSTIYTTKISLLKE"
     gene            complement(7376..7594)
                     /locus_tag="PK345_04625"
     CDS             complement(7376..7594)
                     /locus_tag="PK345_04625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34337.1"
                     /translation="MVKKLFFAIALLMFMLSVSAQDRTFEVTSTDGLTELKVVGNFTI
                     TDSDGYNWTLYDELDKGNTIFIDIFQAT"
     gene            7721..8728
                     /locus_tag="PK345_04630"
     CDS             7721..8728
                     /locus_tag="PK345_04630"
                     /inference="COORDINATES: protein motif:HMM:NF014477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0017013 - protein flavinylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HPX34338.1"
                     /translation="MKNINLFTLILLSVIISCTSSHNNTKIKFIGETQGTYYAITYFA
                     SDTLIAQVQVDSLLADFDMIASIWEPNSLVSKINRNETDEANNLHFEEMFNISQKISA
                     ETEGSFDITVGPLVNAWGFGFKNKQDLTKEMVDSIMQFVGYRNVRLENGKIIKDHPNI
                     QIDLNAIAPGYAVDILGKYFESKGIKVYLIDIGGEIITKGLKPNGEKWIVGIEKPSEE
                     ATSGRILKAKVKVADIGLTTSGNYRKFYIKDGVKYSHTINPHTGYPVTHSLLSATVFA
                     SNAAFADGYATAFMVMGLEKTKLFLEKNKNLGVYLIYTDEAGNLRTFTTPDVEKMLNE
                     L"
     gene            complement(8715..8942)
                     /locus_tag="PK345_04635"
     CDS             complement(8715..8942)
                     /locus_tag="PK345_04635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34339.1"
                     /translation="MLKVFLISIVFVGLSFIGIAIKMLLKKGGEFQKSCGSVDPTTGK
                     RIACTCGKMDDERCNNKKRYEDIEFEEIKAH"
     gene            complement(8990..9775)
                     /locus_tag="PK345_04640"
     CDS             complement(8990..9775)
                     /locus_tag="PK345_04640"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HPX34340.1"
                     /translation="MLSAAQIKLIRSLHLQKFRKSANLFLVEGNKMVAELLRSDYGIE
                     AIYATEKWFVKNRGEFFKDKFEVIKISSAELKRISALKTPNDVLALVRIPEPSLNLEA
                     FNNLVLMLDGIADPGNLGTIIRIADWYGIKTVICSSDSVELFNPKVVQATMGSLFRVN
                     VFYGFLPEIFQKYCSDIPIYGATLDGVNIYAKELPEKAVIVIGGESHGISAEVKEKVD
                     FLITIPSFSSAAESLNASVATAVICSEFRRKALKASVLFEKFD"
     gene            9912..10409
                     /gene="tpx"
                     /locus_tag="PK345_04645"
     CDS             9912..10409
                     /gene="tpx"
                     /locus_tag="PK345_04645"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0016684 - oxidoreductase activity, acting
                     on peroxide as acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HPX34341.1"
                     /translation="MAKITLKGNPINTVGNLPVVGSIAPDFKLVKSDLSILSLEDLKG
                     KRVVLNIFPSLDTDVCATSVRRFNAEASKLDNTVVVCISKDLPFAHSRFCATEGLNNV
                     VTASDFRDGTFGISYGVTIADSPLEGLHSRAVVVLDINGKVKYTEQVPEIAQEPDYQA
                     ALAAL"
     gene            complement(10525..10971)
                     /gene="rplI"
                     /locus_tag="PK345_04650"
     CDS             complement(10525..10971)
                     /gene="rplI"
                     /locus_tag="PK345_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HPX34342.1"
                     /translation="MEVILKQDIENLGYENEIVKVKNGYARNYLIPKGMAILATDTNK
                     KILAEVQRQKHFKEQKLRKDAETTQKLIEKIELRIGAKAGTSGKIFGSVNAIQIAEAL
                     KEKGFDIDRKKVDVDGENIKELGNYIAKVHLYRDIKAELKFEVYAE"
     gene            complement(10984..11253)
                     /gene="rpsR"
                     /locus_tag="PK345_04655"
     CDS             complement(10984..11253)
                     /gene="rpsR"
                     /locus_tag="PK345_04655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HPX34343.1"
                     /translation="MAQQNSDIKFLTPIAVDVKKKKYCRFKKLGIKYIDYKDENFLLK
                     FVNEQGKILPRRITGTSTKYQKKVAQAIKRARHLALLPYVADLLK"
     gene            complement(11272..11637)
                     /gene="rpsF"
                     /locus_tag="PK345_04660"
     CDS             complement(11272..11637)
                     /gene="rpsF"
                     /locus_tag="PK345_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009437653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HPX34344.1"
                     /translation="MLNQYETVFILTPVLSDEQMKETVRKYQDLLKSKGAEIVYEEHW
                     GMRKLAYPIQKKSTGFYHLIEYKAEGELIFEIELAFKRDERVIRFLTVKLDKHAIAYN
                     EKKRAKAKDEVPVKEEQIN"
     gene            complement(11901..12836)
                     /locus_tag="PK345_04665"
     CDS             complement(11901..12836)
                     /locus_tag="PK345_04665"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPX34345.1"
                     /translation="MKLKPVYTYILITLSMIFWGGSFVLSKILLKYYDPITTVLLRLI
                     LSSLIMFGGLKLFNRLQKIKKEDYKLFLLSAFFNPFLYFLSENYGLKYSSPTVSSVII
                     GTIPLFTPIVGYIIFRERLTWLNIVGLLISFFGVIIMLIDKDFSLTASPVGVAWLFLA
                     VITAVLYAVFLKKLAFKYDAFNIIAVQNFIGIFYFLPLFFILDFRHFITVRPNAEMLV
                     SLLLLAFFASSLAFAFYTIGAREIGISKTSLFSNTIPVFTALFSFFVLKEVFEAEKII
                     GIALVLTGVLLSQIKGKRVLVNFYRFIFFGKKHDL"
     gene            13023..13787
                     /locus_tag="PK345_04670"
     CDS             13023..13787
                     /locus_tag="PK345_04670"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPX34346.1"
                     /translation="MQNLNKKLDSAAEVVSRSKYLVAFTGAGISVESGIPPFRGADGL
                     WSKYDPMVLELSYFYHNPLESWKVIKEIFYDFFGKAAPNKAHLALARLEQLGILKSVI
                     TQNIDNLHQLAGNTRIFEFHGNSQRLICTNCNEIHIPDKVLLENLPPLCASCNSLLKP
                     DFIFFGEQIPMDAYKYSIEAAEKCDVFLIIGSTGEVSPANQIPSIAKRKDATIIEINP
                     EESHYTSTLTDIYLCGRAVEIMQLLMARVEKLIERN"
     gene            13827..13899
                     /locus_tag="PK345_04675"
     tRNA            13827..13899
                     /locus_tag="PK345_04675"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:13860..13862,aa:Gly,seq:ccc)
     gene            complement(13972..14376)
                     /locus_tag="PK345_04680"
     CDS             complement(13972..14376)
                     /locus_tag="PK345_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34347.1"
                     /translation="MKKISFFILSFFLLLGVTSCVDDVFTDEDERAAFLGTWVVSETC
                     VRFNYEVEIKADTDSDTKVFLYNFALAGLEYSPAYGFVKGNVVEIPSQTIGDDWTVKG
                     SGYLKSAGKIVWNYEIVIAGDQSDCQADYDKP"
     gene            14827..16827
                     /locus_tag="PK345_04685"
     CDS             14827..16827
                     /locus_tag="PK345_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1,6-bisphosphatase"
                     /protein_id="HPX34348.1"
                     /translation="MRHQPIDTKFTHEEVEKEIRYLQLLSKQFPTVQTASTEIINLEA
                     VLQLPKGTEHFLSDIHGEHEIFSHVLRNGSGVIRQKIEDVFKNTLRKFIKDELATLIY
                     YPEEKLALIEKSESDIEEWYSVTLQRLVEVTRVAASKYTRSKVRKSLPTDFAYIIEEL
                     LHEKPSSPNKAKYFDEIIQTIIRIDRAKEFIIAICKLIQRLSIDRLHIVGDIFDRGPA
                     AEKVMEMLTNYHSVDIQWGNHDIIWMGSAAGIRAMIANLIRISARYMTLDTIENGYGI
                     SLLPLATFAMERYKNDPCNLFTPRIPEGAEASTKEIEMIKKMHKAIAIMQFKYEWEII
                     KRNPQFEMDNRNLLDKIDFANGTLEIDGKNYQMLDNNFPTVDPKNPFKLTEEEIEVME
                     KLRISFMHSELLKKHVKFLYSKGSMYLRYNDNLLLHGCVPMDNDGNFIKMILCEKELS
                     GKALLDQFDLFARKAYFTRNDKQIDTIAHDHTWYLWCGKYSPLFGKKKMTTFERYFIA
                     EKGTHIEEKNAFYKLNDNEDVIKKILAEFELNPEIAHLINGHVPVKVKAGESPVKANG
                     KLLVIDGGMSKAYQKVTGISGYTLIYNSYGLVLVAHQAFENTTKAIEEESDILSNITY
                     LASNPTRKTVADTDNGKILRQQITDLEMLLAAYRKGLIKEIR"
     gene            16923..>17107
                     /gene="rpsO"
                     /locus_tag="PK345_04690"
     CDS             16923..>17107
                     /gene="rpsO"
                     /locus_tag="PK345_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018357726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HPX34349.1"
                     /translation="MYLTKEQKASFFETYGKSKQNTGSAEGQIALFTYRIKHLTEHLK
                     EHKKDLNTRRSLVLLVG"
BASE COUNT         5508 a         3384 c         2947 g         5268 t
ORIGIN      
        1 ttccgattgc atttcaaatt gctccgactg ccggcttatg gtcggaccaa tgcctatcaa
       61 ccgcttgatc tgtttctgtt tcaacatctc tgcaactttc cggtaaagaa aaaactcact
      121 ttggccactt tgcaacatat ccgacaaaat cacggtttta tccttatgat ttgtatgttg
      181 atttaaaaag tcgagcgcaa tacccaatga aagcaagtcg gagttatagc tgtcatttat
      241 tattgtacaa tcgtttatgc cttcaatcaa ctccaatcgc attgctatcg gagtaaggct
      301 aagcatcttt tcagctataa catcctgatc aacctcaagc aacaagagtg tagcccaaca
      361 atgagttgca ttttccaccg aagcattatc aatgaaagga ataataattg aaatgttttt
      421 attcctaaaa acagcatcta tctttgtttt acccttgtcg catattttaa catcggttat
      481 ttgtaaatca ttacttcctt cccaccccca ggttaaaaat ttgatatttt taaataaatc
      541 agaagtaatg accacctcgt gtaactcctt atggtcagtg cagtaaatca aagtttccgc
      601 ctctttaaac agcgaaagtt tttcggtgat tttctggcgt ttatcaataa agttagcgct
      661 atgtgcctct ccgatatttg taaaaattcc aatcgtaggc ttgataattt gttgtaatct
      721 ttgcatctcc atcggttcgg aaatgccggc ttcgaatatt gccaactcat actccggttc
      781 caactgccaa acagataatg gcacaccgat ttgagagtta taacttttcg ggctacggat
      841 aactctttta tctgtagata ataactggta cagccactcc tttataacgg tcttgccatt
      901 gcttcccgtt attcctatta caggtatatt aaatttttca cgatgagctg ctgcaatttc
      961 ctgaagcgcc tgcaaagaac tttttactaa tacaaacaca gccttcggga aaggtttaat
     1021 gtcaaattcc ggattggaaa tgatgaaaca ccgcacgcct ttttcataaa gctctccgat
     1081 gaagcggtgt ccgtcgttgc ggcgggtcga aattgcaaaa aaagcagtcc cctcacccct
     1141 aaataaggag cggctgtcaa cgagtatttc attaacagat aaccctgata gttgaccgtt
     1201 gagcagtttg ccttttacaa tagtagcaat ttcaaaaacg gtgaatcttt ccagtagcat
     1261 taaatgatca gtttgaatag aaaattttgt tccttattga taatacgata taaacgccta
     1321 ttcaagctgg atatcttcat caacttcgtc cacaagttct gttttttgaa gcggatttgc
     1381 ggttatttcg ctatattgca gccggttgcg tacaatatcc acagcaagat atactgctgc
     1441 acgcatcgaa ttttcatcag caatattttt tcctgcaata tcatatgccg ttccatgtga
     1501 tggcgaggta cgcactattg gtaatccagc agtataattc actccatcag caaaagccaa
     1561 tgttttgaaa gggagcatgc attgatcatg atacattgtc aaaattccat caaatgaccg
     1621 gtagttggtc gttccaaaaa agccgtcggc cggatatggg ccaaaaacca aaattttact
     1681 ttcaaacgcc tccttaattg ccggaataat aatctcttgc tcttcagttc caatcagtcc
     1741 gttatcgcct gcatgaggat taagactgag tacagcaatt tgcggcttcc tgataccaaa
     1801 gtcctgaagc agagattgat ttaaaacatt aatcttatct aagattagct ctttagtaag
     1861 agtttgtgca acatctttca gaggtatatg ccctgttaca actccaattc ttaatggtcc
     1921 actaatcatc atcatcaggt aattggacaa attataccga cttgcaaaaa actcggtgtg
     1981 tccgggaaag ttaaattctt tcgattggat attttttttg ttgataggcg ctgttaccaa
     2041 agcatctatt tcttttttat caatagattc aacggctgat ttcaatgaac aataagccat
     2101 ttcaccgcca atttttgtca tcgctccgag gtcaagtttc acctcccgtt cagtaatatt
     2161 gtaaatattt gcccgtcggg ggtttgcggc atcaacacgc tttatcaggt taaaactaaa
     2221 atcttttact gtcaacgttt tgcgatgata agaggctact ttagaatggc caaaaactat
     2281 cggaacaatc atttcattta tccgatcgtc tgctaatgtt ttaatgatga tttcgtagcc
     2341 aatcccattt acatcaccat gagtaaaacc aatgcgtaca ggtggcaact caggcagttt
     2401 aacttgtgta tcttccattg attcagaatt tttacaaaga taggtgaaat catcaataag
     2461 gataaaaata gcagcaatct aattaaaaca tcatcataaa aaactctcga tagaaaatct
     2521 tggaacgaaa aattcccagg gagtttggat agcgtaaaaa cgaaaatgga gttttgtgtc
     2581 tcttcgcaaa acagacctca caaaaccatt ggcggcgcct cacaagcgcc caactgctgt
     2641 gcttcgactt cgcccagcaa ccatgttact ttcgatggtt gctgaacttg tcgcagtacg
     2701 gcctcagtta cgtacttcaa atacgcccct ttgcactcaa tctcagcgcc ttgcatttag
     2761 atacctataa gacgccttgc agtttttagt taaatatttg ttttgagaca gcctctgcaa
     2821 atcccaacat aaaaagctta tcctacaaat gattaaaaga aaaactattt cctaatgatg
     2881 ttattattga attagtcgtt ctgggaatta ttgtttttcc gccgtttctt ttttgaagtt
     2941 ttaccggctt ttatatcaaa tctgtgtata tccccttcat cgagctgagt cgctgcctct
     3001 tcaacttttg cttcttgttg taaggcatgg tcctctaagg attttggata gactccttta
     3061 agattttgct ctaaaatctg ttttacattt tctatagtta aagcaagcat tttcgactga
     3121 tcatcaagat acgaatacca cattattttt ctaaaaacat ccgatttttg atgaatagcc
     3181 tctccttttt cggtttttag ttttattgag ttatctggga attccttcaa agcatcaaga
     3241 taaacatcat tttcaaaatt taaacagcat tttagttttc cgcactgtcc tgccaacttt
     3301 tgtggattca ttggcaataa ctgcgtacgt gctttacttg tcgaaaccga tgtaaaatcg
     3361 gagagccatg atgagcaaca gagctctctt ccgcaagaac ctataccacc taaccttgca
     3421 gcttcctgtc gtaccccgat ctgacgcatt tcaatacgaa ccttaaaatg gtctgctagc
     3481 acttttatca actgcctgaa atccaccctc tcgtcggcag tataataaaa gacagctttt
     3541 gtctcatctc cctgatactc aacatcattt atcttcatat ctaaatttaa atcccacgcc
     3601 atctttctcg atttcaatgt agcttcgttt tcaagatcaa cagtgttaac ccatttttca
     3661 atatcggcta aacgcgcttt acggtaaatc ctttttactt gcggagaata tggatcaaca
     3721 ccttttttta gcatctgaag tttaacgatt tctcctgtga gtgacactat gccaatatca
     3781 tgacctggcg tcgcttccac tgccacaata tcgcctgttg tgaatttcat atcgccatga
     3841 atacgaaaaa aatctttccg acaattttta aatcttactt caacacaatt aaaaagagaa
     3901 tggtcttcgg gcaattgtac attttgcagc cagtcatgag cgtttaactt acaacatctg
     3961 cctacaaaaa tatttttatt atcctgatat aattgaggag cctgagaaca cccacgagtc
     4021 aagaattttt tatctatatc gttatcggat tttatatttt gattttccat ttaattacta
     4081 ttgccaagag ttaatattta taacttgttg aaactttaca aagttacatg aaatcatttt
     4141 atcgttgcca atttgaaaag tcatcggtca aaaatctatc gaacacacct tttaatgcgt
     4201 aatattattt cattttaagc aacttgacaa tcgttagcga aaggtcgaaa aaaacaagag
     4261 ccgagtgtac gttacgttca atatggttta tggctttatt aaattcatta gtaaacatta
     4321 cgatattcag atgatttaca aatggtgaga aattttcata aaacactcgc tcctcctctg
     4381 ctattctgat ataagcatat ttggtatagt tgagcgccag actgttccta atagctacaa
     4441 ggctaaactg caaaaaactt ttattgcttt cgcgaccttc tctggctacc ttttcgctaa
     4501 aatctaacaa tccctgtgca tcgaaagtcc agcacattcg catccaatct ttaaaacgat
     4561 caaaattggc tttcctatct tcgctctgat ccaaaagact tactgcttcg gtaaaactac
     4621 cattagcttg ctgtgcaact tggtttgcta tgggatcgtc aacattaaaa cgttttttaa
     4681 tagcttcggt aatggcatca tcatcaattc gggggatttt aacaggtaaa agtcttgaca
     4741 taatagtgtt aataatctgc tcggtatgct cggcaatcag caaaaagagt gttttatcgg
     4801 gtggctcctc aagaatttta agcaacttgg gagcagctac ataatagagt ttttccacca
     4861 tccaaataat catcactttg taatccgatt cataactggt gtagttaagc gtttgaatta
     4921 tctggttaca ttcacttaca ttgataaccg cctgcttacg ctcaatttca atcttttggt
     4981 accagtcgtt aagtgttata taaccatcgt tttgcaatag tagttcgcgc cattctgtta
     5041 agaaattact acttataggt tttttcgatt cgtcgccacc ggcaatcgga aagacaaaat
     5101 gaagatcagg atgtatgagc ttgttgtact tattacacga tgggcatgtg ccacaagaat
     5161 cgccatttat aaatttattc tcgtccttgc agtttataaa ctgagcatag gcaatagcca
     5221 gtggcagctt acctacccct tcgcctccgt aaaagagctg tgcatgcgca accctgttat
     5281 tctttacagt ttcccgaagt ttctttttca aatgatcctg tcctattatt ttattaaatt
     5341 gcatatccca ttaagatcaa attgcacaaa agtaactgaa aaaaaatcgg ataagctggt
     5401 ttaactaaat ttgcaaaaaa aatgctatta atacgatgtt gctaaaaatt catcctgtaa
     5461 cacccagccc gcggcaaatc ggtaaagttg ttgaatgtct ttacgatggc ggaataataa
     5521 tttacccaac cgatactgtt tacggcttgg gttgcgatat cttcaagtcg aaagctattg
     5581 agcgcattgc ccgcatcaaa ggattgaaac ctgaaaaggc aaacttttct tttatttgcc
     5641 acgacatgag ccaaataacc gactttacaa ggccaattag caataatctg tttaaattga
     5701 taaaacgagt gctgcctggg ccatacacct tcatctttga tgcaaacaac actgttccga
     5761 aatatattca atcgcgcaaa aaaacagtag gtattcgtat ccctaatcat aatatacctc
     5821 tcgaaatagt gcgtcagctt gggcatccta ttatgactac ctccatacac gatgaagacg
     5881 aattgattga atacacaacc gaccctgagc ttatatacga aaaatacaaa gacttggtag
     5941 atattgttat tgatgcgggg tttggaggta acgtaccttc aacagtaatt gactgtactg
     6001 gcgatgagat agaagttgtt cgtgagggct taggtccttt gacaattttt tagcctactg
     6061 aacccacata atatcaatga cataaaacaa agaaattcaa aaaagattaa aactgagttt
     6121 gtcaattaaa aaattggaat tacttttgca ccccgtttta ttcgactgat tcagtagctc
     6181 agcaggtaga gcaacggcct tttaagccgt gggtcctggg ttcgaatccc agctggatca
     6241 caaggaaagc cttgtaatca ttgtggttta caaggctttt tttactctga tacgtcatcc
     6301 tctattcttt taaaagactg atttttgtag tgtaaatagt cgagccatca tttactataa
     6361 ccatgtaaaa acctgatggt aatctgctta aatcaattga aacataatcc caaacagact
     6421 gataaaaagt ttttgaaata attactgaac cgactaaatc aattatctgg acaatgatgc
     6481 agtcagtatc ttttgttctg ataaaaagct cgccttttga aggattagga taaacgtcgg
     6541 cataagaaat tttcaactta ttaatcccgg cgcatacatc aactgtcaat atctgttcat
     6601 ccgttccggt gcatccattt tcatcttcaa aggtataggt tattatatgt tcgccaacac
     6661 cggcggcctg cggatcaaat actccatgct gcacgccgtt tcctgaataa ctacctccta
     6721 aaggatatcc acctataagc cggaaaggcg gatcatactc acaaacagta tcaattggtt
     6781 gcagtgttac cacgggatta gcataaattt ccacaaagtc tgtttcgata gtcgtatctg
     6841 taaacaatgt attggatact gttagcgtaa catcccataa gccaggctca gggtaaaaaa
     6901 tcaaaggatt catttcgttt gatgtaggcg gtacacctcc ttcaaactgc cagtcccagt
     6961 ttgtaatgtt tccataggac tcattattaa actgaatata gctaccctga caaacctgat
     7021 tcacttcagc gctaaaatcg gctattaatc ccatattaca aaactgaatg taactgtcta
     7081 tacattccat ttctggtggg aaacaaatat caaaatgcat tttataatcc ggacaaatga
     7141 ccaaataagt gggggttcca taatatatct gtccatcaat caaaacgtca atagcctccc
     7201 cggcatttcc ttcgctgcct gcacacgggt ggctaactcc atattgcgtt ttaaactcct
     7261 caatcacgaa attactgtcg taacgcgaaa gaccccaaac gttcaaattt cccattccgg
     7321 agccatacct gatgtacact tcttcaatta atggagcata caccgtgcat gacattcagg
     7381 tagcctgaaa aatatcaata aaaattgtgt tgcccttatc caactcatcg taaagcgtcc
     7441 aattgtagcc atccgaatcg gttattgtaa agtttccaac aacttttaat tccgtcaaac
     7501 catcagtgga tgtaacctca aaagtcctgt cctgagcaga tacagataac ataaacatca
     7561 gaagggcaat agcaaaaaat agttttttaa ccatagcttg taaattgtca attaatttaa
     7621 aacttaacag attattaacc agtaccctaa ttttgattta ttaatattca tttagcctat
     7681 ctttgacgtc taaaatactg atactgtaac aggttttaac atgaaaaata ttaatttgtt
     7741 cacactcatt ttattatccg ttataatctc ttgtacttca tcgcacaaca atacaaaaat
     7801 caaatttata ggcgaaactc agggtactta ctatgcaata acctattttg cttcggatac
     7861 tttaatagct caagttcagg ttgactcgct tctcgctgat ttcgacatga tagcctctat
     7921 ttgggagcct aattcgcttg tttccaaaat taatagaaat gaaaccgatg aagcaaacaa
     7981 tctccatttt gaagagatgt ttaatatttc acaaaagatc tctgccgaga ctgaaggttc
     8041 ctttgatatt actgttgggc cgttggttaa tgcatgggga tttggattta aaaacaaaca
     8101 agatcttact aaagaaatgg ttgatagtat tatgcagttt gttggttatc gaaatgtgag
     8161 gttagaaaat ggaaaaatca taaaggatca tccaaatatt cagattgacc taaacgcgat
     8221 tgctccggga tatgcagtag atattttagg aaaatatttt gaatccaaag gcataaaagt
     8281 ttatctgatt gacataggtg gagaaattat aactaaaggt ttaaaaccca atggtgaaaa
     8341 atggattgtc ggcatcgaaa agccgagcga agaggcaaca agcgggcgaa tattgaaggc
     8401 gaaagttaaa gtagcggata tcggactgac aacgtcagga aactatcgaa aattttatat
     8461 taaagatggc gtaaagtact ctcacaccat taaccctcat acaggatacc ctgtaactca
     8521 ttccttgctt tcggcaacgg tttttgctag taatgctgct tttgctgatg ggtatgcaac
     8581 agcttttatg gtcatgggat tagaaaagac aaaactcttt ttagaaaaaa ataagaatct
     8641 cggagtttat ctgatttata ctgatgaagc agggaactta agaactttta ccacacccga
     8701 tgtggaaaag atgcttaatg agctttgatc tcctcaaact caatatcttc ataccgcttt
     8761 ttattgttac agcgctcgtc atccattttg ccgcaagtgc atgcaatccg ctttccagtg
     8821 gtaggatcaa cgctaccaca ggacttttgg aactcgccgc cttttttaag caacatctta
     8881 atagcaatgc caatgaagga cagccccaca aagacaattg aaatgaggaa tacttttaac
     8941 atgacttttc tgtattacgc aattctttaa caaaagaaaa aagctaagtt taatcgaatt
     9001 tttcaaataa aacagacgct ttaagcgctt tgcgtctaaa ctcggaacaa atcactgctg
     9061 tagctactga tgcatttaac gactcggcag cagaggaaaa cgatgggata gttataagaa
     9121 agtcaacctt ttcttttact tcagcagaga ttccatgcga ttcgccccct atcacaatta
     9181 cagctttttc aggcagctcc ttagcataaa tgttaacacc gtcaagtgtg gctccataaa
     9241 tgggtatatc cgaacagtat ttttgaaaaa tttccggtaa aaacccataa aacacattta
     9301 ctctaaacaa tgagcccatt gttgcttgta ccactttcgg gttaaaaagt tcaaccgaat
     9361 ccgatgaaca tattacggtt ttaataccat accaatcagc aatccttatt atagttccaa
     9421 ggtttcccgg atcagcaata ccatcgagca taagcacaag attgttaaag gcctccaggt
     9481 ttaacgaagg ttctgggatc ctgaccaaag ccaaaacatc atttggtgtt ttcaaagcgc
     9541 taatgcgctt tagttcggca ctcgatatct ttattacttc aaacttatct ttaaaaaatt
     9601 cacccctgtt tttaacaaac catttttcag tagcataaat agcttcaatc ccataatctg
     9661 acctcaaaag ttctgcaacc attttattgc cctcaacaag aaaaagattg gccgattttc
     9721 gaaacttttg taaatgtagc gatctgatta attttatttg tgctgccgat aacatctttt
     9781 ttatttgata acaacagcaa aagtaatcta tctaaaagaa gtctttcaaa aataaattaa
     9841 tattggctga acactaacgg aaagaatatg tttcttaaat aaaatcagtt tataactaaa
     9901 cactttaaac aatggcaaaa atcacattaa aaggtaatcc tattaatact gtgggtaatc
     9961 ttccggtcgt cggatcaatt gcgccggatt ttaaacttgt taaatccgac ttaagtatat
    10021 tatctcttga ggatttaaag ggtaagcgtg tggtattaaa tattttcccc agcttagata
    10081 ccgatgtttg cgcaacatca gtacgtcgct ttaacgctga ggcttcaaaa ctcgataaca
    10141 ccgttgtagt gtgtatctcc aaagatcttc catttgctca ttcacgtttt tgcgccaccg
    10201 aaggactaaa caatgttgtt acagcatctg attttaggga tggaacgttt ggcattagtt
    10261 atggtgttac catagctgat agcccgcttg aaggtttgca ttcgcgcgca gtggttgtgc
    10321 tcgatattaa tggtaaagtg aaatatactg agcaggttcc agaaattgct caggagcctg
    10381 attatcaagc agcgcttgca gcgctataaa ttttgaatat taccattttc tagtgctgaa
    10441 ttattaagaa aatggccaaa gcagaataaa aaaatgccgc tcggatcgtt tccagcggcg
    10501 ttttttatag tgatggttta atatctattc agcgtaaact tcaaatttaa gttcggcttt
    10561 tatatcacga taaaggtgaa ccttggcaat gtaattacca agttctttga tattttcacc
    10621 atcaacgtcc acttttttcc ggtcaatatc aaatcctttt tctttgagcg cctctgcaat
    10681 ttgtattgca tttaccgaac caaatatttt gcctgatgta ccagcttttg ctccaatcct
    10741 tagttctatc ttttcgatta atttttgggt tgtctcagca tctttacgca gtttttgttc
    10801 tttaaaatgt ttttgtcttt gtacttcagc taagattttc ttgtttgtat cggtagccaa
    10861 aatggccatt cccttaggga tgagatagtt tctggcatat ccatttttta ctttcacaat
    10921 ctcgttttcg taaccgaggt tttcgatgtc ttgttttaaa attacttcca tgatttcctc
    10981 ctcctatttt agtaagtcag caacgtaagg taacaaagcg aggtgcctgg cacgttttat
    11041 tgcttgtgca accttcttct gatattttgt agatgttcct gtaatgcggc gaggtaaaat
    11101 cttaccctgc tcatttacaa acttcaataa gaagttttca tctttataat caatgtattt
    11161 gatccctaat tttttaaagc ggcaatattt tttctttttt acatcaaccg ctattggggt
    11221 taaaaatttt atatcggaat tttgttgagc catgatgtta atgtttaagg attaattaat
    11281 ttgttcttct ttcaccggga cttcatcttt tgctttagca cgtttttttt cgttataagc
    11341 aattgcgtgt ttatcaagtt tcacagtaag aaaacgaatt acacgttcgt ctcttttaaa
    11401 ggccagctca atttcaaaaa tcaactcgcc ttcagccttg tactcgatta ggtgataaaa
    11461 tcctgtggat ttcttttgaa ttgggtatgc aagcttacgc atgccccaat gttcctcgta
    11521 aacaatctca gcgcccttgg acttcaacaa gtcctggtac tttcttaccg tttccttcat
    11581 ctgttcatca gacaaaacgg gagttaaaat gaaaacggtt tcatactgat ttaacatctt
    11641 gtttttgttt tagttaataa taaaattata atttttaaag tgggcgcaaa aatagtatta
    11701 ttatttgtat ttagatattc attttcattt tttttacaaa atgaggccaa tgcttttaaa
    11761 cagctaacga ataaatcgta cccaaaccaa cgataactaa cagaagatat gattactatt
    11821 ccggtttacc gatgaaaaaa tcgaatgaaa agtacgaata atcaaaagaa tctaattcgt
    11881 ttaaaagaag aaaaaaacgt ttacaaatca tgtttctttc caaaaaatat aaacctgtaa
    11941 aaattgacga gtactctttt acctttaatt tgcgaaagca atactccagt taaaaccaaa
    12001 gcaattccaa taatcttctc ggcttcaaaa acttccttta aaacaaaaaa tgaaaaaaga
    12061 gctgtaaaaa ccggaatcgt atttgaaaaa aggctagttt tacttattcc aatctcacga
    12121 gctccgatag tgtagaaggc aaatgcgagt gaggaggcaa aaaaggcgag aagcaacagt
    12181 gaaacaagca tctcggcatt aggtctgaca gtaataaaat ggcgaaaatc gagaataaaa
    12241 aatagtggaa ggaagtaaaa aatgccgatg aaattttgta ctgcaataat attaaacgca
    12301 tcatatttaa aagccagctt ttttaagaat accgcataca aaacagccgt tattacagca
    12361 agaaaaagcc atgcaacacc cactggcgaa gcagttaatg aaaaatcttt gtctatcagc
    12421 attataatca ctccaaaaaa agagatcaat aaaccgacaa tattaagcca agtaagcctc
    12481 tccctaaaaa ttatatatcc tacaataggt gtaaaaagtg gaatagtacc aataattaca
    12541 gatgaaaccg ttggtgatga gtattttaac ccataattct cgctcagaaa atataaaaaa
    12601 ggattgaaaa atgctgataa aaggaagagc ttataatctt cttttttgat cttttgcaac
    12661 cgattaaaca acttaagccc gccaaacatt ataagcgacg ataggatcaa tcgtaataag
    12721 acggtagtta tcggatcgta atatttcaat aggatttttg ataacacaaa actaccaccc
    12781 caaaatatca ttgaaagggt gatgagtatg tatgtataaa ccggcttgag cttcaagaga
    12841 aagacattat tttattgaac cgagcaaaag taggcagaaa agcccaataa caacattata
    12901 ttataaagag tacaagtata aatccatttt catctgatta tttttcggaa ttctgtccac
    12961 ctcctccaaa tctttctact aaactaattt cgattatatt tgtcctgaaa aatattagca
    13021 ttatgcaaaa tctcaataaa aaattagaca gtgctgcgga ggtcgtttcc agatcaaagt
    13081 atcttgttgc ttttaccggc gccggtattt cagtagagag cggtatccca ccctttagag
    13141 gcgctgatgg actttggagt aaatacgatc ccatggttct cgaattaagc tatttttatc
    13201 ataatcctct tgaatcatgg aaggtaataa aggagatatt ttacgatttt ttcgggaaag
    13261 cagcgcccaa caaagctcat ttagcacttg ccagattaga acagcttggc attcttaagt
    13321 cggtgataac ccaaaatatt gataacctgc atcagttggc cggaaacaca agaatatttg
    13381 aatttcatgg aaactcacaa aggctaattt gtacaaattg caacgaaatt catattccgg
    13441 ataaggttct attggaaaac cttcctcctc tttgtgcaag ttgtaatagc ctacttaaac
    13501 cggatttcat tttttttggg gaacaaatac caatggatgc ctataaatac tccattgaag
    13561 ccgctgaaaa atgcgatgtg tttctgatca ttggctcaac cggtgaggtg tcgccggcaa
    13621 atcaaatccc atcaatagcc aaacgcaaag atgcaacaat catcgaaata aacccggaag
    13681 aatcgcacta cacttcaacg ttaacagata tttacctctg tggtcgggca gtagagataa
    13741 tgcaactttt aatggcaagg gtggaaaaat taatagaaag aaactgatag attatttttc
    13801 gttatttttg ccacattata tttttcgcgg tagtagctca gtcggtagag catcagcttc
    13861 ccaagctgag ggtcgtgggt tcgagcccca tttaccgctc aacatctgat cctgcaagca
    13921 aatgcggatt ttttccaatt caggcaatac tgatttcaga aaaaagcact atcaaggctt
    13981 atcataatca gcttgacaat cactttggtc gccagcaatc actatttcgt agttccaaac
    14041 tatctttccg gctgacttaa gataacccga accttttaca gtccagtcat caccaattgt
    14101 ctggctaggg atctcaacaa cattcccctt tacaaaacca taggctggcg aatattccaa
    14161 tccggcaagt gcaaagttgt aaagaaaaac cttggtgtcg ctatcagtat cggctttaat
    14221 ctcaacttca taattaaacc ttacacatgt ttcgcttact acccaggttc caagaaaagc
    14281 agcacgctca tcttcatcag taaaaacatc atcaacacag gaggtaactc ctaaaagcag
    14341 gaaaaacgat aaaataaaaa acgatatctt tttcataagt ttttttaagg aatcaaaact
    14401 ataaaaaata tgccattttg atttattttt agaaaaaaag ttgataaaat cgaaataagt
    14461 aaactaataa tactggcact atttatcatt tcttagccaa tttatatcct gtgtcgttaa
    14521 gattcaattg gttaattacc tcagcagtat cagcaagttg agggattgct ctgaaagaga
    14581 caaaacttca aaaatcaagt ataacaaatc cagctttaag caaagagttg atcagctcca
    14641 cttttttcac aatcgggata acgcttttat tccttgaaaa acgttacaat acaactcagt
    14701 aatttttatc agtaatggat tatctatatc tttctgattc gttcactaca taaaattaat
    14761 caatcaggtt tgccaaatga cttaaatttg caaggcttta acaaaatatt aatttatcta
    14821 aattacatga gacatcagcc tattgacacc aaattcactc atgaagaagt agaaaaagag
    14881 atccgatact tacaacttct ttccaaacaa tttcctactg ttcaaactgc cagcactgaa
    14941 ataattaacc ttgaggctgt actgcagtta cccaaaggca ctgagcattt tctttcggat
    15001 atccacggcg aacacgaaat tttcagccat gtactacgta atggctccgg cgttatcaga
    15061 caaaagattg aagatgtttt taaaaatacc cttcgaaaat ttatcaagga cgagcttgcc
    15121 acactaatct attatccaga ggagaaactt gctttgatcg aaaaaagtga atcagatatt
    15181 gaagaatggt attcagttac gcttcaaaga ttagttgaag ttacacgagt tgcagcatcc
    15241 aaatataccc gttcaaaggt cagaaaatcg ctacctaccg attttgccta tatcatcgaa
    15301 gagttattac acgaaaagcc aagctcgcct aataaagcga agtattttga cgagataatc
    15361 caaactataa tacgtattga cagagctaag gagtttatca ttgctatttg caagctgatt
    15421 cagcgtttat ccattgacag actgcatatt gtcggcgaca tatttgatcg tggcccagca
    15481 gcggaaaaag taatggagat gctgacaaac tatcattccg ttgatattca atggggcaat
    15541 cacgatatca tctggatggg aagtgcagca ggaataaggg caatgattgc caacctgatc
    15601 cgtatctctg cccgatacat gactttagat accattgaaa acggatacgg gataagtctc
    15661 ctaccattgg ctacatttgc tatggagaga tacaaaaatg atccctgtaa cctttttacg
    15721 ccgagaatcc cggaaggcgc agaggcaagc accaaagaaa tagaaatgat caaaaagatg
    15781 cataaagcta ttgcaattat gcagtttaag tatgaatggg aaatcataaa acggaatccg
    15841 caatttgaaa tggataatcg taacctactt gacaaaattg acttcgcaaa tggcactctg
    15901 gaaatagacg gtaagaatta tcagatgctt gacaacaact tccctacggt tgatcctaaa
    15961 aatcccttca agctaacaga agaagagatt gaagtgatgg aaaaattgcg aatatcattt
    16021 atgcacagtg aattactaaa gaagcatgta aaatttctat attccaaggg tagtatgtat
    16081 ctgagatata acgacaacct gttgttgcat ggttgtgtac caatggataa tgatggaaat
    16141 ttcattaaga tgatattatg cgaaaaagaa ttgagtggaa aggctttgtt agaccagttt
    16201 gatctctttg cccgcaaagc atattttact cgtaacgaca agcagataga tacaattgcc
    16261 catgatcaca catggtatct gtggtgcggc aaatattcac cactttttgg aaagaaaaag
    16321 atgacaactt tcgaaagata tttcatcgca gaaaaaggga cgcatattga agaaaaaaat
    16381 gcattttaca aacttaatga taatgaggat gttataaaaa agattttggc tgagtttgaa
    16441 cttaatcccg aaattgccca ccttatcaac ggacacgtcc cggtaaaagt aaaagcaggc
    16501 gaaagccctg ttaaggcaaa tggtaaatta ttggtaattg atggcggcat gtcaaaagcc
    16561 tatcaaaaag taacggggat atcgggttat acgctaattt acaactcata cgggttagta
    16621 ttagtagctc accaagcttt tgaaaacaca accaaagcaa tcgaagaaga gtctgatatt
    16681 ctttcgaaca tcacatattt agcatctaat ccaacacgaa aaactgttgc tgatactgac
    16741 aacggcaaaa tattaagaca acaaattacc gatctggaaa tgcttcttgc cgcctataga
    16801 aaagggttaa taaaagagat aagataatgg tttatatttt tttatctgaa aaatcatttt
    16861 gtcaaattaa aaaattgatc tacttttgca gcccatttga aaaaagtaaa atttaaaaag
    16921 atatgtactt aaccaaagaa caaaaggcga gtttcttcga aacatatgga aaatcgaaac
    16981 aaaacaccgg atcggccgaa ggccaaattg ctctttttac ctatagaata aagcatctta
    17041 cagagcattt aaaagaacat aaaaaagatt taaatacacg gcgatcattg gtacttttag
    17101 taggtaa
//
LOCUS       DAOOAI010000045        16464 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_4588_length_16464_cov_4.519776, whole genome shotgun sequence.
ACCESSION   DAOOAI010000045 DAOOAI010000000
VERSION     DAOOAI010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16464)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16464)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16464
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4588_length_16464_cov_4.519776"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(94..972)
                     /locus_tag="PK345_04695"
     CDS             complement(94..972)
                     /locus_tag="PK345_04695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34350.1"
                     /translation="MKKRLTKLIILSVVFVFLSLIFACQQENLKPGSTAFIEVDHFNL
                     ATNYITNGSNSHKITDVWVYADDQTIGVFELPITIPILKSGIGKLRLEAGIKLNGIKT
                     TRVNNPFYEPVIIEPFNFIIDSTILLSPTTTYRPQTVFVWMEDFESDVISLDTTRLTS
                     DVTVVKSTSSQAFEGSSSGMIALNKDNNVFEAATFEAYVLPTTGKPVFLEMDYKNDFN
                     FTVGIFSQTENQILKKEVIYLLPKEDWNKIYINLTDKLTESQNAIDFKVFFRTALPEE
                     IDTATIFLDNLKLIYR"
     gene            complement(1025..2020)
                     /locus_tag="PK345_04700"
     CDS             complement(1025..2020)
                     /locus_tag="PK345_04700"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPX34351.1"
                     /translation="MLKIGVLGAGHLGKIHLKCISQIDQYKLVGFYDPNKETAEAVAN
                     DFQIKRFNNLHELIDAVDVVDIVTPTVSHFSCAVEAIKKSKHVFIEKPIVTTLTEAHE
                     LIELAQEAGVKVQVGHVERFNPAFLAARPLIQNPMFIEAHRLSQFNPRGTDVPVVLDL
                     MTHDIDIVLSVVNSGIRKIHASGVAVVSDTPDIANARIEFDNGCVANLTASRISMKNM
                     RKSRFFQRDAYIAVDFLKKDIEVVRMRDIETEPDDPFAMIIDLEQGKGKKEIMLDKPE
                     IIHNNAIVTELQSFADAIQNNVTPPVTIYDGYKVLEISHMILEKINSKAYFKPLF"
     gene            2112..2765
                     /locus_tag="PK345_04705"
     CDS             2112..2765
                     /locus_tag="PK345_04705"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HPX34352.1"
                     /translation="MELSDSYKHKGLRQHLINNLRIKGIKDENVLDAMMNVPRHHFMD
                     SSFIEYAYQDKAFPIGSGQTISQPYTVAFQTELLNVEPGHSVLEIGTGSGYQAVILYN
                     MGAKVYSIERQYNLYRKVKLFFDRYNYKIKVFYRDGYDGLSTFAPFDRIIITAAVPLV
                     PEALKRQLKIGGILVAPVGQKNHQTMIRLIKKGEGEFITEEHGGFIFVPMLPGKAED"
     gene            complement(2762..5185)
                     /locus_tag="PK345_04710"
     CDS             complement(2762..5185)
                     /locus_tag="PK345_04710"
                     /inference="COORDINATES: protein motif:HMM:NF016488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPX34353.1"
                     /translation="MIRTIEEKEIDKKFETLKQFCTNCTPDELDKLDAAYRYAAKVYG
                     DKINSSGELLISHSLSVARIVAEEIGLRSVSLIAALLHDVINTADDNCEEIESKFGKD
                     VATIVRGFKKVSVIDSEKVKLHSENFRSLFLSMIDDVRVIFIKIAHRLYDLRNYSSLT
                     EFQKKIFLNDVQYLYIPITHRMGLYHIKAQLEDMAFRHSYPVEYSKIESRLHETKEQQ
                     EKYIEKFIRPIKEQFDKSGISYEIKSRAKSITSIKRKMEAQDIGIDKVYDLFAIRVIL
                     QNVLSDEDDEFIEDYLANWEKFGDQRSIKRKRIKEKEEQEDSSSENRKKQKDGKEKSL
                     ESINENQENEIKDFEDRKKRYLELINREKTACWQAYSLIANTYDPNPKRLRDWITTPK
                     LSGYESLHTTVLGPEDRWVEVQIRTERMDVNAEKGSAAHWKYKETAYGKNIDNWLLDV
                     RNLLETIGTQRFDEQKQTKIDHAASNIYIFTPNGDLRELKNGATILDFAFEIHTDVGS
                     KCVGGKINGKIHPIRYQLKNGDKVEIITAKNQRPNPDWLNVVVTSKAKSRIARALREQ
                     KFKEAEIGKDILYRKFKNWKIELNERELNKIIKFYGFKLPVDLYFNIATGKIDLQDVK
                     QIFKTSDEIEERISTTEKPINVEELIENRSEKDLGYITIESGVSNLNYSLAKCCNPIA
                     GDRIFGFVTVNQGIKVHRMDCPNAEQMLTKYPYRVIKARWKESQSTKVFITNLRVIGM
                     DRVGILNDITKVISEDLRVNMKSVNFKSAGSGFEGVIKVQVRDVEHLGYLRQRLLKIK
                     GVIKVVRFD"
     gene            complement(5387..6391)
                     /gene="floA"
                     /locus_tag="PK345_04715"
     CDS             complement(5387..6391)
                     /gene="floA"
                     /locus_tag="PK345_04715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019150166.1"
                     /note="flotillin-like protein involved in membrane lipid
                     rafts; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin-like protein FloA"
                     /protein_id="HPX34354.1"
                     /translation="MESNVLIIIAIGVASLFGLWLIFYFIPVGLWFSALVSGVRISLL
                     QLVLMRWRKIPPAIIVNSLIAATKAGLKLNRNELEAHYLAGGRLKSVVNALISADKAN
                     MGLDFKAATAIDLAGRDVFEAVQMSVNPKVINAPPVAAVAKDGIQLIAKARVTVRANI
                     RQLVGGAGEDTVIARVGEGVVSAIGSANSHKSVLENPETISKLVLNKGLDSGTAFEIL
                     SIDIADIDVGKNIGAVLQMDQANADKNIAQAKAEERRSMAIALEQEMKARAQEARANV
                     IQAEAEIPKAIAEAFRNGNLGIMDYYKFENIKADTRMRDSIAGAPETQTKSKQKPEGK
                     "
     gene            complement(6396..6863)
                     /locus_tag="PK345_04720"
     CDS             complement(6396..6863)
                     /locus_tag="PK345_04720"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HPX34355.1"
                     /translation="MSWTVIIVLILVGLLFLLLEALVIPGTTIVGVIGFVLIAIGVWQ
                     SYAIHGAVGGNIVLLSTVLLSVVVLILALRSKTWKRIMLHTELDGKTNIVEEESVQVG
                     DVGKTISRLAPMGKAFINNQYFEVTSTGGLIDQETEIRVVKIEGKKIFVKLNT"
     gene            complement(6881..7162)
                     /locus_tag="PK345_04725"
     CDS             complement(6881..7162)
                     /locus_tag="PK345_04725"
                     /inference="COORDINATES: protein motif:HMM:NF014655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adaptor ClpS"
                     /protein_id="HPX34356.1"
                     /translation="MKKMIKERTKSLDQNEEQLTDRRELILFNDDINTFDFVIETLIE
                     VCEHDPLTAEQCTLIAHFNGKCSVKSGDVEELIPISSEMTNRGLTVSIK"
     gene            7259..7900
                     /locus_tag="PK345_04730"
     CDS             7259..7900
                     /locus_tag="PK345_04730"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - CoA pyrophosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HPX34357.1"
                     /translation="MEPMQFDQLIKRLEHSLKQPLPGVKAHTRLFPPTRSEFPPINFS
                     AVKIGAVLAMLFSQNGQTRLTFILRQKYNGVHSGQISFPGGGYEENDLTYSKTATRET
                     QEEIGVPEEEIKIIGQLSQLYIPPSNFLVYPFVGYIQKQPNYKPDITEVTEVFDLSLN
                     ELMKEECFQFQPVISRSKQVKVPCFVTQNRLIWGATAMILNELLEIIKQGSDF"
     gene            complement(7924..8340)
                     /locus_tag="PK345_04735"
     CDS             complement(7924..8340)
                     /locus_tag="PK345_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPX34358.1"
                     /translation="MDKYAEVTLNRGMSFDVEVNGHKFLIDADEKVGGENRGPRPKPL
                     LLAGLGGCTGMDVISILRKMRVEPEYFNVVVLAESTNEHPMYYKKIHIEYQFRGKDLP
                     MDKLEKAVNLSQERYCGVSFMLGKASEITSEIKILD"
     regulatory      8692..8895
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            9261..11216
                     /locus_tag="PK345_04740"
     CDS             9261..11216
                     /locus_tag="PK345_04740"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPX34359.1"
                     /translation="MANEVRLNHLIIKSTFIVVFIVFWLTLPAQNHKDTLHLETVEIV
                     DQRLEQVLGYHETKLTKRLVTDRVSVNLADFLNVNTSIFIKSYANGGLVTTSSRGASA
                     SHTEVLWNGINLNSTMNGNADISQIPLLFVDKINVGYGGGISTYNSSGLTGAIDLATE
                     TNWQNRFSLELKQDISSFNTYRTYFLCNVGNYKFQSSTRFFYETSENNYPFLNNTLNG
                     EKPQKEYRTNASFDQTGLLQELNWNLASNTNISLKAWIQENDRNLPSNILTKLPNEKE
                     SSYDQIARAVVEMKQQFYNSKLEFTSGIFQSWSNYKKKTAGINSDHITFTSANKLRYK
                     YSGFKNLMILTDVGFDFHNVKSDNYDSKRERGEGKLKLNLQYFGIKKFVFNGSVIQKL
                     VDHTAVPLISSFGIGYRVREKNPIILNASVTRNIRIPSFNDLYWTPGGNPYLKNEKGI
                     IAETGITIERNYRKTNLKGSISLFHNKIDDWIIWQPDTLFSYWKPFNLKKGISQGIEA
                     ELTASGALNQLKWNYSLTYAFTSAKNKSRTSGSDKTINKQLIYVPKHIANQSILVSFK
                     SMSASYVISYTGKRFTNPDHYSYLQAYFLQDFAISKSFELGGSTLNCQFTIHNIFNQQ
                     YQIIAWYPMPGRHLSFSIKYNFKNSKS"
     gene            11213..12310
                     /locus_tag="PK345_04745"
     CDS             11213..12310
                     /locus_tag="PK345_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YncE family protein"
                     /protein_id="HPX34360.1"
                     /translation="MNRKKSTFDFSRLALIWALFLAVNAIMLYSCRDDYGEIKKNTSL
                     YSKGKGLFIVNEGNFGNGNGSISFFSLDSLKMYNDIFYSANNRPLGDVPVSITLLEEE
                     SWIVVNNSGKIEIVKNSDFSSVTTITGFTSPRFLLKVSDKKAYVSDLYSDQITIVNVK
                     TKKIEGHIPIKRSSEQMVMVGGRVFSAFWSNFNFPEFENNQLMIIDIEKDIVVDSIQV
                     GKEPNSMVLDANNKIWVLCSGGYMSEETATLHRINPTTLQTEVIHTFSDPFASPTSLC
                     INATGDTLLYLNQGIFRMSVTEDNLPETAFIPQNSRLFNTLGIDPMTSEIFVTDAIDY
                     QQHGLILHFSRNGELKGTYSAGIIPGNLCFN"
     gene            complement(12450..13648)
                     /locus_tag="PK345_04750"
                     /pseudo
     CDS             complement(12450..13648)
                     /locus_tag="PK345_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005930543.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
     gene            complement(13738..14193)
                     /locus_tag="PK345_04755"
     CDS             complement(13738..14193)
                     /locus_tag="PK345_04755"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPX34361.1"
                     /translation="MKRILMLSLGIMFSISMIAQELQTKEQPAENPNAPVITFEKTVH
                     DYGTVPYNGDGKCEFEFTNTGKEPLILTNVRSSCGCTVPKWPREPILPGDKGIINVEY
                     KTNRIGAINKSVTVQSNASNSTVFLKITGQVLSQQQSAAPEKPKSVGEK"
     gene            14324..14857
                     /locus_tag="PK345_04760"
     CDS             14324..14857
                     /locus_tag="PK345_04760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34362.1"
                     /translation="MNKKYILLSAIFIAFLFLSYSCCTSRNQKKASSITQENTPPKLP
                     SPAVIIYKTKGDYNYNVPVILSVDKTRIISFPDPKDIRIDGNFTYPDELVDGYLLDKR
                     GINEHAAFLKFTYEDYYNMDNIPTAERLMNYILDDDPFIEIYDAGKKGQYKNIIDDLN
                     TMILQGKIREMKNLLKK"
     gene            complement(15019..16038)
                     /locus_tag="PK345_04765"
     CDS             complement(15019..16038)
                     /locus_tag="PK345_04765"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012458011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 - branched-chain-amino-acid
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HPX34363.1"
                     /translation="MTNIDWKNLPFGYFKTDYNVRCYYRNGKWGEVEVSSSEYITMHM
                     AATCLHYGQEAFEGMKAFRGSDGKIRVFRWMENAKRMQRSAEGIMMAVPPVELFGEMI
                     FKAIKLNEKYVPPYGYGASLYLRPLLIGTGAEVGVKPADEYLFMVFVGPVGPYFKEGF
                     KPVNFQIISDYDRAAPFGTGHIKVGGNYAASLKALIRGKNEGFPSVIFRNSADRRYID
                     EAGPANFFGIKGNSYITPDSPSILPSITNLSLRQLAKDIGLNVELRSVAVDELDSFEE
                     AGACGTAAVITPIGKIVDRDTGKVVEYSKDGKAGPISTKLYNKLKGIQEGTEEDIHNW
                     NTVVE"
BASE COUNT         5175 a         3340 c         2742 g         5207 t
ORIGIN      
        1 agataaaaaa tagcaaccaa gcaagaattg cagcaatcca atctgcaaaa agaaatttag
       61 ctgtctgggt agttttattc attgtataaa gttttaccga taaatcaatt tgagattatc
      121 caaaaatata gtagcggtat caatctcttc cggtaaggct gttctgaaaa aaactttaaa
      181 atcaatcgca ttctgacttt cagtcaattt atctgtcaga ttgatgtata tcttattcca
      241 atcctctttg ggaagtaaat agataacctc ttttttcaat atctgatttt ctgtctgtga
      301 aaaaatacct actgtaaaat taaagtcatt tttataatcc atttccaaaa aaacgggctt
      361 acctgttgtt ggcaaaacat aagcttcgaa ggtagctgct tcgaatacat tattgtcttt
      421 atttaaggcg atcatacctg aactgcttcc ttcaaatgct tgtgaagacg ttgattttac
      481 aactgtaaca tcactggtaa ggcgtgttgt atctaacgat atcacatcgg attcaaaatc
      541 ttccatccac acaaaaacgg tttgcgggcg ataggtagtt gtcggagaaa gtaaaattgt
      601 gctatcaata ataaaattaa aaggttcaat tattacaggt tcataaaagg gattattaac
      661 tcgtgtcgtc ttaatgccgt ttaatttgat gccagcctct aaccttaact tccctattcc
      721 acttttcaaa atagggatag tgattggaag ctcaaaaaca ccgatagttt gatcatcggc
      781 ataaacccat acatcagtga ttttatgcga atttgacccg ttagtaatat aattagtggc
      841 gaggttgaaa tgatccactt cgataaacgc tgtggagcca ggttttaagt tttcctgttg
      901 acaagcaaaa attaaagaga gaaaaacaaa tacaacagat aagattatta gtttagttag
      961 tcttttcttc aagattatat gaattgatta aagataatat tcaaattatt agatgaaaaa
     1021 taagctaaaa cagtggcttg aagtatgctt ttgaattgat tttttctaaa atcatatgcg
     1081 atatttcaag aactttgtat ccatcgtata tggttactgg cggcgttaca ttattttgta
     1141 ttgcatcagc aaaactctgc aattcagtaa cgattgcatt gttatggatt atttcgggct
     1201 tatcaagcat gatctctttt tttccctttc cctgttccag atcaataatc atggcaaacg
     1261 gatcatctgg ttctgtttcg atatccctca tcctcacaac ctcgatatcc tttttcaaga
     1321 agtcaacagc gatataggca tcgcgttgaa aaaatctgga tttgcgcatg tttttcattg
     1381 atatcctgct tgcggtaaga tttgccacac atccgttgtc gaactcaatc cgtgcattgg
     1441 caatatcggg agtatcgcta acaacagcta ctccacttgc atgtattttc ctgatccctg
     1501 aattgacaac acttaacaca atgtcaatat cgtgagtcat caagtcaagc acaaccggca
     1561 catccgttcc ccgcggatta aattgtgaaa ggcgatgcgc ttcgataaac ataggatttt
     1621 ggattaacgg acgagcagca agaaatgcag gattaaaccg ttcgacatga ccaacctgca
     1681 ctttcacacc agcctcctgc gccagctcaa taagctcgtg ggcttcggta agtgtagtaa
     1741 cgataggctt ttcaataaaa acatgtttcg acttttttat tgcctcaaca gcacaactaa
     1801 aatgtgaaac agtaggtgta acaatatcta ccacatccac tgcatcaatc aactcatgga
     1861 gattattaaa tcgtttaatc tgaaaatcat tggcaactgc ttcagctgtt tctttattcg
     1921 gatcgtaaaa acccactaat ttgtattggt caatctgaga aatacacttc aaatgaattt
     1981 taccaagatg acctgctcca agaactccaa tcttcagcat aatcaatttt ttggcaaaaa
     2041 taggagattt atgcttgtca aataagtatt ttcgcaggca ataagtgcaa ccttctttaa
     2101 aaaaatctta aatggaacta tcagatagct ataaacacaa aggattgagg caacatttga
     2161 tcaacaattt aagaatcaaa ggtattaaag atgaaaatgt tctcgatgcc atgatgaacg
     2221 ttccgcggca tcattttatg gactcttcgt ttattgagta tgcctatcag gacaaagcct
     2281 ttccgattgg atcggggcaa acaatctctc aaccttacac agtcgctttt caaacggagt
     2341 tattaaatgt tgaacccgga catagcgttt tagaaatagg caccggttcg gggtatcagg
     2401 ctgtaatact ttataacatg ggcgctaaag tatattctat tgaacgccag tacaatctgt
     2461 acagaaaggt aaaactattc tttgatcgtt ataattacaa aataaaagtc ttttatcgtg
     2521 atggatacga tggcctctca acttttgccc ctttcgacag aatcatcatc accgctgctg
     2581 tgcctctcgt ccctgaagca ttaaaacggc aattaaaaat cggaggaatt ttagtcgctc
     2641 ccgtggggca aaaaaatcat caaacaatga tcagattaat aaaaaaaggc gagggggaat
     2701 ttataactga ggagcatggc ggttttatat ttgtacctat gcttcccggc aaagctgagg
     2761 actaatcgaa acgcacaact tttattactc ctttaatttt tagcaatcgc tgacgaagat
     2821 aaccaagatg ctctacatca cgcacttgta ctttaattac accttcaaat cccgaccctg
     2881 ctgacttaaa gtttacactt ttcatattta cccgaaggtc ttcgcttata actttagtaa
     2941 tatcatttaa tatcccaacc ctatccatcc caatcacacg taagttagta ataaaaactt
     3001 tggttgactg cgactctttc caacgtgcct ttataaccct gtaaggatat ttggtcagca
     3061 tttgttcagc atttgggcaa tccatacgat gtaccttaat cccctggttt acagttacaa
     3121 aaccaaaaat acgatcgccc gcaattggat tacaacattt ggcaaggctg taattaaggt
     3181 ttgacacacc cgattcaata gtgatatagc caagatcttt ttcgcttctg ttttcaataa
     3241 gttcctcaac attgattggc ttctccgttg tacttatcct ctcctctatc tcatccgatg
     3301 ttttaaatat ctgctttaca tcctgtaaat caatttttcc ggttgcgata ttaaaataga
     3361 ggtcaaccgg taacttaaaa ccataaaatt tgattatttt atttaattcg cgctcattca
     3421 gttcaatttt ccaatttttg aacttcctat agaggatatc ttttccaatt tcggcttctt
     3481 taaatttttg ctccctcaac gcccttgcaa tcctactttt ggcttttgaa gtaacaacaa
     3541 cgtttagcca atcagggttc ggacgttgat tttttgcagt tattatctca accttatcgc
     3601 cgtttttaag ctgatatctg ataggatgaa tttttccatt tatcttaccg cctacacact
     3661 tagaacctac atcagtatga atttcaaaag cgaaatcaag aattgtagcc ccatttttca
     3721 attctctaag atcaccattt ggagtaaaaa tataaatatt gcttgcggca tggtcaattt
     3781 ttgtctgttt ttgttcatca aatcgctgag tgccaatagt ctcgagcaag ttacgtacat
     3841 ccagcaacca attatcaata ttctttccgt aagctgtttc tttatatttc cagtgtgctg
     3901 cactaccctt ttctgcatta acgtccatgc gttcagttct gatttgaact tcaacccatc
     3961 tgtcttccgg ccccaaaaca gttgtatgta acgattcgta accgcttaac ttaggagtag
     4021 taatccaatc tctcaatcgt tttggattag gatcataggt attagcaatt aatgaatagg
     4081 cttgccagca agcggtcttt tcgcggttaa tcagttctaa atatcgtttt tttctatcct
     4141 caaaatcctt gatctcattc tcttgatttt cattaattga ttctaagctt ttttcttttc
     4201 catctttctg ttttttccta ttttccgacg atgaatcttc ctgttcctct ttctctttaa
     4261 tcctttttcg tttaatgcta cgctgatcgc caaatttttc ccaattagct aaataatctt
     4321 ctataaattc atcatcctcg tctgacagca cattttgcaa aataaccctt attgcaaaaa
     4381 gatcataaac cttatcaatt cctatatcct gtgcctccat ttttcttttt atcgaagtga
     4441 tggattttgc acgactttta atttcataac taatacctga cttatcaaat tgctctttga
     4501 taggtctaat aaatttctca atatattttt cctgttgctc ctttgtttcg tgcagtctcg
     4561 attcaatttt actatattcc accgggtagg aatgacgaaa cgccatatct tcaagttgtg
     4621 ctttgatatg gtataaaccc atccggtgag ttatcgggat ataaagatat tgaacgtcat
     4681 tgagaaatat cttcttttga aactccgtca atgaagaata gttgcgcaga tcataaagcc
     4741 ggtgcgctat tttaataaat atcacacgca catcgtcaat catacttaaa aagaggctgc
     4801 ggaagttttc cgaatgaagt tttacctttt ccgaatctat taccgacacc tttttaaacc
     4861 ctcgtacaat ggtggcaaca tcttttccga acttcgattc tatttcctca caattatcat
     4921 cagctgtgtt tattacatca tgcaaaagcg ctgctattaa cgatactgaa cgaagtccaa
     4981 tctcttcggc aacaattctt gcgacagaaa gactatggga tattagaagt tctcccgaag
     5041 aattgatttt atctccataa acctttgcgg cataacgata tgcagcatca agtttgtcca
     5101 actcatccgg agtacaattg gtacaaaatt gttttaacgt ttcaaatttc ttatcaatct
     5161 ctttttcctc aatagttctt atcatcaaaa tcttatgatt ttatgagtta ataaagatta
     5221 attaccttct gatgattttg tttctcctta taaacggagg gagatgctaa gaagtatcaa
     5281 atttttagat cttttaacaa tagtttgatt ctaaaaagaa acacccgtct tctcatagtt
     5341 gaaaagacgg gtgtttatga gttatttttt gaaacagtta cactttttat tttccttcag
     5401 gtttctgttt tgatttagtt tgggtttccg gcgcgccagc tatagaatcc ctcattcggg
     5461 tatcagcctt tatattctca aacttataat aatccataat tcctaaatta ccatttctga
     5521 acgcttctgc aatggctttt ggaatttcag cctctgcctg gataacgttt gcacgggctt
     5581 cctgtgcacg agctttcatc tcttgctcaa gagcaatagc catggagcgt ctttcttccg
     5641 ctttggcctg tgcaatattt ttatcagcat ttgcctgatc catctgcagt actgcgccaa
     5701 tatttttccc tacatcaata tctgcaatat caattgagag aatttcaaaa gctgttcccg
     5761 aatccaatcc tttattgaga acaagcttcg aaatcgtttc gggattttca agcacgcttt
     5821 tatgcgaatt ggccgaaccg atagctgaga caacaccttc gcccaccctg gcaattaccg
     5881 tatcctcacc tgcaccacca accaattgac gaatatttgc ccgcaccgta actcttgctt
     5941 tggcaatcaa ttgaatacca tcttttgcta cagcagcaac tggcggagca ttgataactt
     6001 tgggatttac tgacatttgt actgcttcaa aaacatcacg cccggcgagg tctatcgcag
     6061 tagcagcctt aaaatcgagt cccatatttg ccttatcagc agaaataagc gcattaacaa
     6121 cagatttcaa tctaccaccg gccaaatagt gagcttcaag ttcattcctg tttaatttaa
     6181 gccctgcttt agtggcagct ataaggctat taacaattat agctggcggg attttcctcc
     6241 aacgcatcaa aaccaattgc aacaaagata tccttacacc agatacaagt gccgaaaacc
     6301 acaatccaac cggaatgaaa taaaatatta accaaaggcc aaatagcgaa gctactccaa
     6361 ttgcaataat tattaaaaca ttgctttcca taacattaag tatttaattt tacaaaaatt
     6421 ttctttccct ctatcttcac aactcttatt tcagtctcct gatcaatcag ccccccggta
     6481 gaggttactt caaaatattg attattaata aaggctttac ccatgggtgc aagccttgaa
     6541 atagttttcc ccacatcgcc aacttgaaca gactcttcct ccacaatatt agtcttgcca
     6601 tcaagctcag tatgtaacat aatcctcttc catgtttttg atcgtaatgc taaaattagg
     6661 acaacaactg aaagcaaaac tgttgaaagc aacacgatat tacctcccac tgccccatga
     6721 atagcatagc tttgccaaac gccaattgca atcaatacaa agccaataac accaacaata
     6781 gtggttccgg gtataaccaa cgcttcaaga agcaagaata acaaacccac caaaatcaac
     6841 acaataatga ctgtccacga catattaatg atatttaatt ctattttatc gaaacagtta
     6901 aaccacgatt agtcatctcc gaacttatcg gtatcagctc ctcaacatct cctgacttaa
     6961 ccgaacactt cccattaaaa tgagcaataa gtgtacattg ttcagctgtt aagggatcgt
     7021 gctcgcaaac ttctatcagg gtttctatca caaaatcaaa agtgtttata tcatcattaa
     7081 acagaataag ttcccgtcta tcagtaagtt gctcttcatt ttgatctaat gattttgttc
     7141 gttctttaat catctttttc atctgctttt aggcagcaat aataatgaaa attcaattat
     7201 taaaataaaa agtcagacat tttaccgata atttttcaat aagttatttt tgccgataat
     7261 ggagccaatg caattcgatc aacttattaa gcgccttgaa catagtttaa agcagccttt
     7321 gccgggagta aaagctcata ctcgtttatt tcctcctacc cgttccgaat ttcctcctat
     7381 aaatttttca gctgtaaaaa taggagctgt tcttgctatg cttttttcac aaaatggtca
     7441 aacaagattg acgtttattt tgcggcaaaa atataatggc gtacatagcg ggcaaatctc
     7501 ttttccgggc ggcggttacg aagaaaatga tttaacatat agtaaaacag caaccagaga
     7561 gacacaagag gaaattggcg ttcctgaaga agagataaaa ataatcggtc agttaagcca
     7621 gctttatatc ccaccaagca atttccttgt ttatcctttt gtcggatata tccaaaagca
     7681 accgaattac aaacccgata tcaccgaagt tactgaagtc ttcgatcttt ctctcaacga
     7741 gctaatgaaa gaggaatgtt ttcaatttca gcccgttata tcaagatcaa aacaggtgaa
     7801 agtgccttgc tttgtaactc aaaaccgatt gatttggggc gctactgcaa tgatattaaa
     7861 cgagttactt gaaataataa agcaaggctc tgatttttaa ttcctttaaa gcgccttttc
     7921 ttattaatct aatattttta tctcgcttgt gatctccgat gcttttccca acataaaaga
     7981 tactccacaa taacgttcct gcgagagatt tactgctttt tccaacttat ccattggcaa
     8041 gtcctttccc ctgaactgat actcaatgtg tatttttttg tagtacatcg gatgctcgtt
     8101 tgtggattcg gccaaaacaa caacattaaa atactcaggc tccacccgca tctttcgtaa
     8161 aatggaaatt acatccatac cggtacagcc accaaggccg gcaagcaaca atggctttgg
     8221 acgcggcccc ctgttctctc cacccacttt ttcgtcggca tcaatcaaaa atttatgacc
     8281 attaacttca acgtcaaatg acatacccct gttaagggta acttcagcat atttatccat
     8341 aattaaattt ttcggcaaaa ataactcttt tagtgatact aaagcgattt aattacactt
     8401 tacattttta aacccgatta agtatttgca atcaatactc taataaatct accgaacaaa
     8461 tattagatca ggtttgatta attttggtta aaaaatcaca gttaaagatt tatgaaaaaa
     8521 ttattccatc gaacaacttc atcattaaag aacaaaaagg caatgccatg ctttagtcat
     8581 aaatgaatgg tagtactagt agcgaaatcg tataaaatgc tttcattgat tgtttttttt
     8641 taaggtaaaa ataaatctta ggttgcgctt tcgattttaa attaaaattg tacatttgca
     8701 accgcaaacg gtgatcgtag gtttaaaaaa aactcagatc ataataggga atcacgtgaa
     8761 aatcgtgaac agtacccgct gctgtgagtt ctaaattttt ccggcatccc ttatgccact
     8821 gtttcgcttg aaggaatggg aaggccgccg taaaaggaac aagtcagaag acctaccgtt
     8881 gcttagtaat caaagctttc gggataaaag ctgaggataa aagatgcata tctatttatt
     8941 ccagatgttt tcttgaaagt tttaagattg atcaaactaa tgattatcaa ttgtttaatc
     9001 ttaaaactcg gtattatgaa aagaataatc aggctgtgta aaaccttaag aaaaggagcc
     9061 ttacaggtta atgaacatca cacggaaaat ttaatcccct ttttaacaac aaaatacggg
     9121 catacaagat tcgatggctt tatgttaaca agatattgtt accctgtatg ttataaaagg
     9181 aattatgcgt aaaaaattga taattaccgt actccaattc attaaccgaa taattaattt
     9241 acagtaaaag ctgacttgat atggcgaatg aagtaagatt gaatcattta attatcaaaa
     9301 gcacttttat cgttgttttc atcgtgtttt ggctgacttt accggcacaa aaccataaag
     9361 acacgctaca tcttgaaaca gtagagatag ttgaccaacg acttgaacag gttttaggat
     9421 atcatgaaac caaactaacg aagaggcttg ttacagatcg ggtttcggta aatcttgcag
     9481 attttttgaa cgtaaataca tcaatattta tcaagagtta cgccaatggc gggctggtaa
     9541 caacatcttc cagaggcgca tctgcttcac acacagaggt actatggaat gggattaatc
     9601 taaactcgac gatgaatggg aatgccgata tttcgcaaat acctcttctt tttgtggata
     9661 agataaatgt tggatacggc ggaggcatat ctacttacaa ctcatcagga ttgaccggcg
     9721 cgattgacct tgctacggaa acaaactggc aaaatagatt ttctcttgag ctcaaacagg
     9781 atattagtag ttttaacact tacagaacat attttttatg taacgttggg aattacaaat
     9841 ttcagtcatc caccagattt ttctatgaaa cctccgaaaa caattatcca tttctgaaca
     9901 acaccttaaa tggtgaaaaa cctcaaaaag agtacagaac caatgcatca tttgatcaaa
     9961 cgggattgct tcaggagtta aactggaacc ttgcttcaaa caccaacatt tcattaaagg
    10021 catggataca ggaaaatgac cgcaacctac cctcaaatat tctcaccaag cttcctaatg
    10081 agaaggagag ttcgtatgac caaattgcca gagctgttgt tgaaatgaaa caacaatttt
    10141 ataattcgaa attagaattt acaagtggta tttttcaaag ctggtcaaac tacaaaaaga
    10201 aaaccgcagg aattaatagc gaccacatta cttttacttc ggccaacaag ttaagataca
    10261 agtattccgg ttttaaaaat ttaatgatct taaccgatgt cggatttgat tttcataacg
    10321 taaaatcaga taattacgat agcaagagag agagaggaga gggtaagtta aagctgaatt
    10381 tacaatactt tgggataaaa aagtttgttt tcaacggatc ggtcatccaa aagcttgttg
    10441 atcatactgc tgttccttta atcagctctt ttggcatagg atatcgggtt cgcgaaaaaa
    10501 atccgataat attaaacgca agcgttaccc gtaatataag aatacccagc tttaatgatt
    10561 tatactggac acccggtggc aatccatatt taaaaaatga gaaaggtata attgcagaaa
    10621 ccggtattac aatcgaaaga aactatcgca aaaccaactt aaaaggttca atatctctat
    10681 ttcataacaa aattgatgat tggatcattt ggcaacctga tacgctattt agttactgga
    10741 agccattcaa tttgaaaaaa gggatttcac aaggtattga agctgaatta acagcttccg
    10801 gcgctttgaa ccagcttaaa tggaattatt cacttactta cgccttcacc tcggccaaaa
    10861 acaaaagtcg gacatcaggc tcggataaaa ccataaataa acaattgatt tacgtcccaa
    10921 aacatatagc caaccaatcc atcttagttt cttttaaaag tatgtcagca agttatgtta
    10981 ttagttatac aggcaagcgg tttaccaatc cagatcatta cagttaccta caagcatact
    11041 ttttacaaga ttttgcaatc tcaaaatcat ttgaattggg tggatcaacg ttaaattgtc
    11101 agtttacaat tcacaacatt tttaatcaac aatatcaaat tattgcctgg tatccaatgc
    11161 ctggcaggca tcttagtttt tcaataaaat acaattttaa aaattcaaaa tcatgaacag
    11221 gaaaaaatca acatttgact tttcaagatt agccctcatt tgggctttgt ttcttgcagt
    11281 taacgccatt atgctctatt cttgtaggga tgattacggc gaaattaaaa agaacacctc
    11341 cctttattcc aaagggaaag gtctctttat tgttaatgaa ggaaatttcg gtaatggaaa
    11401 cggttcgatt tcatttttta gtctcgacag cttaaaaatg tataacgata ttttttattc
    11461 agcaaacaac cgaccacttg gcgatgtacc ggtttccatc actttgttgg aagaggagag
    11521 ctggattgtt gttaataatt cgggtaaaat tgaaattgta aaaaattcag atttcagttc
    11581 ggttactact attaccggtt ttacctctcc cagatttctt ttaaaggtaa gcgataaaaa
    11641 agcatacgtc agcgatttat acagcgatca aatcacaatt gtcaacgtga aaacaaaaaa
    11701 gattgaaggt catattccca taaagcgctc atcagagcaa atggtaatgg taggtggaag
    11761 agtattttcg gccttttggt caaactttaa ttttcctgaa tttgaaaaca accagttaat
    11821 gattatagat attgaaaaag atatagtggt tgattcaata caagttggca aagagcccaa
    11881 ctcgatggta ttggacgcca acaacaagat ttgggttctt tgcagtggcg gatacatgtc
    11941 ggaagagact gccactctgc atcgtattaa ccccacaact ttgcaaacag aggtaattca
    12001 tactttttcc gatccatttg catctccaac ttcgctttgc atcaacgcca cgggcgatac
    12061 tcttttatat ttaaatcaag gcatcttcag aatgtcagta acagaggata atctaccgga
    12121 aacggctttt attccgcaaa acagtagatt gttcaacaca ttaggtattg acccgatgac
    12181 atctgaaata tttgttactg acgccattga ttatcagcaa catggattaa tattacattt
    12241 cagtagaaat ggtgaattaa agggaacgta ttcagccgga ataattcccg gaaatctgtg
    12301 tttcaattaa ttggctgctc cgaaaagtaa tttaaccacg aacactttaa ctttgtttag
    12361 taaagtaaat tcgggacttt tagtttgttt aagtgaaaag gattggaaca aaaaaagttt
    12421 tcttttcaaa ccagttttaa aaaaaccaat tattttttcc ttcccggata tacttttaaa
    12481 gcctcttcaa gagccttcat tgcatgttta agatcgtcaa catttaatac atagcttatc
    12541 ctcacttcgt caagtcctcg gccaggtgtc gagtaaaatc ctgttgccgg cgccatcata
    12601 actgtttcgt ttttataatt aaaatcttct aacagccatt ggcaaaactt atccgaattg
    12661 tcaataggca agcgggctac cacataaaat gcacctgacg ggttgggaca gaaagcccct
    12721 ggcattttat taatggcttc ataaactgtt ttacggcgtt tatgatattc ctccaacact
    12781 tcggcaaaat aatcagcttt tgtatctaac gcagcctctg ccgcaatctg gccaaaagtg
    12841 ggcgggctta accgtgcctg agcgaatttc attgcagcgg taagaacctc tttgttttta
    12901 gtaatcatca cccctaccct aaccccacac atgctgtaac gcttcgagac cgaatcaatt
    12961 agaatggtat gctgatctaa tccatctaag tgcataaccg agtggtgcac tttaccatta
    13021 tagcaaaatt cacgatatac ctcatctgca atcaggtaaa gatcatgttt aagtgcaatt
    13081 tcttttaata ccaataactc ctcttttgta taaagatagc ccgtaggatt attaggatta
    13141 caaacaagta tagcctttgt tcgtggagtt atggctttct caaaatcagc aatgggcggc
    13201 agagcaaacc ccgtttcaat atcggaatga ataggtacta catttattcc ggcattgatg
    13261 gcaaagccat tgtaattagc ataaaatggt tctggaataa tcacctcatc ctccggatcc
    13321 ataatagatt gcattgcaaa caatagcgcc tcagaggctc ctgctccaac gataatctga
    13381 tctgctgtga tgtggatatt gttctttttg taatatccca acaacttttc acgatagctt
    13441 gcataacctg ctgaatgact ataagctaca agtttttcct tataattctt aactgcttca
    13501 atagcaatct ccggcgtttt gatatcgggt tgcccgatgt tcaaatgata aaccttaata
    13561 cctctttttt ttgcagcatc cgagtatggc acaagttttc tgatcggaga ggcaggcatt
    13621 tttttccttt ttctgaaatt ttcggcatct caaattttta tttaaaaagt tatacaaaaa
    13681 aaaccggctc gattttagcc ggcattaggt tctacttaaa tcatcttctc gaaaatatta
    13741 tttttcacca acagatttcg gtttttctgg ggcggcgctc tgctgctgcg agagaacctg
    13801 cccggtaatt ttcagaaaca ctgtactgtt agaggcattt gactgaactg ttaccgactt
    13861 attgatagcc cctatcctgt tggttttata ctctacattt attattcctt tatcaccggg
    13921 aaggatcggt tcgcgtggcc actttggcac agtgcatcca caggatgaac gtacattagt
    13981 caaaattaga ggctcctttc ccgtattggt aaactcaaac tcacattttc catcaccgtt
    14041 gtagggtacg gttccgtaat catgaactgt tttttcaaat gttataaccg gagcattggg
    14101 gttttcagca ggctgctctt tcgtctgaag ctcctgagca atcatactga tcgaaaacat
    14161 aattccgagt gaaagcatta aaatcctttt catcgcatta aattttaggt ttaatttgag
    14221 caatttttat tgccacaaaa gtaaataatt tcattgaaat aaattgtcaa aaaaaaatgt
    14281 tcgccatatt gtttattttt ggccgactaa aacttatttg attatgaata aaaaatacat
    14341 tctcctctcc gccattttca ttgcttttct gtttttaagc tactcctgtt gtacatcaag
    14401 aaaccaaaaa aaagcatctt caataactca agagaataca cctcctaagt taccatcgcc
    14461 ggcagtaatt atttataaga cgaaagggga ttacaactac aatgtacctg tgattttgtc
    14521 agttgataag acaagaataa tttcattccc tgatccaaaa gatattcgta ttgatggcaa
    14581 ttttacctat ccggacgagc ttgttgacgg ctacctatta gacaaaagag gcataaacga
    14641 acatgcagca tttttgaagt tcacatacga agattattac aacatggaca acataccaac
    14701 tgctgaacgg ctgatgaact atatccttga tgatgatccg tttattgaaa tttatgatgc
    14761 cggcaaaaaa ggacaatata aaaacatcat agatgattta aacacaatga tacttcaagg
    14821 taagattagg gagatgaaaa acttgttgaa aaaataactt ctaagacaaa aaaataattt
    14881 tttctattag tagcgcttcc ggcaagtttt taatcactgc tgacaaatgg atagatacca
    14941 tttatccctt attgattcaa ggttggcaag aaatgtaaaa tctcaaaaaa ccaatttgaa
    15001 atcgtgattt tccaatattt attccacaac agtattccag ttgtgaatgt cttcctcggt
    15061 tccttcctgg attcctttaa gtttattata taatttcgtg cttatgggtc cagcttttcc
    15121 atctttagag tattctacaa cctttcctgt gtcacggtcc actattttac caattggcgt
    15181 tatcactgct gcggtaccgc aagctcctgc ttcttcgaag ctatcaagtt catccacagc
    15241 aacgcttctc aattcgacat tcaatcctat atcttttgct aattgtctaa ggctcaggtt
    15301 ggtgatcgaa ggaagtattg atggcgagtc tggcgttata taactgttcc ctttaatacc
    15361 aaagaaatta gctgggccag cttcgtcaat atatctacgg tcggctgaat ttcggaaaat
    15421 cacagatggg aagccttcat tttttcctct gatgagagct ttgagacttg cagcatagtt
    15481 tccaccaact ttaatatgtc ctgttccgaa tggcgctgca cggtcataat cgctaatgat
    15541 ctggaagttc accggtttaa aaccctcttt gaaatagggg ccgaccggtc caacaaatac
    15601 cataaacaga tattcgtcgg ctggttttac acctacctct gctccagtac ctattaaaag
    15661 cgggcgtagg taaagggatg caccgtatcc gtaaggagga acatacttct cgtttaactt
    15721 gatagcctta aatatcatct cgccaaatag ttcaaccggc ggtacagcca tcattatacc
    15781 ttcagccgaa cgttgcatcc ttttagcatt ctccatccac ctaaagacac gtatcttccc
    15841 atccgaacca cgaaaagctt tcatcccctc aaaggcttcc tgcccatagt gcaaacatgt
    15901 agctgccata tgcatagtta tatactcgga tgaagagact tctacctcac cccatttacc
    15961 attacgatag tagcaacgta cattataatc agttttaaaa tagccaaaag gcaaattctt
    16021 ccaatcaata tttgtcatcg ttttaaaatt taaagtttaa tgcggctaaa ttataaagaa
    16081 tagagagtta tttaacctta caaaatcaaa taacaagtaa attagaatcg tgttaaataa
    16141 tatgcgaacc ttatatggac atagttgctt gaaaggttgc cactattaat caccgacaaa
    16201 acataagctg gcataggcgc tacaataaat gtaagatgct atttttgcaa gacctctcag
    16261 agaattactt taaaatacta atgctgaaat ttaatattcg tttgaataaa taataaaatg
    16321 atagttaaag aattaattcc acttattgaa ggaaagttgg tgtgtggaga cccggattcc
    16381 tcacatcaaa tgaaacttgc ctttgcctcc gatctgatga gcgatgtatt aacacttgac
    16441 agcaacaatc cattgttgat tacc
//
LOCUS       DAOOAI010000046        16367 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_4625_length_16367_cov_5.454022, whole genome shotgun sequence.
ACCESSION   DAOOAI010000046 DAOOAI010000000
VERSION     DAOOAI010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16367)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16367)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16367
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4625_length_16367_cov_5.454022"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..613
                     /locus_tag="PK345_04770"
     CDS             38..613
                     /locus_tag="PK345_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013619229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPX34364.1"
                     /translation="MKKAVQEINAGSMADIAFLLLIFFLVTTTMDVDTGIGRRLPPPL
                     TGDEKPPDIKERNIFTVLINNQDRLSVEGRPSDIRLLKQQAKEFLSNPHNSEHQPEMV
                     MKNIPELGLVRVPKSAVISLKNDRGTTYKMYIAVQNELTAAVAELRNELSMTKFGRKF
                     TELTNEAQIEAIQTAIPIPISEAEPVEVGGL"
     gene            616..1098
                     /locus_tag="PK345_04775"
     CDS             616..1098
                     /locus_tag="PK345_04775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HPX34365.1"
                     /translation="MARFKKRKKYGVPALSTASLPDVIFMLLFFFMVTTVMREVTLIV
                     KVKPPEASEVQRLEKKSLVSYIYIGEPIKSNLFGTKPRIQLNDQFGRVEDIPAFITSE
                     RQKRDEFDRRFLTTSLKVDQDISMGTVTDVKQELRKSGAFRINYSVRKVEKMNFGKLL
                     "
     gene            complement(1152..2912)
                     /gene="aspS"
                     /locus_tag="PK345_04780"
     CDS             complement(1152..2912)
                     /gene="aspS"
                     /locus_tag="PK345_04780"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019537930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HPX34366.1"
                     /translation="MYRTHTCGELTLNSVGEYVTLCGWVQRVRDKGGLIWIDLRDRYG
                     ITQLILEEGKSNQEIIDKAKKLGREFVVKVSGNVIERASKNPKMTTGDIEIFVDTIEI
                     VNQSKLPPFTIEDQTDGGDELRMKYRYLDLRRTPVKNNLMLRHRIAIETRNYLDKQNF
                     IEVETPVLMKSTPEGARDFVVPSRMSPGEFYALPQSPQTFKQLLMVAGFDRYYQIVKC
                     FRDEDLRADRQPEFTQIDCEMSFVDQDDILNTFEGLIKYLFKTVKQIELAEIFPRMTF
                     DDAIRFYGSDKPDIRFGMTFVELNDLVRGFGFKVFDEAELIVGINVHGCADYTRKQID
                     QLTEFVKKPQIGASGLIYVRYNNDETVKSSVDKFFNEEQLKKWVHLFNSKPGDLILIL
                     AGASLLTRKALGSLRLEMADWLGLRNPDVFAPLWVVDFPLLEWGEEEMRYFAMHHPFT
                     SPKLNDIDLLDTDPSKVHANAYDLVINGVEIGGGSIRIFDKLLQQKMFQVLGFTQEEA
                     QNQFGFLMNAFEYGAPPHGGIALGFDRLVSLFGGSDSIRDFIAFPKNNAGRDVMIDAP
                     SQIAKKQLDELGLIVVQKGK"
     gene            3122..3802
                     /locus_tag="PK345_04785"
     CDS             3122..3802
                     /locus_tag="PK345_04785"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPX34367.1"
                     /translation="MKKIILCTIFIVTVIFGFSQSADKTNQNNFSSVIDVFNDIYLNV
                     PDSVKERFFNPGISFSGFFEHHFKNSNFSVAIGAGIGSHNFYSNSFVVADTNGITGLR
                     PIKNIYPNNSYKKNKISYTYLDFPVEVRMRAKNDIRAAIGFKVGVLLDSHSKYKGDDY
                     LSGTKKSLFIKTKDINDIEKLRYGVSTRFGWKFINVTGFYSLSNLFAKDLGPELYPIS
                     FGISLMPF"
     gene            complement(3799..4401)
                     /locus_tag="PK345_04790"
     CDS             complement(3799..4401)
                     /locus_tag="PK345_04790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34368.1"
                     /translation="MKFPRIISSFLNPFFITFYFFIITLNLNTKAINPLPEKAKWIIY
                     GLIIIITYVIPGLFIKFYGSFIQRFFKYGQKEKRIIYFFIVTVFYYLTYYLLIQLRLS
                     PIYSLFLFGCTSLLVLCLIVSFFWNISIYMVAIGALSGGLLGVSMVFNINLLFPIFMV
                     ILFSGLLGYSRLSLGKHRPLEVYGGYLTGLAIMLAHYFLF"
     gene            complement(4443..5921)
                     /gene="rpoN"
                     /locus_tag="PK345_04795"
     CDS             complement(4443..5921)
                     /gene="rpoN"
                     /locus_tag="PK345_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005860047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HPX34369.1"
                     /translation="MGKQGLYQKLSIQQKLSPQQILLMKMLQIPAIALEQRIKQEIEE
                     NPALDELTGYDDNGGEAEKDEDEFINELDNSESDEFDLKDFIGDDDTPGYKLRANNYS
                     RDDDNKKEIPFSVGKSFHDHLTSQLGLKRLNEKQLAIATTIIGNMDDAGYLRREISSM
                     VDDLSFSQNITTTEGEILKVLKIIHELEPAGVGARDLRECLLIQIRRWQAEEPETNFD
                     LSILILEKYFEEFSKKHYDKIIKRARVTEDELREAIEQILKLNPKPGNSLGETTRTSQ
                     YILPDFIITTNNGELELSLHSKNAPELRINRQYLEMIEGYMLDKSKKTRQQRETFQFI
                     KSKIESAKWFIDAIRQRQNTLLLTMNAIMEYQKEYFQTGDETKLRPMILKDIAEIVNL
                     DISTVSRVANSKYVQTPFGTFLLKTFFSESMQTKTGEEVSTREIKKILQDTIDEEDKT
                     KPVTDDQLTAILKQKGYSIARRTVAKYREQLNIPVSRLRKEL"
     gene            complement(6000..7391)
                     /gene="asnS"
                     /locus_tag="PK345_04800"
     CDS             complement(6000..7391)
                     /gene="asnS"
                     /locus_tag="PK345_04800"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004816 - asparagine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006421 - asparaginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HPX34370.1"
                     /translation="MIKQSKIVDLLKSKDYNREVNVKGWVRTKRESASVAFIALNDGS
                     IIHHIQIVIDMKQFDAELIKKTTTGSGISVTGILVKSLGKGQSVEIQAKEIVLYGTAD
                     PETYPLQKKGHSLEFLREIAHLRPRTNTFGAIFRIRHALAYAIHKYFNDRGFCYLHSP
                     IITSSDAEGAGAMFRVTTLDAKNPPLLPDGSVNFKEDFFGKATNLTVSGQLEAELGAL
                     ALSLVYTFGPTFRAENSNTPRHLAEFWMIEPEMAFYEIKDNMDLAEDFLKYLVKYVMQ
                     NCSEDLEFLNNMYDKELIERLKSIIEVDYERLSYTQAVEILKTSGKKFEFPVHWGVDL
                     QAEHERYLVEQHFKKPVILTDYPKEIKAFYMKQNDDGKTVRAMDVLFPKIGEIIGGSQ
                     REESLDKLVQRCKEMNIPEKDIWWYIDTRKFGTVPHSGFGLGFERMVLFITGMKNIRD
                     VIPFPRTPQNAEF"
     gene            7616..8149
                     /locus_tag="PK345_04805"
     CDS             7616..8149
                     /locus_tag="PK345_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPX34371.1"
                     /translation="MQKRKISNSELNRLTPEEFRKANKNPIIIVLDNMRSHNNIGSIF
                     RTADAFLIEAIYLCGITACPPHRDIHKTALGATETVEWKYFEKTETAIEELKSAGYII
                     YAVEQVENATNLDKLNINKETKIALVVGHEINGVSQQIVNQSDLCIEIPQLGTKHSLN
                     VAVSVGIVVWHLLFLLK"
     gene            8336..10237
                     /locus_tag="PK345_04810"
     CDS             8336..10237
                     /locus_tag="PK345_04810"
                     /inference="COORDINATES: protein motif:HMM:NF024579.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34372.1"
                     /translation="MSKKQTLAKFAAILLIAVMIVGCNSLNKMAKQFNSITYEVTPQV
                     LESKGGIVTFTVKANLPAKFFNKKAGVVIQPTIEYEGGKVLLRPIVLKGENVSGEGTT
                     ITYNNGGSFTYTETFGFTDEMKEAELVINSIAFLPKKPIEAGITLEDARMMRKSFIFD
                     EKKLADGIIHTSHRINVENEVREMVEVGDENPILDANGNIDLKAYQVKDGQKVEMLRL
                     APHGYEKVTLASENATIYFAKNLHDWNANLEWNKKEDVKAQLDKVYNFVRQGWEIKDV
                     VINGWASPEGEETFNDGLSEKRAKTAEGILKRSFDNVAKEKETKVNFKSSTEINFKNI
                     GNGPDWNGFTKRVEASEVKDKQQILNVTKSSKPEQREEEIRNMILIFPELEKSILPAQ
                     RRAEIVVTCYEPKKTDEEIARLATTDPSELTQAELLYAGTLTTEWETIYNIYKSAANM
                     FSNSWEAQNNAGYIALKMGKVEEAKDFFQKAEKLNANNGIITNNLGVIYAYQGNFKQA
                     ETYFNEANKMGIDNNYNLGIIDISKADYKGAISKFAGVSCNYNVALANMIDGNNGAAT
                     TNLDCARKNAATYYLMAIAGARTDNQEMMLSNLKKAIKANKKFKEEAKIDREFIKYFN
                     NAEFKDIVK"
     gene            complement(10381..14196)
                     /locus_tag="PK345_04815"
     CDS             complement(10381..14196)
                     /locus_tag="PK345_04815"
                     /inference="COORDINATES: protein motif:HMM:NF014850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2 TIM barrel-domain
                     containing protein"
                     /protein_id="HPX34373.1"
                     /translation="MKIFTTSLFFTFLFFFAVPAFTQHNNIFNDIENPEVVSRNKLPA
                     RSFAIPYSSVNQAIDNIWDSSPYFMSLNGKWKFKYSDRLDQRPDGFYLTEYDFSDWDE
                     ISVPSNWEIQGYGIPIYVNQPYEWTYDPQPPTIPHIENPSGSYITFFEVPENWLGGKI
                     ILHFGGAKSALYVWINGEEVGYSQDGKLPAEFDITPYIRKGKNKLAAQVIRWSDGSYL
                     ECQDFWRISGIQRDVFLYSVPLVNISDFEVNAGLVNEYRDGQLSVLLKIDNDNKKKTP
                     KLNSTIHLFGSSADAVIYQESKTIKLAAKESGEIKFNVFIQNPRKWSAENPELYKLVI
                     TLSDKKGNILQAFSSNVGFRTSEIKNGQLLINGTAVTLKGVNRHEHDPLTGHVISEEM
                     MKEDIFLMKKNNINAVRTAHYPNDPRWYDLCDRYGLYVVDEANIESHGMGYGDRSLAK
                     DPLWGTAHLDRVKKMVERDKNHPSIIIWSLGNEAGDGVNFSACYQWVKFRDPSRPVQY
                     ERALLGPNTDIYCPMYASVEHIEDYVRKHQDRPLIMCEYAHAMGNSSGNLKEYWELID
                     RYNQLQGGFVWDWVDQGLLTKASDGSQFYAYGGDFGPEGTPSDGNFCINGLVSPDRTP
                     HPGLFEVKKVYQNIDVKTINPLAGKFHIINKYDFTSLKHYYLIWEIKSEGKQILNGVV
                     DRLPVGAHESFTLNLDFSSLSFKPEKEYFIHFSFLTKASDEIIPANFEVAAEQIEIPN
                     YEPSLDRPIRNIPELKVNESSNLLVFEGRNFQISFDRESGELSNWLIDGKAVINEPLK
                     ANFWRAPTDNDFGNGMDKRCAPWKEASEFSQDMTSRFKILNKSEAIFSTNCILPSVNA
                     QLKMEYNINGLGEIALSTSLTMINPPRPDVEVLVNSKTEFEKAIDFNAMTAHLQLNDP
                     GFIELPEFTLETLIYPTSFSEMNMIWTNKNWSRGKLHYEFRENGKLYAFIGGNINKAF
                     GYPFKTHHWYMISLVYSSFDKKLDLYVNGEPVESIELDHAMTVNISGDSYLGGNPYGE
                     RLFHGRMDEFRLWNRKLTLEEIRNGYLSELSEIKDGLLLYFNFNEMVGNQIKAKVGKK
                     MILTYNDLRSVRPEMPRFGIRFAMPANYENLKWFGRGPHENYCDRNSSAFVDLYQSKV
                     EDQYFPYIRPQENGYKTDSRWLTLTDETGNGLLISGAPLFSFSALNNPIEDFDQGKKE
                     NYRHTNDIISRDKVFVTVDLMQMGVGGDNSWGAMPHPQYLLPAGDYVFKCKLIPFKKS
                     DNPFLFTGK"
     gene            complement(14285..14977)
                     /locus_tag="PK345_04820"
     CDS             complement(14285..14977)
                     /locus_tag="PK345_04820"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX34374.1"
                     /translation="MTKAKILYVEDDQTLSFVTRDNLESHGYAIDFCEDGEQAMKLYN
                     ANHYDLCILDVMLPKMDGFEVAASIREKDVDIPIIFLTAKSTKEDKIHGLRLGGDDYI
                     TKPFSIEELILKIEVFLKRSGQPRLESIEVHNIGEYTFSVKNQRLTFNNKIKELTQRE
                     SELLCMFCENRDTIVKRDDLLMKVWGDDHYFASRSLDVFVSRLRKYLREDPKVKIENI
                     HNVGYRLIIQSK"
     gene            complement(14980..16245)
                     /locus_tag="PK345_04825"
     CDS             complement(14980..16245)
                     /locus_tag="PK345_04825"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HPX34375.1"
                     /translation="MTRNKIRFIVILGAFAITSIIVVQVYWLRNTWNIKEKQFSQTVH
                     IALFEVAEKMSRYNQSVLPATNPVNQLSASYFVVNLNSVIDANILEYYLKTEFSIHNI
                     ITDYEYAIYDCHDDKMVYGKYVSAKGENKPINMSVDLPKYSDYIYYFGINFPSMRNYL
                     GADMTLWFIFTAILFVSVIFFVYSIFIILQQKRYSELQKDFINNMTHEFKTPISSINI
                     SADVLASPNIIEQPQRLKSYSAIIKQENQRLNNLVEKVLQIAHIEKGGYELKKEAIQL
                     NNLITEVVENHQRQCANGRSITFTVQLSPEIKEVLADKLHLTNIIYNLLDNAVKYSGV
                     VPQVTVTTSMNRDKIRLIVSDNGPGIDTEYQKKIFQKFFRIPTGNVHNVKGFGLGLYY
                     VLKVCEAHGWKLYLESEVGKGSDFIIEMI"
BASE COUNT         5273 a         3221 c         2803 g         5070 t
ORIGIN      
        1 aagtatatca agtgttttta aataaatttt aaaaattatg aaaaaggcag ttcaagagat
       61 caatgccggc tcaatggcag acattgcctt cctcttgctg attttcttcc ttgttaccac
      121 aacaatggat gtggatacag gaattgggag aaggctacca ccgccattga ctggtgatga
      181 aaaaccaccc gacatcaaag agcgcaatat ttttaccgtg ttgatcaata atcaagacag
      241 gctgtctgta gaagggagac caagcgatat ccgattgctt aaacaacaag ctaaggaatt
      301 cctgtcgaac ccccacaaca gtgaacatca gcctgaaatg gtaatgaaaa atattccgga
      361 acttgggttg gttagagtgc caaaaagcgc ggtcatctct ttaaaaaacg acagaggaac
      421 cacatataaa atgtacatag cagtacaaaa cgaactaaca gccgctgttg ctgaacttcg
      481 aaatgagctt tcgatgacaa aatttggaag aaaatttacc gaattaacta acgaagcaca
      541 gattgaagcc atacaaacgg ctatacctat tcctatttct gaagctgaac ccgttgaagt
      601 tggaggacta tagccatggc aagatttaaa aaaagaaaaa aatacggagt accagctctc
      661 agcacagcat cattacctga tgtcattttt atgctgttgt ttttcttcat ggtaactacc
      721 gtaatgcgtg aggtaaccct tatcgtcaaa gtgaaaccac cggaagcttc cgaagtacaa
      781 cgacttgaaa aaaaatcatt ggtaagctat atatacatcg gcgaaccaat aaaatcaaac
      841 cttttcggta ctaaaccacg aattcagcta aacgatcagt ttggccgagt tgaggatata
      901 cccgctttta tcacctccga acgtcaaaaa cgcgatgagt ttgataggag gtttttaaca
      961 acttcactaa aagttgatca ggatataagc atgggcaccg taaccgatgt aaaacaggag
     1021 cttagaaaat caggagcatt tagaatcaac tattcagtaa gaaaagttga aaaaatgaat
     1081 ttcggaaaac tactttaata tttttaaata aaatgttatt aaaaggtggc caataacttg
     1141 accacctttt tttatttgcc tttttgtacg acaatcaacc ctaactcatc aagttgtttt
     1201 tttgcaattt gtgaaggtgc atcaatcatt acatcgcgtc ctgcattatt ttttgggaaa
     1261 gcaataaaat cgcggattga atcagaaccg ccaaagagcg atactaaacg atcaaatcct
     1321 aaagctatcc ctccatgtgg tggcgctcca tattcgaaag cattcattag aaatccaaat
     1381 tgattctgag cctcctcctg tgtaaaacca agaacttgaa acattttttg ctgtaacagt
     1441 ttatcaaaaa tcctaataga tccgccgcca atctccactc cattaataac caaatcataa
     1501 gcattagcat gtaccttcga aggatctgta tcgagcagat caatatcatt aagttttgga
     1561 gaagtaaagg gatggtgcat ggcaaaataa cgcatctcct cctcacccca ttcaagtaat
     1621 ggaaagtcaa caacccataa tggcgcaaaa acatcggggt tacgtagtcc gagccagtct
     1681 gccatttcga gacgaaggct acctaacgct tttcgggtta aaagtgatgc tcctgccaaa
     1741 ataagaatca aatcacctgg tttggaatta aatagatgaa cccatttttt tagttgctct
     1801 tcgttaaaaa acttatctac tgatgatttt accgtttcgt cattattgta tcgtacatat
     1861 atcaatcctg acgcaccaat ttgaggcttt tttacaaatt cggtaagttg gtcaatttgt
     1921 ttacgggtat aatcagcaca tccatgaaca ttaattccaa caataagctc ggcctcatca
     1981 aacaccttga aaccaaagcc acgaaccaaa tcgtttaatt caacgaaagt cattccgaaa
     2041 cgaatatccg gcttgtcgct tccataaaat ctaatggcat catcaaaggt catccgagga
     2101 aatatttcgg ctaactcaat ctgcttaacc gttttaaaga ggtattttat cagtccttca
     2161 aaagtgttca gaatatcatc ttgatctacg aatgacatct cgcaatcaat ttgtgtaaac
     2221 tcaggctgac gatcggctct caggtcttcg tcgcgaaaac atttaacaat ctgataatag
     2281 cggtcgaaac cggccaccat gagcaattgt ttgaatgtct gcggggactg tggaagggca
     2341 taaaactcgc cagggctcat tcgtgacggt acaacaaaat ctcttgcacc ctcaggggtt
     2401 gatttcatca acacaggtgt ctctacctcg ataaagtttt gtttgtcaag atagttacgt
     2461 gtttcgatag cgatcctgtg gcgcaacatc agattatttt ttacaggtgt acgacgcaaa
     2521 tccaggtatc tatatttcat ccgaagctca tcaccaccat ccgtttgatc ttcaatagta
     2581 aaaggtggaa gttttgactg gtttactatt tctatcgtat caacaaatat ttcaatatct
     2641 ccggtggtca tctttggatt ttttgaggca cgttcaataa cattgcctga tactttaaca
     2701 acaaattcgc gcccaagttt ttttgcctta tctataatct cctgattcga ttttccctct
     2761 tcaagtatca attgtgtaat accataacga tcgcgtaaat caatccatat caagcctcct
     2821 ttatctctta cacgctgtac ccatccgcac agggtaacat attcccctac cgaattcaga
     2881 gttaactcac cacaggtatg tgttctgtac ataataaatt gatgttttaa aaattttgcc
     2941 gacgaaagta taaaaaatgg gcattggtta gtttatagtt ttatcacttt acaattatta
     3001 attcataatg ttacgttttt gcacactcta aatcatagta ttgtttttaa ccccttacta
     3061 ttagcataat aattgtgagc taacaaaaat taaccctact tttgcacaaa actttcaagt
     3121 catgaaaaaa ataattcttt gtaccatctt catagtaacg gtaatttttg gcttctcgca
     3181 aagcgctgac aaaaccaatc aaaacaattt cagtagtgta attgatgttt tcaacgatat
     3241 ctatttaaat gtacctgact ctgtaaaaga acgatttttt aatcccggga ttagcttctc
     3301 cggctttttc gaacaccatt ttaaaaatag taattttagc gttgctattg gcgccggcat
     3361 cggaagccat aacttttaca gcaatagttt tgtagttgcc gatactaatg gaattaccgg
     3421 tttaagacca ataaaaaaca tctatcccaa caattcgtat aaaaaaaata agatatcgta
     3481 cacttatctt gattttccag tagaagtaag gatgagagca aaaaatgata tccgcgctgc
     3541 aataggtttt aaagtcggag tattattgga ttctcattct aaatataaag gggatgatta
     3601 tctttccgga accaaaaaat cactttttat taaaacaaag gacatcaatg atatagaaaa
     3661 actaagatat ggtgtttcaa caagatttgg atggaaattt attaacgtaa cagggtttta
     3721 ttctctaagc aacctatttg ccaaagattt aggcccagag ctttatccca tttcgtttgg
     3781 aatatctctt atgccttttt agaataaaaa gtaatgtgca agcattattg ccaatcctgt
     3841 taaatagcct ccataaacct ctaacgggcg atgtttgcct aacgaaagcc tcgaataacc
     3901 aaggaggcct gaaaaaagaa ttaccataaa aattggaaaa aggagattga tgttaaaaac
     3961 catcgaaaca cctaataatc ctcccgataa agcgcctatg gctaccatat aaatgctaat
     4021 attccaaaag aatgaaacga taagacaaag tactaacaga gatgtgcaac caaaaagaaa
     4081 caagctataa atcggcgata accttagctg gattaatagg taataggtga ggtaataaaa
     4141 gactgtaaca ataaaaaagt agataatcct tttctctttt tgtccgtatt tgaaaaaccg
     4201 ttgaataaag ctaccgtaga acttgatgaa tagaccagga attacatatg ttataatgat
     4261 gattaagccg taaattatcc atttagcttt ttcgggcaat ggatttattg ccttagtatt
     4321 caaattaagg gttattataa aaaaatagaa ggtgatgaaa aacggattaa gaaaacttga
     4381 gattatcctt ggaaacttca tttttaagtg attagaaatc aggatttagt gtttttaaat
     4441 cgtcataatt ccttgcgcag cctggagacg ggtatgttta gctgttccct atatttagca
     4501 acggttctac gcgcgatgct gtaacctttc tgttttaata ttgctgtaag ttgatcatcg
     4561 gtaaccggtt ttgttttatc ctcttcgtca attgtgtcct gtaatatttt tttgatctcc
     4621 cttgttgata cctcttcgcc tgttttagtc tgcatagatt ccgaaaaaaa agtctttaac
     4681 aagaaagttc cgaaaggcgt ttgaacatat ttgctattgg caactctcga gactgttgaa
     4741 atatcaagat ttactatttc ggcaatgtct ttaagtatca taggtcttaa ttttgtttca
     4801 tcgcctgttt gaaaatactc tttctgatac tccattattg cattcatagt gagtagcaat
     4861 gtattctggc gttgcctaat tgcatcaata aaccatttgg cagactctat tttactttta
     4921 ataaactgaa aagtctccct ttgttgtctt gttttttttg atttatcaag catatatccc
     4981 tcaatcattt caagatattg ccgattgatt ctcaattccg gggcattttt cgaatgtagc
     5041 gacaattcta attcaccatt attagttgtg attataaaat cagggagtat atattggctt
     5101 gtgcgtgttg tttcacccaa cgaatttcca ggtttgggat ttaattttaa tatctgttca
     5161 attgcctctc ttaactcatc ttcagttacc cgagctcttt taatgatttt atcataatgt
     5221 tttttagaaa actcttcaaa atacttttcc aagattaaga tactcagatc aaaattcgtt
     5281 tcaggctctt ctgcctgcca ccgtcgtatc tggatcaata gacattctct gaggtcgcgt
     5341 gcgccaactc ctgccggttc gagctcatgt attattttaa gcactttcag tatttctcct
     5401 tcggttgtag tgatattttg cgaaaaagag aggtcgtcaa ccattgagct aatttcgcgt
     5461 cgcaaataac cagcatcatc catatttccg ataatggttg tagctattgc gagttgcttt
     5521 tcgttcaatc gtttaagtcc gagttgagat gtcaaatgat catgaaaact ttttccaact
     5581 gaaaaaggaa tctctttttt attatcatca tcccggctat agttatttgc ccgaagctta
     5641 taaccaggcg tatcgtcatc tccaataaaa tcttttaaat caaattcatc actttcgctg
     5701 ttatccaatt cattaatgaa ttcatcctca tctttttcag cctctcctcc attatcatcg
     5761 taacctgtca attcatcaag agccggattt tcttcaatct cttgttttat acgttgttca
     5821 agtgcaatgg cagggatttg caacatcttc atcaaaagaa tctgttgcgg ggaaagcttt
     5881 tgctgtattg acaacttctg atataatccc tgtttaccca tgaatgatta attgaaaaat
     5941 attagcaaag ttaacaaatt cagtaattcg agaaaggata aaaggcgttt tcaggtaatt
     6001 taaaattcag cattttgagg tgttcttggg aaaggtatca catctcgaat atttttcatt
     6061 cctgtgataa acaaaaccat ccgctcaaat cctaatccaa atccgctatg gggtactgta
     6121 ccgaattttc gtgtgtcaat ataccaccag atgtcctttt cggggatatt catttcttta
     6181 cagcgctgca ccaacttatc caaactctct tctctttgtg atcccccaat gatctcacct
     6241 attttaggaa ataatacatc catagcacga acggtttttc catcatcatt ttgtttcatg
     6301 taaaatgctt ttatctcttt gggataatca gttaagatga cgggtttttt aaagtgctgt
     6361 tcaaccaaat atctctcgtg ctcagcctga agatcaacac cccaatgaac tggaaactca
     6421 aactttttcc cagaggtttt taatatttca acagcctgag tataactgag gcgttcgtaa
     6481 tctacttcaa taatagattt caaccgttct atcaactctt tgtcgtacat attattgagg
     6541 aactccaaat cttcagaaca gttttgcata acatacttaa ccaagtattt caagaaatcc
     6601 tcagcaagat ccatattatc tttaatttca taaaaggcca tttccggctc tatcatccaa
     6661 aattcggcta agtgccttgg tgtgtttgaa ttttcggctc tgaatgtagg gccaaaagta
     6721 taaacaagtg atagtgcaag cgctcctaac tcggcctcca actgccctga aactgtgagg
     6781 ttggtagcct taccaaaaaa atcttcttta aaatttacac taccatcagg taacaaaggt
     6841 ggatttttgg catccaacgt agtaacccta aacattgcac cggcgccctc ggcatccgaa
     6901 cttgtgataa tgggtgaatg aagataacaa aacccacgat cgttgaaata tttatgaatt
     6961 gcataggcaa gcgcatgacg tattctaaaa atagccccaa aggtattggt acgtggacgc
     7021 aagtgggcaa tttcgcgtaa aaattctaaa ctatgtcctt tcttttgcaa agggtaagtc
     7081 tccggatcgg cagtgccata taaaactatc tctttagctt gaatctctac tgactgcccc
     7141 ttcccgaggg attttaccaa aattccggta acagatattc ccgagccggt ggttgtcttt
     7201 tttattagtt cagcatcaaa ttgcttcatg tcaataacaa tttgtatatg atgaatgatt
     7261 gatccatcat tcaatgcaat gaaagcaaca gaagcgcttt ctcttttagt tcttacccag
     7321 cctttaacat tcacctcacg gttatagtct ttgctcttga gcaggtcaac aattttcgac
     7381 tgtttgatca tttttaataa attttttacg gctgcaaaaa taaccacttt ctgacggtta
     7441 aaacacagaa aatcattttt acctgtaaaa acatccacca tttcgatatt ccaaactcaa
     7501 aggagacaat gattatagca tcttttatat ccattaaagc caaattagac attctcaaac
     7561 acttaatttg gttttaaatt atttttgccg acgcagtatc aaaaaaatac cgctaatgca
     7621 aaagagaaaa atcagtaata gcgagttaaa ccgattaacg cctgaggaat ttagaaaggc
     7681 taataaaaac ccaattatca ttgtattgga caacatgcgt agccataata atattggatc
     7741 tattttccgc actgccgatg ctttcctcat tgaggctatt tacttatgtg ggataaccgc
     7801 ttgtccgccc caccgcgaca ttcacaaaac agcccttggc gcaacagaaa cagttgaatg
     7861 gaaatatttt gaaaaaaccg aaacagccat tgaagaatta aaatcggcag gttatatcat
     7921 ttatgccgtt gagcaagtag aaaacgccac taatctggat aaactcaaca ttaacaaaga
     7981 gacaaaaata gcgctcgtag tagggcacga aataaatggt gtatcacaac aaatagtaaa
     8041 tcaatccgat ttatgcatcg aaatcccaca attgggcact aaacattccc tcaatgttgc
     8101 cgtttcggtt ggaattgttg tttggcattt acttttttta ttaaaataag tgtataaaac
     8161 aatcacattc tcaaacaaaa tagtttgtta atcacaagta atttatattt ttgcagaaaa
     8221 tatcagggat aaaatctcat taataaataa aaagtagtct attttataaa tcctgatttt
     8281 ttagaaacta attatttaaa aataactcta ttactaacta aaaaaaatta gaataatgag
     8341 taaaaaacag actttagcca agtttgcagc aattttgcta atcgctgtaa tgatcgttgg
     8401 atgtaacagc cttaacaaaa tggccaagca atttaattca atcacttacg aagtaactcc
     8461 gcaagttctt gaatcaaagg gtggtattgt tacttttaca gtaaaagcca atcttccagc
     8521 caagtttttc aacaaaaaag ccggtgttgt aattcaacct acaattgaat atgaaggcgg
     8581 gaaagtgtta ttacggccaa tagttttaaa aggtgaaaac gtttcgggcg aaggtactac
     8641 cattacctat aataatggcg gctcctttac ctataccgaa actttcggtt ttacggatga
     8701 aatgaaagaa gcagaactgg tgataaatag cattgcattt ttaccaaaaa aaccgattga
     8761 agcaggaatt actcttgagg acgcacgcat gatgaggaaa tctttcattt ttgatgaaaa
     8821 gaaacttgcc gatgggatta tccacacctc gcatcgtatt aatgtagaaa atgaagtacg
     8881 tgaaatggtt gaggtaggtg atgaaaaccc aatactcgat gccaatggta atattgacct
     8941 gaaagcatat caggtaaaag atggtcaaaa ggttgaaatg ctccgtcttg cccctcacgg
     9001 gtacgaaaaa gttaccctcg ccagcgaaaa cgcaacaatc tattttgcta aaaatcttca
     9061 cgactggaat gcaaaccttg aatggaataa gaaagaagat gtaaaagcac aattagataa
     9121 ggtttacaat ttcgtacgtc aagggtggga aataaaagat gtagtgatca atggatgggc
     9181 atcgccagaa ggtgaagaga cctttaatga tggattgtca gaaaaacgtg ctaaaaccgc
     9241 tgagggtatc ctaaaaagaa gttttgacaa tgttgctaaa gaaaaagaaa ccaaagtaaa
     9301 tttcaaatct tcgacagaga taaactttaa aaatattgga aatggacctg attggaatgg
     9361 ttttacaaag agagttgagg cttctgaagt aaaagacaaa cagcagattc ttaacgttac
     9421 aaaatcctca aagccagaac agcgcgaaga ggagatcagg aatatgattc tcattttccc
     9481 cgaactggaa aagagcatct taccagctca acgccgtgca gaaatcgtgg ttacctgtta
     9541 cgaaccaaag aaaaccgatg aagaaattgc acgtcttgca actactgatc catcagaact
     9601 tacacaggct gaattacttt atgcaggaac actcacaaca gaatgggaaa ccatttacaa
     9661 tatttataaa tctgccgcta atatgttctc aaatagctgg gaagcacaaa acaatgccgg
     9721 atacatcgca ttgaaaatgg gtaaagtaga agaggcaaaa gattttttcc aaaaagcaga
     9781 aaaactaaat gctaacaatg gaataataac caataacctt ggtgtgatat atgcctatca
     9841 gggtaacttt aaacaagcag agacatactt caacgaagct aacaaaatgg gtattgacaa
     9901 caactacaac cttggcatta ttgatatttc aaaagctgac tacaaaggcg ctatcagtaa
     9961 atttgccggt gtaagctgta actataatgt ggctttagcc aatatgattg atggcaacaa
    10021 cggagctgct acaaccaatc ttgattgtgc cagaaaaaat gctgcaacat attatttgat
    10081 ggcaattgca ggagcacgta ctgataatca ggaaatgatg ttgagcaacc tgaagaaagc
    10141 tattaaagcc aataaaaagt ttaaagagga agccaaaatt gatagggagt ttatcaaata
    10201 ctttaataac gccgaattta aagatattgt aaagtaaaaa aacttaacct ttaagaaaaa
    10261 aacccgggat ttctcctggg ttttttttat cctacatatc tcacaaattt aaagcaaatc
    10321 caaaaataat caataattca ggttaagcct gaatattgag aaaaacctta ataagcacat
    10381 ctactttcca gtgaacaaaa atgggttatc cgattttttg aatggaatca atttgcattt
    10441 aaatacataa tcaccggccg gtaacaaata ctgaggatgg ggcatcgccc cccagctatt
    10501 gtcgccacca acacccatct gcatcagatc aacagtaaca aaaactttgt ctcttgaaat
    10561 aatatcgttt gtatgtctgt aattctcttt ctttccttga tcaaaatctt caatagggtt
    10621 gtttaaagcc gagaaagaaa aaagaggtgc gccacttatc aataagccat ttcctgtttc
    10681 atcagtaagc gtcaaccagc ggctatcagt tttataaccg ttttcctgtg ggcgtatgta
    10741 aggaaaatat tgatcttcaa ctttactttg atatagatca acaaaggccg atgaattacg
    10801 gtcgcaataa ttttcgtgag ggcctctgcc aaaccacttt aagttttcat aatttgcagg
    10861 catcgcaaat cttatcccaa aacgcggcat ctcaggccta acagatctta aatcattgta
    10921 ggtaagtatc attttcttac caactttggc tttaatctga ttgccgacca tctcattgaa
    10981 attgaaatag agcaataacc catctttgat ttccgaaagt tcggataaat agccatttct
    11041 aatctcctct aaagtaagtt tacgattcca aagccggaat tcgtccatac gaccatgaaa
    11101 caatctttca ccataagggt tgcctcctaa gtaactatct ccagatatat ttacagtcat
    11161 ggcatgatcc aattctatag actcaaccgg ctctccattc acatacaaat caagtttttt
    11221 gtcgaaacta ctatagacca acgaaatcat ataccaatga tgtgttttaa aaggatagcc
    11281 gaacgcctta ttgatattac cgccaataaa tgcgtatagt ttaccatttt cacgaaattc
    11341 ataatgtagc ttacctcggc tccagttttt atttgtccaa atcatattca tctcgctaaa
    11401 tgatgttgga taaataaggg tttcaagggt aaattcaggc aattcaataa aaccagggtc
    11461 attaagttga agatgcgcag tcatagcatt aaaatctatg gccttttcaa attccgtctt
    11521 tgaattaacc aacacttcca catcggggcg gggagggtta atcattgtaa gcgatgttga
    11581 aagggcaatc tctcctaacc cgttaatatt atattccatt ttaagttgtg cattaaccga
    11641 aggtagaatg cagttcgtcg aaaatatcgc ttctgattta ttcaatattt tgaagcgtga
    11701 tgtcatatct tgcgaaaact ctgaagcctc tttccatggt gcgcatcttt tatccatgcc
    11761 atttccaaag tcattatctg taggtgctcg ccagaagttg gctttcagtg gttcattaat
    11821 tactgctttt ccgtcaataa gccaattgga gagttctccg ctctctctgt caaaagaaat
    11881 ctgaaagttt cttccctcaa acactaaaag attagatgac tcattaactt taagttcggg
    11941 tatatttctt atcggacggt cgagtgaggg ttcgtaattt ggtatctcaa tttgctcggc
    12001 agcaacttca aaattggctg gaatgatttc gtcagaagct ttagttaaaa aactaaaatg
    12061 gataaaatat tctttttcgg gtttaaaact caaagaagag aagtccagat ttagagtaaa
    12121 actctcatgt gcacctactg gcaggcgatc aaccactccg tttaagattt gttttccttc
    12181 ggatttaatt tcccaaatga ggtagtagtg tttaaggctt gtaaaatcgt atttgttgat
    12241 gatatgaaat tttccggcta acggattaat tgtttttaca tcaatatttt gataaacttt
    12301 tttcacctca aaaaggcctg ggtgcggtgt acggtcgggt gaaaccaatc cgttaataca
    12361 gaaattgccg tcgcttggtg ttccctcagg tccaaaatcg ccgccgtatg cataaaactg
    12421 tgagccatct gatgcttttg tcaataaccc ctgatctacc caatcccaca caaatccgcc
    12481 ttgtagttga ttatatctat caataagttc ccaatactct ttcaggttgc cactgctatt
    12541 tcccattgca tgtgcatatt cacacattat caacggcctg tcctgatgtt ttcgaacata
    12601 atcttcgata tgttcaacac ttgcatacat tggacaataa atatcagtat tagggcctaa
    12661 taatgcccgt tcgtactgta cagggcgtga gggatctcta aactttaccc attgataaca
    12721 agcagaaaaa ttaactccat cacctgcttc attcccaagt gaccaaatga taattgaggg
    12781 atggtttttg tctctttcaa ccattttttt cacccgatct aaatgtgctg ttccccagag
    12841 aggatcttta gccaaacttc gatcaccata acccatccca tgcgattcta tatttgcctc
    12901 gtccacaaca taaagcccat accgatcgca taggtcatac catcgtggat cgttaggata
    12961 atgagcggta cgaacagcat taatattatt tttcttcatc aaaaagatat cctctttcat
    13021 catctcttcc gaaataacat gcccggttag tggatcatgt tcgtggcggt ttactccttt
    13081 cagggtaacc gctgtgccgt ttatgagtaa ttgcccgttc ttaatttccg aagttcgaaa
    13141 accaacattt gaagagaatg cttgtaatat gttacccttt ttatcagaaa gagttatgac
    13201 taatttataa agttcagggt tttcggcgct ccattttctg ggattttgaa taaaaacgtt
    13261 aaatttaatt tcgccgctct cttttgcagc aagtttaatc gttttacttt cctgataaat
    13321 aacagcatcg gctgagctac caaaaagatg aatagtggaa tttagtttcg gtgttttttt
    13381 cttattgtca ttatctattt tcaacaacac acttaactgg ccatctcgat attcattaac
    13441 caatcctgca tttacctcaa aatcgctgat attaaccaaa ggcacgctgt aaagaaaaac
    13501 atccctttgt atcccgctaa tcctccaaaa gtcctgacat tctaaatagg atccgtcgct
    13561 ccaacgaata acctgcgctg caagtttatt tttaccttta cgtatatatg gagttatgtc
    13621 aaattcagcc ggtagcttgc catcctgact atatcccacc tcttcgccgt tgatccaaac
    13681 atataaagcc gatttcgcac caccaaaatg aaggattatc ttacctccca accagttttc
    13741 gggaacttcg aaaaaagtga tgtacgaccc ggaaggattc tcaatatgag gtattgtagg
    13801 tggttgtggg tcgtaagtcc attcataagg ttgatttaca taaatcggaa taccgtatcc
    13861 ttgaatttcc cagttagagg gaacagaaat ttcgtcccaa tcactgaagt catattccgt
    13921 aagataaaat ccatcaggtc gttgatcgag cctgtcagaa tacttaaact tccattttcc
    13981 atttaacgac atgaaataag gcgaactatc ccaaatatta tcaattgcct gatttaccga
    14041 agaataagga attgcaaaag acctagcagg aagcttattc ctgctaacta cctccgggtt
    14101 ttcaatatca ttaaatatat tgttatgttg agtaaaggcc ggaacagcga aaaaaaagag
    14161 aaaagtaaaa aaaagagaag tagtaaatat tttcatagcc taagtgaatt aattttgttt
    14221 tttattacta aaaactgtgc acaaatttaa tcggaaatcc cttcacaaaa aaatttgttc
    14281 aatattattt ggattggata attaatcggt atcctacatt gtggatattc tcaattttta
    14341 ctttgggatc ttctctaaga tatttccgta atcttgaaac aaaaacatcg aggctgcggc
    14401 tcgcaaaata atggtcatca ccccaaactt tcatcagaag atcatctctt tttacaatcg
    14461 tatccctatt ttcacaaaac atacataaaa gttcactctc acgctgggta agctctttta
    14521 ttttattatt aaaagtaaga cgctgatttt taacactaaa tgtatattct ccaatattat
    14581 gtacctctat agactccaat cggggctgtc cgcttctttt caggaatact tctattttaa
    14641 gaattagctc ttcaatacta aagggcttgg ttatataatc atcgccacct aaccttaatc
    14701 cgtgtatctt atcttcttta gttgatttgg cagtaagaaa aattatcggg atatcaacat
    14761 ctttctccct tatgctggca gccacttcaa aaccgtccat ctttggtaac attacatcga
    14821 ggatacagag atcgtaatga ttggcattat aaagtttcat tgcctgctcg ccatcttcgc
    14881 aaaaatctat tgcataacca tgcgattcaa gattgtcgcg ggttacgaat gacaatgttt
    14941 gatcatcctc aacatataat attttagctt tagtcataat taaatcattt cgattataaa
    15001 atcagacccc ttcccgactt cactctccaa ataaagcttc catccgtgcg cttcacacac
    15061 tttaagcaca taataaagcc ccagaccaaa accttttaca ttgtgtacat tacctgtcgg
    15121 gatacggaaa aacttttgaa aaattttctt ttgatattca gtgtctattc ccggtccgtt
    15181 atctgaaaca ataagtcgaa ttttatctct gttcatactt gttgtaacag taacttgtgg
    15241 aacaacaccc gaatacttta ctgcattatc caacaggtta taaataatat ttgtaagatg
    15301 aagcttatca gccaaaacct cttttatttc tgggctcaat tggacagtga aagtaatgga
    15361 tctcccattt gcacactgtc tttggtggtt ctctactact tcagttatca aattattaag
    15421 ttgtatcgct tcctttttca attcatagcc gcctttttcg atatgcgcaa tttgcaaaac
    15481 cttttccaca agattattta accgttgatt ttcttgttta ataatagcgc tgtaactttt
    15541 caaacgttgc ggctgttcaa taatattggg cgaagcaagt acatcagcag aaatatttat
    15601 gctcgagatg ggtgttttaa attcatgggt catattatta ataaaatcct tctgaagttc
    15661 cgaatatctt ttttgctgta aaatgataaa tatagaataa acgaaaaaaa tgaccgatac
    15721 gaataatatt gccgtaaaga taaaccagag ggtcatatct gcaccaagat aattccgcat
    15781 agaggggaaa ttaattccaa agtaatagat gtaatcactg tacttgggca ggtcaactga
    15841 catgttgatc ggtttgttct cgcctttggc agaaacatat tttccataca ccatcttatc
    15901 gtcatggcaa tcgtaaatgg catactcata atcagttata atattatgaa ttgaaaattc
    15961 ggttttcaga taatattcaa ggatatttgc atcaattaca ctgttcagat taaccacaaa
    16021 atagcttgcc gacaactggt ttaccgggtt ggtggcaggt aagacggatt gattgtaccg
    16081 gctcatcttt tcggctacct caaacagcgc aatatgaacc gtttggctga actgtttttc
    16141 tttgatattc catgtattgc gcaaccagta cacctgtact actattatac ttgtaattgc
    16201 aaatgctcct aaaataacaa tgaatcgaat tttattgcgt gtcatgttac aagtcggtaa
    16261 ttatttggaa ttgctaaagt aaacgaaatg aaattgatta atcatatatt tacattagtg
    16321 tccactaaaa attaaacgat aattgttgat tataactgtc ttcattt
//
LOCUS       DAOOAI010000047        16222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_4678_length_16222_cov_5.251191, whole genome shotgun sequence.
ACCESSION   DAOOAI010000047 DAOOAI010000000
VERSION     DAOOAI010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 16222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16222
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4678_length_16222_cov_5.251191"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(400..2649)
                     /locus_tag="PK345_04830"
     CDS             complement(400..2649)
                     /locus_tag="PK345_04830"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0015891 - siderophore transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPX34376.1"
                     /translation="MKKILFFISIFILIQLNASAQKASGRVFELDENNKKKPLTGVNV
                     YWAHSLQGTTTDSKGYFNISKQESDYARYDHEHEDDHSEHLLVFSFVGYLNDTIHVHQ
                     DQRNIEIILATITELEGVEVSARQSGSFISRKDPIVTQQINRAELHKAACCNLSESFE
                     TNASVDVNYSDAVTGAKQIQLLGLSGLYSQLMTENIPNFYGLANSYGLMWVPGTWMES
                     IQISKGTAAVINGYESITGQINIEYKKPDNSEKLFINLFADNEGRIETNINTSTKLDK
                     NWSTMIYGHVSHNNFRKDFNNDGFMDNPLYTQYNLFNRWKYSDEHYEAQFGIHYIDEN
                     RISGQMDFDIDEKRSADKPYGINIHNKRLQAFAKNGIIFHRPATSMALINSFTLQDQN
                     SFFGLRDYTARQNSSYNNLIFQSYLGNTKHSYTTGLTYIYNNYDEMITDSSFSMKESV
                     PGVYFQYTYSMPDKLTVITGIRADFHNEFGAFFTPRMHVRYTLNPNTIIRVAAGKGYR
                     TPNVIAENISFLASSRDIKPLEKLQQEEAWNYGMHLTQYVNLLGKQMTISADFYRTNF
                     VNQVIFDIDSDVSQIRLYNLDGKSYSNSAQLEINYELLKRLDMVAAFRLTDVKTTYEG
                     DLLRKPLISKYKGLVTLSYATYRNLWQFDFTSQFNGGGRLPKTIGNPPEYQLEETFPA
                     YTILNAQITRYLKKWSIYLGGENLLGYRQRHPIVAANDPFGNYFDASMVWGPIMGAKI
                     HIGLRFAIE"
     gene            complement(2768..3292)
                     /locus_tag="PK345_04835"
     CDS             complement(2768..3292)
                     /locus_tag="PK345_04835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34377.1"
                     /translation="MKVFRQILALIIALVFFITTSGLNFYTHICGYCQSFDVSLTDFT
                     ECCDHNESVACKISPIVSKSYCNNSGKPLCKSDYQFYKNDGCCLYKHSFFKIADSFNY
                     SEQISLKQNILSLSIIDFSFQIPFVLPETHRVINPKYSHPPPLIAGKDRIIYIHALKL
                     APSFDFHTSEYFAA"
     gene            3387..4163
                     /locus_tag="PK345_04840"
     CDS             3387..4163
                     /locus_tag="PK345_04840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HPX34378.1"
                     /translation="MNFEILQLSVEQNVALVTISRPSALNALNTQFFNEMDAMIEKIN
                     SMSEIKVMVITGVGKAFVAGADIAEMVNKNQEEGTAFSKLGQNIFRTLEEMEIPVIAA
                     INGFALGGGLELAMGCDFRIASNLAKFGQPEVNLGLIPGYAATQRLPRLVGMADALYL
                     LMTAEMIGADDALRIGLVQKVVEPELLIEETMKLAKLICSKGPKAVKLIKKVVREGME
                     GNFKDGCELEAINFGSLFGKGNEGEKGMRAFLDKVKPKWE"
     gene            4347..5684
                     /locus_tag="PK345_04845"
     CDS             4347..5684
                     /locus_tag="PK345_04845"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HPX34379.1"
                     /translation="MTYDERMQKVSVLGAAGKMGSGILLLTAVEMCNLSLKPENRGKS
                     FVLYAIDISEEGLNGLMKYLKVQIRRIAEKKMVWLREVYAHRADLIENGQIIEEYIFD
                     VMNLVRPITAIEAAFNSNLIFEAIAENQDFKVKIFKKIDQNNQGKPWIFTNTSSIPIS
                     VLNKEAGLDGRILGFHFYNPPAVQHLVELITIKENTRQMKEFAIAYAKSLKKVVVPSN
                     DFAGFIGNGHFMRDALHCIAEAENLMKEFSFAESIYIINKISQDYLVRPMGIFQLIDY
                     VGIDVVRFILAVMNPYNPSENLHSPLLDSLFDLNVKGGQNSDGSQKDGLLKYEKGKPV
                     AVFDLQKRDYISISEFSAKCDEYLGALPEKWQPWKSVNFSKNKEELLKAYFAAIKGMD
                     TVGAQLAKVCHNRSAQIGKQLVNSGVANSDEDVNTVLLTGFYHAYGPINNYLQ"
     gene            5696..6940
                     /locus_tag="PK345_04850"
     CDS             5696..6940
                     /locus_tag="PK345_04850"
                     /inference="COORDINATES: protein motif:HMM:NF014820.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34380.1"
                     /translation="MKKMQKKIYMAAGYNTISMGTGRKEFNPKKERPGLEYYITEAGR
                     GTLAQINGAVNVDESVIGNFMASRFNNQGHLAGFIPMIDEGLKFKPSIRVEAACASGG
                     MALITGIKNVLAGADTVLTIGVEVQNTMKAIYGADVLAGAGWYKKRKEGHAYFFPDQF
                     SKRAGAYFDKYGKDYTRKGLSRWYANAIENARLCPTAQEYHNTNPDPESTCFKMEPNG
                     KSFVDYLNVLDCSKVSDAASAIAILSETGLIKCGIDRKDAVEVVGFATSERNITLEPS
                     DLTVMETIKDAAHKALANAGVSIHDLATIETHDCFTIAGILSVEALGFAKPGEGADFV
                     FKGNTARNGLIPINTTGGLIGWGHPTGGTGVHQAVTIWEQLTGRTGEAQIEISEKKPY
                     GLSINMGGDDVSVVTIVYKKEL"
     gene            7063..8409
                     /locus_tag="PK345_04855"
     CDS             7063..8409
                     /locus_tag="PK345_04855"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014226422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HPX34381.1"
                     /translation="MVTVNISNIFDFVDKQRVLSFQKIISKNHQALLEKIGAGNNFLG
                     WLTLPSDIDDALLRDIDSTAVRIRELADVFVVVGIGGSYLGARAIIEALKHNFNQLLQ
                     TKGAKNPIILYAGQNLSEDYLADLFDVLDRYDYAINVISKSGTTTEPAVAFRILKNHI
                     EKKYGNRARERIIVTTDSSKGALRKMANDLGYKSYIVPDDIGGRYSVLTPVGLLPIAV
                     GGFDIKALVNGAKKMQQQLAETTDIERNPAALYAASRNALYMQGKFIEIMVNYQPNLS
                     FFAEWWKQLYGESEGKEGKGIFPASVINTTDLHSMGQYIQDGERKIFETVLSVGKSKR
                     KLLIPAEKEDLDQLNYIAGKNIHYVNQMAERGTTLAHLDGGVPCIEISIDKVDEYHLG
                     GLIYFFEFACALSGYTLGVNPFDQPGVEAYKRNMFALLGKPGFEKESMEIQKRLKK"
     gene            complement(8419..9147)
                     /gene="rnc"
                     /locus_tag="PK345_04860"
     CDS             complement(8419..9147)
                     /gene="rnc"
                     /locus_tag="PK345_04860"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR02191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HPX34382.1"
                     /translation="MYNRIKAQFSSDKNLIISLRNIFGFWPENIALYKLALIHRSAAT
                     EQSGHKISNERLEYLGDAVLSSIVADYLFKKFPYREEGFLTEMRSKIVSREQLNKLAL
                     KLGIDHFIISTQDSKTYFRSMPGDAFEALIGAIYLDKGYIFTRKMVINRIINVHFDID
                     DLEKLENNFKSKLIEWAQKEHKEIKFNVVAEVGSGNRKQYFVEITIDEKLYATGCDFS
                     IKGAEKNAAEKTFIQLQLDKTSEN"
     gene            complement(9162..10418)
                     /gene="fabF"
                     /locus_tag="PK345_04865"
     CDS             complement(9162..10418)
                     /gene="fabF"
                     /locus_tag="PK345_04865"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HPX34383.1"
                     /translation="MNLKRVVVTGLGAITPLGNTVEELWDSLINGVSGAAGITRFDAS
                     KFKTQIACEIKGYDPANYFDRKESRKIDPYTQYAMIAADQAIADANFNMDTLDKDRVG
                     VIIATGIGGFTSFLQEVIEFAKGDGTPRFNPFFIPKIIGDIAAGHISIKYGFRGPNYA
                     TTSACASSGHAIIDAFNQIKLGKSSVFVVGGAEAGINPAGIGGFNAMHAISTRNDDPK
                     TASRPFDKDRDGFVLGEGGGVIILEEYEHAIKRGAKIYVEIAGEGSTADAHHITSPHP
                     DGLGAYMAMKLAVEEAGIKPEDVDHINTHGTSTPVGDIAEQKAVVNLFGQHAYKINLN
                     ATKSMHGHLLGSAAAVELIATILAIKNSIIPPTINHFTDDPDIDNNLNFTFGNAVKRE
                     VNFALSNSFGFGGHNTCIALKKFQDK"
     gene            complement(10433..10669)
                     /locus_tag="PK345_04870"
     CDS             complement(10433..10669)
                     /locus_tag="PK345_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HPX34384.1"
                     /translation="MSDIATRVKAIIVDKLGVDENEVTLQANFTNDLGADSLDTVELI
                     MEFEKEFNIAIPDDQAEKIGTVGDAIAYIENNAK"
     gene            10915..11505
                     /gene="purN"
                     /locus_tag="PK345_04875"
     CDS             10915..11505
                     /gene="purN"
                     /locus_tag="PK345_04875"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HPX34385.1"
                     /translation="MNRIAIFASGNGSNAQKIAEYFSNINLAKVTMILSNNPDAFVLH
                     RARQFGVATKVFDRKQFYHSEEVLRCLKENKIDLIVLAGFLWLIPDYLIRSYPKKIIN
                     IHPALLPKYGGKGMYGDAVHKAVISAGESESGITIHYVNKDYDKGEVIFQARCKIQEN
                     DTAEVLAERIHNLEYQHYPKVIEAILTDAPYPTKTG"
     gene            11779..12204
                     /locus_tag="PK345_04880"
     CDS             11779..12204
                     /locus_tag="PK345_04880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPX34386.1"
                     /translation="MVLTRKYELLKPVNWRHRIILIVDDVIPNFKILEKALLPTQAKI
                     EHAKIGFEGIRKCLENQKIDVVLIEINLSELSELELVNQYRVFRPDLVIIGHSAFVTT
                     VTRQKCLEAGMDDFFNKPIDSKELIVIIGMALKKRDKKL"
     gene            12429..13964
                     /locus_tag="PK345_04885"
     CDS             12429..13964
                     /locus_tag="PK345_04885"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HPX34387.1"
                     /translation="MMKRLTIISLVLFFFFNILSAQAQNQVDPDGFNRFYYPNGQVSS
                     EGYLRNGQPDGYWKTYFENGSLKSEGNRIDFLLDSTWTFYDENGNKTLEVNYKAGMKE
                     GKRFTYRENETIEENFSNDIKQGQTIYYYLDNRIFRIINFENGLEEGMAKEYADDGRV
                     ILITTYRKGFLISRERINRLDSEGRKQGHWKFFHKNGLVKLEGFYLNDLKNGFFKEYD
                     SAGKLVEATKWLKGEIQEQAEELTKLEVSKEYYPDGKIAVFQTFKNGIPHGVKRDYSK
                     EGTIVSGSLYKEGKLIASGITREDGVRNGAWKDYYSDGKLKAEGNYKDGKMIGKWNFY
                     HVNGKIEQIGEFDKQGRPVGKWLWYYPSGNLLREEHYIGGKADGMMSEYSETGDLIAE
                     GDYIDGEEEGQWKYNIGNFKEEGGYSYGLRHGLWKHYYNDGTLMFQGEFINGKPHGKH
                     TVFWDNEKIKDETTYEMGIKQGNSYSYNYSGELILVITYQDGAEVSYDGIKVDPLILG
                     EDE"
     gene            complement(13980..15068)
                     /gene="dprA"
                     /locus_tag="PK345_04890"
     CDS             complement(13980..15068)
                     /gene="dprA"
                     /locus_tag="PK345_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008156367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HPX34388.1"
                     /translation="MLLQQIALTLLPGVGNVTGKKLIAYCGGVEAVFKQKREALEKIP
                     GIGEKLINSILNHFVFRQAEKEIAFIEKNNITPLFYLDKNYPELLKNCDDGPILLYYK
                     GTASLNVSRIISIVGTRKATDYGKMICEKLVKDLAEYNVLVISGLAYGIDTCAHHSAL
                     DNHLNTIAVLGHGLDRIYPHLNRNLATKIIDHGGLLSEFMSGTLPDRENFPQRNRIIA
                     GMADATVVIEAAKSGGALITADMANSYNREVFTIPGRIGDTWSEGCNLLIRDNKAALI
                     QSATDIAKMMGWEKKKQNVKQHLLFVNLTPDEKKIISLMRGNGEMGIDEITLQSRLNM
                     SKVAAALLNLEFNGIVKALPGKIYKFIG"
     gene            15191..15979
                     /gene="lgt"
                     /locus_tag="PK345_04895"
     CDS             15191..15979
                     /gene="lgt"
                     /locus_tag="PK345_04895"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HPX34389.1"
                     /translation="MLSYITWSVNPEIFPNLLPVRWYGLLFAAAFFFGYLVLIKIFKH
                     EKLPVALLDQLALYVFIATIIGARLGHVLFYEPDYFLSNPLLILKIWQGGLASHGAAI
                     GILIAIWLFSRKNKIHYLWVLDRLVIVVALGGFFIRMGNLMNSEIYGRATLLPWGFIF
                     TSQDNIPRHPTQIYEGLSYLAIFGYLLWHYWRKNGKPRQGYLFGMFLILLFTVRFLIE
                     FVKEPQVDFETDMLLNMGQLLSIPFIIAGVIVVFRKERKPTKNN"
     gene            16027..16100
                     /locus_tag="PK345_04900"
     tRNA            16027..16100
                     /locus_tag="PK345_04900"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:16061..16063,aa:Ala,seq:ggc)
BASE COUNT         5089 a         2655 c         3204 g         5274 t
ORIGIN      
        1 caatgttgat cagcatcatg cttgcagcat caggtaaatt ttgataagct ttttcaactg
       61 ctttaagatc attagaattg taaccggatt ttggtaattg ctgttttaat tttattgacg
      121 ttagtttttt cagacaataa tcatcagtaa taagattttc tgagatattg acctgaatat
      181 tggatattac tttttcaaaa aaaaagttct ctttttaatt gtctttttaa atgtgccgca
      241 cactacctaa gagtgaatct gaacggtttc agttttcttt ttctcttgag atttgactgt
      301 atttgggtga ataaatacaa aagcaagagc taatattgtt atttaaacaa gacctgtttt
      361 tttatgattg ataagttttt aaaaattcga attttgattt tactcaatag caaaacgtaa
      421 tccaatatgt atttttgcac ccatgattgg tccccatacc attgatgcgt caaaatagtt
      481 accaaaagga tcattggcgg caactattgg gtgccgttga cgataaccta aaaggttttc
      541 acctccaaga tatatgctcc atttttttaa atatctggtg atttgtgcat tgagtatcgt
      601 ataggcaggg aatgtttcct cgagttggta ttcgggcggg tttcctatgg tttttggtag
      661 tcttccgcca ccattaaact ggctggtaaa atcaaattgc caaagatttc tgtatgttgc
      721 ataggataaa gtaaccagtc ctttatattt gcttatcagg ggtttacgta aaagatcgcc
      781 ttcataggta gtttttacgt ctgttagcct aaaagcagca accatatcaa gtctttttaa
      841 taattcataa tttatttcta actgagcgct atttgaatat gatttgccat cgaggttata
      901 aagcctgatc tggcttacat cactatcaat atcaaaaatt acttgattta caaaattagt
      961 tcgataaaaa tcagcgctta tagtcatctg ttttcctaaa agattaacat attgtgtcaa
     1021 gtgcatcccg tagttccagg cctcttcttg ttgtaatttt tcaagaggtt taatatctct
     1081 tgaactggcg agaaacgaaa tgttttcagc aattacatta ggtgtacgat agccttttcc
     1141 ggcagctact ctgataatgg tattgggatt caaggtatat cttacatgca ttcgaggcgt
     1201 aaaaaaagcg ccaaactcat tatgaaaatc tgcacgtatt cctgtaatca ctgtcagttt
     1261 atcgggcatg ctgtaagtat attgaaaata tacacccgga acactttcct tcattgagaa
     1321 agagctgtca gttatcattt catcgtagtt gttgtagatg taggtcaggc cggttgtata
     1381 actgtgtttt gtattaccta aatatgattg aaaaatcaga ttgttgtacg agctgttctg
     1441 tcttgctgtg taatctctaa ggccaaaaaa agaattttga tcctgcaagg tgaatgaatt
     1501 aatcaatgcc atactagtgg ctggccgatg aaagataatc ccattttttg caaatgcttg
     1561 taaacgttta ttatggatat ttataccgta tggtttgtcg gctgatcgct tttcatcaat
     1621 atcgaaatcc atttgtccgc taatcctgtt ttcatcaata taatgtatgc caaattgagc
     1681 ttcgtaatgt tcgtcgctat atttccatcg attaaagaga ttatactggg tatagagtgg
     1741 attatccata aatccatcgt tgttaaagtc ttttcggaaa ttattatgac ttacatgtcc
     1801 gtaaatcatt gtactccaat ttttatcaag ctttgtagaa gtgttgatat tggtttcaat
     1861 acgtccttca ttatcagcaa agaggtttat aaaaagtttt tcactgttat ctggtttttt
     1921 atactcaata ttgatttggc cggttataga ttcatatcca ttaattacgg cagcggtacc
     1981 tttagatatc tgaatacttt ccatccatgt ccccggcacc cacatgaggc catagctatt
     2041 ggcaagacca taaaaatttg gaatattctc cgtcattaac tggctgtaaa ggccgcttaa
     2101 tcctaatagt tgaatttgct ttgcaccggt aacggcatcg ctatagttaa catctaccga
     2161 agcattagtc tcaaaacttt cggatagatt acaacaagct gccttgtgca actcggcacg
     2221 attgatctgt tgagttacaa taggatcttt ccttgaaatg aatgatccag attgtcttgc
     2281 gctcacttca acgccttcca gttcggtaat agttgcaagt ataatttcta tatttctttg
     2341 atcttgatgg acatggatcg tgtcattaag ataaccaaca aaactaaaaa ctaaaaggtg
     2401 ctcactatga tcgtcttcat gctcgtgatc gtaacgcgca taatcacttt cctgtttact
     2461 tatattgaaa tatccttttg agtctgttgt tgtaccctga agactatgtg cccagtaaac
     2521 attgactccc gttaaaggct tttttttgtt gttctcatcc agttcaaaca ctcttcctga
     2581 agctttttgg gcagaagcat tcaattgaat cagaataaaa atgctgataa aaaacaatat
     2641 ttttttcatt tgaattttca tgttattaat aaatcaaact ttgaagaatt agtttttatc
     2701 cacaaaaacc taaagagtaa cgaagttttt gtggtaaaaa aaatggaaat actatgcatg
     2761 ccgaaaatca ggctgcaaag tattcggagg tatgaaaatc aaatgaagga gccagtttta
     2821 atgcgtgtat gtagataatc cgatcctttc cggcaattag tggcggcgga tgcgagtatt
     2881 taggatttat tacccgatgg gtttcaggta aaacaaatgg aatctgaaaa ctaaaatcaa
     2941 ttatcgaaag tgataagata ttttgtttaa gtgaaatctg ttccgaatag ttgaagctat
     3001 cggcaatttt gaaaaaacta tgtttgtaca ggcaacaacc atcattttta taaaattgat
     3061 aatcgctctt gcataaaggc ttcccactat tgttacaata tgattttgaa acaatgggtg
     3121 aaattttgca agcaacactt tcattatggt cacagcattc agtaaagtca gtaagtgaga
     3181 catcaaaact ctggcaatac ccacaaatat gagtgtaaaa atttaatccg cttgtggtaa
     3241 tgaaaaacac aagtgcgata attaaagcca gtatttgacg aaaaactttc ataaatagaa
     3301 gtaataatga tgtaaacgaa aaaatattta atttggttta atgagtttta ttaagtttga
     3361 caaaaattat cttaaacaat acatttatga attttgaaat tttacaactt tctgtagagc
     3421 aaaatgttgc gttagttacc attagtcgtc catctgcctt aaatgctcta aatactcagt
     3481 tttttaatga gatggatgct atgattgaaa aaataaatag tatgtccgag atcaaagtaa
     3541 tggtaataac cggtgtgggc aaagcttttg ttgctggcgc tgacattgct gaaatggtta
     3601 ataaaaatca agaagagggc actgcgtttt cgaagcttgg acaaaatatt tttcgtactt
     3661 tagaagaaat ggaaatacca gtcattgctg ctattaatgg ttttgcactt ggtggtggcc
     3721 ttgaacttgc tatggggtgt gactttagaa ttgcaagtaa tcttgcaaaa ttcgggcagc
     3781 cagaagtaaa ccttgggtta ataccaggct acgctgctac gcaaaggtta ccaaggcttg
     3841 taggtatggc tgatgcactt tatcttttga tgacggctga gatgattgga gctgacgatg
     3901 ctttgcgtat tggcttggtc caaaaagttg ttgagcctga attgcttatt gaggagacta
     3961 tgaaacttgc gaagcttatt tgctcaaagg gcccaaaggc tgtaaagctt attaaaaagg
     4021 tggttagaga gggtatggag ggtaacttta aagatggttg tgaacttgag gcaataaact
     4081 ttggatcgct atttggcaaa ggaaatgaag gagaaaaagg tatgagagct ttcctggata
     4141 aagtgaagcc aaaatgggaa taattttttt aattgtattt cgaatattat tatgtacaac
     4201 tgtgaattca gttaacttag gattaaaaag cagccatttt gtaaatgatt tgaatacaat
     4261 tatcgtaaaa taatagggaa aaaatcattt ttatatttac atcgtaaata atcaagcagt
     4321 taaatttaaa cttaaagaat aaaaaaatga catacgacga aagaatgcaa aaggttagtg
     4381 tgctgggggc tgctggaaag atgggtagcg gaatcttgct gctcactgca gtggaaatgt
     4441 gcaacctcag cctaaagccc gaaaatagag ggaaatcatt tgtattatat gcaattgaca
     4501 tctctgaaga gggattgaac gggcttatga aatacttgaa agttcagatc agaagaattg
     4561 ccgaaaagaa aatggtatgg ttgcgcgagg tttatgctca tagggctgat cttatagaaa
     4621 acggccaaat tattgaagaa tacatttttg atgtaatgaa tttagtaagg cctattaccg
     4681 ctatagaggc agcttttaat tcaaatctta tttttgaagc tattgctgaa aatcaggact
     4741 ttaaagtaaa aatttttaaa aagattgatc aaaacaatca ggggaaacca tggattttta
     4801 ctaatacatc ttcaattccg atcagtgttt taaataaaga agcagggctt gatggtcgta
     4861 ttcttgggtt tcacttttac aatcctcccg cagtacaaca ccttgttgaa ctgattacga
     4921 taaaggaaaa cacacggcaa atgaaggaat ttgcaatcgc ttatgcgaaa agccttaaaa
     4981 aggtggtggt gccttccaac gattttgcag gatttattgg caatggtcat tttatgcgcg
     5041 atgcattgca ttgtattgct gaagcagaaa atcttatgaa agaattttca tttgccgaat
     5101 caatttacat aattaataaa atttctcaag actatctggt tcgccccatg ggaattttcc
     5161 agttaattga ttatgttgga attgatgtgg ttcgtttcat tttggcagta atgaatcctt
     5221 acaatccgtc tgaaaattta catagtcctc tgctcgatag tctttttgac ttaaatgtta
     5281 aaggaggtca gaattctgac ggctcgcaaa aagatggtct tcttaaatat gaaaaaggta
     5341 agccagtagc cgtttttgat ctacaaaaaa gagattacat atcaatttct gagttttcag
     5401 caaaatgtga tgaatatctt ggcgctttgc ccgaaaagtg gcagccatgg aaatcggtaa
     5461 acttctctaa aaataaagaa gagcttctca aggcatattt tgccgctatt aaaggaatgg
     5521 atactgttgg agcacaattg gctaaagtct gtcataatcg ctcggcacaa attggtaagc
     5581 agctggttaa tagtggagtt gctaatagcg atgaggatgt caatacagtt ttgctaactg
     5641 gcttttacca tgcttatggt ccgataaaca attatttaca ataaaaaaga caatcatgaa
     5701 aaaaatgcag aaaaaaatat acatggctgc tggttataat actatttcta tgggtaccgg
     5761 ccgtaaggag ttcaatccca aaaaggaaag gcctggtttg gagtattata ttactgaagc
     5821 cggacgcggg acattagctc agattaacgg tgcggtaaat gttgacgaga gtgtgattgg
     5881 caatttcatg gcttcgcgtt ttaataatca aggacatctt gcagggttta ttccgatgat
     5941 tgatgaaggc ttaaagttca aaccaagcat cagggtcgaa gctgcttgtg catcgggtgg
     6001 catggcgctt attaccggaa taaaaaatgt gcttgccggc gccgatactg ttttgacaat
     6061 aggtgttgaa gtacaaaaca ccatgaaagc tatttatggc gctgatgttc tggctggcgc
     6121 cggatggtat aaaaaacgta aagagggaca cgcctatttt tttcctgacc aattcagtaa
     6181 acgtgctggt gcttatttcg ataaatatgg aaaagattat actcgcaaag ggctttcgcg
     6241 ctggtatgct aatgcaatag aaaatgcaag actttgtcct accgcgcaag agtatcataa
     6301 tactaatcct gatcctgaaa gtacctgttt caaaatggaa ccaaacggta aatcttttgt
     6361 agattatttg aatgtccttg attgttcaaa ggtgtccgat gcagcctcag ctattgctat
     6421 tctttcggaa accggattaa taaaatgtgg tattgataga aaagatgctg tagaagttgt
     6481 tggctttgct acttccgaac gtaatataac tttggaaccg agtgatctaa ctgtgatgga
     6541 gactattaag gatgcagcgc acaaagcact tgctaacgct ggggtctcta ttcacgattt
     6601 ggcaactatc gaaacgcatg actgttttac tattgccggc atactttcag tggaggctct
     6661 tggttttgct aaacctggcg aaggcgctga ttttgttttt aaaggtaata cagctcgtaa
     6721 cgggctaata ccgataaata caaccggtgg gttgatagga tggggacatc ctactggagg
     6781 aaccggtgtt catcaggcag taacaatttg ggaacaactt acaggaagaa ccggtgaagc
     6841 tcaaatagaa atatcagaaa aaaaacctta tggactttct attaatatgg gtggtgacga
     6901 tgtgagtgtg gttacaattg tttataaaaa agagttgtaa catccgattt taatagtcat
     6961 tgatcgttat aaaaaagtag aaataacttt tcttaatatt tcatttgaga gacttgttta
     7021 aaagaggtaa ttttggtttt taaacaataa agcttttaaa aaatggtaac tgttaatatt
     7081 tctaatattt tcgattttgt tgataaacaa agagtattat cgtttcaaaa gataatttct
     7141 aaaaatcatc aagcgctttt ggaaaagatt ggagcaggaa ataatttttt aggatggtta
     7201 acgcttccaa gcgatattga tgatgcgtta ttacgtgata ttgattctac tgcggtaaga
     7261 ataagagaac tagccgatgt gtttgttgtt gttggtattg gaggttcata tcttggcgcc
     7321 agagccataa tagaagcatt aaaacacaat ttcaatcagt tgctccaaac aaaaggtgca
     7381 aaaaatccta ttattcttta cgcaggtcaa aatctgtcgg aagattatct ggctgatcta
     7441 tttgatgtgc ttgataggta tgattatgcc atcaatgtta tttctaaatc gggtactact
     7501 accgaaccgg ctgttgcttt tcgcatttta aaaaatcata ttgaaaaaaa atatggaaat
     7561 agagcgagag agagaataat agtaactacc gatagttcaa agggtgcttt gcggaaaatg
     7621 gcgaatgacc ttggatataa aagttacatt gttcctgatg atataggagg gcggtattct
     7681 gttttgacac cggttggctt attacccatt gctgttggcg gatttgatat aaaagcattg
     7741 gtaaacgggg cgaaaaaaat gcaacaacaa ttggcagaaa cgacagatat tgaacgcaat
     7801 ccagctgcac tctatgccgc aagccgaaat gccctctata tgcaaggtaa gtttattgag
     7861 ataatggtaa actatcaacc gaacttatca ttttttgctg aatggtggaa acagttgtac
     7921 ggggagagtg aaggaaaaga agggaaagga atttttcctg catctgtaat taatactacc
     7981 gacctgcatt cgatggggca atatattcag gatggggagc gcaaaatttt tgaaactgtt
     8041 ttatctgttg gtaaatcaaa acgtaaactg ttaatccctg ctgaaaaaga agacttggat
     8101 caattgaatt acatagctgg gaaaaatatt cattatgtta atcagatggc cgaacgagga
     8161 accacccttg cccatcttga tggaggagtt ccttgcatag agatctccat tgacaaggta
     8221 gatgaatacc atcttggcgg tttgatttac ttttttgagt tcgcctgcgc attaagtggt
     8281 tataccttag gcgtaaatcc ttttgatcag cctggagtag aggcctacaa acgcaatatg
     8341 tttgctctat tagggaagcc cggttttgaa aaagagagta tggaaataca gaagaggtta
     8401 aaaaagtaaa aaaagcagtt agttttcgga agtcttatca agttgtaatt gaataaatgt
     8461 tttttcagca gcattttttt cagctccttt tatggagaaa tcacagccgg tggcataaag
     8521 tttttcgtct attgtaattt caacaaaata ttgttttcta ttacctgacc ccacttcagc
     8581 cactacattg aattttattt ctttatgttc tttttgagcc cattcgatca gtttactttt
     8641 aaaattgttt tctaatttct ccaggtcgtc aatatcaaaa tgaacattga tgattctgtt
     8701 tattaccatt ttacgggtaa aaatatatcc tttatcaagg tatattgctc cgataagtgc
     8761 ctcaaaggca tctcccggca ttgatctaaa ataggtcttt gaatcttgag ttgatatgat
     8821 aaaatgatca attcccaatt ttaaagcaag cttattaagt tgttcgcggc ttacgatttt
     8881 ggaacgcatt tcggtaagaa acccctcttc gcgatacgga aactttttaa aaagataatc
     8941 ggcaacgata gagcttaaaa cagcatctcc aagatattcg agtctttcat tagaaatctt
     9001 atgaccactt tgttctgtag cagccgagcg gtgaataagg gctaatttat ataaagcaat
     9061 attttctggc cagaatccaa aaatattcct aagcgaaata atcagatttt tatcagaaga
     9121 gaattgcgct ttaattctgt tgtacaaaat caaattaaaa cctatttgtc ctgaaatttt
     9181 ttaagagcta tacaagtgtt atgtcctcca aaaccaaatg agtttgaaag tgcaaaattt
     9241 acttctcttt ttacagcgtt accaaatgta aaattgaggt tattatcaat atccggatcg
     9301 tcggtaaaat gattgatagt aggaggaata atactgtttt ttattgcgag tattgtagca
     9361 attaactcaa cagccgctgc tgatccaagt aaatgaccgt gcattgactt ggttgcattg
     9421 aggtttattt tataggcatg ttgcccaaac aaattaacca ctgccttttg ttcagcaatg
     9481 tcgccaactg gtgtggaggt gccatgtgta tttatatggt caacatcttc gggcttaatc
     9541 cctgcttctt caacagcaag tttcattgcc atgtatgctc caagtccgtc aggatgaggt
     9601 gatgttatat gatgagcatc ggcagtactc ccttcaccag caatttcaac ataaattttt
     9661 gctccacgtt ttatagcatg ttcgtattct tcaagaataa ttacgccgcc gccctcccca
     9721 agtacaaagc catcccggtc tttatcaaaa ggccgtgatg cagtttttgg atcatcgtta
     9781 cgggtagaaa ttgcatgcat ggcattaaaa cccccaattc ctgcggggtt aatacctgct
     9841 tcagcgccgc ctactacaaa aacactggat ttccctaatt tgatttggtt gaatgcatca
     9901 ataattgcat gacctgaaga ggcgcaagct gatgtagtag cataattagg tccacgaaaa
     9961 ccatatttaa tagaaatatg ccctgcggca atatctccaa taatttttgg aataaaaaat
    10021 ggattaaaac ggggtgttcc gtctcctttt gcaaattcaa taacttcttg aaggaatgag
    10081 gtgaatcctc caattcctgt ggctattatt acccctaccc gatctttgtc gagagtatcc
    10141 atgttaaagt ttgcatcagc gattgcctga tcggctgcaa tcatagcata ctgggtgtaa
    10201 ggatctattt ttctcgactc ttttcgatcg aagtagttgg cggggtcata accctttatc
    10261 tcacacgcaa tctgggtttt aaactttgaa gcatcgaagc gtgtaatacc agcagcgccg
    10321 cttaccccat taataagact gtcccagagt tcttcaacag tgttgcctaa tggggtaatt
    10381 gcgcctaatc ctgtaacgac aactcgtttt agattcattg cagctggatt gtttattttg
    10441 cattgttttc gatgtaggct attgcatcac caacggtgcc aattttttca gcctgatcat
    10501 caggaattgc aatattgaat tccttttcaa attccatgat tagctcaacg gtgtcgagtg
    10561 aatcagcacc gagatcattt gtgaaattgg cttgaagtgt tacttcgttt tcgtcaacac
    10621 ctaatttgtc aacgataata gctttaactc ttgttgcaat gtcagacata ttttatagtt
    10681 tttaattaga actgaggaca aaggaaatac ttttacacta tatgaccaaa atttttatac
    10741 taataaaatc gaataaactt tgttaaaacg ttgatatatc gagctttaag ctgtttttat
    10801 tcttttaaac taaaataggt atttattcta aaaaacaatt tttttcattt ttttctagtg
    10861 tttggaaaaa tttatttact ttgatttttt tcttttaaaa aatagacatt tacaatgaat
    10921 agaattgcaa tttttgcgtc aggtaatggt tcaaatgctc aaaaaatagc tgagtatttt
    10981 tcaaatatca atttagcaaa agtaaccatg atattatcca ataacccaga cgctttcgta
    11041 ttgcatcgtg ctcgtcaatt tggcgttgct actaaagttt tcgatagaaa acaattctat
    11101 cattccgaag aggttttgcg ttgtcttaaa gaaaataaga ttgatttaat agttcttgcc
    11161 ggttttttat ggttaatacc tgattatctt atcagatcgt atccaaaaaa aataataaat
    11221 attcatcctg ctttattacc aaaatatggc ggtaaaggca tgtatggcga tgctgtgcat
    11281 aaggcagtta tttccgcagg tgaatcggaa agcggtatca ctattcatta cgtaaacaag
    11341 gattatgata agggagaggt gatttttcag gccagatgta aaattcaaga aaatgacacg
    11401 gcggaagtat tagctgaaag aattcataat ttggaatacc aacattatcc taaagtgata
    11461 gaggcgattt taacagatgc cccttatcca acaaaaactg gataaaactc tacttgtttg
    11521 aaattttact ttcaaaatcc cgatatttta attaaagagt caattagccg ccatgtatct
    11581 attacacatt tggcttgatg agtataaaca aattgttatg gcctgtgttt tcaatatcat
    11641 gaactaccaa tgggattgaa tagttattaa atattactca acctgaattt agaggaaata
    11701 aatattaatg atgtgttgat tattcttttt atatttttgt tggaaaagta ttctctaaat
    11761 caatgaacct taaatgaaat ggtattaact agaaaatacg aattattgaa acctgtcaat
    11821 tggaggcaca ggattattct tattgttgac gatgttattc caaatttcaa gatattagaa
    11881 aaggctttgt tgcccaccca agcaaagatt gaacatgcga aaataggatt tgaggggata
    11941 cgaaaatgcc tggaaaatca aaaaattgat gtggtactga tagagattaa tttatcagag
    12001 ctaagtgaat tggaattagt caatcaatat agagtatttc gacctgatct tgtaattatc
    12061 gggcattctg catttgttac aactgttaca cgtcagaagt gtttggaagc tggcatggat
    12121 gattttttta ataaaccgat agattcaaaa gagctaattg taataatcgg gatggcttta
    12181 aaaaaaagag acaaaaaatt ataatacctg actctttgta ttatgttgtc aaactgtaac
    12241 aaactcatcc ttttgtttgt atctagaact tttgaaaagc acttgaattt tctaatttcc
    12301 atacctttat catacaatag actaaaacag caaatgattt aaaagaatta cttgttatta
    12361 taaaacacca ctaaagactt taaaatataa aatccaccgg ttcgtttttg tgcttataag
    12421 taaaacgtat gatgaaacgt ctcactatca tttctttggt tttatttttt ttcttcaata
    12481 ttttatcagc tcaagctcaa aatcaggttg atcctgatgg atttaatagg ttttattatc
    12541 ctaatggtca ggtgtcaagc gagggttatt tgcgtaacgg acaaccggat ggctattgga
    12601 agacttattt cgaaaatggc tctttgaaat ccgaaggaaa tcgtattgac tttttgcttg
    12661 atagcacatg gactttttat gatgaaaatg gaaacaaaac cctggaagtt aattataaag
    12721 caggaatgaa agagggcaaa aggtttacct atcgtgaaaa tgaaactatt gaggagaatt
    12781 tcagcaatga tataaaacag gggcaaacga tttattacta tctcgataat cggattttta
    12841 ggatcatcaa ttttgagaat gggcttgaag aagggatggc caaagaatat gctgacgatg
    12901 gccgtgtaat acttatcacc acttaccgaa agggttttct tattagtcgt gaaaggataa
    12961 ataggttgga tagtgaaggc aggaagcagg gacattggaa attctttcat aaaaacggat
    13021 tggtaaagct cgagggtttt tatctgaatg atttgaaaaa cggctttttt aaagaatatg
    13081 actctgcagg gaaacttgtt gaagctacta aatggcttaa aggagagata caggaacagg
    13141 ccgaggaact aactaagctt gaggtttcaa aagaatatta tcctgatggt aaaattgcag
    13201 tgtttcaaac ctttaaaaat ggtattcctc atggagtaaa acgtgattat tcaaaagagg
    13261 gaactatagt ttcaggtagc ctctataaag agggaaaact tattgcaagc ggaattacac
    13321 gagaagacgg cgtgagaaat ggagcctgga aagattatta ttctgatggg aagctcaaag
    13381 cagaaggaaa ctacaaggat gggaagatga tcgggaaatg gaacttttac catgttaatg
    13441 gaaagattga gcaaatcggc gaatttgata aacaaggccg tccggtcggc aaatggttgt
    13501 ggtattatcc atcgggaaat ttgcttcgtg aagagcatta tattggtggg aaggccgatg
    13561 gtatgatgag cgaatattcc gaaaccggcg atctaattgc cgaaggcgat tatattgatg
    13621 gtgaagaaga ggggcaatgg aagtataaca ttggtaattt caaagaagag ggtgggtatt
    13681 catatggctt acgtcacgga ttatggaagc attattataa cgatgggacg ctgatgttcc
    13741 agggtgaatt tattaatgga aagcctcatg gaaaacatac tgtattttgg gataacgaga
    13801 aaataaaaga tgaaacaact tacgaaatgg gtattaaaca aggtaactca tacagttata
    13861 actacagcgg agagttgatt cttgtaatca cgtatcaaga tggagccgaa gtctcttatg
    13921 atggaataaa agttgaccct ctgattttgg gtgaagatga gtagtgtctc tgtgtttgtt
    13981 tatccaataa atttatagat ttttcccggc agagctttta caattccatt aaactcaagg
    14041 ttgagcaatg cagcagctac tttgctcata tttaatctgc tttgtaaagt aatttcatca
    14101 attcccattt ccccatttcc acgcataaga ctaattattt ttttttcatc gggggttagg
    14161 ttaacaaaca atagatgttg tttaacattt tgttttttct tttcccatcc catcatcttt
    14221 gcaatatcag tagcactctg tattaaagcg gctttattat ccctgatgag caggttacac
    14281 ccttctgacc atgtatcacc gatcctacct ggaattgtga atacctctcg gttgtatgaa
    14341 ttggccatat cggcagttat taatgctccg ccagattttg cagcttcaat aaccaccgtt
    14401 gcatcagcca ttcctgcgat gattcgattg cgctgaggaa agttctcacg atcaggtaat
    14461 gtaccgctca tgaactcaga taataatccg ccatgatcaa ttattttagt ggccagattt
    14521 cggtttaaat gagggtaaat cctatcaaga ccatggccta acactgctat tgtatttaaa
    14581 tgattatcca gagcgctatg atgagcacaa gtatcaatac catatgctaa gccgcttata
    14641 accaacacat tgtattcagc caaatcctta accagctttt cgcaaatcat tttaccataa
    14701 tcagtagctt tgcgtgtgcc aacaatactt attatcctcg aaacattgag cgaagccgtt
    14761 cctttgtaat aaagtaaaat tggtccatcg tcgcaatttt tcaggagctc aggataattt
    14821 ttatcaaggt aaaacaacgg tgttatgttg tttttttcaa tgaaggcaat ttctttttca
    14881 gcctgccgaa aaacaaagtg atttaatatg ctattgataa gtttttcgcc gataccggga
    14941 attttttcta atgcttctct tttttgctta aaaacggcct ctacacctcc gcaataggca
    15001 ataagttttt tgcctgttac attaccaacg ccgggtaata aagttaatgc aatctgttgg
    15061 agtagcatag agcttttagt aaaaaaatta attgggtaaa agtagctaaa atctgtaatt
    15121 agcatctaat gcattattgt aataatcgtg tcattatctt tttataaaat atatctttgc
    15181 caaaaaaaat atgcttagtt acatcacttg gagtgtaaat ccggaaattt tccccaacct
    15241 attaccagtg cgatggtatg gtttattatt tgccgccgct ttcttttttg gttacttggt
    15301 tttgatcaaa atttttaaac atgaaaaact gcctgttgca cttcttgatc agttagcatt
    15361 atatgtattt attgctacta taataggagc tcgtttaggt catgtattat tttatgaacc
    15421 cgattatttt ttatccaatc cacttctgat acttaaaata tggcaaggcg ggcttgcatc
    15481 tcacggcgct gctattggga ttttgatagc aatctggctt ttttcccgaa aaaacaaaat
    15541 acactatctg tgggtactcg atcgcctggt tatcgtggtt gcgttaggcg gtttttttat
    15601 acgaatgggt aatttgatga attcagaaat ttatggtcgt gctactttat tgccatgggg
    15661 atttatcttt acctcgcagg ataatatacc ccgacatcct acacaaattt atgaagggtt
    15721 aagctatctg gctatttttg gatatctgct ttggcactat tggcgtaaaa acggaaaacc
    15781 acggcaaggt tatctttttg gaatgttttt aatacttctt tttacagtac gttttcttat
    15841 tgaatttgta aaagaaccgc aggttgactt tgaaacagac atgttgctta atatgggaca
    15901 gcttttgagt atccctttta ttattgccgg tgtaattgtt gtttttagaa aggagaggaa
    15961 acctacaaaa aataattgat agctcgaaaa agttttatct ttgccggcgt tattgattga
    16021 caaaatgggg cgttagctca gttggctaga gcgttagact ggcagtctaa aggtcatggg
    16081 ttcgattccc atacgctcca cttctaaata tttaatagct tgtaattaaa aatgttacaa
    16141 gctttttttt gttttgccaa atcacttttt cgaaaccaga acaatttcta atgtactgat
    16201 aaaatatacc tacgagtgtg gg
//
LOCUS       DAOOAI010000048        15783 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_4849_length_15783_cov_5.312754, whole genome shotgun sequence.
ACCESSION   DAOOAI010000048 DAOOAI010000000
VERSION     DAOOAI010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15783)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15783)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15783
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4849_length_15783_cov_5.312754"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(131..204)
                     /locus_tag="PK345_04905"
     tRNA            complement(131..204)
                     /locus_tag="PK345_04905"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(168..170),aa:Arg,seq:acg)
     gene            complement(317..928)
                     /locus_tag="PK345_04910"
     CDS             complement(317..928)
                     /locus_tag="PK345_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HPX34390.1"
                     /translation="MKIICIGRNYVEHARELNNQIPEEPVFFLKPDTAIIIRNRPFFY
                     PDFSSKIDYEVELVLKIKKNGRHILPEFAHTYYDEIGIGIDFTARDMQESLKTKGLPW
                     EKAKAFDGSAPISSFVNISKFNDLNDIPFSLRKNGETVQLGNSGLMIFDFNTILVHIS
                     KYITIKMGDLIFTGTPAGVGPVAIGDRLEGFLGEEKMLKVDIK"
     gene            complement(1033..2091)
                     /locus_tag="PK345_04915"
     CDS             complement(1033..2091)
                     /locus_tag="PK345_04915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPX34391.1"
                     /translation="MKRKTILFFSPNISTFIQRDIELLSSDYQVKIFNLNQGSPLKLI
                     PQLFKQLLFLIKNFRSADISICFFAGYNSVLPVIFKRCFKLPTLIIVGGTDAARFPKI
                     NYGSYVKRLNGMATAFSLRYCNHIIPVHESLVYQKYTYDDAGAPEQGYTVFAKKTKNV
                     PFTPIYFGFDSQFFKANENNRKPLTFITVGNLQHKSLYIRKGYDLIIELARKRPELSF
                     TLVGWDGKTHIDVPKNVQLLPYMNQTEVKTAFTEHEFYFQLSVMEGMPNALCEAMLCG
                     CIPIGSNVSGIPFVIGDTGYILEQRNIDKLDNLIENILSNDNRKALSKAARERIKENF
                     TYYRRQKELSEIIELYTK"
     gene            complement(2108..2929)
                     /locus_tag="PK345_04920"
     CDS             complement(2108..2929)
                     /locus_tag="PK345_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPX34392.1"
                     /translation="MELNLTKPLAFFDLETTGISVAEDRIVEISVVKVYPDGKTDVYT
                     KLINPTIPIPPKSTEIHNITDEMVKNSPTFKEIAKELNSFFGNSDLAGYNSNKFDIPL
                     LVEEFLRADTDFDLKNRKFIDVQNIFHKMEPRNLKAAYKFYCEKELSGHHSAEADAMA
                     TYEILKAQLDRYKGVKIKDKDGQTIEPIVNDVTELSAFSEQHNSADLVGHIIFNDNKQ
                     EVFNFGKHKGKPVEQIFAKEPSYYDWMMKSAFPLSTKKVITSIKLRQFNKGSVIL"
     gene            complement(3009..3281)
                     /locus_tag="PK345_04925"
                     /pseudo
     CDS             complement(3009..3281)
                     /locus_tag="PK345_04925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017140876.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
     gene            complement(3742..5712)
                     /locus_tag="PK345_04930"
     CDS             complement(3742..5712)
                     /locus_tag="PK345_04930"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPX34393.1"
                     /translation="MKLSVVIVNYNVMHFLEQCLNSVRKAVKNIESEVFVVDNNSVDG
                     SVKMVREKFPEVNLIVNPDNRGFSKANNQAIKIAKGEYVLLLNPDTVVEDDTFEKIIK
                     FMDAHEDAGGLGVKMVDGKGKFLPESKRGLPTPAVAFYKIFGLSKLFPKSKIFGQYHL
                     TYLNNDEIHEVDVLSGAFMLIRKPLLDKIGGLDESYFMYGEDIDLSYRITKEGYKNYY
                     FPKTRIIHYKGESTKKSSINYVLVFYQAMIIFAKKHFSQKNAKLFSLLINMAVYFRAF
                     LAILRRLADKVFLKLLDAVFIFAGIYFIQSYWATSVVFKDGGNYPIEFLLIAVPAYIF
                     IWLFSVYLSGGYDKPISLYKIFRGLLAGTIAILVFYSLLDETMRYSRAMILLGSFWAY
                     LAMTSIRYLLHWTGFSEFKIGEVENKRYIIIGGAEEAKRVAGLLTTIETQPAFIGLVS
                     YHDVKSQGNFIGKFEQVKEIIEIYKINEIIFCAKDVPSGSIIDKMSELKHMQVDYKIA
                     PAESLSIIGSNSINTSGDLFALELNSIDKTANRRNKRFLDIVASITLLLSLPITIFLV
                     KQPFYFILNIFQVLFARKTWVGYCNTTSDNTKRLPKLRTGVLNPASALHYKEIPDDTL
                     DRLNLLYARDYKTKNDINLIFKGIRKLDNRPN"
     gene            complement(5846..6457)
                     /locus_tag="PK345_04935"
     CDS             complement(5846..6457)
                     /locus_tag="PK345_04935"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPX34394.1"
                     /translation="MIIILIALTSIVSFLAFSRNDLFDKLQFNAWYIKHYKEWYRFFS
                     YALLHADWAHLIINMFVLWSFSSIVLSHFTIIYHGAAGLHFLLLYVGGTIFSTLYDFW
                     KNQENIYYNAVGASGAVSAVVFASIILYPNGKIFFYFIPIGIPSWLFGILYLIYSAYM
                     GKRAKDNIGHNAHFWGAIFGILYMVVTSPNTIKLFYNQLKLFQ"
     gene            complement(6480..7307)
                     /locus_tag="PK345_04940"
     CDS             complement(6480..7307)
                     /locus_tag="PK345_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34395.1"
                     /translation="MSYTLKSILFWFISFLLMASAAIYQRTTGPTYPVRGTIVIDNEE
                     IKYRLIRTWEGDTKAEVKIKVINEQILGKYRFKRFKSHDEWSEPIPMQRDGEMLVAKL
                     PNLPPAGKIQYEVFLGKENNLTLVTKKTLILRYKGVVPQWALIPHIIVMFLAMMFATR
                     TGFEALIKGKYLKNYALFTVILFGFGGFLFGPIMQKYAFGVYWAGWPFGTDLTDNKSL
                     ATFVIWLIAWLQIRKNPNRRWWVYAAVVVLFITFIIPHSTMGSEIDFTQTEAPPAIN"
     gene            complement(7422..10409)
                     /locus_tag="PK345_04945"
     CDS             complement(7422..10409)
                     /locus_tag="PK345_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HPX34396.1"
                     /translation="MKKYSVAIIFLIIISFFPASVKSQQWGISSDIAGLTEVYPEMLW
                     VDSVMNSLTVDERIAQLLMIRTYSNKDRRYYDSISRLIIQYNIGGLTFFQGSPVRQAE
                     LINYWQYLAKTPLMVSIDAEWGLAMRLDSVIPFPKHMTMGAVQDEEVIYKAGYHIGAQ
                     CKRAGIQMNFAPVMDINSNPKNPVINFRSFGEDKVNVAEKGAAFIQGMQNAGVIATAK
                     HFPGHGDTDTDSHYTLPVLNHSIATIDSADLYPFRSAIAAQTGAIMTAHLFIPAIDST
                     KNRATSLSDKAINQLMKDKMGFKGLAITDALDMDGVTNYHKPGEIELMALLAGNDILL
                     LTRNIPLALQKIKEAVVKGLISQEEIDARCRKILAYKYKTGLAHKKPVTIENLTNELN
                     SSANSWVTQQIFEKAITIVKNDHGLIPLTHLDTLKIAALSIGSIKTSPFQQRLGSYAP
                     VELFNLPSNPDKAAINEIISKLNDFNLIIVGVENTNNNLSKNYGISSSAKELIGLLKA
                     RKNKIILDVFGNPYSLSNFSKHQGIDVIICSYEDNSVSKDISAQIIFGAIGASGRLPV
                     SASSAFSVGTGVDTKPIGRLKYTLPEELGISSEKLDTITKLIESGIKQGAYPGGQVLF
                     AKNGKVFYNKAFGHHTYDRKRSIQTTDLYDLASLTKILATTPAIMQMYEMGSFNLDEP
                     LSEYLPFLKNSNKQSRTIREIMAHQAQFQSWIPFYKKTIKDFALDPTIYSSTQTKLFD
                     QQVAKNLFISNSYREIIFDSIAKSKLLPKREYKYSDLGFILLSEAVEEKTKMSFEKYC
                     EQHFYRPMGLPTIGFKPIMRFSLEKIAPTERDTTFRKQLIHGYVHDPTAAMLGGISGH
                     AGLFGNAAEVAAIMQMFLQNGYYGGQRYFSSNTMAEFTRQQFPLSQNRRGLGFDKPFP
                     VFDPNGPVSKEASLDSFGHSGFTGTYTWADPKNQLIYVFLSNRIYPDADNRKLMKMNL
                     RTKIHELMCQILNDIENSVLKRP"
     gene            complement(10590..10934)
                     /locus_tag="PK345_04950"
     CDS             complement(10590..10934)
                     /locus_tag="PK345_04950"
                     /inference="COORDINATES: protein motif:HMM:NF017007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TM2 domain-containing protein"
                     /protein_id="HPX34397.1"
                     /translation="MNALLKYLPDIDGEELFYLDNLLKGKSEEQIKQFSFIYSSRRRS
                     PMLILITTLVGFIGVAGIQRFITDQIGLGILYLLTGGLCFIGTIVDIINYRNIAFTYN
                     RKVAQEVNAMIG"
     gene            11001..11144
                     /locus_tag="PK345_04955"
     CDS             11001..11144
                     /locus_tag="PK345_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34398.1"
                     /translation="MTITAIKPKGCISIDLLMLPCKAILTDRPSPQPGHDSTPRLLNG
                     HKV"
     gene            complement(11330..12346)
                     /gene="murB"
                     /locus_tag="PK345_04960"
     CDS             complement(11330..12346)
                     /gene="murB"
                     /locus_tag="PK345_04960"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF000755.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HPX34399.1"
                     /translation="MKIGENISLKKYNSFGIDVKARYFIELTSNNDIFTFLKSHFLDD
                     KQFLILNGGSNILFTKDFNGVVIKISTQGITIIEENDNWALVKANAGENWNNFVLWCL
                     DRGLGGLENLSLIPGNVGAAPVQNIGAYGVEQKELFQSLEAVEIKSGRIVNFSNEECR
                     FGYRDSIFKHDTKNQYIILSVNYKLSKKPALNTSYGDIQKKLSSIPLADQTIKHVSDA
                     VCEIRNSKLPDPAKIGNAGSFFKNPIVNLEQLMLIKSKYPDIPVYPADNDTFKLAAGW
                     MIDKLKWKGFRYGDAGVYHSQALVLVNHGQASGSDILKLAKMIMKSVKENFGVDLEPE
                     VNIH"
     gene            complement(12585..13637)
                     /gene="porQ"
                     /locus_tag="PK345_04965"
     CDS             complement(12585..13637)
                     /gene="porQ"
                     /locus_tag="PK345_04965"
                     /inference="COORDINATES: protein motif:HMM:NF033711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system protein PorQ"
                     /protein_id="HPX34400.1"
                     /translation="MNLKSTFTISIFLFLFIGPLAKAQTNGVSVYKFLNLTSSARAAA
                     LGGNFMAIKDGDLSQVLFNPSAISKETDNKLALSFVDYYSDISYGYASYANSFEKIGS
                     YAATIQYISYGNFVYADQNGQTGGNFTASELAMVLGWGRELDSLFSIGANLKMIYSGY
                     EDYTSYGLAVDIAGSYINPEHNLTVSLTARNIGTQLKPFVSRNIESLPLEINLGVTQK
                     LKHLPFRYSIILTNLHKWDLTYYDPNDPNNKVDPFTGEPQKKDAFETFLDKAMRHVVI
                     GGELLPTKNLSLRIGYNYQRRQELGIYNKMGTVGFSWGIGLRISKFQLDYSRATYHLN
                     GSPNYITISTKLSDLF"
     gene            13996..>15783
                     /locus_tag="PK345_04970"
     CDS             13996..>15783
                     /locus_tag="PK345_04970"
                     /inference="COORDINATES: protein motif:HMM:NF012200.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor D domain-containing protein"
                     /protein_id="HPX34401.1"
                     /translation="MKNKIYIQYLWFAIFVLMATVVSAQQVDPAEDVTQISYRSVEPP
                     DYYPTRTALTKNEMHVLDTLTAAERTFIESAYDPPAIGVVRDLTDPIRFSMEQNNFLT
                     QDEISVSGGRLTRVTDDLLVFTTYFESKKADEIRIFFAEGNFPYGVEVNLFSRDDYAF
                     NQPELVGELDEHGFYTTTTFADYLYLQVVIPLDKVNDNVSFAITRITHVDNRYIPDEI
                     NRDCFLDANCSDANSFTHIDGFRKATARLSWPTGGGLVGLCTGTLLNDMRTVDFQPFL
                     LTANHCFSTQNDATGLEARFYYWSTECNSGVVNPNYIIVNGSNLIATNSQTDFTLVLL
                     KQQGGNVYMGWSANSVANNTIMHSVHHPGGTLMKYHRMLNKTSPSWTCDGFSTSNFHY
                     TKVTHGQEAGGSSGGGIVDPDGRIRGQLYGTCHLEVWDPCDYDTYYNMWGNFGVTYSN
                     NNIQYWLANGGASVSMYVVNSINFGYVNVGNYNDYNLAIKNNGTRPNFLNLEINNAYI
                     TGTNSSHFSIIGSKILYLAPGETGYITVRYKPLESGSSVATLNLVHNADNYTTPRQVT
                     LNGSSSPCSDIISLGGGGYANRKTFSKSGD"
BASE COUNT         4620 a         2910 c         3101 g         5152 t
ORIGIN      
        1 ttttttatca acagtttatt tttgataaac aggacttaaa catgttttta aggattgact
       61 aactatgaaa agagagcatt gtgaataaaa aagcagttaa caaaaacatg ctaactgctt
      121 tgtatttagg tgactccgaa gggactcgaa ccccaaacct tctgatccgt agtcagatgc
      181 tctatccaat tgagctacgg agccatccgt tataaaaacg gctgcaaagt taaacaatat
      241 tgatttgtga tgtgtaatta aatggctgaa tttttataaa actgtttaga agatttcccg
      301 gcgccaactt ttgatcctat tttatatcaa cttttaacat cttttcttct ccaagaaatc
      361 cttccaggcg atcgccaata gccacagggc caacaccagc cggggtgccg gtaaaaatca
      421 ggtcgcccat ctttattgtt atatatttag agatgtggac aagaatcgta ttgaaatcga
      481 aaatcattag cccggaattt cccagttgaa ctgtttcgcc attttttctc aatgagaaag
      541 ggatgtcatt taaatcattg aatttagaga tgttaacgaa gctgcttatt ggggctgatc
      601 catcaaatgc ttttgctttt tcccatggga ggccttttgt tttaagcgat tcctgcatat
      661 ctctggcggt aaaatcaatg cctataccta tctcatcata atatgtatga gcaaattcag
      721 gtaatatgtg ccggccattt tttttgattt tcaatactaa ttcaacttcg taatctattt
      781 tggaagagaa atcaggatag aaaaaaggcc gattgcgaat gattatggca gtatcaggtt
      841 taagaaaaaa aaccggctct tcgggtattt gattatttaa ttcgcgggca tgctcaacat
      901 agttgcggcc tatacaaatt atcttcataa acaaggtgtt taataatacc ggcaaattta
      961 aaatttaccg gaaaaataat cagagattaa atgagtaaga cgtaatccca tgattgtcaa
     1021 tcactattta tattattttg tgtataactc tataatttct gagagttcct tttgccgccg
     1081 atagtaggta aagttttctt taattcgttc tcttgcagct tttgatagcg cctttcgatt
     1141 atcgttactc aaaatatttt cgataaggtt atctaattta tcaatatttc gctgttcaag
     1201 gatgtagcct gtatcaccaa ttacaaatgg tatgcccgac acattggagc caataggaat
     1261 gcatccgcac agcattgctt cgcaaagagc atttggcatc ccctccatta ccgaaagctg
     1321 gaaatagaat tcgtgttcgg tgaatgccgt ttttacctca gtttgattca tgtatggcaa
     1381 cagttgtaca tttttaggaa cgtcaatatg tgttttgcca tcccacccaa caagtgtaaa
     1441 tgataattcg ggtcgtttgc gggcaagttc gataatcaga tcataacctt tcctgatgta
     1501 aagagactta tgttgcagat taccaactgt aataaaggtg aggggcttcc tgttgttttc
     1561 gtttgctttg aagaattggg aatcaaaacc aaaataaata ggtgtgaaag ggacgttttt
     1621 agtttttttg gcaaatactg tatagccctg ctccggagcg ccggcatcat cataagtgta
     1681 tttttgataa acaaggcttt cgtgtaccgg aattatatgg ttgcaatatc gaagcgaaaa
     1741 ggctgtggcc atcccattta atcttttaac atagcttcca taattgatct ttggaaatcg
     1801 ggcagcatca gttccaccaa ctatgataag tgttggcagt ttgaaacacc tcttaaaaat
     1861 aacaggcagt actgaattgt atcccgcaaa gaaacagata cttatgtcgg ctgagcggaa
     1921 atttttaatc agaaacagca gttgtttaaa aagttgtggg attaatttta gcgggcttcc
     1981 ctgattcagg ttgaagattt ttacctgata atcggaggac aaaagttcaa tatctctttg
     2041 aataaatgta gagatgtttg gggaaaagaa taaaatggtc ttacgtttca tttttaatag
     2101 gttatgttta caggataact gatcctttgt tgaactgtct tagttttatg gatgtgatta
     2161 cttttttggt cgaaagggga aaggctgatt tcatcatcca gtcgtaatat gagggctcct
     2221 tagcaaaaat ctgctcaaca ggtttacctt tatgttttcc aaaattaaat acttcctgct
     2281 tattgtcatt aaaaataata tggcctacca gatcggcaga gttatgttgt tcggaaaaag
     2341 cgctaagctc tgttacatca tttacaatag gttcaatggt ttgcccgtct ttatctttta
     2401 tcttaacccc tttgtatctg tcgagttgtg ctttcaggat ttcgtatgta gccattgcat
     2461 cggcctctgc cgaatgatgg ccggaaagct ctttttcaca ataaaatttg tatgcagctt
     2521 ttaggttgcg aggttccatt ttatggaaaa tattttgtac gtcaataaat tttcgatttt
     2581 tcagatcgaa atcggtgtca gcccttaaaa actcttcaac aagcaaggga atatcgaatt
     2641 tgttcgagtt gtatcctgcc agatcgctat ttccaaaaaa actattcaat tccttggcaa
     2701 tctctttaaa agtcggtgag ttttttacca tttcatcggt aatgttgtgt atctcagtag
     2761 attttggagg gatcggaata gtggggttta tcagttttgt ataaacatct gttttgccat
     2821 ccggataaac ttttactacg cttatttcta caatacgatc ctctgcaacg ctaataccgg
     2881 tagtttccag gtcgaagaaa gctaaaggtt tagtaagatt tagttccatg taattttcaa
     2941 taaaagaaaa cccctgaatt tctcaattag aggtgttaag tttatgatag gtataatatt
     3001 ttattttctt aaacatcttg attcagatca aaattctcaa ggcgttcttt aatttgtttc
     3061 agatacattt cgcccggtgc gccatccact attctatggt cgtatgctaa tgaaattatc
     3121 atagtatgcc gaatggctat tacatcgcct tgactggttt caagtacttc aggtcgtttt
     3181 tttattgcgc ccgatctgag gatagccact tgtggctgat ttatgactgg agtacctgta
     3241 aggctgtcga agctttcaaa attggttacg acgaacgctc cataaagttc ggattctact
     3301 gcctcaaatc tgttagtgga cctgtatttt aaatagttaa ggatatcatc cttggttaag
     3361 tggttgccct taccgttact ttctattttc tccagtttct gtaaggcgat attcactttt
     3421 ttagcgatac ttttaactaa aggtgagtag aagcgtgtgg gggttgctaa atcatcacta
     3481 atcaaacacc ctgtttctgt tggaacatga tatgttatgt caggcttaat atcagtgtca
     3541 ggttttttta gccattttat gttggtggct tcgataatgc tttcacccat tttaggcatt
     3601 attatttcga aaattgccat ttaactttac acttcaatat caaattaata acgctttcga
     3661 aacaaagtga tatagtaaat gtttatgaat ataattgacc gcaaaactaa tgatttagca
     3721 attaatattt ttcaggtctt actaattcgg gcgattgtca agttttcgta tgcctttaaa
     3781 gattaggtta atgtcatttt ttgttttata atctctggca tacagtaagt taagtcggtc
     3841 gagtgtatca tcaggtatct ctttataatg tagcgccgag gctgggttca atactcccgt
     3901 gcgtagctta ggcagacgtt ttgtgttatc cgatgtagta ttacaataac caacccatgt
     3961 ttttctggca aagagtactt gaaaaatatt taaaataaaa taaaaaggct gtttgacgag
     4021 aaaaattgtt atcggcaggc ttagcagtag ggtgatggat gcgacgatat caagaaatct
     4081 tttattgcgg cgattggcgg ttttatcaat ggagtttaat tccaaagcaa ataggtcgcc
     4141 ggaggtgttg atggagttac tgcctattat tgaaaggctt tcggcaggag cgatcttata
     4201 gtcaacctgc atgtgtttta actccgacat tttatcaatt atactacctg aaggaacatc
     4261 ctttgcgcaa aagataattt cgttaatctt gtagatctct attatttctt taacctgttc
     4321 aaattttccg ataaagttac cctgcgattt aacatcatga taagatacca atccgatgaa
     4381 ggcaggttgt gtttcaattg tggtaagtag cccggctact cttttagcct cttcggcacc
     4441 gccaataatg atatatcttt tgttttctac ttctccgatt ttaaattccg aaaaccctgt
     4501 ccaatgtaac agataacgga ttgaggtcat tgcgaggtaa gcccaaaatg agcctaacaa
     4561 tatcattgca cgggaatagc gcattgtttc atccaaaagg ctgtagaata ccagaatagc
     4621 tattgtgccg gcaagcaacc ctctgaatat tttataaaga ctgattggct tatcgtaacc
     4681 accggaaaga tagacagaaa aaagccaaat gaagatataa gcaggcacgg caattaataa
     4741 aaactcaata ggatagttgc cgccatcctt aaatactacc gaagtagccc aatagctctg
     4801 gataaaatag atacctgcaa aaatgaaaac ggcatcaagc agttttaaaa ataccttatc
     4861 ggccaaacga cgcagaatag ctaagaaggc tctgaaatat acagccatat tgatcaatag
     4921 agaaaagagc ttggcgtttt tttgtgaaaa atgttttttt gcaaaaatga tcatggcttg
     4981 gtaaaagaca agtacgtagt ttatcgagct ctttttggta ctctctcctt tgtaatgtat
     5041 gatgcgggtt ttaggaaaat agtagttttt atagccctct tttgtaattc ggtagcttag
     5101 gtcaatatct tcgccgtaca tgaaataaga ttcatcaagc ccgccaattt tgtcaagaag
     5161 tggttttctt attagcatga atgcgccact aagtacgtcc acttcgtgaa tttcgtcatt
     5221 gttgaggtag gtgaggtgat attgcccgaa aattttggat ttcgggaaaa gctttgaaag
     5281 cccgaagatc ttgtaaaagg ccactgcggg agttggaagg ccacgcttgg attcaggtaa
     5341 aaattttcct ttaccatcca ccattttcac tccgaggccg ccagcatcct catgcgcatc
     5401 catgaacttt attatctttt caaaagtgtc atcttcaacc actgtatcag gattaagtaa
     5461 aagtacatat tctccttttg ctattttgat ggcctgattg ttagctttgg aaaaaccacg
     5521 gttgtcagga ttgacaataa gatttacctc aggaaatttt tccctaacca ttttcaccga
     5581 cccgtcaacg gagttattgt caacaacaaa tacttcactt tcaatgtttt ttacagcttt
     5641 acgaactgag ttaaggcact gttccaaaaa gtgcatcaca ttataattga caataactac
     5701 tgatagtttc atgaaaacga atttttataa atcgccattt gagccgtcaa agatagagag
     5761 tttataaaac tggtgggaga tagcagagga taaaagtaaa taaaggtaaa cacaatttgt
     5821 tttttatgct gttcggaaaa accgattact gaaaaagttt caattggttg taaaaaagct
     5881 taatggtgtt cggggatgta accaccatat ataaaattcc aaaaattgct ccccaaaaat
     5941 gcgcattgtg cccgatatta tcctttgccc gtttgcccat gtaagccgaa tagataagat
     6001 ataaaattcc gaaaagccat gatggaatac ctattggtat gaaatagaaa aatatttttc
     6061 cgtttggata aagtataatg cttgcaaaca ccactgccga tacagctccg gaagcgccaa
     6121 ctgcattgta atatatattt tcctgatttt tccagaaatc atatagagtg gaaaaaattg
     6181 tacctccaac atacaggagt aggaaatgga ggcctgcagc gccatggtaa attattgtga
     6241 aatgactaag cacaatgcta ctgaacgacc acaatacaaa catattgatg attagatgcg
     6301 cccaatcagc atgaaggaga gcataggaaa aaaatctgta ccactcctta tagtgtttaa
     6361 tgtaccaggc attgaactgc aatttatcaa acaaatcgtt tcggctgaaa gccaaaaagg
     6421 aaacaatact tgtgagcgct attagaatta taatcattgc gtcggtttgt aattgtcaat
     6481 caattaattg cagggggcgc ttctgtttga gtaaaatcaa tttctgatcc cattgtgctg
     6541 tgaggaatta taaacgtgat aaacagtaca acaacagcag catataccca ccagcgccta
     6601 tttggatttt tcctgatctg tagccatgct atcagccaga ttacgaatgt agccagagac
     6661 ttgttatcgg tcaggtctgt tccaaacggc cagcctgccc agtaaacacc aaaagcatat
     6721 ttttgcatta taggtccgaa taaaaagcct ccaaaaccaa acagaattac agtaaacaaa
     6781 gcatagtttt tcaggtattt gcctttgata agcgcttcga accctgtacg tgttgcaaac
     6841 atcatagcca aaaacattac gataatatga ggtattaatg cccattgggg aactactcct
     6901 ttgtatcgca gaatgagcgt ttttttagtt accaacgtaa gattgttttc tttgccaaga
     6961 aatacttcat attgaatttt cccggcaggc ggcaggttgg gaagctttgc cacaagcatt
     7021 tcaccatccc gttgcatggg tattggttcc gaccattcgt catggctttt aaatctttta
     7081 aatctgtatt ttccgaggat ttgttcatta ataactttga ttttcacctc tgctttagtg
     7141 tcgccctccc acgttcttat aaggcgatat ttaatctctt cgttgtcaat aactattgta
     7201 cctctaaccg gataagtggg gcctgtagta cgctggtaaa tagctgcaga ggccatcagt
     7261 agaaaagaga taaaccagaa aagtatagat tttaatgtat aactcatcac tgtttaattt
     7321 ttactttaca aaataatgta acagaagcga ttaatacaaa aaattaaata tttaaacatt
     7381 gtaaaaaatc tttttatgcc ataatttttg tataaaatat tttatggcct ttttaataca
     7441 gaattttcaa tatcgttcaa aatctgacac attaactcat gaatttttgt tctcagattc
     7501 attttcataa gttttcggtt atcagcatcg ggatagatcc ggtttgatag gaatacataa
     7561 atcaattgat tttttggatc agcccaggtg tatgttcctg taaaacctga atggccaaag
     7621 ctatccagtg aggcctcttt gcttaccggc ccgtttgggt cgaatacagg aaatggtttg
     7681 tcgaagccta agccgcgcct gttttgagat agaggaaatt gttggcgagt gaattcggcc
     7741 atggtatttg aactgaaata gcgttggcct ccataatatc cattttggag gaacatttgc
     7801 attattgccg ccacctctgc tgcatttcca aacagccctg catgccctga tatcccacct
     7861 aacattgctg ctgtcggatc gtgaacgtaa ccgtggataa gttgcttccg aaaagttgta
     7921 tctcgttctg tcggggcaat tttttctaaa gaaaaacgca tgataggctt gaaccctatg
     7981 gtaggcaagc ccataggacg ataaaagtgc tgttcgcaat atttttcaaa actcatcttt
     8041 gttttttctt ccacagcttc actcagaagg ataaaaccca gatcgctgta tttatattcc
     8101 cgcttcggca aaagtttgga ttttgcaata gaatcaaata ttatttcccg gtacgaatta
     8161 ctgataaata ggttcttagc cacctgctga tcgaatagtt tcgtttgtgt ggaagagtaa
     8221 attgtaggat cgagtgcgaa atccttgatt gtttttttat aaaatggtat ccaactttgg
     8281 aactgtgcct gatgtgccat tatttccctg atggttcggg attgcttgtt ggaatttttt
     8341 aaaaagggga gatattctga caatggctca tcgagattaa aacttcccat ctcgtacatc
     8401 tgcattattg ccggcgtagt tgcgagtatt ttggtaagcg aggcaaggtc gtacaagtcg
     8461 gttgtttgaa tgctgcgttt tctgtcgtaa gtgtggtggc caaaggcttt gttgtagaac
     8521 acctttccat tttttgcaaa taaaacctga ccccctggat atgctccctg tttgataccg
     8581 ctttcgataa gcttggtaat agtatcgagt ttttcagaac tgatacctaa ctcttcaggt
     8641 agagtatatt tcagcctgcc gataggtttt gtgtcaactc ctgttccaac cgaaaaagcg
     8701 gatgaggccg aaacaggaag cctgcccgag gcgcctatag ctccgaaaat gatttgagcg
     8761 ctaatgtctt tggaaacaga gttgtcctcg taagaacaaa tgattacatc aataccttga
     8821 tgtttagaaa agtttgatag tgaataagga ttcccaaaaa catcgagtat gattttgttt
     8881 tttcgggctt ttaacaagcc gattagctct tttgctgatg atgaaatacc ataattcttt
     8941 gacagattgt tatttgtatt ttcaactcca acaattatga ggttgaaatc attaagtttt
     9001 gaaattattt catttatggc agctttatct gggtttgatg gcagattgaa cagttccacc
     9061 ggcgcatagc ttccgagcct ttgttgaaaa gggcttgtct taatactccc aatggaaagg
     9121 gctgcgattt ttaatgtatc aagatgggtt aatggaatta gcccatgatc attttttact
     9181 atcgttatgg ctttttcaaa tatttgttga gtaacccaac tattggcgct actattaagt
     9241 tcattggtaa gattttcgat ggttacaggc tttttatgag caagtccggt tttatactta
     9301 taagccagta ttttgcggca tcgtgcatca atttcttctt gtgaaattag ccctttaact
     9361 acagcctctt taattttctg taaagcgagg gggatatttc ttgtaagcag taagatatcg
     9421 tttccggcga gcaacgccat aagttcaatt tcaccaggtt tatgatagtt agttacacca
     9481 tccatatcca atgcgtcagt aattgcaagc cctttaaatc ccattttgtc cttcatgagc
     9541 tgatttattg ctttatcaga taatgaggtg gctctgtttt ttgttgaatc aattgcgggg
     9601 ataaagaggt gcgctgtcat tattgctcct gtttgagcag ctatggcgct tctgaacgga
     9661 tacagatcgg cgctgtcaat agttgctatt gagtgattta gcactgggag ggtataatgc
     9721 gaatcggtat cggtatcgcc atgaccgggg aagtgttttg cggtagcaat aactcctgcg
     9781 ttttgcatac cctgaatgaa tgctgcgcct ttttctgcca cattaacttt gtcttcgccg
     9841 aaagagcgaa agttgattac agggttctta gggttactgt tgatatccat aactggggcg
     9901 aagttcattt gtatccctgc ccttttgcac tgtgcgccaa tatggtatcc ggctttgtaa
     9961 atcacttctt catcttgaac ggctcccatt gtcatgtgtt tggggaatgg aatgacgctg
    10021 tctaagcgca tggccaaacc ccattcggca tctatgctaa ccataagcgg cgtcttggct
    10081 aaatactgcc aataattgat caattcggcc tgcctgacag ggctgccctg aaaaaatgta
    10141 agtcccccga tgttatattg aattataagc cggctgatgc tgtcataata tcgcctatcc
    10201 ttattggaat atgttctgat cattaatagc tgggctattc gttcatcaac ggtaagcgaa
    10261 ttcattaccg aatcaaccca tagcatttca ggataaacct cagttaatcc agcgatatca
    10321 gaagatatac cccattgttg agatttaacg cttgccggaa aaaaactaat tattattaaa
    10381 aaaatgattg caacactata tttcttcatt ttcactaata aagtactttt tttaattttt
    10441 caggaaatac aaaactaatc ggcgtcaaag atactatgac gccggattca ttttcggata
    10501 ttttttaagt atattatgaa atatggccaa cgattgaagc taagtgccaa cagctcaggt
    10561 ggttaattta ctctctgtta tatttaacct tatccgatca ttgcattaac ctcttgtgct
    10621 acttttcggt tgtatgtaaa cgcgatattc cggtaattta taatgtcaac aatagttccg
    10681 ataaaacata gtccaccggt aagtaagtat aaaattccta atccgatttg gtcggtaatg
    10741 aatctttgta ttccggcaac tccaataaaa ccgacgagcg tagtaattaa tatcagcatt
    10801 ggcgagcgcc ggcgagaact gtaaataaaa gagaattgtt tgatttgttc ttcacttttg
    10861 cctttcagca ggttgtcaag gtagaataat tcttcgccat ctatatctgg cagatatttc
    10921 agcagtgcat tcatgatgtg gttgtttttg ggtgtttaaa atgaaaaaaa ttaatgctta
    10981 aaaatactat tctttgaaaa atgacaataa cagctatcaa accaaaagga tgcatctcta
    11041 ttgatttgct gatgttgcca tgtaaagcca tacttactga ccggccgagc ccgcagccgg
    11101 ggcacgattc aacaccaagg ttactcaacg gacataaggt gtagtgatgc ccattaatag
    11161 gcgatagaaa aagaaaaagt agagcgccaa gccatataat caattctaaa ttgaaataga
    11221 gattctttga tatttgttta aaaaggtaca tcttatttag aaaagcgaat ttaaaatatc
    11281 gaatttcaaa agtaaaataa atttgtaaag caatttaaaa atcagggaat taatggatgt
    11341 ttacttctgg ttcgaggtca actccaaagt tctcttttac tgatttcata atcatcttgg
    11401 ctaacttaag gatatcgctg cctgaagctt gcccgtgatt gaccaatact aatgcctgcg
    11461 aatgatatac cccagcatcg ccatagcgaa accctttcca ctttagttta tctatcatcc
    11521 atccggctgc taatttaaac gtatcattgt cagcaggata aacagggata tccggatatt
    11581 ttgatttaat cagcatcaat tgctcaaggt tcactatcgg atttttgaag aaactaccag
    11641 cgttgccgat ttttgccgga tcggggagct ttgagtttct tatttcgcaa acggcatccg
    11701 acacatgttt tatagtttga tcagccaaag ggattgaaga gagctttttt tggatatctc
    11761 cgtaagaggt atttagcgct ggttttttgc ttaatttgta atttactgac aggatgatat
    11821 attggttttt agtgtcatgt ttgaaaatgc tatctcgata accaaaccga cactcttcat
    11881 ttgagaaatt gacgatccgc ccactcttta tttcaacagc ttccaaagac tggaatagct
    11941 ctttttgctc tactccatag gctcctatat tttgaaccgg tgcagctcct acgttgccag
    12001 ggattagtga aagattttct aaaccgccta acccccgatc caaacaccat aaaacaaaat
    12061 tattccagtt ctcaccggca ttagctttta ccaatgccca attatcgttt tcttcaataa
    12121 tagtaattcc ttgggtagat atcttgatca caactccatt gaagtcctta gtaaagagta
    12181 tgttgctccc tccgtttaag ataagaaatt gtttatcatc caaaaaatga cttttcaaaa
    12241 acgtgaaaat atcattgttg ctcgttagtt cgataaaata tctggctttg acgtcaatac
    12301 cgaaggagtt atacttttta agactaatat tttcaccgat tttcatgttt gtaactttgg
    12361 ttgaaagcta atcatcaaaa atattattat ttgccattac gcattactat ttattcagct
    12421 tcacatcttt tgcttttaaa atggtagtat ttttttaaag ggagtaaatg agaaaaagca
    12481 tctaatacag accgttagtg aaaactgaag atttcgatat tagtacttgg tgttagcgtg
    12541 ccaactttgt ttttcggtat tttaattttg tcttttctgt gtttttagaa cagatctgat
    12601 agctttgtgc tgattgtaat atagttaggc gatccgttga ggtgatatgt cgcacgagag
    12661 taatcaagct ggaattttga aattctaaga cctatacccc atgaaaaacc tactgttccc
    12721 attttattat aaatgccaag ctcttgtcgt ctttggtaat tataaccgat tcttaaagag
    12781 aggtttttgg tagggagcaa ctcaccgcct attactacat ggcgcatagc tttatccagg
    12841 aaggtttcga aagcgtcttt tttttgtggc tcgccggtaa aggggtcaac tttattgttt
    12901 gggtcgttgg ggtcgtaata agttaagtcc catttatgca ggttggtaag tattattgag
    12961 tatctaaaag gtaaatgttt gagtttctgc gtaacgccta aattgatttc gagtggcaat
    13021 gactcgatat tacggcttac aaaaggtttt aattgtgtgc ctatatttcg tgctgttagc
    13081 gaaacggtca ggttgtgttc aggattgatg tagctgccgg caatgtccac tgcaaggcca
    13141 taactggtat aatcctcgta acctgaatag atcattttga ggtttgcgcc aatagaaaat
    13201 agtgagtcaa gttctctgcc ccagcctaag accatcgcca gctcactggc tgtgaaattt
    13261 ccaccagttt gtccgttttg atcggcatat acaaagttgc cgtaactgat atattgaatt
    13321 gtagcagcgt aggaccctat tttttcaaaa gaattggcgt aactggcata accgtaactt
    13381 atatcggaat agtaatccac gaagctaagt gcaagtttgt tatcagtctc tttcgagatg
    13441 gctgaaggat taaatagaac ttgcgatagg tctccatctt tgattgccat gaagttgcca
    13501 ccgagcgctg cagcgcgggc cgaactggtc agattgagga atttgtaaac acttacgcca
    13561 tttgtctgag ctttggcaag aggtccaata aacaggaaca aaaatattga aatagtaaaa
    13621 gtagatttca gattcacaat aatatccgtg atatgattaa taatttgaca aacgatagca
    13681 gactttttaa attgtttttg tttgcaaata gatgaagtac ataataataa ggttgcctgc
    13741 cgtgcgattt catatacaat taatagaatt aatcaacttt ttgttcacct cgaccaaaat
    13801 tagtttaaaa agtgatcaat agaaggccat tttgagctaa actgctgatt ttaatatgcc
    13861 agaaagttta aaactgaaac aattgtttgt gattcaaata ttatttattt ttgtattttt
    13921 gatttttata atttaagaat ctaatctttc tttgacatca atttgcagtt atattaaata
    13981 tttaaattct ttaacatgaa aaacaaaatt tacattcagt atttatggtt cgccattttt
    14041 gttttgatgg ctaccgtagt ttctgcgcag caagtagatc ctgctgagga tgttacccaa
    14101 atttcttatc gttctgtaga gcctcctgac tattatccta ctcgcacggc tcttactaaa
    14161 aacgaaatgc acgtacttga tacgttgact gctgctgaac gcacgttcat agagtctgct
    14221 tatgacccgc cagccattgg cgttgtcagg gatttaactg atcctatacg atttagtatg
    14281 gaacaaaata attttctaac acaagatgag atttccgttt cgggtggtcg cttaacgcgt
    14341 gttactgacg atttgctggt ttttaccacc tattttgaat cgaagaaggc tgacgagata
    14401 aggattttct tcgccgaagg taattttccc tatggcgtag aggtaaactt gttcagccgg
    14461 gatgactatg cctttaacca acccgaactt gttggagagc tggatgaaca cggcttttac
    14521 actaccacca catttgcgga ttatctctat ttgcaagttg taattccgct tgataaggta
    14581 aacgataatg taagctttgc cataacaagg ataacccatg ttgacaacag atacattccc
    14641 gatgaaataa acagagattg ttttttggat gccaattgtt ctgatgccaa cagttttact
    14701 catattgacg gattcaggaa agctactgcc aggttgagtt ggcctactgg tggtggtttg
    14761 gttggtttgt gtaccggcac actacttaac gacatgcgta cagttgattt tcagcccttt
    14821 ttactcactg ccaaccattg ttttagtaca caaaatgatg caacgggtct tgaggctcgt
    14881 ttttattact ggtcaaccga atgtaatagc ggtgtggtaa atcccaatta tattattgta
    14941 aatggttcaa atttgattgc cacaaatagt cagacagatt tcactttagt tttattaaaa
    15001 caacaaggcg ggaatgttta tatgggctgg agcgcaaaca gtgtggcaaa taatacaatt
    15061 atgcactcgg ttcatcaccc cggtggtaca ttaatgaaat accaccgaat gctgaataaa
    15121 acttctccca gttggacatg cgatgggttt agtacttcta atttccatta tacaaaggtt
    15181 actcacggac aggaagcggg aggtagctca ggtggcggaa ttgttgatcc ggacggcaga
    15241 atccgtggcc agctttatgg aacatgtcac ttggaagtat gggatccttg tgattacgat
    15301 acttactata atatgtgggg caattttggt gttacctact ctaacaacaa cattcaatac
    15361 tggcttgcta acggtggcgc tagcgtatca atgtatgtag ttaatagtat aaattttggt
    15421 tatgtaaatg ttggaaatta taatgattat aatttagcta taaagaataa cggtacccgt
    15481 ccgaattttc ttaatcttga aattaataat gcctatatta ccggtaccaa ttcaagtcat
    15541 ttttccatta ttggttcaaa gattttgtat ttggctcccg gagagacagg gtatattacc
    15601 gttcgttata agccgcttga gtcaggaagt agtgttgcaa ccctcaattt ggttcataac
    15661 gccgacaact atactacccc ccgacaagtt actctcaacg gtagctcaag cccatgttcc
    15721 gatatcattt cacttggtgg agggggctat gctaatcgta agactttttc caaaagcgga
    15781 gat
//
LOCUS       DAOOAI010000049        15660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_4899_length_15660_cov_4.680487, whole genome shotgun sequence.
ACCESSION   DAOOAI010000049 DAOOAI010000000
VERSION     DAOOAI010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15660
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4899_length_15660_cov_4.680487"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..550
                     /locus_tag="PK345_04975"
     CDS             <1..550
                     /locus_tag="PK345_04975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015546699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HPX34402.1"
                     /translation="ISKKENLPPFVIFQDPSLEDMAIQYPITMDELTKITGVGVGKAQ
                     KYGKPFLDFIKKYVDENEIVRPNDMVVKSVINKSGLKVYIIKSIDRKLPLEDIAFTKD
                     LSFDELLTELESVVASGTKIDINYYINEVVDPYHQEEIIEYFQEAESDSVEKALLELG
                     EDEFNEYEIRLMRIKFMSDVGN"
     gene            627..1031
                     /locus_tag="PK345_04980"
     CDS             627..1031
                     /locus_tag="PK345_04980"
                     /inference="COORDINATES: protein motif:HMM:NF025494.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4296 domain-containing protein"
                     /protein_id="HPX34403.1"
                     /translation="MTNKLFVFFLTLALVAGCGPVEENKSKKIPQNILNLDKMAELIA
                     DLQIAESVLNELQRNGEYDKSKAATLMSSIFETHGITQQAFDESRLFYENNPHLYELV
                     YEKVITLLTEKQTELTVKAQKDSVLRKMPLPQ"
     gene            complement(1050..2510)
                     /gene="amrB"
                     /locus_tag="PK345_04985"
     CDS             complement(1050..2510)
                     /gene="amrB"
                     /locus_tag="PK345_04985"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HPX34404.1"
                     /translation="MPGNLFFKKDKPSDRFHKSLDRQPCVAGQFYPASAKELKADLKS
                     YFNVAAPPRVGSPIAILAPHAGYIYSGAVAASAFNQIDPHSEFDRIFLIGSSHRVSFD
                     GASVYNQGDYLTPLGKVEVDIDLANKLISEHERFTYNPAADLKEHSLEVQLPFLQYHL
                     QKKFKIVPIIISTQSLHVLEQISSALEPFFNPDNLFVISSDFSHYPKYDDAKRADQII
                     AEAISTNSPKTFLSAIKKVEQSAIHGLATAMCGWSSMLVLLNISSKDKNIEIIPIEYK
                     NSGDALYGDKKGVVGYWAIAFYQKSKPATFTEQEKEMLISMAKNSIKEAILGVATEGT
                     TPDFKSEALNSQNGVFVSVYVNGQLRGCLGRFNNTEPLWQAVKQLAVSAATEDSRFDE
                     ITAEELDLLSVEISVLTPLQKIKSSKEIQLGKHGIFIKKGTNNGVFLPQVADNYHWTV
                     EEFLGHCARDKAKIGWDGWREAEIYIFEATIFGDLS"
     gene            complement(2640..5231)
                     /locus_tag="PK345_04990"
     CDS             complement(2640..5231)
                     /locus_tag="PK345_04990"
                     /inference="COORDINATES: protein motif:HMM:TIGR04131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated C-terminal
                     domain-containing protein"
                     /protein_id="HPX34405.1"
                     /translation="MRKILFFLSFFFLSLAVLGTHQRAGEITYTAISDLKYEFKIVTY
                     TYTPSPADRPELPINWGDGSSSIVQRTKKIDLPNNISRNEYDGIVHTFPGQGSYTISL
                     EDPNRNYGVLNIPNSVNVPFFLQTELVINPFLGPNNSVQLLAAPIDKGCVNSIYMHNP
                     AAYDIDGDSLSYKLTICRGEGGLPIPGYIFPNQVNTQIPTTFEIDPFSGTIIWDKPLM
                     QGEYNIAFLIEEWRYGVRIGYVTRDMQIAITACSNQPPIISPLQDDCVVVGETLSFTV
                     SATDPDNDWLSLHALGGPLILPENPASFPSASGKGSVQSTFTWTPACSHVQLQPYQIV
                     FTAKDSLNFPQLVDIKTVNIEVISPGPENLSASSVGNSIHLSWQKALCTQALKYEIYR
                     RNGLYGYQPQHCETGVPNYTGYQKIGETLNISDTTFIDTNNGTGLIHGIDYCYMVVTL
                     FSDGGKSIASQEACASLKRDIPIITNVSVEVTSQSAGEIFVAWSKPTELDTILYPAPY
                     HYIIQRATTDSPNSFSVCDSTLSINDTIFIDKELNTTKNSYLYKVDLYSEPFTNRVFI
                     GSTHTAQSIFLKIEPSDRTLTLNWDVNVPWDNDFYLIFRQNPETLEYDSIGLSANNNY
                     RDSDLINGENYTYFVRSTGYYSIQSIVSPIINFSQIVSETPIDIDPPCAQLLETTTNC
                     DAVENILTWYNPSGCPTDIAKYLIFYSPQQEGELVLLDSIDDPFTTNYTHKNLFSIAG
                     CYGVIAVDSAGNQSQMSNIQCVDISECGTYRLPNVFTPNDDTYNDRFKAFEFTSVERI
                     NLIIFNRWGRIVYQTTDPWFQWDGKNQQNNRNCSEGVYFYVCDVFEIQLEGLTKRTIK
                     GSITLLR"
     gene            5500..7716
                     /locus_tag="PK345_04995"
     CDS             5500..7716
                     /locus_tag="PK345_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0015969 - guanosine tetraphosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RelA/SpoT family protein"
                     /protein_id="HPX34406.1"
                     /translation="MYSEEEHNEISNKYNELIGCWKAQPDKGELEMINKAFDLAVHVH
                     STMRRRSKEPYVYHPIEVAIICAKDMRLDAISIACALLHDVVEDTEVTIEDIQTIFNQ
                     QIATIISGLTKIKDVLDDSTRSMQAEYFKQIILSLSNDMRVIMIKMADRLHNMRTLEH
                     MSRDKQLKIASETLMLYAPFAYRMGLYNIKTELEDLSLKYTEPEFYNHIYKQLENTEP
                     NRSKFIMQFVKPIEEILSKTEIKHQIQVRTKSLYGIYQKMSQQNISFDEVYDIFAIRI
                     ILDVDIEQERLECWKVYSLITEIYKPKHDRLRDWISIPKANGYAALHTTVMSKTGKWV
                     EVQIKSNRMHEIAETGFASHHYYKNITDENKVIEEWLNRIRDQFQNADNDALSFLDDF
                     KLDIYSDEIYTFTPKGDMIKLPVNSSALDFAYAIHTEIGNRSIGAKVNLTLVPLKHTL
                     TSGDQVEIITSKKQTPLPEWLNYVITTKAKYHIKEALKIEKKKHYSDGKQKLNELFET
                     VGIEFSKQNIKKLKDFIKIESLIDLYYEADVGRIDLNDLKRCWNIAERTPWFSSFIPF
                     IRSKAQVEDKHEESLAERIRNKPEEVVLGESVNTDYKIATCCNPIAGDDVIGLITPGM
                     PMLIHRANCKRAQDQMSRFGNRIVKAKWKQHDPLLFLAGIKFTSIDKTGLIYQLADII
                     SNKYNLKIRSFNLTSSNEVSEGKIMLYVKDIANLNELISEIRTIKEIEKVSRMNPE"
     gene            7814..8305
                     /locus_tag="PK345_05000"
     CDS             7814..8305
                     /locus_tag="PK345_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPX34407.1"
                     /translation="MYSEAKDNSFPFESVKKIFVDYLEQKKHRKTPERFTILKEIYNT
                     PGHFDVETLYIRMKNNKYRVSRATLYNTIELLLDCNLLIKHNFGNNCAQFERSLRFDK
                     HDHFICTQTGDVKEFYDPRIIEIKNTVEKELGVKITHHSLTFYGECNGGSLKNVIDVD
                     SNN"
     gene            8473..9759
                     /locus_tag="PK345_05005"
     CDS             8473..9759
                     /locus_tag="PK345_05005"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HPX34408.1"
                     /translation="MKVDVLLGLQWGDEGKGKIVDVLTPEYDVIARFQGGPNAGHTIK
                     FEGLKFVLHTIPSGIFNPNKTNVIGNGVILDPVIFRKEINQLIKTGVNPKDNLLISRK
                     AHLILPGHRLLDAVYEKAKGRTMIGSTLKGIGPTYTDKVARQGLRIGDIEKQFFLEKY
                     NHLKELQQRIVNTYKVNISNFEIDGLSFNEYEKLWFDSLEILKQFQAIDSEYFINQAI
                     DNNKKVLAEGAQGTMLDIDFGSYPFVTSSNTVSAGVCSGLGVAPSKIGKVFGVFKAYC
                     TRVGNGPFPTELNDETGQMIRETGNEYGATTGRPRRCGWLDLTALKYSIMLNGVTDLI
                     MTKGDVLTGFEKIKVSNGYLSGNQKINHVPYDIEPVLPIYEEIDGWQSPVETIATYDE
                     LPDALKDYVNIIEKRTKTPISIISVGPDRSQTIIRS"
     gene            complement(9762..10337)
                     /gene="nadD"
                     /locus_tag="PK345_05010"
     CDS             complement(9762..10337)
                     /gene="nadD"
                     /locus_tag="PK345_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005865778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000309 - nicotinamide-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_function: GO:0004515 - nicotinate-nucleotide
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate (nicotinamide) nucleotide
                     adenylyltransferase"
                     /protein_id="HPX34409.1"
                     /translation="MIKTGLFFGSFNPIHIGHLIIASYIHQFTDLEEVWIVVSPKNPL
                     KPELELLEEEERLKMAQLAVEHNPSLKVCDIEFYLPKPSYTIDTILRLESDHPGRQFV
                     IIAGTDIFKDFHRWKEWENLISGYQFYIYNRPEYDAGDFANHPSVKIIKAPLLDISST
                     FIRKALSNGYDIRYMLTEKVWDYIKSKKLYH"
     gene            complement(10344..10913)
                     /gene="gmk"
                     /locus_tag="PK345_05015"
     CDS             complement(10344..10913)
                     /gene="gmk"
                     /locus_tag="PK345_05015"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006281316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HPX34410.1"
                     /translation="MKHGKMVVVSAPSGAGKTTIVRRLLASGLNLEFSISACSRQPRA
                     NEKNGVDYYFISEGEFRKRIKRSEFVEWEEVYDGCFYGTLKQEVERIWAAGNHVIFDV
                     DVKGGLNIKKNYTDICLSLFIQPPSLAELERRLRGRITDSEERIQQRLKKSVYELTFA
                     KHFDRIIINDNLDQAVQEAHNAIATFIEK"
     gene            complement(10913..11791)
                     /locus_tag="PK345_05020"
     CDS             complement(10913..11791)
                     /locus_tag="PK345_05020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HPX34411.1"
                     /translation="MIRSMTGYGKAEESISGRKYIAEVKTINSKQFDPLVRLPTNLKH
                     RELDIRSLLAAMLERGKIDFTLSIEGSDRQEEMVLNKPLLKKYYQELVSLENELEIKR
                     ENPFGLDSLLQLPNVFELQNDISDEEIWDDLLEIVQKAIADCDTMRIAEGTNLKSDFI
                     ARTDMILDLIEQIEPFENERILKIREKLQKEINENVAADKIDQNRMEQEIIFYIEKMD
                     ITEEKVRLKNHCDYFLQTLKEDGPVGKKLGFIVQEIGREINTLGAKANDWNIQRIVVL
                     MKDELEKIKEQLFNIL"
     gene            12125..12198
                     /locus_tag="PK345_05025"
     tRNA            12125..12198
                     /locus_tag="PK345_05025"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12159..12161,aa:Ile,seq:cat)
     gene            12345..12998
                     /locus_tag="PK345_05030"
     CDS             12345..12998
                     /locus_tag="PK345_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HPX34412.1"
                     /translation="MKSNIKIPDNLILDFGGVIYQIDHLRQIEAFVNLGIKDFGALFS
                     KAHQSDIFEDFECGKLTAGEFIKAIKELLGNVEVSKERIIKAWNSILIGFMDDAVSLL
                     KKLKDRTNLYLLSNTNEIHYEAFMAEFNRKYNFDFNSLFIRTFWSYQVGMRKPDNAIY
                     NHLVQQCNLNPEKTLFIDDSLQNVEGAINAGLPAYYLKPPERLSDIAINNWQLAINN"
     gene            13338..13751
                     /locus_tag="PK345_05035"
     CDS             13338..13751
                     /locus_tag="PK345_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008617736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HPX34413.1"
                     /translation="MVHQCEINLPVFKRGFHLITHLIEKQIIGLVQTGFVQVFVKHTS
                     AAITINENADPTVLTDMEEYFDRLAPEDQDYFEHTLEGPDDMPAHIKTTLIGHSVSIP
                     ITNGRLNLGTWQGIYLCEFRSRAQARKLIITVVGE"
     gene            complement(13752..14357)
                     /locus_tag="PK345_05040"
     CDS             complement(13752..14357)
                     /locus_tag="PK345_05040"
                     /inference="COORDINATES: protein motif:HMM:NF016239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008770 - [acyl-carrier-protein]
                     phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
                     /protein_id="HPX34414.1"
                     /translation="MNYLAHLYLSFGHDDVLVGNFIADRVKGKQLYDYPLGIQKGIIM
                     HRKIDAFTDRHDVVKRSKSRIRDRCRKFSGIVTDIYYDHFLSSLWLQYSDEPLLQFTR
                     NCYKTLFRYYFIMPSRLKRILPWMAAGNWLYSYRDIDYISIVLKRFASRINFDSGIEN
                     GATELRLHYRELQQDFQEFFPELIEYSKGLMEHPPHLAPVR"
     gene            complement(14357..15481)
                     /gene="meaB"
                     /locus_tag="PK345_05045"
     CDS             complement(14357..15481)
                     /gene="meaB"
                     /locus_tag="PK345_05045"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009432448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HPX34415.1"
                     /translation="MELSEYIESEGSALKINVGVNQPEQINPAALNRLRKHRHITFSA
                     DEYVQAILSGDRMMLSRAITMIESINPTHQAIAQEIIEKCLPHSGKSLRVGITGVPGA
                     GKSTFIEALGMYVTSLKHNLAVLAIDPSSSRSKGSILGDKTRMEELSTNEKAFIRPSP
                     SGGSLGGVARKTKETIILCEAAGFDVIFVETVGVGQSETAVHSMVDFFLLLMLAGAGD
                     ELQGIKRGIMEMADAIFINKADGDNRQKANRSRLEYKNALHLFPPTESTWKPVTGICS
                     ALTKEGIPEVWDTIKKYQDLTIRNGYLKKKREEQKSISMLETISESLNNDFYATEKIK
                     SLLPAIRQEMLDDKISAYVAAQKLIDIYFDEKRKPTSPSL"
BASE COUNT         4856 a         2891 c         3105 g         4808 t
ORIGIN      
        1 gatctcaaaa aaagaaaatc taccgccttt tgtcatcttc caggatccct cgctcgaaga
       61 tatggctata cagtatccta ttacgatgga tgaactaacg aagattacag gcgttggcgt
      121 gggaaaggca cagaaatatg gaaagccatt tcttgatttt ataaaaaaat atgtagatga
      181 aaatgagatt gtccgcccta atgatatggt ggttaagtcg gtaattaata aatcagggtt
      241 gaaggtttat atcattaaaa gtattgatcg aaaactgccg cttgaagaca tcgcatttac
      301 caaagattta agttttgacg agctattgac cgagttggag agcgtagtgg catcgggaac
      361 taaaattgat atcaattatt acatcaacga agtagtagac ccctatcacc aggaagagat
      421 aatcgaatat tttcaggaag ccgaatccga ctctgttgaa aaagctttgt tagagcttgg
      481 agaggacgaa tttaatgaat atgaaatcag actgatgcgc ataaagttta tgtctgatgt
      541 agggaactga tgattttaat atttgatcaa aacatggcgt tcaacttatt gaatgctgtg
      601 tttttgttca aggcaaatcc aaaagcatga ccaacaagct ttttgttttc tttttaactc
      661 ttgctttagt ggcaggatgt ggccctgttg aagagaataa atcaaaaaaa atacctcaaa
      721 atattcttaa tctagataag atggcggagc ttatcgccga tcttcaaatt gccgagagcg
      781 tcctcaatga gctgcagcgt aacggagagt atgacaaaag taaagccgca acactgatga
      841 gcagcatatt tgaaactcac ggtataacac aacaagcatt tgatgagagc cggctgtttt
      901 atgagaataa ccctcatctc tatgaattgg tttacgaaaa agtgatcaca ctcctaacag
      961 aaaaacagac ggaattgacg gtgaaggcac agaaagattc cgttttaaga aaaatgccgc
     1021 tgccacaata agctattcct tttttgggat cagcttaaat cgccaaaaat tgtggcttcg
     1081 aagatataaa tttcggcctc cctccatccg tcccatccga ttttagcttt gtctctggca
     1141 caatgaccaa gaaactcttc cactgtccag tggtaattat cagcaacctg cggaaggaaa
     1201 actccgttgt tagttccttt ttttataaaa atcccatgct tgcccaattg aatctctttt
     1261 gaagatttaa ttttttgcaa aggagttaac accgaaattt cgacagatag taaatcaagc
     1321 tcttcggctg ttatttcatc aaaacgacta tcttcagtgg ctgctgatac cgccaattgt
     1381 ttcactgcct gccacaatgg ttctgtattg ttaaaccttc ccaaacagcc ccgcaactga
     1441 ccgttgacat aaacggaaac aaaaactcca ttttgtgaat ttaatgcttc ggatttaaaa
     1501 tcgggtgttg tcccctcagt tgctacacct aagatagcct ccttgattga atttttcgcc
     1561 attgaaataa gcatctcctt ctcttgctcg gtaaaagttg caggcttaga cttttgataa
     1621 aaagctattg cccaatagcc aacaacacct tttttgtcgc catacaaagc atcacccgaa
     1681 tttttatatt ctatcgggat aatttcaatg tttttgtctt tgctgctgat attgagtaaa
     1741 acaagcattg aactccagcc gcacattgca gttgccaggc catgtattgc tgattgttca
     1801 acttttttta ttgcagaaag aaacgttttc ggagagtttg tggaaatggc ctcggcaatt
     1861 atctgatcgg cacgttttgc atcatcatac tttggataat gagaaaaatc gctactgata
     1921 acaaaaagat tatcagggtt aaaaaaaggt tctaaagcgc tagatatctg ttctaataca
     1981 tgaagacttt gagtggagat aattattgga actattttga atttcttttg taaatgatat
     2041 tgaagaaaag gcaattgtac ttcaagactg tgctctttga ggtcggcggc agggttataa
     2101 gtaaagcgct catgctcaga aatcagttta ttagccagat caatatctac ctctacttta
     2161 ccaagcggcg tcaggtagtc gccctgatta tataccgatg cgccgtcgaa agaaacccgg
     2221 tggcttgatc caatcaagaa aattctatca aactctgaat gtgggtcaat ctggttaaaa
     2281 gctgatgctg ccacagcgcc ggagtaaata tatccggcat gaggagcgag gattgcaatt
     2341 ggagaaccga ctcttggagg agcagctaca ttgaaatagc tcttcaaatc agcttttaac
     2401 tcttttgctg aagcaggata aaattgccct gccacacaag gctgcctgtc caacgatttg
     2461 tgaaatctgt cggagggttt gtcttttttg aaaaataagt tgccaggcat tgattgttga
     2521 attataggat attaagtata aaaatggggg tgagcaaatg tacaaaaaat ttaaatccgt
     2581 attaataaat taaaagccct gtgttaactc tttacgccat gggcattttt agaatcggct
     2641 tatcttagta gcgtaattga tcctttgatt gttctcttgg taagtccttc gagttggatt
     2701 tcaaacacat cgcaaacata aaaatatact ccctcgctac agtttctgtt gttttgttgg
     2761 tttttgccgt cccattgaaa ccatggatcg gtggtttggt aaacgattct tccccaacgg
     2821 ttgaaaatga tgagattaat tctttcgacc gaagtaaatt caaaggcttt aaacctatca
     2881 ttataagtgt cgtcgttggg agtaaataca ttgggtagtc ggtatgtgcc acattcgctg
     2941 atatcaacgc attggatatt actcatctga ctttgatttc ctgctgagtc cacggcaatt
     3001 actccataac atcctgcaat cgagaaaagg tttttatgag tatagttagt tgtgaatggg
     3061 tcatcaattg aatcgagtaa tactaattca ccctcttgtt gtggagaata aaatatcagg
     3121 tatttggcaa tatcagtcgg gcagccggat ggattatacc atgtcagtat attttccacc
     3181 gcatcgcagt tggtggttgt ttcaagcaat tgagcgcaag gcggatcaat atcaataggc
     3241 gtttccgaaa ctatttgcga gaaattgatg attggactaa caattgactg tattgagtaa
     3301 tagcctgtgc tacgaacaaa atacgtataa ttctctccat ttataagatc tgaatcgcga
     3361 tagttgttgt tagcgctaag gccaattgag tcgtattcaa gtgtttccgg attttgcctg
     3421 aatatcaaat agaagtcatt atcccacgga acgttgacat cccaattgag agtaagagtt
     3481 ctatcggaag gttctatttt tagaaatatt gattgcgccg tatgtgttga gccaatgaaa
     3541 actcgattgg taaagggttc actgtatagg tcaactttgt agagataact gtttttcgta
     3601 gtgtttaatt ctttgtcaat gaaaatggta tcgttgatgc tcaatgtcga atcgcaaacg
     3661 gaaaatgaat ttgggctatc ggttgtggct ctttgtataa tgtaatgata tggagcaggg
     3721 tataaaatgg tgtccaactc ggtaggtttc gaccatgcca caaatatttc acccgcactt
     3781 tgcgaggtta cttccacaga aacattggtg atgattggta tatctctttt caggctggca
     3841 caagcctctt gtgatgcgat gcttttaccg ccgtcagaaa aaagagtaac taccatatag
     3901 cagtaatcaa tgccatgtat tagacctgtg ccgttgttgg tgtcaataaa tgtagtgtcg
     3961 gatatgttaa gcgtttcgcc tattttttga tatccggtat agttggggac tccagtttca
     4021 caatgttgag gttgatagcc atagaggccg tttcgccggt atatctcata tttaagagct
     4081 tgtgtgcata atgctttttg ccagctaaga tgtattgagt taccaaccga tgaggcgctt
     4141 aaattttcag gccccgggct tattacttct atgtttactg ttttaatatc aactaattga
     4201 ggaaagttta acgagtcttt tgcggtaaac actatttgat agggttgtag ttgaacgtgt
     4261 gaacatgctg gagtccatgt aaaggtagat tgtacacttc cttttcccga tgctgaaggg
     4321 aaagatgccg gattttcagg taaaattagt gggccgccca acgcatggag cgaaagccag
     4381 tcattatcag gatcggtggc ggaaacggta aacgagagcg tctcgccaac aactacacaa
     4441 tcgtcctgta acggtgaaat aataggaggc tgattactac aagctgtgat tgcaatttgc
     4501 atgtcgcggg ttacataacc gatcctaacc ccatagcgcc attcttcaat aaggaaagca
     4561 atgttatact cgccttgcat cagaggctta tcccaaataa tggtgcctga aaaagggtct
     4621 atctcaaaag ttgtcggaat ttgtgtgttt acttggttgg ggaaaatata gccagggata
     4681 ggtaatccgc cttctccccg acatattgtt aacttgtaag aaaggctgtc gccgtcaata
     4741 tcataggctg ccggattatg catataaatg ctgtttacgc aacctttatc aataggagct
     4801 gccaaaagct gaacagaatt attagggcca agaaacggat tgatgactaa ctccgtttgt
     4861 aaaaaaaatg gtacattaac cgaattgggg atatttaaaa cgccataatt tcggttaggg
     4921 tcctctaaac ttattgtata tgatccttga ccgggaaatg tatgtactat gccatcatac
     4981 tcattgcggc taatgttgtt gggcagatca atctttttgg tacgttgcac tattgacgaa
     5041 gagccatctc cccaatttat aggtaattcg ggacggtcgg ccgggcttgg tgtgtaagta
     5101 taggtaacga ttttgaattc atatttcaga tcggaaattg cagtataggt gatttcccct
     5161 gcgcgttggt gcgtacctaa aacggcaagg ctcaaaaaga aaaatgacaa aaaaaataga
     5221 atctttcgca tctaaaaatc aaccgttagc aagatttcca aaagtaggat tttctgaata
     5281 ctatgataaa aataaaattt taaacccgct ccgaaaagtg atcttaacta caaatccatg
     5341 aatttttgaa aaactttgtt ctcagggcgg attcaattat taaatatcaa tatgtactct
     5401 tctaacgaaa aatactgttg ttttattcaa acgagattat gagttgtttg gtttatttaa
     5461 ttttgtaacc tcgattctaa gcagaaatct tctattagta tgtatagcga agaggaacat
     5521 aatgagataa gtaataaata caatgaactc attgggtgtt ggaaggctca acctgataaa
     5581 ggcgagttgg agatgataaa taaggccttc gatctcgctg tacatgttca tagtacgatg
     5641 cgacgccgtt ctaaagagcc ttatgtctat catcctattg aggtggcaat aatttgcgct
     5701 aaagatatga ggcttgatgc catctccatt gcctgcgctc ttcttcacga tgtagtggaa
     5761 gatactgagg ttaccataga agacattcag accattttca accaacaaat agcaacgatc
     5821 ataagtgggc ttacaaaaat taaagatgta ttagatgata gcacccgctc gatgcaggcc
     5881 gagtatttta aacagataat cctttccttg agtaacgata tgcgggtgat catgatcaaa
     5941 atggctgacc ggctacataa tatgcgaaca ttagaacaca tgagccgcga caaacaactg
     6001 aaaatagcct cagaaaccct tatgttgtac gctccttttg cttatcggat gggactttac
     6061 aatataaaaa cagaattgga agacctgtcg ctcaaatata ctgaacccga attttacaat
     6121 catatttaca agcagcttga aaataccgaa cctaatcgtt caaagttcat catgcagttt
     6181 gtaaagccca ttgaggagat attgagcaaa acagagataa aacatcagat tcaggtaaga
     6241 accaagtcgt tgtatggcat ttatcagaaa atgtcgcagc aaaatatctc ttttgatgag
     6301 gtgtatgata tttttgcaat taggataatt ctcgatgttg atatagaaca agagcgattg
     6361 gaatgttgga aagtctattc gctcattact gaaatttata aaccgaaaca cgacagactg
     6421 cgcgattgga tatccatccc taaagctaat gggtatgctg ctcttcatac taccgtgatg
     6481 agcaaaacag ggaaatgggt ggaggttcag ataaaatcaa accgaatgca tgagattgct
     6541 gaaacaggct tcgcctctca tcactattat aaaaacatta ctgatgaaaa caaagtaata
     6601 gaggagtggc tgaatagaat cagagatcaa tttcaaaatg cagataatga tgcgcttagt
     6661 tttcttgacg actttaaact tgatatatac tctgacgaga tttatacctt tacaccaaag
     6721 ggggacatga taaaattacc cgtcaactcc tcggcccttg attttgctta cgcaattcac
     6781 accgaaatag gcaatcggag cattggcgcc aaagtaaacc tgacattagt gccgctcaaa
     6841 catactttaa caagcgggga tcaggtggaa attattacat ctaaaaaaca aacccctttg
     6901 ccggaatggc tcaattatgt tataacaaca aaagcaaaat accatattaa agaggcgctt
     6961 aagattgaaa agaagaagca ttattcggat ggtaagcaaa agttgaatga gctgtttgaa
     7021 actgttggaa tagaatttag taaacagaat atcaagaagt taaaagactt tattaagatt
     7081 gaaagtctta tagaccttta ttatgaagct gacgtgggtc gaattgatct taacgatctg
     7141 aaaagatgtt ggaacattgc agagagaact ccatggttta gctcattcat accttttatt
     7201 agaagtaaag cccaagttga agataagcat gaagaatctt tggctgaacg aataagaaac
     7261 aagccggaag aggttgtgct tggcgagtcg gtaaataccg attataaaat tgctacatgc
     7321 tgtaatccta ttgcaggcga tgatgtaatt ggtttgatta caccagggat gcctatgctc
     7381 attcaccgag caaattgtaa aagagctcaa gaccagatgt ctcgcttcgg taaccggata
     7441 gttaaagcta aatggaaaca acatgacccg ctgctttttt tggccggtat aaaatttaca
     7501 agtattgata aaacaggact tatctatcaa ttggccgaca taatttccaa caagtacaac
     7561 ctgaaaatta gatcctttaa ccttacaagt tctaacgaag tatcagaggg caaaataatg
     7621 ttgtacgtca aagacattgc taatcttaat gaattgatca gcgaaataag gacaattaaa
     7681 gagattgaaa aggttagtcg aatgaatccg gaataaaaat cttgacgtaa caattaaaat
     7741 agtttgaatt aaaaaaataa tacttttgca gtctattttt attttgacta aataactaat
     7801 cggagatttt aatatgtata gtgaagctaa agataacagt tttccatttg aatcagtaaa
     7861 aaagattttt gttgattatc tcgaacaaaa aaaacatcgt aaaacaccgg agcgctttac
     7921 aattcttaaa gaaatatata atacgcccgg tcattttgat gttgagacct tgtacattcg
     7981 catgaagaat aataaatata gagttagccg cgctactttg tataatacta ttgaactttt
     8041 gctcgactgc aatttgttga taaaacataa ttttggaaat aactgcgctc agttcgaacg
     8101 ttcattgcga tttgataagc acgatcattt tatctgtacc caaacaggcg atgtaaaaga
     8161 gttttacgat ccacggatta tcgaaataaa aaataccgta gagaaggagt tgggcgtgaa
     8221 gattacacac cattcactta cattttatgg cgagtgtaat ggcggatcac ttaaaaatgt
     8281 gattgatgta gatagtaata attagaggct gatttagaat attactaaaa catattttat
     8341 cgccggaatc tgtaccgaca gtttccggct ttattgtgcc ctgtaatttt atggaaagat
     8401 tattttagct gcaaattgat tatttttaac cactcgatta attgatttta atagtattaa
     8461 taaacgaaaa agatgaaagt tgatgttctt ttaggtcttc agtggggcga tgagggtaaa
     8521 gggaaaatag ttgacgtgtt gacgcccgag tacgatgtta ttgcacggtt tcaaggagga
     8581 ccgaatgccg gtcatacaat caagttcgaa ggattgaaat ttgttttgca tacaatccct
     8641 tcaggaattt ttaatccgaa taaaactaat gtcattggca atggagtaat acttgatccc
     8701 gtaatttttc gaaaagagat caatcaatta ataaaaacag gtgtaaatcc taaagacaac
     8761 cttttaattt cccgaaaggc gcatttgata ttacctggcc acaggctgct cgatgcagtg
     8821 tacgaaaagg ctaaaggcag gactatgatt ggatccacgt taaaaggaat cggccctacc
     8881 tatactgata aagtggctcg acaaggtttg agaattggag atattgaaaa gcaatttttc
     8941 ttggaaaaat ataatcattt aaaagagcta cagcagcgta ttgtaaacac gtacaaagta
     9001 aatattagca actttgaaat tgatggacta agttttaatg aatatgaaaa actatggttt
     9061 gattcgcttg aaatattaaa acaattccaa gccattgata gtgaatattt cataaaccaa
     9121 gctattgata ataacaaaaa agtgttggca gagggcgctc aaggtactat gctcgatatt
     9181 gattttggat catatccctt tgttacctct tccaatacgg taagcgccgg cgtatgctca
     9241 gggcttggag tggctccatc aaaaataggt aaagtctttg gtgtgtttaa agcctattgc
     9301 acccgtgttg gtaatggacc ttttcccact gaattgaatg atgaaacagg acagatgatc
     9361 cgggaaacgg gaaacgaata tggcgcaaca accggacgcc caaggcgttg cggctggttg
     9421 gatttaaccg cactgaaata ttctataatg ctgaatggag ttaccgacct gattatgaca
     9481 aaaggagatg tgttgaccgg ttttgaaaaa ataaaggtta gcaacggtta tctatccggt
     9541 aaccaaaaaa ttaatcatgt tccttacgac atcgaacctg ttttacccat atatgaggag
     9601 attgacggct ggcaatctcc tgttgagaca attgccactt acgatgagtt gcccgatgct
     9661 ctgaaggatt atgttaatat cattgaaaaa cgtactaaaa cgccaatttc gataatatcg
     9721 gtaggacctg atagaagcca gactataatc cgctcttaaa cttagtgata taactttttt
     9781 gattttatat aatcccacac cttttcagta agcatatatc gaatgtcgta gccattggaa
     9841 agcgcttttc tgataaatgt agaggagata tctaataaag gagctttgat gatctttact
     9901 gaaggatggt ttgcaaagtc tccggcatca tattcagggc ggttgtaaat ataaaattga
     9961 tagcccgaaa taagattttc ccactctttc caacgatgaa agtccttaaa aatatccgtt
    10021 ccggctatta ttacaaactg cctgcccgga tggtcagatt ctaaccttaa tatggtatct
    10081 atagtgtagg atggtttggg caaatagaat tcaatatcgc aaactttcaa tgatggattg
    10141 tgttctactg caagttgtgc cattttaagg cgttcttctt cttcaagtag ttctaattcg
    10201 ggcttcaatg ggttctttgg cgaaactact atccaaacct cttccaaatc agtgaattgg
    10261 tgaatatagc ttgcaataat caaatgcccg atgtgtatgg ggttgaacga accaaagaaa
    10321 agcccggttt ttatcataat tgtctatttt tcaataaatg ttgcaattgc attatgagcc
    10381 tcttgaacag cctgatcgag gttgtcgttt attattatcc ggtcgaagtg tttggcaaag
    10441 gtgagttcat aaactgactt tttaagtctt tgttgaattc tctcttccga atcggtgata
    10501 cgcccgcgca aacgtctttc taattctgcc aacgatggcg gctgaataaa aagggaaaga
    10561 caaatgtcag tgtaattctt tttaatattg aggccgcctt ttacgtcaac atcgaaaata
    10621 acatgattgc cagcagccca tatcctttca acttcctgct ttagtgtgcc ataaaaacaa
    10681 ccatcataaa cctcttccca ttcgacaaac tccgatcttt ttatcctttt tctaaattcg
    10741 ccttcactta taaagtaata gtccacacca tttttttcgt ttgcccgcgg ttgccggcta
    10801 caggctgata tggaaaactc aaggtttagc cctgaggcca acagacgcct cacaatagta
    10861 gtcttacccg cacctgacgg agccgaaact acaaccattt tgccgtgctt catcataaaa
    10921 tattaaaaag ttgttcttta attttttcca attcatcttt cataagaact actatccgtt
    10981 gaatgttcca gtcgttggct ttagccccca aagtgttaat ctcacgtccg atctcctgaa
    11041 ctataaaacc taactttttc cctacagggc catcctcttt aagggtttga agaaaataat
    11101 cacaatgatt ttttaacctt accttctctt ccgttatatc cattttttcg atgtaaaaaa
    11161 taatctcctg ctccatacga ttttggtcaa tcttatccgc agcaacattt tcgtttatct
    11221 ctttttgtag tttttctctt attttgagaa tcctttcgtt ttcaaaaggc tcaatctgtt
    11281 caatcaaatc gagaatcata tcggtacgag cgataaagtc ggatttaaga tttgttcctt
    11341 cagcaatcct catggtatcg caatcagcaa ttgctttttg tacgatctct aaaaggtcat
    11401 cccaaatttc ttcatctgaa atatcgtttt gcaactcaaa cacattgggc aattgaagta
    11461 atgagtctaa gccaaatgga ttctctcttt taatttcaag ctcattttcg agcgacacta
    11521 actcttgata gtattttttt agcaacggtt tgtttaaaac catctcttcc tgccggtccg
    11581 aaccttcaat actcagggta aaatcaattt ttcctctctc taacattgca gccaagagtg
    11641 atctgatatc caactcccta tgcttcaggt tagttggcaa tcggaccagc ggatcgaatt
    11701 gtttgctgtt tatggtttta acctcagcaa tatactttcg gcctgagatt gactcttcgg
    11761 cttttccata gcctgtcatt gatcttatca tagttttttc agcaaattta aaataaaaaa
    11821 aacttgcaga ttatttattg ataattgatt atttgtacct atccaaaacc ataagttact
    11881 tgacaacgca accctcgaac tgctaagcct tttcgacaag attaatttca atattatttt
    11941 ttaaacacct gtttcgaaaa tgaataaaga ggtttttcgc aatcaatttt taaaaatctg
    12001 acaccacttg agtcattctt ttgtgaatag agtgataaaa tcttgaattt atttttttta
    12061 tttaatttgg aatatataat tttgcagccc tgaaatagtt agatgacttt tcatcttatt
    12121 cagagggcct atagctcagt tggttagagc aactgactca taatcagtag gtcgctggtt
    12181 caagtccagc tgggcccaca aataaaagga aaccggcttt tgataagatt gccggttttt
    12241 ttgtgatcaa aacagtacga aagtctttat taattgactt tgtaaaaaaa aaatataaat
    12301 atcagattat taataattta aatataaaaa agagagataa ctggatgaaa tcaaatatta
    12361 aaatacctga taatctgatc ctcgatttcg gtggagtaat ttatcaaatt gatcatttga
    12421 ggcagattga agcctttgtt aatcttggaa ttaaagattt cggcgccctc ttttctaaag
    12481 cacaccagtc ggatatcttt gaggattttg aatgtggaaa acttactgcc ggcgagttta
    12541 tcaaggctat taaagaatta cttggcaatg ttgaagtatc aaaggagcgt attataaaag
    12601 catggaatag catattgatt ggttttatgg atgatgcggt atcgttattg aaaaaattga
    12661 aagatcgtac caatctttac ctactcagca ataccaatga gatccactat gaagctttta
    12721 tggcagagtt caacagaaaa tataatttcg attttaactc tttgtttatc agaacctttt
    12781 ggtcatacca agtgggaatg agaaagcccg ataatgctat ctacaatcat cttgtgcaac
    12841 aatgtaattt aaatcctgaa aaaacactct ttattgatga tagtttgcaa aatgttgaag
    12901 gcgcaataaa tgcaggatta cctgcttact atttaaaacc gccggaaaga ctatctgata
    12961 ttgcaattaa caattggcaa ttagcaatta acaattagca attagcaatt aacaattggc
    13021 aattggcaat tggcaatttg gatgggttga ggggtgaagg gggttgtttt caagtcttaa
    13081 ttctctgttt tttacaaaat tttggcgccg aattgctttg taaagttgcg tagctaatat
    13141 taattgaaca gggcaaagta aaaatctatg gttaaacctt ggtgtgcaaa cgtttgggat
    13201 atagtagaat tggggagtag ctgaatatga tatactcaaa tttaaataca cgaatccttg
    13261 aaaaacttcg ttttcagagt gggtaaagcc tttattaccg atttttttgt ttaatatttg
    13321 aatgaaattt tgattttatg gtgcatcagt gcgaaataaa tctgcccgtt tttaaaagag
    13381 gctttcatct gataactcat cttatcgaga aacagataat tggattggta cagaccgggt
    13441 ttgtgcaagt ttttgttaaa catacctcgg cggctattac cataaatgaa aatgccgatc
    13501 ccactgtact tactgacatg gaggagtatt ttgacagatt agcgcccgaa gatcaagatt
    13561 attttgaaca tactcttgaa ggccctgacg acatgccggc acatattaaa acaacgctta
    13621 tcggtcattc ggtctccata cccataacta acggtcgcct taatctcggt acatggcaag
    13681 ggatatatct atgcgagttt cgtagccgcg ctcaagcccg taagctaata attactgttg
    13741 ttggagaata gctatcttac gggagccaaa tggggcggat gctccataag gcctttagaa
    13801 tattcgataa gctcagggaa gaactcttga aaatcttgct gtaactcacg ataatgaagc
    13861 cttagctcgg ttgctccatt ttcaattccc gaatcaaaat ttatccttga agcaaacctt
    13921 ttaagcacga tgctgatata atctatgtcg cgataggagt aaagccagtt gcctgcagcc
    13981 atccatggca aaatcctttt tagccgcgaa ggcatgataa aataataacg aaacagggtt
    14041 ttatagcagt tgcgcgtaaa ctgcaaaagc ggctcatcag aatattgtaa ccataatgaa
    14101 ctaaggaaat gatcgtaata gatatcggtt acaatcccgg aaaatttgcg acaacgatct
    14161 ctaattcttg atttgctacg ctttaccaca tcatgccggt cggtgaaagc atcaattttt
    14221 cgatgcatga ttattccctt ttgtattcct aaaggataat cgtacaactg cttgccttta
    14281 accctatcgg caataaaatt tccaactaat acatcgtcgt ggccaaagga taagtataaa
    14341 tgggcaagat aattcatcaa agagagggcg aggttggttt tcttttttca tcaaaataga
    14401 tgtcaatgag cttttgagca gcaacgtaag cgcttatctt atcgtcgagc atctcctgac
    14461 ggatagcggg caagaggctt tttatttttt cagtagcata aaagtcgttg ttaaggcttt
    14521 cgctgatggt ttctaacatg ctgatacttt tctgttcttc tcttttcttc tttaaatatc
    14581 cgtttcttat tgtcaaatcc tgatatttct ttattgtatc ccacacctcc ggtattccct
    14641 ctttagttaa tgccgagcat attccggtaa cgggtttcca tgttgattcg gtaggcggaa
    14701 aaaggtgcaa tgcgtttttg tattccaacc ttgaccggtt agctttctgg cggttgtcgc
    14761 cgtcggcttt gttgatgaaa atagcgtcgg ccatctccat tattccccgt tttatgcctt
    14821 gaagctcgtc gccggcgcca gccaacataa gtaaaagaaa aaaatcaacc atcgagtgaa
    14881 cagccgtttc cgattgaccg acacctaccg tttcgacaaa tattacatca aatcctgctg
    14941 cttcacacag gataattgtc tcttttgttt ttctggcaac tcctcctaat gagccgcccg
    15001 aaggcgaagg gcggataaat gctttttcgt ttgttgacag ctcctccatc cgtgttttat
    15061 cacctaaaat actgcctttt gaacgactgc tgctgggatc aatggctaac acagcaagat
    15121 tatgctttaa agaggtaaca tacatcccaa gggcttcaat aaatgtgctc ttgccagcgc
    15181 caggaacacc tgtgatacct accctaagcg atttccccga atgaggtaag cacttttcga
    15241 tgatctcctg agcaatagcc tgatgggtag gatttatact ttcaatcatg gtaatggctc
    15301 tgctgagcat catacgatcg cccgaaagaa ttgcctgtac atactcatca gcgctaaaag
    15361 ttatatgacg atgcttcctg agacgattta atgctgccgg attaatttgc tcaggctgat
    15421 tgacgccaac atttatcttt agcgccgagc cttctgactc tatatattca ctcaattcca
    15481 tcaatttctg attttatttt acaaaaatat attttatttc ctcaaccaat ggagtggcga
    15541 aatgaaattt gaagtgtaag aatttgtttg aggggctatt aaaaggatat ttgataaggt
    15601 tagcctccac taaaattgta agtagatttt ccccctcaat ggaaaaagtg aaggtaacgg
//
LOCUS       DAOOAI010000050        15572 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_4926_length_15572_cov_5.024425, whole genome shotgun sequence.
ACCESSION   DAOOAI010000050 DAOOAI010000000
VERSION     DAOOAI010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15572)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15572)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15572
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4926_length_15572_cov_5.024425"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..258)
                     /locus_tag="PK345_05050"
     CDS             complement(<1..258)
                     /locus_tag="PK345_05050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34416.1"
                     /translation="MRRSINILKINPKLVLIVCLIPVFFLSLSCKTYKTFKINVLEPA
                     EIIVPQNIKHILIAPNNKLHSNGLTYEFYDEKYIDTSFNQKE"
     gene            complement(277..1728)
                     /locus_tag="PK345_05055"
     CDS             complement(277..1728)
                     /locus_tag="PK345_05055"
                     /inference="COORDINATES: protein motif:HMM:NF041518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choice-of-anchor Q domain-containing protein"
                     /protein_id="HPX34417.1"
                     /translation="MKNTLLISGLVLMMIASCQKDEKINSSPSVKLFFSADTIIFDTV
                     FTTIGSITKEISVYNKESEKIVISSINLAGGNNSRYSINVDGVPGTHFNDVEIGPNDS
                     IFVFARVNINPGDADLPFIVTDFIVFETNGNIQDVDLVAWGQNANFILWDTDRPGLPK
                     YKIVAAEGVDTTWTKSLPIVVYGYAVVDSTGSLTIEPGTKVHFHSGSGLWIYKGGSLK
                     VLGTLEEPVYFQSDRLDGFYQYLPGQWDRIWINEGAVDNVIEYAVIRNGFIGLQLETL
                     QEYMGNQLFLNNTIIENMSGAGILTRFYLVASHNCVFTNCGQYALALTLGGSYDFRHV
                     TIGNFWNYTVRTTPSIYVNNYYLDQNNQAVEFPFNGNFANSIIYGNQREEIVVDDMGN
                     DFTLKFDHCLLKTEKDINESEIFINSLKNQDPLFVDYTVNDLQLDSISPARDIGKIEI
                     ANTVPTDLKGVDRLESPDIGAYEWIPVIETKKR"
     gene            complement(1933..3174)
                     /locus_tag="PK345_05060"
     CDS             complement(1933..3174)
                     /locus_tag="PK345_05060"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007753341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPX34418.1"
                     /translation="MKFDDLNLYPDLLQGINSVGFETPTPIQQSTIPLILDGYDLIAC
                     AQTGTGKTAAYVLPILHHILTSDLNENVIKALIIAPTRELALQIDQQLEGFSYFAGIS
                     SIAVYGGGSGSDFELEKKAIKTGTGVVVATPGRLISHLNLGYVNLKNLKFFVLDEADR
                     MLDMGFLPAIQQIVSYLPSKRQTLMFSATMPKEIRTLAKKIMHNPKEITLELAKPPEK
                     VLQAAYFVRKELKTPLLKHLIQDKDLPSILIFVATKNGVKELEKELANSKFDVKAIHS
                     DLDQQQRNETLRLFRNKEVQILVSTDIVSRGIDIEDISLVINYNVPQNAEDYVHRVGR
                     TARAEKTGMAITFVNNAEIKSFKRIEQLIAKEIFIAPLPDVLNEKNKERNRGEKLKKA
                     KLSIPKRGSYYKRKSRKSDKK"
     gene            complement(3258..4094)
                     /gene="pyrF"
                     /locus_tag="PK345_05065"
     CDS             complement(3258..4094)
                     /gene="pyrF"
                     /locus_tag="PK345_05065"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HPX34419.1"
                     /translation="MTRNTLIKIIQEKKSFLCIGLDTDLAKIPSFLHGYHDPVFEFNK
                     AIIDHTQDLAVAFKINTAFYENRGIKGWTSLEKTVRYLDQYRSSIFLIADAKRGDIGN
                     TSQMYAGAFLADPPHGLGFDSITVAPYMGNDSVKPFFYHKGKWVILLALTSNAGFEDF
                     QTKRLESGEMLFEYVIRQSMQWGNIDNLMFVIGATRQDMLSKVRQIVPNHFLLVPGVG
                     AQGGSLKAVAQTGLTKDFGLLVNSSRGILYAVDDESFPQAARTEALKIQQEMAIYLQH
                     LL"
     gene            4471..6009
                     /locus_tag="PK345_05070"
     CDS             4471..6009
                     /locus_tag="PK345_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HPX34420.1"
                     /translation="MLVKTYGSAIQGVNAITITVEVNIDQGVNFFLVGLPDSAVKESQ
                     QRIEAALRYNNYRLPGKKIVINMAPADIRKEGSAYDLTIAIGILAASEQMAADTVGDY
                     IIMGELSLDGGLQPIKGALPIAIEARKKGFKGFILPSQNAREAAIVNDLEVYGIENIR
                     EVIDFFDGKAKLTPTLINTREQFYAGLNQYEFDFSDVKGQENIKRALEIAAAGGHNAI
                     LIGPPGSGKTMLAKRLPSILPPLNLHEALETTKIHSVAGKMSRDSSLISIRPFRSPHH
                     TISDVALVGGGGNPQPGEISLAHNGVLFLDELPEFKRTVLEVLRQPIEDRVITISRAK
                     FTVDYPASFMLIAAMNPCPCGYYNHPDHECVCGPGVVQKYLNKISGPLLDRIDIHVEV
                     VPVPFRELSEARSAEPSADVRQRVIKARQIQEKRYSKNKGIHCNAQITSKMLREICKI
                     DSVGQELLKNAMEKLNLSARAYDRILKVSRTIADLDGSEKIKPEHLAEAIQYRSLDRE
                     SWGN"
     gene            6035..6505
                     /locus_tag="PK345_05075"
     CDS             6035..6505
                     /locus_tag="PK345_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34421.1"
                     /translation="MRRLMIAFLLASISLITFGHEITPETFRNHALQIAKHPKITKKF
                     SMIDMEENFHLYMDVTSFDEFYVKEPIETQGFKLFLWKSGEMGFYNIEQYVTLITVLQ
                     PSADKVIYELVSQVDHKRFVIQVLFENQQDGWQLTAVKRTKIKTAKPSIFPKFK"
     gene            6619..7353
                     /locus_tag="PK345_05080"
     CDS             6619..7353
                     /locus_tag="PK345_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013064549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX34422.1"
                     /translation="MQRQQIIQLIDIKKYYKVGTHIVKALQSISLEIYKNEYVALMGA
                     SGSGKSTLMNIIGCLDTPTSGQYFLNQKDVSKLDDNQLAEIRNKEIGFVFQTFNLLSR
                     QTALENVMLPLVYAGMNKAKRIKRANEVLENVQLLDRIAHKPNELSGGQRQRVAIARA
                     LVNEPSIILADEPTGNLDSKTSLEIMGLFEQIHKLGNTIIIVTHEEDIALHAHRIIKL
                     KDGLVEDDYMNKTIKTMTDYTMESVN"
     gene            7394..7954
                     /locus_tag="PK345_05085"
     CDS             7394..7954
                     /locus_tag="PK345_05085"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPX34423.1"
                     /translation="MPAKHKIYTKKGDKGETSLIGGMRVPKYHQQIETYGTIDELSSF
                     VGLIRDQDIDEGTKATLIKIQNRLFTAATQLAVAPDYIPQRQLPAIKEEDIEFLENQI
                     DKMNDSLPPITGFILQGGHQTVSFCNIARAVCRRAERVTISLSKNQPVNDLIIKYLNR
                     LSDYLFVLSRKLTKDLKAVETAWKTI"
     gene            8033..8254
                     /locus_tag="PK345_05090"
     CDS             8033..8254
                     /locus_tag="PK345_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002996718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2795 domain-containing protein"
                     /protein_id="HPX34424.1"
                     /translation="MYWTLELASKLEDAPWPATKDELIDYATRSGAPPEVIENLNEIE
                     DEGEIYERIEDIWPDYPTKEDFFFNEDEY"
     gene            complement(8251..8793)
                     /locus_tag="PK345_05095"
     CDS             complement(8251..8793)
                     /locus_tag="PK345_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmD family RNA methyltransferase"
                     /protein_id="HPX34425.1"
                     /translation="MRIIGGTQKGRHINPPANLPVRPTTDLAKEGLFNILNNIIDFEG
                     LKVLDLFAGTGSISFEFASRQAAAVVAVDLNFKCVEFIKKTSLQLGFLHLKAIRTDVF
                     RFLSKSPNEGFDLIFSDAPYDIDNVMILPNLVFNHNWLNEDGLLIIEHTRSLQFAGHK
                     HFSQTRRYGKVNFSFFTAKG"
     gene            complement(8781..9653)
                     /locus_tag="PK345_05100"
     CDS             complement(8781..9653)
                     /locus_tag="PK345_05100"
                     /inference="COORDINATES: protein motif:HMM:NF024271.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3822 family protein"
                     /protein_id="HPX34426.1"
                     /translation="MSARIKLSKSIFDKALNKEATNYLGLSIQLSTDGFSFCILNPKE
                     AKYLALQSYKFKDSSNQMALSQLLDELIPSVDWLNLPYEGVKILMETHKSTLVPHAFY
                     DSEYSKEQFAFNATINKDDILLNDYLPLLDAINVWLINGKLYDTIIKYFPLAVIHNHG
                     SVLIESALALANSHREETPVFTHVRKNWFDVVVINNGKLVFYNSFRYQAKEDFIYFLI
                     YVLEQLELNPDVIQLYLLGEVLKISEIYDITMMYIRNVSFALPFDNVSFGFNLGDIPL
                     HFYFNLLNHYQCEL"
     gene            complement(9669..10244)
                     /locus_tag="PK345_05105"
     CDS             complement(9669..10244)
                     /locus_tag="PK345_05105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34427.1"
                     /translation="MKKRIIQIVLLIVIVFLGYRVWETIQTPVRFLKEKDHRQALVVQ
                     KLKDIRSVQQMYRTLNDSFAPNLDLLVHFLKTAEIPVVNVIYDPTDTLLVKTISDTLG
                     FKPVLDSLFDTRTDFKLDEFDIIPFSDGVKFEMDAKMINKGGVMVPAFMAVAKKEYYL
                     NGLDENMIKNPLVKDMIVGSLEEPTTDGNWE"
     gene            complement(10298..13498)
                     /gene="infB"
                     /locus_tag="PK345_05110"
     CDS             complement(10298..13498)
                     /gene="infB"
                     /locus_tag="PK345_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005813807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HPX34428.1"
                     /translation="MKESGTIRLNKAAREFNVGQGTIVEFLLKKGFQIDQNPNTRLTG
                     EMYELLSREYIGEKSVKEEAMKKDISSVKREVLTIDDRKTISSESRYEDDDEDLIIRG
                     VKIEIDEEFKKAIESPAIRKTETKDLDELVEDLLIEKPVENDVLPDRVIDIDSEQEEP
                     EKEIIAAPDKIKPKRTKKTTPKAETEKAEHKEEVIDYQKPVEEQQPAEAKKIQDTKII
                     AEEEKKTEAKKESDDKEKDIGLKIIGKIDLDSINERTKPPKKTIEEKRKERESKKKTE
                     VKKPKTTIKKEKIETTVKEKPKKVVKGKDDTVVKKEAKQKDVQIETPPPLPPTKPIEK
                     EVEFIKLKVERLTGPNILGKIELPETKRPEKQKPIASSSDEKVKTKRKKRKRIKPEEA
                     KAEPVKPKPAEKQPEEKAKTKAKIRLQRHKRLKKDDRPVLTDEDIQKQIKETMARLTS
                     GGKSKASKHRRQKRDLISKIKEKELQEIEEEKSIIKVAEFVTANEMASMMNVPVTQII
                     SLCMQLGLVVSINQRLDAETIIIVAEEFGFKVEFTSVESAIDEADFEEDRPEDLTQRS
                     PIVTVMGHVDHGKTKLLDYIRRTNVIAGEAGGITQHIGAYEVNLENGKRITFLDTPGH
                     EAFTAMRARGAKVTDIAIIVIAADDNVMPQTKEAINHALAAQVPIVFAINKIDKPTAK
                     PEKIKEQLSALNILVEDWGGKYQSQEISAVTGQNVDVLLEKVLLEAELLDLKANPNRR
                     ALGTVIESSLDKGRGFVAKLLVQNGTLKVGDIVLAGLHSGKVKAMYNARNKRVNQAGP
                     SSPLLVLGINGAPQAGDIFRVMSDEREARNIAAKRTQLQREQGLRTQKHITLDEIGRR
                     IAIGDFKELNLIVKGDVDGTVEALSDSLLKLSKPEVQVNVIHKSVGQVTETDVMLASA
                     SNAIIIAFQVRPSVGARKLAEHEQIDIRLYSIIYTAIEEIKSAIEGMLSPEIEEKITC
                     NVEVREVFKIAKVGTIAGCMVLDGTITRNTMVRLIRDGIVVYTGKLASLKRFKDDVKE
                     VNSGYECGLSVENFNDIKIGDIIEGYEMIEIARKFD"
     gene            complement(13548..14783)
                     /gene="nusA"
                     /locus_tag="PK345_05115"
     CDS             complement(13548..14783)
                     /gene="nusA"
                     /locus_tag="PK345_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HPX34429.1"
                     /translation="MDNLNLIETFSEFKDFKNIDRETMMRIIEDVFRSMLEKRFGSSD
                     NIDIIVNIDKGDLEIYRLREIVEDDQVEDPNTQIAYSDAIKIEPDFELGEEVSEEIRM
                     SEFGRRDILAIKNNLETKVRDYEKNSIYNKYKDRIGEIVTGEVHQINAREILVFDDEG
                     MELILPKSEQIPNDHYRKDDTIRAVVLKVDIKGNAPMVILSRTASVFLERLFELEVPE
                     VYDGLITIQNIVRVPGERAKIAVESYDDRIDPVGACVGMKGSRIHGIVRELRNENIDV
                     INYTSNISLYIQRALSPAKITSIVLDEKNKRAEVYMKPDQVSLAIGKGGFNIKLAGKL
                     TGYEIDVYRDTDIDAEDVDLDEFNDEIEQWIIDAIKAAGFDTAKSVLNQEFDELVKLT
                     DLEEETISDVIKILKAEFE"
     gene            complement(14815..15267)
                     /gene="rimP"
                     /locus_tag="PK345_05120"
     CDS             complement(14815..15267)
                     /gene="rimP"
                     /locus_tag="PK345_05120"
                     /inference="COORDINATES: protein motif:HMM:NF002531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0042274 - ribosomal small subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly cofactor RimP"
                     /protein_id="HPX34430.1"
                     /translation="MIKKSYIKELADRHLQGTDKFVAEVVVKSGNVITIVIDSDTAVL
                     IDDCINLSKAVESSLDRDVEDFELRVTSYGADKPLISKRQYVKNIGRDLQVVTNENLK
                     LIGKLINVDKENITLELPINKKKKESVPEILNVPFAEINQTKMVLAFK"
BASE COUNT         4651 a         3035 c         2774 g         5112 t
ORIGIN      
        1 ctctttttga ttgaaggaag tatcaatata tttttcatca taaaattcgt aagtaaggcc
       61 attgctgtga agcttattat taggtgcaat taaaatatgt ttaatatttt gtgggacaat
      121 aatttcggct ggttccagca cattgatttt aaaagtcttg taagttttgc aagaaaggct
      181 gaggaaaaaa actggaatca ggcaaacaat taaaactaat tttggattga tttttagtat
      241 gttaatacta cgtctcatca ttaattaaag ttagttttac ctcttttttg tctcaattac
      301 cggaatccat tcatacgctc caatatcagg gctttccaag cggtcaactc cctttaaatc
      361 ggtagggacg gtattggcga tttctatctt accgatatca cgagcaggcg aaatgctatc
      421 gagttgaagg tcatttacgg tgtaatcaac aaacaaagga tcctgatttt ttaatgaatt
      481 gataaatatt tcagattcat taatatcttt ctcggttttg agcagacagt ggtcgaattt
      541 cagtgtaaaa tcatttccca tatcatcaac tacaatctct tctcgttgat ttccataaat
      601 tatggaatta gcaaaatttc cattaaatgg gaactcgacc gcctgattat tttgatctaa
      661 ataatagtta ttgacataaa ttgaaggcgt tgtacgaacg gtataattcc agaaattacc
      721 tattgtaaca tgtcggaaat catagctgcc accaagagtg agcgctaaag catactgacc
      781 gcaattggtg aaaacacagt tgtgggatgc cacaaggtaa aatcttgtaa ggatcccggc
      841 gcctgacata ttctcgatga tggtattatt caaaaagagc tgattaccca tatattcctg
      901 taatgtttct agttgaagtc caataaagcc atttcttatt acggcatatt caattacatt
      961 gtcaactgca ccttcattta tccaaattct atcccattgc cctggcaggt attgataaaa
     1021 accatccaat cggtcgcttt gaaaatagac aggttcttct aatgtaccta atacttttaa
     1081 cgagccgcct ttataaatcc aaagtcctga tccggaatga aaatggactt ttgttcctgg
     1141 ttcaattgta agggaacctg ttgaatcaac aactgcatag ccatatacaa ctatcggcag
     1201 agatttcgtc cacgttgtat caactccttc agcagctaca atcttatatt tgggcagccc
     1261 tggccggtct gtatcccaaa gtataaaatt tgcattttgc ccccatgcta caaggtcaac
     1321 atcctgaata ttaccatttg tttcaaaaac aataaaatct gttacaataa atggcagatc
     1381 agcatcaccg ggattaatgt tcactctggc aaaaacaaaa atactgtcat taggcccaat
     1441 ttcgacatcg ttgaagtgcg tacccggtac cccgtcaaca ttaatagaat atctcgaatt
     1501 gttacctccg gcaagattga tagaagagat gacaattttt tccgactcct tattgtaaac
     1561 gcttatttcc ttggttatcg aaccgatagt ggtaaaaaca gtatcaaaaa taatggtgtc
     1621 ggcgctaaaa aagagtttta ccgaagggct actatttatt ttttcatctt tctggcaaga
     1681 tgctatcatc ataagaacca aaccagatat cagaagtgta tttttcaaac gacttatggc
     1741 catttattca agagggaggg tatataacaa actaaattta ctcaccaagt tacgtttttt
     1801 ttttcggttt attgcaaata acgcatgatt taatatctat actttatcac tgttttgata
     1861 aataatcatg atataaatgt gttaagtaca aatcaatgat cattgtaatt tttttaaaat
     1921 tcgttctgaa aactactttt tatcggattt gcgtgatttt cttttatagt agcttcctct
     1981 ctttggtatt gataatttcg ctttttttaa tttttcacct ctatttcttt ctttattttt
     2041 ctcattaaga acatcgggca agggcgctat aaaaatttct ttagctatca attgctcaat
     2101 tcgtttaaag cttttaatct cagcattatt aacaaaagtg atagccattc cggttttttc
     2161 tgcacgagcc gttcttccta ctctgtgtac gtagtcctca gcattttgag gcacattata
     2221 attaataaca aggctaatat cttcgatatc aatcccgcgc gatacaatat ctgttgacac
     2281 aagtatttgt acctctttat tacggaaaag acgcaaagtt tcattacgtt gctgttgatc
     2341 caggtcggaa tgtatggcct ttacatcaaa cttgctattt gcaagctctt tttccaactc
     2401 ttttactccg tttttagttg cgacaaaaat caaaatagat ggcagatctt tatcttgaat
     2461 caaatgttta agcagtggtg ttttcaattc ttttctaaca aaataggctg cttgtaaaac
     2521 tttttcgggt ggtttggcaa gctctaatgt gatctctttt ggattgtgca ttatcttttt
     2581 ggccagagtc ctaatttctt tgggcattgt tgccgaaaac attaacgttt gacgttttga
     2641 tggcagataa cttacaattt gttgaatagc aggtaaaaaa cccatatcca acatccggtc
     2701 agcttcgtcg agtacaaaaa attttaggtt tttcaaattg acataaccta agttaagatg
     2761 agaaataagc ctgccagggg ttgcaactac gacacccgtt ccggttttta tagctttttt
     2821 ctcaagttca aaatcactac cggaaccgcc accatagaca gctattgagc tgatgccggc
     2881 gaaataagaa aaaccttcta actgttggtc aatctgcaaa gctaattcgc gggttggtgc
     2941 aattataagg gctttgataa cattctcatt taaatcactg gttagaatgt gatgtaatat
     3001 aggaaggaca taagccgcag ttttacctgt tcctgtttga gcacaggcaa tgagatcgta
     3061 accatcgagt attaaaggta ttgttgattg ttgaataggc gtaggggttt caaaacctac
     3121 ggaattaatg ccttgtaata gatcaggata taaatttaaa tcgtcaaact tcaaaattgt
     3181 aggaatgaaa aaaattattt acaaaactat agcttatcaa agcaatatca acaagaactg
     3241 cggcaaaggg ttatgtgtta tagcaaatgt tgaagataaa tggccatttc ctgttgaatt
     3301 ttcaaagcct ccgttcgcgc agcctgagga aaactttcgt catctaccgc atacaatatc
     3361 ccccttgatg aattaactaa taatccaaaa tcttttgtaa gtccggtttg ggctactgct
     3421 ttcaagctgc cgccctgagc gcctacacct ggtacaagca agaaatgatt tggaacaatt
     3481 tgcctgacct tagataacat atcctgacgt gttgccccta tcacaaacat cagattatca
     3541 atatttcccc attgcataga ttgccggata acgtattcaa aaagcatctc tccgctctcc
     3601 aatctctttg tttggaaatc ttcaaaaccc gcatttgagg ttaatgccaa tagaataacc
     3661 catttgcctt tatgataaaa aaaaggtttt accgaatcgt tacccatata cggcgcaaca
     3721 gtaattgaat cgaagccaag accgtgggga ggatcggcca aaaatgctcc agcatacatt
     3781 tgcgatgtgt taccgatatc acctcgtttg gcatcagcaa ttaaaaagat ggaactccga
     3841 tactgatcta aatagcgaac agttttctca aggcttgtcc atccttttat acctctgttt
     3901 tcataaaatg cggtatttat cttaaaggca acagccaaat cttgagtatg atcaataata
     3961 gctttattaa actcaaaaac cgggtcgtgg taaccatgga gaaaagatgg aatcttggcc
     4021 aaatcggtat caagtccgat acaaagaaat gatttctttt cctgaataat tttaatcaga
     4081 gtatttcttg tcattgtttt gcataattga gaggctaaaa gtaaacatta atcttgtaaa
     4141 tggagaaaaa ctactgatgt caaaagttta catcgtaatt attaaatgtt tagaccgaaa
     4201 aaaatgcgaa tggctctcag tttcatttaa ttcatttgat aagattaaga tatagtacct
     4261 tgtttaaaaa aaagatactc cttggaatta actccacata aaatcgctaa taaaataaca
     4321 atctatgttt atccattgct ttagaaactt gctttcaaca ttttcttttt tttaacaagt
     4381 aaaaactcta ttttctatta aagtgttgcc tgattcggat cattttgttt attttgcaaa
     4441 acatcaattt catatctttt agtctcattt atgttagtaa aaacatacgg tagcgctatt
     4501 cagggggtta atgcaatcac catcactgtg gaggtcaata ttgaccaagg ggtgaatttt
     4561 ttccttgtag gcctgccgga tagcgctgtg aaggagagcc aacaaaggat cgaagcggca
     4621 ttacgttata ataattatag gttgccaggg aaaaagatag tcatcaatat ggcgcctgct
     4681 gacataagaa aagagggttc tgcttacgat ctgacaatcg ctattgggat tttagctgca
     4741 agtgagcaaa tggcggctga cacagtcggc gattacatca tcatgggtga gctatcgtta
     4801 gacggcgggt tacaaccaat taaaggggct ttgcccattg ccatagaggc ccgtaaaaaa
     4861 ggctttaaag gttttatcct tccatcacaa aatgcacgcg aagccgctat tgtcaacgac
     4921 cttgaagtat atggaattga aaatatccga gaggtgatag atttttttga cggtaaagct
     4981 aaacttacgc caacattgat caatacacgt gaacagtttt acgcaggctt aaatcagtac
     5041 gagtttgatt tttcggatgt taaggggcag gaaaatatta aaagagcctt ggagatagct
     5101 gctgccggcg ggcataacgc tattcttatt gggcctcctg gctctggtaa aacgatgctt
     5161 gccaaacgat tgccatcaat cctaccgcct ttaaacctgc acgaagcatt agaaactaca
     5221 aagatccatt ccgttgcagg aaaaatgagt cgtgattcat ctttaatctc catccgcccc
     5281 tttcgttcgc ctcaccatac tataagtgat gtagcactgg ttggcggcgg cggaaaccca
     5341 cagccaggcg aaatttcgtt agcacacaat ggagtccttt ttctcgacga attaccggag
     5401 tttaaaagaa cagtacttga agtattgaga caacctattg aggatagagt gataactatt
     5461 tcaagggcga aatttactgt tgattatcct gccagcttca tgcttattgc cgcaatgaat
     5521 ccttgcccat gcggatatta taaccatccc gatcatgaat gtgtatgcgg accgggagtt
     5581 gtgcagaagt atctgaacaa gatttcaggg ccactgttag atcgtattga tattcatgtg
     5641 gaggtagtgc ctgtgccttt cagagaactt tcggaagcga gaagtgcaga gccaagcgct
     5701 gatgtacggc aaagggtaat aaaagcacgg caaattcaag aaaaaagata ttcaaaaaat
     5761 aaaggaatcc actgcaatgc gcaaataacc agtaagatgc ttagggaaat ttgcaaaatt
     5821 gacagtgtag ggcaagaatt gctgaaaaat gcaatggaaa agctgaattt atcagctcgg
     5881 gcatacgaca ggattttaaa agtatcgaga acaattgctg accttgatgg ttcggaaaag
     5941 ataaaacccg aacatctcgc cgaagccata cagtaccgaa gcctcgaccg tgaaagctgg
     6001 ggaaactgat taaaaaatta atatttagtt gaatatgaga cgattaatga ttgcgttttt
     6061 attagcctct attagcttaa ttacttttgg gcacgaaatt acacctgaga catttcgaaa
     6121 tcatgctctc caaattgcca aacatcctaa aataactaaa aaattctcga tgattgacat
     6181 ggaggagaac tttcatctct acatggatgt tacatcattc gatgaatttt acgtaaaaga
     6241 gcctattgaa acacagggtt ttaagctttt tttatggaaa tcgggtgaaa tgggatttta
     6301 caacatagag caatatgtta cactgattac tgttttacag ccttcggccg ataaggtaat
     6361 ttacgaactc gtatcacaag tagatcacaa gaggtttgtt atacaggttt tatttgaaaa
     6421 tcagcaagat ggctggcaat taacggcggt gaaacgtact aagataaaaa ctgcaaagcc
     6481 aagtatattt cctaagttta agtaggaaaa tatttatttg atcaaagatt gtaatatttt
     6541 tataatcttc gacaatattc gtatttcttt tctgttctga aatttcgtgt ttgttcacat
     6601 taaaacgtaa gtatcgagat gcaacgacag caaataatcc aactgattga tattaaaaag
     6661 tattacaagg ttggaacaca tatagttaag gcgcttcaat caatctcttt agaaatctat
     6721 aaaaatgaat acgttgcttt gatgggcgct tccggttcgg gtaaatccac attgatgaat
     6781 attattggat gccttgatac tccaacgagt ggtcagtatt ttcttaacca aaaagatgtg
     6841 agtaagttgg acgataatca gcttgctgaa attaggaata aagagatagg atttgtattt
     6901 cagactttca atttattatc acgtcagaca gctttagaaa atgtaatgct tcctttagtt
     6961 tatgccggaa tgaataaggc aaagagaata aaacgagcca acgaagtgct tgaaaatgtg
     7021 cagctccttg atcgaattgc ccataaacct aatgagctat ccggaggtca gaggcaaagg
     7081 gtagctatcg ccagagcgtt agtaaatgaa ccttcgatta tacttgccga tgagccgaca
     7141 gggaacctcg actctaaaac ttcattagaa ataatgggac ttttcgagca gattcacaag
     7201 cttggaaaca caataataat tgttactcac gaagaagata tcgccctgca tgcccaccgg
     7261 ataattaaac taaaagatgg acttgttgaa gatgattata tgaataaaac cattaaaacc
     7321 atgactgact acactatgga atcggttaat taaaataaat tagccatttt taaccttaaa
     7381 tttgaatctg aaaatgccag caaaacataa aatttatact aaaaagggtg ataaaggtga
     7441 gacttcgctt attggaggga tgcgtgttcc taaatatcat cagcaaatag agacgtatgg
     7501 tacgattgac gaattaagct cgtttgttgg gctaatacgg gatcaggata ttgatgaagg
     7561 aactaaagca acacttatca aaattcagaa tcggcttttt accgctgcta cacaacttgc
     7621 tgttgctccc gattatattc ctcaacgaca actccctgca attaaagagg aggatatcga
     7681 gtttttggaa aatcaaatag ataaaatgaa tgatagctta ccccctatca ccggttttat
     7741 tttacaaggc ggtcatcaaa cggtatcatt ttgtaatata gcgcgtgccg tatgtaggcg
     7801 agccgaacga gttacaataa gtctgagcaa aaatcaacct gtaaatgatc ttatcataaa
     7861 atatttgaac cgcctatcgg attatttgtt tgtactttcg cggaagctta caaaagatct
     7921 aaaagctgtg gaaacggcat ggaaaacaat ttaataacaa aatttattta tatagttaca
     7981 atctatttat cgcaaaaaaa attagttaaa cattaaaaat gaaaatcgag ctatgtattg
     8041 gacattggaa ttagcttcta agcttgaaga tgcgccttgg ccggcaacta aagacgagct
     8101 tattgattac gcaacgagat cgggcgcacc gccagaggtt atcgaaaacc ttaatgaaat
     8161 tgaagacgaa ggcgagattt atgaacggat tgaggatatc tggcccgatt atcctactaa
     8221 ggaggatttc ttttttaatg aagatgaata ttaaccctta gcagtgaaaa acgaaaaatt
     8281 taccttgccg tagcgtctgg tctggctaaa atgtttatgg cctgcgaatt gcaaagagcg
     8341 tgtatgctca atgataagca ggccatcttc atttaaccag ttatgattga agaccaaatt
     8401 aggcaagatc attacattat caatatcgta aggggcgtca gaaaaaatta aatcaaagcc
     8461 ttcgtttggg cttttgctca gaaatctgaa aacatctgtc cgaattgctt taagatgcaa
     8521 gaagccaagt tgtaaagatg ttttttttat aaactcaacg cacttaaagt ttaaatctac
     8581 agcaacaacc gctgctgcct gacgcgacgc aaactcgaag gatatacttc ctgtccctgc
     8641 aaagagatct agcactttta atccttcaaa atcaatgatg ttgtttagga tattaaataa
     8701 tccctcttta gccaagtcgg tagttggtcg tacaggaaga ttggcaggcg ggttgatgtg
     8761 tcttcctttt tgtgtgccgc ctataattcg cactgataat ggtttaaaag attgaaataa
     8821 aaatgcaacg gtatatcacc caaattaaag ccaaaactaa cattgtcaaa aggtagggca
     8881 aaacttacgt ttctgatata catcatagtt atatcgtaaa tctccgatat ctttaaaacc
     8941 tctcctaaca gataaagctg aattacatcg gggttgagtt caagttgttc aagtacataa
     9001 atcaggaagt aaataaagtc ctctttagcc tgatatctga aagaattgta aaagactaat
     9061 ttgccattat tgatcactac aacgtcgaac cagttttttc ttacatgggt aaatactgga
     9121 gtttcttcgc gatggctgtt tgccagagca agtgcactct ctattaacac gcttccatga
     9181 ttatgaatta ctgccaaagg gaaatattta ataatagtat cataaagttt accattgatt
     9241 aaccacacat ttattgcgtc gagcagaggc aaataatcat ttaacaggat gtcgtcctta
     9301 ttaatagtag cgttgaaagc aaattgctct ttactatatt cggaatcata aaaggcatga
     9361 ggaactaatg ttgatttatg tgtttccatc aatatcttaa cgccttcgta aggcaaattc
     9421 agccaatcaa cagatggtat aagctcatcc agaagctgtg agagagccat ctggtttgaa
     9481 gaatctttaa atttatagga ttgaagcgcg aggtatttcg cctcttttgg attgaggata
     9541 caaaaggaaa atccatccgt tgacaattgg atggataatc ccaaataatt tgtagcttct
     9601 ttgtttaaag ccttatcaaa tatgcttttg gataatttta tcctggcaga cattggcgaa
     9661 acatattgct actcccaatt accatcggta gtaggctcct ccagtgaacc gacaatcata
     9721 tctttcacta aagggttttt tatcatattt tcgtcaagtc cgttaaggta atattctttc
     9781 tttgcaactg ccataaatgc cggcaccatt actccacctt tattgatcat tttggcatcc
     9841 atttcaaact ttaccccatc cgaaaatggg atgatgtcaa attcatcaag cttaaagtca
     9901 gtccttgtat caaaaagcga gtcaagcaca ggtttaaaac cgagggtgtc gctgattgtt
     9961 ttcaccagga gagtatcagt tggatcataa ataacattta caacaggtat ctcggcagtt
    10021 ttaagaaaat gcaccaaaag gtctaagttt ggagcaaaag agtcgtttaa cgtacgatac
    10081 atttgttgta ctgatctgat gtctttcaat ttttgaacca cgagtgcttg acgatggtct
    10141 ttttctttaa gaaatctaac tggggtttga atggtttccc acacgcggta acctaaaaaa
    10201 acgattacaa taagaagaac aatttgaata attctttttt tcatagcgcc ttaattttta
    10261 agcaacaaaa atatattttt tttgaatatt aatgctttta atcaaatttt ctggcaattt
    10321 caatcatttc ataaccttcg attatgtcgc cgatttttat atcgttaaaa ttctcaacac
    10381 ttaagccaca ttcataccca ctattaacct ctttaacatc atctttaaag cgtttgagcg
    10441 atgccaattt tcccgtatat actacaattc catcacgaat taagcggacc attgtatttc
    10501 gcgttattgt accatcaagt accatacaac ctgcaattgt gccaaccttt gcaatcttaa
    10561 agacctccct tacttcaaca ttacaagtta tcttttcctc tatttcgggt gaaagcattc
    10621 cttcgatagc cgacttaatc tcttcaatag cagtatatat gatagaatag agtcttatat
    10681 caatttgttc atgttctgca agttttcttg ctcccactga cggcctaacc tgaaacgcta
    10741 taattattgc attggatgca gatgcaagca tcacatccgt ttcggtaacc tgaccaacag
    10801 atttatgaat aacatttact tgaacttcag gtttgctaag tttgagtagt gaatcgctca
    10861 aagcctcaac cgttccatcc acatcacctt taacaataag atttaattct ttaaagtcgc
    10921 caatagcgat tctacgtcct atttcatcaa gtgtaatgtg tttttgtgtt ctcaatcctt
    10981 gctcacgttg cagttgagtt cgtttggcag caatgtttct ggcctctcgc tcgtcggaca
    11041 taactctaaa gatgtctcct gcctgtggcg ctccatttat tccaagaaca agtaaaggtg
    11101 atgatggccc cgcttgatta acacgcttat tacgtgcatt atacatcgct ttaacttttc
    11161 ccgaatgtaa tccggctaaa acgatatcgc ctacttttaa agtgccattt tgaactaaaa
    11221 gctttgccac aaaacctcta cctttatcta atgaagattc aataactgtt cccaaagctc
    11281 tacggtttgg gttagctttt aaatcgagga gttctgcttc aagtaagact ttttccagca
    11341 aaacgtcaac attttggccg gtaactgctg aaatctcttg actttgatat ttcccccccc
    11401 aatcttcaac gagaatattc agcgctgaca gttgctcttt aattttttct ggttttgctg
    11461 ttggtttatc aattttgttt atagcaaaaa caattggcac ttgagctgcc agagcatggt
    11521 ttatggcctc tttggtctgg ggcatcacat tatcgtcggc tgctataacg atgatggcga
    11581 tatcagtaac ttttgctcct cgtgcacgca ttgcagtaaa tgcttcatgg ccaggtgtgt
    11641 cgaggaatgt tattcttttt ccattttcaa ggtttacttc gtaagcgccg atatgctgcg
    11701 ttataccacc agcttcacct gcaataacat ttgtccggcg tatataatcg agaagtttgg
    11761 tctttccatg gtccacgtgt cccattacag taactatcgg ggagcgttga gttaagtctt
    11821 ccggacgatc ctcttcgaaa tcagcttcgt caatagcgct ttctacactt gtaaattcaa
    11881 ctttaaaacc aaactcttcg gcaacaatga ttatagtctc tgcgtctaat ctttggttta
    11941 tagataccac caacccaagt tgcatacaga gggagataat ttgtgttacc ggtacattca
    12001 tcatgctggc catttcgttt gcagtaacaa actcggctac cttgataatg ctcttttctt
    12061 cttctatctc ctgaagctct ttttctttta tcttacttat caagtcgcgt ttttgtcgtc
    12121 tatgcttaga agctttggat ttgccgccgc tggtaagtct tgccatggtt tccttgatct
    12181 gcttctggat atcctcgtca gtaagaacag gacgatcatc ttttttaaga cgtttgtgcc
    12241 tttgaagtct aatttttgcc tttgtcttag ctttttcttc cggttgtttt tctgccggtt
    12301 ttggtttaac aggttcggct ttggcttcct caggttttat ccgtttacgc tttttcctct
    12361 ttgtttttac cttttcatcg gatgaggagg ctataggttt ttgcttttct ggacgctttg
    12421 tttccggcag ttcaattttt ccaagaatat ttggtccggt aagtcgctct actttaagct
    12481 ttataaattc tacttctttt tctatcggtt ttgttggcgg taaaggtggc ggcgtttcaa
    12541 tttgtacatc cttttgtttt gcctcctttt taacaacggt atcgtctttc ccttttacaa
    12601 cctttttagg tttctctttt acggtcgttt caattttctc ttttttaatg gtagttttag
    12661 gttttttaac ctctgtcttt ttcttacttt cccgctcttt acgtttctcc tcaatcgttt
    12721 ttttaggagg ctttgtacgt tcatttatag aatcaaggtc aattttacca atgatcttca
    12781 atccaatatc tttttcctta tcatcggatt ctttttttgc ttcagttttc ttttcttctt
    12841 cagcaattat cttggtatcc tgtattttct tagcctccgc cggttgttgt tcttcaacag
    12901 gtttctgata atctataacc tcttctttgt gctcagcctt ttcagtctct gcttttggag
    12961 tagttttttt ggttcttttt ggcttaattt tatcaggtgc agcaattatt tccttttcag
    13021 gctcttcctg ttcggaatca atgtctatca ccctgtcggg aagaacatca ttttcaaccg
    13081 gtttttcaat caaaaggtct tcgacaagtt cgtctaaatc tttagtctcg gtttttctaa
    13141 ttgcaggcga ttctattgcc tttttaaatt cttcatcaat ttctatcttt acgcccctaa
    13201 taatgagatc ttcgtcatcg tcctcatagc gcgattctga agatatcgtt ttacggtcat
    13261 caattgtgag cacttcgcgt tttacagaag aaatatcttt tttcattgcc tcttctttaa
    13321 cgcttttctc gccaatgtac tcacgtgaaa gaagttcgta catctctccg gtaagtcttg
    13381 tgttagggtt ttggtcaatc tggaagcctt ttttcaaaag gaactccacg atagtgccct
    13441 gccccacgtt aaactcacga gccgctttgt taagccttat tgtaccactt tctttcatat
    13501 tattcctatt acggtagaaa tcaaatattg cgctaaaaat acttttttta ttcaaattca
    13561 gcttttaaaa tcttgattac gtcgctaatt gtctcttctt ccaaatcggt aagtttgaca
    13621 agctcgtcaa attcttgatt taaaacactt tttgccgtgt cgaatcctgc tgcttttatt
    13681 gcatcaataa tccattgttc tatctcatcg ttgaactcgt caaggtcaac atcttcagca
    13741 tctatatctg tatctctgta aacatcaatt tcatatcctg taagcttacc ggcaagttta
    13801 atgttaaatc cacctttacc aatcgccagc gaaacctgat cgggtttcat atagacttct
    13861 gctcttttat ttttttcgtc taaaacaatg gatgtgatct ttgcagggct gagtgcacgt
    13921 tgtatataca aagagatgtt ggaagtatag tttatcacat caatattttc gtttctcaat
    13981 tctctgacga taccatgtat tcgggagcct ttcattccaa cacatgctcc aacagggtca
    14041 attcgatcat catacgattc tacggcaatt ttagctcttt cgccgggaac gcgaactatg
    14101 ttttgtatag ttatcaaacc atcataaact tcgggaactt ccagctcgaa aagacgctca
    14161 agaaaaactg aggcagtacg cgataagata accataggtg cattaccttt tatgtcaact
    14221 ttaagaacaa cagcccttat cgtgtcgtct ttgcggtaat gatcatttgg aatttgttcc
    14281 gattttggaa ggataagttc catgccttca tcatcaaata ctagtatctc tctagcgtta
    14341 atttgatgta cctcgccagt aacaatttca cctattcggt ctttatattt attataaata
    14401 ctgttttttt cgtaatcccg cactttcgtt tccaaattgt tcttaatagc aaggatatca
    14461 cgccggccaa attcactcat tctgatctcc tccgacacct cctctccaag ttcaaaatcg
    14521 ggttcgatct ttatagcatc cgaataggct atctgcgtat taggatcttc aacctgatca
    14581 tcttcaacga tttcacggag gcgataaatc tcaagatcac ctttatcaat atttacaata
    14641 atgtcaatat tatcagacga tccgaatctc ttctccaaca ttgaacgaaa aacatcttca
    14701 ataatacgca tcatcgtttc acgatcaatg tttttgaaat ctttgaattc cgaaaaagtt
    14761 tcgatcaaat ttaaattatc catttttagt ctatgtattt gtttcgagct ttttttactt
    14821 aaatgccaat accatcttcg tctgattgat ttcagcaaaa gggacattca aaatttcagg
    14881 aacactctct ttcttctttt tgttgatcgg aagttcaaga gttatgttct ccttgtcaac
    14941 atttatcaat tttcctatta gcttcaggtt ctcattagtt acaacttgta aatctctgcc
    15001 tatatttttg acatactgcc gttttgagat caagggtttg tctgctccat acgaggttac
    15061 cctaagctca aaatcctcca catccctatc gagcgatgac tccactgcct tactcaggtt
    15121 gatgcagtcg tcaatcagca ctgccgtatc actatcaata acgattgtga tcacatttcc
    15181 cgatttcaca accacttcgg ccacaaactt atcagtaccc tgaaggtgtc tgtcagcaag
    15241 ttcttttata tatgattttt taatcataat ttttactaat aaaagagggg acaacgtccc
    15301 ctcattccgt tcacataact gccattagta attgaaaaaa attttcaatt gcaaaaataa
    15361 tatttttaaa caaatcaata tgattaactt aatttttaca aaaagaacaa caaattataa
    15421 tcagttaaat aaatcgggaa acacggttat tcgagaaaca ctatgatttg cttttttagt
    15481 agtaaatagg tcatcttttg taaaaaaaaa actaattatt tttgtttgac tcaacaaggt
    15541 taaaaatgga aaaagtgatt gcatttctta gc
//
LOCUS       DAOOAI010000051        15457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_4976_length_15457_cov_5.746332, whole genome shotgun sequence.
ACCESSION   DAOOAI010000051 DAOOAI010000000
VERSION     DAOOAI010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 15457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15457
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4976_length_15457_cov_5.746332"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="PK345_05125"
     CDS             <1..748
                     /locus_tag="PK345_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34431.1"
                     /translation="YTLTTLFPGIGYQAFFINPLTINFPEYEGFLINQKNSFTNCKTN
                     PPWNIHPTDHYHLLSIQSESLYKIPNGFLGIFDEKNNCVGFSEITDDKSDNLLLIAYG
                     DDEWTLEKDGALEGEPMTFKLYRPSNQTEFMLNVTYSDNAPNHNGLFESFGMSIISDI
                     TMCGAEDVSHEINKIVIYPNPSQGIFTVSSEQLLGDVNWVVTDAKGQIITNGKLSDSQ
                     QINLCAQIKGIYFIRLTGEDIIHTEKLVIW"
     gene            complement(881..1624)
                     /locus_tag="PK345_05130"
     CDS             complement(881..1624)
                     /locus_tag="PK345_05130"
                     /inference="COORDINATES: protein motif:HMM:NF001030.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HPX34432.1"
                     /translation="MSGHNKWSTIKRKKGAMDAKRSKIFSKIIKEITIAVKESGPDPE
                     SNPRLRMAITNAKGASMPKDNIERAINKGKDKDASSFTEVAYEGYLPNGIAVVVECQT
                     DNLQRTISNIRSIFTRYGGTLGTSGSLSFLFERNGVFVLPKKEGLILDDFELEMIDAG
                     AEEIEIEEDTITVSTMMENFGNMQKKLEELDAEVEIAELQRVPNDTVTLDLESAKKIM
                     RAIEAFEDDDDVQKVFHNLELTEDIMAEL"
     gene            complement(1700..3760)
                     /gene="ppk1"
                     /locus_tag="PK345_05135"
     CDS             complement(1700..3760)
                     /gene="ppk1"
                     /locus_tag="PK345_05135"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:NF003917.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HPX34433.1"
                     /translation="MTKGEKKYPMINREISWLHFNDRVLQEARDPSTPLIERLRFLGI
                     FSNNRDEFFRVRVATLNRMLKVEQLDYRIKFSPKQVLNQIYTTVEAQEKVFTQTYVEI
                     VERLKEKNILIIDEQQLIPTQSKFVTDYFLNNIRQFLFPIMMENLKTTDDIEDRALYL
                     AVYMTSSVNPKKEDFAVIKLPTDQVSRFLILPEIDDKIYIMLLDDVVRHGLQYIFSIF
                     GYDTYHSYAFKITRDSELDFDSDVQKSFMEIMAESIKQREQGEPVRFVYDNSIPLPFL
                     KELRQKLNIQEDDHQRGGGRYHNFRDFMSFPIVGSPDLLYPPTEPLPHKGLTIHSSIF
                     REIRKRDIMLHYPYQSFQYIVDLLREASIDPAVRSIKMTFYRAASNSNVMNALINAAR
                     NGKPVTVFLEIQARFDEEANIYWVERLQKTGVKILPTIPGLKVHAKLILIRRQENQSN
                     VYYANISTGNFNESTAKVYADKSLLTADQDIATDVNKVFQLLESRYFIPKFKKLIVSP
                     FQTRDFFLKLINREIKNAKEGKEAWMVLKLNSLVDKKLTYKLYEASNAGVKIKIIARA
                     ICVLIPGVKGMSENIEAFGIVDKFLEHARVFVFCNNGDREFFISSADWMQRNLDYRIE
                     VTTPITDPQIQDDIWKILQIQLQDNTKARLISAENINRYRKTDSEIAVRSQFEIYNYC
                     KKNC"
     gene            complement(3766..4266)
                     /locus_tag="PK345_05140"
     CDS             complement(3766..4266)
                     /locus_tag="PK345_05140"
                     /inference="COORDINATES: protein motif:HMM:NF012521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HPX34434.1"
                     /translation="MKTIYILRHAKSSWDFSHLNDVERPLIEKGINRTNLIARYLNKN
                     NITPDLIISSAAVRALETARIVANAVKYPINKIHISKSLYSADEDNIITQLFDISDHL
                     HTVMLVGHNPTLTYFVNHYLEQKIEWLPTSGLVSISFDTDSWPEISNAGYVVNFLITP
                     SMLKKE"
     gene            complement(4434..5621)
                     /locus_tag="PK345_05145"
     CDS             complement(4434..5621)
                     /locus_tag="PK345_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013613426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HPX34435.1"
                     /translation="MSRQEFFIEINCQQRNHHGERICGDVFMQKRVKEENRIVVVLSD
                     GMGHGVKANMLATLTATMAMNFTAEHKDFEKIAEIIMKTLPVCSVRKISYATFTIVDI
                     EVGGVINILEYDNPETFIVRNNQVFDPGWQYMFLSSEKGEDKELRSCSFVAEKEDRII
                     FCSDGIVQSGLGTDSFPFGWEREQLIDFTLKEIKGNSLISAQELGEHIVKKAYQNDSY
                     ELKDDASCAVIYFRDPRKLLICTGPPYEEVNDKVYAKKVADFVGKKVLCGATTADIIA
                     REWEKTIVDSFDFIDPELPPISYMDGVDLITEGILTLTKVLNLLKDFNKNTMLDKGPA
                     GLIVNMILQSDEIDFIVGTRINIAHQDPSLPVDLEIRRTVVKRIAAILEEKFLKEVKV
                     EFI"
     gene            complement(5644..7497)
                     /locus_tag="PK345_05150"
     CDS             complement(5644..7497)
                     /locus_tag="PK345_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPX34436.1"
                     /translation="MALISIDPKNCTNCLACVRACPVHAIQTSVGNDIPTVVDARCIG
                     CGSCLGACRYDAISYWDSTGEVKELMKSEENVAAIVDPSISGEFPDITDYRKFVEMIR
                     SLGFKYVNEVSFGVDLVAKAYKMLFDEKKGKYYITANCPAIVYYIEKYFPSLIGNIAP
                     IITPVMATANVVRETYGEDVRIVYIGPCIAAKREILRSDGVTKIDSVLTFRELRKLFS
                     EKNIHESHLEFSDFNPPIGKLGSLYPASEGILEAGGINHAITDGDVIIAEGAETSLNA
                     VLSFDQNIDQIRKNFNVFYDEGCVMGPGMATTADKQLRIALVTEYAKKRLKDFDVEKW
                     EVEMAKHAKMNMKRVFKDDDQRLQEPDQAKLEEIMNLLGQADDGFTGCGGCGFKTCKD
                     FARAIAQGLTKTDICYDFSLRSKNKYINTLRESNKKSQEEVNAYKEELKKIKSEYDLA
                     TDKLETSREIMNQIPSGVVIVDERLKVTSSNRSFIELLGDDIKEIDDIIPGLRGADLK
                     TLVPVQFYKLFQNTLETGENIISRDVEIEESLLNVSVFSIKKNKVVGAIVRDMFSPEV
                     RNEQIIQRITEVVDQNLGMVQQIGYLLGEGASKTEAMLNSIIQLHKTKKQK"
     gene            complement(7509..7763)
                     /locus_tag="PK345_05155"
     CDS             complement(7509..7763)
                     /locus_tag="PK345_05155"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HPX34437.1"
                     /translation="MALVTLQKEVIICLGSSCFARGNKQMVQLIKKFLEEHNLSGKVN
                     FKGKHCFGNCENGPSIMIDQKRYEHINAESILEILENELL"
     gene            7904..8359
                     /locus_tag="PK345_05160"
     CDS             7904..8359
                     /locus_tag="PK345_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HPX34438.1"
                     /translation="MTEIKTVLQDYQNREREYLIPILQDIQSLIGFISPDAIAAVALH
                     LKIPTSKVYAVASFYDQFKFLPFAKYRIKVCNGTGCHIEGSGAIYDELVKLLGGETRT
                     ELFSIERVQCLGACGNAPVIKINDKFYIAVTLEMAADIIKSIKALEDAP"
     gene            8356..10308
                     /locus_tag="PK345_05165"
     CDS             8356..10308
                     /locus_tag="PK345_05165"
                     /inference="COORDINATES: protein motif:HMM:NF013662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HPX34439.1"
                     /translation="MIEKGQDTGLTGYLIEKVLKNYSGDYPPDVKREIDLLLRRHLRR
                     PVIYVGTGTCGIAAGADATLKAAKEYLKINQLEADLIEVGCVGLCSAEPLLDYQAPGR
                     ARVSFQHVTADKVYEILEECFHNHISDEHVLGQVASEGHELWHRIPLIQENPFFKKQH
                     RILLYDCGEISPTNLDEYIARDGYKAFLKVLMNYPPEEVCNIVGQSGLKGRGGGGFPT
                     ATKWNNARLMASDKKFVICNADESDPGAYMDRALVESNPYRLIEGISIAAYAVGASKA
                     YIYIRSEYNLAVKRLQNSLIRAKEAGFLGENIFNSGFNLQIILRQGPGAFVCGEETAL
                     IKSIEGHRGTPETKPPFPFEKGLFGKPTVVNNVETLINVPLILLHGPLWFKSIGTETS
                     TGTKLFALTGDTVNTGLVEVPFGITLEEIIYEIGGGIKNGASLKAIQIGGPSGSLVPA
                     AHSDLQIDYEAFHKRHQIIGSGGMVVMDENTCILDMIKYFINFLQKESCGKCIPCREG
                     TNRMYEILQMITTRPKQNDKHESLTRFQGVIQLENLAKVIKETSLCGLGKTAPNPVLS
                     ALEWFREEFEEHVFERKCRANVCKNLIEYVISAERCTGCMVCMKKCPYDAITGAPLEP
                     HLIIRDKCTSCGICYEVCKFSAIDFI"
     gene            10352..12073
                     /locus_tag="PK345_05170"
     CDS             10352..12073
                     /locus_tag="PK345_05170"
                     /inference="COORDINATES: protein motif:HMM:TIGR02512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016695 - oxidoreductase activity, acting
                     on hydrogen as donor [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase, group A"
                     /protein_id="HPX34440.1"
                     /translation="MIFNIEVNNKVLKAKRGDSILETLNQNGISVPTLCRMQGFPSSG
                     ACRLCVVEVEGKEDLVPACSHNVEEWMKIHTHSPRVINARRTIVELLLSSHPDDCLYC
                     IRNKNCELQELADELGIRERKLPIVKKQVKKDPSSVSITREPDKCILCGRCIKVCRDW
                     IGISTLDYIGKAMNTIVGPTFDNKLNISNCITCGQCIMVCPTGALHEKDDVNKVLLAL
                     QDPDKYVVVQISPTVSVSLAEEFNIRFGKELDELLVATLRRMGFNKVFKTAFAADIML
                     IQMAEELIDRMEKNERLPILSSNCPAFVKYIEQTYPDLIPNLSVIKSPQQIMGSLVKH
                     HFAKDQKVKAEKIFSVSLMPCTAAKFEASREEFTHKGNSDIDVTLTTRELARMIRLYG
                     LNIQQLAPEVSDTPFVIRTSASKIIGTSGGLAEALYRTLYYKLTGKQLINFKIAALRG
                     NKKIKEYNSKIGNHKIGIAAVNGLSNAVELLDKIIEGKSAYQFIEIMACPGSCVNGGG
                     QPIGSGQEAIKARIKTLYDIDEKESVKASYKNMAVTELFDKYIDKEKESFFQQFITSY
                     QKRDVML"
     gene            12155..14143
                     /locus_tag="PK345_05175"
     CDS             12155..14143
                     /locus_tag="PK345_05175"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPX34441.1"
                     /translation="MSHSKRKQLVFTVKDLCRVCYTCVRECPVKAIKIINGQAEVINE
                     RCIGCGNCFMVCSQGAKCFLKTIKDAKAIIAENEKVIAIVAPSYPAEFTEFPDHRQFV
                     GMIRALGFSHVCEVGFGADMVAREYRKLHDITLTGGSISSDCPAIVFFIEHYQPNLID
                     KLAPIASPMVAMTRIVRKKYGNDSKVIFIGPCIAKKAESDEVDEVLTFTELRELFKER
                     KLIPEKITPSDFDPPHACKGSIFPVSRGMLQTIELSEDILTEDVIVASGKNNFREVIK
                     EFENDLDSNHHLELLCCEGCIMGPGTTSGGKRYSRRSIVSNHVRKKLENFDFEQWERD
                     IEEFSNLDLTQKHNVLDRRIDVPDEAEINKVLVDLNKLTPQDHLNCTACGYDTCREHA
                     IAIVQGLAEVEMCLPFTIEKLHQSVMDLNESNKKLASTQQALRQSEKLASMGQLSAGI
                     AHELNNPLGVITVYSNILKDEAPEDDPIRKDLELIVEQAERCKKIVGGLLNFARKSQV
                     CLVETNMVDFVKQSLTSIIKPENVELIFEYNTKKPFAYIDVDQMMQALTNLEKNAVEA
                     MPQGGKLTVSLTDNLYNVIISISDTGIGIPKENLEHIFTPFFTTKDMGKGTGLGLPLI
                     YGVVKMHRGKIDVKSNTDPAEGPCGTTFTITIPNDLGQ"
     gene            14161..14547
                     /locus_tag="PK345_05180"
     CDS             14161..14547
                     /locus_tag="PK345_05180"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HPX34442.1"
                     /translation="MKKTVLIVDDDVDYLFQIKLKVGQFGFKVITAESQREAEKVLET
                     FRPDLAIFDLMMEKEDSGFILSYKIKQKYPDVPVILATAVTAETGMTFHAAGGESESW
                     IKADLVLDKGIRTDQLEKEIKKLLKL"
     gene            14550..>15457
                     /locus_tag="PK345_05185"
     CDS             14550..>15457
                     /locus_tag="PK345_05185"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HPX34443.1"
                     /translation="MATLKILVVDDEPGIRSGVERILRNFKVDYPFMDDFYDFLIINA
                     ETGEEAIALINSEMPDIALLDNKLPGIQGVEVLEYIRKHNYDITVAMITSHASLDLAL
                     RVTRDGAFDFIPKPFTPQELKSSLENITKQIFLKRMARGMKHEGKQVRFQFLSVLSHE
                     LKAPLTAVEGYLTMIKERQLGNTLEDYDEAIERSLKRLQGMRNLILNLLDLTKIESGK
                     PSQKIQNTNLVEIIKTSIDTMMPFAIQKDVKITLQAPEKVVKYLDPEEMEIIFNNLIS
                     NAIKYNKTGGTIEINLTEESGKTIIKV"
BASE COUNT         4720 a         2966 c         3063 g         4708 t
ORIGIN      
        1 ttatacactt actacccttt tccccggaat aggatatcag gcgttcttta tcaacccttt
       61 aacaattaac ttccctgaat atgagggctt ccttatcaat caaaaaaact ctttcacaaa
      121 ttgtaaaaca aaccctcctt ggaatattca tccgactgac cattatcatc tcttaagtat
      181 tcaaagtgaa tcattgtata agataccaaa tggctttttg ggcatctttg atgagaaaaa
      241 taattgcgtg ggcttttcag aaattactga tgacaagtcg gataatttgt tgttaatagc
      301 ttatggagat gatgaatgga ctttggaaaa agatggcgca cttgaaggcg agccaatgac
      361 ctttaagctt tatcgcccaa gtaatcagac tgaatttatg ctcaatgtaa catatagcga
      421 taacgctcct aaccataatg gcctttttga atctttcggc atgtcaataa tcagcgatat
      481 aactatgtgt ggcgctgaag atgtatcaca cgagatcaat aaaattgtta tctatccaaa
      541 tccttcacaa ggtattttta ctgtttcttc tgaacaactt ttgggtgatg tcaactgggt
      601 agttaccgat gccaaaggac agattataac aaatgggaaa ctatccgatt cgcagcagat
      661 taatttgtgt gctcaaatta aaggtatcta ttttataaga ctaaccggag aggatattat
      721 acacaccgaa aaacttgtga tatggtaggt aatagggtgt tagtagcgtt ctgcccctcg
      781 tcattataca ttatttaaga ataaattagc tgatgtattt ttatttacat acagctatcg
      841 tatcactcac tcttaaagtg gtgttatggc ttgggttccc ttacagttcg gccatgatat
      901 cttctgtgag ctcaaggttg tgaaatactt tttgaacgtc gtcgtcgtct tcaaaagcct
      961 ctattgccct cattattttt tttgcacttt ccagatcaag tgtaactgtg tcgttgggca
     1021 cgcgttgtaa ctcagcaatt tccacttcag catctaactc ttctaatttc ttttgcatat
     1081 tcccaaaatt ttccatcatt gtggaaacag taatagtatc ttcttctatt tcaatttctt
     1141 cagctccggc atcaatcatt tctaattcga aatcgtcaag aatgagcccc tctttctttg
     1201 gcagaacaaa tacgccattc cgttcgaaca aaaagctaag tgaacctgat gttcccagcg
     1261 tgcctccata tctggtaaag atagagcgga tattactaat ggtacgctgt aaattatcag
     1321 tctgacattc cactaccacc gcaataccat taggcaagta gccttcgtat gcgacttctg
     1381 taaaggaaga agcatcttta tctttacctt tgttaattgc tctttcaatg ttatctttcg
     1441 gcatgctagc gccctttgcg tttgttatcg ccatcctcaa acgaggatta ctttcaggat
     1501 cggggccgct ctcctttacg gctatggtaa tttctttgat gatcttagaa aatatttttg
     1561 aacgcttagc gtccatagcg ccctttttac gcttaatggt tgaccattta ttatgacctg
     1621 acatattatt atttttttaa gtttagcctt taaattggct gtaaaaataa tgctttttag
     1681 atttttatat tttctttttt taacaattct ttttgcagta gttataaatt tcaaactgtg
     1741 accttacagc tatttctgaa tcagttttcc ggtatcggtt tatattttca gcgcttatta
     1801 gccttgcttt ggtgttgtcc tgaagttgga tttgcaatat tttccaaata tcgtcctgta
     1861 tttgtggatc ggtaattggg gtggttactt cgatacggta atcaaggttg cgttgcatcc
     1921 aatcggctga ggagataaaa aactcacgat cgccgttgtt gcaaaagaca aacaccctgg
     1981 catgttccaa aaatttatct acaatgccga aggcttctat attttcactc atccctttta
     2041 cgcctggaat gagtacacag atagcacgag caataatttt tatttttact ccggcgttac
     2101 ttgcttcata aagcttgtaa gttagctttt tatctacaag gctgttgagt ttcagaacca
     2161 tccaggcctc tttaccctct tttgcatttt ttatttctct attgatgagt tttaaaaaga
     2221 aatcccttgt ttgaaatggc gaaacgatca gctttttaaa ttttgggata aaatagcggg
     2281 attcgagtaa ctgaaagacc ttgttaacat cggttgcaat atcctgatcg gcagtcagca
     2341 gacttttatc cgcatatact ttggctgtgg actcattgaa gttgcctgtg ctgatgttag
     2401 cataataaac attcgactgg ttttcttgtc ttcggataag aatcagcttg gcatgtactt
     2461 ttaaaccagg gatagtcggg aggattttaa cgccagtttt ttgaagtcgc tctacccagt
     2521 aaatattggc ctcttcgtcg aaacgagcct gtatttcgag aaacacagtt accggttttc
     2581 cgtttctcgc agcatttatc agtgcgttca ttacgttgga gttgcttgca gctcgataaa
     2641 atgtcatttt tattgatcgt actgccggat ctatagaggc ttcgcgaagc aaatcaacga
     2701 tatattgaaa actctgatag ggataatgta gcatgatgtc gcgctttctg atctctctga
     2761 atatgctcga gtggattgtg agacctttgt gcggcagagg ttctgtaggt ggatagagta
     2821 aatcgggcga ccccactatc ggaaacgaca taaaatcgcg gaaattatga taacgaccgc
     2881 ctccacgctg atgatcatcc tcttgaatat ttaacttttg ccttagttct tttaaaaagg
     2941 gtaacgggat tgaattatca tagacaaatc ttaccggctc gccttgctca cgctgcttaa
     3001 tgctctcggc catgatctcc ataaagctct tttgtacgtc gctatcaaag tctaactccg
     3061 agtcacgggt tattttaaaa gcgtaagaat ggtaagtatc gtaaccaaat attgagaaaa
     3121 tatactgtaa tccatgtctc actacatcat ccaacagcat gatgtaaatt ttgtcatcta
     3181 tttcaggaag aataagaaat cgtgatacct gatcggtggg gagctttatc acggcaaaat
     3241 cttctttttt tggattaacg ctcgaagtca tatatacagc aaggtaaagc gcacgatcct
     3301 caatgtcgtc agttgttttc agattttcca tcataatagg gaaaagaaac tgacgaatat
     3361 tgtttaggaa atagtcagta acaaattttg attgtgtagg tataagttgt tgttcatcaa
     3421 taattaatat attcttctct tttagtcttt caacgatctc gacataggtt tgggtaaaaa
     3481 ctttttcttg cgcttcaaca gttgtatata tctgattaag tacctgtttc ggcgaaaatt
     3541 taattctata gtcaagctgt tcaactttta acatccgatt cagtgtagca acacgtactc
     3601 tgaaaaactc atcgcggtta tttgaaaaaa taccaagaaa tctcaatcgc tcgattaaag
     3661 gcgttgatgg atccctcgct tcttgcaaaa cacgatcatt gaaatgtaac caactgattt
     3721 cccgatttat catcggatat tttttttcac cctttgtcat aatcactatt ctttttttaa
     3781 cattgacgga gttattaaaa aattcacaac atacccagcg ttacttattt ccggccaact
     3841 atcggtatca aaacttatgc tgacaagccc ggatgtgggc agccattcaa ttttctgctc
     3901 aaggtaatgg ttcacaaaat aggttagcgt tggattatgt ccaacaagca ttactgtatg
     3961 taaatgatcg gatatgtcaa aaagctgagt aataatgtta tcttcatcag cgctatataa
     4021 agatttgcta atatgtatct tattaatagg atattttacg gcgttagcta ctatccttgc
     4081 cgtttctaac gccctgacgg cggcactcga gataattaga tcgggggtga tattattttt
     4141 gtttagatat cgggcaatta agtttgttct atttatccct ttttctatta aaggccgttc
     4201 tacatcgttc agatgagaaa aatcccatga ggactttgca tgacgtaata tataaatcgt
     4261 tttcatgaaa ggatgatttg taaatttgaa aaggttcaaa gttaatgttt ttgcctcaat
     4321 caagggaaag aacgttgaag gtttatctaa gtgttttcaa agacactaaa tcagcaaacc
     4381 tgtctatagt aaaaaatttg tcgaaaaatt cccgaattgg caaaaaaggt tcgttatata
     4441 aattcaactt tcacctcttt caaaaacttc tcttccaata ttgctgcgat acgttttacc
     4501 accgttcggc gtatttcaag gtccaccggc aagcttgggt cttgatgtgc aatattgata
     4561 cgggtaccca caatgaaatc aatttcgtcg ctttgtagta tcatgtttac tataagccct
     4621 gcaggtcctt tgtcaagcat tgtattttta ttgaaatctt ttaaaaggtt taatactttt
     4681 gttagggtta ggattccttc ggttatcagg tcaacaccat ccatgtagga tatgggaggc
     4741 agctcgggat caatgaaatc aaaagaatct acgattgttt tttcccattc gcgagctata
     4801 atatcggctg ttgtagcgcc acaaagtact tttttcccaa caaaatcagc tactttttta
     4861 gcataaactt tatcgttcac ctcttcataa ggcgggccgg tacagatcag tagttttcgt
     4921 ggatcacgga aatatatcac cgcacagctt gcatcatctt tcaactcata gctatcgttc
     4981 tgataagctt tttttactat gtgttctcca agttcctgag ccgatatcag actgtttcct
     5041 tttatctctt tcagggtgaa atcaatcagt tgttcgcgct cccaaccaaa agggaacgaa
     5101 tcagttccaa ggcctgactg aacaatacca tcggaacaaa agatgattcg atcttctttt
     5161 tctgccacaa aagaacagct acgcaactcc ttatcttctc ctttttctga tgaaagaaac
     5221 atatattgcc atcccggatc aaatacttga ttgtttctga cgataaaagt ttcggggttg
     5281 tcatattcaa ggatgttgat cactcccccc acctctatat caacaatagt aaatgtggca
     5341 tagctgattt tacgcacgct gcataccggc aacgttttca ttatgatctc ggctattttt
     5401 tcgaagtcct tgtgctcagc agtaaaattc atcgccatag tagctgtgag ggtagcaagc
     5461 atattggctt taactccgtg ccccatgcca tcggaaagaa ccacaacaat acgattctct
     5521 tctttgacac gcttttgcat gaaaacgtct ccgcaaatac gctccccatg atggttgcgc
     5581 tgctgacaat tgatctctat aaaaaattcc tggcgtgaca agttgaagaa gttttaagtt
     5641 atcctatttc tgctttttag ttttatgcaa ctgtattatg gagttaagca tagcttcggt
     5701 tttgctggcg ccctcgccga gcaaataacc gatttgctgt accatcccaa ggttttgatc
     5761 aaccacttca gtaattcgct gaattatttg ctcattacgt acttcgggcg aaaacatatc
     5821 gcgaacaata gcgcctacca ccttattctt tttaatggaa aaaacgctta cattaagcaa
     5881 cgactcttca atttccacat cgcggctgat tatattttct ccagtttcga gcgtgttctg
     5941 aaacaattta taaaactgaa caggaactaa ggttttcagg tcggcgccgc gtaagccagg
     6001 gataatatca tcaatctctt ttatgtcatc acccagcaat tcaataaaac tgcggttgga
     6061 tgaggtaact ttaagccttt catccacaat tactacaccc gaaggaatct gattcataat
     6121 ctcacgagag gtttcaagtt tgtcggttgc aagatcatac tcacttttta tttttttcaa
     6181 ctcttcttta taggcattaa cctcctcctg gcttttctta ttcgactccc gtaatgtatt
     6241 gatgtatttg tttttacttc taagcgaaaa atcgtaacag atgtcggttt tagtaagccc
     6301 ttgagctatt gccctggcaa aatctttaca ggtcttaaaa ccacaccctc cacatccggt
     6361 aaagccatcg tcggcctgcc ctaaaaggtt cataatttct tccaacttag cctgatcagg
     6421 ctcttgcaat ctttgatcgt catccttaaa gacccttttc atattcatct tggcatgttt
     6481 tgccatttca acttcccatt tttcaacatc gaagtcctta agacgtttct tagcgtattc
     6541 tgttaccaat gcaatgcgca gttgcttgtc ggcagtagta gccatgcccg gccccattac
     6601 acatccctca tcataaaaaa cattgaaatt ttttcttatc tgatctatat tttggtcgaa
     6661 gctcaacact gcattgagcg atgtttcagc gccttcagca ataattacat cgccatcggt
     6721 aatagcatga tttatacctc cagcctcaag tatcccctca cttgcaggat ataacgagcc
     6781 caattttccg ataggtggat tgaaatcaga aaactcaaga tggctctcat gaatattttt
     6841 ttcactgaac aatttgcgta actctctaaa cgtcagaaca gaatcaattt ttgtaacccc
     6901 atcggaacgt aatatctcac gcttggccgc aatacaaggc ccaatgtaaa caattctgac
     6961 atcctcaccg taagtttctc tcacaacatt tgcagtggcc attacagggg taataatagg
     7021 agctatattt cctatcagcg agggaaaata tttttctata taataaacaa tagcagggca
     7081 attggcggta atgtaatatt tccctttttt ttcgtcgaat agcattttat atgctttagc
     7141 caccagatca acgccaaatg acacttcatt tacatattta aaccctaacg atctgatcat
     7201 ttctacaaat ttcctgtaat cggtgatatc aggaaactcg cccgagatgc taggatcaac
     7261 tatagctgca acgttttctt ccgatttcat taactcctta acctcgccag tcgaatccca
     7321 ataggaaatg gcatcatagc ggcatgcacc aaggcaactt ccgcaaccaa tgcaacgtgc
     7381 atcaactacc gtcgggatat cgtttccgac tgatgtctgt atagcatgaa ccgggcatgc
     7441 ccttacacaa gcaagacagt tggtgcagtt tttaggatca attgaaatta atgccatgtt
     7501 aaaaagtgtt ataataactc attttcaaga atttccagaa ttgattcagc attaatatgt
     7561 tcatatctct tctggtctat cataatacta gggccatttt cgcaattgcc aaaacaatgc
     7621 ttgcccttaa aatttacttt tcctgaaaga ttatgctcct ccagaaattt ttttatcagt
     7681 tgtaccattt gtttgttacc tcttgcaaaa caagagcttc caagacaaat aataacctct
     7741 ttttgtaagg taactaatgc cattttcaaa taatattgag tgcaaaggtc taaaaaaaga
     7801 tgaaataaaa gaatttgcgt taataattta acaatgtgaa ttgaacaaca aaaaataact
     7861 ttatttttgt tcatctgttc aaaacatctt aatattaaat ggaatgacag aaataaaaac
     7921 cgttttacag gactatcaaa atcgagaacg cgaatatctt atacctattt tgcaggatat
     7981 tcaaagtctt atcggtttta tatcgcccga cgccattgcc gctgtagcgt tacatcttaa
     8041 aatacccacc agcaaagtat atgcagtagc ttccttttat gatcagttca agtttttacc
     8101 atttgcaaaa taccgtataa aagtatgcaa tggcaccgga tgtcatattg agggttcagg
     8161 cgctatctac gatgaattgg tgaaactatt gggaggtgaa accagaactg agcttttcag
     8221 cattgagagg gtacaatgtt taggcgcttg tggtaatgct ccggttatta agattaatga
     8281 taaattttat atcgctgtta ctttggaaat ggcagctgat ataattaaat ccattaaagc
     8341 tttggaggat gcgccatgat tgaaaaagga caagataccg gtttaaccgg ttatttgatc
     8401 gaaaaggttc taaagaatta ctctggcgat tatccgcctg acgtgaaacg cgagattgat
     8461 ctgttgttac gtaggcattt gcgccgtccg gtaatttatg ttggcacagg cacctgtggt
     8521 attgcagccg gcgctgatgc tactcttaaa gcagcaaaag aatatcttaa aattaatcag
     8581 ttggaagcag atcttataga agtaggttgt gttgggttgt gcagcgctga gcctcttctc
     8641 gactatcagg ccccaggtag agctcgtgtt agctttcaac atgttacagc ggataaagtt
     8701 tacgaaatat tagaagaatg ttttcataat catatttcag atgaacatgt attagggcaa
     8761 gtggcaagtg aaggacatga gttatggcat agaataccgt tgattcagga gaatcctttt
     8821 tttaaaaagc aacatcggat attgctgtat gattgtggcg aaatttcgcc gaccaacctt
     8881 gatgaataca ttgcacgcga tggttataaa gcatttttaa aagttctgat gaattatccg
     8941 cccgaggagg tttgtaatat tgttggacaa agtggattaa aaggacgcgg aggcggtggc
     9001 ttccccacgg caacaaaatg gaataatgcc agattaatgg ccagtgataa gaaatttgtt
     9061 atttgcaatg ccgacgaaag cgatccggga gcttacatgg atagagcgtt agtagagagc
     9121 aatccctacc gcttgattga aggaatttcg atagcagctt atgcggtggg cgccagtaaa
     9181 gcctatattt acatacgttc ggaatataat ttagctgtaa aacgactcca aaattcactt
     9241 atcagagcta aagaggcagg atttttagga gaaaacatat ttaatagcgg gtttaatctt
     9301 caaataatct tgcgtcaagg gccgggcgct tttgtttgtg gcgaagaaac agctttgata
     9361 aaaagtatcg aaggccatag aggtactcca gagaccaaac ctcctttccc tttcgaaaaa
     9421 ggactgttcg gtaaacccac tgtggtaaat aatgttgaaa cacttattaa tgtcccgctt
     9481 atcctacttc atggcccttt gtggtttaaa tccatcggaa cagaaaccag taccggcacc
     9541 aagctgtttg ccttaacagg ggatactgta aatacaggtc tggtagaagt tccatttggg
     9601 ataactctcg aagagattat ttatgaaatt ggcggaggta ttaaaaatgg agcgtcatta
     9661 aaagctatac agatcggagg accgtcagga agccttgtgc ctgctgcaca tagcgatctg
     9721 caaattgatt atgaagcatt tcataaaaga catcagatca ttggatcagg cggtatggta
     9781 gtaatggatg agaatacctg tatcctcgat atgataaaat atttcatcaa ctttttgcaa
     9841 aaggagagtt gtggcaagtg cataccatgt cgtgagggaa caaataggat gtatgaaatc
     9901 ctacagatga tcaccacccg tcctaagcaa aatgataaac acgaaagcct cacgcggttt
     9961 cagggcgtta tccagttgga aaatctagct aaagtgataa aagagacctc tctttgtgga
    10021 ttaggtaaaa cagccccaaa ccctgttcta agtgctttag agtggtttag agaagagttt
    10081 gaagaacatg ttttcgaacg aaaatgccgg gctaatgtat gcaaaaacct tattgaatat
    10141 gtcattagcg ccgagcgttg tacaggatgt atggtatgca tgaaaaaatg cccttatgat
    10201 gcaatcactg gcgcacccct tgaaccacat ctgataattc gcgataaatg cacaagttgt
    10261 ggtatctgtt acgaagtttg taagttctct gctattgatt tcatttaatt aattcaaata
    10321 attggaatta aataattaaa aagaatatcc gatgattttc aacatcgaag taaataataa
    10381 agttttaaag gccaagcgcg gagattcaat tcttgaaacg ttgaatcaga acggcatttc
    10441 ggtgccaacg ttatgccgga tgcagggttt tccatcatcc ggcgcttgtc gtttgtgtgt
    10501 tgtagaagta gaaggtaaag aggacttagt gccggcctgc tcacacaacg tagaggaatg
    10561 gatgaaaata catacacatt cgcctcgagt aatcaatgcg cgccgaacaa ttgttgaact
    10621 acttctctca agccatcccg acgattgtct ctattgcatc agaaacaaaa attgcgaatt
    10681 gcaggaatta gctgacgagc ttggaataag agagaggaaa ttgcccatag taaagaaaca
    10741 ggtaaaaaaa gacccttcaa gcgtaagtat tacccgcgaa cccgataaat gcatactttg
    10801 cggtcgttgc ataaaagtat gccgtgactg gattggtatc tccacccttg attatattgg
    10861 taaggcaatg aacacaattg tcggccctac attcgacaat aaattaaata tttcaaattg
    10921 tatcacctgc ggccagtgta taatggtttg tccaaccggc gctttgcacg aaaaggatga
    10981 tgtcaataag gtgttgttgg ccttgcagga tcccgataaa tatgtagttg tacaaatctc
    11041 gcctacggtc tcagtttcac ttgccgaaga gtttaatata cggttcggta aggaacttga
    11101 tgaattatta gtagctactt tgcgacggat gggttttaat aaagtgttca aaactgcttt
    11161 cgcagctgat ataatgctaa ttcaaatggc tgaagagctt attgatcgaa tggagaaaaa
    11221 tgaacgactg ccaatactat cgagcaactg cccagcattt gtcaagtata ttgaacaaac
    11281 ataccctgac cttatcccaa atctttcggt gataaaatcg ccacagcaaa tcatgggaag
    11341 ccttgttaag caccattttg ctaaagatca aaaagtgaaa gccgagaaga tattttctgt
    11401 ttcgcttatg ccttgtactg ccgcaaaatt tgaagcaagc cgagaagaat ttactcacaa
    11461 agggaattca gatattgacg ttacccttac tacaagagag ctggcaagga tgataagact
    11521 ttatggtctg aatattcaac aattggcgcc agaggtttcg gatacaccgt tcgttatacg
    11581 cacttcggca tcaaagatta tcggtaccag tggtggcctt gcagaagctt tgtatcgcac
    11641 actatattat aaattgacag gaaagcaatt gattaatttc aagattgccg ctctgcgagg
    11701 gaataaaaaa attaaagagt acaacagcaa aatcggtaat cataaaatag gaattgctgc
    11761 agtaaatggc ctatctaacg ctgttgaact tctcgataaa attatcgaag gaaagtctgc
    11821 ttatcagttt attgaaataa tggcctgccc gggaagttgt gtaaatggtg gcggacaacc
    11881 aataggatcg gggcaggaag ctataaaagc aagaattaaa acgctttacg atattgatga
    11941 gaaggaatca gtaaaggcat cctacaaaaa tatggcggta acagaattgt ttgacaaata
    12001 tattgataag gaaaaggagt cgtttttcca acagtttatt acttcttatc aaaagagaga
    12061 tgttatgctt taaacttgtg ttaacagcgc taaatatgat tatttttatg ttcggataag
    12121 tattttattt taaatttaaa aaaaataagc caacatgagc cattccaaac gaaaacaact
    12181 tgtttttacc gttaaagacc tttgcagggt ttgttatact tgcgtgcgcg aatgtcctgt
    12241 aaaggccatt aaaattatca atggtcaggc agaagtaatc aatgagcgtt gtattggatg
    12301 tggcaattgt ttcatggtgt gcagtcaggg cgccaaatgt tttttaaaga caataaaaga
    12361 tgctaaggct ataatagctg aaaatgaaaa agtaattgct attgttgccc caagctatcc
    12421 ggccgagttc accgagtttc ccgaccatcg ccaatttgtg gggatgattc gtgcgctcgg
    12481 tttcagccat gtttgcgagg taggttttgg ggctgatatg gtggcaaggg agtatcgcaa
    12541 attacatgat atcacattaa ctggcgggag tatttcgtca gattgccctg cgattgtttt
    12601 ttttatcgaa cactaccagc ctaatctgat tgataaatta gcgcctattg cttcgccaat
    12661 ggtagctatg acaaggattg tcagaaaaaa atacggcaac gattcaaaag taatttttat
    12721 tgggccatgt atcgcaaaaa aagctgagag tgacgaagta gatgaagtgc tgacatttac
    12781 tgaattaaga gagcttttta aagaaagaaa acttatacct gaaaagataa ccccttcgga
    12841 ttttgatcct ccccatgcat gcaaaggttc gatttttcct gtaagtagag gaatgttaca
    12901 aactattgag ctttccgaag atatccttac ggaagatgtg attgttgctt cgggcaagaa
    12961 taatttcagg gaggtaatca aagagtttga aaatgatttg gatagcaacc accatctgga
    13021 gttgttatgt tgcgaaggat gtattatggg gcctggcacc acaagtggag ggaagcgtta
    13081 tagccgtagg tcaattgtga gtaaccatgt aaggaaaaag ttggagaatt ttgattttga
    13141 acaatgggaa cgcgatattg aggaatttag taatctcgac ttaacgcaaa aacataatgt
    13201 ccttgatcgg cgtatagatg taccggatga ggcagagatc aataaggtat tggtagattt
    13261 gaataaatta acacctcaag atcatcttaa ctgtacggca tgtggttatg atacctgtcg
    13321 cgaacatgca atagctattg ttcaaggttt agccgaagta gaaatgtgtc tgccattcac
    13381 cattgaaaag ctacaccagt cggtaatgga tttaaacgag tcgaacaaaa aattggcttc
    13441 tacacagcaa gctttgaggc agtcggaaaa attggccagt atggggcagc tctccgctgg
    13501 aatagcgcat gagctcaaca acccattagg ggtaataacc gtttatagca acatcctaaa
    13561 agatgaagct ccggaagatg atcccatacg taaggatctg gaacttattg ttgagcaagc
    13621 tgaacgatgt aaaaaaattg tcggcggatt actcaatttc gcacgtaaaa gtcaggtttg
    13681 ccttgtggaa actaatatgg ttgactttgt aaaacaaagt ctgacatcaa tcataaaacc
    13741 cgaaaatgtt gagttaatat ttgagtataa tacaaagaaa ccattcgcct atattgatgt
    13801 agatcagatg atgcaagcgc ttacaaatct ggaaaaaaat gctgtggagg ccatgccgca
    13861 aggaggtaaa cttacagttt cacttaccga caacctgtat aatgtaatca ttagcatatc
    13921 cgatacaggc attgggattc cgaaagaaaa tttagagcat atttttacgc cgttttttac
    13981 aacaaaagat atgggcaaag gcactgggct tggtttacca ttgatttatg gtgttgtaaa
    14041 aatgcacaga ggaaaaattg atgtgaagtc aaatactgat cctgctgaag gtccttgtgg
    14101 aacgactttt actataacta ttccaaacga cctcgggcag taaaataata aaattgtaaa
    14161 atgaaaaaaa cagtacttat tgtagatgat gatgttgact atctctttca gatcaaactg
    14221 aaagtagggc agtttggatt taaggttatc acggctgaaa gccaacgtga agccgaaaaa
    14281 gttctcgaaa cattccggcc ggatctagca atattcgatc tgatgatgga aaaagaagat
    14341 agcggcttta tcctcagtta caaaataaaa cagaaatatc ctgatgtccc ggtaatattg
    14401 gctacggctg taacagccga aactggaatg acttttcatg cggcaggagg agaaagtgaa
    14461 tcgtggataa aggccgatct tgtattggac aaaggaatta gaaccgatca acttgaaaaa
    14521 gagataaaga aacttttaaa actttaataa tggcaacatt gaagattctg gttgttgacg
    14581 acgagccagg tatcaggtct ggggtggaac gcattttaag aaatttcaaa gtggattatc
    14641 cttttatgga cgacttttac gatttcttaa ttataaatgc tgaaaccgga gaagaagcga
    14701 ttgctctgat aaattcagaa atgcctgata tagccttact tgacaacaaa ttaccaggta
    14761 ttcaaggtgt tgaggtactt gaatacatca gaaaacacaa ttatgatatc accgtcgcca
    14821 tgatcacctc gcacgcttcg ttagaccttg ccctaagagt tactcgcgac ggagctttcg
    14881 actttattcc caaacccttt accccacaag agttgaaatc ctctttagaa aatattacca
    14941 aacaaatatt tttgaaacgt atggcgcggg gtatgaaaca cgaaggcaaa caggttagat
    15001 ttcagttttt atctgttctg tcgcacgagc ttaaagcgcc gctaacagca gtggaaggat
    15061 acctgactat gatcaaggaa cgccaacttg gaaatactct cgaagactat gatgaggcaa
    15121 tagaacggtc gttgaaacga ctacagggaa tgcgtaacct tatcctgaac ttactcgatc
    15181 tcacaaaaat tgaatccgga aagccaagtc aaaaaattca aaatacaaac cttgttgaaa
    15241 tcatcaagac ttcgattgat acaatgatgc cctttgctat tcagaaagat gtgaaaataa
    15301 cgctacaagc acctgaaaag gttgtcaaat accttgatcc tgaagagatg gaaattatat
    15361 ttaataacct gatatccaat gcgataaagt ataataaaac tggtggaaca atagaaatta
    15421 acctcacaga agagagtgga aaaaccatta ttaaggt
//
LOCUS       DAOOAI010000052        14947 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_5173_length_14947_cov_4.539484, whole genome shotgun sequence.
ACCESSION   DAOOAI010000052 DAOOAI010000000
VERSION     DAOOAI010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14947)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14947)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14947
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5173_length_14947_cov_4.539484"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..614
                     /locus_tag="PK345_05190"
     CDS             <1..614
                     /locus_tag="PK345_05190"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HPX34444.1"
                     /translation="NRSWFEANKDWYLESRHEFIQLTGEIIKQIALFDPSIGLLTAKD
                     CVFRIYRDVRFSHDKSPYKTNMGAYLSRGGKKSRYAGYYLHLEPDASMAAGGKWMPQA
                     NELKLIRLEIFNYPAEFISILNNIEFRKRFTLQVNEKLKKSPLNFPAEFEHVELIKYK
                     SYTVSLSIPDEITLKDELISTVVEAFRAMSPFINFLNRALDEI"
     gene            988..3081
                     /locus_tag="PK345_05195"
     CDS             988..3081
                     /locus_tag="PK345_05195"
                     /inference="COORDINATES: protein motif:HMM:NF014774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HPX34445.1"
                     /translation="MISNLLFSIVLLISVFIFYKSVCRIVRMIKMGKDVEIKDNPVQR
                     WRKMGFVAIGQSKMVARPLAAVLHIIVYVGFFVVNVEMLEILIDGITGSHRALSFIGN
                     LYPFFISVFESFAFAVIIACLIFLARRNIIKLKRFWNKEMTSWPRTDANIILITEVLL
                     MCSVLVMNASDSILQSRGYTHYSAVGNFFISDFIKPVLVGMNSDSLASLERFTWWFHI
                     VGVFIFLNYIPYSKHLHVFLSFPNVYYSKLGPKAQIQPLDSITREVSLMLNPEMEISA
                     TYVEGAEMELFGAKDTPDFTWKNLMDAFTCTECGRCTSSCPASITGKKLSPRKLMMDL
                     RDRTEELEKIGIKSKKECEDGKTLFDRITPEEIWACTTCNACVQECPVNIDPVSIVLE
                     LRRYLCLEKSAAPRDLNIMFTNIENNGAPWQFSSEDSLKWATDAGIEVPLMANLFSNG
                     KKPEYLFWVGSSGAFDDRFKKVSVKFAKILNHLGIDYAVLGSEETDTADSARRAGNEM
                     LYQMQAMKIIETFKNYEVKKIITCCPHDFNTFKNEYPAFGGNFEVIHHSQFLNNLISE
                     GKIKLNDNLFEGKKITFHDPCYLGRGNDEYEAPRSVIGAINGISFIEMKRSRHLSLCC
                     GAGGGQMFKECEKGNKEIFIERIEEVIKSGADIIATACPFCMVMMTDGIKYKNMEDNL
                     KNYDIAELVGLSMGI"
     gene            3277..4440
                     /locus_tag="PK345_05200"
     CDS             3277..4440
                     /locus_tag="PK345_05200"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPX34446.1"
                     /translation="MNTKKITFITITPVLFSFFVMSFCDLVGIGVDNAMADFQLSNIM
                     AQLIPSAVFVWFFFLSVSIGVLQDRIGKRNMVNIGMLVTAAGLLLPYFFYSFPLLLAG
                     FVLLGIGNTILQVSANPLLIDVSPSGKSASLLSFSQFIKSIGSMVAPFIASYFALRFG
                     NWKLVFLLFGLFSIFNAVWLHFTKVEESQSTERKATFGSAFGLLNTPFILLMALGIFF
                     VVAIDVGINSTSGQFLMEKLNIDPEPAKQGRSLYFFGKMLGTFVGAILLTKLHSGKFL
                     IYSSIATLITILAFIFSPSSVFAFTLMFLIGFAASNIFPLIFVITVNRYPKRSNEISG
                     LMIMAVSGGAFIPPLVGKISDISNVTKGMYVFVGCAIYLLFVAAFSLRKISNQ"
     gene            4470..5810
                     /locus_tag="PK345_05205"
     CDS             4470..5810
                     /locus_tag="PK345_05205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007899177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPX34447.1"
                     /translation="MKSKIILLTIVLFITNATHSFGQTKIDSEEIADKMQWFADAKLG
                     IFIHTGIYAVNGITESWSFFNKRISHDDYMKQLNGFTMSKYDAAAWADLIKASGARYA
                     VITTKHHDGVAMYNTQMNDLSSLKATPAAKDIIKPFYNELRQRDIKCGAYFSLLDWSH
                     DNYPGFLRDSGRYEIKDDWERWYRYRDFFQGQIKELLAGYNPDLWWFDGDWELSAERW
                     EAEKIRQMILTHNPNAIINGRLQGYGDYATPEQNFPVVRPKHNWWELCMTTNNSWGFQ
                     FKDQNWKTPYEVITIFADVISNGGNLLLDIGPKEDGTFPPEQINILKELGQWNKKHSE
                     AIFNSLPGLPPGHFYGPTTLSKDSTNIYLFLAGNPQNQIVVRGLFDNIENVSIVGSDI
                     SLEWKIVGKISWSLVPGLVYIQVPEKYQDQYMTVIKIKLKDKVHLYRGEGGLDI"
     gene            5823..9149
                     /locus_tag="PK345_05210"
     CDS             5823..9149
                     /locus_tag="PK345_05210"
                     /inference="COORDINATES: protein motif:HMM:NF013300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004560 - alpha-L-fucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-L-fucosidase"
                     /protein_id="HPX34448.1"
                     /translation="MKFIRSAVIFIFIIVSLELFAIGSDTLYFNSHNREVVVTDPTQG
                     INSYTKWVKFPSQELDIRKITMYVTFGCPDTMRCADWDYLDHIMVQRVGGINSSDLNW
                     EIGRIITPYGGFFDNDWQFKWQADVTDFSMILRDSVEINYIHTGWEPNNDRGWLISID
                     FEIITGTPVAVPISITEIYNDHFPYGDSLKPIDQTLLPKSFITETNTDFVRLRVLQTG
                     HGMDEPDNCGEFCSKYREIYLDDNLVQKRQMWMTCGDNPLYPQAGTWIFDRANWCPGY
                     LVQPEIFDLATTSGKEQIIRFIMEPYTATKQNQGKQVISAYLIQYQQLQHVLDISIED
                     IVAPSIKDIYLRKNPAAANPQIILKNNGKETIYSATIIYKTEGFKEQKEEWRGELPVG
                     SSELITLSGLVNFSSGQNIFTAKVQNPNGKADQYPDDNVLSTTFNSPPVHDTLLVFRL
                     LTNNQPEHNSWKLISHDGKVVVEKRANELKARTLYSDTLRLSQGAYTLLFSDSAGDGL
                     EFWYNSGGGRGEAWLLDGNDNLIKAFEPDCGLGWIYNFTVSQNPDPINRQNKAISLYP
                     ARTSDFTKLTYFANHQKDIIVKLISDPGGEVVEERNYPNLKKGIFDFDLTRFSYGRFF
                     LKVFADGQEIFNKRIRYVEPAKEPDADFYVWPTDPLVSQKLHDWQDWKFGVIIHWGPY
                     SEWGVVESWSLCPEDEPWCIRQGPFAHDYYTYVQEYEKIQQTFNPQKFNPDKWADACK
                     RAGMKYVIFTTKHHDGFCMYDSKYTDYKITGSESLFAQNPKSNIAFEVFEAFRKTGMR
                     TGAYFSKPDWHNDDYWWPYFPVFDRNVNYAPSKYPERWERFRKFVFNQIEELMGNYGK
                     IDILWLDGGWVRPVKTASNESNQEIIEKGYNQDIDMPSIAKRARQLQPDLIIVDRTVH
                     GMYENYTTPEQEIPDHVPNHPWESCITLGDSWYHTGPEENYKSLTWAVHTLIKIIAKG
                     GNLLLGIGPDKTGDFAPEVYTRLEEIGNWINVNNEAIYGTKPLAPYHHGNLYFTQAKD
                     EKTKFAFCLKEESDNFPNEIKLPFGVDIRKAKIKLLGYKGSIRATSIQDETIIKLPKL
                     TTKLKNSPALVFSISITNQY"
     gene            9334..10053
                     /locus_tag="PK345_05215"
     CDS             9334..10053
                     /locus_tag="PK345_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPX34449.1"
                     /translation="MIEKLSIIIPAYNEGRTIHLILNKVKEVNLINNITKEVIIVNDC
                     STDETESIIENYIEANPSFNIQYFKHDINMGKGAALHTGISKATGQYLIIQDADLEYD
                     PQEYNDLLKPVVNGFADVVYGSRFIGGNPHRILFYWHTIGNKFLTALSNMFTNLNLTD
                     METCYKLFNTKLIQSIHLKEKRFGFEPEVTAKIARIKGIRIYEVGISYYGRTYKEGKK
                     VGWKDGFRALYSIIKYALLKK"
     gene            10353..10874
                     /locus_tag="PK345_05220"
     CDS             10353..10874
                     /locus_tag="PK345_05220"
                     /inference="COORDINATES: protein motif:HMM:NF012787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HPX34450.1"
                     /translation="MNKSFLLLATFLSFFLLSQNIIAQDITPNKKMYGKSFYHQKAPE
                     FVVEKWLSDVPDMKGKFIMIDFWATWCGPCKRSIPQINEWYNKYKDRMVVIGVSDEPE
                     AKVLALKEPKKEYYSAIDTKKRMSKTYEVSGIPHIVIIDPQGIVRWQGFPFLAGYELT
                     DEVIDEILEKYSK"
     gene            complement(11019..11954)
                     /locus_tag="PK345_05225"
     CDS             complement(11019..11954)
                     /locus_tag="PK345_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008768242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPX34451.1"
                     /translation="MKKRILVTGGAGFLGSHLCERLLSEGNDVICLDNYFTGQKQNVV
                     HLIGHPYFELVRHDVTMPYYVEVDEIYNLACPASPIHYQYNPIKTVKTSVMGAINMLG
                     LAKRIRAKVLQASTSEVYGDPEIHPQHEEYWGNVNPIGDRACYDEGKRAAETLFVNYH
                     KQNGVRIKIIRIFNTYGPRMHPNDGRVVSNFIMQALQNKEITIYGNGQQTRSFCYVDD
                     MVTGMINMMDTDDKITGPINIGNPEEFTIHQLAETVVRMTGSKSKIVYHPLPSDDPLQ
                     RKPDISKARQLLGWEPKIKLEEGLMKTIEYFRNLL"
     gene            complement(11974..13302)
                     /locus_tag="PK345_05230"
     CDS             complement(11974..13302)
                     /locus_tag="PK345_05230"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HPX34452.1"
                     /translation="MRITIVGSGYVGLVTGTCFAELGIDVTCVDIDIAKIEKLKKGIS
                     PIYEPGLDDMLKRNIERGKLSFSTDLTPLVNESEIIFSAVGTPPDEDGSADLKYVLNV
                     AKTVGRSMKKHLVFVVKSTVPVGTSKKVRNTIQEELDKRGLKINFDVVSNPEFLKEGA
                     AIDDFMKPDRIVIGVESEFAKGVMSRLYRPFTLNNHPVLFMDVPSAEMTKYAANAMLA
                     TRISFMNDIANLCDLLGANVNMVRRGIGSDSRIGSKFLYPGIGYGGSCFPKDVKALIK
                     TGEEYNYKLRIIQSVEDVNEDQKVALFKKLLDYFDGNLKGLSVGIWGLSFKPQTDDIR
                     EAPSLVIIERLLKAGCKVKAYDPVAMENVRQIFGDSIEYANDPYDAITNVDALLIVTE
                     WIEFRFPKYKLMQKLMAKHVIFDGRNIFDRNEVKKAGFEYYCIGANVNSH"
     gene            complement(13521..>14947)
                     /locus_tag="PK345_05235"
     CDS             complement(13521..>14947)
                     /locus_tag="PK345_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34453.1"
                     /translation="AGWSAHISCHYLQGLPECASSPSPTNNAQNVSLNSNLSWVSYDA
                     VTFDVYFGLVPDPPFVETVPTPLYTPVLEPNTTYYWKIIPINANGQASDCPVWSFTTS
                     GPEYYMSNGTVTVNSGVFYDSGGPNANYSNHENYTMTFLPIDENTPLKFTFTQFDIEN
                     NWDYLYIYNGPNTTATQFPGSPFTGTYSPGTITSTHPTGAITFRFTSDYSVVKSGWIA
                     NFITVGNLACNITANPEEICQGGYSLLNCNVSGGTGNYNFNWSPASSLSNPNVQNPIA
                     TPNETTTYTVTINDGATTISDNYTLVVHELPPVNLGEDVTVCETETVTLDATTPNAVS
                     YLWTPGGYTTAVIQVDTTGVGIASVTYTCQVTDVYGCVGQDDITITFKSCVGINEISD
                     NISMLVYPNPAKNLLNISFTGVTDEIEYALLNYHGQQVYSNYIGKLNGSVSQEIRLDN
                     LARGVYYLRINSSDGAYIKKVVLQ"
BASE COUNT         4681 a         2732 c         2883 g         4651 t
ORIGIN      
        1 ataatcgttc gtggtttgag gccaacaagg attggtatct tgaatcaaga catgagttta
       61 ttcagcttac cggcgaaata attaaacaaa ttgctttatt cgatccatcc attgggttac
      121 tcactgcaaa agattgtgtt ttcagaatat atcgcgatgt taggtttagc catgacaaaa
      181 gtccttataa gacgaacatg ggggcttatt taagccgtgg cggcaaaaaa agtcgttacg
      241 ccggatacta tctacacctt gaacctgatg cttcgatggc cgccggtgga aaatggatgc
      301 ctcaagctaa tgagctaaag ctgataaggt tagagatttt taattaccca gcagagttta
      361 tttcgatcct aaataatatt gaattcagaa aaagattcac cctccaagtt aatgaaaagt
      421 taaaaaaaag tcctctcaac tttccggcag agtttgaaca cgttgaatta atcaaataca
      481 aaagttacac agtaagcttg agtattcctg atgaaattac tctgaaagat gaactaatta
      541 gtactgttgt cgaagcattc agggcaatgt ctccttttat taattttttg aatagagctc
      601 ttgacgagat atagagaaga taataaaaaa agcagtaaat attaaatcct gaattaaatg
      661 attggtcagg atttcagttc agtatgatcc gactttaaca attgaaagat taaacaatca
      721 aaagtcggta aacggcacaa tggaatttgg aattaataga ggaactaaaa cgatatcgcc
      781 tccttagatg actattacaa cccaacagcg ctttcaattg attaattcat gataatttac
      841 attgtgttta aattgcactc atacttcgct gtatatcatt ttattacaat tattatcctc
      901 aattaataag agtagatatc agcagaaatt ataatattgt aacttgataa aaacgtcaat
      961 attaacaatc tgtatttaac tgataaaatg atatctaatt tacttttcag catagtattg
     1021 ctcatatcag tttttatttt ttacaaaagc gtctgtcgca tagtaaggat gataaaaatg
     1081 ggtaaggatg tcgaaatcaa ggataatccg gtacagcgat ggaggaagat gggttttgtt
     1141 gctattgggc aatcaaaaat ggtagcacga ccattagctg ctgttttaca cattatcgtt
     1201 tacgtaggtt tctttgttgt gaatgttgag atgcttgaaa ttttgattga cgggattact
     1261 ggtagccatc gggcgctctc ttttattgga aatttatacc catttttcat ttcagttttc
     1321 gagtcatttg catttgccgt aattatagca tgtcttatct ttctggcgag aagaaatatt
     1381 atcaaactca aacggttctg gaataaagag atgacgagct ggcctcgtac cgatgccaat
     1441 atcatcctga ttacagaagt gcttttaatg tgttccgtcc tcgttatgaa tgcttctgac
     1501 tctattcttc aatcacgtgg ttatactcat tattctgcgg ttggtaactt ttttattagc
     1561 gatttcatca aacccgtttt agtgggaatg aacagcgatt cccttgcttc tttagaaagg
     1621 ttcacatggt ggtttcatat tgttggtgtt tttattttcc tcaattatat tccctattcc
     1681 aaacaccttc atgtttttct ctcttttccc aatgtctatt attccaaact gggaccaaaa
     1741 gctcaaatcc agcctctcga ttccattact cgtgaggtaa gtcttatgct aaacccagaa
     1801 atggaaatat ctgctactta cgttgaaggc gctgaaatgg aattatttgg cgctaaagac
     1861 acacccgatt tcacatggaa aaacttgatg gatgctttta cctgcaccga gtgtggccgt
     1921 tgtacatcat cttgcccggc aagcattaca ggtaagaaac tctcgccgcg aaaactgatg
     1981 atggacttac gagatcgtac tgaagagctt gaaaagattg gaataaaaag caaaaaggaa
     2041 tgtgaagatg gcaagacatt gttcgacaga ataactcctg aggaaatttg ggcttgcact
     2101 acctgtaatg cctgtgtgca ggaatgtccg gtaaatattg atcccgtatc cattgtgctt
     2161 gaattaaggc gttatctctg tttagaaaaa tcggcggctc cacgcgattt aaacataatg
     2221 tttaccaata ttgaaaataa tggcgcacct tggcaatttt catccgaaga tagcttgaaa
     2281 tgggctactg acgctggtat tgaagtgccg cttatggcca atttatttag taatggtaaa
     2341 aaacccgaat acctgttttg ggttggctca tcaggcgctt ttgacgatag gtttaaaaag
     2401 gtaagcgtta aatttgccaa aattttgaat catcttggta ttgattatgc cgttttgggt
     2461 tcggaagaga cggatactgc ggattctgct cgcagggcgg gtaatgaaat gctttaccag
     2521 atgcaagcaa tgaaaattat cgaaacattt aaaaattatg aggtaaaaaa gatcattacc
     2581 tgctgcccac acgattttaa tacttttaaa aatgaatatc ctgcttttgg aggtaatttt
     2641 gaagtaatac atcattctca gtttttaaat aatctcattt ccgaaggtaa gatcaagcta
     2701 aacgacaatt tatttgaagg gaaaaagatc actttccacg atccctgtta ccttggtagg
     2761 ggaaatgatg aatatgaggc tccacgctct gttattggcg ctatcaatgg tatttctttt
     2821 attgagatga aacgtagccg gcatctatcg ctctgttgtg gcgccggtgg cgggcagatg
     2881 tttaaagaat gcgaaaaagg caacaaagag atattcatcg aacgtattga agaggtaatt
     2941 aaaagtggag cagatataat tgccactgca tgtccatttt gcatggttat gatgaccgat
     3001 ggaataaaat ataaaaacat ggaggacaat ctcaagaatt atgatatcgc cgaattagtt
     3061 ggattgtcaa tgggtattta aaaaactaaa ctatacattc aattttaaaa atttgtttat
     3121 tcattgtcag atggttacgt taaaaaaatt atattaaaca aaaacaaata acgatttgta
     3181 tttttgagct ttcgtgattc ggtttaataa aatgtaacca tctttacctt tttaagaaat
     3241 ctttatcgaa acttaatatc aaataattca ctttatatga acacaaaaaa gataactttt
     3301 atcactatca cacctgtact tttctcattt tttgtaatga gtttttgcga tcttgtgggt
     3361 attggtgtag ataatgccat ggccgatttt caacttagta acattatggc acagttaata
     3421 cccagcgctg tttttgtctg gtttttcttt ttatcagtat ctatcggtgt gcttcaggat
     3481 aggatcggta agcgcaatat ggttaacata ggaatgttag taaccgctgc cgggttgcta
     3541 ctaccctatt ttttttattc ttttccgctg ctacttgccg gtttcgtttt actgggaatt
     3601 ggcaatacca ttttgcaagt atctgctaac cctttattaa ttgatgtgtc tccctcaggc
     3661 aaaagcgcca gtttacttag tttttctcag tttataaaaa gcataggctc tatggtagcg
     3721 ccctttattg cctcttattt tgcattaaga tttggaaact ggaaattggt ttttcttctc
     3781 tttggtctgt tttcaatatt taacgcagta tggttacatt ttacaaaagt tgaagagtcg
     3841 caaagcacgg aaagaaaagc tacgtttggt tcagcatttg gcttgttaaa tacgccattt
     3901 attctgctga tggcgcttgg gatttttttt gttgtagcta ttgacgttgg tattaactca
     3961 acctctggtc agtttttaat ggaaaaatta aatattgatc ccgaacctgc caaacaggga
     4021 agaagtcttt acttttttgg aaaaatgctt ggaacttttg tcggggctat tttacttaca
     4081 aagcttcatt cgggtaaatt tctcatttat tcatcaatag ccacgctgat tactattctt
     4141 gctttcattt tttcgccatc atcagttttt gcttttacac taatgttttt aatcgggttt
     4201 gcggcttcca acatattccc cctaattttt gttattacag tgaatagata ccccaagcgt
     4261 tccaacgaaa tttccgggtt gatgatcatg gcagtgtcag gcggggcttt tataccacct
     4321 ctcgttggta aaatatccga catttcaaat gttactaaag gaatgtatgt gtttgttgga
     4381 tgcgctattt atttactctt tgtcgcagcc ttctcattac gaaaaataag caatcaataa
     4441 tttatataaa tcactaatcc taagatacaa tgaaatcaaa gatcatcctt cttactattg
     4501 tcctatttat tactaatgca actcattcat tcggacaaac gaaaatagat tctgaagaaa
     4561 ttgcagataa aatgcagtgg tttgctgatg ctaagctcgg aatatttatt catacaggaa
     4621 tttacgctgt taatggaata accgaatcat ggagcttttt taacaaacgg ataagccatg
     4681 atgactatat gaaacagcta aatggtttta cgatgagtaa atacgatgct gcggcatggg
     4741 cagacctcat aaaagctagc ggcgccagat atgccgttat cactaccaag catcacgacg
     4801 gtgtagcaat gtacaacacg caaatgaacg atcttagttc attaaaagca acgcctgccg
     4861 ccaaagacat tattaaacct ttttataatg aactccgaca aagggatatc aaatgcggtg
     4921 catatttttc attacttgac tggtcgcatg ataactatcc ggggtttttg cgcgacagtg
     4981 gacgatatga aataaaagat gactgggaac gatggtatcg ttatcgcgat ttttttcaag
     5041 ggcaaataaa agaattactt gctggttata atcctgattt atggtggttt gatggcgatt
     5101 gggagttgag cgccgagcgt tgggaagctg aaaaaattag gcagatgata ctaacgcata
     5161 atccaaatgc aattattaac ggccggcttc aaggttacgg agattatgcc acaccagagc
     5221 aaaactttcc ggtagtacgc ccaaaacata actggtggga actttgtatg actaccaaca
     5281 acagttgggg gttccagttc aaagatcaaa actggaaaac gccttacgaa gtaataacca
     5341 tctttgccga tgtaatatct aatggaggta acctacttct cgatatcgga ccaaaggaag
     5401 atggtacatt tccacctgaa cagataaata ttttaaaaga gttaggccag tggaataaaa
     5461 aacatagcga agcaattttc aattccttgc caggcctgcc tccagggcat ttttacggcc
     5521 caacaacctt gtcgaaagat agtacaaaca tatatctttt ccttgccgga aacccacaga
     5581 atcaaattgt tgtcagaggt ttatttgata atatcgaaaa cgtaagtatt gtaggttcgg
     5641 atatttcatt agaatggaaa atagtcggaa aaatctcttg gagtcttgtg ccaggattgg
     5701 tttatattca ggtgcccgaa aaatatcagg atcaatatat gactgttatc aaaatcaaat
     5761 taaaagacaa agtgcatttg tatcgtggcg aaggtggttt agatatctaa ttaaaataaa
     5821 aaatgaagtt tataagatca gccgttatat tcatttttat cattgtttct ctggagcttt
     5881 ttgctatcgg gtccgacact ctttatttca actctcataa tcgtgaagta gttgttaccg
     5941 atcctactca aggcatcaat tcttatacta agtgggtaaa attcccaagt caagaattag
     6001 acattagaaa aataaccatg tatgtaacct ttggttgccc tgacaccatg cgttgtgccg
     6061 attgggatta ccttgatcat attatggtac agcgagtcgg tggcatcaat tcgagtgatc
     6121 tgaattggga aattggccga atcatcacac cttacggcgg tttttttgac aacgactggc
     6181 agtttaaatg gcaagcagat gtaactgact ttagtatgat tttgcgcgat agcgtggaaa
     6241 ttaattatat acatacgggc tgggagccaa ataatgatcg cggatggtta ataagtattg
     6301 attttgaaat aattacagga actccggtag cagttccgat ttcgattacc gaaatttaca
     6361 acgaccattt cccttacggg gactcgctta agcctattga tcaaacgctt ttaccaaaat
     6421 catttataac tgaaacaaat actgattttg ttagattacg tgtattacag acgggccacg
     6481 gaatggatga gccagataac tgcggagaat tttgcagcaa atatcgggag atataccttg
     6541 acgataatct tgttcaaaaa cgccagatgt ggatgacgtg tggtgataac cctttgtatc
     6601 cacaggccgg cacatggatt ttcgaccgag ctaattggtg tccaggctat ttggttcagc
     6661 cagaaatatt tgatctggca acaacaagtg ggaaggaaca aataatcagg tttattatgg
     6721 aaccttacac tgccacaaag caaaatcagg gaaaacaagt gatcagcgct tacctaattc
     6781 aatatcaaca actccagcac gtactcgaca tttctatcga agacattgta gcgccttcaa
     6841 taaaagatat ttacctgcga aaaaatccag cagcggctaa cccgcaaatt attcttaaaa
     6901 ataatgggaa ggagactata tattccgcaa cgatcatata taaaactgaa ggttttaaag
     6961 agcaaaaaga ggaatggcga ggcgagttac ccgtaggaag ttcagagcta attacccttt
     7021 cgggacttgt caatttctca tcaggtcaaa atatctttac cgcaaaagtt caaaatccta
     7081 atggcaaggc cgaccaatat cctgatgata atgttttatc aacaacattt aactcccctc
     7141 ccgttcatga tactcttttg gtttttagac tattaaccaa taatcagcct gaacacaatt
     7201 catggaaact tatttcacat gatggtaaag tggttgtcga aaagagagct aatgagctga
     7261 aagcccggac tttatactcc gacacgttaa ggctatcaca aggggcttat acgcttttat
     7321 tcagcgatag cgccggcgac gggttagagt tttggtacaa ttccggtgga ggacgtggag
     7381 aggcatggct attagatggg aacgacaatc tgataaaagc ttttgaacca gattgtggat
     7441 tagggtggat atataatttt acggttagcc aaaaccctga tcctatcaac agacagaaca
     7501 aagcaataag cctataccct gcccgcacct ctgattttac taaacttaca tattttgcca
     7561 accatcaaaa agatatcatc gtaaaactaa ttagcgatcc aggtggagaa gtagtggaag
     7621 aaagaaatta tccgaatttg aaaaagggaa tttttgattt tgatcttaca agattttcat
     7681 acggtcgctt cttccttaaa gtatttgcag atgggcaaga gatttttaac aagcgaatcc
     7741 gatatgtgga gccggcaaaa gagcccgatg ccgatttcta tgtctggcct accgacccat
     7801 tggtaagcca aaagctacac gattggcagg attggaagtt tggcgtcata atacattggg
     7861 ggccatatag cgaatggggt gttgtagaaa gctggagcct gtgcccggaa gatgagcctt
     7921 ggtgcattag acaaggacct tttgcacacg attattatac ctacgttcag gagtatgaaa
     7981 aaatccaaca aacatttaat cctcaaaagt ttaatcctga caaatgggct gacgcttgca
     8041 aacgggcagg gatgaaatat gtgattttca ctactaaaca tcatgatggt ttttgtatgt
     8101 acgactcaaa gtacaccgat tataaaatta ccggttcaga gagccttttt gcccaaaatc
     8161 ctaagagtaa tatagctttt gaagtgtttg aagcatttag aaagacagga atgagaacag
     8221 gcgcttattt tagcaaacct gactggcata atgacgacta ctggtggcct tacttcccgg
     8281 ttttcgacag gaatgtaaat tatgcccctt ctaaatatcc tgaacgatgg gagcgttttc
     8341 ggaaatttgt ttttaatcag attgaagagc tgatgggtaa ttatggtaaa attgatatcc
     8401 tatggcttga tggcggttgg gtgagacctg tcaaaaccgc ctcaaatgaa tcaaatcaag
     8461 agataataga gaaaggttat aatcaggata ttgacatgcc atctattgca aaaagagcgc
     8521 gtcagttgca gcccgacctt ataatcgttg accggacagt gcatggcatg tacgaaaatt
     8581 atactactcc cgaacaagaa atacccgatc atgttccgaa tcatccgtgg gaaagttgta
     8641 taaccctcgg tgatagctgg tatcacacag gcccggagga gaattataaa tctttaacat
     8701 gggcggtgca tacacttata aagattattg caaaaggcgg caacctgtta cttggtatcg
     8761 gccccgacaa aactggtgat tttgcaccgg aagtttacac ccgtctcgaa gaaatcggga
     8821 actggatcaa tgttaacaat gaagcgattt acgggacaaa accgcttgcc ccctatcatc
     8881 atggtaattt atattttact caagcaaaag atgaaaaaac caaatttgct ttctgcctta
     8941 aagaggagtc agataatttt ccgaacgaaa taaaactgcc gttcggcgta gatattcgca
     9001 aggcaaaaat taaactactc ggttacaaag gtagcatcag agccacatct attcaagacg
     9061 aaaccataat caaacttcca aaactaacga ccaaattaaa aaactcacct gcattagtgt
     9121 tcagtatcag cattacaaat cagtattaag catttgataa atgattaggg tatgttgaat
     9181 aagttataag agctttgcga gatttttctc gaaaaatttc tttcaataat tctctttttt
     9241 tgctttttgc cagagtgtta tatctttgtt tcgtaaatat ggatgttata gagcattaaa
     9301 aaaagataat ttgtatataa aaagggttaa aaaatgatag agaaattatc aattattatt
     9361 ccagcctaca atgaaggcag aactattcac ctaatactta ataaagtaaa agaggtgaat
     9421 ttaattaaca atattacgaa agaagtaatt attgttaacg attgttcaac agatgagacc
     9481 gaaagtataa ttgagaatta cattgaagct aacccatctt ttaatattca gtatttcaag
     9541 catgatataa atatgggtaa aggtgcagca ttgcatactg gaattagcaa agctactgga
     9601 caatacctaa ttattcaaga tgcagactta gagtatgacc cgcaagaata caacgactta
     9661 ttaaaaccag ttgtgaatgg atttgctgat gttgtttatg gctctcgatt cataggggga
     9721 aatccacatc ggatattatt ttactggcat acaataggca ataaatttct tactgccttg
     9781 tcaaatatgt tcaccaactt aaacttgacg gatatggaaa cttgctataa attatttaat
     9841 actaagttaa tccagtcaat acacttaaaa gaaaaacgtt ttggctttga gccagaagta
     9901 acagcaaaaa tcgcaagaat taaaggaatt agaatttatg aagtcgggat ttcctattat
     9961 ggaagaacct acaaagaagg taaaaaagtt ggttggaaag acggctttcg tgcattatat
    10021 tcaataataa aatatgcgct tttaaaaaaa tgatattata aaaaaacggt agtatctcat
    10081 aaactacgta attttatgag atactaccaa atttattttt tttgattggc aaagggcata
    10141 aaaataacct gctccatcct gtttttgaac aattctgcaa aaaaacattt ttgaacttaa
    10201 taaggttctt cgatattaat tctgattggt tgactattga tttctcacca aatcctaatt
    10261 agttcgaata aacattttcc tttttttata aataaaaagc taatggttta agaagaaata
    10321 atatttttac agccaatttt aaaatttaat agatgaacaa gtcatttctt ttattagcca
    10381 cctttctatc cttttttttg ttatcgcaaa atattattgc acaggatatt acaccgaata
    10441 aaaagatgta tggtaagtca ttttaccatc aaaaagctcc tgagtttgta gttgaaaaat
    10501 ggctgtctga cgtacctgat atgaagggaa agttcattat gattgatttt tgggctacct
    10561 ggtgcggacc atgtaaaaga tctattccac agataaacga gtggtataat aaatacaaag
    10621 atcgaatggt tgttattggc gtatctgacg agccggaagc aaaagtgtta gcgctgaagg
    10681 agcctaaaaa agaatattat agcgccattg acaccaaaaa gagaatgagc aaaacatatg
    10741 aagtatcagg aattcctcat atagtaataa ttgatccaca aggtattgtt cggtggcaag
    10801 gttttccgtt tcttgcaggt tacgaactaa ccgatgaagt gattgacgag atattggaaa
    10861 aatatagtaa ataagcgatt gctattgtct gtggctttat ctatctcctt ctttagcttt
    10921 atttgaactt tgtctttaca gtggttttta cctattaggc atcagtacat attctaaggc
    10981 ttattttgaa tgatgagttt ttttttaagt caaatagctt ataacagatt tctgaaatac
    11041 tcgatggttt tcattaatcc ctcctcaagc ttaattttag gttcccatcc aagtaattgt
    11101 cgggctttag agatatccgg ttttcgctgc aaaggatcat ctgaaggcaa agggtgatat
    11161 acgattttgg attttgatcc ggtcattcgt actactgtct cggcaagctg atgtattgta
    11221 aactcttcag gatttcctat atttatcggc cctgtaattt tatcgtcagt atccatcata
    11281 tttatcattc ctgttaccat atcgtccaca tagcagaaac tgcgggtttg ctggccattt
    11341 ccataaatcg tgatctcttt gttttgtagg gcctgcatta taaaattcga cacaactctt
    11401 ccatcgttag ggtgcattcg tgggccgtaa gtattaaaaa ttctaatgat cttaatcctg
    11461 actccatttt gtttgtgata attcacaaat aaggtttctg ctgctcgttt gccttcgtca
    11521 taacatgccc tgtctcctat cggattaacg tttccccagt attcttcgtg ctgcgggtga
    11581 atctcaggat cgccatacac ctcgctcgtt gaggcctgga gaacctttgc acggattcgt
    11641 ttagctaatc caagcatatt aatagcgccc atgaccgagg ttttaaccgt tttaatagga
    11701 ttatactggt aatgaatcgg tgaggcaggg caagcaaggt tataaatctc gtcaacctcg
    11761 acataatatg gcatagttac atcatgccga acgagttcaa aatagggatg accaataaga
    11821 tgaactacat tttgcttttg tcctgtaaaa taattatcca aacatattac gtcattgcct
    11881 tctgatagta atcgttcgca caggtgcgat ccaagaaagc cagcgccacc ggtaacaagt
    11941 attcgttttt tcattattag tcaggattaa gtgttaatga gaatttacat tagcgccgat
    12001 acaataatat tcaaaaccgg cttttttaac ctcattacga tcaaagatgt tacggccgtc
    12061 aaagattaca tgttttgcca ttaacttttg cataagcttg tattttggaa agcggaattc
    12121 gatccattca gtaacaatga gcaaagcatc aacatttgtt atggcatcat agggatcgtt
    12181 agcatattcg atactatctc caaaaatttg gcgtacattt tccatagcta ccggatcgta
    12241 tgcttttact ttacaacctg cttttaaaag cctttcgatg ataaccagtg acggcgcttc
    12301 acgtatatca tcggtttgcg gcttaaaaga aagcccccat attccaacgg ataacccttt
    12361 aagattgcca tcaaaataat caagcagctt tttaaaaagc gccaccttct gatcctcatt
    12421 aacatcttca accgattgaa tgatacgaag tttataatta tactcttcac cggtttttat
    12481 caacgcttta acgtcttttg gaaagcatga gcctccataa ccaattcccg ggtaaaggaa
    12541 tttactacct attctcgaat cgcttccaat tcctctccgt accatattca cattagctcc
    12601 caataaatcg cacaggtttg caatatcgtt cataaagctg atacgggtgg caagcatagc
    12661 attagcagca tatttagtca tttcggcgga tggcacatcc ataaaaagca cagggtgatt
    12721 attcaatgta aatggacggt agagcctcga catcacccct tttgcaaatt cgctctctac
    12781 accgatcaca atcctgtcgg gcttcataaa gtcgtcaata gcggcgccct ctttaagaaa
    12841 ctcaggattt gaaacaacgt cgaagtttat ttttaatcct cgtttatcta attcttcctg
    12901 aattgtattt cttaccttct tagatgttcc aaccggaacg gtgctcttta ccacaaagac
    12961 caaatgtttt ttcatactcc tgcccactgt tttggcaaca ttgagcacat attttaaatc
    13021 tgcgcttcca tcttcatcag gaggtgtgcc cactgccgaa aatattattt cggattcgtt
    13081 taccaaaggg gtgagatcgg tagaaaaaga gagttttcca cgttctatat tacgcttcaa
    13141 catatcatcc aaacccggtt cgtaaatcgg cgatattcct ttttttaatt tttcaatttt
    13201 tgctatgtca atatccacac aagttacatc aatacctaat tcagcaaagc aagttccggt
    13261 aaccaaacca acataacccg aaccgacaat agtaattctc atgttttaac aattaaaaaa
    13321 ttaataattt agcaacctaa cgtattaatc tgatcgaata atacacttta attatctttt
    13381 tcaaacttag ttttattcaa atgtccaaaa tccagtctga tttcaaaata gacgcgatta
    13441 tttgcacgtt taaattccaa attataaaaa aagcctggct attaaaatta ttccaggctc
    13501 tttttagtgt ttcaaaatat ttattgcaat actacctttt taatgtaagc gccatcgcta
    13561 ctatttatgc gcaggtaata aacgcctctg gcaaggttat caagtctaat ctcttggctc
    13621 actgatccat ttaacttccc gatataatta ctataaactt gttgcccatg ataattaagc
    13681 aatgcatatt caatttcatc agtaacgcct gtaaatgaga tattcagcag atttttagcc
    13741 ggatttggat agacaagcat actaatatta tcgctaattt catttattcc aacacatgat
    13801 ttaaaggtta tggtaatatc atcctgtcca acacaaccgt aaacatcagt aacctgacac
    13861 gtatatgtaa cagatgcgat tcccacaccg gttgtatcaa cttgaataac tgcagtagtg
    13921 tatcctccgg gtgtccaaag atagctaacg gcattaggtg tagtggcgtc gagtgttacg
    13981 gtttcggttt cgcaaacggt aacatcctca ccaaggttga ctggcggaag ttcatgaact
    14041 actaatgtat aattatccga aattgtggtt gctccatcgt taattgttac agtatatgtg
    14101 gttgtttcat ttggtgtagc aatggggttt tgaacattcg ggttgctaag ggatgaagcc
    14161 ggcgaccagt taaagttgta gtttccggta ccaccgctga cattacaatt aagtagcgaa
    14221 taaccaccct gacaaatttc ttctgggttt gcagtgatat tacaagccaa gttacccact
    14281 gttataaagt tggctatcca tccagattta acaactgaat agtcggaagt aaatctgaat
    14341 gtaatcgctc cagtcggatg agttgaagta attgttccag gactataagt accggtgaat
    14401 gggcttcctg ggaattgtgt ggcggtggta ttggggccat tgtaaatata aagatagtcc
    14461 caattattct ctatatcaaa ctgagtaaag gtaaatttaa gcggtgtgtt ttcatcaata
    14521 ggtagaaatg tcatggtgta attttcgtgg tttgaatagt tggcattcgg gccgccagaa
    14581 tcatagaaaa cgccactgtt taccgtaacg gtaccattag acatataata ttcaggtccg
    14641 ctggtagtaa atgaccatac ggggcaatca cttgcctgac catttgcatt tattgggatt
    14701 attttccagt aataggttgt gtttggttct aacacaggtg tatataaagg tgttggcacc
    14761 gtttcaacaa agggtggatc aggtacaagg ccaaaatata catcaaacgt tactgcatca
    14821 taagataccc atgacaggtt agaattcaaa gaaacattct gagcattatt ggttggactt
    14881 ggagatgaag cacactcagg aagcccttgg agataatgac aactaatgtg agcgctccaa
    14941 ccggctt
//
LOCUS       DAOOAI010000053        14742 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_5272_length_14742_cov_4.574454, whole genome shotgun sequence.
ACCESSION   DAOOAI010000053 DAOOAI010000000
VERSION     DAOOAI010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14742)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14742)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14742
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5272_length_14742_cov_4.574454"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..593
                     /locus_tag="PK345_05240"
     CDS             <1..593
                     /locus_tag="PK345_05240"
                     /inference="COORDINATES: protein motif:HMM:NF019339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX34454.1"
                     /translation="YKEPAIYRFLAEIYKSESDTTQALKCIHDGRSKFPDDFGLIIEE
                     TNIYLASNEKEKAMELLQLAITKDDKNPTLFFAVGANYDQMGDFDEAEKNYKKSLELD
                     PEFFDSNYNLGALYVNKAITITEEANALPLNEEKKYNELKEQSDELLEKSLPYLEKAD
                     ELNPNDIYVLRTLRDIYARLNQLDKLREIEKKIDKD"
     gene            complement(754..3744)
                     /locus_tag="PK345_05245"
     CDS             complement(754..3744)
                     /locus_tag="PK345_05245"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPX34455.1"
                     /translation="MKFKYLIAFLITLFFTNLSNSQVVINEYSVSNLSQFPDNYQKYE
                     DWFELYNTSTTSINIGGYYLSDRPGNPFKWQIPENTVISGNGFLRFWASGRNEASGNH
                     YHTNFRLTQTKNNPDYVILSNPFGTAIDSQQLQITQLGHSRGRFPNGSDNWVIFTNPT
                     PNTSNNSAQAYIGYAEKPEMSHQAGFYFNGQSVTISTNEPEVTIRYTTNGSTPTSNSA
                     IYTNPINIDNTTLLQAKAFHSNPDILPSLVEFNTYFINESSTLPVLSISGDQLTQLLN
                     GNASLRPKGTIEYFNANKVRTAIATGEFNEHGQDSWVHPQRSIDLITRDEMGHNYAVK
                     EKLLTISERDEFQRIILRAAGDDNYPGIDSSAHMRDLWAQNMAEISGMSLDVRKGARI
                     LLFANGQFWGVYSIREKVSDHDFTKFYYNQDKYDLQFLMYWGGLWAQYGGQQAINDWL
                     DLRTYILNNDMTNPFAFFYVDQLYDYKSLVDYVIINSFVVCSDWLNWNVGWWRGLNPD
                     GTHQKWGYILWDEDAILGHYVNYTGIPGQHPYVSPCYPEYLSSYSDPQKHVEILKKLM
                     YNPDFYQYYVSRYIDLLNTAFVKERLIASIDSIADVFRPEMPKHIERWGGNMTKWENN
                     VQKLRNFISNRVDYVPSGLKSCYDLTGPFPITVNVEPAGSGIIRLNSLELGVFPWDAN
                     YFGNIDIKLEALTSKVEFEFDYWEISNHTPTPDTSSTTITLNLINNADITAHFKLKTY
                     TDSLIINEINYKSATWFNTEDWIEFYNPHNYDLDISNWLFKDGNDNNSYFFPPNTIIA
                     ANGYLVLCKDTTAFKSLQPLVTNYLGNMDFGFSANGELLRLYNSEGLLIDTVHYGNDD
                     PWPPEANGLGPTLELIKPSLDNAQPENWMASAANGTPGAMNSLIAEIKEKKNLKEKFA
                     ISVFPNPANENVYIWIHEENLIKNGLIQIYNSMGRKVFHKENIRSDKHEINVMDFPDG
                     LYIVKFYDKGTELSASSKLIINRK"
     gene            3927..4388
                     /gene="coaD"
                     /locus_tag="PK345_05250"
     CDS             3927..4388
                     /gene="coaD"
                     /locus_tag="PK345_05250"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HPX34456.1"
                     /translation="MAGIAVFPGSFDPITKGHEDIIKRAIPLFDKIIVAVGQNQDKQT
                     FFPLEKRIEFLKFVFRDYPSVEIDQYSGLTVDYCKSKGAQYILRGLRTSADFEFERSI
                     AQVNKKLDHELETIFLLTLPEYTALNSSVVRDILRNGGNPAQFVPEGLILK"
     gene            4585..7887
                     /gene="secA"
                     /locus_tag="PK345_05255"
     CDS             4585..7887
                     /gene="secA"
                     /locus_tag="PK345_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HPX34457.1"
                     /translation="MIKNILKSFFGTKADKDIKEITPVLNDILKAYETIKNLSNDELR
                     GKTISFRKNIAEYIAEEENKIQKIKQQLENEPDLDADEKEKLYDEMDRHEKVSYDKSQ
                     EILNKILPEAFAVIKETARRFKENDEVIVTATDMDRDLAASLDNVNIVGDKAHYKNQW
                     MAGGNMIKWDMVHYDVQLIGGIILHQGKIAEMATGEGKTLVATLPLYLNALPGKGVHL
                     VTVNDYLAKRDSEWMGMIYMFHGLKVDCIDKHEPNSEARRAAYMADITYGTNNEFGFD
                     YLRDNMTGNPDELVQRPHNYSIVDEVDSVLIDDARTPLIISGPTAKGENQLFDEMKEN
                     VQRLYNSQRNLVTKLLAEAKQILGGDNVSDDDQKKGGEYLLRAHHGLPKNNALIKFLS
                     EPGMKSLMTKTESFYMQEQSKHMHIINDPLYFVIDEKNNSIELTDNGIDLLSSSYNDP
                     SFFIIPDMGSELADLDKSDLSEAKKTEKRNEIMRDFSIKSERIHTVNQLLRAYSMFEK
                     DVEYVVMDNKVKIVDEQTGRILEGRRYSDGLHQAIEAKENVKIEAATQTYATITLQNY
                     FRMYKKLAGMTGTAETEAGELWDIYKLDVVVIPTNRPIVRDDREDLVYKTKREKFNAV
                     IEDILNLVEKDRPVLVGTTSVETSELLSRMLTRQKIPHNVLNAKLHQREADIVAEAGK
                     PGKVTIATNMAGRGTDIKLGAGVKEAGGLAIIGTERHESRRVDRQLRGRSGRQGDPGS
                     SQFFVSLEDDLMRMFGSERISKIMDRMGLKEGEVIQHPMITKSIERAQRKVEENNFGI
                     RKRLLEYDDVMNQQREVIYRRRRNALFGERLEVDISNMMYDLCDQVISDYQDTGDFEE
                     FRLEVLRDMAITVPFENKDFLQESVDTLAEKLYNKVYQSYKLKMKAIAEKAAPVIKDV
                     FENHTNFENIAIPISDGIKTRNIIVNLKKAYENGAREINLAIEKSVTLGMIDEAWKEH
                     LREMDDLKQSVQAATYEQKDPLLIYKFESFQLFKRMVQRINREITSFLIKGDLPIQSS
                     GDVREAQLPRRTDRRRMSESRDDLLSQAHSNTQQRQKPEPVKVEKKVGRNEPCPCGSG
                     KKYKHCHGRAGG"
     gene            8175..9824
                     /locus_tag="PK345_05260"
     CDS             8175..9824
                     /locus_tag="PK345_05260"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF006821.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HPX34458.1"
                     /translation="MNNTLREKLTKVIQQHGNIFNNIPRREIIRMVVENREAIIAKSG
                     ALATWTKTESNGRSPKDTVSVKRSSSEHNIDWTSPNNIPIDEETFDMLFDDALQFIRD
                     AKQVQITDRVIGADSKYALPVRVVTPNALHALFTDNMFRPIPKDIHRSKFYQNGFELI
                     AVPYRKLNPDKYEGRLRKLPNGKTTDMAVAMDFDRRLGVIIGSAYMGSVKKMLFTVMN
                     YLLPLEGILPLHCSANEGPKGDSALLLGLSGTGKTTLSADPSRALLGDDEHGWSDDGI
                     ANFEFGCYAKMIDINPKKEPDIYDAVMHVDDYLNHGSIVENAMIYPDGTFDFYDDRLT
                     PNSRASFLLTYLKNIKESSTSGHPKTILFLTADAYGVLPPVSRLTEDEAMLWFLMGYT
                     SKLAGTETGVTEPQATFSRFFGQPFMPANPDVYAEMLGKKMKEHNTKVYLINTGWSGG
                     AYGTGQRIDLTLTRKMVNAALSGELENVNYKRDNLFHVDVPENCPGVPSEMLWPKNTW
                     KDKEAYDKQAQILAKKFSDAFDKNYGQKNIKESVIKVCPGK"
     gene            9905..10570
                     /locus_tag="PK345_05265"
     CDS             9905..10570
                     /locus_tag="PK345_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002559825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HPX34459.1"
                     /translation="MGINQNIKKIKNELPANVKLIAVSKTKGIDEIMEAYNAGQRHFG
                     ENKVQEMIAKQPHLPSDIHWHFIGHLQTNKVKFIAPFVNMIESVDRLKVLKEINKHAA
                     ALNRVIPCLLQFHIAEEESKFGLTLSESYEILDNNYFDKLKNVSICGVMGMATLTDDK
                     TQISKEFKNLKIIYDILKQKYFQHIDSFKEISMGMSGDYLIAIEQGSTIIRLGSVIFG
                     QRD"
     gene            10994..13270
                     /locus_tag="PK345_05270"
     CDS             10994..13270
                     /locus_tag="PK345_05270"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HPX34460.1"
                     /translation="MDIYLIIIIILIAFAVADLAVGVTNDAVNFLNSAAGSKTAPMWV
                     LLIVVSFGVILGATFSSGMMEIAQKGIFHPGQFTFSEVMIIFLAVGMADIILLDLFNT
                     FGLPTSTTVSIIFELLGASVAISLIKMNISGESIVELGNYINSPKVLAIISAILISVF
                     LGFIIGAIIQYVVRLIFTFNFVPKLKYYGGVFGGLSITAITFFMILKGAKGASFLTPE
                     MNEWLESHTIHILLYGFIFWTVFLQLLYWIFKVPITKIVVIIGTFALAFAFAGNDLVN
                     FIGVPLAGLASYKSFLSTPGATPDTFYMDSLSRAVTTDTIYLVIAGVIMALTLWFSKK
                     ARTVIATEVNLARQGSGYERFGTSALSKSVVRGVIKLNKSFSEKIPKRIQNRIDKRFE
                     IPKTIYSSVKTKDLPSFDELRASVNLMVSSVLIALGTSLKLPLSTTYITFMVAMGTSL
                     SDRAWGRDSAVYRVQGVFSVIGGWFLTAVLAFFIALIIAIVIYFGGIVAIGVIVILVL
                     LFVYRTYKLHRTMEKERTDNAIEHEIEEIISASVVFEKCISLTVNALEKVPDFYRQLF
                     KGITEEDRNSLKELKKEIKKFNKKTKRIKDNVSETVTHLQIDQIDTGHYYVQSVDYLR
                     EIAHCLNYMANPAYEHIDNNHTGLDEFQQQEFEAIYSGISELYKDIIVKIQTKKYSDL
                     DQIILKQQEILQLISESRINQVKRLKEKQLHTRVSLLYFDLLAETKNLLLYSINLLKS
                     QRDFRSEILRSQPGFYSG"
     gene            complement(13298..14173)
                     /gene="prmC"
                     /locus_tag="PK345_05275"
     CDS             complement(13298..14173)
                     /gene="prmC"
                     /locus_tag="PK345_05275"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HPX34461.1"
                     /translation="MDFRSNKVKEIRETYIKELQSYYPVHEATALLDLILSHRLLINR
                     TTLALNPELRVSESEMLIVHFDVKELKKYRPIQYIFGEAWFDGFSLKVNENVLIPRPE
                     TEELVLWAVDSLQNKKNLSILDIGTGSGCIAIALAKRLPTSHISACDVSRDALILAGE
                     NAKNLKLNIDFFNLDILKSDSWINACVYDVIISNPPYVTNSEKAAMQPNVLNWEPSLA
                     LFVNDDDPLIYYRKTLQFCINHLKSSGTIFFEINQHFGNELYDLLSSSGFKDIIIKKD
                     LSGNDRMLSAVKLQQ"
     gene            14469..>14742
                     /locus_tag="PK345_05280"
     CDS             14469..>14742
                     /locus_tag="PK345_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34462.1"
                     /translation="MRIGLLIILFFIPPLLISAQPEKYTRIEIPCSLENIEEVYRLGI
                     NIAFIEPDDYLWLEVSEGEFALLKTTGIDYRILIEDISEFYRSRNSN"
BASE COUNT         4783 a         2524 c         2889 g         4546 t
ORIGIN      
        1 attataaaga acccgctatt tatcgtttct tagcagagat ctacaaatcg gaaagtgata
       61 caactcaagc tcttaaatgt attcatgatg gcagaagtaa atttcctgat gatttcggat
      121 tgataatcga agagacgaat atttaccttg catcaaacga gaaagaaaaa gccatggaac
      181 ttctacagct tgccattaca aaagatgaca aaaatccaac tcttttcttt gccgttggtg
      241 ctaattatga ccagatgggc gactttgatg aagcagagaa aaattataaa aagtcattag
      301 agctcgatcc tgagtttttt gattcaaact acaacctcgg agcgctttat gttaataaag
      361 ctatcacgat taccgaagag gcaaatgctt tacctcttaa tgaggaaaag aaatataatg
      421 aattaaaaga acagtcagat gaattgctcg aaaaatcttt gccgtatctt gaaaaagctg
      481 atgagttaaa tccgaacgat atttatgtac taagaacttt gagagatatt tatgcccgac
      541 ttaatcaatt ggataaactg agagaaattg agaaaaaaat agataaagac tgattttatt
      601 gcgaagggga ggaatcggta gcggttcctt cacttttttc atttgtattt aacataatat
      661 aaattataag acatattata tttttctttg tgggaacaaa taggtttttt gaacgagaaa
      721 gtttacgaat ttgacaaagg aatgttttaa gtattatttt cgattgataa ttaattttga
      781 tgatgcgctt aattctgttc ctttatcata aaacttaacg atatacaagc catcaggaaa
      841 gtccatgaca tttatttcat gtttatcaga tcttatgttt tctttatgaa aaacttttcg
      901 ccccattgaa ttgtatattt gaatcaatcc attttttatc aggttttcct catgaatcca
      961 gatgtaaaca ttttcgtttg ctggattagg aaaaactgaa attgcaaact tttcttttaa
     1021 atttttcttt tcctttattt ctgcgattaa gctgttcatc gccccaggtg tgccgttagc
     1081 ggctgacgcc atccagtttt ccggctgagc gttgtctaat gatggtttaa ttagctctaa
     1141 tgtaggtcca agaccattag cctctggcgg ccatggatca tcgttgccat aatgcacagt
     1201 atcaataagt aagccttctg aattataaag tctaagcaat tctccgtttg ctgaaaaacc
     1261 aaagtccata ttacctaaat aatttgtaac taagggctgt aatgatttaa aggcggtggt
     1321 atccttacac agtaccaaat atccatttgc tgctataatg gtatttggcg gaaaaaaata
     1381 agaattgttg tcatttccat ctttaaaaag ccagtttgaa atatctaggt cataattatg
     1441 tgggttgtaa aattcaatcc agtcttcggt attgaaccat gttgctgatt tataatttat
     1501 ctcattaata atcaatgagt cagtataggt ttttaattta aaatgcgctg ttatatccgc
     1561 attattaatc aaattcaatg taattgttgt tgaggaagtg tcaggggttg gagtgtgatt
     1621 agatatttcc caataatcga attcaaattc aacttttgat gtgagcgctt ccaactttat
     1681 atcaatattc ccaaaataat ttgcatccca tggaaacact cctaattcga gtgaattaag
     1741 acggataatt ccactacctg ccggttcgac attcactgta attggaaatg gtccggtaag
     1801 gtcgtaacag cttttgaggc cggaaggtac ataatctaca cggtttgaga taaaatttct
     1861 caatttctga acattatttt cccattttgt catattacca ccccatcttt caatatgttt
     1921 gggcatctcg ggtctaaaaa catcagcgat gctatcaatg gaggcaataa gtctttcttt
     1981 aacaaatgct gtgttaagta aatcaatgta tcttgacacg taatattgat aaaaatcagg
     2041 attgtacatc aactttttta agatttcaac atgtttttga ggatcagaat agctggataa
     2101 atattccgga taacacggtg atacatatgg atgctgtcct ggtatgccag tataatttac
     2161 gtaatgcccc aaaattgcat cttcatccca aagtatatat ccccactttt gatgagttcc
     2221 atcagggttt aaacctctcc accaccccac attccagttg agccagtcgg agcaaaccac
     2281 aaatgagttg ataataacgt aatcaactaa agacttataa tcataaagtt ggtcaacata
     2341 aaaaaatgca aaaggattcg tcatgtcatt gttcaggatg taggttctca gatccaacca
     2401 gtcgttaatg gcttgttgcc cgccgtattg tgcccatagt cctccccaat acattagaaa
     2461 ttgcagatcg tatttatcct ggttataata aaatttcgta aaatcatggt cgcttacctt
     2521 ttcccgaatt gaatatactc cccaaaattg cccgttagca aaaagcaaaa ttctggcgcc
     2581 ttttctcacg tcgagactca tgcccgatat ttctgccatg ttttgagccc atagatcacg
     2641 catgtgcgca gatgaatcaa tgcccggata attatcatcg ccggcggcgc gtaatataat
     2701 acgttgaaat tcatcacgct cggatatggt aagtaatttt tcctttacag cgtaattatg
     2761 tcccatttcg tcacgggtaa ttaagtcaat actacgttgt ggatgcaccc atgaatcctg
     2821 accatgttca ttaaactcgc cagtagcaat tgctgtacgt actttgttcg cattgaaata
     2881 ttcaatcgtt ccttttggcc ttaggcttgc attaccattc aataattggg tcaattgatc
     2941 acctgatata gacaataccg gtagcgtgct gctctcatta ataaaataag tgttgaattc
     3001 aactaaactt ggtaatatat cgggattaga atgaaaagct ttggcttgta acaaagttgt
     3061 attatcaata ttaatgggat tggtgtaaat agctgaattt gaggtaggtg tggatccgtt
     3121 agttgtatat ctaattgtaa cttcaggttc gttagtggaa atagtaactg attgtccatt
     3181 aaaatagaaa ccggcttgat ggctcatttc gggcttttca gcataaccaa tataagcttg
     3241 tgccgagttg ttgcttgtat ttggtgttgg attggtaaag attacccagt tgtcagaacc
     3301 attcggaaac ctaccacgcg aatggccaag ttgtgttatt tgaagttgtt gggaatcaat
     3361 agctgtaccg aacgggttag agagaattac ataatcagga ttgttctttg tttgagtaag
     3421 gcgaaaatta gtgtggtaat gatttcctga tgcctcattt ctgcctgatg cccaaaatct
     3481 taaaaaccca tttcctgata ttacagtatt ttcaggaatt tgccatttaa aaggattacc
     3541 cgggcgatcg cttaaataat aacctccaat atttatggat gtagtactag tattgtataa
     3601 ttcaaaccaa tcttcatatt tctgataatt atctggaaat tgacttaagt tagagacaga
     3661 gtactcgttt atcacaacct gagaattaga aaggtttgta aagaaaagag tgataagaaa
     3721 tgcgatcaga tacttaaatt tcatgctaca tagtgttttt gttgtcataa atagttgttt
     3781 ttattacaag agaaaatacc ctctcccttt aaaaactttc caaaaatagt aattttaaag
     3841 ttttaccatt gaagctaaga ggcataattt ctgtcaatta tctttttctt atgaatttca
     3901 attacctttg ctcaaaaata aatgttatgg ctggtatagc agtttttccc ggctcgtttg
     3961 atcctattac aaaaggacat gaagatatca ttaagagagc aattcctctt tttgataaaa
     4021 tcattgtagc cgtaggccaa aatcaggata aacagacgtt ttttccatta gaaaaacgga
     4081 ttgagttttt aaagtttgtt tttagagatt atccaagtgt ggaaattgac caatatagcg
     4141 gtttaactgt agattattgt aaaagtaaag gagctcaata tattttgaga ggtttacgta
     4201 cttcggccga ttttgagttt gaacgttcaa tagctcaagt taacaagaaa ctagatcatg
     4261 aattggaaac catctttctg ttgacacttc cagaatatac tgcactgaac tcatctgtgg
     4321 taagggacat tttgcgaaat ggcggcaatc ctgcacaatt tgtacctgaa ggactaatac
     4381 tcaagtgaaa gccttgaaaa atattgataa tgagaaagtt aaatctttta taaaaaggct
     4441 tgaactcttt ttttattcct cagtcaatat tttgaattaa ctttgcagat gcctgattta
     4501 ggccataatg gcataaaaaa atattgtttt tttaaggcat tattgttgat gattgggctt
     4561 ttttttgctt gaaaaatcat aatcatgata aaaaatattc taaaaagctt ttttggtact
     4621 aaagccgata aagacattaa agagattacc ccagttttaa acgatattct caaagcttat
     4681 gaaaccataa aaaacctttc gaatgatgaa ttgaggggaa aaactatttc gtttaggaaa
     4741 aatattgcag aatatattgc tgaggaggaa aataaaattc agaaaatcaa acaacagctt
     4801 gaaaatgagc ctgatcttga tgctgatgag aaggagaaat tgtatgatga aatggataga
     4861 cacgaaaagg ttagttatga caaatcgcaa gagattctca ataaaatttt acctgaagca
     4921 tttgctgtaa tcaaagagac agcccgtcgt ttcaaggaga atgacgaggt tatagttaca
     4981 gcaacggata tggatcgcga tctggctgca agccttgata atgtgaatat cgttggcgac
     5041 aaggctcatt ataaaaacca gtggatggcc ggtggaaata tgataaaatg ggacatggtg
     5101 cactatgatg ttcaacttat cggcggtata atattacatc agggtaagat agccgaaatg
     5161 gctaccggcg aaggtaaaac acttgtagct accctcccac tctacctcaa cgctttgccg
     5221 ggaaaaggtg ttcatttggt tactgtaaac gactatctgg ctaagcgcga ctctgaatgg
     5281 atggggatga tctatatgtt tcacggacta aaggttgact gtattgataa acatgaacca
     5341 aactcagagg ctcgtcgagc agcttacatg gcagatatca cctatggaac caataatgaa
     5401 tttggtttcg attaccttcg tgacaatatg acgggaaacc ctgatgaatt agtccaacgc
     5461 ccacataatt actcaattgt ggatgaggtg gattctgtgt tgattgacga tgctcgtacg
     5521 ccgcttatca tatcggggcc aacagcaaag ggcgaaaatc agctttttga tgaaatgaaa
     5581 gagaacgtcc agcgactcta taattcgcaa agaaatcttg ttacaaaatt attggcagaa
     5641 gccaaacaaa ttcttggtgg cgataatgtg agtgatgatg atcaaaaaaa gggaggcgaa
     5701 tatttgctgc gagcacatca cgggctgcca aaaaacaatg cgctgattaa atttcttagc
     5761 gagcccggaa tgaaatcatt gatgacaaaa accgaaagct tttacatgca ggaacagagc
     5821 aaacatatgc atattattaa tgatcccctt tattttgtca ttgatgaaaa aaataattct
     5881 attgaattga cagataatgg aatagatcta cttagctcat cctataacga tccatcattc
     5941 ttcataattc ctgatatggg ctccgaattg gccgatttgg ataaatcaga tctgtcagaa
     6001 gccaaaaaaa cggaaaagcg taacgaaatc atgcgcgact tttcaattaa atcagaaaga
     6061 attcatactg taaatcaact cctacgggca tactctatgt ttgaaaaaga tgtggagtat
     6121 gtggtgatgg acaataaagt aaagatcgtg gatgagcaaa caggtcgtat tcttgaagga
     6181 cggcgatatt ctgatggatt gcatcaggca attgaggcta aagagaatgt aaagattgag
     6241 gcagctacac aaacttatgc caccattacc ctgcaaaatt acttcaggat gtataaaaag
     6301 ttagcaggaa tgaccggaac tgccgaaacg gaagcgggag agttatggga tatttataaa
     6361 cttgatgttg ttgtaattcc tacaaatcgc cctatagtgc gtgatgatcg cgaagatttg
     6421 gtttacaaaa ccaaaagaga aaaatttaac gcagtaatag aggatatttt aaatctcgta
     6481 gaaaaagatc ggccggtgtt agttggaaca acttcggttg agacctctga gctgcttagc
     6541 cgaatgttaa cgcggcaaaa aatccctcat aatgtgttaa atgcgaaatt gcaccaacgc
     6601 gaagctgata ttgtggcaga agcgggaaaa cccggaaagg ttacgattgc tacaaacatg
     6661 gctggccgcg gaaccgatat taagctcggg gctggagtga aggaggccgg tggtcttgct
     6721 attatcggaa cagaacgaca tgaatctcgg cgcgttgacc ggcagctaag aggacgatcg
     6781 ggacgacagg gagaccccgg tagctcgcaa tttttcgttt cacttgaaga tgacttgatg
     6841 cgtatgttcg gatcggaacg catttcaaaa ataatggaca ggatggggtt aaaagagggt
     6901 gaagtaattc aacaccctat gatcacaaaa tctatagaaa gagcacaacg caaggtagag
     6961 gaaaataatt ttggaatacg aaaacgtctt cttgagtacg atgacgtaat gaaccaacag
     7021 cgcgaggtga tataccgtcg ccgccgtaat gccctatttg gcgaacgcct cgaagtggat
     7081 atctcgaata tgatgtacga cctttgcgat caagtcattt ccgactatca ggatactggt
     7141 gatttcgagg agttcagact cgaagtttta agagatatgg ctatcaccgt gccatttgaa
     7201 aacaaggact ttcttcagga atccgttgat accttagccg aaaaactata taataaggtc
     7261 tatcagtcgt acaaactaaa gatgaaggcc attgctgaaa aggcagcgcc agtgatcaag
     7321 gatgtttttg aaaatcatac caacttcgaa aatattgcaa ttcctatcag tgatggtata
     7381 aagacaagaa acatcattgt taatttgaaa aaagcttatg aaaacggagc gcgtgaaatt
     7441 aatctggcca tcgagaagag tgttaccctc ggcatgattg acgaagcatg gaaagaacac
     7501 ctacgcgaaa tggacgatct aaaacaatcg gtacaggccg caacatacga acagaaagat
     7561 ccgctgttga tttataaatt cgaatcgttt cagcttttta agaggatggt acaaaggatt
     7621 aatcgtgaaa ttacctcttt ccttatcaag ggggatttac ctatccaaag ttcgggagat
     7681 gttagagagg ctcaattgcc aagaaggact gaccgccgta gaatgtcaga aagtagagat
     7741 gatctcctat cacaagctca tagcaacacg caacaacggc agaagccaga accagtaaag
     7801 gtagaaaaaa aggtggggcg caacgaaccg tgtccctgcg gaagtggcaa aaaatataag
     7861 cattgccacg gtagagctgg aggataagaa gctgcatcat aatgatttaa tactttgttt
     7921 ttaaaacttt ggctgaacaa aagtataaag ctgattaaag tggctgcagg cataaagcgt
     7981 tacgataagt tcactttatc ggcattcaaa atcgaacaga tttggaaaag cttaccctgt
     8041 aatatatttc gtaaactttt gtatgatagt tttatacttt gttatggtta aaaaatggat
     8101 atttgcagat catttttagg atggtttatt gattatcaaa acataaaatt aaaaacttca
     8161 atttataatt tactatgaac aatacattga gagagaagct aaccaaagta attcagcaac
     8221 acggcaatat ttttaacaac ataccgcgcc gtgagattat tcgtatggtt gttgaaaacc
     8281 gtgaagctat tattgccaaa tcgggagcgc ttgccacctg gaccaagaca gaatcaaatg
     8341 gtcgttcgcc aaaagatact gtttcggtta aacgtagttc gagtgagcat aatattgact
     8401 ggacttcgcc aaacaacatt ccgattgacg aagaaacttt cgatatgctt tttgatgatg
     8461 cgctgcagtt tatcagggat gcaaaacagg ttcaaattac cgatcgagtt attggcgccg
     8521 acagcaaata tgcactgccc gtaagagttg ttactccaaa tgctttacat gctcttttta
     8581 cggacaacat gtttcgccct ataccgaaag atatacatcg tagtaaattt tatcaaaatg
     8641 gatttgagtt aatagctgtt ccttatagaa aattgaatcc cgataaatat gaaggccgac
     8701 ttcgtaaatt gccaaatggt aaaacaacag acatggccgt tgcaatggat tttgacagga
     8761 ggcttggcgt tatcattggc tctgcttata tgggaagtgt taagaagatg ctttttacgg
     8821 tgatgaatta tctgcttcct ttagagggga ttttgccact tcattgttct gccaatgaag
     8881 gaccaaaagg tgattcagct ttactcttag gcctttcggg cacgggtaag actaccctgt
     8941 cggccgatcc gagtcgtgca ttgcttggcg atgatgagca tggctggagc gacgatggta
     9001 tcgctaactt cgagtttgga tgttatgcaa aaatgattga tatcaatcca aagaaagagc
     9061 ccgatattta tgatgcagta atgcatgttg atgactacct aaatcatggt tcgattgttg
     9121 aaaatgccat gatttatcct gatggcacat ttgattttta cgatgatcgc cttactccta
     9181 actcacgggc atccttcttg cttacttatc ttaaaaatat taaagagtcc tctacttcgg
     9241 gacatccaaa aacgattctg tttctaactg ctgatgccta cggtgtgtta cctcctgttt
     9301 ccagacttac cgaagacgag gcaatgcttt ggttcttaat gggctataca agtaaacttg
     9361 ctggtaccga aaccggtgtt actgagccac aagctacctt ttcgcgcttt ttcgggcagc
     9421 cattcatgcc ggcgaatcct gatgtttatg cagaaatgtt aggtaaaaag atgaaagaac
     9481 ataataccaa agtttatctg attaatacag gctggagtgg cggcgcttat ggaacaggtc
     9541 aaagaattga tctcacatta acccgaaaaa tggtaaatgc agctctttcg ggcgagttgg
     9601 aaaacgttaa ttataaacgc gataatctct tccatgtgga tgtgcctgaa aattgccccg
     9661 gagtgccatc agagatgctt tggccgaaaa atacatggaa agataaagag gcatacgata
     9721 aacaagctca gatattagca aaaaaattca gcgatgcgtt tgataaaaat tatgggcaaa
     9781 agaatataaa agagagcgtt ataaaagtat gcccgggtaa atagattaaa acactctttg
     9841 ttataattat gatccggctt ttgaaaaatt gccggatttt tttttaattt tgtcagaaaa
     9901 aaatatggga atcaatcaga atatcaaaaa gataaaaaat gaattacctg ccaatgttaa
     9961 gttaatcgcc gtttcaaaaa ctaagggaat agatgagatc atggaggctt ataatgccgg
    10021 acaacgacat tttggcgaaa ataaagtaca agagatgata gccaaacagc cacatctgcc
    10081 gtcagatatc cactggcatt ttataggcca tttacaaacc aataaagtga agttcatagc
    10141 cccctttgtg aatatgattg agtcagttga tcggcttaaa gttcttaagg agattaataa
    10201 gcatgctgca gctttgaata gagtaatacc gtgcttgttg caatttcata ttgccgaaga
    10261 agagtcgaaa tttgggttaa ccttatcaga atcttatgaa atacttgata acaattattt
    10321 tgacaaactt aaaaacgtaa gtatttgtgg cgtaatggga atggcaaccc ttactgatga
    10381 taaaacgcag ataagtaagg aatttaaaaa cctgaaaatc atatacgata ttctaaaaca
    10441 aaagtacttc caacatattg actcatttaa agaaatatca atgggtatgt caggtgatta
    10501 tctaattgca attgaacaag ggagtactat catccggttg ggtagcgtaa tttttggtca
    10561 acgagattag tgatttattc atccgtttga tttccgcact attaaataag atcattaaag
    10621 acctaattag attttcctcc ggtgcgaaac tgatcaacca atttttttta ttaacattgt
    10681 ttgttgaatc ctaaaaactt tagttctttg cgtagcttat tttaagactg aaatcttatt
    10741 ttaacagaca aaattgttat taaaaaatca ttattaagta atactaaaac ataggtacgt
    10801 gtgtttgaaa ttaaaaaaaa cagacctaat aaattctcat caagctggta agagtatgat
    10861 tatgattatg attgttcttc gtcttaatat ttttctgttt gatctaaaaa aatctaatgt
    10921 ttctgtttta aataacaact gactgttaaa tctcaaattt caataacttg aacaaaattt
    10981 tacctttaat attatggata tctacctgat tattattatt atacttattg cttttgcagt
    11041 tgccgacctt gctgtgggtg taactaatga tgccgttaac tttttaaatt ctgctgctgg
    11101 atccaaaact gccccaatgt gggtactatt aattgtggtg agctttggtg ttattttggg
    11161 agctaccttt tccagcggta tgatggagat tgctcaaaaa gggatatttc atccaggcca
    11221 atttacgttc tcagaggtga tgattatatt cttggcggtt ggtatggctg atataatttt
    11281 gcttgatctt tttaacactt ttggattacc tacatcaaca accgtatcca ttatttttga
    11341 attgttagga gcatccgttg caatatcgtt aataaaaatg aatatttcag gagaaagcat
    11401 tgtggaactt ggaaattata ttaattcacc taaagttctg gccattattt ccgccattct
    11461 tatttcggta ttccttggat ttattatagg cgctattata caatatgttg tcaggttaat
    11521 ctttaccttc aatttcgttc ccaaattaaa atattacggt ggtgtttttg ggggtctgtc
    11581 tattacagca attacctttt ttatgattct gaaaggggca aaaggagctt cttttttaac
    11641 tccggaaatg aatgaatggc tcgaaagtca tactattcac attcttttgt atggatttat
    11701 tttttggaca gtttttttgc aattactcta ttggattttt aaggtcccta ttacaaaaat
    11761 agttgtaata ataggcactt ttgcgcttgc ttttgccttt gcaggtaatg atttggtaaa
    11821 ctttatcggt gttccattgg cagggcttgc atcctacaaa agtttcttat caacaccggg
    11881 ggctactcct gatacttttt atatggattc gctttcacga gcggttacta ctgatacaat
    11941 ttatcttgtt attgcgggag taataatggc gctcactctt tggttctcaa aaaaggccag
    12001 aacggtaatt gctactgaag taaatttagc acggcaaggt tctggttatg agaggtttgg
    12061 tacttcagcc ttatcaaaat cagtagttag gggagtaata aaactcaata aatcattttc
    12121 agaaaaaata ccgaaaagaa tccaaaacag gattgataaa agatttgaaa ttccaaaaac
    12181 aatatatagc tctgtaaaaa caaaagattt accctcattt gatgaattaa gagcctctgt
    12241 aaatttgatg gtatcctctg tgttaatagc tttagggaca tccttaaaac ttcctttatc
    12301 cactacctac atcactttta tggtagccat gggaacctca ctctccgatc gagcctgggg
    12361 gcgcgacagc gctgtatatc gtgtgcaagg cgtattttca gtgattggag gatggtttct
    12421 tacagctgtt ctggcttttt ttatagcttt aatcattgct atagtgattt attttggcgg
    12481 gatcgtggct attggtgtaa tcgtaatatt agtgttattg tttgtctatc gtacttataa
    12541 gttgcacaga acaatggaaa aagaaagaac cgataatgct attgaacatg aaattgaaga
    12601 gataataagc gcatcggttg tttttgaaaa atgtatttca ttaacagtca atgctcttga
    12661 aaaagtacct gatttttata gacaactttt taaggggatt acagaagaag ataggaacag
    12721 cttgaaagag ttgaaaaagg agataaaaaa attcaataaa aaaactaaac gaataaaaga
    12781 taatgtaagt gaaacggtaa cccatctcca gattgatcag attgatacag gtcattatta
    12841 tgtacaaagt gttgattatc tgcgcgaaat agctcattgc ctcaactaca tggctaatcc
    12901 cgcatatgag cacattgata ataaccacac cggacttgat gagtttcaac agcaagaatt
    12961 tgaagcaatt tactcaggaa tatccgagtt gtataaggat attatagtta aaatacaaac
    13021 taaaaaatac agcgatctcg accaaattat tcttaaacag caggaaatat tgcaattgat
    13081 atccgaaagc cgtataaatc aggttaaacg attaaaagaa aaacaattac atacacgtgt
    13141 cagcttgctc tatttcgatt tgctggccga gacgaaaaac cttctcctct actctatcaa
    13201 tctcctgaaa tcgcaaagag atttcagaag tgagatattg aggagtcagc cgggttttta
    13261 tagtggctaa aaccagcgct gatattcaat aaatatctta ttgttgcagc ttcacagcgc
    13321 taagcatcct gtcatttccc gaaaggtctt ttttaattat tatatccttg aatccagagg
    13381 atgataacaa atcatacaac tcattgccaa agtgttgatt gatttcgaaa aaaatagttc
    13441 ctgatgattt caggtgatta atacaaaact gaagtgtttt acgatagtag atcaaagggt
    13501 cgtcatcatt gacaaaaagt gcgagcgatg gttcccaatt caatacgtta ggctgcattg
    13561 ccgctttttc cgaattagta acataaggcg gattactaat gattacatca tatacacacg
    13621 catttatcca tgaatcactt ttcaaaatat ctaaattaaa aaagtcaata ttcagtttca
    13681 ggtttttcgc gttttctccg gctaatatta gggcatcacg ggatacgtcg caagctgaga
    13741 tgtgagaagt aggaagcctt tttgccaaag caatagcgat acaaccacta cctgttccaa
    13801 tatcaaggat agagagattt tttttgttct gtaaactatc aactgcccac agaacaagct
    13861 cttcagtttc aggacgagga attagaacgt tttcattaac tttaagtgaa aatccgtcaa
    13921 accaagcttc accgaaaata tactgaatcg gacggtactt ttttaattct tttacatcaa
    13981 aatgaactat taacatctcc gattcgctca cccttaattc agggttcaat gcgagtgtcg
    14041 tcctgtttat cagtagtctg tgcgacaaaa ttaaatcgag aagcgccgtt gcctcatgaa
    14101 caggataata cgattgtagc tccttaatgt aggtttcacg tatttcttta actttgtttg
    14161 atcgaaaatc cacctgaaaa aataatatgg agaatatcta gacaaagtta gatgaaatta
    14221 ataaaactca gaaaattgga tcgttaagta ttatcacagt aacgagccgt taggcttctc
    14281 ccaagattgg aacgaaaacg atcgggattt gcttttaaaa taaatagttc cggtcaaaag
    14341 gactttgtat tgattaccct tttatttttc gtaaaaattt aaaaatcaat gtaaatcgtt
    14401 attcacaaag gcttctgttt ggattttatt ttacatttgt aaacaaattt tttacaaatc
    14461 atttttaaat gagaatcgga ttactaatca tattattttt cattccgcct ttattaatca
    14521 gtgcccagcc agaaaaatac acccgtattg agattccctg tagcttggaa aatattgaag
    14581 aggtttatcg tttagggata aatatagctt ttatagagcc tgacgattat ttgtggcttg
    14641 aagtttctga aggtgaattt gcccttctga agacaacagg aattgattat cgtattttga
    14701 tcgaagatat cagcgaattt tatcgtagtc gtaacagtaa ta
//
LOCUS       DAOOAI010000054        14557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_5348_length_14557_cov_4.840781, whole genome shotgun sequence.
ACCESSION   DAOOAI010000054 DAOOAI010000000
VERSION     DAOOAI010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14557
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5348_length_14557_cov_4.840781"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(154..1149)
                     /locus_tag="PK345_05285"
     CDS             complement(154..1149)
                     /locus_tag="PK345_05285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005676213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPX34463.1"
                     /translation="MNNLLTISGSPHIHTTDSVRKIMYSVIFAMIPAMLVSVYFFGLD
                     AVRIIGISVVACVAVEYLIQKYLLKGDVTINDGSAVITGVLLAFNVPANLPTWIIIIG
                     AIFAIAIAKMAFGGLGKNPFNPALVGRVFLLISFPAQMTTWPIPKPLFAKSVTDALTG
                     ATPLGLLKEGHLANKTIDQIMSDPAMPDYIQQLVGFQTGSLGEMSAIALLIGAAYMFY
                     KKVITWHIPVSYLLTALLFAAIFWLIDPTYYADPLFHLITGGMILGVFYMATDMVTSP
                     MSAKGQIIFGIGCGLLTMIIRFWGSYPEGVSFAILIMNAFTPLINKSFKPKRFGV"
     gene            complement(1153..2487)
                     /gene="rsxC"
                     /locus_tag="PK345_05290"
     CDS             complement(1153..2487)
                     /gene="rsxC"
                     /locus_tag="PK345_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006745403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HPX34464.1"
                     /translation="MLKTFKLGGVHPPENKISAGAAIQHLPLPDKLFVPLSQHLGTPA
                     KAIVNRGDDVKTGQLIAKSVGFISANIHSPATGKVAKIDQIIDQTGYKRTAVFIDTQE
                     DVWEEGIDTSPDIERTIKLSKEEIIQRINDMGVVGMGGATFPTHIKMMIPEGKQIQYL
                     IINGVECEPYLTSDHILMMEKGEEILIGTSILMKALGVDKSMVGIEANKPDAIAHLTK
                     LTEKFQGITVHALKVKYPQGGEKQLIKALTGREVPSGKLPLETGCVVDNVGTAYAVYE
                     AVQKNRPLIQRVVTVTGNAARKHANFLVRVGTPIEYLIEHAGGMPADLGKLISGGPMM
                     GKALVSPEAPITKGSSGLLLMPETEAKRAPIKNCIRCARCISACPMGLEPFLLAQLVP
                     HDKWDDAEANRIMDCIECGSCHYICPSGRPLLDFIRLGKDKVGKIIRTRVKQ"
     gene            complement(2526..3413)
                     /locus_tag="PK345_05295"
     CDS             complement(2526..3413)
                     /locus_tag="PK345_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008022714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit B"
                     /protein_id="HPX34465.1"
                     /translation="MDNIILNAVIALSGIGVTAAVVLYFVAQKFKVIEDPRIDVVEAI
                     LPGANCGGCGFAGCRAFAEAIVAAGNLDNLNCPVGGNTAMAEIAKVLGLEAEEKEPLI
                     AVLRCNGSLTNAKPKVKYDGPLTCAFAHNLYSGESGCPFGCLGLGDCVDSCKFDALYM
                     DKMTGLPVVIQDNCVACNACVKACPRGIFELRPKGKKDRRIYVGCINEEKGVPAKKNC
                     EVACIGCGKCVKECQFDAITLINNLAYIDPYKCKLCRKCALVCPTNSIHELNFPPRKE
                     KLVEKVDAPTEPAPVATEG"
     gene            3869..4057
                     /locus_tag="PK345_05300"
     CDS             3869..4057
                     /locus_tag="PK345_05300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34466.1"
                     /translation="MKLYETLEQQLKKEPNFVTDNGELKKYNQTFIEQIEALKPDGTN
                     EEKRFVELFSRRFEGNQD"
     gene            complement(4282..5514)
                     /locus_tag="PK345_05305"
     CDS             complement(4282..5514)
                     /locus_tag="PK345_05305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside recognition domain-containing
                     protein"
                     /protein_id="HPX34467.1"
                     /translation="MVLNYIWIAFFIVAFVIGLIRLLFFGDVDIFTEMMGSTFEMAKT
                     GFEISLGLTGVMTLWLGLMKIGENGGVVKIMAKLVGPLFHKLIPEIPKNHPVTGSIMM
                     NLAANMLGLDNAATPLGLKAMTQLQELNHQKDTASNAMIMFLVLNTSGLTIIPISIMV
                     FRAQLGAADPTDVFIPILLATFFSTLAGIISVAVIQKINLFNRIILAYLGSFTLIIAL
                     IIWYFTTLTQEKIGLLSSLAGNFILFSVIISFIVLAVRKKVNVYESFIEGAKEGFTIT
                     IKIIPYLVAMLVAIGVFRTSGAMDFVIKAIGHGFAKAGINTDFVAALPTAFMKPLSGA
                     GARGMMVDAMNTFGADSFVGRLASVFQGATDTTFYIIAVYFGSVAIKKTRYAITCGLI
                     ADLAGIVAAIIIAYIFFH"
     gene            complement(5533..7182)
                     /locus_tag="PK345_05310"
     CDS             complement(5533..7182)
                     /locus_tag="PK345_05310"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HPX34468.1"
                     /translation="MNKLTKLIFVMSALSLFSCSPKEKADLVILNATAYTVDEDFSIV
                     EAVAVKDGKIVALGSSSEISDRYSSSCIIDLDKKALYPGFIDAHCHFYSYGLGLLQRA
                     DLTGTESFEKVLEIMENHRHKFPSIQWLQGRGWDQNLWIDKTFPDNDELNKLFPENPV
                     ILTRIDGHAALVNQKALELAGIDAATKISGGEVALKNGQPTGMLIDNAIGLVTRVIPK
                     PDATMISEALLTAQHNCFAAGLTGVHEAGLTKEIIKIIDSLQKAGLLKMRVYAMLSPT
                     QETLDAYFKNGITKNENLNIGSIKLYADGALGSRGAKLIDDYSDNSGNSGLLVENPET
                     LKKYCQMAYDNGFQVCTHAIGDSANRLMLTIYGDILKNKNDLRWRIEHAQVVAPSDFD
                     LFGKYSVIPSVQPTHATSDMFWAGDRLGAERIKGAYAYNDLLRQNGWMPLGTDFPIEN
                     INPIHTFYASVVRKNLDGKPETGFQMENAISREEALRGMTLWAAKAAFEENEKGSISV
                     GKLADFVILDKDIMTIAENEILNVKVLMTIVSGETVFLSEQ"
     gene            complement(7179..8177)
                     /locus_tag="PK345_05315"
     CDS             complement(7179..8177)
                     /locus_tag="PK345_05315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX34469.1"
                     /translation="MIELNGVDIGYLTGHDKSGVLHQDINLELNLGEFVCMIGPNGAG
                     KSTLIRTISGLQKPLKGNILLDNRNIYQTPPAFLARLISVALTAMVEISNASVYDMVA
                     YGRSPYTNFFGKLSKHDHVIIEKSLESAGIAHLASRKFDTLSDGERQKVLIAKSLAQE
                     TPFIFLDEPTAFLDFPGKVEIMQLLREAAWIHGKSILLITHDINLAIQFADRLWLMAR
                     HKPIITGIPEDLVLTKKIGLFFDKEKIKFEVEHGNFIFETNLKGKIILSGQGITHNWT
                     AKALRRKGFEVIAGSEVKSGVPFIHISDGVIKLIFKEKTFCFNTIEELIKLLDQLQ"
     gene            complement(8186..9169)
                     /locus_tag="PK345_05320"
     CDS             complement(8186..9169)
                     /locus_tag="PK345_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008152656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HPX34470.1"
                     /translation="MFVLNLLLGSVSIPVLQVVRTLLGMEVKDLSFVTIILQSRLPMA
                     ITAMLAGAALGVAGLMMQTLFRNPLAGPSVLGVSSGASLGVALVMLFIAIPGGSFVAV
                     SQISGKYSVIIAAFVGSSIVLFIISLLASRFNSNTIILIIGIMIAYATSSLVGILQFY
                     SLKEDLQAFVIWGLGSFGNVPLSLITTFALLTAIGLTLSVLLIKPLNLFLLGEYYAIN
                     LGLNTKKSRYWILITTGLLTATVTAFCGPIAFLGLAVPHIARIIFQTSDHRIILPGSL
                     LCGALLALVCNIIARLPGIDSALPINAITSLIGAPIVIWLLVKKNTMKIVD"
     gene            complement(9250..10422)
                     /locus_tag="PK345_05325"
     CDS             complement(9250..10422)
                     /locus_tag="PK345_05325"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HPX34471.1"
                     /translation="MKMHNQREAYLFFKREIIKIPIYFFFLIIFASCHSPSAPIDNEE
                     ATPDELNPTFAKGFQILQGKGFKILNLYSSDGTTLIDHFILADRFDSQLVKGLVGKKI
                     IQTPVESVVCLSATHIAFADALGVIDRVTGVSSAHYVVSDRFAELVNDGKIKEIGIGD
                     HFKLEELISLNPNLILVSMQYGQNFETLRNAGLMILPIAEHLENDPLGRSEWIKLFGV
                     LTGKSKMAMDMFDSIVHQYNELKELASDVNFRPTVLSGKQYGGFWNLPGGKSYLSQMI
                     SDAGATYMFNDNDESGSFVLDFETVYHIGVNADYWRILIFARDTYTFQDLLDEDMRYA
                     GLAAFKNKKIFYCNTFLKPYFQKSPLEPHLLLADYISIFHPELLPDYTRSYYSILK"
     gene            complement(10434..10811)
                     /locus_tag="PK345_05330"
     CDS             complement(10434..10811)
                     /locus_tag="PK345_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005859009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES family protein"
                     /protein_id="HPX34472.1"
                     /translation="MTDAQGLSKLIVVGDRVLIKPKAYSQKTSTGLYLPPGFKEKEEV
                     QTGFVMKVGPGYPIPSANDEIDEPWKKNEEKIRYIPLQAKEGDLAIFMQKGAVEVMFH
                     GEKYFIVPHHSILLLERDEELFS"
     gene            10883..11143
                     /locus_tag="PK345_05335"
     CDS             10883..11143
                     /locus_tag="PK345_05335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34473.1"
                     /translation="MRIVFFKRPKPKRFEYKPRYWDEEKERLEERKKHLGEESSERNT
                     LIRDINLHWRKIDRRNRNKAKSINLVVYLFIILLLLYFIFKA"
     gene            11161..12999
                     /gene="mutL"
                     /locus_tag="PK345_05340"
     CDS             11161..12999
                     /gene="mutL"
                     /locus_tag="PK345_05340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HPX34474.1"
                     /translation="MPDIIHLLPDSVANQIAAGEVIQRPASAVKEMLENAIDSGADKI
                     ELVIKDAGKTLIQITDNGCGMSETDARLSFERHATSKISSVQDLSSIRTMGFRGEALA
                     SITAISHVEMKTKRHGAELGTHIIIEASEVKSQEFCQCPPGTTIMVKNIFFNIPARRK
                     FLKSDNVELRNIIEEFQRIALANPEISFSLYNDRKIIFQLSPSNLKQRIVNIYNTTYN
                     RRLIPVEESLNIVKVNGFIGKPEFARKQRGEQYFFVNKRFIRHPYLNHAVENAYDELL
                     PEKSFPSYFLFLEIDPAIIDVNIHPTKTEIKFEDEKIIYSVLKSAVKAALGKFSITPA
                     IDFDVEPLFDFPTRTKDASTKQPTVGFNPDYNPFEKRQAPLTPRQISNRDNWDKMIPP
                     KDFSFGKETDSNTDSVPKLIEDDNSDMAIQLPESNFLQLHRKYIITQVKSGLMIIDQQ
                     RAHERIMYERYLELLSKRVTISQQVLFGVKISLSAGDSELLNEIMEDLNLLGFNIKAD
                     KNKPFQYIAEGIPAGTEIENVPDTIDRILHNYKQGLIEFAQSKRVNLARSMAKNLSVK
                     TGKVLQQAEMKTITEELFSCQAPEVSLDGEPTVKVININKYFNHKE"
     gene            13056..13907
                     /locus_tag="PK345_05345"
     CDS             13056..13907
                     /locus_tag="PK345_05345"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPX34475.1"
                     /translation="MNYQQYSPQRFNVLPPVVKNLLIINGLFFLATIALHKSFGIDLA
                     SYLGLRFPGSQAFNPYQFVTYMFMHGSLSHIFFNMFALWMFGYTLENIWGSKRFLIYY
                     LVTGIGAGLVHYGTLYLEYRPLIITLNGIIDDPSVENLVNFRASIQGTISPYAGEIFL
                     NYNNFIKALNQLIQKPESQALIESARGFVIDYKQYMLSRPNVIGASGAVFGILLAFGM
                     LFPNVRLYLYFFFPIKAKWFVILYGLLELYLGFTQSHSNIAHFAHLGGMVFGFILIKY
                     WKHKRIY"
     gene            13910..>14557
                     /locus_tag="PK345_05350"
     CDS             13910..>14557
                     /locus_tag="PK345_05350"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HPX34476.1"
                     /translation="MFAYQNPLDNIKKFFKSKSPLVYLIYINLGVLILVHLTNVVCWL
                     FEIKNHAGTNANVSLLTYYLSLPADLGQISLRPWTLLTYMFLHENILHFLLNMIVLYF
                     GGRIFLEYLTERKLLSVYILGGIAGALFYIGAFNFFPVFKGSISDSIALGASASVLAI
                     LFAIATYVPNYIIVLFIFGRVKLKYLALIIVLVDILSIPLGNAGGHIAHLGGAFWG"
BASE COUNT         4497 a         3037 c         2681 g         4342 t
ORIGIN      
        1 tgggtctttg gtaagattgt acacataact tgccgcaagg gaggataccg ctgttgaaat
       61 taaaagcgtt aaaaacatat ttaaaaatgt agattccagt tttgccatat caaaatattt
      121 tttttgatgt cgttattatt tattattacc gagttataca ccaaaacgtt ttggtttgaa
      181 acttttatta attagtggcg taaaggcatt cataataaga atggcaaaag aaacgccttc
      241 gggatagcta ccccaaaatc taatgatcat tgtaagtaat ccgcagccta ttccaaaaat
      301 tatctgtcct tttgccgaca tgggcgaggt taccatatcg gtagccatat aaaagacccc
      361 gagtatcata cctccggtaa tcaagtgaaa tagaggatca gcataatagg tagggtcaat
      421 aagccaaaaa atggcagcga atagcagcgc tgtcaataag taggacacag ggatatgcca
      481 tgtgattacc tttttataaa acatgtacgc tgcgccaatc aacaaagcaa tagctgacat
      541 ttcgccaagt gagccggttt gaaatccaac caactgttga atataatcgg gcatagctgg
      601 atcgctcata atctgatcaa tggttttatt ggccaaatgc ccctctttaa gtaatcctaa
      661 tggagtagcg ccagttaacg catcggttac tgattttgca aataatggct ttggtattgg
      721 ccatgtcgtc atttgtgcag gaaacgaaat caataaaaaa acacgaccaa cgagcgccgg
      781 gttaaaagga tttttgccca atccgccgaa agccatttta gctattgcta ttgcaaagat
      841 agcgcctatt ataattatcc aagttggcaa gttggcgggc acattgaagg caagaagaac
      901 tcccgttatt acagcagagc catcgttgat tgttacatct cctttaagaa gatatttctg
      961 gataaggtat tccacagcaa cacaggcaac aactgaaatg ccaataattc gtactgcatc
     1021 gagcccaaaa aagtaaaccg agaccagcat tgccggaatc atggcaaata tgacgctata
     1081 catgattttg cgaacggagt cggtggtgtg aatatgtggc gaccccgaaa tggtgagtag
     1141 attattcatg atttactgtt ttaccctggt tctaataatt tttcccactt tgtccttacc
     1201 aagccgtata aaatcgagca atgggcgccc tgatgggcat atataatgac atgatccaca
     1261 ctcaatacag tccattattc tgttagcttc ggcatcatcc catttatcgt gtggtacgag
     1321 ctgtgctaag aggaatggct caagccccat cggacaggcc gaaatacatc ttgcacatct
     1381 gatacaattc tttataggcg cacgcttagc ttctgtttcc ggcattagca gcaaccccga
     1441 cgagccttta gtaataggag cttctggtga aactaatgct ttccccatca ttggccctcc
     1501 gcttatcaat tttcccaaat ccgcaggcat accacccgca tgttctatca aatattcaat
     1561 aggcgttcct actcttacca aaaaattagc atgtttacga gctgcatttc ctgttacggt
     1621 aactacacgt tggatcagtg gtctattttt ttgaacagcc tcatatacag catatgcagt
     1681 tcctacatta tcaaccacac agcccgtttc caaaggtaat tttcctgagg gcacttcccg
     1741 accggtaaga gccttgataa gctgcttttc accaccctgc ggatatttta ctttcaatgc
     1801 atgcactgta ataccttgaa acttctcggt taatttagtc agatgcgcaa tagcatcggg
     1861 cttgttagct tcaataccta ccatcgactt atccacgcca agagctttca tcaatattga
     1921 agttccgata agtatctcct cacctttctc catcatcaga atatggtcgg aagttagata
     1981 tggctcacat tctacgccat tgataatcag atattggatc tgtttgcctt ccggtatcat
     2041 cattttaata tgagttggga aggtagcccc acccatacca acaacaccca tatcgttgat
     2101 acgttgtatg atctcctctt tgctaagctt aatggttctt tcgatgtcag gtgaagtatc
     2161 aattccctct tcccaaacat cttcctgagt atcaataaaa actgctgttc gcttatagcc
     2221 ggtttgatca atgatctgat ctattttggc cacttttcct gtggcaggag agtgaatatt
     2281 tgcagagata aaaccgacgc ttttagcaat cagttgtccg gttttgacat catctccacg
     2341 attgacgatt gcctttgcag gcgttccaag atgttgggaa agcggaacaa ataatttatc
     2401 cggcaaaggc aaatgctgaa tggcagcgcc tgctgaaatt ttgttttccg gcgggtgcac
     2461 acctccaagt ttaaaagttt ttaatatcat ttttcgtcta tattaggata acgaaatttt
     2521 tgcgattaac cttcagtggc aactggagca ggctcagtag gggcatctac tttttctaca
     2581 agcttttcct ttcgtggcgg aaaatttaac tcatggattg aattggttgg gcatactaat
     2641 gcacatttgc gacacagctt gcatttatat gggtcaatgt atgcaagatt attaatcagt
     2701 gtgatagcat caaactgaca ctccttcaca catttaccac agccgataca tgctacttca
     2761 caatttttct ttgccggaac gcctttctct tcatttatac aaccaacata aatccggcga
     2821 tcttttttcc ctttaggacg aagttcaaat atgcctcttg gacacgcttt tacacaggca
     2881 ttacaagcaa cgcaattatc ttgtatcact accggcaaac cagtcatttt atccatgtaa
     2941 agagcatcga atttgcagga atccacgcaa tcgcctaatc ccaagcagcc gaatgggcat
     3001 ccgctctctc cagaatatag gttgtgtgca aaagcacatg tgagcgggcc gtcatatttt
     3061 acttttggtt ttgcgttggt cagtgagcca ttgcagcgta atacagcaat taaaggctcc
     3121 ttttcctctg cttccaaccc taataccttt gctatctctg ccattgctgt attgccgcca
     3181 accgggcagt ttagattatc caaattacct gcggctacta ttgcttcagc aaaagcacga
     3241 cagccggcaa aaccacagcc gccacagtta gcgccgggta aaatagcctc aacgacatca
     3301 atcctcggat cttcgataac cttgaatttt tgagctacaa aatagagcac tacagcagcc
     3361 gtaaccccaa ttccactcag ggcaattacc gcattaagaa ttatgttatc catgagagcg
     3421 ctgttttgtc atgtaattaa ttcaatctca cttagttatt tggataataa aaataatctg
     3481 aacaaatatt tgctcacttt tgtccaatta actttgacgc aaaattatat catacaccaa
     3541 tcgtttatac actatttctc aatttaaaat gattataaac ttacaactat tcaaacataa
     3601 cttttaagct tcgaaatgaa tagttaggcc aatatcacat taagggaaag ctgaaaaaaa
     3661 aagagaacga caatggtaaa tttgctgcga aaaggctgaa aagaaaaccc aacgtccatt
     3721 caaccaaaag agtcgtcatc ttgccatcgc actcaagccc gtcccccacc cttcaaaaat
     3781 tagttgacaa gaattatgga aaacataaag attaacaata caaaaaaaaa agtatttttg
     3841 aaatctaaga tttaaggatt tgaaataaat gaaactatac gaaacattag agcaacaact
     3901 aaaaaaagaa cccaacttcg taacagacaa cggagaactg aaaaaataca accaaacttt
     3961 cattgaacag atagaagcat taaagccaga cggcacaaat gaagaaaaga gatttgttga
     4021 actgttttcc agacgttttg aaggaaatca ggactgaaca aaagacaatc aaaatccaca
     4081 cggacaccta tttgataaaa gccgttccgt tttacaatta cgttaacgaa aacgaattca
     4141 agacaatatt agaaaacaca ttaaaagttt agaattgtca actttcgtgt aggagggtgg
     4201 aaactgctac acaatcactt acggtacata ctctcaatcg ttgctctaaa tattgatcgg
     4261 gtaattgagg cagatgtcca attaatggaa gaaaatataa gcaataataa tagctgcaac
     4321 aattccggcg aggtcagcta tcaatccgca ggtaattgca tagcgtgttt ttttaatagc
     4381 taccgaacca aaatagactg ctatgatata aaaagtggtg tcggttgctc cttggaacac
     4441 cgaagctaac ctcccgacaa aagaatcagc gccaaaagta ttcatagcgt ccaccatcat
     4501 tcctcgagct cccgcaccgc taagtggttt cataaatgct gttggcaagg ctgcaacaaa
     4561 atctgtatta atccctgctt tagcaaaacc atgacctatc gctttaataa caaaatccat
     4621 tgcgcccgat gtcctgaaaa cgccgatagc tacaagcata gcaactaaat agggtataat
     4681 ctttatagta atagtaaaac cctcttttgc tccttcgata aaggattcgt acacgttcac
     4741 tttttttcgt actgccaaaa caatgaaaga gatgataacg gaaaaaagaa taaagttacc
     4801 ggctaatgag gataaaagac cgattttctc ttgagtaagg gttgtaaaat accagattat
     4861 taatgctata attagagtaa aactaccaag ataggccaga attatcctgt tgaagagatt
     4921 aatcttttga ataactgcaa ctgaaataat ccctgccaag gtggagaaga aagttgctaa
     4981 tagaatagga ataaaaacat cggtgggatc agcagcgcct aactgagctc taaaaaccat
     5041 aatgctgatc gggataatgg tcagaccgga agtattaagg acaaggaaca ttatcattgc
     5101 attactagcg gtatctttct gatggtttaa ctcttgcagt tgagtcattg ctttgagtcc
     5161 gagcggtgtg gcggcattat caagtccaag catgttggca gccagattca tcattatgga
     5221 acctgttacc ggatggtttt ttggaatttc aggaataagc ttatggaata atggcccgac
     5281 aagttttgcc attattttta ctacgccacc attttcacct attttcatca atccgagcca
     5341 aagggtcatc actccggtaa ggcccaagga gatttcaaag cctgttttgg ccatttcaaa
     5401 agtacttccc atcatttcgg tgaagatatc cacatcacca aaaaaaagaa gccttatcaa
     5461 gcctataaca aaggcaacaa taaaaaaagc gatccagata taattgagaa ccatgctttt
     5521 atatttttct agttactgct ccgaaagaaa aactgtttca cccgacacaa tcgtcatcaa
     5581 aacttttaca tttagtattt cattctccgc tatagtcata atatctttat caaggataac
     5641 aaaatcagca agtttgccaa cacttatact ccctttttca ttttcttcaa aagctgcttt
     5701 tgcagcccat aatgtcattc ctcgcaaagc ctcttctctt gatattgcat tttccatctg
     5761 aaatcctgtt tcaggtttac catcaagatt tttgcgaaca acagaggcgt aaaaggtatg
     5821 tatcggattt atattttcta taggaaaatc tgttcctaat ggcatccatc cattttgccg
     5881 taacagatca ttataggcat aggctccttt tattcgttcc gcccctaacc tatcgccagc
     5941 ccaaaacata tcagaagttg catgtgtagg ctgaaccgat ggtatcacag aatatttgcc
     6001 aaagaggtca aaatcggagg gagcaacgac ctgagcatgt tcgatgcgcc agcgcaaatc
     6061 atttttgttt ttcaaaatgt ctccataaat cgttaacata agcctgttag ccgaatctcc
     6121 aatagcatgt gtacatacct gaaaaccatt gtcatatgcc atttggcaat atttttttaa
     6181 tgtctccgga ttttccacca gcagccctga gttaccagaa ttgtcgctat aatcatctat
     6241 tagtttggct ccacgcgaac cgagggctcc atcagcatac aacttaatag agcctatgtt
     6301 aagattctca ttttttgtta tcccgttttt aaaataagca tccaaagtct cttgtgtagg
     6361 tgaaagcatg gcgtaaactc tcattttcag aagaccggct ttttgtaacg aatcaataat
     6421 tttgattatc tcttttgtaa gtcctgcctc atggacaccg gtaagccctg cagcaaaaca
     6481 gttatgctgc gccgttagca gcgcttcaga tatcattgta gcatcgggtt ttggaataac
     6541 ccgagttacc agaccaatag cattatcaat caacattcct gttggctgac cattttttag
     6601 cgccacttcc ccaccgctga tttttgtcgc ggcatctata ccggctaact ccaaagcctt
     6661 ttgatttacc aaagcggcat gtccgtcaat tctcgttagg attacaggat tctcaggaaa
     6721 aagtttattc agctcatcat tatcggggaa ggttttatca atccagaggt tttgatccca
     6781 accgcgtcct tgaagccact gtatggaagg aaatttatgc ctatgatttt ccattatctc
     6841 caataccttt tcaaaagatt cagttcctgt aaggtcagcg cgctgaagca aaccaagacc
     6901 ataactataa aaatgacaat gtgcatcaat gaaacccggg tacagcgctt ttttatctaa
     6961 gtcaataatg catgaggagc tatatctatc ggaaatctca gacgagctac ccaatgcgac
     7021 aatcttgcca tccttaacag caacagcttc gacaatagaa aaatcctcat ctacggtata
     7081 agcagtcgca tttaaaataa ccagatcagc tttttctttt gggctacaag aaaatagaga
     7141 gagagcgctc atcacgaata tcagttttgt aagtttattc attgtaattg atcaagtagt
     7201 tttattaatt cttcaattgt attgaaacaa aaagtttttt ctttaaaaat cagttttatc
     7261 acaccgtcag atatatgaat aaacggcacg ccggatttta cttcagagcc tgcaattacc
     7321 tcaaacccct tccgcctcaa tgcttttgct gtccaattgt gtgttattcc ttgtcctgag
     7381 agaatgattt ttccttttaa attggtttca aagataaagt ttccatgttc aacttcgaat
     7441 tttatttttt ctttatcgaa aaacaaacct atcttttttg ttaaaacaag gtcttccggt
     7501 attcccgtaa ttattggttt atgtcttgcc ataagccaca gcctatcggc aaattgtata
     7561 gctaagttaa tatcatgggt aattaacaag attgattttc cgtgtatcca tgctgcttca
     7621 cgcaacagtt gcattatttc tacttttccg gggaagtcga ggaaggctgt aggttcatca
     7681 agaaagataa atggcgtttc ctgtgctaac gatttggcga tcagcacttt ttggcgctct
     7741 ccatcgctta gtgtatcaaa ttttcgtgag gccaaatgag ctataccggc gctttcgagg
     7801 gatttttcaa taataacatg atcatgcttt gacaatttcc cgaaaaagtt agtatagggc
     7861 gatcggccat acgccaccat atcataaaca cttgcattac taatctcaac catcgcagta
     7921 agcgcaacac ttatcagcct tgccaaaaaa gccggaggag tttggtaaat gttccgatta
     7981 tccagaagta tgttaccttt taaaggtttt tgcagcccgc tgatggttct tattaatgtt
     8041 gatttccctg ctccatttgg ccctatcata cacacaaatt caccgaggtt taactctaaa
     8101 ttaatatcct gatgcagaac acctgatttg tcgtgtccgg tcaaatatcc aatatctact
     8161 ccgttaagtt caatcataat ttcgattaat caacgatttt cattgtgttt tttttcacta
     8221 aaagccaaat cactattgga gcgcctatga gtgaagtaat ggcatttata ggcagtgcgc
     8281 tgtcaattcc gggtaatctt gcaataatat tacatacaag cgccagcaaa gcgccgcaaa
     8341 gtaacgaccc tggtaaaata atcctatgat cggaagtttg aaaaataatt cgtgcgatat
     8401 gtggaactgc aagtccaaga aaggctatcg ggccgcaaaa agctgtaaca gtggcggtaa
     8461 ggagtcctgt tgtaattagt atccagtatc ttgatttctt tgtgttcaac ccaagattaa
     8521 ttgcataata ttctcctaac agaaagaggt ttaagggttt gatcagtaaa acagagaggg
     8581 taagtccaat agctgtcagt aatgcaaaag tggtgataag ggaaagcggt acgtttccaa
     8641 agctgccgag gccccatatt acaaaagcct gtaaatcttc tttcaagcta tagaattgca
     8701 aaatacctac taatgagcta gttgcataag cgatcatgat tccgataatc aaaataatgg
     8761 tattgctatt gaatctggaa gcaagaaggc ttatgataaa caatacgata gatgaaccta
     8821 cgaaagcagc tattattacg gaatatttcc cactgatctg gcttacggca acaaatgacc
     8881 ctcctggtat ggcaataaat aacataacga gtgcaactcc taaacttgca cccgagctta
     8941 cgccaagcac tgaaggaccc gcaagcggat tgcggaaaag tgtctgcatc atcaggccag
     9001 ctacaccaag cgccgcaccg gcaagcatag ctgtaatagc cattggcaac ctcgactgaa
     9061 gaattattgt aacgaaagat agatctttaa cttccatacc caaaagagtc ctgaccactt
     9121 gtaaaacagg aatggaaacc gaacccaaca gtaaattaag cacaaacatc gctataatca
     9181 ttaatgctaa caaaatgaat atcaaactgt taacacgtga attaaggtga tatttatatt
     9241 ttgaccggtt cactttaata tcgagtaata gcttcttgta taatcaggta aaagttcagg
     9301 atgaaaaatg gagatatagt cggcaagcag cagatgaggt tcaagggggc ttttttgaaa
     9361 atagggtttt aaaaatgtat tgcaataaaa gattttctta tttttaaaag ctgctaatcc
     9421 tgcatatctc atatcttcat caagcaaatc ctgaaaagta tatgtatctc tcgcaaagat
     9481 aagtatgcgc cagtaatcgg cattgacccc aatgtgataa actgtttcga aatctaatac
     9541 aaagctgcct gattcatcat tatcgttaaa catataggtt gcgccggcat ccgaaatcat
     9601 ctgtgacaaa taacttttcc caccgggtaa attccagaac ccgccatatt gtttacctga
     9661 taataccgta ggacgaaagt tgacatcact tgccaactcc ttaagttcat tgtattgatg
     9721 aacaatagag tcaaacatat ccattgccat tttacttttt cctgtcaaaa ctccaaaaag
     9781 ctttatccat tccgatcgtc caagaggatc attttcaaga tgttctgcaa ttggcaatat
     9841 cataagaccg gcattgcgca aagtctcaaa attttgcccg tactgcatac ttacaagtat
     9901 cagatttgga ttaaggctaa tcaactcttc cagtttaaag tgatcgccaa taccgatctc
     9961 ttttatcttt ccatcattta ccaattcagc aaaacgatct gatacgacat aatgggcgct
    10021 tgatacacct gttacccgat caatcacacc gagagcatcg gcaaaagcta tatgagttgc
    10081 cgacagacaa acgacacttt ccacaggtgt ttgtataatt tttttaccga caaggccttt
    10141 taccaattgt gaatcgaacc tatccgctaa aataaaatga tcaattagag tggttccatc
    10201 gctggaatat aaattgagaa ttttaaatcc tttgccttgc aaaatttgaa aaccttttgc
    10261 aaacgttgga tttaactcat cgggagtagc ctcttcgtta tcaattgggg ctgaaggaga
    10321 atggcatgaa gcaaaaatta ttaaaaagaa aaaataaatg gggattttta taatctccct
    10381 cttaaaaaaa aggtatgctt ctcgttgatt atgcattttc ataaaaagca tgcttaacta
    10441 aaaagctcct catcacgttc aagtaacaaa atggaatgat gtggtactat aaaatatttc
    10501 tctccatgaa acataacttc cacagctcct ttttgcataa agattgctaa atcaccctcc
    10561 tttgcctgta gggggatata ccttattttt tcttcatttt tcttccatgg ctcatcaatt
    10621 tcatcgttag cggaaggtat tgggtaaccg ggccctactt tcataacaaa tcctgtttgc
    10681 acctcttcct tttccttaaa accaggtgga agataaagcc ctgtgctggt cttttgcgaa
    10741 tatgctttcg gcttgatcaa cacacgatcg ccgactacaa tcaatttact taacccttga
    10801 gcatctgtca taagaattgc atttcgtttt acggacaaag atacaaatat agtatttttg
    10861 tgagctaaaa tcactaatca ccatgcgtat tgtatttttc aaaaggccta agcccaaacg
    10921 ttttgaatat aaacctcgat attgggacga agaaaaagag cgtcttgagg agcgtaaaaa
    10981 acatctggga gaagaaagtt cggaaagaaa tactctcatc cgtgatatca accttcactg
    11041 gcgtaagatt gaccgaagga acaggaacaa ggcaaaaagt atcaatcttg tggtttacct
    11101 ttttatcatt ttgcttttgc tctatttcat ttttaaagcc tgaataataa gtattttatc
    11161 atgcctgata ttattcatct tttacccgac tctgttgcta accagatagc tgccggcgag
    11221 gtcattcagc gtcctgcgtc agcagtaaag gagatgctcg agaatgccat tgacagtgga
    11281 gctgataaaa ttgaattagt aatcaaggat gccggaaaaa cgctcattca aattaccgac
    11341 aacggttgcg gcatgtcaga aactgatgcg cgattatcat tcgagagaca tgctacctca
    11401 aagattagtt ctgttcaaga tctatcttca attcgcacga tgggctttcg cggagaggcg
    11461 ttagcctcta tcaccgccat ttcgcatgta gagatgaaga ctaaaaggca tggggcagaa
    11521 ttgggcacac atattataat tgaagcttcc gaggttaaaa gtcaggagtt ttgccaatgc
    11581 cctcccggaa cgacaattat ggtaaagaat atctttttca atattcctgc caggcgtaaa
    11641 ttccttaaat cggacaacgt tgagctgcgt aatatcattg aggaattcca acgtattgct
    11701 ttggcgaatc ccgaaatttc gttcagcctg tacaatgacc gtaagataat ttttcagctt
    11761 tcaccttcca atcttaagca gcgcattgta aacatatata atacaacata caaccgacgc
    11821 ttaatccctg ttgaagaatc tcttaatatt gtaaaagtca atgggtttat tggaaaaccc
    11881 gaatttgcac gtaaacaacg cggtgagcag tatttttttg ttaacaagcg ttttatcaga
    11941 catccttatc taaatcatgc agtagagaat gcctacgacg agctgctacc tgaaaaatca
    12001 tttcccagct atttcctttt tctcgaaatt gatccggcta tcattgacgt caatattcat
    12061 cctacaaaga cagaaataaa atttgaagat gaaaagatca tttattccgt attaaaatca
    12121 gccgtaaaag ctgctcttgg aaaatttagc attactccgg ctattgactt cgatgttgag
    12181 cctttatttg atttccctac acgaacaaaa gacgcttcaa caaaacaacc tacagtcggg
    12241 ttcaatcctg actataatcc ttttgaaaag agacaagcgc ccttaacacc acggcaaatc
    12301 agcaaccgcg ataactggga caaaatgata cctcctaaag atttctcttt tggaaaagag
    12361 acggatagta ataccgattc tgtacctaag ctgatcgagg atgataatag cgatatggct
    12421 attcaactgc cggagagtaa ctttcttcag ttacatcgta agtatattat tacacaagtg
    12481 aagtcgggac tgatgatcat tgaccagcaa agagcacacg aacgaataat gtatgaacgt
    12541 tatcttgagc tactttccaa gcgagttacg atctctcagc aagtcctgtt tggagtcaaa
    12601 atatcgcttt cggcaggaga ttccgaactg ctcaacgaga ttatggaaga tttaaatctg
    12661 ctcggtttta atataaaagc tgataaaaat aaaccgtttc agtacattgc tgaaggaatc
    12721 ccagccggaa cagaaataga aaatgttcca gataccatag acagaatttt gcataactac
    12781 aaacaagggc taatagaatt cgctcagagc aaaagggtta atttagctcg ttcgatggcc
    12841 aaaaatttat cagtaaaaac cggtaaggtt ttgcagcagg ccgagatgaa aactattact
    12901 gaagaattgt tttcgtgtca ggcgcccgaa gtatcgcttg atggcgagcc gactgtgaaa
    12961 gtaataaata taaataaata ttttaatcac aaggaatgat agatattttg aacaataaat
    13021 tagaatccag gttgaatgaa taaactattt atcgtatgaa ttatcagcaa tatagtcctc
    13081 aaaggtttaa tgtattgcca cccgttgtaa agaacctttt aattatcaac gggttgtttt
    13141 ttttggctac cattgccttg cacaaatcct tcgggattga tttagcctcc tatcttgggc
    13201 ttcgctttcc gggttctcag gcctttaacc cttatcagtt tgttacctat atgttcatgc
    13261 acggcagcct ttctcatatt tttttcaata tgtttgcttt gtggatgttt ggttatacgc
    13321 ttgaaaatat ttgggggagc aaacgctttt taatctatta tttagttaca ggtatcggag
    13381 caggattggt tcactatggg acgctctatc ttgagtatcg cccgcttata ataaccttaa
    13441 atggtataat tgatgatcca tcggttgaga atcttgtaaa ttttagggca tctatacagg
    13501 gaacaatttc accctatgcc ggtgagattt ttctgaatta caataatttc atcaaagcgc
    13561 taaatcaatt aattcaaaag cctgaaagtc aagctttaat tgagagcgcc cgagggtttg
    13621 tgattgacta caaacagtat atgctcagcc gtcctaacgt cattggagct tcaggcgccg
    13681 tatttggcat attgctcgcc ttcggcatgc tcttccccaa tgtaaggctt tacctatatt
    13741 ttttctttcc gataaaagcc aaatggttcg ttattctcta tgggttatta gagctttatc
    13801 ttggtttcac acagtcgcat agtaatatcg cccattttgc tcacctcggc ggaatggttt
    13861 tcgggtttat tcttattaaa tattggaaac ataaacgcat atattaatca tgtttgccta
    13921 tcaaaaccct cttgacaaca ttaaaaaatt ttttaaaagc aaatcgcctt tagtttatct
    13981 catatatatt aatctggggg ttcttatttt agtacatctt actaatgtcg tttgctggtt
    14041 atttgaaatt aaaaaccatg ccggaacaaa tgctaatgta tcattactta catattacct
    14101 ctccctccca gccgatttag gacagatatc acttcgaccg tggacactat taacttatat
    14161 gtttctgcac gaaaacatcc tccacttcct gctcaatatg atagtgctgt attttggcgg
    14221 gaggattttt ttggaatatt taaccgaacg gaaattgttg agtgtatata ttttaggagg
    14281 cattgccggc gcattgttct atatcggggc ttttaatttt tttccggtct ttaaaggatc
    14341 aatttccgat tctatagcgt taggagcctc agcatcagtt ttggcaatac tttttgctat
    14401 tgcaacttat gttcctaatt atattatcgt tttattcatt ttcggaaggg taaagcttaa
    14461 gtatcttgct ttaatcattg tgcttgttga tatactaagc atacctttgg gaaatgccgg
    14521 cggccatatt gcacatctcg gaggcgcctt ctgggga
//
LOCUS       DAOOAI010000055        14533 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_5362_length_14533_cov_5.319450, whole genome shotgun sequence.
ACCESSION   DAOOAI010000055 DAOOAI010000000
VERSION     DAOOAI010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14533)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14533)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14533
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5362_length_14533_cov_5.319450"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(862..1587)
                     /gene="truB"
                     /locus_tag="PK345_05355"
     CDS             complement(862..1587)
                     /gene="truB"
                     /locus_tag="PK345_05355"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HPX34477.1"
                     /translation="MISKLSLTIIPNFEEGEVLLINKELDWTSFDVVNSIKAFLKYEL
                     GIKKIKIGHAGTLDPKATGLMIICTGKQTKSIEKYQSLDKEYTGSFTLGQTTPSWDTE
                     TTPNQNFPIDHITEKMIYDITNRFIGTILQRPPSYSAIWIDGRRAYKIARKDNKFKLE
                     PRQVEISEFEIVSVKIPQVNFRIRCSKGTYIRALANDFGKALNSGAYLSSLCRSAIGH
                     FKLEEALSINEFKERYKNPEEEW"
     gene            complement(1584..2375)
                     /locus_tag="PK345_05360"
     CDS             complement(1584..2375)
                     /locus_tag="PK345_05360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HPX34478.1"
                     /translation="MNWLEALILGIIQGLTEFLPVSSSGHLEIGKVILGVEAERSLIF
                     TVLVHGATVMSTIVIFWRDILLLIRGLFSFRWNEETKFIFKIIISMIPVAIVGLFFKE
                     EVELFFTGNLILVGSMLLLTATLLLFTRFKKSNERSIGFLDSFVIGLAQAVAVIPGIS
                     RSGSTISAGLMLGNKRAEVAKFSFLMVLIPIIGANLKDLLDGEFVSEGSIGIIPLIVG
                     FVAAFVTGLLACKWMIGIVKKGKLIYFSIYCYIVGSIAILSGIFT"
     gene            complement(2389..2706)
                     /locus_tag="PK345_05365"
     CDS             complement(2389..2706)
                     /locus_tag="PK345_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004329388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3098 domain-containing protein"
                     /protein_id="HPX34479.1"
                     /translation="MNKIETGVAKKSVKAEDTTKQQTNVDVNKDFLFEKENYRLLFIS
                     LGFIVVGFILMVGGRSDSTDFFNYEMFNFQRLTLSPILLIIGYALGIFAIMKKPKKSD
                     NQK"
     gene            2947..3618
                     /locus_tag="PK345_05370"
     CDS             2947..3618
                     /locus_tag="PK345_05370"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic pyrophosphatase"
                     /protein_id="HPX34480.1"
                     /translation="MSNKLMDPIGKLMGLRYKSHPWHGVDIGPDAPETITCFVEMVPT
                     DTVKYEIDKETGYLKIDRPQKFSNVIPALYGFIPQTFCADKVANYCMEKSGKKNIVGD
                     FDPLDICILTEKTIAHGDILVKARPIGGFRMIDGNQADDKIVAVLANDAVYGNYKSMS
                     DCSIIIIDRLKHYFLTYKVLPGSSRDAEITHTYDIEEAHEVIKHSMSDYQQKFDRLRT
                     MLSNV"
     gene            3686..6010
                     /gene="topA"
                     /locus_tag="PK345_05375"
     CDS             3686..6010
                     /gene="topA"
                     /locus_tag="PK345_05375"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006842975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HPX34481.1"
                     /translation="MAKNLVIVESPAKAKTIERFLGKDYIVKSSFGHIRDLSKKQLGV
                     DVANGFKPNYEIAEDKKNIIAELKKLANKADYVWLATDEDREGEAISWHLFETLKLDE
                     NKTKRIAFHEITKTAIDKALKNPRSIDIDLVNAQQARRVLDRLVGFELSPLLWRKVKP
                     ALSAGRVQSVAVRLIVEREEEIKTFNITPYYRITAEFDVQDSKKNSILRAEIPQRFKN
                     ETEARRFLEKCIGAEFTVSDIETKPFSKSPAPPFTTSTLQQEASRILGFRVTKTMQIA
                     QNLYESGWITYMRTDSVNLSDLALSMAKKEIVEHYGDNYHRSRKYSTKTKGAQEAHEA
                     IRPSYFERHTIEGTPDEQKLYSLIWKRAVASQMSDAKLERTTVNIAISTTPEYFVAKG
                     EVVLFDGFLKVYLDTSDDEDSEGKKGMLPPLKANQILDLIQMDATERFTQPPHRYSEA
                     TLVKKLEELGIGRPSTYAPIISTIQKRDYIIRDEGNGESRNYKLLTLKSQKIKESFKS
                     EKIGLTKGKLIPTDIGTVTTKFLLKHFNNIMDYQFTARVEKEFDEIAMGQKVWNKMID
                     SFYQPFHDHVTTTIEKGEKFSGERLIGIDPESGKNVFAKIGRFGPIIQIGSTESEEKP
                     RFAALKKGQSLETISLEEALDLFKLPRSLGNYEEEEMIVSIGRFGPYIRHKSKFYSIG
                     KTLDPFSIEANTAIDIIEKKRKQEREKIINVFPEEPLIQVLNGRWGPYISFNKKNYKI
                     PKNRDAKSLTLEECKEIIKKADTKSTKTAKPKKK"
     gene            6023..7201
                     /locus_tag="PK345_05380"
     CDS             6023..7201
                     /locus_tag="PK345_05380"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HPX34482.1"
                     /translation="MKASVFLEKFDAEKLGFISGVEDNKLLGNHIVQLQPYSMDDELR
                     SADIALIGVNEARHALNNEGCEMAPNQIRKYLYNLTPVHKNLKIVDLGNIKQGAHLSD
                     TYFALKAVVAELLLEDVIPIVIGGGQDLTYPIYLAFESLERIINLVAIDSRFNQVLNT
                     EEPDSQSYLSHIIMRNPSFLFNYANIGYQTHFVNQDDLKLLDNLFFDAYRLGSVRSDL
                     PEAEPILRNADMLTFDISAIRQSDAPGNGNASPNGFFGDEACQLMRYAGLAERLSCIG
                     FFETNPLLDHNGQTVHLVAQLIWYFLEGFTLRSNDFPTEKSEGFIKYTATQPGLNNDM
                     IFYKSKLTDRWWMQLPVSNDKADTLSRHVMIPCSYNDYLKAVKSEVPDRWWKAYQKLM
                     "
     gene            7242..8306
                     /gene="mnmA"
                     /locus_tag="PK345_05385"
     CDS             7242..8306
                     /gene="mnmA"
                     /locus_tag="PK345_05385"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004808 - tRNA
                     (5-methylaminomethyl-2-thiouridylate)-methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0002143 - tRNA wobble position uridine
                     thiolation [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HPX34483.1"
                     /translation="MKIASLVSGGVDSSVTIPLIKEMGYDPHLFYIEIGMKDDPNWID
                     CPAEEDIEIVTYIAKKYGCKMEIVSLQEDYWEKVISYTINAVKQGLTPNPDIMCNKLI
                     KFGAFNEKYGNSFDLITTGHYATILTIDHEKYLGTAKDTFKDQTYFLGQITKKQLDKL
                     MFPIGHLLKEEVRQKAIAEKLPSAYRKDSQGICFLGKINYSDFIERYVGEKPGKIIEL
                     ETKKVLGTHKGLWFHTIGQRKGLGLGQGPWFVVKKDIENNIIWVSNGYDPITQYKEII
                     ELADFHFLNEKPDRNYLKAQPIKFKIRHQPTFEDGTLINRGNSFVIHSKVPIAGVAPG
                     QYGVIYNTDETICLGSGVIC"
     gene            8385..8537
                     /locus_tag="PK345_05390"
     CDS             8385..8537
                     /locus_tag="PK345_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34484.1"
                     /translation="MITATIVDDEDGAIEVFKNMPQKIDEDDLSKMIQILKISPYKTN
                     EYPLSN"
     gene            8548..9483
                     /locus_tag="PK345_05395"
     CDS             8548..9483
                     /locus_tag="PK345_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34485.1"
                     /translation="MKSHFFHFSLVLFFTSLLFGCQEPDPIEITPDATPYAGKWTGNT
                     SQMTYIELDIRQINFKQMLYKATFNFWEDTTLKYRILTGEQGLCRIKRETIFVELPDH
                     GYIIGTFHNQNHLSGKIKVLSDENYKELYFDVTHADSSVTIHSLSRARFTARDTTWEY
                     NQVINDLFPISRVIQTDTVIIIETGIRIEKGMNIKRDVFLFKAGIFNTVEEVASFFVP
                     GHKQYAPMDKGGVEHIFFDPQLYLREWSSAWGSKSQQGSYFNIIEIQKIPHFEIEPMQ
                     RLKILVKFDCYVYNPKGDTLQISDGFFLGFVDLPL"
     gene            9593..12703
                     /locus_tag="PK345_05400"
     CDS             9593..12703
                     /locus_tag="PK345_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008823425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HPX34486.1"
                     /translation="MDYNFRYIEKKWQKYWADNKTFKVENQSDKPKYYVLDMFPYPSG
                     AGLHVGHPLGYIASDIYTRYKRLQGYNVLHPMGFDAFGLPAEQYAIQTGTHPQITTNQ
                     NIDRYRAQLNKIGFSYDWDREVQTCDPKYYKWTQWTFIQLFNHWYDIKTDKAQPISIL
                     VKHFEEHGSSSIEAICDEHPPFSSEEWKEMSEEKKNHILMNYRLAYLSDAMVNWCQEL
                     CTVLANDEVSEGLSIRGGHKIERKLMKQWSLRITAYAERLLNDLEKLDWSESLKDIQR
                     NWIGKSEGATLQFRIEDHKEKIEVFTTRPDTIYGATFMVLAPEHELALEITTPQNRGP
                     VKEYIEKVKMRSERERITEVKKVTGVFTGSFAINPLNQERMKIYISDYVLAGYGTGAI
                     MAVPAHDSRDFLFAKHFLLPIIQVVKRPDEEITNPQDWDDSYDSKEGIMINSGIISGL
                     EVKEAIDRITNYIQKLGVGKRKTNYKLRDAIFSRQRYWGEPFPIYYKNGIPYTLPESE
                     LPLMLPVVDKFLPTETGEPPLARAKNWKTKEGYPLETNTMPGFAGSNGYYLRFMDPHN
                     DNEYFSKEANDYWQNVDLYIGGAEHGTGHLIYSRFWNKFLFDLGYACKDEPFAKLINQ
                     GMIQGRSSFVYRVNLEKMAEYELWEELKDKKLGVTFLRDYKDGRRRFDFYSEEAKIII
                     EVKRQQSLEKIADPYEEYTNKKGFKLLLIPVSDFLNKDKVIERIKKTIRGEETSAFVD
                     KEPLGVFPVFVSKNYPGREHFSDAIHVDINLVNNDILDIEAFRKWQPHLANAKFILED
                     GKYICGWEVEKMSKSKYNVQNPDDLIEKYGADTLRLYEMFLGPIEQSKPWDTNGIEGV
                     FRFIRKLWRLFHDEHNNFRVTDEPPTSEELRVIHGTIKKVKEDIEQFSFNTGVSNFMI
                     CVNELHELKCHKRQVLEPLVKIVSPYAPHIAEELWQLLGYNESSANTSFPEYKDEYLI
                     QNTFEYPVSFNGKLRFKMIFPTDATISEIEKKILESEHTLKWIEGKKIKKVIVVPQRI
                     INVVVG"
     gene            12926..13819
                     /locus_tag="PK345_05405"
     CDS             12926..13819
                     /locus_tag="PK345_05405"
                     /inference="COORDINATES: protein motif:HMM:NF022750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HPX34487.1"
                     /translation="MPKHLNYKKQFSEFTTVIEMFPNNLIVMRPNCFLLLLALLINIS
                     FSHAQLCEDSVYRNIPNNAWVEGEHLKFKIYYESLLTGKLNAGTAQIEVKKTNRKFDN
                     REVFHIVGTGKSNRAFDLFFKVRDRFETYMDKKALISHHFIRRTKEGGYVRDDDITFN
                     HRKVTATSRKKTKTISPNIQDIISAVYYARTLSADTLKNGDYIYVNFYLDDSLYVSAV
                     QFQGRETIETELGIFNCLAFKPMVATGEVFSNPYPMTLWVTDDENKLPVLAKSAVIIG
                     SVKMELTNYSGLKNELRSKIK"
     gene            complement(13996..14397)
                     /locus_tag="PK345_05410"
     CDS             complement(13996..14397)
                     /locus_tag="PK345_05410"
                     /inference="COORDINATES: protein motif:HMM:NF014173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HPX34488.1"
                     /translation="MSKIFTLSEAGSIAIHSMVLIARSNDSLNVIKIAELTGSSKHHV
                     AKVLQRLAKEDFLDSNRGPHGGFRLKRSPDQISLLEIYEAIEGKIIVTDCPMDNSKCP
                     FDKCIMENTISTMATEFKNYLDTQKLSDCLE"
BASE COUNT         5018 a         2698 c         2624 g         4193 t
ORIGIN      
        1 ttatggaggc tactgcagca gcttacgacg aagtgataca catataaaaa gcaaaaacga
       61 aagctctgct caactcagtt gccagcagaa caagatgttt taaaaacatt cattattgaa
      121 atcaatcaga aaaaccttgt tgaatgatct ggttattgct gtataaagcc agcacatgat
      181 ttctacatcc attttattct cttttaacat gggttgttca ataaaaattc tattctattg
      241 cccacttagt gttctatgac atgtaagtac ataagtaaac gtaagcgtta gagcaaccga
      301 tgttaaagta tttagtaaaa tattgagctc tgtttatgat tgaccaggat aatctatcaa
      361 tctaattttt gttgtacaaa acgaaatcta ttgatgatac tgattttttc atctccttta
      421 cttcaataat atttcctttt gcaattagat gccgacttta cttaagcgcc tattaatatg
      481 atttgtaatc agcgccaata aaaaaaatga gaaaaatgtt tttggggcga tggtactttg
      541 gattttcata gggttttaag tttgggagcg ggggatatga atagaaatga aaaaagtgga
      601 ggtgttgaat atatccccct gtttttggtt aaactcccaa actacccctt aaaaccaata
      661 ctaaaaaaca aaaatattac aatccgcgca aatataaact cttttaagga gatacaacaa
      721 atttctttta cttttttgaa tacaaatacc taaatacaga gtaaaactta aaaatcctaa
      781 catgctttaa atcaaaattg aatttttaaa attattagtt caactcgaaa agagccttct
      841 attaacatac tcaatactaa cctaccactc ctcttcagga tttttatatc tctctttaaa
      901 ctcgtttatt gacagcgcct cttcaagttt aaaatggcct atggcgctac ggcacaacga
      961 agacaaatat gcgccgctat tgagagcttt tccaaaatca ttggcaagtg cacggatata
     1021 ggttccttta ctacaacgaa tacgaaaatt tacttgaggt atcttaaccg aaactatttc
     1081 aaattccgaa atttcgactt gtcggggttc taatttaaac ttattgtctt tccgtgctat
     1141 tttgtatgcc cttctaccat caatccaaat agctgaatat gatggcggtc gctgcaaaat
     1201 tgttccgata aacctattag tgatgtcata gatcatcttt tcggtgatat ggtcaattgg
     1261 aaaattttgg ttaggagtgg tctctgtatc ccacgatggc gtcgtttggc ctaaagtaaa
     1321 agagccggta tattctttat caagagattg atatttttcg atggactttg tctgtttgcc
     1381 ggtacaaata atcataagcc ctgtggcttt cggatcgagt gtgccggcat gaccaatttt
     1441 tattttttta attccaagct catatttcaa aaatgctttg atactattaa ccacatcaaa
     1501 agaggtccag tctagttctt tatttattaa taaaacttca ccctcttcaa aatttggaat
     1561 aatagtcaat gagagttttg aaatcatgtg aagatacctg ataaaatagc tattgagcca
     1621 acaatataac aatatatgga aaagtaaatc aacttaccct tttttacaat accaatcatc
     1681 catttgcacg ccagcaagcc ggtaacaaat gcagccacaa accctacaat caagggaatg
     1741 atgccaattg aaccttcgga cacaaattca ccatccaaca aatcttttaa gttagcgcca
     1801 ataattggga ttaaaaccat taggaacgaa aactttgcta cttcggcacg tttattaccc
     1861 aacatcaatc ctgcagaaat cgtactgccc gatcttgata tcccaggtat taccgccacg
     1921 gcctgagcta atccaataac aaatgaatca agaaaaccaa tagaacgttc gttagacttt
     1981 ttaaaacgag taaaaagcaa aagtgtagcg gttagtaata acattgagcc aaccaaaata
     2041 agattaccgg taaaaaaaag ctcgacctct tctttaaaaa acagacctac aatagctacg
     2101 gggatcatcg aaatgatgat cttaaaaata aatttcgtct cttcattcca ccgaaaagaa
     2161 aacaatcctc tgatcagcag taaaatgtcg cgccaaaaaa ttactattgt gctcatcact
     2221 gttgcaccgt gtactaacac agtaaaaatg agacttcgtt cagcttctac acctaagatc
     2281 actttaccaa tctcaaggtg accactacta ctaacaggaa gaaactcagt taatccctgt
     2341 attataccta atatcaacgc ttcaagccaa ttcattttgt aaagtagtct atttctgatt
     2401 atcagatttc tttggcttct tcataatagc aaagatgcca agcgcataac caatgataag
     2461 caaaataggt gagagcgtta acctttgaaa attaaacatt tcgtaattaa aaaaatcggt
     2521 gctgtcgcta cgaccaccta ccatcaaaat aaaaccaacc actatgaatc caagactaat
     2581 gaagagaagc cggtaatttt ctttttcaaa aagaaaatct ttattgacat caacattagt
     2641 ttgttgctta gtggtatctt ccgctttaac agattttttt gctactcctg tttcaatttt
     2701 attcatcctg ttaattattt atttttattc aaaatctggc tgtaaaagta gttaatataa
     2761 atcttctgaa tttattgtct gagatttttt aaaatcaaaa gatcagctca taggacattt
     2821 tttcaattaa tttggggttt atttttttta ttaccaatca atttgtccta tgatcatcta
     2881 cgtccatttt cggtatgagc agaatttatt gattattttc gcagcctttt tcaaatctca
     2941 cgacttatga gcaacaaact tatggaccct atcggaaaat tgatgggact acgatataaa
     3001 tcgcatcctt ggcatggtgt tgacataggg cctgatgcac ccgaaacgat aacatgcttt
     3061 gtagagatgg tacctaccga cacagtaaaa tatgaaattg acaaagaaac gggctacctt
     3121 aaaattgaca gacctcaaaa attctccaat gtaataccgg cgctttatgg ttttatacca
     3181 caaacatttt gtgccgacaa agtagccaac tattgtatgg aaaaaagcgg taaaaaaaat
     3241 attgttggag atttcgaccc tttagatatt tgcatcctaa cagaaaaaac tatcgcacat
     3301 ggcgatatcc ttgtaaaagc acggcctatt ggcggattta gaatgattga cggcaatcag
     3361 gcagacgata aaattgtagc ggtacttgct aatgatgctg tttacggcaa ttataaaagt
     3421 atgtccgact gttcgataat aattattgat aggttaaaac actattttct gacatacaaa
     3481 gttctccctg gcagctcacg agacgccgaa ataacacata cttacgatat tgaagaagca
     3541 catgaagtaa taaaacattc aatgagcgat tatcaacaga aatttgacag gctccgtacg
     3601 atgctttcca atgtttaaac cagatatcta aacactgact taaaattatt tttattaaat
     3661 taaaaccaaa attgaatttt tcattatggc aaaaaatctt gtaattgtcg agtcaccagc
     3721 aaaggcaaaa actattgaac gatttctcgg caaggattat attgtaaaat caagcttcgg
     3781 acatattcgc gacctctcaa aaaaacaact tggcgttgat gtagcaaacg gatttaaacc
     3841 caattatgaa atagctgaag ataaaaaaaa catcatcgcc gaactgaaaa agcttgccaa
     3901 caaagcagat tatgtatggc ttgccactga cgaagaccgt gagggggaag caatctcatg
     3961 gcacctcttc gaaacgttga aacttgatga aaacaaaaca aaaagaattg ctttccacga
     4021 aatcactaag accgctattg ataaggctct taaaaatccc cgctctattg atattgattt
     4081 agtgaatgcc caacaagccc gtagggtcct cgaccgttta gttgggtttg agctttcgcc
     4141 cttgctgtgg cgcaaagtaa aaccggcact ttcagcagga cgcgttcaat cggttgccgt
     4201 aagactaatt gtagaacggg aagaagagat aaaaacattc aatattactc cgtattatcg
     4261 cattacagcg gaatttgatg ttcaagattc caaaaaaaat tcaatccttc gtgcagaaat
     4321 accacagcgc tttaaaaatg aaaccgaagc aagaaggttt cttgagaaat gtattggcgc
     4381 tgaatttact gttagcgata tagagactaa gcctttttca aaatcgccgg cgccgccgtt
     4441 taccacctct accctccaac aggaggcaag ccggatttta ggctttagag ttactaaaac
     4501 gatgcaaata gctcaaaacc tttatgaatc agggtggata acttacatga gaaccgactc
     4561 ggtaaaccta tctgatctgg cgcttagcat ggcgaaaaaa gaaatcgttg aacattatgg
     4621 tgataattac caccgctcgc gcaaatattc tacaaaaaca aaaggcgccc aagaggcaca
     4681 cgaagctatc agaccctcct atttcgaacg tcacactatc gaaggaacgc cggatgaaca
     4741 aaaactatat agcctgattt ggaaaagagc cgtcgcatca caaatgagcg atgccaaact
     4801 cgaacgtacc actgttaata ttgctatttc caccacacca gaatattttg tagccaaagg
     4861 tgaagtggta ctttttgatg ggttccttaa agtctatctt gacacctccg acgacgaaga
     4921 ctcggaaggt aaaaaaggaa tgttaccacc actcaaagca aatcagatac ttgatttgat
     4981 tcagatggac gccaccgaac gtttcaccca gccgccacac cgttatagcg aagctacatt
     5041 agttaaaaaa ttagaagagc ttggcatcgg taggccgtca acatacgcac caataatctc
     5101 aaccattcaa aaaagagatt acattataag agatgaaggt aacggcgaat cacgcaatta
     5161 taaattattg acactgaaat ctcaaaaaat caaagagtcg tttaaaagtg aaaaaatagg
     5221 actaacaaaa ggaaaactta tcccaaccga cattggaaca gtaacaacaa aattccttct
     5281 caaacatttt aacaacatca tggactacca atttactgcc cgagttgaga aagagtttga
     5341 tgaaatagca atggggcaga aagtatggaa taaaatgata gatagttttt atcagccatt
     5401 tcatgatcat gttacgacta caattgaaaa aggtgaaaaa ttcagtggtg aacgattaat
     5461 tggaatagat cctgaatcgg ggaaaaatgt atttgctaaa attggtcgtt ttggtccaat
     5521 tattcagatt ggcagtactg aaagtgaaga aaaaccacgt ttcgctgcac ttaaaaaagg
     5581 acaatcttta gaaactattt cccttgaaga ggcgcttgat ctttttaaac tccctcgctc
     5641 cttaggtaat tacgaagaag aagagatgat agtttccata ggccgattcg ggccttacat
     5701 ccgtcataaa tcaaagtttt attcaatagg caaaacgctt gatccctttt cgatagaagc
     5761 taatacagca atagatatca tagagaaaaa gagaaaacaa gagagagaaa aaataatcaa
     5821 cgtttttcct gaagaacctt taatacaagt actaaatggc cgctgggggc catatatcag
     5881 ttttaacaag aaaaactata aaatacccaa aaacagagat gcaaaatcct taacattgga
     5941 agagtgtaaa gaaataatta aaaaagcaga caccaaatcc actaaaactg ctaaaccgaa
     6001 aaaaaaataa tcatgcccga tcatgaaagc ttctgttttt cttgaaaaat ttgatgccga
     6061 aaagcttggc tttatttctg gagtagaaga taataaattg cttggtaacc atattgtaca
     6121 gctacaaccc tacagtatgg atgatgagct aaggagcgct gatattgcgc ttattggtgt
     6181 aaatgaagcg cgacacgccc taaataatga gggctgcgaa atggcgccaa atcagataag
     6241 aaaatacctt tacaacttga cacctgtcca caaaaacctt aaaattgttg atctgggtaa
     6301 cattaaacaa ggcgcacatt tgagcgacac atattttgca ttaaaagccg tagttgctga
     6361 attattatta gaagatgtta ttcctattgt cattggtgga gggcaggatc tcacctatcc
     6421 tatatatctt gctttcgagt ctcttgaaag aataatcaat ttggtagcca ttgattctcg
     6481 ttttaatcaa gtattaaaca cggaagaacc ggattctcaa tcttatctat cacatataat
     6541 aatgcggaat ccaagttttt tatttaacta tgctaacatt ggatatcaaa cacattttgt
     6601 taatcaagat gatttgaagc tattggataa tctctttttt gatgcttaca ggcttggctc
     6661 tgtacgaagc gatcttcccg aggccgaacc gatccttcgt aatgctgaca tgctcacttt
     6721 cgacatttca gctataaggc aatcggatgc gcctggaaat ggtaatgcct cgcccaacgg
     6781 tttttttggt gatgaagcct gtcaattaat gagatatgca ggattagcag aaaggttatc
     6841 ctgtatcgga tttttcgaaa caaatcctct tttagatcat aacggacaaa cggtacattt
     6901 ggtagcacaa ctgatctggt atttcctcga aggtttcact ctacgctcca atgatttccc
     6961 aaccgaaaaa tccgaaggct ttattaaata tactgcaaca caacccggtt taaataatga
     7021 tatgattttt tataaaagta agcttaccga tcgttggtgg atgcaattgc ctgttagtaa
     7081 cgacaaagcc gacacattaa gccgacacgt gatgatacca tgtagttaca acgactatct
     7141 gaaagcagta aaatcagaag tgcctgaccg gtggtggaaa gcctatcaaa agttaatgta
     7201 acaacaagat gtcatatcat aaatcttcga acagagaata aatgaaaata gcatcattag
     7261 tttccggtgg tgttgatagt tcagtaacca ttcctctgat taaagaaatg ggttatgatc
     7321 ctcatctctt ttatatcgaa ataggtatga aagacgatcc taactggatt gattgcccgg
     7381 cagaagaaga tatcgaaatc gtaacttaca tcgctaagaa atatggctgc aagatggaga
     7441 tcgtctcttt acaagaagat tactgggaaa aggtaatctc ttacactata aacgctgtaa
     7501 aacaaggatt gaccccgaac cccgatatta tgtgtaataa actaatcaaa ttcggggctt
     7561 ttaacgaaaa atacggtaac agttttgatc tcattacaac aggacattat gccactatac
     7621 ttaccattga ccacgaaaag tatctcggca cagcaaaaga tacttttaaa gatcaaacct
     7681 actttctggg acaaattaca aaaaaacaac ttgataagct aatgttccct attggccatt
     7741 tgcttaaaga ggaggtacgg caaaaagcaa ttgccgaaaa actgccgtca gcctatcgca
     7801 aggatagcca ggggatttgt tttttaggaa aaattaatta ttccgatttc attgagcgtt
     7861 atgttggcga aaagccaggt aaaattattg aattggaaac aaaaaaagtc cttggaaccc
     7921 ataaagggct gtggtttcat accattggcc aacgcaaagg attgggactt ggtcaaggac
     7981 cgtggtttgt tgtaaaaaag gatatcgaaa ataatattat ctgggtttca aacggctacg
     8041 atccaattac tcaatataaa gagattattg aacttgctga ttttcacttt ttaaacgaaa
     8101 agcccgatcg caattatttg aaggcacaac ctataaaatt caaaataaga caccagccaa
     8161 cctttgaaga cggaacctta ataaatagag gtaattcatt tgtaatacat tcgaaagtac
     8221 ctattgcagg tgttgcgcca gggcagtacg gcgttattta caatactgat gaaacaattt
     8281 gtttaggaag cggcgttatt tgttagtatt gttacgaatt aataaaagct tccaataaaa
     8341 aaattagatt tgtacagatt tactcaccaa aagttattta aacaatgata actgcaacaa
     8401 ttgttgatga tgaagatggc gctattgaag tattcaaaaa tatgccacaa aaaattgatg
     8461 aagacgatct gagtaaaatg attcaaatac taaaaatatc tccttacaaa acaaacgaat
     8521 accctctttc caattaagcg ggacgagatg aaaagtcatt tttttcattt ttctttagtt
     8581 ttatttttta cctccttatt gtttgggtgt caggaacctg accctattga aattactcca
     8641 gacgctaccc cttatgctgg taaatggact ggtaacacat cccaaatgac ctacatagag
     8701 cttgatatca gacagatcaa ttttaagcaa atgctttata aggctacctt taacttttgg
     8761 gaagatacaa cactcaaata ccgcattttg acaggcgaac aaggtttgtg tcgaataaaa
     8821 agagaaacta tttttgtcga actccccgat catggataca ttattggaac ctttcataac
     8881 caaaaccatc tctccggaaa aatcaaagtt ctttcagacg aaaactataa agagctttat
     8941 tttgatgtta ctcatgccga tagctccgtt actatacact cactatcaag agcccgattt
     9001 acagcacgag atacaacatg ggaatataat caggtaatta atgacctttt cccgatttcc
     9061 agagtcatcc aaacagatac agtcataata atcgagactg ggataaggat tgaaaaagga
     9121 atgaatatca aaagagacgt ttttctgttc aaagccggaa tatttaacac tgttgaagag
     9181 gttgcatcat tttttgttcc cggacataag caatatgctc caatggataa aggtggtgta
     9241 gaacacatat ttttcgaccc tcagctatac ttgagagagt ggtcgtcagc ctggggaagc
     9301 aaaagtcaac aaggaagtta ttttaacatc atcgaaattc aaaaaatacc tcatttcgaa
     9361 attgaaccaa tgcagcgatt gaaaatactt gtaaaatttg attgttacgt ttacaatcct
     9421 aaaggtgata cattacaaat aagtgatggg ttttttcttg gattcgtaga tttacctttg
     9481 taaaaaatca tcaaatcatt aaagataaaa acaacaagca ttatgttaaa tactctctct
     9541 taagagtttt ttttaatttc gcacgctgat aataggataa tgatcaattg aaatggatta
     9601 caattttaga tatattgaaa aaaaatggca gaaatattgg gcagataaca agactttcaa
     9661 agttgaaaat cagtctgata agccaaagta ctatgtgctc gatatgtttc cttatccttc
     9721 cggagcagga ttgcatgtag gccatccact cggttatatc gcatcagata tttatacacg
     9781 ttataagcgt ttgcaaggat acaatgtatt acacccgatg ggtttcgatg ctttcggact
     9841 tcccgccgaa cagtatgcca ttcaaacggg tactcatccg caaataacga caaaccaaaa
     9901 catagatcgt tatcgggcgc aattgaataa aataggattt tcgtatgact gggatcgaga
     9961 ggtgcaaacc tgtgatccaa aatattacaa atggacacaa tggactttta tacagctttt
    10021 taatcattgg tatgacatta aaaccgacaa agcccaacct atcagcatat tggttaaaca
    10081 ttttgaagaa catggcagct cttcaataga agcaatatgt gatgaacatc ctcctttcag
    10141 tagtgaagaa tggaaagaaa tgagtgaaga aaaaaagaat cacatattga tgaactaccg
    10201 acttgcatat ctttctgacg caatggtaaa ttggtgtcag gaattatgta ctgtgcttgc
    10261 taatgatgag gtgagtgaag gcctttcaat aaggggaggg cataaaattg aacgaaaact
    10321 gatgaagcaa tggtcgctaa gaattacagc ttatgccgaa cgattgctca atgacctcga
    10381 aaagttggat tggtcggaat cattaaaaga tatacagcgc aactggattg gaaaatcaga
    10441 aggggcaacc ttgcaattta gaattgagga tcataaagag aagatcgaag tttttacaac
    10501 acgccccgat accatctatg gcgctacctt tatggtttta gcgccagaac atgaattagc
    10561 tttagaaatt acaacacctc aaaaccgtgg gccggttaaa gagtacatcg aaaaggtaaa
    10621 aatgcggtcg gaacgtgaac gtataacgga ggtaaaaaaa gtaacaggag tgtttacggg
    10681 ttcgtttgct attaatcctc tcaaccaaga gcgaatgaaa atttacatct ccgactatgt
    10741 ccttgcaggc tatggtacag gggccattat ggccgttccc gctcacgata gccgcgattt
    10801 tttatttgcc aaacactttt tacttccaat tattcaagta gtaaaacgtc cggacgaaga
    10861 aataacaaac ccacaagatt gggatgactc ttacgactcg aaagagggta taatgatcaa
    10921 ctccggcata atatcgggac tggaagtaaa agaggctatt gatcgaatta ccaattacat
    10981 tcaaaaattg ggtgtcggta aaagaaaaac aaactacaaa ctcagagatg caatctttag
    11041 ccgtcagcgt tactggggcg agccttttcc gatatattac aaaaatggta ttccttacac
    11101 actacccgaa agcgaattac cgcttatgtt accggtagtt gataaattcc ttccgaccga
    11161 aacgggtgaa ccgcctttgg caagggctaa aaactggaaa accaaagagg gatatcctct
    11221 cgaaaccaat acaatgccag gatttgccgg atccaatggc tattaccttc gctttatgga
    11281 cccacacaac gataacgaat atttttccaa agaagcgaat gactattggc aaaatgttga
    11341 cctatacata ggcggtgcag aacacggtac cggacatttg atctattcac gcttttggaa
    11401 taaattcctt ttcgaccttg gatatgcttg taaagacgaa ccttttgcga aactgatcaa
    11461 tcagggaatg atacaaggca gatcgagctt tgtatataga gtaaacctag aaaaaatggc
    11521 agagtacgaa ctttgggaag agttaaaaga taaaaaactt ggggttactt ttttacgtga
    11581 ttacaaagat ggacgtcgta ggtttgactt ctatagtgaa gaagcaaaga ttatcattga
    11641 agtaaaaaga caacaatctt tagaaaagat tgccgatcct tacgaagaat atactaataa
    11701 gaaagggttt aaactccttt taattcccgt tagtgatttt cttaataaag acaaagtgat
    11761 cgaaagaatt aaaaagacaa ttcgcggaga agaaacaagc gcctttgttg ataaggaacc
    11821 tcttggtgtt tttcctgttt ttgtatcaaa aaattatccg ggcagggaac acttttctga
    11881 tgccatccat gtagatatta atctggtaaa taatgacata ctcgacattg aggcctttcg
    11941 taaatggcag ccacatcttg ccaatgctaa gttcatcctc gaagatggta aatatatttg
    12001 tggatgggaa gtggaaaaga tgtcaaaatc aaaatataac gttcaaaatc ccgatgatct
    12061 gatagagaaa tatggagctg ataccctacg gctctacgaa atgttcctcg gaccaataga
    12121 acagtcaaaa ccatgggata ccaacggaat tgaaggtgtc ttcaggttta ttcgaaaact
    12181 atggaggctt tttcatgatg aacacaataa cttcagggtt accgatgaac caccaacatc
    12241 tgaagaactt agagttatcc atggaactat taaaaaagtt aaagaagata tagagcagtt
    12301 ttcgtttaat accggagtca gcaactttat gatatgcgtt aatgagttac acgaactcaa
    12361 atgtcataaa cggcaagtac tcgaaccatt agtaaaaatc gtttcgccat acgctccaca
    12421 tattgccgaa gaactttggc agttgttagg gtataatgaa agcagcgcaa atacatcatt
    12481 tcctgaatac aaagatgaat acctgataca aaacactttt gagtatccgg tttcgttcaa
    12541 tggcaaatta cgctttaaaa tgatatttcc cacagatgct actatatcgg aaattgagaa
    12601 aaagatatta gagtcggaac atactctaaa atggattgag ggaaaaaaga taaaaaaagt
    12661 tatcgttgtg ccccaaagga taatcaatgt agtagtagga taaagatcat taaattcaaa
    12721 ccaagcgctt gtaatcaata aaagaataat tgccttgaat aaacactagt tcgggattgc
    12781 tctcgtaaaa tcaatagtat tgcctaaaaa cgccttttta ttgattgatc ggcttaaaag
    12841 tgttttcaaa ctatatttct ctttctaatt ttattttttt cggtctggtt tgaaaattgt
    12901 agttacgaca caaactactt caaccatgcc aaagcatctt aactataaaa aacaattttc
    12961 cgaatttact acggttattg agatgtttcc taataacctt atagttatga ggccgaactg
    13021 ttttttgttg cttttggcgc ttcttattaa catttcattt tctcatgctc aactttgtga
    13081 agattctgta tatcgaaaca tacctaacaa tgcatgggtt gaaggtgaac atctaaaatt
    13141 taagatctac tacgaatcat tgctcacagg caaattaaat gctggaacag ctcagattga
    13201 agttaaaaaa actaatcgca aatttgataa tcgggaggtt tttcatatcg tgggtaccgg
    13261 aaagtccaac cgtgctttcg acttgttttt taaagttcgc gatcgttttg agacatacat
    13321 ggacaaaaag gcgcttatat cacaccactt catacgccgg acaaaagaag gcggatatgt
    13381 aagagacgac gatatcacct ttaaccatag aaaagttaca gcaacaagcc gtaaaaaaac
    13441 taaaacaata tcacccaaca tccaagatat aatttcggca gtatattatg cacgtacatt
    13501 aagcgctgat acactgaaaa atggcgatta tatctatgtt aatttttatc tcgatgattc
    13561 tctgtacgtt tcagcagtac agtttcaggg tagagagacg attgaaaccg aacttgggat
    13621 atttaattgt cttgctttca aacctatggt agccaccggc gaggtgttca gcaaccccta
    13681 tcctatgaca ctatgggtta ccgacgacga aaacaaattg cccgttttag caaaatcagc
    13741 agtgataatc ggttctgtta aaatggaact aaccaactac tcgggattaa aaaatgaatt
    13801 gagatcaaaa atcaaataag aaaaaaaact taacaacaat tattaaaaag ccttggggat
    13861 aataagttta ccaccggtaa tgaagtatgt gcgaatcaaa tctttctccc ttgttcttga
    13921 aaagttattt cttttacttt aatcaacatt ctttcaatta aaaaaacaat ttatcaacca
    13981 attatctaat aaggtttatt caaggcaatc cgacaacttt tgagtatcga gatagttttt
    14041 aaattctgta gccatcgtgg agatagtgtt ttccataatg catttatcaa atggacattt
    14101 cgaattatcc atcggacaat ctgttacaat tatctttcct tcaatagctt cataaatttc
    14161 aagtaacgat atctgatcag gtgatcgttt taatcgaaaa cccccatgcg gacctcggtt
    14221 ggagtctaag aaatcttctt ttgccaaacg ttgtaatact ttagctacat gatgttttga
    14281 tgaccctgtg agttcagcaa ttttgataac gttaaggctg tcatttgatc tggcaatcaa
    14341 taccatacta tgaattgcaa ttgacccagc ttcggaaaga gtaaatattt tcgacattaa
    14401 attgtacagt taaaaaaaat caaatcctga cttgtccctt ttagtgcggt aaaaataatt
    14461 tttcatagct tttcgctttt ggacttaaat taactctcat tgtgacttct tttccggtta
    14521 tttggtttaa gat
//
LOCUS       DAOOAI010000056        14393 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_5424_length_14393_cov_5.293207, whole genome shotgun sequence.
ACCESSION   DAOOAI010000056 DAOOAI010000000
VERSION     DAOOAI010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14393)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14393)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14393
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5424_length_14393_cov_5.293207"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            58..264
                     /locus_tag="PK345_05415"
     CDS             58..264
                     /locus_tag="PK345_05415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34489.1"
                     /translation="MKFKKRKTTYENTYQTGRPLTDEEYNYRRKEKQERIDEILEKIA
                     RSGYSSLTKEEKALLFQSSNKNNS"
     gene            264..1394
                     /locus_tag="PK345_05420"
     CDS             264..1394
                     /locus_tag="PK345_05420"
                     /inference="COORDINATES: protein motif:HMM:NF015338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPX34490.1"
                     /translation="MRRIRIFRTGIKMLLLIVNICFLVMLLISYCAPIYSPDVFFFPA
                     IIGLFYPILLIINFAFVIFWLIQLKIYFLLSLLAIIAGWNIMKKNIGTNKNKYPEELQ
                     SGFKYLSYNVRLFDQFKWVKGQDYFTRDAIFKFIASESPDVLSFQEFFHGDENYFPTI
                     GPFIKMQLTDYYHVDYSKVAGKQKHYGLATFSRFPIVNKGSIRFANSTSNSGIYTDIV
                     INSDTIRVFNFHLESIRLSNADYNYVAEFIDPSIQSQRNSSSDIILGKFKKAFIKRAE
                     QARIAAEYIQKSPYPVIVSGDFNDTPISYSYHLISKGLDDAFLKAGRGLGTTYAGKIP
                     FIRIDYILNSKKLTSSNFTIHKVSYSDHYPISCIFWLNNKSD"
     gene            complement(1984..3228)
                     /locus_tag="PK345_05425"
     CDS             complement(1984..3228)
                     /locus_tag="PK345_05425"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPX34491.1"
                     /translation="MLPFQKKLTNLFYVVLGLPATAMGFGLSVQIAVLSWILRTNYGL
                     DIHEIGIVWAAGPLAGIIGQPIIGLISDEVWFWGGRRRPFIIIGGVFAALSILALPYI
                     GLIGQALGIGSIIAVAITVALTLDLAINISLNPTRAIIADVTPEGEPRTKGYTWMQAI
                     SNLFGAGAYLISALLGNFTLIFIGAAIVFLFSVVPMFFIAEPRKLEATETGEVKASAT
                     EWGELLKIYIAHAFSWVGIQTMFIYSFAFLEQRFNATDPTKLGSIIGWSFFILNGAGA
                     IFPILLLEPLAKKIGKVKTQVLAVASMSLGYLLIFLFVRSTAMMYALFVLLSIGWSAV
                     VSLPFAIMTEKVNKKRMGFFMGIFNLSVVLPQLISSFVLGSVIQNADNKSVVYFISGI
                     TLAVSAFLWAWVKEKKSRSLKL"
     gene            complement(3260..5503)
                     /locus_tag="PK345_05430"
     CDS             complement(3260..5503)
                     /locus_tag="PK345_05430"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HPX34492.1"
                     /translation="MIFFTWMALFFIRINPLLAQPNFPDDGEVFRDDVVPKIDIYIHP
                     DTLQWIYNNPHSNIEWRASFVFDNGNIHDSIVEVGFRLRGNTSRQSAKKSFKVSFNTY
                     QKGRKYYGLEKMNLNGEHNDPSVTRAKLCWDLARSLEIPAPRSNHVRVFINGNYFGLY
                     LNVEHIDDEFVQSRYGTQYGNLYKCLYPADLKYLGTNPNLYKYESGGRRAYDLKTNRA
                     YDDYTDLSEFIQILNNTPIGLLPCELERVFNVQDYIKVLAFDVFIANWDGYIYNKNNF
                     YLYKNPESGRFEYIPYDLDNTFGVDWANIDWANRNIYYWAPSNQQSEPRPLYTRLMSV
                     QRYKDLFSFYLNKIAQEILIQPDYYTYMDDIRDRIYPYIIDDPYYPLDYGFNTDTFLE
                     AYDNAWGNHVKYGIKPYFTTRLNSLQQQIQLNPIYPVINYIQVEHGGVGSPVNFSLTV
                     WDDQPEVQVWLRYRFNQSEFVTMPMTPLQNDKFEATLDGVLGQVTLRYHFQVKDASGN
                     FIFYPCETEELYFAPLYTSGLYINEFMASNASTIFDENGEFDDWIELYNAGPEAIWLG
                     DKYLSDKLNNPTKWQMPDHAIFPGDYLIIWADDDMEQGPFHAGFKLSADGESIGIFNN
                     EQNNYSAIDTCTFSSQQTDVSFGRSPDGNSMWVFFDEPTPGSSNTVYTINDNMNSASG
                     FFVYPNPAKSNIIHLPNKKSFYIYDTSGRLLIEGKGKDFIDVSTLDRGVYLLKTFEND
                     IVKIIIQ"
     gene            complement(5574..7439)
                     /locus_tag="PK345_05435"
     CDS             complement(5574..7439)
                     /locus_tag="PK345_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HPX34493.1"
                     /translation="MLRVKLVNPLFLFCLLILFSVADLMSQDFGFDRVEPPFWWVGMK
                     NEDLQLLVYGKNISLTEPRISDGAMTIQRVIKADSPHYLFLDIKISLDAAPGLYPIVF
                     YKDDKQLFEYDFELKMRRSGSALRKGIDQSDVIYLLFPDRFVNGNPNNDSHPEMLEKA
                     DKSNPDGRHGGDLAGMINKLDYIENLGFTAIWHNPLLENNQERYSYHGYAISNFYRID
                     PRFGNNDDYLLFVKLSHEKGIKVIMDMIFNHCGSGHWWMNDLPWDDWINQHESFTRSN
                     FRAGTVFDPYASDWDRDLFQKGWFDTNMPDLNQSNPYLMNYLIQNSIWWVEYADIDGI
                     RMDTYPYPDKDGMALWAERLMGEYPNLSVIAEAWLTEPAQVATWQRGDKSGIGYESSV
                     SHVFDFPLYEAFRFAFNEEAGWSTGMVRFYNLLSQDFVYPNPGNIVVFADNHDGSRIF
                     TKVNENLNSLKLAMILVLTTRGVPQIYYGTEIMMAGDETKGHGDVRKPFPGGWSTDVR
                     NAFTKEGRTHEENEIFDFMHLLLNWRKNKLVVHFGELRHFIPENDVYIYFRYNETEKI
                     MILLNNNQSEQLIDGRRFAELTDGFSKGVNIFDGKEFLLDKIKIPSKTGLILELR"
     gene            7906..8562
                     /locus_tag="PK345_05440"
     CDS             7906..8562
                     /locus_tag="PK345_05440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34494.1"
                     /translation="MNSNRKYWIVITLSVINSLVVLFLVIRHWSGNNLFEKDNYGRRQ
                     RDSIFGKAYSNPENGLNDFYLNNNYLETLFYADSLYKAGELSKAREIYFYADTLYAFY
                     NYPLMKIKLIDSILSSRQSHILYQESYNKQVNLEQTLSAMKYHIIVGVFREASMASNV
                     MQRVKDSGKMPIVRPYKDKNLIVVIHSSYVSFGEAELELPAVRKEIVPDAWILHTTFN
                     "
     gene            8619..10163
                     /locus_tag="PK345_05445"
     CDS             8619..10163
                     /locus_tag="PK345_05445"
                     /inference="COORDINATES: protein motif:HMM:NF024500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OstA-like protein"
                     /protein_id="HPX34495.1"
                     /translation="MPKFKKFFLIFKLFFILLSLLSNHYDLFSQKKTTINLIKAEKMI
                     GRQTPDEELNIFTGAVLFEHDSVLLYCDSAVLRNKANTLDAYNNIRIKINDTLNLYGN
                     LLFYDGNTRLATVIDDVKLVDNDATLFTDKLWFNRNSGIGYYNTNGRIINNKNELVSR
                     EGYFHTNTKILYFKKNVVLTNPDYRLESDTLVYNTSTEIAYISGPTTIRGKDEFMYAE
                     EGWYETKNGKTMLKTNPYVIDGERYIAGDTIFYEKDIDAGDVFGNVFLKDTIQKLNVQ
                     GDYCKYRRGEGYAFVTQKPWATFVEKNDSLWLHADTLRLYFDSIGQADKFTAYNHCKF
                     FKKDIQGKCDSLTYNLNDSTIIMYERPVVWTQDTQITSELIKIFTSHNSIDSMYIFNS
                     AFIISMDETDPNSFNQVKGKNMKAYFTENELYKINVLGNSETIYFVREEDGSLIGINR
                     AFSANMEIFVADRQITDIFYFDKPDATLFPEEEIQPNELRLRDFKWHDAERPKTSEDI
                     FLWDNK"
     gene            10247..11125
                     /gene="rfbD"
                     /locus_tag="PK345_05450"
     CDS             10247..11125
                     /gene="rfbD"
                     /locus_tag="PK345_05450"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005634066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HPX34496.1"
                     /translation="MQNILITGADGQLGSVLKSLAANYPEFKFYYTDIAELNITNQAA
                     IDDFVSGHNITCILNTAGYTNADKAESDSEQAFLINGTAVGFLAESAKKNNALLIHIS
                     TDYVFGNNHFRPIVESASPYPLSVYAASKVDGEEKAIEHNPNTVILRTSWLHSKFGHN
                     FVKTIIKLGNERDQLRVVFDQIGSPTYAVDLAQTILMLIPIWNKISAPEIYHYSNEGV
                     ASWYDFAVATLDLAGINCKIQPIETKDYPLPAARPHYSLLNKEKIKTKYGITIPHWQE
                     SLKKCIAEITKENHAN"
     gene            11320..13065
                     /locus_tag="PK345_05455"
     CDS             11320..13065
                     /locus_tag="PK345_05455"
                     /EC_number="5.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular transferase
                     activity, phosphotransferases [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HPX34497.1"
                     /translation="MEKKKIDPKVLAIATSWLEGNYDSQTKAAVKDMIDNNPDELTDS
                     FYRNLEFGTGGLRGIIGAGTNRMNKYTVGMATQGLANYIKKAMPGEKEYRAAIAFDNR
                     LKSDYFAQITADVLSANGFKVFLFDELRPTPELSFAVRYLKCHTGVMITASHNPKEYN
                     GYKAYWNDGAQMVPPHDKNVIAEVEKIKIEDVKFKGVKKNIELIGDEIDEAYLSEIDK
                     LSLSPVSNKRQAELKIVYTPLHGAGVKLVPAALKRYGFENVFRVNKQDINDPTFPTLH
                     SPNPEEAAALEMALDKARKVDADLVLATDPDADRVGVAIKDLNDNFILLNGNQTAAII
                     TYYLLTLWNKKGKLTGNEFIVKTIVTSELICDIAKKYKVECYDVLTGFKWIAEIIRNN
                     EGKKTFIGGGEESYGFMIGDFVRDKDAVATCVMMAEISAWATNLGKTLFELLIDIYYE
                     FGFYKENLISITKKGKAGAEEIQKMMENFRNNPPKEINKSNVMMIKDYLAGKAKDLVN
                     NTESEIKLPSSNVLQFFTTDGTKISMRPSGTEPKIKFYFGVKGILKNKNNYEELNKQL
                     EEKIENVIKALKLRD"
     gene            complement(13153..13479)
                     /locus_tag="PK345_05460"
     CDS             complement(13153..13479)
                     /locus_tag="PK345_05460"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPX34498.1"
                     /translation="MRILTLVSFMVFVLFWSACSKDKDDKKEVKAAFSWELIEEPGKV
                     LFTNLSSNAVSYEWNFDDGTSSTVASPTKVYNRNGEYIVTLKAFGEKKIHSVYDTVTV
                     DNIVTR"
     gene            13589..14080
                     /locus_tag="PK345_05465"
     CDS             13589..14080
                     /locus_tag="PK345_05465"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPX34499.1"
                     /translation="MKITKNIVVTIHYTLTDDNSEVIDSSQGGEPLTYLHGHGGIIPG
                     LEAELEGKMLDDAFKVSIQPDLAYGVIDKNLIFRINRSSFADSKNIKTGMQVRGQLTK
                     EGPSMIFWVKEVNNEDIVLDGNHPLAGQTLHFDVKVIELRNATKEEIAHGHVHGPHGH
                     HSH"
     gene            14241..14324
                     /locus_tag="PK345_05470"
     tRNA            14241..14324
                     /locus_tag="PK345_05470"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:14275..14277,aa:Leu,seq:cag)
BASE COUNT         4802 a         2850 c         2578 g         4163 t
ORIGIN      
        1 atttttatta ataaaacaga acactaatag cgacttaaaa aaataaattt tggccatttg
       61 aagttcaaaa aaagaaagac aacttacgaa aatacttatc aaaccggcag accgctcaca
      121 gatgaagagt acaactatag gcgaaaagaa aaacaggaaa ggattgatga gattttggaa
      181 aaaattgctc gctcagggta tagcagcctt actaaagagg agaaagccct gctgtttcag
      241 tcgagcaaca aaaataatag ctaatgaggc gtataaggat atttagaacc ggaataaaga
      301 tgctcttgtt gattgtcaac atctgctttc ttgtaatgct ccttatatcc tattgtgctc
      361 ctatctatag ccctgatgtt ttcttttttc cggcaataat cggccttttt tatccaatat
      421 tgttaataat caactttgcc tttgtcatat tttggcttat tcagttaaaa atctatttcc
      481 tgctatcgtt gcttgccata attgccggtt ggaacattat gaagaaaaac atcgggacaa
      541 ataaaaataa atatcctgaa gaactacaat caggatttaa atatctatcg tataatgttc
      601 gacttttcga tcagttcaaa tgggtaaaag ggcaggatta ttttacaagg gatgcaatat
      661 tcaaatttat tgcttccgaa agccctgatg ttctaagctt tcaggaattt tttcatggcg
      721 atgaaaacta tttccccaca atcgggccgt ttattaaaat gcaactaacc gactattacc
      781 atgttgatta ttcaaaagtt gccggcaaac agaaacatta tggtctggca acctttagcc
      841 gctttccgat tgtgaacaaa gggtcaataa gatttgccaa ttctacctca aactccggta
      901 tttataccga tattgtaatc aatagcgaca cgatcagggt ctttaatttt catcttgaat
      961 ccatcaggtt atctaatgct gattataact acgttgctga gtttattgac ccgagtattc
     1021 aatcacaaag gaattcaagc tccgatataa tacttggtaa atttaaaaag gcttttatca
     1081 aaagagcgga gcaggcccga attgcagccg aatatatcca aaaatcaccc tatcctgtga
     1141 tcgtctccgg cgactttaac gataccccta tctcatacag ttatcacctt atttcaaagg
     1201 ggcttgatga tgcatttctt aaagccggac gagggttggg aactacatac gcaggcaaaa
     1261 taccttttat ccgaattgat tacattctga acagcaaaaa attaacttct tcgaatttta
     1321 ctatacacaa agtctcctac tccgatcact atccaatatc atgtattttc tggctaaaca
     1381 ataaaagcga ttagtaaaaa cctcattgac ccgaattgct atttaatata taaggagtga
     1441 tcttaaatat atttaattct ggtatcatat cctgaataaa gtctgttttt aatattcttt
     1501 tgtttgatat cggcaagttc ttacggaaaa attttaaata aagcgctttt tatatctccc
     1561 ctctattttt ataaatagcc caagcctttt tcaattttaa aagtgtttcc ttaaaattat
     1621 ttttatcaag tcaattcggg atgatcctaa accggttagc ttaactttga aaacattatt
     1681 cttgacaccc ctcctaaaaa atggttttta tcgcttttca atttacatgt ccaactgttc
     1741 acatttttaa acatgatata cacctccggc agtgcatttc gatactttta aagcaatacg
     1801 ctcctccccc attgctttag cgaagttgga gatgcttctg catttatacg accccaaatc
     1861 gcatcttgtt aaaaattttc aggatactac cttttcgata tccgactatt ttgccctacc
     1921 tctcttacta ctttgaaatt attgttggtt tttcttatat aagtaattaa caaaaacaat
     1981 aaattacaac tttagtgatc tagatttttt ctcctttacc caagcccaca gaaaagccga
     2041 tacagcaagg gttataccgc tgataaaata gacaacgctt ttattgtcgg cattttgaat
     2101 aacgcttcca agtacaaagc tggaaattaa ttgaggtaaa accaccgata ggttaaatat
     2161 acccataaaa aatcccattc gttttttgtt tactttttca gtcattattg caaaaggcaa
     2221 gcttactact gctgaccatc ctatactaag cagtacaaat agcgcataca tcattgccgt
     2281 tgacctgacg aacaaaaaaa tcaatagata acctaacgac atagaggcta cagcaagaac
     2341 ctgtgttttt accttaccta tcttttttgc gagtggttcc agtaagagga ttgggaatat
     2401 agcgcctgcg ccgttaagaa taaaaaacga ccatccgata atactaccaa gttttgtcgg
     2461 gtcggtagca ttaaaacgct gttctaaaaa tgcaaaagag taaatgaaca ttgtctgaat
     2521 acctacccac gaaaatgcat gtgcaatata tatctttaaa agctcgcccc attcagttgc
     2581 agaagcttta acctcacctg tctcagtcgc ttcaagcttt ctgggttcag cgataaaaaa
     2641 catagggaca accgaaaaga gaaagactat tgcagctcct atgaagatca aagtaaaatt
     2701 cccgagtaat gcacttataa gataggcgcc tgctccaaat aagttagata tggcctgcat
     2761 ccaggtatat ccttttgttc gtggttcacc ttcaggggtt acatctgcaa ttattgcacg
     2821 tgtagggttt aaacttatat taatagccaa atcaagagta agggcgacag taatagcaac
     2881 ggctattata ctgccgatac ccagcgcctg accaataagg ccaatatatg gcagcgctaa
     2941 aattgaaagg gcagcaaaca cgccgccaat aattatgaaa ggtcgtcttc tgccgcccca
     3001 aaaccatacc tcatcgctta ttagaccaat tatcggctga ccaataatac cggcaagagg
     3061 gcctgcagcc catacaatac ctatttcatg aatatcaagc ccgtagtttg tccgcagaat
     3121 ccaacttaaa acggcaatct gcactgaaag cccaaaaccc atagctgttg caggtaaacc
     3181 taaaacaaca taaaacaagt ttgttaattt tttttgaaaa ggaagcatac ttgacaaatt
     3241 aagtttttaa atcaattaat tattgtataa ttattttaac tatatcattt tcaaaagttt
     3301 ttaacaaata gacacctcta tcaagagtcg agacatcaat aaaatcttta cccttaccct
     3361 caattaataa tctacccgaa gtgtcgtata tataaaagct ttttttatta ggaaggtgta
     3421 ttatatttga cttcgccgga ttaggatata caaaaaaacc agaggcgcta ttcatattat
     3481 catttatcgt ataaacagta ttggaagagc cgggcgtggg ttcatcaaaa aagacccaca
     3541 tagagttacc atcaggactg cgaccaaaag aaacgtcagt ttgttgtgag ctaaaagtac
     3601 atgtatcaat cgcagaataa ttgttctgtt cattattgaa aatacctatt gactcgccat
     3661 ctgctgaaag cttaaaacct gcatgaaaag gaccttgttc catgtcgtca tcagcccaaa
     3721 taataagata gtctcctgga aaaatggcat gatcaggcat ctgccatttt gttggattat
     3781 tcagcttgtc agataaatat ttatcgccta accatatagc ttccggcccg gcattataaa
     3841 gttctatcca atcatcaaat tctccattct catcaaaaat tgttgaagca tttgaagcca
     3901 tgaactcatt aatatatagc cctgatgtat aaagtggggc aaagtataat tcttctgttt
     3961 cgcaaggata aaaaataaaa tttccagagg catcctttac ttggaaatga tatctgagag
     4021 ttacctggcc aagtactcca tcaagggttg cctcaaactt atcattctgc aatggcgtca
     4081 taggcatagt aacaaattcc gactgattga atctatatct aagccatact tgcacctctg
     4141 gctgatcgtc ccacactgtc aggctaaaat taacagggct gccaacgccg ccatgctcaa
     4201 cctgtatata attaattacc ggatatatcg gatttagttg gatctgctgc tgcaaagagt
     4261 ttaacctcgt cgtaaaatag ggtttaattc catacttgac gtgattcccc catgcgttgt
     4321 cgtatgcttc aagaaaagtg tctgtattga aaccatagtc aagcggataa taaggatcgt
     4381 caatgatgta aggatatatc ctatccctga tgtcatccat ataagtataa taatcaggtt
     4441 gaatgagtat ctcctgtgct attttgttca gataaaatga aaataggtct ttataacgtt
     4501 gcaccgacat cagccttgtg tatagaggcc gaggctccga ctgctgattg gatggcgccc
     4561 aataatagat attacgattt gcccaatcaa tattagccca atccacgcca aaggtattgt
     4621 caagatcata cggtatatat tcaaaccggc cggattccgg gtttttataa agataaaagt
     4681 tgtttttatt ataaatgtat ccatcccaat ttgcaataaa gacatcaaac gcaaggactt
     4741 taatataatc ctgaacatta aaaacccgtt caagctcaca aggcagcaat cctatcggtg
     4801 tattgttgag tatctgtata aactcgctca agtcggtata atcatcataa gcacgatttg
     4861 ttttcaggtc ataggcacgc ctgccgccac tttcgtactt atataggtta gggttagttc
     4921 ccaaatactt gaggtcggca ggatagaggc atttataaag atttccgtat tgagtgccat
     4981 atctggattg tacaaactca tcgtcaatat gctcgacatt aaggtatagc ccaaaataat
     5041 ttccattaat aaataccctt acatgattgg aacgcggcgc aggtatttca agagaacgag
     5101 ccaagtccca gcacaattta gcacgggtaa cagagggatc gttatgctcg ccattgagat
     5161 tcatcttttc aagtccataa tactttcttc ctttctgata agtattaaat gatactttaa
     5221 aagacttttt tgccgattgg cgagaagtgt tacccctaag cctaaaccca acctcaacaa
     5281 tgctatcgtg gatattgcca ttatcaaaaa caaaagaggc gcgccattcg atattgctgt
     5341 gcggattgtt gtaaatccat tggagtgtgt caggatgaat gtaaatatca atttttggaa
     5401 cgacatcatc acgaaaaact tctccgtcat caggaaaatt aggctgagcc agtaaagggt
     5461 tgattctaat gaaaaacagc gccatccagg taaaaaaaat catcctgaca ggacttatat
     5521 gcaaaaaggc tatgtttgtt ttaaagcaca ttaagtcaat agattttcaa ttattaacgc
     5581 aattccaata tcaatcctgt ttttgacgga atctttattt tatcaagtaa aaactcttta
     5641 ccatcaaaaa tattgacccc tttactaaag ccgtcagtaa gttcagcaaa tctacggccg
     5701 tcaatcagtt gttctgattg gttattattg agcaggatca ttattttttc ggtttcgttg
     5761 tatctaaaat agatgtaaac atcgttttcc ggtataaaat gcctcaactc tccaaaatgt
     5821 actactaact tgttctttct ccagttgagt agaagatgca taaagtcaaa aatttcgttt
     5881 tcctcatgcg ttcttccctc tttagtgaag gcattccgaa catctgttga ccatccccca
     5941 gggaatggtt tcctcacatc gccatgtcct ttggtttcat ctccggccat cataatttca
     6001 gtaccgtaat atatctgggg aactccacgt gttgtcaata ctaaaatcat tgcaagttta
     6061 agactgttca aattctcatt taccttagta aaaattctgg agccatcatg gttatcggca
     6121 aatacaacga tattcccagg attcggataa acaaaatcct ggctaagcaa attataaaac
     6181 ctaaccatcc ctgtactcca accggcttct tcattaaaag caaatcgaaa tgcctcataa
     6241 agcgggaaat cgaaaacatg gctaactgat gattcgtagc ctatcccgga tttatcgcct
     6301 ctttgccaag ttgccacctg cgccggctcg gtaagccatg cttcagcaat tacagacaaa
     6361 ttgggatatt cgcccatcaa tcgctcagcc cataatgcca tcccatcttt atcaggatag
     6421 ggataagtat ccatcctaat accatcaata tcggcatatt caacccacca gatactgttc
     6481 tgaatcaggt aattcatcaa ataggggttt gactgattca gatcaggcat atttgtgtcg
     6541 aaccaacctt tttgaaaaag gtctctgtcc caatccgatg cataaggatc aaatacagtg
     6601 ccagccctaa agttcgatcg tgtaaaactc tcatgctgat ttatccagtc atcccatggc
     6661 aggtcattca tccaccaatg tccggagcca caatggttaa agatcatgtc cataataact
     6721 ttaatcccct tttcgtgcga aagcttgaca aataaaagat aatcgtcatt attgccaaaa
     6781 cgcgggtcta tcctataaaa gttgctgata gcatagccat gatatgaata tctttcctga
     6841 ttattttcga gaagcgggtt atgccaaata gcagtaaagc caaggttctc aatataatcc
     6901 aatttattta tcatacctgc caggtcgcct ccgtgtctgc catcaggatt cgatttatca
     6961 gctttttcca acatttcagg atgcgaatca ttattcgggt taccattgac aaaacgatca
     7021 gggaaaagta aatagataac atcgctctgg tcaatgcctt tgcgaagcgc cgagccggag
     7081 cgccgcattt ttaattcaaa atcatattcg aatagttgct tatcatcttt gtaaaatact
     7141 attggataga ggcccggcgc ggcatcaagg gagattttta tatcaagaaa aagataatgt
     7201 gggctgtcag ctttaataac gcgttgaatg gtcattgctc catctgaaat ccgaggttcg
     7261 gtcagtgaaa tgtttttgcc ataaactaaa agctgtaaat cttcattttt catacccacc
     7321 caccaaaaag gcggttctac acggtcgaag ccaaaatcct gcgacattaa atcggcaacc
     7381 gaaaacaaaa tcaacaaaca gaataagaaa agaggattaa ctaatttgac tcgtaacatt
     7441 ttgatattta atattataaa attaaatccg tcctatttga aatattatca cagatgaatt
     7501 tttactaaca atcaccattt cttatactaa tcgaaacaaa agataatttt tatctaaaat
     7561 taaaacttca aaagtaaata atattaaaaa aagcctttta agtagtatta tcccaaattg
     7621 agcatttagc aaaacccgaa cgattattta ttcataatca gaacaatagc gcttttaata
     7681 ataagcggca attcgatatt ttttcttcct aaaaggtaac aattggtaag attttataat
     7741 tttagcaaaa atttgatcta actttttttt taaaaaatat gtcaataaaa aatacttaaa
     7801 cttttaattg gttcattata ataccgaact tactgtagtt tgtacatttt tttaaatatg
     7861 aagtttcaat aaatgtctta aaaaggataa gctaacctct ttatgatgaa tagcaacaga
     7921 aaatattgga ttgtaattac cctttcggtg atcaattcgc ttgtggtact ttttcttgtt
     7981 atcaggcatt ggtcgggtaa taatctcttt gaaaaagata actatggacg ccgacaacgt
     8041 gattcaattt ttggaaaagc ttacagcaat cctgaaaatg gcctcaatga tttttatctg
     8101 aataacaatt acttagagac attattttat gccgatagtc tttataaagc cggagagttg
     8161 tcgaaggcca gagaaatata tttttatgcc gacacacttt atgcttttta caattatccg
     8221 ctaatgaaaa tcaaattgat tgactctatc ctctcctctc ggcaaagcca catcctttat
     8281 caggaaagtt acaacaaaca agttaatctt gaacaaacgc tctcggccat gaagtaccat
     8341 attattgtag gagtttttcg tgaagcaagt atggcctcca acgtaatgca gagggttaaa
     8401 gatagtggaa aaatgcctat tgtgagacca tataaagata aaaacctgat tgttgttatc
     8461 catagctcat atgtcagttt cggagaagcg gagcttgaac tgccggccgt tagaaaagag
     8521 attgttccag atgcatggat tttacataca acatttaatt gataatgcta aatccgccaa
     8581 tacaaccgtt taagagccat tcgatgcaga tcagcgaaat gccaaaattt aaaaagtttt
     8641 tcttaatttt taagttgttt tttatcctgt tatctttatt atcaaatcat tatgatttgt
     8701 tttcgcagaa aaaaacgacc attaatctca tcaaagccga aaaaatgata ggtcgtcaga
     8761 cacctgatga ggagttaaac atctttaccg gcgctgtgct tttcgagcac gacagtgtat
     8821 tgctttattg cgatagcgct gtacttcgta acaaggccaa cacattggat gcatacaaca
     8881 acatacgcat taagatcaac gacacgctga acttgtatgg gaatttgctt ttttacgatg
     8941 gcaacacccg attagctacc gtgattgatg atgtgaaact tgtggataac gatgcaacgc
     9001 tttttaccga caagctctgg ttcaaccgca atagcggaat agggtattac aatacaaacg
     9061 gaaggattat aaacaacaaa aatgaacttg taagcaggga gggatatttt cataccaaca
     9121 ccaaaattct ctatttcaaa aaaaatgtag tattaaccaa tcctgactat cgtcttgaat
     9181 ccgacacgct cgtttataat acgtcaacgg aaatagcata tatatcaggg ccaaccacga
     9241 taaggggtaa agatgaattc atgtatgccg aagagggctg gtatgagaca aagaatggca
     9301 aaacgatgtt gaaaacaaat ccttacgtca ttgatggtga gcgatacata gctggcgata
     9361 caatttttta tgaaaaggat atagatgccg gtgatgtttt tggcaatgta tttctgaagg
     9421 acaccattca aaagctgaac gtacaagggg actattgcaa ataccggcgt ggcgaagggt
     9481 atgcttttgt tacccaaaag ccgtgggcaa cgtttgtcga aaagaacgac agcctatggc
     9541 tgcacgccga cacgttgcga ctatacttcg actccatagg tcaggccgat aaatttacag
     9601 cctacaacca ttgcaaattt tttaaaaaag atattcaagg caaatgcgac tcgcttactt
     9661 ataacctaaa cgactcaaca attatcatgt acgaacgtcc ggtagtatgg actcaagata
     9721 cgcaaataac atcggaactg atcaaaatat ttacttcaca caactcgata gactcgatgt
     9781 atatattcaa cagcgctttt atcatctcga tggatgaaac cgaccccaat agctttaatc
     9841 aggtaaaagg taaaaatatg aaagcctatt ttaccgaaaa tgagttatat aaaataaatg
     9901 tgcttggaaa tagcgaaacc atctactttg ttcgtgaaga ggacggaagt cttatcggga
     9961 ttaacagggc attttcagca aacatggaga tatttgtggc tgatcgccag atcaccgaca
    10021 tcttttactt cgacaaaccg gatgcaaccc tttttcccga agaagagata caacccaacg
    10081 agctaagatt aagggatttc aaatggcacg atgcagaaag accaaaaacg agcgaagata
    10141 tttttttatg ggataataaa taaaacagag agcctttaaa tgaagttatg actaagacaa
    10201 acaatgatcg gttatatttt taaaatactt tttaacaaaa accactatgc aaaatattct
    10261 gataaccggc gccgacggac aacttggtag tgtgctaaaa tctttagctg caaattaccc
    10321 tgagtttaag ttttattata cagatattgc cgaactcaat atcaccaatc aggcagctat
    10381 agatgatttc gtgtcgggcc ataatattac atgtatcctc aatacagcgg gttatactaa
    10441 tgctgataag gcggagagtg actccgaaca agcttttctg ataaatggta ctgcagtcgg
    10501 ctttttagct gaatcagcca agaaaaacaa tgctttactg atccacatat ctaccgatta
    10561 cgtttttggc aacaaccatt ttcgcccgat cgttgaatct gcatcacctt atccactctc
    10621 ggtatatgcg gcatcaaaag ttgatggtga agaaaaggct atcgaacaca accctaacac
    10681 cgttatacta cgcacctcgt ggcttcactc aaaatttggc cataatttcg ttaaaacgat
    10741 aattaagctt ggcaatgaac gagatcagct cagggtagtg tttgatcaga tcggatcgcc
    10801 aacgtatgct gttgaccttg cccaaacaat acttatgcta atacccattt ggaataaaat
    10861 cagcgctccg gagatatatc attactcaaa cgaaggcgtt gcaagctggt acgattttgc
    10921 agtggctact ctcgacctgg caggtatcaa ttgcaaaata caaccgatcg aaacaaaaga
    10981 ttatccgctg cctgccgctc gcccgcacta cagcctgttg aacaaagaaa agattaaaac
    11041 aaaatacgga ataacgatcc cgcactggca ggagagttta aaaaaatgta tcgcagaaat
    11101 aacaaaagag aatcacgcta attgacgtta gacataatta gttgattaag aaaatattat
    11161 gatcaacccc tctctttttc tcattgcaaa catgaaattg gctaattttg caagattcga
    11221 ttaaaagata aaatcatttt tttgaaactg atgagatact tgaataagac tttaaaaaca
    11281 tcattttttt aaaacggaaa tcattaaata ttcataattg tggaaaagaa aaaaattgat
    11341 cctaaagtat tagctattgc cacaagttgg cttgaaggca actacgacag ccagaccaag
    11401 gcggcggtaa aagatatgat tgacaacaat cctgatgagc ttaccgattc cttttatcga
    11461 aaccttgagt ttggcaccgg tgggctacgc ggcataatcg gcgcaggcac caaccggatg
    11521 aacaaataca cggtgggcat ggctactcaa gggcttgcca actatataaa aaaggctatg
    11581 ccaggcgaaa aagagtacag agccgctata gcgttcgaca accgcctgaa gagcgattat
    11641 tttgcacaaa ttacagccga tgtattgtca gccaatggtt ttaaagtctt cttgttcgat
    11701 gagcttaggc ctactccaga gctatctttt gcagtgcgtt atctgaaatg ccatacagga
    11761 gtgatgatca ccgcctcgca taatcccaaa gaatacaacg gttacaaagc ctattggaac
    11821 gatggcgcac aaatggtgcc accgcatgat aagaatgtga ttgccgaagt ggaaaaaatc
    11881 aaaatagagg atgttaaatt caaaggggta aagaaaaata tcgagttgat aggagatgaa
    11941 attgacgaag cctacctaag cgagatagac aagctatcgc tttcgcccgt ttcgaacaag
    12001 cgtcaggcgg agcttaaaat tgtttacaca ccgcttcatg gcgccggtgt aaaacttgtt
    12061 cctgccgcct taaagcgcta cggattcgaa aatgttttta gagtcaataa acaagatata
    12121 aatgacccaa ccttccctac attacattcg cccaatcctg aagaggcagc agcccttgaa
    12181 atggccctcg acaaagcccg taaagtagat gccgacttag tattagccac cgaccccgat
    12241 gccgacagag tgggagtagc cataaaagac cttaatgata attttatact gctcaacgga
    12301 aaccaaacgg ctgcgattat aacctattac ctgcttacat tatggaataa aaaaggaaaa
    12361 ctaacaggca atgagtttat agtgaaaaca atagtaacat ccgaattgat atgcgatata
    12421 gcaaaaaaat ataaagtcga atgttacgac gtactaacag gattcaaatg gattgccgaa
    12481 atcattcgta ataatgaagg aaaaaaaacc tttatcggag gtggtgaaga gagttacggc
    12541 ttcatgattg gcgattttgt aagagataaa gatgcagtgg ctacatgcgt aatgatggcc
    12601 gaaatttcag catgggcaac aaacttaggc aaaacactat ttgaattgct tattgatatt
    12661 tattatgagt tcggctttta caaagagaac ttgatctcga taaccaaaaa aggaaaagcg
    12721 ggagctgaag agatacaaaa aatgatggag aattttagaa acaatcctcc aaaagagatc
    12781 aataaaagca acgtgatgat gattaaagat tacctcgccg gaaaagctaa agaccttgta
    12841 aataataccg aatccgaaat taaactaccc tcctccaacg tgcttcaatt ttttaccacc
    12901 gatggcacaa aaataagcat gagaccctcg ggtaccgaac cgaaaattaa attctacttc
    12961 ggagttaaag gcatattgaa gaataaaaat aattatgaag agctgaacaa acagttagaa
    13021 gagaaaatcg agaatgtcat taaggctctt aagttgagag attaatagcc gcaaaaataa
    13081 tctattgagc ttcagctttt aacggtaaac tatgcattaa aatccctacc tgcttcaaag
    13141 aaaataatac ttttagcggg taacaatatt atcaaccgta acagtatcgt aaaccgagtg
    13201 gattttcttt tcgccgaacg ctttcaatgt aactatatac tcaccattgc gattataaac
    13261 ttttgtgggg cttgcaacag tacttgaagt gccatcgtca aaattccatt cataacttac
    13321 ggcattgctg ctaagattag taaacagcac tttgccaggc tcttcaataa gttcccaact
    13381 aaaggctgct tttacctctt ttttatcatc tttgtcctta ctacatgccg accaaaataa
    13441 aacaaaaacc atgaaactaa ctaatgttaa tattctcata acttttatta tttgattttc
    13501 gagcgacaaa attattcaaa attgaattgc cctgcaataa aagttaaaac ttatcgtcaa
    13561 tgggagatca atatataaac tgctaaagat gaaaattaca aaaaacattg ttgttactat
    13621 ccattataca cttactgacg ataactctga agtaatagac tcatcacagg gcggtgagcc
    13681 tctcacctat cttcatgggc atggcggaat catacccggt cttgaagccg agcttgaagg
    13741 aaagatgtta gatgatgctt ttaaagtttc cattcaacct gatttagctt atggtgtaat
    13801 tgacaaaaat ttgatttttc ggataaatcg ctccagcttc gccgatagta aaaatattaa
    13861 aacaggaatg caagtacgcg ggcaactaac taaggagggg ccttcgatga tattttgggt
    13921 aaaagaggtt aataatgaag atatcgttct tgatggtaat cacccattag cagggcaaac
    13981 cttacatttc gatgtaaaag ttattgagct acgaaatgcc acaaaagaag aaatagctca
    14041 cggccacgtg catggaccac acgggcatca cagccattaa tatagtatag aacatttaca
    14101 atacgaaatt attatggttt tttttaaaac cagtatctca acacaaaaac gcctcctatt
    14161 gattaagtct ggctaaaatt atatggcgct tttgcacaaa acaaaaaata accattattt
    14221 ttgcggctac aaataaaaat gcccaggtgg cggaattggt agacgcgcta gtttcaggga
    14281 ctagttcccg aaagggagtg caggttcgat tcctgttctg ggcacagaaa aagtcttgca
    14341 ataaaatcat ttgcagggct ttttttatac ctattaatta atggcttatc gca
//
LOCUS       DAOOAI010000057        14307 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_5481_length_14307_cov_5.879596, whole genome shotgun sequence.
ACCESSION   DAOOAI010000057 DAOOAI010000000
VERSION     DAOOAI010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14307)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14307)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14307
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5481_length_14307_cov_5.879596"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2768
                     /locus_tag="PK345_05475"
     CDS             <1..2768
                     /locus_tag="PK345_05475"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPX34500.1"
                     /translation="FGPNGQGYIPATPAELGGWTGGTAQMVVNAINSGAFALQHRDHG
                     YEQGWGEPDFISSHINSLTNNQNNELVYVFSINCLTGKYNMSTECFTEKFHRHTYGGQ
                     NAGALGLIAASEVSYSFVNDAFVWGMIDNMYPEFMPDYGNNIPMRGFLPAFGQAAGKI
                     FLQSSQWPYNSSNKEVTYNLFHAHGGAFLQVFTEVPQNLAVTHNPILYSSESSFSVIA
                     PAGAFISLTVNGEIIGTAMSNGGSTNIAIEPQLPPNTMVVTVTKQNYYRYESNVDVIP
                     PTGPYVVGDSYVINDASGNNNGLMDYGESVMLHMTMKNVGVAVANNVSTTMTSNNEYV
                     TITNGNAYFGNIAPNGTVTVNNAFSLTVSPNIPDNHAVSFTLNSTDGTDNWISYISIV
                     GHAPNIKFKQYVVNDEGGNGNGLLDPGETAPMIITVENQGSSAAYNVVGMLTTLDPYV
                     TILTSQPQNMGNINAGGTSNCTFTVSANANIPAGYTADLTLNLNADLGVTQQTTIQLL
                     FPDYCYPTASCSWGDGFTGFALEQINNMTGNGCSPNGYGDYTNMVADLEGGQTYSVQW
                     RTGYSNQQASLWIDLNDNKEFEPSERLITDFVLSSSNQIYTTTFTVPAGSYTGNKRIR
                     IRANWQNSSSDPCANFTYGETEDYTANFGSQTVLAPPQNVSANVTGNSVVVTWQAPAG
                     ADATLLGYNVYRDNQKISNTITSLTYTDTNVPTGSHWYSVCAVYTEGESELAQPVQVN
                     VGSLQGKLQGFIRNAVTNAVIQNAWVSALDSDFGAVTYNTIFGSHYTLYLSAGTYSVS
                     CTAPGFQTLTVNNVVILEGQTKALNFYLHSSSESNLGGEVTGVSYFEFEDFSVYPNPA
                     SSEINITTTSAMKHVKIMNNMGQVVFVDGGGDLLIRVNTTGFSRGIYFIEVETENHRF
                     FEKLIIN"
     gene            complement(2844..4235)
                     /locus_tag="PK345_05480"
     CDS             complement(2844..4235)
                     /locus_tag="PK345_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009596543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HPX34501.1"
                     /translation="MKVKYIDLIEQTFEFPQEEFNVVDGQLYFHNIPLMDIIKQYGTP
                     VKLTYLPKISSQIQKARRLFNVAMAKVDYHGEYKYCYCTKSSHFLFVLEEALKNKINL
                     ETSSAFDIHIIEELYDIGLITKDIIIVSNGFKRVQYLENIIRLINNGFENTIPILDNM
                     DELNYYVDNLDKNFNVGIRIASEEEPMFNFYTSRLGIRYNDIVPFYHEKIKNNPKIGL
                     KMLHFFINTGIKDTAYFWNELNKSINVYVELKKVCPELDSFNIGGGFPIKRSLGFEYD
                     YEYMAEEIIAQIKSVCEQHGVPEPDIFTEFGSYTVGESGAVLYSVLDQKQQNDRELWN
                     MIDSSFMTTLPDSWAINQRFILLPVNRWEADYQRVFLGGLTCDSQDYYNDEAHTNAVF
                     LPKFDKNDTLYIGFFHTGAYQESLGGYGGIQHCLIPAPKHIIIDLDENGDYFPKLFAK
                     EQSYKSMLKTLGF"
     gene            complement(4796..5383)
                     /gene="gldD"
                     /locus_tag="PK345_05485"
     CDS             complement(4796..5383)
                     /gene="gldD"
                     /locus_tag="PK345_05485"
                     /inference="COORDINATES: protein motif:HMM:TIGR03512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldD"
                     /protein_id="HPX34502.1"
                     /translation="MTKGYLFAIGLIMIIMLISSCENQPMLPRPAGYFRIDLPEHKYQ
                     PFDSTFPYYFKYSQSARIQFDNYTSDDPFWLNIYYPKYKARIHLSYKSLKDNKLFNLT
                     EDARNFVFQHAEKAQGIKENTIKINESKMFGLVYRIEGKEVASPFQFWVSDSSTHFLR
                     GALYFSVRPNNDSLEPVINFIVKDIDHLLSTLEWR"
     gene            complement(5392..6747)
                     /gene="gldE"
                     /locus_tag="PK345_05490"
     CDS             complement(5392..6747)
                     /gene="gldE"
                     /locus_tag="PK345_05490"
                     /inference="COORDINATES: protein motif:HMM:TIGR03520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility-associated protein GldE"
                     /protein_id="HPX34503.1"
                     /translation="MDDPDIYLNLKPLFIASSIILHPISPQVIIGLIIIAFLVFCSAF
                     ISASETAFFSLTPSNLRELRTDKIKNSEKLIKLIEQPKRLLATILIANNFVNVAIVIL
                     SSFLLLSIVELKDNPVIEFLIQVVLITSIILIFGEIIPKIYSVSKALKIALLVTPVFK
                     YLIKFLSPFSALLVSSSGIIDKRLIRKSAQLSLGDLSQAIDLTATESTPAEEHKILKG
                     IVKFGDIEAKEIMKPRIDVVAVDVLTTYHKLLDIILNSGYSRIPAYNENFDKIAGILY
                     VKDLLPHIDKPDDFDWASLLRPAFFVPENKKINFLLKEFQSKKIHLAIVVDEYGGTSG
                     IVTLEDIIEEIVGDISDEFDTFEDELSYSKIDDYTYLFEGKTLLNDFYKITGIEDTIF
                     EDIEGDPDTLAGLIIEIERRIPKLNNITKFKQYEFKITKVDSRRVLEVQVTLPKPGSV
                     Q"
     gene            complement(6912..7343)
                     /gene="ssb"
                     /locus_tag="PK345_05495"
     CDS             complement(6912..7343)
                     /gene="ssb"
                     /locus_tag="PK345_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013446417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HPX34504.1"
                     /translation="MAGINKVILVGNLGNDPNVLTFENGVKKATFSLATNETYKDKEG
                     NRVEQTEWHNIVVWRGLAEVAEKFLRKGSLIYLEGKIKTRSYEQNGIKKYITEIFGDT
                     FTMLGKMESSGSSQYQPPIVPDKDDSDLPPPEHAPTDDLPF"
     gene            7585..9114
                     /locus_tag="PK345_05500"
     CDS             7585..9114
                     /locus_tag="PK345_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HPX34505.1"
                     /translation="MLTFLFSCNQPTHNKLPRIAIAGIAIESSTFSPAVTYEEAFFGK
                     IADEVFSYYPFLDEDSAVRKRAVWFPTFRGHAMPGGIVAREAYESMTEKILIMLKEAL
                     PLDGLLLDIHGAMSVVGLDDPEGDLIVRIREVIGKDVVISTTMDLHGSVSERLAENTD
                     LITCYRLAPHDDVIETKKRAVVNLLDRLESGKGKPAYKVHIVVPILLPGEKTSTRIEP
                     AKSLYSQIDPLTKKPGIIDAAIWMSYPWADEPRNHGVVMATGDDKEAVASVAEKLASD
                     FWNARNDFVFVAPTTTLDECLEKAINSEVKPFIISDMGDNPTAGGAGDVTWTITKILE
                     NPTFYKQGGKRIIYASLPDEKFTEEALALGEGASIKAFAGARVDNRYAPPILLDGTIK
                     KIMEGDSRAKVEVVVEVGNVDVIVTQGRKAYHYEEDFTRLGLNPRETDILVIKIGYLA
                     SELYEMRADWIMALTPGGVDQDLERLEYKRVQRPIFPLDKNMADPDLSARFIISSDDV
                     K"
     gene            9532..10410
                     /locus_tag="PK345_05505"
     CDS             9532..10410
                     /locus_tag="PK345_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34506.1"
                     /translation="MKFLKFFAFALLISSFAIFTGCSKDDEDVDLGPTISLKTGEGYT
                     SSDFEVFNDSTVLFGIVANKSTTHNNLLSKLNIYYNELTLLDSTLNTANFSGDFEIKF
                     IGIGTGKLRFKITAEGGLTDETSLNVTILESPVVGVEVDKNSGVELGSFNDPVGSFYS
                     TTERKAYKVAEAMLEQGKIDFLFFKGVTNFNTIAAPDDEDAATISDFHLTEWTTKNPT
                     RFYLADITAEDFDAIEDKCVFPEFDAEKAETKANELKNNDVIFFKTVGDKLGYIKIVD
                     LYHRGDRIKFDVIVMK"
     gene            10571..11710
                     /locus_tag="PK345_05510"
     CDS             10571..11710
                     /locus_tag="PK345_05510"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPX34507.1"
                     /translation="MIDALFESLLQQQQFSLFFYTFVLLLFIQLIFFLVCFNRLNSRK
                     LQKEIDVKQSVSVVLVTSNQYNDLRKLLPEILNQSYSNYEVVVVDDSSEDGSDVLLER
                     FSEQYSHLKVVNMHQHLNWFKGNEFPISIGIKSAVNDWILLTETTCIPRSSHWISEMM
                     KKANFPTQIVLARSSFATSSKINIWYRFARFYNSLINLSFAISKISFKGSTQNLLFSR
                     KLFFDQKGFVSHYTIKGGAGELFVSEAATLSNVSVQISPKSVVDYVKSTSFRKWIIKE
                     HTNLDIERRINLKQRIPFLLYFNSLFIFYLVFGILMINNLHWQVILSLFSIRLVSQFF
                     IFHRAQKRLSEQRLLLLMPVFEIALLVIHFLIRFRLIFIKKGVWV"
     gene            11701..12888
                     /locus_tag="PK345_05515"
     CDS             11701..12888
                     /locus_tag="PK345_05515"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HPX34508.1"
                     /translation="MGLGKTKYIFDEVISRKGTDCVKYDLRKLYFGTEDVIPMWVADM
                     DFRTPDFIIEAIRRRLDHEILGYSIRSEEYSSAISNWMLVRHNWQINPAWISFSPGVV
                     PALNMLVLAFTEPGDKIIVQPPVYFPFFAAIENNGRKKVENPLRLIEGRLSMDFDDLR
                     KKAQGAKMILISHPHNPGGSVWTKEELTSLADICLENNVLMISDEIHSDLIFSPHKHI
                     PLASLSKKVSNQTITCNAPSKTFNLAGLSTSYLIIPDKAKLNVYNSILNDKLHLNMGN
                     IAGSVALQAAYNFGADWLKQLLEYVAKNVELVCVFCNEYLPQITPIIPESTYLIWLDC
                     RKTGMKGDDLKDFFIKKARVGFNDGRLFGTGGEGFMRINVACPRSVVLEALQRIKNAF
                     QYL"
     gene            12982..>14307
                     /locus_tag="PK345_05520"
     CDS             12982..>14307
                     /locus_tag="PK345_05520"
                     /inference="COORDINATES: protein motif:HMM:NF027900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5060 domain-containing protein"
                     /protein_id="HPX34509.1"
                     /translation="MKHQLQFVLLYCIFCLALNNKANAIAPIIHSVIPNNTTIARYEK
                     FELIVDVTATYASPYDFDQVNLQATFTSPSGNVFVVDGFYYQDFTMTQPNVLTPNGAP
                     DWRIRFSPNQKGVWSYTVKITDTQGTASFPSQQFTCITSLHKGFVKRSGNNLVYDNGD
                     RFLAIGTNLAWTEWASGFTIYDEWITSLKSYGANYVKITMAPWIFGLEWGSGAMGNYA
                     GRQNRAWALDWVFDKLEQKNIYCQLHFLVHDELRTGVNPGWDDNPYKNTNGGPCANPQ
                     DFFTNSTAKKYFKQKLRYSNARWGYSSYLHSWEIMSEVDNVSFYNSNYSQIFNWLLEI
                     TNYVKSKDVYSRAVSSGYAWPQNDSAYWNNSAVGYSQLHIYDFISDLEMKLYNYSRHY
                     IEKYQKPNIISEFALGHEPNNIFQNDPEGVAFHNVLWSSVFSGTMGTAMS"
BASE COUNT         4461 a         2439 c         2720 g         4687 t
ORIGIN      
        1 attttggccc taacggacaa ggatatatcc cggccacacc tgctgaatta ggcggttgga
       61 caggtggtac tgctcaaatg gttgttaatg caattaattc tggcgctttt gcgcttcaac
      121 atcgtgatca tgggtatgaa caaggatggg gagagcctga ttttatcagt agtcatatca
      181 atagcttaac aaataatcaa aataacgagt tagtttacgt gttttctatc aactgtttga
      241 cagggaaata taatatgtca accgaatgtt tcactgagaa attccatcgt catacttatg
      301 gcgggcaaaa tgcaggagct ttgggattaa ttgccgcctc tgaagtatct tattcttttg
      361 ttaatgatgc ttttgtttgg gggatgatag ataatatgta tcctgaattt atgcctgact
      421 atggaaataa tattcccatg agaggcttcc ttccggcatt tggacaagca gcgggtaaaa
      481 tattcctaca atcgtcgcag tggccatata atagcagtaa taaagaggtt acttacaact
      541 tattccatgc acatggcggc gcctttcttc aagtgtttac cgaagttcca caaaatttag
      601 ctgttactca caatcccatt ctttacagca gcgaatcttc atttagcgta attgctcctg
      661 caggagcttt tatttcatta acagtaaacg gagaaattat cggaacagcc atgagtaatg
      721 gcggttcgac aaatattgct attgagccac aattaccgcc taatactatg gtagttactg
      781 taacaaaaca aaactactat cgctacgaat ccaatgtaga tgttattcct ccaacaggcc
      841 catatgtagt aggtgattct tatgttatca acgatgcatc gggtaacaat aatggattga
      901 tggattacgg cgagagtgtc atgcttcata tgactatgaa aaacgttggt gttgctgttg
      961 caaataatgt ctctacgaca atgaccagta ataatgaata tgttacaatt accaacggta
     1021 atgcatattt tggcaatatt gcccctaatg gtacagtaac cgttaacaac gcattttcac
     1081 ttaccgtttc tccaaatatt ccggataacc atgctgtatc atttactctc aattctaccg
     1141 atggtaccga taactggatc agttatattt caattgttgg tcatgctcct aatattaaat
     1201 tcaaacagta tgttgtcaat gatgaaggtg gcaatggtaa tggtctgctt gatccgggag
     1261 aaacagctcc tatgataatc actgtagaaa atcaaggttc ttcagctgcc tataacgttg
     1321 ttggaatgct taccactctc gatccatacg ttactatact tacatcgcag cctcagaata
     1381 tgggcaacat aaatgccggt ggaacttcaa attgtacatt tacagtatct gccaatgcta
     1441 acatacctgc cggatataca gcagatctta cattaaatct caatgctgat ctcggtgtta
     1501 ctcaacaaac cactattcag ttgctctttc cagattattg ttatccgact gcaagctgtt
     1561 cctggggaga cggattcact ggttttgcac ttgagcaaat taataacatg accgggaatg
     1621 gctgtagtcc taatggatat ggcgactata ccaatatggt tgccgatttg gagggtggtc
     1681 aaacatattc agttcaatgg agaacaggat acagtaatca gcaggcatcg ctttggattg
     1741 acctgaatga taataaagaa tttgaaccat cagaaagact tattaccgac tttgttctct
     1801 cttcgtcaaa tcaaatttat acaactacat ttactgtacc cgcaggcagt tataccggaa
     1861 ataaacgaat tagaatacgt gccaattggc aaaactcttc gtccgatcca tgcgccaact
     1921 tcacttatgg tgaaaccgaa gattataccg ccaactttgg cagtcaaaca gttcttgcgc
     1981 caccgcaaaa tgtttcagca aacgtaacag gtaatagcgt agttgtaaca tggcaagcgc
     2041 cggcaggagc tgatgcaaca ttgttaggat ataatgttta tcgcgataac caaaaaattt
     2101 ctaatacgat aacatcacta acttatactg atacgaatgt accaacagga agtcattggt
     2161 attcggtatg tgctgtttat acagaaggtg aatcggagtt agcacaacct gtacaggtta
     2221 atgtaggttc attgcaagga aaattacaag gatttatcag aaatgctgtt actaatgcag
     2281 taattcaaaa tgcttgggtt tcagcccttg attctgactt tggagctgta acatataaca
     2341 ccatttttgg tagccattac acactttacc tttcagcagg aacgtattca gtatcgtgta
     2401 ctgctcccgg attccaaact cttacagtca ataatgtagt tatattggaa ggtcaaacta
     2461 aagcgcttaa cttttacttg catagctcct ctgaaagcaa tttgggtggg gaggttacag
     2521 gtgtcagtta ttttgaattc gaagattttt cagtttatcc aaatcccgcc tctagcgaga
     2581 taaacatcac aacaacatca gcaatgaagc atgttaagat tatgaataac atggggcaag
     2641 ttgtatttgt agatggtggc ggtgatctct taataagagt aaacactaca ggtttttcaa
     2701 gaggtatcta ttttattgag gtagaaacag aaaatcatag attctttgag aaattaatca
     2761 tcaactagtt ttcttaaaaa aaacttttta aaaaaaaggg agcgctttgt ttaaggctcc
     2821 ctttttttat ctccgatatt tttttaaaaa ccaagtgttt taagcattga tttgtacgac
     2881 tgttcttttg caaataattt tgggaaatag tcgccgtttt cgtcaagatc aatgatgata
     2941 tgctttggcg ccgggataag acaatgctga atcccgccat aacctcctaa tgattcctga
     3001 taagcaccgg tatggaagaa tccaatataa agcgtatcat ttttatcaaa tttgggtaaa
     3061 aagacagcat tggtgtgtgc ctcatcatta taataatcct ggctatcgca tgtcaaccct
     3121 ccaaggaaaa cacgttgata gtcggcctcc caacgattaa ccggaagtaa aataaaacgt
     3181 tggttaatag cccatgaatc aggtaaggta gtcataaatg agctgtcaat catgttccat
     3241 aactcacggt cattctgctg cttttgatca agtactgagt aaagtactgc gccactttcg
     3301 ccaactgtat atgaaccaaa ttcagtgaag atatctggct ctggtacacc atgttgttcg
     3361 caaaccgact tgatctgagc tattatttct tcggccatgt attcataatc atattcgaaa
     3421 ccaagtgagc gtttaatagg aaaaccaccg cctatattaa aagaatcgag ctcaggacaa
     3481 acttttttta attctacata cacattaata ctcttgttca actcattcca gaaataagca
     3541 gtgtccttga taccggtatt tataaagaaa tgtaacatct tcaatccaat tttaggattg
     3601 ttttttatct tttcatgata aaatggcaca atatcattgt accggatacc taacctcgaa
     3661 gtataaaagt taaacattgg ttcttcttct gaggcaatcc tgattcccac attaaagttt
     3721 ttgtccagat tatctacata gtagttaagt tcatccatat tatcgagaat ggggattgtg
     3781 ttttcaaacc cattgttaat aagacggata atattttcaa gatactgcac tcttttgaaa
     3841 ccattggata ctataattat atcctttgta attaatccaa tatcgtacaa ctcttcgatg
     3901 atgtggatgt cgaaagctga agaggtttca agattaattt tgtttttgag agcttcttcc
     3961 agaacaaaaa ggaaatgaga gcttttagtg caataacaat atttgtattc gccatgataa
     4021 tccaccttcg ccatagccac attgaacaat cttctcgctt tttgtatctg cgaactaatc
     4081 tttggtaaat aggtcagctt taccggcgtt ccatactgct tgatgatatc cattaacgga
     4141 atgttgtgaa aatataattg accatcaaca acattaaatt cctcttgtgg aaattcaaaa
     4201 gtttgctcga tcagatcaat gtattttact ttcatttttg taactaagtt agttagataa
     4261 tcaaaatggg tttaattcag caaacttaaa ttgctcaatt gtggcaaaag taaataattc
     4321 agattatagt aattggcaaa ataaataatt ttgttctatt aattttttga agtcttgatc
     4381 taactttagg gatgataaaa tgaattcatt gattgatagt gttttacgag agccttaggg
     4441 cttgtatgtt aaacatgttt ttcacccatg aggtacctca aagtcattcc gtgagttgtc
     4501 ttggaacaac cctgaaagta gaatttgagt tatctcgtga gtagtatcgg gtggaaatca
     4561 atgaaattta tacagctatg ggacataatt ctgtacccta ttataggaaa aagtttgcgc
     4621 ttttctaatg atgaacttcc aattggctaa caagttaata acaagatata tgaatgttat
     4681 gagacttcgg gttgggttaa tagttaagag atcatctctt tatgtccagt ccgaagagaa
     4741 tattttttga cagttaattg ggtttagatt ttttatattg cgacttgaaa atagattatc
     4801 tccattcgag agtggaaaga agatgatcaa tatccttgac aatgaaatta attaccggct
     4861 ccagggaatc attatttggt ctgacggaga agtaaagcgc tccgcgcaaa aaatgtgtag
     4921 aactatcact aacccaaaat tgaaagggtg aagcaacctc ttttccctca atcctataaa
     4981 ctaaaccaaa catcttgctt tcattgattt ttatagtgtt ttccttaata ccttgcgctt
     5041 tttcagcatg ttggaaaaca aaatttcttg catcttcagt aagattaaaa agtttattgt
     5101 cttttaacga tttgtagctt aagtggattc gtgctttata ctttgggtaa taaatattta
     5161 gccaaaacgg gtcatcagag gtataattat caaattgaat acgtgccgat tgtgaatatt
     5221 taaaatagta aggaaaagta gagtcaaagg gttggtattt gtgctcaggc agatctatac
     5281 gaaaatatcc ggctggacgt ggcaacattg gttggttttc acatgaggat attagcataa
     5341 taatcataat caaaccaatg gcaaataaat atccttttgt catagctcca actattgaac
     5401 acttccaggt tttggtaggg ttacctgtac ttccaatact ctacggctgt caaccttggt
     5461 gattttaaac tcgtattgct taaatttggt aatattattt aatttcggaa ttctcctttc
     5521 aatttcgatt ataaggccag ccagcgtatc gggatcacct tcgatgtctt cgaatatggt
     5581 atcctcaata cccgtgattt tataaaaatc attaagcaat gtttttcctt cgaaaaggta
     5641 agtgtagtca tctattttag aataacttaa ctcatcttca aaggtgtcga actcatcgct
     5701 tatgtcgcct actatttcct caattatatc ttcaagtgtt actattccgg aagtgcctcc
     5761 gtattcgtca accacgatag cgagatggat tttttttgac tggaattcct ttaacaaaaa
     5821 attaattttt ttattttccg gcacaaaaaa tgctggtcgc aaaagacttg cccagtcaaa
     5881 atcatctggt ttgtctatat gaggtaaaag gtcttttaca tacaggattc cggcaatctt
     5941 atcaaaattt tcattgtaag ctggaatacg tgaatatcct gaattaagta taatatcgag
     6001 cagtttatga tatgttgtta aaacatctac agcaactaca tctattcggg gtttcataat
     6061 ttctttagct tcgatatcac cgaatttgac aattcctttt aatattttat gctcttcggc
     6121 tggcgtgctt tctgtagcgg taagatcaat ggcctgtgat aaatcgccta atgagagttg
     6181 agccgatttg cggattaaac gtttatcaat aattcctgaa ctacttacca gcaatgcact
     6241 gaagggagaa agaaatttaa tcagatattt aaaaacagga gtaactaaca aggcaatttt
     6301 taaagctttt gaaactgaat aaatcttggg tataatttcg ccaaatatca atattattga
     6361 cgtaataaga actacctgaa tcaaaaattc aatgacagga ttatccttta attccacaat
     6421 agataaaagc aagaacgagg ataaaatgac aatagcaaca tttacgaaat tattagcaat
     6481 aagtattgtt gctaaaagtc gtttaggttg ttcaataagc ttgataagtt tttcactgtt
     6541 tttaatttta tctgtacgaa gttctctgag gtttgaaggg gtaagcgaaa agaaagccgt
     6601 ttccgaagct gaaatgaaag ctgaacagaa aactaaaaaa gcaattataa ttaaaccgat
     6661 gattacctga ggactaatag gatgcaggat aatactcgaa gcaataaata agggttttaa
     6721 gtttaaatat atatcaggat cgtccaagac attaaaagaa ttggttaaac ttgaaaaaaa
     6781 gatttccggg caaaaataat gacttcaatt aattaataga ttaaaaataa taaaagacga
     6841 aactctattt tgataaatag tttattatta ataagttaaa ctaaaaaggt gtaaaggtag
     6901 catttactct attaaaaagg caaatcatca gtaggagcat gttcaggagg tggtaaatca
     6961 ctatcatctt tatctggaac tattggtggc tggtattgtg agctccccga tgattccatt
     7021 tttcctagca tagtaaaagt gtcgccgaaa atttccgtga tatacttttt tatcccattt
     7081 tgttcatagc tacgtgtttt tatttttcct tcaagataaa tcaagctgcc tttacgcaga
     7141 aatttttccg caacttcagc taacccacgc catactacga tattatgcca ttcggtttgc
     7201 tctacacgat ttccctcttt gtctttgtat gtttcattag tagccaatga aaaggtagcc
     7261 ttttttacgc cattttcaaa agtcaggaca tttggatcat tgccaaggtt cccaaccaga
     7321 attactttgt ttatccctgc catagttgta agttttatgg ttttaattaa aggaacaaat
     7381 ataattcaaa tttgtgttct tttgacaaat ctatttaaaa atttgtttct ttttctgaaa
     7441 aaataaaatc aagattctat tttttttgag atatttgcca tgaatctttt aaatattata
     7501 aaaatgttct actttacaac tcagttttcg tccttggtta aacagggaaa attcgatttt
     7561 tttaaattaa tcgttttatt tgcgatgctt acttttttat ttagctgcaa tcagccaacg
     7621 cataacaagt tgcctcgtat tgcaattgcc ggtattgcga ttgagtcgag tacattttcg
     7681 cctgccgtta cttacgagga ggcttttttt gggaaaattg ccgatgaagt gttttcttat
     7741 tacccctttc ttgatgaaga ttctgctgtt cgcaaacgcg ctgtttggtt tcctactttt
     7801 cgtggtcatg caatgccagg tggtattgta gctcgagagg catatgaatc catgactgaa
     7861 aaaattttaa tcatgcttaa agaggcgttg cctttggatg gtcttctttt agatattcat
     7921 ggtgcaatga gtgtggtggg tttagatgat cctgagggag accttattgt gagaatacga
     7981 gaagtgatag gaaaagatgt cgttatttca acgaccatgg atttgcatgg aagtgtgagt
     8041 gaacgtttgg cagaaaatac tgatcttatc acctgttata ggcttgcccc acatgatgat
     8101 gtaattgaaa caaaaaaaag agcggttgta aatctgcttg atagacttga gagtggtaaa
     8161 ggaaaaccag cttacaaagt tcatattgta gttcctattt tgctacctgg cgaaaagaca
     8221 agtacacgta ttgaacccgc caaaagccta tattctcaga ttgatcctct tactaaaaaa
     8281 ccaggtatca ttgatgcagc aatttggatg tcgtatcctt gggctgatga accacgcaat
     8341 cacggtgtgg ttatggcaac cggcgatgat aaagaggcag tagcttcggt agcagagaag
     8401 ttggcctccg atttttggaa tgctcgtaac gattttgttt ttgtagctcc tactacaaca
     8461 ctggatgaat gcctggaaaa ggcaattaat agtgaagtta agccatttat tatcagcgat
     8521 atgggggata acccaacagc tggcggcgct ggtgatgtta cctggactat tactaaaata
     8581 ttagaaaacc ctacttttta taagcaggga ggtaaacgca taatttacgc ctcccttccg
     8641 gatgaaaagt tcacggaaga ggctttagct ttaggcgaag gggccagtat taaagctttt
     8701 gcaggcgcac gtgttgataa tcggtatgcg ccgccgatcc ttttggacgg aactattaaa
     8761 aaaattatgg agggtgacag ccgtgctaaa gtagaagttg ttgttgaggt aggaaatgtt
     8821 gatgtaattg taacgcaagg tcgcaaagca tatcattatg aagaagactt tacacgtctt
     8881 ggcttaaatc cacgtgaaac ggatatttta gtaattaaaa taggttattt ggcttcagag
     8941 ctttacgaaa tgcgtgctga ctggatcatg gcactaacac ccggtggtgt agatcaggat
     9001 ttggaacgat tagagtataa acgcgttcaa cgaccgattt tcccacttga taaaaatatg
     9061 gccgatccgg atttaagcgc cagatttatt atatcatctg atgatgtaaa atgatttttt
     9121 aataattgaa tcaaatttaa aggaagctaa atttttgatt taaagcattt agtcaatcca
     9181 attttatttt tatttgatgt ctaatttttg aatacttact tgtttgaccg agcaaatttt
     9241 aatgaaatat actgtcggct tgagatcatt aattagtctg aagttggttg ttttctattc
     9301 ccgcttcggt aatgcagata aatttttctt attaactttg ccggcgttta caaaggttgt
     9361 tgtttataaa aaaataagtg tatgaaactc ttttttgcct ttgaagtaat acatcataaa
     9421 aaattatttc tcctttattg gtattggtaa aaaagatttt taaatataga aaacaaaact
     9481 atagcgaatt cgttaaaaat cacattaatc aattttaaaa gtattttatt tatgaaattt
     9541 ctaaaatttt ttgcatttgc tttgctgatc tcatccttcg ccatattcac tggctgtagt
     9601 aaagatgatg aagatgtaga tcttggccca actatttcgt tgaaaacggg agaaggttac
     9661 acttcatcgg actttgaggt gtttaacgac agtacggttt tgtttgggat tgtagctaat
     9721 aagagtacaa ctcataacaa tttgctgtca aagttgaata tctattataa tgaattaact
     9781 ctgcttgatt ctacattgaa tacagcaaat ttctcgggag attttgaaat caaatttatt
     9841 ggtatcggaa caggtaagtt gcgttttaaa attactgctg aaggcgggct taccgatgag
     9901 acttcgctta atgttaccat acttgaatct cctgtcgttg gcgttgaagt ggataaaaat
     9961 agcggtgtag agctcggatc atttaacgat cctgttggga gcttttatag tacaaccgag
    10021 agaaaagctt ataaagttgc cgaagcaatg ttagagcaag gaaaaattga cttcctcttt
    10081 ttcaaagggg taacaaactt taatactatt gcggcacctg atgatgaaga tgcagctaca
    10141 atcagtgatt tccacttaac tgagtggacc actaaaaatc caacccgttt ttacctggcc
    10201 gatatcacgg cagaagattt tgatgctatc gaagataagt gtgttttccc tgaatttgat
    10261 gcagaaaagg ctgaaacaaa agccaatgag ctgaaaaata atgacgttat tttctttaaa
    10321 acagtaggcg ataaattggg atacataaaa attgttgatc tctatcatag aggcgataga
    10381 ataaaatttg atgtaattgt aatgaaataa ataagcattg attttaaaat aaaattgccg
    10441 gtttgattat ttattttcaa accggttttt ttttatctga tagattttcg taaacaaaga
    10501 acaaaattta ggtgtcataa cgattctatt gaaacgggaa tgctaatttt gctgctttac
    10561 taatttcttt atgattgacg cattatttga aagccttctg cagcagcaac agttttcatt
    10621 atttttttat acgtttgttt tacttctatt cattcagctt atcttctttt tggtatgttt
    10681 taaccggctg aattcaagga aactacaaaa agaaattgat gttaagcaat cggtatccgt
    10741 tgtattggtt acaagtaatc agtacaatga tttacgtaaa ctactgcccg aaatcctcaa
    10801 tcagagctat tctaattatg aagtagtagt agttgacgac tcttcagaag atggtagcga
    10861 tgtgcttctt gaaaggtttt cagagcaata ttctcatttg aaggtagtca atatgcatca
    10921 acatttgaat tggtttaaag gcaatgaatt tccaatatct ataggtatca agtcggcggt
    10981 aaatgactgg attctgctta ccgaaacaac atgtatccct cgtagcagcc attggatcag
    11041 cgagatgatg aaaaaagcta attttccaac ccaaattgtt cttgcacgtt catcttttgc
    11101 tactagttca aaaattaaca tttggtatcg ttttgcgagg ttttataaca gtttgattaa
    11161 tctatctttt gctatttcca aaatctcttt taaaggatca actcaaaatc ttcttttcag
    11221 tcgtaagctt ttctttgacc aaaaaggttt cgtttctcat tacaccatta aagggggtgc
    11281 cggtgaactt tttgtaagtg aggctgctac actatcaaat gtaagtgttc aaatctcccc
    11341 aaaaagcgtt gtagattatg taaaatcaac ctcgttcaga aaatggatta tcaaagaaca
    11401 cacaaacctt gatatagagc gccgaattaa cttaaagcaa cgaattcctt ttttactcta
    11461 ttttaattcg ttatttatct tttatttagt atttggtatt ctgatgatca acaacttgca
    11521 ttggcaagtt attcttagtc ttttttcgat acgattggtc agtcagtttt ttatttttca
    11581 ccgtgcacaa aaaagacttt cagaacaaag attgttgttg ttaatgccgg ttttcgaaat
    11641 agcgctttta gtcatacatt tcttaatacg tttcaggctt atttttataa aaaaaggagt
    11701 atgggtttag gtaaaacaaa gtatattttt gatgaagtca ttagtcgtaa gggtactgac
    11761 tgtgtgaaat atgatcttcg gaagctctat tttgggaccg aagatgttat tccaatgtgg
    11821 gtggcggata tggattttcg tacacctgat tttattattg aagccattcg taggcggctt
    11881 gaccatgaaa ttcttggtta ttcgatccgt tcagaggagt atagttcggc tatttccaac
    11941 tggatgttag ttcgtcataa ttggcaaatt aatccggcat ggatcagctt tagccctggt
    12001 gtggtgcctg ctttgaatat gttggtttta gcctttacag agccgggaga taaaatcatc
    12061 gttcaaccac ctgtttattt tccgtttttt gctgctatag aaaacaatgg gcgcaaaaag
    12121 gttgagaatc cattaagatt gattgaggga agattaagta tggattttga tgatctgcga
    12181 aaaaaagctc agggagctaa aatgatttta atcagtcatc cgcataatcc gggaggtagt
    12241 gtgtggacta aagaggagtt gaccagctta gccgatattt gtttagagaa taatgtgctg
    12301 atgatttcgg atgaaataca ctcagatctt attttttcgc cacacaaaca tatacctttg
    12361 gcatcgctat ccaaaaaagt ttccaatcaa accataactt gcaatgcccc ttcaaaaaca
    12421 tttaatctgg ctggcctttc cacctcttat cttattattc ctgataaggc aaaactgaat
    12481 gtatataatt cgatactgaa tgataaacta catttgaata tgggaaacat agccggttca
    12541 gttgcgctac aggctgcata taactttggc gctgattggc ttaaacaatt actcgaatat
    12601 gtcgctaaaa atgtggaatt ggtttgtgtt ttttgcaatg agtatttacc acaaattact
    12661 cctattatcc ctgaatctac atatttgata tggctcgatt gccgtaaaac aggaatgaaa
    12721 ggcgatgatc ttaaagattt ctttatcaaa aaggcccggg ttggttttaa cgatggcagg
    12781 ctttttggaa ctgggggcga aggttttatg agaattaatg ttgcttgtcc cagatcggta
    12841 gtattagaag ctctgcaaag aataaaaaat gcctttcaat atctttaaag ccttaattga
    12901 gaaaactgaa atccaggatg tcaaacttaa cctttaaaca tttttattaa ctttgcctta
    12961 caattttgtg aatcaaattg catgaaacat caattacaat ttgtgctcct ttattgtatt
    13021 ttttgtttag ccttgaataa taaggctaat gcaatagctc ctattatcca ctcagtaata
    13081 ccgaataaca ccacaattgc cagatacgaa aaattcgaac ttattgtaga tgtaacagct
    13141 acgtatgctt ctccttacga ttttgaccag gtaaatttac aggccacttt tacttctcct
    13201 tcgggaaatg tatttgtagt agatggtttt tattatcagg attttacaat gacgcagcct
    13261 aatgtattaa ctccaaatgg cgcacctgac tggcgaattc gtttttctcc aaatcaaaag
    13321 ggggtttgga gttatacagt taaaattact gacacacaag gtacagcttc atttccatca
    13381 caacaattta cgtgtattac ctctttacac aagggttttg taaaaagaag cggaaataat
    13441 ttagtttacg acaatggcga tagatttctg gcgataggaa ccaatctggc atggacggaa
    13501 tgggcgtcag gttttacaat ttacgatgaa tggattacct ccctaaaaag ttatggcgct
    13561 aactatgtaa aaattaccat ggctccatgg atatttgggc tggaatgggg gtcgggagca
    13621 atgggaaatt atgcgggtcg ccaaaataga gcatgggcgc ttgattgggt attcgataaa
    13681 ctagaacaaa aaaatatata ttgccagttg cactttcttg ttcatgatga actaagaacg
    13741 ggagttaatc cggggtggga tgataatcca tataaaaata ccaacggagg cccttgtgca
    13801 aacccacaag atttttttac aaattccact gctaaaaaat atttcaaaca aaagcttaga
    13861 tattccaacg cacggtgggg ttactcttca tacttacatt cgtgggaaat aatgtcggaa
    13921 gttgataatg taagttttta caacagtaat tatagtcaga tatttaattg gttgttggag
    13981 ataactaatt atgtaaaaag taaagatgtt tattcacgtg ctgtatcatc aggctatgca
    14041 tggccacaaa atgattcggc gtattggaat aatagcgcag taggttactc ccagttacat
    14101 atttatgatt ttatttctga tctggaaatg aaattatata attattcacg tcattatatc
    14161 gaaaagtatc aaaaaccaaa cattatcagt gagtttgctt taggacacga gccaaacaac
    14221 atatttcaaa atgatccgga gggcgtagcc tttcacaatg ttttatggtc gtcagtattt
    14281 tctggtacaa tgggtaccgc tatgtct
//
LOCUS       DAOOAI010000058        14252 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_5509_length_14252_cov_5.103332, whole genome shotgun sequence.
ACCESSION   DAOOAI010000058 DAOOAI010000000
VERSION     DAOOAI010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 14252)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 14252)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14252
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5509_length_14252_cov_5.103332"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(355..2052)
                     /gene="ftcD"
                     /locus_tag="PK345_05525"
     CDS             complement(355..2052)
                     /gene="ftcD"
                     /locus_tag="PK345_05525"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HPX34510.1"
                     /translation="MKKLIECVPNFSEGKDMSIIKQITDQIETVEGVKLLDVDPGAAT
                     NRTVVTFVGTPDEVIEAAFLAVKKASQLIDMTKHKGEHPRMGATDVCPLVPISNISME
                     ETVEYAHKLAKRIGDELEIPVYCYEKAAFTKERRNLAYCRSGEYEGLPEKLSKPEWKP
                     DFGPAKFNARAGATAVGARDFLVAYNINLNTTSTRRANSIAYDIRERGRVKREGNPIT
                     GKIVRDEQGNQVYIPGSLKEVKGIGWFIEEYGIAQISLNLTNISVTPVHVAFEEACKK
                     AQERGIRVTGSELVGLIPLKAITDAGKYFLRKQQRSVGVSEDELIKIAVKSLGLDDLK
                     PFNPKEKIIEYMISEDHSKKLADMDLRSFANETASESPAPGGGSISAYVAALGVSLGT
                     MVANLSSHKRGWDDRWEEFSDWAEKGQALKDELLLLVDEDTHAFNLIMNAFGMPKSSD
                     NEKLLRTKAIQEASKYAMEIPFRVMQKSLESMEIMKAMALTGNPNSASDAGVGALCAR
                     TAVKGAFMNVKINAAGIDDHAFVTNLLEKGAAIEKMAEEKEREIVTIVEQKIKELAK"
     gene            complement(2129..3394)
                     /gene="hutI"
                     /locus_tag="PK345_05530"
     CDS             complement(2129..3394)
                     /gene="hutI"
                     /locus_tag="PK345_05530"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HPX34511.1"
                     /translation="MKDIIILNIKELIQVEDSPRLMVKGAEMAELTTIKNAFLHISNG
                     LIASFGSMEIFGGKFNSRQLDNSKIIDATGKMVFPSFCDSHTHLVYPASREIEYVDKI
                     KGLSYEEIAKRGGGILNSARRLQDISEEELTCSAFERLKEIISFGTGAVEIKSGYGLT
                     TESELKMLRVIRKLKEISPLTIKATFLGAHAVPLEYRFRQTEYVDLIINEMIPMVASE
                     ELADYIDVFCDRGFFTVEDTDRILNAGMKYGLRPKIHANELDYSGGIQVGVKYNALSV
                     DHLEYTGDAEIEALKDSETMPTILPGAAFFLNMPYAPARKMIGAGLPVAMASDFNPGS
                     SPSGNMQLVLSMASILYRMTPEEGINATTINSAYAMDLSREMGSIAVGKIANVFITKP
                     IPSYQYMPYYYGSNKVDTAILNGVVECVN"
     gene            3722..5842
                     /locus_tag="PK345_05535"
     CDS             3722..5842
                     /locus_tag="PK345_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C10 family peptidase"
                     /protein_id="HPX34512.1"
                     /translation="MKLKNLFLMMMFFFGISILFAENVTVQDAERVATRFYNEKYVVN
                     FPGEEANFYVVNNHIIDNNGLPAVYIFNFNNGGYAVVPADDRLYPVLAFSFNGAFDPE
                     NTPSNWWYVLNDYGAQVTHVRKNNVEATDRAAAAWINLRNEATGNLSFLTNIRDIPVL
                     MTNIWNQDYPYNYLCPEDPQGPGGHVYSGCVANSMAMIMYHWRFPYTGTGSKTYYPAG
                     YGPQTANFGESYYDYEGMVDVVGSTPNYPVALLHYHCGVAVNMMYSPNGSGAYSQDVV
                     PAIKNYFKCSNTAQFIQRGGWPAWKAYLDQQLELEQPIYYSGQDPEGGHAFVCDGLQE
                     QPDNTYYHFNFGWGGYMNGWYTAENAGGFNSNNAIVRNFIPDESMYPYSHPTELVTIS
                     NLVGTLDDASGPKHNYQPNVNSRWLLSPQTEYDSVSYIKINFTRFATEENADFLRIYD
                     GEDETAPLLGEFSGTEIPGEVTSTNNKVLLVFTTDDTNEDNGWLISYKAYQPTWCSGL
                     TNFTEPTGTFDDGSGSFYYNNGTTCMWTIQPKWANKTTISFNYFDTEDGKDLLKVYDL
                     QSQQLLASLSGNTIPDPITSNSGKFYLVWTTNNVNRGLGWEINYEADNVGINENDDVF
                     NNLSIYPNPADDLLNISFISKESDYVNISLINMTGIEVYKNVAHAKSTVFTNSIDLSD
                     LAKGVYVLRLQSERKDLIRKIVVE"
     gene            complement(6066..8222)
                     /locus_tag="PK345_05540"
     CDS             complement(6066..8222)
                     /locus_tag="PK345_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011107669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HPX34513.1"
                     /translation="MYIEDQIKIIATGLGIKPKQVTSVLLLLEQGATIPFVARYRKEL
                     TEELNEEEITAIRDGYEKLTEITKRRESIINTLKETGQLTDDLELQLNNAVTLSELED
                     IYLPYRPKRKTRASVARDRGLEPLAKLLMRQQYDDVEKTARKYVNFEKEITTVEHALE
                     GARDIIAEWVSENKVARERLRTLYRKRATIKSEVVKAKIEMAQNFSNYFDFEEPLDRS
                     PSHRILAMLRGENEGFLRLQIAPPPNLALQILEKIFVKNESSAAQHVLLAVQDSYKRL
                     LQPALENEFRAEAKERADKEAIRIFVDNVRQLLMAPPLGQKRVLAIDPGFKTGCKIVC
                     LDEQGKLLHNETIYPHPPQNEVRQSIAKIEQLVDAYKIDAIAIGNGTGGRETERLVRH
                     LRFNREVIAVMVNESGASVYSASSVAREEFPDYDVTVRGAVSIGRRLIDPLAELVKID
                     PKSIGVGQYQHDVNQTQLARSLDDTVMSCVNAVGVEVNTASKQLLAYVSGIGPSLAQN
                     IVSFRNQNGAFDSRQALNKVPRFGPKSFEQAAGFLRVNTSANPLDKTAVHPESYHIVE
                     KMAKSLGVTVEELMKNADLQSKINIEEFVTDQAGIPTLKDIVEELARPGRDPREKFGV
                     FQFSMGINTIKDLKEGMILPGIVTNITAFGAFVDVGVHQDGLVHKSKITDRYVNDPSE
                     FFKVNQRVKVKVENVDLERKRIQLTMIGVEQPKYVF"
     gene            complement(8226..11465)
                     /locus_tag="PK345_05545"
     CDS             complement(8226..11465)
                     /locus_tag="PK345_05545"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HPX34514.1"
                     /translation="MPTTTSKNFVVFKSSAGSGKTYTLVKEYLKLVISNPERYSKILA
                     ITFTNKAANEMKERIVRSLKELSSFYTEQPDDKIISLMTTLEEELGFDARKISSRATV
                     ALSLILHNYSRFAVSTIDSFVHKLVRVFSRDLGLPAAFDVELETEKLIAKTVDLLISK
                     AGVDKDLTKILISFISSKMEDEKNWNIKKELKDFTQNIIEERTYEALKNISKLSPSDF
                     LEISHKLLKIKNDFENRIKKTAQRASNLIDKKGIDLKAFSHGLKGIGNYFKKIAEGNF
                     STIKPNSYIQKTINEDKWTSASCSAADKNSIATIKDELITDYNEIQNILEKDYDEYLL
                     SQLVGRNIYLMAILSEIEKVMEEFRQNENIVHISEFNKRIAAIVQREAIPYIYERIGE
                     RYRHFLIDEFQDTSLLQWQNMIPLLENSLSANNFNMIVGDGKQAIYRWRNGEVEQFAA
                     LPQLYKRGNDETSITREKLFVSQSRLKNLDKNFRSAIEIIRFNNAFFEFAKSKIAPDL
                     QRIYDGLEQEGNLNKSGGYLQFEFLKDNVDDNNDEVGELVREKRVFEILKELEDTNLF
                     ARQEIAILVRKNKQAIKVANYLLQQGITVVSSEALLLGSSPKVRVLMALLEFFIEQEN
                     RQVITEILFYFFKRGKFGEASFHQALAQCKEISPDEPASISMNETLKGFGLDLTQPHM
                     HTLSLTDIFEFFIMALQPDIGDNDPFVTFFLDMVYEQSQKQPMSYQDFLDFWNDTGCK
                     KSIIIPEDTPSVRVMTIHKAKGLQFKVVICPYMFENVRNSQNSAWIEPGFKSIPELKF
                     SRIPLISALKDTSYQELYQRESDKSFLDMLNLLYVAFTRPEKRLYVISKDKRKDNQKW
                     SFATPQPDMSDILFEFLNENNIPKDENERFVIGEKISLSKPGNKFDISTEKAYNNNAR
                     NSDAKITSANWRYKIKIRQRANQFWTESFVDKQRDFGLLLHRIMARISTLEDLDPVLK
                     DLQNNGTVQPEQVPDLKSEIQKVISRPEINKYFQKGVKAYNEKDILLNDSTVLRPDRV
                     VIDGNKVIVIDYKTGKKDAKHKQQMTEYIEALRQMGYLHSEYHLVYLNP"
     gene            complement(11469..12578)
                     /locus_tag="PK345_05550"
     CDS             complement(11469..12578)
                     /locus_tag="PK345_05550"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34515.1"
                     /translation="MRYIFNFRVPTTFSYLVVIILFSCQTSVFSQITITQNDMPSAGD
                     TVRKSFALDFTGIDFSVTGENYLWDFSQLKPASQTVDTFVTVSSVPFLYQLVFIPNIV
                     ANVAQKYTGLENFPQVPITDPYRFYKKSSNAFNDVGYAATISGIPVPLRLNPADVIYK
                     LPLAYGNIDSSDASGQLGVPDIGYVSIQRKRLNEVDGWGTLITPYGEYDVLRLKSSVM
                     ETDSVYVDTLNFGQQIVRKYTEYKWLTNGKKQPLLEVTEENAITQVTYIDSIFDPGVG
                     IEKADIRKPKIYIAPNPIIDKAIIVVELSEPEYITISLINMAGIKIKRVFSGNLPAGK
                     HFQTLNLSDSKPASGVYLIQVEWNGKSTAKRLIIQ"
     gene            12940..>14252
                     /locus_tag="PK345_05555"
     CDS             12940..>14252
                     /locus_tag="PK345_05555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34516.1"
                     /translation="MSNPTIQTLVSRYAAVWLTHHIGTEVHIGSINLGIRGTFLLKNL
                     MIKDKYDDEMIRVGELEILITGISNKNKEVSLGLLELKDFGFQLLKHHDAEMTNFSYF
                     LQNFVSKTAEDTTEESKLPWSIKCRSIIISNGYFKYSDDDFLTENYKGIDFNDIELNA
                     RQIKISKFHFDHDTLKANIKNIELIEKSGFKINSFEGLLTLNPTLLEVDQLVAETNNS
                     KLNADLRFVLNGYDAFSNFIQDVQIDGFFRPTIINLSDIGYFEPSLFPMVDEVELKGQ
                     IAGTIADLKAKKLEFNYGKNTSFIGSLNIKGLPEIDTTFFNANIQHFTTNANDLRSFN
                     FPGDKQKIEIPEIVKKLGNINISGTFAGTYNQFQSGLKVKSQIGFISGQLQVNQSAGK
                     NLVRYSGMLLAKDFDLGKLVDSEQLLGKTSFSLNILTALMGSPTY"
BASE COUNT         4141 a         2865 c         2575 g         4671 t
ORIGIN      
        1 agtttggcgt ttgtgagata ccgccattgt tatgaatttt ccggcttact ccacatccgt
       61 tttggtttta aacttacact tatggcttat actccttcgt ggaatgtgag ttgttaagga
      121 tgggctatga gaagatagaa tttgaacttt ttaccggcga tgggatttta acactaacaa
      181 aacagttgaa ttactgtaaa tatctttttt tatagtcaga ttttcagaga ggttttatta
      241 aattataagg tgaatgtaac cccactaaaa aattgcagtc agcttcagga agataaaatg
      301 atcaaaatta tgcttcaacc taaataatgc tacttatttt tcttagccat cttactattt
      361 ggccagctcc ttgatttttt gttcaacaat tgtaactatt tctctctctt tctcttcagc
      421 cattttttcg attgcagctc ccttttctaa taggttggtt acaaaggcat gatcatcaat
      481 tcctgcagca ttgattttta cgttcataaa agctcccttt acggcagtcc ttgcacacag
      541 tgctcctacg ccagcatcgg atgctgaatt aggatttccc gttagcgcca ttgccttcat
      601 aatttccatt gactctaacg atttttgcat cacccgaaat gggatttcca ttgcatattt
      661 tgaagcttcc tgaatagcct ttgtgcgtaa cagtttttca ttatcactac tttttggcat
      721 cccaaaagca ttcatgatca ggttaaacgc atgtgtgtcc tcatctacta ataacagaag
      781 ttcatcttta agcgcttgtc ctttttctgc ccagtcggaa aattcctccc agcggtcatc
      841 ccaaccgcgt ttatgcgacg acaggttagc taccattgtt cccaacgaaa cgccaagcgc
      901 tgccacataa gcagatatgg aacctccgcc cggagccggc gactcggagg cagtctcatt
      961 ggcaaaacta cgtaaatcca tatccgccaa ttttttagaa tgatcctccg agatcatgta
     1021 ttcgatgatt ttttctttcg gattaaaggg tttaagatca tccaatccca atgattttac
     1081 tgctattttt ataagttcat cttcagaaac gccgactgag cgctgttgtt tacgtaggaa
     1141 gtattttcct gcatcagtta ttgctttaag aggaataagc cctaccaatt cggagccagt
     1201 aacgcgtatt ccgcgttctt gagccttttt acaagcttct tcaaaagcca cgtgcactgg
     1261 agttacactg atattagtta aattcaaaga tatctgagca ataccatatt cctcgataaa
     1321 ccatcctatt ccttttacct ccttgagcga tcccggtata tatacctgat ttccttgctc
     1381 gtctctcact atctttcctg taatgggatt accttcccgt ttcacacggc ctcgttctct
     1441 tatatcataa gctattgaat tagcccgacg agttgatgtt gtattcagat taatgttgta
     1501 agctacaagg aaatcgcgtg cgccgactgc agtagcgcct gcccgtgcgt taaacttagc
     1561 agggccgaaa tcaggtttcc attcaggttt cgacaatttt tcaggtaacc cctcgtactc
     1621 gcctgagcgg caataagcaa gattccggcg ttctttggta aaggctgctt tttcataaca
     1681 gtaaacaggt atttccaatt catctccaat tcgtttggcg agcttatgag catattccac
     1741 agtctcttcc atagaaatat ttgaaatagg taccaaaggg cacacatcag tagcgcccat
     1801 acggggatgc tctcctttat gttttgtcat atcaatgagc tgcgaagcct tttttacagc
     1861 caaaaaggcg gcctcaatca cctcatccgg cgtgccgaca aaggttacaa cagtgcggtt
     1921 ggttgcagcc cccggatcta catcaagcag ctttactcct tcaacagttt caatttgatc
     1981 ggtgatttgt ttaataatag acatgtcttt accttcgctg aaattcggaa cacattcaat
     2041 caactttttc attttcgatg atttatttta taaattattt cttaaaaaat gatcttttta
     2101 acttatgtca aaccaatcta atatcttatc aatttacaca ctccacaact ccgtttagga
     2161 ttgcggtatc cactttattg ctcccataat aatatggcat gtattgatat gacggtatgg
     2221 gtttggtgat gaagacgttg gcaatttttc ctacagctat actccccatt tctctgctaa
     2281 ggtccattgc ataagcgcta ttaatggtag tggcgtttat tccttcttca ggcgtcattc
     2341 tatataaaat gcttgccatt gataaaacaa gctgcatatt tcctgaaggt gaagatcctg
     2401 ggttgaagtc gcttgccatg gctacaggaa gtccggctcc tatcattttt cgagcagggg
     2461 cataaggcat atttaaaaag aatgcagcgc caggcaaaat tgtcggcatg gtttccgaat
     2521 cttttaatgc ttctatctca gcatcacccg tatattcaag gtgatcaaca gacaatgcat
     2581 tgtattttac tccaacctgt attccaccgg aatagtcaag ctcgttggca tgaattttcg
     2641 gtcgcaaacc atatttcatc ccggcattaa gtatccggtc ggtatcttca acagtgaaaa
     2701 atcccctgtc gcaaaaaaca tcaatatagt cagccaactc ttcggaggct accataggta
     2761 tcatttcgtt gataattagg tcaacatatt ccgtttgtct gaacctatac tcaagcggta
     2821 cggcatgtgc tcctaagaag gtggctttta ttgttaatgg cgatatctct ttaagttttc
     2881 tgattaccct caacatcttc agctccgact ctgtagtaag tccataacca cttttgatct
     2941 caacagcgcc ggtgccaaag cttattattt ctttgagtct ctcaaaagca gaacaagtca
     3001 actcttcttc tgagatatcc tgcaaccggc gcgctgaatt taatatcccg ccgcctcttt
     3061 tggcaatttc ttcataagag agccctttga ttttatctac atattcaatt tcgcgactgg
     3121 ccggatacac caagtgagtg tgcgaatcgc aaaaggaggg gaaaaccatt tttcctgtag
     3181 catcaataat tttggaatta tcaagttgtc ggctgttaaa tttaccccca aaaatctcca
     3241 tacttccaaa cgatgcaatt aatccgttag aaatatgcaa aaaagcattt ttaattgtag
     3301 tcaactcagc catttcagcg cctttaacca tcaaacgggg cgaatcttct acctgaatta
     3361 attccttaat atttagaata atgatatcct tcatgttgtt aaatttttga caaaaatagt
     3421 ttaaagcttt tgaatcacag atcaagcgct tgacaatgcc taaataattt agtgtttgta
     3481 atcagcatta aaaagccata ctgaccattc actattttgg tattggcctt taaaaatcaa
     3541 tagttcggcc taaaggaggt tattgttaat tagggtttaa atagccagtt atctcatttg
     3601 gaaattttta aaactcaaat atcaaaaata aattactttt gtggtaatta taatattaaa
     3661 aaagcaattc aattaactga atattaacac tttaaataaa tttatattaa aattctcgat
     3721 catgaaatta aaaaatctat tcttaatgat gatgtttttt ttcggcattt caattctttt
     3781 tgccgaaaat gtaacggttc aggatgctga aagagtagcc acccgatttt ataacgaaaa
     3841 gtatgttgta aatttcccag gcgaagaggc taatttttat gtagtcaaca accacattat
     3901 tgacaacaat ggtttacccg ctgtttatat tttcaacttc aataacggtg gttatgcggt
     3961 agttccggca gatgaccgtc tttatccggt acttgccttt tctttcaatg gcgcttttga
     4021 tcctgaaaat actccctcca actggtggta tgtactcaat gattatggcg cacaggttac
     4081 tcatgttcgt aaaaataatg tagaagccac tgatcgtgct gctgccgcct ggattaatct
     4141 gagaaatgag gctacaggaa atctttcctt tttgacaaac atcagggata tacccgtttt
     4201 aatgaccaat atttggaatc aggactatcc ttataattac ctatgtcctg aagatcctca
     4261 aggaccgggc ggacatgtat attccggctg tgtagccaat tctatggcaa tgataatgta
     4321 tcattggcga tttccttata ccggtactgg cagtaaaaca tattatcctg caggatatgg
     4381 tcctcaaaca gccaattttg gcgaaagcta ttacgattat gaaggcatgg tggatgtcgt
     4441 aggcagtaca cccaactatc cagttgctct gttacactac cattgtggtg ttgctgttaa
     4501 tatgatgtat agtcctaatg gatctggagc ttacagtcag gatgttgtac ctgcaattaa
     4561 aaactatttt aaatgttcta atacggctca atttattcag agaggaggtt ggccggcatg
     4621 gaaagcatat ctggatcagc aattggagct tgagcaaccg atttattatt cgggtcaaga
     4681 ccctgaggga ggccatgcat ttgtatgtga cggcttgcag gaacaaccag ataatactta
     4741 ttatcatttt aatttcggct ggggtggata tatgaatggc tggtacaccg cagaaaatgc
     4801 cggaggtttt aattccaaca atgcaatagt cagaaacttt attcctgacg aatcaatgta
     4861 tccttattct catccgacag aattagtaac cattagtaat cttgtcggaa cacttgatga
     4921 tgccagcggg ccaaaacata attaccaacc aaatgtaaac agccggtggc tcctctctcc
     4981 gcaaactgaa tatgactctg tctcttacat taaaattaat tttaccagat ttgcaaccga
     5041 agagaatgcc gacttcttaa gaatttatga tggtgaggac gaaaccgccc cactacttgg
     5101 agaattttca ggcacagaga ttcccggtga agtaacttcg acaaataaca aagtgctatt
     5161 ggtttttact accgacgata ctaatgagga taatggctgg ttaatctctt acaaagcata
     5221 tcaacctaca tggtgctcgg gactaacaaa ttttactgaa cctacaggca cttttgacga
     5281 tggttccggc tcattttatt ataataatgg tacaacctgc atgtggacaa ttcaacctaa
     5341 atgggctaat aaaactacaa tttcattcaa ctactttgat acagaagatg gaaaagactt
     5401 attgaaagta tatgaccttc agtcgcaaca attacttgcg agtctttccg gaaatacaat
     5461 acccgatccc ataacaagta acagtggtaa gttttatctt gtctggacta ccaacaatgt
     5521 aaatcgtggt ttaggatggg aaataaacta cgaagccgat aatgttggta ttaatgaaaa
     5581 tgatgatgta ttcaacaact tatcaattta tcctaacccg gcagatgact tgctgaatat
     5641 ctcttttatc tcaaaagaga gtgattatgt aaacatctcg ttgatcaaca tgacgggaat
     5701 tgaggtatat aaaaacgtgg cacatgcaaa atcaactgta tttaccaatt ctattgactt
     5761 gagcgatttg gcaaagggtg tgtatgtctt acgtttgcaa tctgagagaa aagatctgat
     5821 cagaaaaatt gtcgttgaat aatacaattt gtattcttct gtaaaaagca gaaagaggct
     5881 gtcgtgttag gtcagcctct tttttttacc atgatttatc acctctctgt aaaaaaacct
     5941 cgataactga acgccgattt agtgtttgta tttagcataa taataattgc actgattaaa
     6001 acattaagcc gaggttggga ttataaaatc tatagttcga tcaaaagatt tttctattga
     6061 atgattcaga acacatattt tggttgttca acgccaatca ttgtcaattg tattcgtttc
     6121 cgttctaaat caacattttc aacctttact tttactcgtt gattgacttt aaaaaactct
     6181 gaagggtcgt tcacatatct atcagtgatt ttacttttat gcaccaggcc atcctgatga
     6241 actcccacat ctacaaaagc gccaaaagca gtgatattag ttacaatacc tggtaatatc
     6301 attccctctt tcaggtcttt aatagtgttg atccccatac taaactgaaa tacgccgaac
     6361 ttctcacgtg ggtcgcggcc gggccttgcc aactcttcaa caatatcctt taaggtaggt
     6421 atgccagctt gatcagtaac aaattcttcg atatttatct tactttgcag atcagcattt
     6481 ttcattagct cctcaaccgt tactcctaat gattttgcca tcttttcaac gatatgataa
     6541 ctttcgggat gcaccgctgt tttatccaat ggattagcgc ttgtattgac tcttaggaac
     6601 cctgctgctt gttcaaagga tttggggccg aagcgtggga ctttgttgag cgcctgacgt
     6661 gaatcaaagg cgccattttg gtttcggaat gatacaatat tttgtgccaa tgatggacca
     6721 atccccgaca cgtaggctaa taactgttta cttgccgtat ttacctcaac tcctactgca
     6781 ttaacacagc tcatcacagt atcatcaaga ctacgggcaa gctgtgtctg attgacatcg
     6841 tgttgatatt ggccaacgcc aattgatttc ggatcaattt ttactaattc ggctaacggg
     6901 tctattaatc gacggccaat agatacagcg cctcgcacag taacatcgta atcaggaaat
     6961 tcttcacgtg ccacagagga agcagaataa accgaagcgc cgctttcgtt taccataacg
     7021 gcaattacct ctcggttaaa ccgcaaatgc ctgactaacc gctccgtctc gcgtccgcca
     7081 gttccatttc caatcgcaat agcatctatt ttatatgcat caactaactg ttcaattttt
     7141 gcgattgact ggcgcacttc attttgtggc ggatgtggat aaatggtttc attgtgtaaa
     7201 agcttgcctt gttcgtcgag acaaacaatc ttacatccag ttttaaaccc cggatcaata
     7261 gccaaaactc gtttttgacc taatggtggc gccatcagca attgacgtac attatcaaca
     7321 aaaattctga tcgcctcttt atcagcgcgc tctttagctt cagctctaaa ttcgttttct
     7381 aaagcaggct gtaaaagacg cttgtaagaa tcctgaacag ccaataatac atgttgtgca
     7441 gcgctgcttt cattttttac gaaaatcttc tctaaaatct gtaaagcgag atttggtggt
     7501 ggcgcaatct gtaacctcaa aaagccctca ttttcgccac gaagcattgc aaggatacgg
     7561 tgagacgggc tacgatcaag tggctcctca aaatcgaaat agttagaaaa attctgagcc
     7621 atttcaatct ttgctttaac gacttcagat tttattgtag cccttttccg gtaaagagtt
     7681 cgcaaacgtt ctcgtgcaac tttgttttcg cttacccatt ctgcaataat atcacgagcg
     7741 ccttcaaggg catgttcaac tgtggtgatc tctttttcaa aatttacata tttgcgtgca
     7801 gttttttcaa catcatcata ttgctgtcgc attagcagtt ttgctaaagg ttcaagtcct
     7861 ctgtcgcgag ctacacttgc tcgtgttttt cgtttagggc gatagggcag gtagatatct
     7921 tcaagctcgg aaagcgttac agcattgtta agttgtaatt caagatcatc agttaactgg
     7981 ccggtctcct tgagtgtatt gattatgctt tcgcgtcttt ttgttatttc agttaatttt
     8041 tcatagccat cacgtattgc tgtaatctcc tcttcgttca gttcttcggt taattctttc
     8101 ctgtaacgcg ccacaaaagg aattgtagcg ccctgttcta acagcaaaag cacgctcgtt
     8161 acctgtttag gtttgatacc caaaccggta gcaattattt ttatttgatc ttcgatatac
     8221 attttttatg gatttagata taccaaatga tattcggaat gaagataccc catttgccga
     8281 agcgcttcaa tatattcggt catctgctgc ttatgtttcg catctttttt tccggttttg
     8341 taatcaatta ctattacttt atttccgtca ataacaaccc tgtcgggacg tagaacggtt
     8401 gaatcattta acagaatatc tttctcatta tacgctttta ctcctttttg gaaatattta
     8461 tttatttcag gtcgggaaat caccttttga atttcggatt tcaaatcagg tacctgttcg
     8521 ggttgcacgg ttccattatt ttgcagatcc tttaaaacgg ggtcaaggtc ttctaaagtg
     8581 gaaatacgcg ccatgatcct atgcaataat aacccaaaat cgcgctgctt atccacaaaa
     8641 gattcagtcc aaaactgatt tgccctttgc cttattttga ttttatatct ccaatttgcg
     8701 gatgtaattt tggcatcaga gttacgggcg ttgttgttgt aagccttttc tgttgaaata
     8761 tcgaatttat tgccgggctt tgataagctt attttttctc cgataacaaa tctttcattc
     8821 tcatctttag ggatattatt ctcatttaaa aactcaaaca agatatccga catgtcgggt
     8881 tgtggtgtgg caaagctcca cttttgatta tcctttcttt tatctttcga aatgacgtaa
     8941 agcctctttt caggacgtgt aaaagctaca tacaacagat ttagcatatc gagaaatgat
     9001 ttatccgatt ctctctgata gagttcctga taagaggtgt cttttagagc agaaatgagc
     9061 ggtattctgg aaaattttaa ttccggaata gatttgaacc ccggctcaat ccatgctgaa
     9121 ttttgagagt ttctcacgtt ctcaaacatg tatgggcaga ttaccacttt aaactgcagc
     9181 cctttagctt tgtgaatcgt catcacccgt accgaaggcg tatcttcggg gatgataatg
     9241 gattttttac aacccgtatc attccagaag tcgagaaaat cctgataaga cattggctgc
     9301 ttttgacttt gctcatatac catatctaaa aaaaaggtaa cgaaagggtc attatcacct
     9361 atatcgggtt gcaacgccat gataaaaaat tcgaaaatat cggtgagtga aagcgtatgc
     9421 atatggggct gggtgaggtc taatccaaag cctttcaggg tttcgttcat ggatattgac
     9481 gccggctcat cagggctaat ctctttgcat tgggcgagtg cctgatgaaa agaagcttct
     9541 ccaaatttgc ctcttttgaa aaaataaaac aaaatctccg ttattacttg gcgattctcc
     9601 tgttcgataa aaaattctaa taacgccatg agaactctca ccttaggact tgaaccaagt
     9661 aacagcgctt ctgacgacac cacagtgata ccttgctgaa gtaaataatt ggcaaccttg
     9721 atagcctgct tattttttct aaccaagata gcaatctctt gacgggcaaa gaggtttgtg
     9781 tcctctaatt cttttaatat ttcgaagaca cgtttctctc ttaccagttc acctacttcg
     9841 tcgttgttgt catcaacatt gtcttttaaa aattcaaact gtaaataacc cccactctta
     9901 tttaagttcc cctcttgttc caatccatca taaatacgct gtaagtcagg ggcaatttta
     9961 ctcttggcaa attcaaaaaa tgcattgtta aatctgatga tctctattgc tgatctgaaa
    10021 tttttatcca gatttttcaa tctgctttgg ctaacaaaga gtttttcacg agtaatgctt
    10081 gtttcgtcgt tacccctttt gtataattgt ggtaaagctg caaattgctc tacctcgcca
    10141 ttacgccagc gataaatagc ctgttttcca tcaccgacaa tcatgttgaa attgttggcg
    10201 ctaagagaat tttccaacaa ggggatcata ttctgccatt gcagcaggga tgtatcttga
    10261 aattcgtcta ttaaaaaatg gcggtatctt tcgcctattc gttcgtaaat atagggtatt
    10321 gcctctcttt gaacaatggc agcaatccgc ttattgaact cggatatgtg gacaatgttt
    10381 tcgttctgac gaaactcctc catcaccttt tcgatctccg aaagtatggc cattagataa
    10441 atattcctcc caaccaactg acttaaaagg tactcgtcat aatctttttc cagaatattc
    10501 tgaatctcat tataatcagt tatcaattca tccttgattg tggctattga atttttatca
    10561 gcagcgctac acgatgcaga agtccattta tcttcattaa ttgttttttg tatatagcta
    10621 ttaggcttaa tagtactaaa gtttccttcg gcaatttttt taaaataatt gccaattcct
    10681 tttagcccat gtgaaaaggc ttttaaatca attccttttt tgtctatcag gttactagct
    10741 ctttgtgcag ttttttttat tctattttca aagtcatttt tgatctttaa aagtttatgg
    10801 cttatctcta aaaaatcact tggcgaaagc ttagagatat tttttaatgc ttcgtaagtt
    10861 ctctcttcaa ttatattttg ggtaaaatct ttcagctctt ttttgatatt ccagtttttt
    10921 tcatcctcca ttttactgga gataaaactt attaatattt tagtaagatc cttatcaact
    10981 cctgctttac ttatgagtag atcaactgtt ttagcgatca gtttttctgt ttcaagttcg
    11041 acgtcaaagg cagccggcaa tcctaaatcc ctgctaaata ccctaacaag tttatgtaca
    11101 aaactatcaa tagtgcttac ggcaaatctg gagtaattgt gaagaatcag cgataaagcc
    11161 actgtagccc gtgaagaaat ttttcgagca tcaaaaccaa gctcctcctc aagggtggtc
    11221 atgagtgata ttattttatc atcaggctgt tcagtataaa atgaagagag ctccttcaag
    11281 ctacgcacaa tccgctcttt catctcattg gcagctttat tggtaaaagt gatggctaat
    11341 attttagaat acctttcagg attgctgatt accaacttta ggtactcttt tacaagagta
    11401 taggttttac cgcttcccgc ggatgattta aagacaacaa aattttttga ggttgtcgta
    11461 ggcataaatt attgaatgat caatctcttt gcagtagatt ttccgttcca ctccacttgt
    11521 atcagataaa ctccagaagc cggtttactg tcagatagat ttagcgtttg gaaatgttta
    11581 cctgctggca gattacctga aaagactctt ttaattttta taccagccat gttgataagc
    11641 gatattgtta tgtactccgg ttcgctaagt tctacaacaa taatagcttt atctattatt
    11701 ggatttggtg caatgtatat ttttggtttg cggatatcgg ctttttcaat tccaacccca
    11761 gggtcgaaaa tactatctat atatgtaacc tgcgtaattg cattctcttc ggtaacctct
    11821 aatagcggct gttttttacc gtttgtcaac catttgtatt cggtatattt acgcacaatt
    11881 tgctgtccga agttgagggt atcaacataa actgagtcgg tttccataac agatgatttt
    11941 aacctcaaca cgtcatattc gccgtaaggc gtaatgagtg ttccccagcc atcaacttcg
    12001 tttaaacgtt ttcgctgaat actaacatat ccaatatcag gcactcctaa ctgaccgctg
    12061 gcatcacttg aatcaatatt tccataagca agtgggagtt tataaatgac gtcagccgga
    12121 ttaagcctta gcggaacagg tatgccgcta atagttgctg cataaccgac atcattaaat
    12181 gcgttgcttg actttttata aaagcggtaa gggtcagtaa ttggcacctg ggggaagttt
    12241 tccagacccg tatatttctg cgctacattt gctacaatat tgggtatgaa aaccaattga
    12301 tataaaaacg gtaccgagct tacggtaaca aaagtatcaa cagtttgcga tgcaggtttc
    12361 aactgcgaaa agtcccataa gtagttttcc ccagttacac taaagtcaat acctgtaaaa
    12421 tccaatgcaa agctttttct cactgtatcg ccagcagatg gcatatcatt ttgtgtgata
    12481 gtgatttgcg agaaaacaga agtttgacaa gaaaataaaa taatgacgac aaggtaacta
    12541 aaagttgtcg gtaccctgaa attgaaaatg tatctcatgg aagtaggtat taaaatgtca
    12601 gaatgaatag gcaaaattaa aaaattcatc tttcaatttc gaaattatgc gcatcataat
    12661 ttaaattaaa cccgaaatta tctattgcag ataataattt gatgttactt cgttacaaac
    12721 tatcattatc ttttaaaagt cttaatgaat tgcaacaaac aattatactt ttgcaaaaaa
    12781 attgttattt cttttatctg taagtaagtt tttggtacaa gttctgcata aattcagcat
    12841 cattttaaca ccttaataat catcctgaaa cgaataatcc atatagtcat ctattttttg
    12901 gtcttttttg tgtcaatacc ggtagtcatt tcactggttt tgagtaatcc taccattcaa
    12961 acccttgttt cacgttatgc tgccgtatgg cttactcatc atattggtac cgaagttcat
    13021 attggtagta tcaatttggg tattcgtggc acattcctgt tgaaaaacct tatgattaaa
    13081 gataaatatg atgatgaaat gattagggtg ggagagttgg aaatattaat aacggggata
    13141 agtaataaga acaaagaggt aagtttgggg ctgttggagt taaaagattt tggatttcaa
    13201 cttcttaaac atcatgatgc ggaaatgacc aatttttcgt atttccttca aaactttgtt
    13261 tcaaaaacag ccgaagatac aaccgaagaa tccaaactgc catggagtat caaatgccgt
    13321 tcgataatta tttccaatgg ctactttaaa tatagtgatg atgacttttt aaccgaaaat
    13381 tataaaggga ttgattttaa cgatattgaa ttaaatgcca ggcagataaa aatcagcaag
    13441 ttccactttg accatgatac attaaaagcc aatataaaaa atatcgaact gattgagaag
    13501 agtgggttta agataaactc gtttgaggga ttgctaacct taaacccaac cttactcgaa
    13561 gtggatcagt tggtagctga aacaaacaat tccaaattaa atgcagatct taggtttgtc
    13621 ttaaacggct acgatgcttt cagcaatttc attcaagacg ttcagattga tggtttcttt
    13681 cggcctacaa taattaattt aagcgatata ggttatttcg aaccatcgct ttttccgatg
    13741 gttgacgaag ttgagttgaa aggtcagata gctggaacca ttgccgattt aaaggccaaa
    13801 aaacttgaat tcaattacgg caaaaacaca agttttattg gctcactcaa tataaagggg
    13861 ttgcccgaga ttgatactac attttttaat gccaacatcc aacattttac taccaatgca
    13921 aatgatttaa gatcattcaa ttttccgggt gataagcaaa aaattgaaat tccagagatt
    13981 gtcaaaaaac ttggaaatat caacatttca ggaacatttg ccggaaccta caatcaattt
    14041 cagtcgggat taaaggtaaa aagccagata ggctttatta gtgggcaatt acaagtaaac
    14101 caaagtgctg gcaaaaattt agtaagatat agcgggatgc tgttagctaa agattttgat
    14161 ctcggtaaac ttgttgattc agaacaactt ttaggcaaaa cttctttttc gttaaatatt
    14221 ttaactgcgt taatgggatc gcctacatat tt
//
LOCUS       DAOOAI010000059        13746 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_5757_length_13746_cov_5.580162, whole genome shotgun sequence.
ACCESSION   DAOOAI010000059 DAOOAI010000000
VERSION     DAOOAI010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13746)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13746)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13746
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5757_length_13746_cov_5.580162"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="PK345_05560"
     CDS             <1..242
                     /locus_tag="PK345_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="Ppx/GppA family phosphatase"
                     /protein_id="HPX34517.1"
                     /translation="TDQLQFAINHLRGFEIEDRIKSLGLKPDRADVILPAAEVFQFIT
                     NITGAESIYAPRLGLADGLIYQLFETYLANKRKKG"
     gene            complement(342..842)
                     /locus_tag="PK345_05565"
     CDS             complement(342..842)
                     /locus_tag="PK345_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX34518.1"
                     /translation="MVIKNFQDFHHNELYAIFRHIYLTSSFMSDDFDLRFNNSVSFKN
                     YYQQILDRPGSFVLIALIDEKPAGYLLLEPNPAMHLKHTALLNMGIIDIFRRQGIGVK
                     LVEAAFERARAEKIIEIIYLMVRSDNFAALQLYKKTGFETLTYLENDTKVGNEYFDGV
                     VMRKFL"
     gene            complement(1015..1629)
                     /gene="udk"
                     /locus_tag="PK345_05570"
     CDS             complement(1015..1629)
                     /gene="udk"
                     /locus_tag="PK345_05570"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005780344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HPX34519.1"
                     /translation="MFIIGITGGSGSGKTTVVKNIIHDLPKGSVAVISQDSYYKDNGH
                     LSDEEKKRINFDHPASIEFELLNKHIDKLIIGEPIAKPIYDYVTCARSTETIPIYPTK
                     VIIVEGILIFTNARLRNKFNVKIFVDADTDERLMRIIKRDLVERGRTIDEVLKHYETF
                     VKPMHLQFIEPTKRYADIIVPQGGANSVAIDILTSRIKMKLGEK"
     gene            complement(1705..3063)
                     /locus_tag="PK345_05575"
     CDS             complement(1705..3063)
                     /locus_tag="PK345_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HPX34520.1"
                     /translation="MKRFLTITGIIIFLTLISITFVNNGKSQPIVDSVQLSQDGEIVQ
                     NEGEEGQLDKSKGTQEVASQGIFKFLEYTGFANVTRGHLIMICFGLFFIFLGIRYEYE
                     PLLLIPIGTGILIGNVPFLLDAGLQVGIYEEGSVLNYLYFGVTAGIYPPLVFLGIGAM
                     TDFSSLISNPKLILLGAAAQLGIFATFLGALALGFHPAEAGAIGIIGGADGPTAIFLS
                     SKLANGINILPDGTTVKNLIGPIAIAAYSYMALVPVIQPPIMRLMTSNKERLIRMKPP
                     RAVAKLEKILFPIIGLLITTFISPSALPLLGMLFFGNLLKESGVTKRLADTARTSMVD
                     IVTIILGLTVGASTQADVFLTGESILIFVLGAISFMIATAGGVGFAKVMNLFLKDDNK
                     IHPFVGAAGVSAVPDSARVAEMEGLRYDPKNHLLMHAMGPNVAGVIGSAVAAGILLSY
                     LL"
     gene            complement(3103..3546)
                     /locus_tag="PK345_05580"
     CDS             complement(3103..3546)
                     /locus_tag="PK345_05580"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HPX34521.1"
                     /translation="MKKFTFSIEGHDYDIEIMDFEDQIAKVEVNGTVYSVKLHQEIKQ
                     SKTPKLVRKEVEIKRQDHKIKKTIVKTVGFEVKSPLPGDIIQIFVKEGDVVKLGDKLL
                     VYEAMKMENTILAEKDGTIKNLRVQQGDTVLQEETLMEIEAKVTA"
     gene            complement(3543..3914)
                     /locus_tag="PK345_05585"
     CDS             complement(3543..3914)
                     /locus_tag="PK345_05585"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family protein"
                     /protein_id="HPX34522.1"
                     /translation="MLLSRITFDLSLITNLGITISIVGYVIVFIALLILVWVFNSIPK
                     LLKVFVRWQLRKQGKPKDHEIMDLEGNIAAAIGLALHLHFKKSHDDESGIITIKRISR
                     QYSPWSSKIYSTNSFFRSGIL"
     gene            complement(3925..5484)
                     /locus_tag="PK345_05590"
     CDS             complement(3925..5484)
                     /locus_tag="PK345_05590"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HPX34523.1"
                     /translation="MDNQQKIQQLIELRKVARMGGGEKRIEAQHNKGKYTARERIEML
                     LDEGSFEEYDMFVTHRNTDFGMADKVYLTDGVITGQGTIDGRIVFIFSQDFTVFGGSL
                     SETYAQKICKVMDKAMKIGAPIIGINDSGGARIQEGVKSLAGYAEIFQRNILASGVVP
                     QISAIFGPCAGGAVYSPALTDVVIMSDKTSYMFVTGPKVTKTVTGETITEEDLGGATV
                     HATKSGVTHFRADDEDEGILLIRKVLEYLPQNNLEEPFVTVCNDPIDRLDDILNNIIP
                     DNPAEPYDVKDIIYTIVDDREFLEFHRHFAKNIVVGFAKFDGHPVGIVANQPNFLAGV
                     LDINASRKAARFVRLCDVFNVPIITLVDVPGFLPGSAQEYGGIIIHGAKLLFAYGEAT
                     VPKITITLRKSYGGAHDVMGSKQLRGDINYAWPSAEIAVMGPKGAIEILEGKTINEFT
                     SPDEAAKFIAEKEEEYRNKFANPYEAARYGYIDDVIEPRNTRFRIIRALQAISTKKDT
                     LPAKKHNNMPL"
     gene            complement(5515..5919)
                     /gene="mce"
                     /locus_tag="PK345_05595"
     CDS             complement(5515..5919)
                     /gene="mce"
                     /locus_tag="PK345_05595"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004584034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HPX34524.1"
                     /translation="MNLTHIEHIGIAVKSIEESLPYYENILGLKCYAIEEVKDQKVKT
                     AFFKVGQTKLELLESTDPEGPIGKFIEKRGEGIHHIAFAVDDVNKALNKAASKGIRLI
                     DEKPRKGAEGLNIGFLHPKFTQGVLTEFCGKE"
     gene            complement(5955..7130)
                     /locus_tag="PK345_05600"
     CDS             complement(5955..7130)
                     /locus_tag="PK345_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPX34525.1"
                     /translation="MAYPYTPPDYFLLDDLLTDEHKIVRSAIRDWVTRSVIPVIDEAA
                     QKHHFPRHFFKELGDIGAFGPFIPVEYGGAGMDYIAYGVIMTELERGDSGIRSAASVQ
                     TSLVMYPIHAFGSEEQRRKYLPKLATGEMIGCFGLTEPNHGSDPGSMETRFKDMGDHY
                     LLNGAKMWITNSTIADIAVVWAKDEQGVVRGLIVEKGMPGFTAPETFNKWSLRASVTG
                     ELVFEDVKVPKENLLPNIKGLKGALMCLNSARYGIAWGAIGAAMDCYNTALLYSLERK
                     QFHKPIASFQLQQKKLAEILTDITKAQFLAWRLGVLRNEDRATPQQISMAKRNNVEMA
                     LRTARESRQILGAMGITGDFPIMRHMMNLESVITYEGTHDVHLLITGADITGISAFS"
     gene            complement(7287..8132)
                     /gene="tatC"
                     /locus_tag="PK345_05605"
     CDS             complement(7287..8132)
                     /gene="tatC"
                     /locus_tag="PK345_05605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HPX34526.1"
                     /translation="MAKKRKDTEKEMTFWEHLEELRWHIVRSIAAIAIFAIIAFISKD
                     FIFNQIILAPKSGDFITHRGLCHIGRWLERHVAFISSSAFCLSDFNLKIINIKLAGQF
                     LMHFYISFFVGLIAAIPFVIREIWSFIKPALYENEKKYTGGAVLWSSMLFIAGVLFSY
                     FLIVPITILFLGTYNVSDYVENQIALNSYINTVVMLSFSVGLIFELPVFIYFLTKVGI
                     ITPAFMRKNRKIMIVVILIIAAIITPPDVFSQILIAIPLFGLYELSILISDRVFKKRE
                     KEIST"
     gene            complement(8154..9029)
                     /locus_tag="PK345_05610"
     CDS             complement(8154..9029)
                     /locus_tag="PK345_05610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34527.1"
                     /translation="MKVSGFTIIRNGQKFDYPFIESIQSMLNLCDEVIVAVGNSEDQT
                     LELVKAIDSPKIKIIQTVWDDNLREGGHVLAVETNKAKDAISPDTDWCIYLQADEVMH
                     EKDHPVIKIAMEENLANEKVDGLLFNHLNFYGSYDYIADSRRWTYKQIRVIRNNPAIR
                     SYRDAMSFKKNGKNLLVKKINATIYHYGWVKTPSAMQKKYETFQKLWHDDEWIEKNVQ
                     KVDEFDYSNIDSLFKFTGTHPSVMHERIKKMNWQFSFDPTKGIKLSLRLRILNWLEKK
                     WGLEIGKFKTYKIIR"
     gene            complement(9032..10141)
                     /locus_tag="PK345_05615"
     CDS             complement(9032..10141)
                     /locus_tag="PK345_05615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HPX34528.1"
                     /translation="MNLKQDKRIIMTLDAGGTNFVFSAIQAAEEVIESITLPASADSL
                     PELLKRIIEGFEQVRSKLATPPVAISFAFPGPADYENGIIGDLANLPLFRGGVALGPM
                     LKEHFKIPVFLNNDGDLFTYGEAIEGFLPYINSLLEENNSPKRFKNLLGATFGTGFGG
                     GIVTNGELFIGDNSAAGEINRIRNKIHPFYSAEETVSIRGIRRDYSRAANISEVLCPT
                     PQSIFEIGMGIVEGNKEAAKQAFKKFAIAAGDALANAATLVDGLIVLGGGIAGAHPLF
                     LQHLVHEMNLRFNTPDGKYLPRLEVKVFNLENEKELQSFIKGKKSEIKVPFSAEKITF
                     DPLQRIGVGISRLGTSKATAIGAYAFALNQLDKIP"
     gene            complement(10268..13057)
                     /gene="polA"
                     /locus_tag="PK345_05620"
     CDS             complement(10268..13057)
                     /gene="polA"
                     /locus_tag="PK345_05620"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002558586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HPX34529.1"
                     /translation="MPEPKKLFLLDAMALIYRAFYALNKNPRINSKGMNTSAVLGFAN
                     TLLDVIKNEKPTHLGVAFDTQAPTTRAEEYIEYKANRQAMPEDLANSLPYIVKLIEGF
                     NIPVLFVDGYEADDIIGTLVKKAEDKGFTSFMMTSDKDFGQLVSENTFIFRPAYMGNK
                     AETLGVKEVCERFEIERPEQVTDILGLWGDASDNIPGIPGIGEKTAKKLIATYGSLEN
                     VIAHVDDFKGKMAQNIREFAQQGIDSKRWATIILDVPIDLDEEALKLKLPDFDGLKVL
                     FEELEFKTYAKRVFDFYGSAEATTKPADNKLQKELFDATEFETPLNIKTIENTAHNYY
                     LLNTSEKISSLITKIKSEKSFCFDTETTGIDANSSELVGVSFSINPTEAFYLPVFENY
                     QKAFEQIRLFKEIFEDETIEKIGQNLKYDISILKWYEIDVKGKMFDTMLAHYLIEPDQ
                     RHNLDFMAEKYLNYRPISYKELIDKKEGKELTLREIQKVRPDDVLNYAAEDADITLQL
                     RYKLEPLLDDLGMKTLFNDIEMPLIPVLASMEAEGVKLDVEVLKKFSKQLESEIKEIE
                     NEIYALSGEEFNIGSPKQLGIVLFEKLKIDSKPKKTKTNQYSTSEDILTKLEHKHPIV
                     SKILDFRSLAKLKSTYVDALPELISMRDGRLHTSYNQAVTSTGRLSSTNPNLQNIPIR
                     TEKGREIRKAFVPRNDDFVLLAADYSQIELRIIAELSRDKEMMKAFIQGLDIHTATAA
                     RVYNVDIEKVTKEMRRNAKTVNFGIIYGISAFGLAERLNISRAEASEIIETYFKQYPG
                     IKQYMNDSIAFARKNGYVETMMGRRRYLNDINSANTNIRGFAERNAINTPIQGSSADM
                     IKIAMIRIYQQIEKRQMKSRMILQVHDELVFDAHKAEIDELKAIVEENMKNAFEMSVP
                     ILVDISTGNNWLEAY"
     gene            complement(13361..>13746)
                     /locus_tag="PK345_05625"
     CDS             complement(13361..>13746)
                     /locus_tag="PK345_05625"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008447183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HPX34530.1"
                     /translation="LIEWSSTGNPRKIIFADEREGKRVQDIWDYKDPQYPTYPTEKNP
                     DLLDLIILTSSNENSIVLDCFCGSGTTLKSAQLNGRKWIGIDKSDHAIKATINKLETI
                     EGDLFVAKPEYKFEELEKKQAANTA"
BASE COUNT         3960 a         2694 c         2570 g         4522 t
ORIGIN      
        1 cgaccgatca gctccaattt gcaataaatc atttaagagg atttgaaatt gaagatcgaa
       61 taaaaagttt aggtttaaaa cctgaccgtg cagatgttat tttacctgcc gctgaggtgt
      121 tccaattcat tacaaacatt acaggcgctg agtctattta cgctcctcgt ttgggacttg
      181 ctgatggatt gatttatcaa ctgtttgaaa catatttagc aaacaagaga aaaaagggtt
      241 agactgtgaa gccgataaat gctttaaact atcccgaaga gtagaaacag tttaatcaaa
      301 ttttacataa aatcgctcca atcaaaatgt aaaattatta cctaaagaaa tttccgcata
      361 actactccat caaaatattc atttccaact tttgtatcat tttcaagata agtcagtgtt
      421 tcaaaacctg tttttttgta aagttgtagg gcggcaaaat tatcgctgcg taccatcaaa
      481 tagattattt caattatctt ttcagctctt gctcgttcaa atgctgcctc aactaactta
      541 actccaattc cttgtctgcg aaaaatgtca atgataccca tatttaataa tgcggtatgt
      601 tttagatgca ttgctggatt gggttcgagc aagaggtatc ctgcaggttt ttcatcaatg
      661 agcgctatta acacaaaact acctggccga tctaatatct gttgatagta atttttgaac
      721 gagacagaat tgttaaatct taggtcaaag tcgtcgctca tgaaggagga ggtaagatat
      781 atatgccgga aaattgcata aagctcatta tggtgaaagt cttgaaagtt tttgatgacc
      841 attgcttcaa tagatgttag gaaaacattt tgtgcgttaa aacctaaaat acgagaaaac
      901 gagagatcaa agataattat atcttttttg ttgtgaagta gaagttttat cagtcctgat
      961 aataattgat atttcctctt attgtaacag agttattggg aggtttatca aattttactt
     1021 ttcacctaat ttcattttta ttcgtgaagt aagtatgtca attgccacgc tgtttgctcc
     1081 tccttgtggc acgatgatat cggcgtagcg ttttgttggt tcgataaatt gaagatgcat
     1141 tggttttaca aaggtttcgt agtgtttcaa tacttcgtca atggttctac cgcgttcaac
     1201 cagatcacgt tttataatac gcatcagtcg ttcatcggta tcggcatcaa caaagatttt
     1261 cacgttgaat ttgttgcgaa gtcgtgcatt tgtaaagatc agaatacctt ccactattat
     1321 aacttttgta gggtaaatag ggatagtttc cgtgctacga gcacatgtaa cataatcata
     1381 aatgggcttg gcaattggct cgccaataat gagtttatca atatgtttgt tcaacaattc
     1441 aaactctatt gatgccggat ggtcgaagtt aatacgtttt ttttcttcgt cggagaggtg
     1501 accgttgtct ttataatatg aatcttgaga tatgaccgct acagagcctt ttggcagatc
     1561 atgaattata tttttgacaa ccgtagtttt accagaacct gagcctccag taattcctat
     1621 tatgaacatt agatttacat ttttagctca aagataacct ttgttttcgg aaaacgatta
     1681 aaaacgaaag atcattctcg atttttataa aagatagcta agcaatatcc ctgctgcaac
     1741 tgctgaacca atgactccag cgacatttgg tcccatggca tgcattaaaa gatggttttt
     1801 cggatcgtat cttaaccctt ccatttctgc taccctagcg ctatcaggga cagcagatac
     1861 tcctgccgca ccaacaaatg gatggatttt attatcatct ttgagaaata gattcatcac
     1921 ttttgcaaaa cctacacctc cggctgttgc tatcataaat gatatagcgc ctaacacgaa
     1981 aatcaatatg gattcaccgg tcaggaatac atctgcttgc gtagatgcac ctactgttaa
     2041 tccaaggatt atagtaacaa tatcaaccat agaagtacgt gcagtatctg caagtcgttt
     2101 tgttactcca ctctctttca aaaggttccc aaaaaacagc attcccaata aaggcaacgc
     2161 acttggtgag ataaaggttg tgataagtaa gccgatgatg gggaaaagaa ttttttcgag
     2221 tttggcaacg gctcttggtg gtttcattcg gataaggcgc tccttgttag aagtcataag
     2281 gcgcataata ggcggctgaa taacgggtac taatgccata tacgaatacg ctgcaatggc
     2341 aatggggccg attaggtttt tcacagtagt tccatcaggc agtatgttaa taccgttggc
     2401 gagcttggat gataaaaaga tggctgttgg accgtcggca ccaccaataa tgcctattgc
     2461 gccggcttcg gcgggatgga aacctaatgc caatgctccc aaaaatgtgg cgaaaatccc
     2521 taattgtgcg gctgctccta aaagaattaa tttaggatta gaaataagcg atgagaagtc
     2581 ggtcattgcg ccaataccca aaaataccaa agggggatag ataccagcgg taacaccaaa
     2641 ataaagataa ttaagcacac tcccctcttc atatatgcct acttgaagtc ctgcatcaag
     2701 caggaaaggt acattgccaa ttaaaatacc agtacctatc ggaataagca aaagcggctc
     2761 atactcatag cgaattccaa ggaaaataaa aaacaaccca aagcagatca tgattaagtg
     2821 gcctcttgta acattcgcaa aaccggtata ttctaaaaat ttaaatatcc cttgtgaggc
     2881 aacttcttgg gttcccttag atttatccaa ttgaccttcc tccccttcat tttgtacgat
     2941 ttctccatcc tgggaaagct gtactgagtc aacaatgggt tggcttttac cattatttac
     3001 gaaggtaatg ctgattagtg tcagaaaaat tattatgcca gtgattgtta agaatctttt
     3061 cattttgatt tcctgttttg aaggtaaaaa gtacccaacg aattaagcag taacttttgc
     3121 ttcaatttcc ataagtgttt cttcttgcaa tacggtatct ccttgttgga ctctcagatt
     3181 ttttattgtc ccgtctttct cggcaagtat tgtattttcc attttcattg cctcgtacac
     3241 caacagctta tctcccaatt ttacaacatc tccttctttt acaaaaatct gaatgatatc
     3301 acctggtaga gggcttttta cttcaaaccc taccgtttta acaatagttt tcttaatttt
     3361 atgatcctga cgttttatct ctacctcctt tcgaacaagc ttgggagttt tgctttgttt
     3421 aatttcctgg tgaagtttta cggagtacac tgtaccatta acttccacct ttgctatttg
     3481 gtcttcgaaa tccatgattt ctatatcgta atcatggcct tctatcgaga atgtaaactt
     3541 tttcataata tgcctgatct aaaaaatgaa tttgtcgaat agatttttga actccaagga
     3601 gaatattgcc ttgaaattcg tttgattgta ataatgccgc tttcatcgtc atgtgatttt
     3661 ttaaaatgaa gatgaagcgc taaacctatt gctgctgcaa tatttccttc aagatccatg
     3721 atctcgtgat ccttaggttt accttgtttg cgtaattgcc atcgaacaaa cactttaagt
     3781 aattttggta ttgagttaaa cacccacact aaaattagaa gtgcaataaa aacaattaca
     3841 tatccaacta tagaaattgt aattcccaag ttggttataa gtgaaagatc gaatgttatt
     3901 ctgctcagta acatagcaat ttatttaaag tggcatgtta ttatgttttt tggctggcag
     3961 ggtatctttt tttgttgata tagcctgcaa ggcgcgtata attctaaatc gagtattccg
     4021 aggctcgatt acatcatcaa tatagccata tcgtgctgcc tcataagggt tggcaaattt
     4081 gtttctgtat tcctcttctt tttcagcaat aaatttggct gcttcatctg gcgaagtgaa
     4141 ttcatttatt gttttgcctt ctaaaatctc tattgcgcct tttggcccca ttacggctat
     4201 ttcggctgac ggccatgcat agttaatgtc gcctcttaat tgcttcgatc ccataacatc
     4261 atgtgctcca ccataagatt tcctaagggt aatagtgatc ttaggtactg tggcttcgcc
     4321 ataggcaaag agtaacttag ctccgtgaat aataatacca ccatattcct gtgcgctgcc
     4381 tggtaaaaag ccagggacat caacaagtgt gataatagga acgttaaaga catcacaaag
     4441 ccttacaaag cgggctgctt ttcttgaagc gttgatgtcg agaacacctg caagaaaatt
     4501 aggctgattg gctacaatac ctacaggatg accatcaaat ttcgcaaatc caactacaat
     4561 atttttggca aaatgacgat gaaactccag aaattcacga tcatcaacaa tggtgtagat
     4621 tatatcttta acatcataag gttcggcagg gttatctgga ataatgttgt taaggatatc
     4681 atctagcctg tcaatcggat cgttacatac tgtaacaaat ggttcttcaa gattattttg
     4741 gggtaggtat tcaagtactt ttctaatcag taagatacct tcgtcttcgt catcggctct
     4801 aaaatgtgta acacctgatt ttgttgcgtg aacggtagct ccgcctaagt cttcttccgt
     4861 tatagtttcc cccgtaacgg ttttggttac tttaggtccg gttacaaaca tgtagcttgt
     4921 tttatcactc ataataacta catctgtaag cgcgggcgag taaaccgcac cacctgcaca
     4981 aggtccgaat atcgctgaaa tttgaggtac cactcctgag gctagaatat tacgctgaaa
     5041 tatttcagca tagcctgcaa ggctttttac accttcctgt attctggctc cgccagagtc
     5101 gttaatgccg ataatcggag cgccaatttt catggcttta tccatcactt tacatatctt
     5161 ttgtgcataa gtttcggata acgatccgcc gaaaacggta aaatcctgag agaaaataaa
     5221 aactatcctt ccgtcaatag ttccttgccc tgtaattact ccatcggtaa gatacacttt
     5281 atcggccatg ccgaaatcgg tatttcggtg ggtaacgaac atatcatact cttcaaagct
     5341 cccctcatca agtaacattt cgatacgttc gcgggcagta tatttcccct tattatgctg
     5401 ggcttcaatg cgtttttcgc ctccacccat tcgagctacc ttacgtagtt caataagttg
     5461 ctgaattttt tgctgattat ccacgttagt ttccgtttat aaattgttta tagattattc
     5521 ttttccacaa aattcggtta agacaccttg ggtgaatttt ggatgtaaaa atccgatatt
     5581 taacccttca gcgccctttc tgggtttctc gtcaataagc cttattccct tgcttgctgc
     5641 cttattaaga gctttattta catcgtcaac agcgaaagcg atatggtgta tcccttcgcc
     5701 gcgtttttca atgaattttc caataggccc ttcaggatcg gttgattcta acaattcaag
     5761 cttggtttgg ccaaccttga aaaaagcagt ttttactttc tgatccttaa cctcttcaat
     5821 tgcatagcat ttcaatccta aaatattttc ataataagga aggctctctt caatactctt
     5881 tacagcaata ccgatatgtt ctatatgtgt cagattcatg atttaagata tttttttgct
     5941 ttttatcaat aaaattatga aaatgctgat attccggtga tatcggcgcc ggtaattaat
     6001 aagtgaacat cgtgagtgcc ttcgtaggtg ataacagatt cgaggttcat catgtgacgc
     6061 atgattggaa aatcgccggt tattcccatt gcacctaata tctgacgtga ttcgcgggcg
     6121 gtacgcaaag ccatttcaac attattgcgt tttgccatag atatttgttg aggagtagca
     6181 cgatcttcgt ttctcaacac tcccaagcgc caggcaagga attgagcttt ggtaatgtca
     6241 gtaaggattt cagcaagttt cttttgttgt aactggaatg atgcaattgg tttgtggaat
     6301 tgtttacgct cgagtgaata aagcagagct gtattataac aatccattgc agctccgatg
     6361 gctccccagg caattccgta acgtgcagag ttcaagcaca tcaatgcgcc ttttaaccct
     6421 ttaatattgg gtaataaatt ctctttaggc acttttacat cttcgaaaac aagttcaccg
     6481 gtaacggaag cccgcaacga ccatttgtta aaggtttcgg gagctgtaaa accaggcatc
     6541 cctttttcaa caataagtcc gcgtactacg ccctgttcat cttttgccca tacaacagca
     6601 atatcggcaa tggttgagtt tgtaatccac atctttgcac cattgagcaa atagtgatca
     6661 cccatatctt taaatcgtgt ttccatgctt ccggggtcag aaccatgatt aggttcggtc
     6721 aatccaaagc agccgatcat ttcaccggta gcaagcttcg gtaaatattt cctacgttgc
     6781 tcttcactac caaaagcatg gattgggtac attactaacg aagtttgcac tgaagctgcc
     6841 gacctgatac cggaatcacc gcgctcaagc tcggtcataa tgactccata tgcaatataa
     6901 tccataccgg cgccaccata ttctacaggg ataaaagggc caaaagcgcc aatatcgcca
     6961 agctctttga aaaaatgacg aggaaaatga tgtttttgag ctgcttcatc aattacaggt
     7021 attactgatc gtgttaccca gtcgcgaata gcggaacgaa caatcttatg ctcatcagtg
     7081 agcaagtcat ccaaaagaaa ataatctgga ggtgtgtaag gatatgccat tgataattat
     7141 ttaatatgat ttttgtgtaa ataatttcgg caaaagtact catattttga gctcaaaaac
     7201 tttttttgtt aaataaataa caaataaaaa taaattttca gattttatta taagtattat
     7261 aacaacactg gttttggaag tttgtattaa gtcgagattt ctttttcccg ttttttaaaa
     7321 acccgatcag atatcaaaat actaagttca tatagaccga ataagggtat agctattaaa
     7381 atctgactga aaacatctgg cggggtaatt attgcagcta tgatcaagat aacgacaatc
     7441 attattttac ggtttttcct cataaaggca ggcgttatta tgccaacttt tgtaaggaaa
     7501 tagatgaaaa cgggtagttc gaaaatcaac ccaaccgaga atgagagcat aacaacggta
     7561 ttgatatatg agttaagtgc tatttggttt tcaacataat cacttacgtt gtaggtgcca
     7621 aggaagagta tggtaatggg aacgattaaa aaatagctaa acaatacacc tgcaataaaa
     7681 agcattgaac tccacaacac agcgccaccg gtatattttt tttcattttc gtaaagagca
     7741 ggttttataa aactccatat ttccctgata acgaatggaa tggcagctat gaggccgaca
     7801 aaaaaggaga tgtaaaaatg cattaaaaac tgaccagcca gcttgatatt gataatcttc
     7861 agattaaaat cactaagaca aaaagctgaa gatgaaataa aagcaacatg tctttcgagc
     7921 caccttccaa tatggcaaag cccacggtgt gtaataaaat caccggattt gggagccaat
     7981 ataatttggt tgaaaataaa atccttgctg ataaaggcaa tgatggcaaa tatggctata
     8041 gcggcaattg aacgcacaat gtgccagcgc agctcttcaa gatgttccca aaaggtcatt
     8101 tccttctcgg tatctttcct tttcttagcc attgatggtg atgagttgaa tggttatctt
     8161 atgatcttat aagttttgaa tttcccaatc tccagtcccc attttttctc aagccaattt
     8221 aaaatcctca atcgtaatga tagcttaatc ccttttgtag gatcaaacga aaattgccag
     8281 ttcatctttt taattcgctc gtgcattact gatggatgcg ttcctgtaaa tttaaacaaa
     8341 gaatcaatat ttgaatagtc gaactcatca actttttgta catttttttc aatccattca
     8401 tcatcatgcc aaagcttttg aaaggtttca tatttttttt gcatggcaga gggggtcttt
     8461 acccatccat aatgataaat ggtggcatta atttttttca ccaacaggtt tttgccgttt
     8521 tttttaaaac tcattgcatc acgatatgag cgtattgcgg ggttgttacg gattacccta
     8581 atttgtttat atgtccaacg ccttgaatcg gcaatataat cgtatgagcc ataaaagttt
     8641 agatgattga aaagaagccc atcaactttt tcgttggcga ggttctcctc catggctatt
     8701 tttataaccg gatggtcttt ttcgtgcatc acttcgtcgg cttgaagata tatgcaccag
     8761 tctgtatcag gagagatagc atctttagct ttattagttt ctacagctaa gacgtgcccg
     8821 ccttcccgca gattatcatc ccatacggtt tgtataattt tgatttttgg cgaatcaatg
     8881 gctttaacca attcaagtgt ttgatcttca gaatttccta cggctacaat tacttcatca
     8941 caaagattaa gcattgattg aattgattca ataaacggat agtcaaactt ctgaccgttt
     9001 ctgataattg taaaccctga gaccttcatt attacggtat tttatcaagt tggtttaaag
     9061 caaatgcgta agctccgatt gcagtagctt tagaggtgcc aagtcggctg atgcccacac
     9121 cgattctttg aagtggatcg aaagtgattt tttcggcgga aaaaggaact tttatttcac
     9181 tttttttacc tttaataaaa ctctgcaact ctttttcgtt ctctaagtta aaaaccttaa
     9241 cctcaagtct gggtaaatat ttaccatccg gagtattgaa acgaagattc atctcgtgta
     9301 ccaaatgttg taaaaaaaga ggatgcgctc ctgcaattcc accacccaaa acaataaggc
     9361 catcaacaag tgtagctgcg ttagccaaag catctcctgc ggcgatggca aatttcttaa
     9421 aagcttgttt ggcagcctcc ttgttgccct ccactatgcc cattccaatt tcaaaaatgg
     9481 attgtggtgt gggacataac acttcggata tgttggcagc tctgctatag tcgcgtctta
     9541 tgcctctgat gcttactgtt tcttcggcgc tataaaaggg gtgtatttta tttcggattc
     9601 tgtttatttc gccggctgca gaattatccc ctatgaataa ctctccgtta gtaacgatcc
     9661 cgccgccgaa cccggttcca aaggtagctc ctaaaaggtt cttaaatctc ttggggctat
     9721 tattttcctc taaaagggag tttatgtatg gtaaaaaacc ttctattgct tcgccataag
     9781 tgaacagatc gccgtcgttg tttaggaata cgggaatttt aaaatgttct ttcagcattg
     9841 ggccaagcgc cacgccccct ctaaaaagcg gtaagttggc caaatcgccg ataattccat
     9901 tttcataatc cgcagggccc ggaaaggcaa aagatatagc tacgggcggc gtagctaatt
     9961 tgcttctcac ctgttcgaaa ccttctataa ttctttttaa taactcgggg agagaatcgg
    10021 cagaggcagg gagcgtgatt gattcaatta cttcttctgc cgcctgaatt gctgagaata
    10081 caaagttagt tccgccagca tcaagggtca ttattattct cttgtcttgc ttcaggttca
    10141 taagatgatt atttggtgta agagtgacag caaagatata gaaaaggttt taggtaagat
    10201 gaattttcat tctatcgtta taacagcata tggtttggaa aagtaatagg atttctaaag
    10261 aaagaactta ataagcctca agccagttat tacctgtact aatatcaacc aatatcggaa
    10321 ctgacatttc aaaagcattt ttcatgttct cttcgactat cgctttcaac tcatcaattt
    10381 cagctttgtg tgcatcaaat actaattcgt catgtacctg taatatcatt ctcgatttca
    10441 tttgtctttt ttcgatttgc tggtatatcc tgatcattgc aatttttatc atatcggctg
    10501 atgatccttg aattggagta ttgatcgcat tacgttcggc aaagcctcta atgttggtgt
    10561 tggctgaatt tatatcatta aggtatctgc ggcgccccat catagtttca acataaccgt
    10621 tttttcgggc gaaagcgatg ctgtcgttca tatattgttt gatgcctgga tattgtttga
    10681 aatatgtctc tatgatttcg gatgcttcag cgcgggaaat attaagtctt tcggccaatc
    10741 caaacgctga tatgccataa atgattccaa agttgacagt tttggcattt cgccgcatct
    10801 ctttagtaac cttttcgatg tcaacgttat aaactctggc tgccgttgct gtatgtatgt
    10861 cgagcccttg aataaacgcc ttcatcatct ctttgtcgcg gcttagttct gcaataatcc
    10921 gaagttcgat ttgtgaatag tcggcggcaa gtaaaacaaa atcatcgttg cgcggtacaa
    10981 aagcttttct gatctcccgt cctttttcgg tacggatggg tatattttgt aggtttggat
    11041 tggtagagct aagcctgcct gtggaagtta cagcctgatt atagctggta tgaagtcgcc
    11101 cgtcgcgcat actgataagt tcaggcaggg catcaacata agtagatttt agcttggcga
    11161 gtgaacggaa atcgaggatt tttgatacta tcgggtgctt atgttcgagt ttggtcaaaa
    11221 tatcttcact ggttgaatat tggtttgtct ttgtcttttt tggtttgctg tcaattttta
    11281 gcttttcgaa aagtacaata ccaagttgtt ttggcgatcc tatattaaac tcttctcccg
    11341 aaagtgcgta tatttcattc tcgatctcct taatttccga ttcaagttgc ttggaaaact
    11401 ttttcaaaac ctcgacatca agttttaccc cttcagcttc catagaggcc aatacgggaa
    11461 ttaatggcat ctcgatatcg ttaaaaagtg ttttcatgcc cagatcatcc agtagaggct
    11521 caagcttata tcgaagttgt agcgtgatat cggcatcttc ggcagcataa tttagcacat
    11581 catcaggtct tactttctga atttcgcgta aggttaactc tttgccctct tttttgtcaa
    11641 taagttcttt ataactaatc ggccggtagt taagatattt ttctgccatg aaatcaaggt
    11701 tgtggcgctg gtcgggttct attaaataat gtgcgagcat ggtatcgaac atctttcctt
    11761 ttacatcaat ctcataccat ttaaggattg agatatcata tttcaggttt tgaccgatct
    11821 tttctatagt ctcatcttca aaaatttctt tgaaaagtct tatttgctca aatgcctttt
    11881 gataattttc aaatacaggc agatagaatg cttcagtagg attaatagaa aacgacactc
    11941 cgacaagttc ggaactgtta gcatcgattc ctgtggtttc ggtatcgaaa caaaaagatt
    12001 tctcactttt aattttggtg atcaatgaag aaatcttttc ggaagtgttg aggaggtaat
    12061 aattatgtgc ggtgttttca atagttttaa tattgagggg tgtttcaaat tcagttgcat
    12121 caaacaactc tttttgtagc ttattgtcgg caggctttgt tgttgcttct gccgatccat
    12181 aaaaatcaaa taccctttta gcataagttt tgaattcgag ttcttcaaat aaaactttta
    12241 acccatcgaa atcaggtagt tttaatttca aagcctcctc gtccagatca atcgggacat
    12301 cgagaattat ggttgcccat cgtttggagt caatgccctg ttgggcaaac tcacgaatgt
    12361 tttgggccat tttcccttta aaatcatcaa catgtgcaat tacattttcc aatgacccat
    12421 aggtagctat taattttttt gcggtttttt cgccaatacc cggaatacca ggaatgttgt
    12481 cggatgcatc gccccaaagt ccaagtatgt cagttacctg ttcgggtctt tcaatttcaa
    12541 acctttcaca aacctcctta acacctaatg tttcggcctt attacccata taggctggtc
    12601 tgaagataaa tgtattttcg gaaaccaact ggccaaaatc tttgtctgac gtcatcatga
    12661 aagaggtaaa gcctttatct tcagcttttt ttaccaaagt cccaattata tcatcggctt
    12721 cgtagccatc tacaaaaagt acaggaatat taaatccttc aataagtttt actatgtaag
    12781 gcaatgagtt ggctaagtct tccggcattg cctgacgatt agctttgtat tcgatatatt
    12841 cttctgccct tgtggtgggg gcctgtgtgt caaaagctac tccgagatgt gtaggttttt
    12901 cattttttat aacatccagc agtgtgttag caaatccaag aactgccgat gtattcatcc
    12961 cttttgaatt gattcttggg tttttattta aagcataaaa agctctgtag atcagagcca
    13021 tggcgtcgag taaaaaaagt ttcttcggtt cgggcattat aaaaatctct taattattat
    13081 ttaataaaac ctttaacgga taaaatgaaa tttaattgat ccaaaccaga ttatttgtaa
    13141 aaaggatgcc tgttgttacg tgtctgaggc tgagcctctg taaccccata gaaaaaatgt
    13201 cattagtgta aaatatattt caaaaaagtg tcttttaaaa tgccctataa cgtttggcgt
    13261 gtaggtggag tagtgaatta gaggtgctta cctaatccat taagcacaaa ttttattaga
    13321 agcactaacc ttatgtttac aactttgcct gctattgctt ttaggcagtg ttggcagctt
    13381 gttttttttc taattcttca aatttatatt caggttttgc aacaaaaagg tctccttcaa
    13441 ttgtttcaag tttatttatt gttgctttta tagcatgatc tgatttgtca attccaatcc
    13501 attttcttcc atttaattgg gcagatttta aagttgttcc tgaaccgcaa aaacagtcaa
    13561 gtacaatgct attttcattg gaagaagtca gaataattaa atccaacaag tcaggattct
    13621 tttcagttgg ataggttgga tattgagggt ctttgtaatc ccaaatatct tgaactcttt
    13681 taccctctct ttcgtctgcg aaaattattt ttcttgggtt tcctgttgaa gaccattcaa
    13741 ttaatc
//
LOCUS       DAOOAI010000060        13655 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_5799_length_13655_cov_5.600441, whole genome shotgun sequence.
ACCESSION   DAOOAI010000060 DAOOAI010000000
VERSION     DAOOAI010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13655)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13655)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13655
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5799_length_13655_cov_5.600441"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            116..622
                     /locus_tag="PK345_05630"
     CDS             116..622
                     /locus_tag="PK345_05630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34531.1"
                     /translation="MEKQIEKAKYTIANPSKNKKLKFTISNRQVLGLNDKLIDKTRKL
                     LGIKGYFTNLKESALGNQTVIKRFHELYRIEQAFRISKNDLQIRPIFHVKEDPINLHL
                     LICIMALALSKHIELQTQISIKKFITESKKVTDARLLNTLTNKEIRIRTKISPDFIKI
                     IEKLNLSH"
     gene            complement(806..1744)
                     /locus_tag="PK345_05635"
     CDS             complement(806..1744)
                     /locus_tag="PK345_05635"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HPX34532.1"
                     /translation="MSAFTTIISFVFNGKIISINFSEPVAFKPSATVFEFLCTIENKN
                     SLIQEYARKDYEIFSVSLAQLDGRGGLEYKSFDPHLLLLPMMHGKQLIINRDELKIDP
                     PSDIYNFNAPEEEIIELLHSIADDRNTKILETMGLMYLKPFTLEEVLRLKHRYLDALV
                     INGLTHTRHLHIKNKMTLQRILDISDVAELNFMVEDKKFLAFGAGVNINEVITFCKRS
                     MVRLAETLQQVGSVQIRNQITLSDIITTAVLNGGLISFLTALGSRLRLASVIGNRECK
                     LEDCYSEHQKINIKPDELITLIIIPKLVDNEIIKSV"
     gene            complement(1806..2516)
                     /locus_tag="PK345_05640"
     CDS             complement(1806..2516)
                     /locus_tag="PK345_05640"
                     /inference="COORDINATES: protein motif:HMM:NF019284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HPX34533.1"
                     /translation="MKFPVILSSVLILAFSTTIYGQVSDDINKKDSAGLKQGFWRQTD
                     EEGRLKYEGQFKDDIPYGEFKRYDVNGKLKTISYISDNGRRSFTRSFHPNGNVMSEGL
                     YINQKKDSLWRFIDEAGNLLTTETYQNGIRNGEMKAYHPDGQIIEILNYTNDVKDGKW
                     QQYFMNGLPKLEAVYSEGVLSGHARYFYINGVVSSEGEYVNNLKSGEWRVYNEKGNLV
                     MIEIWENGIVKEKNALDN"
     gene            complement(2636..5785)
                     /locus_tag="PK345_05645"
     CDS             complement(2636..5785)
                     /locus_tag="PK345_05645"
                     /inference="COORDINATES: protein motif:HMM:NF015588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase family hydrolase"
                     /protein_id="HPX34534.1"
                     /translation="MSQSNFSAVIFDLDGVVTQTALVHSTAWKMMFDDFLKKWAKRNN
                     QPFIEFDHERDYLPYIDGKPRYQGVQSFLEHRGIGIPYGLPVDPPDAETVCGLGNRKN
                     KVFNEILQRDGVQVYSSTVKLIHQLKDRNIKIGLASSSKNSAAVLQAAGLLHLFETRV
                     DGVVSEKMGLKGKPAPDIFLTACKHLQCTPSKAVVVEDAVSGVQAGRNGNFGLVLGIA
                     REGNAKALKQSGADIVVGDLGEIELKDIEEWFCNGLEDDNWSLSYYDYDKTKERSREA
                     LLTIGNGYFGSRGAFEESVANPVNYPGTYVAGLYNRLVSRVAERDIENEDFVRIPDWL
                     QITFRIDGGDWFDPNKAEIVDCYRNLDFRNGILHRVITVKDSDGKETLIQSWRLASMN
                     DPHLAALKYTISPINYKGKITVRSTLNGNIINDGVDRYRQLNQKHLQSHNAGAENNIC
                     WLVTKTSQSGIQIAQAVRCDVFLENKRVLTTEESIIDKSEIHMLFSTQIKTFQVFTVE
                     KVAAIYTSKHDDSPNPLEDAKAKISETISFNQLVKDSKAEWKKIWSLIDINIQGDRLT
                     QKLLRLHLYHLMVSFSPHNQHFDASITARGLHGEAYRGHIFWDELFILPLYAMHFPEA
                     ARSMLMYRYRRLGKAREYAIEHGYKGAMFPWQSGSDGREETQIIHLNPVSGKWGDDYS
                     SLQRHVSLAIAFNIWNYLKITGDINFLRDYGAEMFFEICRFWASMVEKDRDGGRYSIK
                     NVMGPDEFHEAYPNSTEGGLKDNSYTNLMVAWMMSKAPDLLELLKPDDQALIKSKLLL
                     NDKELTKWEDISTKINIVVQNGIIAQYDGYFDLKELDWDFYKKKYGNVHRMDRLLKAE
                     GKSADEYKVAKQADTIMTFYNLTEEEVTQILKRLGYCLAEDYLKRNLDYYLGRTSHGS
                     TLSRVVHAYVSNKVGNKVDSWKLYKEALISDFTDIQGGTTGEGIHTGVMAGTVLVALR
                     SFAGVDIKNGMLYISPNMPEHWEEIRFNIGLSSDRIFFNVTAKKIELKMEGVSEKILV
                     KIKGENYNLFKEQKLIITF"
     gene            complement(5903..6838)
                     /locus_tag="PK345_05650"
     CDS             complement(5903..6838)
                     /locus_tag="PK345_05650"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPX34535.1"
                     /translation="MKKILKKELLAYLGMSVNTAIVGLSFIFMKMALRYTDPVVMLAH
                     RFSFAFLVLILMQLFGLVKFPSFSFSRIKPLLLLALFYPLMFFLLQTFGIQYSTASEA
                     GIIFAVIPVLTTLVASVFLKEKTNLLQKTGILMSVAGVFYIIFKTGIHKNDTDPLGVV
                     LLLLSVLSIIAYYTIGRKLGHTFSPVELTVLMSYLAFIIFNIWALSHHFLNGTPFKFI
                     DPLTHSGFLIPILYLGVLSSLLTSFLSNYALTAISASKIAVFNNLSPVIAVFGGVVFL
                     NEKLYGYHFIGGMLVLVGVVSTISVKSLNKPKSNI"
     gene            6973..7554
                     /locus_tag="PK345_05655"
     CDS             6973..7554
                     /locus_tag="PK345_05655"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HPX34536.1"
                     /translation="MAVNDTRDIILSVADRLFSRFGFHKTSMDEIAKTARKAKGSLYY
                     HFASKEELFREVISKEFGNLKAQLNPIVENKEYSAFEKLKNYLLKRMEILNSASNYHE
                     TLKADFFEQFHFIDELRSDLDEWEKEKFKTIVMEGVMAGEFKVSENIDVILDIFLMVL
                     KGLEIPFFLQGKYNEYAPHFEGLIYILNNGIAK"
     gene            7695..9959
                     /locus_tag="PK345_05660"
     CDS             7695..9959
                     /locus_tag="PK345_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HPX34537.1"
                     /translation="MAFQTKLAELIIRLKWLIIIIVVALSLFFGYGLRYITINPDIIS
                     SLPDGDQVAHLYKQIGNEFGGNDMGIIVLETDDVFTSEVLEGIRQITDSIQFTQGISY
                     VTSMTNIMNITSSEWGIEIGKLVDEYDLPSEQKELDELRDYVMSKEMYKGTIISDDGK
                     ATAIMFTLQPEGDKQSVAREIKTKIEEMKLPWKLYFGGLPLMMNDVNDLILNDIIWLI
                     PVVFIFIAGVLILSFQSLRGLLMPLLAAGIAILWTIGIMAYIGYELTIVTNITPVVLV
                     AVGSAYAIHVVNSINNSKTGDKKKTIVKALTYIIVPVILASSTTIVGFISFVFGSYLL
                     MIRDFGVFSALGTAIALLLSIFFVPALIAAFGKEQKIKSTTKKESKNTLIDRLLMPLV
                     HFVFNHPVRTLSIWGGVLIICIWGIFQIKTSINIVDYFKKNNPTRITENIMQEKFGGT
                     MPIFLVFEGDIQEPEILKMMMKTETYMKTDPNITIAQSVADLIEQMNDAMGEGLAIPD
                     EKEKIEQLWFLLDGQEIMEQLVNDDLSKGIIQAKFASIDSDEMETFIKKMEVFINENQ
                     SDNYIMSITGMPSIYVRLNESLIKSQYTSLLIAIAMVVLIVGSILKSLKKGVITAIPV
                     VTTVIILLGFMGITRITLDIATVLVASIVMGIGIDYSIHIMTGFNNFIIAGEGVDSAI
                     EKVLFFSGKAVVINVLSVSSGFIVLLFSQLVPLQNFGLLIAVSMIGSGLGALTILPAI
                     LILANRKKNSINNK"
     gene            9976..10710
                     /locus_tag="PK345_05665"
     CDS             9976..10710
                     /locus_tag="PK345_05665"
                     /inference="COORDINATES: protein motif:HMM:NF028441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA];
                     GO_process: GO:0044874 - lipoprotein localization to outer
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein-sorting protein"
                     /protein_id="HPX34538.1"
                     /translation="MKKVFRISILGLILFLVGDISLSAQSAADIVKKMDQVVYSPKNM
                     KGKNKIILIDKNGKEEVREAIVNQLGNNKRLMRFTAPASQAGISVLSLPNDVMYLYLP
                     AYGKERRIASSAKNQNFAGTDFCYDDMESVQFSDKYNSKMLSTEKDVFIIELTPKAKS
                     DYSKLIIHINKEHYYNEMIQYFDKAGNKFKEAHYHFTKVGNYWSASEIVMTDLKKNHT
                     TKMQMYDVQYDTNLTDDDFTVRKLVE"
     gene            10758..12134
                     /locus_tag="PK345_05670"
     CDS             10758..12134
                     /locus_tag="PK345_05670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34539.1"
                     /translation="MTNKIFALIVASLIIFSDSTLAQNENSLGFTGNLRTDQRFLLKD
                     KNDWAWNENRLTINLDKPFSNQVRFHSEMWIRNFGLPSLYSINDLYNKGIVDPYQFEI
                     REAYIRINNFPGKKFDLTLGRQHINWGTADKLNPTNNLNPYDLEDILDFGRQRGTEAL
                     SMNYYFNNDFSVQVALFPFFRPANMPVGIFSGLLNPKMELPGNLYIKSFSDSLIMPRL
                     NLKENLTGGLRFKGFAAGIDFSLSYALAYDGLPVPLKNSFERLGFDGATKLRSELIFY
                     RNHIIGADLATTLGGCGIWAEAAMFIPEKDVIMTNKFAAGLIVPFPFSVDSLYIDKTK
                     PFARFIVGGDYMFADGSYLNLQYLHGFIHERRVKELNDYFFMRYDRSFFREKLKISPL
                     SGGFIVTDWNSLKENYAIVYIPEVVYMVTPNVETSISAVISDGKGDNLFLGLKDLNMM
                     MVKIKYSF"
     gene            complement(12631..>13655)
                     /locus_tag="PK345_05675"
     CDS             complement(12631..>13655)
                     /locus_tag="PK345_05675"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPX34540.1"
                     /translation="IYHQVVSVLEDFFFIEFGGIEPNPQYSTLMKAVDLCRKEKIDFL
                     LSVGGGSVLDGTKFIAAASLFDGEPWDILENQLKVTSVIPFGAVLTLPATGSEMNAFA
                     VISRKETGQKLDFGTPPFTYPRFSVLDPNFTRTLPHSQRINGVIDAFVHVTEQYLTFQ
                     QNAPLNDRFAEAIMKTLIEEGLNYVNEPENMTVAKNIMWCSTMALNGLIATGLITDWA
                     THKIGHELTVLHNIDHASTLAIVWPGMMRVMSQIKKEKLLQYAERIWDINNGDDEERI
                     EKAIFKTEAFFISLGAKTRLSDYKLNKTTIVQVVENLKRNGFVAIGEQKLVTPEKVIA
                     ILESRM"
BASE COUNT         4392 a         2629 c         2467 g         4167 t
ORIGIN      
        1 taattgaata tctacagttt atttttttca ataagaaaaa ggggacaagt caggagacaa
       61 tggttctttg atatgcaggt attcttcggc ccgctaccga aaagataaat atgaaatgga
      121 aaaacagatt gaaaaggcca aatacacaat agcaaaccca tcgaagaaca aaaaattaaa
      181 atttaccata tcaaacaggc aagtccttgg gctcaatgat aaactcattg ataaaacccg
      241 aaaactacta gggatcaaag gttattttac caatttgaaa gagagtgccc ttggcaatca
      301 aaccgttatt aaacggtttc atgaacttta caggattgag caagcattca ggatatcaaa
      361 gaacgacctg cagataaggc ccatatttca tgttaaagaa gaccccatca acctgcacct
      421 attaatttgc ataatggcct tggcattatc aaagcatatt gagcttcaaa cccaaatatc
      481 gatcaaaaaa tttataactg aatcaaaaaa ggttaccgat gcaaggctgc tcaatacatt
      541 aaccaataaa gagatcagga taagaaccaa aatatcaccc gattttataa aaataatcga
      601 aaaactaaat ctgtcgcatt aaagtggaca agacaggata aattaatcga aaaacagaca
      661 attgcagatt ccgtattaaa tagaatagtt aattcttcgc atagaataaa aattaaacaa
      721 gcatcagtta tcatgataaa ttctttgtaa ttacgactga cacagacaat taatagtctg
      781 tcaaactagt ttaactaccc attccttaaa cagattttat aatctcgtta tctactaatt
      841 ttggaatgat aattagtgta attaactcat caggttttat gttaattttt tgatgctctg
      901 aatagcaatc ttcaagttta cattcgcgat ttccaataac acttgcaagc ctaagccggc
      961 ttcccaaagc agtaagaaag gaaatcaatc ctccatttag aacagccgtt gtaatgatat
     1021 cacttaaagt aatctgattt ctaatctgca ccgatccgac ctgctgcaag gtttcggcta
     1081 gccgaaccat actcctttta caaaaggtga ttacctcatt tatatttact cccgctccga
     1141 aagcaagaaa ctttttatct tcaaccataa agtttaattc tgcaacatca gaaatatcga
     1201 ggattctctg caaagtcatc ttatttttaa tatgtaaatg acgggtgtgt gtcaaaccat
     1261 tgataaccaa agcatccaga tacctgtgtt taaggcgtag aacctcttca agagtaaatg
     1321 gctttagata catcagaccc atggtctcca atattttcgt gttcctgtca tctgctatgc
     1381 tatgcaaaag ttctataatc tcttcttctg gagcattaaa attgtaaata tcggacggtg
     1441 ggtctatttt aagttcgtct ctgttgatta tcagttgctt gccgtgcatc atcggcagta
     1501 gaagtaaatg cggatcaaaa ctcttatatt ccaatccccc tctaccatcc agttgagcca
     1561 gtgaaacaga aaaaatttcg taatccttcc tggcatactc ttgtatcaag ctgtttttgt
     1621 tctcaatcgt acaaagaaat tcaaatactg tagcagatgg cttaaaagct actggttcag
     1681 aaaaattaat cgagattatt tttccattaa aaacaaatga aataatggtg gtgaaagcgc
     1741 tcatttaata tcgtggtgca aaacaaaccg atagtaagtc aacaaagata attctcccgg
     1801 agtagttaat tatccaaagc atttttctct ttaacaattc cattttccca tatttcgatc
     1861 atcactaaat ttcccttttc gttataaacc ctccactcgc ctgacttcag attatttacg
     1921 tattcgccct ctgagcttac aactccatta atataaaaat atctggcatg acctgacaat
     1981 accccttcgc tgtaaacagc ttcaagttta ggcagtccgt tcataaaata ctgctgccat
     2041 ttaccatctt ttacgtcatt tgtataattg agtatttcaa taatctggcc atcaggatgg
     2101 taggctttca tttcaccatt tctgattcca ttttgatagg tttctgtagt taacaaatta
     2161 cccgcttcgt caataaatct ccataggcta tctttttttt gattgatata aagcccttcg
     2221 ctcatcacat ttccgttagg atgaaaggag cgagtaaacg aacgtcttcc attatcagag
     2281 atataggata tggtttttag ctttccattt acgtcgtatc gcttaaactc gccataagga
     2341 atatcatctt taaactggcc ttcgtacttc aatcttcctt cttcgtcagt ttgtcgccaa
     2401 aaaccctgtt tcagccctgc cgaatctttt ttattgatat catcagaaac ctgaccatat
     2461 atggttgttg aaaaagccaa aatcaaaact gaacttaaga ttacgggaaa tttcatattt
     2521 tgaaaatttt ctatctgtaa cgcaaaatac aaagtaatat ttaggagcat aataagtgat
     2581 acagcacatt aaaaaaagta cttcctgttg attatttagt tttaaaagag cttgcttaga
     2641 aggttataat cagcttctgc tctttaaaca ggttgtagtt ctctcctttt atttttacaa
     2701 ggattttttc tgagacccct tccattttca attcaatctt tttagcggtt acattaaaaa
     2761 atatcctgtc ggaacttagt ccgatattaa acctaatttc ttcccaatgt tcgggcatat
     2821 tgggagagat atacaacatt ccatttttga tatctactcc agcgaaagat cgtaaagcta
     2881 ctaaaacggt accggccata acacctgtat gaattccttc gccggttgtg cctccctgta
     2941 tatcggtaaa gtcgctgata agcgcctcct tatacagttt ccagctatcc accttattgc
     3001 caaccttatt actaacataa gcatgaacca ctcttgagag tgttgagcca tgcgaggttc
     3061 gcccaaggta ataatcaaga ttgcgcttca aataatcctc cgccaagcaa tatcctaatc
     3121 gtttaagaat ttgtgtaacc tcctcttcgg tcaagttgta aaaggtcatt atcgtgtcgg
     3181 cctgtttagc gactttatat tcatcagccg atttgccttc agctttcaac agccgatcca
     3241 tcctatgcac attaccatat tttttcttgt aaaaatccca gtcaagctcc tttaagtcaa
     3301 aatatccatc atattgggca atgataccat tctgaacaac gatatttatt tttgtggaga
     3361 tatcttccca ctttgtcagc tccttatcgt tgagaagcaa cttcgatttt atcagagctt
     3421 gatcgtcagg tttcagtagt tccaataaat cgggagcttt gctcatcatc caggctacca
     3481 tgaggttggt ataggagtta tctttcaacc cgccttctgt tgagttggga tatgcttcgt
     3541 gaaactcatc aggtcccata acatttttaa tagagtatct gcctccatca cgatcttttt
     3601 ccaccattga tgcccagaat cggcatatct caaaaaacat ctcagcgccg taatctctaa
     3661 gaaaatttat atctccggta atcttcagat aattccatat attaaaagct atggctaatg
     3721 acacatgtcg ttgcaatgag ctataatcat ccccccattt tcccgaaaca gggttaagat
     3781 gaattatttg agtctcttct cttccatcgc taccgctttg ccagggaaac attgctccct
     3841 tataaccgtg ttcgatcgca tattcacggg ctttgccaag cctccgatag cgatacatca
     3901 gcatggagcg agcagcttcg ggaaagtgca tagcatataa tggcagaata aaaagctcgt
     3961 cccaaaaaat atgtccgcga taagcctctc cgtgtaagcc tcttgctgtt atgctcgcat
     4021 caaaatgctg attgtgcggc gaaaaagaga ccattaaatg gtaaagatgc agtcgtaaaa
     4081 gtttttgtgt cagcctgtcg ccttgaatat taatatcaat cagactccat atttttttcc
     4141 attctgcttt actatcttta acaagttgat taaacgagat cgtttcgctg atctttgcct
     4201 tggcatcttc aagcggattg ggcgaatcgt cgtgtttaga ggtgtaaatt gctgcgacct
     4261 tctcaactgt aaaaacctga aatgttttaa tctgcgtact aaaaagcata tgaatttcac
     4321 ttttatctat gatgctctct tctgtagtca aaacacgttt gttttcaagg aaaacatcgc
     4381 atctcacagc ctgtgctatt tgaatgcctg attgagatgt cttggtaacc aaccagcaaa
     4441 tattattttc agcgccggca ttgtgtgatt gcaaatgttt ttgatttaac tgccggtaac
     4501 gatccactcc atcgttaata atattaccat taagggtgga tctcacagta attttccctt
     4561 tatagttaat aggtgaaata gtatatttta acgctgcgag atgcggatca ttcatggatg
     4621 ctaagcgcca cgactggatc agggtttcct taccatcact atctttaact gtaattacac
     4681 gatgcaatat cccattacga aagtctaagt tacggtaaca atccactatt tcagctttgt
     4741 tagggtcgaa ccaatctccc ccatctattc gaaaggtgat ctgtagccaa tctggtattc
     4801 tgacaaaatc ttcattttct atatcgcgtt cagcgactct tgaaacaagc cggttgtaga
     4861 gacctgcaac ataagtccct ggataattta ccggattggc tactgattct tcgaaagccc
     4921 cacggctgcc gaaataacca ttacctattg tcaataatgc ttcgcgcgaa cgctcttttg
     4981 tcttatcata gtcgtaatag ctaagcgacc agttgtcatc ctcaaggccg ttacaaaacc
     5041 actcttcgat atcttttaac tcaatttcgc ccaggtcgcc cacaactatg tcagcgccac
     5101 tttgtttcaa cgcttttgcg tttccttcgc gtgcgattcc caataccaat ccaaagttac
     5161 cattccgacc ggcctgtaca cccgaaactg cgtcttcaac aactacagct tttgatggtg
     5221 tacattgcaa atgtttacaa gccgttaaaa aaatatcggg agccggctta ccttttaatc
     5281 ccattttttc agaaacaacc ccatcaacac gtgtttcgaa aagatgaagc aagccggcag
     5341 cctgtaatac agccgcgcta tttttacttg aagaagctaa gccaatcttt atatttctat
     5401 ctttcaactg atggattagc tttacagtag atgaatacac ctgtacgcca tcacgctgta
     5461 atatctcatt gaatactttg tttttccggt tacccaaccc acataccgtc tccgcatcag
     5521 gtggatcaac gggtaaacca taagggatgc caatacccct gtgttccaaa aacgactgaa
     5581 caccttgata acgaggttta ccatcaatat agggaagata atcgcgttca tgatcgaact
     5641 ctatgaatgg ctgattgttg cgtttagccc atttttttaa aaaatcatcg aacatcattt
     5701 tccatgctgt gctatgcact aaagccgttt gagttaccac gccatcaagg tcgaaaatta
     5761 cggctgaaaa gttactttga gacatacact ttaagtattt aatcagatta tgtcaataaa
     5821 aaaagcaaaa gtagaaattt tgagtcttgc tggctttgca gccaattact atcaacatta
     5881 tcttaatcgt ctatacattt ggtcaaatgt ttgatttagg tttattaagg cttttgacag
     5941 aaatagtgga aactacaccg actaaaacaa gcataccgcc aataaaatga tagccatata
     6001 acttttcatt tagaaaaact acacctccaa aaactgcaat cactgggctt agattgttga
     6061 aaaccgctat tttggatgct gaaatagccg ttagcgcata gttggagagg aaagaggtaa
     6121 gcagcgaaga aagaacccca agatacaata taggtatcaa gaagccggaa tgagtcagcg
     6181 ggtcaataaa tttgaaagga gtgccattta aaaaatgatg agagagcgcc caaatgttga
     6241 aaatgataaa agcaagataa ctcatcaata ctgtaagttc aaccggcgaa aatgtatggc
     6301 cgagttttct tccgatagtg taataggcta taatagacaa aacggaaagc aaaagtaata
     6361 cgacccctaa gggatcggtg tcgtttttgt ggataccggt tttaaagata atgtaaaaca
     6421 caccggctac agacattaag atgccggttt tttgcaataa attcgttttc tctttcaaaa
     6481 aaaccgaagc tacaagggta gttaacaccg gaatcacagc aaaaataatt cccgcctcag
     6541 aagcagtact atactgtatt ccaaaagttt gtaacagaaa aaacatgagc gggtaaaaca
     6601 atgccaaaag taaaaggggc ttgatccttg aaaaactgaa actcgggaac ttaaccaagc
     6661 caaacaactg catcaaaata agtacaagaa aagcaaatga aaaccgatgc gccagcataa
     6721 caaccggatc ggtatatcgg agagccatct tcataaaaat aaaagataaa cctacaatcg
     6781 cagtgtttac cgacatgcca agataggcca acaactcttt tttcaatatt tttttcatgc
     6841 tgatcgcaaa tgtacaaaag agaaataaaa gcgataggca atggttgata caataaaatt
     6901 taaaaagaaa tgttttttta aactaaaact attacttttg taccgaaaaa cgtttttatt
     6961 acaaaatgtt cgatggctgt taacgatacc cgcgatataa ttttgtctgt tgctgatagg
     7021 cttttcagcc gttttgggtt ccataaaact tcgatggacg agattgctaa aactgcacga
     7081 aaagccaaag ggtcgctcta ctaccatttt gcaagcaaag aggagttatt cagagaggta
     7141 atttctaaag agtttggaaa tctgaaagcc caactaaacc ctattgttga aaataaagaa
     7201 tacagcgctt ttgaaaaact taaaaactat ttgctcaaac ggatggagat tttaaactca
     7261 gcctcaaact accacgaaac ccttaaagct gactttttcg aacaattcca ttttattgat
     7321 gagttaagaa gtgatttaga tgaatgggaa aaagaaaaat ttaaaacaat tgtgatggaa
     7381 ggtgtcatgg ctggagaatt taaggttagc gaaaatattg atgtaatact tgatatcttt
     7441 ttaatggttt tgaaggggct tgaaattcca ttttttctgc aaggtaaata caatgaatat
     7501 gcgccgcatt tcgaaggatt gatttacatt ttaaacaatg gaatagcaaa ataatttttt
     7561 ttaattaaaa ctgactataa aacattaata gtacaaatat tataatttta atcattaata
     7621 aacagggatt tagccctttt aattttttac gaatttgact ttaaaacatt aatagtatag
     7681 atattacaaa ttttatggca ttccaaacca aattagcaga actaattatc cgcctgaagt
     7741 ggctgattat tattattgta gttgcgctga gccttttttt cggatatggg cttaggtata
     7801 ttactatcaa tccagatatt atctcttcat tacctgatgg tgatcaagtg gctcatcttt
     7861 acaagcaaat aggtaatgaa tttggtggta acgacatggg aattatcgtt ctagaaaccg
     7921 atgatgtttt cacttcagag gttcttgaag gtatccgaca aataaccgac agtatccagt
     7981 ttactcaggg gatttcctat gtaacaagta tgaccaacat tatgaatatt accagttcgg
     8041 aatggggtat cgaaatcggg aaattggttg acgaatatga tctccccagc gagcaaaaag
     8101 agctggatga actacgggat tatgtcatgt cgaaagagat gtacaaagga actatcattt
     8161 ctgatgatgg aaaagccaca gcaatcatgt ttactttgca gcccgagggg gataaacaat
     8221 ctgttgcccg agagattaaa acaaaaattg aagagatgaa gctgccctgg aaactttatt
     8281 tcggcgggct ccccttaatg atgaacgatg taaacgacct gatacttaat gatataatat
     8341 ggttgatccc tgtggtattt atatttattg caggagtttt gattcttagc ttccaatcac
     8401 tcagaggatt gcttatgcca ttgcttgcag caggaatcgc catactttgg acaataggaa
     8461 ttatggccta cataggatac gagcttacca ttgttaccaa tattacgcca gttgttctgg
     8521 tggcagttgg aagcgcatat gccatccatg tggttaacag tataaacaac tcaaaaacag
     8581 gagataagaa aaagacaatt gtcaaagcgc tgacttatat cattgtgccg gtaatccttg
     8641 catcaagcac tacaattgtg ggatttattt cttttgtttt tggatcttat ctcctaatga
     8701 tcagagactt tggggtattt tccgcgcttg gaacagccat agcattacta ctttcaatct
     8761 tttttgtacc agcgctaatt gctgcatttg gaaaagaaca aaaaataaaa tccacaacaa
     8821 aaaaagagtc taaaaacacc ttgatagaca gacttttgat gccgcttgtg cattttgtgt
     8881 tcaaccatcc ggtcagaaca ttaagtatat ggggaggtgt attaataata tgtatctggg
     8941 gcattttcca aataaaaacc agcatcaaca ttgttgatta ttttaaaaag aacaacccca
     9001 ctcgcatcac cgaaaacatt atgcaggaaa aatttggtgg caccatgccg atatttttgg
     9061 tttttgaagg tgatattcag gagccggaaa ttctaaaaat gatgatgaaa acagagacct
     9121 acatgaaaac cgatccaaat atcactattg ctcaatccgt tgccgaccta atagaacaaa
     9181 tgaacgatgc catgggcgaa gggcttgcta ttccggatga aaaagaaaaa attgagcaat
     9241 tatggttttt acttgacggg caagaaatta tggaacaact tgtgaacgat gacctgtcga
     9301 aaggaattat tcaggccaag tttgcatcta ttgatagtga tgagatggag acattcatca
     9361 aaaaaatgga ggtctttatc aatgaaaatc aaagcgataa ttatataatg agcatcaccg
     9421 gaatgccatc tatttatgta cgactgaacg aaagcctgat aaaaagccag tacacaagcc
     9481 ttttgattgc tattgcaatg gtggtgctga ttgtaggatc aattctgaaa tcactaaaaa
     9541 aaggagtaat tacagctata ccggtagtta ccacagtaat tattttattg ggctttatgg
     9601 gaattaccag gatcaccttg gatatagcta ccgtattggt tgcaagcatt gtaatgggta
     9661 ttggtataga ttattccatt catattatga ccggcttcaa taatttcatt attgctgggg
     9721 agggtgtaga ttcggctatt gaaaaagtgt tattcttcag tggaaaagct gtggtaatca
     9781 atgtattatc cgtttcgtcc gggtttattg tgctgctctt ctcacagctt gtacctcttc
     9841 agaactttgg acttcttatc gcagtaagta tgataggctc aggtttaggc gctctcacca
     9901 tactgccggc gatcttaatt ttagcaaaca gaaaaaaaaa ttcaattaat aataaataaa
     9961 ataaaagtaa aaatcatgaa aaaagtattt agaatcagta tcttaggatt aattcttttc
    10021 ttagtaggag atataagcct gtcggcacaa tcggctgccg atattgtaaa aaaaatggac
    10081 caggttgtct attcacccaa aaatatgaag gggaaaaaca aaatcatcct tattgataaa
    10141 aacggcaagg aggaagtgcg cgaagccatt gtaaatcaat tgggtaataa caagcgtttg
    10201 atgcgtttca cagcgccggc ctcacaagca ggcatttctg tcctctcgct acccaatgat
    10261 gtaatgtatt tatacctacc cgcttacggg aaggaaagaa gaattgcatc gagcgcaaaa
    10321 aaccaaaatt ttgccggaac tgacttctgt tatgatgata tggagtctgt ccagttttcg
    10381 gataagtata attcaaaaat gcttagtacg gaaaaggatg ttttcataat cgaactgacg
    10441 ccaaaagcaa aatcagatta ttcaaaattg atcattcaca ttaataagga acactattac
    10501 aatgaaatga tccagtattt cgacaaggcc ggaaacaaat ttaaagaggc tcattaccat
    10561 tttaccaaag ttggaaatta ctggagtgca tcagaaatag tgatgaccga tttgaaaaaa
    10621 aatcacacta cgaaaatgca aatgtacgat gttcaatatg ataccaatct gactgatgat
    10681 gattttactg tgagaaaatt ggttgaataa acagtgaata atgtttcatt ttttgcttca
    10741 tttcaattaa aaatgatatg accaataaaa tttttgctct aatagtagcc tctctaataa
    10801 tattttccga ctctacatta gcccaaaatg aaaacagcct tggatttacc ggcaatttac
    10861 gtaccgacca acgctttttg ctaaaagata aaaacgattg ggcatggaat gaaaatcgct
    10921 taactattaa tcttgataag cccttttcaa atcaggttcg ttttcatagc gaaatgtgga
    10981 ttcgtaattt tgggttacca tcgctatatt caatcaacga cctgtataac aaaggaattg
    11041 ttgatcctta tcagtttgag atcagggaag cttatattcg tataaataat tttccgggca
    11101 aaaagtttga tcttactttg ggtaggcaac acatcaactg ggggactgct gataagctca
    11161 atccaacaaa taacctgaac ccttacgatc tggaagacat actcgacttc ggtcgccagc
    11221 gtggaacaga agcgttaagt atgaattatt acttcaataa tgatttttca gttcaggtag
    11281 cgctttttcc tttttttcgt ccggcaaata tgcctgtcgg gattttttca ggtctgttga
    11341 atccaaagat ggaactgccg ggaaatcttt atataaaaag cttttccgac tccctgatca
    11401 tgcctcgcct taatctgaaa gagaatctaa caggtggatt gcgttttaaa ggttttgctg
    11461 ccggtattga cttctctctc agttatgcct tggcttacga cggtcttccc gttcctttaa
    11521 aaaattcatt cgagcggctt ggctttgacg gggccactaa acttcggtcg gaacttattt
    11581 tttatcggaa tcatatcatt ggcgccgatc tggctacaac tttaggtgga tgtggcattt
    11641 gggctgaagc ggcaatgttc atacccgaaa aagatgtgat aatgacaaat aagtttgcag
    11701 ccggattaat agttccgttt ccgtttagcg tagattcact gtacattgac aaaactaaac
    11761 cctttgctcg ttttattgta ggtggcgatt atatgtttgc ggatggatcg tacctgaatt
    11821 tgcagtatct ccatggattt attcacgaaa gaagagtaaa ggagttgaat gactactttt
    11881 ttatgcgata tgaccgaagc ttttttaggg aaaaattaaa gataagccca ctttcaggag
    11941 gctttatcgt aactgactgg aatagcctaa aagaaaacta cgccatcgtt tatatccccg
    12001 aagttgttta tatggttact ccaaatgtgg aaacgagtat ttctgcagtt atttcagatg
    12061 gaaaaggcga taatcttttt ttgggattaa aagacctgaa catgatgatg gtaaaaataa
    12121 aatacagctt ttgacgggaa ttaaaagata aatcggaaaa aaaggtgaca cagaggtcac
    12181 cttttttatt taaaatccag aacgccaaat aatatgagag tcaattgttt tatcttataa
    12241 aaaaatatga aaagacaagt tggacctttc atttggtaaa aaagaaaaaa ttaataaaac
    12301 gagcaatttt aaaaattata tggagaggca aagcaaaaaa actacgctgg aaatatggaa
    12361 ttaaataccc aagccgcgaa ggtatatttg taatttttgg ctatcctgct gttgagtata
    12421 aaaaggggtt gaaaaggact tttgcctcgg ttacaatagc aaatggctga aattatgctt
    12481 acgatatgat ttttaaaagt tgagtctgct ctgaactaag tttttcagat atttatgtat
    12541 ccaacatttg actatatctc ggatgtccct tttctaaata gttgttgatt catcttttta
    12601 gctgttttag ttaagagaca tcaaaaaaga ttacattcga ctttcaagta tagctatcac
    12661 tttttcgggt gtaaccaatt tttgttcacc tatagctaca aatccgtttc ttttcagatt
    12721 ctcaactact tgtacgattg tagttttatt aagcttataa tcggataatc ttgttttagc
    12781 gccaaggctg ataaaaaaag cttcagtttt aaaaatagct ttttcaatac gttcttcatc
    12841 atctccatta tttatgtccc atatcctttc agcatattgc aacagttttt cttttttaat
    12901 ctgcgacatc acgcgcatca ttcccggcca tactatagcc aaagtgcttg catgatcaat
    12961 attatgcaac actgtaagtt catggcctat tttgtgtgta gcccaatcag ttatcaatcc
    13021 tgtagcaatc agtccattaa gcgccattgt tgaacaccac ataatattct tggcaacagt
    13081 catattttca ggttcattta cataattcaa gccttcttca atcagagttt tcattatggc
    13141 ttcggcaaac cggtcattta gtggggcatt ttgttggaaa gtcaaatact gttctgtaac
    13201 atgtacaaat gcatcaataa caccgtttat tcgttggctg tgtggcagag tgcgagtgaa
    13261 attcggatcg agaaccgaaa atcgcggata agtaaatgga ggtgtaccaa aatcaagttt
    13321 ctggccggtt tcctttcgtg atatcaccgc aaaagcattc atttccgatc cggttgccgg
    13381 taaggtcaac acggcgccaa aaggaattac ggatgtaacc tttaattgat tctctaatat
    13441 atcccatggt tcgccatcaa ataaggatgc agcagcaatg aatttggttc catcaagcac
    13501 cgaacctcca cctactgata atagaaaatc aattttctct ttccgacaca aatccacagc
    13561 cttcataaga gtagaatatt gcggatttgg ttcaatacca ccaaactcaa taaaaaagaa
    13621 gtcttcgaga acgctgacta cctgatgata aatcc
//
LOCUS       DAOOAI010000061        13561 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_5855_length_13561_cov_4.758626, whole genome shotgun sequence.
ACCESSION   DAOOAI010000061 DAOOAI010000000
VERSION     DAOOAI010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13561)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13561)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13561
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5855_length_13561_cov_4.758626"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(58..1053)
                     /locus_tag="PK345_05680"
     CDS             complement(58..1053)
                     /locus_tag="PK345_05680"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34541.1"
                     /translation="MIKQKYFSKKTMIFFTSFAVLIFSLVFTHAVGDQSDNFTNPENH
                     NSRQSYSPFIDNGKHWNVMHEGYGEKRTIEYYISTQDTTINDTVYRKVLVNDQYNYYP
                     SGIAGFIREDTAERRVYFRPYYAEWHPIHDGLLYDFSIDVGDTVDVYGLYYCQTYSNA
                     YKVVSISTIELLNGEQRTLWDLHPVEENVQKLDQWIEGVGSINGVLFPGCYEMATISL
                     YLELLCYYEDDVKLYMSARDTCFVNWTSGVQTNMQHDIQPYPNPVSNVLYLATVCESE
                     RFNAYEIYNLSGLKVAEGRFSDHNYTIPVDILRSGMYLLRIKNESNAHNFKFIKN"
     gene            complement(1373..3718)
                     /gene="pbpC"
                     /locus_tag="PK345_05685"
     CDS             complement(1373..3718)
                     /gene="pbpC"
                     /locus_tag="PK345_05685"
                     /inference="COORDINATES: protein motif:HMM:TIGR02073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HPX34542.1"
                     /translation="MRWKPLSSVIIIVLLFLLFWGIYHFLPPYRFTKPVSTVVFDRNG
                     ELLGARIATDGQWRFPVSIAVPEKFKICLIEFEDQYYYRHPGINPLSIVRATYQNLRA
                     GKVVSGGSTITMQLIRLSREARKRDIFQKMIEAVLAVYSEIHFSKDENLRQYVSAAPF
                     GGNVVGLEAAAWRYFGRSANNLSWAESACLAVLPNAPSLIHPGRNRAALKAKRDYLLD
                     RLYKKGKIDKETFELSLLENLPEKPFPLPETATHLVGHLAFTLPENQFFTSIHSSLQN
                     QIFEIVERHHKILESNKIHNIAALVLDTRSGEVLSYIGNTKKSFPGDHGNHVDIIQRP
                     RSSGSLLKPILYAVMLSEGEIMPEMLVPDIPTTISNFSPKNFNNTYDGAVTAREALIR
                     SLNIPAVRMLKDFGVERFYQVLKNAGFSSINRGSENYGLSLILGGAEITLWDVCGIYR
                     AMAFKLLEYDNQLSKQKITLLKPMLLPDGDDSCEIIDLHALDEAAIYLTLDAMREVHR
                     PEAEIGWEWFSNSGAIAWKTGTSFGFRDAWSVGITPDHVVGVWVGNADGEGRPGLTGI
                     NTAAPLMFEIFNILPAKTAWFAKPWDKMESIAVCRQSGMRVSQYCTDIDSIWVNSAAL
                     NSAPCPYHKQIHLDQSEQYKVNAECYPFEKIVTKSWFELPPAMAFYYKARNPFYRQPP
                     PWLPGCYYTDEETMQIVWPDKPTRIFIPREIDSTVGEVVFEVAHSRPDAIVFWYLNDE
                     FIGQTNYFHKKSFRRPTGKYKLLLMDESGNILSHNFEIVNK"
     gene            4078..5706
                     /locus_tag="PK345_05690"
     CDS             4078..5706
                     /locus_tag="PK345_05690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34543.1"
                     /translation="MNRRADISIFTSLLFLILFQGCVAKRLGKQAARFESAGMFAKAA
                     DLYYKASLKKPNVVEFKTGLRRSGQIHLEELSQLVNQAYINRDYHKAVYDFLALKGFL
                     NKVKYAGVELNIDYSTQRIFENARELYLSDRYEYGQKLIGESDFDGAKKVFNEIHQIL
                     PDFRDTRSYLNMATIEPIYLRGVNLFSQRKYMDAYQEWEKVTMYDGGYKDVNSLMHQA
                     LNERYKEGVLFLINENFSAAALALGEVYKKNPKFEDVKNLYLEARNEPIYRSAMVDLN
                     AGKCRAAYYGFEKVIADVGAYKDADAKRKRALDCAQYPIALATGKISDNQKDAIEFEN
                     TLINTLLKKRDPFLKIHKLEIVNSTLMKALKKETLIPDRNQLKELHDKNGIKAVLTLL
                     FSNYRKADGALEDRQKTGFERKKITITTGETIFDDKKVTYYEYQKENQFSIEISYQLI
                     STLTGEILLSDKLNGAETDKIHYAIYDGDTKILYPAVKIDNNYFVDDKNYSSLQALLK
                     SSQQITTPDKQRDKVYGVLAEKISASLSNFNPER"
     gene            5703..7073
                     /locus_tag="PK345_05695"
     CDS             5703..7073
                     /locus_tag="PK345_05695"
                     /inference="COORDINATES: protein motif:HMM:NF014248.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPP20 family lipoprotein"
                     /protein_id="HPX34544.1"
                     /translation="MRVIILYVSLLVIASGCSSSKRAGKTDMLKDAPEWVQQTPNTPS
                     YYHGIGMIYKSGVQNYRERARQVALSELAGNISVNISSSSVLNQFEYDHTYSEFFKDE
                     IRMNTQEQLEGFEFVEQWENDQQYWVYYRLSKSTWEEIKKERRDKALRLSKAQLEQAN
                     SYTAKGNYVDALNFYICAIEEIRDFLGEDLRISFDNEERSYATSLKTAIINDLQRLKI
                     VFPSSAMEIGPDSKNKNLSFEAAIIDNCERPVSGIPIITRFTWLPGYKIETTSDINGK
                     FKIAIGTIDFRKQSEKIISEINLDKIVSDNTNDILVKRLFETMKPHSYILPIKLTAPA
                     FDIVLNEKNLGQTIINSGLREEIVRLLKQNGFELVSANNKGDYSIIVEANTQKGSERN
                     GLFSAKLSAAVLVKDRSNKTVYNRKVEDISGLGSGFSEAGRDAFRSFISKYRISIYPE
                     IVQNLF"
     gene            complement(7446..11435)
                     /locus_tag="PK345_05700"
     CDS             complement(7446..11435)
                     /locus_tag="PK345_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPX34545.1"
                     /translation="MKKNFYGIIIISVFLFLVSGLKSQSAEFMMQGWYWDYPKTVQGA
                     NWADTLRLKAAEIGDAGFTFLWFPPLSRTASGGLSNGYDPKDLFDYGISEQGATGFGT
                     ENDVGNLISALNTNSIKAVADMVYNHRNGGSVQDNPGLKNYIVNFYTNLKADEGANPF
                     PYDRMRVVLPLGGGSGNGAGDYYFKFRSRSQHSKFYNWEYKIYMSTDKVGWQGLPDIY
                     EAEPNGGGDCNQGCNEVYLGRNILGNIDSDACGIDEFKLTLTVDDFNAGGDAIYIYFD
                     GRNSGYSDLYIHGIWSVPRNANIVNDLYYQTYTDFSWAPSKRGKMDWSYFKPNTDRET
                     YLAGDWDNMYFYYDYDQFQNKTRDTLFAWTRWNWSNIGIRGFRLDAVKHFSPEFVGDL
                     LDNLYENGMSPTMVVGEWYSTNVDELAGWVNSVYYHMNEATKEAIKPRIFDFSLREAL
                     RRACDDNNYDVRNVFNAGIVDQNKLYGSNVVTFVNNHDFRDQSGFASLIQNDAILAYA
                     YILTNNQIGLPTVFYPDYFGYPSDAETGYQSYHPTNKGSLKNQINKLMSIHNQYIYGA
                     NSRIYLNNFTGGRPNSLGENNNSALVYQIKGTTGNRDIVVAINFSDSQIGFSQEIDGV
                     AMGTQFEDLTGNAIYKTPEVENANGINNSIWISLPARSYAVYRVGSWGLWNAQRSYAG
                     FNVKGNSVSRTFWDEGTGNIQEYDFGTFIGSDIFQLTSYDIKTWKKSGADVTGGNLFY
                     TIYPKGQRPQSPVFISKTLNWMEDIGGQATGDQKWGFSGQSINLLNGLTSGEYTIEFY
                     AQMDGTNPNKSEYDSNNGLNYKAYFRYNYVRSFADGNWSSSAIWLDQIIPDSSVVSAE
                     INHNINLTEDISVNDLLLFGGSLTVAPAKSLTVFGTSSSALDEAALLLQSNEFGTASL
                     LHNTSGLNATVQRYIPAADWDEPGDGWHLVASPVIGQPIDGVWTPTGQGNDYDFYQWN
                     EPNVTWLNQKENANQIVEFETGRGYFAAYQQLSTKAFAGPLQVTAVTVNLTKTGAGGY
                     YGWNLLGNPFTTALDWSHSSWERNVEIGGIAKIWSNGAYIDIDESEPVIPSANGFFVF
                     TNADDTELTIPAGARIHSNQNWYKLAGNRVLLTARHESSDMVQRTVIKENPNATDGFD
                     LKFDSRFMAGYAPMFYSMADDEKLSTNTLPKLNHQTAIPLQFIANKDATYEIELVETF
                     YEKEAILIDLKLNLEHKFSVNPVYSFSASPYDDPERFILYFGSLGIENQYDNESVWAW
                     YSDDQLFLITDGTWHLVNVFDIQGRSLLTKAIMGESFVALPFRFAPGLYVLQLSSGHM
                     VKPVKLLVR"
     gene            complement(11767..12447)
                     /locus_tag="PK345_05705"
     CDS             complement(11767..12447)
                     /locus_tag="PK345_05705"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPX34546.1"
                     /translation="MISIQNLSKSFGAKQVLKNITIQFNKGQVYGIVGENGAGKTTLF
                     RCIAGLENYEGQIISELSPLKNHLGLLFTEPFFFTKITGREYIRLLCNARKERVSNID
                     DKNIFDLPLNQYASTYSTGMKKKLALLAILLQNNQCFVLDEPFNGVDIHSNIIIIDLI
                     HKLKDLGKTIIISSHIFSTLSDTCDEIYLLKEGEIIQKVFREDFEALELEMREFTVGN
                     KIERLELK"
     gene            complement(12444..13367)
                     /locus_tag="PK345_05710"
     CDS             complement(12444..13367)
                     /locus_tag="PK345_05710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34547.1"
                     /translation="MKEYFKLQFLMTIRKLSDGSRPIVGYLLALLISLVFVGVSFWLL
                     SGKYDNAPYIYVFASLFFISKLSEIRRNDFLKICFANRQYRKVRMLENLLIALPFVLF
                     LVYKQHFYLTFILVAITVLMALLNFKATYNIIIPTPFYKKPFEFTVGFRNSFFMFFIA
                     YVLAIIAVKVDNFNLGIFALVLIFLTIFGYYLKPENEYFIWVYNCTPTKFLIEKIKTA
                     LLFSFCLSLPVLLLLSILYFEHIGVMLIFTFLGFLYLATWILAKYSAYPEEIGIMQGI
                     IFAFGLFPPMLIVIIPIFANQSINKLKEILK"
BASE COUNT         4283 a         2752 c         2485 g         4041 t
ORIGIN      
        1 gtatgcatta atagctaata aaatcaataa tgcagataga atgtttttca taatttatca
       61 atttttaata aacttaaaat tatgtgcatt cgattcattt ttgatccgaa gcaaatacat
      121 tccgcttcga aggatgtcaa ccggtatcgt gtagttatgg tcggaaaatc tgccttcggc
      181 aacttttaat ccgctcagat tgtagatttc ataagcatta aagcgctcac tttcgcaaac
      241 tgttgctaaa tacaaaacgt ttgaaacggg attgggatag ggttgtatat cgtgttgcat
      301 gttggtttgt actcctgatg tccaatttac aaaacaggta tcacgagctg acatgtagag
      361 cttcacatca tcttcgtaat aacaaagtaa ctcaagatac aatgaaatag ttgccatttc
      421 ataacaaccc ggaaacaaca caccattaat acttccaact ccctcgatcc attggtccaa
      481 tttctgtaca ttttcttcta ccggatgaag atcccacaat gtccgctgct cgccgtttaa
      541 taattcaatc gtgctaatgg ataccacttt ataagcgttt gagtaagtct gacaataata
      601 gagtccgtaa acatccaccg tatcgcccac atctatcgaa aaatcataca acaacccgtc
      661 gtgaattgga tgccactcag cataataggg tctgaaatat acacgccgtt cagcggtatc
      721 ttcgcggata aagcctgcaa tgcccgaagg ataataatta tattggtcat ttaccaaaac
      781 ctttctgtac actgtgtcat taattgttgt atcctgtgtt gatatgtagt attcgatagt
      841 tcttttctca ccatacccct cgtgcattac attccaatgt tttccattgt caataaaggg
      901 gctataagat tgtcgtgagt tgtgattttc cggatttgta aaattatcac tttgatcgcc
      961 aacggcatgg gtgaaaacca atgaaaagat caaaactgca aaagaagtaa aaaaaatcat
     1021 tgttttcttt gagaagtatt tttgtttaat cataaaagta aaattttggt tattagttaa
     1081 aagcaataga ttttataaag tttagtctga tacaaagcaa aattacatta aaaaggaaac
     1141 aaatcgctgt aaaattccgg ctaaaaaagt tactggcctg tttcataatc acctcctctt
     1201 aactttttga aattaaaata tcggcatcaa atgtaataaa atattttaaa cgtttcgttt
     1261 tttttgtaat aaacatacaa atatcttagg taagttcgaa aaagtcttat tatttggggt
     1321 tacttttcct aaacctaaag tttctttgac tttaatgtct taacttttaa atttatttgt
     1381 ttactatctc aaagttatgg gaaagtatat ttcctgattc atccattagt aaaagtttat
     1441 acttaccggt tgggcgtctg aaactttttt tatggaaata gttggtttgg cctataaatt
     1501 cgtcattcag ataccagaat actatggcat cgggtcgtga atgtgccact tcgaatacca
     1561 cctcccccac tgtgctatca atctcgcgtg ggatgaaaat acgggttggc ttgtcgggcc
     1621 aaacgatttg catagtctct tcgtcggtat aataacaacc gggcagccat ggcgggggct
     1681 ggcgataaaa cgggtttcgg gctttatagt aaaacgccat tgccggcggc agttcaaacc
     1741 atgatttagt tacaatcttt tcgaacggat aacactcggc atttacctta tattgctctg
     1801 attggtctaa atggatctgt ttatgatagg ggcatggcgc tgagttgagc gctgcgctgt
     1861 taacccaaat gctatcaata tcggtgcagt attgcgaaac cctcattccg ctctgcctgc
     1921 ataccgctat actctccatc ttatcccacg gctttgcgaa ccatgccgtt ttggccggca
     1981 atatgttaaa tatctcaaac attagcggcg ctgccgtgtt gatacctgta agcccgggtc
     2041 ttccttcgcc gtcggcgttt ccaacccata ctccaaccac atgatcgggg gtgataccaa
     2101 ccgaccacgc atctctgaaa ccaaaacttg tccctgtttt ccatgctatc gctcccgaat
     2161 tgctaaacca ctcccaaccg atctccgcct caggacgatg tacctccctc atagcatcaa
     2221 gggtaagata aatagcagct tcatcaagcg catgcaggtc aatgatctcg cagctatcat
     2281 caccgtcggg caacagcatc ggttttaata atgttatctt ctgttttgaa agctgattat
     2341 catattcaag cagtttgaaa gccatagcgc gataaatacc gcaaacatcc cacagcgtaa
     2401 tttcggctcc gccaaggatc aacgacaaac cgtaattttc tgatccgcga ttgatagaag
     2461 aaaagcctgc gtttttcaaa acttggtaaa aacgctctac tccaaagtct ttaagcatcc
     2521 ttactgccgg tatgtttagc gaacggataa gcgcttccct tgccgttaca gcgccatcgt
     2581 atgtgttatt aaaattttta ggcgagaagt tgctgatggt agtggggata tcgggcacga
     2641 gcatttcagg catgatctca ccttcgctga gcataacagc atagagtatc ggctttagta
     2701 aactgccgct actccgcggc cgttggatga tgtcaacatg gttaccatga tccccgggga
     2761 agcttttttt tgtgtttccg atataagaca atacctcacc cgaacgggta tcaagaacta
     2821 atgccgcgat attatgaatc ttgttgcttt ctaaaatctt atgatggcgt tcgacaattt
     2881 cgaagatctg attttgcaaa gatgaatgaa tggaggtaaa aaattgattt tcgggcaagg
     2941 taaacgccaa atgaccgaca agatgtgtag cggtttccgg taacggaaat ggcttttcgg
     3001 gcaagttttc aagtaaagag agttcgaagg tctctttgtc aatttttcct tttttataaa
     3061 gtctatctaa caggtaatcc ctttttgcct tcaacgctgc acgattgcgt cccggatgta
     3121 tcaaagaggg ggcatttggc aaaactgcca gacaggctga ttcggcccat gacagattgt
     3181 tggcgcttcg tccaaaataa cgccatgctg ccgcctcaag gccaaccaca ttgcctccaa
     3241 atggcgccgc tgacacgtat tgacgcaggt tttcatcttt actaaaatga atttcggaat
     3301 aaactgccag aacagcttca atcatttttt gaaagatatc tcttttacgg gcttcacgcg
     3361 aaagccttat gagttgcata gtaatggtag aaccaccact cacaactttt cctgcccgca
     3421 aattttgata agtagcccgc acaatggaga gtggattgat ccccggatgt cggtaatagt
     3481 attgatcctc gaattctatc aagcagattt taaatttctc cggaacagct atggatacag
     3541 gaaaacgcca ttggccgtca gttgctattc ttgctcccaa cagctccccg tttcgatcaa
     3601 aaacaaccgt gcttaccggt ttagtaaaac gataaggggg cagaaaatga tagatacccc
     3661 aaaacagcaa aaagagcaaa acaattatga taacagaact caacggcttc cacctcattt
     3721 tagtaatcag aaaactatct aaggtcttcc atacagagca acacattccc tttatccttg
     3781 atcggaaaac atataatggc ctgttattca taaaaataag tagaaatgtt tattttgaat
     3841 ttactcaatg ctctatcaaa gtcaacttca ctaccgcctt ctgatgtttc atttacaaag
     3901 atagccttat gaaaattgct gttatacttt agttagtcct gcgccaaatt ataaatataa
     3961 aatactgatc ccattattgt tacgatacta tctccatata aaaaattcga ataaagaact
     4021 ttcagtcatc atttgcttaa taaacaaatg ctacctttac accacaattt ttttcttatg
     4081 aatcgcagag ctgatatttc catttttacc tctcttttat ttttaattct ctttcaaggt
     4141 tgtgttgcaa aacgcttggg aaagcaggcg gcgaggtttg aatctgcagg tatgtttgct
     4201 aaggctgctg atctttatta caaagcatcg ctaaaaaagc caaatgtcgt ggaattcaaa
     4261 accggattaa gacgttcagg gcaaatccat ttagaagagc tttcgcaact tgttaatcag
     4321 gcttacataa accgtgatta tcataaggct gtttacgact tccttgcgct gaaaggattt
     4381 cttaataaag taaaatatgc cggcgttgaa ctgaatattg attattctac gcaacggatc
     4441 tttgagaatg caagggagct ttaccttagc gaccgatatg agtatgggca gaaactgatt
     4501 ggagagagcg attttgatgg agccaaaaag gttttcaatg aaattcatca aatactgccc
     4561 gactttagag atacacgtag ctatttaaac atggctacga ttgagccaat ctatttaagg
     4621 ggagttaacc tttttagcca acgcaagtat atggatgctt atcaggaatg ggaaaaggta
     4681 acaatgtacg acggcggcta taaagatgtg aactccctaa tgcatcaggc tttaaatgag
     4741 agatataagg agggagtgtt gtttttaata aatgaaaact ttagcgccgc tgctttagcc
     4801 ttaggtgagg tttataagaa aaatccgaag ttcgaagatg taaaaaatct ttatttagag
     4861 gctcgtaatg agccgatata ccgaagcgcc atggttgacc ttaatgctgg caaatgccgc
     4921 gcagcctact atggtttcga gaaggtgatc gccgatgtgg gagcatataa agatgctgac
     4981 gcaaaacgaa aaagggcgct tgactgtgct caatacccaa tagcgcttgc cacaggaaaa
     5041 atatcggata accagaagga tgcaatagag tttgaaaata cccttattaa cactctccta
     5101 aaaaagaggg atcctttttt gaaaatccat aaacttgaaa ttgtcaatag tactttaatg
     5161 aaggctctta aaaaggagac cttgatacct gacagaaatc agttaaaaga gctacacgat
     5221 aaaaatggaa ttaaagcagt attaacgctt cttttttcaa attacagaaa ggctgatggc
     5281 gcattggaag acagacaaaa aaccggcttt gaacggaaga agataactat aaccaccgga
     5341 gagacaatct ttgatgataa aaaggttact tattatgaat atcagaaaga aaaccaattt
     5401 tcgatagaga tctcttatca gctaatctct acccttacag gggaaatttt attgtcagat
     5461 aagttgaatg gggctgaaac cgacaaaata cattatgcaa tttatgatgg tgatacgaaa
     5521 atcctctatc ctgctgtaaa aattgacaac aactattttg ttgatgataa aaactattcc
     5581 tcgctgcaag cgcttctaaa atcaagtcaa caaataacca cacctgataa gcagcgggat
     5641 aaggtgtacg gtgttcttgc cgaaaaaata tctgcttcat taagtaattt taatcctgag
     5701 agatgagagt aattatcctt tatgtttcac tgttagtaat agcttccggt tgttcttcaa
     5761 gcaaaagagc cggtaagact gatatgctaa aggatgctcc cgagtgggtt caacaaaccc
     5821 caaatacccc atcatactac catggtattg gaatgattta caaatcgggt gtgcaaaatt
     5881 atcgtgagcg ggcgcgtcag gttgcccttt ccgagctggc cggcaatatt tcggtaaata
     5941 tctcttcgtc atcggtatta aaccaatttg aatacgacca tacttacagc gaatttttta
     6001 aagatgaaat tcgtatgaac acgcaagagc agcttgaggg atttgaattt gtcgaacagt
     6061 gggaaaatga tcagcaatat tgggtttatt accggttatc gaaatcaaca tgggaagaga
     6121 taaaaaagga acgaagggat aaggctctta ggttgtcgaa agcccagtta gaacaggcta
     6181 atagctatac tgccaaagga aattatgttg atgctctaaa tttttacatc tgtgctattg
     6241 aagaaatcag agatttctta ggtgaagatc ttagaatatc ctttgataac gaagagcgat
     6301 cctatgcaac atccttgaaa actgcaataa tcaatgattt gcaaagatta aaaatcgttt
     6361 ttccatcatc tgcaatggaa attggccccg attcaaaaaa caaaaaccta tctttcgaag
     6421 cagccataat tgacaactgt gagcgtcctg tgagcggaat accgattata acacgattta
     6481 cctggctgcc gggttataaa attgaaacta catcagatat aaacggaaag tttaaaatcg
     6541 ctattggaac aattgatttt cgaaaacaat ccgaaaaaat tatttcagag atcaaccttg
     6601 acaaaatagt tagcgataat actaatgata tattagtaaa aaggcttttt gagactatga
     6661 aaccccatag ttatatattg ccgataaaat tgacagctcc ggcctttgat attgtgttaa
     6721 acgaaaaaaa cctcgggcaa acaattatca attccgggtt gagagaggag atagttcgtt
     6781 tgctaaaaca gaatggcttt gagttagtat ctgcaaataa caaaggtgat tactcgataa
     6841 ttgttgaagc aaatactcaa aaaggcagcg agcgcaatgg tctcttttct gcaaagctaa
     6901 gcgccgctgt attagtaaaa gacagatcca ataaaacggt ttacaacagg aaggtggagg
     6961 acatctccgg attaggttca ggattttctg aagccggaag ggatgccttt cgctcattta
     7021 taagtaagta tcgaatctct atttatcccg aaatagttca aaatctgttt taactcatat
     7081 tccaactatg gatttatcaa tgccaacaaa aaggagctaa cttaaaagca gcgcttcagt
     7141 tcaagaaaaa ggttttctat tgataaaatt agagaccttt acacgataaa tagttagaat
     7201 ttgataatca attggaaatc agtagaaaca gttattaatc gttactacgc taaggcttat
     7261 aatctttgtg gcgaaaaaaa acctatagcg ggttagccct attcaaaatg ctacttcttt
     7321 cggttgaaaa ctccgtgaaa ctatgtgtaa gaaaaaagta acacagagga acacagagga
     7381 aataaaaact ctgtgaaaca ccgtgaaaaa ctctgaatcc gtctaaacta acagaagcct
     7441 aatgcttatc tcacaagcaa tttaaccggt tttaccatat ggccggaaga gagttgcaac
     7501 acatataacc ctggcgcaaa acggaatggc aaagcaacaa aactctctcc cataatagct
     7561 ttagttaata atgatcttcc ttgaatatca aaaacgttca caagatgcca tgttccatcc
     7621 gtaattaaaa aaagctgatc atcggaatac caagcccaaa cactttcgtt atcatattga
     7681 ttttcgattc ctaacgaacc gaaataaagg ataaacctct ccggatcatc atatggagat
     7741 gcagaaaatg aataaacagg gtttactgaa aatttgtgtt ctaagtttag cttcaggtca
     7801 attagaatag cctctttctc ataaaacgtt tcaactaatt ctatctcgta agttgcatct
     7861 ttattagcta taaattgcaa tgggatagct gtttgatgat tgagcttggg taaggtatta
     7921 gtagataatt tttcgtcatc agccattgaa taaaacattg gtgcataccc agccataaaa
     7981 cgactatcaa atttaagatc aaatccatca gtggcatttg gattctcttt aatcactgtt
     8041 cgttgaacca tatcgctact ttcatgtcgt gcagtaagca aaaccctatt tccagccaat
     8101 ttataccagt tttggttgga atggattctt gctccagcag gaattgtcaa ttcagtatca
     8161 tcagcattag taaagacaaa aaatccattt gcggagggta taaccggctc cgattcgtca
     8221 atatcaatat aagcgccgtt tgaccatatc ttagcaatac caccgatttc cacattccgt
     8281 tcccagctac tgtgagacca atcaagcgct gttgtaaatg ggtttccaag gaggttccag
     8341 ccataatagc caccagcgcc tgtttttgtg agattcacag ttacagcggt tacctgtaaa
     8401 ggtccggcaa aggccttagt acttaactgc tgataggcag caaagtatcc tctgcctgtt
     8461 tcaaactcaa caatctggtt tgcattctct ttctgattaa gccatgttac attaggctcg
     8521 ttccattggt aaaaatcata atcattaccc tgccctgtgg gcgtccatac tccgtcaata
     8581 ggttggccta tgacgggtga ggctacaagg tgccagccat cgccaggctc atcccagtcg
     8641 gcagcgggaa tatagcgctg aacagttgca ttcaaacccg atgtgttatg aagaagtgaa
     8701 gccgtcccaa actcattgga ttgaagcaat agagccgctt catccaaagc gcttgaagat
     8761 gtgccgaaaa ctgtcaggct cttagccgga gctactgtga gactaccgcc aaaaagcaga
     8821 agatcattta cagatatgtc ttctgtaaga ttgatattat ggtttatttc agcgcttaca
     8881 acagagctgt cgggtataat ctgatcgagc cagatagcag atgaactcca gttgccatca
     8941 gcaaaagaac gaacataatt atacctgaaa taagctttat agtttaaacc gttgttactg
     9001 tcgtattctg atttgtttgg gttggtacca tccatttgag cataaaactc aatagtatat
     9061 tcacccgatg ttagtccatt caaaagattt attgactggc cagagaagcc ccatttctga
     9121 tcgcctgtag cctgaccgcc aatatcttcc atccaattta acgtcttact aatgaaaacc
     9181 ggtgattgcg gcctctgacc tttcggataa atagtataaa agaggttccc tccggtaaca
     9241 tcggcgccgc tctttttcca tgtctttata tcataacttg tcaactgaaa aatatctgat
     9301 ccaatgaaag taccgaaatc atattcctga atgttccctg tgccttcatc ccaaaaagta
     9361 cgcgacacgg aatttccctt cacattaaac cctgcatagg agcgctgggc gttccacaga
     9421 ccccaggagc caacacgata aacagcatag cttcgagccg gaagacttat ccaaatcgaa
     9481 ttatttatac cattagcatt ttccacttcg ggagttttat aaattgcgtt acctgttaaa
     9541 tcttcaaact gagtgcccat tgccacgcca tcaatttcct gactaaagcc aatctgcgaa
     9601 tcactgaaat ttattgctac tacaatatct ctatttccag tcgttccttt aatttgatat
     9661 acaagcgctg aattattatt ttctccaaga ctgttaggcc ttccgcctgt aaaattattt
     9721 agatatatcc ggctgttagc tccatatatg tattgattat gaatactcat cagcttgtta
     9781 atctgatttt ttaacgaccc cttatttgtg gggtggtatg actgatatcc agtctctgca
     9841 tcggatggat aaccaaaata atcggggtaa aataccgtag gcaaacctat ctgattattt
     9901 gtcaaaatat atgcataagc aagaatagca tcattttgga taagagaagc aaatcctgat
     9961 tgatcgcgga agtcatggtt atttacaaaa gttactacat ttgagccgta caatttattc
    10021 tgatctacaa ttccggcgtt aaaaacattt cgaacatcat aattattatc atcacaagcc
    10081 cgtcttaacg cttctcttaa tgaaaaatca aagattcttg gctttatagc ctcttttgtt
    10141 gcctcattca tgtgataata gacactattg acccatccgg ccaattcatc aacatttgta
    10201 ctgtaccact cgccaacgac catcgtagga ctcataccgt tttcatacag gttatcaagc
    10261 aaatcgccaa caaattcagg tgaaaaatgc ttaacggcat ctagcctgaa tccacgaatg
    10321 ccaatatttg accagttcca gcgagtccat gcaaaaagag tgtctcgcgt tttattttga
    10381 aactgatcat aatcataata aaaatacata ttatcccaat cgccggcaag ataggtttca
    10441 cgatcggtat taggcttgaa atagctccag tccattttac cccttttact tggcgcccaa
    10501 ctaaaatcgg tatatgtttg ataatacaag tcgttgacta tattggcgtt ccttggcacc
    10561 gaccaaattc catgtatgta aagatcggaa tagccgctat ttctgccatc gaaataaata
    10621 tatatggcat cgccacctgc attgaaatca tctacagtta aagtaagttt aaattcatca
    10681 attccacacg catcactgtc aatattaccc aagatatttc ttcctaagta cacttcgtta
    10741 cacccctggt tgcaatcacc accaccgttt ggctctgctt cataaatatc aggtaatccc
    10801 tgccagccaa ccttatcggt gctcatataa attttatatt cccaattgta aaacttacta
    10861 tgttgcgatc tcgaccggaa tttaaaataa taatcgccgg cgccgttccc cgagccgcca
    10921 ccaagaggca acaccaccct catcctatcg taaggaaagg gattagcgcc ttcatcggct
    10981 tttaggtttg tgtagaagtt tactatgtaa tttttcaatc cggggttatc ctgaacactt
    11041 cctccattcc tatgattata aaccatatcg gctaccgctt tgatactgtt agtattcaaa
    11101 gcgctaatca agttccctac atcattttcg gttccaaatc cggtagcgcc ttgttcagaa
    11161 attccatagt caaaaaggtc tttcggatca tagccattgc tcaagcctcc tgaagcagta
    11221 cggctcaaag gagggaacca aagaaaagtg aagcctgcat cgcctatctc ggcagctttt
    11281 agccttaaag tatcagccca gttagcgccc tgaacggttt ttggataatc ccaataccag
    11341 ccttgcatca taaattcagc cgattgcgat tttaaaccgg agactaaaaa cagaaaaacg
    11401 gatataataa ttatcccgta aaaattcttc ttcataatcg taatattaat gtaaagtgat
    11461 attcttcaaa atactaacaa caatataagc taaaaatttt tgctgaccaa actttttgcc
    11521 caaaaaagct caatggaaaa cctggagaca tcttaattcc ctaattcgcc agacaaactt
    11581 aacatattaa ctttaaacga ttgccatatt tgctaaggta cagcatttga aaaccgtcag
    11641 ccgtgctttt gcaattatgt ttattgagct ttgcgggtta tgagctttaa attttgcgct
    11701 tatgcagcca tttattatag ccaatattaa cggtagtgca tttatttgta tctcagccgt
    11761 atccaatcat tttaattcta atctctcaat tttattgccg actgtaaatt ctctcatttc
    11821 aagttctaac gcttcaaaat cttctctgaa cactttttga attatctctc cctctttcag
    11881 caaatagatt tcatcgcagg tgtcgctcaa ggttgaaaat atgtgtgaag aaatgattat
    11941 cgtttttcct aaatctttca atttatgaat aaggtcaata atgattatgt tgctatgaat
    12001 gtcaacgcca ttaaaaggct cgtcaagaac aaagcactga ttgttttgca ataaaattgc
    12061 taacaatgcc aatttctttt tcattcctgt tgagtatgtc gaagcatact gattcaaggg
    12121 taaatcaaat atattcttat cgtcaatgtt cgacactctc tcttttcgag cattgcatag
    12181 taagcggata tattccctcc ctgtgatttt ggtaaagaaa aatggttcgg taaacagcaa
    12241 tcccaaatgg tttttcaatg ggctgagttc cgaaattatt tgcccctcat aattctccaa
    12301 acccgcaata catctaaaca atgttgtttt tcccgctcca ttttcgccca caattccgta
    12361 aacctgccct ttgttaaatt ggatagtaat attttttaaa acttgttttg ctccaaaact
    12421 tttagacaga ttttgtatgc ttatcatttc aaaatttctt taagtttatt tattgattga
    12481 ttagcaaata ttggaataat cacaataagc attggaggaa acaaaccgaa tgcaaagatt
    12541 attccctgca ttatacctat ttcttcggga tatgcagaat acttagccaa aatccaagtt
    12601 gcgagatata ggaatcctaa gaaagtaaaa atgagcataa cgccaatatg ttcaaaatag
    12661 agaatgctca ataaaagaag tactggaagg ctcaaacaaa aggaaaacaa tagtgcggtt
    12721 tttatttttt caatcaaaaa ttttgtaggc gtacaattat agacccagat aaaatactca
    12781 ttttcaggtt ttagataata cccaaaaatc gtgagaaaaa taagtactaa tgcaaaaatg
    12841 ccaagattga aattatccac ttttacagca ataattgcga gtacgtatgc aatgaaaaac
    12901 ataaaaaaac tgtttctaaa acctaccgta aactcaaatg gttttttgta gaatggtgtc
    12961 ggaattatga tattgtaggt tgctttaaaa ttcaacaatg ccattaaaac agtgattgca
    13021 acgagtatga atgtcaaata gaaatgctgt ttatacacga gaaagagtac aaaaggtaat
    13081 gcgataagaa gattttccaa cattcgcact tttctgtatt gtctgtttgc aaagcaaatt
    13141 ttcagaaagt catttcgcct tatttcgctt aactttgaaa taaaaaacaa cgaggcaaaa
    13201 acataaatat atggcgcatt atcatatttg cctgaaagca accaaaaaga gacgcctaca
    13261 aacactaatg aaataagcaa tgcaagcaaa tatccaacta tcgggcgcga gccgtccgat
    13321 aatttccgta ttgtcattaa aaactgtaac ttgaaatact ctttcatcag tacttttttt
    13381 taaccgcagg catcacaaag aatgcacaaa ggtagcagag ttttttcttt gcgctctttg
    13441 cggttttatt attcgttttt ttaaccgcag aggtcgcaaa ggatacgcag aggacgcaga
    13501 gttttctttg cgttctttgc gtaaaatctt tgcgttcttt gcggtttaac tattgttctt
    13561 t
//
LOCUS       DAOOAI010000062        13253 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_6028_length_13253_cov_6.286255, whole genome shotgun sequence.
ACCESSION   DAOOAI010000062 DAOOAI010000000
VERSION     DAOOAI010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13253)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13253)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13253
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6028_length_13253_cov_6.286255"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="PK345_05715"
     CDS             complement(<1..637)
                     /locus_tag="PK345_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019036757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HPX34548.1"
                     /translation="MKEKLTIDKLKAEAKAFCIAESKNKNKSLFGVTDGKAVGTHIEH
                     KFQEQLNSKYKTTIGSSANGIDLPSDDILTDIKVTSIKQPQSSCSFKDAKQKIFGLGY
                     NLLVFVYDKVDDPKTKTSKLDFISCSFISKERTADYTTTYRLREMVKDKANIEDIVAY
                     LNDKNIPADEITLNRLAKQILKTPPVQGYLTISNALQWRLQYQRVVTLDKAV"
     gene            complement(867..1970)
                     /locus_tag="PK345_05720"
     CDS             complement(867..1970)
                     /locus_tag="PK345_05720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34549.1"
                     /translation="MKSERKTCNKCLLDDSIEGVILDSNGVCNYCNEYKPIVAYGESQ
                     LLKILQKARKKKRIYDALVPLSGGKDSTYVLYLAVKKYNLKVLTYTYDNGFMSDYAKQ
                     NIKSSIAKCKIDHIWVKHNEELIHNLYRTVLKLSGEICGVCGIGIERSMLKISESYKT
                     PLILLGHTASEQNSFTSENIYDQDRLKAILKKDKNISNTMINRFLIYPKLNFISSYLY
                     TKMGRFGKKVNILYYLDNPADKEIGEILKKEMDWIEPSESDYTRHFDCIAEPFTNFIR
                     EKRFGSSRRLPQLSNMIRSGEITKEEAIKIILNDREMMQKGNFDNVMKKLNLTENDIE
                     EIGNIPKNVFGDETSLSNLIFEKVRKVVKKNKH"
     gene            complement(2131..3039)
                     /locus_tag="PK345_05725"
     CDS             complement(2131..3039)
                     /locus_tag="PK345_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34550.1"
                     /translation="MKKVLSFIGLILALNSYSQYYYDYDINFEDSSQLFRVIIDTLKY
                     PENTWKIGKPQKTIFNSAYSPPNAIVTDLINPYPANDTSVFLIKHVAQQGFVYPHTAI
                     LSGYYKIDSDTLNDYGLIEFSPDNGETWIDLINDTIYNQYIMWNTQKPVLTGRTHEWT
                     YFYVNIAGLGYVFTINDYDTLLYRFSFISDQQSEEMEGLIYDNLHFEDWWEGISEKSN
                     NQFKSICYPVPASTFFNIEFENNHYKDFDLVIINKLGQTVFSKHNITENRICIKINDF
                     TNGIYFYKLINNDTKQYSINKFVIKQ"
     gene            complement(3594..3926)
                     /locus_tag="PK345_05730"
     CDS             complement(3594..3926)
                     /locus_tag="PK345_05730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPX34551.1"
                     /translation="MIRLFLLSLCLFLFSSCDCWQCANGIVTDATTNEPLDSVLVTSY
                     TKDELTREFLTDSTGKYNVCTYNTGRCNGYLKVTFSKKGYKSQEHNNPPGELNISLEK
                     EKTFANPQ"
     gene            complement(4174..5454)
                     /locus_tag="PK345_05735"
     CDS             complement(4174..5454)
                     /locus_tag="PK345_05735"
                     /inference="COORDINATES: protein motif:HMM:NF039309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HPX34552.1"
                     /translation="MTKLKKIIFIVALVAIAILIAILFFKPIIKYESDDQQEIVDNEE
                     IVEPQIIFGFNVDTLNVHNNTVKANEFLANILQGYNVDYGTIDKLVRETRDVFDVRKI
                     RKGNNYSVITTKDSIPKAKYFIYEISSIDYVVYSLLDSIVAWRGQKEVERHIETASGI
                     INSSLWNSMVESNTDPNLALSLSDIYAWTIDFFGIQKGDHYKVVYENVYVEGKCIGIG
                     RVLAASFNHYGKDNYAFYFVQDSVGEYFDNLGKSLRRAFLKAPLNYRRISSRFSNSRL
                     HPVLKIRRPHHGVDYAAAAGTPVMAIGDGVVQYARWDNKGGGNTVRIKHNSVYSTAYL
                     HLQKYGPGIKSGVKVKQGQVIGYVGSTGLSTGPHLDFRFYKNNKAVDPLKVESPPTDP
                     VKDSNLEQFNIERDEWLDMLNEIEMNTKEESPVI"
     gene            complement(5543..6136)
                     /locus_tag="PK345_05740"
     CDS             complement(5543..6136)
                     /locus_tag="PK345_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004311458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="indolepyruvate oxidoreductase subunit beta"
                     /protein_id="HPX34553.1"
                     /translation="MKIDIILAGVGGQGILSIAATIGLAAVEAGLYLKQAEVHGMSQR
                     GGDVQSNLRLSDKEIHSDLIPYGEADMIIAVEPMESLRHLPMLSKKGWIITNTTAFVN
                     IPNYPDKEALMDEIGKIPNHIALDADTIAKDVGASKSANMVVLGAASPFINIDYSLLE
                     NGIRRLFISKGEDVVNFNLAAMKAGRNITEEKLKDSR"
     gene            complement(6157..7785)
                     /locus_tag="PK345_05745"
     CDS             complement(6157..7785)
                     /locus_tag="PK345_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013816399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPX34554.1"
                     /translation="MHKLLLLGDEAIAQAALDSGISGIFAYPGTPSTEIIEYVMRSKQ
                     SRSGAIKATWATNEKTAMETALGVSYVGKRAMVCMKHVGLNVAADAFVNSAVTGANGG
                     LIVVSADDPSMHSSQNEQDSRFYGKFAMIPVLEPSNQQEAYDMVHYGFDISEKFGIPV
                     LLRITTRLAHSRAGVLRKPERKQNEIRLPSDLKQFILLPALARKRYKGLLASQALFED
                     EAEKSGYNQYIDADDKSLGIIASGIAFNYLKENYPDGNVPYPVLKITQYPVPVRMIAK
                     LERECKRILVLEEGAPVIEEALKGLLSKNLNISGRLDGAIPRDGELNPNIVALALGKP
                     DTRIFDMPAIVVGRPPSLCTGCPHIDTYNALNTALSNFSKGRVFSDIGCYTLGALPPY
                     NAINTCVDMGASITMAKGAANVGFTPTVAVIGDSTFTHSGITGLLDAVNDNSPITIII
                     LDNHTTAMTGGQKSAATGDLLVNICRGVGVPEEHIKVIKPLRKNHEENVKIILGELNH
                     PGVSVIIPQRECIQTLNKRMRAKFAEKEKSKKEV"
     gene            complement(8042..>8236)
                     /locus_tag="PK345_05750"
     CDS             complement(8042..>8236)
                     /locus_tag="PK345_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34555.1"
                     /translation="VDSVTATNYPNHNLFLRKGLILIENLTNLQELINKTFDFATFPC
                     NIKNGDGCPVRAVGILSEGG"
     assembly_gap    8237..8246
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8247..8702)
                     /locus_tag="PK345_05755"
     CDS             complement(<8247..8702)
                     /locus_tag="PK345_05755"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HPX34556.1"
                     /translation="MNIIDLSHPMENGMPTYPGDEPASFELLNSHEKDGYQVMKIKAI
                     THSGTHLDAPAHLIADGATIDKMPVLKFYGKGVVLDCSAVEQNSEIGLKYFIQNEKHI
                     ANADFVLVYTGWDKYWGNNKYYADYPVISTNSAIYLLKFNLKGIGLDTPS"
     gene            9487..9789
                     /locus_tag="PK345_05760"
     CDS             9487..9789
                     /locus_tag="PK345_05760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34557.1"
                     /translation="MQNCWIFTYCVHEWTHYLKNKLLKNWFASVRQPNAFHLCLPPPD
                     AKYRCRYFRFQVSGNPGQAFYLSPGATKIIHETTPSQVIYGFNFNGLPTDFIYNCY"
     gene            complement(10249..10956)
                     /locus_tag="PK345_05765"
     CDS             complement(10249..10956)
                     /locus_tag="PK345_05765"
                     /inference="COORDINATES: protein motif:HMM:NF016176.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HPX34558.1"
                     /translation="MNIFWKTIAFAIIIALASCVSGGKKTETKDAEEVGSIKYDQIHE
                     INTDLSIITWEGFKPTGTHVGTIAFKNGELMFWEGKLIGGSFTIDMLSIVNDDLTDPE
                     MNAKLVNHLLSSDFFEAETYPEAMFEITKVVEIEPTQVDLSKEKGDIIPTHAISGNLT
                     LKDVTKNLTFHANLSLNGSDFSAQTNQFFIDRVDWNVRYGSKKLFAELKDNFINDEMG
                     ISINLVSKEVDNETESN"
     gene            11323..12711
                     /locus_tag="PK345_05770"
     CDS             11323..12711
                     /locus_tag="PK345_05770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018694679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HPX34559.1"
                     /translation="MELPFAESYKIKTVETIRKSSRKEREEWVAAARYNLFNLKSDQV
                     FIDLLTDSGTGAMSNRQWSEMMLGDESYAGASSYYKMKNAIREIIGFEYFLPTHQGRA
                     AENVLFSSLIKEGDIVPGNSHFDTTKGHIEYRKAIPIDCTIDEAFDTTALHPFKGNID
                     LEKLESVLKKYPRERIPLIVVTVTCNSSGGQPVSMQNLKEVYSLAKRYGILVFFDSAR
                     FAENAYFIKLREEGYADKSIREIVKEMFSYADGMTMSSKKDAIVNMGGFIGFRDEQLW
                     KKAQTFNIMFEGFITYGGMSGRDMNALAQGLYEGTEFDYLDTRIRQVAYLGQRITDFG
                     ITVQKPYGGHAIFVDAKRFLPQIPKEEFPAQTLAVELYLEGGVRGVEIGALLADRDPV
                     TRENRYPELELLRLTIPRRVYTNNHMNYVAVALANVWEKRDKIKSGMKIISEAPIMRH
                     FTVELTRVNGLK"
     gene            12773..13069
                     /locus_tag="PK345_05775"
     CDS             12773..13069
                     /locus_tag="PK345_05775"
                     /inference="COORDINATES: protein motif:HMM:NF017416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPX34560.1"
                     /translation="MCGVPYYKVLSDFSNESIVDQWIQNPYYQYFCGEAEFQLPFPCV
                     QIDFVHFRKRIGEEGVELIFKMSIDVQRVRANMKSESDGPNAEAELPSSRLSDM"
     gene            13030..13173
                     /locus_tag="PK345_05780"
                     /pseudo
     CDS             13030..13173
                     /locus_tag="PK345_05780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611528.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
BASE COUNT         4066 a         2513 c         2248 g         4416 t
ORIGIN      
        1 gtactgcctt atcaagcgta acaacccttt ggtattgaag tcgccattgt aaagcatttg
       61 aaatcgtcaa atatccttga acgggtggtg ttttcagaat ttgttttgct aaccgattta
      121 gagtgatttc atcggctggg atatttttat cgttcaggta tgccacaata tcttcaatat
      181 tagccttatc tttaaccatt tcacgtaaac gataggttgt agtataatcg gccgttcgct
      241 cttttgaaat aaatgagcaa ctaataaaat caagcttaga tgttttggtt ttggggtcat
      301 ctaccttgtc ataaacaaaa acaagtaagt tgtatccaag tccaaaaatc ttttgtttag
      361 catctttaaa cgaacaagat gattgcggtt gctttattga agtaacctta atgtctgtaa
      421 gtatgtcgtc agatggtaaa tcaattccat ttgcagatga accaattgtt gttttgtatt
      481 ttgagtttaa ttgctcttga aatttatgtt caatatgtgt tccgactgct tttccgtcag
      541 ttactccaaa aagtgatttg tttttatttt ttgattcagc tatgcaaaag gctttggctt
      601 cagcttttag tttgtctatt gtcagttttt ctttcattct aattccttgt attttagttt
      661 acaaatttaa tcatttttct tgtcatttga tacgctgtcg gttttcttag catgaggcac
      721 aacgtataat ttacgaaacg aagatacaat attagagaaa aaaacaaaaa agagaataat
      781 ggaaagaaaa attttgcaaa gctcccgact tatataccct caccaaaagt gagttgttaa
      841 agattgacta attatagttg attagcttaa tgtttattct tttttaccac ttttctaact
      901 ttttcaaaaa tcaaatttga taatgatgtc tcgtcgccaa aaacattttt ggggatattt
      961 ccgatctctt caatgtcatt ctctgtgaga tttagttttt tcataacatt atcaaaatta
     1021 cctttttgca tcatttctct atcatttaaa ataattttaa tggcttcttc tttggtaatt
     1081 tcaccgcttc ttatcatgtt acttaattgt ggcaaccgtc ttgaactgcc aaaccttttt
     1141 tcccgaataa aattggtaaa cggttctgca atacaatcaa aatgtcttgt ataatcggat
     1201 tcagaaggct ctatccaatc catctctttt tttaagattt ctccaatttc cttgtctgca
     1261 ggattatcca aataatacaa aatgttaact tttttcccaa aacgccccat cttagtataa
     1321 aggtagctac tgataaaatt caattttgga taaatcagaa aacggttaat cattgtgttg
     1381 ctgatattct tatctttttt aagaatagct tttaatctat cctgatcgta gatgttttct
     1441 gatgtaaatg aattttgttc tgaagcagtg tgccctaata gaatcaaagg ggttttgtat
     1501 gattcagaaa ttttaagcat acttctttct atgccaatac cacaaacacc gcatatttca
     1561 cctgaaagtt taagaacagt tcgatagaga ttatgaatta attcttcgtt gtgttttacc
     1621 caaatatggt caatcttaca ttttgcaatc gaagatttaa tattttgttt tgcataatca
     1681 ctcatgaagc cgttatcata agtgtaagtt aaaaccttaa gattgtattt ttttacagct
     1741 aaatataaaa cgtaagtact atctttccct ccactcaacg ggacaagagc atcataaatc
     1801 cttttctttt ttctagcttt ttgtaatatc ttcaacaatt gcgactcgcc gtatgcaact
     1861 attggtttgt attcattaca ataattacag actccattac tgtctaagat aactccctca
     1921 atgctatcat ctaataaaca tttgttacaa gtttttcttt ctgatttcat tttctgtctt
     1981 tttgcataaa cgctaaaggt ttgctcatat agcccgtagc cgtttcttta ctctgcagtt
     2041 catcaatgtt cgatattctc gaatttacaa ttctttttct ttatcaccca aaagccgaga
     2101 ctatgggtta tgacgagctg ttaacaaccg ctattgttta atcacgaatt tattaatcga
     2161 atattgtttt gtgtcgttgt tgattaattt gtagaaatag attccattcg taaaatcatt
     2221 tatttttatg caaattctat tttcagtgat attgtgtttt gaaaatactg tttgtcctaa
     2281 tttattaata atcactaagt cgaaatcttt ataatgatta ttttcaaatt caatgttaaa
     2341 aaaggttgaa gcaggtactg gataacagat tgatttaaat tgattatttg acttttcgct
     2401 tattccctcc caccaatctt caaaatgtag attgtcataa attaatcctt ccatttcttc
     2461 tgattgttga tcacttataa atgaaaaccg gtaaagcaat gtatcatagt catttatagt
     2521 aaaaacatat cccaggccag caatatttac ataaaaataa gtccattcat gagttctacc
     2581 agtaagtaca ggcttctgtg tgttccacat aatatattga ttgtaaatcg tatcgttgat
     2641 aagatctatc caggtttcac cattatcagg agaaaattca attaatccat aatcatttaa
     2701 ggtgtcagaa tcaattttat aataccctga aagaattgcc gtatgtggat atacaaatcc
     2761 ctgctgagcc acatgtttta tcaggaaaac tgatgtatca ttggcagggt atggatttat
     2821 taaatcagta acgatagcat ttggaggtga ataggcagaa ttaaaaatcg tcttttgagg
     2881 tttaccaatt ttccatgtat tttctggata tttaagagtg tctattataa ccctaaacaa
     2941 ttgactacta tcttcaaaat taatatcgta gtcatagtag tattgtgaat aggaattgag
     3001 agctaagatt aatccaataa aacttaatac ttttttcatg ttcttttttt ttaatggtta
     3061 ttaatatcta atttacaaaa cgaagataca acattttaga aaaaaccaaa aaaaaagaga
     3121 ataattaaaa aaaaattgaa gtaaaatctt gcaaagctct cgacttatat accctcacca
     3181 aaagcgagtt gttaaggatt gactaattaa atttcttttc attatgtgtt tttattaaag
     3241 ttcaacttta gtgctgtctt ttagtttaaa agtctgtgct gctatttttg ctatcggttg
     3301 gcggtaaaaa aatgtggggg agtgcggcgt gaggagctag ggctgtctca aaacaaatat
     3361 ttaactaaaa aacagcaagg cgtcttagaa acgccgaaat gcaagacgct gagattgagt
     3421 gcaaaggagc gtacttgaag tacgtaactg agccagtact tcgacaagtt cagcaaccaa
     3481 cgaaagtaac atggttgctg ggcgaagtcg aagcacagca gtagggtact tgtgaaacca
     3541 ccgccagcga cgggcaatcc agcttcccca gctcctttct acaactatat tttttactgc
     3601 gggtttgcaa aagttttttc tttttccaat gaaatattaa gttcgcctgg tggattgtta
     3661 tgttcttgac ttttatatcc ttttttggaa aaagttactt tcaaatatcc attacatcta
     3721 ccagtattat atgtacatac attatatttt cctgttgaat ccgtaagaaa ttccctagtt
     3781 aattcatcct tagtatagga agttaccaaa acactatcaa gaggctcgtt agttgtggca
     3841 tcagtaacaa ttccattggc acactgccaa caatcacaac ttgaaaacag aaataaacat
     3901 aatgagagca gaaataatcg tatcactttt tattatttag aattcaactt ttttgtaatg
     3961 aataattaaa ctcgaattat tatcaatctg gttaaaatgt ttgccagcat ctaatttaca
     4021 aaacaaagct acaaaattcg agcaaaaggc aaaaaaagag aataatggaa agaaaatttt
     4081 tgcaaaactc tcgacttata taccctcacc gaaaataagt tgtaaatgat tgactattta
     4141 ggatttagtg ttcgtcaaaa acttaatccg ggattaaata accggactct cttcttttgt
     4201 gttcatttcg atttcattaa gcatgtccag ccattcatct ctttcgatat taaactgttc
     4261 caagttggaa tcttttactg gatcagtagg tggcgattcc actttaagag gatcaactgc
     4321 cttgttgttt ttataaaacc taaagtcgag atgaggccct gtagaaaggc ctgtgcttcc
     4381 aacatatcct attacctgtc cctgctttac tttaacgcca ctttttatcc ccggtccata
     4441 cttttgtaaa tgcaagtaag ctgttgaata gacggaatta tgctttattc gtactgtatt
     4501 gccaccccct ttattatccc atcgagcata ctgaactaca ccatcgccaa tagccatcac
     4561 aggagttcct gcagctgcag cataatcaac gccatggtga ggacgtctta ttttcaaaac
     4621 cggatgcaag cggctatttg aaaaacggga actgatacga cgataattta acggcgcctt
     4681 caaaaaggca cgcctaagac ttttgccaag gttatcaaaa tactccccta cactgtcctg
     4741 tacaaaataa aaagcataat tatctttacc ataatgatta aaactggcag ctaacactcg
     4801 tccaatccct atacatttac cctctacata cacattttca tacaccacct tataatgatc
     4861 gcctttttga atcccaaaaa aatcaatcgt ccaggcgtaa atatccgaaa gtgagagcgc
     4921 aaggttagga tcggtattgc tttcaaccat cgaattccat aacgagctat taattattcc
     4981 acttgccgtt tcaatatgtc tttcaacctc tttctgccca cgccatgcta caatggaatc
     5041 cagtaagcta tatactacat aatcaattga agatatttcg tagataaaat atttcgcttt
     5101 tggtattgaa tctttggtag ttataaccga gtagttgttt cctttccgta tttttcttac
     5161 gtcaaagaca tcgcgtgtct ctctgaccaa cttgtcaatt gtaccataat caacattata
     5221 tccttgaagt atgttagcta aaaactcatt tgctttaacg gtattattat gcacgttgag
     5281 cgtatcaaca ttaaaaccaa atattatttg cggttcaaca atttcttcat tgtcaacgat
     5341 ttcttgctga tcatcagatt cgtattttat tattggtttg aagaaaagta tagcgatcaa
     5401 aatcgctatt gccactaaag caactataaa aatgattttt tttagtttag tcatatttca
     5461 gggttttacc caaaaactga aataaaaagt gttatcctcc caaagttcta aattttggga
     5521 caaatgtgat tgattattca gattaacggg aatctttcag tttttcttca gtaatatttc
     5581 ttccggcttt catagcagcc aggttgaaat tcacaacatc ttcaccttta ctaataaaca
     5641 aacgccggat accattttcg agcagcgaat aatctatgtt tataaatggt gatgcagcgc
     5701 ccagcactac catattagcc gattttgaag cgcccacatc tttagctatt gtatcggcat
     5761 caagagcaat atggtttgga atttttccta tttcatccat tagcgcctct ttatccgggt
     5821 agtttggtat gtttacaaat gctgttgtat tagtaattat ccaacccttt tttgatagca
     5881 taggcaaatg tcttaacgat tccataggtt caacggcaat gatcatatct gcctcgccgt
     5941 aaggtataag gtcggagtgt atctctttgt cggaaagcct taggtttgat tgaacgtcac
     6001 cgcctcgttg gctcatccca tgaacttcgg cttgtttcag gtaaaggccg gcttcaacag
     6061 ctgccaagcc tatggttgcg gcaatagata atatcccctg gccgcctact cccgctaaaa
     6121 ttatgtctat tttcatgatt taaaattcta tcttaattat acctcttttt tcgatttttc
     6181 tttttctgca aacttagcac gcatacgttt gttaagcgtc tggatacact cgcgttgcgg
     6241 aatgatgacc gacactccgg ggtgattcaa ctctccaagg attatcttta cattctcctc
     6301 atgattttta cgaagaggtt ttattacctt aatatgctcc tccggcaccc ctacaccgcg
     6361 gcagatgttt actaataaat cgcctgttgc tgctgacttt tggcctccgg tcattgcagt
     6421 agtatgatta tctaaaatga tgatcgtaat aggtgaatta tcgtttacag catctaacaa
     6481 acccgttatg ccggagtgcg taaaagttga atccccaata acggcaacgg ttggtgtaaa
     6541 tcctacgttg gctgctcctt tagccattgt aatagaagct cccatgtcaa cacaggtgtt
     6601 aatagcatta taaggcggta aagcgccaag ggtataacag ccaatatcag aaaagacacg
     6661 ccctttagaa aagttgctta acgcagtatt taaagcgttg tatgtgtcaa tgtgagggca
     6721 tccggtacat aatgaaggcg gacggccaac cacgatagcc ggcatatcaa aaatacgggt
     6781 atcgggtttc cctaatgcta aagctacaat atttggattt aattccccat cacgtggaat
     6841 agcgccatca agcctgccgg agatattcaa atttttagat agcaaccctt ttaacgcctc
     6901 ttcaataacc ggagcgccct cttccaacac aaggatacgt ttacattccc tttccagttt
     6961 ggcaatcatt cttacaggaa ctggatattg agttattttt aaaaccggat acggaacatt
     7021 gccgtcagga taattctcct tcaaataatt aaaggcaata ccactggcaa taattcctaa
     7081 agacttgtca tcagcgtcaa tatattgatt gtaacctgat ttttcggctt catcttcaaa
     7141 aagagcctga ctcgccaaca gacctttgta ccttttgcgt gctaatgccg gtagcaatat
     7201 aaattgttta agatcggacg gtaaccgaat ctcattttgt ttgcgttcgg gctttcgtaa
     7261 tacccctgcc cttgaatgag ccaaacgggt tgtgatacga agcaatacgg gaatgccaaa
     7321 tttctcagaa atatcgaaac cgtaatgaac catgtcgtaa gcttcctgct gattgcttgg
     7381 ctcaagaacc gggatcatgg caaattttcc ataaaaacga gaatcctgtt cattttgaga
     7441 tgaatgcatc gaagggtcat cagcagaaac tacgataagg cctccatttg cccctgttac
     7501 agctgaattt acaaaggcat cggcagcaac atttaatccg acatgtttca tgcagaccat
     7561 agctctctta cctacatagg aaaccccaag cgctgtctcc attgcagtct tttcattggt
     7621 agcccatgta gctttgatgg ccccggatcg agattgtttt gaacgcataa cgtattcgat
     7681 gatttcggta gaaggcgtcc caggataggc aaaaattcct gagatcccgg aatctaaagc
     7741 cgcctgcgca atggcttcat cacctaataa aagtaattta tgcataaagt caattttagc
     7801 taataaaaca ataaatagaa tttctacgct acaaaactac atttaaaatt attcttaaaa
     7861 accaaaacgt cctgaaactt tgaatttgtt agatcactaa aaactaaaag gataaaatat
     7921 gttttggaat ttattacatt caaagtgcag cattggaaaa ctgaattaaa cacttcataa
     7981 catcttagta attaatatta tacagaaaat tcttttaact aaaaaatgaa aaaaattact
     8041 gctatccacc ttctgataaa attccaacag ctcttaccgg acagccgtcg ccatttttta
     8101 tattgcaagg aaatgtcgca aaatcaaatg ttttgttaat caactcctgc agattagtta
     8161 aattttcaat caggattagt ccttttctta gaaaaaggtt atgattaggg taattggttg
     8221 ctgttacgga atcaacnnnn nnnnnnggaa ggtgtatcca acccaatccc cttcagattg
     8281 aattttaaaa gataaatggc gctattggtg gatatcacag ggtaatcggc ataatacttg
     8341 ttattacccc aatacttatc ccatcctgta taaacaagga cgaagtcggc atttgcaatg
     8401 tgtttttcgt tttgaataaa atattttaga ccgatttcac tattctgttc cactgcagag
     8461 caatctaaaa ctaccccctt gccatagaat tttaaaacag gcattttatc tattgtcgca
     8521 ccgtcggcaa ttaaatgtgc aggtgcgtca agatgagtac ctgaatgagt tattgctttt
     8581 attttcatta cctggtaccc atctttttca tgtgaattta acagctcaaa tgaggctggt
     8641 tcatcgccag gataggtagg cataccattt tccattggat gcgaaagatc aataatgttc
     8701 atgattcttc aaaacgtaga aaaaatttat taaaggtata ggctgatact ttatttgaat
     8761 tcgacataaa gtatgtaatg attgacaata tttttcttta aaaaaagttg atcatcttag
     8821 cgctatgatg ctatgtttca agttttaaac ttcttttaat gcgataaaat tacagcttta
     8881 tccaattctg caaagatgat ttgagcaaat gaatccgaat taatcaacag aaagcctttt
     8941 taaactgaaa gattttttgt gatggcgctt ctgctttaga gtttgttcgc tattattttg
     9001 attatgttta ttacaaccac ttaatcgttt acgggttggc aaatgcatat tcctatctaa
     9061 ctactaaaaa gttagttctc tttttgtttg cgctaataaa gttctatttg ggataaaaaa
     9121 caaagcggga acgacttctt tcgatttcgt tcccgctcac ttggcatcca ccgtggtgaa
     9181 tgttacgcgc caggttactg ttattctggc cgacctttgc atagcgcctt ttcaggctcc
     9241 tgctccggta gttgttcaat attgttaatc gtttccggtt aacttttttt gaactactgc
     9301 ttttcatgca agcttcatta ctttaatgac tccgtagagt ctcatgtaat gttgcccgac
     9361 accttctttg ttgatacgca tttgtgtcaa cagatccggc atcctggttc ttgcagccct
     9421 gatttccttc cgattggctc tcaggagagc tttcctttag gttatcaggc tttcagataa
     9481 agctttttgc aaaactgttg gattttcacc tactgtgtcc acgaatggac tcactatctg
     9541 aaaaacaagc ttttaaagaa ctggtttgct tcggttaggc aacctaatgc ttttcacctt
     9601 tgcttacctc cacctgatgc taaatatcgt tgccgttatt ttcgctttca ggtttccggc
     9661 aatcccggcc aagcctttta tctctcgcct ggtgcaacaa agataataca cgagaccacc
     9721 ccaagtcaag taatttatgg ttttaatttc aacggcttac caacagattt tatctacaat
     9781 tgttattaac ccacaaaact gtttagtgat aagacttata catgaaaatt ttaaatattt
     9841 acaagttcct atgttgataa ccattaaaca cctccttcaa aataatgata ttcaatatga
     9901 tataaatttt ttaactgctg aatttgctga taaacagaca acttattaac attttgcctg
     9961 attttaacgt caataatctt tatgtttttt ggagtatgat ctttagaaat aaactcaaaa
    10021 aacttgtttt tatagccatt aaattctaaa aataataccc ttaaaatgtc agtaagcaat
    10081 tctgcctggc gtacgaaaaa attctctgtt ttagtacaaa tcttaaactt tctgattatg
    10141 ctataccgat agatggtagg taatatttca attctaacac accaaacagg ctaattacac
    10201 ttcttttaat tcctgtatta acataaaacc aacactttaa aattgctttt aattactttc
    10261 agtctcatta tcaacctctt tagatactag attgatgcta attcccattt cgtcgttgat
    10321 gaaattatcc ttcaactcgg caaataactt tttagaacca taacgaacgt tccaatcaac
    10381 acgatcaatg aaaaactgat ttgtttgagc tgaaaaatcc gaaccattaa gtgataaatt
    10441 tgcatgaaag gttaagtttt tagtaacgtc tttcaaagtt aaattacctg atatagcatg
    10501 tgtaggaatt atgtcgcctt tttctttaga aagatcaacc tgagtgggct caatttcaac
    10561 aacttttgta atttcaaaca tggcttctgg ataggtctct gcctcaaaaa aatcagatga
    10621 taaaaggtga tttacaagct ttgcattcat ctcaggatca gtcaaatcat cattaactat
    10681 cgaaagcatg tcaatggtaa aagaaccacc tatcaatttg ccttcccaaa acattaattc
    10741 accgttttta aaagcaattg tgccgacatg agttccggta ggtttaaatc cttcccaagt
    10801 gataatactt agatcagtgt ttatttcatg gatttggtca tactttattg accccacctc
    10861 ctcggcatct tttgtttctg ttttcttgcc tcccgaaaca caggatgcca aagcaataat
    10921 tattgcaaag gcaatagttt tccaaaaaat attcatactt ctatttgttt aatttaatta
    10981 tagttcagca ttaacaacac accaaataat ttgtttgacc tttttctaaa tttattaaat
    11041 ccgagtgtca gagaacgttt tattgtaata tggtaaaatg cttaatctga atttaaaaca
    11101 aacaccttct tttcgttaaa agtaaaactt tatctttgcc ctattaaaaa ttttaaatgg
    11161 atctttttaa cttattatcg gcacttagta atcgtaagta tctcaatatt aagcgtagag
    11221 acaccttcct ttacatatcc ctctgagttt aacccttagt actctgttat taatgaattc
    11281 ctttcttttc tttttattat taacctttaa gtatttaata tgatagaatt accttttgcg
    11341 gaatcttaca aaattaaaac cgtagaaact attaggaaga gcagtcgtaa agagcgtgaa
    11401 gaatgggttg ctgctgcgcg atataatctt tttaatctga agagtgatca agtttttatt
    11461 gatttactca ccgattcagg cactggtgca atgagtaaca ggcagtggtc ggaaatgatg
    11521 cttggcgacg aaagctatgc tggcgcatcc tcctattaca aaatgaaaaa tgctattcgc
    11581 gaaataattg gctttgaata tttcctgcca acacatcagg ggcgtgctgc cgaaaatgta
    11641 ctcttctcct ctttaattaa agagggagat atcgtccccg gaaattcaca tttcgacacc
    11701 actaaaggac acattgaata tcgaaaagcc atacctattg attgtactat tgacgaagcc
    11761 tttgatacaa ctgctttaca tcctttcaaa ggcaacattg atctggaaaa gcttgaaagc
    11821 gtattaaaaa agtatcctcg cgagcgtatt ccattaatcg ttgttactgt tacctgcaac
    11881 agctccggtg gacaacctgt atcaatgcaa aatttaaaag aggtctattc tttagcgaag
    11941 cgatatggga ttttggtatt tttcgattca gctcgttttg ccgagaatgc ttacttcatt
    12001 aagttacgcg aggagggtta tgctgataag tcaatccgtg aaattgttaa agagatgttt
    12061 agttatgccg atgggatgac aatgagcagc aagaaggatg cgattgtaaa catgggtgga
    12121 tttattggtt tcagagatga gcaattgtgg aaaaaagcac agacttttaa tataatgttc
    12181 gagggattta tcacttatgg cggtatgtcg ggacgcgata tgaatgcact tgcacaagga
    12241 ttatatgagg gaacggaatt cgattacctt gacacacgta tcaggcaagt tgcttattta
    12301 ggccaaagga tcaccgactt tggtatcaca gtacaaaagc cttatggtgg tcatgccatt
    12361 tttgtggatg caaagcgctt tttgccgcaa atcccaaaag aggagttccc cgcacagacc
    12421 ttggccgttg aattgtatct tgaaggcggc gtgcgtggag tagaaattgg cgcgctatta
    12481 gccgaccgtg atcccgtaac acgggaaaac cgatatccgg aactcgaatt actaaggctt
    12541 acaataccac ggcgagtata tactaataat cacatgaatt atgtggctgt cgcactggct
    12601 aatgtttggg aaaaacgcga caaaataaaa tcgggtatga aaattattag cgaagcgcct
    12661 atcatgcgcc atttcactgt cgagttaacg agagtaaatg gtctcaaata gccaatcctt
    12721 aaaaacatat ttaagtcctg tttatcaaca aaatactgtt gatacataaa ttatttgtgg
    12781 tgttccatat tacaaagttt tgagtgattt tagcaacgaa agcattgtag atcaatggat
    12841 tcaaaaccct tattatcaat atttctgtgg agaagcagag tttcagttgc cttttccttg
    12901 cgttcagatt gattttgtac actttcgcaa aagaatcggt gaagaaggtg ttgagttgat
    12961 tttcaaaatg tcaattgatg ttcagcgggt aagagcaaat atgaaaagcg aaagcgacgg
    13021 gccgaatgcc gaagcagagc tgccatcaag ccgattatcg gacatgtaaa acatgattgc
    13081 cgaatgggaa gaaactatat caaaggccaa attggagaca aaatcaatgc attgatggcc
    13141 gcagccgggt tcaatttccg cagattactc cgcaaaatga aggcggaagt tatttttttc
    13201 ttttttcaga ttcaaaaaat tctcaacccc aaaccttctt tggctcctaa act
//
LOCUS       DAOOAI010000063        13112 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_6112_length_13112_cov_4.893391, whole genome shotgun sequence.
ACCESSION   DAOOAI010000063 DAOOAI010000000
VERSION     DAOOAI010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 13112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13112
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6112_length_13112_cov_4.893391"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..396
                     /locus_tag="PK345_05785"
     CDS             <1..396
                     /locus_tag="PK345_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006254915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HPX34561.1"
                     /translation="SIFNMTKAVQRVMLKQRNGSIINMSSVVGLSGNAGQSNYSASKA
                     GLIGFTKSIAQELGSRGVRCNAIAPGFIETAMTNKLSDEVRENWIKGIPLRRSGKPDD
                     VANIAIFLASELSSYVTGQVISVDGGMHM"
     gene            430..2511
                     /locus_tag="PK345_05790"
     CDS             430..2511
                     /locus_tag="PK345_05790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34562.1"
                     /translation="MNIATESPAWLIIFCLLAGLAYSALLYYKNKKEEFNALTLGILS
                     AFRFIAVAVIAFLLLSPMLKSHFRNFEKPILILAQDNSESLIIGKDSAFYRNDYPKEF
                     LKLVDELQKEYTVRTYNFGHKVSEELTFDYTDQQTDISELFEEINTRYSNRNVGGMLL
                     ATDGLYNRGINPVYSAEKLKFPIFAVALGDTSVRKDIFIRKINYNRIAFKGNEFPIEA
                     IVGANLCNEENSILTVSHGGAILFSKNINFNSNHFNETVHITLNAEKAGLQRYRISVR
                     PIEGEISLQNNSQDIFIEVLDTKQKIIILSQSPHPDIAALKEAIESNWAYEVTHSLSN
                     QFSGNIGEYNLIILHQLPGGRDDISRIIAEANKQAVPLLFILGSQSNLVQFNKSGAGI
                     EIQAQKELFNESLPSLNKEFSLFTISDDVLKAIEMFPPLIAPFGEIKTQVSANTLFNQ
                     QIGSLVTGYPLIVFNQTLDNKTGVIAGEGIWRWRLINFQKYGNHNAFNELLSKIIQYL
                     AVKADKSFFRVTGQNIFMENEAILLDAEVYNQSYELINTPDVEITLKNSVGDTYNFLF
                     GKTSNAYQLNAGILPIEDYTYEATVRVGEKLYRSQGEFTVSPVNIEAINTVADHNLLY
                     RLTSKYQGELIYPEQLSTFTKMIKARDDVRTVVYTEKKFSGLVNIFGIFIFIVALLTA
                     EWFIRKRSGSY"
     gene            2665..3897
                     /locus_tag="PK345_05795"
     CDS             2665..3897
                     /locus_tag="PK345_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HPX34563.1"
                     /translation="MVKTLFLVIIVFIVFDFIIEQLLAYLNSTRFSEVLPEEVKGIYD
                     EKEYIRSQQYLRLNNKFSILSDSVSFIILLAVLFFSGFGWLDHVVRQWSDNEIWQSLY
                     FFGIIAFVSDIISTPFSVYHTFVIESKFGFNKTTLKTFVLDKIKGWIIGIIIGGGLLA
                     LIIFIFQSTGQWFWILAWGLITLFSIFMSMFYSNLIVPLFNKQKPLEEGDLRTAIEEF
                     SKKAGFKLKDIYVIDGSKRSAKANAYFTGFGPKKRIVLYDTLIENHSIDELVAVLAHE
                     IGHYKKKHILTGMAMGIVQTGIMLFIFSIFISNPILSQALGAAQGSFHVGALAFGILY
                     SPVMLILEIATNAVSRKHEFAADRYAALNFNKQSLKEALKKLSVKNLSNLRPHPSVVF
                     VKYSHPPLLARLAAIDQA"
     gene            complement(3888..4469)
                     /locus_tag="PK345_05800"
     CDS             complement(3888..4469)
                     /locus_tag="PK345_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HPX34564.1"
                     /translation="MIPIDNTIVSDNLAEIKFVCDLKKCKGACCVEGDFGAPLLEEEI
                     GLIEDDIEVIKPFMTEAGIKTIDLYGVFDYDFSGEFVTPLVNGCECAFANFTDDIAWC
                     AIEKAYEAGVTRFRKPVSCHLYPVRISTRQSFDAVNYHEWGICRPALINGKRLGIPLY
                     KFLKEALIRRYGKTWYSKLEKKINTRNIKKDQA"
     gene            4832..5740
                     /gene="pyrB"
                     /locus_tag="PK345_05805"
     CDS             4832..5740
                     /gene="pyrB"
                     /locus_tag="PK345_05805"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HPX34565.1"
                     /translation="MKNRSLLSINDFSLDEQLKILTLAAEFEKNPTQKILEGFVVATL
                     FFEPSTRTKLSFESAASRLGAKVIGFSSLATSSVTKGESLKDTILTVSNYADIIVIRH
                     PNDGSARFAAEISPIPVINAGDGANQHPTQTLLDMYSILKTQGRLDNLNITFVGDLKY
                     GRTVHSLTIALTQFNTTFHLVSPVELKLPSYVKRHIKEKQLSYFQYTDLLDGIPQADI
                     IYMTRIQRERFSDPIEYEKVKNAYNLNNSMLINTKENMRILHPLPRVNEIALDVDNNP
                     KAYYFQQALNGVFVRQALLAAILGVK"
     gene            5769..6239
                     /gene="pyrI"
                     /locus_tag="PK345_05810"
     CDS             5769..6239
                     /gene="pyrI"
                     /locus_tag="PK345_05810"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HPX34566.1"
                     /translation="MEYSNQRKELKVSAIENGTVIDHIPANSVFRATRILRLDKMENQ
                     VLVGTYLDSKKYGKKGIIKVSNTFFKDDEINKIALVAPSATLIVIRDFKVVEKKQVEI
                     PKKIEKIVKCVNPNCITNHENIPTKFDVMKNENGIYLTCLYCDKNTSKENMVFL"
     gene            6379..6618
                     /locus_tag="PK345_05815"
     CDS             6379..6618
                     /locus_tag="PK345_05815"
                     /inference="COORDINATES: protein motif:HMM:NF025601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4342 domain-containing protein"
                     /protein_id="HPX34567.1"
                     /translation="MSKTNTFKVSGEELLKKIREIIHEGNVNRIIIKNEEGRTYLEIP
                     VTIGMIGAFIMPVFAAIGALAALAANFTIEVVRKE"
     gene            complement(6649..7788)
                     /locus_tag="PK345_05820"
     CDS             complement(6649..7788)
                     /locus_tag="PK345_05820"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPX34568.1"
                     /translation="MKKVLVFGAGLIGAPIAFDLAADDEFDVTVVDVSKINLTKFDGC
                     RKIKTWQRDLSDTNEVARIVKGFDFVLSAVPGYMGFNTLKAVIEAGKDVVDISFFSED
                     PFDLNQLAIEKGVVAIADMGVAPGMSNVLVGHACSELEEPTNVKIYVGGLPRIREWPY
                     EYKAVFSPVDVIEEYTRPARFVENGNCIIKPALSDPELLDFPCIGTLEAFNSDGLRSL
                     IKTIKCSNMIEKTLRYKGHIEIIKVLRDTGFFDKKPIDLNGTKISPLEFTSKILFPKW
                     KLNEGDEDLTVMRIIVEGVQKNKSIRYTYDLLDYYNTQTCVHSMARTTGYAATMALRT
                     ISNGLYESIGISTPEFLGKDKNCVDFMLNGLAQRGIYYTETIEEF"
     gene            complement(7785..8954)
                     /gene="wecB"
                     /locus_tag="PK345_05825"
     CDS             complement(7785..8954)
                     /gene="wecB"
                     /locus_tag="PK345_05825"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016662694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HPX34569.1"
                     /translation="MIKLLTVIGARPQIIKAAALSRAIRNSFSDRLQEIILHTGQHYD
                     RNMSQVFFDELEIPHPDFNLNVGSGAHGQQTALMIKGIEEHICKVKPQFIILYGDTNS
                     TLAGAVAASKAGVDVVHIEAGLRSFNKRMPEEVNRIVCDHVSTLLFTPTIAGYQNLLR
                     EGFSDNNSQPFNIDNPGLFHCGDIMYDNSLYFTTLAEKKSDILKKHNLQPQEFVLVTI
                     HRDLNTDNPIRLTAIFETLNKLAYSYKIDFILPLHPRTAKMINELLAKELIAQITKNR
                     YIKIIQPVSFLEMIMLEKAARMIITDSGGVQKEAYFFKKPSIILRPETEWIEIVENKV
                     GILANADPEKIENAFLNFVKSGNLDFPPLFGDGKAAEFICNLLIETYQFKIKSNS"
     gene            9210..10145
                     /locus_tag="PK345_05830"
     CDS             9210..10145
                     /locus_tag="PK345_05830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34570.1"
                     /translation="MIKYLRQDEIDVDKWDQCISHSVNGMIYAFSWYLNIVNNRWEAL
                     VEGDYERVMPLTPGKKANINYLFQPPFTQQLGIFSTSHLTSQVVNDFIKAIPSKYKLI
                     EINLNTYNKPDSQTTGFTPWLTHELDLISDYDTITKNYSNNLKRNLKKARQSKLSISE
                     HVRPEELITIFKENKGKELKHLTNYHYDLLRRLINVMVYKNTASVIGAYDETNSLCAG
                     AFFVFSNRKAIFYFSATNDTAKETGAMPLVIDYFIQKHAHSHITLDFEGSNDPGLARF
                     YKSFGATQITYPHLYLNKLNPLFRFGLSVIKKLKN"
     gene            complement(10166..11104)
                     /gene="queG"
                     /locus_tag="PK345_05835"
     CDS             complement(10166..11104)
                     /gene="queG"
                     /locus_tag="PK345_05835"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008773140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HPX34571.1"
                     /translation="MNGAELSHWIKKRSEELGFSACGIAPAGFLEKDAVRLEAWLKEG
                     MHGKMTYMEKHFEKRVDPRKLLDGAKSVIVFLYNYFPEKPLSEIDNFIISKYAYGQDY
                     HHVIKDKLYLMIKKISDLVGDINARPFVDSAPVLERSWAEKAGLGWIGKNASFIVPKK
                     GSFFFIAEIITDLELNYDFPRNNDFCGNCTLCIDACPTQAIVAPRIVDARKCISYLTI
                     ELKDEIPEEFRGALNDRIFGCDICQDVCPWTRFSKAHSEPLFTPNADLKKMKKDDWEA
                     LTPDVFNSIFRKSAVKRAKYTGLMRNIRFVGTDKNT"
     gene            complement(11107..11574)
                     /locus_tag="PK345_05840"
     CDS             complement(11107..11574)
                     /locus_tag="PK345_05840"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HPX34572.1"
                     /translation="MSFFEIGIYQSKKVENLGTLWRSAYQLGATGIFTINRRYQKQPS
                     DPFAVERHIPLRNYENFQQFLSQRPMNAILVAIEEGGTPLAEFHHPPQAIYLLGAEDS
                     GLPNVVLENCQLIVSLEAMIRPSYNVAVAGSIVMYDRVFGNKMGFPLPKSKMI"
     assembly_gap    11715..11724
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(11820..>13112)
                     /locus_tag="PK345_05845"
     CDS             complement(11820..>13112)
                     /locus_tag="PK345_05845"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HPX34573.1"
                     /translation="PEIFGDLKLYLGIVIVPFFWIILYIIVGSYRNVYRKTRVREVTI
                     TFISCFIGVLILFFTLLLDDVVVSPKDHYISFLVLFSFQLTFTLLFRLILTTRMVKKI
                     HHKIIGFNTILVGGGNNALELYQAMDSQFPSSGNKFVGFVTVKELDEYPLDKHLENLG
                     SYKSLAQLIEKMKVEEIIIAVERNESKAIEKVLSEVEITDVIIKLSPVMQDYMLGKVK
                     TTSIFTEPLIQISPSLLPPWQESLKRLIDFVASLVAVVLLSPVYLFLAIGVKLSSKGP
                     ILYQQERIGLHGKPFMIPKFRSMYVGAEKGVPMLSSKNDSRITPFGRFMRKLRLDEIP
                     QFFTVLKGDMSLVGYRPERQYFIDQIVEKAPHYRMLFKVKPGITSWGQVKFGYASTVD
                     EMVQRLKYDILYVENMSLAMDFKIMFYTLLIIVQGRGK"
BASE COUNT         4172 a         2690 c         2332 g         3908 t
ORIGIN      
        1 tcaattttta acatgactaa agctgttcaa cgggttatgt taaaacagcg taatggctca
       61 attatcaaca tgagctcagt agtggggctt tcaggtaatg ccggacaatc caactacagc
      121 gcttccaagg ccggccttat tggatttacc aaatccatag cacaggagtt aggctctcgt
      181 ggtgtccgtt gcaatgccat tgcgccaggc tttatcgaaa ctgccatgac caacaagcta
      241 tcggatgagg tacgcgaaaa ctggattaaa ggaatacctc tgcgccgatc gggtaagcct
      301 gatgatgtgg caaacattgc aattttcctt gcatcggagc tatcgtcata cgttactggt
      361 caggtgatca gtgtggacgg cggaatgcac atgtaaaagg tttaaatcat atttttaaag
      421 atcaaattgg tgaacattgc taccgaatct ccggcgtggt taatcatttt ctgcttactg
      481 gcaggattgg cgtacagtgc gttattgtat tataaaaata aaaaagaaga atttaacgca
      541 ttgacactcg gcattttatc agcttttagg tttatagcag tagccgtaat cgcctttctc
      601 ttactctcgc caatgctcaa aagtcatttc cgtaatttcg aaaaaccaat actcatcctt
      661 gcacaggaca actccgaatc actaattata ggtaaagatt cagcctttta ccgtaacgac
      721 tacccaaagg aattcctaaa gttagtggat gaacttcaaa aagagtacac tgtaagaact
      781 tacaattttg gccataaagt gagtgaagaa ctgactttcg attatacgga tcaacaaact
      841 gatatctccg aactttttga ggagataaac acacgatatt ccaatcgtaa tgttggaggt
      901 atgttattgg ccactgacgg cctgtataat agaggcatca atccggtgta tagcgctgaa
      961 aagttgaaat ttccaatctt cgcggtagct ttaggcgata caagcgttag aaaagatatc
     1021 tttatacgaa agataaatta taaccgtatt gcttttaaag gtaatgagtt cccgatcgag
     1081 gcaattgttg gggctaacct gtgtaatgaa gaaaatagca tactcactgt aagtcatggc
     1141 ggagcaatac ttttttcaaa aaacataaac ttcaactcta accatttcaa cgagactgtt
     1201 cacatcaccc taaatgccga gaaggcagga ttgcaacgtt atcgtataag tgtgaggccc
     1261 atcgaaggag agataagctt acaaaataac agtcaggata tttttattga ggtgttggac
     1321 acaaaacaaa aaatcatcat tctctctcaa tcgccgcacc ccgacattgc agcgctgaaa
     1381 gaggctattg aaagcaactg ggcttacgag gtaacacact ctttgtctaa ccagttttca
     1441 ggaaacatcg gtgaatataa cctgatcata ttacatcaac tgcccggcgg gcgggatgac
     1501 attagcagga tcatcgctga agccaacaaa caggcagtgc cattactttt tatcctcggt
     1561 tctcaatcca acctcgtaca attcaacaaa tccggggcgg gaatagaaat acaggcgcaa
     1621 aaggaactat tcaacgaatc actgccctcg cttaacaagg agttttcgct gtttaccatt
     1681 agtgatgatg tactcaaagc aatagagatg tttccaccgc tgatagcgcc attcggtgag
     1741 ataaaaacac aggtctcggc aaatactctc ttcaatcaac aaatcggatc gcttgtaacc
     1801 ggctaccctt taattgtttt caaccagact ttggataaca aaacaggggt tattgcaggg
     1861 gaaggaatct ggcgatggcg gcttatcaat tttcaaaaat atggaaacca caacgctttt
     1921 aatgaacttt tgtcgaaaat aatacagtac cttgcagtaa aggctgataa aagctttttc
     1981 agagtaacag gtcaaaatat ttttatggaa aatgaagcca tcctccttga cgccgaagtt
     2041 tacaaccaga gttacgagct tattaatact ccggatgtag agatcacctt aaaaaatagc
     2101 gttggcgaca catacaattt cctgttcgga aaaacctcca atgcatatca attaaatgct
     2161 ggtatccttc cgattgaaga ttatacttat gaggcaacag tgagagtagg cgaaaagcta
     2221 taccgtagcc aaggcgagtt caccgtttcg cctgtcaaca tcgaagcgat aaacacagta
     2281 gccgatcaca acctgcttta ccggttaacc tcaaaatatc agggcgaatt gatttatccc
     2341 gaacaactct ccacatttac caaaatgata aaagcacgtg atgatgtacg aacagtagta
     2401 tataccgaaa aaaaatttag tggattagtt aatatattcg gcatttttat ttttatagta
     2461 gccctgctaa cagctgaatg gtttattaga aaaagaagcg gatcatatta atatcttatt
     2521 ttagttttaa tgcgaactca attattgatc tgaaatgagg taagaaacga taagatttaa
     2581 tttcaaacct attaattttt cactaactct ttaattacaa aacccaatat ttcaccttcc
     2641 ataaataaca aacacttcta aactgtggtt aaaacacttt ttttagtaat aattgttttt
     2701 atagtctttg attttattat tgaacagcta cttgcttacc tcaactctac tcgtttcagc
     2761 gaagtactgc ctgaggaagt gaaaggtatc tatgacgaaa aagagtacat ccgttcgcaa
     2821 caatacctga gattaaataa taaattcagc attttatccg acagtgtctc atttataatt
     2881 ttacttgcgg ttctgttttt tagtggattt ggatggctcg atcatgtagt aagacaatgg
     2941 agcgataatg agatatggca atcgctttac tttttcggga taattgcttt tgtttcagat
     3001 attatttcta caccattttc agtctatcac acttttgtga ttgaaagtaa atttgggttc
     3061 aacaaaacca ctttgaaaac ttttgttttg gataaaatca aagggtggat tattggaatt
     3121 atcattggtg ggggtctatt agcgttgatt atttttatat ttcaatctac cggccaatgg
     3181 ttttggatat tggcttgggg cttaattact cttttcagca ttttcatgtc catgttttac
     3241 tccaacctta ttgtaccact gttcaacaag caaaagccac ttgaggaggg cgatctgcga
     3301 acggccatcg aagagttctc aaaaaaagcg ggttttaaac taaaggacat atacgttatt
     3361 gatggctcga aaagatcggc taaagccaac gcctacttta ccggctttgg accaaaaaaa
     3421 cgaattgtgc tttacgacac attgattgaa aaccattcta ttgatgaatt ggtagccgtt
     3481 ctggctcacg agatagggca ttataagaaa aaacacatat taacgggtat ggcaatgggc
     3541 attgttcaaa cgggaatcat gctttttatc ttttccattt tcattagcaa tccgatacta
     3601 tcacaagcgc ttggagctgc tcaagggagt tttcatgtag gagcgcttgc ctttggtatc
     3661 ctttacagcc cggttatgct gattttagaa attgcaacaa atgctgtttc gcgcaaacac
     3721 gaatttgctg ccgaccgata cgccgcactc aattttaata aacaaagttt gaaagaggcc
     3781 ttaaaaaaat tatcggtaaa aaacttaagc aacctacgtc ctcatccatc ggtagttttt
     3841 gttaaatatt cacatccccc cttgttagca cggttggcag caatagatca ggcctgatct
     3901 ttctttatgt ttcgagtatt aatcttcttc tcaagttttg aataccaggt ttttccatag
     3961 cggcgaataa gagcctcttt taaaaattta taaagcggta ttccaagtcg tttgccatta
     4021 atcaatgccg gacgacagat tccccactca tggtaattta cagcatcaaa agattgacgt
     4081 gtgctgatac gtaccggata aagatgacac gacacaggtt ttctaaatct tgttacccca
     4141 gcctcatacg ccttctcaat agcacaccag gcaatgtcat cggtaaaatt ggcaaaagca
     4201 cattcacagc cattaactaa cggcgtaacg aactcacctg aaaagtcata atcaaatacg
     4261 ccatacaaat caatagtttt gattccggcc tctgtcataa agggtttaat gacttcaata
     4321 tcatcttcaa tcaacccgat ttcttcctca agtaacggcg caccgaaatc accctcaacg
     4381 caacaagctc ctttgcattt tttaagatcg caaacaaact taatctctgc aagattatca
     4441 gatacgatag tattgtctat aggaatcatt tagcaaaaat atatttttaa ttcagatcaa
     4501 gcatttgaca atacttacat ggttaagtgt tcggacagtt caagaagagt tacactaaat
     4561 caggattagc cttagtaagt caatagtcaa tcccataaaa actttcaatt aattgattag
     4621 gactggcatg caagcgtgtg ttaataaaat attaaaaaat attcttcccc gccattgatt
     4681 tatttttaat tttgtttttt caaaaaggca caaatgttca cttttatatg cgagtttact
     4741 ttgtgttctc cgtttaagaa gtacttcctt ctcggggtac ttcttttttt ttatttatat
     4801 atatcatttt taaaataatt ttctcaagtc aatgaaaaac agaagtttac tctcgatcaa
     4861 tgatttttcg ttagatgaac aacttaagat cctgacgctt gcagccgagt ttgaaaaaaa
     4921 tcccactcaa aagattctcg agggttttgt tgtcgctaca ctctttttcg agccatcaac
     4981 acgtactaaa cttagttttg aaagcgctgc ttcgcgcttg ggggctaaag tcattgggtt
     5041 ttcttcatta gccacaagca gtgttacaaa aggagaatcc ttaaaagaca cgatcctgac
     5101 agtcagcaat tatgccgata tcatcgtgat acgtcatcct aacgacggga gcgctcgttt
     5161 tgctgccgaa atctcgccaa taccggtaat caatgccggc gatggggcta accaacatcc
     5221 tacccaaacc ctactcgata tgtattccat attaaaaaca cagggacgat tggacaatct
     5281 caacattaca tttgtcggcg atctgaaata tggacgcacg gtacattcgc taacgatagc
     5341 gcttacccag ttcaacacta cctttcactt agtctcccct gttgagctaa agctaccaag
     5401 ttatgtaaaa agacatatca aggagaaaca acttagctat ttccagtaca ccgatctgct
     5461 tgatggcatc ccacaagccg atatcattta catgacacgt attcaacgcg aacgattcag
     5521 cgacccgata gaatacgaaa aggtgaaaaa cgcttataac cttaacaact caatgctaat
     5581 caataccaaa gaaaatatgc gcatactgca cccattgcca cgtgtcaacg agatagcatt
     5641 agatgtggat aataacccaa aggcctatta cttccaacag gccctcaacg gcgttttcgt
     5701 aaggcaggcg cttcttgctg caatacttgg tgttaaataa attgtaatac tctttaatag
     5761 aaaaatatat ggaatacagt aatcaaagaa aagaattaaa agtatcggcc atagaaaatg
     5821 gcaccgtgat tgaccatatt cctgccaata gcgtttttcg ggcaacacgc atactccggc
     5881 tcgacaaaat ggaaaatcaa gtgttggttg gaacctacct tgacagtaaa aaatatggaa
     5941 aaaagggaat tattaaggtt agcaatacat tttttaaaga tgatgagatc aataaaattg
     6001 ctctcgtagc tccttcggca acactaattg tgatcagaga ctttaaggtg gtggaaaaaa
     6061 aacaggttga aattccaaaa aagattgaaa aaatagtaaa atgtgtaaac cccaactgta
     6121 tcacaaacca cgaaaatata cctaccaaat ttgatgtaat gaaaaatgaa aacggcattt
     6181 atttaacctg tctttattgc gataaaaata cttcgaaaga aaacatggtc tttctttaag
     6241 tatttcattc ataagccttt taactatttg gataattcgg caggtaatta attagtcgtc
     6301 ttttgcaggt ttacaccaca aacgatttcg attataaaaa ttaacaatat ctatcttttc
     6361 agttttaaag ctaaactcat gagtaaaacc aataccttta aagtaagcgg agaggagcta
     6421 cttaaaaaga tacgggagat aatccacgaa gggaacgtaa accgtatcat cataaagaat
     6481 gaagagggca gaacttatct tgaaattccc gtaaccattg gaatgattgg cgctttcata
     6541 atgccggtat ttgcagcaat tggcgcttta gcggccttag cggcaaattt tactatcgaa
     6601 gtggttagaa aagaataaaa tgggtattta tcagcaacac cgctcctttt aaaactcttc
     6661 aatagtttcg gtgtaatata ttcctcgctg tgccaaacca tttagcataa agtcaacaca
     6721 atttttgtcc ttacccaaaa attcaggcgt tgatatacct atactttcat aaagtccatt
     6781 cgaaattgtt ctcagggcca tggttgcagc ataacctgta gttcgtgcca ttgaatgaac
     6841 acaggtttga gtattgtagt agtctaaaag atcgtaagta taacggatag atttattttt
     6901 ttggacgcct tcaacaataa tccgcattac cgttaaatct tcatcaccct cattaagttt
     6961 ccatttcggg aacaaaattt tagaggtaaa ctccaaaggg cttatcttag ttccgttcag
     7021 gtctattggt tttttatcaa aaaatccagt atcgcgtaaa acttttataa tttcaatatg
     7081 tcctttatag cgtagggttt tttcaatcat gtttgagcat tttatcgtct ttatcaacga
     7141 tcgcaggcca tcgctgttaa aagcttcaag ggtaccaata cacggaaagt caagcaattc
     7201 aggatcggaa agcgccggtt tgataataca atttccattt tcgacaaacc gggctggcct
     7261 tgtgtattcc tcaatcacat caacaggtga gaatactgcc ttgtactcgt agggccattc
     7321 tcttatacgc ggtagtccgc caacgtaaat cttaacatta gttggctcct ccaattccga
     7381 acatgcatga ccaactaaaa cattgctcat acccggagct acccccatat cggcaatagc
     7441 aacaactcct ttctctatcg ccaattgatt gagatcgaaa gggtcttccg aaaaaaaaga
     7501 tatgtcaact acatctttcc ctgcctcaat gacagctttc aaagtattaa accccatata
     7561 tccaggcaca gccgaaagca caaaatcgaa ccctttgact attcgagcaa cctcgttggt
     7621 gtcggacaaa tccctctgcc aggtttttat ctttcggcat ccatcaaact tggtcagatt
     7681 tatcttacta acatcaacta cggttacatc aaattcatca tcagcagcca gatcaaaagc
     7741 tatcggcgct cctatcaaac cagctccaaa cactaatact tttttcatga attacttttt
     7801 attttaaact gataggtctc tataagcaaa ttacaaataa actctgcagc ctttccgtca
     7861 ccgaataacg gtgggaaatc aagatttcct gatttcacaa agttaagaaa tgcattttca
     7921 atcttttcag gatcagcatt ggctaatatt cccaccttgt tttcaacgat ctctatccat
     7981 tctgtttccg gccgaagtat gatagaaggt tttttaaaaa aataggcctc tttctgcact
     8041 ccccccgaat cagtgattat catgcgggca gctttttcca gcattatcat ctcaagaaag
     8101 gatacaggct ggataatttt tatatagcga tttttggtta tttgcgcaat caactctttt
     8161 gctaaaagct cgtttatcat cttagcagtg cgggggtgaa gcggtaagat aaaatctatt
     8221 ttataactat acgccagctt attcaatgtt tcaaaaatcg cggtaagccg aatcggatta
     8281 tcggtgttaa ggtctcggtg tattgttacc aaaacaaact cttgaggctg taagttgtgt
     8341 ttttttaaaa tatcagactt cttttctgca agtgtggtga aatatagact attatcatac
     8401 attatatcgc cacagtgaaa aagacctgga ttgtcaatat taaaaggctg tgagttatta
     8461 tccgaaaaac cttctcttag caagttttga tatccggcaa tcgtaggcgt aaaaagtagt
     8521 gttgaaacat gatcacaaac tattcgattt acctcctcag gcatcctttt gttgaacgat
     8581 cttaatcctg cttcgatatg caccacatca acacctgctt tagaggcagc cacagcgcct
     8641 gccaaagttg aatttgtatc gccgtaaaga atgataaact gaggttttac tttacaaatg
     8701 tgctcttcaa tgcctttgat catcaaagct gtttgttggc catgcgctcc agatccaacg
     8761 ttcagattaa aatcaggatg aggaatttcc agttcgtcaa aaaaaacttg agacatgttc
     8821 cgatcataat gctggcctgt gtgtaagata atctcctgta accgatcgct aaagctgttt
     8881 cggatagctc tgctcaatgc ggcagctttt ataatttggg gtctggcacc aataaccgtt
     8941 agcaacttaa tcatcatagt atttttttgg cgaaagtaaa aatattttag tttttataaa
     9001 aacgacttcc gccaatatat ttttgtcaaa ttacacattt gtaactgttt acagcggtta
     9061 tgcattgtta atctgcacaa ttaagattac tctaaaaaaa cgcaaaatcg ggattggatt
     9121 tataaaaccg acatacgatc ctgaaagatt ttctattgat taactatggt ttattaactt
     9181 tgacgccgta agtgataacc caaaacctta tgatcaaata cctaaggcaa gatgaaattg
     9241 acgtagataa gtgggatcaa tgcattagcc actctgtaaa tgggatgatt tatgcttttt
     9301 cgtggtacct caatatagta aataatagat gggaagccct tgtagaaggc gattacgaaa
     9361 gggtaatgcc acttacgcca ggcaaaaaag cgaatattaa ttatttgttt caacctcctt
     9421 ttactcagca gctcggtatt ttttcaacat cacacctaac ttcccaagtt gtaaacgatt
     9481 ttattaaagc aataccaagt aaatacaaac ttattgagat taatctcaac acatataata
     9541 agcccgattc tcagactaca ggtttcaccc catggttaac tcacgagctt gatctgatca
     9601 gcgattatga tactattaca aaaaattact ccaacaacct taaacgaaac ctcaaaaaag
     9661 ccagacaatc caagctttcc atttccgaac atgtcaggcc ggaagagttg attaccatat
     9721 tcaaggaaaa caaagggaaa gagctgaaac acttaaccaa ttaccattat gatcttcttc
     9781 gacggttaat taatgtaatg gtttataaaa ataccgcttc tgttattggc gcttacgatg
     9841 aaaccaacag tctttgtgca ggtgcatttt tcgttttcag caatcgaaaa gctatctttt
     9901 atttttcggc taccaacgat actgccaaag agacgggcgc tatgcctctt gtaattgact
     9961 atttcataca aaagcatgct cattcgcaca tcacactcga ctttgaaggc tctaacgatc
    10021 cgggacttgc cagattctac aaaagctttg gagcaacaca aataacctat ccgcatttat
    10081 atctcaacaa gctaaatccg ctctttcgat tcggtcttag tgtaattaaa aagctaaaga
    10141 attaaaaatc gaaaatagac tttgatcagg tatttttatc cgtacccaca aaacgaatgt
    10201 tcctcataag acctgtatat ttagcccttt taaccgctga tttccggaag atagagttaa
    10261 aaacatcagg cgttaaggct tcccaatcat cttttttcat tttcttcaga tcggcatttg
    10321 gagtaaagag cggctcgcta tgtgctttcg aaaatcttgt ccatgggcaa acatcttgac
    10381 agatatcgca tccaaaaatc cgatcgttta acgctcctct aaactcctcg ggtatttcat
    10441 ctttcagttc aatggtaaga taggatatac attttcgtgc atccacaatt cgtggagcga
    10501 caatagcttg tgtcggacat gcatcaatac agagagtgca attaccacaa aaatcattgt
    10561 ttcgtggaaa atcatagttt aattcaagat cggtgatgat ttcggcgata aaaaaaaacg
    10621 atcccttttt tggcacaatg aagcttgcat ttttcccaat ccaacctaat cctgcctttt
    10681 cggcccagga gcgttcgagc accggagctg agtcaacaaa aggacgagca ttgatatctc
    10741 ccactaaatc gctgattttc tttatcataa ggtaaagctt atccttgatc acatgatgat
    10801 aatcttgtcc ataagcatat ttactgatta taaaattatc tatttcggat agtggctttt
    10861 cggggaaata gttgtataaa aaaactatca ccgactttgc tccatcaagt agtttacgag
    10921 gatcaacgcg cttttcaaaa tgtttttcca tgtaggtcat ctttccatgc atcccttctt
    10981 tcaaccacgc ctctaacctg acagcatctt tttcaagaaa tccggctgga gcaattccac
    11041 aagctgaaaa gcccaattct tcagaccttt ttttaatcca atgcgacagc tcagcgccat
    11101 tcattattat atcattttag attttggcaa tggaaatccc attttatttc caaacacccg
    11161 atcgtacata acaatacttc cggcaacagc aacattgtat gatggccgta tcatagcctc
    11221 aagtgaaacg atcaactgac aattttccaa aacgacattt ggcaaacctg aatcttcagc
    11281 gcctaagaga tagattgctt gaggcggatg atgaaactcg gcaagtggag ttcctccctc
    11341 ttcaatggct acaaggatgg cgttcatagg tcgctgcgat aaaaactgct ggaaattttc
    11401 gtaattgcgt aaagggatat gtcgttccac agcaaaaggg tcagaaggtt gtttttggta
    11461 tctgcggtta atagtaaaaa tacccgtagc gccaagctga taagccgaac gccacagcgt
    11521 cccaaggttt tccacttttt ttgactgata aatccctatc tcgaaaaatg acatcttcag
    11581 cttttttgcg ctaaactaca ctgtttttca ctattgttta acaaaaaaaa ataaaactcg
    11641 ttgtcaatga gatactccaa atcattttaa cccaaagtat ttttgcaaag tatatgcatt
    11701 cttttcaaaa agctnnnnnn nnnnaccgtt ttgtttagtt ttatatatta gcatttttac
    11761 atttaattga aatgtagctc tccttaaaat accgccttta ttcttgacta caagtcatac
    11821 tattttcctc ttccctgaac aatgataagt aaagtataaa acatgatttt aaaatccata
    11881 gccaacgaca tgttttcaac ataaagaatg tcatatttca gtcgttgaac catttcatct
    11941 actgtggatg cataaccaaa tttcacttgt ccccacgagg ttatcccggg cttcactttg
    12001 aagagcattc gataatgagg cgctttttcc acaatctgat caataaaata ctgacgttcg
    12061 ggacggtagc caacgagtga catgtctcct ttgaggacgg taaaaaactg aggtatttca
    12121 tcaagcctga gtttgcgcat aaaacgacca aaaggagtaa tgcggctgtc gttttttgat
    12181 gagagcattg gaacgccttt ttcagcgcca acatacattg acctgaactt tggtatcata
    12241 aagggctttc cgtgcaatcc gatgcgttcc tgttggtata aaataggccc ctttgacgag
    12301 agctttacgc cgattgccaa aaataaataa acaggcgaaa gcaatacaac ggcaactaat
    12361 gatgctacaa aatcaatcag tcttttaagc gactcctgcc aaggtggcaa taatgaagga
    12421 gatatttgta ttaaaggctc ggtaaaaata gaggtagttt ttactttacc aagcatatag
    12481 tcctgcatta caggcgataa ttttataata acatcggtta tctccacctc cgaaagcact
    12541 ttctcgattg ctttactttc gtttctttcc accgcaataa ttatctcctc caccttcatt
    12601 ttttcaatca actgagccaa cgacttatat gacccaagat tctcaagatg tttatctaat
    12661 ggatattcat caagttcttt aacagtaaca aaaccaacaa atttattacc cgatgaaggg
    12721 aactggctat ccattgcctg ataaagctct aaagcattat tacctcctcc taccaggata
    12781 gtgttaaacc ctataatttt gtgatgtatt ttttttacca tcctcgttgt aagaataagc
    12841 ctaaacaaca aggtgaaagt taattgaaag ctaaaaagca caagaaaaga gatataatga
    12901 tcctttggcg atactaccac atcatccaga agcaaagtga aaaagagaat gagtacgccg
    12961 ataaaacaac tgataaaagt tatagttacc tctctaacac gcgttttacg gtaaacatta
    13021 cgataagacc cgactataat atataggata atccagaaaa aaggtactat tacaatacca
    13081 agatataatt tcagatcgcc aaatatctcg gg
//
LOCUS       DAOOAI010000064        12980 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_6187_length_12980_cov_5.547389, whole genome shotgun sequence.
ACCESSION   DAOOAI010000064 DAOOAI010000000
VERSION     DAOOAI010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12980
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6187_length_12980_cov_5.547389"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(21..314)
                     /locus_tag="PK345_05850"
     CDS             complement(21..314)
                     /locus_tag="PK345_05850"
                     /inference="COORDINATES: protein motif:HMM:NF018045.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bor family protein"
                     /protein_id="HPX34574.1"
                     /translation="MKKIVLSLALVFAVSVLMTSCYTYTFSVGEGAKTGVEIKERNHY
                     LIYGLAAIKTSDPTKMAGTAKDYTVTIEHTFIDGLLNALTGGLYTPTTTTVKK"
     gene            complement(486..1034)
                     /locus_tag="PK345_05855"
     CDS             complement(486..1034)
                     /locus_tag="PK345_05855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34575.1"
                     /translation="MKIRIGLIVIFTNCLTFVFAQNFWEQLYFPDTVTIRFLATNDSG
                     YAFMGAGNSQQTGDVYRSKDNAQSWELVYNNYGFSVQTIAISAVWNIYSRRTGSDRFL
                     FYEDNCDTWDVIELPPPSYGNIMKILCVGQDTICVSSWESEGAFIAKSFDTGGTWKHI
                     FVTDNQNGCVSDIAISSNYRVF"
     gene            complement(1354..3327)
                     /locus_tag="PK345_05860"
     CDS             complement(1354..3327)
                     /locus_tag="PK345_05860"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34576.1"
                     /translation="MKKAVLISLSLVISFAVFGQQKIKIKEGLKDCRVIERVYTNHSS
                     NGQLPKPFPASVPSGDRAVVLRQLGTSANGFGMLGVRQNLWADPAINAVSFIHRMHIP
                     PHGPGSGFLAYDLSLNGGETWTNNIQVYEAPSADFNGRYPQGGIYNPLGNTDPDNAYQ
                     AYFAAVLDGSNNGTWGGYGYGVHKFSGTEPPTQHNVAATPEFLQGVPEAYTITSKGLA
                     MCADPSNNGVGQPFADFFIITKGYFNPEIGDFEMDRFTEYMPAGGVSPVSGTAVGVAD
                     AKVAFSPDGEIGYISYLSNNGENTVESEGCYHPILYKTFDGGDSWDGPYNVQLGGASG
                     LPVVLEYLTDELIEILFEPPYPDREDIPFTSAFDHGLTVDFNGNPHLIFSVGIGSQDW
                     SIYTSHAGVEGCGGMVAMIHAFSTDKGETWYGKLLTLLKTFRGEFPMAGSDPSSEDNR
                     PYLASTPDGTKLFFSWIDTDIEDYGENDQPDIYCVGYDAINNTYSEVYNVSQFTAAWY
                     SSYMATGSKYALDMGDGTYKVPFAFQQIDPLDLGNPVQYWFVDNFILTDADLSVIQGT
                     NELEAFNFSVSQNYPNPCKGYTFIDVITKTKAAVSVEISNLIGQTVWKMPVKFYNEGK
                     HQLRLDIPELKKGVYLCNVQVDGKRISKKLIIN"
     gene            complement(3394..5028)
                     /locus_tag="PK345_05865"
     CDS             complement(3394..5028)
                     /locus_tag="PK345_05865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HPX34577.1"
                     /translation="MSEHSKNFASSSLDKSIRFFLWSIIISSVLIRGFLAWYVELGND
                     EVYYWTYALYPDWSHFDHPPMVGFFIWLTTLNLHFDSELFLRLSSVILGGVNIWLVYR
                     IGCKLKNCLTGLYAAIFFTGSVYCFVITGIFIMPDTPQLFFWLLSLMILIHVLPDRQL
                     TARSRQLLLLLGLTIGLAMLSKYTSAYLWVAIGIFILFYNPKWLKTTELYVAILISAV
                     MFLPVIYWNYKYNFISFTFHGERVNLFTSGVRPDLFFTELAGQILYNNPINFVVIIIA
                     LIALFRKKFSLNTDYKKILLLTSLPLAGLFLLFALFRPTLPHWSGPGYVSLILLSAIF
                     LAEKNKEKKLTIPVPAGLSVILLVVGLGIALAEIKTGFIGQPATDDPTKLGKNDVTLD
                     LSGWRNFGSQFRQLRLEDIESGEMSVASPIVVTRWFPAAHIDYYVAKPSGQNVIGIGE
                     LKNLHKYAWINRLRPELYPTSNAYYITSSRAYKAPDPSLLTYFDEISEPVIIKGFKGK
                     KHVQNFFVYRLKYCKSLPPDILESMGFERDLENKFD"
     gene            complement(5111..5773)
                     /locus_tag="PK345_05870"
     CDS             complement(5111..5773)
                     /locus_tag="PK345_05870"
                     /inference="COORDINATES: protein motif:HMM:NF014566.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II CAAX endopeptidase family protein"
                     /protein_id="HPX34578.1"
                     /translation="MRHKRDSLSSANRITLLITVAGVVLFSVMFIFKKTDWFGFWYWM
                     SANLIILLSFVFYTDSQNLKLLVNDFKENLVKKAILGLVFALILFMIFYFGNYLLGIL
                     YSGAGVEIKRVYDFKQNASDLRILILMLLIIGPGEELFWRGFVQRRLEIKKGKKSGLV
                     LATALYTVVHIFTGNIILLIAALTCGIFWGWLYQKYRSMIINMISHSLWDVMVFIILP
                     FG"
     gene            complement(5773..6732)
                     /locus_tag="PK345_05875"
     CDS             complement(5773..6732)
                     /locus_tag="PK345_05875"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HPX34579.1"
                     /translation="MPNDISMGFFKRWWISIRPFSLPASTMPVVFGSVLAYVYGNVSF
                     NFWFFLLAFFGMVFLHAGANILNDIFDYKNGFDKYPTLVSGGVVRKIITIKEAFIATI
                     GFLTIGALIGFLLAWLVGLKLLLIGIPGLLIGIFYTKGRKIALKYHALGDLAVFLNFG
                     IFGALGAWFVQTGTLSWIPVLWSMPIATLVIAILHANNWRDITSDADGHIYTIASVLG
                     DKKSLNYYGFLIFAPFFMVLALIFIPYFLFPEFPAMPFSFAIVLFSLPSALKLWQKAL
                     NRKQPKNPLDFIVLDGATAQYNLIFGLLCTGALVIESLFRNFF"
     gene            complement(6996..8198)
                     /locus_tag="PK345_05880"
     CDS             complement(6996..8198)
                     /locus_tag="PK345_05880"
                     /EC_number="2.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008776 - acetate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006085 - acetyl-CoA biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HPX34580.1"
                     /translation="MKIIVLNCGSSSIKYQLFEMPQVEVLAKGIVDKVGLKGALIKHE
                     RNDGKELKVEGEILDHKSGIEYLLGVLTDSEFGSLKSLKDIDAVGHRVVHGAEDFSGS
                     VAITHEVIRALEISTDLAPLHNPPNLKGIYAMQQLLPDVPQAGVFDTAFHQTMPEYSY
                     LYGIPYSLYEKHKIRRYGFHGTSHKYVSARACEILGEDIATKKIVTCHLGNGASMAAI
                     LYGKSVDTSMGMTPIEGVMMGTRCGDLDVGALFHMINKEDISSKIANTLVNKFSGVLG
                     VSGVSSDMRDVKKAADEGNHRAKMALQMYAYRVKKYIGAYAAAMGGVDIIVFTGGIGE
                     NDCDTRAKVIEGLEFLGVDFDFELNKGLRGKEVVLTSPSSKVKVLVVPTNEEMMIAKD
                     TYNIVTGN"
     gene            complement(8226..9200)
                     /locus_tag="PK345_05885"
     CDS             complement(8226..9200)
                     /locus_tag="PK345_05885"
                     /inference="COORDINATES: protein motif:HMM:NF014757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase family protein"
                     /protein_id="HPX34581.1"
                     /translation="MSITLDRFSLNKQLKVTGQIQKVGLIGCGAIGQEIALLISQKGI
                     EVVFIDLDEELIETILKSMSDQLDIIINRWGMTGSEKKLVMSRINGSIHYHDLADCDI
                     IIETINSQKPGANLEERLEVLRKVEAVVKPDTVITSNTATMMISDLAIALEHPERLLG
                     MHFISPVSRINLVEVVSHFHTNEASFLTVKKFAGMIGKKVIKINESPGNISTRMIVAI
                     INEACDILTEGVASVAEVDEVMMEVTGNTVGPFEMADRYGVDKIHKWMQNLYHEFGEL
                     KYKPSPIISRMVRAKMFGMRVGEGFYKWDGNMKSIKTGSIKNLGRDMF"
     gene            complement(9634..>12980)
                     /locus_tag="PK345_05890"
     CDS             complement(9634..>12980)
                     /locus_tag="PK345_05890"
                     /EC_number="3.1.21.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019812 - type I site-specific
                     deoxyribonuclease complex [Evidence IEA];
                     GO_function: GO:0009035 - type I site-specific
                     deoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HsdR family type I site-specific
                     deoxyribonuclease"
                     /protein_id="HPX34582.1"
                     /translation="TREEAVRREKELKTGFGRKWIKREIAAGRNKPFSTKEEEIEIRA
                     NKKTDTSTWHKGYISELETLILGMLNKETLIDLVRHFVVFEKSVSLRAPHRQEQEDPE
                     TKITTITTVKKLAAYHQYYAVNRAVESTLRAAGYHSLLLEGWTAKLDGVVHSPPLEGC
                     QTKSDGVVLSPPLEGCPKGGVVNKPDSYRDSQNYFDLPYNPNLKERARALRQAGNLSE
                     VLFWNQVKNKQFKGYDFDRQKIIGNYIVDFYCINCNVVIEIDGSSHDDKVEYDADRDA
                     YLESLGLTIIHIPAADVLQRMDAVMEMLFNHPALKDGRLMQDPPESYGLPGVKQQPIG
                     DRKGGVVWHTQGSGKSLSMVFYTGKIVLALDNPTVVVITDRNDLDDQLFDTFAASKQL
                     LRQEPVQAENRNQLKELLKVGSGGVVFTTIQKFQPDEGNVYEMLSDRENIVVIADEAH
                     RTQYGFKAKTIDDKNAHGEVIGKKIVYGFAKYMRDALPNATYLGFTGTPIESTDVNTP
                     AVFGNYVDIYDIAQAVEDGATVRIYYESRLAKVNLSEEGRRLVEEFDDELDQEDLTDT
                     QKAKAKWTQLEALVGSENRINQIAKDIIEHFELRQTVFEGKAMIVTMSRRIAADLYKA
                     IIKLRPEWHSDELNKGVIKVVMTSASSDGAEIAKHHTTKEQRRILAERMKNPDDELKL
                     VIVRDMWLTGFDVPCLHTLYIDKPMKGHNLMQAIARVNRVYKDKPGGLIVDYLGIAAD
                     LKKALSFYSDAGGKGDPTILQEQAVQLMLEKLEVVSQMFSEFPSFGGVDSEAGRGGTF
                     PSFGGVDSEAGRGGFNYEDYFDADTSKKLSMILAAEEHILSLEDGKKRYINEVTALSK
                     AFAIAIPHDQAMDAKDEVSFFQAVKARLVKFDSTGSGKTDEEIETTIRQVIDKALVTE
                     EVIDIFDAAGLKKPDISILSEEFLLELKGMKHKNIALEVLKKLLNDEIKLRAKKNLVK
                     SKTLKEMLENSIRKYHNKILTAAEVIEELINLSKEIINMDNEAKAMGLSDFEYAFYTA
                     VANNDSAKELMGKDKLRELAVVLTESIRKNATIDWTIKESVKAKLRVAVKRLLRKYGY
                     PPDMQKLATDTVLKQAELIANELTNQ"
BASE COUNT         3995 a         2798 c         2218 g         3969 t
ORIGIN      
        1 aaccctgact atagtaaaaa ctattttttt actgttgtcg tagttggagt ataaagtccg
       61 ccagttaaag cattcaataa accgtcaatg aatgtgtgtt ctatggttac ggtgtaatcc
      121 tttgcagtgc cagccatttt cgttgggtcg gaagttttaa ttgcagctaa accataaatt
      181 aaataatggt ttctctcttt aatttcaaca cctgttttag cgccttcgcc cactgaaaaa
      241 gtatatgtgt agcaagaagt cattaaaact gaaacagcaa acactaatgc taaacttaaa
      301 acaattttct tcatacttgt ttgatttaat tttttcctac tcataaggct tttcggatta
      361 cgccccgttt tttttagtgg gatctggtct ccactatcga agttcgataa taatattttt
      421 tgtcacgctg ggtggtcagc ccgttggttt ttgcagacca tctttatttt ttaaacttgc
      481 ccatttcaaa atactctata attgcttgat attgctatgt cagaaacgca tccattttga
      541 ttgtcggtta caaagatatg cttccatgtt ccacccgtat caaacgattt agcaataaat
      601 gccccctccg attcccatga actaacgcaa atagtatcct gacccacaca gaggattttc
      661 ataatattac cgtatgaggg tggtggtaat tcaattacat cccatgtgtc gcaattgtct
      721 tcataaaaca aaaatctatc ggaaccagtt cttctactat aaatattcca aaccgcacta
      781 atagctatag tctgaacact aaaaccataa ttgttatata ctagttccca actttgtgca
      841 ttatcttttg aacggtaaac atctcctgtt tgctggctat tgccagctcc cataaaagca
      901 tatccggaat catttgttgc taaaaatcga attgtaacag tatcaggaaa ataaagttgc
      961 tcccaaaaat tttgtgcaaa aacaaatgtt agacaatttg taaaaataac aatcagtccg
     1021 attcttattt tcattaatgt tggattttta cgattgtcaa aagtaataca ttattttttc
     1081 tgataatttt atttgtttca atattttttc ttaacggggc taattaatct cttttggttt
     1141 tttagcttct gatttttgct tcggcaaaaa ccaaatgtgc ttaaatgtga gataacattc
     1201 acattattgg taactaccag ggaggctttt cataggcttt ttttttgtaa aacaataaaa
     1261 aatgtacgaa gtcccgaaag acttcgtaca cttaaaacta tcataaccgt tgtaacaaaa
     1321 gctcgctcgg gcttttatta caactataaa ctattagtta ataatcagct ttttagaaat
     1381 tctttttcca tctacttgca cattgcatag atacacacct tttttcagtt cagggatatc
     1441 aagtcggagt tgatgtttac cttcattata aaactttaca ggcatcttcc atactgtttg
     1501 tccaatgagg ttcgaaatct caacgctaac ggcagcttta gtcttggtaa taacatcaat
     1561 aaaggtatat cctttacaag ggttagggta attttgcgat acgctaaaat tgaaagcctc
     1621 taactcgttt gttccttgta taacgctgag gtcggcatcg gtgagtataa aattatccac
     1681 aaaccagtat tgtacaggat tacctaaatc aagtggatca atttgttgaa acgcaaacgg
     1741 tactttatat gtaccatctc ccatatccaa agcatacttt gagccggtag ccatataaga
     1801 actataccag gctgctgtaa attgggaaac attatatacc tcggaatagg tattatttat
     1861 cgcatcatag ccaacgcaat agatatccgg ttgatcattt tcgccgtaat cttcaatatc
     1921 ggtatctatc cacgagaaaa atagttttgt accatctggt gtagatgcaa gataaggccg
     1981 attgtcttcg cttgatggat cgctgccggc catcggaaac tcaccacgga acgtcttcaa
     2041 taaggtgagc agtttaccgt accatgtttc acccttgtcg gtagagaaag catgaatcat
     2101 agctaccata ccaccacacc cctcaacgcc ggcatgtgaa gtatagattg accaatcctg
     2161 actaccaatt ccaacactaa aaatcaagtg tggattccca ttaaaatcaa cagtaagtcc
     2221 atggtcaaaa gctgaagtaa acggaatgtc ttcacgatca ggataagggg gttcgaaaag
     2281 tatctctatc aattcgtcag ttaaatattc taaaaccacg ggaagtccac ttgcaccgcc
     2341 taattgcaca ttataaggac catcccaaga atccccacca tcgaaagttt tataaagaat
     2401 aggatgataa cacccctctg actctacggt attttcgcca ttgttcgaaa gataggaaat
     2461 atatccaatc tctccatcag gggaaaaagc aactttggcg tctgccacac ctacagcagt
     2521 tcccgataca gggctgacac cgccagctgg catgtattcg gtaaaacgat ccatttcaaa
     2581 atcgccaatt tcaggattaa aatatccttt ggtgattata aaaaaatctg caaaaggttg
     2641 tccaacacca ttgtttgaag gatcagcaca cattgctaac cctttacttg taattgtata
     2701 ggcttcgggc acaccctgaa gaaactcagg tgttgctgca acattgtgtt gtgtaggcgg
     2761 ttcggtaccc gaaaatttat gaacgccata accatatcca ccccaggtac cattatttga
     2821 tccgtccagc actgctgcaa aataagcttg ataagcattg tctggatcag tattccccag
     2881 tggattataa attccaccct gcggataacg cccgttaaaa tcagcggaag gagcttcata
     2941 aacctgaata ttgttcgtcc aggtttcacc gccattaagc gataaatcgt atgcaagaaa
     3001 tcctgaccca ggaccgtgtg gggggatatg cattcggtga ataaaagata ctgcatttat
     3061 tgcaggatca gcccataggt tctggcggac gcctaacatc ccaaaaccat ttgccgatgt
     3121 tcccaactgc cttagcacaa cagccctatc gccgcttggc accgatgccg gaaagggctt
     3181 tggaagctgg ccattgctcg aatgattggt ataaacacgt tcaataactc ggcaatcctt
     3241 taaaccctct ttaattttga ttttttgttg cccaaatacc gcaaaagata ttacaaggct
     3301 taatgaaatt aatacagctt ttttcatagt aaattgtttt tatggtttat attattactc
     3361 cgtaaagata taattttttt taaataaaga cttttagtca aatttatttt caagatcccg
     3421 ttcaaaaccc atactttcta aaatatctgg aggaagagat ttacagtatt tcagcctgta
     3481 aacaaaaaaa ttctgcacat gctttttacc tttaaatcct ttgattatca cgggttcact
     3541 tatctcgtca aaatatgtta ataaggatgg atcgggagct ttgtatgcgc gactggaggt
     3601 aatataatat gcatttgacg taggataaag ttctggccgt aagcgattga tccaagcata
     3661 tttatgcaag tttttaagct ctcctatgcc aatcacattt tgccccgaag gctttgcaac
     3721 ataatagtca atgtgtgcgg ccggaaacca ccgagttaca actattggcg aggctaccga
     3781 catctccccg ctctcaatat cttcaagtcg aagttgcctg aactgactgc caaaattccg
     3841 ccaacccgag aggtctaatg taacatcgtt cttacctaat tttgtcggat cgtctgtagc
     3901 tggctgacct ataaaacctg tttttatctc cgccaaagca atacctaaac caactactaa
     3961 caaaatcaca cttaaacctg ccggaactgg gatcgttaat tttttttctt tgtttttttc
     4021 tgctaaaaat attgcagata aaaggattag cgaaacatac cccggaccgc tccaatgtgg
     4081 aagcgttggc ctgaagaggg caaaaagtaa aaaaagacct gccaggggca aagaggtaag
     4141 caataagatc tttttataat cggtgttcaa tgaaaatttt tttctaaaaa gagctatcag
     4201 ggcaataata attaccacaa agtttatcgg attattatat aaaatttgtc cggcaagttc
     4261 agtaaaaaag agatcaggtc taacacctga agtaaataag tttacacgct caccatgaaa
     4321 agtaaaactt ataaaattgt acttatagtt ccaataaatc accggcaaaa acattacagc
     4381 cgaaatcaag atagctacat acagttcagt agttttcagc catttaggat tataaaagag
     4441 aataaaaatc ccaattgcaa cccatagata tgctgacgtg tatttcgata gcatagccaa
     4501 ccctatggta aggcctaata gtaaaagcaa ctgacggctt cttgcagtca actgtctgtc
     4561 gggtaaaaca tgtattaata tcatcaggct cagcaaccaa aaaaataact gaggagtatc
     4621 aggcatgata aaaatgccgg taattacaaa acaatacacc gaaccggtaa aaaaaatggc
     4681 agcataaagc ccggttaaac agttttttaa cttacaacca attctataga ccaaccaaat
     4741 gtttactcct ccaaggatta ccgagctcaa acgcaaaaac aattcgctgt cgaagtgaag
     4801 atttagggtt gtcagccaga taaaaaagcc taccatcggc ggatgatcaa aatggctcca
     4861 atcaggataa agagcgtaag tccagtaata aacttcatca ttgcccaact caacatacca
     4921 ggcaagaaac cctctgatca gcaccgaaga tataattatt gaccaaagaa aaaatcgaat
     4981 gcttttatcc aaagaagatg aggcaaaatt tttagaatgc tccgacatag tttttgatca
     5041 atatttttct aaatattgtt caaagtaaaa gaaaaaaatg attacagaaa aaagagtttt
     5101 taaacaatta tcatccaaag ggaagtatga taaaaaccat gacgtcccat aatgaatgac
     5161 ttatcatatt tataatcatt gaacgatact tttgataaag ccatccccaa aaaatcccgc
     5221 aggtaagagc ggcaatcaga agaattatgt tgccggtaaa aatgtggact acagtatata
     5281 gggctgttgc taaaactaat cctgactttt ttcctttttt tatttctaac cttcgctgaa
     5341 caaaccccct ccaaaaaagc tcctcgcccg gcccgataat gagcaacata agaataagga
     5401 tacgaaggtc ggaggcattt tgcttaaagt cataaactct ttttatctca accccagcgc
     5461 cactatacaa aataccaaga agataattac caaaataaaa tatcatgaat agaatcaaag
     5521 caaaaactaa tcccagtatt gcctttttta caagattttc tttaaaatca ttgactaata
     5581 atttcagatt ttggctgtcg gtatagaata caaatgacaa gagaattata agattggcgc
     5641 tcatccaata ccagaaacca aaccagtcgg tttttttaaa aataaacatg accgaaaaga
     5701 gtactacacc tgcaacagtt atcagtaacg ttattctgtt agcagatgac agagaatccc
     5761 ttttatgcct catcagaaaa aattacgaaa caatgattca ataaccaaag cgcctgtaca
     5821 aagcaatcca aatatcaggt tatactgtgc tgtagcgcca tccaacacaa taaaatccag
     5881 agggtttttt ggttgttttc tatttaaagc tttttgccaa agttttaaag ccgaaggcaa
     5941 agaaaaaagt acaatagcaa aactaaatgg catagcagga aactccggga aaagaaaata
     6001 gggaataaaa ataagggcaa gcaccataaa aaagggtgca aaaatcagga agccgtaata
     6061 gtttaatgat ttcttatcac ccaatacgga tgcaattgta tagatatgac catccgcatc
     6121 gctggtaata tcacgccaat tattagcatg taggattgca attacgaggg tagcaatagg
     6181 cattgaccac agcaccggta tccacgataa tgttcctgtt tgtacaaacc atgctcctaa
     6241 agctccaaat atgccaaaat taaggaaaac agccaaatcg ccgagtgcgt gatatttcag
     6301 cgctatcttt cggcctttag tataaaagat tcctatcaaa agacccggaa tgccaatgag
     6361 aagtaactta agaccaacca gccaagccag caagaagcct atcaaagcgc cgattgttaa
     6421 aaatcctatt gtagcaataa aagcctcttt aatagttata atttttctga ctacacctcc
     6481 acttacaagg gttggatatt tatcaaaacc atttttataa tcaaaaatgt cattcaagat
     6541 attggccccg gcatgaagga aaaccatccc gaaaaaagcc agtaaaaaaa accagaaatt
     6601 aaaagagacg ttgccataaa cataagcaag caccgaccca aaaacaacag gcatagtaga
     6661 ggccggcaac gaaaagggac gaatactgat ccaccacctt ttaaaaaaac ccattgatat
     6721 gtcgtttggc attaattgaa gttttatatc aacacctgat cggacaagat tacctaatta
     6781 gacacatata cctcaaaaca ttaataacta acaaaaaaaa gctctctaaa attagtcctt
     6841 gtctgatttt gatttgagaa tagagatatg aattagtatt tgagttatca gaattattta
     6901 gccacaaagg catgaattca agttttttat ttgtgaattt gtaattagtt tgatgagcta
     6961 aacagggatt gaaacttcat aaaaaactat tttatttagt ttccggtaac gatgttgtag
     7021 gtgtcctttg caatcatcat ctcttcattt gttggcacta ccaaaacctt aaccttagat
     7081 gatggcgatg taagtacgac ctcttttcct cgtaagccct tattaagttc aaaatcaaaa
     7141 tccacaccca aaaattcaag accttcgatt actttagcac gggtatcgca atcgttttct
     7201 ccaattcctc cggtaaatac aatgatatct acgcctccca ttgcagcagc ataggcgccg
     7261 atatattttt ttacgcgata agcatacatc tgtagagcca tcttagccct gtgattaccc
     7321 tcatcagccg ccttctttac atcacgcata tcggatgata ctcccgaaac gcctaatact
     7381 ccgctaaact tattgactaa agtgtttgca atttttgagg atatatcttc tttatttatc
     7441 atgtggaaca aagcccccac gtcaaggtcg ccgcagcgcg tgcccatcat cacaccctct
     7501 atcggggtca tacccattga ggtatctacc gacttaccat acagtattgc cgccatcgaa
     7561 gctccattac caagatgaca ggttacaatt tttttagttg cgatatcttc tcctaatatt
     7621 tcacaagctc tggccgatac atatttatga ctggttccgt gaaacccata acggcgtatc
     7681 ttatgttttt cgtaaagcga ataaggaatt ccgtacagat acgaatattc cggcatagtt
     7741 tgatgaaacg ccgtgtcgaa gacacccgcc tgagggacat caggcaaaag ctgttgcata
     7801 gcataaatcc ctttgaggtt tggcggatta tgcagaggag ccaaatccgt ggagatttcc
     7861 aatgctcgta ttacttcgtg agttattgcc acactcccgc tgaaatcttc agcgccatgc
     7921 acaacacgat ggcctacggc gtcaatatcc tttaagcttt tcaggctacc aaattcagag
     7981 tctgtcaaaa cgccaagcaa atattctatc ccgcttttat gatccaatat ctcgccttct
     8041 acttttaact cctttccgtc gttacgttca tgcttgatca atgccccttt taggcctacc
     8101 ttatctacaa ttccttttgc aagcacctca acctgaggca tctcaaaaag ttgatattta
     8161 attgatgaac ttccgcagtt taaaacgata attttcattg tctcttttta ttacagttta
     8221 aatcattaaa acatgtcacg tcctaaattt ttgatactac cggtttttat tgatttcatg
     8281 ttcccatccc atttataaaa accttctccc acacgcattc caaacatttt tgcccttacc
     8341 atcctagaga taatcggtga aggtttatat ttcaattcac caaactcatg gtaaaggttt
     8401 tgcatccatt tatgaatttt atccactccg taacgatcgg ccatttcgaa tgggccaacg
     8461 gtattccctg taacttccat catcacctca tccacttcag ctaccgaagc aactccctca
     8521 gtaagtatat cgcaagcttc attgataatg gctactatca ttcttgtact tatgtttcca
     8581 ggcgattcat tgatttttat aacttttttg ccaatcattc ctgcaaactt ttttacagtg
     8641 aggaaggagg cttcattggt gtgaaaatgc gacacaacct ccaccaaatt aattcttgac
     8701 accggactta taaaatgcat gcccaacaag cgctccggat gctcaagtgc aatggcaagg
     8761 tcggaaatca tcattgtcgc agtattagag gtgatcactg tgtcgggttt tactactgcc
     8821 tccacttttc ttaaaacctc taaccgttct tctaagttcg ctccaggctt ctgcgaattt
     8881 atcgtttcga taataatatc gcaatcggca agatcgtggt aatggataga accgttaata
     8941 cgcgacataa ccaatttttt ttcactacct gtcattcccc agcggttgat tatgatatca
     9001 agttgatccg acatactttt gagaatagtt tcaattaact cttcatcaag atcaataaaa
     9061 accacctcaa tacccttctg actgataagc aaagctatct cctgcccaat agccccacaa
     9121 ccgataagtc caaccttttg tatctgacct gttactttca attgtttatt caggctaaac
     9181 ctgtctaatg taatggacat aaaaatttat ataaaattgt aaatcaaatt attaaagtag
     9241 aacctttagt tctatgtgtt tgaaatttct gcaaaattaa ccaaatgaaa gcttcaacct
     9301 aatgaaagta aattatttaa aatggatata aatttaaaat ttaataaaca tctgtggata
     9361 ttccggtttg atttgtgcca ccccaaaaga ccagtctatc ctatctcaaa aacaaacgag
     9421 ataagctatg gcaaataaac ttgatcctat ggttttgaaa caaattctaa ccttgcgcaa
     9481 agagggttgg agcaaccgca aaatagtgtt aacatctaat ttacaaagca aatttacaac
     9541 attcgtaaat ataaaaccaa taacataagc atgtcatttc aagacactat cccaatattt
     9601 atatcattaa aataaaattg tatctcaaat caattattga tttgttaatt cgtttgcaat
     9661 caattcggct tgctttaata cggtatcagt tgccaatttt tgcatatcgg gtggatagcc
     9721 atattttctc aacaatcttt ttacggcaac ccttagtttt gcttttacac tttccttgat
     9781 tgtccagtca atcgttgcat tcttcctgat tgattccgta agaacaacgg caagctctct
     9841 aagcttatct ttccccatta attcttttgc actatcgttg tttgcaacgg ctgtataaaa
     9901 ggcatattca aaatctgata atcccattgc tttcgcttcg ttgtccatat taatgatctc
     9961 tttgctgagg tttatcagtt cttcaatcac ctcggcagcc gtaagaattt tgttgtgata
    10021 tttccggatg gagttctcca acatttcctt taaagttttg cttttaacaa ggttcttttt
    10081 cgctctcaat tttatctcat cgttgagtaa ttttttcaat acttcgaggg caatgttttt
    10141 gtgtttcatt cctttcagtt ctaaaagaaa ttcttctgaa agtattgaaa tgtcgggttt
    10201 ttttaatcct gcggcatcaa aaatatcaat tacttcttcg gttaccaatg ctttgtctat
    10261 tacttgtcgg atggttgttt cgatttcttc atcggttttt cctgaacctg tactatcaaa
    10321 ctttaccaaa cgtgccttta ctgcctgaaa gaaagaaact tcatctttgg catccatagc
    10381 ttggtcgtga ggaatagcta tagcaaaggc ttttgacaat gccgtaactt catttatata
    10441 acgttttttg ccatcttcca aacttaaaat atgctcttcg gcggctaaaa tcattgaaag
    10501 ctttttggaa gtatcggcat cgaaatagtc ttcgtaatta aaaccacccc gtccagcttc
    10561 gctgtccacc cctccaaagg aggggaatgt accaccccgt ccagcttcgc tgtccacccc
    10621 tccaaaggag gggaattcgc taaacatttg tgaaactact tccagttttt ccaacatcaa
    10681 ctgaacagcc tgttcctgta aaatagttgg gtcgccttta cctccggcat cagaataaaa
    10741 cgaaagtgcc tttttcaaat cagctgcaat gcctaaataa tctacaatta aaccgcccgg
    10801 tttgtctttg taaacacggt ttacccttgc aatggcttgc atcaaattgt gtcctttcat
    10861 tggtttgtca atgtaaaggg tgtgcagaca tggcacatca aaaccggtga gccacatatc
    10921 ccgaacgata accagtttta attcatcatc cggatttttc atgcgctcag ctaatatacg
    10981 gcgttgttct ttggtggtat gatgttttgc aatctcggca ccatcggaag atgcggaagt
    11041 cattactact ttgattacgc ctttgttcaa ttcatcggaa tgccattcag gtcttaactt
    11101 gatgatggct ttgtataaat cggctgctat gcggcgactc atagtaacaa tcattgcttt
    11161 gccttcaaaa acggtttgcc gtaactcaaa atgttcgata atgtcttttg caatctggtt
    11221 aattcgattt tcactcccta ccaaagcttc taactgtgtc cacttggctt ttgctttctg
    11281 agtatccgtt aaatcctctt gatccagctc atcatcaaat tcttcaacta gtctcctacc
    11341 ctcttcgctc aaatttactt ttgccaagcg gctttcgtaa taaattcgaa cagttgcccc
    11401 atcttcaaca gcttgcgcaa tatcataaat atctacgtaa ttaccgaaaa cagcgggagt
    11461 atttacgtcg gtgctttcaa taggtgtgcc tgtaaagccg agataggtag cattcggcaa
    11521 tgcatcgcgc atatatttgg caaaaccgta aacaatcttt ttgccgatta cctcgccatg
    11581 tgcattttta tcgtcaatgg ttttggcttt gaaaccgtat tgggtacggt gtgcttcatc
    11641 ggcaatcact acaatgtttt cacggtcgga tagcatctcg tacacgtttc cttcgtcggg
    11701 ctggaatttc tgaatggttg taaaaacaac accacccgaa ccaactttta aaagctcttt
    11761 aagttggttt ctattttcgg cttgtaccgg ctcttgacgt aatagctgtt tagaagccgc
    11821 aaatgtatca aaaagctggt cgtccaaatc atttcgatcg gtaataacca caacggttgg
    11881 gttatccaat gccaatacaa ttttacccgt ataaaaaacc attgaaagtg attttccgct
    11941 tccttgggta tgccatacta caccaccttt gcggtcgcca ataggttgtt gctttacacc
    12001 aggtaaaccg taactttcag gaggatcctg cattaaacgt ccgtccttta gggcagggtg
    12061 gttaaaaagc atctccataa ccgcatccat tctctgcagt acatctgctg ccggaatatg
    12121 aataattgtt aatcctaaac tttccaaata agcatctctg tctgcatcat attctacttt
    12181 atcatcatgg ctgcttccat ctatttcaat cacgacattg caattaatgc aataaaagtc
    12241 aacaatataa ttgccaatta ttttttgcct gtcaaagtcg tatcctttaa attgcttgtt
    12301 cttaacttga ttccaaaaaa gtacttccga taaattacct gcttgacgca atgcacgtgc
    12361 gcgttcttta agatttggat tgtaaggaag gtcgaagtag ttttgagagt cgcggtagga
    12421 gtcaggttta ttaaccaccc cgcccttcgg gcacccctcc aaaggagggg aaagtaccac
    12481 cccgtcagac ttggtctgac acccctccag aggaggggaa tgtaccaccc cgtccagctt
    12541 cgctgtccac ccctccaaaa gaagggaatg gtagccggct gctcgtaatg tggattctac
    12601 agcacgatta acggcatagt attggtgata agccgccagt ttcttaacgg tagtgatagt
    12661 tgtgattttc gtttcgggat cttcttgttc ctgcctgtgc ggtgcacgca aactgactga
    12721 tttttcaaat acaacaaaat ggcggactaa gtcaatcagc gtctctttat tgagcatacc
    12781 caaaatcagg gtttctaatt cactaatata gcctttatgc catgtagaag tgtctgtttt
    12841 tttgttagct ctaatctcaa tttcttcttc ttttgtagaa aaaggcttat ttctgcccgc
    12901 tgctatttct cttttaatcc atttcctccc aaatccggtt tttaattctt tctctctgcg
    12961 gactgcttct tctctggttg
//
LOCUS       DAOOAI010000065        12877 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_6256_length_12877_cov_4.850725, whole genome shotgun sequence.
ACCESSION   DAOOAI010000065 DAOOAI010000000
VERSION     DAOOAI010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12877)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12877)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12877
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6256_length_12877_cov_4.850725"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            59..2842
                     /locus_tag="PK345_05895"
     CDS             59..2842
                     /locus_tag="PK345_05895"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HPX34583.1"
                     /translation="MKRHYLLLAFLFLTQYVFNQITTEPQFPIDSDEVLITFDATGTA
                     LENYTGDLYTHTGVILEGSNSWQYVIGNWGNNQTQPKLTRISDKQYTLNISPSIRSFY
                     NVPDNKIIRKMAFVFRSANANIQTVPDLFVDVYESGLSINITSPDKERIMVKENEIIP
                     VNAISPQADSIFLFVNNELRLAVEGIEIEYNLLAENFMGYWNDVDVNILAKKNEESAN
                     KSFYYFVIPLPDTATIPSGIEDGINYIDDNTVILSLYAPYKGYVFVLGDFNNWEYDKD
                     FYMKKTPDHNRYWIQINDLEAGKEYIFQYEVDGEIRIGDPFAEKVSDPWDDKYISNST
                     YPDLILYPTGMTTGIATVLQTAQQPYSWQINDFTPPAVEDMVVYELLIRDFTALHSYQ
                     SLSDTINYFKRLGVNVIELMPVSEFEGNISWGYNPNYYFAPDKYYGPKNTLKTFIDLC
                     HANGIAVVLDIVLNHSFGTSPYVMLYWDKVNKRPSANSPFYNPIAKHDFNVGYDFNHE
                     SQATKAFTKRVVNFWITEYKIDGFRFDLSKGFTQKNTLGNTAAWGQYDQSRINIWDEY
                     STYIWQIKPDAYVILEHFAENSEETVLANMGMLIWGNLNYNYNEATMGYHNNNKSDFS
                     WISYKKRGWNKPHVIGYMESHDEERLLYKNYMYGNSEAGYNIKDTTTALQRMELAANF
                     FFTIPGPKMIWQFGEYGYDVTIEQNGRTGQKPIRWQYLNDWRRKYLFNVYAALIDLKK
                     NHDVFKTDDFTLQVTDANKSIILRHNTMDVVIVGNFGTSASTITPGWTSLGKWYEFYS
                     QSEIEIDNDNSTINLAAGEYRLFSTQKIEKPEWLNTYIDEQNVNPDKVFFVYPNPSNG
                     EFHFSLNPDEQQRINLQIYTITGELVDEITHEISPTNPHLDWKPNKKLMPGIYFAKIR
                     SYEFNQTIKLLVK"
     gene            complement(3184..4302)
                     /locus_tag="PK345_05900"
     CDS             complement(3184..4302)
                     /locus_tag="PK345_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005636546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HPX34584.1"
                     /translation="MHLHIISFDIPYPPNYGGIIDVYYKLVALKRAGVKIHLHCYEYG
                     RKPAQELEQWCENVYYYPRSTGIWNSLYKRPYIVQSRRSKELINRLVQDEYPILFEGL
                     HSCYFIDDKRLKNRKKIYRESNIEHHYYYHLFKAERNLLRKPYFLLASLKLFIYQKIL
                     KHASLMLVVSQSDTLYLQQQFPGQRVVYLPSFHPNDEFSVISGRGYYALYHGKLSVME
                     NYQAARYLIEKVFRGSDYQLIIAGLDPPESLTRLAQKYVNVSVVPNPDDDTLFGLIRN
                     AHVNVLVTFQPTGLKLKLLNTLYQGRFCLVNKHMVTGTGLDQLCEIANSDKEMKIKID
                     EVFKREFKHSEVERRKELLKGVYSNKSKAEKLIKLVFE"
     gene            complement(4304..5419)
                     /locus_tag="PK345_05905"
     CDS             complement(4304..5419)
                     /locus_tag="PK345_05905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPX34585.1"
                     /translation="MKRAIVSVINDLVTDRRVDKTCNVLVQAGFDVLLVGRKLPDSLP
                     LPQRAYQMHRMGLLFKRGPLFYMEFNIRLFFLLLFKKVELLYSNDLDTLLPNYLLSRM
                     KSVPLVYDSHEFFTETPELVNRKFVQGIWKRIEKAIFPKLKDVITVNESISDLYYDLY
                     GVKVNVVRNISLAPVYESIKSREELGLPENIPIILLQGAGINIQRGAEEAIEAMRYLD
                     NVILLIIGSGDVIDILKHMKDEMGLGDKVIFLPKMPFDRLFHYTANATLGLTLDKDTN
                     INYRFSLPNKLFDYINAGTPVLASPLVEVKKIIDKYQVGETIENHDPKHIAKRITEMI
                     SDNEKLKVYRQNCHKASEKLTWQNEQKVLMKIFEKYA"
     gene            5586..8474
                     /locus_tag="PK345_05910"
     CDS             5586..8474
                     /locus_tag="PK345_05910"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HPX34586.1"
                     /translation="MQRFLEKTSRYICTQHQHNLADICVVLPNRRAGLFLKKIIPGIV
                     EKTTWAPDIFSIEDFIIHLSGFSLADQIDLLFTLHQIHQDIEKEDTHSFEDFLSWGQL
                     ILSDFNDIDLHLADAKSLFTYLSEAKAIERWNPSSATLSESVKKYLRFYQSLIHYYTS
                     LHGRCIANKSGYQGLIFKRVSGLIEQIIPNLKWQKVYFAGFNALTPSEQKIITTLVES
                     KKAEILWDADDYYVSNSMQEAGYFLRKEIEKNELKKFKWVDEGFRKIIKEVNLIGVPN
                     NLGQARYAASVIGNWANANTATADTLSNAALVLTDENLLFPVLNSLHDNTPKFNVTMG
                     LPIKLTVVFQLFNHIIRAFENADRFQSLNQNKAKGFYYSDIIVILQHPILFDLKGTNN
                     SLNKIKKAKRAFYRPDEITPLFDNYQQEIKEILLTIFGHLTPTVSQLIENMIFIIGKL
                     RKIINENVNEQSEAANPEIEYLFHLSGIFTRLQGLLAKHQICITLKAFKYLFNQIIQS
                     NRVPFEGEPLTGLQIMGLLETRTLDFEKVIILSVNEGIIPPTSTLNSIIPLDIRQTFG
                     LPGIMETNAVSAYHFYRLLQRAKEIHLVYNTEPDDLGGGEASRFIMQLEHELKQYQPA
                     HIISKKIIHQPIPDITFDNIIVIEKEEAVYRKLLDKAKQGLSPTSLNSFIECGLKFYF
                     SDIAGIKEEDEMEEFIESKTIGSIIHRTLELLYKPYINKALRADDINEMKQAAQQDIA
                     TAFADKYPGGEFTYGWNRLIKEVIHNFVEAFLKNEQKFLSAQKQLNRHLTILKLEHPL
                     NAIIHLDITGDADIKIKGIIDRVDQIEGKLRIIDYKTGKVEKNDLTFDTWESFAISEN
                     KSKAIQVLIYSWLYEKNYSQQEFPFISGIFALQKPSIGLLSFAIKQEINENQQYLVDK
                     TTLRKFEEILKEILTNLFDKSIPFTQTTNLNICQYCDFKKICQRLKLS"
     gene            8559..8948
                     /locus_tag="PK345_05915"
     CDS             8559..8948
                     /locus_tag="PK345_05915"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HPX34587.1"
                     /translation="MSQTKVRKQLINILKNSNVALSASDVARQMPATCDRVTLYRNFK
                     TLVEKGILHQITINNHESFYVLPQIDSKTNENNTEHIHFRCVKCSIVQCLHSSPIESI
                     NLPEGFSKLDTNFIVYGTCKNCNTISN"
     gene            8994..9404
                     /locus_tag="PK345_05920"
     CDS             8994..9404
                     /locus_tag="PK345_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013411.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HPX34588.1"
                     /translation="MKVTACRHTHFNATHRLHNPNWDDEKNEKVFGSCNNPNYHGHNY
                     NLIVKVKGEVDPETGYVMDMKVLKDIVKKHIEDRYDHKNLYLDVSDFADLNPSAENIA
                     IKIWNILREQIDSKFELSVVLYETDRNFVEYDGE"
     gene            9430..10620
                     /gene="yidD"
                     /locus_tag="PK345_05925"
     CDS             9430..10620
                     /gene="yidD"
                     /locus_tag="PK345_05925"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HPX34589.1"
                     /translation="MKRVLTKVFTLILVLPIRIYQYIISPLLPAACRHIPTCSEYAAH
                     ALKLHGPLKGGKLAANRILRCNPWGTSGVDPVPKFLIKKIKINTSIFTKQRFKTCNVL
                     KHNPRVIVLALVVSVVSFLASCASPENGKTTKPTVLVSIAPYKYFVEQIASDLADIMI
                     LIPPGTNHHDYDPSPRQLQALTKAKLLFINGGLPLEQNLVPSLRQNQPELKIINLSEG
                     INLIYDDDANNHNHHGHNYDPHIWLSFNNGLIIAKNVMTALLEMFPEHQERITANYNT
                     LKAKINEAIELNNVEMANLKCRQFVIFHPALSYYARDYNLEQISIEHEGKEPTPSQLK
                     KIIDIARKTGVQVVFIQKEFDASNARLVAESINGKIVQIDPLAEEWLDNLKAIASLIN
                     YPNP"
     gene            10626..11381
                     /locus_tag="PK345_05930"
     CDS             10626..11381
                     /locus_tag="PK345_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX34590.1"
                     /translation="MDKIIELQNVTAGYGEDIILRNVNIAIHRNDFIGVIGPNGGGKT
                     TLVKVILGLIKPITGKVIYYDSMKEGKQQKIGYLPQNSDTDKVFPISVLDVVLSGLAQ
                     PNRLINRFLKAQKAKAREILESTGILHLADKNFGELSGGEMQRVFLCRAIVSDPELLI
                     LDEPNTFVDNKFESDLYHTLKKLNEHKAIIMVSHDVGTIAYYVKSIACVNRELHYHQS
                     NIISQEQLDAYNCPIQIIAHGSVPHTILKEHNE"
     gene            11397..12224
                     /locus_tag="PK345_05935"
     CDS             11397..12224
                     /locus_tag="PK345_05935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018711202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HPX34591.1"
                     /translation="MTLFEYRFFTNALLAALFASITCGITGAYIVSRRIVFISGGITH
                     ASFGGIGIGYFFGFNPIVGAMIFSVLSALGIEYMSKKTNLREDSVIAILWSFGMAVGI
                     IFIFLTPGYSANLMSFLFGNILTVTKLNLVFLLILALLITGIFLVFFKTILFIAFDEQ
                     YARAVKLPVETINYLMITLIALTIVFNIKVVGIILVISLLTIPQTIANIFTKKFEIMI
                     WLSIIIGFLGSISGLLLSYYLNVPSGAAIIFFLVILFFISKIFRNILTSLRIRKQIQ"
     gene            complement(12425..>12877)
                     /locus_tag="PK345_05940"
     CDS             complement(12425..>12877)
                     /locus_tag="PK345_05940"
                     /inference="COORDINATES: protein motif:HMM:NF037435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5616 domain-containing protein"
                     /protein_id="HPX34592.1"
                     /translation="IVDGLNVIITIASYLQGLPVFIATDGLVRDASNVRAKIQTLNKL
                     TEATRLLKKYLPVLNIQNIEIYLDSQVESSMEISIILKEIDFTEGANVFIKLQKDVDR
                     SLIESSNGIVCTSDTGIIDKTDNRIFDLASAIIYHYYNPQILDLGQLG"
BASE COUNT         4198 a         2642 c         2289 g         3748 t
ORIGIN      
        1 ttaatatttt tcttattaac tttgcgcttc attaaaataa tctgtcaaat ttatgctaat
       61 gaaacgtcat tatctcctac ttgcttttct ttttttgact caatatgttt ttaatcaaat
      121 cactactgag ccacaatttc ccattgattc tgatgaagtg ctgatcacct tcgacgccac
      181 cggcacagca ttggaaaact ataccggcga cctatatact catacgggcg tgatattgga
      241 aggaagcaac agttggcaat atgttatcgg aaattggggt aacaaccaga cacagcctaa
      301 acttaccaga ataagcgata aacaatacac gctcaacatc tcgccctcaa tacggtcgtt
      361 ttataatgtc cctgataata agataatacg aaaaatggcc ttcgtgtttc gtagcgccaa
      421 tgctaatata caaacggttc ccgatctgtt tgttgatgtt tacgaatcag gcttgagcat
      481 taacataaca tctccggaca aagagagaat aatggttaaa gagaatgaaa tcattcccgt
      541 taatgccata agcccacagg ccgactctat ctttcttttt gtgaataatg aactcaggct
      601 cgctgtcgag gggatcgaga tcgaatataa ccttttagcc gaaaacttta tgggctactg
      661 gaacgatgtt gatgtgaata ttttagcaaa aaagaatgag gagagtgcca acaagtcatt
      721 ttactatttt gtaataccct tgcccgatac ggcgaccata ccctccggaa ttgaagatgg
      781 gatcaactat attgatgaca atacggtaat attatcgttg tatgcgcctt ataaggggta
      841 tgtgttcgtc cttggcgatt tcaacaactg ggagtatgat aaagactttt acatgaaaaa
      901 aactcccgat cataaccgtt actggataca aatcaatgat ctggaggccg gcaaagaata
      961 tatctttcag tatgaggtag atggtgaaat ccgtattggc gatccctttg ccgaaaaggt
     1021 aagcgacccg tgggatgata agtatatcag taattctact tatcctgacc tgatactcta
     1081 ccctaccgga atgaccaccg gcattgccac agtattacaa actgctcaac agccatactc
     1141 ttggcagata aacgacttta cacctccggc agtagaggat atggtagttt acgaattatt
     1201 gattcgcgat tttaccgcat tgcactctta ccaaagccta tccgacacta tcaattattt
     1261 caaaagattg ggagttaatg taatagagct gatgcctgta agcgaatttg aaggtaacat
     1321 atcatgggga tataatccta actattattt tgctcccgat aagtactatg gccctaaaaa
     1381 caccttgaaa acctttattg acctctgtca tgccaacggt atcgccgtgg ttttagacat
     1441 tgtgcttaac cactcctttg gcacatcccc ttatgttatg ttatactggg acaaagtcaa
     1501 caagcgccct tctgcaaata gtccgtttta taatccaatt gctaaacatg acttcaacgt
     1561 tggctatgac ttcaatcatg aaagccaggc aaccaaagct ttcacaaaac gagtggtcaa
     1621 tttctggatt acagaatata aaatagatgg cttccgattc gacctctcaa aaggctttac
     1681 tcaaaaaaat acgctcggca acaccgctgc ctgggggcaa tatgaccaat cgagaatcaa
     1741 tatatgggat gaatactcaa cctacatctg gcaaataaaa cctgatgctt atgtaatcct
     1801 tgaacatttt gccgaaaact cggaagagac tgttctggct aatatgggaa tgttgatatg
     1861 gggtaacctt aactacaact acaacgaagc caccatgggc taccacaaca acaacaaatc
     1921 agatttttca tggatatcct ataaaaaacg aggatggaac aaacctcatg ttataggtta
     1981 tatggaaagc catgatgaag agaggctact ctacaaaaat tatatgtacg gaaattctga
     2041 agccggatac aacataaaag atactaccac agctttgcaa cgaatggaat tggctgccaa
     2101 cttctttttt acaataccag gccccaaaat gatatggcag tttggagaat atggatacga
     2161 tgttactatt gagcaaaacg gacgaaccgg acaaaagcca atacgatggc agtatctaaa
     2221 cgactggcgc cgaaagtatc tttttaatgt ttatgcagcg cttattgacc tcaaaaaaaa
     2281 tcatgatgta tttaaaacag atgattttac gcttcaggta acagacgcta acaaatccat
     2341 tatcttacgc cataacacaa tggatgttgt aatagtgggt aattttggaa cttcggcctc
     2401 caccattaca cctggctgga caagccttgg aaagtggtat gagttttaca gccaatctga
     2461 aattgagata gataatgata actccactat taatctcgct gccggagaat accgactctt
     2521 ttcaactcaa aaaattgaaa aaccggagtg gcttaacacc tacattgacg aacaaaatgt
     2581 caaccctgat aaagtatttt ttgtttatcc caacccatca aatggagaat tccatttcag
     2641 cttaaaccca gatgaacaac aaagaataaa cctccaaatt tatacgatca ctggtgaatt
     2701 agtggacgaa ataacccacg aaatatcacc caccaacccg caccttgact ggaaaccgaa
     2761 caaaaaacta atgccaggca tctactttgc aaaaataaga agctacgaat tcaatcaaac
     2821 gattaagctg ctggttaagt aaaattattt tattggaaaa gaacaagttg ttctaaccaa
     2881 taatacacgg tttatttttt aatgattttg agatttgatg agctattttg gcttgaaata
     2941 ctcagaacat aaatacccgg agttttgcac ggagtattta tttcccctgt gttactctgt
     3001 gtagtttttt ttttttgtta cacagagttt ctcagtatga agcgatgctg ttaggcttgc
     3061 atccactttt atcattttac ttaccatcat caaacgctcc cccgaaagcc cgttctatat
     3121 ccctctataa gcagcatttt gaaaagatct aacccgctgt tgggtttttc aacacgaaga
     3181 aagctattca aacaccaact ttattagctt ttccgctttc gatttatttg aataaacacc
     3241 cttaagcagc tccttgcggc gttccacttc actatgttta aattcgcgtt taaaaacttc
     3301 atctatctta attttcatct ccttatcgct gttggcaatc tcgcacaact gatcaagacc
     3361 tgtccctgta accatatgct tattaactaa acaaaagcgt ccttgatata aggtatttaa
     3421 aagttttagc tttaatcctg taggctgaaa agtaaccaac acatttacat gagcatttcg
     3481 aataagtccg aaaagtgtat catcatcagg gttgggcaca accgatacgt taacgtactt
     3541 ttgcgccaga cgagttaagc tctcgggcgg atcaagcccg gcaataatca gttgataatc
     3601 gctgccgcga aatacttttt caatcaaata tctggctgcc tgataattct ccataaccga
     3661 taatttacca tgatacaaag cataatagcc acgtcctgaa ataactgaaa attcatcatt
     3721 aggatgaaaa ctagggaggt aaaccactct ttggccagga aactgctgtt gcaaataaag
     3781 ggtgtcgctt tgcgaaacaa ctaacatcag cgaggcatgt ttcaagattt tctgatagat
     3841 aaataatttt aagcttgcca aaaggaagta aggttttcgc aacaggttgc gctcggcttt
     3901 gaaaagatga taatagtaat gatgttcaat gttgctttcg cgatatattt tttttctgtt
     3961 tttcaatcgt ttatcatcaa tgaaatagca cgaatgcagt ccttcaaaaa gaattggata
     4021 ttcatcttgt accagcctgt tgatcaactc ttttgatctt ctgctttgga caatgtaagg
     4081 gcgcttgtaa agagaattcc aaattccggt ggagcgaggg taataataga cattttcgca
     4141 ccactgttca agctcctgag ccggcttgcg gccatattca taacaatgca ggtgaatctt
     4201 aacccccgcc ctcttcaagg ctacaagctt ataataaaca tcaatgatac ccccataatt
     4261 gggtggatat gggatgtcga aagagataat gtgcagatgc atgtcaggca tatttttcaa
     4321 aaattttcat caacaccttt tgttcatttt gccaagtaag tttttcagaa gctttatggc
     4381 agttttggcg atatactttc agtttttcat tatccgatat catctccgtt attcttttgg
     4441 ctatatgttt cggatcgtga ttttctatag tttcccctac ctgatacttg tcaattatct
     4501 ttttcacttc aaccaaaggc gatgctaaca ccggcgttcc ggcatttata taatcaaata
     4561 gcttgttagg gagcgagaat ctatagttga tgttggtgtc tttatcaagc gtcaagccta
     4621 atgtagcatt agcagtataa tgaaacaaac ggtcaaaagg catttttggc agaaaaatta
     4681 ccttatcgcc caatcccatt tcatctttca tgtgtttcaa aatatcaatt acatcgccac
     4741 tgccgataat cagcaagatg acattatcca aatatctcat agcctctatc gcttcctccg
     4801 caccacgctg aatatttatc ccggctccct gaagcaatat tatcggtata ttttccggaa
     4861 gcccaagctc ttcacgactt tttatacttt cataaaccgg cgccaacgaa atgttacgca
     4921 ccacatttac ctttacccca tacaaatcat agtaaagatc agaaattgat tcgtttaccg
     4981 taattacatc tttaagcttg ggaaaaatcg ccttttcaat tcttttccaa attccttgta
     5041 cgaactttcg gtttaccaac tccggtgttt ctgtaaaaaa ctcatggcta tcatacacca
     5101 atggcaccga cttcatccgt gataaaagat aattgggtaa aagtgtatcg agatcgttag
     5161 aataaagcag ttccactttt ttaaataata ataaaaagaa caatctgata ttgaactcca
     5221 tataaaacaa cgggccgcgc ttaaagagga gtcccattcg atgcatttgg taagcacgtt
     5281 gcggaagcgg cagactatcc ggcagcttac gaccaaccaa cagcacgtca aaacccgcct
     5341 gaacaagtac attacaggtc ttatctaccc tacggtcggt aacaagatcg ttaattacag
     5401 aaacaatagc tcttttcaat tacgctgcat ttatttatgc gaaagtaagc atttgataat
     5461 tacttaaaaa tctattgatc atcaattaaa ccgaaacctc ttgatcatcg gcctaaaact
     5521 tattttaacc gctaaaagaa aaatgacaaa aatcattact tttgaaccaa tatattccgt
     5581 ttattatgca acgatttctt gaaaaaacaa gtcgctacat ttgcacacaa catcaacaca
     5641 atctggctga tatctgtgtt gttctgccaa atcgcagggc tggtttattt ttgaaaaaga
     5701 tcattccggg tatcgttgaa aaaactacat gggcgccaga tattttttct atcgaagatt
     5761 ttattattca cctttccggt ttcagtctgg ccgaccaaat tgacctgctc tttactcttc
     5821 atcaaattca tcaggacata gaaaaagagg atacccactc atttgaagat ttcctttcgt
     5881 ggggtcaact tattttaagc gattttaacg atatagattt acatcttgca gatgcaaaat
     5941 ctctatttac ctatctctcc gaagccaaag ctattgaaag atggaatcca tcaagcgcca
     6001 cgctttccga atctgtaaaa aaatatctac gtttctatca atcactgatc cattactaca
     6061 caagcctaca cggtcgttgt atagcaaaca aatcgggcta ccagggattg atattcaaaa
     6121 gggtttccgg acttatcgaa caaatcatac caaatttgaa atggcaaaag gtctatttcg
     6181 caggttttaa tgctttaacc ccttctgagc aaaaaattat caccaccttg gttgaatcca
     6241 agaaagcgga aatactttgg gatgctgacg actactacgt ttccaactca atgcaggagg
     6301 ccggttactt tttaagaaaa gagattgaaa aaaacgaact taaaaagttt aaatgggtgg
     6361 acgagggatt tagaaagatc attaaagagg taaacctaat aggagtaccc aataatttag
     6421 gacaggcgag atatgctgca agtgtcattg gcaattgggc aaatgcaaat accgcaacag
     6481 cagatacact aagcaatgca gcacttgtgc tgacggatga aaaccttttg tttccggtac
     6541 taaattcgtt gcacgacaac acaccgaaat tcaatgtaac aatgggtttg ccaattaagc
     6601 tgactgtcgt ttttcaactc ttcaaccaca ttatacgggc ctttgagaat gctgacagat
     6661 ttcaatcgtt aaaccaaaac aaagccaaag gtttttatta ttccgatatc atcgtaatcc
     6721 ttcaacatcc cattcttttc gatttaaaag ggacaaataa tagtctaaat aagataaaaa
     6781 aggctaagag agccttttac cggccagacg aaataacacc tctatttgat aactatcaac
     6841 aagagataaa ggagatactg cttactattt ttggccacct cactcctact gtaagtcaac
     6901 tgatagaaaa catgatattt atcattggca agcttcgcaa aataatcaat gaaaatgtta
     6961 atgagcaaag tgaagcggca aatcccgaaa ttgaatatct gtttcacctt tccggtatct
     7021 ttacccgttt acagggtttg ctcgccaaac atcagatttg cattacgctg aaagctttta
     7081 aatacctttt caatcaaatt atacaaagca acagggtacc ttttgaaggc gagcctctta
     7141 cagggctaca gattatgggg ttattggaaa cccgtactct tgatttcgaa aaagtgataa
     7201 tcctgtcggt caacgaaggc attatcccac ccacttcaac cttaaactcc atcatcccgc
     7261 tcgatataag acaaacattc ggcttgccgg gaataatgga aacaaatgcc gtttcggcat
     7321 atcattttta tcggctgtta caaagggcaa aagagataca tttagtatat aatactgaac
     7381 ccgacgatct tggcggtggt gaagcaagtc gttttataat gcagttggaa cacgaactga
     7441 aacaatatca accggcacat atcatttcaa aaaagataat tcaccaacca atacctgata
     7501 taacattcga caatataatt gtgatcgaaa aggaagaggc ggtttataga aaattattag
     7561 ataaagcaaa acaaggactt tcgccaactt cactcaacag ttttattgaa tgcggtttga
     7621 agttttattt cagcgatatt gccggcatta aagaggaaga tgagatggaa gaatttattg
     7681 agtcgaagac aatcggaagc attatacacc gaacgcttga acttttatat aagccatata
     7741 ttaacaaagc tcttcgagct gatgacatta acgagatgaa gcaagcagca caacaggata
     7801 ttgcaacagc atttgccgat aagtaccctg gaggcgagtt cacctatggc tggaacaggc
     7861 taattaagga ggtaatccat aattttgttg aagctttctt aaaaaacgaa caaaagtttt
     7921 tgtcggctca aaaacaactc aatcgccatc ttactattct caaacttgaa cacccgttaa
     7981 acgcaataat tcacttagat ataaccggtg atgcagatat aaagatcaaa ggaataattg
     8041 accgcgtaga tcagatagag gggaaattgc gtatcataga ttacaaaacc ggcaaagttg
     8101 aaaaaaatga tttaaccttc gacacatggg aatcgttcgc tatctccgag aacaaaagca
     8161 aagctattca agtactgata tattcatggc tctacgaaaa aaattattct caacaggaat
     8221 ttccgtttat ttcaggaatt ttcgctttgc aaaaaccctc cattggcttg cttagttttg
     8281 ctataaaaca agagatcaat gaaaatcagc agtatttagt tgataagacg actttgagaa
     8341 agtttgaaga gattttaaaa gagatactca ctaacctgtt tgacaaatca atccccttta
     8401 cacaaacgac aaacctcaac atttgccaat attgtgattt caaaaagatc tgtcaacgtt
     8461 tgaagctttc ctaatggatt tagaaataaa gttttatttt tgcaacaatg ttgcataaat
     8521 cagatatcag cacttcagaa cttcttaaaa aacataactt gagtcaaacg aaagtcagga
     8581 agcagctcat caatattttg aagaacagca atgtagctct ttcggcatcg gatgttgcca
     8641 gacaaatgcc ggcaacctgc gatagggtaa ctctttatcg taatttcaaa actttggttg
     8701 aaaaaggtat attgcaccag ataaccatca ataaccatga atcattttat gttctgccgc
     8761 aaattgattc gaaaaccaac gaaaacaaca ccgagcatat tcattttaga tgtgtaaaat
     8821 gcagtatcgt tcaatgtctt cacagcagcc cgatagagtc aatcaatctg ccggaaggtt
     8881 tttcgaagtt agatactaac tttattgttt acggaacctg taaaaattgt aatacgattt
     8941 caaattaata atttaaagat tttaaaagtg aaaattaaac agagtgatac accatgaaag
     9001 taacagcttg ccgccataca cattttaacg ctactcaccg tctgcacaac ccaaactggg
     9061 atgacgaaaa gaatgaaaaa gtctttggct catgcaacaa ccccaactac catggtcata
     9121 actataatct aatcgtcaaa gtgaagggag aggttgatcc tgagacaggt tatgtgatgg
     9181 acatgaaagt attgaaagat attgttaaaa agcatattga agacagatat gaccataaaa
     9241 atctctattt ggatgtgagt gattttgccg acctgaaccc aagcgctgaa aacattgcaa
     9301 tcaaaatatg gaatatattg cgcgaacaga tagactcaaa atttgagcta tcggtagtac
     9361 tttacgaaac tgatcgaaat tttgtggaat acgatggcga gtaacagtaa ctataaattg
     9421 actttgcaaa tgaaaagagt actaacaaaa gtctttactc taatccttgt actgccgatc
     9481 aggatttatc aatacattat ttcacctctt ctaccggctg catgcaggca cattcccacc
     9541 tgctcggagt atgccgccca cgctttaaaa cttcatggcc cgttgaaggg tggaaaattg
     9601 gcagccaacc gcattttgcg ttgtaaccca tggggaacct caggcgtgga ccctgtccct
     9661 aagtttttga taaaaaagat aaaaataaac acatccattt ttacgaagca acggtttaaa
     9721 acctgcaatg tgctaaaaca caaccctcgt gttatagttt tagcgcttgt tgtatcggtc
     9781 gtatcatttt tagcttcatg tgcatcacct gagaatggca aaactaccaa acccacagtg
     9841 ttagtaagca ttgctcctta caaatatttt gttgaacaaa tcgcaagcga ccttgccgac
     9901 ataatgatct taataccgcc aggaaccaat catcacgatt atgacccgtc gccaaggcaa
     9961 ctgcaagcat taacaaaagc taaactctta ttcattaacg gaggacttcc tttggaacaa
    10021 aatttagttc cctcattgcg ccaaaaccaa cctgaattaa agatcatcaa ccttagtgaa
    10081 ggcataaacc ttatatatga tgatgatgct aataatcata atcatcatgg ccacaactac
    10141 gatcctcaca tttggctctc gttcaataat gggcttataa tagcaaaaaa tgtgatgaca
    10201 gcgcttttgg aaatgtttcc tgaacatcag gagagaataa cagccaacta taacacactt
    10261 aaggcaaaaa taaatgaggc tatcgaactt aacaatgttg agatggctaa tcttaaatgc
    10321 cgacagtttg taattttcca tccggcattg agctattatg cccgcgacta taacttagaa
    10381 cagatatcaa ttgaacacga aggcaaagaa cctacgccat cgcagcttaa aaagataatt
    10441 gatattgccc gaaaaaccgg tgtacaggtt gtgtttattc aaaaagagtt cgacgcttcc
    10501 aatgcaagat tagttgccga atcaattaat ggaaaaatag tgcagattga tcctcttgcc
    10561 gaagagtggc tggataacct taaagctatt gcctccctta tcaattatcc aaatccttaa
    10621 atacgatgga taaaattatt gaattacaaa atgttacagc aggatatggc gaggacatca
    10681 ttcttcggaa tgtgaacatc gccattcatc gcaacgactt catcggggta ataggcccta
    10741 acggtggtgg aaaaacaaca ttagttaaag taattctggg acttattaaa cctattaccg
    10801 gaaaagtaat ttactatgac agtatgaagg agggtaagca gcaaaaaata gggtatctgc
    10861 cacaaaatag tgatacagat aaagtttttc ctatttccgt gttagatgtc gtgttatccg
    10921 gtcttgctca acctaatagg cttatcaatc gttttttgaa agcgcaaaaa gctaaggcgc
    10981 gtgagatact tgaatcaaca ggcatcttgc atctggccga caagaatttc ggcgagctgt
    11041 cgggcggaga gatgcagcgc gttttccttt gtagagccat tgtatccgat ccagagcttc
    11101 ttatccttga cgaacccaac acctttgttg ataataaatt tgaaagcgac ctctatcata
    11161 ccctaaaaaa attaaacgag cataaggcga tcatcatggt gtcgcatgat gtcgggacaa
    11221 tcgcgtatta cgtaaaatca atagcctgcg taaacagaga acttcattat catcaatcca
    11281 acatcatttc gcaagaacaa ttggatgcat acaactgccc gatacaaata attgctcacg
    11341 gcagtgttcc gcatactatc ctgaaagagc ataatgaata gactatggaa acaattatca
    11401 ctctttttga atatcgtttt tttaccaatg ccctacttgc agccctattt gccagcatca
    11461 cctgtgggat aaccggcgct tacatagtct ccagaagaat cgtattcatc agcggaggga
    11521 tcacacatgc atcgtttggc ggaataggga taggatactt ttttggtttt aatcctattg
    11581 taggcgcaat gatcttcagt gtgttatcag cgcttggcat tgaatatatg tcaaaaaaaa
    11641 ccaacctacg cgaagattcg gtaattgcca ttttatggtc gttcggtatg gcagtcggga
    11701 ttatctttat ctttcttaca ccaggatatt cagccaatct gatgagcttt ctttttggca
    11761 acatacttac tgttacaaaa ttaaaccttg tcttcttatt gattttagcc cttttaataa
    11821 ccggcatctt tctggttttc tttaaaacaa tattgttcat tgccttcgac gaacaatatg
    11881 cacgagccgt taaacttccc gtagaaacta tcaactatct gatgattaca ttaatagcgc
    11941 tgacaattgt atttaatata aaagtcgttg ggatcattct cgtaatttcg ctgctcacga
    12001 tcccacaaac catcgccaac atttttacaa agaaatttga aataatgatt tggctctcca
    12061 tcattatagg atttcttggc agtatctcag gcctattact ctcttattat ctgaatgtac
    12121 catcgggcgc cgcaatcatc ttctttcttg taatcctatt tttcataagt aagattttca
    12181 gaaatattct aacttcattg cgcatcagaa aacaaattca atagcatcta tcacatttac
    12241 ctaagtcagc aaaacaattt gccataacct gcttagcgcc gctaatttaa ttccgccctc
    12301 ggataaagtt accaaatcac caatttgctt tttaatcgta tattaatatc caccggtatt
    12361 gttttattaa taaaaaccat tgccgaaatt taaaaagttt tataaggaaa cagcgcttac
    12421 cagattatcc taattgtcca agatccaaaa tttgtgggtt gtagtaatgg taaattatgg
    12481 ccgaagccaa atcaaaaatt ctattatccg tcttgtcaat tattcctgtg tcggaagtgc
    12541 atactattcc atttgaactt tcaataagtg atctatctac atctttctgt aatttgataa
    12601 aaacatttgc accttcggtg aagtctatct ctttcaaaat aatgctgatc tccatacttg
    12661 attcaacttg actgtcgaga taaatttcaa tattttggat attcagtacc ggtaaatact
    12721 ttttcagaag ccgagtagcc tccgtcagtt tatttaaagt ttggattttg gctcgaacgt
    12781 tggaagcatc tcttaccaat ccgtctgtgg caataaagac cggtaacccc tgcaaataag
    12841 aggctatggt gataataaca ttaagcccgt caacaat
//
LOCUS       DAOOAI010000066        12844 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_6288_length_12844_cov_5.356478, whole genome shotgun sequence.
ACCESSION   DAOOAI010000066 DAOOAI010000000
VERSION     DAOOAI010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12844)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12844)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12844
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6288_length_12844_cov_5.356478"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(101..1351)
                     /locus_tag="PK345_05945"
     CDS             complement(101..1351)
                     /locus_tag="PK345_05945"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPX34593.1"
                     /translation="MNKKLLILTISALVLIFAVWYFTKKNTKSVEHIKVPVKFGMFIV
                     DVSTTGELGAKSSENIFGPGNLLSLEIWGDLKINKLIPEGTLVDSGDYVASIDQTEVM
                     SKLKDIETELEKLESQYTKTMLDTSLTLRAARNDLINLKFGLEEQQIRVEQSIFEPPA
                     VQRQEEINLDKANRAYDQAVENYLLRLEKSKAEMQEVSATLEQARRKRQNMLEILKKF
                     TIYAPQSGMVIYKRDWRGRKTETGSTINQWENVVALLPDLSKMITKTYVNEIDISKVR
                     VGQPVKISIDAFPDREYNGIVTEVANIGEQRPGSDAKVFEVVISVDETDTIMRPAMTT
                     KNVIITEQVDSVLYIPIEALHSNDTINFVFQETGMRTIKKQVRVGQSNNNEIIILEGL
                     KQGDEVLLNFPENHADLQLTPLNK"
     gene            complement(1360..2328)
                     /locus_tag="PK345_05950"
     CDS             complement(1360..2328)
                     /locus_tag="PK345_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HPX34594.1"
                     /translation="MHYLSDVEAIDVFGKKIELLRKEIAKVIIGQDEVVKDVLISIFS
                     KGHVLLVGVPGLAKTLLVNTISKVLGLSYSRIQFTPDLMPSDIIGTEILDQSRNFKFI
                     KGPVFANIILADEINRTPPKTQSALLEAMQEKAITVAGKHYQLAEPFFVLATQNPIEQ
                     EGTYPLPEAQLDRFMFNIWLDYPSFEEEINVVKTTTTTYEGKPEVIISGEEILFFQDL
                     IRKIPVADNVYEYAVRLATKTRANRKNSHPWANEYLTWGAGPRASQYLIIGAKCHAAF
                     NGKYSPDIEDVKRVATSILRHRIVRNYKAEAEGITIEKIINEFLKD"
     gene            complement(2377..3726)
                     /locus_tag="PK345_05955"
     CDS             complement(2377..3726)
                     /locus_tag="PK345_05955"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPX34595.1"
                     /translation="MRKAILIIIPLLLFQLFEAAGQPLTGTVIDEVVAVVGKEYILFS
                     DIEQQYLQMKLQSQVHNSEEFTRCMILESMLFEKLLLHQAEIDSVETTPEQVESELDR
                     RMRYFISQFGTQEKMEQFYDKTVNDFKNDLREVITKQIKIEKVQQSITENAAVSPADV
                     RKYFKSIPRDSIPLIPSIVEISQIVKKPVITPEEKFEVKERLLGYRKRIINGESFEAL
                     ARLYSEDPGSARQGGDIGLRGRGELYPEFEAVAFKLEPDEISDIVESEAGYHIIQGIE
                     RRGDFVRVRHILLKPKVSPFEMEKARIYLDSVAQLIKHDSISYDDAVIKFSTDPSRNN
                     AGNLINPNTGSIQWSPEELDPKVFFVIDKLDVGVVSVPVIMQDEQGVEAYRLLYLRKR
                     TQPHRANLEEDYDQIQNWALNKAKGEMVQKWINNNARKAYIRIDEKYSNCDFQNHWR"
     gene            complement(3736..4584)
                     /locus_tag="PK345_05960"
     CDS             complement(3736..4584)
                     /locus_tag="PK345_05960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPX34596.1"
                     /translation="MKFPILTFFLFLLIFSSCINDQPVTDAKPVAKVYNNYLYESELL
                     GLVPFGASQEDSMAIISSYINNWIKKQLLLTQAEENLTKQQKDFSKQLEEYRNSLILY
                     TYESELVRQNLDTVVNMWEMERYYNENTENFVLGEDIVKFLYIKIKDDAPALKQIVKL
                     FKTNFVRNLDSLVYYAINFSEDYSLVTEDWVPFNRFLSKMPFNVDNSKYFLENNRFVE
                     VKEDSFHHLIYFIDYKLKGAQAPFEYELANIKLIILNKRKKLLIREMHQKIYEQARNR
                     NDFEYF"
     gene            complement(4593..6599)
                     /locus_tag="PK345_05965"
     CDS             complement(4593..6599)
                     /locus_tag="PK345_05965"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HPX34597.1"
                     /translation="MRSSLSTTLKLLTIGILLFSTSLFAQKETADPVLLSIDKQDITL
                     SEFLSVYKKNNLEGEVLDRKSLEEYLELYINFKLKVREAEELGMDTVQSFINELEGYR
                     DQLAKPYFVDEKLNEQLLKEAYERKKTDLRASHILITLDQYAPPKDTLEAYNKIMEIR
                     KRIVDNKENFNDVASEVSDDPSARDMPARGYQPARKGNKGDIGYFSVFDMVYPFEEMA
                     YNLKLNEVSKPLRTQFGYHLVKLTAKKPALGKVQVAHLYLQVPQDPTEENNAKLKIRV
                     DSIYNALKAGANWDTLVRKLSDDKSSSMNGGILPWFNSNRMVPQFIDGIRSIKDSGQI
                     SQPVLTSYGWHIIKLLDTKPIGTFEDEKTNLKQSLTKDSRADLSKEAVINKVKKEAGF
                     KKYDQAIIEFYPLMDSSIFIRKWETEKAAHLTKVMASLGDEEYNQQEFATYIAKNQSI
                     NPKETVSQFIDRLFDKFIDEKVIEYEDARLESKYPEFRDLIKEYRDGILLFELTDEKV
                     WSKAIKDTTGLKEFYEKHQNNYMWDKRANAILFVSNTKVGAEKALLMTQQRMSVDQIK
                     ESFVHDTVNTIKITEKKFSKWDNDIIDKIEWIEGTSPIIHEPEKDFSFAIIRSIVDPE
                     PKSLSEARGIITADYQNFLEQEWLKQLRKKYTVNVNQDVLSQIK"
     gene            complement(6717..8189)
                     /gene="guaB"
                     /locus_tag="PK345_05970"
     CDS             complement(6717..8189)
                     /gene="guaB"
                     /locus_tag="PK345_05970"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HPX34598.1"
                     /translation="MMQHQDKFVGEGFTYDDVLLLPAYSDILPREVDLSTFFSTGIRM
                     NIPIVSAAMDTVTEHKLAIAIAQEGGIGVLHKNMDIEKQGIEVKKVKRAENGMILDPI
                     TLMKDAKVEDALHIMREYSIGGIPVVDNDHKLVGIITNRDLRFERNLQRPVDEIMTKE
                     NLVTTTEFTDFEKAGDILQEHRIEKLPVVDKDFKLVGLITYKDIIKIKARPNACKDER
                     GRLRVAAAVGITSDTIDRVDVLVANQVDTIVVDTAHGHTRGVIETTKLIRRKYPDLQL
                     VVGNIGTAEAARDLVKIGVNAVKVGIGPGSICTTRIIAGVGIPQLSAIYKVSCAIKDS
                     GVPVIADGGIRYTGDIAKAIAAGAHSIMAGSLFAGVDESPGDTVIFEGRKYKTYRGMG
                     SIEAMQSGSKDRYFQDVEEDINKLVPEGIVGRVPYKGTLSEVIYQLVGGLRAGMGYTG
                     SHNISELQKARFIKITAAGVKESHPHDITITREAPNYSKI"
     gene            8671..9840
                     /locus_tag="PK345_05975"
     CDS             8671..9840
                     /locus_tag="PK345_05975"
                     /inference="COORDINATES: protein motif:HMM:NF015131.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HPX34599.1"
                     /translation="MQVIFNSGRFFVGIMLLTTMFFFLFATCSKNKTDQLFNPTPYEI
                     EIPYGFPTLLNIPVDNPMTVEGVELGRYLFYDGRLSGRTHPDSMMSCATCHIQNRAFE
                     CGADHHHFSGGFTFGLTGKYTHHVMLSLINLVWNSNGYLWNGGISKYNPDIHARNLED
                     IVWMAVIAEDEIAGDTTRTKQLISSITGYPDLFFKAFGSKEITMERISKAIAQFVRTL
                     VSANSKFDHYLRGEYQLTAEELRGFVLFTTEEGADCFHCHGGGGNPLFTTNLFYNNGK
                     DDEFDDLYDRYSITNNPVDIGAYKAPTLRNVELTGPYMHDGRFSSLEEVIEFYSHQVK
                     NSPYINPLMHHVANGGTQLTPSEKTDLMAFIKTLRDDDFLNNPNFSRPSKFPDEQ"
     gene            complement(9993..10063)
                     /locus_tag="PK345_05980"
     tRNA            complement(9993..10063)
                     /locus_tag="PK345_05980"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10029..10031),aa:Cys,seq:gca)
     gene            complement(10341..11051)
                     /locus_tag="PK345_05985"
     CDS             complement(10341..11051)
                     /locus_tag="PK345_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HPX34600.1"
                     /translation="MNNASQKVKVLLAEDNKNLGSVLKSYLNIKGYETTLCANGEEAL
                     ITFKKGYFGFCIIDVMMPIKDGFTLAKEIREIDKTIPILFLTAKSMQEDKLKGFSVGA
                     DDYLIKPFSMEELLARMTAILRRARERRGGEIGNNIFKIGEYIFDFNRQMLTYKDTDF
                     KLTSKESSLLRLLSLNLNHVLERSYALKEIWNDDSYFNARSMDVYITRLRKFLQQDEN
                     VELINVHGVGFKLISNIQ"
     gene            complement(11347..11421)
                     /locus_tag="PK345_05990"
     tRNA            complement(11347..11421)
                     /locus_tag="PK345_05990"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11384..11386),aa:Pro,seq:cgg)
     gene            11609..>12844
                     /locus_tag="PK345_05995"
     CDS             11609..>12844
                     /locus_tag="PK345_05995"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HPX34601.1"
                     /translation="MKKSYLHLVLFFFFFFSINAIAQHQNIKIVDTNSPNEPTIAIDP
                     NNTQRIIGGSNLNIYFYSNDGGYTWQNGILTSVNHGVWGDPVTLVDNEGNYYFFHLSN
                     PASGNWIDRIVCQKSTNGGVTWNDGTYMGLNGTKAQDKEWGTIDRNNGNIYVTWTQFD
                     DYGSSNPNHRTNIMFSKSTDSGATWSPAKRINEVDGDCIDSGNTVEGAVPTVGPNGEI
                     YVSWAGPLGIVFDKSYDEGETWLDNDIFVSDFPGGWAFDIPGIYRCNGMPVTVCDVSG
                     GPNHGTIYINWSDQRNGIDDTDVWLVKSTDGGETWSERKRVNDDPPGKQQFFTWMTVD
                     QITGDLHFVFYDRRNHNDNHTDVYGAISRDGGETFFNYKISEEPFLPSSWIFFGDYNN
                     ISAHDNVIRPIWTRMHNNQL"
BASE COUNT         3655 a         2494 c         2389 g         4306 t
ORIGIN      
        1 cgccaaatat tatcccgagt gcagtgagta ccgaacggaa tttattgtta aaaacagctt
       61 ctacagccga attaagattg tacaaataac gtttcacaaa ttatttgttt aatggcgtta
      121 attgcagatc agcgtggttt tcaggaaagt taagcagcac ttcatctccc tgtttcaacc
      181 cttcaaggat aattatctca ttgttattgg actggcctac tcttacctgt ttttttatcg
      241 tccgcatgcc tgtttcctga aacacgaagt taattgtatc attgctatga agcgcctcga
      301 tagggatata aagcacgcta tcaacctgtt cggtgatgat cacatttttt gtggtcatgg
      361 ctggtcgcat aatggtgtcg gtttcatcaa cacttatcac gacttcaaaa actttggcat
      421 cagaaccggg tctttgttcg ccaatattgg caacttcggt aacaataccg ttatattctc
      481 tatccggaaa agcatcaata gagattttaa caggttgccc tacccgaact tttgaaatat
      541 caatctcatt cacataggtt ttagtgatca tctttgaaag gtctggcaag agcgccacca
      601 cattttccca ttggtttatt gttgatcctg tctctgtttt ccttcctcgc caatcacgct
      661 tgtaaatcac cattccagat tgaggagcat atatggtaaa ttttttgagg atttcgagca
      721 tgttctggcg ttttcgtctc gcttgttcta atgttgcact aacctcttgc atttcagctt
      781 ttgatttttc taacctaagt aaataatttt ctactgcctg atcgtaagca cgattagctt
      841 tatcgaggtt gatctcttcc tgtcgctgta ctgcaggtgg ctcgaaaatg gattgttcga
      901 cgcgaatctg ttgctcttca aggccaaatt taagatttat cagatcgttg cgtgctgctc
      961 ttagtgtgag cgaagtgtcg agcattgtct ttgtgtactg cgattcgagc ttttcaagct
     1021 ctgtttcaat atctttgagc ttgctcatca cttcggtttg atcaatagag gcaacataat
     1081 cacctgaatc tacaagagtt ccctcaggga taagtttatt gattttaaga tcgccccata
     1141 tctcaagtga aagaagattt ccggggccaa atatattttc agaacttttg gctccaagtt
     1201 cacctgttgt gctcacatca acaatgaaca tcccgaactt aacaggtact ttaatatgtt
     1261 cgaccgactt ggtgtttttt ttagtaaaat accatactgc aaaaatgagc accaaagcag
     1321 atatagttaa tataagcagc tttttattca ttgttttttt taatctttca aaaattcatt
     1381 gataattttt tcgatggtta ttccttcggc ttccgctttg tagttacgta caattctatg
     1441 gcgaaggata gaggtggcaa ctctctttac atcttcaatg tctggtgagt atttaccatt
     1501 gaaagccgca tgacattttg caccaatgat aagatattgc gaagctcgtg gcccagctcc
     1561 ccatgttagg tactcgttag cccagggatg tgaatttttt ctattcgctc tcgttttggt
     1621 agccagcctg acggcatatt cataaacatt gtcagctacc ggtatttttc gtatcagatc
     1681 ctgaaaaaag agtatctctt caccactgat tattacttcc ggcttgcctt cgtatgttgt
     1741 agtagtagtt ttaacaacat ttatctcttc ttcgaaagat ggataatcga gccaaatatt
     1801 aaacatgaaa cggtcgagtt gtgcttcagg tagtgggtat gttccctctt gttcaatagg
     1861 attttgggtt gctaatacga aaaatggctc agcaagctga taatgctttc cggcgactgt
     1921 aatagctttt tcctgcatag cttccagcag cgctgattgt gttttgggtg gtgtgcggtt
     1981 tatctcatcg gcaagtataa tattggcaaa gacagggcct tttataaact taaaattacg
     2041 cgactggtct aaaatttcag tgccaatgat atcggacggc ataagatcag gggtaaactg
     2101 tatgcggctg tatgataacc caagcacctt tgatatggtg ttaaccaaaa gggttttagc
     2161 taagccgggg actccaacca aaagaacatg ccccttggag aatatggaga tgagcacatc
     2221 ttttacaacc tcatcttgcc ctataattac cttggcaatc tctttgcgga ggagctcaat
     2281 ttttttaccg aaaacatcaa tcgcttcaac atcagatagg taatgcataa aattcgcttt
     2341 ttaataatta gtaaagtatt tttttagggc tattgatcag cgccaatgat tttgaaaatc
     2401 gcagttgcta tatttttcat caattcggat ataggctttg cgggcattgt tatttatcca
     2461 tttttgtacc atctcacctt ttgctttgtt caatgcccag ttttgtattt ggtcgtaatc
     2521 ttcttcaaga ttagcgcggt gtggttgtgt tctttttctc agatagagca gccggtatgc
     2581 ttcgactccc tgttcatcct gcattattac tggtacggaa acaactccga catctaattt
     2641 gtcaataaca aagaacactt tagggtcgag ttcttccggc gaccactgaa tactgccggt
     2701 atttgggttg atgagattac ctgcgttatt acgtgatggg tcggttgaga attttataac
     2761 cgcatcgtca taacttattg aatcatgttt tattaattgg gcgacactgt cgaggtatat
     2821 tcttgccttt tccatctcaa atggcgacac cttgggtttt agcagtatat gccgaactct
     2881 cacgaaatcg cctcgccgtt cgattccttg aatgatatga taaccggctt ctgattctac
     2941 aatatccgat atttcatcag gttcgagttt aaaagcaacg gcttcaaatt cggggtaaag
     3001 ttcgccgcgt cccctcaggc cgatatcacc tccttgtcga gctgagccag ggtcttcgga
     3061 ataaagtctg gctaatgcct caaaactctc accatttata attcttttgc gataacctaa
     3121 aagccgctct ttgacttcaa atttttcctc aggcgtaatc acaggctttt ttacgatttg
     3181 cgaaatttca actattgaag gtatgagggg gatgctgtcg cgtggtatgc ttttgaagta
     3241 ttttcgaacg tctgctggcg acacagcagc attttcagtt atggattgct gtaccttctc
     3301 tatttttatc tgtttggtaa ttacctcccg aaggtcgttt ttgaaatcat taaccgtttt
     3361 gtcataaaac tgctccattt tctcttgtgt gccaaattga ctgataaaat aacgcatcct
     3421 cctgtcaagc tccgattcaa cttgctcggg agtggtttct acgctgtcaa tttcagcttg
     3481 atgcaacaac agtttttcga aaagcatgct ttccaatatc atacatctgg taaactcttc
     3541 gctgttatgt acctggcttt gtagtttcat ttgcagatac tgctgttcaa tatccgaaaa
     3601 cagtatgtac tcttttccta cgacagccac tacttcatca ataaccgtcc cggtaagcgg
     3661 ctgcccggca gcttcgaata actgaaacaa caacagaggg ataattatta aaatggcttt
     3721 tctcatagat ggattttaaa aatattcgaa gtcatttcta tttcgtgcct gttcatatat
     3781 cttttgatgc atctctcgga taagtagttt cttgcgttta ttaagtatta tcagcttgat
     3841 gttggctaac tcatattcaa acggagcttg agcgcctttt aatttgtaat cgatgaaata
     3901 tataagatga tgaaaggagt cctctttgac ttcaacaaaa cggttgtttt caagaaaata
     3961 tttagagtta tcaacgttaa acggcatttt cgataaaaaa cgattgaaag gcacccaatc
     4021 ttcagtaacc aatgaatagt cttcgctgaa attgatggca taataaacca atgagtctaa
     4081 gttacgaaca aaatttgttt taaaaagttt aactatttgt tttaaagccg gcgcatcgtc
     4141 tttgatcttg atataaagaa acttaacaat atcctcgccc agaacaaagt tttcagtatt
     4201 ttcattgtaa tatctttcca tttcccacat attaactact gtgtccaaat tttgacgcac
     4261 cagctcactc tcataggtgt ataggattaa cgaattgcga tactcttcca gttgtttgga
     4321 aaaatctttt tgttgtttgg tcagattctc ctcagcctgc gtaagcaaaa gttgtttttt
     4381 gatccaatta ttgatgtagc tgctgatgat agccatgctg tcctcttgtg aagcgccaaa
     4441 aggaaccagt cctaagagtt cagattcata gagataatta ttataaactt tggcaacagg
     4501 ctttgcgtca gttaccggtt gatcattaat gcatgaactg aaaataagta ggaaaagaaa
     4561 aaaagtaaga ataggaaatt tcaagaccag cgctatttta tttgcgaaag aacgtcttga
     4621 ttaacgttta cagtatattt ttttcgcaat tgttttagcc attcctgttc gagaaagttt
     4681 tgatagtcgg ctgttataat acctctggcc tcactcaggc ttttaggttc gggatcaacg
     4741 atggatctaa ttatagcaaa actaaaatcc ttttccggct catgaattat tggagaagtc
     4801 ccctctatcc attctatttt atcaattata tcattatccc attttgaaaa ttttttctct
     4861 gtaatcttaa tagtgttgac ggtatcatgg acaaaagact ctttgatctg atcaacgctc
     4921 atacgttgtt gtgtcatcaa taatgctttt tcggctccta ctttagtatt ggaaacgaac
     4981 aaaatagcat tggcacgctt atcccacata taattatttt gatgtttttc gtaaaactct
     5041 tttaggcctg tggtgtcttt aatagctttg ctccatacct tttcgtcggt aagttcaaaa
     5101 agtaaaatac catcgcgata ctcttttatt aaatctctga actcaggata tttactttcg
     5161 aggcgggcat cttcatattc aatcactttt tcatcaataa atttatcgaa taggcggtca
     5221 ataaattgac taacagtctc ctttgggttg attgattgat ttttagcaat ataggttgca
     5281 aattcctgct ggttatattc ttcatctcct aagctagcca ttactttagt cagatgagct
     5341 gctttttcag tttcccattt gcggataaat atacttgaat ccatcagagg atagaactct
     5401 attattgctt gatcatattt tttaaaaccg gcctcttttt ttactttatt tataactgcc
     5461 tctttgctta ggtcggcacg cgaatctttt gtaagacttt gcttaaggtt tgtcttttcg
     5521 tcttcaaatg tgccgattgg ctttgtgtca agcaatttga tgatatgcca gccgtaggag
     5581 gtgagtactg gttgagaaat ctggccggaa tccttaattg atcttatacc gtcaataaac
     5641 tgtggtacca tgcggttcga gttaaaccat ggcaatatgc cgccgttcat tgatgagctt
     5701 ttatcatccg acagcttacg aactaatgtg tcccaattgg ctccggcttt tagggcattg
     5761 taaattgaat caacccttat tttgagctta gcattgtttt cttcagtagg atcttgagga
     5821 acttgcaagt agagatgagc tacctgtact ttgccaagtg cgggcttttt tgcggtcagc
     5881 ttaactaaat gatatccaaa ttgtgttcgt agtggttttg aaacttcgtt caatttaagg
     5941 ttatatgcca tttcctcaaa gggatatacc atatcaaaaa ctgagaaata gccaatatct
     6001 cctttatttc ccttacgtgc aggctgatac ccgcgggctg gcatgtccct tgccgatgga
     6061 tcatcagaaa cctcagaagc cacatcatta aaattttctt tgttgtcaac aattcttttt
     6121 cttatctcca ttatcttgtt gtaggcttca agtgtgtctt tcggtggagc atactggtca
     6181 agcgttatca agatatgact agctctaagg tctgtttttt tacgttcata ggcctcttta
     6241 agtagttgct cattgagttt ctcgtccaca aaatagggtt tagcaagctg atcacgatat
     6301 ccttccaact cattaataaa cgactgaacg gtatccatac caagctcctc tgcctccctt
     6361 actttaagtt tgaaattgat gtaaagttca agatactcct ccaatgattt tctatcgaga
     6421 acctcacctt caaggttgtt tttcttatat accgacaaaa attcagataa ggtaatatct
     6481 tgcttatcaa tactgagtaa tacggggtcg gcagtctcct tttgagcaaa aagtgatgtt
     6541 gaaaacagca aaatgccgat agtcaataat tttagtgtag ttgataatga acttctcata
     6601 actttgtttt tatttttatt tttaaaatat ttattgcaga atttttaaac agttagtatc
     6661 agattttcaa atacgataaa ttatgttgat tattatattt ttgatacact atgtacttaa
     6721 attttgctgt aatttggggc ttcacgtgta atagtaatat catgcggatg gctttcttta
     6781 acacctgctg cagttatttt gataaacctt gctttttgta attcgctgat attatgcgat
     6841 ccggtatatc ccattccagc gcgtaatcca ccaacaagct gatatattac ctcgctcaat
     6901 gtacctttgt agggcactct tcctactatt ccttcaggta caagtttatt gatgtcctct
     6961 tctacatcct gaaaatagcg atctttggat cctgattgca tagcctctat tgatcccata
     7021 cctcgatagg ttttatactt cctaccttca aatattactg tatcgcctgg tgattcatct
     7081 actccggcaa ataacgatcc ggccatgata gagtgtgctc cggcagcaat ggctttggca
     7141 atatctccgg tataacgaat tcctccatca gctatcaccg ggacacctga atccttaata
     7201 gcgcatgaaa ccttgtaaat agcgctcaat tgtggtatgc ctacaccggc aataattcgt
     7261 gtagtacaaa tagagccagg gccaatacct acttttactg cgttaacacc aatctttact
     7321 aaatcgcggg cggcttcagc agtgccgata tttcctacta ctaattgtaa atcaggatat
     7381 ttacggcgta ttagctttgt ggtttcaata acgccgcgtg tatggccatg ggcggtatct
     7441 actacaatag tatctacttg gtttgcaacc aatacatcca ccctgtctat tgtatcgctg
     7501 gttattccta cggcagcggc aacacgcagg cggccgcgtt catccttgca agcattcgga
     7561 cgtgccttta tcttaataat atccttgtag gtaataagcc ctactaactt gaaatcctta
     7621 tccaccaccg gcagtttttc aattcgatgc tcctgaagta tgtcaccggc tttttcgaaa
     7681 tctgtaaact cagttgttgt tacaagattt tctttagtca ttatttcatc aacaggcctt
     7741 tgcagattac gttcaaaacg taaatcgcga ttagtgatta tccctactaa tttatgatcg
     7801 ttatctacaa ccggaatccc accaatacta tactccctca taatatgcaa agcatcctca
     7861 actttggcgt ctttcataag ggtaataggg tcgagaatca taccattttc ggctcgtttt
     7921 acctttttta cttcaattcc ctgcttttca atatccatat ttttatgtaa aactccaata
     7981 cccccctcct gcgcaatagc tatagccagt ttgtgttcgg taactgtatc cattgcagca
     8041 gaaacaatag ggatgttcat acgaatacct gtggaaaaaa aggtagatag atcaacttcg
     8101 cgaggaagaa tgtcgctgta agcgggtaat aaaaggacat catcgtaagt aaaaccttcg
     8161 ccaacgaatt tatcttgatg ctgcatcatg atgaaattta gctaaaattt gcacaaaggt
     8221 acgttttttc caaaagttag atatcaataa atacgatata ctgtttgaaa gataaacact
     8281 aggagtgagc gtttgatcag cttttaaaaa ttattctgcc cgaaagagct ttgtgttgat
     8341 taattcaggt taaaacttct tattatattt ttggataata caacgtttag ggacttggtg
     8401 atgaccggga attctaatac tgatgcttat cctcggaaca aaagttgaat ttagcatcct
     8461 gatagctgtc aggattgata cgatgccgct cagatccgcc tgtataataa cctttaaaaa
     8521 ttttcattac actttaaaaa cataaactta cgctgatttt ataccttaat aaaatttttc
     8581 tttagcaaaa tattgcaata atcttaaata ttaaacgctt tcaaaacata ataaaccttt
     8641 ttactactaa tgaagcggct aaaaataata atgcaagtta tttttaattc aggcagattc
     8701 tttgtcggta ttatgcttct tacaacaatg ttttttttct tgtttgcaac ttgctccaaa
     8761 aataaaaccg atcagctatt caacccaact ccctatgaaa ttgaaatccc ctatggattc
     8821 cctacgctac taaatatacc tgttgataat cctatgacag ttgagggggt ggaattgggt
     8881 cgttatcttt tttatgatgg tcgtctttcg gggcggacgc atccagactc gatgatgagt
     8941 tgcgctactt gccatattca gaatagagcc tttgagtgtg gcgctgatca tcaccatttc
     9001 agtgggggat ttacgtttgg actaacagga aaatatactc atcacgttat gttatccctg
     9061 ataaacttag tgtggaatag taatggatac ctttggaatg gaggcatatc aaaatataat
     9121 ccagatatac atgcacgtaa ccttgaggat atagtctgga tggctgtgat agcagaagat
     9181 gaaattgcag gagatactac gaggacaaaa caattgatca gcagtattac cggttatcct
     9241 gatctttttt ttaaagcttt tggaagtaag gaaattacaa tggaacgtat ttcgaaggca
     9301 attgctcaat ttgtacgcac attagtttcg gccaattcca aatttgacca ttatctgcgt
     9361 ggtgaatacc aactgacagc ggaggagttg cgtgggtttg tactgtttac gactgaggag
     9421 ggagccgact gcttccattg tcatggtggc ggaggtaatc ctcttttcac aaccaatctt
     9481 ttctataata atggtaaaga tgatgaattt gatgatcttt atgatcgtta tagcattacc
     9541 aataatcctg ttgacatagg cgcttacaaa gctccaactc tacgtaacgt tgaactgaca
     9601 gggccgtata tgcacgacgg gcgtttttct tcattagaag aggttattga gttttattcc
     9661 catcaagtaa aaaactcccc ttacatcaat cccttgatgc accatgttgc taatggtgga
     9721 acacagctta caccatctga aaagaccgac ctgatggctt ttataaaaac acttagagac
     9781 gatgattttt taaataatcc taatttctca agaccctcaa aatttcctga cgaacaatag
     9841 aatatgataa tgattatcca aattcattgt cgctaattct atagttttat cccgaattta
     9901 tcaatagata agtacttcta atctagaatt attgtaacat attgaaagaa aaaacccgat
     9961 gaagtggtaa tcttcatcgg gttttaaagt agaggtacca agcggagtcg aaccgctgta
    10021 cacggttttg cagaccgttg cctaaccact cggccatggt accgtatcaa aatataaata
    10081 tgggcggcaa atataaccat tttgtcctca cagaaacatt tttttaaatt tttcatacat
    10141 tacattgaaa aggatcataa acttttaaaa aggttgtaac aaatcctaaa aactaaaact
    10201 aacagatgtg tagattaaaa atgtcgccgt tttgttagtc aagttatctg ctgtgaacat
    10261 ctttaatgcc aatactaata ggcatttgtt taaaacaata ttatactatc aggaaataaa
    10321 agtgagcgtg atgaaattaa ttactgaata ttggaaataa gtttaaaccc aaccccgtga
    10381 acattaatta gctcaacatt ttcatcttgc tgtaggaatt ttctgagtct tgtaatatac
    10441 acatccatac tgcgcgcatt gaaataacta tcgtcgttcc agatttcttt taaagcataa
    10501 cttctttcaa gtacatggtt aagatttagt gaaagcagtc gcagtaaaga agactctttc
    10561 gatgtcagct taaaatctgt atccttgtag gttagcatct gtctattaaa atcaaaaata
    10621 tattctccga ttttaaaaat attatttcct atttctccgc ctctccgttc ccttgcacgt
    10681 cgcaagatag cagtcattct agctaaaagt tcttccatgc taaagggttt gattaggtag
    10741 tcgtcagctc ctacagaaaa tcctttaagc ttgtcttctt gcatagattt ggccgttaaa
    10801 aaaagaattg ggattgtttt atctatctct ctaatttcct ttgctaatgt aaatccatct
    10861 tttataggca tcattacgtc aatgatgcag aatccgaaat atcctttttt gaaggtaatt
    10921 aatgcctcct ctccatttgc gcaaagggtt gtttcatatc cttttatatt taaatatgat
    10981 tttaaaacgc taccaaggtt tttgttgtct tcagctaata gcacctttac tttttgtgat
    11041 gcattgttca tgaaaatcaa agttttggat taatatttca ggtggcaaaa gtaactgctt
    11101 tttcctaatt gaaaataaac tgcttaatta tttgtttttt gattttccgt ggaatggtag
    11161 aaaaatggtg aatttagagc cttttgttgg cttactctca acagtttaaa tacacaccat
    11221 gttctatcta caattgttat tacaaaactt aaccttaagc taactccctt tacattatgg
    11281 attacgaata gaatattgcc taaaaaaaaa gctgtttcaa aaacaatgaa acagcctcta
    11341 ttactgtcgg gatggcgaga ttcgaactcg cggcctccac gtcccgaacg tggcgcgcta
    11401 gccgggctgc gctacatccc gcaaagaatt atgtggcaaa attagaaaat attacttacc
    11461 tatccggcta aaaagcattt tataacaaca tttttttgtg attttattac tcaatatctt
    11521 tataaattta atgttttaag agttggttat caaaagacag atattttaat agtattattt
    11581 ttgctgttta acattaatca tattttttat gaaaaaaagt tatttacatc tggtcctatt
    11641 cttcttcttc ttctttagca ttaatgctat tgctcaacac caaaacatca aaatagttga
    11701 tactaattct cctaatgaac cgaccattgc tattgatcct aacaatactc aacgtattat
    11761 tggggggtcg aatttgaata tttattttta tagcaacgat ggaggatata cttggcaaaa
    11821 tggcatattg acatcggtta accatggcgt gtggggcgac ccggtaactt tggtggataa
    11881 cgaagggaat tattattttt tccatttatc taatccagcg tcgggaaact ggattgatcg
    11941 tatcgtttgc cagaaatcaa ctaacggcgg agtaacttgg aacgatggta catacatggg
    12001 gcttaatgga acaaaagcac aagataaaga gtggggtact attgatcgaa acaatgggaa
    12061 tatttacgta acctggacgc agtttgacga ttatggaagt tccaacccta atcatcgtac
    12121 taacataatg ttttctaaat caacggatag cggtgcgaca tggtcgccgg caaaaagaat
    12181 taacgaagtt gatggtgatt gtattgacag tggcaatacc gtagagggtg ccgtgccaac
    12241 tgtagggcct aacggagaga tttatgtatc ttgggcaggt ccgcttggta ttgttttcga
    12301 taaatcgtat gatgagggtg agacatggtt ggataatgac atctttgtat ctgattttcc
    12361 gggaggttgg gcatttgaca ttcctggaat ataccgttgt aatggaatgc cggttactgt
    12421 gtgcgatgta agtggcggcc caaatcacgg aacgatctat attaattggt cggatcagcg
    12481 taatggcata gacgacaccg acgtttggct tgtaaaatca accgatgggg gtgaaacatg
    12541 gtctgagaga aaacgtgtaa atgatgatcc gcccggcaaa caacaatttt ttacatggat
    12601 gactgtagat caaataaccg gcgatctgca ttttgtgttt tacgaccgtc gaaatcataa
    12661 cgataatcat actgatgttt atggcgcaat atcgagagac ggcggagaga cattttttaa
    12721 ttacaagatt agtgaagagc catttctacc cagctcatgg atattttttg gtgactataa
    12781 taatatttct gctcatgata atgtaatcag accaatttgg acaaggatgc ataataatca
    12841 atta
//
LOCUS       DAOOAI010000067        12660 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_6395_length_12660_cov_5.517493, whole genome shotgun sequence.
ACCESSION   DAOOAI010000067 DAOOAI010000000
VERSION     DAOOAI010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12660)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12660)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12660
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6395_length_12660_cov_5.517493"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            76..2010
                     /locus_tag="PK345_06000"
     CDS             76..2010
                     /locus_tag="PK345_06000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3857 and transglutaminase domain-containing
                     protein"
                     /protein_id="HPX34602.1"
                     /translation="MLVLIFCGFIINADPVEDLIKTSGDADKYKGSNVLVLFDSTRVD
                     VQQTGLSHFHIHQLFKVLTQAGALELRVIKVDYDPISADAEIRLLRVFRRNGDVEILD
                     LKNVKDYAAPARGIYWGAREKMIEVGRLEPGDVVELKYFKKGFTYALLMDDDDDRFIP
                     PMRGHYYDIVPFWSNHPMLEKVYIAQLPADKPLQYQFYNGECQSSLRFENGKHIYTFS
                     MKNFMPLKREPNMLDFFDVAPKLILTTSPDWEAKSRWFYNVNEDYGSFEPTPEAEAFV
                     KELLKDAKTELDSVSILTHWVGDNMRYSGISMGSNEGYTLHNTEMNFRDRCGVCKDKA
                     ALLISFLRIAGFETYAAMTMAGSRIEDIPADHFNHSVTVVKLSDGKFHLLDPTWVPFI
                     RELWSSAEQQQNYLIGLPEGADLMITPVSPPENHYLKIKARSSIMADGTLEGSLSVEA
                     EGQTDAAVRGIFIREYKSEWDKALEAELLKVHPAAVITQMDYGDPIDYLSGPITINIS
                     YQIKGYAITGDNEIIFTPLLATPFMSRAMGHLYFNTDQEEKKYGFRDRCSRLVELEET
                     ITFPDNFKPLLPDVNKTTSGNSARFSGGYRIKGNSIQLNQEIVLGKRIYEAADWNEFR
                     EAVKGQKKISEMPVVLKKSN"
     gene            2041..3861
                     /locus_tag="PK345_06005"
     CDS             2041..3861
                     /locus_tag="PK345_06005"
                     /inference="COORDINATES: protein motif:HMM:NF024371.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3858 domain-containing protein"
                     /protein_id="HPX34603.1"
                     /translation="MKKNCLIILSLFFAHFLFSQNENADAIFEKFIKEFTLEADGSTS
                     YREYKQLKLITQFSFNRLYGETFIIYNPEFQNLKINDAYTVMADGSKVITPPNAFNEV
                     LPRDAAFSATANHMKEMVVTHTATEMGATIFLDYTLTSAKGYLPGFMGNVLIQESSPI
                     AELEVIVRIPSDQELNHRMIGLRTGPEIKIEANQKVYVWKFNGVQASPKEPLQGKYQL
                     SAPRLLFTTVIGVAETINWLTNQQAFELQINDEMKAWTDNLRSKQTEEIETLMTIQKA
                     VVENIVFERYNPEWLAYRARTPEEIWKSNGGNKLEKTLLLATLLKYANFNATPAIVGP
                     KSLFDEKAINLTIFDDFVLIVNTKSFGTIYMSAVEISNQSLEYSLAQNIFIPLSKGIS
                     RAPVEPVKMDNKINIKGTISFNEELKPSGTMEVELTGANNPFLLLQNDKGKFKSLISN
                     KMIKDDDAISIKNSNVAKSELLVKVESTESISDNNGYYSWQLPVITGGFEKWRIAYLD
                     KTRQSNLFLPYPIVEKYSYTITLPLDYTLINKRFNERLKNNVGTVSVEIKPKGNQIEI
                     NRELKLNTQMIYQIDYNEFRDMINFWLDKNLSTVVFKKKD"
     assembly_gap    4018..4027
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            4316..4816
                     /locus_tag="PK345_06010"
     CDS             4316..4816
                     /locus_tag="PK345_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018359053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPX34604.1"
                     /translation="MSTMEFNSQLASLQDNLTYFAQSLTQNADDARDLVQETYLKALT
                     YRDKFEANTNLKAWTYTIMKNTFINNYRRNVKMSTKFDNTNDLIYLNSGSASEEATPD
                     SILSHKEISEVVSKLEDEHRIPFQMHHQGYKYKEIAEHLDLSIGTVKSRIFFSRKKLM
                     KALKEN"
     gene            5051..6352
                     /gene="metK"
                     /locus_tag="PK345_06015"
     CDS             5051..6352
                     /gene="metK"
                     /locus_tag="PK345_06015"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004372899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HPX34605.1"
                     /translation="MSYLFTSESVSEGHPDKVADQISDALLDEFLKLDPESKVACETL
                     VTTGLVVCSGEVKTSGWVDPQEVVRRVVNQIGYNKASYRFDGNTCGIISTIHEQSDNI
                     NKGVVKQVAEEQGAGDQGMMFGYAVREMDNLMPISIELAHIILQQLAAIRHEGKVMTY
                     LGPDSKSQVTIEYDDDNHPIRIDTIVVSTLHDEFDTDEAMLRQITDDVKKYLLPRVIS
                     ETPEKYRHLFKDDYKLFVNPSGKFVIGGPNGDTGLTGRKIIVDTYGGKVAHGGGAFSG
                     KDPSKVDRSGAYAARHLAKNLVAAGVADEALIQVAYAIGIAEPVGLFVNTFGTAKVKD
                     KNGNKLSDSKIAKIIKANYKLTPFEIIKRFELKSPIYLKTATYGHFGRDCYHKEVEVF
                     YEDDSTFVKVINNKKKIFKKVKYFGWECLDYVDVFKKAFNI"
     gene            complement(6440..6964)
                     /locus_tag="PK345_06020"
     CDS             complement(6440..6964)
                     /locus_tag="PK345_06020"
                     /inference="COORDINATES: protein motif:HMM:NF017538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqiA/YcfP family alpha/beta fold hydrolase"
                     /protein_id="HPX34606.1"
                     /translation="MSKIKTLYLHGLDSLPVSQKNEMMEKVGFEVFAPKIDYRIEKNI
                     YQNLRQKIIERKIEFLIGSSLGGYISFWLGEDLGLPCLLYNPAMTYSNQLDAYIPEIA
                     LGKCPARFIVIGNRDEVVDPNENLNFFRQLNIDNCYQRIIVCEWLAHQIDFQSFEELI
                     AWAATSYRVFKKSL"
     gene            complement(7031..9634)
                     /locus_tag="PK345_06025"
     CDS             complement(7031..9634)
                     /locus_tag="PK345_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HPX34607.1"
                     /translation="MRFWFITFLVIPLFLSAQMYDDFSDGDFTADPPWYGDVDDFIVN
                     SSFQLQLNAATAGISHLSTHYEMNGETEWQFWIKMNFAPSDNNMARVYLASDKQSLNG
                     PLNGYFLRFGENLSNDAIELYRQNGETLTLICRGTDGLIANAFQLWLRIRRDVYGNWT
                     IEKDNTGYGVYQQEATGFDDQILTSSFLGVYCKYTVSNIKNFCFDQFYAGPHIIDNEP
                     PQLVNIDATSANNLDIYFNEALLPAQAINTQNYFVSHSIENPMIAQLDTQNPGKVMLL
                     FDRSFPNGMPLTITIKNIADLNGNTAPTIESEFMWFRASAFDVQINEIMADPDPPVGL
                     PNVEFIEILNTTDKEINISGWRLIIGNTIKVFSQLKLQPNQYLLVGHENSAQELSQYA
                     LFYGFSGFQLPNSGQTITLKNMLDQVISTVTYSDEWYDTNKKDGGWSLEQIDPENPCG
                     GKNNWTASISSTGGTPGAPNSVKANNPDILAPQTARVEYISTTEIVLHFSEPMDSLLL
                     NVPQSYHISPSIGYPTQAIVKEPDYSRVAIILNPENPLKEDVVYALEVLNDLFDCAGN
                     LIDKSKITYFGIPRPVNPNDIVINEILFNPREDIVKGVDFVEIFNISDKILDLSTLVL
                     ATEDKSTGEISTPKNISKNGLLLFPENYMTLTTNPDVVKQQYYTENPDNFTKMESLPQ
                     YSNADGIVILATKGHEVIDRVEYSENMHIPLLKSYKGVSLERINPLRPSNDITNWHSA
                     AEDFGFATPGYKNSQYSDVTFIDDPITIQPDIFSPDMDGKDDVLNINYMFESPGFIAS
                     ITIFDTRGRLIRKLIQNELLGTEGTFSWNGITDHGQKAAIGIYIILFEAFDLNGNKRN
                     YKKTAVLGGYL"
     gene            9850..9921
                     /locus_tag="PK345_06030"
     tRNA            9850..9921
                     /locus_tag="PK345_06030"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:9883..9885,aa:Arg,seq:ccg)
     gene            10187..10561
                     /gene="gcvH"
                     /locus_tag="PK345_06035"
     CDS             10187..10561
                     /gene="gcvH"
                     /locus_tag="PK345_06035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HPX34608.1"
                     /translation="MNIPDNLKYTKEHEWIRMDGDFAIVGITDYAQNELGDIVYVEVE
                     TVGETLEKEETYGTIEAVKTVSDLFMPVTGEIIEFNEELEDNPEIINSDPYGKGWIIK
                     IKPDNPSDIMDLLDSAGYKDLI"
     gene            10660..11346
                     /locus_tag="PK345_06040"
     CDS             10660..11346
                     /locus_tag="PK345_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPX34609.1"
                     /translation="MDAKKTDRADLENKKNIFLQIGLVIALLFVLLAFNYKSYERATG
                     FDIQVQVDNAPEEIIPITKQEAKPPPPSPPRQITLINIVEDNVEVEDAIEIDVEATQM
                     TEIADFIPPVVTKGEEVVEELEIFTVVESMPEYIGGDEARMSFLQENIKYPQLARESG
                     IQGTVFVTFVVETNGSVTDVRVLRGIGGGCDEEAVRVVKIMPKWKPGMQRSKPVRVQF
                     TMPIRFTLQG"
     gene            complement(11565..12017)
                     /locus_tag="PK345_06045"
     CDS             complement(11565..12017)
                     /locus_tag="PK345_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013065575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HPX34610.1"
                     /translation="MQYVYDYPRASVTVDIIVFCMFDKEMSVLLIQRGKEPFREKWAL
                     PGGFIEMEETLLETGRRELKEETGLENINLHQFKSYGDPHRDPRGRTISVVFYGFTTP
                     ENSSVIGGDDASGAAWFPINRLPELAFDHNQILNDMISSNYFQSEINQ"
     gene            complement(12153..>12660)
                     /locus_tag="PK345_06050"
     CDS             complement(12153..>12660)
                     /locus_tag="PK345_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008145941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rRNA small subunit methyltransferase 1"
                     /protein_id="HPX34611.1"
                     /translation="HQNNEHQLVNKLCSQIAEGKKAALVSDAGMPAISDPGFLLIREC
                     LKNAIPVETLPGPTALLPALINSGLPADKFFFEGFLPTKKGRTTRLKFLSELPFTVII
                     YESPHRLLKTLEQLAGYFSKERKASVSRELTKIYEETIRGTLQEIILHFEQSAIKGEF
                     VIVVDGLK"
BASE COUNT         4102 a         2130 c         2456 g         3962 t
ORIGIN      
        1 aaattgcgat gattaaagtt gatgactttt taataaacaa taaatatttt atataataat
       61 aaaaaattgt ttttaatgct cgtgttaatc ttctgtggtt ttattattaa tgccgatcct
      121 gttgaagatt tgattaaaac atcaggggat gctgataaat acaagggaag taatgtatta
      181 gttcttttcg atagtacacg ggtagatgta caacagactg gccttagtca ttttcatatt
      241 catcaattat ttaaagtact aacgcaggca ggagcattgg agttgagagt aataaaagta
      301 gattatgatc cgatctcggc tgatgcagag attaggctgt taagagtttt tcgcagaaat
      361 ggtgatgtcg aaatccttga tttaaaaaat gtaaaagatt atgccgcacc tgctcgggga
      421 atttactggg gtgcacgcga aaaaatgatt gaagttggaa ggcttgagcc aggtgatgta
      481 gtcgaattga aatactttaa aaaaggtttc acctatgcac tgttgatgga tgacgatgat
      541 gatcgtttta tcccaccaat gcgcggtcat tattatgaca ttgtgccttt ttggagcaac
      601 cacccgatgc tcgaaaaagt atatatagca caacttccgg cagataaacc cttacagtat
      661 cagttctata acggagagtg tcaatcatca ttgaggtttg aaaatggaaa acatatatat
      721 acattttcca tgaaaaattt catgccattg aaacgagaac caaatatgtt agattttttt
      781 gacgtagctc caaaacttat tcttaccaca agccctgact gggaagcaaa atcaagatgg
      841 ttttacaacg taaatgaaga ttatggcagc tttgaaccaa cccctgaagc tgaagcattc
      901 gtaaaagaat tacttaaaga tgccaaaaca gagttagata gcgtctctat tttaacgcat
      961 tgggttggcg ataatatgcg atactccggt atctcgatgg gttcaaatga gggatacacc
     1021 cttcataata ccgaaatgaa ctttcgcgat cgttgtggtg tttgcaaaga taaggcggct
     1081 ttactcatct cctttttacg gattgccgga tttgaaactt atgctgcaat gacaatggct
     1141 ggctcgcgta tcgaagatat acctgccgat cattttaacc acagcgttac ggttgttaag
     1201 ctctccgatg gcaaattcca tctgcttgac cccacctggg ttccttttat tcgagaactt
     1261 tggtcaagcg ctgaacaaca acaaaactac cttatcggat tgcccgaagg cgctgatctt
     1321 atgataactc ctgtatcacc acctgaaaat cattatctga aaataaaagc cagatcaagc
     1381 attatggctg acggaacgct tgaaggaagt ctttctgttg aagctgaagg acaaacagac
     1441 gcagcagtga ggggtatatt tatccgtgag tataaatcag aatgggataa agcattagaa
     1501 gccgaattgt tgaaagttca ccctgctgcc gtgattactc agatggacta tggtgatccg
     1561 atagattacc tttccggccc aattactatt aacatttcat atcaaattaa aggttatgcc
     1621 attaccggtg ataatgagat aatatttacc cctttactgg ctacaccatt catgagtcgt
     1681 gcaatgggac atctttattt taataccgat caggaagaga aaaaatatgg ttttcgcgat
     1741 cgttgcagcc ggttagttga gttggaagag acaataactt ttcctgataa ttttaaaccg
     1801 ttactccccg atgtaaataa aactacatca ggaaatagtg ctcgtttttc ggggggttac
     1861 cgaataaaag ggaattcaat tcagttgaat caagagatag tattaggaaa gagaatatat
     1921 gaagctgctg attggaacga atttcgcgaa gcggtgaagg gtcagaaaaa aatatctgaa
     1981 atgcctgttg tactgaaaaa atcaaattag ttaactacaa aatattcaaa accgataatg
     2041 atgaaaaaaa attgtctgat tatattaagt ctcttttttg cccatttctt gttttcgcaa
     2101 aatgaaaacg cagatgcgat ttttgaaaag tttataaaag aatttactct cgaagctgat
     2161 ggaagcacga gttaccggga atataagcaa ttaaagctta ttactcaatt ctcctttaac
     2221 agactgtatg gcgaaacatt tataatttac aatccggaat ttcaaaattt gaaaattaac
     2281 gatgcatata cagtaatggc cgatgggtca aaagtaataa cgccgcctaa tgcttttaat
     2341 gaagttttgc cgcgagatgc tgccttttca gccacagcta atcacatgaa agagatggta
     2401 gttacacata ctgctaccga aatgggagct acaatatttc ttgattatac gcttacctca
     2461 gcaaaaggtt acctgcctgg ttttatgggt aatgtcctta tacaggagtc atcgcctatt
     2521 gctgaactgg aggttattgt ccgcatccct tccgatcagg aacttaatca tcggatgatc
     2581 gggctacgaa caggaccgga aataaagatt gaggctaatc aaaaagtata tgtgtggaaa
     2641 tttaatgggg tgcaagcctc tccaaaggaa ccccttcagg gaaaatatca actttcagcg
     2701 ccgagattac tttttacaac agttataggg gtagccgaaa caataaattg gctgaccaac
     2761 cagcaggctt tcgaactgca aataaatgat gaaatgaaag cctggacaga taatcttaga
     2821 agtaagcaga ctgaagaaat tgaaacattg atgacaatac aaaaagctgt agttgaaaat
     2881 atcgttttcg aaagatacaa tccggaatgg ttagcctatc gagcaagaac acctgaagag
     2941 atttggaaaa gcaatggagg aaacaaattg gaaaaaacat tgttgcttgc tacattactt
     3001 aaatacgcca actttaacgc tacacctgca attgttggtc cgaaatcatt atttgacgaa
     3061 aaagctatta atcttaccat ttttgacgat tttgtattaa tagtgaatac caaaagtttt
     3121 ggtacaattt acatgtcggc agtggaaata agcaatcagt cgcttgaata tagcctggca
     3181 caaaatattt tcattcccct ttctaaaggt atctcacgtg cgccggtcga acctgtgaaa
     3241 atggataata aaataaatat taaaggaaca atttctttta atgaagaatt aaaaccatcg
     3301 gggacaatgg aggtagaatt gactggcgca aacaatccct tccttttatt gcaaaatgat
     3361 aaaggaaagt ttaaatcgtt gatctcgaac aaaatgataa aagacgatga tgcaatttct
     3421 attaaaaaca gcaatgtagc aaaatcggaa cttttggtca aagttgagtc aacagaatca
     3481 atatctgata ataatggtta ctatagttgg cagctgccgg taataaccgg cggttttgaa
     3541 aaatggcgaa tagcttattt agacaaaaca cggcagagca atttgttttt gccctatccg
     3601 atcgtggaaa aatacagcta tacaattaca cttccgttag attacacatt aataaacaaa
     3661 cgctttaacg aacgtctgaa aaataatgta ggaaccgttt ctgttgaaat aaaacctaaa
     3721 ggaaatcaga tagaaatcaa cagagagctt aagctaaaca cgcaaatgat ttatcagatt
     3781 gattataatg aatttagaga tatgatcaat ttctggcttg ataagaatct gagtacggtt
     3841 gttttcaaga aaaaagattg aaaatggctt gttgaataca atactaagat ttacatagat
     3901 aaaccgaacc tttatggtat caccttttac ttaacttgag ggaaaagcta aacttgttga
     3961 atagcagtgc tttattaaat ttatttttgg atttgaggta tcgcacattt tcattatnnn
     4021 nnnnnnngag ctatttagtt attgctgaga atcaaaacgt tacaaataaa tgtttttttt
     4081 aggttgttcg tgatagttaa gaaagtttaa caaaaaattt ttttaaggga aacttgcaca
     4141 ttttcaatta attaattcag gtttcaaata ctttaataac gattttaaga attaattagt
     4201 cttaatttaa attgcgcatt gaatgattac ttatttttgt cgcgaaattc tattttatta
     4261 atcattagga tttaaaatta gaaattttca ttattaacat ttaaaattga ttgttatgag
     4321 tacaatggaa tttaatagcc aacttgcaag tttacaagat aatcttacct attttgcaca
     4381 atcgctcact caaaatgctg atgacgcaag ggatctcgtt caggagacat accttaaagc
     4441 gcttacctat cgggataaat ttgaagctaa tactaacctt aaagcgtgga catacaccat
     4501 aatgaagaat accttcatta ataactatag gcgtaacgtt aagatgagta caaagtttga
     4561 taatactaac gaccttatct atttaaattc cgggagtgca tcggaggagg caacacctga
     4621 ttcgatttta tcccacaaag agatatcaga agtcgttagt aaattagaag acgagcatcg
     4681 gattccattt cagatgcatc atcaaggata taaatacaaa gagattgctg aacatcttga
     4741 cctctcgatt ggcacagtga agagtagaat tttctttagt cgaaaaaagc tgatgaaggc
     4801 tttaaaggaa aattaatgaa taggggcaaa aaggggatta aaagtcccct tttttgttag
     4861 acaaatacag gtttaaataa atcgtaataa gacggaattc atatttttgc aaagttatcg
     4921 ggtttatcgt ttaccctgtc aatggagaga tttgatagat tgcaaccata aaaaatgttg
     4981 aaacaatatg ttttaaaggc tcgccgatca atatttcccc ttgttttatt aattaaaatt
     5041 attaagagtc atgagttatt tatttacttc agagtcagta tcggaaggac atcctgataa
     5101 agttgccgac cagatttcgg atgctctgct cgatgagttt ttaaaacttg atccagaatc
     5161 gaaggttgct tgcgaaactt tggttactac agggttagta gtttgtagtg gcgaggttaa
     5221 aaccagcgga tgggttgatc ctcaagaggt tgttcgtagg gtagttaatc agattggtta
     5281 taacaaggct tcatatcgtt tcgatggtaa tacatgtgga atcatttcaa cgatacacga
     5341 gcaatcggat aatataaata aaggtgttgt aaagcaggtt gcagaagaac aaggtgccgg
     5401 cgaccagggt atgatgtttg gatatgctgt gcgcgaaatg gataatctga tgccaatctc
     5461 tattgagctg gctcatataa tacttcagca attagcggca atccggcacg aaggtaaagt
     5521 gatgacatat ctcggccctg attcaaaatc gcaggtaact attgaatacg atgatgacaa
     5581 tcatcctatc aggattgata ctatagttgt ttcgacgctt cacgatgaat ttgacacaga
     5641 tgaagctatg ctcaggcaaa ttaccgatga tgtaaagaaa tatttgctgc caagagttat
     5701 cagtgaaact cccgagaaat accgccatct tttcaaagat gattataaac tttttgttaa
     5761 tccttcaggg aaatttgtta ttggaggtcc aaatggtgat acaggcttga caggcagaaa
     5821 aattattgtg gacacttatg gcggcaaagt tgcccatggc ggcggcgctt tttcaggcaa
     5881 agatccttca aaggttgatc gttccggagc ctatgctgct cgtcatctgg caaaaaacct
     5941 ggttgctgcg ggagttgctg acgaagcgct tatccaggta gcttatgcta taggtatagc
     6001 agaaccggta ggcctttttg taaatacttt cggcacggca aaagtaaaag ataaaaatgg
     6061 taataagttg agtgatagta agattgctaa aatcattaaa gccaattata aattaactcc
     6121 ttttgaaatt ataaagcgtt ttgagttaaa aagcccaatt tacctcaaaa cagccactta
     6181 tggtcatttt ggaagggatt gttatcataa ggaagtagaa gttttttatg aagatgacag
     6241 cacctttgta aaggtgataa acaataaaaa aaagattttt aagaaggtta agtattttgg
     6301 ctgggaatgt cttgattatg tggatgtttt taaaaaggcc ttcaatatat agcctcaatt
     6361 ctgttgtcct ttccctattg gatatggatg tcttttaatt agctgaaatt acttttcgga
     6421 gtggtttcaa tgattttgat tacaaacttt ttttaaaaac tctataacta gtggctgccc
     6481 acgcgattaa ttcttcaaag ctttgaaaat ctatttgatg tgctaaccat tcgcaaacga
     6541 tgatccgctg gtaacaattg tcaatattta attggcgaaa aaagtttaag ttttcgtttg
     6601 ggtcaactac ttcgtcgcga ttgccgatca caataaaacg tgccgggcat tttccaagag
     6661 ctatttcggg gatatatgca tcaagttgat tgctgtatgt catcgcagga ttgtacaaaa
     6721 ggcaaggcaa tcctaaatcc tcgcctaacc aaaaagagat gtatcccccc agtgaactgc
     6781 cgattaaaaa ctctattttc ctctctataa ttttctgacg gaggttttga taaatatttt
     6841 tttcaatccg ataatcaatt ttaggtgcaa acacttcgaa ccctactttt tccatcattt
     6901 catttttttg agaaaccggc aagctatcta agccatgaag atacagtgtt ttgattttag
     6961 acatgaaatt tatgttttat taataagtaa agtcaataat ataaaagttg taatttgatt
     7021 atttgaacta ttaaaggtat ccgccaagga cagccgtttt tttgtaattt cttttatttc
     7081 cattcagatc gaaagcttca aaaagaataa tatatatgcc gatagcagct ttttgcccat
     7141 gatcagtaat tccattccaa ctaaacgtac cttctgttcc taaaagttca ttttggataa
     7201 gcttccgaat taaccgtcct cgtgtatcga aaatagtgat ggaggcaata aagccgggcg
     7261 attcaaacat ataattgatg ttcaacacat catcttttcc atccatatcc ggagaaaaaa
     7321 tatcgggttg aatagttata ggatcatcaa taaatgttac atcagagtat tgagagtttt
     7381 tatatcctgg tgtggcaaat ccaaaatctt cagcagcaga gtgccagtta gtaatatcat
     7441 ttgatggacg caacgggtta attctttcta atgatactcc cttgtaggat ttaagaagtg
     7501 ggatatgcat attctccgaa tattctaccc gatcaataac ctcatgtcct ttcgttgcaa
     7561 gaatcactat accatctgca ttactgtatt gtggcaaaga ttccatttta gtaaaattat
     7621 ccgggttttc agtataatat tgctgtttta caacatcggg gttggtggta agcgtcatat
     7681 aattttccgg gaaaagtaaa agcccattct tactgatatt tttaggtgtg ctgatttcac
     7741 cggtactttt atcctctgtg gctaaaacca acgtacttag atcaagtatt ttatcggaaa
     7801 tattaaagat ttccacaaaa tcaacacctt tgacaatatc ctctcgagga ttaaacaata
     7861 tttcattgat cacaatgtca ttaggattca caggtctggg gattccgaaa tatgttattt
     7921 ttgatttgtc tattaagttc cctgcgcaat cgaaaaggtc atttaacacc tcaagagcat
     7981 agacaacatc ttctttcaaa ggattttccg gatttagaat gatagctacc cttgaataat
     8041 caggttcttt tactattgct tgtgtcggat atccgatgga gggggagatg tgataactct
     8101 gaggaacatt aagtaatagg ctatccattg gttcggagaa atgtaaaaca atttctgttg
     8161 ttgaaatata ttctactctg gcagtttgag gagctaaaat atctgggttg tttgctttta
     8221 cagagtttgg cgcccccggt gttccgcctg tcgaactgat agaggcagtc caattgttct
     8281 ttccgccgca agggttttcc ggatcaattt gttcgagcga ccagcctccg tcttttttgt
     8341 ttgtatcata ccattcgtcc gaataggtaa cagttgaaat aacctggtct aacatgtttt
     8401 tcaaagtaat cgtctggcca ctatttggta gttgaaatcc tgaaaatcca taaaaaaggg
     8461 catattgtga tagctcttga gctgagtttt cgtgcccgac aagcaaatat tgattaggtt
     8521 gtagctttaa ttgggaaaat actttaatgg tgtttccaat tattaatctc catcccgata
     8581 tattaatttc tttgtcagta gtgtttagga tttcgataaa ttcgacattt ggcaatccca
     8641 cagggggatc ggggtctgcc attatttcat ttatttgtac atcaaatgcc gaagctcgaa
     8701 accacataaa ttctgattcg atagtaggcg cagtatttcc gtttagatca gcaatatttt
     8761 taatcgtgat cgttagcggc atcccgttag ggaaagaacg gtcaaaaagc aacattactt
     8821 tccctggatt ttgggtgtca agctgagcga tcattgggtt ttcaatacta tggcttacaa
     8881 aataattctg tgtgttgata gcttgtgcgg gtaataatgc ctcattaaaa taaatgtcga
     8941 ggttatttgc gcttgttgca tcaatgttaa ccaattgagg tggttcgtta tcaataatat
     9001 gtggccccgc ataaaattgg tcgaaacaaa aatttttgat attgcttact gtatatttac
     9061 aataaacacc cagaaatgag gatgttaata tttgatcatc aaagcctgta gcttcttgtt
     9121 gataaactcc ataccctgta ttatcctttt caattgtcca gttcccataa acatcacgac
     9181 gaattcgaag ccatagttga aaagcgtttg caatcaaacc gtcagttcct ctgcaaatca
     9241 gagttagtgt ttcgccgttt tggcgatata actcaatggc atcattagag aggttttcgc
     9301 caaatcgtaa aaaataacca tttaatggac cattaaggct ttgtttatcg ctggctagat
     9361 aaacacgcgc catattgttg tcagatggag caaaattcat ctttatccaa aattgccact
     9421 cagtttcacc gttcatttca tagtgtgttg ataaatgaga aattcctgct gtggcagcgt
     9481 ttaactgcaa ttgaaaagaa ctgttaacga taaaatcatc aacatcgcca taccacggcg
     9541 ggtcagcagt aaaatcccca tcactaaaat catcatacat ttgagccgat aaaaagagcg
     9601 gtattactaa aaaagttata aaccaaaacc tcatgactat tttgttttaa tgtttgagaa
     9661 aacaaaaact ttttggcatt tttccagata gcgcaaatct acaaaaaaag aaaatctcta
     9721 actaaaaaat cagccctgat aaaattaatt tctgataaac attttcagaa attgttgttt
     9781 attatttacc tactgttgtc attaatttta tttggcgaaa agagtacttt tgccaattgt
     9841 aaatatttag gccccatagt tcaacggata gaatttcaga ttccggttct gacgatgtgg
     9901 gttcgaatcc cgctggggtc actagttata aaaacaatgg ccaaaataaa acggacggta
     9961 ttttattatt tgacatctgt tgttgttgtt gctaattaaa tatgtctttt cggaagttgt
    10021 aattttttgg aatttgatgc ccattttgtt attaacaatt atacgtaaaa caaatagaat
    10081 caaatggata agtttaaaga caaaaattaa ctttcaaatt tattttgctt cttatttcat
    10141 tcttttattt ttttttacat ttgcacaaat tataattatt aaaactatga acattccaga
    10201 caatttaaag tacactaaag agcacgaatg gatacgtatg gatggtgatt ttgccattgt
    10261 tggcattaca gattatgctc agaatgaatt aggcgatatc gtttatgtag aggttgaaac
    10321 tgtaggtgaa acgctggaaa aggaagagac ttatggcact attgaagctg ttaagacagt
    10381 ttctgacctt tttatgcctg taaccggtga gataatagaa tttaatgagg agcttgagga
    10441 caatcctgaa ataataaaca gcgatcctta cggaaaaggg tggataataa aaataaaacc
    10501 tgataatcca tccgatatta tggatttgtt agattctgcc ggatacaaag atttgattta
    10561 aagaaaagct gtaacatcat atagagtaaa ctatacaata tgtacaagca ccgtttcgtt
    10621 tttatttaaa tgcagacaat tgattaaaaa tactacatta tggacgcaaa aaaaactgat
    10681 agagccgacc ttgaaaacaa aaaaaatatt tttttacaga taggtctagt cattgcatta
    10741 ctcttcgtac ttttggcatt caactataag tcttacgaaa gagcaaccgg ctttgatatt
    10801 caagttcaag tggataatgc tcccgaagag attatcccca ttactaaaca agaggcaaaa
    10861 ccacctccac cttcaccccc aaggcaaatt acactaatca atatcgttga agataacgta
    10921 gaggtagagg atgctatcga aattgatgtt gaagctacgc aaatgacaga gattgctgat
    10981 tttatccctc cagtagtaac gaaaggagag gaggtggtag aagagctcga aatatttact
    11041 gtagttgaat caatgcctga atatattgga ggtgatgagg caagaatgag ttttttgcag
    11101 gagaatatta aatatccaca gttggcacgt gagagtggaa ttcaaggtac agtattcgta
    11161 actttcgttg tggaaactaa tggtagcgta actgatgtaa gggttttacg tggtataggc
    11221 ggtggatgtg atgaagaagc cgttagagta gttaagatta tgccaaaatg gaaaccaggt
    11281 atgcagcgca gtaaaccagt gcgcgtacaa tttactatgc ctattagatt cacactacaa
    11341 ggataacact cgaagtagat acccaatagt attaataata actgaataaa aatttcccgg
    11401 atattttaaa tattcgggat ttttttatct ctattttagg gtgtaacaaa acgatattat
    11461 agtaaaagca atgtttggga taggattttg aacttatatt aagcatttga taaacactta
    11521 atcgtggttg gctacataat taatatttag ctcaattggg ctctctattg attaatttcg
    11581 gattgaaaat aattggatga aatcatgtcg tttaatatct gattatgatc aaaggccaat
    11641 tctggcaatc tgtttattgg aaaccaagct gctccacttg catcatcacc tccaatcacc
    11701 gatgaatttt caggtgtagt gaaaccataa aaaacaactg agattgttct gccacgtgga
    11761 tcgcggtgcg gatcgccgta tgatttaaat tggtggaggt tgatattttc aagccctgtc
    11821 tcttctttaa gttcgcgcct gccagtttct aaaagagtct cttccatttc aataaatccg
    11881 cccggcagtg cccatttttc tctaaaaggt tctttccctc gttgaataag gagtacgctc
    11941 atttctttat caaacataca aaacactatg atgtcaactg ttacagaagc acgaggataa
    12001 tcataaacat attgcataaa gtaaaagatt tagtttaagt gattaatgta atgaggtttg
    12061 agttcacttt taaaacaatt ttttcggata ttcgtgtatt tgtggctaaa agtacttttc
    12121 ggagcggatt taagggataa aagtcggaat ttctatttta gtccatcaac aacaatgaca
    12181 aattcacctt tgatggcgct ttgttcaaaa tgaaggatga tctcttgcag ggtgcctctt
    12241 attgtttctt cgtaaatctt agtcaattca cgcgacactg atgcttttct ttctttactg
    12301 aaataaccgg caagttgttc aagtgtcttg agtagccgat gtggtgattc gtaaatgatt
    12361 acagtaaacg gtagttcgct caaaaatttt aatcttgtag tccgtccttt ttttgttggc
    12421 aaaaatcctt caaaaaagaa cttatctgcc ggtaatcccg aatttatcaa tgcaggtaac
    12481 agggctgtag gtccaggtaa ggtttcaaca gggatggcat tttttaaaca ttctctgatc
    12541 agtaggaagc ctggatcgga aatagcaggc ataccagcat cgcttactaa cgcagctttt
    12601 tttccttcag caatttgtga acaaagctta ttcaccaatt gatgttcatt gttttgatga
//
LOCUS       DAOOAI010000068        12366 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_6572_length_12366_cov_5.754366, whole genome shotgun sequence.
ACCESSION   DAOOAI010000068 DAOOAI010000000
VERSION     DAOOAI010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12366)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12366)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12366
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6572_length_12366_cov_5.754366"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(50..1156)
                     /locus_tag="PK345_06055"
     CDS             complement(50..1156)
                     /locus_tag="PK345_06055"
                     /inference="COORDINATES: protein motif:HMM:NF016146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease"
                     /protein_id="HPX34612.1"
                     /translation="MFFIASSAIAQPINYYDAAQGKTGEALKTALHNIIKGHTVVSYD
                     SIWTAFQTTDKRADGKVWDIYSNCNFDFGINQCGNYTDECDCYNREHSVPGSWYSDGA
                     PMNSDIFHLIPTDGKVNNIRSNYPYGKVDNPSTTTLNGSKLGSCSWPGYSGTVFEPVD
                     EYKGDIARIYFYMATRYEDVISGWYNNSPQADAALQNNSFPVFENWFLEMLGEWHVND
                     PVSQKEIDRNNAVYAIQQNRNPFVDHPEWVYDVWSVGFMLQPEPDQHVTNFSAHTITL
                     QWSDAQGPVLPDGYLIRMSNTGFENIMTPQDGTTITDDFWNKNVSYGAQKATFGELTP
                     GTLYYFKIFSYSGNGNDIDYKTDGEIQQVSIKAR"
     gene            complement(1412..3403)
                     /locus_tag="PK345_06060"
     CDS             complement(1412..3403)
                     /locus_tag="PK345_06060"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HPX34613.1"
                     /translation="MNDFQKAILQGLPDTLPAPRQYDTTVPHAPVRKDILTADEKKLA
                     LRNALRYFDKKHHAALAPEFLNELKTYGRIYMYRFRPTYKMYARPLNEYPYQSKQAAT
                     IMLMIQNNLNPDVAQHPHELITYGGNGAVFQNWAQYLLTMQYLATMTDDQTLAIYSGH
                     PLGLFPSHPDAPRVVVTNGMVIPNYSKADDYERFNALGVSQYGQMTAGSYMYIGPQGI
                     VHGTTITVMNAGRKTSSGNEDLGGRLFVSSGLGGMSGAQAKAAVIAGAIGVIAEINEK
                     AAKKRHNQGWVDELHYDMDKLMKRIKEAQQKKEAISLAFVGNIVDLWEKFADEQIKVD
                     LGSDQTSLHNPWAGGYYPAGLTFEESNKMMVADPKAFKEHVQASLRRQVAAINRLTEN
                     GMYFFDYGNAFLLEASRAGADILNDKGGFRYQSYVQDIMGPMCFDYGFGPFRWVCASG
                     NPDDLDKSDQIAIEVLEKIAKNAPEEIALQLKDNIRWIKEAKKNKLVVGSQARILYAD
                     AEGRIKIAEAFNNAVRQGILTAPVVLGRDHHDVSGTDSPYRETSNIYDGSQFTADMAI
                     QNVIGDSFRGATWVSIHNGGGVGWGEVINGGFGMTLDGTKEADRRLKMMLHWDVNNGI
                     ARRSWARNKGAIFAIKRAMKQNPKLKITMPNMVDDKLLE"
     gene            3691..3861
                     /locus_tag="PK345_06065"
     CDS             3691..3861
                     /locus_tag="PK345_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005810660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HPX34614.1"
                     /translation="MAYVINDDCIACGTCIDECPVEAISEGDIYKIDPEICTDCGSCA
                     EVCPVEAIHPEE"
     gene            complement(4033..5574)
                     /gene="rny"
                     /locus_tag="PK345_06070"
     CDS             complement(4033..5574)
                     /gene="rny"
                     /locus_tag="PK345_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HPX34615.1"
                     /translation="MQILICSLIGVAGLALGVILSTTILRKKLEEKGRRTLEEAQEKS
                     EILKKEKLLQAKEHFLKLKTEHDQVVNEKNANILRNENRLKQKETSLNQKLEENNRKE
                     KELVLIRKNLTTQLEILEKKQQEVDKSLQRQREQLENISGLSTEEAKAQLVESLKDDA
                     RAEAMTHIQEIMDEAKLTANQEAKKIIVETIQRTAAENTIENTVSVFNIDNDEIKGRI
                     IGREGRNIRTLEALTGIEIIIDDSPDAIILSGFDPVRREIARLSLHKLVTDGRIHPAR
                     IEEVVSKTKTQIEQEIVELGKRTCIDLGIHGMHPELVRLIGKMKYRSSYGQNLLQHSR
                     EVANLCATMAAELGLNPKKAKRAGLLHDIGKVPDNESELPHALLGMKLAEKYKENPEI
                     CNAIGSHHNEVEMETLLAPIVQVCDAISGARPGARREVIEAYIQRLKDLENIAMSYPG
                     VLKTYAIQAGRELRVIVSSENITDDQANVLAHELSKKIQSELTYPGQIKITVIRETRA
                     ISFAK"
     gene            complement(5862..6155)
                     /locus_tag="PK345_06075"
     CDS             complement(5862..6155)
                     /locus_tag="PK345_06075"
                     /inference="COORDINATES: protein motif:HMM:NF017016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HPX34616.1"
                     /translation="MNNILITVRVADREYRLNIEREKEELVREAVKRINETIKRYAEN
                     YDFKDKQDLLAMVVLQNTIKAMELDLQVSFQQNQLASKLIEIDRLLTESLSGK"
     gene            complement(6176..6463)
                     /locus_tag="PK345_06080"
     CDS             complement(6176..6463)
                     /locus_tag="PK345_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34617.1"
                     /translation="MVDLITLVSGIEFRIRELISRYDAIQNENEQLKSNNEQLLRELD
                     ELKHSIKQLQYKNQIIKIAKAIEKEKGSTNAKYLINELLREVDRCIGLLND"
     gene            6744..8024
                     /locus_tag="PK345_06085"
     CDS             6744..8024
                     /locus_tag="PK345_06085"
                     /inference="COORDINATES: protein motif:HMM:NF022466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2851 family protein"
                     /protein_id="HPX34618.1"
                     /translation="MFEAFLHFIWKYQLYSTSLFTEAGEPISVISPGIHNRDSGPDFT
                     DARILIGNTLWAGNVEIHVKASDWNLHGHSTDKAYSNVILHVVYEDNIPIYDSNGRQL
                     DSLVMKGKFSEKIYHKYYYFLNNSNWIPCEKDIHYVPPEKIRLWLESLLISRMERKAN
                     ELKALLEPNLNDFEETFYQALAANFGFKVNQQPFAMLARMLPLQILEKHVGSQFQIEA
                     MLFGVSGLLSNNFNDNYPNRLKKEFEFLQKKYGLVSMQAHLWKFMRLRPPNFPTIRIS
                     QFAALINKSSRLFSAIIETDDPKSLISLFDVSAANYWNDHFTFDKPSPGQKKNLGTQA
                     IDNILINTVTQMLFLYSISTGAEQYRDKALALLSAIGPESNSIIKKWNILGIESTNAF
                     DSQALIELKNRYCKLKRCLDCSIGLDLLKMNSKG"
     gene            complement(8081..12310)
                     /gene="dnaE"
                     /locus_tag="PK345_06090"
     CDS             complement(8081..12310)
                     /gene="dnaE"
                     /locus_tag="PK345_06090"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HPX34619.1"
                     /translation="MYLIFDTETTGLPRDHSAPLTEFSNWPRMVQIAWQLHDNQGDLI
                     EAESYIIQPDGFTIPYNAEKIHKISTQIAQEHGVPLAEVLHKFREVVKQSTHLVGHNI
                     EFDLKVVGSEFLRAGQDNPLDTNKMLDTMIVSVGHCALPGGRGGGYKYPTLEELYEKL
                     FGSAFMTAHNAIADVAATARCLFELIRINVIDHKVLKIDEEIVTTFRNNHPNVIPPYE
                     TAESDRIKSYYGIISSKDDTLKESNDDVLIEEFTHLHVHSHFSILEATATPADLLDKA
                     AEFEMEALALTDHGSLFGAFEFHSEARQRNIKPIIGCEFYLVQDHTKLKFTKDNPDRR
                     SFQVLLAKNLTGYRNLVKLSSAGWIGGLYDNKPRIDKELILKYKEGLIALTGSIYSDV
                     NDLILNFGETQAEETFIWWKEHFGDDFYVQLNRHGLPEEERTNSVLIHLAKKHNVKLV
                     AANNVYYIEKEDADIHDCLLCIKNGEFQSTPIGWGRGTRFGFPNDEFYFKSKKEMSEL
                     FSDLPEALRNTKEIVDKVEVYDLESKPIMPHFDIPNEFADADDYLRHITYRGAAERYE
                     VITDDIRQRIDYELETIKKMGYPGYFLIIEDFLRKARNMGIWVGPGRGSAAGSVVAYC
                     LKITNIDPLKYGLMFERFLNHDRISLPDIDTDFDEDGRELILKWVADKYGHNRVAHII
                     VFGKMAPKMAIRDVARVKQLPLSEANRLAKLIPAKPGITFPKAYQQVPALNKEKNSTN
                     TLIAQTLAVAEKLEGTIRNTGTHACGIIISKDDLIDHIPVSIAKDTELLVTQFDGVNI
                     EKSGMLKMDFLGLKSLSIVKDTIELVKKYHGVDIDIYSIPHDDEKTYELYSRGDTTAI
                     FQFESEGMKRSLRKLKPSRFEDLIAMNGLFRPGPMDYIPNFISRKHGMEKIEYDIPEM
                     EEVLKETYGITVFQEQVMQLSQRLAGFTGTQADTLRKAMGKKISDLTAELKPLFFEGG
                     IKNGYDEQALQKIWDDWSKFTAYAFNKAHSTCYAYLSYRMAYLKAHYPAEFMAAVLSR
                     NLNDITKITLLIDEVKQMGINVLKPDVNESDLTFVVNKNKDIRFGLGAIKGMGTAVAE
                     AIIQERNDNGPYKSIFDFARRVNLKMVNKRGFEALAMAGAFDSFQDMHRAQLFFKEEA
                     ENQLFLEKIMRYAQKFQEQQNSSQISLFGDITETTIEDPKLPQCKPWSKFEQLQYEKA
                     VTGFYITGHPLDEYKLEITHFSNCNISDFKDNMPDFSGRTLKIGGMVTEASHLTSKDN
                     KPFGGFIIEDFSSSLEIKLFREDYLKFKHFLTEGIFLHVTGQIQKRYSNDAFYDFKVI
                     NMQLLSEIIEKQTKNIGLIIPIETLTAELINKIFSNATAMRGKCKIYFQIVDGMERLK
                     LNLSNANVSVNPLPFLKFVSKLKEIDYVINK"
BASE COUNT         3181 a         2631 c         2489 g         4065 t
ORIGIN      
        1 tttcataatt ttttaaaatt taagtttatt aattatttct tttttttctt tatcttgctt
       61 taatacttac ctgttgtatc tctccatctg ttttgtagtc aatgtcgttt ccgtttccgc
      121 tgtagctaaa tattttaaaa taatagaggg tgccgggtgt cagctctcca aaggttgctt
      181 tttgcgcgcc ataactaaca tttttattcc agaagtcgtc ggttattgtt gttccgtctt
      241 gcggcgtcat aatattttcg aagcctgtgt tgctcattcg tatcaggtat ccgtcgggca
      301 aaacggggcc ttgggcatcg ctccattgca gtgtaatggt atgtgccgag aagttggtaa
      361 cgtgttggtc gggttcgggc tgaagcataa agcctacact ccatacatca tatacccact
      421 caggatggtc aacaaaaggg ttgcggtttt gctgaatggc gtaaacggcg ttgttgcggt
      481 caatctcttt ttgcgaaacg gggtcgttta catgccactc tccaagcatt tccaaaaacc
      541 agttttcaaa caccggaaag ctgttgtttt gtagcgcagc atcggcttgc ggcgaattgt
      601 tataccatcc cgaaatcaca tcctcgtatc gtgtagccat ataaaaatag atgcgtgcaa
      661 tatcgccttt gtattcatcc accggctcga acaccgttcc gctataaccc ggccaactgc
      721 atgagccaag cttactgccg tttagggtgg tggtggaagg gttgtcaacc ttaccataag
      781 ggtaattgct tcggatattg tttaccttgc catcggtagg gatgaggtga aatatatcgc
      841 tgttcattgg cgcgccgtca gaataccagc ttccgggaac agaatgctca cggttgtaac
      901 agtcgcactc gtcggtgtaa ttcccgcatt ggttaatgcc gaagtcgaag ttgcaattgg
      961 agtagatatc ccatactttg ccgtctgctc ttttatcggt ggtttgaaat gcagtccaaa
     1021 tgctgtcgta gcttactact gtatgccctt ttattatatt gtgtaatgct gtttttagcg
     1081 cctcgccggt ctttccctgc gcagcatcgt aatagttaat aggctgagca atagctgatg
     1141 atgctataaa aaacatagca acaaaaagtt taatacggca tttgtagatt ttcatcaaat
     1201 tgatgacgga aatgatttgg cgcaaaaata agaaatttcc ctatgcgaaa atgacaattt
     1261 ttaaactttt taacaaaaat taatcaacag aaaattctta aatgttgagc tattgattag
     1321 agcttgtatt tcatggcgcg ccattttgcc atgtcattct ttttagtctt aaaagttgac
     1381 ttcaagtatt atcagatgtc tttatccaaa tctattccaa caacttgtca tccaccatat
     1441 ttggcatcgt aattttcagt tttggatttt gtttcatagc tcgttttatg gcaaatattg
     1501 cgcctttgtt tcttgcccag ctacgtcttg ctattccatt gtttacatcc cagtgcagca
     1561 tcattttcag tcttctgtcg gcttcctttg tgccgtcgag tgtcattccg aagccgccgt
     1621 ttatcacctc tccccatcct actccgcctc cgttatgaat tgatacccaa gtggcgccac
     1681 ggaatgagtc gcctattaca ttttgtatag ccatatcagc agtaaactgc gatccgtcgt
     1741 agatgtttga agtttcgcgg tagggcgagt cggttcctga gacatcgtga tgatcgcggc
     1801 ccagcacgac cggcgctgtg aggattcctt gtctgacggc attgttgaaa gcttccgcta
     1861 tctttattct cccttcggca tcagcataaa ggatacgagc ttgcgagccg acgaccaatt
     1921 tgttcttttt agcctccttt atccatctga tgttgtcttt caattgcaaa gcgatctctt
     1981 ccggagcatt tttggcaatc ttctctaaaa cttcaattgc gatctggtcg cttttatcca
     2041 aatcgtccgg gttacctgag gcgcacaccc atctgaatgg cccgaatccg tagtcgaaac
     2101 acattggtcc cattatatcc tgcacgtaag attgatatct gaacccgcct ttgtcgttca
     2161 gtatatcggc tccggcgcgg cttgcctcca acaggaaagc gttgccatag tcgaagaaat
     2221 acatgccatt ttcagttagc cggttaatag ctgctacttg ccgccgtagc gatgcttgta
     2281 catgctcttt gaatgccttt gggtcggcta ccatcatttt atttgactct tcaaaagtaa
     2341 ggcctgcagg gtagtagccg cctgcccatg gattgtgaag cgaggtttga tcggagccga
     2401 ggtcaacttt tatttgctca tcagcaaatt tttcccacag gtcaacgata ttgccgacaa
     2461 aagctaacga tatggcctct ttcttttgtt gcgcctcttt tatcctcttc atcagtttgt
     2521 ccatatcata gtgaagctcg tcaacccatc cttggttatg tcgttttttt gccgcctttt
     2581 cgtttatttc ggctattact cctatagctc ctgctattac ggcggccttt gcctgagcgc
     2641 ctgacatgcc tcccagaccg gatgatacga agagcctgcc acctaagtct tcatttccgg
     2701 aacttgtttt cctgccggca ttcatcaccg tgatagtggt gccatgtaca attccttgcg
     2761 ggccaatata catgtacgat ccggcggtca tctgcccata ttgcgatacg cctaaggcat
     2821 tgaaacgctc ataatcgtcg gcttttgagt agttggggat caccattccg ttagtaacaa
     2881 ccacacgcgg agcatcgggg tgcgagggga aaagccctaa cggatgaccg gaatagatgg
     2941 caagggtctg atcatcggtc atggttgcca aatattgcat agtaagcagg tattgagccc
     3001 agttttgaaa tacggcgcca tttccgccgt aagttatcag ctcatgcgga tgctgtgcta
     3061 catccggatt gaggttgttc tggatcataa gcataatggt cgctgcctgc ttcgactggt
     3121 atggatattc attcaaaggc cgtgcataca ttttgtatgt gggtctgaaa cgatacatat
     3181 aaatcctgcc gtaggtctta agctcgttaa gaaattcagg ggcaagggcg gcatgatgct
     3241 tcttgtcgaa ataacgaaga gcattgcgca gagccagttt tttttcatcc gcagtgagaa
     3301 tgtccttacg caccggagca tgtggaaccg ttgtatcata ttgccgtggc gctgggaggg
     3361 tgtcgggtaa cccttgcaaa atggcttttt gaaaatcgtt catcgtttaa atatttagta
     3421 agatgtttaa ctttttgcaa aattaacccg aattaatcaa tagatgacct tttttatgct
     3481 gaactattga gtttaagtat tataaaatgg ttggtatgtt tttttcttta ttttcgggta
     3541 tttgtgtctg aaattattca tcggagccgg catgataaat acaatttaga ctggctataa
     3601 atttttgaaa aaaaggttta aatcagattt tataatagaa aatatgttga cctttgcaat
     3661 cacattgttt aactcattaa aaataaaaac atggcttacg taataaatga cgattgtatt
     3721 gcttgcggta catgtattga tgagtgccct gttgaagcaa tctctgaggg cgatatctac
     3781 aaaattgatc cggaaatttg caccgattgt ggctcttgcg ccgaagtgtg ccccgttgaa
     3841 gcaatacacc ctgaagaata agtaaggttt agatattttc cggaaaatag tttgcctcag
     3901 aggtctttaa tcgttttgaa gaaagagctc tgaggcattt ttatttgtag gcagcgccgg
     3961 aaacaaaggt tagaatttac cttttgtgct gttttcctca gtcaatgatt tcacttttat
     4021 atcttttcag tgctattttg cgaagctgat tgctcttgtt tcccttatca ccgttatctt
     4081 tatctgtccg gggtaggtta gttcgctttg tatctttttc gacagttcgt gtgccagcac
     4141 atttgcttga tcgtcggtga tattttccga agatactatt actctaagct ccctgccggc
     4201 ctgtattgcg tatgttttca atactcccgg atacgacatt gcaatgtttt ctaagtcttt
     4261 aagtcgttgg atataggctt caattacttc gcgtcttgcg ccgggccttg ctcctgaaat
     4321 agcatcgcaa acctgtacta ttggcgcaag cagggtctcc atctccactt cgttgtgatg
     4381 cgagccaatg gcattacata tctcaggatt ctctttatat ttttcggcca acttcatacc
     4441 taacagcgca tgtggcagct ccgattcatt atccggcact ttgccgatat cgtgtaacaa
     4501 acctgcccgt ttggccttct ttgggttaag accaagctcg gcagccatag ttgcgcatag
     4561 gttggctacc tcgcgcgaat gttgtaacaa attttgaccg taagaggagc ggtatttcat
     4621 cttcccaata agcctgacta attcgggatg cataccatgt attcctaagt caatacatgt
     4681 acgttttcct aattccacta tctcttgttc tatctgtgtc ttggttttgc tcactacctc
     4741 ttcgatacgt gcggggtgga tacgtccatc ggtaacgagc ttgtgcaacg acaagcgggc
     4801 tatctcccgt cgtaccggat cgaagcctga aaggataatg gcgtccggcg aatcgtcaat
     4861 gattatctcg atgcctgtca aagcctccaa tgttcggatg tttcgacctt cgcgtccgat
     4921 tattcgtcct ttgatttcgt cattatcaat attaaagacg gaaacggtat tttcgatagt
     4981 gttttcggca gccgttcgtt ggatggtctc tacgataatc tttttagcct cctggtttgc
     5041 ggtaagcttg gcttcatcca ttatttcctg aatatgtgtc atcgcctccg cacgagcatc
     5101 atctttcaag ctctcaacaa gctgagcctt ggcttcttct gttgaaaggc cagatatgtt
     5161 ttcgagctgt tcgcgttgtc tttgcaacga tttgtcaacc tcttgctgtt tcttctcaag
     5221 gatttcgagt tgagtagtta ggttttttct gataagaact aattctttct cttttctgtt
     5281 gttttcttca agtttttggt ttagcgatgt ctctttttgt ttaagccggt tttcatttct
     5341 caggatgttg gcattttttt cgtttaccac ctgatcatgt tcggttttta gttttaagaa
     5401 gtgctctttg gcctgtaaca gtttctcttt tttcaggatt tcactctttt cttgagcctc
     5461 ttccagcgtc cttcttcctt tttcttccag cttttttcta agtatggttg ttgataggat
     5521 aactcctaaa gcaagccccg ctaccccgat aagtgaacaa attaatattt gcatcataaa
     5581 aataaagttt aaggttagcg gcaattggaa ataaaaaaac ccgcattaat tacaatcgga
     5641 atttagtaaa ccccagtctg aatgattagg cttaccgcag gctttgaact tccactgtcc
     5701 gccgtagcga atttcccgaa ggaaatgagg cccgtcctac acctgctgag aattccctaa
     5761 agtgataaat gttgagttta caaatattaa aagaattaat gcgggagtat ctttcagtat
     5821 caatacaatt gatgcttatt atcaaagaac gttgtggcga actatttgcc tgacaaactt
     5881 tcggtaagga gtctatctat ctcgattaac ttgcttgcta actgattctg ctgaaagctg
     5941 acctgaaggt ctaactccat ggctttgatt gtgttctgca aaacaaccat agccaataag
     6001 tcctgcttat ctttgaaatc atagttttca gcatatcttt ttatggtttc gttaattctt
     6061 tttactgctt ctcttacaag ctcctccttt tccctttcta tattaagacg atattctctg
     6121 tcagctaccc tgactgtaat caaaatgtta ttcatcagga ttatttgaaa atatttcaat
     6181 catttaaaag accgatacat ctgtcaactt cacgcaaaag ttcatttatt agatacttcg
     6241 catttgttga tcctttttct ttctcaattg cctttgcaat ttttattatc tggtttttat
     6301 attgtaattg tttaatacta tgttttagtt cgtccaactc tcgtagcaac tgttcattat
     6361 tacttttgag ttgttcgttt tcgttttgaa tagcatcgta acgggaaatc aattctctta
     6421 ttctgaactc aataccactt actaaagtaa taagatcaac cattcaacaa cttccgtttc
     6481 ggtgtttttc ttagacctga caaagttata caaaaatttg atcttgaaat aaaaattgcc
     6541 agaaatattt tactgatcac taataaatca cacgaattgt taaacgaaga atgaatagat
     6601 aatgtgttta atttcagcaa gataatctgt caaaaagttc atccgtagat taaaaatcat
     6661 cattattaaa acatgtaaat aaaaaaaggt tgattgatcg ccttgagata ttttagtagc
     6721 tttgttaaaa aaaatacctg cagatgtttg aagccttttt gcattttatc tggaaatatc
     6781 aactctactc cacatcgctt tttaccgaag cgggcgagcc gatttctgtt atttcgccag
     6841 gcattcataa ccgtgattca ggccctgatt ttactgatgc aagaatactg ataggcaata
     6901 ccttgtgggc tgggaatgtt gagatacatg ttaaagcatc cgattggaac ctccatggac
     6961 attccaccga taaagcatac agcaacgtga ttttacatgt ggtgtacgaa gataatatcc
     7021 caatttatga ttccaacgga cgccaattgg actccttggt tatgaaagga aagttcagtg
     7081 agaaaatcta ccacaagtat tattactttc tcaacaattc gaactggata ccgtgcgaaa
     7141 aggatattca ttatgtgccg cctgagaaga tcagactatg gttggagagc ctcctaatat
     7201 cgaggatgga gcgtaaggct aatgagttaa aagcgctgct cgaacccaat ttaaacgatt
     7261 ttgaagagac tttttatcag gcgttggcgg ccaattttgg atttaaagtg aatcagcagc
     7321 cttttgctat gttagccaga atgctcccat tgcaaattct cgaaaaacat gttggcagcc
     7381 aattccagat tgaggcgatg ctatttggcg tgtcagggct gctttcgaat aactttaacg
     7441 ataattaccc gaacaggctg aaaaaagaat ttgaattcct gcaaaagaag tacggccttg
     7501 tttccatgca ggcgcatttg tggaaattta tgaggcttcg gcctcccaac ttcccaacta
     7561 tcaggatctc tcagtttgcc gccctgatca acaagtcttc acggttgttt tctgcaataa
     7621 ttgaaactga tgatccgaaa agccttatct ccctttttga tgttagtgca gcaaactatt
     7681 ggaacgatca ttttactttc gataaaccct cgccaggcca aaagaaaaac cttggcacac
     7741 aagctattga taatatttta ataaacacag taacccaaat gctttttctt tatagcatat
     7801 ctaccggcgc agaacaatat cgcgataaag ccttggctct tctgtcggca atagggccgg
     7861 aatcgaactc tattattaaa aaatggaaca ttttagggat tgaatctact aacgcctttg
     7921 attcacaggc gcttattgaa ttgaaaaatc gttattgtaa attaaaaaga tgccttgatt
     7981 gcagtattgg ccttgatttg ctgaaaatga actccaaagg atgatattgg gttttattat
     8041 ttatatgtga gaagcagatt gaaggattat cacctaaaat ctatttattt atcacataat
     8101 caatctcttt tagctttgat acaaatttca gaaacggcaa gggattcacg cttacgttag
     8161 cattgctcag atttagcttc aacctctcca ttccatctac aatctgaaag tatatcttgc
     8221 atttgccacg catagcggta gcgttgctga atattttgtt tataagctct gctgtaagtg
     8281 tttcgatggg tatgataagc ccgatgtttt ttgtctgttt ttcgatgatc tcagataaaa
     8341 gctgcatgtt gatcactttg aaatcgtaaa aagcatcgtt actatatctt ttttgtatct
     8401 gaccggtaac gtgtaagaat ataccctcgg taaggaaatg tttgaatttt aagtaatctt
     8461 ctctaaacaa ctttatttct aaactgctgc taaagtcttc gatgataaac cccccaaacg
     8521 gtttgttgtc cttcgacgtt aggtgtgaag cttccgttac catgcctcct attttgagcg
     8581 ttctgccgct aaaatcgggc atgttatctt taaaatcgga gatattgcaa ttggagaaat
     8641 gagttatttc taatttgtac tcatctaatg gatgtcctgt aatataaaag ccggtaacag
     8701 ccttttcgta ttgtagttgt tcgaattttg accatggttt acattggggc agtttcgggt
     8761 cttcgatggt tgtttcagta atatcgccaa atagcgatat ctgagaggag ttttgttgct
     8821 cttgaaattt ttgtgcgtag cgcattattt tttctagaaa taattgattc tcggcctctt
     8881 ctttaaaaaa tagctgagcg cgatgcatat cctgaaatga gtcaaaagct cctgccatgg
     8941 ctaaggcttc aaatccgcgc ttgttcacca ttttaaggtt tactcggcga gcaaaatcaa
     9001 atatagattt atatggtcca ttatcgttac gctcctgaat tattgcttcg gcaacggctg
     9061 ttcccatacc tttaattgcg ccaagcccga aacgaatgtc tttgttcttg ttaaccacaa
     9121 aagtaagatc tgactcattc acatccggct tcaacacgtt gatacccatc tgttttactt
     9181 catcaataag aagggtaatc tttgttatgt cgtttaaatt gcggcttaat actgctgcca
     9241 taaactcggc cggataatgt gctttaaggt aagccatccg gtaggatagg taggcataac
     9301 aggttgaatg ggctttattg aaggcgtatg cagtaaattt cgaccagtcg tcccaaattt
     9361 tctgtaatgc ttgttcatca tatccgtttt ttatgccgcc ttcaaaaaac agaggtttaa
     9421 gctctgcggt caggtctgat atcttttttc ccattgcctt acgcagtgtg tctgcttgtg
     9481 tgccggtgaa tcctgccagg cgttgtgata gctgcatcac ctgttcctgg aaaacggtta
     9541 tgccgtatgt ctctttcagc acctcttcca tttccggtat gtcatattct atcttctcca
     9601 taccatgctt tcgggaaata aaattgggga tatagtccat tgggccggga cgaaatagcc
     9661 cattcatggc tattaggtct tcaaaccggc tcggttttag cttacgcagg ctccttttca
     9721 tcccttccga ctcgaactgg aatatggcgg ttgtgtcgcc acggctgtat aattcgtatg
     9781 ttttctcgtc atcatgaggg atcgagtaaa tatcaatatc tacgccatga tattttttaa
     9841 ccagctcaat tgtatcttta actatagaga gggattttaa gccgaggaag tccattttta
     9901 acattcccga tttttcaata tttacaccgt cgaactgggt aaccaatagt tccgtatctt
     9961 ttgcaatact cacagggata tgatcaatca ggtcatcttt acttattatt attccgcatg
    10021 catgagtgcc ggtatttcta atagtgcctt ccagcttttc ggcaacggcc agggtttgag
    10081 cgatcagagt attggtggaa tttttctctt tatttaacgc gggcacctgt tgatatgcct
    10141 tcggaaatgt tattcccggc ttggcaggaa taagctttgc aagccgattg gcctctgaaa
    10201 ggggtaattg cttcacacgt gccacatccc gaatagccat ttttggggcc atttttccaa
    10261 aaacgataat gtgagccacg cgattatgtc catacttatc ggccacccat tttaaaatta
    10321 gctcgcggcc atcttcatcg aaatcggtgt caatatcggg taaagagata cggtcatggt
    10381 tcaaaaaccg ctcaaacatc aacccatatt tcagtggatc aatatttgtg attttcaaac
    10441 aataagccac taccgatccg gcggccgatc cacgtccggg ccctacccaa atacccatgt
    10501 tgcgagcctt tcttaaaaag tcttcaataa tcagaaaata accgggatat cccatctttt
    10561 ttattgtctc caactcgtaa tcaatccttt gccttatatc gtctgtaatc acttcataac
    10621 gttcagccgc tccgcgatat gtaatatgcc gcaagtaatc atctgcatcg gcaaactcat
    10681 tagggatatc gaaatgaggc ataatgggtt tgctctccaa gtcgtaaact tcaactttat
    10741 ctactatctc ctttgtgttg cgtaaggctt caggaaggtc ggagaatagc tccgacatct
    10801 ctttcttgct tttaaaatag aattcgtcat tagggaatcc aaaccgtgtc cctcttcccc
    10861 atcctattgg cgtgctttga aactcgccat ttttgatgca tagtaggcag tcgtgaatgt
    10921 ctgcatcctc tttctcgatg taatagacat tgttggcggc aacgagtttt acattatgtt
    10981 tttttgccaa atggatcagg acactgtttg ttcgctcctc ttccggcagg ccatggcggt
    11041 tgagctgaac gtaaaaatcg tcgccaaaat gttctttcca ccaaataaag gtctcttcag
    11101 cttgtgtttc tccaaagtta aggatcagat cgttaacatc ggaataaatg ctacccgtaa
    11161 gcgctatcaa gccctctttg tattttaata tcaactcttt gtcaatcctt ggcttattat
    11221 cgtacaagcc ccctatccac ccggctgagc taagcttcac aaggtttcgg tagccggtaa
    11281 ggttttttgc aagtaaaact tgaaacgatc ttcggtcggg attatctttg gtaaatttga
    11341 gcttagtatg atcctgtact aaataaaact cacaacctat tataggtttt atattccttt
    11401 gtcttgcctc cgaatgaaat tcaaatgcgc caaacaggct cccatgatca gtgagtgcga
    11461 gcgcctccat ttcaaactca gcagctttgt ctaacagatc ggctggtgtg gcagtagctt
    11521 caagtatcga aaagtgggaa tgtacgtgta aatgcgtgaa ctcttcgatg agtacatcat
    11581 cattgctctc tttaagagta tcatctttag aactaattat gccgtaatag gattttattc
    11641 tgtccgattc tgctgtttcg taaggtggaa taacgtttgg atgattattt ctgaatgtag
    11701 ttacgatctc ttcatctatc tttaatactt tgtgatcaat tacatttatt ctgatcagtt
    11761 cgaacaggca acgtgcagta gcagcaacat cggctatagc attgtgagcc gtcatgaagg
    11821 cgctgccaaa tagtttttca tataattctt ctaatgtagg gtatttgtat ccaccgcctc
    11881 tgccccctgg cagtgcgcaa tggccaaccg acacaatcat agtatccagc atcttattgg
    11941 tgtctaaagg gttatcctga ccagcgcgca aaaattcaga gccaacaact tttaagtcaa
    12001 attcaatatt atgaccaaca agatgagtag attgttttac aacctctcgg aatttatgga
    12061 gaacctctgc aagtggaacg ccgtgctcct gagctatttg tgtagatatc ttatgaattt
    12121 tttccgcatt gtacggtatt gtaaatccat cgggttgtat aatgtagctt tccgcctcaa
    12181 taagatcgcc ctgattatca tgtaactgcc aggcaatctg taccatcctt ggccagttgc
    12241 taaattcagt taatggagcg ctatgatcgc ggggaaggcc agtagtttcg gtgtcgaata
    12301 tcaaatacat agctaaaaat cggatttgga tgcagataaa attattatga caaaattagc
    12361 taaaac
//
LOCUS       DAOOAI010000069        12231 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_6650_length_12231_cov_5.258706, whole genome shotgun sequence.
ACCESSION   DAOOAI010000069 DAOOAI010000000
VERSION     DAOOAI010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 12231)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12231)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12231
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6650_length_12231_cov_5.258706"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2712
                     /locus_tag="PK345_06095"
     CDS             <1..2712
                     /locus_tag="PK345_06095"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34620.1"
                     /translation="LNVKGNWTNSIFPAGFDAGSSRVRFTGPGHQYVTTSETFNILEV
                     ANGAALRVDASTSEVTCNQYDWTSGGIDVIKGTFTALDLAQNGIYGSYYVNPGGVINL
                     EKINGWVDFNGHIYIFGGEFNIYGGTSDSYWPYQANASLTMSGGTLDFKDVGIVVYNS
                     NSFTFTHDITGGTIRTIGGLTVYRSDFTPAGGVFEFYGSNDRFISVATGSQLQHVTIN
                     KSGKAATTAIPEEPLYDERSGMMLGDGSRANNITLDTDVTILGNLNITAGSLTINDKT
                     LTVNNNCFIYGTLNVTHASDVFNAGTLFYNWLEFKSGSTANITDGTIMNGYPVEVNNN
                     FTVNKGEIRTAYSLVKILGNLEINNGGNLTVNSGGGIAMGASRSITVNNGGKIELNGV
                     LSNPALVTRISSGNYAFNIESGGTIAAEYGLFEHMNSGGINIKSGAIVDPDHSFNNST
                     FRNGASGGRLLTVNNNQNFTVNNAIFPSNTWGGTYNVYKNINSGIVTFSNATGAFSGS
                     AHEYDPYNRIFWGDEPLVHTIQVPAGWSGLSSYIMPLNTDITEIFSPVFSDFIMAQTM
                     SSAYYPAGGINTIVNWQSQSAYKVKMVQEAFLPISGDEETNKTLALSVGWSLVPVICN
                     TPVSASSIFSGSGFTLAKDVAGVGVYWPSMGINTLGNLLPGRAYYAYCSSPKNITFPP
                     NSKQSWAGYYPTIPVINHPWNDVEVSGSSHLIAIQVESCKGLLPGDVIGVFSPDQLCF
                     GVVEISDLQNNALLIAWADDPLTAEKDGFEEMDLMNYKLYRPQSGELFDLDVEYDQDL
                     PQTGYFYGEGLSAIKTFKMTSLGSYEALASIVSLYPNPTTGIIWAKGIKDFTKIEIYN
                     SIGNCIKTFVNQSDDNLEIDLSVHSSGVYHIKFIGKESSIIKRVVKK"
     gene            2881..3630
                     /locus_tag="PK345_06100"
     CDS             2881..3630
                     /locus_tag="PK345_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HPX34621.1"
                     /translation="MKILICISSVPDTTTKIKPTGDGKAIDLANVQWIINPWDELALT
                     RVLELKEQPNSKIEKITVLTVGGRNAEPTIRKALAIGADDAIRVDTEPGDNYFTAFQI
                     AQALKNYDYDIIICGIESSDYNGSSTGSMIAEMMDLPSVSSVSKLEIESDTVKMNREI
                     SGGFEVIEMPTPMVAIVQKGIAIEPRIPSMRGIMMARSKPLQVVESADVKPLTVFCSY
                     TLPSPKAACKMVDAENMEELVNLLHNEAKII"
     gene            3652..4632
                     /locus_tag="PK345_06105"
     CDS             3652..4632
                     /locus_tag="PK345_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HPX34622.1"
                     /translation="MNKVIIFAENFEGVFKKATFEAVSYGSRIVEQMGGGEVIAISLG
                     EVADEELKSLGAYGATRVISLHNEAFNSLDAKIYAKTIADNVKKENAGLIIFTDSNAS
                     KAVAPRVAVRLHAGMVFGAYGLPSNLSPFTIPKKVFYGKAFTEVVIQSEVKVISLSQN
                     SFGVVENKKDIVVENIDHTVDTDQIKTKILSVEKSTGKILLTDAEIVVSGGRGMKGPD
                     KWKPLEDLAETLGAGLACTRPVSDEGWRPHTEHVGQTGKIIAPNLYLAFGISGAIQHL
                     AGVSSSKVIVAVNTDKDAPIFSAADYGVVGDAATVLIGLNDALKKFKSEN"
     gene            complement(4925..6001)
                     /gene="buk"
                     /locus_tag="PK345_06110"
     CDS             complement(4925..6001)
                     /gene="buk"
                     /locus_tag="PK345_06110"
                     /EC_number="2.7.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047761 - butyrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006113 - fermentation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="butyrate kinase"
                     /protein_id="HPX34623.1"
                     /translation="MEEIGILVINPGSTSTKIGVFVNLNPIFLKNIQHNPAELAEFEK
                     ISDQFDYRKEIIATQLNNANIPKDIIKAVVGRGGLLKPIESGVYLVNERMKEDLRNGS
                     VGEHASNLGGLIADAFAQQLQGVKAYIADPVVVDELDDIARISGNPKLNRISIFHALN
                     QKTVARSHAKSVMRKYEDMNLIVAHLGGGITVGAHKMGRVIDVNNGLDGDGPFSPERS
                     GTLPVGDLVRLCFSGKYTQKEVVRMVKGEGGLVGYLGTNNAYEVELRMNDGDEEARKI
                     YEAMAYQVAKEIGAISTVLKGQIDGILITGGIANSKWFVNQIIERVHWIAPIHVYPGE
                     DEMRALAFNGMRVLTGETEPKIYT"
     gene            complement(6101..6994)
                     /locus_tag="PK345_06115"
     CDS             complement(6101..6994)
                     /locus_tag="PK345_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional enoyl-CoA hydratase/phosphate
                     acetyltransferase"
                     /protein_id="HPX34624.1"
                     /translation="MRHLSEIIEIAKQKRTRKLALAAAGDEDALLAVKNAVENDLIEP
                     VLVGNMLNINIIAKNIDFDISKYETHHEEDKFKASQKAVELIREGKAEILMKGAVDTG
                     TLMKAVLDKEKGLRKGDTLSHVAIFESQYYHKLLGVTDAAMNINPEFETKIAIIKNAI
                     ELFHKLGVQTPKVAIVGPVETVNPKIESTIHAATISMMNYRKQITGCIIDGPLAIDNA
                     TSKKSAELKNITSDVAGDADILMAPNIDSANILYKTLNFLGGAVTGAVIMGAKVPIVL
                     TSRSDSDKSKFYSIALAASIG"
     gene            complement(7099..8376)
                     /locus_tag="PK345_06120"
     CDS             complement(7099..8376)
                     /locus_tag="PK345_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M64 family metallopeptidase"
                     /protein_id="HPX34625.1"
                     /translation="MRTKISLALLSLLCLTVIAKAQFEDYFHDRTLRFDYFHAGNADM
                     EYYYFDELLDEPFWGGSKVNLIDTLRYGQYFFEVTDLLSDQIIYSRGYCTLFGEWQTT
                     DEAKQTYKSFTETVVLPFPKNNVRVDFYSRNRKGIFEKKFEYIVDVNDYFIKKERRME
                     YPVYDVHLSGLPEHKVDLVLLPEGYSSSEMELFMNDCKTFADHLFSFEPYTSNKDKFN
                     IRAVLAASPESGCDIPAEDIWVKTLLDVGFYTFNSERYCMTTNNKAVRDMAANTPYDQ
                     IYILANHKKYGGGAIYNYYSLSVNSNRAAAKIFIHEFGHGFAGLGDEYYDSEVAYSDF
                     YPLDVEPWESNLTTLVDFDKKWKNLLDPETPIPTPINDEFSNVLGVFEGGGYAAKGIY
                     RPAVDCLMHTFKGNTFCQACSNAIKQMIDFYSE"
     gene            complement(8639..9038)
                     /gene="ssrA"
                     /locus_tag="PK345_06125"
     tmRNA           complement(8639..9038)
                     /gene="ssrA"
                     /locus_tag="PK345_06125"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            9151..10623
                     /locus_tag="PK345_06130"
     CDS             9151..10623
                     /locus_tag="PK345_06130"
                     /inference="COORDINATES: protein motif:HMM:NF017508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HPX34626.1"
                     /translation="MNITKEQTGELTATLKVEVKESDYNADYQQELKKYARQSVIPGF
                     RPGKVPVGMIEKKYGTAIRADSINKLLNEKLTEYIKEEKLNILGEPIPNSEKGKIDFE
                     RQKDFDFYFDICNIPEINIELNASIELDFFQIKPTDEQVNKQLEHLRVMYGKEVEVEM
                     VKPDTKVDIELRELNDEGVVVEDGIFSTCNILINQYSAAYAIERLTGKKAEDVLTVNP
                     IELFGDLKSAADKMAVDKSKLEDKPDSFELKIIKIQRRELAELNQEFFDKAFHEKGVS
                     DVDSAKQEVEKQMQEYFSNDGQFVLYNDFIQWMFDNTKINLPDDFLRKWLHSINENKP
                     SMEEIDKELVNVKNKLQTEIIFNKLKENYPQIEVNYDEVKESFKQQFKKVLKVYSNYG
                     DEEADKEVESFIDEWMRNKKNNNEIENRFQQLHQEKVLDLIRNKCTIKEIGVTFDEFN
                     NIMKERKRDTLSENDSASHHDDEEHHDHEHSEPESENDQE"
     gene            10714..11394
                     /gene="clpP"
                     /locus_tag="PK345_06135"
     CDS             10714..11394
                     /gene="clpP"
                     /locus_tag="PK345_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HPX34627.1"
                     /translation="MFSHNDFSKYALRHRGISSLTMHRYQKLVSGYISPTIIEERQLN
                     VAQMDVFSRLMMDRIIFLGVPIYDDVANIIQAQLLFLESVDSSKDIQIYINSPGGSVY
                     AGLGIYDTMQYIGSDVATICTGMAASMGAVLLCAGKEGKRTALQHSRILIHQPMGGAE
                     GQASDIEITAREIQKLKKELYEIIAKHSKKDYDTIWKDADRDFWMTSEEAKAYGMIDE
                     VLYSAKKS"
     gene            11406..>12231
                     /gene="clpX"
                     /locus_tag="PK345_06140"
     CDS             11406..>12231
                     /gene="clpX"
                     /locus_tag="PK345_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HPX34628.1"
                     /translation="MSKQQDKCSFCGRGRNEVKLLIAGLNAYICNECTLQANYILKEE
                     GAIAKSKIATDFKLQKPNEIKHYLDQYMIGQEEAKKVLSVAVYNHFKRISQKNYKKGA
                     DDDVEIEKSNIIIVGETGTGKTLMARTISKMLKVPFAIADATVLTEAGYVGEDVESVI
                     LRLLQAADYNVKDAERGIVFIDEIDKIARKSDNPSITRDVSGEGVQQALLKLLEGSIV
                     NVPPHGGRKHPEQDMIKVNTENILFIAGGAFDGIEKIIASRLRTNVIGYSTKKEKEL"
BASE COUNT         3722 a         2223 c         2533 g         3753 t
ORIGIN      
        1 ttgaacgtta agggcaattg gactaatagt atttttcctg ccggttttga tgcaggcagc
       61 agtagggtaa gatttaccgg tcctggtcat caatatgtaa caacgagcga gacttttaat
      121 attctggagg tggccaatgg cgctgcctta cgggtagatg cgtcaacttc ggaggtaact
      181 tgtaatcaat atgactggac tagtggaggg attgatgtca ttaaaggcac tttcacagct
      241 cttgatcttg ctcaaaatgg tatttatggt agttattacg taaacccagg gggtgtaatt
      301 aatcttgaaa agataaatgg ctgggttgat tttaatggtc atatctatat ttttggtggc
      361 gagttcaata tttatggagg tacaagtgat tcctattggc cttatcaggc taacgcctct
      421 cttactatgt ctggtggtac cctcgatttt aaggatgttg gaattgttgt ttataattcc
      481 aacagtttta cttttacaca cgatattacc ggcggtacaa tacgtacaat tggaggttta
      541 acagtttata ggtcagattt tactcctgca ggcggtgttt ttgagtttta cggaagtaat
      601 gacagattta tttctgttgc aactggaagc caacttcaac atgtaactat taataagagc
      661 ggcaaggctg caactactgc tattcccgag gagcctctgt atgatgaacg cagcgggatg
      721 atgcttggcg atggaagcag agcaaataat attaccctgg atactgatgt taccattctt
      781 ggcaacctta atattactgc cggttcgctg accattaacg acaaaacact cacagtaaat
      841 aataactgct ttatttatgg tactttgaat gtgacccatg cttctgatgt ttttaatgca
      901 ggaacactat tctataattg gctggaattt aaatcgggat caacggccaa tattactgat
      961 gggacaataa tgaatggata cccagtagag gtcaacaaca attttaccgt caataagggt
     1021 gaaatccgta cagcatattc ccttgttaag attttgggca atcttgaaat aaataatggc
     1081 ggaaacctta cggtaaattc cggtggcggg attgccatgg gagcatccag gtcaatcacc
     1141 gtaaacaatg gcggtaaaat tgaacttaac ggcgtactat ctaatccagc tttagttact
     1201 cgtatttcgt caggtaatta cgcatttaat attgaaagcg gaggtaccat tgctgctgaa
     1261 tacggattgt ttgagcatat gaacagtggc ggaattaata taaaatccgg tgcaattgtt
     1321 gatcctgatc attcctttaa taactctact tttaggaatg gagcttctgg cggccggctt
     1381 ttgacggtta ataacaatca gaattttacg gtaaacaatg ctatattccc gagcaacaca
     1441 tggggtggta catataatgt ttacaaaaat attaactccg gcatagttac tttttctaat
     1501 gctaccggcg ctttttcggg tagcgcacat gagtatgatc cctataaccg gattttctgg
     1561 ggcgatgagc cgttggttca cactatccag gtacctgccg gctggagcgg gctatcgagt
     1621 tatattatgc ctttgaatac cgatattact gaaatttttt ctcctgtatt ttctgatttt
     1681 atcatggctc agacaatgag cagcgcctat tatcctgcag gtggtatcaa cacaattgtt
     1741 aactggcaga gtcagtcggc ctataaggta aagatggtgc aggaggcttt tctacctata
     1801 tcgggtgacg aggagaccaa caagacctta gctttatcag tcggctggag tcttgtgccg
     1861 gttatctgca atacacccgt ttctgcctca tcaatttttt caggttccgg atttactttg
     1921 gctaaagatg ttgccggagt tggtgtttac tggccctcta tgggtattaa caccttaggt
     1981 aacttgttgc ctggcagggc ttattatgcc tattgcagtt cacctaaaaa cattaccttc
     2041 cctcccaatt caaagcaaag ctgggcggga tattatccaa caatccctgt tattaatcat
     2101 ccgtggaatg atgttgaggt aagcggttca tctcatctta ttgccattca ggttgaatct
     2161 tgtaaaggtt tgttgccggg cgatgttatc ggagtatttt cgccagatca gctctgtttt
     2221 ggggttgttg agattagcga tttacaaaac aatgcacttc ttatagcttg ggctgacgat
     2281 cctttaacgg ccgagaaaga tggttttgaa gagatggatt tgatgaatta caaactctat
     2341 cgtcctcaaa gtggcgagtt gtttgatctt gatgtagaat atgaccagga tttacctcaa
     2401 acaggttatt tctatgggga gggattgtcg gcaatcaaaa cctttaaaat gacatcgttg
     2461 ggatcatacg aggctttggc aagcattgtc agcctttatc caaacccaac aaccggaata
     2521 atttgggcaa aagggataaa ggatttcact aagattgaga tttacaattc gataggcaat
     2581 tgtattaaaa cttttgtaaa ccaatcagat gataatttag agatagatct tagcgtacac
     2641 tcttcaggag tttatcacat taagttcatt ggcaaagaat cgagtataat caagcgtgtt
     2701 gtaaaaaaat agaaacattc attttttaat cataacctaa aaaatgccgg cctccgtaca
     2761 acgagcgcct gcttttttct gaattaatct ttcaaatgat acaaataagc tccttcagtc
     2821 ttttttatat ttttgccctt tatttaaagt tcaatttttg ttaaacataa acttcgttct
     2881 atgaagatat tgatttgtat tagcagtgtg cctgatacta ccactaaaat taaacctacc
     2941 ggtgatggta aggcaattga tctggccaat gttcagtgga tcatcaatcc ttgggatgag
     3001 ttggcgctta cccgtgtact cgaacttaaa gaacagccaa acagcaagat tgagaaaatt
     3061 acagtgctca cagtaggtgg aagaaatgca gagccaacca ttcgtaaagc gctggctatt
     3121 ggcgctgatg atgcaataag ggtagatact gaaccgggtg ataattattt caccgctttt
     3181 caaattgctc aggcattaaa aaattatgat tacgatatta taatttgtgg tatcgaatct
     3241 tccgattata atggatcgtc aacaggatca atgattgctg agatgatgga tttgccaagt
     3301 gtgtcgtcgg tatcaaaact tgagatcgaa tcggatacag taaagatgaa tcgggaaatt
     3361 tccggcggtt ttgaagtgat agagatgcct accccaatgg ttgctatcgt tcagaaagga
     3421 attgccattg agcctcgtat tccatctatg agaggtatta tgatggcccg cagcaaaccg
     3481 ttacaggttg tagaaagcgc tgatgttaag cctcttacag tgttttgttc ttatacattg
     3541 ccaagcccga aggcagcatg taaaatggtg gatgccgaaa atatggaaga acttgtgaac
     3601 ctgttacaca atgaagcaaa aattatttga taaaattaaa actgataaac aatgaataaa
     3661 gtaatcatat ttgctgaaaa ttttgaagga gtatttaaaa aagctacttt cgaggctgtt
     3721 tcttatggta gtcggattgt agaacagatg ggtggcggag aagtaattgc aatttctctt
     3781 ggggaagttg ctgacgaaga gttaaaaagc cttggagctt atggtgcgac aagggttatt
     3841 tccctacaca atgaagcatt taactcgctc gatgcaaaaa tctatgcaaa aactatagct
     3901 gataatgtaa agaaagagaa tgctggttta atcattttta ccgacagtaa tgcttctaaa
     3961 gctgttgccc ccagagttgc tgtacggctt catgcaggta tggtatttgg ggcttatggc
     4021 ctgccttcta acctctcgcc atttacaatt ccgaaaaaag tattttacgg aaaggctttt
     4081 accgaagttg taattcaatc ggaggtaaag gttattagcc tttcacaaaa ctcctttggt
     4141 gtagttgaga ataaaaaaga tattgttgtt gaaaatattg atcataccgt ggatactgat
     4201 caaattaaaa caaagatatt gagtgttgaa aagagtaccg gcaaaattct ccttacagat
     4261 gccgagattg tggtttctgg cggacgaggt atgaaggggc cggataaatg gaaaccgctt
     4321 gaggatttgg ccgaaacact tggagctgga cttgcctgta ctcgtcccgt ttctgacgaa
     4381 gggtggcgac cacataccga acatgttggc caaacaggga aaatcattgc accaaatctc
     4441 tatttggcat ttggaatatc gggcgctata caacatttgg ctggcgttag ctcatccaaa
     4501 gtaatcgttg cggtaaatac cgataaagat gctcctattt tttcagccgc cgattatggc
     4561 gtagtaggtg atgccgctac agtgctaata ggattaaacg acgcgttaaa aaagtttaaa
     4621 tcagagaatt aaacttattt gggggattat attgaggtaa aaaattgcga taatagtgtt
     4681 ggagttgaaa atccaatgct ttaaagcaaa taaaaagcct ttccaaaaat gatgggggaa
     4741 tttatctcac taattaacga aagtagtaga attaagaaag aagttgcaat atctgataat
     4801 attgaaaatg tttctgtaat aggcgctgag gtataaatta aagtttctct caatattcaa
     4861 tacgatcctc aaaaaaagtt ttctctataa gaattttgat aagaaagatt ggtgttttac
     4921 gagatcaggt gtaaattttt ggttcagttt cgcctgtcag cactcgcata ccattgaaag
     4981 ctagcgctct catttcatct tctccaggat atacatgtat tggagcaatc cagtgtaccc
     5041 tttcaataat ttgatttaca aaccacttgg aattagcaat acctccagta ataagaattc
     5101 catctatttg tccttttaat actgtactaa ttgcgccaat ctctttcgcc acttgatagg
     5161 ccatagcctc ataaatcttt cgtgcctctt catcgccatc attcatccta agttccacct
     5221 cataagcgtt atttgttcca agatagccta ctaatcctcc ttcgcctttt accatcctga
     5281 cgacctcttt ttgtgtatat tttcctgaaa agcagagtct cactaagtcg cccactggta
     5341 atgtgcctga acgttctggt gaaaacggac cgtcaccgtc aaggccgttg tttacatcta
     5401 ttacacgccc cattttatga gcgcctactg taatgccacc gcctaaatgg gcaacgataa
     5461 ggttcatatc ctcatatttt ctcatcacac tttttgcatg agatcgtgca acagttttct
     5521 gatttagagc atgaaatatt gatattcgat tcaatttagg atttcctgat atccgcgcaa
     5581 tatcatctaa ttcatcaaca accaccggat cagcgatata ggcttttact ccttgaagct
     5641 gttgagcaaa cgcatcggcg attaatccac caaggttaga ggcgtgttcc cctaccgaac
     5701 cgttacgcaa atcctccttc attcgctcgt taaccagata aacgcctgat tcaatgggtt
     5761 ttaataatcc gccacgacct actactgcct ttataatgtc tttaggaatg ttagcattat
     5821 tgagttgggt ggctatgatc tctttgcgat agtcaaactg atcggatatt ttttcgaatt
     5881 ctgctaattc ggctgggtta tgctgaatat ttttaaggaa aatgggattt aggttcacaa
     5941 acactccgat tttcgtggat gttgaaccag gatttataac taaaatgcca atttcttcca
     6001 ttcggtggac tttatgtctt tctggttaaa tgcttctaat agttatgctt tcgtcagaaa
     6061 tagaatatgt caaatacttt ttaaattcaa ttaaaccttt ttatccaatg cttgcagcta
     6121 atgcgataga ataaaattta ctcttgtcgc tatcgctgcg tgaagtaagt actataggta
     6181 ctttagctcc cataattact gcgccggtta ctgcaccgcc aagaaaattg agggttttgt
     6241 ataagatatt agcgctatca atgtttggag ccattaaaat atctgcatct ccggctacat
     6301 cgctggttat atttttaagc tctgctgatt ttttcgaagt ggcattgtca atggcaagcg
     6361 ggccgtcaat aatgcagccg gtaatttgtt tccgatagtt catcatcgaa attgtggcag
     6421 catgtatggt ggactctatc ttaggattca ctgtctccac tggcccaact attgcaactt
     6481 ttggcgtttg tactcctaat ttatgaaaca gttcaatagc attcttaatt attgctatct
     6541 tggtttcaaa ttcggggtta atgttcatgg ccgcatcggt aactcccaat aacttatgat
     6601 agtattgtga ttcaaaaatt gcaacatggc ttaacgtatc tcctttgcgc aatccctttt
     6661 ctttgtcaag aactgctttc atcaatgtgc cggtatcaac agctcccttc ataagaattt
     6721 cagctttacc ctccctgatc agctcaacag ctttttgtga ggctttgaat ttatcctctt
     6781 catgatgggt ttcatattta ctgatatcga aatcaatatt tttagctata atattgatat
     6841 ttaacatatt gccaactaaa acaggctcaa tcagatcgtt ctcaacagca ttttttacgg
     6901 cgagcaatgc atcttcatca ccagcagcag ccaatgccaa ttttcttgtt cttttctgtt
     6961 tagctatttc aattatttcg ctcaaatgtc gcattatcat tgactttttt taaattaatg
     7021 attattaatt tagttaacag gtcgcaaaaa taatcattta ccttgcataa aaatcaattt
     7081 gtaaggttta ttttatgatt attcactata gaaatctatc atttgtttaa tagcgtttga
     7141 gcatgcctga caaaaagtgt tgcctttaaa ggtgtgcatc aggcaatcta ctgctggcct
     7201 gtaaattccc tttgcagcat agccgccgcc ttcgaatacc cctaacacgt tactaaattc
     7261 gtcgttaata ggtgtaggta ttggtgtttc aggatccagt aaatttttcc attttttgtc
     7321 gaaatccact aatgtagtca ggttagactc ccatggttcg acgtcgagag ggtaaaagtc
     7381 gctgtaagct acttcgctat cataatattc atcacctaat ccggcgaagc catgaccaaa
     7441 ttcatggatg aaaattttag ctgctgctct gttgctattt accgataagg agtaataatt
     7501 gtaaatagcg ccaccaccat attttttgtg gtttgccaat atgtaaatct ggtcgtaggg
     7561 agtgttagct gccatatccc ttacagcttt gttgtttgtc gtcatacaat atctctcgct
     7621 attaaaagta taaaagccaa catcgagaag cgtctttacc catatatctt cagccggaat
     7681 atcgcatcct gattctggag aggcagctaa tactgcccga atgttaaact tgtccttgtt
     7741 ggaggtatat ggctcaaatg agaataagtg gtcggcaaag gttttacaat cattcataaa
     7801 aagttccatt tcgctcgaag aataaccttc ggggagtaat accagatcaa ccttatgctc
     7861 aggtagcccg gataggtgta catcataaac cggatattcc atgcgacgct ctttcttgat
     7921 aaagtagtcg ttaacatcta cgatatattc gaatttcttt tcgaaaatcc cctttctatt
     7981 acggctgtaa aaatcaaccc tgacattatt tttgggaaaa ggtaatacta cagtttccgt
     8041 aaacgatttg taagtttgtt ttgcttcatc agtagtctgc cattctccaa acagggtgca
     8101 gtaacctcgt gagtaaataa tttgatccga tagaaggtcg gttacttcaa aaaagtattg
     8161 accatatcgt aaggtgtcaa ttaaatttac ttttgatccg ccccagaatg gctcgtcgag
     8221 caattcgtca aagtagtaat attccatatc ggcattacct gcatgaaaat aatcgaatcg
     8281 cagtgtccgg tcatggaaat aatcctcgaa ttgggctttt gcaataacgg ttaaacataa
     8341 caacgacaaa agcgctaatg agatttttgt acgcatgatt acaacttttt taagttctgt
     8401 ttgcgaaatt agaaaaagtt gtgtttccgg agttttattt taatggtttt tttctttttt
     8461 aaaaaaaacc gcttagtaaa tttaggagag gcgccaaaaa aaaagttgtt cagatcgagt
     8521 atctgaaatt aatcaaaaag gcaaaaataa cgggtattta ttgattaatt ggggtttacc
     8581 ggttgctatt tccatctgtt ttatttattg tatttaccgg tttatgtgag gtgtaatatt
     8641 gtggagatgc cgggagtcga acccgggtcc aaacaagcag cattaaagct ttctacatgt
     8701 ttatttcttc gttggttttc gtgcaacaac cggcgaagaa cagcctactg ttgccttagc
     8761 ttcttttttt tcatcggcct cccgaagcct gatgctgact atccttgtat tgcttcaccc
     8821 tgatctcagg caggaacaag gcaatccgcc tgcaggatgt ctcgtcctac caccttgtgg
     8881 caggattggg catgatctac taaacttcga ttatgctgcg agagcgtagt taaaattgtt
     8941 gcctgttatg gcttgagaca agttgttaac gggctgcatg ctcattgccc gacatgctta
     9001 caaataacac tcccttgctg tcaaaaccag tcatccccat aatgtaaacg aacgttttat
     9061 gcttttagca aaacagcgct gcaaagatac aagaatttga ccaattaata gtaattttgc
     9121 tgccaatttg taaaaatcat aataactata atgaatatca caaaagagca aacaggtgaa
     9181 ctgacagcta cattgaaagt agaagtgaaa gaatcagatt ataatgctga ttatcaacaa
     9241 gagttaaaaa aatatgcacg tcagtcggtt atacctggct ttaggccagg aaaagtgccg
     9301 gtggggatga tagaaaagaa atatggcaca gccattaggg cagatagtat taataaactc
     9361 ttaaatgaaa aacttaccga atacatcaaa gaggaaaaac tcaatatcct tggtgaaccg
     9421 atccctaata gcgaaaaagg aaaaattgat tttgaaagac aaaaagattt tgacttttat
     9481 ttcgatatct gcaatattcc tgagataaat attgagctca acgctagtat tgaacttgat
     9541 ttttttcaga taaaacctac tgatgaacag gttaataaac agttggaaca cttacgagtg
     9601 atgtatggaa aagaggtaga ggtggagatg gtgaaaccgg atacaaaggt tgatattgaa
     9661 ttaagggaat tgaatgatga aggagtggtt gttgaggatg ggatattctc aacttgtaat
     9721 attctaataa atcagtactc agcagcttat gccattgagc gccttactgg gaaaaaggca
     9781 gaagatgttt tgaccgttaa tccaattgaa ctttttggag atttgaaaag cgcggccgac
     9841 aaaatggcag tagataaaag taagttagaa gataagccgg actcttttga actaaaaatt
     9901 ataaaaatcc aacgtagaga gttggcagag ttgaatcagg aattttttga taaagcattt
     9961 catgaaaaag gggtgagcga tgtggattcg gctaaacaag aggtagaaaa acaaatgcag
    10021 gaatactttt ccaatgatgg acaatttgtt ttgtataacg attttattca atggatgttc
    10081 gataatacaa aaattaacct ccctgacgat ttcttgagaa aatggctaca ctctattaac
    10141 gagaataaac ctagcatgga agaaatagat aaagagcttg tgaatgtaaa aaataaactc
    10201 caaacagaaa tcatatttaa taagctaaaa gagaattatc cgcaaattga agttaattac
    10261 gatgaggtaa aagagagttt taaacagcaa tttaaaaagg tcttaaaagt ttactctaat
    10321 tatggcgatg aagaagctga taaggaagtg gaatctttta ttgacgagtg gatgagaaac
    10381 aagaaaaaca ataatgagat tgaaaatcgt tttcagcaac ttcatcagga aaaagttctt
    10441 gatctgatca gaaataaatg cactatcaaa gagattggag ttacctttga tgagtttaat
    10501 aacattatga aagaaagaaa aagagatact ttgagcgaaa atgattctgc ttcccatcac
    10561 gatgacgaag agcatcacga tcacgagcat tctgaacctg aatctgaaaa tgatcaggaa
    10621 taattagcgt cttagtttca atcatttttt ttgtgtacct ttatttcatt attaaaaacc
    10681 aatattataa ctactaaaat ttaataaatt actatgtttt ctcacaatga tttcagtaaa
    10741 tatgccctca ggcatcgcgg tatcagcagt ttaaccatgc atagatatca aaagctggtg
    10801 agtggatata tttcccctac cattatcgaa gaacgacagc ttaacgtggc tcaaatggat
    10861 gttttttcaa ggcttatgat ggacaggatt atcttccttg gagttcctat ttatgatgat
    10921 gttgctaaca ttattcaagc tcagcttttg tttctcgaat cagttgactc gtcaaaagac
    10981 attcagattt atattaattc gccaggcggt tcggtatatg caggtcttgg tatttacgac
    11041 acgatgcaat acatcggttc ggatgtagct accatttgca ctggaatggc tgcatcaatg
    11101 ggcgctgttt tactttgtgc aggtaaagag gggaaacgta ctgcgctcca gcattcgcgg
    11161 atactcattc atcaacctat gggtggcgcc gaaggacagg catctgacat cgagattact
    11221 gcacgcgaaa ttcaaaagtt aaaaaaggag ctttacgaaa ttattgccaa acattccaaa
    11281 aaggattatg ataccatctg gaaagatgca gaccgtgatt tctggatgac ttctgaggag
    11341 gcaaaagctt acggtatgat tgacgaagta ttatattcgg ccaaaaagtc ttaatccgat
    11401 ccattatgtc gaaacagcag gataaatgtt cgttttgtgg aagaggccgt aatgaagtca
    11461 aacttctgat cgcagggcta aatgcataca tatgtaacga atgtacctta caggctaact
    11521 atattttaaa ggaagagggc gctatcgcta aatcgaagat agcaactgat tttaagttac
    11581 aaaaaccaaa tgagataaag cattatctcg atcagtatat gattgggcag gaagaggcta
    11641 agaaggtgct ttctgtggct gtttacaatc atttcaagcg tatttcgcaa aagaattata
    11701 aaaaaggtgc cgatgatgat gtggaaattg aaaagtcaaa cattatcatc gttggggaaa
    11761 ccggaacagg taagactctt atggcacgaa cgatatcaaa gatgctaaaa gtgccttttg
    11821 ctattgccga cgccactgtg cttaccgaag ccggatacgt aggggaggat gtggagagcg
    11881 taatattgcg tttgttgcaa gcagccgact ataacgtgaa ggatgccgaa agaggtatcg
    11941 tatttattga cgagattgac aaaattgccc gcaaaagcga caatccatcc attacccgcg
    12001 atgtgtcggg tgaaggagtg cagcaggctc tgctaaagct gttggaaggc tcaatagtga
    12061 atgtccctcc gcatggtggt cgaaaacatc ccgagcagga tatgattaag gtaaataccg
    12121 aaaatattct ttttatcgcc ggaggcgcat tcgatggaat agaaaaaatt attgcatcaa
    12181 ggttgcgaac gaacgtgatt ggatacagta ctaaaaaaga aaaagagcta g
//
LOCUS       DAOOAI010000070        11870 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_6890_length_11870_cov_4.793652, whole genome shotgun sequence.
ACCESSION   DAOOAI010000070 DAOOAI010000000
VERSION     DAOOAI010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11870)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11870)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11870
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6890_length_11870_cov_4.793652"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1090)
                     /locus_tag="PK345_06145"
     CDS             complement(<1..1090)
                     /locus_tag="PK345_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008643811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPX34629.1"
                     /translation="MLKEHPKGLLGAALSNMGERFGFYTMMAILVLFLQAKFGLSGTH
                     AGIIYSVFYFSIYALAFVGGLIADKSRKYKSTILVGLIIMAIGYVMLGFPTPTPTPNK
                     ILFLIISCFGLLTIAFGNGLFKGNLQALVGQMYDNNEYSHKRDAGFSLFYMFINVGAL
                     FAPFTAIGIRNWWLARHSYEYDANLPALCHQHVENTLESTKVFGTGVLDQAQGLAEKV
                     FGLDPVSKFQNLATEVGGQTENLTSFANDYLNVFTSGFHYAFIAAIGAMIVSLAIFLF
                     NKKHFPNVDKKVAATAASQDTEIKMDAKEIKQRLYALLAVFGVVIFFWFSFHQNGLTL
                     TMFARDYTDLSGIKINLGFVTIQGAEIFQ"
     gene            complement(1378..3213)
                     /gene="uvrC"
                     /locus_tag="PK345_06150"
     CDS             complement(1378..3213)
                     /gene="uvrC"
                     /locus_tag="PK345_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HPX34630.1"
                     /translation="MGSSEKVKERQQNIRKIKTIIRTLPDKPGVYQFLDEKDKVIYIG
                     KAKSLKKRVSSYFSKDTGQSGKTTVMVKKIAGIQHVVVDTELDALLLENNLIKKYQPR
                     YNVNLKDDKSFPWLCIKNERFPRIFSTRHVIHDGSEYYGPYASVKLMNTLLDLIRQLY
                     PLRTCKFSLSEKNIRAKKFKVCLEYHLGNCKGPCEGLQAEEDYNESIAEIREIIKGNI
                     SNVVSNLRKLMKQYADLLAFEKAQVVKEKLNMLERYQSKSLVVNPRINNVDVFSIISD
                     QESAYVNYLRIINGAIVQAHTIEMKKKLDETDSELLTLAVADLRQRFESDSQELILPF
                     NLDFNFPAIIVTIPKIGDKKQLLDLSERNAKYYKLEKRKQMELVDPERHTKRILSTMM
                     KDLRLPELPIHIECFDNSNIQGDYPVAAMVCFKNAKPSKADYRHFNINTVAGPNDYAS
                     MEEVILRRYKRIVEEQQTLPQLIIIDGGKGQLSSAVGILEKIGLRGKISIIAIAERLE
                     ELYFPNDPVPLHIDKKSETLRLIQYMRDEAHRFGITHYRKKHEKGVIKSTLTEIDGIG
                     EGYAKKLLYKFKSVKRIGEASLEELQQAIGKVKGKQVYDFFQKKN"
     gene            complement(3192..4238)
                     /gene="thiL"
                     /locus_tag="PK345_06155"
     CDS             complement(3192..4238)
                     /gene="thiL"
                     /locus_tag="PK345_06155"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007834965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HPX34631.1"
                     /translation="MTEEKDKTPKTIAELGEFGLIDLLTKNIELTQPSTVRGIGDDAA
                     VLENGDKYLLISKDILLEGIHFDMVYQPLTHLGYKAVAINLSDIYAMNGTPTHILVGI
                     GISSRYTLEAVQEIYTGIKLACKRYKVDFIGGDTSSSVQGLIISVTVLGSVDKDKVVY
                     RGVSLPNELLCVSGDLGGAYAGLLILEREKAEFTANPDMQPDLTGNEYVLERFLKPEP
                     RQDIIKIFKDLKIKPTAMIDISDGLASEIKHLCNYSDLGCAIFEDKIPIAKQTYEIAH
                     SFKMDPTVFALNGGEDYELLFTIRQEDYDKIKNIAEISVIGHMNDNDSGINLITKSGS
                     LVKIKAQGWDHLKK"
     gene            4473..5915
                     /gene="sufB"
                     /locus_tag="PK345_06160"
     CDS             4473..5915
                     /gene="sufB"
                     /locus_tag="PK345_06160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HPX34632.1"
                     /translation="MENNQIIEQVTSGEYKYGFYTDIEMDMAPKGLNEDIIRFISQKK
                     SEPDWLLEWRLKAYRHWLTMKQPNWGHLNIPPIDYNDIIYYAAPKSKKELQSLDEVDP
                     ELLATFNKLGISLEEQKRLSGVAVDAVMDSVSVKTTFSKTLEKHGIIFCSFSEAVQKH
                     PDLVRKYMGSVVPYTDNYFAALNSAVFTDGSFCYIPKGVRCPLELSTYFRINAANTGQ
                     FERTLIIGDEESYVSYMEGCTAPQRDENQLHAAVVEIVAMKDAYVKYSTVQNWYPGNK
                     QGEGGIYNLVTKRGICRGANSKISWTQVETGSAITWKYPSCILMGDNSVGEFYSVAVT
                     NNYQQADTGTKMIHLGKNTSSTIISKGISAGFSNNSYRGLVKIVRKAENSRNFTQCDS
                     LLLGDKCGAHTFPYIHDENKSSIVEHEATTSKISEDQLFYLQQRGISEESAIGLIVNG
                     YAREVLKQLPMEFAVEAQKLLSISLEGSVG"
     gene            5971..6723
                     /gene="sufC"
                     /locus_tag="PK345_06165"
     CDS             5971..6723
                     /gene="sufC"
                     /locus_tag="PK345_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004351125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HPX34633.1"
                     /translation="MLSIKNLHVSIEGREIIKGLNLEVKAGEVHAIMGPNGTGKSTLA
                     AVVAGREGIYEITKGEITYKGKRLDDLSVEERAREGIFLGFQYPVEIPGVTIVNFMRT
                     AINEIRNYKGQEQIGAAEFLKMMEEAKNLVGIHSKLTNRSVNEGFSGGEKKKNEIFQM
                     AMLKPDLAILDETDSGLDIDALKVVANGVNTLKRPDNATIVITHYQRLLDFIVPDFVH
                     VLYDGRIVKSGGKELAIELEAKGYEWIKAENL"
     gene            6726..8141
                     /gene="sufD"
                     /locus_tag="PK345_06170"
     CDS             6726..8141
                     /gene="sufD"
                     /locus_tag="PK345_06170"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HPX34634.1"
                     /translation="MEVNTNQLSLKEDLIRLFEDNSDVLLAGETTEMIMHRKKAFELF
                     RQNGFPNTKMEKWRNTDLSAAIKENYHIYFDPAFLEGIDLKTIFQCNIPSLVTDVISQ
                     LNGWFVSENGSLKKTQDGVIIGSLQAAKKHHADIVNQYLHSIANIEKNSLIALNTAFA
                     IDGTFIYVPDNVHVEIPMQMVNIIRHYRNIFIQNHNLIILGRNARLQLLQCDDSVDQQ
                     RSVTNTVTEVFIGENSSLDHYKLQNKNNNSTFINSAFFNLEKNARLLTNGITLNGGMI
                     RNDIHVTLNGEHAEANIMGVYLMDGSQHVDNQVFVDHAYPNCFSNELFKGILDDSASA
                     VFNGHILVRRDAQKTNAYQNNKNILLTDKATVNTMPFLEIYADDVKCSHGATIGQLDQ
                     NALFYLKSRGIGDDNARLLLMYAFAAEVINYIGIEPLRIRVDDMVKKRLRGELSICDQ
                     CVLHCSSKEKPVHFEIDMSKI"
     gene            complement(8229..10544)
                     /locus_tag="PK345_06175"
     CDS             complement(8229..10544)
                     /locus_tag="PK345_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HPX34635.1"
                     /translation="MKNPKKSLLLTFLLLISAKTLFCQLSVIPHPAQVEIKPGYFNLG
                     EETMIIYSNSIVYPILEETMNKWGKLMGIRLKIVNDSTANKTGNIFISLIEKYDSIIG
                     NEGYRLFISSTSVNLSSITAAGVRYVLETIGQLLETDKETILPCADIIDYPRFTYRGM
                     HLDVSRHFHPLEFIYKAIDFLVMHKMNTFHWHLNNDQGWRLEIKKYPKLTEIGAWREN
                     TNDITWRLRPYTTDHSKATYGGFYTQDEVKKVVAYAAERNITIIPEIEMPAHAMAALE
                     AYPEYSCTGKNAGIPTGNVWPITHIYCAGKEETFHFLEDVLTEVMELFPSTYIHIGGD
                     EADKANWKTCKFCQERIKREGLKNEEELQSYFTKRIEKFLNAHGRQIIGWDEILEGGI
                     SPNATVMSWRGVQGGIEAAKMGHHAIMTPGGYCYFDYYQGDPSIEPTAIGGYTTLKQV
                     YNFEPIPSELTIEEGKFILGAQANLWSEYILDYHHAEYMLFPRLSALSEVLWSPKDKR
                     DWGSFSKRMEEQYQRYERRDINYSLSAFQVKAHAEPDPVNRQLLITLNSEAYEPEIRY
                     TFNGSEPDINSTLYTEPFPISTTTQLKATVFKNGVSMKQVLTEFYDLHNAFMANLTLQ
                     KEPSPRYPGSGKYTLVDGKIGTTSFTDGLWLGFSGDDMIAIIDLYKTTKITSTELYAL
                     QTEASWIFPPQWVSFETSKDGQNFVLQETVQKPKNIGKADKNILCYSTDKSVDDIRYI
                     RVTAKNFGVCPPGHSGEGNPAWLFVSEIKIK"
     gene            10725..11615
                     /locus_tag="PK345_06180"
     CDS             10725..11615
                     /locus_tag="PK345_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPX34636.1"
                     /translation="MQPRVKGYSFALLATVAVSTVYIFSKAALNEVSLAQFGVYWFSM
                     ALLWNGLFAMRSKEHRQFRPIPAKSLKILLFLGVLEIFATGALYSAIAITPNPTIPSF
                     LRNMEYIFITLFGIFMLKESFSRVEILGVVLTFAGAFVVSYQKGATLTDYISGTSGLM
                     FVSTSLYGIRTILAKRNIHTITPTMLAINRAIFLLFSSFVFLKITGMSLMIPTSALIN
                     IAIGSFMGPFITSYSQYSALKYIEASRASFIQSTTALFVLLGAYLFFGKLPAGYQIVG
                     GTFTIIGVLLLIVKDNSNKQ"
BASE COUNT         3629 a         2145 c         2253 g         3843 t
ORIGIN      
        1 attggaaaat ttcagctcct tgtattgtta caaagcctaa attaatttta attcctgata
       61 aatctgtata atcacgggca aacattgtca gtgttaagcc gttttggtgg aaagagaacc
      121 agaagaaaat tacaacgcca aaaacagcta ataatgcata taaacgttgt ttaatttctt
      181 tagcatccat tttaatttct gtatcttggc ttgctgctgt agctgcaact tttttatcaa
      241 cattagggaa gtgcttttta ttgaataaaa agatagctaa agagacaatc atagctccga
      301 tagctgcaat aaaagcataa tgaaagcctg aagtaaacac atttagataa tcgtttgcaa
      361 agcttgttaa gttttcggtt tgtccaccaa cttctgtggc taaattttga aatttgctta
      421 cagggtctaa accaaatact ttttcagcaa gtccttgagc ttggtctaaa actcctgtgc
      481 caaaaacttt agttgattct aatgtgtttt ctacatgctg atgacaaagt gctggaagat
      541 ttgcatcgta ttcgtacgag tgtcttgcta accaccaatt tctaattcca attgcagtaa
      601 aaggggcaaa aagagcgcca acgtttataa acatgtaaaa cagcgagaat ccggcatctc
      661 ttttatgtga gtactcgtta ttgtcgtaca tctgacctac taatgcttgc aaattcccct
      721 taaaaagtcc gttaccaaaa gcaatggtta gaagaccgaa acatgaaatt atgaggaaaa
      781 gaatcttatt aggtgttggt gtcggggtag ggaaaccaag catgacatat ccgatagcca
      841 taataataag gccaaccaga atagttgatt tgtacttcct ggatttatcg gcaattagac
      901 cgccaacaaa agccagagcg taaattgaga aataaaacac agagtagata attccggcat
      961 gagtaccgct taagccaaat ttagcctgta gaaacaacac cagaattgcc atcattgtgt
     1021 aaaaaccgaa gcgctcgccc atattggaca gagcagctcc taatagtcct tttggatgtt
     1081 cttttagcat agatgtaaaa attaactatt aatatagatt tgattaaaac attacatgtt
     1141 aaaaatgtgc gcaaaaatat tcaaattact gtaataaaca ataataacgc cgccccgttg
     1201 attattcaat aattgccttt aggctgatta acatattttt cgtttgatat tgacaacaac
     1261 atcatcttaa aaatcaggtg tataacttta ttgattttgg ctggtttagt taaatggtta
     1321 ataaagaaaa ggttttccct gttgttattt taaaagagaa aaagacaaat aacactgtca
     1381 attttttttc tgaaaaaaat catagacctg tttcccttta acttttccta tagcttgctg
     1441 aagctcttca aggcttgctt caccgattcg ttttaccgat ttgaatttgt aaagcaattt
     1501 tttagcatac ccctcgccga taccatcaat ttcggttaaa gtacttttta ttactccttt
     1561 ctcatgtttt ttgcggtaat gggtgatgcc aaagcgatga gcctcatcgc gcatgtattg
     1621 tataagacgt aatgtctctg attttttatc aatgtgtagt ggaacaggat cgttagggaa
     1681 gtatagttct tcaagtcttt ctgcgatggc tataatactg attttgccgc gtagccctat
     1741 cttctcaaga attccaacgg ctgatgataa ttgccctttt ccaccatcaa taattattaa
     1801 ttgtggcagg gtttgttgtt cttcgacaat tcttttgtat cggcgaagga taacctcttc
     1861 cattgaagcg taatcattgg ggccagcaac tgtattgatg ttaaaatgac ggtaatcagc
     1921 tttgcttggt ttagcatttt taaaacagac catagcggca accggatagt cgccctgaat
     1981 gtttgaattg tcgaaacact ctatgtgtat cggaagttca ggaagccgca ggtctttcat
     2041 cattgtactg aggatacgct ttgtatgtcg ttcagggtca accaactcca tctgtttgcg
     2101 cttttccaat ttataatact tcgcattacg ttctgaaaga tcaagaagtt gctttttgtc
     2161 acctatttta ggaattgtaa ctattatggc cggaaaatta aaatcaaggt taaatggcaa
     2221 aatcagctct tgcgagtcgc tttcaaaacg ttgacgtaga tcggcaactg caagagtgag
     2281 gagttccgaa tcggtttcat ctaatttctt tttcatctct atagtatgtg cctgaactat
     2341 cgcgccgttg atgatccgta aataatttac ataggctgat tcctgatccg aaataataga
     2401 aaagacatct acattattta ttcttggatt taccacaagc gatttgcttt gatatctttc
     2461 aagcatgttc aatttttctt ttaccacttg tgctttttca aaagcaagta ggtcggcata
     2521 ttgcttcata agtttccgta aatttgaaac tacattgctg atgttaccct ttattatttc
     2581 cctgatttca gcgatagatt cattgtaatc ttcttctgcc tgaagtcctt cgcaggggcc
     2641 tttgcaattt ccaagatgat attcaaggca tactttaaat tttttggctc taatattttt
     2701 ctctgataaa ctgaacttgc aggttcgtag aggataaagc tgacgaataa ggtcgagcag
     2761 ggtattcatc aatttaactg aggcataagg gccataatat tcggaaccgt catggattac
     2821 atggcgtgta gaaaaaatac gtgggaagcg ttcgttttta atacataacc aagggaatga
     2881 tttatcgtct ttaagattga cgttgtatcg gggttgatat tttttgataa gattattttc
     2941 taaaagaagt gcatccaact ctgtgtcaac aactacatgt tgaattccgg caatcttttt
     3001 caccattaca gttgtctttc cgctctgacc ggtatcttta ctaaaataag aggaaactct
     3061 cttttttaac gatttagcct taccaatata tatgacttta tctttctcat ccaaaaactg
     3121 atatactccg ggcttgtcgg gcaatgtccg gataattgtt ttaatcttcc tgatattttg
     3181 ttgtctctct tttacttttt cagatgatcc catccttgtg cctttatttt taccagactt
     3241 ccagattttg tgatcagatt gatacctgaa tcattatcgt tcatatggcc tattacagag
     3301 atttccgcta tatttttaat tttatcataa tcctcttgcc tgatggtgaa aagcaattcg
     3361 tagtcttctc caccgttgag cgcaaatacg gttggatcca ttttaaatga atgagcaatt
     3421 tcgtaggttt gtttagcgat tgggatttta tcttcaaaaa tcgcacatcc gagatctgaa
     3481 taattacaga ggtgttttat ttcagaagca agtccgtcag aaatatcaat catcgctgtt
     3541 ggtttaatct taagatcttt gaagattttt attatatctt gtcgtggctc aggttttaaa
     3601 aaacgttcca atacatattc attacctgtt aaatcaggtt gcatatcagg atttgcagta
     3661 aactcagcct tctcccgttc taagattaac aggccggcat aggcgccgcc aagatcgccg
     3721 gaaacacata acaattcatt gggaagtgaa acaccacggt aaaccacctt gtccttatcc
     3781 acactcccta acacagtaac agatattatt aacccttgaa cgctacttga tgtatcgcct
     3841 ccaataaaat ctactttgta tcttttacaa gccaatttta ttcctgtata aatctcttgc
     3901 acagcctcga gtgtgtaacg acttgaaatg ccaatgccta ctaaaatatg agtaggcgtc
     3961 ccgttcatgg catagatatc tgaaagattt attgcaacag ctttatatcc caaatgtgtt
     4021 aaaggttgat agaccatgtc gaaatgaatt ccctccagaa gaatatcctt gcttataaga
     4081 aggtatttgt cgccattttc taatacagca gcatcatcac caatacctct tacagtcgaa
     4141 ggctgggtta gttcaatgtt ttttgttagt aaatcaatca aaccaaattc gcccaactca
     4201 gcaatggttt taggggtttt atctttttct tctgtcatcc cgattcaatt aaaggtaacg
     4261 aattgtaaaa gtaaaacttt cggttttatt gtaaccacga atcagtgttt ttttagtact
     4321 aagatgcatg ggataatgtt aggtgcagct catcttatct tttgtaaaga attatttatt
     4381 tgtaaaactg aacctattga attgtgatat gtttaaattc aaaactaaaa tactgaaagg
     4441 tgctattttt gtacctcaag tgtaatttta atatggaaaa taatcaaatc atcgaacaag
     4501 ttacttcggg agaatacaaa tacggttttt atactgatat tgagatggat atggcgccca
     4561 aagggctgaa cgaggatatt atccgattta tatctcaaaa aaagagtgaa ccggattggc
     4621 tgctcgaatg gcgtttaaaa gcctatcgtc actggctaac aatgaagcaa ccaaattggg
     4681 ggcacctcaa tattccgccg attgattata acgacatcat ttattatgct gctcccaaaa
     4741 gtaaaaaaga gctgcaaagt cttgatgagg ttgatcctga gctgcttgca acttttaata
     4801 aattagggat ttccctcgag gagcaaaaga ggttaagtgg tgttgccgtt gatgctgtga
     4861 tggatagtgt ttcagttaag actacttttt caaaaaccct tgaaaagcat ggtattatct
     4921 tttgttcttt cagtgaggca gtgcaaaagc accccgattt agtaagaaag tatatgggat
     4981 cggttgtgcc atataccgat aattattttg cagctctcaa ctcggctgta tttaccgatg
     5041 gctcgttttg ttatatccca aagggcgtca ggtgcccatt agaactatcc acttacttca
     5101 ggattaatgc agctaatacg ggtcagttcg aacgtaccct cattataggt gatgaggaga
     5161 gttacgtaag ctacatggag ggctgcactg cgccgcaacg tgatgagaat cagcttcatg
     5221 ctgccgttgt ggagattgtt gctatgaaag atgcttatgt taaatactca actgttcaaa
     5281 attggtaccc aggtaataaa cagggagaag gcgggattta caatttggtt accaagcgtg
     5341 gtatctgccg tggcgctaat tctaaaatat cctggactca ggtagaaaca ggttctgcaa
     5401 tcacatggaa gtatccaagt tgtattttga tgggcgataa ttctgtcggt gaattttatt
     5461 cggtagctgt taccaataat tatcaacagg ccgatacagg tactaaaatg attcatcttg
     5521 ggaaaaatac aagcagtact attatctcaa aaggaatttc ggcaggattt agtaataata
     5581 gctatcgtgg cctcgtgaaa attgtgcgta aagctgaaaa ttcaaggaac tttacacagt
     5641 gcgactcatt gttgcttggc gacaaatgcg gagctcacac ttttccgtac atacatgatg
     5701 aaaataaatc ctcaatagta gaacatgaag caacaacttc aaaaatatca gaagatcagt
     5761 tgttttattt acaacaacgt ggtattagcg aagagagcgc tatcggacta atagtaaatg
     5821 gatatgctag ggaggtatta aaacagttgc caatggaatt tgctgtcgaa gctcaaaagc
     5881 tcctttcgat aagcctcgaa ggtagtgtgg gttaataaaa ataaaattat ttgtaaatat
     5941 ttatccagaa aaggaaaaaa acaatttaaa atgctgtcaa taaagaattt gcacgtttct
     6001 atagagggaa gagaaattat taaaggatta aacctcgaag taaaagctgg tgaagtacat
     6061 gccataatgg ggccaaacgg aacaggtaaa tcaacccttg cagcagtagt cgctggtaga
     6121 gagggtattt atgaaattac aaaaggagag attacctata aaggaaaaag acttgatgat
     6181 ttgtcggtag aagagcgggc acgggaagga atattcctcg gatttcaata tcctgtagaa
     6241 atcccaggtg taaccatcgt taatttcatg cggactgcca ttaatgaaat tcgtaactat
     6301 aaggggcaag aacaaatcgg agctgctgag tttcttaaga tgatggaaga ggccaaaaat
     6361 cttgtcggta ttcattcaaa acttacaaat cgctctgtaa atgaaggctt ttcaggaggt
     6421 gaaaaaaaga aaaatgagat ttttcaaatg gccatgctta aacctgacct tgccattctc
     6481 gacgaaaccg attcgggatt ggacattgat gcattgaaag tggtagccaa tggagttaat
     6541 acccttaagc gacccgataa tgcaacaatt gtaattactc attaccagcg tttactcgat
     6601 tttattgtgc ccgattttgt tcatgtatta tatgatggac gtatagttaa gtcaggtggt
     6661 aaagaactgg ctattgaact tgaagcaaag ggttacgaat ggataaaagc agaaaacctt
     6721 taattatgga agtcaataca aatcaattga gtttaaagga ggatttaatt cgattgttcg
     6781 aagataacag tgatgttctc cttgccggcg agacaactga aatgatcatg catcgcaaaa
     6841 aagcttttga gcttttccgg caaaacggat ttccaaatac aaaaatggag aagtggcgta
     6901 ataccgatct ctcggcagcg attaaagaga attatcacat ctatttcgat ccggcatttc
     6961 ttgaaggtat tgatcttaaa acaatttttc aatgtaatat tccaagttta gttaccgatg
     7021 ttatttcaca gctcaatggc tggtttgttt cagaaaacgg atcgttaaaa aaaactcaag
     7081 atggagtaat aataggaagc ctacaagctg ctaaaaaaca ccatgccgat attgtaaacc
     7141 aatatttaca ttcaatagct aacattgaaa aaaatagtct gatagcgtta aacactgctt
     7201 ttgccataga tggcacgttc atttatgttc cggataatgt tcatgtggag attccgatgc
     7261 agatggttaa cataatacgg cattatagga atatttttat tcaaaaccat aacttgatca
     7321 ttcttggccg taatgctcgt ttgcaacttt tgcaatgtga tgattcagtt gatcagcaac
     7381 gtagtgttac taacactgtt accgaagttt ttattggtga aaattcatcc ttagaccatt
     7441 ataagcttca aaacaaaaac aataattcca ccttcatcaa ttcagctttt ttcaatctgg
     7501 aaaaaaatgc cagacttctt actaatggca tcacgctaaa tggtggaatg attcgaaacg
     7561 atatccatgt tactttgaac ggtgaacatg ctgaggccaa tataatgggt gtttacctga
     7621 tggatggttc gcaacatgtg gataatcagg tttttgttga tcatgcttac ccgaattgtt
     7681 ttagcaacga gttatttaaa ggtattttag atgatagcgc cagcgcagta ttcaacgggc
     7741 atatccttgt aaggcgagat gctcagaaga caaatgcata ccaaaacaat aagaatattc
     7801 ttcttaccga taaagctact gttaacacga tgccttttct cgaaatttat gctgatgatg
     7861 ttaaatgcag ccatggcgcc actataggac agttggatca gaatgcacta ttttatttaa
     7921 aatcaagagg catcggcgac gacaacgctc gactgctact tatgtatgct tttgccgcag
     7981 aagtgattaa ctatataggg attgaaccgc taagaatcag ggtagatgat atggtgaaaa
     8041 aacgcctccg tggcgaactg tccatttgtg atcaatgtgt tttgcattgt agttcaaagg
     8101 aaaaaccagt ccattttgaa attgatatga gtaagatttg atttgaaata agacacaaac
     8161 ctattgtaca ccgatcattt tttcaacgtg aatttgagaa ttaacaaatg ttagaggcaa
     8221 taaccgacct attttatttt aatttcgcta acaaagagcc atgcaggatt cccttctcct
     8281 gagtggccgg gcgggcaaac tccgaaattt ttagcagtaa ctcgtatata ccttatgtca
     8341 tcaactgatt tgtcagttga atagcatagt atgtttttat ccgcttttcc aatgtttttt
     8401 ggtttttgca ctgtttcttg taaaacaaag ttttgcccat cttttgaagt ttcaaaactt
     8461 acccattgag gaggaaaaat ccaagatgct tccgtttgta aagcataaag ttcagtagag
     8521 gtaatctttg ttgttttata taaatctatg atagctatca tatcatctcc ggaaaagccc
     8581 aaccagagcc catctgtaaa gctggtcgtg ccaattttcc catccactaa ggtatatttt
     8641 ccgctgccgg gatatcgtgg actaggttct ttttgtaggg ttaggttagc cataaaagca
     8701 ttatgtagat cgtagaactc agtgaggact tgtttcattg atactccatt tttaaaaacc
     8761 gttgctttaa gttgggtagt agtagaaatg ggaaacggtt cggtgtatag ggtagaatta
     8821 atatcaggct cactaccgtt aaatgtataa cgtatttctg gttcgtaagc ctcagagttt
     8881 agtgtaatca acaactgcct gtttaccgga tcaggttcag catgtgcttt tacttgaaaa
     8941 gcgcttaagg aatagttgat atctcgtctt tcatatcttt gatattgctc ttccattctt
     9001 tttgaaaaac taccccagtc tcttttatcc tttggtgacc agaggacttc tgaaagtgca
     9061 gacaggcggg ggaaaagcat atattcagca tggtgataat caagtatata ctccgaccat
     9121 aaatttgcct gggcgcctaa aataaatttc ccctcttcta tagttagctc cgaaggaata
     9181 ggctcgaagt tgtaaacttg ttttagcgtt gtataacccc caatagccgt tggttcaata
     9241 gaaggatcgc cttgataata atcaaaataa caatagcctc caggggtcat tattgcatga
     9301 tgccccatct tggctgcctc gatgcctcct tgtacaccgc gccacgacat taccgttgca
     9361 tttggtgaaa tccctccttc aaggatttca tcccacccta ttatttgacg accatgagcg
     9421 ttaagaaatt tctcaattct tttagtgaaa tagctttgaa gctcttcttc gtttttcaaa
     9481 ccctctcttt taattctttc ctgacaaaac ttgcaagtct tccaattagc tttgtcggct
     9541 tcatcaccgc caatatgaat ataggttgag ggaaagagtt ccataacttc agtaagcaca
     9601 tcttctaaaa aatgaaaggt ctcttctttg cccgcacaat agatatgtgt aatgggccaa
     9661 acattccccg tagggatacc ggcatttttt ccggtgcatg aatattcagg ataagcttcc
     9721 aaagcagcca tagcatgtgc tggcatttca atttccggga taatggtaat gtttctttca
     9781 gcggcgtagg caactacttt ttttacttca tcttgtgtat aaaatccacc gtaagtagct
     9841 ttggagtgat cggtagtgta aggacgcaat ctccatgtta tatcgttggt gttttcgcgc
     9901 caggctccta tttcagtaag ctttgggtat ttttttatct ctaatctcca gccttgatca
     9961 ttgtttaaat gccaatgaaa agtattcatt ttatgcatta caagaaaatc aatcgctttg
    10021 taaataaatt caagaggatg gaaatgacgt gaaacatcta aatgcatccc gcgatacgta
    10081 aaccgtggat agtctattat gtcggcacat ggtagtattg tctctttatc agtttcaagt
    10141 aattggccaa tggtctctaa cacataacgt actccagccg cggtaattga cgaaagatta
    10201 acagaggtag aactgataaa caatctatac ccttcattgc cgataataga atcgtacttt
    10261 tcaatcaatg aaataaagat attacccgtt ttatttgcag tactatcatt tacgattttc
    10321 aacctaattc ccatcaattt accccatttg ttcatcgttt cttccaaaat cggataaaca
    10381 atagagttac tatatattat catggtctct tcgccaaggt taaaatatcc tggttttatt
    10441 tcaacttgtg caggatgcgg tattacagat agttggcaga ataaagtttt tgctgaaata
    10501 agaagtaaaa aagttagtag gagtgatttt ttcggatttt tcatatcaaa atatttttta
    10561 gttaatcaaa atcaatgcat tgaaagccaa aatacaatta tttagcaaaa ttagaatttt
    10621 gttattgttg agcatggttc ttttttaaac ttaaaaaaga aatagtcaaa aggtattgaa
    10681 tccctttaaa catcttaatt ttgtgctcat aaaaaattat ttcgttgcaa ccaagagtta
    10741 agggatattc atttgcacta ctggccactg ttgctgtatc tactgtttat attttcagta
    10801 aggcagcatt gaatgaggta agccttgcgc agtttggtgt ttattggttt tccatggcat
    10861 tgttgtggaa tgggcttttt gctatgcgct caaaggagca tcgtcagttt aggccaatcc
    10921 cggcaaaatc cttaaaaata ttactattcc ttggtgtgct tgaaattttt gccacaggag
    10981 ctttatacag cgctattgcc attactccaa accctactat tccttcattt ttacgaaata
    11041 tggaatatat ttttattact ttatttggca ttttcatgtt aaaagagagc tttagcagag
    11101 ttgaaatatt aggtgttgta cttacatttg ccggagcttt tgtggtaagt tatcaaaagg
    11161 gggcgacttt aacagattat atttctggaa catcaggttt aatgtttgtt tcaacatcat
    11221 tgtacggcat tagaacgatc cttgcaaaaa gaaacattca tacaattaca ccaacgatgc
    11281 ttgcaataaa cagagcaatt ttcctgcttt tctcttcttt tgtctttctg aaaattaccg
    11341 gcatgagttt gatgattcca acttctgcat tgattaatat tgccattggt tcttttatgg
    11401 ggccatttat aacttcttat agtcaataca gcgctcttaa atacattgag gcttcgcgtg
    11461 cctcgtttat ccagagtaca acggcccttt ttgttctttt aggagcctat ctcttttttg
    11521 gaaaactccc tgcaggctat cagattgttg gaggaacctt cacaataatt ggggttttac
    11581 tgttaatagt aaaagataat agcaataagc agtaatgaaa tttgactttt ggactttgta
    11641 atatccgagg tttaaagaaa aattgtactt tacacatctt ctgttgtcta tatccaaaaa
    11701 cctaatctaa agtaaaagac tggatgaaaa aacacccaaa acgaaaagtt tatgggtgtt
    11761 tttaaccaaa agaaaagtta cctatgaaaa ctcactcatc tgttctttga gatattaata
    11821 actttcattg tgaggttaat ttaaaactta gctgttgaat acctgattgt
//
LOCUS       DAOOAI010000071        11736 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_6986_length_11736_cov_5.121308, whole genome shotgun sequence.
ACCESSION   DAOOAI010000071 DAOOAI010000000
VERSION     DAOOAI010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11736
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6986_length_11736_cov_5.121308"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..670)
                     /locus_tag="PK345_06185"
     CDS             complement(<1..670)
                     /locus_tag="PK345_06185"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX34637.1"
                     /translation="MSLAVCLLIALFVQYEYAFEKHNPEAKNIYRLLNDFDGERDPIH
                     PLVFYGKLTDAIPELNDGVMVDLNNEDYFVVDNEQWILSGVIQTTNEFFDLFNIQMVE
                     GPQNPLTDASSAVLSRSEARKLFPDGNAVGKTIRQRNNYDFTIRGIYEDLPVTTTYRP
                     NLILNIHAKEVTDNHQYTSMKNESIHFFFRLPENADQASVAKKIREQARTAYGIDDYK
                     GTYVF"
     gene            complement(821..2071)
                     /locus_tag="PK345_06190"
     CDS             complement(821..2071)
                     /locus_tag="PK345_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002561458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HPX34638.1"
                     /translation="MDRKIEKKKGLKRKHFFWIAGGIVIVFILFRIISEAGTKSLLVD
                     RDKITISSVISGQFNDYIRISGQVEPIATIYLDAEEGGRVKERLIEEGAMVKAGEVIL
                     KLENRTLYQEIMLSESDLAQKENMLRQTRISFENQMIETRRRILQSQFEIQKKKRNFK
                     QQEALYNERLIPAETWLQAKEDFEYAVQEQLINEQKAKNDSMIMISEMNLLTDDLAKM
                     RRTLQLVYERLDNLNVKAPVDGQLGMLDAEIGQNIGQGTRIGQINVLTNYKVQAMIDE
                     HYIDRVRHGLVGSFERQEKTYHLNVRKVYPEVRQGQFKIDLVFENENPENIRTGQTYY
                     INLELGLPENAIMLPRGGFFQSTGGQWVFVLDKSGSFATKRSVRLGKQNPQYYEVIEG
                     LQPDEQVITSGYENFGDNDKLIFN"
     gene            complement(2130..3467)
                     /locus_tag="PK345_06195"
     CDS             complement(2130..3467)
                     /locus_tag="PK345_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family protein"
                     /protein_id="HPX34639.1"
                     /translation="MKYNILLSLFLLFALRAVEAQQMDLNQCIRYALDNNLAITNKNL
                     EESLNNELYMQTKRNFLPQIYGGSSANKQYGRSIDPTTNSFVNHDFFSMNFYVDTQLE
                     LFRGFSRINSMKFQKLNLLIGLEESKQKKMEIAFEVMNHYYDLLYFTRLHEIVQEQLR
                     LTILNTEKIKTMIGLGLKSESDLFEMKAQQATETHNLLLAENQYDLAAISLKSLMNWP
                     LSKELNPVDESMENTIAIQPSADRVYEAALLHMPSVWKAELDVEAAGKQLAIAKGNLA
                     PTLTLGGGIYTNFADSRKEESHNGLAEQTIPFMEQWSQNLAQSVYLNLQIPIFNRWYG
                     NSQLKQAKWRKTMAENRQEEEQQKLYRLVNEDVQQLNSLQKELDLLETKKEATQEAFL
                     IAKKKLDQGLISIIEFYTAKNQLAQAETEWIRTLLQLKIKEKTIRIYLGEELF"
     gene            3711..5069
                     /locus_tag="PK345_06200"
     CDS             3711..5069
                     /locus_tag="PK345_06200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009860650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPX34640.1"
                     /translation="MKKSRILIVEDNKSVLNALELLLQPLCEQVVTTANPNQIPSLLK
                     AHYFDAILLDMNFAAGINTGNEGFYWLQRILEHDINHSVIMITAYGDVELAVRALKEG
                     AVDFVLKPWENSKLVATINTAIQLRNSRKEVKLLKEKEQNLKAAAKKESAVIIGESPQ
                     FRQILDIVSKVAKTDANVLITGENGTGKEIIARELHRQSLRANELMVSVDMGSLSETL
                     FESELFGHVKGAFTDAKEDRAGKFEIAKGSTLFLDEIANLSLPLQAKLLAALQNREIV
                     KVGSNKKIPIDIRLVCATNSNLHSLVQKGVFREDLLYRINTIQIEIPPLRERLIDIPL
                     LANFFLNIYRAKYNKPTLEFESEVMDTMKKYSWPGNVRELQHAVEKAVILSEGTTLTR
                     RNFLFPENKLPETSAQTLEEMERQMIAASIRQHEGNLTAVAAQLGISRQTLYNKLKKY
                     RL"
     gene            5095..6450
                     /locus_tag="PK345_06205"
     CDS             5095..6450
                     /locus_tag="PK345_06205"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX34641.1"
                     /translation="MHLRILYLRLAIYILLLIVFSGLLFLSVQALNWYAVLCIIGIIW
                     VIYRIIRLFNRNPQKLSYFLNAIENQDSTLFFSEKIRHKPTKELHGSLNRMNQLIQEV
                     KLKNREQEQYYSLLLEQVATGIVVMNDSGNILLANSAARSLLNYSFLNHIEQLKRVDI
                     KLYNAFLSLQQDNQNQFVKVRNENGIKQLSLQVTSFKRHDNALRIVSVHDISNELDAK
                     EIESWQKLIRVLTHEIMNSIAPITSLSETLLNYYKLNDEATVKTASVDDQKIANTVKG
                     LEVINERGTGLIQFVESYRRLTGLSKPIRKPLVVKELIEHLLLLLENEPNFQLIQKHI
                     EISSTDLVVEADITQLSQALINLIKNAIQAVEKEENPVITIMASRSSSGYIEITVQDN
                     GPGIPTEIVDQIFIPFFTTKERGTGIGLSLTRQIMKNHGGSIEVFSLPNNTRFVLKFF
                     Q"
     gene            6737..8116
                     /locus_tag="PK345_06210"
     CDS             6737..8116
                     /locus_tag="PK345_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPX34642.1"
                     /translation="MSVQKTTKSNFIVAVISLAGAMVPFMSSAINLALPVINEKLSLN
                     ARMSGWVVTSYMLATAILQIPCARLADMVGRKRLFGLGIALFSVFSFFAGLTTSGGWL
                     IVWRSMMGLGSAMMFGTGTAILISSVSAEKRGWVLGIQTSVVYMSLAAGPFIGGILTS
                     HFGWPSIFFVAGFIGIITTIGTFIFIKDEWKEVVKSRFDYLGSILYGIGLFCLLYGFS
                     LLPKTTGIILTILGIIFLIAFTKYQSRIDSPVFNIKLFFENKVFRLSTLSALINYSAT
                     FAIAFMFSLYLQYVRGLDPKEAGLVLIVQSIIQSVTSLVSGRLSDRISASLLATIGMA
                     IVALGLLLLVFITGTTSFYFLVFILALLGLGFGFFSSPNVNVIMSSVEQKFYSMASAT
                     TGTMRLTGQAFSMGIAMMAISLVIGNVKLSPDLSLELLKSMKITFAICTLLCFAGVYT
                     SSVRLKPIK"
     gene            complement(8198..8932)
                     /locus_tag="PK345_06215"
     CDS             complement(8198..8932)
                     /locus_tag="PK345_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34643.1"
                     /translation="MLNAEEIVKNIVTTSSDKIDKEALLDKYQEPVSRFFHYHLPDGI
                     PDQQFFKIPMTGIIKLVSWTHFKSILYAHPFIGFLWQATLKMGILPIKGYDYLFKDQG
                     ATRWTFFKVIPFMSESGDEVTRSAEGRAKIESLFTPHLLIHPDIIWEAVSDEEITATW
                     KIFQETEPIHLIIGKNGDLKSAYLRRWGNPGGVKKYEYHTFGINVLKEKKYKGVIIPT
                     KGNSGWWFGTKQYDDGECFRFEVATP"
     gene            complement(9046..>9726)
                     /locus_tag="PK345_06220"
     CDS             complement(9046..>9726)
                     /locus_tag="PK345_06220"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPX34644.1"
                     /translation="IVNKSQSPVKKNYVAYSEPQVFFLEESSALQSKNYMFIKGNTIT
                     EKEKPLLNAYYEYLDGGMSSIIFQEIREYRSLAYATGASLFIPFYKDEETSLTSFVGT
                     QADKTQEAIQVMHQIITSQPEKSNRIDMVRSSLTQSINSKKPPFRQISYPVAGWIKQG
                     YDEDPRKKWVNVYETMSFDDIIQTFNNQFYKKPVITTIIGKKKRIGSEWMKEFGKITE
                     VKKKDIIK"
     assembly_gap    9727..9736
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<9737..11717)
                     /locus_tag="PK345_06225"
     CDS             complement(<9737..11717)
                     /locus_tag="PK345_06225"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HPX34645.1"
                     /translation="MFKGTDKIGTLDYVKEKIYLDSIAELYDKLSSVTAAEDKNMIQK
                     KINRLSIKSSEYAIPNETEKILGEMGCAYLNAGTSYDGIAYFNVLPSSQLQKWMMVYS
                     HRFINPVYRLFQSELEAVYEEYNMYSDNRFATALEEYLSHFFPDHPYGVPIIGYPKDL
                     KYPSMSKMHEYFETYYVANNMALILSGNFNIDEVKPMIEKYFGQWRSGKIPEAPDTWK
                     IEPFKGRVEVNKKLTPVKFGLRGYRSVPQNHPDSPLLDVIGMLLTNSAQTGLMDQLVI
                     DNKLMAAQLIGDHRADAGGEYILYIPKLIGQSLKKAENLIEEKLNKLKSGEFDNDLLE
                     AVKMEIIIGHKQNFEDQFNRGYMMIFSFMNEKEWQDILDYPETIKKVTKEDIVTIAKK
                     YFGDNYLSFHSGSGLPKKPETIKPPFENIPAKNTEEKSEFAKMIAETKVASSPPQFIE
                     FGPPGQKTKDVLIDNITDLAHLYWVNNSVNDVFNLKINYGIGTYKMPILEPASEYISL
                     VAPFSMTFADYSKELQMLGGTLNVSASEDYLTVEIIGPEPNIKPILKLIGRLLSNPSL
                     DDRKIKNLYESYRVVEKMNKKDPETLGYALYSYAVYGKESEFLKGLSAKEIKKLKAAN
                     LTYAILTALTYEADFHYSGTLDFEQVKENINIHLNP"
BASE COUNT         3232 a         2260 c         2321 g         3913 t
ORIGIN      
        1 gaaaaacgta tgttccctta tagtcatcaa tgccataggc tgttctggcc tgttccctta
       61 tttttttcgc gacagaagcc tgatcggcgt tttcgggtaa ccggaaaaag aaatgaatgg
      121 actcattttt catcgatgta tattgatgat tatccgtcac ttctttcgcg tgaatattaa
      181 gaataaggtt tggacgatag gtggtggtaa cgggcaaatc ttcgtaaata cctctgatcg
      241 taaagtcgta gttgttcctt tgacgaatgg ttttacccac tgcattgccg tcaggaaata
      301 acttcctggc ttcgcttcgt gaaagaacgg ctgaactggc atcggtgaga gggttttgag
      361 gaccttccac catctgaatg ttaaacaggt cgaaaaactc gttggtggtc tgtatcacgc
      421 cggacaatat ccactgttcg ttatccacca cgaaataatc ctcattgttc aaatccacca
      481 tcacgccatc attaagttcg gggatggcat cagtaagttt gccgtaaaaa acaagcggat
      541 gaatcgggtc acgttccccg tcaaaatcgt tcagcaagcg gtagatattt tttgcctccg
      601 gattgtgttt ttcgaaagca tattcatatt gtacaaacag tgctatcagt aagcagacgg
      661 ctaaactcaa agataacccg gtgacattga tcaccgtccc catgcgttga cgcaacaaat
      721 tccgtatggc aagctttata ttgtacattg ttaattgcta attgttaatt gcacattgcc
      781 aattgttcat tgctaattgc acattgctaa ttgttcattg ttaattgaaa atcaacttat
      841 cattatcccc aaaattctca tacccggaag taattacctg ttcatcgggt tgtagtcctt
      901 caatcacttc gtaatattgc gggttttgct ttcctaagcg gacagagcgt tttgtagcga
      961 agctgcctga cttatccaac acaaaaaccc attgcccgcc ggtactttga aagaagcctc
     1021 ctcttggcag cataatcgca ttttcgggta ggccaagttc taaattgata taataggtct
     1081 gtccggtgcg gatattttca gggttctcgt tttcaaagac cagatcaatt ttaaattgtc
     1141 cttgccgaac ctcaggatac actttgcgca cgttcaggtg ataggttttt tcctggcgct
     1201 cgaaagaacc gaccagaccg tggcgtaccc ggtcaatata atgctcgtct atcattgcct
     1261 gtaccttata attggtaagc acattgatct gaccaattcg ggttccctgg ccgatatttt
     1321 gtccgatttc tgcatccaac atccctaatt gtccgtccac tggagctttc acattaaggt
     1381 tatccaaacg ctcatacacc aattgaagcg ttcgccgcat cttagcaagg tcatccgtca
     1441 gtaagttcat ctccgatatc ataatcatgg aatcgttctt agctttttgt tcgttgataa
     1501 gctgttcctg aaccgcatat tcaaaatctt ctttggcttg cagccatgtt tcggcaggaa
     1561 taagccgttc attataaagc gcttcctgtt gtttaaaatt tcgtttcttt ttttggattt
     1621 caaactgtga ttggagtatt ctgcgacgcg tttcgatcat ctgattttca aagctgatac
     1681 gtgtttgtcg aagcatattc tctttttggg caagatccga ttcactcaac attatttcct
     1741 ggtagagagt tctgttttcg agcttgagaa ttacctcgcc ggcttttacc attgccccct
     1801 cttcaatcag acgttccttt acccgcccgc cttcctcagc atctaaataa atggttgcaa
     1861 taggctccac ctgcccgcta atacggatat agtcgttgaa ctgcccgctg ataacggaac
     1921 tgatggtgat cttatcgcga tcaaccaaga gcgattttgt gcctgcttcg gaaataattc
     1981 tgaaaaggat aaatacgatt acaatacccc ccgctatcca aaagaaatgc tttcttttta
     2041 agcctttttt tttctcaatt tttctgtcca tattttccat tgtcatcctt ctttttattt
     2101 aaaatagtag cgttctttta ttgttttcat taaaacagct cttcgccaag ataaatacga
     2161 attgtttttt cttttatctt caattgaagc agggtacgta tccactctgt ttcggcttgt
     2221 gccagctggt tttttgcagt gtaaaattct atgatactga ttagcccctg atccaatttt
     2281 tttttggcta ttagaaatgc ttcctgtgtt gcctcttttt ttgtttctaa aaggtctaac
     2341 tctttttgca gcgagttcaa ctgctgaaca tcttcgttta caagccgata tagtttctgt
     2401 tgttcttctt cctgacggtt ttctgccatt gtttttctcc actttgcctg tttcagttgt
     2461 gaattaccat accatcggtt gaaaattgga atttgcagat tcaggtaaac cgattgcgct
     2521 agattttgag accattgctc catgaaaggg atggtttgtt cggctaaacc gttgtgagat
     2581 tcttcttttc gcgaatcggc aaaattggta tagattcctc ctcccaacgt caatgttgga
     2641 gctaaatttc cttttgcgat ggcgagttgt tttccggcag cttccacatc aagttctgcc
     2701 ttccataccg atggcatatg tagcaaggct gcctcataaa ctctatctgc agaaggctgt
     2761 atagcaattg tattttccat gctctcatcc accgggttta attctttgga cagtggccag
     2821 ttcatcaggc ttttcaacga gatagcagct aaatcatact gattttcagc taagagcaaa
     2881 ttatgcgttt cggtggcttg ttgggctttc atttcaaaga gatccgattc ggattttaaa
     2941 cctaaaccga tcattgtttt aattttttct gtattaagaa ttgtaagcct aagctgctcc
     3001 tgaacgattt cgtgcaagcg tgtaaaataa agaaggtcgt aatagtggtt catcacctca
     3061 aatgcaatct ccatcttctt ttgcttgctt tcttcaaggc cgatcaacaa gtttagtttt
     3121 tgaaatttca tggaattaat ccggctaaac cctctaaaaa gctccaattg cgtatctaca
     3181 taaaaattca ttgaaaaaaa atcgtggttc acaaaactat tagtagtggg gtcaatggag
     3241 cgtccgtatt gtttattggc cgaactcccg ccataaatct gaggcagaaa attccgtttt
     3301 gtctgcatgt atagttcatt gttgagagat tcttcgaggt ttttatttgt tattgcgaga
     3361 ttgttgtcca aagcataacg gatgcattga ttcagatcca tttgctgagc ctcgaccgcc
     3421 cttaaagcaa ataacaaaaa cagacttagt aaaatgttgt acttcatgtc aatttttaat
     3481 gagcaattga gaaaatgcta ttcacaaaag aattactatt tccaattgtc aatagtagtg
     3541 ccataaattt aaaaagctaa ttatcaagaa gataagcttc ttgttatttt ttaaagcgta
     3601 aaaatattag acacccattg tcttgttttt tgacagttga taataaacga ctatttttgt
     3661 tcggtgatta tggagttaat gtcaatccat ttggacagag gaatcagttt atgaaaaaaa
     3721 gtagaatttt aatagtcgaa gacaacaaga gtgtactgaa cgcattggag cttttgttgc
     3781 agcctttatg tgagcaggtg gttacaaccg ctaatcccaa tcagattcca tcactgttaa
     3841 aggctcacta ttttgatgcc atcttgttgg atatgaattt tgctgctggt ataaatacgg
     3901 ggaacgaagg tttttactgg cttcagcgta ttttagagca cgatatcaat cattcggtga
     3961 ttatgattac cgcctatggt gatgtggaat tggcagtgcg tgctttaaaa gagggggcgg
     4021 ttgattttgt gttgaagcca tgggaaaaca gcaaattggt tgctacgatc aacacggcta
     4081 ttcagttgcg caactcccgt aaagaggtga agttgcttaa ggagaaggag caaaacctga
     4141 aagcagcagc aaaaaaggaa tcagcagtga ttattggcga gtctccccag tttaggcaaa
     4201 ttcttgatat agtatcgaaa gtagcaaaaa cggatgcaaa tgtgcttatt accggtgaga
     4261 acggaaccgg aaaagagata attgcccgtg agctgcatcg gcaatcctta cgtgcaaacg
     4321 agttgatggt gagtgtagat atgggatcac tctcagaaac actatttgag agtgagctgt
     4381 ttggccatgt taagggtgca tttaccgatg caaaagagga tcgggcaggc aagtttgaga
     4441 ttgcaaaagg aagcacactc ttcttagatg agattgccaa cctctcattg ccactacagg
     4501 caaaattgtt ggcagcgctg caaaatcgcg aaatagtaaa agttggatcg aataagaaaa
     4561 tccctattga catccggctt gtgtgcgcta ccaacagcaa cttgcattcc cttgtacaga
     4621 aaggagtgtt tagagaggat ttgctgtatc gcatcaacac cattcaaatt gagattccgc
     4681 ccttgcgtga gcgcctgata gatattccgt tgttagccaa ttttttcttg aatatttacc
     4741 gtgcaaagta caataaaccc actttggaat ttgaaagcga agtgatggat acaatgaaaa
     4801 aatattcctg gccgggcaat gtgagggaat tgcaacatgc agtagaaaaa gcggttattc
     4861 tgagtgaagg cactactctt acacggcgca atttcctttt tccggaaaac aaacttcccg
     4921 agacaagcgc acagacattg gaggagatgg aacgacagat gattgctgca agcatccgtc
     4981 agcatgaagg aaacctaact gccgtagcag ctcaattggg aattagccgg caaacgcttt
     5041 ataataaatt aaaaaagtat cggttgtaac gattcatcaa aaaacacaat catcatgcat
     5101 ctgcggattc tctatcttcg tttggcgatc tatattcttc tgctgatcgt tttctccggc
     5161 ctgctgtttt tgtctgttca agccttgaat tggtatgctg tattatgtat aataggaata
     5221 atttgggtga tttataggat tatcaggcta tttaatcgca atccccaaaa gctctcttat
     5281 tttttaaatg caattgaaaa ccaggattcc accttgtttt tctctgaaaa aatccggcac
     5341 aaaccgacaa aagaactgca tggcagtctg aatcggatga accagttgat tcaagaggtt
     5401 aaattaaaaa accgcgagca ggaacagtac tacagccttt tgttggagca agtagccacc
     5461 ggtatagtgg ttatgaatga tagtgggaat attttgttgg caaattcggc agccagaagt
     5521 ttgctcaact attcgttttt aaaccatatt gagcaattga aacgtgtgga tatcaagctt
     5581 tacaatgctt ttctcagctt acaacaagac aaccagaatc agtttgtaaa agtaagaaac
     5641 gagaatggaa tcaaacagtt atccttacag gttacctctt ttaaaaggca tgataacgct
     5701 ttgcgcattg tctcggttca tgatatcagc aatgaattag acgccaaaga gattgaatcg
     5761 tggcaaaaac tcattcgggt tcttacacat gaaattatga actctattgc tcccatcacc
     5821 tccctgagcg aaaccttgct aaattactat aaactcaacg atgaagcaac agtaaaaaca
     5881 gcctccgttg acgatcagaa aattgccaat acggttaaag gattggaagt aatcaacgag
     5941 cgaggcaccg gtttgattca gttcgtagag tcgtatcgca ggcttaccgg gctttccaaa
     6001 ccaattcgta aaccactggt agtgaaagag ctgattgagc atttgttgtt acttttagaa
     6061 aatgaaccta attttcagtt aatccaaaag catatcgaaa tatcctcaac tgatttagtt
     6121 gttgaagcgg acataaccca attgtcgcag gcattaatta acctcataaa gaatgcaata
     6181 caggctgttg aaaaggagga aaatccagtg ataactatta tggcaagtcg ttcatcaagc
     6241 ggttatattg agataactgt tcaagacaat ggcccgggca taccaacgga aatagtagat
     6301 cagattttca ttccgttttt cacaaccaaa gagcgtggca ccggtattgg gttaagtctt
     6361 acccgacaaa ttatgaaaaa ccatggtgga agtatcgaag tattctcctt gccaaataac
     6421 acccgttttg tccttaagtt ttttcaatag aaagagcgat ggccgcctgt tttacgaaag
     6481 tccttctaaa ttatgatttc attttggcat ttcaacttca acaatcagtt ttttggcaac
     6541 tttggatcta agtgtggttg gtgctaatgc tcaataccaa ggatgttttc tgttgattga
     6601 ttagggctaa agcttctcat ggacttgtat aatgatttat cgaaaaaaat ctaatacttt
     6661 taccgtttgg tttgttacaa tattgatatc tgtttaggtt tctaattgtc gaaaggtaaa
     6721 tccttgataa tattttatga gtgttcaaaa gactacaaaa tctaatttta ttgttgctgt
     6781 aatttcttta gccggcgcta tggttccctt tatgtcgtcg gctattaatc tggcgttgcc
     6841 ggttataaat gaaaaactgt cgttgaatgc tcgaatgtct ggttgggttg taacctctta
     6901 tatgcttgcc acagcaatac ttcaaattcc ttgtgccagg ctggccgaca tggtcggtag
     6961 gaagcgtttg tttggtcttg ggattgcact cttttcagtt ttttcatttt ttgcaggttt
     7021 aactacttcc ggtggctggc ttatcgtctg gaggtcaatg atgggcttag gtagcgcaat
     7081 gatgtttgga acgggcactg ctattttgat atcttcagtg tcagctgaaa agcgaggatg
     7141 ggtgttaggc atacaaactt ctgtagttta catgtcgctg gctgcaggcc cttttatcgg
     7201 tggcattcta acatcacatt ttggatggcc gagcatcttt tttgtagcgg gtttcattgg
     7261 aataattact actatcggca cctttatttt tataaaagat gaatggaagg aggtcgtcaa
     7321 atcacgtttc gattatcttg gatctattct ttatggaatt gggcttttct gtcttttata
     7381 tggattttct ttattgccaa aaacaactgg aataatactg accattttgg gaataatatt
     7441 tttaattgca tttacaaagt atcagagtcg gattgatagc cccgtattta atattaagtt
     7501 gttttttgaa aataaagttt ttaggctttc aactttgtca gcattaatca attattcggc
     7561 cacttttgcc attgctttta tgtttagctt gtatctgcaa tatgtgcgcg gccttgatcc
     7621 gaaagaggcc ggacttgtat tgattgttca gtcaattatt cagtcggtta cctcattggt
     7681 ttccggtcgt ctgtcggata gaatatccgc atcactttta gccactattg gtatggctat
     7741 agtagcatta gggcttttat tacttgtctt tattacaggg acaacaagtt tttactttct
     7801 tgtttttata ttagccttac ttggtttggg ttttgggttt ttctcatccc caaatgttaa
     7861 tgttatcatg agttcagtag aacaaaagtt ttatagtatg gcatcagcaa caacgggaac
     7921 aatgcgtctt acagggcagg cttttagtat gggtatcgct atgatggcta tatcattggt
     7981 tattggtaat gtaaaattat cgccggatct ctcattggaa ttgcttaaaa gcatgaaaat
     8041 aacctttgca atttgtacgc tcctgtgctt tgccggtgtt tatacatctt cagtacggtt
     8101 gaaacctata aaatagtttt aagcaaaaaa agattcgctt ttatactttt tctttagatt
     8161 gtttaattca cagtggtata ggttttattt taaaatctta aggcgtagcc acctcgaacc
     8221 tgaaacattc gccatcatcg tattgttttg ttccaaacca ccagccggag ttgccttttg
     8281 tagggataat aacgcctttg tacttttttt ccttcaacac attgattccg aaagtatgat
     8341 attcatactt tttaactccg cccgggttac cccagcgtcg taggtacgcc gactttaaat
     8401 cgccattttt accaataatc aaatgtatcg gttctgtttc ctgaaaaatt ttccacgttg
     8461 cagtaatttc ttcatcagaa accgcttccc aaataatatc tggatgtatg agtagatgtg
     8521 gagtaaagag cgactctatc ttcgctcttc cttcggccga gcgtgttact tcatcgcccg
     8581 actcactcat aaaaggaatg actttgaaaa aagtccaccg cgtagctccc tgatctttaa
     8641 agagataatc gtaacctttt atgggtaata ttcccatttt taatgttgct tgccataaaa
     8701 aacctataaa tggatgtgca taaagtatgg atttgaaatg agtccaactt actaatttga
     8761 taattccggt catgggtatt ttgaaaaatt gttgatctgg aataccatcc ggcagatgat
     8821 aatggaaaaa gcgagataca ggctcttggt atttatctaa cagcgcttct ttatcaattt
     8881 tgtccgatga agttgtaacg atgtttttta ctatttcttc agcgttaagc ataataagtt
     8941 ggaattttta cagatgtcag ttttgtatat agtgtagccc ttattttcag aagttaatcg
     9001 ttcattaaat aatatattga ttatcaaggg tttttatcac aattattatt ttattatatc
     9061 ctttttctta acttctgtta tttttccaaa ctccttcatc cattcgcttc cgattcgctt
     9121 cttttttcca attatcgttg ttatgacggg ttttttataa aactgattgt tgaaagtctg
     9181 tattatatca tcaaaactca ttgtttcgta aacatttacc catttttttc tggggtcctc
     9241 atcatatcct tgttttatcc atccggctac cggatatgat atttggcgga atggaggttt
     9301 cttgctgttt atagattgcg ttaacgagga acgcaccata tcaatccgat tacttttttc
     9361 gggttgtgag gtgataattt gatgcattac ttgaatagct tcttgggttt tgtcggcttg
     9421 cgtaccaaca aagcttgtca atgaggtttc ttcatcttta taaaatggta taaataaaga
     9481 ggcgcctgtg gcataggcta aagacctata ctcgcgtatc tcttgaaaaa taatacttga
     9541 catacctcca tcaaggtact cataatatgc gtttagcaag ggtttctctt tttctgttat
     9601 agtgttgcct ttgatgaaca tatagttttt gctttgcaag gctgacgatt cctcaaggaa
     9661 aaatacctgt ggttcgctgt aggcaacata attcttcttt acaggagact gacttttatt
     9721 gacaatnnnn nnnnnntggg gttcagatgg atgttaatat tttctttaac ctgttcgaaa
     9781 tcaagagtac cggaataatg aaaatcggcc tcataagtta atgctgttaa aattgcgtaa
     9841 gttaggttgg cagctttaag cttttttatc tcttttgccg agagtccctt tagaaactcc
     9901 gattctttac cgtaaacagc atacgaataa agcgcataac ctaatgtttc agggtccttt
     9961 ttattcattt tttccactac cctatatgac tcatagaggt ttttgatttt tctatcgtca
    10021 aggcttggat tgctgagaag tcttcctatt aatttcaaga taggctttat gttgggttca
    10081 gggcctatta tttctactgt taaataatct tctgaagcac ttacgttaag tgtgcctccc
    10141 aacatttgta actctttact gtaatcggca aacgtcattg aaaaaggagc aacgaggctt
    10201 atgtattcag aagcgggctc gagaataggc attttatagg tgccaatacc ataattgatt
    10261 ttaaggttga aaacatcatt cacactatta tttacccaat aaaggtgagc cagatcggtg
    10321 atgttatcta tcaatacgtc tttagtcttt tgtccgggag ggccaaactc aataaattga
    10381 ggtggtgatg atgctacttt tgtttcggca atcatttttg caaattccga tttttcttcg
    10441 gtgtttttag ctggtatatt ttcgaatggc ggcttgatag tttcaggttt ttttggtagc
    10501 cctgatccgg aatgaaatga aaggtaatta tccccaaaat acttttttgc gatggttacg
    10561 atatcttctt tagtcacttt tttaatggtc tcaggataat cgagtatatc ttgccactct
    10621 ttttcattca taaaagagaa tatcatcata taacctctat taaactgatc ttcaaaattc
    10681 tgtttatggc cgatgataat ttccattttc acggcttcca aaagatcatt atcaaactct
    10741 cctgatttga gcttgttcag tttctcctca ataagatttt cagctttctt caatgattga
    10801 ccaattagtt ttggtatgta aagaatatat tctcctccgg catcagcacg atggtcgcca
    10861 atcaattggg ctgccatcag cttattatca atcacaagtt gatccataag tccggtttga
    10921 gctgaattag taagtaacat accgatcacg tcaagcaacg gcgaatcagg atggttttga
    10981 ggtaccgacc gatagcctct aaggccaaac tttacaggag ttagtttttt gtttacctct
    11041 acccttcctt tgaatggctc tattttccat gtgtcgggcg cttcggggat tttaccgctt
    11101 cgccattggc caaaatattt ctctatcatc ggtttaacct catctatatt aaagttgccg
    11161 gagaggataa gagccatatt gttggctaca taatatgttt cgaaatattc atgcatcttc
    11221 gacatagagg gatattttaa atctttagga taaccaatta ttggaacacc atagggatga
    11281 tcgggaaaaa agtgagataa atactcctca agagcggtag caaaacgatt atcgctatac
    11341 atattatact cttcgtacac agcttctaat tccgattgaa atagcctgta aaccgggttg
    11401 ataaaacgat gtgaatagac catcatccat ttttgcagtt gacttgatgg cagcacattg
    11461 aaatatgcga taccgtcgta gcttgtgccg gcattaagat aagcgcaacc catctcacct
    11521 aatatctttt cagtctcatt cggaatggca tattccgacg acttaatcga aagtcggttg
    11581 atcttctttt gaatcatatt tttatcttca gcagcggtaa ctgatgacag cttatcataa
    11641 agctcggcga tgctgtcgag atagattttt tctttaacat aatcgagagt tccgatttta
    11701 tcggttcctt taaacatgat atgttcgaaa taatgg
//
LOCUS       DAOOAI010000072        11711 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_7007_length_11711_cov_4.727093, whole genome shotgun sequence.
ACCESSION   DAOOAI010000072 DAOOAI010000000
VERSION     DAOOAI010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11711)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11711)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11711
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7007_length_11711_cov_4.727093"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(112..684)
                     /locus_tag="PK345_06230"
     CDS             complement(112..684)
                     /locus_tag="PK345_06230"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid pRiA4b ORF-3 family protein"
                     /protein_id="HPX34646.1"
                     /translation="MKKDIYQIQLSLNGSKPRIWRRLVIASNILLADFHKVIQTAMGW
                     ENYHLHHFVVGDVFYAPKTKGDLFWDDMNDIDYKKVRLHDLLQVENDKILYEYDFGDG
                     WLHTITLEKILPPDPTFKYPICLTGKMRCPPEDSGGIEGYINLLANLQDIKSPGYDEA
                     ADWLGTEYDPNYFDKEEINTLLRSKNYGCF"
     gene            complement(741..1445)
                     /locus_tag="PK345_06235"
                     /pseudo
     CDS             complement(741..1445)
                     /locus_tag="PK345_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109943.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
     gene            complement(1919..2371)
                     /locus_tag="PK345_06240"
     CDS             complement(1919..2371)
                     /locus_tag="PK345_06240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34647.1"
                     /translation="MNMRNNNDIHDEFLKKLIRQIPDDQPSDDFVAKVMKVIPATSLA
                     NKVEKSTSLKWWQWGLIAAAIVALGCFIHFFDLLQYFIPKGLFDDFNAFLYIDKLYGI
                     VKVFFNIFDSFTIGYIPTIIIIAIGILYLVDRLIFNKLYFHIRFFIFM"
     gene            complement(2355..2924)
                     /locus_tag="PK345_06245"
     CDS             complement(2355..2924)
                     /locus_tag="PK345_06245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HPX34648.1"
                     /translation="MTYRDDNFYIRQVLQGNSAAYANLVERHKDIVFSVIKKIIHDRL
                     EAEELAQDVFISAYKSLSQFEGKSKFSTWLYRIAYNAAISKTRKPKKIFVSIDDPFIE
                     TQFISQVSEQSENHDLEKQIDTLYKIMEKLPPEENLLLDLFYKKDLRVEEISEITGLT
                     ISNVKVKLHRIRKRIYLEFKKLFENEYAK"
     gene            3053..4378
                     /gene="mgtE"
                     /locus_tag="PK345_06250"
     CDS             3053..4378
                     /gene="mgtE"
                     /locus_tag="PK345_06250"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HPX34649.1"
                     /translation="MTNHTVLSKLHPTDIAAIIESQKDNEQLISFLMLPKDLKANVFS
                     YFSPTMQEEMVQKLGSTQTAEILENMAPDDRTEILENIPDELVKESINLLSDKEKDIA
                     LTLLGYPEGCVARLMTPYYVQAKKDWTIKKTFEHIKKYGKKAETLNFVYVVDDHNKLI
                     DDIRIGKLLMSDEKHTLESILDYEYKSLITTMSKEDAIHYFDKYDRAALPVTTIDGTL
                     VGIVTFDDIMDEIEKRDTEDFQKLGGVEALELSYTETPLFTLVRKRAFWLTILFVGEM
                     FTTSALRYFESEIAAAVVLVLFIPLIISSGGNSGSQASTLIVRAMALKELTLKDWFYV
                     IRKELLSGLMLGLILGTVGLLRIVIWQTAGFYDYGDHWLQLGFTIFFSLIGVVLWGTI
                     SGSMIPLILKKAGLDPATSSAPLVATLVDVTGIIIYFSVAALLLTGRLM"
     gene            4535..5113
                     /locus_tag="PK345_06255"
     CDS             4535..5113
                     /locus_tag="PK345_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007657688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF151 domain-containing protein"
                     /protein_id="HPX34650.1"
                     /translation="MEKLKLEILGMSYSQSQSGAYALILGEVGGERRLPIIIGGFEAQ
                     AIAIELEKMKPARPLTHDLLKRVADAYGIGLNEVIINKFLDGVFHAQLICNDGKQIME
                     IDSRTSDAVALALRFNCPIYTYESIMSQAGIVVHEAGKPKTEEAAETESDDPFARFNV
                     QELEEMIQKAVELEDYEKASRIRDEIKRRKKP"
     gene            5385..6668
                     /locus_tag="PK345_06260"
     CDS             5385..6668
                     /locus_tag="PK345_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside transporter C-terminal
                     domain-containing protein"
                     /protein_id="HPX34651.1"
                     /translation="MALIRSLFGIFVLILIAWLFSSNRKAVSWRVVTIGLLIQVCLAF
                     GVLFIPAVELTFEFIGKIFVKVLDFTKIGSAFLFNDLMNIDNFGYVFALQILPTIIFF
                     SALTSVLFYLGVIQKVVWALAWLLTKALKISGAESLSVAGNIFLGQTEAPLMIKAYLP
                     KMNNSEMLLVMVGGMATVAGGVMAAYIGFLGGSDPQQRLIFAKHLLTASVMAAPGAIV
                     MSKILMPQTEEIDMKVEIGQEKIGKNFLDALSNGTTEGLKLAANVGAMLLVFFAFIAM
                     VNYILLVFGRYTNLNEVVTSLTGGQYDKVSLEFLLGYTFAPLMWVIGVNSQDITLLGQ
                     LLGEKLIASEFVGYTSLASMKEAAVFAEQKSVIMATYMLCGFANFASIGIQIGGIGGL
                     APNKRWFLSKYGIKALIGGTLASLLSATIIGTILG"
     gene            complement(6713..8209)
                     /locus_tag="PK345_06265"
     CDS             complement(6713..8209)
                     /locus_tag="PK345_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH3 auxin-responsive promoter family protein"
                     /protein_id="HPX34652.1"
                     /translation="MKKRIHQIELFIKYPFEVQIDWFKKLITTAKDTEWGKKFGYKSI
                     SSLDEYRERVPIGDYNSFKPFIDDLRKGKQNLLWPGSIKWFARSSGTSENKSKYIPVS
                     SEALEECHFNAGRDMLALYFNNHPDSRVFSGKGLVMGGSRNIAESNGDSYFIGDLSAI
                     LIKNLPFWAQLKSTPSTSIALMEEWESKIEKMAQITSSHDVTSITGVPSWMLVLIKKI
                     LDNTGKTNMLEIWPNLEVFFHGGVNFSPYSKQFKLLLPHERMTYMETYNASEGFFGLQ
                     DRTDENDMLLMLDYGIFYEFIPMNELENEHPKTILLDEVKLGENYAMLISTNGGLWRY
                     LIGDTVMFTTKNPFRIRITGRTKSFINAFGEELIIDNAEKALSIASEKCHAIITEYTA
                     APVYFDDEFSKAAHEWIIEFEKVPDNLDFFKETLDTALKSLNSDYEAKRYKNLILAPP
                     IIHVAPSGTFYKWMKKMNKLGGQHKVIRLYNSREYVDEILKLIQPSGL"
     assembly_gap    8211..8220
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            8551..9366
                     /gene="ispE"
                     /locus_tag="PK345_06270"
     CDS             8551..9366
                     /gene="ispE"
                     /locus_tag="PK345_06270"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018710866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HPX34653.1"
                     /translation="MISFPNAKINLGLSVITCRDDGFHNIETLFYPVPLFDILELVKS
                     SDIKTKFTRSGIVVASDYDSDLCMRALNLLKKDFEIPPLKIHLHKNIPPGAGLGGGSS
                     DGAFMIKMLNEYFELGLTEQQMENYAGILGSDCPFFIRNHPRFAKGKGNEFEDVNIDL
                     SGYFLLIIKPPFSISTKEAYESIKPSKSQVNLYKLSELPVSQWQGKMVNDFETVVFKK
                     HPILSEIKSNLYELGAVFALMSGSGTAIYGIFKHEINPHHHFPGFFCHSAWLT"
     gene            complement(9445..10704)
                     /gene="nqrF"
                     /locus_tag="PK345_06275"
     CDS             complement(9445..10704)
                     /gene="nqrF"
                     /locus_tag="PK345_06275"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010260107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit F"
                     /protein_id="HPX34654.1"
                     /translation="MVLATSTGGIIVISIAIFFVVIILLVALLVFAGKKLLPQGKVKI
                     TINDEKDLEVDPGGALLITLSNEGIYLPSACGGGGTCAMCKCQVLDGGGSILPTEVGY
                     FTRKEQLNHWRLACQVKVREDLKIHVEPEVFGVKKWECEVVSNHNVATFIKEFVVKLP
                     EGENLNFKSGGYIQIDVPPIEVDYGRDIFVEEAYRDEWDKFKMWDLKMKNSESIFRAY
                     SMANHPEEGNIIMLNIRIATPPWDRKTNSFMKVNPGICSSYIFSRKPGDKVTISGPYG
                     EFFIKPTDKEMMFIGGGAGMAPMRSHIFHLFQTEKTKRKTTFWYGGRSTRELFYMDDF
                     EKIQKENPNFKFHVALSEPKTEDKWTGYTGFIHQVILDNYLKTHPEPEEIEYYICGPP
                     MMNDAVLKMLDDYGVPIENIAYDDFGG"
     gene            complement(10731..11348)
                     /gene="nqrE"
                     /locus_tag="PK345_06280"
     CDS             complement(10731..11348)
                     /gene="nqrE"
                     /locus_tag="PK345_06280"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005856893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit E"
                     /protein_id="HPX34655.1"
                     /translation="MENMISIFVRAIFVDNMIFAYFLGMCSYLAVSKTVKTSVGLGIA
                     VVFVLGITVPVNYLIDNYLLKAGALTWISSSFADVDLGFLSFITFIAVIASIVQLVEM
                     VVEKYAPALYNSLGIFLPLIAVNCAVLGGSLFMQEREYANIGEATMFGLGSGVGWLLA
                     IVGIAAIREKIRYSQVPAPLRGLGITFIVAGLMGIAFMSFMGIKL"
     gene            complement(11396..>11711)
                     /locus_tag="PK345_06285"
     CDS             complement(11396..>11711)
                     /locus_tag="PK345_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005637998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPX34656.1"
                     /translation="VGLIITNCIIMGRFEAFSMSNKPWPSFLDGIGNASGYGLILIVV
                     AFFRELLGSGTIWGYPVIPQFLYDIGYKDNGLMILPPMALIVVGTIIWVQRAKVKELI
                     ED"
BASE COUNT         3516 a         2262 c         2138 g         3785 t
ORIGIN      
        1 ctgacagggt aattatcggt agtcattttt gccggaaacc tgtcaggttt tttaacgtta
       61 aaaacataac atccaagctt tacagatcct atttttaact aaggatagtt gttaaaaaca
      121 tccataattt ttacttctta gcaaagtatt tatctcctct ttatcaaaat aattgggatc
      181 gtattctgtt cctaaccagt cggctgcttc atcatatccg ggcgacttaa tatcttgtaa
      241 atttgcaagt aggtttatgt atccttctat tccaccggaa tcttcagggg ggcatctcat
      301 tttacctgta aggcatatcg gatatttaaa agtaggatct ggcggaagta ttttttctaa
      361 tgttatggta tgcaaccatc catctccaaa atcgtactca taaagaattt tatcattttc
      421 aacttgtaac agatcatgaa gcctgacctt tttataatct atgtcattca tatcatccca
      481 aaaaagatcg ccttttgtct taggggcata aaaaacatct ccaactacga aatgatgtaa
      541 atgataattt tcccatccca tagcggtttg aatcacttta tggaaatcag cgagcaatat
      601 attggacgca atcactaaac gtctccagat cctgggttta gacccattaa gtgataattg
      661 tatttgatat atatcttttt tcatcctaaa tatttatgta ataatagtta aagcggtttt
      721 gttcaattta tttcattttt ttataatttt ttaataaatg ccacctcttt tggggtaaga
      781 tgtctgaatt ttccacgagg caaatccttc tttgtcaatc ctgcaaatat caccctgtcg
      841 agctttacta catcataact taatgattca aatatcctcc tgacaatacg gtttttacct
      901 gaatgtaact caatgcctat atctttttta tcagccccgt ctcctacgta ggctattcca
      961 tctactttaa tgaaaccatc ctccaactcc agtccttctc caatcttgat catatcattt
     1021 cgtgtgagcg atttgttcaa ataaacatgg taaagttttg cgatacgact gcgggggtga
     1081 gtaagctttt tcgccagatc gccatcgttg gtaaaaagaa gaagtccggt agtattacga
     1141 tcaagccgtc caacaggata aattcgctct ttgcatgctt tttccacaag tatcattaca
     1201 gtattacgct tttccggatc atcagttgtg gtaatgtatc cttttggttt gttaagcagc
     1261 acatattgta acttctccgg tttaagagtt tgttcaccga tctgcacttt gtcccgaata
     1321 ttgatttttg taccaagctc agtaacaacg ataccattta cctttacagc gcctgcagta
     1381 ataagattat cggcctcccg acgagaacaa ataccagagt tagctataaa tttgttaagg
     1441 cgtaccaacg aaacttcaga ttttgatgat gacctatctc cggtaatctt tttttcagtt
     1501 ttttggaaag atggccttat tattttcttc gaattatcag atgtacgact ctgttctact
     1561 tcatcaaatc gtttgctgat cagcctggca gtttttttcg agcggcttag atttatcttt
     1621 ttagttgtgg atttagaaaa accagctcgt tgtttgctat ccttacgcga tctcttttct
     1681 gattcgttgt ttctcatgtt ccgaaaatgg atttgtctgc aaaattaata agactggtcg
     1741 ttaaataaaa agatatttat tggttttcta aaaaaaagac tttgccccga attaaatatt
     1801 tgtaaccagc ctaataaaaa atgtactgaa cacttctcag tcgggattga cttcatataa
     1861 tcaatagttc agcttaaatg tgctttttat tactcaattt gggataatta tttagccatt
     1921 acataaagat gaaaaaccta atgtggaagt acagtttatt aaaaatcaat ctgtcaacta
     1981 aataaagaat tccaatagca ataattatta ttgttggtat atagccaatt gtaaacgagt
     2041 cgaaaatatt aaaaaacact tttacaatac cgtagagttt gtctatatac aaaaaggcat
     2101 taaaatcatc gaaaagacct ttaggtataa aatattggag taggtcgaaa aaatgaataa
     2161 aacaccctaa agctacaatg gcagcagcta ttaaacccca ttgccaccat tttaaagaag
     2221 ttgatttttc caccttattt gctagcgaag tggctgggat gactttcata acttttgcta
     2281 caaaatcatc agaaggctga tcgtcgggaa tctggcgtat caattttttc aggaactcat
     2341 cgtgtatgtc gttattattt cgcatattca ttttcaaaca gttttttaaa ttctaaatag
     2401 atgcgttttc gtattctatg aagtttgacc ttaacattgg aaatggtcag accggttatt
     2461 tcgctgatct cctcaacacg caaatccttt ttataaaata agtctaacaa tagattttct
     2521 tccggaggta gtttttccat tattttatac aatgtatcta tttgcttttc aagatcgtgg
     2581 ttttcacttt gttcgcttac ttgactgata aattgggttt caataaaagg atcatcaatt
     2641 gacacgaaga tttttttcgg ttttcttgtt ttcgatattg ctgcattata ggcaatcctg
     2701 tacagccacg ttgaaaattt ggatttccct tcaaactgtg ataacgattt gtaggccgat
     2761 atgaaaacat cctgagccaa ctcttctgct tctaaccgat cgtgaataat tttttttatc
     2821 accgaaaaca ctatatcctt atgtctttcc acaaggttag cgtaagcagc agagttaccc
     2881 tgaagcacct gcctgatata aaaattgtca tcccgatatg tcatacgtta acatttgacg
     2941 cttaacacct tttatggtta cacattgcca aaagtatcct ttaaagttta acttgttatt
     3001 gcctatagcc ttttttaatt aattttaccg tccatttaat taggttttct aaatgactaa
     3061 tcatacggta ctttccaaat tgcatcctac cgacattgct gcgattattg aatctcaaaa
     3121 agataacgag caactgatca gttttctgat gcttccaaag gatctgaaag ctaatgtttt
     3181 tagttatttc tcgcccacta tgcaagagga gatggttcaa aagctgggat caactcaaac
     3241 tgctgagata cttgaaaata tggcgcctga cgaccgaacc gaaatactcg aaaacattcc
     3301 ggacgaattg gttaaggaga gtatcaatct attgtcggat aaggaaaaag atattgccct
     3361 tactttgctt ggataccccg aagggtgtgt agccaggctt atgacaccct attatgttca
     3421 ggcaaaaaaa gattggacga taaaaaagac ttttgaacac attaaaaaat atggaaaaaa
     3481 agctgagact ttaaatttcg tgtatgttgt ggacgaccat aataagctca ttgacgacat
     3541 acgcattggc aagttgctga tgtctgacga aaaacatact ttggaatcta tattggacta
     3601 tgagtacaaa agtttgataa ccactatgtc gaaagaagat gctattcatt atttcgacaa
     3661 atacgaccgt gccgcactgc cggttaccac tattgatggc acacttgtag gtatcgtcac
     3721 ttttgacgat attatggacg agatagaaaa acgcgacacc gaagactttc agaagcttgg
     3781 aggggtagaa gcacttgagc tttcatatac agaaacgcca ttatttacat tggttcgaaa
     3841 aagagctttc tggcttacca tactttttgt aggtgagatg tttaccacca gcgcgctaag
     3901 gtacttcgag agcgaaattg ctgctgctgt tgtgttagtg ctttttatcc ctttgataat
     3961 atcaagcggt ggaaattccg gatcacaggc ttcaacattg atagtaagag ctatggcatt
     4021 aaaagaatta acgttaaaag attggtttta tgtgatacgt aaagaactgt tatctggtct
     4081 tatgttgggt ttaattttgg gcacggtagg tctattaagg attgtgatat ggcaaactgc
     4141 agggttttat gattatggag accactggct tcagttagga tttactatct ttttttctct
     4201 tattggagta gttttatggg ggactattag cggctctatg attccactta tcttgaaaaa
     4261 agcaggttta gaccctgcta cttcttctgc tccattggta gccacacttg tggatgttac
     4321 ggggataatt atctatttta gtgtggctgc gttattgctt acaggaagat taatgtaata
     4381 ataatgacat cattgtataa tcataagttg aacaaagaga cggttagcaa gtttgtttta
     4441 atgaaaggtt ttttaaagat aaaattacca gaatgaatga tctttaacca aaaatttaat
     4501 tttgtaaaat catcagactg aaacaaaaca aagcatggaa aagcttaaac tcgaaatttt
     4561 aggcatgtct tacagccaat cgcaaagtgg agcttatgcg ttgatcctcg gagaggttgg
     4621 cggagaacgc aggctgccaa taattatcgg tggatttgaa gcacaagcta ttgccatcga
     4681 acttgaaaag atgaagcctg cacgaccatt aacccatgat ttattgaaaa gagttgccga
     4741 tgcatatggt attgggttga atgaagttat tatcaataaa ttcctcgatg gagtatttca
     4801 tgctcagttg atttgcaatg atggaaagca gattatggaa attgattcgc gtacatccga
     4861 cgccgtagct ctcgcacttc gatttaattg cccgatatat acttacgaaa gtattatgtc
     4921 gcaggctggt atcgttgtac atgaagccgg aaaaccgaaa actgaagaag cggcagaaac
     4981 cgagagtgac gatccttttg ctcgttttaa tgtgcaggaa ctggaagaga tgatacaaaa
     5041 agctgtagaa ttagaggact atgaaaaggc gtcgaggata agagacgaga tcaaacgccg
     5101 taaaaaaccg taacatgcga caatttcgtg ttttttaaca aaatcatcaa ttaatttttg
     5161 gtatgaaata ttcatttttg ttctttaccc ttttcctatt taccccattc gttctcttta
     5221 gtcaacttaa tcacgactcc ttatcaatta ccgaaacttc cgaagttatg tctaatgaac
     5281 cggaagttga agtatctgat tcgacaactc acgatgaagc ttccgtacag cctcaaccaa
     5341 gcgttaaacc ttttgtgatt agggataaaa ctagtttttc agttatggca ttgataagga
     5401 gcctgtttgg gatttttgtt ttaatcctca tagcctggct tttcagctcc aaccgcaaag
     5461 cagtatcatg gagggtggta accatcggct tgttaataca agtttgcctg gcttttgggg
     5521 tcttatttat tccggcggta gaacttactt ttgagtttat tggaaagata tttgtcaagg
     5581 ttcttgattt tactaaaatt ggaagcgctt ttctatttaa cgacctgatg aatattgata
     5641 attttggata cgtctttgct ttacaaatac tacctaccat tatctttttc agcgcattaa
     5701 ccagtgtgct tttctatctg ggggtcatcc aaaaggtagt gtgggcattg gcttggctgc
     5761 taacaaaagc attgaagatt tccggagccg aaagtctgtc ggttgcaggc aatatttttc
     5821 ttggtcaaac ggaagctcct ctgatgataa aagcttacct gccaaaaatg aacaattcgg
     5881 aaatgctttt agttatggtt ggaggcatgg ctactgtagc aggtggtgtt atggcagcat
     5941 atataggctt tcttggggga agcgaccccc aacagcgtct tatttttgct aaacatttgc
     6001 tcaccgcttc ggtaatggct gcaccgggcg ctattgtgat gtcaaaaatc ctaatgcctc
     6061 aaactgaaga aattgatatg aaggtagaga ttgggcaaga aaagatagga aaaaacttcc
     6121 ttgacgcact ctcaaatgga actaccgaag gcttgaaact ggctgccaat gtaggagcta
     6181 tgttattggt tttcttcgca tttatcgcta tggtcaacta tatcttattg gtctttggca
     6241 gatataccaa tttgaacgag gttgttacat cgctcactgg tggtcaatat gataaagtct
     6301 cactcgaatt tttgcttgga tatacatttg cgcctctgat gtgggttatc ggtgtcaatt
     6361 cgcaagatat tacccttctc gggcagttgc ttggcgaaaa gcttatcgct agcgaatttg
     6421 ttggatatac aagtcttgca tcaatgaaag aggctgctgt ttttgccgaa caaaagtcgg
     6481 tgatcatggc tacgtacatg ctttgcggat ttgctaactt tgcctctata ggtatccaga
     6541 ttggcggtat cggcgggctt gctccaaaca aacgttggtt tctttctaaa tatggaatta
     6601 aggccctcat tggaggaaca ttggcctctt tgctttcggc aacaataatt ggaactattt
     6661 tgggataaca gaaacgattt ctgtattttg taactgtgtt atttttactg gtttacagac
     6721 cggatggttg gatcaatttt aaaatctcat ctacatattc tcttgagtta taaagtcgta
     6781 ttaccttatg ttggccgcct aacttattca tctttttcat ccatttataa aatgtacctg
     6841 aaggagctac gtgtattatc ggtggggcga ggatcaaatt tttgtaacgt ttagcctcat
     6901 aatcggagtt aagcgattta agtgcggtat caagcgtttc tttgaagaaa tcaagattat
     6961 caggaacttt ttcaaattcg ataatccatt cgtgtgctgc tttagaaaat tcatcatcaa
     7021 aataaactgg ggcagcagta tattctgtaa tgatggcatg acacttctcg gaagctattg
     7081 acaaagcttt ttcggcatta tcaataatta gctcctcgcc aaaagcattt ataaaacttt
     7141 tggttctccc tgttattcgg atacgaaaag ggtttttagt agtaaacatc actgtgtcgc
     7201 caatcagata acgccaaagc ccgccatttg tagaaattaa catggcataa ttttcaccga
     7261 gttttacctc atctaatagt attgtttttg gatgttcatt ttccaattca ttcataggaa
     7321 taaactcata gaatattccg taatcaagca ttagcagcat atcgttttca tcggttctgt
     7381 cctgcaatcc aaaaaagccc tccgaagcgt tgtaagtctc catgtaagtc atccgttcat
     7441 gaggtaaaag aagcttgaac tgtttactgt aagggctgaa atttaccccg ccatggaaaa
     7501 acacttcgag gtttggccaa atctctaaca tatttgtttt tcctgtgttg tcaagaattt
     7561 ttttgataag taccaacatc catgaaggta ctccggtaat gctggtaaca tcatgagaag
     7621 aggtaatttg tgccattttt tcaatcttgc tttcccactc ttccattagc gctatcgagg
     7681 tgcttggagt actttttaac tgcgcccaga agggaaggtt cttaataaga attgcagata
     7741 ggtcgccgat aaaataggaa tcgccattac tctcggcaat attacggcta ccacccatta
     7801 caaggccttt gcccgaaaaa acacgtgaat cgggatggtt attaaaatag agagccaaca
     7861 tgtcgcgacc ggcattgaaa tgacactctt caagagcctc actgcttacc gggatatact
     7921 tgctcttatt ttcagatgtg ccggatgatc tggcaaacca ttttatagag cctggccaaa
     7981 gtaagttttg tttgcctttg cgtaaatcgt caataaaagg tttaaaggag ttgtaatcgc
     8041 ctatcggaac cctttctctg tattcatcaa gacttgaaat ggatttataa ccgaattttt
     8101 tcccccattc agtatctttt gcagtggtaa tcaacttttt aaaccaatca atctgtacct
     8161 caaacggata cttaataaaa agctcaatct ggtgtattcg cttcttgatc nnnnnnnnnn
     8221 aaagtgagtt aaacattgac taaaagaaga atttaaaaaa attggtctta caaaattaat
     8281 actatttacc tttcagccgt atccaactac aaaaacacta tgaaacctgt tataaaccct
     8341 attagatgaa aagctttgat aaaaaactga actcatttat gcataaaaaa gcttcaaata
     8401 attgcatttt ttccagatgt tttttgtgga aaatataaaa tgacaatact tagccatcgg
     8461 gttaagtaaa tccaaaatcg tttttttgcg ataaagagtt aaatgaaaat ctgattctgc
     8521 ttaactttgc gcttttattt tgataaaaat atgatatcat tcccaaacgc taaaattaac
     8581 cttggtcttt ctgttattac atgtcgtgat gatggatttc ataatattga aacactcttt
     8641 tatcctgtgc ctttgtttga tatactcgag ctggtaaaat catcggacat aaaaactaaa
     8701 tttacccgtt cggggattgt agttgcctcc gattacgata gtgatctttg tatgagagcg
     8761 ttgaaccttt tgaaaaaaga ttttgagatt cctcctctaa aaatccatct ccacaaaaat
     8821 atcccgccag gcgccgggct tggcggtgga tcgtccgatg gcgcttttat gatcaaaatg
     8881 ctgaacgagt actttgaact tgggcttaca gaacaacaga tggagaatta tgccggaatc
     8941 cttggaagcg attgcccatt ttttattcgt aaccatcccc gatttgcaaa aggaaaaggt
     9001 aacgaatttg aagatgtgaa cattgacctt tccggttact ttttactgat aataaaaccg
     9061 ccatttagta taagtaccaa ggaggcttac gaaagtataa aaccttcaaa atcacaagtt
     9121 aacctatata agctaagtga gttgccagta agccaatggc aaggaaaaat ggtaaatgat
     9181 tttgaaactg tagtgtttaa aaaacaccct atactttctg agataaagtc gaatctatat
     9241 gagcttggag ctgtttttgc cttaatgagt ggtagcggaa cggcaattta tggcattttt
     9301 aaacatgaaa taaatcccca tcatcatttc cctggctttt tctgccatag cgcatggttg
     9361 acctgaaaga agttattaaa caaaaaaccc gatgagtaac tattttttca tcgggtgtat
     9421 ttggattttt aacggattgg ttttttatcc tccgaaatca tcataggcaa tgttttctat
     9481 tggaacacca taatcgtcga gcatttttaa aactgcgtca ttcatcattg gcgggccaca
     9541 aatataatat tctatctctt ctggttcagg atgggtttta agataattat caagtattac
     9601 ctgatgtatg aagccagtgt atccggtcca tttatcttcg gtttttggtt ccgataatgc
     9661 gacatgaaat ttaaaattcg gattctcttt ttggatcttt tcaaagtcgt ccatataaaa
     9721 aagttctctc gttgaacggc caccatacca gaaagtggtt ttcctctttg ttttttcagt
     9781 ttggaacagg tgaaatatat gcgacctcat tggtgccatt cctgctccac cacctataaa
     9841 catcatctcc ttgtcggtag gtttaatgaa aaattcacca taaggccctg agatagtaac
     9901 cttatcgccc ggcttacgtg aaaagatata agatgaacat attcccgggt ttactttcat
     9961 aaatgaatta gtctttctat cccaaggcgg tgttgcaata cgaatattta acattatgat
    10021 gttgccttct tcagggtgat tagccattga gtaggctcgg aaaatagact cgctattttt
    10081 cattttcaga tcccacattt tgaacttatc ccactcatcc ctataggctt cttcaacaaa
    10141 aatatctcta ccgtaatcca cttcaattgg gggaacatca atctgtatat atcctcccga
    10201 tttgaaatta agattctcgc cttcgggcag tttaaccaca aattctttaa taaatgtagc
    10261 aacattatga ttggaaacca cttcacattc ccattttttc actccgaaca cttccggttc
    10321 aacatggatt ttcaagtcct ctcttacctt tacctgacaa gcaagcctcc agtggttcaa
    10381 ctgctctttt cttgtaaaat agcctacttc tgttggtaat attgatccac caccatcgag
    10441 tacctgacat ttgcacatgg cacaagtgcc gccaccacca caggcagaag gtagatatat
    10501 cccttcgttt gacagtgtta tgagtaacgc cccccccgga tctacttcaa ggtccttctc
    10561 gtcattgata gttattttta cttttccctg tggaagaagt ttcttgccgg caaaaaccaa
    10621 taaggctacc aacaatatta ttaccacaaa aaatattgca atacttatta ctataattcc
    10681 tccagtactt gtagctaaaa ccatttttta aaatttaata gtgttttcaa ttacagttta
    10741 attcccataa agctcataaa agctataccc attaacccgg ctacaataaa tgtaattcca
    10801 agccctctga gtggcgccgg cacctgacta tatctgattt tttcccttat agccgcaatt
    10861 cctacaatag ccaaaagcca cccaacacct gagccaagcc caaacatggt agcctcgcca
    10921 atatttgcgt attcgcgctc ctgcataaaa agcgatccgc cgagcacagc gcagttaacc
    10981 gcaattaaag gaagaaaaat tccaagtgaa ttgtacaagg ccggtgcata tttttctaca
    11041 accatttcaa ccaactgtac tattgaagca ataacagcaa taaaagttat aaaactcaaa
    11101 aaccccagat caacgtctgc aaatgagctt gatatccagg ttagagcgcc agcttttaac
    11161 aaataattgt caatcagata attaacaggg acggtgatgc caagtacaaa taccacggca
    11221 attccaagtc cgactgaggt cttcaccgtt tttgaaacag cgaggtatga acacatgccc
    11281 aaaaaatagg cgaagatcat attatcaaca aaaattgccc gaacgaaaat gcttatcatg
    11341 ttttccattt tatctcaatg ttttattcag gttgaacatc attttttaat catatttaat
    11401 cttcgatcag ttctttgacc tttgcacgct gtacccatat tatagtacca acaacgataa
    11461 gcgccatcgg cggaaggatc attaatccat tgtctttgta acctatatca tacagaaatt
    11521 gtggtataac aggatatccc cagattgtac cagaaccaag cagttctctg aaaaatgcaa
    11581 caacaatcag tattagccca tatcccgacg catttccaat accatcaaga aatgagggcc
    11641 aaggtttatt gctcatcgaa aaagcttcaa acctacccat gatgatacag tttgtaataa
    11701 ttaatcccac a
//
LOCUS       DAOOAI010000073        11701 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_7017_length_11701_cov_4.323029, whole genome shotgun sequence.
ACCESSION   DAOOAI010000073 DAOOAI010000000
VERSION     DAOOAI010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11701)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11701)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11701
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7017_length_11701_cov_4.323029"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1295)
                     /locus_tag="PK345_06290"
     CDS             complement(<1..1295)
                     /locus_tag="PK345_06290"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HPX34657.1"
                     /translation="MKLRFSLFLPVLVFITTELSSQNNTLTYHWDSSYEFQDFEIEIN
                     HLKADLTINPYDTLVAGEAEFSFKILREHIDSLVFNANELNIEWITIDGHKAEFRMYG
                     NRVFIYPDRDLEWQTSHKILFKYSSKPSEGLYFIGWNDPQQIKRKQIWAHRPNNWLPY
                     APAILSMEMAFTVPDDLKVFANGVRQDVVTNKNNTKTWYYQMKRPHPFFSTCLVIGDY
                     QFKSMKTQRGLPLELWYYPDWEDHFEATYKYQVDMFDFFEKEFGFEYPYELYRQAPVI
                     DYMYGAMETTTATVFGDYLMVDEHGFLGRNFVNVNAHELAHQWFGNYVSHLKLKDVWL
                     TESFATYWAKKFEQSVFGEDYYQWERHKELENTYKAARLNNYPIGHSCGGRERWYSKG
                     SLVLDMLRDILGDEEFGATMKFYLEEHPYQIAETNDLLQA"
     gene            complement(1548..3347)
                     /gene="argS"
                     /locus_tag="PK345_06295"
     CDS             complement(1548..3347)
                     /gene="argS"
                     /locus_tag="PK345_06295"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HPX34658.1"
                     /translation="MIVQLITENIIKAISLLWGQTVDSDIISIQKTNKEFEGDFTVVV
                     FPLLKVSHTSPEATAKQLGEFLVKHVGEISRFNVIKGFLNLVIDESYWLGFFTDNYDN
                     HFFGCTKKKNSKPILVEYSSPNTNKPLHLGHVRNILLGYSVSRILEANGEKVVEVNLV
                     NDRGIHICKSMLAWSKWGNGEQPASTGEKGDHMVGRYYVMFEKNYNAEVSKLIETGMD
                     REEAEKEAASIKEAQDLLVKWETGDSETRKIWNLMNGWVYQGFDETYNRLGVDFDKIY
                     YESDTYLLGRKLVVEGLKSNVFYQKDDGSVWCDMASVGLDEKLLLRADGTSVYITQDI
                     GTAKLRKDEYDPAKMIYVVGNEQNYHFDILKKILKILGMEWWDIVLHLSYGMVELPHG
                     RMKSREGTVVDADDLLDEMYQTAKKLTEELGKTENFADDEKEALYNMIGLGALKYYIL
                     KVDPKKNMLFNPEESIDFNGNTGPFIQYTHARIRSLFRKAEESELDLKDITSIIPELK
                     LKPLTKEVNLLKILHQFPELINEAGQNYSPALVANYAYELAKMFNQFYQDTPILREED
                     RNLAVFRLKLAGFVGNVIKKAMELLGIEVPERM"
     gene            complement(3451..4506)
                     /locus_tag="PK345_06300"
     CDS             complement(3451..4506)
                     /locus_tag="PK345_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamase"
                     /protein_id="HPX34659.1"
                     /translation="MPRLITYSRSEILKRTQCRVGEEKFGERIKLYEYSDNCIQQLEK
                     QDTTFVILGIPEDIGVRANLGRPGTATAWDNFLDNFLNIQNNRFCKGEWITLLGELDF
                     SEEMVRAAQLNPKIKEQRKEMFGLVEQIDEEVVYYVSQIISSGKIPIIVGGGHNNAYG
                     NIKGFSQAKGTSVNVINFDAHTDLRPMKGRHSGNGFSYSMKEGFLESYFIFGLHENYI
                     SKGVLNEIRKIAGRVKYNTYEEICVRGEKSFEDELKIAQKHIENKHFGLEIDLDSIPM
                     IASSAITPSGFSAEETRRFLHHFASLENVGYLHLCEGAPVLGSDKNPNLIGKFISYLI
                     TDFIKANINTKTKMTVK"
     gene            complement(4744..6036)
                     /locus_tag="PK345_06305"
     CDS             complement(4744..6036)
                     /locus_tag="PK345_06305"
                     /EC_number="2.5.1.76"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014776397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteate synthase"
                     /protein_id="HPX34660.1"
                     /translation="MKHTSTQTLYKLQSIATGRTFEDTGWLLEAPGENDPTLIRAIYQ
                     KQQLDADSHNPGLYKFADWLPVNRFLVGSVAPFTYKSKGLAAYLGLHNLYITFSGYWP
                     EKGHLMPTCSFKETEAYSVCARLEPDNKKILVVASAGNTARAFAKVCSDNNIPLLLCV
                     PEDYIDSLWFNHPIKENVKLISSRSSGDYYDAIHLSSIVARLDGFVAEGGAKNVARRD
                     GMGTTVLSAVTTIGAIPDYYFQAVGSGTGAIAAWEANIRLIADGRFGDNKMKLMVSQN
                     EPFTPIYDAWKSKSRKILDFDENEARLKVEIIDAKVLSNRKPPYSIIGGLYDALLDTD
                     GDVLKADNQSAREAAALFEKHEGIDIHPAAAIATATLIAEVKDKKLDKEAVIMLNITG
                     GGEKRFKNENELFYLKPEIIFDLNPNKNYVEEKVKRLF"
     gene            complement(6140..7597)
                     /locus_tag="PK345_06310"
     CDS             complement(6140..7597)
                     /locus_tag="PK345_06310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPX34661.1"
                     /translation="MNRISRVLKAFPKNFWTANTMELLERWAWYGMFMLFALYLTGSK
                     DSGALGFSQEQKGYLMGPVVAILYFLPVITGALADRYGYRKILIIAYIILASGYFMMS
                     FIKNYTSLYIVFIYVAVGAALFKPVISATIAKTTNSETSSIGFGIFYMMVNIGAFIGP
                     VVASKLRGIDWQYVFITSSVVSLVNLFIVLFFFKEPLIEKSTIPLFKSIITIFKNIGS
                     ALSDLRLLLFLLIITGFWAMYNQLFYTLPVFIDQWMDTSLLYQAIFRISPSFARAVGT
                     NSGTIAPEMLINLDALYIIMFQIFVSAFVMRYKALNAMISGILVNSIGLGLTFYTNNP
                     FFLFVSILIFGLGEMASSPKITEYIGRIAPEGKVALYMGASFLPMAGGNFFAGILSGN
                     VYGNMSDKITLLQKEIALRGLDVPAISDTFTQNDLLTHSAELMNMTQNQLTEYLWTTY
                     SPGNIWMVFAGIGIGTSLLLFLYDKFLLSTKGNNS"
     gene            complement(7607..9031)
                     /locus_tag="PK345_06315"
     CDS             complement(7607..9031)
                     /locus_tag="PK345_06315"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX34662.1"
                     /translation="MKSKSIFFLWIILFFRIGFVFGQSDAPDINEVIKNKTPLEQIME
                     LNELSRQYRNSDIESALNFCIKAYEISVTSDNKTGMAISLKNIGIIDYIIGRYDSALV
                     KYNKALKLFETINDQSELMKVKNNVALIYMAQADFLKAINHLREILQFYRINADTTEI
                     IRSLYNLGNCYYYTGEYLISMECYREAIILNEKMENDQIKVDLFIGIGALYEAQDLLE
                     NALVEYRTALALAKKMNNRSRQGVILTNIANVYLQQDEANSALEMLTTAYLIRESLDD
                     QKGMAYVLNLMGMAYEKLGQPDRAKELYISSLKISQTLGNMREVATTLSFMGVNLIKE
                     NNYHSAIEYIKTSIQITDSAQMLHELSENYKLMVFSYSAIGLTDSAEWYLDSYLKLKQ
                     YLQLSPEELNKFDITTKAKDDLIKKENRILIKQGEQLLFSLSLFWAIVLTLIAGLVIF
                     IFILTVLLILRPKYRKRFLLKMFK"
     gene            complement(9061..10194)
                     /locus_tag="PK345_06320"
     CDS             complement(9061..10194)
                     /locus_tag="PK345_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HPX34663.1"
                     /translation="MSKLIKNRRLKEYFIISRGQRNGMLILLVAIVITIILYFILPFI
                     IRPDFIWDAKFDSITLQFRQLSPEPTAKPLLNPFPFYPNFASKEIFIKLGLSERAAEN
                     IIKYRDAGGKFRTADDFGKIFSISNEEFNTLRPYIQIFKEKDVLEKSEKTIPAKILNP
                     FPFDPNLIDSTEMERLGLKDGQIRNIINYRSKGGKFKVKSDFGKLYSISEDDFSVLDK
                     YILLPSVDTSTSIQISEEKTPIIVDLNAADTIELKQLKGIGTVFARRIVSYREKLGGY
                     FDKSQLLEVFGMDTTRLAQISPYIEVDLQKIKQLHLNKAGYKELLAHPYLENYIVKAI
                     FEYKDKVGKFNSVDELQNISLIYPQLFQKIEHYFTVEDSYTRR"
     gene            complement(10205..11569)
                     /locus_tag="PK345_06325"
     CDS             complement(10205..11569)
                     /locus_tag="PK345_06325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-dependent transporter"
                     /protein_id="HPX34664.1"
                     /translation="MEKSVLSQRDSFSSKIGVIAAAAGSAIGLGNIWRFPCVTGENGG
                     GAFLVIYLVIMLLIGVPVMLSEFIIGRRAQRNPYGAFRLLKPNQPWYLIGILGIAAAF
                     MILAFYTTIAGWTLEYIYRSLINSFSGQSVEELKSSFDSFQANGLFPVIWQLIFMFLT
                     ALIVWRGIKDGIEKYSKILMPLLLLIIVVIAIRSITLPGSKEGLVFLFKPDFSKITWE
                     AVLKALGQVFFSLSIGMGTLITYGSYINKKDNLTSTALAVTGADTFVAIIAGMAIFPA
                     AFAFGIKPNAGPGLAFITLPNIFQQMTGGYFWALSFFLLLAVAALTSTISLLEVVVAY
                     FSEELKMSRKKATVIAAGSTSVLGVLCTLSQGSMNYLKVFKDTLFDDLDFISANILLP
                     LGGLMIVIFVGWFMGKENVRTEITNNGELKGRFFGIFMFILRIVAPVAIALVFLSSLE
                     LISF"
BASE COUNT         3715 a         2364 c         1954 g         3668 t
ORIGIN      
        1 gcctgtaaca gatcattggt ttcagctatc tggtaaggat gttcttcaag atagaatttc
       61 atggtagcac cgaactcttc atctccaaga atatctctta gcatatcgag tactaacgaa
      121 ccttttgaat accagcgctc ccgtccacca caactatggc cgattggata gttattcaat
      181 ctggcggctt tgtaagtatt ttctaattcc ttgtgacgct cccattgata gtaatcttca
      241 ccaaacactg attgttcaaa cttttttgcc caatatgttg caaaactctc agtaagccag
      301 acatctttca gttttaaatg cgacacgtaa ttaccaaacc attgatgtgc cagctcatga
      361 gcatttacat taacaaagtt tcttcctaaa aatccatgct cgtccaccat aagataatcg
      421 ccaaagacgg tagctgtagt agtttccatt gcgccgtaca tataatcaat cacaggggct
      481 tggcggtaaa gctcataagg gtattcaaag ccaaactctt tctcaaaaaa gtcaaacata
      541 tcaacttgat atttataagt ggcctcaaaa tgatcttccc aatcagggta ataccacagt
      601 tccaaaggta gcccacgttg tgttttcata cttttaaact gataatcacc aatcacaagg
      661 caagtagaga aaaacgggtg tgggcgtttc atttgataat accaggtttt agtgttgttt
      721 ttatttgtaa ccacatcctg cctgacacca ttcgcaaaaa cctttaaatc atcaggtacg
      781 gtaaatgcca tttccatcga aagaatcgca ggagcgtagg gtagccaatt attcggccta
      841 tgagcccaaa tttgttttcg ttttatttgc tgagggtcgt tccagccaat aaaatagagg
      901 ccttcggagg gtttagatga atatttgaac aagattttat gtgatgtttg ccattcgaga
      961 tcacgatcag gataaataaa aacacgatta ccatacattc taaactccgc tttgtggcca
     1021 tcaatcgtta tccattcaat attcagctca ttggcgttaa atactaacga atcaatgtgt
     1081 tcccttaaaa ttttaaaact gaattcggct tcgccggcaa caagggtatc ataaggatta
     1141 atggtgagat cggcttttaa gtgatttatt tcgatttcaa aatcctgaaa ttcataagag
     1201 gaatcccaat gataagttaa agtattgttt tgtgaactta attcagttgt gataaaaact
     1261 aaaaccggta aaaagagtga aaaacgaagt ttcatccatt aattgagtta gatgcgcaaa
     1321 aataaaaaat ttgtgctgga aaaggcataa aagttataca gccgaattta ctgataattt
     1381 attttcaatg atgtaatcga ggttttgaac caacagcgct ttagcaagac aacttctcaa
     1441 caataaatta cactccaaac gctgcaaatc ttctctttaa attttttgcc tactgatgca
     1501 gtggatacat gctttgaaaa tgacttttat tttccggccc ggatttatta catcctttcc
     1561 ggtacctcta tcccaagtaa ttccatagct tttttgatta cattaccaac gaagcccgcc
     1621 agtttaaggc gaaaaactgc caggtttcta tcttcttccc gtaaaattgg cgtatcctga
     1681 tagaactgat taaacatttt tgctaactcg taggcgtaat tggctactaa agcagggctg
     1741 taattctgtc cagcttcatt tatcaattca ggaaactggt gtaatatttt taaaagattt
     1801 acttctttag taagcggttt taatttgagc tctggaatta tagaggtgat atcttttaag
     1861 tctaattcac tttcttcggc tttacggaat aacgaacgga ttcgggcatg tgtgtactgg
     1921 ataaatgggc cggtattgcc gttgaaatca atagattctt cggggttgaa aagcatattt
     1981 ttctttggat caaccttcag gatataatat ttcaaagcgc caaggcctat catgttgtaa
     2041 agagcctctt tttcatcatc ggcaaaattt tctgttttac ccaattcctc ggtgagtttt
     2101 ttagctgttt ggtacatttc gtcaagcaga tcgtcggcat ccaccaccgt tccctctcgc
     2161 gatttcatcc tgccgtgtgg cagctccacc attccataag agagatgaag gactatatcc
     2221 caccactcca ttccaagtat tttcaaaatc tttttcaaaa tatcgaaatg gtagttttgc
     2281 tcattcccta caacatagat catttttgca ggatcgtact catcttttcg aagttttgca
     2341 gtaccaatat cctgagtaat ataaaccgaa gtgccatcgg ctctgagtag tagcttttca
     2401 tcaagaccaa cggaggccat atcacaccaa accgatccgt catctttttg ataaaaaaca
     2461 ttggatttta gcccttccac aactaacttt cgtcctaaaa gataggtgtc ggattcatag
     2521 tagattttat cgaaatcaac accaagtcta ttgtatgttt catcaaaacc ttgataaacc
     2581 caaccattca tcaaattcca gatttttctg gtttcggaat ctccggtttc ccatttcacc
     2641 aataaatcct gagcctcttt gatacttgca gcctcttttt cggcctcttc cctgtccatt
     2701 ccagtttcaa ttaatttgga aacttcggcg ttataatttt tttcaaacat aacgtaatat
     2761 ctgccaacca tatggtcgcc cttttcgcct gttgaagcgg gctgttcgcc attaccccat
     2821 ttcgaccatg ccaacattga cttacagata tgtatcccgc gatcgtttac caggtttact
     2881 tcaacaacct tttcaccatt ggcttccaaa atcctggaaa ctgaatagcc aagcaaaatg
     2941 ttgcgtacat ggcctaaatg caatggcttg ttagtattag gcgatgagta ttcgacaagg
     3001 attggtttag agtttttttt ctttgtacag ccaaaaaaat gattatcata attatcagta
     3061 aaaaagccaa gccaataact ttcatcaatc actaagttca aaaatccttt gatgacatta
     3121 aaacggctta tttctccaac atgtttaaca agaaattctc ccagttgctt tgcagtagct
     3181 tcgggtgatg tatgagagac ttttagcaat ggaaaaacaa ctacggtaaa atctccctca
     3241 aactctttgt ttgttttttg gatagaaatt atgtcagaat caacagtttg accccataaa
     3301 agcgaaatcg ctttaatgat attttcggtg ataagttgaa caatcattcc tgtaaaaaat
     3361 cgaataatat ttcagcaaaa ttagtaaaaa caatagctaa gataatttag agatcacaga
     3421 gagcaagcct acatacttat taatggtttg ctatttaacg gtcatctttg tttttgtatt
     3481 aatatttgct ttaataaaat cggttatcaa gtatgaaata aatttgccta tcaaatttgg
     3541 atttttatct gagcccaata ctggcgcacc ttcgcataaa tggaggtaac ccacattttc
     3601 caacgaagca aaatgatgca agaaccgcct tgtctcttct gctgagaagc cactgggggt
     3661 gatagcgcta ctggcaatca tcggaatact atccaagtca atttctaaac caaaatgctt
     3721 attctcaata tgtttttgag caatcttcaa ttcgtcttca aaagactttt cgcctctgac
     3781 gcaaatctct tcataggtat tgtatttcac cctcccggct atctttctga tttcattaag
     3841 cactcctttg gatatataat tttcgtgtaa tccaaaaatg aaatagctct ccaagaaacc
     3901 ctctttcatt gagtaagaaa aaccattacc actgtgtctt cctttcatgg gacgaagatc
     3961 ggtatgagca tcgaaattta tcacatttac acttgtgcct ttagcttgcg aaaagccttt
     4021 tatgttgcca tatgcgttat tatgaccacc tccaacgatg attggaattt ttcctgatga
     4081 tattatttgt gaaacataat agactacttc ttcatcaatt tgttcgacta aaccgaacat
     4141 ctcttttcgt tgttccttta ttttgggatt tagttgtgca gcacgtacca tttcttctga
     4201 aaaatcaagc tcaccaagca gggtaatcca ttctccttta caaaaacgat tgttttgaat
     4261 atttaaaaaa ttatctaaaa agttatccca tgcagtggct gttccaggtc ttcccaaatt
     4321 agctcgtact ccaatatctt caggaattcc taaaataaca aacgtggtat cctgtttttc
     4381 aagttgttga atacaattgt cgctatactc ataaagtttt attcgctctc caaacttctc
     4441 ttcccctact ctacattgag tccgctttaa tatctccgat cttgagtaag ttattaatct
     4501 tggcataaat taaatagttt cttatttttt atctgttatt caaaatatct tctatctatt
     4561 ttacatcttc atcagagatt tgttcaaaag attataataa tcaaatacta aatcggggat
     4621 agatgattac cttcaatctt ttagcttaaa aaggcttgaa actaaagtgt tcacattgaa
     4681 taagtctcta aacagcatga atcattaata aattcccgta cccgtttttg ttaaccattt
     4741 cgattaaaaa agtctcttca ctttttcttc tacatagttc ttattagggt ttaaatcaaa
     4801 aatgatttca ggttttagat agaataattc attttcattt ttaaatcgtt tttcaccgcc
     4861 accggtaatg tttaacatga tcacagcctc tttgtccaat tttttgtcct ttacttcggc
     4921 tattaaagta gcagtggcaa ttgccgctgc agggtgaata tcaattcctt cgtgcttttc
     4981 aaaaagtgcg gctgcctccc ttgcactttg attatcggct ttaagcacat cgccatcggt
     5041 atccagcagt gcgtcatata agccgcctat tatggaatat ggaggctttc tgttagaaag
     5101 cactttagca tctattatct ctactttaag ccgcgcttcg ttttcgtcaa agtcgagtat
     5161 ctttctggat ttactcttcc atgcatcata aattggcgta aatggttcat tttgggaaac
     5221 catcaacttc atcttattat ctccaaaacg gccgtcggca atcagacgta tattagcctc
     5281 ccatgctgca atggctccgg taccacttcc aacagcttga aaataataat ccggaatagc
     5341 gccaatagta gtaactgccg atagcactgt agtacccata ccatcgcgac gtgctacatt
     5401 cttagctcct ccttcagcaa caaaaccatc taagcgggca acaatagacg atagatgaat
     5461 agcatcataa taatcacccg aactacgtga tgagatcaac ttaacatttt cctttatcgg
     5521 atggttgaac cagagggagt ctatgtagtc ttcaggcacg caaagaagca aaggaatatt
     5581 attatcggaa cacacttttg caaaagctcg tgctgtattg ccggcagatg ccaccacaag
     5641 tatttttttg ttatccggct caagtcttgc acataccgaa taggcttcag tctccttaaa
     5701 agaacatgtc ggcattagat gtcctttttc cggccaatat ccgctaaaag ttatgtagag
     5761 attatgaagc cctaaatatg cagccaatcc tttactttta taggtgaatg gagctactga
     5821 accaaccaag aaacgattca caggtagcca atcagcaaat ttgtatagcc cgggattatg
     5881 gctatcagca tcgagttgtt gtttttgata aatcgccctg atcaatgtgg ggtcgttttc
     5941 tcctggcgct tcaagcaacc agccagtgtc ctcaaaagtt ctaccggtag caattgattg
     6001 gagcttataa agagtttgag tagaagtatg tttcattaat agaacattat atttttggca
     6061 aaaatataaa aagagctatt aaaccggatt aagttggtaa caattattga tcaggggtta
     6121 atcagatttt gaaataccac taactgttgt tcccttttgt agaaagtaaa aatttatcgt
     6181 ataagaagag cagcaaagat gttccgatac cgattccggc aaagaccatc cagatatttc
     6241 cagggctata ggttgtccaa aggtattctg ttagttgatt ttgagtcatg ttcatcaatt
     6301 cggctgaatg cgtaaggaga tcattttgag tgaaagtatc ggaaattgca ggaacatcaa
     6361 gcccgcgtag agctatctcc ttttgcagca aagtgatctt atccgacata tttccatata
     6421 cattaccgga gagtattcct gcaaaaaaat taccgccagc cataggcaaa aatgatgccc
     6481 ccatatacaa agccacttta ccctctggcg caattcttcc aatatattca gtgatctttg
     6541 gcgagctggc catttcaccc aagccgaaaa tcaaaataga aacaaacaga aaaaaaggat
     6601 tattggtata aaaagtaagc cccagcccaa tggaattcac caatattcct gatatcatag
     6661 cattcagcgc tttgtatctc ataacaaatg ctgacacaaa aatctgaaac ataattatat
     6721 agagcgcatc aagattgatt agcatctcag gagcaattgt gccactatta gtgccaacag
     6781 cacgtgcgaa agacggcgaa atacgaaaaa tagcctgata caacaacgat gtatccatcc
     6841 attgatcaat aaaaaccggt agagtataaa aaagctgatt atacattgcc cagaagccag
     6901 tgatgattag caagaagagt agcaacctga gatcgctcaa agcgcttcca atgtttttaa
     6961 aaattgtaat tattgattta aacaatggaa tagttgattt ttcgattaag ggctctttaa
     7021 aaaagaacaa tacaataaat aaattgacga gagaaactac ggatgatgta atgaaaacat
     7081 attgccaatc aatgcctctt aacttggatg ccaccaccgg cccgataaat gcgccaatat
     7141 tcaccatcat gtaaaaaatc ccaaatccta tagatgaagt ttcgctatta gtagttttag
     7201 caatggttgc tgatattacc ggcttaaaaa gggcagcgcc aacagccaca taaataaaca
     7261 caatatagag tgaggtgtag ttttttataa aactcatcat aaaatagcct gacgccaaaa
     7321 taatatacgc tatgatgagt attttacgat atccgtaacg atcagccaat gcgcctgtaa
     7381 taacaggcag aaaatacaaa atggctacaa ccggacccat taagtatcct ttttgctctt
     7441 gcgaaaaacc caatgcgccg ctatctttcg atcctgtaag atagagggca aataacataa
     7501 acataccata ccatgcccat cgttcgagca attccatcgt attggcagtc cagaaattct
     7561 ttggaaatgc ttttaaaacc cgacttatcc tgttcatgaa taaaaattat ttgaacatct
     7621 ttaataaaaa ccttttgcga tacttaggcc gcaaaatcaa taacactgtc agaataaaaa
     7681 taaatatcac taaacctgca attagcgtca aaacaatagc ccaaaaaaga ctcaatgaaa
     7741 ataatagttg ctcaccctgt ttgattaaaa tcctattttc ctttttaatc agatcatctt
     7801 tagcctttgt tgttatgtca aatttgttaa gctcctccgg gctaagctgt aagtattgtt
     7861 taagctttaa atatgaatcg agataccatt cggcgctatc agtcagaccg atggcgctat
     7921 atgaaaaaac catcagttta taattctctg ataattcgtg tagcatctgt gcagaatcag
     7981 taatttgaat gcttgtttta atatactcaa tagctgagtg ataattattc tctttaataa
     8041 gatttacacc cataaaggat aatgtagttg caacttctcg catattacca agtgtctgac
     8101 tgatcttcag gctactgata tatagttctt ttgcccggtc gggttgtcct aatttttcat
     8161 aagccatacc catcaggttt agtacgtaag ccatcccttt ctgatcatct aagctttccc
     8221 tgataagata agcagtagta agcatttcta atgcactgtt ggcctcatct tgttgcaggt
     8281 aaacattagc aatatttgta agaataacac cctgacgact tctattattc attttttttg
     8341 caagcgccag cgctgtccta tactcaacca aagcattttc gagcaaatct tgtgcttcat
     8401 ataaagcgcc aataccaata aatagatcaa ctttgatctg gtcattctcc attttttcat
     8461 tcaaaattat ggcctcccga taacactcca ttgagatcaa atattcgccg gtataataat
     8521 agcaattacc aagattatat aggcttctga ttatttcggt agtatcggcg tttatgcgat
     8581 aaaactgaag gatttcgcgc aaatgattga tagcttttaa aaagtcagct tgagccatat
     8641 agattagtgc aacattattt ttgaccttca ttaactcaga ctgatcgttg atagtttcaa
     8701 atagcttaag agccttatta tattttacta atgcgctatc gtatcttcca atgatatagt
     8761 ctataattcc aatatttttg agcgagatcg ccatgcctgt tttattatcc gaggttaccg
     8821 atatttcata ggctttaatg cagaaattta aagcgctttc gatatccgaa tttctgtatt
     8881 gacgagataa ctcattaagc tccataattt gctccaaagg ggttttgttc ttaattacct
     8941 cattgatgtc aggtgcatca gattgaccaa aaacgaagcc tatccgaaaa aataaaatga
     9001 tccagaggaa aaagatactt ttacttttca agtaataatt tatgtttatt ggtcttttta
     9061 tcatcgcctt gtgtatgaat cttcgacagt aaaataatgt tcaatttttt gaaaaagctg
     9121 ggggtagata aggcttatat tttgtaattc gtccacagaa ttaaattttc caaccttatc
     9181 tttgtattca aagatagctt tgacaatata attttcaagg taagggtgtg ctaacaactc
     9241 tttgtaaccg gctttattga gatgtaactg ctttatcttt tgtaaatcaa cttcaatata
     9301 aggcgagatt tgtgctaatc tggtagtatc cattccaaaa acttcaagta actgcgattt
     9361 gtcgaaatat ccgccaagtt tttcccgata gcttacaatc ctccgtgcaa aaacagtccc
     9421 aatacctttc aattgtttca gttcaatagt atcagccgca tttaaatcaa cgataatagg
     9481 agttttctct tcactaattt gaatacttgt tgaggtatca accgagggca acaaaatata
     9541 tttgtccaaa acagaaaaat catcttccga aatactatat aattttccaa aatcgctctt
     9601 tactttaaat ttacctcctt tacttctgta attgatgata ttacgtattt ggccatcctt
     9661 aagcccaagc ctctccattt ctgtgctatc aatcaggttg ggatcaaaag ggaagggatt
     9721 caagattttt gccggaatag ttttttcact tttctctaaa acatctttct ctttaaaaat
     9781 ctgaatataa ggtcgcaatg tattaaactc ttcatttgag atagaaaata ttttaccaaa
     9841 atcatcagcg gttctaaatt ttccaccggc atccctgtat tttatgatat tttccgctgc
     9901 ccgctcactt aaaccaagtt tgataaaaat ttctttcgag gcaaaattag ggtagaatgg
     9961 aaaagggtta agtaaaggtt ttgcagtagg ttcgggcgat aattgacgaa attgtaaagt
    10021 tatggaatca aatttggcat cccaaatgaa atcaggccga attatgaaag gtagaataaa
    10081 atagagaata attgttatga caatggccac aagcaggatc aacatcccat ttcgttgtcc
    10141 tcgactgata atgaagtatt cttttaatct tctattttta attaatttcg acaattagct
    10201 gtttttaaaa actgattaat tccaagctgc taaggaatac taaggcaata gccactgggg
    10261 caacaattcg gaggatgaac ataaaaatcc caaaaaatct acctttaagt tcaccattat
    10321 ttgtaatctc cgttctcaca ttctcttttc ccataaacca tcccacaaaa attactatca
    10381 tcaagcctcc taatggaagt aaaatattag cggaaataaa gtcaaggtca tcaaacaggg
    10441 tgtctttaaa cactttaaga taattcattg acccttgtga taaagtacaa agaacgccaa
    10501 gaactgaagt tgagccggca gcaatcacag tagctttttt tcgggacatt ttaagctctt
    10561 ccgaaaaata agcaaccacc acttcaagta aagatattgt agatgttaat gcggcaacag
    10621 caagaagcag gaaaaaactc aatgcccaga agtaaccgcc agtcatctgt tgaaaaatat
    10681 taggcaaggt aataaaagcc aggccgggcc ctgcatttgg cttaattcca aatgcaaaag
    10741 cagcaggaaa tattgccatg ccggctataa tagcaacaaa agtgtcagct ccggtaacag
    10801 ctaaagcggt acttgtaaga ttatcctttt tattgatata tgacccatag gtaattaatg
    10861 ttcccatgcc aatactcaaa gaaaaaaaca cctgaccaag ggcttttaga acagcctccc
    10921 acgtgatttt tgaaaaatca ggtttaaaaa gaaaaaccaa accctctttt gaaccgggaa
    10981 gtgtaattga acgtattgca attactacaa ttataagtaa caacaacggc ataaggatct
    11041 ttgaatattt ttctataccg tccttaattc ctcgccacac tattaatgcc gttaaaaaca
    11101 taaagataag ttgccaaata actggaaata gcccgttagc ctgaaagcta tcaaacgaac
    11161 tttttagttc ttcgaccgat tgccctgaaa aactgttaat cagcgaacga tatatatact
    11221 caagcgtcca tccggctatc gtggtataga aagcaagtat cataaacgcc gctgcaattc
    11281 caagtatccc aatgagatac caaggctggt taggctttaa taatctaaaa gcgccgtaag
    11341 gatttcgctg tgcgcgccgg ccgataatga attctgaaag cattaccggt actccaatga
    11401 gcaacattat cactaaatag ataacaagaa aggctccacc gccattttca cctgttacac
    11461 aaggaaacct ccaaatatta cccaatccga tggccgaacc tgcagcagca gcaatgacgc
    11521 cgatttttga agaaaaacta tcacgttgag agagtacaga cttttccata aaaaaaagat
    11581 atttcaattc ttaataattg atgcaatgat acaaaaaatg actatttgtg aacataattt
    11641 tttttagatc ctttttttaa ctaaaaaact gctttttaat aaacctaaaa tattttcaaa
    11701 t
//
LOCUS       DAOOAI010000074        11420 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_7241_length_11420_cov_5.521161, whole genome shotgun sequence.
ACCESSION   DAOOAI010000074 DAOOAI010000000
VERSION     DAOOAI010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11420)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11420)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11420
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7241_length_11420_cov_5.521161"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..576
                     /locus_tag="PK345_06330"
     CDS             <1..576
                     /locus_tag="PK345_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HPX34665.1"
                     /translation="MHPVIDLLAGIPSVIFGMWGILVIVPVISNYIGPLFGKNISGYS
                     ILAGAIVLSIMIIPFILNILIELFRHIPTELKETSLSLGATQWQTVKFVLVKKIFPGI
                     VSAFGLGMSRAFGETIAVLMVVGNVVHVPKGIFQPGYPLPALIANNYGEMLSIPLYDS
                     ALMLAALILFVIVLFFNFLSRLIIVKSNIHN"
     gene            581..1435
                     /gene="pstA"
                     /locus_tag="PK345_06335"
     CDS             581..1435
                     /gene="pstA"
                     /locus_tag="PK345_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013443576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HPX34666.1"
                     /translation="MDLRKIEEIFFRILMFCSTVIIAVSLIIIIFSTFYKGLPHLTWE
                     MISKTPKGGFYFGKEGGILNAIVGSLYLSFGATILAILFGLPLALFLNVHIAHRRKLV
                     NVVRFVMDLLWGVPSIVYGAFGFTIMVFFGIRASLLAGIITVTLFIIPIMVRGIDEVL
                     QSVPRGLHESSLSLGSTRAETAYKVFLKQSVPGVVTAILLSFGRAIGDAASILFTTGY
                     TDHIPTSLSQPTATLPLAIFFQLSSPIPQVRDRAYAAAAVLFIIILIISILSRFFSKR
                     YQKNKINF"
     gene            1438..2196
                     /locus_tag="PK345_06340"
     CDS             1438..2196
                     /locus_tag="PK345_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HPX34667.1"
                     /translation="MNKPKINIQDLNLYYGKQHILKNINTIIPERQITVIMGPSGCGK
                     TTLMKSLNRLTDLTPDCSVSGKIMIDGQNILNTTEDITKIRKKMGLLAQRPYPLPMSI
                     YNNVAYGLRISGRKKRRFLDKRVEHYLRQVNLWNEVKDRLKEPAGTLSIGQQQRLCLA
                     RGLAVDPEIILADEPTSALDPISSQTIEEKFKELKNQFTIVMVTHILRQAKRIADYVI
                     FMYLGEIIEQGPAKDFFENPQEEITKDYLKGLFY"
     gene            complement(2318..3577)
                     /locus_tag="PK345_06345"
     CDS             complement(2318..3577)
                     /locus_tag="PK345_06345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014774463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase N-terminal domain-containing
                     protein"
                     /protein_id="HPX34668.1"
                     /translation="MFFFYNLFIQLYNLTIWLSKPFNNKARMWIAGRKDVFKKIIKRV
                     EHDKPIVWFHCASLGEFEQGLPVIESFKETFPDKQIILTFFSPSGFEIRKNYPGADYI
                     FYLPADTRRNARRFIDLVKPEIAVFVKYEFWFNYMRELKRSYVPYIFISTIFRPKQHF
                     FRKWGRWQIEQLRQADHFFVQNQSSADLLQKHGIEQVTLSGDTRFDRVHNIAKQKRVF
                     PPIEIFKGESKIFLAGSTWQPDEELITELIKLNFDGLKIIIAPHEVHEARIQTLMRKL
                     PDSAIRYSMADTSNVTEAPVMVIDTIGILSHIYKYATLAYIGGGFGSGIHNTLEAATF
                     GVPVIFGPNFNKFHEAVDLIERGGAYNISSKSEFIEVINTLLNNQKKYEASSFAARNY
                     VEQKQGATGIIVNYLKNISNNLTNFEK"
     gene            complement(3603..4364)
                     /locus_tag="PK345_06350"
     CDS             complement(3603..4364)
                     /locus_tag="PK345_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34669.1"
                     /translation="MKYVIILSICFFAFTGLAHAQAEGAAKNEIENVIMVFPAVSFQL
                     PAGDMSERFGSNSTIGMGYQHKTGGNWLFMADYNYIFGNKINQEGLIQNLLTVDGFVI
                     GDNGQIAEVSFFERGFYTTLRAGKLIPLTASDPNSGLMLLAGTGFMQHKIFIKVKDNM
                     VAALRKDYNKGYDRMTNGFSAVQFIGYMRMDKKRLHNFFAGIEIVEGWTKGRRSINFD
                     TMTIDDNQRLDILYGIKLGWIIPFRKRMAKDYYYY"
     gene            complement(4425..5096)
                     /locus_tag="PK345_06355"
     CDS             complement(4425..5096)
                     /locus_tag="PK345_06355"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HPX34670.1"
                     /translation="MHGVTPNRLLSYNFVSIEGNIGAGKTSLATRIAHDFNAKLILEQ
                     FEENSFLPKFYKEPDKYAFPLELSFLAERFEQLKQELTHRDLFTNFTVSDYFIHKSFI
                     FARKNLPDDTFGLYKKLFEIISETLPRPDLLIYLYLDVDNLLKNIKKRGRDYETYIKA
                     EYLEKIQTSYLEFIRQQSNMRIVIVDTNNLDFVSNDEDYLLMVNVLNRHYEVGVHLIS
                     PKSEQ"
     gene            complement(5259..5759)
                     /gene="folK"
                     /locus_tag="PK345_06360"
     CDS             complement(5259..5759)
                     /gene="folK"
                     /locus_tag="PK345_06360"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HPX34671.1"
                     /translation="MQKSYLLLGSNLGDKNHNLLKARTLIQSKEITLVRESAIYKTSP
                     WGFDADEDFLNQVVEIATELDPMKLLNRLLEIEKQVGRKRKKIAGYQSRILDVDILFF
                     GSQVINTPKIIIPHPRLHLRRFTLVPMNELAPDLMHPVLNKTIAQLLAECDDNMKVVR
                     IFTSTK"
     gene            5862..7637
                     /gene="sppA"
                     /locus_tag="PK345_06365"
     CDS             5862..7637
                     /gene="sppA"
                     /locus_tag="PK345_06365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HPX34672.1"
                     /translation="MKQFFKFMFASMLGFLLMSIVIFFLFLGMVSSLAKFMEKETAKV
                     AENTVLHVKFDAEIRDRTSKDPFSDFDYNTMQPKSSLGLNDILKSLEKASNDPKIKGI
                     YLDLSTVKSGGEFLEEIRTALLKFKESEKFIIAYGESYSQNAYYLGSVADKVYLNPEG
                     SIDFKGISAEVTFFKEMIDKIDAEMQIIRHGKYKSAVEPFMLDKMSSENREQMMALVT
                     GIWNTYLNSISESRNITTSELNRIADNLLIRTAKDAVQFNFVDDLKYYDEIIAELKSL
                     CGTKEGDELNSIKLSKYFTAPSDSKEKKDFQNRIAIVYAVGEIITGQGNDDVIGSDRI
                     AKAIRDARKDEKVKAIVMRVNSPGGSALASDIILREVKLAAAEKPFVVSMGGVAASGG
                     YYISAGATKIFAESSTITGSIGVLGVLPNLEKTMKNKLGITFDYAMTNENSNHMSVFR
                     PMTQTQRDNIQSAIEDIYSTFVNHVATGRNMTYEQVDEIGQGRVWIGTDALKIGLVDE
                     IGGLNDAVEAAVKLANLDTYSIKELPKQKDFFKELFDEMFGNASLEAKLQRELGNDYK
                     LYQQLRYWRNAKGIQARMPFDIHLN"
     gene            complement(7642..11280)
                     /locus_tag="PK345_06370"
     CDS             complement(7642..11280)
                     /locus_tag="PK345_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPX34673.1"
                     /translation="MPKFKITLLIFTAFIFTFSWSALMAQKQLDYELHFKNMSFTPDS
                     QIGNLTLQSIPADQLFEGKYFTIIQFYSIPSELDRSNLSDAGVLLLDYIPHYSYFTCF
                     EPGFNPNVLTNANVRAIVSITEAVKLSPQLFVDEIPDHAFKEDGRVELLVGHYPNLNR
                     EKVAQALVSAGFEVLLNDSFGEFIPVLSSVENIKYLARQPYVVYVEPIYPTPEPDNYS
                     GRTLHRSNGLASDFSTGRHYDGTGVNVMLQDDGIIGPHIDYQGRILDQYLGNNNGNHG
                     DHCAGIIMGGGNLDPRARGNAFGANIYVYSANNYSGFLNIPSHYSSNQIRVTSTSYSN
                     GCNAGYTSLTRTMDQHMRMYRKLLHVFSAGNAGGEDCGYGAGVGWGNITGGHKAAKNV
                     FTVANLNETDGLNSSSSRGPAHDGRIKPEISAKGTDVYSTIDPNSYDFKTGTSMSCPG
                     VAGVVTQLFHAFRELNDGNDPTGGLIKAIVMNTAEDLGNPGPDFKFGFGRINGRRAVE
                     VLEQGRYDSAVINTNQIIEHQFEVPQNTAEARIMVYWVDFEATVNTNWALVNNLDIKV
                     TDPSGNEWLPWVLSHYPKHDSLNKPAVRGIDDRNNQEQVTLSFPEPGQYTLTVTGTTI
                     PQGPQTYYIIYDFVPDEVVLTYPFGGELWRPGADELLRWDASGNEGLFTLEYSLDNGN
                     SWYVIKNDVAGTARHYNWIVPSTLTGQALVKISREGSTSQCNVPITIAPIPTELKVDW
                     ACANDIHLSWNEVYGASGYVIYKLGEKYMEPVGTTNINSFILEDSPSNLTYWFSVSAI
                     TPDGAVGQRANAVQKLPGTLNCHTTDAKMLDAPSVDWGLFQTCMEISNLDVTIRMKNF
                     GLEPIINPTFHFQLNDGEIVNEVYQGTIMPDSLLTFTFSQGISINNIGTYSLKSWVEY
                     APDTNPQNDEIITNFEVIEGTTILPGTIQTMESFQQCLPVPICELYSCNLDDGWINLT
                     NQVQDDIDWRTWKGSTYSFNTGPSFDHTIGTISGQYLYLEASTICFNKEAILSLPCID
                     LTNRLMPVFSVWYHAFGADMGRLHVDLFDGSQIIRDIVPPVIGDQGDEWRQLTADLSP
                     WTGKTIGIRIRGITGSGDKSDLAIDDIAVLDVTSVNSLKTANDELKVYPNPGKGVFNI
                     TLDTQATGNFQLSVTDITGKVRHKENIAISNGQFYKALDLGKLPNGVYFLELKSDKAV
                     YQTKLLKN"
BASE COUNT         3484 a         2173 c         2171 g         3592 t
ORIGIN      
        1 atgcatccgg taattgatct tttggcaggc ataccgtcag tgatctttgg gatgtggggt
       61 atcttagtaa ttgtaccagt aatttcgaat tatatcgggc cattatttgg aaaaaacatt
      121 tcgggttatt caatattagc cggtgcaata gtcctttcga taatgattat ccctttcatt
      181 ttgaatattt taattgaact attccgacat atcccgacag agcttaaaga gacctcttta
      241 tctcttggag ctacacagtg gcaaactgtg aagtttgtac ttgtcaaaaa aatatttccc
      301 ggaatagtct ccgccttcgg attggggatg tcaagggcat ttggagaaac aattgcggtt
      361 ttgatggttg taggaaatgt agtacatgta cctaaaggaa tatttcaacc tggttatcca
      421 ttgcctgcat taattgccaa caattatggt gaaatgcttt cgatacctct ttacgattcg
      481 gcacttatgc tggctgcact tatacttttc gttattgtcc tttttttcaa ctttttatcc
      541 cgattaatca tcgtcaaatc aaacatacac aattaatttc atggacttac gcaaaataga
      601 ggagatattt ttcaggattt tgatgttttg ttcaacggtc atcatcgccg tatcacttat
      661 cattataata ttcagcacgt tttataaagg gcttccacat ttaacatggg agatgattag
      721 caagacgcct aagggcggat tttattttgg gaaagagggc ggtattctga atgctattgt
      781 tggttcgctt tatttatcat ttggggcaac aatactcgca attttatttg gattgccgtt
      841 agcgcttttt ctaaatgtac atattgctca ccgacgcaaa ttagtgaatg tagtaagatt
      901 tgtgatggat ttactatggg gtgttccctc catcgtctat ggcgcatttg gctttacgat
      961 aatggttttt tttgggatca gagcttcgtt gcttgctgga attattaccg taaccctctt
     1021 tatcatccca atcatggtca gggggattga cgaagtacta cagtcggttc cgcgcggctt
     1081 acacgaatca tctctatcat tggggtcaac gagagccgaa acagcttaca aagtattttt
     1141 aaaacagtcg gttccgggtg ttgttactgc tattttgcta tccttcggac gagctatcgg
     1201 cgatgcagct tctatcctgt ttaccaccgg ctacaccgat catatcccaa catctctcag
     1261 ccagccaacc gccacattac ctctggcaat cttctttcag ttaagctcac cgatacctca
     1321 agtaagagac agagcttatg ctgcagccgc tgtcttattt atcatcattt taataatcag
     1381 catattgtca aggtttttta gtaaaagata tcagaaaaat aaaatcaact tttaaccatg
     1441 aataaaccta agatcaatat acaggatttg aatctttatt acggcaaaca acatattctt
     1501 aaaaatatca ataccattat ccccgaacgt caaataacgg ttatcatggg gccttcgggt
     1561 tgtggtaaaa cgaccttaat gaaaagcctt aacaggctaa ctgatctcac tcctgattgt
     1621 tctgttagtg gtaaaataat gattgatggg caaaatattc ttaatactac cgaagatatt
     1681 actaaaatcc gaaaaaagat gggccttctg gcgcaaaggc catatccatt accgatgagc
     1741 atttacaata atgtcgctta tggtttacgt attagcggaa gaaaaaaaag gcgttttctc
     1801 gacaaaagag tagaacacta tcttcgtcaa gtaaatctat ggaacgaggt gaaagaccgt
     1861 ctgaaagaac ctgccggaac actttctatt ggccagcaac aacgtctgtg ccttgcaaga
     1921 ggtcttgccg ttgatcccga aataattctt gccgatgagc ctacttcagc attagacccc
     1981 atttcgagcc agactattga ggagaaattt aaagagttaa aaaatcaatt taccattgta
     2041 atggttaccc acattctccg acaagcaaaa cgaatagcag attatgtgat ttttatgtat
     2101 ttaggcgaaa taatcgaaca aggcccagct aaagattttt ttgaaaaccc tcaagaagag
     2161 atcacaaaag actatctgaa aggattgttt tattaataga ggaaagtctt tacctaaagg
     2221 aggtttaata gattcaattt tgagtcagtt ccaaaaatga aatttttcag attttcgtgt
     2281 atggctgctg aacgaaggtg aagtattcgt gttaaaatta cttttcgaag ttggttaaat
     2341 tattagatat attcttcaga taattaacaa ttattcctgt tgcaccctgc ttttgttcaa
     2401 cataatttcg tgcagcaaat gacgatgctt catatttttt ttggttgttt aacaatgtgt
     2461 ttatcacttc aataaattcg cttttcgaac ttatgttgta tgcgccgcca cgttcgatta
     2521 gatcaaccgc ttcatgaaat ttgttaaagt tgggaccaaa gattacaggg actccaaatg
     2581 ttgccgcttc aagggtatta tgtatgccgc tcccaaatcc accgccaata taagctaacg
     2641 ttgcgtattt atagatatga gataaaattc cgatagtgtc aattaccatt actggtgctt
     2701 ccgttacatt gctggtatca gccattgaat atctgattgc tgaatcgggt aattttcgca
     2761 tcaaagtttg aatgcgagct tcatgcactt cgtgaggcgc aataattatt tttaagccat
     2821 cgaaattcaa tttaattaat tcggttatca attcctcatc aggttgccat gtgctaccgg
     2881 ctaaaaagat tttgctttcc cctttaaaaa tttcaattgg tgggaaaacc cttttttgtt
     2941 tggctatatt atgtacacga tcgaaacgtg tatcgccact taaggtaacc tgctctattc
     3001 catgtttttg taaaaggtct gctgacgatt ggttttgtac aaagaagtga tctgcctgcc
     3061 gtagttgttc aatctgccat cttccccatt tccgaaaaaa atgttgttta ggtcgaaaaa
     3121 ttgttgaaat aaaaatataa ggcacatagc ttctttttag ctcacgcatg tagttaaacc
     3181 aaaactcata ctttacaaaa acggcaattt cgggctttac taaatcaata aaacgcctgg
     3241 catttctacg ggtatcggca ggcaggtaaa atatataatc ggctccgggg taattttttc
     3301 taatttcgaa acctgatgga gaaaagaaag taagaattat ctgtttgtca ggaaatgtct
     3361 ctttaaaaga ttcaattacc gggagcccct gttcaaactc ccctaacgaa gcacagtgaa
     3421 accatactat tggcttgtca tgttcaaccc tcttgatgat ttttttaaaa acgtccttcc
     3481 tgccagcaat ccacattcgg gctttgttgt taaatggttt gctaagccat attgtaaggt
     3541 tgtagagttg gatgaagagg ttatagaaga aaaacattac caataaaaat ttgaattaca
     3601 ggctaatagt aataataatc ttttgccatt cgttttctga aagggataat ccaacccaat
     3661 tttattccat aaaggatgtc aagtcgttga ttatcgtcaa tagtcatggt atcgaaattt
     3721 atagagcgcc gccccttcgt ccatccctca acaatttcga taccggcaaa aaagttatgt
     3781 agtcgctttt tgtccatcct catatatcca ataaactgta cagcgctaaa accattagtc
     3841 attcggtcgt atcctttatt ataatcttta cgcaaagctg caaccatgtt gtctttaact
     3901 ttaataaaaa ttttgtgttg cataaaacct gtacccgcta acagcatcaa ccccgaatta
     3961 ggatcagaag cggttaaagg gatcaatttt ccggctctga gtgtggtata gaatcctcgt
     4021 tcgaaaaaac tcacctcagc tatctgacca ttatctccaa taacaaaacc atcaacggtt
     4081 aacagatttt gaatcagacc ttcctgattg atcttattac caaaaatgta attataatca
     4141 gccataaaaa gccaattacc accggtttta tgttgatatc ccataccgat tgtggagttg
     4201 ctaccgaagc gctcggacat atcacctgca ggaagctgaa agctaactgc cgggaatacc
     4261 atgatgacat tctctatctc gttctttgca gcgccctcag cctgtgcatg agctaaacct
     4321 gtgaaagcaa aaaaacatat tgaaagtata atgacgtact tcatataaca atattgatca
     4381 acaaagataa agctttggtt ttggcgtgag tatttaacaa acatttattg ctctgatttt
     4441 ggagaaatta aatgaacccc aacctcgtaa tgacgattta aaacattgac catcagtaaa
     4501 tagtcctcat cattcgaaac aaaatcaaga ttatttgtat ctactattac aattcgcata
     4561 ttcgactgct gacggataaa ttcaaggtaa ctggtttgta tcttttctaa atattcggct
     4621 ttaatatatg tttcgtagtc gcgccctcgt ttttttatgt tttttaaaag attatctaca
     4681 tcaagatata gatagatgag caagtcaggt ctgggtaagg tttcgctgat gatctcaaaa
     4741 agctttttgt aaagtccgaa agtgtcgtca ggaaggtttt ttctggcaaa gatgaaagat
     4801 ttatgaataa aatagtcgga aacagtaaag ttggtaaata gatcgcgatg ggtaagttct
     4861 tgttttagtt gttcgaacct ttcggcaaga aaagagagct caagagggaa ggcgtatttg
     4921 tcgggttctt tataaaattt aggtagaaat gagttctctt caaactgttc taatatcagc
     4981 tttgcattaa aatcatgggc tatccgtgta gcaagagatg ttttaccagc tccaatattt
     5041 ccttctattg atacgaagtt gtaagacaaa agtctatttg gtgtaacgcc atgcataagt
     5101 attaaataaa aaatgttcaa cgattctttg ctctatattt ttcatgattc attattacaa
     5161 tcaaatgaat tgtggataaa actttatttt aatcgtagta tgtgaagcat tgtcaatagt
     5221 tctttgttgt ttttattcct gtaatttatt tgtaaaaatt attttgttga cgtaaagatt
     5281 cgcacaactt tcatattgtc atcacattcg gctaaaagct gtgctattgt cttatttaaa
     5341 accggatgca taaggtcggg tgcaagctca ttcattggca caagagtaaa tcgtcgtaaa
     5401 tgtaatcttg gatgaggaat aataattttt ggggtgttaa ttacttgact gccaaaaaaa
     5461 agtatgtcaa catccaatat tctggattgg tatcctgcta tttttttcct cttacgtccg
     5521 acctgttttt ctatctctaa aagtctgttt aataatttca tcggatctaa ttccgtagct
     5581 atttcgacca cttgattaag gaaatcctca tcagcatcaa aaccccatgg cgatgtttta
     5641 tatatggctg attccctaac taaagtaatc tcttttgatt gaattagcgt acgggctttc
     5701 aacaagttat gatttttatc accaaggttt gagccaagta agagataact tttttgcatt
     5761 tttgagaaaa aatgattgaa atcgtgtcaa aaataagaca acttttaaaa attaatgcca
     5821 cctttgccat tattaattat tcattttaaa atacgtaagt tatgaaacag tttttcaagt
     5881 tcatgtttgc ctccatgtta ggctttttgt taatgtcaat tgttatcttt ttcctgtttc
     5941 tgggcatggt cagctcattg gcaaaattta tggaaaaaga gactgctaaa gttgctgaga
     6001 ataccgtttt acatgtgaaa tttgatgctg agatacgcga cagaacatct aaagatccat
     6061 tttcggattt tgattataac actatgcagc ccaagtcttc attaggatta aacgacatcc
     6121 taaaaagtct ggaaaaggcc tcaaacgatc caaagatcaa aggcatctat ttagatttga
     6181 gtacagtaaa atcgggggga gagtttttgg aagaaataag aactgccctt ttaaagttta
     6241 aggaatcaga gaaatttatt attgcttatg gcgaatcata ttcacaaaac gcttattatc
     6301 ttggatccgt agccgataag gtttacctga atcccgaagg gagtattgac tttaaaggta
     6361 tttcggctga agtaaccttt tttaaggaaa tgattgataa gatagatgca gaaatgcaga
     6421 ttatcagaca tggtaagtat aaaagcgccg tagagccgtt tatgctcgac aaaatgagtt
     6481 ctgaaaaccg tgagcagatg atggcattag taacaggaat atggaataca tatctcaata
     6541 gcatctccga atccagaaat attaccacca gtgagcttaa caggattgcc gacaatttat
     6601 tgatcagaac agcaaaagac gctgtacaat tcaattttgt tgatgatttg aaatattatg
     6661 atgagatcat tgccgagttg aaatcactct gtggtacaaa agagggtgat gaactgaatt
     6721 cgattaaatt atcaaaatac tttaccgctc catctgatag taaagagaaa aaagatttcc
     6781 aaaatcgaat agctattgtt tatgctgttg gagagatcat aacaggtcag ggtaatgatg
     6841 atgtgatcgg ttctgataga attgccaagg ccattcgtga tgcccgcaag gatgaaaaag
     6901 taaaagctat tgttatgcgt gtcaactcac ctggtggtag cgctcttgct tccgacatca
     6961 ttttgcgtga agtgaagtta gctgctgccg aaaagccttt cgttgtatca atgggcggtg
     7021 tagctgcttc aggaggttat tatatctcgg ccggagcaac aaagattttc gccgagtcct
     7081 ccacaatcac cggctcaata ggggtgcttg gtgtgttgcc aaaccttgaa aaaactatga
     7141 aaaataagtt ggggataaca tttgactatg ccatgacaaa cgaaaactct aaccacatgt
     7201 cggtattcag gccaatgact caaacccaac gagataatat tcaaagcgcc attgaagata
     7261 tttacagcac gtttgtcaat catgttgcta caggccgcaa tatgacttac gagcaagttg
     7321 atgaaattgg acaaggtcgg gtatggattg gtaccgacgc tctgaagata ggcctcgttg
     7381 atgaaattgg cggtcttaac gatgctgtcg aggcggctgt aaaattagct aacctcgaca
     7441 cctattccat caaagagcta cctaaacaaa aagatttttt taaagagctg tttgacgaaa
     7501 tgtttggaaa tgcatcactt gaggctaaac tccaacgcga acttggaaat gactataagc
     7561 tctaccaaca attgcgttat tggcgtaatg ctaaaggaat tcaagccaga atgccatttg
     7621 atatccattt gaattaaatg gttaattttt caaaagtttc gtttgataaa cagctttatc
     7681 cgactttaat tctaaaaagt aaacgccgtt tggcaacttg ccaagatcca acgccttgta
     7741 aaactggcca ttgctgattg ctatattttc tttatggcga acttttccgg taatatcggt
     7801 aaccgataac tgaaagttcc ctgtagcctg tgtatccaaa gttatattga acacgccttt
     7861 tcccgggtta ggatatactt ttaattcatc atttgcagtc tttagactat tcactgaagt
     7921 tacatcaagc actgcaatgt cgtctatagc cagatcactt ttatcaccgc ttccggttat
     7981 acctcttatt ctaatgccta ttgtttttcc ggtccatggc gatagatcgg cggtgagttg
     8041 ccgccattca tcaccttggt cgccaattac agggggtaca atatcacgaa taatttgact
     8101 gccgtcaaat agatccacat gtaatctccc catgtcagcg ccaaaagcat gataccaaac
     8161 actaaatact ggcatgagtc ggttggtaag gtcaatgcaa ggtagcgaaa gtatagcctc
     8221 tttattaaaa caaatagttg atgcttctaa atagagatat tgccctgata tagttccgat
     8281 tgtatgatca aatgatggac ctgtgttgaa ggagtaggtt gagcctttcc aggttctcca
     8341 gtcaatatca tcctgaacct gatttgtaag attaatccaa ccatcgtcaa ggttgcagga
     8401 ataaagctca caaattggaa ctgggaggca ttgctgaaac gactccattg tttgaatagt
     8461 gccgggtaaa atagtagttc cttctattac ttcaaaattt gtgattattt cgtcattttg
     8521 tggattagta tccggcgcat attccaccca tgattttaga gaataagtgc caatgttgtt
     8581 aatgctgatg ccttgggaaa aggtaaaagt aagcaatgaa tcgggcatga ttgttccctg
     8641 gtaaacctca tttacaattt caccatcatt gagttgaaaa tgaaatgtag gatttattat
     8701 tggctcaaga ccaaaatttt tcattcggat tgtaacatca aggtttgata tttccataca
     8761 cgtctgaaac aaaccccaat caacagaggg ggcatcaagc atctttgcat cagtagtgtg
     8821 gcagtttaat gtgccgggta gtttttgaac agcattcgca cgttggccta cagccccatc
     8881 gggagtgatc gcactaacac taaaccagta agttaggttt gaaggggagt cttcaaggat
     8941 aaacgaatta atatttgtgg taccaactgg ttccatatat ttctcgccga gtttatagat
     9001 tacatatcct gaagcgccgt aaacctcgtt ccaactcaaa tggatatcat ttgcgcaagc
     9061 ccagtcaact ttaagctcgg ttggtattgg tgcgatggtg ataggtacat tacactgcga
     9121 agttgatcct tcacgcgaaa tctttaccaa tgcttgccct gtaagagttg aaggaactat
     9181 ccagttgtaa tgtcgtgcag ttccggcaac atcatttttt atcacatacc aggaattacc
     9241 gttatccaac gagtattcta aagtgaaaag gccttcattt ccggaagcat cccatcttag
     9301 taattcatcg gcgccaggcc tccaaagctc tccaccaaaa ggataagtta aaactacctc
     9361 atccggtaca aaatcataaa taatataata agtctgaggg ccttggggaa tagttgttcc
     9421 ggtaaccgtt agggtatatt ggcctggttc gggaaacgat aaagtaacct gttcctgatt
     9481 gtttctgtcg tcaattcctc taactgccgg tttatttaag ctgtcgtgtt tcggataatg
     9541 tgagagtacc cacgggagcc attcattgcc agaaggatca gtaactttta tatcgagatt
     9601 attaactaaa gcccagttgg tgtttacagt tgcttcaaaa tcaacccaat aaaccattat
     9661 acgggcttct gcggtattct gaggaacttc aaactgatgt tctattattt gatttgtatt
     9721 gattacagcc gaatcgtaac gcccttgttc caatacttcc actgctcttc taccatttat
     9781 tcttccaaat ccaaatttaa aatcagggcc aggatttcca agatcttctg ccgtgttcat
     9841 aacgatagct tttatcaggc ctccagttgg atcattccca tcgttaagct ctcgaaatgc
     9901 atgaaataac tgcgttacta cccctgctac accagggcat gacatagaag ttccggtttt
     9961 gaaatcgtaa gagttaggat caatcgttga atatacatcg gtacctttag ctgaaatttc
    10021 cggtttaatt cttccatcat gtgctggtcc acggctactt gagctattca aaccatccgt
    10081 ttcattcaga ttagctacgg taaaaacatt ttttgctgct ttatgtccgc cggtaatatt
    10141 accccatccg acgcctgcgc catatccgca atcttctcct ccagcatttc cggcagagaa
    10201 aacatgcaac agctttctat acattctcat gtgttgatcc attgttcggg ttaaagaggt
    10261 gtatcctgca ttacatccat tgctataact agtagaagta acacgtattt ggtttgaaga
    10321 ataatgagag gggatattaa ggaaacccga atagttgtta gcgctataaa cataaatatt
    10381 tgcgccaaaa gcgtttcctc gggctctcgg atccaaattg ccgccaccca taatgattcc
    10441 tgcacaatga tcaccatgat taccattatt attacctaaa tactgatcca gtattcgacc
    10501 ttggtaatca atgtgagggc ctataatacc atcgtcctgc aacataacat ttactccggt
    10561 accatcataa tgacgaccag tggaaaaatc acttgctaaa ccattcgaac gatgaagggt
    10621 tctgccacta taattatcag gttctggtgt gggataaatg ggttcaacat aaaccacata
    10681 gggctgacga gctaaatatt tgatattttc gactgatgaa aggacaggga taaattcccc
    10741 gaaactatcg tttaataata cttcaaaccc ggctgaaact aaagcttgtg caactttttc
    10801 tctattgaga ttcggataat gacctactaa aagctcaacc ctgccatcct ccttaaaagc
    10861 atgatcaggg atttcatcaa caaacaattg aggcgataat ttgacagctt cagtaatgct
    10921 tacaatagct ctaacatttg catttgttaa aacattcgga ttaaatcccg gctcaaaaca
    10981 ggtaaaataa ctataatgag ggatataatc aaggagcagc acgcctgcat cggaaagatt
    11041 acttcgatca agttctgagg gtattgaata aaactgaatg atcgtaaaat atttgccttc
    11101 aaaaagctga tcggctggga tactctgtaa agttaagtta ccgatctgag aatcaggagt
    11161 aaaactcatg ttcttgaaat gtaactcata atctaattgt ttttgagcca tcaatgctga
    11221 ccacgaaaag gtgaaaataa aggctgtgaa gatcaataag gtaattttaa atttaggcat
    11281 cttttcgaaa atttatggga ttaatactta taataaaatt attaaaacta aaacatattg
    11341 ttgaccaaga ttgttttaaa cttcagcttt tttgagagta ttactattct caaaaaaacc
    11401 tctttttaac attgtaattt
//
LOCUS       DAOOAI010000075        11287 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_7332_length_11287_cov_5.324163, whole genome shotgun sequence.
ACCESSION   DAOOAI010000075 DAOOAI010000000
VERSION     DAOOAI010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11287
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7332_length_11287_cov_5.324163"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            401..1294
                     /locus_tag="PK345_06375"
     CDS             401..1294
                     /locus_tag="PK345_06375"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34674.1"
                     /translation="MKKTLLLVVLLSAFSLVHAQEYVPFPTSNVYWNEIELYQGQCDP
                     PEYCKNTFYLMGDTVIDSYLYKKIYSNDGNSTSYVGGLREENKRVFLYYTWCDHSVLL
                     YDFNLNVGDTMFMSCLALECDINENNYMTVVSVDSVLLEDQSYRRRINFDSGPHMSWI
                     EGVGSVSGLLYPHYSCILCICFRELICFQEDVNTLYLNEENAACFNYCVSVSDLKNND
                     KMFQVYPNPAKYQTSVTIKSNEGINRVELYDFLGRMIACEQKINSMEYKLQLGNLRQG
                     VYFLRANLQNSQIVSNRLIIN"
     gene            1410..1967
                     /locus_tag="PK345_06380"
     CDS             1410..1967
                     /locus_tag="PK345_06380"
                     /inference="COORDINATES: protein motif:HMM:NF015863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY-associated TM helix domain-containing
                     protein"
                     /protein_id="HPX34675.1"
                     /translation="MTENNKRQRQANLIRAFRKIHRMTGVLLFVFFFINSITGLLLGW
                     KKHSGGLLLPESYKGTSTELKDWLPLDSLHKNACKILHDSVSTDLSLELERIDVRKDK
                     GMVKFVFNDHFWGIQLDGATGKLLQIERRNSDMIENIHDGSILDHYLGTTGEPIKVVY
                     TTIMGLGLLIFTITGFWLWYGPKRM"
     gene            complement(2158..4209)
                     /locus_tag="PK345_06385"
     CDS             complement(2158..4209)
                     /locus_tag="PK345_06385"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HPX34676.1"
                     /translation="MNIKTILFLTIFLIISSWANAQILIKKSEKIVNINGKEYYLHTV
                     EKGQTLFSISKTYNVDVRDIYEANPELENTLSSGQKIYIPTHRIHSIQFGSESNNLHK
                     HQVAKGETLYGLAKKYNISIEAIRAANTGLEDGLIEGMEIIIPDFESTNKKTEPQTKT
                     YENGGQGKPEKGYFEFQERNHETIYELAIRYRVSVDSILFLNPGLGEQLSKGEIIKIP
                     NNPTKKGFITHNIAQRITINRLARNYGLSQEEIQKANPYISRQLQPGQTIKIPLPPLK
                     NIESEITLPPEIYTIEENKKPYTPPVTSKDLCAQIPAAQTFDIALLIPLYFDELVEKT
                     ITTPSTSKQDKIPNSKSFQFIKFYEGFRLAVDSLEKVGLEARIHVFNVEEDVSQAQQC
                     LKNPALKKMDLIVGPFFNSSFRIVAEFALKHKIPIINPLSAKSDVINDNPFVIKVIPN
                     EKRIYNTIAEFISYKLSDSKIFIARQSKMRDEEHIINLRSALFENLENRWQQIVEFNI
                     QNDSLSVFNREALKERPNVIIVYSENKVVILDLLRKLNLIRNRYDITVIGLPDWLEME
                     GLDFNHLGNLNTHFPAAEFADYHSEEVKKFVRRFREYYFTDPDAYAFKGFDTGIFFLS
                     ALMNFGSNFMTCLPYHDIPLLYSNYKFITDNENGFENQSWKILRLSNYRLYEVPVPVR
                     R"
     gene            complement(4346..5875)
                     /gene="guaA"
                     /locus_tag="PK345_06390"
     CDS             complement(4346..5875)
                     /gene="guaA"
                     /locus_tag="PK345_06390"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009275897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HPX34677.1"
                     /translation="MLEIILIVDFGSNFTQMIARKTRELKVYCEIYPYNKIPTNKNIK
                     GIILSDSSYSIDDPNSPKPDFDQLFKADIPILAIGYSAQRLAQHLGGQIKKSATSKNV
                     IANFGDIDPSNTLVQSIKLGSQVLSAGGSVILSIPDNNKIIMRTETSEIVGFQVNSRN
                     IFGIQFHPVADQTADGVQLLENFVVSICQCRQDWTPARFIHDSTKSLKDQLQDDKVVL
                     GLSGGVDSSVAAMLLHKAIGKNLHCIFVDNGLLRKNEFASVLESYHDIGLNIKGVQAA
                     DRFYKALAGITDPEKKRKAIGHIFIEIFDDEAHKIKEVRWLGQGTIYPDIIESAPIPG
                     STATIKSHHNVGGLPNYMKLKIVEPLKNLFKDEIRRVGFELGLKPEILNRHPFPGPGL
                     GVRVLGEITAERIKILQEADYIFIKKLKECGLYDKVWQALAILLPVQSVGVSNEKRTY
                     ENAIALRAVHSTDGMTAEWAHLPYDFLEKVSEEIIRNVKGINRVVYDISSKPPATIEW
                     E"
     gene            complement(5888..7831)
                     /gene="yidC"
                     /locus_tag="PK345_06395"
     CDS             complement(5888..7831)
                     /gene="yidC"
                     /locus_tag="PK345_06395"
                     /inference="COORDINATES: protein motif:HMM:NF002356.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HPX34678.1"
                     /translation="MDKNTIIGLVLIFAIFIGFSILNQPSKEEIERRQRIQDSIAQVN
                     RQRDSLLKIKHAESEEIAEVEKREVDTTLSQEQLTLENLDRLGVFASSATGASEEYLI
                     ENEHLRIWISNVGGKIVNVELKEYKTFDSLPLVLFNNDDLIFGYTFFANSRRINTDKL
                     YFQPIFNNQEFSADREMMVSGSDSLLFAMRLYTDNPDGTPDKNRYIEYLYTVYGDDYM
                     IGYKLNLVGMDKVIDHGSGFIDLNWETEMRRQEKSLVNERNESTVYWKYDKESVKYLS
                     ERKDAKESLKLPVKWVSFKSRFFVATLIADQSFTNAEISSVTDPKNTDDHYLKNMSAL
                     VAVPFGNEANQSFDMNFYFGPNKYKTLKQYDLDLERQVPLGWSFFLIAWINIYAVINV
                     FNWLGDSGLNYGIVILILTVLLKLVLLPIAYKTYKSTAKMRILKPEIDEIAKKFPKPE
                     DAMKKQQATMTLYKKAGVNPMAGCVPQLLQFPILIAMFRFFPSSIELRQQSFLWAHDL
                     SSYDSILDLPFNIPFYGSHVSLFTLLMTISTIIYTKINNKLMGSSTGQMPGMKTMMYI
                     MPIMFLGIFNNFASALSYYYFLANIMTFAQMYLIRKTIDEEKIHQQILMQKTKPVKKS
                     KFQRKLEELSKQQQQIANSRKKR"
     gene            8129..10396
                     /locus_tag="PK345_06400"
     CDS             8129..10396
                     /locus_tag="PK345_06400"
                     /inference="COORDINATES: protein motif:HMM:NF015926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF349 domain-containing protein"
                     /protein_id="HPX34679.1"
                     /translation="MENKDQLNQIPDDFENETQDEKTSQLEDISAEATSSNEQGKDLL
                     SNTEPVEITEKIAPETSAESSDQEELTTFIEPQAEIVPPEPIEKEVEPITDILENKGD
                     DAIDIDKAQDDVVEQVVAEKVEEVKKDEIVEMAQDTKEEVTDVDKEAKVVVEDDQLDI
                     EPKDDEDIDDEDLEQEDEADDIKLNFEEMSREELISNLETFVNEEDITSVKSKIALIK
                     VAFLQKTKEFKESSYQERLNETEDKEEKQKIEPLVDDLETKFNELFDIYRQKRAIYTE
                     NLEKEKVDNLKKKQDILEKLKDLINSEETLKKTYDEFRELQEEWRNIGMVPKLEVNNL
                     WQNYHFLVEKFFDKVKINKELKDLDLKKNLEQKIELCEKVEELLLEPSIIKSFRELQR
                     LHEQWKEIGPVPDDKKDEIWERFKSTTDKINQRRRDHYKQLQEEQEANLAAKVALCEK
                     ADSLLAKEATSVKAWQERTRQFNDLFKMWKSIGPAPKKQNDEIWERFKTTIDAFYTEK
                     KEHFSKIKDQQINNYNLKLELCLQAEALKDSDDWRKSSRELIDLQKQWKEIGPVPRKH
                     AEKIWQRFRGACDEFFNRKSAHFSGIRIKEAENLKLKEELIASVENHNFGTDRAENLE
                     VLKEYQREWTEIGFVPFKEKDRLQNAFRNAINKRLDQLNISNSEIMLSTFMSRIDNIK
                     GTAEGNRQLQREQTFLQNKINQLREEIMLWENNIGFLADTKNANIVKSDFEKKIEKAK
                     SELSAMEAKLKYLTK"
     gene            10432..>11287
                     /gene="aroC"
                     /locus_tag="PK345_06405"
     CDS             10432..>11287
                     /gene="aroC"
                     /locus_tag="PK345_06405"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF013432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HPX34680.1"
                     /translation="MSGNTLGKIFSFTSFGESHGYAVGGVLDGFPAGVDIDLEYIQNE
                     LDKRKPGNSSPASARNESDKIQIVSGVFEGKSLGTPIAFFIFNENQKPEDYYALKDVF
                     RPSHADFTYYHKYSHVDYRGGGRASARETAARVAAGAFAKIFLNSVGVDITAFTIQVG
                     KIKMTKTAENIDKTTIDQSILKCPDPDISEKMMQQLEELKESGDTTGGVVECRITGCP
                     SGLGEPVFDKLSADLAKAMMSINAAKGFEIGSGFDAASMTGSAHNDKISKEKNGKIKF
                     LTNHAGGIL"
BASE COUNT         3539 a         2002 c         2274 g         3472 t
ORIGIN      
        1 gctcagattg gattaggcgt gccattggag aaaggaccag caatgcctga tgattatgta
       61 ctacctcctg ttatgttaat gtattctatt ggaggatata taccttttta agatattaat
      121 aaacctttta acatgaggaa agtagcaatt actttgacct tattggctgt gagaatgtcc
      181 tttacgtttg gacaaacaga tcctgattca atttcgataa aaaagttttg gcggttatca
      241 attttatttg agccccttac ttaatagaaa ataagagtag taagctgaaa atcctattag
      301 agtacaaatt atttgacaac tttttaaagt tttgaaatca tgcaataaaa attattttac
      361 tatttttaca ttcattatta taatttaaaa ttatccagct atgaaaaaaa cattgctgtt
      421 agtcgtttta ttatctgcct ttagtttggt gcacgctcag gaatacgtgc catttcccac
      481 ctccaatgtc tattggaatg agatcgaatt gtatcaaggt caatgtgatc cgccagagta
      541 ttgcaagaat accttttatt taatggggga cacagtaata gattcgtatt tatataaaaa
      601 aatatactcc aatgacggta attctacctc ttatgttggc ggattaagag aagaaaacaa
      661 acgtgtgttt ctctattaca cctggtgcga tcatagtgtt cttctttatg attttaattt
      721 gaatgttggc gatacaatgt ttatgtcgtg tctggccctt gaatgtgata ttaatgaaaa
      781 taattatatg acggtggtat ctgttgattc tgtattatta gaagaccaaa gctacagaag
      841 aagaataaat ttcgactctg gcccgcatat gtcatggatt gaaggtgttg gaagtgtttc
      901 aggactatta tatccgcact actcttgtat tttatgcatc tgttttcgcg aattgatctg
      961 ttttcaggaa gatgtcaaca ctttatattt gaatgaagaa aatgccgctt gttttaatta
     1021 ttgtgtatca gtgagtgatt tgaaaaataa tgataaaatg ttccaagttt atccaaatcc
     1081 ggccaaatat caaacttccg taacgatcaa atcaaatgag ggcattaata gagttgaatt
     1141 gtacgatttt ttaggcagaa tgatagcctg cgaacagaag ataaattcaa tggagtataa
     1201 actacaactt ggcaacctta gacaaggagt ctatttttta agagctaatt tacagaacag
     1261 ccaaatagtt agcaatcgcc tgataattaa ttaaataaac gaatgccaac agatgctggg
     1321 tatcctggcc gaacgctaaa acctttacca ggtaatgtgc cgcattagag cgttatcggt
     1381 cattgtaagc gacgagacac aattaaggta tgactgaaaa taataaaagg caacgacaag
     1441 caaaccttat aagagcattt aggaagattc acaggatgac aggagttttg ttgtttgtgt
     1501 ttttctttat caattcaata accggacttt tattaggttg gaaaaaacat agcggtggac
     1561 tattactgcc agaatcctat aaaggaacct ctactgaatt aaaagattgg ttaccactcg
     1621 acagtttaca caaaaatgcc tgtaaaatac ttcacgactc tgtttccact gacttatcgc
     1681 ttgaacttga aagaattgat gttcgtaagg acaaaggtat ggttaagttt gttttcaatg
     1741 accatttttg gggtattcaa cttgacggtg cgacaggtaa acttctacag attgaacgaa
     1801 gaaattcaga catgatagaa aacatacatg atggctctat tcttgaccat tatttgggaa
     1861 ctacgggaga gccaatcaaa gtagtttata caactattat gggcctgggg cttttgatat
     1921 ttacgataac cggtttttgg ctttggtatg gcccgaagcg aatgtgaaag aaaacaacga
     1981 aacgcaaata ttagctttgc gttagtaggc aatcagtttt tggtttcgca aacccaacaa
     2041 tgcaaagccc gggtaagtta ggtgaaaagg cagaatagcg tgctttgcaa cgaaaaaaag
     2101 aagacaagca aatgctatcc caataagcct gtaagattat aaatcgttcc ggatttatta
     2161 cctcctcaca ggaactggca cttcatacaa tctgtagttg gagagtctaa gaattttcca
     2221 actctggttt tcaaagccgt tttcgttatc agttataaac ttgtaattgc tataaagcag
     2281 cggaatgtca tggtagggga ggcaggtcat gaagttagag ccaaaattca ttagggcgct
     2341 aaggaagaaa attccagtat caaacccttt aaaggcgtag gcatccggat cggtgaaata
     2401 gtattcgcga aaacggcgaa caaacttttt aacctcttct gaatggtagt cggcaaattc
     2461 ggcggcaggg aaatgtgtat tgagatttcc caaatggtta aaatcaagtc cctccatttc
     2521 gagccagtca gggagtccaa tcactgtaat atcgtatctg ttcctaatga ggttcagttt
     2581 tcgcaaaaga tcgagtataa ccaccttgtt ttcggaatag acaatgatta cattgggtct
     2641 ctctttcaaa gcttctctat tgaaaacact tagactgtcg ttctgaatat taaactcaac
     2701 gatttgctgc catctgtttt ctaagttttc aaaaagcgct gatctaagat taattatatg
     2761 ctcttcatcg cgcattttac tttggcgggc aataaagatt tttgagtcgc tgagtttgta
     2821 tgaaataaat tcggcaattg tattgtaaat tctcttttca tttggaataa ccttaattac
     2881 aaaaggatta tcattaatga catcgctttt tgctgagagc ggatttataa tggggatttt
     2941 gtgcttaagg gcaaactcag ccacgatccg gaagcttgaa ttaaaaaatg gccctacgat
     3001 cagatccatc ttttttagag caggattttt aagacattgt tgtgcctggc tgacatcttc
     3061 ttcaacatta aacacgtgaa tacgtgcttc taatcctact ttttcgagcg agtcaacagc
     3121 aagtctaaag ccttcataaa atttgatgaa ttgaaacgat ttgctatttg gaattttgtc
     3181 ttgcttggat gtcgaaggtg tagttattgt tttctcaacc aattcatcga aataaagagg
     3241 tatcaatagc gcaatatcaa aggtttgtgc tgcaggtatc tgtgcacaaa ggtcttttga
     3301 tgttactgga ggggtatatg gttttttatt ctcttcaatc gtgtaaatct cgggtgggag
     3361 agtaatttcc gattcgatat ttttcaatgg cggcaacggt attttaattg tctgtcccgg
     3421 ttgcaactgc ctggaaatat aagggttggc tttttgtatt tcttcttgcg acagcccata
     3481 gtttcgagcg aggcggttaa ttgtaattcg ctgtgcgata ttatgtgtta taaaaccttt
     3541 cttagtcggg ttattcggga ttttgattat ctcacctttt gaaagttgtt cacccaaacc
     3601 ggggtttaaa aacagtatgc tgtcaacact tacgcgatac cgtattgcca attcataaat
     3661 cgtctcgtgg tttctttcct gaaattcaaa atatcctttt tccggctttc cttgtccacc
     3721 gttttcataa gtttttgttt gcggttctgt ttttttattc gtggattcaa agtcgggtat
     3781 tattatctcc atcccttcaa ttaatccatc ttcaagtccg gtattcgcag ctcgtatggc
     3841 ttctatagag atgttgtatt ttttggcaag gccatacaat gtttcgcctt tggctacttg
     3901 atgtttgtgt aaattatttg actctgagcc aaattgaatg gaatgtatgc ggtgagttgg
     3961 aatatagatt ttttggcctg acgaaagcgt attctcaagc tcaggattgg cctcatatat
     4021 atctcttaca tcaacattgt aggttttgga aattgaaaat agagtttgac ctttttccac
     4081 tgtatgtagg taatactcct tgccattaat atttacaatt ttttccgact tttttattag
     4141 tatctgtgca ttagcccatg atgatatgat aagaaaaatg gtcaggaaaa ggatcgtttt
     4201 aatgttcatt ttattttttg atgagatatc taactttctt aaagggagta aatagaacaa
     4261 tttttttaca gttttatttt tagcatgttt tactgaaagc tctgaaatcg tcataataag
     4321 attaagattt acaattgagt ttcaactatt cccattcaat agtagccgga ggttttgaac
     4381 taatatcata cacaaccctg tttatccctt ttacattcct gataatttct tcggagactt
     4441 tttccaggaa gtcgtagggc agatgtgccc attcagcggt cattccgtcg gttgagtgta
     4501 ctgctctcaa ggcaatggca ttttcgtaag tcctcttttc gttcgatact cctaccgatt
     4561 gaacaggaag caagatggca agagcttgcc acaccttatc atataggccg cactctttta
     4621 actttttaat gaaaatatag tcggcttcct gcaaaatttt tattcgttcg gctgtaatct
     4681 ctccaagaac cctcacaccc aagccagggc ctgggaaggg atgcctattc aggatttcgg
     4741 gttttaaacc aagctcaaag cctacgcgac gtatctcatc tttaaagagg tttttaagag
     4801 gctccacaat ctttagtttc atataattgg gcaggccgcc tacattatga tgtgatttta
     4861 tggttgcagt ggatcctggt attggcgccg attcaattat atcgggataa atagtgccct
     4921 gcccaagcca tctcacctcc tttatcttgt gagcctcatc atcaaaaatc tcaataaata
     4981 tatgcccgat agcctttctc tttttctcag gatcagtaat gccggccaat gctttgtaaa
     5041 aacggtcggc agcttgtaca ccttttatgt ttagtccaat atcatgataa gactcaagca
     5101 cggaggcgaa ttcatttttc cttagtaatc cgttatctac aaaaatacag tgcaggtttt
     5161 tgccaatagc cttgtgcagt aacattgcag ctactgagga atctacgccg cccgaaagtc
     5221 ccaatacaac cttgtcatct tgtaattgat ctttaagaga ttttgtagag tcatgaataa
     5281 aacgtgcagg cgtccaatcc tgacgacatt gacaaatgga tacaacgaaa ttttcgagca
     5341 actgaactcc atcggcagtt tgatcggcta caggatgaaa ctgaatacca aatatattcc
     5401 tactgttgac ttgaaatccg actatttccg aggtttcagt ccgcattatg attttattat
     5461 tatcagggat tgacaatatt accgatccgc ctgccgacaa tacctgacta cccaatttaa
     5521 ttgattgaac caacgtattg gatggatcaa tatctccgaa atttgcgatt acatttttcg
     5581 aagtagcgga ttttttaatt tgtccgccga ggtgctgtgc gaggcgttgc gcgctataac
     5641 caattgcaag aattggaata tcagctttaa aaagttgatc gaaatcaggt tttggcgaat
     5701 tgggatcgtc aattgagtat gagctatccg aaaggattat cccttttatg tttttattcg
     5761 taggaatctt gttgtacgga tagatttcgc aatatacctt tagctcgcgt gttttacgag
     5821 ctatcatttg ggtaaaatta gagccgaagt caactatcaa aattatttca agcatgtggt
     5881 gcggttatta tctctttttt cgcgaatttg caatctgttg ctgttgcttt gaaagctctt
     5941 ccagtttccg ttgaaatttt gattttttca ccggtttagt tttttgcatc aatatctgtt
     6001 ggtgaatctt ctcttcgtct attgttttcc gtatcaaata catctgggca aacgtcataa
     6061 tgttggcaag gaagtaataa tagctcaagg cggaggcaaa attattgaaa ataccaagaa
     6121 acattattgg catgatatac atcatggttt tcatgccggg catctgtccg gttgacgacc
     6181 ccatcagctt gttgtttatt tttgtgtaaa tgatagtcga aatagtcatg aggagggtaa
     6241 aaaggctgac gtgcgatccg taaaaaggga tgttgaaagg gagatcaagt atcgaatcgt
     6301 aacttgatag gtcgtgtgcc cataggaacg actgctgacg caactcgata gatgatggga
     6361 aaaatctgaa catcgcaatg agtatcggga attgcaacaa ctgcggcaca cagcccgcca
     6421 ttgggtttac gccggccttt ttataaagcg tcattgtagc ctgttgtttt ttcattgcat
     6481 cttcgggctt ggggaatttc tttgctatct catcaatttc tggcttcaaa atgcgcatct
     6541 tggccgttga tttataggtc ttgtatgcaa taggaagtaa tactaatttc agtaaaacag
     6601 taagtatcaa aatcactatt ccataattca gtcccgaatc gcccaaccaa ttgaaaacat
     6661 taattactgc gtagatatta atccaggcta taagaaaaaa actccaccct aaaggcactt
     6721 gtctttcaag gtctaaatca tattgtttta gagttttgta tttgtttggg ccaaaataaa
     6781 aattcatgtc gaaactttgg ttagcctcgt taccaaatgg aacagcaact aaagccgaca
     6841 tattttttaa ataatgatcg tccgtatttt ttggatctgt aacggatgaa atctcagcat
     6901 tggtaaaact ctgatcagct attaatgtag caacaaaaaa ccttgatttg aacgaaaccc
     6961 actttaccgg cagtttaagt gattcttttg catccttacg ttctgagagg tatttcaccg
     7021 attccttatc atacttccaa tatactgtag attcattacg ttcgtttaca aggcttttct
     7081 cctgcctacg catctcagtc tcccagttca gatcaataaa accgcttcca tggtctatca
     7141 ccttatccat accaacgagg ttgagcttgt aaccaatcat gtaatcatca ccataaacgg
     7201 tatagaggta ttcaatatac ctgtttttgt caggtgtgcc atcaggattg tcggtgtaga
     7261 ggcgcattgc aaaaagaagc gaatcgctgc cagaaaccat catctcccga tcagcggaaa
     7321 attcctgatt gttgaaaatg ggttgaaaat aaagtttgtc ggtattgatt ctacggctat
     7381 tggcaaaaaa agtatagcca aaaatcagat catcattatt gaaaagaaca agtggcaaag
     7441 agtcgaaagt cttatattcc tttaattcta cgttaacaat ttttccgcct acatttgaaa
     7501 tccagattct gagatgttcg ttttcaataa gatactcttc agaggcgcca gtagctgatg
     7561 atgcaaacac acctaatcta tcaaggtttt caagcgtcaa ctgttcttgc gaaagagtcg
     7621 tgtcaacttc acgcttttct acttcagcaa tctcctccga ttcggcatgt tttattttaa
     7681 gtaaagaatc tcgctgccta tttacttgag ctatcgagtc ctgaatccgt tgtcttcgtt
     7741 caatctcctc tttcgacggt tgatttaaaa ttgaaaaacc aatgaatatt gcaaaaatca
     7801 ataccaaccc aataatagta ttcttgtcca tcagaccact tttaatcgtt atttttaaag
     7861 tgagcaaaga tatgattttt ggataagctt atagtttaga actactttcg caataacatg
     7921 ttgctgttca aacattgttg gggctttact ttcaaattaa gatgattttt aaaattaaaa
     7981 ttgttatcgt cggaaatttt cttttagaga aaaaatgatc cttataaatt attgctattt
     8041 ttgcgactct tttttttaag ttaatttcta ataatcagtt ttttacattg taaaaagcca
     8101 gaacacacac aacaattttt taaattttat ggaaaacaaa gatcaactta atcaaattcc
     8161 tgatgatttc gaaaacgaaa ctcaggatga gaagacttca cagttagagg atattagcgc
     8221 tgaagcaact tcatcaaatg aacaaggtaa ggatttacta tctaacacag aacctgttga
     8281 aattaccgaa aagattgccc ccgaaacttc tgctgaatca tcggatcagg aagaattaac
     8341 aacttttatc gagccgcagg ccgaaatcgt acctccagaa ccaatcgaaa aagaggttga
     8401 gccaattaca gatattttgg aaaataaggg cgatgatgcc attgacattg acaaagctca
     8461 ggacgatgta gtcgagcaag tggttgcaga aaaagttgaa gaagtaaaaa aagatgaaat
     8521 agtggagatg gcgcaagata caaaagaaga ggttactgat gtagataagg aagctaaggt
     8581 agtagttgag gatgatcagc ttgatattga gcctaaagat gatgaagata ttgacgatga
     8641 agatttggaa caagaggatg aggctgacga tattaagctt aacttcgaag agatgtcccg
     8701 tgaagagttg atcagtaatc ttgaaacctt tgttaacgaa gaggatatca caagcgtaaa
     8761 aagtaaaatc gcacttataa aagttgcctt tttacagaag acaaaagagt ttaaagagag
     8821 tagttatcag gaaagactta atgaaacaga ggataaagaa gaaaaacaga aaatagagcc
     8881 tcttgttgat gatcttgaga caaaattcaa tgagttattt gatatttatc gtcagaaaag
     8941 agcgatatat accgagaatc ttgaaaaaga gaaagttgat aacctgaaaa agaaacagga
     9001 tatccttgaa aagctaaagg atttgatcaa ctccgaagag actcttaaaa agacttatga
     9061 tgagttcagg gagttgcagg aagagtggag aaatataggg atggttccaa aattggaagt
     9121 taacaaccta tggcagaact atcacttttt agttgagaag ttttttgata aagttaagat
     9181 caacaaggag ttaaaagacc ttgatctcaa gaaaaatctg gagcaaaaaa tagaactttg
     9241 cgaaaaagtc gaagagttgc ttcttgagcc ttccataatt aagtcgttta gggagttgca
     9301 acggctccat gaacagtgga aggagattgg tcctgtacct gatgataaga aggatgagat
     9361 ctgggaacgt ttcaaatcta ctacggataa gataaatcag cgccggcgcg atcattataa
     9421 gcaactgcaa gaggagcagg aagccaatct ggctgcaaaa gttgcacttt gcgaaaaggc
     9481 tgatagcctt cttgccaaag aggctacatc agtaaaagcg tggcaggaac gcacaagaca
     9541 attcaacgat ctgtttaaaa tgtggaaaag cattgggcca gcgcctaaaa agcaaaacga
     9601 tgaaatatgg gaacgcttta aaacaaccat agatgcattt tatacagaga agaaggagca
     9661 tttttccaaa ataaaggacc aacagataaa taattataac cttaaacttg agctttgcct
     9721 tcaggctgaa gcattgaaag attctgatga ctggcgaaaa tcatcacgtg agttgattga
     9781 tttgcaaaaa caatggaaag agataggccc tgtaccacgt aaacatgctg aaaaaatatg
     9841 gcagcgattc aggggtgcgt gcgacgagtt ctttaataga aaatcagctc atttttccgg
     9901 aataagaatt aaagaagccg aaaatctcaa gcttaaagaa gagcttatcg cctccgtaga
     9961 aaatcataac tttggcaccg acagagcaga aaacctcgaa gtacttaaag aatatcaaag
    10021 agaatggaca gaaattggat ttgtgccttt taaagaaaaa gaccgtttgc aaaatgcttt
    10081 tcgtaacgca ataaataaac gattagatca gctcaatatc agcaattctg agattatgct
    10141 ctccactttt atgtcgcgga ttgataatat caaaggaaca gccgaaggaa acagacaact
    10201 gcaacgcgag cagacctttt tgcaaaataa gataaaccaa ttgcgtgaag agattatgtt
    10261 gtgggagaat aatatcggtt ttcttgccga tactaaaaat gccaatattg ttaaatccga
    10321 tttcgagaaa aagattgaaa aggcaaagag cgaattgagc gcaatggaag ccaaacttaa
    10381 atatttgacg aaatagccat ttgttttttt ctggaattaa atttctgacc catgtcgggc
    10441 aatactttag ggaaaatttt cagcttcacc tcttttggcg aatctcatgg ctatgctgtt
    10501 ggcggagtac ttgatggctt ccccgcaggt gttgatattg atttagaata tattcaaaat
    10561 gagctcgaca aacgaaaacc cggaaattct tcacctgcct cagctcgaaa tgaatccgat
    10621 aagatacaaa tcgtgtcggg tgttttcgaa ggcaaatcgc ttggaacgcc aatagccttt
    10681 tttattttca atgaaaacca aaaaccggag gattactatg ccttaaaaga tgtctttcgg
    10741 ccatctcatg ctgattttac ttattatcat aagtatagcc atgtagatta caggggaggg
    10801 ggtagggcgt cggcgaggga aacagctgca agagtggctg ccggcgcttt tgctaagata
    10861 tttttgaata gcgttggggt tgatattacc gcttttacca tacaggtcgg taaaataaag
    10921 atgacaaaaa ctgctgaaaa tattgacaaa acgacaatag atcaatctat actaaaatgc
    10981 cctgatcctg atatctcaga aaaaatgatg cagcaactcg aagaattaaa ggaatccggc
    11041 gataccaccg gtggtgttgt ggaatgccgt attacaggat gcccgtcagg attgggcgaa
    11101 ccagtattcg acaaactctc agccgatttg gcaaaagcca tgatgagcat taatgccgct
    11161 aaagggtttg aaataggatc gggatttgat gctgcttcca tgacagggtc ggcccataat
    11221 gataaaatat ccaaagagaa gaatgggaaa atcaaatttt taaccaacca cgccggcggt
    11281 atcttag
//
LOCUS       DAOOAI010000076        11142 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_7440_length_11142_cov_4.664111, whole genome shotgun sequence.
ACCESSION   DAOOAI010000076 DAOOAI010000000
VERSION     DAOOAI010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11142
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7440_length_11142_cov_4.664111"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2213)
                     /locus_tag="PK345_06410"
     CDS             complement(<1..2213)
                     /locus_tag="PK345_06410"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPX34681.1"
                     /translation="MLKHLLLTIVSLLAINVMLFSQTGSIQGKVLDVETNEPIPFANV
                     AIFSGGSLLTGATTDFDGKYAVRSLRPGSYSVRASFIGYQTKEVAGVIVRADQITFEN
                     ISLKSTTEVLETVEVVDYKVPLISKDQTTSGATLTSEEIEKMPNRSATSIATTVGGVY
                     SDADGGSISIRGQREEGTVYYIDGMRVLGSASMPESSIEQVSVILGGVPARFGDATGG
                     IISLTTRGPSSEFGGGLELQTSEFLDRFGYNRLGFSLQGPILKKDMGTSKSSVLGFFV
                     AGDVVYRKDGRPAVRGTLDKITDEYLAYLKENPLRPSGTGFGTFDNSLFTRRNNTESI
                     HSSLNTESLNVSLSGKIDIRVSPTINMSIGGSYNLQDYKAFQYAGSLFNWENNPHVYN
                     NTWRLYGRLTQRFPTDRESGSAIKNVFYTIQADYSKFYQVVENESHRDNIFNYGYLGK
                     YETYKRRSYELGTDTVNGHIYTNVYVQNAFQDTAFTFQPYDMNPVVARYTEQYYEFYP
                     EKGDWRNPSSGYWLNANQVQLYGGLLNGQSPDNVYTMWQGVGTQYNGYSVSDNSQIGI
                     SANASADIGNHAIEFGLQYEQRTNAYYGYGPVGLWTMMRQIANAHINELDKSSPHLLM
                     VDGVFQDTINYDRLYSFTDQRIFDINLRKKLGLDLQGTDWIDIDSYDPNSKTINYFDA
                     NGVLHKNASLGDGFTIDMFSPDELFNDGYSYVGFYGYDPYGNKLKGRPAFDDFFN"
     gene            complement(2328..2840)
                     /locus_tag="PK345_06415"
     CDS             complement(2328..2840)
                     /locus_tag="PK345_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34682.1"
                     /translation="MEDLFYFLLLVAWLVFSFYQQNQKKRRKEAAKAAAERAKVDEMR
                     KITQEVQQEKAEEESPGKVIETIFKTLLDDDTETSLEEETIVEPVVNYDEDIIEEKNI
                     YQRYFESKFSKDQKILDKEFESHDKITPTIPVTIEEDYEKFDLAETIVQSDFDLRSAV
                     IYSEVLKRKF"
     gene            3509..3964
                     /locus_tag="PK345_06420"
     CDS             3509..3964
                     /locus_tag="PK345_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34683.1"
                     /translation="MKQRTNFLSRLFLAALVVCFFFIGGCGKDEQPPIDVGYVNLTIY
                     PNSTEYIQLNSPGGYLYLYAELPSRGIIAYRLSSNEFKAYERTPTYKKDSCGIYNRVI
                     VDESGFFIIDTCTNSKWLIIDGTVYEGPAKYALYPYKTFYDGYTLRIYN"
     gene            complement(4100..5527)
                     /gene="ahcY"
                     /locus_tag="PK345_06425"
     CDS             complement(4100..5527)
                     /gene="ahcY"
                     /locus_tag="PK345_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HPX34684.1"
                     /translation="MEKLMIDTNLDYKVKDINLAEWGRKEIEIAEKEMPGLMAIRRQY
                     SEEKPLEGVRISGSLHMTIQTAVLIETLTELGADVRWSSCNIFSTQDHAAAAVASRGV
                     PVFAWKGETIDEYWWCTAQALSFPGGKGPQLVVDDGGDATLLLHKGVAVEKNPELLKA
                     QTSNAEERAIMGLLQRTFVDDPKKWHRTTEEWLGVSEETTTGVNRLYQMKERNELLVP
                     AINVNDSVTKSKFDNLYGCRESLADGIKRATDVMLAGKVVVVLGYGDVGKGSAKSMRA
                     YGARVIVTEIDPINAMQASMEGFEVTTIDEALKEGQVFVTATGNKDVITLDHMKKMKD
                     EAIVCNIGHFDNEIQVEKLNSDPTVRKINIKPQVDRYTFDDGHSIYLLAEGRLVNLGC
                     ATGHPSFVMSNSFSNQTLAQIDLWKNRNNYKVDVYRLPKIIDEAVARLHLDHLGVKLT
                     KLTQEQADYIGVPIGGPYKPEHYKY"
     gene            5670..6347
                     /locus_tag="PK345_06430"
     CDS             5670..6347
                     /locus_tag="PK345_06430"
                     /inference="COORDINATES: protein motif:HMM:NF014626.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0018142 - protein-DNA covalent
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
                     /protein_id="HPX34685.1"
                     /translation="MCGRYSFAPDLKIVNEHYDISVNDGDLTPNYNCAPSQLLPVITN
                     DKSIGFNFFRWGLIPFWAKDISIGNKLINTRSETILEKPSFRNAFRQRRCLVPADAFY
                     EWKQEVKEKIPYRIFLKNQNIFSMAGIWEKCKLSNGETIFSFAIITTQPNTLMTKIHN
                     RMPVILDKKGEDLWINNTDEKELTNLLKPFPAEQMTAYRISNLVNSPRNNSPKIIEPI
                     ADNSLFN"
     gene            6421..8913
                     /gene="priA"
                     /locus_tag="PK345_06435"
     CDS             6421..8913
                     /gene="priA"
                     /locus_tag="PK345_06435"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HPX34686.1"
                     /translation="MDDLFAEVLLPLPLSGSFTYKIPSGLDVEVEPGKRVVVQFGKSK
                     LYTGLVKTLHHNKPENYTAKPIISVLDHTPVVNPIQFHFWDWMADYYMCFEGEVMNAA
                     LPSALKLASETRIVQNPSFNGDTSQLNNTEYLVAEAIDIQKSLTISEVSRIVEFKKVI
                     PLIKNLIEKNVVLLEEELKEVYRPKMETWMRLTEKYRTEEAIKTLMDSLEKRAYKQLE
                     VMISFLSNIDDFSDENAMIKKSDLLQLASADTQQITALERKGVFEIIEKAGSRLESFE
                     VTTPVSSIILTEPQQTAFSHIKQGFENHNVTLLHGVTSSGKTEIYIRLIDEVINSGKQ
                     ALFLLPEIALTTQLINRVRKYFGNQVGVYHSRYSMMERVEIWNKVIQEYDSLTDATDK
                     YSVILGARSALFLPFNNLGLIIVDEEHDGSYKQFSPAPRYSARDSAIVLAQMHNARVL
                     LGSATPSIESYFNAKSGKYALATLNERYGGMELPEIIISDLKIETRRKTMKSHFSSLL
                     INLIEQALENKEQIILFQNRRGYSLRIECGMCQWTPCCKNCDVTLIYHKQNNQLKCHY
                     CGYSIPIPAVCPECGYTGLHMKGFGTEKIEDELALLFPKASITRMDLDTTRTKSSYQN
                     IISNFEQRKIDILVGTQMVTKGLDFDNVSVVGILNADNLLSYPDFRSFERSFQLMAQV
                     SGRAGRKFKRGKVIIQSYRPDHEVIKYVVYNRYDLLYQNQIAERRRFKYPPIFRLIEL
                     QLQHKQEQKVSEAAYELASLLKQKLGNRVIGPEFPIVSKIKGLYLKNILIKLEKTKHT
                     SQLKEDVKELLDYFTKNAKHKSVRVIVDVDPY"
     gene            8977..10383
                     /gene="xseA"
                     /locus_tag="PK345_06440"
     CDS             8977..10383
                     /gene="xseA"
                     /locus_tag="PK345_06440"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HPX34687.1"
                     /translation="MSEYFKGHKVFSLLEITQSIQRAFQKWYSDTYWIKAEMNKLNYY
                     KYSGHCYPDLLERKQEKIVAQIRATLWDIDYQRINSTFLKVLNEPLKDGINILFLAKI
                     SFTPLHGLSLSILDIDPSYTLGDLEREKLNTINRLKSSNIFDQNKRLQASPLIQRLAI
                     ISVETSKGLSDFMKIINDNRWGYRFFTMLFPALLQGDKAPESIMGQLRRINRVKHHFD
                     AVAIIRGGGGDIGLTSYNNFQLAHAVATFPLPVLTGIGHSTNETVTEMVAHINAITPT
                     ELADFLIQRYRNFDIPVTEAAKRIKLLSQQKISSAEQKLKNEMRFFKSVAEKKITTQS
                     EVILSKIQQIRQNIRSEYKVQLSKLEALKLNLNKHTPSAYKNEFDKIRNLKLNLKKNS
                     GILLKNFSNEILSIENQLRALHPDNVLKRGFSITLLNGKALTKANNASVGDEVETILF
                     KGAIKSKITHKSINKDHG"
     gene            10376..10603
                     /gene="xseB"
                     /locus_tag="PK345_06445"
     CDS             10376..10603
                     /gene="xseB"
                     /locus_tag="PK345_06445"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014225608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HPX34688.1"
                     /translation="MAKQLTYIEAFEELQQIVNDIEEGETGIDQLSEKVKRAAELIKI
                     CKNKLISTESDVNKILDELEETSTNSVEAET"
     gene            complement(10647..>11142)
                     /locus_tag="PK345_06450"
     CDS             complement(10647..>11142)
                     /locus_tag="PK345_06450"
                     /inference="COORDINATES: protein motif:HMM:NF025397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34689.1"
                     /translation="NPEISIGDTPAVSLCRNFEDVDEIRPGNFVFYDLIQQELGVCRY
                     DQIAVALACPIVAVHVERLEVVIWGGAAHLSKEALNDEGGGKVFGKVVLLDKFGWTLP
                     LDGTFVKSVSQEHGIIKTTPEHIDKFSPGVFIGILPVHSCLTTIMIKEILTLNGEVIH
                     TMNC"
BASE COUNT         3573 a         2289 c         1929 g         3351 t
ORIGIN      
        1 ttattgaaaa agtcatcaaa tgcagggcgt ccttttaatt tatttccgta aggatcatat
       61 ccataaaagc ctacataact atagccgtca ttaaacaatt catcgggcga aaacatgtca
      121 attgtaaagc cgtcgcctaa agatgcattt ttatgtaaaa cgccgtttgc atcaaaataa
      181 ttgatggttt tgctattagg atcatatgag tcaatatcaa tccaatctgt tccttgaaga
      241 tcaagaccaa gttttttcct tagattaatg tcaaaaatcc tctgatcggt aaaactatat
      301 aggcgatcat aattaatggt gtcctggaat actccatcaa ccatcagaag atgggggctg
      361 cttttgtcaa gctcgtttat atgtgcattg gcaatctgac gcatcatagt ccaaagtcct
      421 acaggcccat aaccatagta agcattagta cgttgctcat attgtaatcc aaattcaatt
      481 gcatggttac caatatcggc tgaagcatta gctgaaatac ctatttgcga attatcagaa
      541 acggaataac cattatactg agtgccaaca ccctgccaca tagtataaac attatcagga
      601 gattgtccat ttagcaatcc accgtaaagt tgtacctgat ttgcatttaa ccaatatccg
      661 cttgaaggat ttcgccaatc tcctttttca ggataaaatt catagtattg ctccgtatag
      721 cgtgcaacca cagggttcat atcataaggc tgaaaggtaa aagctgtgtc ttgaaaagca
      781 ttctgaacat aaacattggt ataaatatga ccattaacgg tatctgttcc taattcgtag
      841 gatctacgtt tataagtctc gtactttcca aggtaaccat agttaaaaat gttgtcgcga
      901 tgtgattcat tttcaacaac ctgataaaac ttagaatagt ctgcctgaat agtataaaaa
      961 acgtttttaa tggccgatcc actttcccta tcagttggga agcgttgagt caatcttcca
     1021 tataatctcc acgtattatt gtaaacatga gggttgtttt cccaattaaa caatgaacct
     1081 gcatattgga atgccttata atcctgcaag ttatatgagc cgccaataga catatttatt
     1141 gtaggagaaa ccctaatatc aattttacct gataagctta catttaaaga ctctgtattt
     1201 agtgatgaat ggattgattc ggtattattt ctcctcgtaa agaggctatt atcaaatgtt
     1261 ccgaatcctg ttcctgacgg tcttaatggg ttttctttaa gataagcaag gtattcgtcg
     1321 gttattttat ccagtgtacc cctaacagcc ggtctgccat ctttgcgata aaccacatct
     1381 cctgcaacaa agaatcctaa tacagaagat ttcgacgttc ccatgtcttt ctttaaaatt
     1441 ggaccttgca aactaaagcc gaggcggtta tatccgaatc tgtcgaggaa ttcggaagtt
     1501 tgaagttcaa gcccgccacc aaattcgctg gacggccctc tggttgtcaa gctgataata
     1561 ccacctgtag catctccgaa cctggccggc actcctccaa gaataaccga tacttgttca
     1621 attgatgact caggcatact agcagagcct aacacacgca ttccatcaat ataatacacc
     1681 gttccctcct ctctttgtcc tcgaatacta atgcttcctc cgtcagcatc tgaataaaca
     1741 cctccgacag ttgtagcaat tgaagttgct gatcggttag gcatcttctc aatctcttcc
     1801 gaggtcaagg tagcgcccga ggttgtctgg tctttggaaa taagcggcac cttataatcc
     1861 acaacttcca ccgtctcaag aacctcagta gtagatttca atgaaatgtt ctcgaaagtg
     1921 atctgatcgg cgcgaacaat aacaccggca acttctttgg tttgatatcc aataaaagaa
     1981 gccctgacag agtagctacc aggccttaac gacctaacgg cgtacttgcc gtcaaaatct
     2041 gttgttgctc cggtgaggag gctaccccca gaaaaaatgg ctacgttggc aaaaggaatt
     2101 ggctcgtttg tttcaacatc cagaacctta ccttgaatgg atcccgtttg agaaaacagc
     2161 atcacgttaa ttgccaacaa actcacaatt gttaaaagta aatgttttaa cataccatat
     2221 aattttatat aagattttac attttactaa aggcggctaa attagtaata tttaaatcgt
     2281 acaaacaaac cttttcagtt tttactatta tttaaattca aactgaatta aaattttcgt
     2341 tttaaaacct ccgaatagat aactgcacta cgtaaatcaa aatcagactg tacaatagtt
     2401 tcagctaaat caaatttttc ataatcctct tcaattgtta caggaattgt gggagtgatt
     2461 ttatcatgac tttcaaattc tttatccaat attttttgat cttttgaaaa ttttgactca
     2521 aaataccttt gataaatgtt tttttcttca atgatatctt catcataatt aacaacaggt
     2581 tcaacaatgg tttcctcttc taatgaagtt tcagtatcgt catccaacaa agttttaaaa
     2641 atagtttcga taactttccc tggcgactcc tcttctgctt tttcttgctg cacctcctgt
     2701 gttatttttc gcatttcatc aactttcgca cgttcagccg cagcttttgc agcctccttt
     2761 cgtctctttt tctgattttg ctgataaaat gaaaagacta accaagcaac caacaacaaa
     2821 aagtaaaaca agtcttccat attcctacat ttttcagatc attagtcctt taaaaccttg
     2881 caaaaataat gtttgattat taataagctt taattttttt gtaaaaatag atatttcggg
     2941 ctaatgcact aaaaaaactt acttaaaact tttaatatgg caagattgta tcctctatga
     3001 agtattcata atcagaacaa taaaaaacct actaaacaaa gactcaataa ccgtatcatt
     3061 tcccaacaat tttgaaaaat taagatatta aaatgttgtt caagaaaaat ttaggtaacc
     3121 tttaattgat taaagcggcc aaaaattcat ttgcgaagac ttttctgata attcgtttgt
     3181 cttttttgcg aagctttcat tctttgttgc gtctctttag tctgcctttg aaaatgctgt
     3241 ttttttaatg ctttataggc ttttttcctt ctccgttcct cttcgactaa ttttttctca
     3301 attctatttt gtttccttat tgcctgagtt ttacaacctt cggcagttat taaaactaac
     3361 aaaacgctga taagaaaaat taaattttta ccccttagca tatcaaatca gtcgaaatat
     3421 taaatccata ttttcgtttt cattattgat cagaatcagt ttcggtaagt aagaaaatga
     3481 tagatttaag aaactcaaat acttttagat gaaacaaaga acgaattttc tttcccgatt
     3541 atttttggcc gctctcgttg tttgtttttt ctttattggc ggatgcggaa aggatgagca
     3601 accacctatt gatgtgggct atgtaaatct cactatttat cccaattcca ccgaatatat
     3661 acaattgaat tcgcctggag gatatcttta tctttatgcc gagctaccaa gcagaggtat
     3721 tattgcctat cggctttctt ctaatgaatt caaggcttat gagcgaactc ccacgtacaa
     3781 aaaggatagt tgtggaatat acaaccgagt gattgttgat gaatcgggct tttttataat
     3841 tgatacttgt accaacagca agtggttgat cattgatggt actgtttacg aagggccggc
     3901 aaaatatgca ctctacccat acaaaacctt ttatgatggc tataccctgc gcatttacaa
     3961 ttagtttatc agaatccttt caaacacaat cgccgcctcc attgattgca taaatagtgc
     4021 agatatagaa aatcaaataa aagcttaatt agtttacgtt tacttgttgg agattcttaa
     4081 aaagaataag atctgttaat caatatttat aatgctcagg cttgtaaggc ccaccaatcg
     4141 gcactcctat ataatctgct tgttcctggg taagttttgt aagcttcact ccaagatgat
     4201 cgaggtggag cctggcaacc gcttcatcaa tgattttcgg taagcggtaa acatctactt
     4261 tatagttgtt tctgtttttc cataaatcaa tctgcgccaa ggtctgattg ctgaatgagt
     4321 tactcatcac aaacgatgga tgtcctgtgg cacaaccaag gttaactaat cgcccttcgg
     4381 ccaacagata aatagaatgt ccatcgtcaa aagtatatcg gtcaacttgt ggtttgatat
     4441 taatttttct tactgtaggg tcgctgttaa gcttttctac ctgaatttcg ttatcaaagt
     4501 gaccaatgtt gcaaacaatt gcttcatctt tcatcttttt catgtgatca agcgttatca
     4561 catccttatt tccggtagcg gtaacaaaaa cttgtccctc tttaagagct tcatcaatag
     4621 tagttacctc aaaaccctcc attgaagcct gcatggcatt aattggatct atttcggtaa
     4681 ctatcactcg agcgccataa gcccgcattg attttgccga tcctttccct acatcgccat
     4741 atcctaacac taccactacc tttcctgcaa gcattacatc agtagcccgc ttaattccat
     4801 cggctaacga ctcccgacag ccatataaat tatcgaattt tgatttggtt accgaatcat
     4861 tcacattgat cgccggcaca agtagttcgt tacgctcttt catctggtaa agcctgttga
     4921 cacccgttgt tgtctcttcc gacacaccta accactcttc tgtcgttcga tgccattttt
     4981 ttgggtcatc cacaaaagta cgttgtaaca gacccattat tgctctttct tcggcatttg
     5041 atgtttgtgc tttcagcagt tccggattct tttcaacggc tactcccttg tgcaatagca
     5101 atgtggcatc tccaccgtca tccaccacca attgaggccc tttaccgccc ggaaaggata
     5161 gcgcttgagc agtacaccac caatattcat caatggtttc acctttccat gcaaataccg
     5221 gtactccacg tgaagcaaca gccgcggcag catggtcttg ggttgaaaaa atattgcagc
     5281 tcgaccatcg tacgtcagcg ccaagctcag ttaaagtctc aatgagcaca gcagtctgta
     5341 tggtcatgtg taacgaaccg ctaatacgta caccttctaa aggcttctct tctgaatact
     5401 gcctcctgat tgccatcaaa cccggcatct ccttttcagc aatctcaatc tctttcctac
     5461 cccactcagc cagattaata tctttcacct tataatcaag gttggtatct atcattaatt
     5521 tttccatatc ttaccttcga tttttttgca aaaatatagt tttcatcctt taacataagt
     5581 aaaaattggt tgttcatctg tcggtataaa atataaccat taaaggaagc tttttatttt
     5641 tgtaaattgt tttcttaaac ataatacaaa tgtgtggcag atattctttt gctccggatt
     5701 taaaaatcgt aaacgagcat tatgacatta gcgtaaacga cggcgattta acaccaaact
     5761 acaactgtgc tccttctcaa cttttaccgg tgatcacaaa cgataaatca ataggtttca
     5821 acttttttcg ctgggggctt attccatttt gggcaaaaga tatctctatt ggcaacaagc
     5881 ttatcaatac aaggagcgag accattttag aaaaaccatc attcagaaat gctttcagac
     5941 aaagacgatg ccttgttcct gcagatgctt tttatgaatg gaaacaagaa gtaaaagaaa
     6001 aaattcctta tcggatattt ctcaaaaatc aaaatatctt ttcgatggcc ggtatttggg
     6061 aaaaatgtaa gctatcaaat ggtgaaacaa ttttttcatt tgccattatc actacccaac
     6121 caaatacatt aatgactaag attcataacc gtatgccagt tattttagat aaaaaaggtg
     6181 aagatctatg gattaataac accgacgaaa aagaacttac aaatctgcta aagccctttc
     6241 cggcagaaca aatgacggca tatcgtattt cgaatcttgt aaactcacct cgtaataatt
     6301 caccaaaaat aatcgaacca attgctgata acagcctttt caattgatac tttcttcctt
     6361 tgatttaaaa tctgtcagac tattttaaac cttacttttg tttcaaattt agctttaaaa
     6421 atggatgatt tattcgctga agtattacta ccacttccac tttctgggag ttttacttat
     6481 aaaataccgt caggtttaga tgttgaagtt gaacctggga agagagttgt tgttcagttc
     6541 ggaaaatcaa agttatatac tggtcttgtt aaaacattac accacaacaa gcctgaaaat
     6601 tatactgcaa aacccattat ttcagttctt gatcatacgc cggttgtaaa tccaatacag
     6661 tttcattttt gggattggat ggcagattat tatatgtgct tcgaaggcga agtaatgaat
     6721 gccgcattgc catcggcatt aaaacttgca agtgaaacac gtatagtgca aaatccctcg
     6781 ttcaatggcg acacctctca gcttaacaat accgaatatt tagtggctga agctattgat
     6841 atacaaaaat cgctaactat ttctgaggtc tcccgcattg tggagtttaa aaaggtgatc
     6901 cctttgataa aaaatttgat tgagaaaaat gtagtgttac tggaggagga gttaaaagag
     6961 gtttatcgcc cgaagatgga aacctggatg cgacttaccg aaaaatacag aaccgaagag
     7021 gccattaaaa cattgatgga ttcactcgaa aaaagagcct acaaacagtt ggaggtaatg
     7081 atttcgtttt taagcaatat agatgacttt tcggatgaaa atgcgatgat taaaaaatcc
     7141 gacttgcttc aacttgcctc tgctgacaca caacagataa ctgctcttga aagaaagggg
     7201 gtttttgaaa taatcgaaaa agctggtagc cgtcttgagt ccttcgaggt tacaacaccg
     7261 gtctcttcta taatattaac agaaccacag caaacggcgt tcagccatat aaaacagggt
     7321 tttgaaaatc acaatgttac tttactacat ggtgtaacat caagcggaaa aactgaaata
     7381 tatatccggc taattgatga agtgatcaat agcgggaaac aggcattatt cttattaccc
     7441 gaaattgcat taaccacaca gttgataaat cgcgttagga aatattttgg aaatcaagtt
     7501 ggtgtctatc actccagata tagcatgatg gagcgagttg aaatctggaa caaagtgata
     7561 caagaatatg atagcttaac cgatgcaact gataagtaca gcgttattct tggcgccaga
     7621 tcggcattgt ttttaccttt taataatctt gggcttatca ttgtagatga agaacacgac
     7681 ggttcctaca aacaattttc tcctgcgccg cgctactcgg cacgagactc ggcaatagtt
     7741 ctggcacaaa tgcacaatgc ccgcgttttg cttggatcgg caactccatc tattgagagt
     7801 tactttaatg caaaatcggg caaatatgcg cttgctacac ttaacgaaag gtatggcgga
     7861 atggagttac ccgaaattat aatctcggat ctaaaaatcg aaacacgaag aaagacaatg
     7921 aaatcgcatt tcagttcatt gttaataaat ctaattgaac aagcgttgga aaacaaggaa
     7981 cagatcatac ttttccaaaa tcgccgtgga tactcgcttc gtatcgaatg tggtatgtgc
     8041 caatggacgc cttgttgtaa aaattgcgat gtaaccctga tttaccataa gcaaaataac
     8101 cagctcaaat gtcattattg cggatattcg atccccattc ctgcagtatg tcctgaatgt
     8161 ggttataccg ggctacacat gaaagggttt ggcaccgaaa aaattgaaga cgaactggct
     8221 ttacttttcc caaaagcctc tattacgcga atggacctcg acacaacccg aacaaaatca
     8281 tcctaccaaa atatcatcag taatttcgaa cagcggaaga ttgatatatt agtaggcacg
     8341 caaatggtta ccaagggcct cgatttcgat aatgtgagtg ttgttggaat cctcaatgcc
     8401 gacaatctgc ttagctaccc tgacttccga tcgtttgagc ggagtttcca attaatggca
     8461 caagttagcg gcagagcagg ccggaaattc aaacgcggga aagtaatcat acagagttac
     8521 cgccccgacc atgaagtaat aaaatatgtg gtgtacaacc gctacgacct tctttatcaa
     8581 aaccagatcg cagaacgacg acgatttaaa tatccgccaa tttttaggct tatcgaacta
     8641 cagctacagc ataaacaaga acaaaaggtt agtgaagctg cctatgagct tgcctcatta
     8701 ttaaagcaaa aactcggtaa cagagttata gggcctgaat tcccaattgt gtcaaaaatt
     8761 aaaggccttt atttgaaaaa catattgata aaattagaaa aaactaaaca tacaagccaa
     8821 cttaaagagg atgtaaaaga gttattagat tattttacta aaaacgccaa acacaaatct
     8881 gtcagggtaa ttgtagatgt tgatccatat taattacgtt ctaaaaaaaa gttttattaa
     8941 acaacttttt atatttgcta agaccaaaac cattcaatgt cagaatattt taagggccat
     9001 aaagtctttt cgttgcttga gatcacacaa agcatccaac gagcatttca gaagtggtat
     9061 tcagatacct actggataaa ggccgagatg aacaagctaa attattataa atattcaggt
     9121 cattgttatc ccgatctact ggaacgtaag caggaaaaga tagtagctca aatcagagcg
     9181 accctttggg atattgacta ccaacgaatt aactctacct ttctgaaagt gctcaacgag
     9241 cctttgaaag acggaattaa tattcttttc ctggcaaaga tatcattcac tcctttacat
     9301 ggtcttagcc tctctatact cgatattgat ccctcctaca ctcttggcga tttagaacgt
     9361 gaaaagctca acaccattaa ccggttaaaa agttccaata ttttcgacca aaataaacgt
     9421 ttacaagcca gccctctcat tcagcgcctt gccatcatct ctgtcgaaac cagtaaagga
     9481 ttatctgatt ttatgaaaat catcaacgat aaccgctggg gttatagatt tttcacaatg
     9541 ctctttcctg cacttttgca gggcgataaa gctcccgagt ccattatggg acagttgcga
     9601 aggataaaca gagtaaaaca tcacttcgac gctgtggcaa tcatcagagg tggaggtggc
     9661 gatattggac tgacaagtta caataatttt caactggcac atgccgtagc tacttttcct
     9721 ttaccagtac taaccggtat cgggcattct acaaatgaaa cagtaacaga gatggtggca
     9781 cacatcaacg caattacccc taccgagctg gcagattttc ttattcaaag gtacaggaac
     9841 ttcgacatac cggtaaccga agctgctaag aggatcaaat tactttcgca acaaaaaatc
     9901 agctccgccg aacaaaaatt gaaaaatgaa atgcggtttt ttaaatcggt agccgaaaaa
     9961 aagataacaa cgcaaagcga ggttattctt agcaaaatcc aacagatcag gcaaaacata
    10021 cgttcggagt ataaagtaca gctttcaaaa ttagaagcct taaagctcaa tttaaataaa
    10081 catacaccat cggcctataa aaacgaattt gataagatcc ggaatttaaa actgaatctg
    10141 aaaaaaaact ccgggatatt gttaaaaaat ttcagtaatg aaattctatc aatagaaaat
    10201 caacttcgag cgctccatcc tgataatgtt ctaaaaagag gattcagcat aacgttacta
    10261 aatggaaaag cgctgacaaa agccaataat gcatctgttg gcgatgaagt tgagactatt
    10321 ttattcaaag gagcaatcaa aagtaaaata acacataaat caattaataa agaccatggc
    10381 taaacaacta acatacatcg aagcctttga ggaacttcag cagatcgtca acgatatcga
    10441 agagggggaa acaggaatag atcagctttc ggaaaaagtt aaacgagccg ccgagttgat
    10501 caaaatttgt aaaaacaaac tgatttccac tgaaagcgac gtcaacaaaa tccttgatga
    10561 gttggaagaa acctcaacaa atagtgttga agccgaaacc tgatagacct ataatgattt
    10621 tggcaataat aaaagtttaa cacagattag caattcatgg tatgaattac ctctccgttt
    10681 aaagtcaata tttcttttat cattatagtg gtaaggcaag aatgtacagg caaaatgcca
    10741 ataaataccc cagggctgaa tttgtcaata tgttcagggg tagtttttat tatcccatgc
    10801 tcttgcgaaa cggattttac aaaagttcca tctaatggta acgtccagcc aaatttatca
    10861 agtagaacaa cctttccaaa aacctttcca ccaccttcat catttaatgc ctcttttgag
    10921 agatgagcag ctccgcccca aataacaact tctaaacgct caacatgtac tgcgactatc
    10981 gggcaggcaa gcgcaacagc tatctggtcg tatcggcaaa cgccgagttc ctgttgtatc
    11041 aaatcataaa aaacaaaatt gccaggacga atttcatcaa catcttcaaa attccggcaa
    11101 agggaaactg caggtgtgtc gccaatagag atttcagggt ta
//
LOCUS       DAOOAI010000077        11087 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_7492_length_11087_cov_4.662618, whole genome shotgun sequence.
ACCESSION   DAOOAI010000077 DAOOAI010000000
VERSION     DAOOAI010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 11087)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11087)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11087
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7492_length_11087_cov_4.662618"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1262)
                     /locus_tag="PK345_06455"
     CDS             complement(<1..1262)
                     /locus_tag="PK345_06455"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140658 - ATP-dependent chromatin
                     remodeler activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2-related protein"
                     /protein_id="HPX34690.1"
                     /translation="MIDLKMSQQVIENIRNLLSLELSRSGSGTMIYKQGHNLYLNGQC
                     SLLSESEQHFRFLIDDKYGDFIVEVSLEKEPQLQCTCQSATLCRHQTAALLQLEELIR
                     LKEDKIIHEGIKYTRKGMIQRVIEERKKKAEQARYSIEFSDNIFGEHVLTNERGVQYK
                     LTFRDINRKHGYCNCPDYRINRLGTCKHLIYAFTQLQNDPDRIPQTPIPYPFIEVFLN
                     PFRNNKISYFFPEKITGQIAELFYRYFGNRNFIEEDDIERFTGFLNNTHRFKKILVRP
                     EVYEKVKKVTKQLAISRVKENKKFNLNLLKTNLLSFQVDGVEFATFNIGAMLADDIEL
                     GRITQAVATAIMKKDLFGFSRTLIICPANLRHHWKREIENLSNERLCIIERQPEDQAG
                     IYQNAKDFFILINYEKINQYLEVIKESPP"
     gene            complement(1294..3597)
                     /locus_tag="PK345_06460"
     CDS             complement(1294..3597)
                     /locus_tag="PK345_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006744296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HPX34691.1"
                     /translation="MPDSFLKNLNEAQRVAVMHTEGPSMVIAGAGSGKTRVLTYRVAY
                     LLQRGVDPFQVLALTFTNKAALEMKERIVALIKTSDAHNVWMGTFHSIFARILRIDGH
                     LLGYPSNYTIYDTDDSKSLLRTIVKEYNLDPKVYQPGWLLARISAAKSSLVSDYEYNQ
                     NPELTESDNRSGRPLTGKIFTAYNIRCRKAFAMDFDDLLFNMNLLLRDFPEILLKYQS
                     KFRYILVDEYQDTNFAQYMIVKKLAARHENICVVGDDAQSIYSFRGANIQNILNFKND
                     YPDLKIFKLEQNYRSTKMIVEAANSIIINNKDQIFKKIWTHNEEGNRIKLMRAKTDNE
                     EGNIVASSIFEIRMSNQLPNKDFAILYRTNAQSRSFEEALRKLNIPYRIYGGLSFYKR
                     KEIKDLLAYFRLVVNPLDEEAFKRIINYPARGIGETTIDRLLVAAQRLDKPVWHIIEN
                     IFDLDLNINQGIKNRISDFAMMIKRYAADLKKRNAYELAKDIGRASGIFKELDEDKTP
                     EGISRIENIEELFNAIKEFSESDDLTSLEEGNNLRTLDIYLQDISLLTDADHDDKGDS
                     NKVSLMTIHSAKGLEFPYVYIVGLEENLFPSIQSIGSRNDLEEERRLFYVALTRAEKA
                     AFISYAETRYRWGNLTMSEPSRFIYEIDPSYIEQPKKVVNHPSDLNKFRYTGEELPKK
                     KPVSFNKDRFKKIEGAQYSTSDSEPFESAEIDRIHAGMQVEHQRFGKGKVLSTEGIGA
                     NRKATVFFPAVGQKNLILKFARLKILE"
     gene            3829..5271
                     /locus_tag="PK345_06465"
     CDS             3829..5271
                     /locus_tag="PK345_06465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010802284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPX34692.1"
                     /translation="MFASQFNLFNLVNSFLVDPVFRTLSDIASNEGVRAWVIGGYVRD
                     LFMHNPSKDIDIVVEGSGIGFAQAVARYTNACNLQVFKNFGTAMLSYKDWKIEFVGAR
                     KESYRLSSRKPIVENGTIEDDQKRRDFTINAMSLSLNKADFGQLIDPFGGIRDLDLKI
                     IRTPLDPDITYSDDPLRMMRAIRFASQLNFKIEEKSLKAITINSERIKIVSQERITDE
                     LNKIIMAAKPSVGFKLLNETGLLKIIFPEMVELQGAEYKEGRGHKDNFLHTLIVLDNI
                     ALKTENLWLRWAAILHDIGKPATKKWVDGIGWTFHGHDFVGSKMIPSIFRRMKLPKGD
                     HLKYVQKLVALHLRPIALVEDVVSDSAVRRLLFEAGNDIDDLMTLCSADMSSKIPEKI
                     QRYRENFDLVRTKLIEIEEKDRLRNWQPPITGDIIMTTFNIPPCKEVGIIKTAIREAI
                     LEGNIPNEFEPAYQLMLQTGKELGLKQVSN"
     gene            5342..5917
                     /locus_tag="PK345_06470"
     CDS             5342..5917
                     /locus_tag="PK345_06470"
                     /inference="COORDINATES: protein motif:HMM:NF019542.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34693.1"
                     /translation="MKVFKFRIETDEDPTFILDLEIRSDQTFKDLHDVLVKTLKFTEN
                     ELASFYVADENWEKYEEITLLDMIGEEEEELYDDEKDLKNIFLMSSTPIGKFITETGQ
                     NLVYEYDFLQLHTFFIECTEVKEIDKKGSYPKIVGQKGGYKVINKIHIEDDPDKLKAS
                     LLQDFNSMVNSNLDDDDDDNDDMELDGNDDF"
     gene            5935..6870
                     /gene="miaA"
                     /locus_tag="PK345_06475"
     CDS             5935..6870
                     /gene="miaA"
                     /locus_tag="PK345_06475"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008861746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HPX34694.1"
                     /translation="MDFRKGSEKSNLLVVITGPTAVGKTRLAAKLAQHYRTVVISADS
                     RQFYRELRIGTAFPTPEELDLAPHHFVGHLSVNDDYNVSKYENEVLKFLDQAFKKHHL
                     IILTGGSGLYVDVVCKGIDDLPEADPDIRRQIDKWLNEKGIEFLQEKLKAVDPKYFAT
                     VDKNNPKKLARAIEVFLLTGIPYSLLRKNQPKKRPFHILKIGLNMQREELFERISLRT
                     DKMIEDGLVEEVRSLADYRNLNALNTVGYKEIFEYLDGKLSFEQAVINIKTNTRRYAK
                     RQLTWLKRDKEIKWFEPDQLMLIINYIDEFNSSSK"
     gene            complement(7059..7901)
                     /locus_tag="PK345_06480"
     CDS             complement(7059..7901)
                     /locus_tag="PK345_06480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HPX34695.1"
                     /translation="MIIVVESGSTKTDWRIIKSDGIQIKYCTEGFNPNYNPESILSSM
                     VEYISMNGDTDGAVSLFFYGSGCSSEISIKKVTSAIKKILPTINIEIQHDLLGAARAL
                     YGNGTGIASILGTGASSCLFVNGKILEAVPSLGFLLGDEGSGLHLGRLLLNAFFKKNL
                     PPHLYEAFQKKYKLVLADFISHLYSHPKPNSFIASFVPFMVENKDESAIKELVEAAFD
                     EFINQIILKYSDCHNYELGFVGSVAYLFSDLLTKKANEYGLTIGKIIQTPIEGLVEFH
                     SKGI"
     gene            8062..>11087
                     /locus_tag="PK345_06485"
     CDS             8062..>11087
                     /locus_tag="PK345_06485"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPX34696.1"
                     /translation="MKNVINQYINNSVLLFLTFTTVFVLFVQQRALSQDFAINFGRSV
                     SDIVVHEDNMQNFRLTFTYSGLGTFIVETDSDMSFNEIAIPGTYSIGEIGTPKLPASK
                     KLMEVPFGAEIEVKVKNYTTTEYQLSDYGINFPIIPVQPSLRKDQNVSDVPFEFDKNI
                     YEKDFFIKHELASVEVLGVLRGYRIARLTIAPVSYNPYKGIIKVYNDIEVEISFINAD
                     EALNDYIKATTWSPYFEVVGKRLINNIRNGGYPAHPDLTKYPVKYLIVSNRMFENDLQ
                     PFIEWKIKKGYKVIVAYTDEIGNTYTQIQSYIHQQYNQGTPTDPPPSFVLLVGDTPQL
                     PSTSGSSTGKMTDLYYASVDGDYFPEMYYGRFSATNSAQLVSQIAKTLYYERYEFEDP
                     TYLNDVTLIAGADGTWNPRIGQPTVQYGTINYFNAAHGYANVNAYLTSPYTGCYSPEK
                     IAVSMINYSAHCNETCWGDPNLTISMVNAFVNNGKYPLAVGNCCLAADFGYGECIGET
                     WQRAANKGSVAYIGSAPSSYWFEDFYYSVGAFPLQGNNNGYVPTFEETTWGAYDAPFV
                     SDYVTTGSLNFVGNLAVTEVHIQGWPSHSSPLYYWQAYNVLGDPSLVAYHTEGTENTV
                     SHMPIVPIGMNYYEVTAEPGSYVGISKDGVLHGCALVDETGIVEVPMDPILDGGMVDI
                     VVTKPQFIPYMVQIPAAALEGPYIVLDGYTISDPTGNNNGLADYGENINLNVTLKNVG
                     ADPSASVNATVSGSDQYVTLTSTATKNFGTIPAGGTATVNNAWSFSIDDFVPDQHKAQ
                     FILTMTDGSDTWTSNLFITIQSPIIFIAEEFLVDDSQSGNNDGILDPGETALIIMNVK
                     NNGHSAISNTIINITSTDPMLTINTPTVNIASLDAGASQEYSFNVSASETSPIGYPVN
                     VNLNAEGGPDGQYTASCLITVVIGLIPEYIMTNATITTCVGEFYDSGGPNGQYGSNED
                     LVMTFLPLTDDGIIKAEFISFETETNYDFLYIHNGA"
BASE COUNT         3310 a         2115 c         2198 g         3464 t
ORIGIN      
        1 ggaggagatt ctttaataac ttcgagatat tgatttatct tttcgtaatt tataaggata
       61 aaaaaatctt tagcgttttg ataaatgcca gcttgatctt ccggctgcct ttcaattata
      121 cataacctct cgttcgacag attttctatt tctcttttcc aatgatgcct aaggtttgcc
      181 gggcaaataa ttaaagtgcg tgaaaacccg aacaaatctt ttttcattat tgccgttgcc
      241 accgcctgag ttattcgtcc cagttcaata tcatcagcca acatggctcc tatattgaaa
      301 gttgcaaatt caactccatc aacctgaaag gacagaaggt tggtttttag cagattaagg
      361 ttaaactttt tattctcttt tactcgactt atagctaatt gcttggtaac cttttttact
      421 ttttcataga cttcgggccg cacaagaatt tttttaaacc ggtgggtatt atttagaaac
      481 cctgtaaaac gttctatatc atcctcttca ataaaatttc tatttccaaa atagcgataa
      541 aaaagctcgg ctatctgccc ggtaattttt tcagggaaaa agtaacttat tttattgttg
      601 cgaaacgggt ttaaaaagac ctcaataaag gggtagggga ttggcgtttg aggtatcctg
      661 tcgggatcgt tttgaagttg tgtaaatgca tagataagat gtttgcatgt tcctaatctg
      721 tttattcgat aatcaggaca gttgcagtat ccatgttttc gatttatatc cctgaaggtg
      781 agcttgtatt gtactcctcg ttcgttggta agtacatgct cgccaaaaat gttatcagaa
      841 aactcaatac tatatctggc ctgctcggct ttcttttttc gttcctcaat aacacgttga
      901 atcattccct ttcgggtata ttttatccct tcgtgaatta ttttgtcttc tttaaggcgt
      961 atcagctctt caagctgtaa taaggcagcc gtttgatggc ggcacaaagt tgcactttga
     1021 catgtacatt gcagctgcgg ttctttttct aaacttacct ctactataaa atccccatac
     1081 ttgtcatcta tcagaaagcg aaaatgctgc tccgactccg acaaaagcga acactgaccg
     1141 ttcagataaa ggttatgccc ctgcttgtat atcattgttc ccgagccgga gcggcttaac
     1201 tcaaggctta ataagtttct gatattttca attacttgct gggacatctt aaggtctatc
     1261 attatgttta tctaaaagca atacgaaatt taatcactct aaaattttta atctggcaaa
     1321 cttcagtatc agatttttct gcccgactgc cggaaaaaaa acagtagctt ttctattggc
     1381 tccaatgcct tcagtactta acactttccc tttcccaaat cgttgatgct ccacttgcat
     1441 accggcatgg attctatcta tttcagcgct ctcaaagggt tcgctatcag aggttgaata
     1501 ttgggcgcct tcaatttttt taaaccggtc tttgttaaat gacaccggtt tttttttcgg
     1561 caattcttct cctgtatatc taaatttatt taggtcgctt ggatggttga ctaccttttt
     1621 aggctgctct atgtacgaag ggtcaatttc gtaaataaac cggcttggct cactcatcgt
     1681 caagtttccc caacgataac gagtttcggc atatgagatg aaagcagctt tctcagccct
     1741 ggtgagagcg acataaaaca ggcgacgctc ctcttcaaga tcgttgcgcg agccgatgga
     1801 ttgaatgctg gggaaaagat tttcttcaag accaacgata taaacataag gaaattcaag
     1861 ccctttagcg ctatggatag tcattagcga tactttatta gagtcgcctt tgtcgtcatg
     1921 atcggcgtcg gtgagtagcg atatatcctg aagatagata tcgagcgttc gtaagttatt
     1981 accctcttct aatgaggtca aatcatcaga ttccgaaaac tcctttatcg cattaaacaa
     2041 ttcttcgata ttttctattc tgctaattcc ttcaggggtt ttgtcctcat caagctcctt
     2101 aaatattccg gatgctctac ctatatcttt tgcaagttcg tatgcgttac gttttttcaa
     2161 atcggcagca tatcgcttta tcatcattgc aaaatcgctg attctgtttt taattccttg
     2221 attgatgtta agatctagat caaaaatatt ttcaattata tgccacacgg gcttatccag
     2281 cctttgcgct gcaactaaca gtcggtcaat ggttgtttcg ccaattcctc ttgccggata
     2341 attaataatt cttttaaaag cctcttcgtc caaggggttt accaccaatc tgaaatatgc
     2401 taacaagtcc ttgatctctt tgcgtttgta aaatgaaagc ccgccatata tccgataggg
     2461 gatattaagt tttctcaagg cctcttcaaa tgatcgggat tgcgcattag tacggtaaag
     2521 tatggcaaaa tccttatttg gaagctggtt gctcattcgt atttcaaaga ttgagcttgc
     2581 cactatattg ccctcttcgt tatcagtttt tgcccgcatc agctttatcc ggttaccttc
     2641 ttcgttatgc gtccatatct tcttgaaaat ctgatctttg ttatttataa taatgctgtt
     2701 tgcagcctct acaatcatct ttgttgaacg gtaattctgt tcaagcttaa atattttcag
     2761 atcgggataa tcatttttaa agttcagaat attttgaata ttagctccac gaaagctata
     2821 tatactctga gcatcatctc caactacaca aatattttcg tgtcgggctg ccagcttttt
     2881 gacaatcata tactgagcaa aattcgtatc ctgatattca tccaccaaga tatagcgaaa
     2941 ttttgactga tacttcagaa ggatttcggg aaaatctctc aaaagcaggt tcatattaaa
     3001 aagcaggtca tcgaagtcca ttgcaaaagc tttgcggcat cgaatattgt aagctgtaaa
     3061 tatcttaccg gtaaggggtc ttccgctcct attgtccgat tcagtcaatt cagggttttg
     3121 attatactca tagtcggata ccagactgct ctttgctgct gaaattcttg caagtagcca
     3181 tccgggctga tatacctttg gatcaaggtt atactctttt acaatcgttc ttaaaaggct
     3241 ttttgagtcg tcggtatcat aaattgtgta gttggatggg tagccaagca gatgtccgtc
     3301 aattcttaat atccgtgcaa aaattgagtg aaacgttccc atccatacgt tatgagcatc
     3361 cgaagtcttt attagagcta ctatcctctc tttcatctca agggcagctt tattggtaaa
     3421 agtgagcgct aacacctgaa atgggtcaac gccacgttgc aacaagtaag caaccctgta
     3481 tgtcagcact cttgttttgc cagaaccggc ccctgcgata accatcgaag gaccttcggt
     3541 atgcatcaca gcaactcttt gtgcttcgtt aagatttttc agaaaactat ccggcatatc
     3601 aaaacagatt tggcttcaaa aatagtcctt tagttgcaat ccataaaata taatagggcg
     3661 atgcaagatt aaatttttga acctttaata ataagaagtt tgccttttcc ataacccttc
     3721 atgggatttt caagagtatc tgacgctgga ttttcaaaga tctgaataat ggctgcaatt
     3781 acaaaatcgc tattctcaat tttggttctc tttttttaaa aaggttttat gtttgcatca
     3841 caattcaatc ttttcaattt ggtgaattca tttcttgttg atcctgtttt tcgcacttta
     3901 tctgatattg cttcgaacga gggtgttagg gcatgggtta ttggcggtta cgttcgcgat
     3961 ctttttatgc acaatccctc caaagacatt gatattgtgg tagaaggctc gggaattgga
     4021 tttgcgcaag ccgttgccag atatacgaat gcctgcaacc ttcaggtgtt taaaaatttt
     4081 ggaacagcca tgttaagtta taaggattgg aaaattgaat ttgtgggtgc acgcaaagag
     4141 tcgtaccggt tgtcgtcgcg caaaccgata gtggaaaatg gcactattga ggatgatcaa
     4201 aagcgcagag attttactat taacgccatg agcctgagtt tgaacaaagc agattttgga
     4261 cagttaattg atccttttgg cggcatccgc gaccttgacc taaagattat tcgaacgcct
     4321 ctcgatcctg acatcaccta ttccgatgac cctctgcgta tgatgcgcgc cataaggttt
     4381 gcttcacagc ttaatttcaa gatcgaagag aaaagtctta aagcaatcac aataaattca
     4441 gagaggataa agatagtttc gcaggagaga attaccgacg aactgaataa gatcattatg
     4501 gctgctaaac cttcagtggg ttttaaactt ttgaatgaga ccggattgtt aaagataatt
     4561 tttccggaaa tggttgaact tcagggagct gaatataaag aaggtagagg ccacaaagat
     4621 aatttcctgc acacacttat tgtgttggac aatatagcgc ttaaaacaga aaatctttgg
     4681 ctcaggtggg cagccatttt gcatgatata ggaaagcctg caacaaagaa atgggtagat
     4741 ggtattggat ggacttttca tggacatgac tttgttggat cgaaaatgat accgtcaata
     4801 tttcgtagga tgaagctacc gaaaggagat catttgaaat atgtacaaaa attggttgcc
     4861 cttcatctgc gccccattgc tttggtagag gatgtggtga gcgattcggc cgtgcggaga
     4921 ttgctttttg aagcaggtaa cgatattgat gatctaatga cactctgttc tgctgatatg
     4981 tcgtcaaaaa taccggagaa gattcaacgt tacagagaaa attttgacct tgtcagaaca
     5041 aaactcattg agatcgaaga aaaagatagg ttacgtaact ggcaaccgcc aattactggc
     5101 gatatcataa tgacgacttt taacatacca ccttgtaaag aggttggcat tattaaaact
     5161 gctattcgcg aagctattct ggaaggtaac attcctaatg aatttgaacc ggcttaccag
     5221 ttgatgttgc aaacaggaaa agaattaggc ttgaaacagg tttcaaatta gatttttttt
     5281 gaaatattga ttttaattta tttttggctt tttaatctat aaattcgaac aagacaaaaa
     5341 aatgaaagtt tttaagttta gaattgaaac tgatgaagat ccgactttta tacttgattt
     5401 agagattaga tcggatcaga cgtttaaaga tttgcatgac gttctcgtaa aaacgttaaa
     5461 atttaccgaa aatgagctgg cctcttttta cgtagccgac gaaaattggg aaaaatacga
     5521 ggagatcaca ctgttagata tgattggcga ggaggaggaa gagctttatg acgatgaaaa
     5581 ggatttgaaa aacatattcc tgatgtcttc tacgccaata ggcaagttta ttaccgaaac
     5641 aggccaaaat cttgtgtatg aatatgactt ccttcaatta cacacatttt ttatagagtg
     5701 tacagaggta aaagagatag acaaaaaagg gagctatcca aagattgtcg ggcaaaaagg
     5761 gggctataaa gtaataaaca aaatccacat cgaagatgat cccgacaagt tgaaggcatc
     5821 gcttttacaa gattttaaca gcatggttaa tagtaatttg gacgatgatg acgatgataa
     5881 cgatgatatg gagctcgatg gcaatgatga cttttaacca taccttaata actgatggat
     5941 tttcgtaaag ggtcagaaaa atctaaccta ctggttgtta ttactggccc cacagcagta
     6001 ggaaaaacca ggctggctgc taagctggca cagcattatc gaacggtagt tatctcagcc
     6061 gattcaaggc agttttaccg ggaattgaga atcggaactg cttttcctac acctgaagaa
     6121 cttgatttag cgcctcatca ctttgttggg cacttgtctg taaatgatga ttataacgta
     6181 tcaaaatacg aaaatgaagt attgaagttt ttagatcaag cttttaaaaa acatcacctt
     6241 attatcctta ccggaggttc gggactctat gtggatgttg tatgcaaagg tattgatgat
     6301 cttcctgaag ccgatcctga tatcaggcga caaattgata aatggttgaa tgaaaaggga
     6361 attgaattcc ttcaggaaaa acttaaagct gtagatccga agtattttgc tactgtggat
     6421 aaaaataacc ctaaaaaatt agcacgcgct attgaggtct ttctgttaac cggaatacct
     6481 tattcgttac ttagaaaaaa tcaacctaag aaacgacctt tccatatttt aaaaatcggc
     6541 ttaaatatgc aacgcgaaga gctgtttgag cgtattagcc tgcggactga taaaatgatt
     6601 gaagatggtt tggtagagga ggtgcggtcg ttggccgatt atcgtaacct taacgctctg
     6661 aacactgttg gatacaaaga aatttttgaa taccttgatg gcaaattatc ctttgaacaa
     6721 gctgttatca atataaaaac aaatacccgg cgctatgcta aaagacaatt gacatggctt
     6781 aaaagagata aagagattaa atggttcgaa cccgaccaac ttatgttgat aatcaattat
     6841 atagacgaat ttaactcaag ttctaaatag agctttacca gcgctaacaa aaaaacgaca
     6901 ttttagtatt tagattcgat tcctcatttg ataatgtgaa tgaataaacg gttgtgatta
     6961 tcacaataat aattgtacta aaagaaacac aaaatcaggt gtggctccat taaacccata
     7021 atcgcgccta aaagggtttt atgttgataa attcgggtct agattccttt gctatggaat
     7081 tcgactaatc cttctattgg agtttgaatt attttcccga tggtgagccc gtattcgtta
     7141 gcttttttag ttaaaagatc gctgaaaaga tatgctaccg accctacaaa tcccaattcg
     7201 tagttatggc aatccgaata ttttaaaatg atttggttga tgaattcatc aaaagctgct
     7261 tcgacaagtt ctttgattgc ggattcatct ttgttttcga ccataaacgg cacaaatgaa
     7321 gcaatgaagc tgttgggctt aggatgggag taaaggtgtg aaataaagtc tgccagaact
     7381 agtttgtatt ttttctgaaa cgcttcataa agatggggtg ggaggttttt tttaaaaaaa
     7441 gcattaagta aaagccttcc taaatggaga ccgctgcctt catcgccaag caggaagcca
     7501 agagaaggta ctgcctctaa aatttttcca tttacgaaaa gacaactact tgcgccggta
     7561 cccaatatcg aagctatgcc ggtaccatta ccatatagcg ctctggcagc gccaagcaaa
     7621 tcgtgttgta tttcaatatt tatcgtaggt agaatctttt taatagctga agttactttt
     7681 ttaatcgaaa tttctgacga acacccagag ccataaaaaa ataaagatac ggccccatcg
     7741 gtatcgccat tcatactaat atactcaacc attgagctaa gtatggattc aggattatag
     7801 tttggattga aaccttcggt acaatatttt atttgaatac catcagactt tattatccgc
     7861 caatcggttt ttgtagagcc actctcgaca acaataatca taaacttttt ttaaaaaaaa
     7921 tcatgacaaa ggtaactcaa tttttaaagt ccctcgggta ttttacctga gatggtaaaa
     7981 aagtcagtta tgtaaaaaaa agtatttttg ttactcaaat aaattcattc tttttgttaa
     8041 ttaactatca aattaagacc tatgaaaaat gtaattaatc aatacattaa caatagtgtt
     8101 ttgctattct tgactttcac tactgttttt gttttgttcg ttcagcaaag agcattatct
     8161 caggattttg caataaattt tggacgttct gtatcggata tcgttgtaca cgaagacaat
     8221 atgcagaact tcaggctcac ctttacttac agtggattag gaactttcat cgttgagacc
     8281 gatagtgata tgtcatttaa tgaaattgcc attcccggca cttattcgat aggtgagatt
     8341 ggaactccta agctaccggc ttccaaaaag cttatggagg taccatttgg cgctgaaata
     8401 gaagtgaagg taaagaatta taccactacg gaatatcaat tgtcggacta tggaattaat
     8461 tttccgataa tcccggtaca gccttcgtta cgcaaagacc agaacgtgtc cgatgttcct
     8521 tttgaatttg acaaaaatat ttatgaaaaa gatttcttta taaaacatga attagcctcg
     8581 gttgaagtac ttggcgtgtt gagaggatac aggattgcac ggctgacaat agcgcctgta
     8641 agttataatc catacaaagg aatcattaag gtttataacg atattgaagt tgagataagc
     8701 tttattaatg ctgatgaagc gctcaatgat tatatcaagg caactacgtg gtcgccttat
     8761 ttcgaagtgg ttggaaagcg tttgatcaat aatatccgca atgggggata ccctgcacat
     8821 ccagatctga ccaaatatcc ggttaaatac cttattgtgt ccaacaggat gtttgaaaat
     8881 gatctacagc cttttattga gtggaagatc aagaaaggtt ataaagtgat tgtggcttat
     8941 actgatgaga tcggaaatac ctacactcaa attcagtcgt atattcatca gcagtataat
     9001 caaggaaccc cgaccgaccc gccaccaagc tttgtacttt tagttggtga tacgccacaa
     9061 cttccatcta catctggctc ctccaccggt aaaatgacag acctttatta tgccagtgtt
     9121 gatggcgact atttccctga gatgtattac ggaaggtttt cggctactaa ttcagcacaa
     9181 ttggtttcgc agattgcaaa gactttgtat tatgagcgat atgaatttga agaccctact
     9241 tatctgaatg atgttacgtt aatcgccgga gctgatggaa cgtggaatcc cagaatcggg
     9301 cagccaacag tacaatacgg cactatcaac tattttaatg ccgctcatgg atacgctaat
     9361 gtaaatgctt atttaacaag cccatacaca ggttgttatt cgccggaaaa aattgcagta
     9421 agcatgatta actactctgc ccattgcaac gaaacttgtt ggggcgatcc taacctcact
     9481 ataagtatgg taaatgcctt tgtcaataat ggaaagtatc cattagctgt tggtaactgt
     9541 tgccttgctg ccgattttgg ttatggcgaa tgtattggag agacttggca acgtgctgcc
     9601 aacaaaggtt cggttgccta tatcggctcc gcaccaagta gctattggtt cgaggatttt
     9661 tactattcag tgggagcttt tccattgcaa ggaaataata atggttatgt gcctactttc
     9721 gaggagacca cctggggcgc ttatgatgct ccgtttgtga gcgattatgt aaccaccgga
     9781 tctctaaact ttgttggtaa tcttgctgtt accgaggtgc acattcaggg ttggccaagc
     9841 cactcaagcc ctctatacta ttggcaggct tataatgtgt tgggcgatcc ttcgttggtg
     9901 gcatatcata cggaaggtac cgagaacaca gtgtcgcaca tgcctattgt gcctattggg
     9961 atgaactatt atgaagttac tgccgagcca ggatcgtatg tcggaatatc gaaggatggc
    10021 gtactgcacg gatgcgccct tgtagatgag acaggtatcg tagaagtacc tatggatccc
    10081 attcttgatg gtggtatggt ggatattgtt gttacaaaac cacaattcat cccttatatg
    10141 gttcaaattc cggcagcagc tctggaaggt ccgtatatag tccttgatgg ctatacaatc
    10201 agcgatccaa caggtaacaa taatggtttg gcagactacg gtgagaatat taaccttaat
    10261 gttactttaa aaaatgtcgg agcagatccg tccgcctctg tgaatgctac cgtttctggt
    10321 tctgatcaat atgttacact taccagtact gctaccaaaa attttggaac tataccggca
    10381 ggaggaactg ctaccgttaa taatgcttgg tcattttcaa ttgacgattt tgtacccgat
    10441 cagcataagg ctcagtttat acttacaatg accgatggaa gcgatacttg gacttccaac
    10501 ctgttcatca ctattcagtc gccgataatt tttattgccg aagaatttct tgttgatgat
    10561 tcacagtcag ggaataatga tggaatcctt gatccaggtg agacagctct tataataatg
    10621 aatgtcaaaa ataacgggca tagcgcaata tcaaatacca ttatcaatat tacttctacc
    10681 gatcctatgt tgacaatcaa tacccccact gttaacatag caagccttga tgcaggcgcg
    10741 tcacaggaat attcattcaa tgtctctgcc agcgaaactt caccaatagg ttaccctgtc
    10801 aatgtaaacc ttaatgctga aggaggacct gacggacaat atacagcaag ctgtttgatc
    10861 accgtagtaa ttggtttaat acctgaatac ataatgacta atgcaaccat aacaacatgc
    10921 gtcggtgagt tttatgactc aggtggtcca aacggacaat atggaagcaa tgaagacttg
    10981 gttatgacct ttctgcctct tactgatgat ggcatcatca aagctgaatt tatctctttt
    11041 gaaacagaga ccaactacga tttcttatac atacataacg gcgccaa
//
LOCUS       DAOOAI010000078        10501 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_7970_length_10501_cov_5.168677, whole genome shotgun sequence.
ACCESSION   DAOOAI010000078 DAOOAI010000000
VERSION     DAOOAI010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10501)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10501)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10501
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7970_length_10501_cov_5.168677"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /gene="rnpB"
                     /locus_tag="PK345_06490"
     ncRNA           <1..270
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="PK345_06490"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            381..827
                     /gene="smpB"
                     /locus_tag="PK345_06495"
     CDS             381..827
                     /gene="smpB"
                     /locus_tag="PK345_06495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HPX34697.1"
                     /translation="MANIIEIKNKKASYQYFLIEEYVAGIQLTGTEIKSIRDGKANIA
                     DAYCLFIDNELYVREMHIAIYTLGTIYNHEPKRDRKLLLTRRELKKLQGKVTERGFTI
                     IPTLLFVNEKGLAKLSIALAKGKHFYDKRETLKKKDVSRYIEKGDY"
     gene            941..1486
                     /locus_tag="PK345_06500"
     CDS             941..1486
                     /locus_tag="PK345_06500"
                     /inference="COORDINATES: protein motif:HMM:NF024498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin fold domain-containing protein"
                     /protein_id="HPX34698.1"
                     /translation="MKNLFFSFIFIMILTVAEGQETPKIKWYSIEEAVELNSKSKAKD
                     KKKFFIDVYTDWCGWCKKMDANTFTDPVIVKYMNEKFWPVKFNAESSEPIKINEQTYI
                     NPNPTAKRSTHQLAIALLNKRLSYPSFVFLDQEVKLITVLPGYNTPEKLEPVLHFIAR
                     EAYKDQTFDQFKSTFKGSFSN"
     gene            complement(1529..2689)
                     /locus_tag="PK345_06505"
     CDS             complement(1529..2689)
                     /locus_tag="PK345_06505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HPX34699.1"
                     /translation="MTKILVVDDERSIRNTLRDILEYEGYKVDDAENGIEGLKLVNSK
                     KYDLILLDIKMAGMDGIETLEHIMKVSDTPVVMISGHGTIETAVEAIKKGAYDYIAKP
                     LDLNRLLVTIRNAVDKSNLIKETKTLRQNVPQTWEMIGNSPAMIQIKELIDKVAPSNA
                     RVLIHGSNGTGKELVARWLHEKSQRAQAPFIEVNCAAIPSELIESQLFGHEKGSFTSA
                     IRQRKGDFELAHGGTLFLDEIGDMSHSAQAKVLRALQENKITRVGGEKDIQVDVRILA
                     ATNKNLKEEIDNGNFREDLYHRLSVIIIEVPDLNKRDGDIPLLADHFMDDICKKMGKP
                     ILTFSKEAYKKLEEYQWTGNVRELRNVVERLAILCDKQVTGDDIDKYLQPLK"
     gene            2941..4041
                     /locus_tag="PK345_06510"
     CDS             2941..4041
                     /locus_tag="PK345_06510"
                     /inference="COORDINATES: protein motif:HMM:NF021179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2027 domain-containing protein"
                     /protein_id="HPX34700.1"
                     /translation="MNFKVGDKVKFLNESGGGEIKKIISSNLVSVQIDDGFEIPTLIT
                     ELIKVDEEPETPKMMFTQQQFRRQPPAPTETSDDDENYYEEGRVSPLEIFRSRGDDKK
                     GIYLAFVPHDQKWLLTGLLDIYLVNYSEYEALFSLFLRKPNGAWEGVDYDVIKQNSKI
                     LLDTIKREEIEKWSYGIFQILFHKDLSSKVLSPLSSVFSFKPTKLYHENAYQNSVFIS
                     GKAFLLIVCELALHPVVAVSELNEKFDEEVLQQTAKMKKPEEFINKHKTSPREAVVDL
                     HIEELLDNYSKMTNHEILNYQLNYFTKCLESGVNNYLTKITFIHGIGDGVLKARMLEI
                     LKEYDNVQTKDASMHQFGYGALDVLIWHSAQV"
     gene            complement(4086..4847)
                     /gene="rsmA"
                     /locus_tag="PK345_06515"
     CDS             complement(4086..4847)
                     /gene="rsmA"
                     /locus_tag="PK345_06515"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HPX34701.1"
                     /translation="MQSVVPKKSLGQHFLKDENIARKIVSSLKANERILEIGPGTGVL
                     TKYLIPVCKDFKAIEIDKEVVGFLEKEFPELENRIINDDFLKCDLNSLFEGSFSIIGN
                     FPYNISSQIFFKIINYQAIIQEIVCMLQKDVAERLAAKPGNKSYGVLTVILGAYYKIK
                     YLFTVNEQVFFPPPKVKSAVIRLERTAEKYPDIDEKLFFNIVKTGFNQRRKTLKNSLR
                     KLWTDKINPDDYPVFNKRPEQLTINDFFDLTKILS"
     gene            complement(4928..6829)
                     /locus_tag="PK345_06520"
     CDS             complement(4928..6829)
                     /locus_tag="PK345_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34702.1"
                     /translation="MVCIISMSVNAMKSYFYIPFILLFLGYAPLYGQDIPAFPKAETA
                     INKKQEPDKEKLAAEYVRNRDFEKAVLLYEQLFNEKGTHFYYNYYIYCLIELQDYKKA
                     LKAITKQQKSNPNNLRYDVDEGYIYLISGELVKANKIFDKAFENIGSTANQIRDLANA
                     FNYRGQSDWAIKTYLKGKILVPDHPFNLDLAFIYQRQQNYEMMFNEYLDLLDIDQSKI
                     ETVKSRLQSALSDDTEGEKNEMLRKELLRRIQRAPDKTYFSEMLIWHSVQQHDFEMAF
                     IQARSLDLRFQEQGQRIYELANLCMSNESYDVAINAYSYLLEKGTSSPFYLDSRIGLL
                     NARYLKVTTRYDYTHKDLIELKNEYQKALDEFGQNTSTVSIMRYMAHLQAFYLDDIDQ
                     AISLLQKAVEMPSVAKNMKAECKIELADILLFSGNVWDATLLYSQVEYDFKHDPIGHR
                     AKLQNAKLSYYIGEFGWAKAQLDVLKAATSKLIANDAMELSLLISDNLDLDSTYTALG
                     IFARAELLFYRNKYDEAWATLDSIQMLSLWHSLNDDVLFKRAEIMMKKGKYNEADNLL
                     DKLVTMYPYDLLADDALWLRADMMENHFKDIVRAKELYEKILFDYPGSLYTIEARKRF
                     RILRGDMTN"
     gene            complement(6890..8161)
                     /gene="serS"
                     /locus_tag="PK345_06525"
     CDS             complement(6890..8161)
                     /gene="serS"
                     /locus_tag="PK345_06525"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HPX34703.1"
                     /translation="MLQIHILRQNPDFVIERLKVKNFDASELITNILALDEKKRENQV
                     KLDATLSEANKIAKTIGEIIKSGKQDQATQLKERSVTLKAEAKTMANDLELLNNEINS
                     LLLQIPNIPGDQVPQGKTAEDNEIVFSFGKIPTLSPSALPHWDLIKKYDIIDFELGNK
                     VTGAGFPFYRGKGAKLQRALINFFLDEGLKAGYTEVQPPLLVNEASAYGTGQLPDKDG
                     QMYNVGLDKFYLIPTAEVPLTNIYRDTILKESDLPIKNIAYSNCFRREAGSWGADVRG
                     LNRLHQFDKVEIVQIATPETSYQVLEEMREYVSTLLVKLELPFRIVKLCGGDISFTSA
                     MTYDFEVYSAAQKRWLEVSSVSNFESFQANRMKLRFKNKNGKTELAHTLNGSALALPR
                     IVAALLENNQNETGIKISDSLIPYTGFKMID"
     gene            complement(8253..8528)
                     /gene="rpmA"
                     /locus_tag="PK345_06530"
     CDS             complement(8253..8528)
                     /gene="rpmA"
                     /locus_tag="PK345_06530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HPX34704.1"
                     /translation="MAHKKGAGSSSNGRESHSKRLGVKIYGGQFAKAGNILVRQRGTV
                     HNPGVNVGMGKDHTLFATVDGTVEFKRRKNNKSFVSVIPFEDQNTDN"
     gene            complement(8906..9220)
                     /gene="rplU"
                     /locus_tag="PK345_06535"
     CDS             complement(8906..9220)
                     /gene="rplU"
                     /locus_tag="PK345_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HPX34705.1"
                     /translation="MYAIVDIAGQQFKIEKGKQIYVHRLEGLEGDTVTFDKVLLLDNN
                     GNISIGQPFVDGASVSATIVSHLKGDKVIVFKKKRRKGFQKETGHRQYLSRIRIDEIT
                     VG"
     gene            9635..>10501
                     /locus_tag="PK345_06540"
     CDS             9635..>10501
                     /locus_tag="PK345_06540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34706.1"
                     /translation="MKQITCFLLVFFFMISSTVLFSQQPTSLDKLKMEQMEKYVNSDN
                     GPSVKATAQPVEGEHYTSYATYYPSRSELYNNGPFITHPGGGAGGKDASAVQTDLGMS
                     AYGPNVNHNMSPGNYYYLADDFEVVGTWNLESIKFYGYQTGSGTTSTFTGIYVQIWDG
                     APNAGGAVVWGDLTTNRMLSTQFSDVYRVLDTGLTNTDRPIMEIIADVSGCTLNSGTY
                     WVQWGLTGSAASGPWGVAITILGQTTTGNGLQAISTGWQNWQDSGTSTGQGGVFIIEG
                     TAGSQPTKDVGVQ"
BASE COUNT         3071 a         1904 c         2034 g         3492 t
ORIGIN      
        1 cgaattttcg gcaggtaagg gtgaaaatgt gaggtaagag ctcacacatc atgctggcga
       61 cagtgtgttg gggtaaacct tatgggttga aaggctatgt atatcacggc cccggtttac
      121 cgggataatg gctgctcgtc gaattgctgt tggcgcagca agccgtgagg ggtaagccga
      181 ttgagtctgg ctgtgaagcc agtccaagat aaatgacaga actactcgta atagagttga
      241 cagaatcccg cttacagggc tgcagctttt tttttattta aaattaaaga gcaaaagttt
      301 tttttaaaat gggggtattt cgtgtttttc aaaaaaaaga atgacaatta tccagcttaa
      361 ccattatttt tgctgtcgct atggcaaata ttatagagat aaaaaataaa aaagcttctt
      421 atcaatattt cctgattgaa gagtatgttg ccggtattca acttaccggt actgagataa
      481 aatccataag ggatggaaaa gccaatattg ctgatgctta ttgtttattt atagataatg
      541 aattgtatgt gagagagatg catattgcaa tttatacttt gggtactatc tataatcatg
      601 agcctaaacg tgacaggaaa ttgttattaa cccggcgcga gttgaaaaaa ttgcaaggga
      661 aagtaactga aaggggcttt acaattattc ctacattgtt gtttgtcaat gaaaagggtt
      721 tagcaaagct ttctattgcc ctggcaaaag ggaagcattt ttatgataaa cgtgaaacat
      781 taaaaaagaa agatgtttca agatacattg aaaaaggcga ttattaaaac caaagaaatc
      841 aatagaaagc cctggtatgc tgattgtaat cacttaatct tgtaataaac atatttaatg
      901 tgagatttgt taataatcat aatttaaatt gatcctaacg atgaagaacc tatttttttc
      961 tttcattttt ataatgatcc ttactgttgc cgaaggtcag gaaaccccca aaatcaaatg
     1021 gtattccatc gaagaggccg ttgagttgaa cagtaagtcg aaagcaaaag acaagaagaa
     1081 gttttttata gacgtctata ccgattggtg tggttggtgc aaaaaaatgg atgccaatac
     1141 ttttactgat cctgttattg taaaatatat gaatgaaaaa ttctggccgg taaaattcaa
     1201 tgctgagtcg tcagaaccta ttaaaatcaa cgagcagaca tacattaatc ccaatccaac
     1261 tgcaaaacgc tctactcatc agcttgctat cgcactatta aataaaagac tctcttaccc
     1321 ctcttttgtt tttttggatc aggaggtaaa gctcatcact gttttgccag gatataatac
     1381 ccctgaaaag cttgagccgg tgttgcattt tattgcacgg gaagcttata aagatcagac
     1441 ttttgatcag tttaaatcta cctttaaagg gagttttagt aactaataaa gggctgtttg
     1501 attacaaaac cttctatact gatccttttt attttagcgg ttgaaggtat ttgtcaatat
     1561 cgtctcctgt aacttgtttg tcgcaaagga tagctaatct ctccaccaca tttcttaact
     1621 cgcggacgtt gcctgtccac tggtactctt caagtttttt atatgcctct ttggaaaatg
     1681 taaggatagg cttgcccatc tttttgcaaa tgtcgtccat gaaatgatca gccagcagtg
     1741 gaatgtcgcc atctctttta ttaaggtcag gaacttcgat aatgataacg ctcaagcgat
     1801 ggtataggtc ttctctgaaa ttcccattat caatttcctc tttaagattt ttattggtag
     1861 ctgctaatat tcgtacatca acctggatat ctttttcacc tcctactcgt gtgattttat
     1921 tttcctgtaa tgctctcaat actttggctt gcgccgaatg gctcatatct cctatttcgt
     1981 ctaagaatag tgtgccgcca tgcgctaatt cgaaatcgcc tttgcgttgt cggatagctg
     2041 aagtaaaaga gcctttttcg tgtccaaaca attgactttc aattagctcg gaagggattg
     2101 cagcgcagtt gacttctata aacggggctt gagcacgctg gcttttttca tgaagccatc
     2161 tcgctaccag ctctttgccc gtgccattgc tgccatgaat taaaacacgt gcattggatg
     2221 gagcaacctt atcaattaac tctttgattt gaatcattgc cggcgagtta cctatcatct
     2281 cccatgtttg cggaacattt tgtcggagag ttttggtctc ttttatgagg tttgacttat
     2341 caacggcatt cctgatagtt acgagcagcc tgtttaggtc caatggtttg gcaatataat
     2401 cgtaagctcc ttttttaatg gcctctacag cagtctcaat agtgccgtgt cccgaaatca
     2461 taaccacagg cgtgtcggat actttcatta tgtgttctaa ggtctcgata ccatccatcc
     2521 ccgccatttt gatatcaagt aaaatcagat catatttttt gctgttaact aactttaatc
     2581 cttcaatgcc attttcagca tcatccactt tgtagccctc gtattcaagg atatctctaa
     2641 gcgtgttacg gatacttcgt tcgtcatcaa cgaccagaat ttttgtcatt ttaaatctcc
     2701 attaatagtt gctgtgtggt tgcaaaaata atgtaactta attagagttc ggtatttaaa
     2761 ggttggtgtt tttcattggc taattatttt tttttgatga tagtctttga ccgaattaat
     2821 ttagattcaa cataaaccaa cgttatttcg agggtgcttt cttaagatca atagtttagg
     2881 ctaaatgctt tttcaattgg ttaattatag attaaattat ttttgcctaa aatctttgtt
     2941 atgaatttta aagttggcga taaggttaag tttttaaatg agagtggggg cggggagatt
     3001 aaaaagatta tcagtagtaa tttagtaagt gttcagattg acgatggttt tgaaattcct
     3061 acgttaatca ctgagttgat aaaagtggat gaggagccgg aaactcctaa gatgatgttt
     3121 actcagcaac aatttcggcg tcagccgccg gctccgacag aaacttctga cgatgatgaa
     3181 aattattatg aagagggacg ggtttcacca ttggaaattt tccgttcaag gggcgatgat
     3241 aagaaaggta tttatttggc ctttgtgcct cacgaccaaa agtggctgct taccggtctt
     3301 ttggatatct atttagttaa ttattccgaa tacgaagccc tttttagctt gtttttacga
     3361 aagcctaatg gcgcttggga aggagtagat tatgacgtta taaaacaaaa cagtaaaata
     3421 ttgctcgaca ccataaagcg tgaagagatt gaaaagtgga gctatggaat ttttcagatt
     3481 ctttttcaca aagatttatc atcaaaagtg ttgagcccgt taagcagtgt ttttagcttt
     3541 aagcctacca aactgtatca tgaaaatgcg tatcagaatt ctgtttttat cagtgggaaa
     3601 gcatttctgc tgatcgtgtg tgaattagcg ctgcatccag tcgttgctgt atcggagttg
     3661 aatgagaaat ttgatgaaga ggttttgcag caaacggcta agatgaaaaa acctgaggag
     3721 ttcattaata aacataagac aagccctcgc gaggcagtgg tggatcttca tatagaagag
     3781 ctgttggata attattcaaa aatgaccaat catgaaattt tgaattacca attgaattat
     3841 ttcactaaat gccttgagag tggcgttaat aactatttga ccaagatcac ttttattcat
     3901 ggtattgggg atggtgtgtt aaaggcgcga atgttggaaa ttttgaaaga gtatgataat
     3961 gtgcagacca aggatgcttc aatgcatcaa tttggatatg gcgcccttga cgttttgata
     4021 tggcacagcg cacaagttta gaaaaatctt tgataactcc gctgtttggt caaagagctt
     4081 attttctacg ataaaatttt tgtaaggtcg aaaaaatcgt taatggttaa ttgctccggt
     4141 cgtttattaa acaccggata atcatcagga ttgatcttgt cagtccaaag tttccgtaat
     4201 gagtttttaa gtgtttttcg gcgttggtta aatccagttt taacaatgtt gaaaaagagt
     4261 ttctcatcaa tgtcaggata tttttcggct gtgcgttcca aacgaataac tgccgacttt
     4321 acttttggag gcgggaaaaa aacctgttca tttaccgtga aaaggtactt tattttataa
     4381 tatgcaccta atattactgt tagcactcca taggatttgt tgcctggctt ggctgcaagc
     4441 cgctcagcta catctttttg caacatacat acaatttcct gaataatagc ttgatagttg
     4501 ataattttaa aaaatatctg actggatatg ttatacggaa agttgccgat aatggaaaag
     4561 gacccttcaa acaagctatt aagatcgcat ttcaaaaaat catcatttat tatcctattc
     4621 tctaattctg gaaactcttt ttcaagaaag ccaacgacct ctttgtcaat ttcgattgct
     4681 ttaaaatctt tacagacagg aataagatac tttgtcaata cacctgttcc aggtccgatt
     4741 tccaaaatcc gttcgttggc tttcagactg ctaacaattt tccttgctat attttcatct
     4801 ttcagaaagt gctgtccaag acttttttta ggaacaactg attgcatagt tgcagtttag
     4861 gctattatgt tgaacgaaag caagaggttt ttattaatta taaataagga caaagaatct
     4921 ttttgtttta attggtcata tcgcctctta atatcctgaa tcgttttcgg gcttcaatag
     4981 tataaaggct acctggatag tcgaatagga ttttttcgta aagctcctta gctctaacaa
     5041 tatctttaaa atggttttcc atcatatcgg ctcttaacca cagagcatca tcagcgagga
     5101 ggtcatatgg atacattgta actaacttat ccaaaagatt gtcggcctca ttatatttcc
     5161 cctttttcat catgatctct gctcttttaa agagcacgtc atcattcaat gagtgccaga
     5221 gcgacagcat ctgtattgaa tcaagtgttg cccatgcttc atcatattta ttgcgataaa
     5281 aaagtaactc ggctcgtgcg aaaatgccaa gagcagtata agtactgtca agatcaaggt
     5341 tatcgcttat cagcaatgat agctccattg catcgttggc aatcagtttt gatgtggctg
     5401 ccttcaagac gtctaactgt gcctttgccc agccaaattc acctatataa tatgatagct
     5461 ttgcgttttg taactttgcc cgatgaccga tcggatcgtg tttaaaatcg tattcaacct
     5521 gtgagtaaag tagagtggca tcccacacat taccagaaaa tagcagtatg tcagccagtt
     5581 ctattttaca ttcggctttc atattttttg ctaccgaggg catctctaca gctttttgca
     5641 atagtgaaat tgcctgatca atatcgtcaa gatagaatgc ttgtagatga gccatgtaac
     5701 gcattatgga gacagtcgag gtgttttgtc caaactcgtc caatgctttt tgatactcat
     5761 ttttgagctc aatcaaatcc ttatgggtat agtcatatcg tgtggttact tttaggtatc
     5821 tggcgttgag taatccaata cgcgagtcga gataaaaagg agatgatgtt cctttttcca
     5881 acagatagct atatgcattt atggcaacat cataggattc atttgacatg caaagattag
     5941 caagctcgta aatcctttgt ccttgttcct gaaaacgaag gtcgagtgag cgcgcctgaa
     6001 tgaatgccat ttcgaagtcg tgctgctgaa ccgaatgcca gataagcatc tccgaaaaat
     6061 aagtcttgtc aggcgctcgc tgaatcctgc gaagcaattc ttttcgcagc atctcattct
     6121 tttcgccttc ggtatcatcg ctaagcgctg attgtaaccg tgattttacg gtttcaattt
     6181 tggattgatc aatatcaagc aggtcaaggt actcattaaa catcatttca tagttctgct
     6241 gccgctgata gataaaagca agatcaaggt tgaaagggtg atccggaacc aatattttac
     6301 cctttaggta cgtctttata gcccaatcgg attgccctct ataattaaat gcgttggcca
     6361 gatcacgaat ttggtttgca gtcgaaccaa tattttcgaa ggctttatca aagattttat
     6421 ttgcctttac aagttctccg gaaattaaat aaatatagcc ttcgtcaaca tcataccgaa
     6481 ggttgttcgg attactcttt tgttgttttg ttattgcttt cagggctttc ttataatcct
     6541 gaagctcgat gaggcaatag atgtaataat tgtaataaaa atgggtgcct ttctcattaa
     6601 atagctgttc gtaaagtaaa acggcttttt cgaaatcccg attccggacg tattcggcag
     6661 caagtttctc tttgtccggt tcctgtttct tgtttattgc agtctcggcc tttggaaatg
     6721 cagggatatc ctgtccgtaa agtggagcat atcctaaaaa taataatatg aaaggtatat
     6781 aaaaatatga tttcattgca ttaactgaca ttgagataat acaaaccatt aatgtacagg
     6841 tttagtattt tgttcactac tttgactttt tgtaaaaggg gtgaagccgc taatcaatca
     6901 ttttgaaacc ggtatatgga attaatgagt cggatatttt tattcctgtt tcattttgat
     6961 tgttttccaa taaagctgcg actattcgtg gtaaagccag agcgctgccg ttgagtgtat
     7021 gtgcgagttc ggtcttgcca tttttgtttt taaatctcaa tttcattctg ttagcctgaa
     7081 aactttcaaa attggaaacc gagcttacct ctagccatct cttttgtgct gccgaataga
     7141 cttcaaaatc gtacgtcatg gctgaagtaa aacttatatc ccctccgcag agcttaacaa
     7201 tcctgaaagg cagctccaat ttaacaagta atgtagatac atattcgcgc atctcttcca
     7261 gaacctgata agatgtttcg ggtgtggcta tttgcactat ctccaccttg tcgaactgat
     7321 gcaggcggtt taagcctctc acatcggcgc cccacgaacc agcttcgcgt cggaaacaat
     7381 tggaataggc aatgttttta ataggcaagt cgctctcctt taatattgta tctcggtaaa
     7441 tattggtaag tggcacttcg gcggtaggga tcagataaaa tttatcaagt cctacattat
     7501 acatctgacc gtctttatcg ggtaattgac ctgttccgta tgctgacgct tcatttacaa
     7561 gcaatggtgg ctgaacctca gtgtatccgg cttttagtcc ctcatcaaga aagaagttga
     7621 tgagtgcacg ttgtaattta gctccttttc cacgataaaa cggaaaaccg gcccctgtaa
     7681 ccttattacc aagctcgaaa tcaatgatgt catacttttt aattaaatcc cagtggggta
     7741 gggctgaagg agagagggtc ggtatttttc caaaactaaa gacaatctca ttatcttcgg
     7801 ctgttttacc ctgtggaact tgatccccgg gtatgtttgg tatttgtaat aaaagcgaat
     7861 ttatttcatt gttaagcaat tccaggtcgt tagccattgt tttggcctca gcttttaaag
     7921 taacggaacg ctcctttagc tgtgttgctt gatcttgctt gccgctttta ataatttctc
     7981 caatagtttt ggctatttta ttggcttctg aaagagtcgc atcaagttta acctggtttt
     8041 ctcgtttttt ttcatcaagc gccaaaatat ttgtaatcaa ttcggaagca tcaaaatttt
     8101 tgaccttcaa ccgttcaatt acaaaatccg gattttgacg gaggatatgt atctgtaaca
     8161 taatgtgatt attgttttat cctaaacaaa aaatctcccg tttagtaaaa aaaaacagga
     8221 gattctgatt agttaatttt tttgatacta aattagttgt cggtattttg atcttcaaaa
     8281 gggattactg atacaaatga tttattattt tttctccttt tgaattctac tgttccgtca
     8341 acagttgcaa agagtgtatg atcttttccc attcctacgt taacacctgg attatggact
     8401 gtgccgcgct gcctgacaag gatattacct gctttggcaa actggccgcc gtaaatcttt
     8461 acacctaatc gcttgctatg cgattctcga ccgttggacg aactgcctgc gcctttttta
     8521 tgagccatag tatttaaatt tttgagtccc ctctaaaatg gaacgattat taaaagtgat
     8581 aaaatattga caagtgataa aaatacagtt ttagaaaatt gagggggcaa atttggttga
     8641 aaatttattt gcaaataaaa tttgctctta aaatcatcct ttttcaccct caatttcatg
     8701 gttttagttg ttattttata gattcattaa acatttctcg tgtaaatttt ctctcgtaaa
     8761 gatttgaata ataactctac aagaaacatc attttattga taatcaatta tttatagctt
     8821 tgtgaacaaa catgttttta ataaagtgta aatctgctac tccgactttt tagagtggat
     8881 ttaattttga tgtttaacaa ttttattaac caacagttat ctcgtcaatt ctgatacgag
     8941 ataaatactg ccgatgaccg gtctcttttt gaaatccctt cctgcgtttc tttttgaaca
     9001 caattacttt gtctccttta aggtgagaaa ctattgtggc agaaaccgat gcgccatcaa
     9061 cgaaaggttg cccaatacta atgttaccgt tattatcaag taacaacact ttgtcgaaag
     9121 taacggtatc gccctctaac ccttcaaggc gatgaacgta aatttgctta cccttctcga
     9181 ttttaaactg ttgcccagct atatcaacta ttgcgtacat tgtttcggtt taaagtattt
     9241 ttaattaaaa atcggggcgc aaaagtataa ataattcttt aattccaaat tattggaata
     9301 attatttttt ttatttcaaa aaaaaactac ccacatatta aaaattgttt atttttgacg
     9361 aattgttggt ttgactgtga ataacttttt aaaaaatatt ttcaaatagg agtatatttc
     9421 aaagaatatt ttatttttgc acttgtaaat tacgaaaata gagtactgaa atcaaattta
     9481 gtaacatgga acaagcgaac attaacgaaa taaagccacc ataactaatt ggattaaaga
     9541 aacttaacga tttttttatt ttcttaagat aacatttaac ataataataa ttaaacaaag
     9601 tcaaatattt taaattaatc aaaacaaata acctatgaaa caaattacat gttttctgtt
     9661 ggtgtttttc tttatgattt catcaactgt gttgttttca cagcaaccta ccagcttaga
     9721 taagctgaag atggagcaaa tggagaaata tgttaactcg gataatggac ctagcgtaaa
     9781 agctacagcc cagccggttg aaggtgaaca ttatacctcc tatgctactt attacccctc
     9841 gcgttcggag ttgtataaca acggtccgtt tattactcat cctgggggtg gggccggagg
     9901 caaagatgca agtgcagttc aaactgacct tggcatgagt gcttatggtc caaatgtgaa
     9961 tcataatatg tcgcccggta actattatta tttagctgat gattttgagg tagtaggaac
    10021 ctggaatctc gagtcaatta aattttatgg ttatcagact ggttctggca ctacttcaac
    10081 ctttaccggt atttatgttc agatatggga tggagcgccc aatgctggcg gcgctgtggt
    10141 ttggggagat ctgacaacta acaggatgct atctactcag ttttctgatg tttaccgtgt
    10201 cttagatact ggcttgacca ataccgaccg cccaattatg gagatcatag cagatgtgtc
    10261 aggctgtact cttaattctg gtacctattg ggtacagtgg gggcttaccg gatctgctgc
    10321 atctggtcca tggggagttg ctattacaat actaggccaa actaccacgg gcaatggcct
    10381 tcaggctata tctacaggat ggcaaaattg gcaagatagt ggaacatcta ctggtcaagg
    10441 aggagtattt atcattgaag gtactgctgg ctctcagcct acaaaagatg ttggagtaca
    10501 a
//
LOCUS       DAOOAI010000079        10239 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_8196_length_10239_cov_5.696485, whole genome shotgun sequence.
ACCESSION   DAOOAI010000079 DAOOAI010000000
VERSION     DAOOAI010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10239)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10239)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10239
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8196_length_10239_cov_5.696485"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1356)
                     /gene="lpdA"
                     /locus_tag="PK345_06545"
     CDS             complement(<1..1356)
                     /gene="lpdA"
                     /locus_tag="PK345_06545"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HPX34707.1"
                     /translation="MNYDLIVIGSGPGGYVAAIRASQLGMKVAVVEKESLGGICLNWG
                     CIPTKALLKSAQVFNYMKKAAEYGIKLDHTPEVDFSSIVKRSRDVAAGMSNGIAYLFK
                     KNKIDILSGSGKVKPGKIVEVTDNEGKKQEYSANHIIIATGARSRELPNLKQDGKKII
                     GYRQAITLDKKPESMVVVGSGAIGSEFAYFYNSIGTQVTLVEFMPNVVPLEDEEVSKQ
                     LARSFKKAGIKVMTNSTVESVDTKGERCKVTIKTKKGEEIVETDIVLSAVGITTNLEG
                     IGLEEVGINYEKGKIIVDQFYRTNVDGYYAIGDVVSGPALAHLASHEAITCVEKIAGK
                     DVQPIDYGNVPACTYTIPEVASVGMTEKQAIEAGYEIKVGKFPFSASGKASAAGNKEG
                     FVKVIFDAKNDKWLGAHMIGDNVTEMIAEAVVARKLQTTGEEIIKTIHPHPTMSEAIM
                     EA"
     gene            1645..2178
                     /locus_tag="PK345_06550"
     CDS             1645..2178
                     /locus_tag="PK345_06550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34708.1"
                     /translation="MKTKILFLIIAFSGMLFLSGCEDTLDVTENFIYELEVKVFSEDH
                     TFSHAVLVDMKNEQDLIKKYGDKIKNIQIEEVKYWLTEFDGSETQQIVQATLSVLNEN
                     DDVTVITTVKDQLLQPLVNTPTKLDINQEGIDKMASLIENSPHQFKLSFTSTVNETPL
                     NFTLKFSFNIKMTANPL"
     gene            2441..4321
                     /locus_tag="PK345_06555"
     CDS             2441..4321
                     /locus_tag="PK345_06555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009018130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HPX34709.1"
                     /translation="MNLLTVENLTKSFGEKLLFENISFGIEVGQKVALIARNGAGKST
                     LIRILAGEEIADEGKITMGNDITISYLPQNPEMDEDQSVLDYLFDSDNALVTLIKQYE
                     ELIAQKTVNQQLLGQVINRIDELHAWDFEAKIKEILGKFDINDLNQKIGALSGGMRKK
                     VALSKALIEEANFIILDEPTNHLDITMIEWLENYLDRQNLSLFIVTHDRYFLDKVCNV
                     IIELDGGNIYRYKGNYAYFLEKKAERLAVEMAGLEKARSLYNTELEWMRRQPQARATK
                     PKAREDAFYDLEEKAKQKSKVETREFNVNMQRLGGKILELNYISKKYGGQKILEDFTY
                     TFNRGDRIGIVGKNGCGKSTLLRIIMGEIKPDKGKIVRGQTVEFGYFQQEGLPINEDK
                     RIIDIVKEIGEQIEIKKGSMSPVQFLSYFNFHPDVHYNYFSKLSGGEKRKLYLLTVLL
                     RNPNFLILDEPTNDLDIETLNKLEEFLLNYEGCLLIVSHDRYFMDHIVDHVFVFQGDG
                     KVKDYYGNYSEFYRQWKIEENRQRKKNTKDKPIKKPDNRGKKLTNKPTYKQVKEYETL
                     EDEITQLELRKSNLIDKMNQGEGTHEDYRIWSEEVGEIVRIIDQKTDRWLELSEIIER
                     VE"
     gene            complement(4488..5480)
                     /locus_tag="PK345_06560"
     CDS             complement(4488..5480)
                     /locus_tag="PK345_06560"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPX34710.1"
                     /translation="MKKLLVIRFSSIGDIILTTPIVRCLKKYFPECEIHYLTKQAFKP
                     ILKANPYITKIITIDKRINEAIPILRAEKYDYVVDLHKNFRSIGVRLALGGKSSSFPK
                     LNFKKWLLVNFKINLLPNIHIVDRYFEAIESMGVKNDNSGLDYFIPEMEPSAFDLIPE
                     SHRSGYVAMVIGGKHKTKQMPVELSVRLVKEISYPVIILGGPDDSTEGSIIESEAGNQ
                     VWSACGKLSINQSAMLIKQAKVVITNDTGLMHIAAAFKKQIISIWGNTIQEFGMYPYI
                     PSNKDLYRIFEVNGLDCRPCSKIGFDKCPKKHFNCMMKQDINAIAMCTSKMWEN"
     gene            complement(5597..6949)
                     /gene="guaD"
                     /locus_tag="PK345_06565"
     CDS             complement(5597..6949)
                     /gene="guaD"
                     /locus_tag="PK345_06565"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: protein motif:HMM:NF006679.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HPX34711.1"
                     /translation="MLKGYRGRLFDFKSDSDVSCLKEKYRFFDDGLLVIEEGKIVASG
                     DYSQVINNLNQEIDIESFGECFILPGFIDAHVHSAQTQAIASYGNQLLDWLEGYIFPN
                     ERQFEQPEFALEHTRFFFRQLLKNGTTTAAIYPSIHDVSVEVVFDIASELNMRVITGK
                     TWMDCNAPDFLLEDPVQSYESTQKQIEKWHEKGRLHYAITPRYAITSSPRSFELAASL
                     LKDYPRLYLQTHVSENRQEVKVVNHHYYNYKGYLDVYDSFGLLTPRTLLGHGIYLTDI
                     EFEKIAETKARIVHCPSSNLFLGSGLFDYHKILKYGINLAIGSDVGGGTSFSMLQNLN
                     DAYRISALKNNTCDDNPHSFDTLAALDAFYLITLGGAKTLSLNDMIGNFDPGKEADFV
                     VIDPLLNEVLTYRIKNADSIEDLLFALMILGDERIIKATYLMGNRVYNNNVYFEKSDV
                     "
     gene            complement(6995..7738)
                     /locus_tag="PK345_06570"
     CDS             complement(6995..7738)
                     /locus_tag="PK345_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HPX34712.1"
                     /translation="MKKIVIEIGAYNIESALIAQRYGADRVELCSGFAEGGLTPGPGM
                     IKLARKLLSIELYIMIRPREGDFLYTDHEFETMIEDVIFAKNAGADGIVTGLLLADGN
                     VDEKRTRMLVEIAHPMRVTFHRAYDMTNNPYKALETIISTGTTRILTSGQKPDAIQGA
                     GLIAKINEQAAGRIKIMAGCGIKDYNVSEIIKNSGVKEVHLSAKGYTNSKMTFRQPDL
                     FMGTPESDEYMNLLTDGKMIARIVEILKN"
     gene            7893..9635
                     /locus_tag="PK345_06575"
     CDS             7893..9635
                     /locus_tag="PK345_06575"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009138127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HPX34713.1"
                     /translation="MTFNRPFLVFIILIFASATLYPCTSYLVTPGASFDGSSMISYAA
                     DSHIRYGELYYFEGGAKPEGSYFEMYCRGTHKPLAKIPHPPFTYSVVGFINEKQVAIG
                     ETTFGGRPELRDTTGLMDYGGLMFLALQRAATAREAIMVIGKLVEEYGYYSSGESFSI
                     SDPYEVWIMEIVGKGTDLQYDKKNKKYVNSNKGAVWVAVKIPDGYISAHANHARIMNF
                     PLEDGVSSISSKNWDKIELPDVGVIYAHDVITFARKMGYFNGDDKDFSFSDVYAPIDF
                     GAARFCEARVWSMFRKVNSSMDIYENYAMGYDLSNRMPLYIKPERKLSVKDLIEFKRD
                     YLQGTKYDMTQDIGAGPWGLPYRWRPLTWEVDGKTYIHERVTVTQQTAFSFITQSRRN
                     YPDHIGGIIWFGVDDANSTVYVPMYAGIRRAPEAYAEGNGSILEYSESSAFWTFNKVS
                     NFAYLRYNLMLPDIKKVQSELQEQFIAYVPAIDKAAADLYQKDPEKGRDFITDFSVKM
                     GNYTVERWEELFRFLMVKFLDGNIKKEENGQFLTRKYGKYPIVIHPEYPEWWLKLIVE
                     TTGDKLLYQNDNKE"
     gene            complement(9696..10166)
                     /locus_tag="PK345_06580"
     CDS             complement(9696..10166)
                     /locus_tag="PK345_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HPX34714.1"
                     /translation="MIDQITHFNIRVYGLIFNEKKELLLTDEFRLGIRMTKFPGGGLK
                     FGEGTIDCLKREIMEEIGQDIEIVRHFYTTDYFRPTMHLKTTQQLISIYYCVKLLNPN
                     TLKVSDKPFDFKDKEGAQSFRWVSLERINEKDLTFPIDKHVLQLLKAENRDLPR"
BASE COUNT         3189 a         1906 c         1915 g         3229 t
ORIGIN      
        1 agcctccata atagcttctg acattgtcgg gtgcggatga atagttttga ttatctcctc
       61 tccggtagtt tgaagtttac gtgcaactac agcctctgct atcatttcgg taacattatc
      121 gccaatcatg tgtgcaccaa gccatttgtc attttttgca tcgaatatta cctttacaaa
      181 accctctttg ttgccagcgg cgcttgcctt acctgatgct gagaaaggaa attttccaac
      241 ctttatctca taaccggcct ctatggcttg cttttccgtc atacctaccg aagccacctc
      301 tggaattgta taagtgcagg ccggaacatt gccgtaatca attggttgta catctttgcc
      361 ggcaatcttt tcaacacagg ttatagcttc atgcgaagca agatgcgcaa gggcgggtcc
      421 actaacaaca tcgccaatag cataataacc atctacgttg gtacgataaa attgatcaac
      481 aatgatctta cctttttcat aatttatacc cacctcttcc aagcctattc cttcgagatt
      541 agttgtaata cctacggccg acaaaacaat atcagtctca acaatttcct cacctttctt
      601 ggttttgatc gtaaccttac atctttcgcc ctttgtgtca actgattcaa cagtagaatt
      661 ggtcatgact ttgataccgg cttttttaaa tgaacgagca agttgtttag aaacctcttc
      721 atcttcaagt ggaacgacat taggcataaa ttcaaccaaa gtaacctggg tgccaatcga
      781 attgtagaaa taggcaaact cagaaccgat agccccagaa ccaacaacta ccatgctctc
      841 aggcttttta tcaagagtta ttgcctgacg gtaaccaata attttttttc catcctgttt
      901 aaggttaggc agttcccggg aacgtgcgcc cgttgcaatg atgatatgat ttgctgaata
      961 ctcttgcttt ttaccttcgt tatcggttac ttccactatt ttgcctggtt ttaccttacc
     1021 cgatccggag aggatatcaa ttttgttttt tttaaagaga taggctatgc cattactcat
     1081 cccagcagca acatctctgc ttcgttttac aatactacta aaatcaactt cgggtgtgtg
     1141 gtcaagtttt attccatatt ccgcagcctt tttcatataa ttgaatacct gcgcactttt
     1201 aagaagagct ttggttggaa tacatcccca gttgaggcaa ataccgccta atgattcttt
     1261 ctctactaca gctaccttca ttccaagctg tgaggctctt attgcagcta catatccacc
     1321 aggaccgcta ccaatgacta ttaaatcgta attcatatct ttatctctat ttaatttgcc
     1381 gcaaaagtac gtattataaa ttatgagttt ttcttagagt agatttttga gttgttgaaa
     1441 attgagctgc acttaaaaag tggatagaaa aatcattaat tataagtttt tgtgccgtga
     1501 aaaacaattt ttattgtttg aaatattgtt caatgggatt acaccatctt attaagccac
     1561 atttttcaat cagttttgaa gtttgtagga atagattatt tttgttatgg aaataaaatt
     1621 attaactaat tagtaaaatc tgttatgaaa acaaaaattc tctttcttat cattgctttc
     1681 tccggtatgc ttttcctttc aggatgcgaa gatacgcttg acgttactga aaactttatc
     1741 tatgaattag aggtgaaagt cttttctgaa gatcacactt tttctcatgc tgtattagtg
     1801 gatatgaaaa atgaacaaga tttaattaaa aaatatggcg acaaaatcaa aaacatccag
     1861 attgaagagg taaaatattg gttaacagaa tttgatggtt cggaaacaca acagattgtt
     1921 caagctactt taagtgtact taatgaaaat gacgatgtta ctgtgattac tactgtaaaa
     1981 gatcaattgc tgcagccatt ggttaataca ccaaccaaac ttgatattaa tcaagaagga
     2041 attgataaaa tggcttctct tatagaaaac tcaccccacc agtttaagct ctcttttacc
     2101 agcacagtca acgaaacccc tttgaatttc actctaaaat tcagctttaa tattaaaatg
     2161 actgccaatc cgctataaac ggagtctttt ttaaatttct taccataaaa aagctgaatt
     2221 gctttcaggg cgataattca attgctatct catgtttata agttatagca ttttaattta
     2281 gataccaaat agagcattat tatcgccaac aataaagagc taactttaag cagttagata
     2341 agattatgct ttggtaataa tgcttagtaa taaattgctc tgataacgct tgggttttgt
     2401 ttgcttactt tgcaaaaaat ttactcatcg ttgatattag atgaatttac tgactgttga
     2461 aaatcttaca aaatcatttg gcgaaaaact tctttttgaa aacatctctt ttggtattga
     2521 ggttgggcaa aaagtagctt taattgcccg caatggcgca ggtaagagca ccttaatacg
     2581 gatattagcc ggcgaagaaa tagccgatga gggtaaaata actatgggta acgacattac
     2641 tatttcgtac cttcctcaaa accccgaaat ggatgaagat cagtctgttc tcgattatct
     2701 tttcgattcc gacaacgctc ttgttacact aattaaacag tacgaggagc taattgctca
     2761 aaaaactgta aaccagcaat tgttaggaca ggtgatcaat cggatagatg agttgcatgc
     2821 atgggatttc gaagcaaaaa tcaaagagat tttaggtaaa tttgatatca acgatttaaa
     2881 tcaaaaaatt ggagctctat ctggtgggat gcgcaaaaaa gtagctcttt caaaagccct
     2941 catcgaagag gcaaacttta ttatacttga cgagcctacc aaccatcttg atattaccat
     3001 gatagaatgg ttggaaaatt atttagaccg gcagaatctg agccttttca ttgttactca
     3061 cgatcgttat ttcctcgata aagtttgcaa tgtaatcatt gagctcgatg gtggaaacat
     3121 ctatcgttac aaaggtaatt atgcatattt tttagaaaaa aaagctgaac ggcttgcggt
     3181 ggaaatggct ggattggaaa aagcccgttc gctctataat actgaattag aatggatgcg
     3241 ccggcagccg caagcgcgag ccacaaaacc aaaagcaaga gaagatgcct tttatgattt
     3301 agaggaaaaa gcaaaacaaa agagtaaagt cgaaaccaga gagtttaatg taaatatgca
     3361 acgcctcggc gggaaaatac tggaacttaa ttatataagc aaaaaatatg gcgggcagaa
     3421 gatactcgaa gattttacct atacatttaa ccgtggcgat aggatcggga tagttggtaa
     3481 aaatggctgc ggtaagtcca ccttgttacg aatcatcatg ggcgaaataa agccagataa
     3541 aggaaaaatc gttcgtgggc aaacagttga gtttggatac ttccagcagg aggggctacc
     3601 cattaatgaa gacaaacgaa ttattgatat tgttaaagaa attggcgaac agattgagat
     3661 aaaaaagggc agcatgtcgc cggtgcaatt tctcagctat tttaacttcc atcctgatgt
     3721 acattataac tatttctcaa aacttagcgg aggcgaaaaa cgaaagttat accttctgac
     3781 cgtcctttta cgaaatccaa attttctaat tttagacgaa cctaccaatg atcttgatat
     3841 cgaaacactt aacaaactcg aagaatttct actaaattac gaaggctgct tgctcattgt
     3901 atcgcacgat cgttatttca tggatcatat tgtagatcat gtatttgttt ttcaaggaga
     3961 tggtaaggta aaggattact atggaaacta ttctgaattt tatcgccagt ggaagattga
     4021 agaaaataga cagagaaaga aaaatacaaa agataaacct ataaaaaaac ctgataatag
     4081 gggtaaaaaa cttacaaata agccaaccta taaacaagtg aaggaatatg aaacacttga
     4141 agatgaaatt acgcagttgg aattaagaaa atccaatctg attgacaaaa tgaatcaggg
     4201 cgaaggtact catgaagatt atagaatatg gtcagaagag gttggggaaa ttgtccgtat
     4261 tattgatcaa aaaactgacc gctggttaga gttaagtgag ataattgaaa gggtagagtg
     4321 aatgtccgtt gaactttaac gatacagtta aatccagatt tattgagaat atcgtacttg
     4381 gtgtttttta agaaaaatca aaatgatgag atgttttctt taaaccgata caaatctttt
     4441 aactgtattc tttcttaggt aggatatatt tccaattgtt attcaaatta attttcccac
     4501 attttgctgg tacacatagc aatggcgttt atatcctgtt tcatcatgca attaaaatgt
     4561 tttttagggc atttatcgaa accgattttt gagcatggtc ggcagtcaag gccattcacc
     4621 tcaaaaatcc tgtaaagatc tttattagat ggtatataag ggtacatccc aaattcctgt
     4681 atcgtattgc cccaaatgct gattatttgt tttttaaatg ctgctgcaat gtgcatcaga
     4741 ccggtgtcgt tggttatcac tactttagct tgcttaataa gcattgccga ttgattgata
     4801 cttagcttgc cacatgccga ccaaacttga tttcctgctt cactttcgat aatagagcct
     4861 tcggttgaat catcaggccc gccaaggatg attaccggat aagagatttc ttttacaagc
     4921 cttaccgata gttcaaccgg catttgtttg gttttatgtt ttccaccgat aaccatcgcc
     4981 acataaccgc tgcgatgtga ttcaggaatc agatcaaaag cagaaggttc catttcaggt
     5041 ataaaataat cgagaccact attatcattt tttacgccca tactttcaat cgcctcaaaa
     5101 tagcgatcaa caatatgaat attggggagc agattgattt taaaattaac aagtaaccat
     5161 tttttaaaat tcagcttagg aaaagaagag ctttttccac caagagctaa acgtacacca
     5221 attgacctaa agtttttatg aagatcaaca acgtaatcgt atttttcagc cctcaggatt
     5281 ggaatagctt catttattct tttatcaatg gtaattattt ttgtaatgta gggattggct
     5341 ttaagtatgg gtttgaatgc ctgtttagta aggtaatgta tctcgcattc gggaaaatac
     5401 tttttaaggc agcgtactat tggagttgtc aatataatgt ctcctattga actgaatctt
     5461 atgacaagga gttttttcaa ccgaaaaatt tttgttaaac aattaaaatc acaactttga
     5521 tcattttaat atgatacggt taacgtatgg ttatggttta cattatttgt cctggcgtta
     5581 tttggatact attgttttac acatcacttt tttcaaagta cacattatta ttgtacacac
     5641 gatttcccat cagataagta gcttttataa tacgttcatc gccgagaatc attagcgcaa
     5701 aaagtaaatc ttctattgag tcggcatttt taattcgata agtaagcact tcgttaagca
     5761 aaggatcaat tacaacaaaa tcagcctctt taccggggtc aaaattaccg atcatatcat
     5821 ttagtgaaag cgttttggcg ccgccgagcg taatcagata aaaggcatct aaagcggcaa
     5881 gtgtatcgaa actatgagga ttatcatcac atgtattgtt ttttaatgca gagattcggt
     5941 atgcgtcatt tagattttga agcattgaga aacttgtacc tccgcctaca tcgctaccta
     6001 ttgccaggtt tatgccgtat ttaagaattt tatgataatc aaataagccg ctaccaagga
     6061 aaaggtttga ggatgggcag tgtacaattc tggcttttgt ttcggcaatt ttttcaaact
     6121 caatatcagt aagataaatc ccatgtccta aaagtgtacg tggggttaat aggccaaaac
     6181 tatcgtatac atctaaatag cctttatagt tgtaataatg atgattgacc actttgactt
     6241 cctgcctgtt ttctgatacg tgagtttgaa gatacaaacg aggatagtct tttaatagtg
     6301 aagccgctaa ctcaaatgat ctgggagatg aagtaatagc gtagcggggt gtaatagcat
     6361 aatgtaatcg tcctttttcg tgccactttt caatttgttt ttgagttgat tcgtaagatt
     6421 gaaccgggtc ttccagcaaa aaatccgggg cattacaatc catccatgtc tttccggtaa
     6481 taactcgcat gttaagctca cttgcaatat caaacacgac ttctacagat acatcatgta
     6541 ttgaaggata tattgcagca gtagtagtgc cgtttttcag caattgcctg aaaaaaaagc
     6601 gggtgtgttc caaagcaaat tcaggttgtt caaattggcg ctcgtttgga aagatatagc
     6661 cctcaagcca atcgagcaac tgattaccat aagacgcaat agcttgcgtt tgagccgaat
     6721 gaacatgagc gtcaataaaa cctggaagga taaaacactc tccaaaggac tcaatatcta
     6781 tttcctgatt taggttatta attacttggc tataatcacc cgaagcgaca atttttccct
     6841 cttctattac cagcaatcca tcatcaaaaa aacgatactt ctctttcagg cacgaaacat
     6901 cagaatctga tttaaaatcg aaaagcctac cccgataacc ttttagcatg atcaaaaatt
     6961 tgaatccaaa attaggttat ttctccaata gagtttagtt ttttaatatc tccacaattc
     7021 gggcaatcat ttttccatca gttaacaagt tcatgtattc atcactttcg ggagtaccca
     7081 taaagagatc cggttgtcga aaagtcattt tggagttcgt atatcctttg gctgaaagat
     7141 gaacttcctt tacgccacta ttttttatta tctcgctaac gttgtaatcc tttatcccgc
     7201 agcctgccat tatttttatt ctgccggctg cttgttcgtt tattttcgca atcaaaccgg
     7261 cgccttgtat ggcatcaggc ttttgaccag aggtaaggat tcgtgttgtg ccagttgaaa
     7321 taattgtttc taatgcttta taaggattgt tggtcatgtc atatgcacga tgaaaagtta
     7381 ccctcatcgg atgtgcaatt tcaacaagca ttcttgttct tttctcgtca acgtttccat
     7441 cggcaagtaa tagacctgta actattccat cagcgccggc attttttgca aaaatgacgt
     7501 cctctatcat ggtttcaaac tcatgatcgg tataaagaaa gtcgccctca cgcggtcgga
     7561 tcattatata aagttctatt gaaagcagct tacgggcaag ttttatcatc ccaggccccg
     7621 gtgttaaacc cccttcggca aagccagaac ataactcaac cctgtcggcg ccatagcgtt
     7681 gagcaatcaa tgccgattcg atgttgtaag cgccaatttc aataacaatc ttcttcaaat
     7741 taattttttt aagcaaaaat aataaattac cggcttgaaa gtaacaacta aaaaatagaa
     7801 gtcggacaaa tcgtttttta aaattttact ttgacgactc tttcatcgaa aatttatagg
     7861 tttgccgcct aaacaattat aatcgttctg atatgacttt taaccgtcca tttttagttt
     7921 ttataatttt aatttttgca tcagcaacac tttatccatg cacaagttat ttggtaactc
     7981 ccggagcttc ttttgatggt tcctcgatga tcagttatgc tgccgattct catattcgtt
     8041 atggcgagct ttattatttc gaaggaggcg ccaagcccga gggctcttat tttgaaatgt
     8101 attgccgcgg tacacacaag ccgttagcca aaattccaca tccacctttt acctattcag
     8161 ttgttggctt tattaacgaa aaacaggtag caattggcga aacgacattc ggaggcagac
     8221 cagaactgcg tgatacaaca ggactgatgg attacggcgg attgatgttt ttggcgcttc
     8281 agagggcagc cacagcacgc gaagcaataa tggtaattgg taagcttgtg gaagaatatg
     8341 gatattacag ctccggcgaa tctttctcaa tttctgatcc ttatgaagta tggataatgg
     8401 agatcgttgg taaaggaact gacttgcaat atgataagaa aaacaagaaa tatgtaaatt
     8461 ctaacaaggg agctgtttgg gtagctgtta aaattccgga tgggtacata tcggctcatg
     8521 ccaatcatgc tcgtattatg aattttcctt tagaagacgg cgtttcttcg atcagctcaa
     8581 aaaactggga taaaatagag ctccccgatg tcggggtgat atatgctcac gatgttatca
     8641 catttgcacg taagatgggt tattttaatg gtgatgataa agattttagt ttttcagatg
     8701 tttatgctcc gattgacttt ggcgctgccc gtttttgcga agcccgtgtg tggtcaatgt
     8761 ttcgtaaagt aaacagcagt atggatattt atgagaatta tgcaatggga tatgatttga
     8821 gtaatcgtat gccactatat atcaagccag aacgaaaact ttcggttaag gatttgatag
     8881 aatttaaacg cgattattta caagggacaa aatatgatat gactcaagat attggcgcag
     8941 ggccttgggg attgccatat cgctggcgtc cgctcacatg ggaagtggat gggaaaacat
     9001 atattcacga gcgtgttact gtaacacagc aaacagcttt ttcgttcata acacagtcgc
     9061 gacgcaacta tcccgatcac atcgggggta ttatatggtt tggagtggac gatgcgaact
     9121 caacggttta tgtcccaatg tatgccggta tcagacgagc gcccgaagcc tacgccgaag
     9181 gtaatggaag catactcgaa tattctgaaa gttccgcatt ctggactttc aacaaagtat
     9241 cgaactttgc ctatttacga tataatttga tgttgcccga tattaagaaa gtacagtcgg
     9301 agcttcagga acaatttatt gcttatgtgc ctgctattga taaagctgct gccgaccttt
     9361 atcaaaaaga cccggaaaag ggtagagatt ttataacgga tttttcagta aaaatgggta
     9421 attacaccgt tgaaaggtgg gaggagcttt ttcgatttct aatggtaaaa tttttggatg
     9481 gaaatattaa aaaagaagaa aatggccagt ttcttacaag aaaatatggt aaatatccta
     9541 ttgtcatcca tcctgaatat cctgaatggt ggcttaagtt aatagtagaa accactggag
     9601 ataaactctt atatcagaat gataataagg aatagccagt aaagcaaagc cccaggactt
     9661 ttgaggataa aatagctcaa ttaagccaag tttacttacc tcggcaaatc ccggttttct
     9721 gctttcaata actgaagcac atgtttatct atagggaaag taagatcttt ctcattaatt
     9781 ctttccaagc ttacccatct gaagctctga gcgccctctt tatctttaaa atcaaatggt
     9841 ttatcagata cctttaacgt attagggttt agtaatttaa cacaatagta aatactaatc
     9901 agttgttgag ttgttttaag gtgcatggta ggccgaaaat agtcagttgt ataaaaatga
     9961 cgcactattt ctatatcctg ccctatctcc tccatgattt cacgtttgag gcagtcaatt
    10021 gtcccttctc caaattttaa acctccgccc ggaaatttgg tcatccgtat cccaagccta
    10081 aattcatcag taagcaacaa ctcctttttc tcattaaaaa ttaaaccata aacccggatg
    10141 ttgaaatgag tgatttgatc tatcaattga ttattcgttt ttttaaataa gtattgtttt
    10201 attttaggat agactactcc ttccaatagc cttcacgtg
//
LOCUS       DAOOAI010000080        10106 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_8321_length_10106_cov_4.296388, whole genome shotgun sequence.
ACCESSION   DAOOAI010000080 DAOOAI010000000
VERSION     DAOOAI010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10106)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10106)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10106
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8321_length_10106_cov_4.296388"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..745
                     /locus_tag="PK345_06585"
     CDS             <1..745
                     /locus_tag="PK345_06585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013617289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium chelatase domain-containing protein"
                     /protein_id="HPX34715.1"
                     /translation="ILVGHITKDGNIAGPKILEHMVDTVLYFEGDRNYGYRILRTVKN
                     RFGSTQEIGIYEMCDNGLRQVSNPSELLISQRNENLSGIAIASTVEGLRPMLIEIQAL
                     VSTAAYGVPQRATTGFDPRRLNMLLAVLEKRAGFRLANKDVFLNIAGGIKVDDPAIDL
                     AVVCAVLSSNEDIPLNQRACFTAEVGLSGEIRSVNRLDQRINEAAKLGFEKIYIAKGG
                     LKKSDPKLRPEIEIIGLSSIEDLFRELFG"
     gene            1031..3553
                     /gene="feoB"
                     /locus_tag="PK345_06590"
     CDS             1031..3553
                     /gene="feoB"
                     /locus_tag="PK345_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006800479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HPX34716.1"
                     /translation="MNLCELKEGQKGFISKVKGRGAFRKRIIEMGFVKGQEVEVVKNA
                     PLRDPIEYRIMDYLVSLRRSESRLIQVVSERELVEDENGVGFNGVIDTEKLKKKIRDK
                     GKSIDIALVGNPNSGKTTIFNFASHSREKVGNYGGVTIQSKTATFKQSGYTFNIVDLP
                     GTYSLTAYTPEELFVRNHILKQNPDIVINVVDASNLERNLYLTTQLIDMDIKVVVALN
                     MFDELQAKGDKFDYAMLGKMLGIPFVPTIGSKGKGIHALFAKAIEVYEDKDPLVRHIH
                     INYGKPIQRSLLKIQDELWKNKSLTDIVSSRFYAIKLLEKDEIIQKELESLLNFEIIK
                     RVVDNEIRILENSFKEDSETLITDSRYGFIAGALKETFTQDKLKRQQKTETEIIDTFI
                     THKIFGFPIFLFFLWLMFYSTFTLGEYPMKWIEAGVGFVSEFVSKFMPDGMLKDLLVD
                     GVISGVGSVIVFLPNILILFFFITFMEDTGYMARVAFIMDRIMHRIGLHGRSFIPMLM
                     GFGCNVPAIMATRTIESRSDRILTILINPFMSCSARLPVYILIIGAFFPSNPTMMLLL
                     IYGIGVMIAAIIAIVFKKIFFRKKEAPFVMELPPYRLPTLKAILKNMWYKASLYLQKM
                     GGIILLAVILVWAAGYFPQNRQQMYEFDKMIGDTKQDFQSQLSEIDPTIETERFERLH
                     EEMNMAILKIDIDKQSYRLENSFIGKFGHFIEPLIRPLGFDWKMGVSLVTGAAAKEIV
                     VGTLGVLYHADDPGNHQSLIEKLRSQRHQSGPKAGQLVFTPVTAMAFLIFVLIYFPCV
                     AVVAAVKKETGGWRWAAFLALYTTVLAYLLSLLVYQVGILIY"
     gene            complement(3748..4758)
                     /locus_tag="PK345_06595"
     CDS             complement(3748..4758)
                     /locus_tag="PK345_06595"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX34717.1"
                     /translation="MKFFTFSGLLFSLVILVSCHSSEKNTARETDQPLNVEQLILRVS
                     EKINSNPNDDRLYIERANYFLTIDSIQSALRDITIAIDINDQDPNHYITLSEAYLALG
                     NPDRCIEGLEKALEIEPKNQEALLKKAQLFLIMKQYDKTYETIDELIKIDSYNPTAYL
                     TRGYALLEQGDTIKAIRNLQTTIDQKQDYFEAYIQLGVVYINLGNPLAADYLSNAIEL
                     RPNSPEAYYLLGMFFQENDEPDKAIEIYNNILAIEPQFTFALYNLGYIYLVYFKEYET
                     AAGYFTQAVELDPNNYEAYYNRGYCYELLGNKSEARIEYQKSLELKPNYELAIKGLNR
                     LD"
     gene            5226..6572
                     /locus_tag="PK345_06600"
     CDS             5226..6572
                     /locus_tag="PK345_06600"
                     /inference="COORDINATES: protein motif:HMM:NF014904.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-Fe] hydrogenase large subunit C-terminal
                     domain-containing protein"
                     /protein_id="HPX34718.1"
                     /translation="MKDFHHALKLEGELCIGCSKCMNICPTQAIRVRNGKAILMPNRC
                     IDCGECFRVCPVSAISIEQDDVAQIFNYKYRIALIPAVFTAQFPSKFSIADIYTALYE
                     LGFTDVFEVEHGAHILREEVKKIIDNPDFPKPLISTFCPAIVRLIQVKFPSLVHNLIL
                     LKPPLDISALYFRRSLAEEGVKEDEIGIFYFTQCAAKIAAIKSPAERVESPITGVINM
                     DSAFNKVYQYLEQNIHNKESIAESPDNLSPEAILWSLTTGETEYYSGRRIAIDGVHNV
                     ISFLEKLENEENPGIHFLELRACHEGCAGGVLANENRFLSAERMRNRAKAAVNNQDGD
                     FSNRINDYKDDLKELIKTAEVKPRSMLKLDENMSEAMRKMNLIRQIIQLLPMVDCGAC
                     GSPGCYALAEDIVQGRGMMENCIFIQRRYEQKGMLKNQDAIALMKKIWGENKFESK"
     gene            6589..6927
                     /locus_tag="PK345_06605"
     CDS             6589..6927
                     /locus_tag="PK345_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HPX34719.1"
                     /translation="MTVRELVEKLGLHVFSDQSGLENEIKSGYTSDLLSDVMGHSNQG
                     AVWITLQTHKNIMAIASLKDHAAIILVKGLKPDDDTLATSNEEKIPILGTDLQTFEIT
                     GKVYQLINKQ"
     gene            6939..7670
                     /locus_tag="PK345_06610"
     CDS             6939..7670
                     /locus_tag="PK345_06610"
                     /inference="COORDINATES: protein motif:HMM:NF014826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HPX34720.1"
                     /translation="MPVFSADLHIHTVLSPCGDLDMSPVNIVKRAKELQLDIIGITDH
                     NSTRHCPLIQQLAAKEGIFVLCGAEVTTLEEAHCLCFMPDLERLDKLQSYLDLYLMDF
                     MNDVNKFGYQVQVDEEETIVYEEPKLLIQSINRNINQVEEFTHSLGGLFIPAHINRPA
                     FSLISQLGFIPDDLNYDALEIYRMTDENFCNTYNYRKDTTLIRNSDAHQLNRIGAERN
                     FFNIEELNFQEIKLALLKQQGRFVK"
     gene            7667..8215
                     /locus_tag="PK345_06615"
     CDS             7667..8215
                     /locus_tag="PK345_06615"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX34721.1"
                     /translation="MTEISLHILDIVHNSIRANAKKINISITEDLKEDKYLLKIVDDG
                     KGISASTLKHVTDPFTTSRKTRKVGMGLPLLKHSADQTGGWLKISSEIGKGTEVTCCF
                     GLAHIDRPPTGNIASIIYQLLSAFPEIRFVYTHKTSNGEYLFDTDELKEALDNLPVTM
                     PEVRKFILEMIEENLREIKIAP"
     gene            8341..9540
                     /locus_tag="PK345_06620"
     CDS             8341..9540
                     /locus_tag="PK345_06620"
                     /inference="COORDINATES: protein motif:HMM:NF013237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HPX34722.1"
                     /translation="MKFDPANSIQDLLQLGEYGDVNPSVTDSATYTFKQAKTMLDTFK
                     GEAEGCFLYSRHWNPSNKYLADAMAAMEGTQAAWVTASGMAAITCAILQICSAGDHIV
                     SSRTTYGGTFAFFENYLPKLNIQVTMVDVTDLKKVEAAIQDNTKIIYTETMTNPLLQV
                     SDIPALAKIAKKRGLKLIVDNTFTPMMVAPTQLGADITVYSLTKFVNGKNDCVAGAIC
                     ADQDFINSLIDVNNGTAMLLGPVLDPFRSSSILKNLHTLHIRMQQHSRNALYLAKRLT
                     EIGIKVNYPGLENHPGHNLLKSMMNPDFGFGGMLSFDAGSADKAAELMESMEMEGVGY
                     LAVSLGYFKTLFSNSGKSTSSEVPEDKQKEMGLAEGLIRFSVGLDNDIERTFAKMEKC
                     IRKAGLV"
     gene            complement(9650..>10106)
                     /locus_tag="PK345_06625"
     CDS             complement(9650..>10106)
                     /locus_tag="PK345_06625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34723.1"
                     /translation="KLKTFDIEKISESVQEPESEDDDDESGYGGRQGGGGSITSVRPM
                     ATVEEVVIAINLRFKNRISPEGVSVVENYLQALQKDNELKTTIKNNLTQNERQVYDLV
                     IKDIMDKLYKNYIINHSPQHYGELTQESVQGFINQSAYKMLREIIRTAA"
BASE COUNT         3032 a         1754 c         2173 g         3147 t
ORIGIN      
        1 gattcttgtc ggccatatca ctaaagatgg caatatagcc gggcctaaaa tccttgaaca
       61 tatggtggat actgttcttt attttgaggg cgatcgcaat tatggatacc gaattttgcg
      121 aactgtaaaa aacaggtttg gctctacaca ggagattggt atttatgaga tgtgcgataa
      181 tggattacga caggtttcaa acccctcgga gttgctcatt tcgcagcgca atgagaattt
      241 gagtgggatt gctattgcgt caacggttga agggttgcgg ccaatgctta tcgaaataca
      301 ggcattggta agtacagcag cctacggtgt gcctcaacgc gctacaacag gttttgaccc
      361 cagaagactt aacatgttac ttgccgtgct ggagaaaaga gcaggattca ggttagcaaa
      421 taaggacgtt tttttaaata ttgctggcgg aataaaagtg gacgatcctg ccattgacct
      481 tgctgtagta tgcgcagtac tctcttcaaa tgaggatatt ccgttgaatc agagggcgtg
      541 ctttacagcc gaagttgggc tctcgggaga aataagaagc gtgaacaggt tggatcagcg
      601 gattaatgag gcagctaaac ttggctttga aaaaatatat atcgccaagg gaggcttgaa
      661 aaaatctgat cccaagttga gacctgaaat agagataata ggcctgtcaa gtatcgaaga
      721 cctttttaga gagctttttg gataattatc attttagtat gctttcctga aaactaattt
      781 ttgggttacc attaattggt gaaagaactt gtaaggtgaa tcccgtgtta ttctatagaa
      841 gacttttttt tgtgagacta atccctattt agcgcttgtt tagtccaatc tcaagcgctg
      901 ttaatcctgg attcaaaagt tgattatcaa aagaggggtg ttttttatat gtgacaaata
      961 aaagtttaga tatatttgca atttgaaaca attctaaata gtatttaaaa aagttgagct
     1021 gtcgggattt atgaatttat gtgaacttaa agagggacag aaagggttca tttcaaaggt
     1081 taaagggcgt ggcgctttca gaaagaggat tattgaaatg ggctttgtga aggggcagga
     1141 ggttgaggtg gttaaaaatg ctccacttcg cgatcctatt gaatatcgaa ttatggatta
     1201 tttggtgtca cttcgcagga gcgaatccag gctcatacaa gttgtaagcg aacgtgaact
     1261 ggttgaagac gaaaatggcg ttgggtttaa cggtgtaatt gatactgaaa agcttaagaa
     1321 aaaaattagg gataaaggga aatcaattga tattgcgctt gttggaaacc ctaattcagg
     1381 gaaaacaacc atttttaatt tcgcttcaca ttcaagagag aaggtgggca attatggcgg
     1441 ggttacaata caatctaaaa cagctacatt caaacaaagt ggttatactt ttaacatagt
     1501 tgatttaccc ggtacctatt cattaacagc ttatactccg gaagaacttt tcgttcgtaa
     1561 tcatatcctt aagcaaaatc ctgatatcgt aattaatgtt gttgatgcgt caaacttaga
     1621 gcgaaatctt taccttacca cacagcttat tgatatggat attaaagtgg tagtggcgtt
     1681 gaatatgttt gacgaacttc aggctaaagg cgacaagttc gactatgcca tgcttggtaa
     1741 aatgcttggt attccttttg ttccaacaat aggatcgaag ggcaaaggaa ttcatgcact
     1801 gttcgcaaaa gcgattgagg tttatgagga taaagaccct ttagtaagac atatccatat
     1861 aaattacgga aaaccaattc aaaggtcgtt gttgaagata caagatgaat tatggaaaaa
     1921 taaatcgctt acagatattg tttcatcgcg tttttatgcg ataaaacttt tagaaaaaga
     1981 tgaaatcatt caaaaggagc ttgagtctct actcaatttt gaaattataa aaagggtagt
     2041 tgataatgag atcaggattc tggaaaatag ttttaaagag gactcggaga cgttgatcac
     2101 cgattccaga tatgggttta ttgctggcgc attaaaagag acttttactc aagataagct
     2161 taaacgtcag cagaagacag aaacagagat tattgatacc ttcatcacgc ataagatatt
     2221 tgggtttccc atctttctct ttttcctttg gttgatgttt tactcaacat tcaccttagg
     2281 tgaatatcca atgaaatgga ttgaagcggg tgttggtttt gtttctgagt ttgtcagcaa
     2341 atttatgcct gatggtatgt taaaagacct tcttgtagat ggagttatct caggtgtagg
     2401 cagtgttatt gtttttttac ctaacatcct catcttattc ttctttatta catttatgga
     2461 agatacagga tacatggcaa gggttgcctt tataatggat aggataatgc accggatagg
     2521 cttacacggc aggtcgttta tccctatgct catgggtttt ggctgtaacg ttccggcaat
     2581 tatggcaacc cgaacaattg agtcgcgctc cgataggata cttaccattt taataaatcc
     2641 ttttatgtcg tgcagcgctc gattaccggt ttatatttta attataggcg ctttctttcc
     2701 gtcaaacccc accatgatgc tattacttat ttatggtatt ggagtgatga ttgccgctat
     2761 tattgcaata gtctttaaga agatattctt tagaaaaaag gaggctcctt ttgttatgga
     2821 gttgcctccg tatcggctgc caacacttaa agcgatatta aaaaatatgt ggtataaggc
     2881 ctctttgtat cttcaaaaga tgggaggtat cattttattg gctgttatat tagtatgggc
     2941 tgccggatat tttcctcaaa acaggcaaca gatgtatgag tttgataaaa tgattggtga
     3001 tactaaacag gattttcaaa gtcaacttag tgaaattgac cctacaatag agactgaacg
     3061 ttttgaaagg ttacatgagg agatgaatat ggctattctt aaaatagata ttgataagca
     3121 aagttatcgt cttgagaatt catttattgg aaaatttggc cattttattg aacctttgat
     3181 acgtccgctt ggttttgatt ggaagatggg agtgagtttg gttactggag ctgctgcaaa
     3241 ggagattgtt gtaggtacgc ttggtgtgct ttatcatgct gatgacccag ggaatcatca
     3301 gtcgttgatt gagaagctga gaagtcagcg gcatcaatcc ggtccgaagg cagggcaact
     3361 ggtttttacg cccgttactg ctatggcttt tttgattttt gttttgatct attttccatg
     3421 tgtagccgtt gtggctgcgg taaagaagga gacgggcgga tggcgttggg cggcatttct
     3481 tgccctctac accacagtgc tggcttacct gctatcgctg ttggtatatc aggtagggat
     3541 tttaatttat taaaaagatg atccaggaaa tattaacata cataatactg ttggttactt
     3601 taaccttggt agtcaacaat gctatcaggt tttttaagga taattcggga ggatgtgccg
     3661 gctgcacgag tggggcaagt tgtaaaatag cggctttgaa acataaaatg gatgcggaaa
     3721 agtagttgga tcgctatttt ggtaacgtca atccagtctg tttaaaccct tgatagcaag
     3781 ttcataattg ggttttagtt caagggattt ctgatactct atccttgctt ccgacttgtt
     3841 gcctaagagt tcgtaacaat agcctctatt gtaataagcc tcataattgt taggatccag
     3901 ctcaacggct tgtgtgaaat aaccggctgc cgtttcatat tccttgaagt aaacaaggta
     3961 aatgtaacct agattatata aagcaaaggt aaattgtggt tcgatagcaa gtatgttatt
     4021 atatatctcg attgccttgt cgggttcatc gttttcctga aaaaacatcc caagtaagta
     4081 atatgcttcg ggcgaatttg gccttaattc tattgcattt gataaatagt ccgctgcaag
     4141 tggattacca aggttaatat acactacgcc aagttggata tatgcttcga aataatcttg
     4201 cttttgatct atggttgttt gtaaattcct gatcgcttta atggtatcac cttgttcaag
     4261 caaagcataa ccgcgtgtca ggtaagccgt cggattatag ctgtcgatct ttattagctc
     4321 atctattgtc tcgtacgttt tgtcgtattg tttcatgatc agaaacaatt gagccttttt
     4381 taataaagct tcctgatttt tcggttctat ctcaagtgct ttttcaaggc cttcgataca
     4441 gcggtcggga ttgcctaagg caagataggc ctccgagagg gtgatataat gattagggtc
     4501 ttgatcatta atatcaatag cgattgtaat atcgcgcaaa gcgctttgaa tagaatcaat
     4561 agtcagaaaa taattcgcac gttcgatata taacctatcg tcatttggat ttgaattaat
     4621 tttttcacta accctgagta tcaactgctc aacattcaaa ggttggtcag tctcacgcgc
     4681 agtatttttt tcactactat gacacgaaac tagaattacc aaagaaaata ataacccgga
     4741 aaaggtaaaa aatttcataa agagctaaaa tttgagcaga ttaatgatgt tgataaacag
     4801 ctaatttgaa gatttatttt atccaaaatt aatcaataat aagtcctttt gtctgaactg
     4861 ttgatttagc gtttttaaat ttcaatttat gttaatctga tttgttatat aaaacattaa
     4921 caatcaattt tttacaaaat ataaaaatcg cttttaagca atttgtccat tgtttactta
     4981 gcgaatgaaa acttttgtaa ggtgagcagc cctattatga aagttcgact ttttatgatg
     5041 ataatgctaa tgctatcctt ttgatagcct gaatgttaat aatatgtaaa gctattttgc
     5101 tgtttttttc aaaatctttt caattcattt tatataatta aatttgatga ctttttcaga
     5161 tagtcattgg gctttgttct cttttgattt tatcagaaaa gtttaaataa taacgggtaa
     5221 tagtaatgaa ggactttcat cacgcattaa agttagaggg tgagttgtgt atcggttgtt
     5281 caaaatgtat gaacatttgc cctacacaag ctatcagggt aaggaacggg aaagccatac
     5341 ttatgcccaa ccgatgtatt gattgtggtg agtgtttccg tgtttgccct gtcagcgcta
     5401 tcagtattga gcaggacgat gttgcccaaa tatttaatta taaataccgg atagccctga
     5461 tccctgccgt tttcactgct cagtttcctt cgaagtttag tattgccgac atttacaccg
     5521 ccttatatga gttagggttt actgacgttt ttgaggttga acatggagcg catattttac
     5581 gtgaagaggt aaaaaaaata attgacaatc ctgatttccc aaaaccatta atctccacct
     5641 tttgtcctgc tatagttagg ctgatacagg ttaaattccc ttcgctggtt cataatctga
     5701 tattgcttaa gcctccactc gatatttcag ctctttattt ccgtaggtct cttgccgaag
     5761 agggagtaaa agaggatgag attggaatat tctattttac gcagtgtgct gccaagatcg
     5821 cagccatcaa gagtccggct gaaagggtag aatccccaat aacaggggtg ataaatatgg
     5881 attctgcttt taataaggtt taccagtact tagaacaaaa tatccataat aaagaatcta
     5941 ttgcagaaag tcctgataat ttatcgccag aggctatttt atggagcctt acaacaggcg
     6001 aaacagagta ttatagcggg cgtcgaatag ccattgatgg tgtgcataat gtaattagct
     6061 ttttagaaaa gttggagaat gaggaaaacc caggtattca ttttttggag ctacgtgctt
     6121 gtcatgaggg gtgtgccggt ggcgtgcttg ctaatgaaaa tcgtttctta tctgccgaac
     6181 gaatgcgaaa ccgtgcaaaa gctgctgtaa acaatcaaga tggagatttc agtaacagga
     6241 tcaacgatta taaggacgac ctgaaagagt tgattaagac cgctgaggtt aagcctcgtt
     6301 cgatgcttaa acttgatgaa aacatgtcgg aagctatgcg taagatgaat cttatcaggc
     6361 agataataca gttgttacct atggttgact gcggcgcttg tggatcgcct gggtgttacg
     6421 cgctggccga agatatagtg caaggacgcg gtatgatgga aaattgcata tttatccaac
     6481 gtcgctacga gcagaaaggg atgcttaaaa atcaagatgc catagcgctg atgaagaaga
     6541 tatggggaga aaataaattt gaatcaaaat aaataacttt aagaaaaaat gactgtcaga
     6601 gaattagttg agaagcttgg gttgcatgtt tttagcgacc aatcgggttt ggaaaatgaa
     6661 ataaagagcg gttatacctc cgacttgctg agcgacgtaa tgggacatag caatcaaggg
     6721 gccgtatgga taacactgca aacccataag aacatcatgg ctatcgcatc attgaaagac
     6781 catgcagcaa taatcttggt aaaaggttta aaacctgatg atgacacatt ggcaacaagc
     6841 aatgaagaga agataccaat attaggtact gatcttcaga catttgaaat aacaggtaaa
     6901 gtgtaccagt taattaataa acagtaattt ccttattaat gcctgttttt agtgctgatc
     6961 tccatattca caccgtattg tcaccttgtg gtgatttgga tatgagccct gtaaatattg
     7021 taaaacgagc taaggagctt cagctcgaca ttataggcat tactgatcat aactcaaccc
     7081 gccattgccc gttgatacag caacttgcag caaaggaggg tatctttgtg ctttgcggcg
     7141 ccgaagtaac caccttggaa gaggctcact gtctttgctt tatgcccgac ctggaacggt
     7201 tagataagct ccaaagttac ctcgaccttt atctgatgga ttttatgaat gatgttaaca
     7261 aatttggcta tcaagtgcag gtagatgaag aggagacgat tgtatatgaa gagccaaaac
     7321 tgctgataca gtctatcaac cgtaacatta atcaggttga ggaatttaca cactctctgg
     7381 gagggctgtt catccccgca catattaatc gccctgcatt tagcctgata agccaattgg
     7441 gatttatacc ggatgatctt aactacgatg cactcgagat ttacaggatg acagatgaga
     7501 atttttgtaa tacttataac tatcggaaag atactacgtt gatcagaaat tcagatgcac
     7561 atcagctcaa cagaattggc gccgagagaa acttttttaa catagaagaa ttaaactttc
     7621 aggagattaa acttgcatta ttaaaacaac aggggaggtt tgtaaaatga ccgaaatttc
     7681 gcttcacatt ttggatatcg ttcataattc cattagggca aacgccaaaa agataaatat
     7741 ctctattaca gaagacctta aagaagataa atatcttctt aaaattgttg atgacgggaa
     7801 aggtatttct gctagtactc ttaaacatgt aaccgatcca ttcacgacat cgcgcaaaac
     7861 caggaaggta gggatgggcc tgccactgtt gaagcatagc gccgatcaaa ccggaggatg
     7921 gttgaaaatt tcttccgaaa taggcaaagg tacagaggta acatgctgct ttgggcttgc
     7981 ccatattgac cggccgccga caggaaatat tgcaagcatc atctatcagc tcctttcagc
     8041 atttcctgag ataaggtttg tatatacaca taaaacctct aacggagagt atctgtttga
     8101 taccgatgag ttaaaagaag ccctcgataa tttgcctgtt accatgccag aagtgagaaa
     8161 gtttattttg gaaatgattg aagagaattt aagagagata aaaatagcgc cataaaaatt
     8221 tagcattcca ctaattagca ttattgattg gcatattaaa ttgcaataat tgattttgct
     8281 tacctaagat ttgtaacttt gccttcagaa aatagtaatc aaaattttaa atttaatatt
     8341 atgaaattcg atccggctaa tagtattcag gatttactcc aattaggaga atatggagat
     8401 gttaatccct ccgtaacaga ttcggctacc tatacattta agcaagccaa aaccatgctt
     8461 gataccttta aaggcgaagc cgagggttgt tttctttatt cacgtcactg gaatccgagt
     8521 aataaatatc ttgctgatgc aatggcagct atggagggta cgcaagctgc gtgggtaact
     8581 gcgtcaggta tggcagcaat aacctgcgcc atcttgcaga tatgtagcgc cggcgaccat
     8641 attgtatcga gccggacaac ctacggaggt acttttgcct ttttcgaaaa ctacctcccc
     8701 aaactcaata tccaagttac aatggttgat gttaccgacc ttaaaaaggt agaagctgca
     8761 atacaggata acactaagat aatttatacc gaaacgatga caaacccgct tctgcaggtc
     8821 tctgatatcc ctgcattagc aaaaattgcc aaaaaaagag gtttgaagct aatcgtggat
     8881 aatacattta caccgatgat ggttgctccc acacagcttg gcgccgacat aactgtttat
     8941 agccttacca aatttgtgaa tggtaaaaac gactgtgtgg ctggcgccat ttgtgccgac
     9001 caggacttta tcaattcgct gatagatgtt aataacggaa cggcaatgct gttaggccct
     9061 gtgctcgatc cttttcgatc atcgtcaata ctgaaaaacc ttcatacact ccatatccgt
     9121 atgcagcagc atagccgcaa cgccctctat ttggccaaaa gattaaccga aattggtatt
     9181 aaagtaaact atcctgggct tgaaaatcat ccagggcata acctgctgaa aagcatgatg
     9241 aatcccgatt tcggctttgg cgggatgtta tcgtttgatg caggctctgc cgataaagct
     9301 gcagagctga tggagagtat ggagatggaa ggcgttggtt acctcgctgt aagcttgggg
     9361 tattttaaaa cgctgtttag caactcaggc aagagtacct catcggaagt tcctgaagat
     9421 aagcaaaaag agatgggttt agccgagggt ttgattcgct tttctgtagg attagataat
     9481 gatattgaaa gaacctttgc aaaaatggag aaatgtatca gaaaagctgg attagtataa
     9541 attcttgatg acactatacc aatataagtg tgtaagttta atatgaaggg ttcagtgttt
     9601 atggctccac gtatctaatt atgcttctgg tattgccata ttctatcttt tacgctgccg
     9661 ttctgattat ttctctcagc attttgtagg cgctttggtt tataaatcct tgaacgcttt
     9721 cctgtgtcaa ttctccgtag tgttgtggtg aatggttgat gatgtagttt ttatagagtt
     9781 tatccattat gtctttaatc accaaatcat aaacctgtct ttcgttttgg gtcaggttgt
     9841 ttttgatagt tgttttcagt tcattgtctt tttgaagggc ttgtaaatag ttttcaacta
     9901 ctgacactcc ttcgggtgaa atcctatttt tgaaacgaag attaatggca attaccactt
     9961 cttcaacggt tgccattggt cgaacactgg taatgcttcc gccgccgcct tgtctgccac
    10021 catatccact ttcatcatca tcgtcttcgc tttctggttc ctgaacactt tccgatattt
    10081 tctcaatgtc aaaggttttc agtttt
//
LOCUS       DAOOAI010000081        10031 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_8398_length_10031_cov_5.020449, whole genome shotgun sequence.
ACCESSION   DAOOAI010000081 DAOOAI010000000
VERSION     DAOOAI010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 10031)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10031)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10031
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8398_length_10031_cov_5.020449"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            17..1492
                     /gene="cysS"
                     /locus_tag="PK345_06630"
     CDS             17..1492
                     /gene="cysS"
                     /locus_tag="PK345_06630"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005633562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HPX34724.1"
                     /translation="MEHKIQLYNTLSRRKEIFEPLNPPHVGLYVCGPTVYGDAHLGHA
                     RPAITFDLVFRYMKYLGYKVRYVRNITDVGHLENDSDQGEDKVAKKARLEQLEPMEVV
                     QYYTLRYHKNMEQLNVLPPSIEPHASGHIIEQIELVKRILESGFAYEVNGSVYFDVVK
                     YDAEYNYGKLSGRSIEETLENTRELAGQEEKRNSVDFAIWKKASPEHIMRWPSPWSDG
                     FPGWHVECSAMGCKYLGDTFDIHGGGMDLIFPHHESEIAQSVAANGQDHVRYWMHNNM
                     ITIDGQKMGKSLNNFITLNQFFSGEHELLDQPYDPITIRFFILQAHYRSTLDFSNEAL
                     KAAEKGLKRLLKAADTLKTIKPSKSSTIEIKTWRNLCYQAMNDDFNSPIAIAHLFDAV
                     RMINSIDDGKETINADDLALLQTTYFDFVFTILGLKTDDKSQTDDHHLVEGLMKTILD
                     IRKEAKLKKDWATADKIRDDLGKLNIEVKDTKEGASWSKIE"
     gene            1690..2382
                     /locus_tag="PK345_06635"
     CDS             1690..2382
                     /locus_tag="PK345_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HPX34725.1"
                     /translation="MEKIVVLTGAGISAESGIRTFRDGDGLWKTHHFADLATPAAWRR
                     NPELVLEFYNDRRKQLLEVEPNAAHFALTKLEKKYDLHIVTQNVDDLHERAGSSKVLH
                     LHGELRKARSTINPDLIYNIDGWELKLGDKCEKGSQLRPHIVWFGEPVDDFEKAVEIV
                     SCADLLLVIGTSLVVYPAAGLLYHTKANIPIYLIDPGEPKFSQTSNLTFIQDKAASAV
                     PKLVEQLLDKGF"
     gene            2393..2848
                     /locus_tag="PK345_06640"
     CDS             2393..2848
                     /locus_tag="PK345_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014668.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory protein RecX"
                     /protein_id="HPX34726.1"
                     /translation="MHDPELKKIVLKAEKYCAAEERCKNDLLVKLANWGVEPEKAQTI
                     LEHLEDENFLSEKRFAKMYTTSKVNQNKWGKVRIELELMKRNIPEKYISEAINEIDEE
                     KYLKNFNVLVSFKSKESLDLNPLIRKQKIIAFLISKGYEEDYIQQNFHI"
     gene            2874..3969
                     /gene="prfB"
                     /locus_tag="PK345_06645"
     CDS             join(2874..2936,2938..3969)
                     /gene="prfB"
                     /locus_tag="PK345_06645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_118304733.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HPX34727.1"
                     /translation="MTLSENIKELEKRVAALRRYLDIDTKYEVISQKEEVTHQDNFWD
                     DSKKAEAILKELSEIRSWTEAYEEVKNACEDLSVILEFFNQSEATEEELNKAYTTTLG
                     LVEDLEFRNMLSDEEDRLSAVLNINSGAGGTESQDWAEMLMRMYIRWAELNKYNVKEL
                     DFQSGDTAGIKSVTLEIEGDFAYGYLKSENGVHRLVRLSPYDAANKRHTSFASVYAYP
                     LIDESIEIVINPADISWDTFRSSGPGGQNVNKVETAVRLRHAPTGIIVECQETRSQGL
                     NREKALRMLKSQLYELELRKRQEIQDEIEGNKTRINFGSQIRSYVMHPYKMVKDLRTG
                     FETSNVQAVMDGELEGFIKAYLMEFGQKNK"
     gene            3971..4720
                     /gene="kdsB"
                     /locus_tag="PK345_06650"
     CDS             3971..4720
                     /gene="kdsB"
                     /locus_tag="PK345_06650"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002560355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008690 - 3-deoxy-manno-octulosonate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HPX34728.1"
                     /translation="MKTIGIIPARYASTRFPGKPLVDIFGKSMIQRVYEQALKAETLD
                     HVIVATDDQQIFDHVNQFGGEVTMTSNSHNSGTSRCLEVVEKQFNYNFDLVINIQGDE
                     PFIDPQQINMVVKLFEDEKTEIATLVKKISKTEEIFNPNVVKAIIAKGNKVIYFSRSP
                     VPFFRGVPQDKWIQKGIFYKHIGIYGYRTEILKKINSLSESYLEKAESLEQLRWLENG
                     FSIFSTVSDFEGISIDTPEDLQKVIKTFRNK"
     gene            4765..5865
                     /locus_tag="PK345_06655"
     CDS             4765..5865
                     /locus_tag="PK345_06655"
                     /inference="COORDINATES: protein motif:HMM:NF036727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HPX34729.1"
                     /translation="MEIHKYISELLYDHDCVIIPGFGGFVANYHSARINTLQNSFHPP
                     YKKILFNKELKTNDGLLASHIAISEKIPYELVLKLIEDFSLEITNKLEAGEKIVLENI
                     GELYLDDEINIQFKQDYKINYLNDSFGLGVFISPPVQGNIKSSITISEKHQLPRKQPV
                     GVNKALQSAAYWSSGIAAAILFIGLIMLNFNSFEDFASSQTSLIPHFRIINKQNPSAE
                     KSKESASLKDQSIPNSQIDENITDAVIEDESLESNLTYEAPKTTSENLSWQPEAQPLA
                     ETEKEPLVVNTEVATQRMYYLIAGSFEKTENADNLINMFAKEGYTPKIIGQADNGFYR
                     VSIAAFFRKEDAIEELAKVREKYNPSIWLLRL"
     gene            5903..6577
                     /gene="trmB"
                     /locus_tag="PK345_06660"
     CDS             5903..6577
                     /gene="trmB"
                     /locus_tag="PK345_06660"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF001080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008176 - tRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HPX34730.1"
                     /translation="MTKRKLQHFAENATFPHFFQPDYKTLKTAFFLKGRWNKDFFKNN
                     APIVLELGCGKGEFTTGLASRYPDKNFIGIDIKGARMWRGAKTSFEASMTNVAYVRTQ
                     IELIEFVFGKQEVSEIWITFPDPFPQKPKSKKRLTSPQFLNRYRQIAKKNTIVHLKTD
                     NTSFFDYTLSTIVEQNLKLLSHTYNLDNNPVDSNAASIYTFYEEKFREKGESIKYLKF
                     RLFPEK"
     gene            6743..7843
                     /gene="ychF"
                     /locus_tag="PK345_06665"
     CDS             6743..7843
                     /gene="ychF"
                     /locus_tag="PK345_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007661265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HPX34731.1"
                     /translation="MGLKCGIIGITNVGKTVIFNCISKTKAETSNFAFSTSKSNIGVI
                     DVPDERLEKINSLIKSARIVPATFEIVDIPGLAKGASQGEGIGNKFLADIQQTDAIIH
                     VLRCFDDPELPHIEGSIDPVRDMEIVDLELQVRDLDLVQRKIQRLEKMAKSGDKEPKR
                     QVGILEKLVEHIENFQNVRDFELPEPDRSLIIGDMYLLTDKPIMYVCNVDEAYATTGN
                     KYSNNVIKALEGSNAHLLVIAGKLESEIAEFDDQAERMEFLTDAGLTEPGVNKMIRNA
                     FDMLDLQYFFTAGPKEVRAWTIRKGSTAHQSAGAIHSDMERGFIRAEVIKYEDLIHYG
                     SEHAVKEAGKFRVEGKNYIVQDGDILHIRFNV"
     gene            complement(7935..>10031)
                     /locus_tag="PK345_06670"
     CDS             complement(7935..>10031)
                     /locus_tag="PK345_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34732.1"
                     /translation="QVNIIPDQLTACHGKNQSFNGLVQATNYSSITWSTPDGGGSFFP
                     SPNVSQPLYIPDPEIDYPQGCIHFNVVVQPISPCVLSDHDEVTLCFQELPTVSLPDDT
                     TICFDDVLMLDATIANYSNFQWTTSGDGVFSDINDINGTYTPGVEDALIGEVEICLVA
                     NSISPCTSQVSNCMYITIQSTPSIVIPDQITICKDQFLNLFEYSEISNFDSLLWSTNG
                     DGVFIPSANIVDPIYQPGADDLENGCVELSVIAYPIEPCTNPVQNQFLLCFQQFPEVY
                     AGMNTTVCSTDQVLLIPEAFNNCGSYWETSGDGTFLTTDSLNYVYIPGEIDLLNGMVE
                     LCYFALPCAPCTDTAYDCLTVTFINLPSVNIIPDQSMVCYNENFSFEGLVDAENYNEI
                     IWGTTNGGGYFIFDNVLEPVYVPSPTVDYPQECIEIWVKVEPIGPCTITSTDNFSLCF
                     QPSPEVSLGSDMSLCQEDDIILTPNTQWGCSFSWETSGDGYFDNPSEMNVQYFPDQND
                     ISNGYVELCLTVTGCQPCTNTVTDCIMINIGKSQSISLLSGWSGISGYIEPFNNNIEL
                     VMTQALEALVIIYNFENEIFSPLLNINTLNAWDPYSGYVIKVNENAEISLCGTDIENP
                     TFQLNEGWNLLPVFSNTEVAINDLFAPFSENLIMIKEVAGTSLFYPQFDIFTLTTLSP
                     GKAYFVKVIQPFTITY"
BASE COUNT         3363 a         1684 c         2048 g         2936 t
ORIGIN      
        1 tgaaaattct tttaatatgg aacataaaat tcagctctac aatacattaa gccgccgtaa
       61 agagattttt gagccattaa atccgccaca tgtaggctta tatgtttgtg ggccaacagt
      121 ctatggagat gctcatctgg ggcatgccag acctgcaata acatttgatc ttgttttcag
      181 atatatgaag tatttgggtt acaaggtcag atatgttcgt aacataactg atgtcggaca
      241 ccttgaaaat gatagtgacc aaggtgagga taaagttgct aaaaaggcta gattagagca
      301 actggaacct atggaggtgg tgcaatatta cacactgagg tatcataaaa atatggagca
      361 gctcaatgtt ttaccaccaa gcattgagcc tcatgcatca ggccatatca ttgaacaaat
      421 agaacttgtg aagagaatct tagaatcggg ctttgcgtat gaggtcaatg gatcagtata
      481 tttcgatgtt gtaaagtatg atgctgagta caattatggg aagttatctg gtaggtctat
      541 tgaggagact ttggaaaaca ctcgtgagtt ggccggacag gaggagaaac gaaattccgt
      601 agattttgca atatggaaaa aggcttcgcc ggaacatatc atgcgttggc cgtcgccatg
      661 gagtgatggg ttcccgggct ggcatgttga atgttcggcg atgggttgta aatatcttgg
      721 cgacaccttc gatatacatg gaggagggat ggatcttata tttccgcatc atgagtctga
      781 gatagctcaa tcagtagctg ctaatgggca agatcatgta cgctattgga tgcataacaa
      841 tatgataacc attgacgggc aaaaaatggg aaaatcactg aacaacttca tcacattaaa
      901 tcagtttttc agcggtgagc atgaattgct tgatcaacct tacgatccta ttactattcg
      961 gtttttcatt ttacaggcac attatcgcag tacactcgat ttttcaaacg aggcgctcaa
     1021 agcagctgaa aaaggattga agcgtttgct gaaggctgcc gataccctta aaactattaa
     1081 gccgtcaaaa agttctacaa ttgagattaa aacatggcga aacctttgct atcaggcaat
     1141 gaatgatgat tttaacagcc caatagcaat tgcgcatctt tttgacgcag ttagaatgat
     1201 caactctatt gatgatggaa aagagactat aaatgctgac gaccttgcac tgcttcaaac
     1261 aacatatttc gattttgtgt ttacaatact tgggctaaaa accgatgata agagccaaac
     1321 agacgatcat catttagtcg aagggctaat gaaaaccata ctggatatcc gaaaagaggc
     1381 aaagcttaaa aaggactggg ctaccgcaga taaaataaga gatgatttgg gtaaacttaa
     1441 tattgaggta aaagatacca aagaaggggc ttcgtggagt aaaattgagt aatgtttgtt
     1501 tgaaaaggtg tgattccaat tgatgttaaa ttattaaaaa actgaagata ttaactcttg
     1561 ctaaatcaaa taataaacac ttgactgccg gttaggtaaa acgataagtc caattatttg
     1621 taattaaaaa agtgatagaa gataatttgt ttcgaataat ttgaaagtaa tttgtataaa
     1681 agaacagaga tggaaaaaat tgttgtgctc acaggtgcag gtattagtgc tgaaagcgga
     1741 atcagaacct ttagagatgg agatgggtta tggaaaactc accattttgc cgatctggct
     1801 acaccagccg catggaggag aaatcctgaa ttagtacttg agttttataa tgatcggcgt
     1861 aaacaactgc ttgaagttga acctaatgct gcacatttcg cattaacaaa gttggagaaa
     1921 aaatatgatc tgcacatagt aacgcaaaat gttgatgatc ttcacgaaag agctggttct
     1981 tcaaaggttt tacatttaca tggtgagctt cgcaaagccc gaagcaccat aaatcctgat
     2041 ttgatttata atatagatgg ctgggagctt aagctgggtg ataaatgcga aaagggtagt
     2101 cagttgcgcc cgcacattgt atggtttggc gagcctgtgg atgacttcga aaaagctgtg
     2161 gaaatagtta gctgcgctga tctcctttta gtaatcggaa cttctttagt ggtttatccg
     2221 gcagcaggac tattatacca caccaaagct aatattccta tttatttgat agaccctgga
     2281 gaaccgaaat ttagtcaaac atcaaatctc acttttatac aggacaaagc agcatcggcc
     2341 gttcctaagt tagttgaaca attgttggat aaaggatttt aatttgcttg aaatgcatga
     2401 tccggaatta aaaaaaatag tattaaaggc cgaaaagtac tgtgccgccg aagaacgctg
     2461 taaaaatgat ttgttggtga aacttgctaa ttggggagta gaaccggaaa aggcacaaac
     2521 gatattggaa catttagaag atgaaaattt tctttccgaa aagagattcg caaaaatgta
     2581 caccaccagt aaagtgaatc agaataaatg gggtaaagtg aggatcgaac ttgagcttat
     2641 gaaaagaaac attcctgaaa agtatattag cgaagcaatt aatgagattg atgaagaaaa
     2701 gtatttgaaa aatttcaacg ttttggtttc atttaaaagt aaagaatcac ttgatttaaa
     2761 tccattgata agaaaacaaa agataattgc gtttttaatt tccaaaggtt acgaggagga
     2821 ttatatccaa caaaattttc atatttaact aaacattaac aatagataat aaaatgactt
     2881 tatctgaaaa catcaaagag ttagaaaaac gggttgctgc tcttaggagg tatctttgac
     2941 attgacacca agtatgaagt aattagtcag aaagaggagg ttactcacca ggataatttt
     3001 tgggatgatt ctaaaaaggc tgaagccata ctgaaagaat taagcgagat tcgctcgtgg
     3061 actgaagctt atgaagaggt aaaaaatgct tgtgaagatc tttcggtgat tttagaattt
     3121 ttcaaccaat cggaagctac tgaagaggaa cttaacaagg cttatactac tacgcttggg
     3181 ctggttgaag atcttgaatt tcgaaatatg ttaagcgatg aagaagaccg gcttagtgct
     3241 gtgctaaata ttaattctgg agctggtggc accgaaagtc aagattgggc tgaaatgctg
     3301 atgaggatgt atatccgctg ggccgagtta aacaaataca atgttaaaga gttggatttt
     3361 caatcaggtg atactgccgg cataaagtcg gtaacactag agatagaagg agattttgca
     3421 tatggctatt taaaaagtga aaatggcgtc catcgattgg ttaggttatc gccttatgat
     3481 gctgcaaaca aacgtcatac ttcctttgcc tcagtttatg catatccttt aattgacgaa
     3541 tcaattgaaa ttgtcatcaa tccggcagat attagttggg atactttcag atcgagcggg
     3601 cctggcggcc aaaatgtaaa taaggtggag acagcagttc gattgcgtca tgcaccaact
     3661 gggataattg tagaatgtca ggaaacacgc tcccaagggc taaaccgcga gaaagccttg
     3721 cgtatgttaa aatcgcaatt gtatgaattg gaattgcgta agcgacagga aatacaagac
     3781 gaaatagagg gtaataaaac gagaatcaac tttgggtctc aaatacgttc gtatgtgatg
     3841 cacccatata aaatggtgaa agacctgcgt accggttttg aaacaagcaa tgtgcaagct
     3901 gttatggatg gtgaattaga gggcttcata aaggcttatc ttatggagtt cggacaaaaa
     3961 aataaataaa atgaaaacta ttggtataat tccggcaaga tatgcatcta caaggttccc
     4021 tggtaaaccg cttgttgaca tttttggaaa atcaatgata caaagggttt atgaacaggc
     4081 tttaaaagct gaaacacttg accatgttat cgttgcaaca gatgatcaac aaatctttga
     4141 tcatgtaaac cagtttggag gagaagtaac aatgacttct aactctcata atagtggaac
     4201 atcgcgatgc ctggaagtgg tagagaaaca atttaactat aattttgatc tggttattaa
     4261 tattcagggt gatgagccat ttattgaccc gcagcaaata aatatggtag taaaactatt
     4321 tgaagatgag aaaactgaaa tagctaccct tgtaaaaaag atttctaaaa ccgaagaaat
     4381 ctttaatccc aacgttgtaa aagcgattat tgcaaagggt aataaggtca tctattttag
     4441 ccgatcgccg gttccatttt ttcggggtgt gcctcaagac aaatggatac agaaagggat
     4501 tttttataaa catatcggaa tttatggtta cagaacagag attttgaaaa aaataaactc
     4561 actttctgaa tcttatcttg aaaaagctga atctcttgaa caactcaggt ggctcgaaaa
     4621 tggattttca attttctcaa cagtaagtga ttttgaggga attagtattg acacacctga
     4681 agatttgcaa aaagttatca aaacatttcg caataaatag tattttttta taccttagcc
     4741 catcaaaaaa aagcatttca agcaatggaa attcataaat acatcagcga gttgttatac
     4801 gatcacgatt gtgttatcat acccggcttc ggaggttttg ttgcaaatta ccactctgcc
     4861 cggataaaca cattgcaaaa ttcgtttcat cctccatata aaaagatact ttttaataaa
     4921 gagcttaaaa ctaatgacgg ccttctggca agtcatattg caatctctga aaagatacct
     4981 tacgaactgg ttttgaagct tattgaagat ttttctttgg aaattacaaa caaattagaa
     5041 gctggtgaaa agatagtttt ggaaaatata ggggaacttt atcttgatga tgaaatcaat
     5101 attcagttta aacaggatta taaaataaac tatctgaacg actcatttgg gcttggcgtt
     5161 ttcatttcac cgcctgttca gggaaatata aaaagctcga ttacgatatc tgaaaaacac
     5221 cagttgccac gcaaacaacc tgttggtgta aataaagcat tacaatcagc cgcctattgg
     5281 tcgtcaggta ttgcggcagc catactattt attggcttga taatgcttaa cttcaacagc
     5341 tttgaagatt ttgcaagtag tcaaacaagc cttatcccac atttcagaat aattaataaa
     5401 cagaatccca gcgctgaaaa atcaaaagag agcgcttctt taaaagatca atctattcct
     5461 aattctcaaa tagatgaaaa cattacagat gctgtaatag aagatgaatc tttagaatca
     5521 aatttgactt atgaagcccc gaaaaccaca tccgaaaatt tatcttggca acctgaagct
     5581 caaccattgg ctgaaactga gaaagaacct ttagttgtta atactgaagt agccactcaa
     5641 cggatgtatt atctcattgc aggatcattt gaaaaaaccg aaaatgctga taatctgatc
     5701 aatatgtttg caaaagaggg ttatactcct aaaatcatcg ggcaagccga taatggtttt
     5761 tatagagtga gtattgctgc ttttttccga aaagaggatg caatagaaga attggctaaa
     5821 gtgcgcgaaa aatacaaccc aagtatttgg ttattacgcc tttagtgatc cattgtcatt
     5881 tatccggaat tattttgtag atgtgaccaa gcgaaaattg caacactttg ccgaaaatgc
     5941 aacattcccg catttttttc agcctgatta taaaacttta aaaaccgctt tttttctaaa
     6001 aggaagatgg aataaagatt tttttaagaa caatgctcca attgttcttg agttagggtg
     6061 tggaaaaggg gagtttacta ccggattagc ttctcgttat cctgataaaa attttatcgg
     6121 cattgacata aagggcgccc gaatgtggag aggagcaaag acatcttttg aagcttcgat
     6181 gaccaatgta gcttatgtgc gtacacaaat tgagttgatt gaatttgtct tcggcaagca
     6241 agaggttagc gaaatatgga ttacttttcc tgatcccttc ccacagaaac caaaatcgaa
     6301 aaaacgactt acctcacctc aattcctaaa tcgttacagg cagatagcca aaaaaaatac
     6361 gatagtccat ttaaaaactg acaatacttc tttttttgat tataccctta gcactattgt
     6421 cgaacaaaac cttaaactac tctctcatac ttataattta gacaacaatc ctgttgatag
     6481 taacgccgct tccatttata ccttttacga agaaaaattt agggaaaagg gggagagcat
     6541 taaatattta aagtttagac tttttccgga gaagtagatt atttttggtt aaaacaagat
     6601 tttaaaaagt aaaaatcgtt gaccaaggag gtaattctta atattgttct tgattatttt
     6661 atgttttcat caggttttag cctttgtact aagtttacat ttatattaat tttgcttttt
     6721 aaaacatgaa aaaccaaata taatgggact taagtgtggc attattggta ttaccaatgt
     6781 tggtaaaacg gtaattttta attgcatatc taagactaaa gctgagactt caaattttgc
     6841 ttttagtacc agtaaatcca atatcggtgt aattgatgtg cctgatgaac ggctcgaaaa
     6901 gattaattcc ttgattaaat cagcgcgtat tgtgccggca acctttgaaa ttgttgatat
     6961 accaggcctt gcaaaaggcg ctagtcaggg tgaaggtatt ggaaataaat tcctggccga
     7021 tatacaacag actgatgcca taattcatgt attacgctgt tttgatgatc cggagctgcc
     7081 tcatatcgaa ggttcaattg atccggtgag ggatatggaa attgttgatc tggaattgca
     7141 ggttcgcgat cttgaccttg ttcagcgtaa aatccaaaga ttggaaaaga tggcaaaatc
     7201 gggagataaa gagcctaaaa ggcaggtagg tattcttgaa aagttggttg agcacatcga
     7261 aaattttcaa aatgttcgtg attttgaact tcctgaaccc gacagatcac taataattgg
     7321 cgacatgtat ttgcttactg ataaaccgat aatgtatgtg tgtaatgttg acgaagctta
     7381 tgcaactaca ggtaataagt attctaataa tgttattaaa gcccttgaag gctcaaatgc
     7441 ccacttgtta gtgattgccg gaaagttgga gtcggaaatt gccgaatttg acgatcaggc
     7501 cgaacgaatg gagtttctta ctgatgccgg attaactgag ccgggagtaa ataaaatgat
     7561 ccgaaatgct tttgatatgc ttgatttgca atattttttt actgccgggc caaaagaggt
     7621 tcgagcctgg acaattcgca aaggctctac agctcatcaa tcggcgggcg ctattcatag
     7681 cgatatggaa cgcggtttta ttcgtgctga ggttattaaa tatgaagatc tgatccatta
     7741 cggttcggaa catgccgtta aagaggcagg aaaatttcgc gttgaaggta aaaactacat
     7801 cgtacaggat ggagatattt tacatatacg gtttaatgtg taaaaagatg cagaattgta
     7861 ttttaaaaaa ttacaagcct gtttttgttt tgcattaacc tttccttact atatattgct
     7921 gcattacaac aatatcaata ggttattgta aacggttgga taacttttac aaagtaagct
     7981 ttgcctgggc ttagggttgt taaagtaaaa atatcgaatt gaggataaaa tagacttgta
     8041 cctgccacct ctttaatcat tatcagattt tcggaaaatg gcgcaaaaag atcattgata
     8101 gccacttcgg tattactaaa taccgggagt aagttccaac cctcattaag ctgaaatgtt
     8161 gggttctcaa tatcggtccc gcacaaactg atctccgcat tctcatttac ctttattaca
     8221 tatcccgaat agggatccca tgcattgagt gtgttaatat ttaataatgg gctaaatatt
     8281 tcattttcaa aattataaat gattacaagc gcttcaagag cttgtgtcat cacaagttcg
     8341 atattgttat taaagggttc gatatatcct gaaattccac tccatccaga aagcaggcta
     8401 atagactgag attttccaat gtttatcata atgcaatcag taacagtatt tgtgcagggc
     8461 tggcatccgg ttactgtgag acatagctct acatacccat ttgaaatatc attttgatca
     8521 gggaaatatt ggacattcat ttccgatgga ttatcaaaat agccatcgcc ggaggtttcc
     8581 caagaaaagc tacaacccca ttgcgtattt ggtgtaagta ttatatcgtc ttcctgacag
     8641 agcgacatat cactacctaa agatacttcg ggagagggct ggaaacataa agaaaaatta
     8701 tcggtactgg tgatagtaca aggaccaatt ggttctactt taacccaaat ctcaatacat
     8761 tcttgtggat agtcaacagt agggcttgga acataaaccg gctctaaaac attgtcaaat
     8821 ataaaatatc ctccgccatt tgtagtcccc caaattattt cattgtaatt ttcagcatcc
     8881 actaatcctt cgaaactgaa attttcgtta tagcatacca tggattgatc tggaataatg
     8941 tttactgatg gtaaatttat aaaggttaca gtaaggcagt cataggctgt atcggtacat
     9001 ggtgcgcaag gtagtgcgaa atagcataat tctaccatac catttaaaag atctatttcg
     9061 cccggaatat aaacatagtt gaggctatca gttgtcagaa aagtaccatc gccagatgtt
     9121 tcccagtaac tgccgcaatt gttaaaggct tcaggaatta acaaaacctg atcagttgag
     9181 caaactgtcg tattcatgcc agcataaact tcaggaaatt gctgaaagca taataaaaat
     9241 tgattttgta cagggtttgt gcaaggttca ataggataag caattaccga aagttcaaca
     9301 catccatttt ccagatcatc agcccctggt tgataaatag gatcaacaat atttgcagaa
     9361 ggaataaaaa ctccgtcacc attagtactc caaagcaacg aatcgaaatt tgatatctcc
     9421 gaatattcaa ataggttcag gaactgatct ttgcaaattg ttatctgatc gggtataaca
     9481 atacttggag tgctttgaat tgtaatatac atgcaatttg aaacctgact tgtacaagga
     9541 gaaattgaat tggcgactaa acagatttcc acttcaccta tcaaagcatc ttcaactccc
     9601 ggcgtgtaag ttccattaat atcatttatg tcgctgaaaa ccccatcgcc actcgtagtc
     9661 cattgaaagt tggaataatt ggcaatagta gcgtcgagca tcaatacatc atcaaaacag
     9721 atagtggtat catctggtaa actcactgta ggcagttctt gaaaacatag tgtaacttcg
     9781 tcatgatcgc ttaatacaca aggacttatg ggttgtacaa ctacgttgaa atgaatacaa
     9841 ccttgaggat agtcaatttc aggatcggga atatacaaag gttgtgatac atttgggctg
     9901 gggaaaaagc ttccgcctcc atcgggtgtt gaccatgtaa tagaggagta attagtggcc
     9961 tgaacaagcc cattaaaact ttgattttta ccatgacaag cagttagctg atcgggaatg
    10021 atgttgactt g
//
LOCUS       DAOOAI010000082         9779 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_8662_length_9779_cov_4.236425, whole genome shotgun sequence.
ACCESSION   DAOOAI010000082 DAOOAI010000000
VERSION     DAOOAI010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9779)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9779)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9779
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8662_length_9779_cov_4.236425"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(388..540)
                     /locus_tag="PK345_06675"
     CDS             complement(388..540)
                     /locus_tag="PK345_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34733.1"
                     /translation="MQTLKTDYKENFISVNKKSKLYNTFYAYPTLNHDWPNKINSSVK
                     NALSIA"
     gene            652..1494
                     /locus_tag="PK345_06680"
     CDS             652..1494
                     /locus_tag="PK345_06680"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPX34734.1"
                     /translation="MKSIFSISELEIKARELRVDVIRSLTSAGSGHLGGSLGLADVFA
                     VLYFHELKHNPAEPLWSERDRLVLSIGHVAPILYAALANSGYFDREELLTLRKLGSRL
                     QGHPGRDHGLPGIELSAGSLGQGLSVAVGMALADKMDKNRRRVYVVLGDGELQEGSVW
                     EAAMSASHYNLDNLIALVDRNRVQIDGRVKNVMEIEPLADKWKAFGWYVIECDGNSID
                     ELIHAFEKARSIVEKPVVILANTLMGKGIPEIENDYRWHGKVPTQAEAVRFINTLLSD
                     GITN"
     gene            1505..2098
                     /locus_tag="PK345_06685"
     CDS             1505..2098
                     /locus_tag="PK345_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34735.1"
                     /translation="MKRKGIYLLSIALFVMSSCDLLQQAEGARMLSNCEFKIQNLSDV
                     RLAGVDVSGISNVSEINTLDVLAITNALLSSNLPLTFNLNLLVKNPNQQPASMNRLDW
                     ILFIDDMQMLDGSVNERFVAEPQGVGLLPVKINFNLSEVLKGETKDKIINTAIGLVDG
                     SGQSARVMIKLKPSIMVGQHTLMYPKWLEVKHEFTAK"
     gene            2101..3051
                     /locus_tag="PK345_06690"
     CDS             2101..3051
                     /locus_tag="PK345_06690"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HPX34736.1"
                     /translation="MRLKRSDVPTRIGFGQGVLAAAQKNSNVVALGADITSSVSLDFF
                     KAAFPDRFFSLGIAEQNCVGVAAGLALSGKIPVFSTYGVFSAMRPADQIRISVCYNDV
                     HVIIGGAHAGISVGPDGATHQALEDIAIVRVLPNMTLISPCDATQARLITEKAILNLK
                     GPVYIRYGREAVPDFTDESQEIEIGKGELMRNGTDITIISTGHLTWEALEAASILEKE
                     NISARVINIHTIKPLDKDIIVKAACETGAIITAEEHQIFGGFGSAVAEVVVNHCPVPM
                     EFIGIGDSFGESGKPDELMEKYGLKASSIVNKAMGLLLRK"
     gene            complement(3091..5388)
                     /locus_tag="PK345_06695"
     CDS             complement(3091..5388)
                     /locus_tag="PK345_06695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34737.1"
                     /translation="MQSFTKVERAFSSKIERRHPVNIPYIIVENYPKLGLLTALRFIE
                     WVNENPYGVISLPTGKTPESFIFWAKKILSEWDTTGGKKLREENGLLISKKPNFNNLH
                     FVQIDEYYPVGSQQHNSFYHFVEKNYIENFGLDKSKGQFINCDMIALAEGRHFSEIFP
                     NGIVDLNLRFREAKTSKEKLQQESIFILDEWCSNYEQKVRDLGGIGFFLGGIGPDGHI
                     AFNTRGSDHHSTTRLTRANYETQAAAAGDLGGIEISKNRLIITIGLQTITFNPEATAI
                     IFAAGEGKAPMVANALEEAPSNLNPASALAKLKNARFYITHGAASMLKDYKYHYFAST
                     PWNQQKTDRAVMDLCEKINKFGEHLTIDDLKKDEYCSMIPNLDHHTVQNVINSVKQKI
                     ERGTFTFSKQRILYTGPNHDDIILGLLPHIHRLSRNETNRSHFAVMTSGADTVTNKFL
                     IKSLEDTLNFLNADQIQMVNYPDFFEKGYKYKRDKDIYHYLIKVASGQPEERRRGFSH
                     RMVRNIVEIWSVESIQQLRDKIEDILSITRMSYGGQKLPPQVQALKGMIREFEEELVW
                     AFFGMTSNEVHHLHLTFNVDNAANYKQEITTILSKLQQIQPTVISVVLNPEGSGPNVY
                     YKSLQAIAAALIEWGKTKDLSEVRIWGYRNILHKFHPAEVTHIVPVSLNAMGVLNESF
                     TNSYLSQVSASFPNYKLDGKLSDLSKQIWVEQLKSVQFLLGKAFFYQNESPRLRTTHG
                     LLFFNEMNVDEFLLKVKDLKLFIEG"
     gene            complement(5583..5981)
                     /locus_tag="PK345_06700"
     CDS             complement(5583..5981)
                     /locus_tag="PK345_06700"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HPX34738.1"
                     /translation="MEMKIFFEGKKKVTAEINGQIIPTDQPVQSGGDGSAPAPFTLFL
                     ASIGTCAGIYIKSFCDQRNIPTDEIYITQKMNYNYKTRLIDHIELKVNLPASFPEKYK
                     PALINAANLCAVKEHFKSPPEIEVNTYTFD"
     gene            complement(6103..>7784)
                     /locus_tag="PK345_06705"
     CDS             complement(6103..>7784)
                     /locus_tag="PK345_06705"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HPX34739.1"
                     /translation="IETTDIHGRIFPYDLINDKPLDHSLAHVADFVRQERENKDQEVI
                     LLDNGDILQGDPIIYFNNFEKKESKHLVSMAMNEMQYDAGALGNHDIEPGPEVYFKLK
                     EEFNFPWMAANAVDQASGEPVFQPYTIIHRKGGKVAVLGLITPAIPKWLPEHTWEGIV
                     FQDMIEAAAKWVALIKEKDKPDLMVGLFHSGVDYTYGNQNENTPCNENASQLIAERVE
                     GFDIIFVGHDHQGWNKIVNSPDGNEVLLLGAKNDARNVAVATINLTMNKKNRRYEKDI
                     SGELINMNEYNADQSFMTKFTPSFHEAQQWADFKVTELGGRLVASQSLFGDAAFTDII
                     HQLQLSLTDADISFTSPQTFNLTIEPGRLTVKDMFKFYRYENQLYTITLTGKEIKDFL
                     EYSASLWFNKMDSSDDNLLLFKMDADGKVVKNNQNKALLANPYWNFDSAEGISYTVNV
                     DKPEGERVIINQFNDGRPFDLTKNYSVALNSYRGSGGGDHLTIGCDMTKEEIKDRIIF
                     KSEKDIRWHLTDWLKQQDTFIPAANSNWKITPVEWVKEAKIKDSILLFGSR"
     assembly_gap    7785..7794
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(8022..9692)
                     /locus_tag="PK345_06710"
     CDS             complement(8022..9692)
                     /locus_tag="PK345_06710"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HPX34740.1"
                     /translation="MKTDFELAKEAKMLHINAIAEKLNINPDDLDLYGRYKAKIPLEY
                     INEERAKKSKLILVTAMTPTPAGEGKTTVAIALAQALNKIGKNAVVVIREPSLGPVFG
                     IKGGAAGGGMSQVLPMEDINLHFTGDIAAIEKAHNLLAALIDNNIQSKKNNLNIDPRS
                     ITWKRVIDMNDRALRKVIIGLGGQSSGIPRQSGFDIAVASEIMAILCLVANIEELKCK
                     LGSIYIGCKYDKEPVFARDLKAHGAMAVLLRHAIKPNLVQTMENTPAIIHGGPFANIA
                     QGTNSVIGTKMALSLSDYVITEAGFASDLGAEKFFNIKARYGGLTPNLAIIVATVRAL
                     KFHGGVKRKELNNPNPNAVFNGLENLDKHIENIQHFNVNPIVTINQFTSDTEEELNII
                     INHCKNKGVNVAINNGWAKGGEGSLELAELVISEAVKSNDAFRFLYELESPIREKIKI
                     IAQKMYGASNVEYSNSANKIIQRIKKLGFSNLAICIAKTQNSLSDNSSLIGRPTDFTV
                     SIRDIELATGAGFIIPIAGEIMRMPGLPATPAAENIDIDLKGNITGLF"
BASE COUNT         2671 a         1858 c         2063 g         3177 t
ORIGIN      
        1 taggtaagat gggtaaaaag aagaaatctt tgcctaagga gggaataact ttcggtaaaa
       61 agagggcttg aaaagtattt gtttcgtctc atagttccct cctcttaggg gtttgcagcc
      121 tatggccgaa gtgtttttac gtatggggct atattgaaaa tttagctttc tcacctcacg
      181 taagtttttt ttatttaaaa aataataaaa atcaactaca aagataataa aatatcttag
      241 atatgtatgt tattttttgt taaaaatgat aatacttatc atatcatcta ctttacattt
      301 tacatccatc tattgtaata ttactttaca ctgcccgact gcgtcgccgt tgtggagcca
      361 aagaccatag agccgaattt tacttagtta ggcaatagaa agcgcatttt tgaccgaact
      421 gttgatttta ttaggccaat catggttaag tgttggataa gcataaaacg tattgtaaag
      481 tttgctcttt ttgttaacgc tgataaagtt ctctttgtaa tcggttttaa gagtttgcat
      541 ttttttagtt taatctaaac acgctcagta ctatgattaa taagtcatta ttatgtattg
      601 tccatatttg aaaattgttt attttcgccg atattctttt taattaatag gatgaagtct
      661 attttttcga tcagtgaatt agagatcaaa gctcgtgagc ttcgggttga tgttatcaga
      721 agccttactt ctgccggcag tggtcattta gggggcagtc ttggtcttgc agatgttttt
      781 gcggtactct attttcatga gctgaaacat aatcctgccg agcctttatg gtctgagcgc
      841 gaccgtttgg ttttatccat aggtcatgtt gctccaatac tttatgctgc gcttgccaat
      901 tcgggttatt tcgatagaga ggagttgctt actcttcgta agcttgggtc gaggttgcag
      961 gggcatcccg ggcgcgatca cgggctgccg ggcattgagc tgtcggccgg ctcgctgggt
     1021 caaggattat cggtagctgt tggtatggca ttagccgata agatggataa aaataggcgt
     1081 agagtttatg ttgttttagg cgatggcgaa ttgcaggagg gatcggtatg ggaagccgcg
     1141 atgagcgcat cgcattataa tttggataac ttgattgccc tggttgaccg aaaccgtgtt
     1201 cagattgacg gtcgagtgaa aaatgtgatg gagatagaac cgcttgctga taaatggaaa
     1261 gcttttggct ggtatgttat cgaatgtgat ggaaactcaa ttgacgagct gatccatgcc
     1321 ttcgaaaagg caagatccat agtcgagaag cctgttgtaa ttcttgcaaa tacactgatg
     1381 ggtaaaggaa taccggagat agagaatgac taccgttggc atggaaaagt tcccacgcag
     1441 gctgaagctg ttagatttat caacacacta ttgtccgacg gcataactaa ctaatttggc
     1501 gtatatgaag cgaaaaggta tttatttgtt atcaatagcg ctgtttgtaa tgtcgtcctg
     1561 tgatttatta cagcaggcag agggcgctcg tatgctttcc aattgtgagt ttaaaatcca
     1621 aaatctttcc gacgtcaggc ttgccggcgt ggatgtttcg gggataagca atgtgtcgga
     1681 gataaataca ttagatgtat tagcaataac aaatgcttta ttaagcagca acttgccact
     1741 gacgtttaac cttaaccttt tggtaaaaaa tccaaaccaa cagccggctt cgatgaaccg
     1801 cctcgattgg atccttttta ttgatgatat gcaaatgctc gatgggtccg ttaatgagag
     1861 atttgttgct gagccgcaag gtgtcggcct tttgcctgta aagataaatt tcaacctctc
     1921 agaggtgttg aaaggcgaaa cgaaagataa gattatcaat actgctatcg ggcttgtgga
     1981 cgggtctggt cagtcggcga gggtaatgat taagctaaaa ccgtcaataa tggtcggtca
     2041 gcatacattg atgtatccta aatggcttga ggttaaacac gaatttactg caaaataaat
     2101 atgagactaa aaagatcaga tgttcctaca aggattggct ttggccaagg tgtgctggct
     2161 gcggctcaaa agaatagtaa tgttgttgct cttggcgctg acatcacctc ttccgtcagt
     2221 cttgattttt ttaaggcggc ctttcctgat agattttttt cattaggtat tgccgagcaa
     2281 aactgtgttg gtgtggctgc aggattggca ttgtcgggta aaataccggt attctctact
     2341 tatggtgttt tttcggccat gcgtcctgcc gatcagataa ggatatcggt atgttataac
     2401 gatgttcatg tgattatagg aggcgcccat gccggcattt cagtaggccc tgatggggct
     2461 actcatcagg ctttagagga tattgccatc gttagagttc ttccaaatat gactctcatc
     2521 tccccatgcg atgctacaca ggcgcgcctt attaccgaga aggcgatttt aaatttaaaa
     2581 ggccctgttt acatacgtta tggacgcgag gcagtacccg attttactga tgaaagtcag
     2641 gaaatagaga ttggaaaagg agagcttatg agaaacggta cagatattac tatcatctct
     2701 accgggcacc tgacatggga ggctttggag gctgcttcta ttcttgaaaa agaaaacata
     2761 agcgccagag tgatcaatat tcacacaata aaacctttgg ataaagatat aattgtgaaa
     2821 gctgcctgcg aaacaggcgc cataataact gccgaagagc atcagatttt tggtggtttc
     2881 ggaagcgccg tggccgaagt agtagtgaat cattgcccag ttccaatgga atttatcgga
     2941 ataggcgata gcttcggcga atcgggcaaa cctgacgaac ttatggaaaa atacgggctt
     3001 aaagcatcca gtattgtaaa taaagcaatg gggcttttat taagaaaata acggttgcta
     3061 cggattgata agatataatt atttacaggt ttagccttcg ataaataatt ttagatcttt
     3121 tacctttaaa agaaactcat ctacgttcat ttcgttaaaa aacaataatc catgggtagt
     3181 gcgcagtcga gggctttcat tttgatagaa gaaagctttt cctaacaaaa actgaacgct
     3241 ttttaactgt tcgacccata tctgctttga gagatcactt aacttaccat cgagcttgta
     3301 attgggaaat gatgcactaa cctgacttag gtaactattt gtgaacgatt cgttcaaaac
     3361 tcccattgcg ttgagcgata cagggacgat atgtgttacc tcggcgggat gaaacttatg
     3421 caggatattt cggtagcccc agattcgcac ttcgctaagg tcttttgtct taccccattc
     3481 gattagcgct gcggcaatag cctgtaacga tttgtaatag acattcggcc cactaccttc
     3541 cggattaagt acaacgctta taaccgtagg ttggatttgt tgcaacttcg ataagattgt
     3601 cgtaatctct tgcttgtagt ttgctgcgtt atccacgtta aaagtaagat gtaagtggtg
     3661 gacttcatta ctggtcatgc caaaaaatgc ccaaacgagc tcttcttcga attccctgat
     3721 catcccttta agagcttgta cttgcggtgg tagtttctga ccgccatagc tcattcgagt
     3781 gatcgaaaga atgtcctcta ttttatcgcg gagctgttgt atgctttcca ccgaccatat
     3841 ttcaacaatg tttctaacca tcctgtgcga gaatcctcgt cttcgctctt ctggctgccc
     3901 tgaagccact ttgatcaggt agtgatatat atctttatcc cttttatatt tatatccttt
     3961 ttcgaaaaag tcggggtaat taaccatctg tatttggtcg gcgttgagga agttaagagt
     4021 atcttcaagg gatttgatca aaaatttgtt ggtaacggta tcggctccgg aggtcatcac
     4081 agcgaaatgc gaacggttgg tttcgttacg cgaaagcctg tgaatatgag gcagcagtcc
     4141 aagtattata tcgtcatggt tcggtccggt atataaaatt ctttgtttgc taaaagtaaa
     4201 agtgccacgc tcaatctttt gtttaacgga gtttataaca ttttgtacgg tatgatggtc
     4261 gaggttgggg atcatgctgc aatattcatc ttttttgagg tcgtcaatgg taaggtgttc
     4321 tccaaatttg ttgatcttct cgcaaaggtc cattacagct ctgtctgttt tctgttggtt
     4381 ccaaggtgta gatgcaaaat aatggtactt gtaatctttt aacatactgg cagcgccatg
     4441 ggtaatgtag aagcgtgcgt ttttaagctt tgcaagcgct gatgcggggt ttaggtttga
     4501 tggagcctcc tctaaggcgt tagctaccat tggagccttc ccttcaccgg cggcaaaaat
     4561 tatggctgtt gcttcagggt taaaggttat agtctgtagt ccgatagtta ttataagtct
     4621 gtttttggat atctctatcc cgcctaagtc gccggcagcg gcagcctgag tttcatagtt
     4681 tgcacgagta agccgtgtag tggaatgatg atcggagcct cgggtattaa aggcaatatg
     4741 accgtcgggg ccaataccgc caagaaaaaa tccaatacct cccaaatctc ttactttttg
     4801 ctcataatta gaacaccact catcaagaat aaaaattgat tcttgctgta gcttttcttt
     4861 cgatgttttt gcttcccgaa agcgtaaatt taggtcaaca ataccatttg gaaagatttc
     4921 gctaaagtgc ctaccctctg caagcgctat catatcgcaa ttgataaact gacctttgga
     4981 tttatcgagc ccaaagtttt cgatatagtt tttttcgacg aaatgataaa agctattgtg
     5041 ttgctgggac ccaacaggat aatactcatc tatctgaaca aaatgcaaat tgttgaagtt
     5101 gggttttttg gatatcagca atccattttc ctctcttaat ttttttcctc cagtcgtatc
     5161 ccattcactt aggatttttt tagcccaaaa aataaaactc tcaggtgttt ttcctgtcgg
     5221 aaggctaatt acaccatacg gattttcgtt tacccattca ataaaacgca gtgcggtaag
     5281 aaggccgagt tttgggtagt tctctacaat gatgtagggg atatttacag gatgtcgtct
     5341 ctctatttta gaagaaaagg cacgttcaac ttttgtaaaa ctttgcatat tcatctgact
     5401 ttattaaggt taaggttatt cgtagtgtga tccatcaata gaataacttt tgatttatct
     5461 gaagcaaaaa taagtcaaaa aaattagttg gttgttggtt tttggttagt tgatgttatt
     5521 tgagcttagg tctgtcattg ttttaaaata aaaacctccg ttgttggcgg aggtttttca
     5581 aattaatcaa acgtatatgt gttgacttca atttcgggtg gcgacttgaa gtgttctttt
     5641 acagcgcaaa gatttgcggc atttatcagg gctggtttgt acttttcggg gaaagaggcc
     5701 ggtaggttaa cctttagctc aatgtgatca ataagccgtg ttttataatt atagttcatc
     5761 ttttgtgtta tataaatttc gtctgtaggt atgtttcgtt ggtcacaaaa agatttaata
     5821 taaatccctg cacaagtgcc gattgatgct aaaaaaagtg taaaaggggc aggggcgcta
     5881 ccgtcgccac cggattgcac aggctgatcg gtaggtatga tctgaccatt gatctcagca
     5941 gttacctttt tcttgccttc gaaaaagatt ttcatttcca taaaacgtag aatattaacg
     6001 attcgataag gcaaaaataa aacatcattg ttgattagat gtgtgctgta agatattgtt
     6061 aaaagcttac agattcagag ttttagaaaa atgatccggt aattatcttg atccgaaaag
     6121 caaaatacta tcttttattt tggcctcttt cacccattca accggggtta ttttccagtt
     6181 actatttgcg gccggtatga acgtatcctg ttgttttagc caatcggtaa gatgccatct
     6241 gatgtctttt tccgatttga aaattatccg atcttttatc tcttcttttg tcatatcgca
     6301 accgatagta agatgatcgc cgccgccact gccacgatag gaattaaggg ctaccgagta
     6361 attttttgtt aagtcaaatg gccttccatc attaaactga tttataatga ctctctctcc
     6421 ttcaggttta tccacattta cagtgtaact tatgccttca gcggaatcaa aattccaata
     6481 gggattggct aataaagctt tgttttgatt gtttttcaca accttgccgt cagcatccat
     6541 tttgaataga agtaaattat cgtcgctgct atccatttta ttgaaccaaa ggcttgccga
     6601 atattcaaga aagtccttga tctctttacc tgtgagggtg atggtataga gctggttttc
     6661 gtaacgataa aatttaaaca tatcttttac agtaagcctt cccggctcaa tagtgaggtt
     6721 aaaggtctgc ggcgaggtaa atgagatgtc ggcatcggtt aggctaagtt gaagttgatg
     6781 tataatatcg gtaaaagctg catcgccaaa caaagattgt gaagccacta atctgccgcc
     6841 taactcggta accttaaagt cagcccattg ttgtgcttca tgaaatgatg gggtgaattt
     6901 tgtcataaaa ctttgatcgg cattgtactc gttcatattt atcagctcgc ccgaaatatc
     6961 tttttcgtat ctcctgtttt ttttgttcat cgtgagattg attgtagcaa cagctacatt
     7021 acgggcatcg tttttagcgc ccaatagcaa aacttcattg ccatcgggtg aattgacgat
     7081 tttattccat ccttgatgat cgtgccctac aaaaattata tcgaatccct ctacacgttc
     7141 ggctataagt tgtgaagcat tttcattaca aggcgtattt tcattttgat tgccataagt
     7201 ataatccaca cccgagtgaa acaatccgac cattaggtca ggtttatctt tttccttgat
     7261 caatgcaacc cattttgcgg ctgcttcgat catatcctga aatacgatac cttcccacgt
     7321 atgttcaggt agccattttg gaatggcagg cgttatcagt ccaagaactg ctacttttcc
     7381 tcctttacga tggattattg tataaggctg aaaaaccggt tctccgcttg cctgatcaac
     7441 agcgttggca gccatccacg ggaaattaaa ttcttcttta agtttaaaat aaacctctgg
     7501 ccccggttca atatcatggt tgcccaaagc tccggcatcg tattgcatct cattcatcgc
     7561 cattgatact aagtgtttgc tctctttttt ttcgaaatta ttaaaataga taatcggatc
     7621 gccttgcaga atatccccat tgtcgagcag gatgacctcc tgatctttat tttccctttc
     7681 ctgccttaca aagtcggcta catgtgccaa agagtgatcc aaaggcttgt cgtttatgag
     7741 atcgtatgga aaaatacgtc catggatgtc ggtggtttca ataannnnnn nnnnggaaac
     7801 atacggaatt cttaaaaact ttagcagaat aattttgacg attaatgatt cagggcggtc
     7861 atcagagcaa cttcccatta atagagctgc aataaaggta ataagtaaag ttaatctcga
     7921 aatcatgatt tctattttta gttggatgat gtacggcaat attacttttt tatagctaat
     7981 ggctttattc aacatgaggt aaagaaaatg cttgagttgg gttaaaacaa tcctgtaatg
     8041 tttcctttta aatcaatatc aatattttct gctgcaggag ttgcgggcag accgggcatt
     8101 cgcataattt ctccggcgat cgggataata aaacctgcgc cggtagcaag ctcaatatct
     8161 ctaatgctca cagtgaaatc tgtcgggcgg ccaataagtg atgaattatc ggaaagtgaa
     8221 ttttgtgttt ttgcaataca aatcgccaaa tttgaaaacc caagtttttt aattcgttga
     8281 ataattttat tagcggaatt cgagtattca acgttagatg cgccatacat cttttgagct
     8341 atgattttga tcttttcgcg aatgggactc tccaattcat acagaaaccg aaaggcgtca
     8401 ttacttttta ctgcctctga tattaccaat tcggctaact ctaatgagcc ttcgccaccc
     8461 tttgcccaac cattgttaat agcaacattt acgcctttat ttttgcaatg gtttataatt
     8521 atgttcagtt cctcttcagt atcgcttgta aattggttaa tggttactat aggattaaca
     8581 ttgaaatgtt gaatgttttc tatgtgttta tctaagttct ccaatccatt aaaaacggcg
     8641 ttcgggttag gattattcag ttcttttctc tttactccgc catgaaactt taaagcacgg
     8701 actgttgcta cgattatggc cagatttggc gtaagccctc cgtatctggc ttttatatta
     8761 aaaaacttct cagctcctaa atcggatgca aaacctgctt cggtaataac gtagtctgac
     8821 agtgataagg ccattttagt gccaattacc gagttggttc cttgcgctat attggcaaaa
     8881 ggcccgccat ggataattgc cggagtattt tccattgttt gaaccaaatt gggtttaata
     8941 gcatgacgca atagtactgc catggcgcca tgggctttga ggtcgcgggc aaaaacaggc
     9001 tctttatcat acttacaacc aatataaatg cttccaagtt tgcacttcaa ctcctcaata
     9061 ttagcaacta aacataggat tgccattatt tctgaagcca cagctatatc aaaaccgctt
     9121 tggcgtggaa taccactcga ttgaccgcca agcccaataa tgactttacg taatgcacgg
     9181 tcgttcatat caataaccct tttccaggta atactgcgcg gatcaatatt cagattgttt
     9241 tttttgcttt gaatattatt gtcaatcagc gctgccagaa ggttgtgagc tttttcaata
     9301 gcggcaatat cgcccgtaaa atgtaaattt atgtcctcca tcggtagcac ttgcgacatg
     9361 ccgccaccgg cagcgccgcc tttaattcca aaaacaggcc caagagaagg ctctcgtatt
     9421 acaactacgg cattttttcc gattttgtta agagcttgcg ccaatgctat tgcaacggtg
     9481 gttttgcctt cacccgccgg tgtaggtgtc attgcggtaa ctaagataag tttcgatttt
     9541 ttagccctct cttcatttat atattcaagc gggatttttg ctttatacct tccgtacaaa
     9601 tccagatcgt caggattgat attcagtttt tctgcaatag cattaatatg tagcattttg
     9661 gcttcttttg ctaattcaaa atctgttttc attgtagatt gatttggtgt ggaaaattat
     9721 gagtaaacat tggttagcga tatgttttat taaggctctt atatgcaaaa tatattttg
//
LOCUS       DAOOAI010000083         9730 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_8704_length_9730_cov_5.228010, whole genome shotgun sequence.
ACCESSION   DAOOAI010000083 DAOOAI010000000
VERSION     DAOOAI010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9730)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9730)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9730
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8704_length_9730_cov_5.228010"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            49..1143
                     /locus_tag="PK345_06715"
     CDS             49..1143
                     /locus_tag="PK345_06715"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HPX34741.1"
                     /translation="MKRCLIVTGGGDCPGLNAVIRAVVKRASKERDWEIVGSIEAFNG
                     ILREPTEIVILDEKKVEGIHYRGGTIIQTTNRGGPFAWPIKNKDGTWSTVDRSEEMLR
                     KMQYLGIDAVINIGGDGSQRISKQLFDMGCNIIGVPKTIDNDLSATDFTFGFQTAVQI
                     ATEAVDKLVTTAASHNRVMILEVMGRYAGWIALNAAIAGGAEICLLPEFPYDINKVLD
                     NVNQRFERNRGFVNIVISESARAIGKDYTTLSEKEIGNEHRRLGGVANVLMHELKEAG
                     CEHSIRTTVLGHLQRGGIPIAYDRILATQFGVKAFEMVLNNEFGKMVSYRHPNIVSVS
                     LEEVLKQPKLVEADASLMNTARGVGIHFGD"
     gene            1148..2341
                     /locus_tag="PK345_06720"
     CDS             1148..2341
                     /locus_tag="PK345_06720"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007133829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HPX34742.1"
                     /translation="MDSIKQIYKIGHGPSSSHTMGPRKAAAQFKTKYPDASSVRITLY
                     GSLAATGKGHLTDVALEDELKPKKVEIIWNSKEVKPFHPNALMFEAFNDSEKKLGQWT
                     AYSIGGGDIVSDDRLENIEKSLYPHEKMMDILAFCEENGKHFWEYVQDYEDDDLWDYL
                     EEVWHVMKNAVLRGLENEGVLPGIIKLPRKASIYMIRARGFTGTLKRRSKLFSYALAV
                     AEENASGGMVVTAPTCGSSGVLPAVLYHMHTAYGFTDNKILRALATAGLIGNLVKYNA
                     SVSGAQVGCQGEIGTACSMATAAATQLFGGTPAQVEYAAEMGLEHHLGLTCDPIAGLV
                     QIPCIERNAFAAARAMDACNFAIMSDGKHRVSFDKVVAAMKQTGKDLPSLYRETSEGG
                     LAVVH"
     gene            complement(2375..2956)
                     /gene="rdgB"
                     /locus_tag="PK345_06725"
     CDS             complement(2375..2956)
                     /gene="rdgB"
                     /locus_tag="PK345_06725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009318369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HPX34743.1"
                     /translation="MKKFIFATNNPHKLEEIRQLVKNEFIILSLSDVGFNIDIPEDFD
                     SLQENALQKARIVHQMTGLDCFADDTGLEIDALDKRPGVNSARYAGPECNSEKNIRKV
                     LKELGDTPFRSARFKTIIALILNNKEFLFEGIVEGEIIYEKHGLFGFGYDPIFRPEHH
                     LVTFAEMSAEDKNRISHRGKAVEKLIKFLRNSM"
     gene            complement(3153..4472)
                     /locus_tag="PK345_06730"
     CDS             complement(3153..4472)
                     /locus_tag="PK345_06730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34744.1"
                     /translation="MKIKNLVFLLIAALLFACKPELDNITTTDGQADFSKYVAIGNSL
                     TAGYTDGELFLSGQNNSYPSIIAHQMLAAGGGEFKQPLMLDEYGFGKRLLLNAKAQAP
                     VPAGKPVNMANFNSIASQGPFNNLGAPGARSFHLIPGAEAFSALNPYYKRFAAQPGTS
                     TILDEALAQNPTFFSLWIGANDVLGYAISGGASDSITNPALYQMALDVILQQLTKSGA
                     KGIVANIPSITSTPYFAFMNTRLPFDGLVLKLEQQVAALNYGYAQLNALIKMMGSTDT
                     IAFKLGANPFVIVDKDLPWGIRQMKAGELFLLRLPTDSIANHGLGSLIPIPDMHVLTQ
                     LEIDRVETATYNYNAIILQLASQYDLAHVDTHGLLEKISKDGIIYDGIKFTSSYVTGN
                     TFSLDGVHITGQANAMVANEFIKAINSKYGSQLKEVSPLYYSGLYYY"
     gene            complement(4492..5715)
                     /locus_tag="PK345_06735"
     CDS             complement(4492..5715)
                     /locus_tag="PK345_06735"
                     /inference="COORDINATES: protein motif:HMM:NF015316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein transport protein"
                     /protein_id="HPX34745.1"
                     /translation="MKKIVTLLLLGCLVSSSLFAGGYQVRLQGQRQTGMGLIGTSLYG
                     DASNLFYNAAGLSKLNGKFSFAAGFSPIFNEIAFRLENDPVTVYNDNPVGTPFSVYGS
                     MMITKRLGFGIGAYTPYGSTAIWGNDWVGATLIQDLSLQAVFIQPTISYNFADVVSIG
                     AGLVIASGEVELNKAIPYNGAEVKGQITLNGKTTEYGYNVGLLITPNKKWAIGANYRS
                     EIIMSVQDGDATFTIPGSLYSIIPANNKFDADLPLPANFDFGLSFKASEKLLLAAEIN
                     YVFWDVYDSLNFTFKKMPAILNSSNPRLYSNTLLTRVGAEYTFNEVVKGRAGIYYDPT
                     PTHEEYFSPETPSLSSFAFTLGISLYPIENFAIDISYLQLETKQEFKTYKPSNFQGDY
                     KSRTLIPGVGLTYTF"
     gene            5970..7088
                     /locus_tag="PK345_06740"
     CDS             5970..7088
                     /locus_tag="PK345_06740"
                     /inference="COORDINATES: protein motif:HMM:NF013974.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34746.1"
                     /translation="MKREESKGMALIEGSRLIMEALARAGADSYIGYPITPANLLYQY
                     GGQRLDIMLAAPDEISTLQWMSGLSSTGRIPVTATSFPGFALMIESINMAYMMELPMI
                     IILVQRLGPSTGTATNGAQGDLLLLKGMISGGHPIPVLCTSSFEDSWSLAAETVKMAV
                     EMRTPVVLLTSKEEVMTHKSFDISKLDEIEKVDHKFYDNAEQYKSYLPKDMVPEFLPL
                     TQNRHQVRINASTHDQMGILQHSTDEALANTRRLEEKIYHRMKCHLKFDYDQHENAEK
                     IVLSYGITASAAREAVAQMRAEGEKVSLLIAKTPLPVPPVYLEILKKYKRVVIAEENM
                     NNQFGSLLFGINKPEGLRGAGALGKMITPQQIIEEVKA"
     gene            7085..8353
                     /locus_tag="PK345_06745"
     CDS             7085..8353
                     /locus_tag="PK345_06745"
                     /inference="COORDINATES: protein motif:HMM:NF014794.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPX34747.1"
                     /translation="MNIKILNTDSLPFCKGCGHNLISKNTAKALERMELSPLDVIMVT
                     DIGCHGIIDKTLNTHTIHGLHGRSVALGAGIVFGLKEPGKKIIVFIGDGGATIGLQHI
                     MEAARLNLNMSVVVHNNMLYGMTGGQSSGLTPEGFRTTTSADGSPFSGYDICALAHTA
                     GAAYVTRVPGIGDISEKLVKTFSTEGFSLMEVVEICPSYGIKFNPGMKLNEIIETSGR
                     KPGEWFNNRPVFTHHKGKKSENLLSKTPIIEPLFSSSLDKPVSLILSGSAGEGVQLTA
                     TVIAKAAMRSGLHVTQKGSYPVTVGVGFSTAEINLSKEDIHFHGINIPDLVVITSKEG
                     LNHSKRRIGLMKKGALFIDQTLDVPDTGAEIITEDYRGIGARTASLLAAIKCVTKTKI
                     LTYEAIFYTIEAEGLDKKLPVEKIKTALGL"
     gene            complement(8360..>9730)
                     /locus_tag="PK345_06750"
     CDS             complement(8360..>9730)
                     /locus_tag="PK345_06750"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HPX34748.1"
                     /translation="ILDEAQRIKNYESDISVSISSIPRKHTLLLSGNLYNYHLIELYA
                     LMMHVDQELLSPLWDFSYRYCIFDNHSSNRIIGFYNLDELDQRIEKIVLHRTKEQVIR
                     HLPKVSYVDLPVPLHPVQIKIQLGLAKELLEILQKKIISQFELQQAVELIRKLRMVAD
                     SSFLVDDMTNLSPKTEELKSLLTERLNIRKKRKKVIIFTEWKRMAQVISRVLRLSKIK
                     FVEITDETPEKQRTLSLKSFTTDPDCLVLVSSCATLDESEIKASDIVIHFDIPADRSL
                     KSLRMGSISGIIQRDGNLTCFNLYSKNSIEERLNAGMEINLHEKGKKKETDIPTEIPK
                     LIKSELIETLTDIINQGSEADESIQETLQRDESGQMVIDFASEEEISKVQKKSTSELS
                     DFVFSDSLDLDEDKILQTLANSTSLVAEMLKLATSDKADVQNYSPDFDPENGEILLRF
                     KLLRKA"
BASE COUNT         2943 a         1849 c         2084 g         2854 t
ORIGIN      
        1 gaataatcaa atgattatca agaaattatt aataaaaaag atttgcgtat gaaaagatgt
       61 ctgattgtta cgggtggggg tgattgccct ggactaaatg ctgtaataag agcagtagta
      121 aaacgtgcct caaaagagcg cgactgggag atcgttggga gcatagaggc ttttaatggc
      181 attttgcgtg agcctacaga gatagtaata cttgatgaaa agaaggtaga aggtattcat
      241 taccgtggcg gtacaattat tcaaacaacc aacagaggag gcccctttgc ctggcctatt
      301 aaaaataaag acggcacatg gagcaccgtt gatcggtcgg aagagatgct gcgaaagatg
      361 caataccttg gtattgatgc tgtaataaat attggtggcg atggttctca gaggataagt
      421 aaacagcttt tcgatatggg ttgcaacatt attggcgtac ctaaaactat tgacaacgat
      481 ctttcagcta ctgatttcac ttttggattc cagacagcgg tgcaaattgc tactgaagct
      541 gttgacaagt tggttacaac tgctgccagc cataatcgag tgatgatact tgaggtaatg
      601 gggcgttatg caggatggat tgctttaaat gctgctatag ccggtggagc agagatttgc
      661 cttctgccag agttccctta cgatataaac aaagtgttgg ataatgtaaa tcagagattt
      721 gaacgtaacc gcggttttgt aaatattgtg atatctgaaa gcgctcgtgc aataggaaaa
      781 gattatacca cgcttagtga aaaagagata ggtaatgaac atcgccgact tggcggtgtt
      841 gccaatgtgc tgatgcatga gcttaaagag gccggttgcg aacatagcat ccgaactact
      901 gtgttaggtc atctgcaacg cggtggaata cctattgcat acgatcgcat attggcaacc
      961 cagtttgggg ttaaagcgtt tgaaatggta ctgaataatg agtttggcaa aatggtgtcg
     1021 taccggcatc cgaacatcgt ctctgtatca cttgaagagg tgcttaaaca accaaagctc
     1081 gtagaagctg atgcatcatt aatgaatact gcccgtggag ttggaataca ttttggagat
     1141 taaatatatg gattctataa aacaaattta taaaataggg catggcccat cgagtagcca
     1201 taccatgggg ccgcgcaaag cggctgcaca gtttaaaacc aaataccccg atgcgagttc
     1261 agtccgtatt acattatatg gaagtttggc tgcaaccggt aaggggcatc tcacagacgt
     1321 tgcgctcgaa gatgaactaa aaccaaaaaa ggttgaaata atttggaact ctaaagaagt
     1381 aaaaccattt cacccaaatg ctttgatgtt tgaagctttc aacgatagcg agaaaaagct
     1441 tggccagtgg acggcctaca gcattggcgg cggtgatata gtaagcgatg atagattgga
     1501 aaacattgaa aaatcactct atcctcacga aaaaatgatg gatatccttg ctttttgtga
     1561 agaaaacgga aaacattttt gggaatatgt tcaggattat gaggacgatg atctatggga
     1621 ttatttggag gaggtatggc atgtaatgaa aaatgcagtg ttacgaggat tggagaatga
     1681 gggtgtgctt ccgggaatta ttaagcttcc aaggaaggca tctatctaca tgatcagggc
     1741 gagggggttc accgggacgt tgaagcgacg gagcaaattg ttttcttatg cgttagccgt
     1801 tgctgaggag aatgcttctg gcggaatggt agttaccgca cccacatgtg gctcatctgg
     1861 cgtattgccg gcggttttat atcacatgca cactgcgtat ggatttactg acaataaaat
     1921 tttaagagca ttggccaccg ctgggttaat aggtaactta gtgaaatata atgcttcggt
     1981 atctggtgcg caagtgggct gtcagggcga aataggaact gcctgttcta tggcaaccgc
     2041 cgctgccacc caactctttg gaggcacacc cgcacaagtt gaatatgccg ctgaaatggg
     2101 acttgaacat catcttggac ttacctgcga tccaatagca ggccttgttc aaataccatg
     2161 catcgagcga aatgcttttg cggccgcacg cgcaatggat gcctgtaact ttgcaattat
     2221 gtcggatgga aaacacagag tgtcatttga taaggttgtt gctgccatga aacaaacagg
     2281 aaaagacctg ccgagcctat atcgtgaaac ttccgaagga ggacttgctg ttgtgcatta
     2341 attacaatct gcaattccat tgaacttgag aggctcacat gctatttctt aaaaacttaa
     2401 ttagcttctc aacagctttt ccacgatggc tgattcggtt tttgtcttca gcgctcattt
     2461 cggcaaaagt aactaaatga tgttcaggcc tgaatattgg atcatatcca aagccaaata
     2521 gtccatgttt ttcgtagatg atctcgccct ctactattcc ctcaaacaga aactccttat
     2581 tatttaagat taaagctatt atagttttaa atcgagctga cctgaaagga gtgtctccca
     2641 actcttttaa cactttcctg atgttttttt cagaattaca ttccggtccg gcataacggg
     2701 ctgagttaac tcctggacgt ttgtcaagtg cgtctatttc aagcccggtg tcatcagcaa
     2761 aacaatccaa tccagtcatt tgatgtacaa ttcgcgcttt ctgtaaagca ttttcctgca
     2821 aagaatcaaa atcttcaggg atatcaatat taaatcctac atcgctaaga ctgagtatga
     2881 taaattcgtt ttttacaagc tgacgaatct cttctaactt atgcgggtta ttagtggcaa
     2941 atatgaactt tttcaattta gtttatttga aaattttaga attaaagaat gttgatcagg
     3001 caattgttaa aaggttacag gggtgttgca taattcaggt ataatattac atcctttaaa
     3061 actgactttg aaacttttac tttttttata aaaatacgaa ctcccgtcag agtttaatac
     3121 tgacggaatt tgctactttt tcaaagatgt aattaataat aatatagccc cgaataatat
     3181 agagggctaa cttctttgag ctgtgagcca tattttgaat taattgcttt aataaattcg
     3241 ttggctacca ttgcattagc ttgtccggta atgtgaaccc catctaatga gaaggtgttg
     3301 ccggtaacgt atgacgacgt aaacttgata ccatcgtaaa tgataccgtc ttttgatatt
     3361 ttttccaaca aaccatgtgt atccacgtgt gctaagtcgt attgacttgc caactgaagt
     3421 ataattgcat tataattata ggtggcggtt tcaactctgt caatctctaa ctgtgtaagt
     3481 acatgcatgt cggggatagg aattaggctt cctaaaccat gattagcaat tgagtctgtt
     3541 ggtagtctca gcaaaaataa ttctccggct ttcatctgcc ttattcccca tggtaaatct
     3601 ttatctacaa tcacaaaagg atttgctccc agtttaaatg ctatggtatc ggttgatccc
     3661 atcattttta ttaaagcatt aagctgtgcg tagccgtagt taagcgctgc aacttgttgc
     3721 tctaatttca gtacaagccc gtcaaaaggc agtcgcgtat tcataaatgc aaaatatgga
     3781 gtacttgtga ttgatggtat gttagccaca atgccttttg ctccgctctt agtgagttgt
     3841 tgcagtatta catccagtgc catttgataa agggctggat tagtgatact atcggaagcg
     3901 cctccggaaa ttgcatatcc taatacgtca ttcgctccta tccataaact aaaaaaggta
     3961 ggattttgag caagcgcttc atctaatatt gtagatgttc cgggttgagc ggcaaatcgt
     4021 ttataatatg gatttagagc gctgaatgct tctgcaccgg ggatcagatg aaaagatctt
     4081 gctcctggcg ctccaaggtt gttgaaaggc ccttgtgaag ctatggaatt gaagttagcc
     4141 atatttacag gtttccctgc gggaacagga gcttgagctt ttgcatttaa caataatctc
     4201 tttccaaacc catattcgtc gagcatcagc ggctgcttga actcgccacc gccggcagct
     4261 aacatttgat gagcaatgat tgagggatag gaattattct gacctgacag gaaaagttcg
     4321 ccgtcagtat atcctgctgt taacgagttg ccaatggcta catatttcga aaaatcggct
     4381 tgtccatcag tagtagttat attgtcaagc tcaggtttgc aagcaaaaag aagagctgct
     4441 attaaaagga aaactaaatt ttttattttc atgattttca attgttaaac gttaaaaagt
     4501 ataggttaat cccacaccgg gaatcaaagt tctggatttg taatctccct gaaaattgga
     4561 gggtttatat gtcttaaatt cttgttttgt ctccaactgt aggtatgaga tatcaatggc
     4621 aaaattttca atcggatata atgaaatacc cagtgtaaag gcgaaggaag aaaggcttgg
     4681 cgtttcgggg ctgaaatatt cctcatgagt aggtgtagga tcatagtaaa tgccagctcg
     4741 gcctttcact acctcattaa aggtatattc cgctccaact cgtgtgagca aagtgtttga
     4801 atatagcctt ggattgcttg agtttaatat ggcaggcatt tttttaaagg taaagttcaa
     4861 tgaatcataa acatcccaga aaacataatt gatctcagcg gcaagcaaca acttttcgga
     4921 ggctttaaaa gagaggccga aatcaaaatt ggcaggcaga ggaaggtctg catcaaattt
     4981 attgttggca gggatgatag agtagagcga tcctggaatg gtaaatgtag catcgccatc
     5041 ttgtaccgac ataataattt ccgaacgata attggctcct atcgcccatt ttttgtttgg
     5101 tgtaatcaac aacccaacat tatatccgta ttcggttgtt tttccattaa gcgttatttg
     5161 tccttttact tcggcaccgt tataaggaat agctttattt agctcaactt cgcctgaggc
     5221 tatcactaaa cctgctccaa tgctcaccac atcagcaaaa ttgtaggaga tagttggttg
     5281 tataaatacg gcttgcaacg ataagtcttg aatcagagtt gccccgaccc agtcgttacc
     5341 ccatatagca gtactgccgt aaggagtata agctccgata ccaaaaccta atcttttagt
     5401 aatcatcata gagccgtaaa cactaaaagg cgtaccgaca ggattgtcgt tgtaaaccgt
     5461 tacagggtca ttttcaagcc tgaaagcaat ctcgttgaaa atcgggctaa atccagcagc
     5521 aaagctaaac ttgccattta acttagataa tcccgcagca ttgtaaaaaa ggttgctcgc
     5581 atcaccatat agcgaagtac cgataagccc catcccagtt tgacgttgac cttgaagcct
     5641 tacctgataa ccacctgcaa acaggctgct cgatacaaga caacctaata gaagaagtgt
     5701 aacaattttt ttcataaact tcatgatttg atagattaaa aattcagcga gcgaaaatac
     5761 aataaatgta tctgtgtgtg aaggttagca aatattattc aataaaaaat gttaattaac
     5821 gatcaaataa catttttaat aggatttggt taattaaatt ggattattta aaatcgaaag
     5881 aaattattac ggaatggttg aatagagaaa aataaatcgg acttttgcaa aaaatctaaa
     5941 tcttttttta ttaaaataat tttgtgctaa tgaaacgaga agaatcaaaa ggcatggctc
     6001 tgatagaggg atcaaggttg atcatggaag ctctcgcccg tgcgggcgct gattcctata
     6061 tcggctatcc aatcacaccg gccaacttgc tataccaata tggtgggcaa cgtttggata
     6121 tcatgttggc cgcacctgac gagatatcta cacttcagtg gatgtcgggg ctatcttcga
     6181 cagggaggat tcctgttacg gcaacctctt ttccgggttt tgccctgatg attgagtcaa
     6241 tcaacatggc atacatgatg gaacttccaa tgattattat ccttgtgcaa aggcttgggc
     6301 cttcaaccgg cacggcaact aacggcgctc aaggtgattt gcttttgcta aaagggatga
     6361 tttcaggagg gcatcctata ccggttttat gtacttcaag ttttgaagat agctggagcc
     6421 tcgccgcaga aacagtgaag atggccgtag aaatgaggac gcctgtggtg cttttaactt
     6481 caaaagaaga agtgatgacc cataaaagct ttgatatatc caaacttgat gagattgaaa
     6541 aagtagatca caagttttac gataatgctg agcaatacaa atcatatttg ccaaaagata
     6601 tggttcctga atttctaccg ttgacgcaaa atcgccatca ggtaaggatc aatgctagta
     6661 cacatgacca gatgggaatt ttacagcatt ctacggatga agctctagca aacacacgtc
     6721 gccttgaaga aaaaatatac catcgaatga agtgccattt aaaatttgat tacgatcaac
     6781 acgagaatgc tgaaaagata gtactctcgt atggcattac agcatccgca gcacgtgaag
     6841 cggtagcgca aatgagggca gaaggagaga aggtatcatt acttattgcc aaaacaccat
     6901 tgcctgttcc tcctgtttat ctcgaaattc ttaaaaaata taaaagagtt gtcatagccg
     6961 aggaaaacat gaataatcag tttggctcgt tgctttttgg cattaataaa ccggaaggat
     7021 taaggggtgc cggcgcgtta ggaaaaatga ttacaccaca acaaattatt gaggaggtaa
     7081 aagcatgaat ataaagattt taaataccga ttctttgccc ttttgcaaag gctgtgggca
     7141 caaccttatc tctaaaaata cagctaaagc tttggaaagg atggagctat cgccacttga
     7201 tgttattatg gtaaccgata tcggatgcca tggcattatt gacaaaaccc tcaacacgca
     7261 tactatccac ggattgcatg gccggtcagt agcgctcggc gccggaatcg tttttggatt
     7321 aaaagagcct ggaaaaaaga tcatcgtctt tattggtgac ggaggagcta ccattggttt
     7381 gcagcatata atggaagccg cacgcctcaa tttgaatatg tcagttgttg tgcataataa
     7441 tatgctttat ggtatgaccg gcggccagtc cagtggactt acacccgaag gatttcgtac
     7501 tactacatca gccgatggaa gtcccttttc aggatatgat atttgcgcgc ttgcgcatac
     7561 tgcaggagct gcttatgtta cccgtgtgcc tgggatagga gatatttctg aaaagcttgt
     7621 aaagactttt tcaacagagg gattctcgct gatggaggta gtagaaatat gcccaagtta
     7681 tggtattaag tttaacccgg ggatgaagct gaacgaaatt atcgaaacct ctgggagaaa
     7741 gccgggtgag tggtttaaca acagacctgt ttttacacat cataagggta aaaaatcaga
     7801 gaatctatta agtaaaacgc caataattga acccctcttt tcttcttcgc ttgacaagcc
     7861 tgtttcatta atattgagcg gatctgccgg cgaaggagta caactgacag ctactgtaat
     7921 tgccaaggca gccatgcgtt caggattgca tgttacacaa aaaggaagtt atccggtaac
     7981 tgtcggggtt ggattctcta ctgccgaaat aaatttatcc aaagaagata ttcactttca
     8041 tggtatcaat attcctgacc ttgttgttat tacatcaaag gagggcttaa atcatagcaa
     8101 aagacgtatt ggattgatga aaaaaggagc gttattcatt gatcagacgc ttgatgtacc
     8161 cgataccgga gcagaaatta taacagagga ttacagagga ataggcgcca ggacggccag
     8221 cttgcttgca gctataaagt gtgttacgaa aaccaaaatt ttaacctacg aagcaatatt
     8281 ttatactatt gaagcagaag gcttagataa aaagttgccg gtagaaaaaa tcaaaacggc
     8341 tttaggttta taaaacttat caggcttttc gtaataattt gaaacgaagt aatatttcac
     8401 cattttcagg atcgaaatca ggtgaataat tctgaacatc tgccttatca cttgttgcaa
     8461 gttttagcat ttccgcaact aacgacgtgc tgtttgcaag tgtttgaagg attttatctt
     8521 catctaaatc aaggctgtca gaaaaaacaa aatctgatag ttcggatgta ctttttttct
     8581 gaaccttgga tatttcctct tcgctagcaa agtcaatcac catctgcccc gattcatcgc
     8641 gctgtaaagt ctcttgtata gattcatcag cctctgatcc ttgattgata atatctgtca
     8701 atgtttcaat tagttcagat tttatcagct tgggaatctc cgttggaata tcggtttcct
     8761 tcttcttgcc cttttcatgc agattgattt ccataccggc attgagcctc tcttcgatgg
     8821 aattttttga atatagattg aaacaagtca gattgccatc gcgttgaata atcccggaaa
     8881 tactgcccat cctaaggctt ttcaaactac gatcggctgg aatatcgaaa tgtattacta
     8941 tatcggaagc ttttatctcc gattcatcaa gggtagcaca agaagatacc aacactaaac
     9001 aatcgggatc ggtagtgaat gatttcaatg agagagtgcg ttgtttttcc ggcgtttcgt
     9061 ccgttatttc aacgaatttg attttgctaa gccttaaaac cctgctaatc acttgcgcca
     9121 tccgtttcca ttcagtaaag atgatcacct tttttcgttt tttcctaata ttcaaccttt
     9181 cggttaaaag gcttttcaac tcctccgtct ttggagatag gtttgtcata tcatcaacaa
     9241 ggaatgatga gtcggctacc attcttaatt tacggattaa ctctacggcc tgctgtaatt
     9301 caaactgact tatgatcttc ttttgcaata tttccaacaa ctcttttgct aagcctaact
     9361 gtatttttat ttgaaccgga tggagtggca caggcagatc aacatagctg actttgggca
     9421 aatgccgtat cacttgctct tttgttcgat gaagaactat cttttcaatc ctttgatcta
     9481 attcgtcgag gttgtaaaaa ccgataatcc tattgctgct gtgattgtca aaaatgcagt
     9541 acctgtacga aaaatcccat aatggcgaaa gcaactcctg atcaacatgc atcatcagtg
     9601 cataaagttc aatcagatga taattataaa ggttccccga taaaagtaga gtatgcttgc
     9661 gaggaataga actgatagat accgaaatat cagattcata atttttaata cgctgagctt
     9721 catctaagat
//
LOCUS       DAOOAI010000084         9647 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_8795_length_9647_cov_5.364053, whole genome shotgun sequence.
ACCESSION   DAOOAI010000084 DAOOAI010000000
VERSION     DAOOAI010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9647)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9647)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9647
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8795_length_9647_cov_5.364053"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..1572
                     /locus_tag="PK345_06755"
     CDS             88..1572
                     /locus_tag="PK345_06755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPX34749.1"
                     /translation="MEKNKKKKNGLDRRSFLRNTAVASAGFIILPSHVISGLGHRMPS
                     DKLNIAGIGVGGRGGGVIRAVKSENIVALCDVDWKYASKIFGDFPDAKKYYDWRVMFD
                     EMSDSIDAVVVGTPDHTHAIISINAMKLGKHVYCEKPLTHSVWESRQMTEIARQYKVA
                     TQMGNQGNSGEGIRQVCEWVWDGAIGEIKEAHAWTNRPIWPQGLERPKEVHPIPDTLK
                     WDLFLGPAKERPYNPIYTPWNWRGWWDFGTGALGDMACHILDPIFMSLKLKYPIKVQG
                     SSSLFNTESPPLAEVVKYTFPAREKFRNLEMPEVTVTWWDGGLLPPRPEELPDGEIMG
                     RDTSGGVLLIGTKGKIMSGCYGRNPFLLPLDFDKSYKRPAPELRRITVGHEMDWVRAC
                     KESPESRIEASSHFGYSGPMNEMVVMGVVAVRLQDLKRELLWDGQKMQFTNISDNDEI
                     RVVLSDKFEIIDGHPHFDTKHETINAKQAAEEYIRHTYREGWSL"
     gene            1624..2382
                     /locus_tag="PK345_06760"
     CDS             1624..2382
                     /locus_tag="PK345_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1080 domain-containing protein"
                     /protein_id="HPX34750.1"
                     /translation="MKTFKYLILITIFAVACGGSQQKATDEKVEEITDNTLTQSEIDD
                     GWILLFDGKEAGKWRGYNKDYFPNGWEISDGMIRCIGSGRGEAGGLEGGDIIYDEKFQ
                     NFELTLEWKISPGGNSGIFYLAQESPDLTIWQTAPEMQVLDNTTHPDGREGLHSAGAL
                     YDMIGVEQDKVKPVGEWNQVKILVFKGLVEHWLNGEKVVSYHLWTPEWYEMVANSKFP
                     KYNPNWAEVAQEGYIGLQDHGDDVWYKNIKLKKL"
     gene            2809..3372
                     /locus_tag="PK345_06765"
     CDS             2809..3372
                     /locus_tag="PK345_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HPX34751.1"
                     /translation="MDEAFLLLDKYFGRGSLAHKIIIAHSVAVKDMALKIASKNPHLK
                     IDISLLTKAALLHDIGAIKTNNPSIGCYGDLPYICHGYKGREILENEKLYDVAPFCER
                     HTGTGITKEEIIKLNLPIPHRDMVPITIEEKILCYADKFFSKSGLNLTEPKKLKNIYS
                     NLIRYGEDKARKFDEFIGMFGIYYVYI"
     gene            complement(3421..4815)
                     /locus_tag="PK345_06770"
     CDS             complement(3421..4815)
                     /locus_tag="PK345_06770"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34752.1"
                     /translation="MKNLKILFLSISLLFIVLSLEAQYFSLPDSIFDDMIGGKYVLDS
                     TYSYEWSVLNHGWMNTDRLFITERDKYGNQKRSKIIKWDEENSKWDDWKRNEQAYYDS
                     LHSHFFIAFIYDLKAGDWKMSDSIFYNRKGKPEFSWFKVWDPDKYRFSRGKRVKFQYT
                     SADLVSKEEIDIFDTISDNWKANQVVSYQYNEDHLLEVQQVKVWKDSGFWRDSLKITY
                     SYNNQLQNISILQEKFNDNDFWENEQLTNKEFDENGNQYEVYKYRWNTTLQQWDNWNR
                     YFYNYESNSLLIETVQQYWENDFQKWINLNKTIYEYNSLLQKTSVLQQFWDAFGNFWL
                     NTSNYTYSYDDAGNRTGFLFRFWDEEYSEWLNIYKDANWWSFFDPSSISEFQINKVIV
                     FPNPATSDINIAINHPFQDCYLTIYANDGQIVYSEIIKNSQVRIDISQFPKGVYILRL
                     VIDRETTTGKIMVY"
     gene            4915..7230
                     /locus_tag="PK345_06775"
     CDS             4915..7230
                     /locus_tag="PK345_06775"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FG-GAP-like repeat-containing protein"
                     /protein_id="HPX34753.1"
                     /translation="MIRTLFIALLFSILLFSNVSFGQYQLPFGFEKIYDVAVYDESQS
                     LFKNPWGGGLFAVQFNEIDLDGDGIKDLVVFDRCGSRTLTWKNLGIPGEVSYQFAPEL
                     AKILPSFSDWVIFADYNNDGLADIFTYSKGFAGIKVYRNNGLTAEEPFELIEPYYLTS
                     YQGNGYVNILVTYVDYPAIIDIDGDGDLDILTFWGLGSFVELHTNESMELYGVPDSLI
                     FRKTTNCWGWFAESEESNQLYLDTCFILPKQKIKDDIKMLADRAEYRHTGSTFLIFDE
                     NDDGLPDLLLGDVDYPAPALLINSGTVGEAVMGSYTFNWPAYDIPIDLLSFPLPALID
                     LNNSGRKDLVVTTFDPGFIKSQNYENIWYYEDTNTAGAPEFKLKTKSFLQDEMLDFGA
                     GAAPLFFDYNGDGLLDLLIGNFGYLDTSYYDAGLNLNCKYRGQLALLENCGAPTNPQF
                     KLTNRNFADLPSYFPVGERPYAIIPATADLDNDGDMDLLLGNAKGTLIFLENIGSPNQ
                     PAQFILKEQNYQNISVGKFSAPQLIDLTGNGLPDLVIGKRNGTISFYQNTGTAEKPVF
                     SFVTDSLGGVDVRNPNLSYYGYCVPHFFKDSIGKTHLFAGSEFGEIFYYTDIDNNLDG
                     QFTLVMKNYLWIDEGLRSTVALANLNNDEYIDMIVGNFSGGLSFFSGTKAPPDGLSKN
                     QLPEINIRLYPNPAKDFIIISIEETATAKFNSVRLLDFMGKELNLNTKSSSAELYLNI
                     EKIPVGVYILLIDVLHHNNILFTKPIKFIKT"
     gene            7227..>9647
                     /locus_tag="PK345_06780"
     CDS             7227..>9647
                     /locus_tag="PK345_06780"
                     /inference="COORDINATES: protein motif:HMM:NF012340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HPX34754.1"
                     /translation="MKYQLLVIVIFIFFVNGARSQLIPEKAAVDTSYSISTEEFEKMK
                     NNGIVLPPIKPADDRWANLHYKSSRELPLMYDMRQTPWLTSVKTQSAGGCWAYSVMGT
                     VEARLLMLDKGYYDLSDNNLKICHKYLPERNTYGNHWMATSYFARHSGPFLESDDPYP
                     GGTTAPENCPNDLTPLFYIGNSRYPPPLDKLFIKQTVLEYGPVWTLMYYHADFFKPTN
                     ATYYYGGNKQVNHAGVIVGWNDTLQTDGGSGAWIVKNTYGPNWGEGGYFYVSFFDSQF
                     LKYNSYWPEITENEDSTYLYQYDEVGGYWGVGFGNEIGYGLVKFEAIEGISEIIKVGT
                     FVQYAGSGIEIKIYGNFDGQISDLLLSKDEVICELPGYYTFDLDSTIYIPQGKSFYVQ
                     VKYNSMNPENKWPVSVEDTIINYCNPQIESGKFWIAPNPDIWPTAWYQVGAGTEYHYD
                     LCIKAYSRQIPMPPAPIAEAGEDITIIEGESAQPENAIAENYSSLLWSSNGDGVLINE
                     TTPNPTYIPGPQDIYKGTVNLIFQVISLPPLYTVKTDTLKVFILRYPTVELTFPQHGQ
                     KLCDPQIIITGNAYDPDGDLQSVDYSLNNNEWLTSGNSGTWSATITLSPGANTLKARA
                     TDAYGLSKETEEIKVICSIQNIFLPQGWSLISGFLLPDETEIAKMFDSVASNLILLQN
                     TNGIFAPPPININTLSNWDIHSGYKLKMAADDELIFCGNIPDNNQLELSSGYKLIPYL
                     SNQPATIDQLFQNPATDILYVFDLTNSLIYWPVGEIYTLTTLFPGIGYQAFFINQKNS
                     FTNCKTNPP"
BASE COUNT         3044 a         1872 c         1925 g         2806 t
ORIGIN      
        1 acactatagt gaaggggcgc cgctttattg tgcacaacaa agtattcaat atattcaatc
       61 acattttttt aactaaaacg atgaactatg gaaaagaaca aaaagaagaa aaacggcctt
      121 gatcgccgtt catttttacg aaacacagcc gttgcttctg cagggtttat catcttgcca
      181 agccatgtca tttccgggtt agggcatcga atgccaagcg acaaattgaa catagccgga
      241 attggtgttg gtgggcgagg tggtggagtt atacgtgcag taaaaagcga gaatattgtg
      301 gcgctatgcg atgttgactg gaaatatgct tccaaaatct tcggtgattt tccggatgct
      361 aaaaagtact acgattggag ggtgatgttc gacgagatgt ccgattcaat tgacgctgtt
      421 gtagtaggaa cgcccgatca tacacatgct attatatcca ttaatgcaat gaaacttggc
      481 aagcatgtct attgcgaaaa gcctcttact cattcggttt gggagtcaag acaaatgacg
      541 gagatagccc gccagtataa agtagctaca cagatgggca atcagggcaa ctcaggcgaa
      601 gggatcagac aggtttgtga gtgggtatgg gacggcgcca ttggcgagat aaaagaggct
      661 catgcctgga ctaaccggcc gatatggcct caaggtttag agcgaccaaa agaggtacat
      721 ccgatccctg acaccctgaa atgggatctg ttccttggcc cagcaaaaga gagaccctac
      781 aacccaattt atacaccatg gaactggcgc ggttggtggg actttggtac aggagcgtta
      841 ggtgatatgg cttgccacat tttggatcct atatttatgt cgcttaaatt aaaatatcct
      901 atcaaagttc agggtagctc atcacttttc aatactgaat caccaccttt ggccgaagta
      961 gtaaaatata catttccggc acgagagaaa ttcagaaacc ttgaaatgcc tgaagttact
     1021 gttacatggt gggacggtgg cctgttgcct ccacgccctg aggaactgcc agacggcgaa
     1081 ataatgggac gcgacaccag tggaggcgta ttactcattg gcacaaaagg aaaaataatg
     1141 tctggttgct atggccgaaa tccattcctc ttaccgctcg attttgataa aagttataaa
     1201 aggccagcgc cggaattgag aaggattact gtaggacatg agatggattg ggtgagagca
     1261 tgtaaagaga gtcccgagag tagaattgaa gcgagttcac actttggata ctctggccct
     1321 atgaacgaaa tggttgttat gggagtggtg gcagtaagat tacaagacct aaaacgcgaa
     1381 ttgctctggg acgggcaaaa aatgcaattt accaacatct ccgataatga cgaaataagg
     1441 gtggtgctta gcgataaatt tgaaataatt gacgggcatc cgcattttga tacaaagcac
     1501 gaaactatta atgctaaaca ggctgccgaa gagtatatcc gacacaccta ccgggaagga
     1561 tggtcattat aaactataat tttattaaat acttaattat taacaattta aacacatttt
     1621 acaatgaaaa cttttaaata tctgatttta atcaccattt ttgctgtagc ctgtggtggc
     1681 agtcaacaaa aagctacaga tgagaaagta gaagagataa cggacaatac attaacacaa
     1741 tctgaaatag atgatggttg gatactactg ttcgacggaa aagaggccgg caaatggcgc
     1801 ggttataata aagactattt ccccaacgga tgggagattt ccgatggcat gatcagatgt
     1861 atcggttcgg gtagaggcga agccggcggg ttagagggtg gtgatattat ttatgatgag
     1921 aaatttcaaa attttgagct tacactcgaa tggaagattt cgccgggcgg taatagcggc
     1981 atcttttacc ttgcacagga gtcaccggac ttgaccattt ggcagactgc accggaaatg
     2041 caagtgctgg acaacaccac acatcctgac ggacgtgaag gcttacacag cgccggagca
     2101 ctgtacgata tgataggtgt agaacaggat aaagtaaaac ccgtaggcga atggaaccag
     2161 gtgaagatat tggtttttaa aggtcttgta gagcattggc tcaatggcga aaaagtagtt
     2221 tcgtatcacc tctggacacc cgaatggtat gagatggtag ccaacagtaa attcccaaag
     2281 tacaatccta actgggccga agtggcacaa gaaggatata tcggactaca agaccatggc
     2341 gatgacgtat ggtataaaaa cataaaactc aaaaagttat aacaattcgg tacaaactaa
     2401 tatcacctta acaggtttgt tgttctgatc tttgtaaccc aaattaatca atggaaaact
     2461 cactgatcta ttggttttat tgggctaact aaggagtagt gtttgttcag tgcaatctct
     2521 atttgcaggt tacaaacgcc taactttctt tgatactata taataactat ttatttagtt
     2581 atcaattaat tagctaatca agacttatct ttcgagaaat acaagctttt ttatgatcaa
     2641 agctactcac gaggtaactc atacattact cacagggtaa ctttgagtta ccctgtgagt
     2701 aacaagagtg ccaacccaat ataaaattac cctgtgagta aaacaattat cctatattcg
     2761 gataatcaac caatttaata tttttgcaat cacctgaaac aaatagagat ggatgaagct
     2821 ttcttattat tggacaaata cttcgggcga ggatctttag ctcataaaat aataatcgca
     2881 cattctgttg ctgttaaaga tatggcgcta aaaattgctt ctaaaaaccc acatttaaaa
     2941 attgatattt cacttttaac aaaagcggcg ttgcttcacg atatcggcgc tataaaaacc
     3001 aataatccca gcatcggctg ctatggcgat ctaccttaca tatgccatgg ctataaagga
     3061 cgcgaaatcc ttgaaaatga aaaactatat gatgttgctc cattctgtga gcgacatacc
     3121 ggtaccggaa ttactaaaga agagatcata aagctaaacc ttccaatacc tcatcgtgat
     3181 atggtaccca ttaccattga agaaaaaata ctctgttatg ctgataagtt tttttcaaaa
     3241 tcgggtctta accttaccga gccaaaaaaa ttgaaaaaca tttattcaaa ccttatccgt
     3301 tacggagaag ataaagcaag aaagttcgat gaattcatag gaatgttcgg aatctattat
     3361 gtgtacatat aatttttcca agtagatgct ttgtggtttt tcaatgtact tggaccttca
     3421 ttaatacacc attattttac cggtcgtagt ctctctgtca ataacaagcc ttaaaatata
     3481 aactcctttt ggaaattgcg aaatatctat cctgacctga gaatttttaa tgatttcgga
     3541 ataaacgatt tggccgtcat tagcataaat tgtcagataa caatcctgaa aaggatgatt
     3601 aatcgcgata ttgatatccg aagtggcagg attcggaaaa acgattacct tattaatttg
     3661 aaattctgat attgacgagg gatcaaagaa tgaccaccag ttagcatctt tataaatatt
     3721 cagccattcc gaatactctt catcccaaaa acgaaacaga aaccctgttc ggttaccagc
     3781 gtcgtcgtaa ctgtatgtgt agtttgaagt attgagccaa aaattgccaa aagcatccca
     3841 aaactgttgc aacacactgg ttttttgcaa caaagaatta tattcgtaaa tggttttatt
     3901 aaggtttatc cacttttgaa aatcattttc ccaatattgt tgaacagtct caatcagcaa
     3961 cgaattactt tcgtagttat aaaaataacg attccaattg tcccattgct gtaaagtagt
     4021 attccatcta tatttgtaca cttcatattg atttccattt tcatcaaact ctttattggt
     4081 aagctgttcg ttttcccaaa aatcgttatc gttaaatttt tcctgcaaaa tactgatgtt
     4141 ttgcaactga ttattgtaac tataggtgat tttaagggaa tctcgccaaa aaccggaatc
     4201 tttccatact tttacctgtt gcacttcaag caggtgatct tcattatatt gataagaaac
     4261 cacctgatta gctttccaat tatctgaaat tgtatcgaaa atgtctatct cctcttttga
     4321 aaccaaatcc gccgatgtat attgaaattt tacacgcttt ccgcgtgaaa atctatattt
     4381 atcagggtcc cacactttaa accaagaaaa ttcaggtttt cctttacggt tataaaaaat
     4441 tgaatccgac attttccaat cacccgcctt taaatcgtaa atgaaagcaa taaaaaaatg
     4501 cgaatgtaag ctgtcgtagt atgcctgttc gttacgtttc caatcatccc attttgaatt
     4561 ttcctcatcc cattttatta tcttcgatct tttctggtta ccatacttat cgcgctcagt
     4621 gataaaaaga cggtcagtat tcatccatcc atgattcagc acactccatt catagctata
     4681 agtagaatca agaacgtatt taccaccaat catatcgtcg aaaatagagt ccggcagtga
     4741 aaaatattga gcctccaacg ataaaacgat aaaaagtaag gagattgaca agaataatat
     4801 ctttaaattt ttcatcaaca taaattttta cgaagataca acgatttgca tcagaaagta
     4861 tatttttgtt gaagctcgga taataataaa ccttgttcgg aacagtaaat gaatatgata
     4921 agaactttgt tcatcgctct gcttttttct attcttttat tctcaaatgt atcatttgga
     4981 cagtaccagc ttccttttgg ctttgagaaa atatatgatg tagcagtata tgatgaatca
     5041 caatctttat tcaaaaaccc atggggcggt ggtttgtttg ctgttcaatt taatgaaatt
     5101 gaccttgatg gtgatggaat aaaagacctt gtggttttcg acaggtgtgg aagccggaca
     5161 ttaacgtgga aaaatcttgg gattccagga gaggtaagct atcagtttgc gccggagctt
     5221 gcaaaaattt taccctcttt tagcgattgg gtaatatttg ccgactacaa taatgacggg
     5281 cttgcagata tattcaccta ctcgaaagga tttgccggta tcaaggttta ccgtaataat
     5341 ggtttgacgg ctgaagagcc attcgagcta attgagcctt actacctcac ctcatatcag
     5401 ggaaatggct atgtgaacat ccttgttact tatgttgact atccggcaat cattgacatt
     5461 gatggcgatg gcgatctcga catactgacg ttctggggcc tgggctcgtt tgtagagtta
     5521 catactaacg aatcaatgga attgtatggc gttccggatt cattaatttt tcgtaaaact
     5581 accaattgtt ggggatggtt tgccgaaagc gaagaatcga accagttata tctcgataca
     5641 tgttttattt taccaaaaca aaaaatcaaa gatgatatta aaatgcttgc cgatagggct
     5701 gaatatcggc ataccggttc cacctttttg atctttgatg aaaatgatga cggtctgccc
     5761 gatctgctgt taggcgatgt ggactatcct gcgcctgcgt tgctaataaa ctcaggcact
     5821 gtaggggagg ctgtaatggg gagttatact ttcaattggc ctgcttatga cataccgatt
     5881 gatctactct catttccctt accggcgctt attgatctga acaatagcgg gagaaaagac
     5941 cttgtagtta ctacattcga tccgggtttt ataaaatcgc agaactacga aaatatctgg
     6001 tattatgaag atactaacac ggcgggggcg cctgaattca aactgaaaac aaaatcattt
     6061 ttgcaggacg agatgctcga ttttggtgct ggcgctgcac cgcttttttt cgattataat
     6121 ggcgatggac ttttggattt gctgataggt aattttggat acctggacac ctcttattac
     6181 gacgccggat taaacctaaa ttgtaaatat cgcggccagc ttgcattgct cgaaaattgc
     6241 ggggcgccca ccaacccaca attcaaattg acaaaccgca attttgctga tctcccctcc
     6301 tatttccctg ttggcgaacg accttacgct attatccccg ccactgccga cctggataac
     6361 gatggcgaca tggatctcct gcttggaaat gcaaaaggaa cattaatttt tctcgaaaat
     6421 atcggaagcc ctaatcagcc tgcacagttt attttgaaag aacaaaacta tcaaaatata
     6481 tccgttggaa agtttagcgc tccacagctc attgacctta ccggaaacgg gctacctgat
     6541 ttagttatcg gaaaaagaaa tggcactatc tccttttatc aaaacactgg cactgccgaa
     6601 aagcctgttt tttcatttgt taccgatagc ttgggcggtg tggacgtacg aaaccccaat
     6661 ctgtcgtact atggatattg tgtaccacat ttttttaaag atagcatagg gaaaacacat
     6721 cttttcgcag gctccgaatt tggcgaaata ttttactaca ccgatattga taacaacctt
     6781 gatgggcaat ttacgctggt gatgaaaaat tacttatgga ttgacgaggg cttgcgcagt
     6841 accgttgcat tagccaacct gaataacgat gaatatattg atatgatagt tggaaacttc
     6901 tcaggcggac tctccttttt ttccggaaca aaagcgcctc cagatgggtt aagcaaaaat
     6961 caattaccgg aaattaatat tcgactatac cccaatccgg caaaggattt tatcattata
     7021 agcattgaag aaactgctac agcaaagttc aatagtgtca ggttattaga ttttatggga
     7081 aaagagctta atttaaacac aaagtcaagt agcgctgaac tatatctaaa tattgaaaaa
     7141 atacccgtag gtgtttatat tttgctcatt gatgttttgc atcataataa catcctgttt
     7201 actaaaccca taaaatttat aaaaacatga agtatcaatt attagtaata gtgatcttca
     7261 ttttttttgt taatggcgca cgttcgcaat taataccgga aaaagccgcg gttgatacaa
     7321 gttatagtat ttcaactgaa gagtttgaaa aaatgaaaaa caacggaatc gttttaccgc
     7381 caataaagcc cgctgacgat cgctgggcaa atctacatta taagtcgagc agagaattac
     7441 ctttaatgta cgacatgcgt cagactccat ggctgacttc ggtaaaaaca caatcggcgg
     7501 gaggatgctg ggcatactcg gtgatgggaa ccgttgaagc aagattgctt atgcttgaca
     7561 aaggatatta tgacctttcg gataataacc tgaaaatttg ccataaatac ctgcccgaac
     7621 gaaatactta tggtaatcat tggatggcta cctcttattt cgctcgacat tcaggcccat
     7681 ttcttgaaag cgacgatcct tatccgggcg gcactactgc accggaaaat tgccctaatg
     7741 acttaactcc attattttat attggcaatt cacgttatcc gccgccgctt gataaattgt
     7801 ttattaaaca aactgttttg gagtacggcc cagtatggac attgatgtat tatcatgctg
     7861 atttttttaa accgactaat gctacatatt attacggggg gaacaagcag gttaatcatg
     7921 caggcgtaat tgtagggtgg aacgatactt tacaaaccga tggtggaagc ggcgcatgga
     7981 tcgttaaaaa tacctatggc ccaaattggg gagagggcgg atatttctat gtctcatttt
     8041 tcgattccca atttctaaaa tacaactctt attggcctga aataacagaa aatgaagata
     8101 gtacatatct atatcagtat gacgaagttg gaggatattg gggcgtcgga ttcggtaatg
     8161 aaataggtta tggtcttgta aaatttgaag caatagaagg gatttcggaa attatcaaag
     8221 tcggcacttt cgttcaatat gcagggtctg gaattgagat caaaatttat ggaaattttg
     8281 acggtcaaat aagcgacctg cttttatcta aagatgaagt aatatgcgag ctgccgggtt
     8341 actacacttt tgatctggat tctacaattt acatcccgca aggcaagtcg ttttacgttc
     8401 aggtaaaata caactccatg aaccctgaaa ataaatggcc tgtatctgtt gaggacacaa
     8461 ttatcaatta ttgtaacccc cagatcgaat ccggaaaatt ttggattgct cctaatcctg
     8521 atatctggcc tactgcatgg tatcaggtgg gcgctggaac agaatatcac tacgacctat
     8581 gtataaaagc atattcaaga caaattccaa tgcctccggc accaatagca gaagctggtg
     8641 aagatataac aataattgaa ggagaaagtg cgcagcccga aaatgctata gccgaaaatt
     8701 attcttccct attatggagt tccaatggag acggtgtttt gataaacgaa acaacgccta
     8761 atcctactta catccccggt cctcaggata tttataaagg aactgtcaat ttaatatttc
     8821 aggttattag tctgccgccg ctctatactg taaaaaccga tactctaaaa gtttttattt
     8881 taagataccc tactgtagag cttacctttc cacagcatgg ccaaaaactt tgtgatcctc
     8941 aaatcatcat tacgggaaat gcgtatgacc ctgacggcga tcttcaatcc gttgactaca
     9001 gcctaaacaa taatgaatgg ttaacatccg gaaatagtgg aacttggtcg gccacaatta
     9061 ctttatctcc cggagccaac acccttaaag caagagcaac agatgcttat gggctttcga
     9121 aagagactga ggaaatcaag gtaatatgta gtattcaaaa tatttttcta cctcaaggat
     9181 ggtcgctaat atctggtttt ttattgccag acgaaacaga gatagcaaaa atgtttgaca
     9241 gtgtagccag taatctcatc ctgcttcaaa ataccaacgg catatttgca cctccaccga
     9301 taaatataaa tacattgtcc aactgggata tccattctgg ctataaacta aaaatggctg
     9361 cggacgatga acttatcttt tgtggcaata tcccggataa taaccaatta gaattatcca
     9421 gtggctacaa actcataccc tatttaagta atcaacccgc aacaattgat cagctttttc
     9481 aaaatccggc aactgacata ctatatgttt ttgatctaac caactcattg atatattggc
     9541 ctgtgggtga aatttataca cttactaccc ttttccccgg aataggatat caggcgttct
     9601 ttatcaatca aaaaaactct ttcacaaatt gtaaaacaaa ccctcct
//
LOCUS       DAOOAI010000085         9375 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_9111_length_9375_cov_4.340236, whole genome shotgun sequence.
ACCESSION   DAOOAI010000085 DAOOAI010000000
VERSION     DAOOAI010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9375
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9111_length_9375_cov_4.340236"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..318
                     /locus_tag="PK345_06785"
     CDS             <1..318
                     /locus_tag="PK345_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34755.1"
                     /translation="IIKNNFGPTNTLRLGTEWNAGPYQIRGGYANYGSPFHLDVNDGM
                     IQSVSAGLGYRTKTYYIDLGVAYSFGNMDYYLYNADYIRTNPVDNRYETINFGFTYGY
                     RFN"
     gene            complement(407..1219)
                     /locus_tag="PK345_06790"
     CDS             complement(407..1219)
                     /locus_tag="PK345_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007569643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc ribbon domain-containing protein"
                     /protein_id="HPX34756.1"
                     /translation="MSSNIDQTKLDASAAEEENSEILELTIEKKLKALYSLQQIDSQI
                     DKIRIIRGELPLEVQDLEDEIAGLQIRIAKYKDDAKTLEKQISDKTNTIKDIKLLIKK
                     YEEQQMNVRNNREYDALTKEIEFQNLEIELEEKRIREFTHEFDTKNQLAKESRTVLKE
                     READLKSKKVELKDIVAETEKEENTLLKKSEMYSKMIEDRLLIAYQRIRKNARNGLAV
                     VQIERDACNGCFNKIPPQHQLDIKMHKKIIVCEYCGRILVDDNIANQVKLEL"
     gene            complement(1266..2363)
                     /locus_tag="PK345_06795"
     CDS             complement(1266..2363)
                     /locus_tag="PK345_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010538027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051540 - metal cluster binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HPX34757.1"
                     /translation="MKIADIIKVFDEAAPAELQESYDNAGLIIGSPDDEVKAALITLD
                     VTEEVIEEAINLKCDLIIAHHPLIFKGLKSLGRKNPVERMAITCIRNNIAVFAIHTNL
                     DNVLTGVNRRICDKLKVKNPVVLEPKRQQMRKLVTFCPADYTDKVRIAIFEAGAGHIG
                     DYDYCSFNVSGQGTFRGLEGATPFVGKVNELHFENEVRIETIFPVYRQRQIVEALLSA
                     HPYEEVAYDIYPVTNEYNIVGAGMIGSLDSPVETEVFFERIKNVFNAKSIRHTKLIFD
                     KIQRVAVCGGSGSFLLGSALARKADIFVTADVKYHDFFEADGKIIIADIGHFESEQFT
                     PELIKDILLEKIPTFATRLSQVNTNPVFYYL"
     gene            complement(2376..3122)
                     /locus_tag="PK345_06800"
     CDS             complement(2376..3122)
                     /locus_tag="PK345_06800"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HPX34758.1"
                     /translation="MSQDYKILHSYSVGSTNDLAEATLSREAVDEFTVFFADYQTNGK
                     GIGHNIWESNMGENLLISIVLHPVFLNPADQFMINKIVSLGVCKCLDKYITGQRITIK
                     WPNDVWVGFGKIAGILSRNLIMGNRFETCIVGIGLNINQQNFSPEIPNPVSMYQIAHR
                     AFDRNEVLRQLIDDVKHFYSLLKKNLIDTINQYYLERLLFYNTSATFIADDQEFTGII
                     KGVDEFGLLLVEVEENVRRYNIKDISLKVH"
     gene            3289..3687
                     /gene="rsfS"
                     /locus_tag="PK345_06805"
     CDS             3289..3687
                     /gene="rsfS"
                     /locus_tag="PK345_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008446690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HPX34759.1"
                     /translation="MIKNKISETKPHDKNVLINYIVAGIREIKGLDIVDVNISSIKNA
                     ICDNFIICHGTSRKHVDAIADSVEQMVWKKTGEKPWHKEGFQNAEWVLLDYVDVVVHI
                     FNDANRKFYNLEGLWADATINHFDPLTESK"
     gene            3816..5810
                     /gene="ftsH"
                     /locus_tag="PK345_06810"
     CDS             3816..5810
                     /gene="ftsH"
                     /locus_tag="PK345_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HPX34760.1"
                     /translation="MLIAAVFFALFFFNRNGETKETTWNDLRQMLIEEDIEKIVVVNN
                     EIAEIFIKQDKIGKQRYDKLRESVSNNFLAQNPHYKIVIGSVETFDKKIEEAQEGINN
                     PVYIEYARRRNWSGDIMSWVIPFLLIIGLWIFIMRMMSRGGPGGGQIFNIGKSKAQLF
                     DKDTHVTVTFKDVAGLEEAKIEVMEVVDFLKNPTKYTKLGGKIPKGALLIGPPGTGKT
                     LLAKSVAGEANVPFFSISGSDFVEMFVGVGASRVRDLFRQAKEKAPCIIFIDEIDAIG
                     RARGRSAITGANDERENTLNQLLTEMDGFSTNAGVIILAATNRADILDRALLRAGRFD
                     RQIHVELPDLDERKAIFLVHLKPVKIDDSVDVDFLSKQTPGFSGADIANVCNEAALIC
                     ARKGKKVVDKTDFMDAIDRIIGGLEKRNKIISQQEKRTVAYHEAGHAAISWLLEHASP
                     LVKVTIVPRGKALGAAWYLPEERQITTFEQMFDEMIATLGGRASEEVNFGVISTGALN
                     DLERVTKQAYAMVMYYGLNKKLGNISFYDSSGQQEYSFTKPYSEKTAQLIDEEVHKLI
                     EQAYNKAVNLLTENKSKVDKLAQLLLDKEVIFSEDLEKIFGKREFARDVEKRIEYEEE
                     KRRLREKLHHIKNENSKQEEIKKTVEKNDVDTKSEDQSNE"
     gene            5844..6500
                     /locus_tag="PK345_06815"
     CDS             5844..6500
                     /locus_tag="PK345_06815"
                     /inference="COORDINATES: protein motif:HMM:NF014628.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HPX34761.1"
                     /translation="MATAKEITAKENVYKNIRNALIDKMDNPFYGVDQDASVYNLLKE
                     SNDVTFAQEFTKVSGKFVYCESVDDLGKKLKYICFENKLQNIFCFEDDLKQLLDEYEI
                     AVCDDKTKILEVNTSISYCECLVARLGSVVVSSKQISGRRLIALPETHIIVAFTWQLV
                     EDLKDALAFLKEKYQKKLPSSITFITGPSRTADIEKTLVLGAHGPKELFVFLVEANDK
                     "
     gene            6518..6727
                     /locus_tag="PK345_06820"
     CDS             6518..6727
                     /locus_tag="PK345_06820"
                     /inference="COORDINATES: protein motif:HMM:NF020967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HPX34762.1"
                     /translation="MNNEWIVAYTSNNMYKVDLVKGLLLENGIKSEIMNKKDSVFLTG
                     EIELYVHPNDFKKAQRIISERKDIE"
     gene            6720..7544
                     /locus_tag="PK345_06825"
     CDS             6720..7544
                     /locus_tag="PK345_06825"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HPX34763.1"
                     /translation="MNNFLLRTITGLVYVVVIIGSLLLGAKVFASLFFIINILTLNEF
                     LTNIKRIRLPSLNLYFGIASGAVLYLLITLTGLGYLPPTLLSIALIIPLSLFARELFA
                     KNQSPITNIAISTLGLIYITIPFALLVTLYPTPQSSDPFPWNILTLFVIVWINDTFSY
                     LTGITFGRNLLFKRISPKKTWEGTIGGFLFSLAGGFVMYKISSGYSMILWIILSGLIS
                     ISALIGDLSESVIKRSLNIKDSGNLLPGHGGLLDRFDSLLFATPVVYVFITLLGKL"
     gene            7541..8197
                     /locus_tag="PK345_06830"
     CDS             7541..8197
                     /locus_tag="PK345_06830"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: protein motif:HMM:NF003678.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004609 - phosphatidylserine decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HPX34764.1"
                     /translation="MKIHREGIPIIIITILMITGIVLIINYYHPTQGAIHYVCYLALT
                     SLLVFIIRFFRNPNRPLTIDQSTVISPADGKIVEIAETDEPEFFGDKRLKISIFMSPN
                     NVHKNWYPISGTVRFFKHHPGKFLVAWHPKSSDLNERTTIVLENNREAKILVRQIAGA
                     VAQRIKCYAENTKTVNQGDELGFIKFGSRVDLFLPLGTEILVTQNQKVKGCQTVIAKL
                     "
     gene            8260..9177
                     /locus_tag="PK345_06835"
     CDS             8260..9177
                     /locus_tag="PK345_06835"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005940123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HPX34765.1"
                     /translation="MSFSITVLGSGAATPTIGRNPSGIFVNIGNNYFLIDCGEGTQMQ
                     LRRFNLKAQRINHVFISHLHGDHFFGLVGLISTFHLTGRKETLHIHSDPQLQEIIALQ
                     LKHTQTQLVYPLEFHPFSQPHETLFENEQVTVKSFPLVHKITSTGFLFSEKQKPRKIK
                     KDFIQHEEVSIKDIKRIKAGEDYVSPQGRVYLNRDITIAAPPPRQFAYCSDTRFDEKI
                     ADYIKNVNLLYHEASFLDDELDTAKEKFHSTASQAATIAAKAKADQLLLGHFSARYKD
                     LNGFMKEASKVFPNTVLAEDGLTITIPLK"
     gene            complement(9185..>9375)
                     /locus_tag="PK345_06840"
     CDS             complement(9185..>9375)
                     /locus_tag="PK345_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34766.1"
                     /translation="YLTSTPTSSATIESNMVAGRSRNTAVWRLLNTAIVMNFPYPTSA
                     LAFKLTLMTYTPSWNVSC"
BASE COUNT         3063 a         1793 c         1703 g         2816 t
ORIGIN      
        1 attataaaaa ataattttgg tcctaccaat actttacgat taggaacgga atggaatgcc
       61 ggaccgtatc aaataagggg tgggtatgcc aattatggaa gcccgtttca tttggatgta
      121 aacgatggta tgatacaatc agtttcggca ggattaggat atcgtacaaa aacttattat
      181 attgatttag gcgttgctta ttctttcggc aacatggatt actatcttta caatgctgat
      241 tacataagaa ccaaccctgt agataaccga tacgaaacta ttaattttgg ttttacttac
      301 ggatataggt tcaattaaac aatatcctga ctaagaaaac tatcaggata aaaaccagaa
      361 aaaacaaacc tttataaggt tttactatct ccttttacta gtttctttac aattccaatt
      421 ttacctgatt ggctatatta tcatccacaa gtattctccc gcaatattcg catacgatga
      481 tctttttatg cattttaata tcaagttggt gttgaggtgg aattttattg aaacacccat
      541 tacacgcatc tctctcaatc tgaactacgg caagaccatt ccgcgcattc tttcttatcc
      601 tttgataagc aataagcaga cgatcttcaa tcatcttact atacatttcc gatttcttca
      661 atagggtatt ttcctctttt tctgtttcag caactatatc tttcaattca acttttttgc
      721 tttttaaatc cgcctcacgc tctttcaata cggttcttga ctccttagca agctgatttt
      781 tcgtatcaaa ctcatgcgta aattcgcgaa ttcttttttc ctcaagctca atttcaagat
      841 tctgaaattc aatctccttt gtcaaagcat cgtattcacg gttatttcgt acattcatct
      901 gctgctcttc gtactttttt atcaatagct taatatcctt gatcgtattc gttttatcgg
      961 atatctgctt ttcgagcgtc ttagcatcat ctttgtactt cgcaatacgt atctgaagcc
     1021 ctgcaatttc atcttcaaga tcctgtactt caagcggcaa ttcacctcta ataattcgga
     1081 ttttatcaat ctgcgaatca atttgttgca gactgtaaag agcttttaat ttcttttcaa
     1141 tagtaagttc caggatttcg gagttttcct cctctgctgc tgaagcgtct aacttggttt
     1201 gatcaatgtt gctactcatt gaagttttta tttaattttc tgttaaaata gttatttaca
     1261 attaattaca aataataaaa aaccggattg gtatttacct gtgaaagtcg ggttgcaaaa
     1321 gtaggtattt tttccaacaa aatatcctta attagttccg gtgtaaattg ttcgctttcg
     1381 aaatgcccga tatcagcgat gataatcttt ccatcagcct caaaaaagtc atggtacttc
     1441 acatctgctg ttacaaaaat atcggctttt cgggctaagg cgcttccaag taaaaagctg
     1501 ccactacccc cacaaacggc aactcgttgt attttatcaa aaatcaattt agtatgccta
     1561 atagatttgg cattaaaaac attttttatg cgttcaaaaa aaacctcagt ttcaacgggc
     1621 gaatctaagc tgcctatcat gccagcgcca acaatattat attcatttgt aactgggtaa
     1681 atatcgtaag ccacttcctc ataaggatga gctgaaagca gcgcttctac tatctgcctt
     1741 tgcctgtaaa caggaaaaat agtctcaatt ctcacttcat tttcaaaatg caattcgtta
     1801 accttgccaa caaagggagt tgcgccttcc aaccctctaa aagtgccttg ccccgataca
     1861 ttaaaactac aataatcgta atcccctata tgccctgccc ctgcttcaaa aatagcaata
     1921 cggactttgt cggtataatc agccgggcaa aatgtaacta actttcgcat ttgttgcctc
     1981 tttggttcta aaacaacagg attttttact ttaagtttat cacaaatcct gcgattaaca
     2041 ccggtaagaa cattatcaag attagtatgg attgcaaaga cagcaatgtt atttcttata
     2101 caggtgattg ccatcctctc cacaggattt ttcctgccaa gactcttcaa tcctttgaag
     2161 attaacggat gatgagcaat aattaaatcg cattttaaat taatagcctc ctctattacc
     2221 tcttcagtaa catcgagggt tattaaagca gctttcactt catcgtcggg agagcctata
     2281 atcaggccgg cattatcata agattcctgc agctcggcag gcgcagcctc atcaaaaact
     2341 tttatgatat cagcaatttt catggtttta gtttattagt gcacttttaa ggagatatct
     2401 ttgatgttat accgccgtac attttcttcg acttcaacca ataaaagacc aaattcatca
     2461 acgcctttta ttattccggt aaactcttga tcatcagcga taaaagtagc tgaagtatta
     2521 taaaaaagta atctttccaa atagtattga ttaatagtat ctatcaaatt ttttttcaat
     2581 aaactgtaga aatgtttcac atcgtcaatc aactgcctca acacttcatt tctatcaaaa
     2641 gcccgatgag caatctgata catcgagaca ggatttggta tttccggact aaaattttgt
     2701 tggtttatgt taagaccaat acccactata cacgtttcga acctattacc cataatgaga
     2761 ttacgggata aaattcccgc aatttttcca aatcctaccc acacatcatt tggccattta
     2821 atagtgatac gttgcccggt aatatatttg tcaagacatt tacaaacgcc aagcgaaaca
     2881 attttattta tcataaactg atctgccgga ttaagaaaca caggatgcaa aacaatacta
     2941 atgagcaggt tttcacccat attactttcc caaatattgt gtcctatacc ctttccattt
     3001 gtttgataat cagcgaaaaa aacggtaaac tcatccacag cctctcgact taacgtagct
     3061 tcggccaaat cgttggttga gccaacagaa taactatgca atattttgta atcctgagac
     3121 atggatatta tttgaacata tgattcgaaa taaggttatt aaatagatga aacaacatag
     3181 tacagaaatc gttaccacaa ttctgaattt aaaattcaga attttgaatg aaatggttct
     3241 aacacgatgg agagcaaaat tagttacttt tgctgaaaat ttattttaat gattaaaaat
     3301 aagatatcag agactaaacc acacgataaa aacgtactta taaattacat cgtagccggc
     3361 attcgcgaga ttaaaggtct tgatattgtt gatgtcaaca tcagttcaat aaaaaacgcc
     3421 atttgcgata atttcattat ctgccatggt acatcacgga agcatgttga tgctatagca
     3481 gattctgtag aacaaatggt ttggaaaaaa accggagaga aaccttggca taaagagggt
     3541 tttcagaatg ccgaatgggt tttgcttgac tatgttgacg ttgtagttca tatttttaat
     3601 gatgctaacc gtaaatttta taatttagag gggctttggg ctgatgctac tattaatcat
     3661 tttgaccctt taaccgaatc aaaataataa ccgtgaacga aaacaaaaag aaagatactc
     3721 aaaaagaaag aaaggatgat ttgttgaaga acctgaacaa gggaggcaaa ccaggcgact
     3781 caaacaaagg gaaattcaat ttctattgga tatatatgtt gattgcagcc gttttttttg
     3841 cgctattttt tttcaatcgc aatggtgaga ccaaagaaac tacctggaac gaccttcgac
     3901 aaatgctgat agaagaggat attgaaaaga ttgtcgtagt aaacaacgaa attgctgaaa
     3961 tctttattaa acaggataaa attggcaagc agcgttatga taagctacgt gaaagtgttt
     4021 caaataactt ccttgctcaa aatcctcatt ataaaatcgt aatcggctca gtagaaactt
     4081 ttgataaaaa gattgaagag gctcaggagg gtatcaataa tcctgtttat attgaatatg
     4141 ccagaaggcg aaactggagt ggcgatatca tgagttgggt tatcccgttt ctcctaatca
     4201 tcggtttatg gatatttatt atgcgaatga tgagccgagg cgggcccgga ggcggacaga
     4261 tatttaatat cggtaaatcc aaagcgcaac tgtttgataa agatacgcat gtaactgtta
     4321 cttttaaaga tgtcgccgga cttgaagagg caaaaataga agtgatggag gttgtagatt
     4381 ttttaaaaaa cccaaccaaa tatactaaat taggaggaaa aatacctaag ggcgctttgt
     4441 tgatcggacc gccgggtacc ggaaagactc ttctggctaa atcggtagcc ggcgaagcca
     4501 acgtaccctt tttctcaatc tcaggaagcg acttcgtaga gatgttcgtc ggtgttggag
     4561 catcaagggt gcgcgatctt ttcaggcagg caaaagaaaa agctccctgc attattttta
     4621 tagatgaaat tgatgcaatc ggtcgcgcca gagggaggag cgccattaca ggtgctaacg
     4681 acgagcgcga gaatacatta aatcagttgc ttactgagat ggatggtttt agcaccaacg
     4741 ccggggtaat aatccttgct gctaccaata gggccgacat tcttgatcgt gccctgttac
     4801 gcgctggccg tttcgaccgt cagatacatg ttgagctacc cgatctggat gagcgcaaag
     4861 cgattttcct tgtgcatctt aagcctgtaa agattgatga tagtgttgat gttgattttc
     4921 tatcaaaaca aactccggga ttctccggcg ccgacattgc caacgtatgc aatgaggctg
     4981 ctctaatctg tgcaagaaaa ggaaaaaagg tagtagataa aaccgacttc atggatgcta
     5041 ttgacaggat tattggcgga cttgaaaagc gaaacaagat catctctcaa caagaaaaaa
     5101 gaaccgttgc ctaccacgag gcaggccatg cagcaataag ctggctactt gaacacgcat
     5161 cgccgttggt taaagtaact atcgtccctc gtggcaaagc ccttggagca gcatggtatc
     5221 tgccagaaga gcgacaaata actacatttg aacaaatgtt cgacgaaatg attgcaaccc
     5281 tcggcggaag agcctccgaa gaggtaaact tcggagtaat ttcaacaggc gcactgaacg
     5341 acctggaaag agttacaaaa caagcttacg caatggttat gtattatggg ctgaataaaa
     5401 aactcggaaa catcagcttc tacgactcta gcggacaaca agagtactca ttcacaaaac
     5461 catatagcga aaaaacagcc caacttattg acgaagaggt acataaactt attgaacaag
     5521 catataataa agcagtcaac cttcttaccg aaaataaaag caaggtggac aaattagcac
     5581 aactactatt agataaagag gtaatattta gtgaagacct tgaaaaaatc tttggaaaaa
     5641 gagaatttgc aagagacgta gaaaagagaa ttgaatacga agaagagaaa agaagactta
     5701 gagaaaagct acatcatatc aaaaacgaaa actcaaaaca ggaagagata aaaaaaacgg
     5761 tggaaaagaa tgatgtagat accaaatcgg aagatcagtc aaatgaataa aagaacgttg
     5821 tttaaaaaat aatttatttg gcaatggcca ctgcaaaaga aataactgca aaagagaatg
     5881 tttataagaa tattcgcaat gccctaattg acaaaatgga taatcccttt tacggagttg
     5941 atcaggatgc ttcggtttac aatttgctca aggagagtaa tgacgtaact tttgcacagg
     6001 agtttacaaa ggtttccggt aaatttgtct attgcgaaag cgttgatgac cttggaaaaa
     6061 aactaaagta tatctgtttt gaaaataaac ttcaaaatat tttctgtttt gaagatgatt
     6121 taaaacaact gctcgatgaa tatgaaattg cagtttgtga tgacaagacc aagatacttg
     6181 aggtgaatac aagtattagt tattgcgaat gcctagtagc ccgacttgga agcgtagttg
     6241 tttcttcgaa acaaatatcc ggaagacgtc taatagcatt acccgaaacc catattattg
     6301 tggccttcac ttggcagctt gtcgaagatt taaaagacgc cttagctttt ttaaaagaaa
     6361 aatatcaaaa aaaactgccc tcatcaatta ccttcattac cggcccaagc cgtactgccg
     6421 atatcgaaaa aacattagta ttgggggcac atggcccaaa agaattattt gtatttcttg
     6481 ttgaagcaaa cgataagtaa ctataaaaca aatgactatg aataatgaat ggatagtggc
     6541 ctatacatct aataatatgt ataaagttga cttagtaaaa ggactactgc tcgaaaacgg
     6601 tattaaaagt gagataatga ataaaaagga tagtgtattt ttaactggtg aaattgaact
     6661 ttatgttcat cccaacgatt ttaaaaaagc acaaagaata atctctgaac gtaaagacat
     6721 tgaataattt tctgttgcgc actattaccg gtttagtata tgtagttgtt attatcggtt
     6781 ccttgttatt gggagccaaa gtatttgcat ccctattttt tatcattaac atactgactc
     6841 ttaatgagtt tctgacaaat atcaaaagga ttcggttacc ctcgcttaat ttgtattttg
     6901 gaatagcatc cggtgcagtt ctttaccttc taatcacttt aacagggtta gggtatttac
     6961 caccaacatt gttatctatt gcattaataa tccctttgtc gctatttgcc agagaattat
     7021 tcgccaaaaa ccagtcgcca atcacaaata tcgccatttc tactttaggt ttaatttaca
     7081 taactattcc ttttgcactt ttagttacac tttaccctac cccacaatca tccgatccat
     7141 ttccatggaa tattttgacg ctctttgtca ttgtatggat taacgataca ttttcatatt
     7201 taaccggaat aactttcggg cgaaacttat tgtttaagcg tatttcacca aaaaagacat
     7261 gggaaggaac tattggcgga tttctatttt cacttgccgg tggatttgtc atgtacaaaa
     7321 tcagtagcgg gtattcaatg attttatgga tcatactatc agggttaata tctatatcgg
     7381 cgctcattgg cgatctttcc gaatcagtga taaagcgtag tttaaacata aaagatagtg
     7441 gaaatctttt gccaggtcat ggtggcttgc tcgacaggtt cgattcactt ttgtttgcca
     7501 caccggttgt atatgttttt ataactttgc tcggtaaatt atgaaaatac atagagaagg
     7561 aataccgatc attatcatta ctatattaat gattacaggg attgtgttaa tcatcaacta
     7621 ttaccatccc acccaaggag ccatacatta tgtttgttat ctggcactta cctcattact
     7681 cgtttttatt attaggtttt ttcgcaaccc aaaccgcccg cttactattg accaatccac
     7741 agtaatctcg ccggctgacg gtaagattgt ggagatcgca gagactgatg aacctgagtt
     7801 ttttggagac aagcgtttaa aaatctccat ttttatgtcg ccaaacaatg ttcataaaaa
     7861 ctggtatccc attagtggta ctgttcggtt tttcaagcat catcccggca aatttttggt
     7921 tgcctggcat cccaagtctt cggatctgaa cgaaagaaca actattgttt tagaaaataa
     7981 cagagaagct aaaatccttg taaggcagat tgctggcgca gttgcacaaa gaataaaatg
     8041 ttacgcagaa aacaccaaaa ccgtcaatca aggtgacgaa ctgggtttca tcaaatttgg
     8101 atcaagagtt gaccttttcc tacctctggg aactgaaata cttgtaacac aaaatcaaaa
     8161 agtaaaaggc tgccaaacgg ttattgcaaa actataagac ggatttataa taatgaagcc
     8221 ctgttgcgaa aagatattta atagcttcaa tgatttaata tgtcattttc aattacagtt
     8281 ttaggaagtg gagcagcaac cccaactatt ggccgtaatc cctcaggaat atttgtaaat
     8341 atcggcaaca attatttcct gattgattgt ggtgaaggaa ctcaaatgca gcttcgccga
     8401 tttaatctta aagcgcaaag aatcaatcat gtattcatca gccacctaca tggcgatcac
     8461 ttttttggtc tggtagggct tatctcaaca tttcatttaa caggtcgaaa agagacacta
     8521 cacattcata gcgatccaca gttacaggaa attattgcgc tgcaattgaa acatactcaa
     8581 acccaattgg tctatccact cgaatttcat ccatttagcc aaccgcacga aacacttttc
     8641 gaaaatgaac aggtaactgt taaatcattt cctttagtac acaagatcac ctctacagga
     8701 tttctgtttt cagaaaaaca gaaaccaaga aaaataaaga aggattttat acagcatgaa
     8761 gaggttagta taaaggatat taaaagaata aaagccggtg aagattatgt ttcgccgcaa
     8821 gggagggttt atctcaatcg cgatattacc attgccgctc cgccgccacg ccagtttgcc
     8881 tactgttccg acacacggtt cgacgaaaag attgctgatt atatcaaaaa tgtaaactta
     8941 ctttaccatg aagcatcttt tttggatgat gaattggaca ccgccaaaga aaaatttcat
     9001 tcaacagcaa gtcaagcagc aacaatagct gcaaaagcaa aagctgatca attgcttctt
     9061 ggtcattttt ctgctcgtta taaagattta aatgggttta tgaaagaggc atccaaagta
     9121 tttcctaaca cagtattagc cgaggatggc ttaaccatta ccatcccatt aaaatagcca
     9181 acccttaaca actcacattc cacgaaggag tataagtcat cagtgtaagt ttaaaagcca
     9241 aagcggatgt gggataagga aaattcataa caatggcagt atttaacaag cgccaaactg
     9301 ctgtgtttcg gcttcgcccg gcaaccatgt tactttcgat ggttgccgaa cttgtcggag
     9361 tactggttaa gtaac
//
LOCUS       DAOOAI010000086         9333 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_9156_length_9333_cov_4.967881, whole genome shotgun sequence.
ACCESSION   DAOOAI010000086 DAOOAI010000000
VERSION     DAOOAI010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9333)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9333)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9333
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9156_length_9333_cov_4.967881"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..861)
                     /locus_tag="PK345_06845"
     CDS             complement(<1..861)
                     /locus_tag="PK345_06845"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPX34767.1"
                     /translation="MEKVKQTLRDSKTARWIALALISLTMFFAYFFIDVAAPLQIMFQ
                     TDYGWSPEVFGMLGGSEFFLNVFAFFLILSGIILDKMGIRFTIITSGVTMVIGGSIKY
                     FALSKGFVGSGIEQFLSSFLVNIPASAKLAFFGFAIFGVGVEMAGITVSKTIVKWFKG
                     KELALAMGLEMAIARLGVFAVFRLSPIFAENGGASNSVLWGVAFLIVGLLLFLIYTIM
                     DVRLDRQLGEEAEIELEDAFRISDLWLILKNPGFLAIASLCVLFYSAIFPFQKFATDM
                     LSSKLDISIKE"
     gene            1012..2793
                     /locus_tag="PK345_06850"
     CDS             1012..2793
                     /locus_tag="PK345_06850"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPX34768.1"
                     /translation="MAKDNILKGGEFLIKETKAADVFIPEDFSEEQVMIAQTCNDFLE
                     SFVYPNLDRIDNQEEGLMKHLLQKSGELGLLAISIPSEYEGFDQSFVTSMIASEYMGA
                     GYSFSVAYSAHTGIGSLPITYYGNDFQKQKYLPSLGSGALTSAYCLTEPNAGSDANAG
                     KTKAVLSEDGKHYILNGQKMWITNGGFADVMVVFAKIDNDRVLSAFIVERAYPGIVFN
                     PEEKKMGIKGSSTVQIFFNDCKVPVENLLGNRGEGFRIALNILHMGRIKLGGTVLGAA
                     KRAITQSVNYANERRQFGKQIAEFGAIKYKLAEQVIKTWVTESSVYRVGQCIDDTIEK
                     LKEEGLPKGKANIEGIADYAIEAAFLKVFGSEALSYIVDEAVQIFGGMGFSSETSVER
                     AYRDSRINRIFEGTNEINRMLVIDTTIKRALKEGFDVKEYSKSILKELEEEQSDIPQC
                     YYAEKHYYVKNFKKAALLLLGEILEVFQRNLTNEQEILFNISDIIMQAYASESALLRL
                     EKLEGIKGAEAVTIYRDIVDVFIYDAADIIQKCGKDAINSFVDEEEHRIKLIDHINYL
                     AKVKPVNVKEARRRIANKLIEDNFYNF"
     gene            3132..4469
                     /gene="gdhA"
                     /locus_tag="PK345_06855"
     CDS             3132..4469
                     /gene="gdhA"
                     /locus_tag="PK345_06855"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008650340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HPX34769.1"
                     /translation="MDLKKFMNDLEKKHPGEVEYLQAVKEVLETLEDVLKENPQFEAA
                     GILQRIVEPDRILIFKVPWVDDNGKVHVNLAYRVQFNMAIGPYKGGMRFHPSVNLSIL
                     KFLGFEQTFKNSLTTLPMGGGKGGSDFDPKGKSDNEVMRFCQSLMLELWRIIGPETDV
                     PAGDIGVGAREVGYMYGMYKKLAQEHTGVFTGKGLNWGGSLIRPEATGFGAVYFTQEM
                     LATQGKSFKGKTVCISGFGNVAWGAVRKATELGAKVVAISGPDGYVYDPDGIAGEKIN
                     YMRELRNSNQDIVEPYATKFPGAKFVKGRRPWEVKCDIAMPCATQNELNKEDAENLMK
                     NGCWCVAEGANMPCTPEAIAVFQNARILFAPGKAVNAGGVSTSGLEMSQNALKLNWTS
                     EEVDARLQQIMKNIHQACVSDGKEKDGHVNYVKGANVAGFMKVANAMMDQGVL"
     gene            4744..6228
                     /gene="malQ"
                     /locus_tag="PK345_06860"
     CDS             4744..6228
                     /gene="malQ"
                     /locus_tag="PK345_06860"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HPX34770.1"
                     /translation="MKIERSSGILLHPTSLPGKYGIGSLGKSAFGFVDFLHKAGQKLW
                     QVLPMGHTGYGDSPYQCFSVFAGNPIIIDIDLLFKDGFLKRRDFLEMPTFSEEKVNYG
                     KVIYFKEKMLKLAFQRFDEKKDYNTVDYQVFVNNNQEWLKEYAIFIALKDRFGGKPWW
                     EWPEEFKLRQPDVLEKFIASSKNEISYHQFVQYHFYKQWSELKRYANKKNIKIIGDIP
                     LYVAHDSSDVWSNHQLFQFTSDRQPIAVAGVPPDYFSQTGQLWGNPLYNWDYMKKDNF
                     NWWVERVKSSFKLFDYVRIDHFRGLESYWSVPFGEKTAINGKWVKAPGDQLFEAIRNK
                     LGDLPIIAEDLGVITPEVDNLRLKYDFPGMKILQFAFHSDDGQKYLPHNYSENFIVYT
                     GTHDNDTLAGWFRSLDKSVRRRVLFYTDANTKTINRKMIRLAWSSVAKLAVIPLQDLL
                     ELGTEARMNVPGTLGGNWQWRLKKHQLKRKHSAWLAKITKIYNR"
     gene            6232..7971
                     /locus_tag="PK345_06865"
     CDS             6232..7971
                     /locus_tag="PK345_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family protein"
                     /protein_id="HPX34771.1"
                     /translation="MKTKISIYQTFPRLFGNKNKNPKIGGSIKENGVGKFNDYTSKAL
                     LTIRDLGITHIWYVGIIEHGTTTDYSKYGIAKDHPALVKGKAGSPYAIKDYYDVNPDL
                     AVDVKNRMTEFESLVQRTHEAGLKVIIDIVANHIFRQYHSDVKPEGVEDFGQNDDTTK
                     AFDPQNNFYYLPQTSFELPDGVLSNIAAIKTAYKPYIEVPARITGNDVFAAKPDIEDW
                     YETAKLNYGVDLQGNGDKCFNPVPDTWKKMTDIMLFWASKGVDGFRCDMAEMIPVEFW
                     NYAVNIVKKHYPDLIFIGETYNPLEYYNYINFGGFDFLYDKVGLYDTLKNIIIHNAST
                     LSISNCWKVLEGLDARMLRFIENHDEVRLASMAFAGDAKVAIPAMTLVATMNRGPVMI
                     YGGQEVGEPAQGESGFSGDDGRTTIYDYFAMPEHQKWMNNGKFDGGGLSADQTLLRQF
                     YQKILNFSLRYDAIANGEFYDLMWANPYGSLPQRNKIYIWLRHTSKQKLLFVISFDKS
                     HDQQIRIKISDHAFNEMGWWKCCDFEAKELLWNMQKLQFDRKTAIETGLIIDLRPNDA
                     LIFEIVPKDCREE"
     gene            8119..9129
                     /gene="amrS"
                     /locus_tag="PK345_06870"
     CDS             8119..9129
                     /gene="amrS"
                     /locus_tag="PK345_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR04337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HPX34772.1"
                     /translation="MMKTARYQESIQRYLSQCNLCPHHCVIGDGKTGRCRVRENTKGE
                     INLLTYGIVSSMGFDPIEKKPLYHFYPGKDIFSIGSFGCTLKCRFCQNWEISQDLPNH
                     YSRMKRFAPEELVMTAASKKGNIGIAYTYNEPTVWFEFMLDIAAIARKYKMKNVMITN
                     GFINPEPMDELLTFIDAFNVDLKAFTEEFYRTLTFSSLEPVKETLLRIKKAGKHLEIT
                     NLVIPELNDQTSDFTEMIHWISTCLGGDTPLHLSRYFPNYKLNQSATTAEDLKRLYNI
                     AKTKLKYVYIGNLAGNGSQNTECPSCNSTVIYRNRYNVLRSGLQKNGTCKNCGEKISG
                     FF"
BASE COUNT         3071 a         1674 c         1932 g         2656 t
ORIGIN      
        1 ctcctttata ctaatatcga gtttggagga aagcatgtct gttgcaaatt tctgaaatgg
       61 gaaaatagcc gaatagaata aaacacaaag gcttgcaatg gcgaggaatc ctggattttt
      121 taagattagc cataggtcgc tgatacgaaa agcatcttcg agttcaattt ctgcttcttc
      181 gccaagttgt ctatcaaggc gtacatccat aatagtatag atcaaaaaca agagtaatcc
      241 tactatcaaa aaggctacgc cccataagac cgagttggat gctccgccgt tttcagcaaa
      301 tatcggtgac aatctgaaca cggcaaaaac accaaggcga gctattgcca tctctaatcc
      361 cattgcaagg gcaagttctt tgcctttaaa ccacttcaca atggttttcg aaacagttat
      421 tccagccatt tctacgccta cgccgaatat cgcaaaacca aaaaaagcaa gctttgccga
      481 agcaggtata tttaccaaaa atgaacttaa aaactgctct attcccgagc ctacaaaacc
      541 tttggagagt gcaaaatatt tgatagaccc accaataacc atcgttactc cggatgtaat
      601 aatggtaaac cgtatgccca ttttatcaag aataatccct gataaaatca gaaaaaaagc
      661 aaacacattc agaaaaaact ccgatccacc taacattcca aatacttcag gcgaccatcc
      721 ataatcggtt tgaaacatga tttgaagcgg cgcggcaacg tcaataaaaa agtatgcaaa
      781 aaacattgtc aaagagatca gcgccaatgc gatccaacgt gcagttttag aatcgcgtag
      841 agtttgttta actttttcca ttataaagtg atttttttaa ttaaaattat ttttaaaaac
      901 cgccaaaagt aacatattta aaaaacttca cttaaaagaa aaataaaata gtaaacactc
      961 acaaataatt tagcttctta tctttgcagg cgaattttaa aaaattatta aatggcaaaa
     1021 gacaatattt taaaaggcgg tgaatttctg attaaagaga ccaaagctgc ggatgtgttt
     1081 atccctgagg atttttcaga agagcaggtc atgattgctc aaacctgcaa cgattttctt
     1141 gaatcgtttg tttatccaaa tctcgatcgt attgataatc aggaagaggg gctgatgaag
     1201 cacctgcttc aaaagtccgg cgagttaggg ctgttagcaa tctctatccc ttcggaatac
     1261 gaaggttttg atcaatcttt tgtaacttca atgattgcca gcgaatatat gggggccggc
     1321 tactcctttt ccgtggctta ttcggcacac actgggatcg gttctttacc aattacctac
     1381 tacggcaacg atttccagaa acaaaaatac cttcctagcc tcggtagcgg agcgcttact
     1441 tcggcatatt gccttacaga gccaaacgcc ggctcggatg caaacgctgg aaagacaaaa
     1501 gcagtcctct cagaagatgg caaacattat atcctcaatg gccaaaagat gtggatcaca
     1561 aacggtggat ttgctgatgt gatggttgtt tttgctaaga ttgataacga tcgtgtgctt
     1621 agcgctttca ttgtcgaacg tgcttatccc gggattgtat ttaatccgga agagaaaaaa
     1681 atgggcataa aaggttcgtc cacagtgcag atttttttca acgattgtaa agtgcctgtt
     1741 gagaatcttt taggaaatcg cggcgaggga tttcgtattg ctttgaatat tcttcacatg
     1801 ggtaggatta aacttggcgg tacggtactc ggcgctgcta aaagagctat aactcagtcg
     1861 gttaattatg ctaacgagcg ccgtcaattc ggaaaacaga tcgccgaatt tggagcaatc
     1921 aaatataaat tggccgagca ggttataaaa acatgggtta ccgaatcctc tgtatatcgt
     1981 gttggccaat gtattgatga taccatagaa aaacttaaag aagagggatt gccaaaagga
     2041 aaggccaata tcgaaggtat tgccgattac gctatcgaag cagcttttct taaagtgttt
     2101 ggcagcgaag cccttagcta catcgttgat gaagcggttc aaatttttgg aggaatgggt
     2161 ttttcgtcgg agacatccgt tgaacgtgca tatcgtgatt cgaggataaa cagaattttc
     2221 gaaggaacaa atgagataaa ccgcatgtta gttatagaca caaccattaa aagagcgctt
     2281 aaagaaggat ttgacgtaaa agaatattca aaatccatac ttaaagaatt agaagaagaa
     2341 caaagcgata ttccacaatg ttattatgcc gaaaagcatt attatgtgaa aaacttcaaa
     2401 aaagctgcgc ttttgctact tggcgaaatt ttagaagtgt tccagcgcaa tcttacaaac
     2461 gaacaggaga ttctgtttaa catttcggat atcattatgc aggcttatgc ttccgaatcg
     2521 gccttactta ggttagaaaa gcttgaaggg ataaagggag ctgaagcagt tacaatatat
     2581 agagatattg tggatgtttt catttatgat gccgccgata ttatccaaaa atgtggtaaa
     2641 gatgctataa actcctttgt tgacgaagaa gaacatcgca ttaaactaat agatcatatt
     2701 aactatttgg caaaagtaaa gcctgtcaat gttaaagaag cgcgacgccg gattgctaac
     2761 aagctgattg aagataattt ttataatttt taatagacaa atcaacgatt cagttttccg
     2821 ttttgttaac tcgaattaca tgattgttag gcccgaaaga aatttctatt ggtttcatac
     2881 agtgtatatg gctgtaaaag tctaattttt atttcatatt atcagattaa acagccatta
     2941 taacggttta aacattccga aataaaataa agaaggggct cttctcttta cttttactct
     3001 taccaaacct aacaatggga aaatattcgt gaaaagtttt ggcaaataga cttaaaagta
     3061 tgtactttgc atattaaaat taacactgtt aattatttca caattagttt aaactttaaa
     3121 aaattagtgc aatggatctc aaaaaattca tgaacgacct ggagaaaaag catccgggtg
     3181 aagtagagta tcttcaggca gttaaagaag tgcttgagac tctcgaagat gttttgaagg
     3241 aaaaccctca attcgaagct gctggaattc ttcagcgcat tgttgagccc gaccgtattt
     3301 taatatttaa agtcccttgg gttgacgata atggtaaagt acatgttaat ctggcctatc
     3361 gcgtacaatt caacatggct atcggcccat ataaaggcgg catgcgcttt catccaagcg
     3421 taaaccttag cattcttaaa tttttaggat ttgagcagac tttcaaaaat agccttacta
     3481 cattgcctat gggtggtgga aagggcggct ctgacttcga tccgaaagga aagtcggaca
     3541 atgaggtcat gcgcttttgc cagtctctca tgctcgaatt atggagaatc attggccctg
     3601 agacagacgt tcccgccgga gatatcggcg taggcgctcg tgaggtaggt tatatgtacg
     3661 ggatgtacaa aaaactggct caagagcata ctggcgtatt taccggaaaa ggtctcaact
     3721 ggggtggaag ccttattcgt cctgaagcaa caggattcgg tgcagtatat tttacacagg
     3781 aaatgcttgc aacccaaggc aaatcattca aaggaaagac agtttgtatc tctggctttg
     3841 gcaatgtagc atggggcgct gtaagaaaag ctaccgaact cggcgctaaa gtagttgcta
     3901 tctctggccc cgacggatat gtttacgatc cggatggcat cgcaggcgaa aagattaatt
     3961 atatgcgtga actgagaaac tctaatcagg acattgtaga accttatgca actaagttcc
     4021 cgggagcaaa atttgtaaaa ggaagacgcc catgggaagt aaaatgcgat atcgcaatgc
     4081 catgtgcaac ccaaaatgag ctgaataaag aggatgctga aaatcttatg aagaatggct
     4141 gctggtgtgt agccgaaggc gctaatatgc cttgtacacc agaggctatt gcagttttcc
     4201 aaaacgcaag aatcctcttt gcaccgggta aagccgttaa tgctggtgga gtatctacat
     4261 caggcctcga aatgtcgcaa aatgccctta aacttaactg gaccagcgaa gaggtagatg
     4321 caagacttca acaaattatg aagaatattc atcaggcatg tgtatccgat ggtaaagaaa
     4381 aggacggtca tgtcaattac gtaaaaggcg caaacgtggc cggcttcatg aaagtagcca
     4441 atgccatgat ggatcaaggc gtcttataat tgttagacat tattaaaaaa aaagcctctg
     4501 cctttgggtt agaggctttt ttttgtcttt ttaaatcatt acttcagctt gagaaaaaat
     4561 aaactcagct tattcatcag ttcgcttgta aattgttgat caatcgaaaa aaatgtattt
     4621 ggctcctctt agaaaaatag cttaatcaaa ttcacttaaa atagccaatt tttaaacctc
     4681 tttattttgt caattaccta caaagtcctt actttgcaga acgattaaaa aaaagtttta
     4741 gcaatgaaaa tcgaaaggag cagcggaatt ttattgcacc ccacctcact tcccggaaaa
     4801 tatggaatag gatcgttagg gaaaagtgca ttcgggtttg tggatttttt acacaaagca
     4861 gggcaaaagc tatggcaagt ccttccgatg gggcatactg gatatggaga ttcgccctat
     4921 caatgttttt ctgtgtttgc cggcaatcca attataatag atattgacct cttgttcaag
     4981 gatggttttt taaagaggag agattttttg gaaatgccta cattctcgga ggagaaggta
     5041 aactacggta aggtgattta tttcaaagag aaaatgttaa agcttgcctt tcagcggttt
     5101 gatgaaaaaa aggattataa tactgttgat tatcaggtgt ttgtaaataa taatcaagag
     5161 tggctaaagg agtatgcaat ttttattgcg ttaaaagaca ggttcggcgg caagccttgg
     5221 tgggaatggc ctgaagagtt taagctaaga caacccgatg tgcttgagaa gtttattgct
     5281 agttcgaaaa atgaaattag ctaccatcag ttcgtacaat atcatttcta taaacaatgg
     5341 agtgaattaa agagatatgc caacaaaaag aatattaaaa taattggaga cattcctctt
     5401 tatgttgccc atgatagctc cgatgtttgg agtaatcacc agctttttca gtttacatcc
     5461 gatcgtcaac ctattgctgt agcgggtgtt cctcctgatt atttcagcca aaccggccag
     5521 ttatggggca acccgcttta taattgggat tacatgaaaa aggacaattt caactggtgg
     5581 gttgaaagag taaaatcatc atttaagctc tttgattatg tgaggattga tcatttcagg
     5641 gggttggaat cctactggtc agttcctttt ggcgaaaaga cagccatcaa tggtaaatgg
     5701 gtaaaggcgc caggcgatca gttgtttgaa gcgatacgta ataagcttgg cgatttgccg
     5761 ataattgcag aagaccttgg cgttatcact cctgaagtgg ataatttgag gttaaaatat
     5821 gatttccccg gtatgaagat cttgcagttt gcttttcact ctgatgatgg tcaaaagtat
     5881 ctaccgcata attactccga aaacttcatt gtttatacag gaactcacga taacgacacg
     5941 ttggcaggat ggttccgaag ccttgataaa agcgttcgcc gtagagtact tttttatacc
     6001 gatgccaata cgaagacaat taatagaaaa atgatacgat tagcgtggag ctcggtagcc
     6061 aaattggcgg taatacctct tcaagatttg ctcgaacttg gcacagaagc aaggatgaat
     6121 gtgccgggta cgcttggggg aaactggcaa tggcggctta aaaagcacca attgaaaaga
     6181 aaacatagcg cttggttagc aaaaattaca aaaatttata accgatagat tatgaaaacg
     6241 aaaatctcta tctaccaaac atttcctcgg ctttttggaa ataaaaacaa aaatccaaaa
     6301 attgggggat ccataaaaga aaacggggta gggaagttta atgattatac ctcaaaagcc
     6361 ctattaacta tcagggattt aggaattaca catatttggt acgtgggtat aattgaacat
     6421 ggcactacga ctgactactc aaaatatggt attgctaaag atcatccagc gttagttaaa
     6481 ggaaaagcag gatcgccgta tgctatcaaa gattattacg atgttaatcc tgatttagct
     6541 gtggatgtta aaaaccgaat gacagagttt gaatcactcg ttcaacgtac gcatgaggca
     6601 ggattaaaag ttattattga cattgtagct aatcacattt tcaggcaata tcattcggat
     6661 gttaagcctg aaggagttga ggacttcggt caaaatgacg acacgacaaa ggctttcgat
     6721 cctcaaaaca atttttatta ccttccgcaa acatcttttg aactacctga cggtgtgtta
     6781 tcaaatattg ctgccataaa aacagcttat aaaccttaca tcgaagtacc agcgcgtatt
     6841 accggcaacg acgtttttgc cgccaagccg gatattgagg attggtacga aacagcaaaa
     6901 cttaattatg gcgtggattt gcaaggaaat ggcgataaat gttttaatcc cgttcccgat
     6961 acatggaaaa agatgaccga tattatgcta ttctgggcaa gtaaaggcgt agatggattt
     7021 cggtgcgata tggctgaaat gattcctgta gaattttgga attatgccgt aaacattgta
     7081 aaaaagcatt atccggatct tatatttatt ggcgaaacat acaatcctct tgaatactac
     7141 aattacataa attttggagg attcgatttt ctttacgata aagttgggct ttacgatact
     7201 ttaaagaata tcattatcca taacgcctcc actttaagta tttccaattg ctggaaagta
     7261 ttggaaggac ttgatgcacg tatgttgcgt tttatagaga atcatgatga agttcgtctt
     7321 gcttcaatgg catttgcagg cgatgctaaa gtagccattc ccgctatgac gttagttgct
     7381 acgatgaatc gcggacctgt tatgatttac ggaggtcagg aggttggcga gccggcgcaa
     7441 ggcgaaagtg gatttagtgg tgatgatgga agaacaacca tatacgatta ttttgctatg
     7501 cccgaacatc aaaaatggat gaacaacgga aaatttgacg gtggaggatt aagcgctgac
     7561 caaacgcttc ttcgacagtt ttatcaaaaa atccttaatt tcagcctgcg ttacgatgct
     7621 atcgcaaatg gtgaatttta cgatctgatg tgggcaaatc cttacggttc attacctcaa
     7681 agaaataaaa tctatatctg gttaaggcat acatcgaaac agaaactact ttttgtaatc
     7741 agttttgaca aaagccatga tcagcagatc agaataaaaa tttcggatca tgccttcaac
     7801 gaaatgggat ggtggaaatg ttgtgatttt gaagctaaag agttactttg gaatatgcaa
     7861 aaactgcaat tcgaccgaaa aacagctatt gaaacaggcc ttataataga ccttaggcct
     7921 aatgatgctt tgatattcga aatcgtacca aaagactgta gggaagaata aatctctaac
     7981 ctatctcttt tcgaaaaggt aacaacaaac ctacattgat gtatgattct gaaaataaat
     8041 gataaatttg gtaattttga gataaaagaa tatttaatta cgagcaattg catagtgtaa
     8101 taataaaagg cttggaagat gatgaaaaca gcccgatatc aggaaagtat ccaacgttac
     8161 ctatcacaat gtaacttgtg tccgcaccat tgtgtaatag gtgatgggaa aacaggtcgt
     8221 tgccgtgtta gagaaaacac aaaaggcgag atcaacctac ttacttatgg aattgtctct
     8281 tcaatggggt ttgatccgat tgaaaaaaag ccgttatatc atttttatcc aggaaaggat
     8341 attttttcaa tcggcagctt tggttgcacg ttgaaatgtc gtttttgtca gaactgggaa
     8401 atttcgcaag accttcccaa tcactattct cggatgaaac gttttgcccc cgaagagttg
     8461 gtaatgacgg cagcaagtaa aaaaggaaat attggtatcg cttataccta caatgagcct
     8521 actgtttggt ttgagtttat gctcgacata gccgctatcg cccgaaaata taaaatgaaa
     8581 aatgtaatga taacaaacgg ctttatcaat cccgaaccaa tggatgagct tctgaccttt
     8641 attgatgctt ttaatgttga cttaaaagct tttactgaag agttttatcg tacacttacc
     8701 ttctcctcac tcgaacctgt aaaagagaca ttattacgta taaaaaaggc gggtaaacat
     8761 ttagagataa caaaccttgt aattccagag cttaatgatc aaacaagtga ttttactgaa
     8821 atgatccatt ggatttctac ctgtcttgga ggtgatactc ccctgcatct gtcgcgttat
     8881 ttccctaatt acaaactaaa ccagtctgcc accacggcag aagacctgaa acgactttat
     8941 aacatagcca aaaccaaact caaatatgtt tatatcggaa atttggccgg aaacggaagc
     9001 caaaatactg aatgtccatc gtgcaactct acggttatat acagaaaccg ttataacgta
     9061 ttaaggagcg gcttgcaaaa aaatggaact tgtaaaaatt gtggagagaa gatttcagga
     9121 ttcttttaaa tcatttgata atcctataat tttcgaagag atttgccaaa atatcaaaaa
     9181 taacaccatt gtcaagatgt ttaactaaaa tgggcaatcc ttaaaaacat gtttaagtcc
     9241 tgtttataaa aaataaactg ttgataaaaa attgattttg agtgttccgc attgcaaagt
     9301 tggggctgta tcaaaataaa tatgtggcta aaa
//
LOCUS       DAOOAI010000087         9301 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_9189_length_9301_cov_6.140818, whole genome shotgun sequence.
ACCESSION   DAOOAI010000087 DAOOAI010000000
VERSION     DAOOAI010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9301)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9189_length_9301_cov_6.140818"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="PK345_06875"
     CDS             complement(<1..234)
                     /locus_tag="PK345_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34773.1"
                     /translation="MKNILSALLILFAINAYTQIPEIVWQKCYGSPESDGSYGIVSKG
                     DGLLIAIHLLDSIPGATNYHGKGDIWIINTDSTG"
     gene            complement(191..583)
                     /locus_tag="PK345_06880"
     CDS             complement(191..583)
                     /locus_tag="PK345_06880"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HPX34774.1"
                     /translation="MKHANYFLNRLFAECCFLFKRIFLLFTRLTYNSISGDYDLDSEM
                     MIGLCPVSNTAQVQNVLHKILNYKPITLAWENNMLNVSGNNKSGKLSFINNLLRTTHC
                     FTGNGSLIFLILLNYEKHSICIIDFIRY"
     gene            complement(1142..1537)
                     /locus_tag="PK345_06885"
     CDS             complement(1142..1537)
                     /locus_tag="PK345_06885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34775.1"
                     /translation="MKYLKKIFRSPESRIPKSVKNGFLKHFPNATNIVWCKYPNLYEA
                     MFYDEEIEKITRFDKSGKLLECRVNSSFSEIPSFVKANIDPGYEIMSSIVVYASDMMN
                     YEFIVRDSELIRYSLIMDSLGNEIKLERL"
     gene            complement(1589..2167)
                     /locus_tag="PK345_06890"
     CDS             complement(1589..2167)
                     /locus_tag="PK345_06890"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HPX34776.1"
                     /translation="MIGKTKDYESLSDEDLVLKITQKGLTDLYSILYKRYYLKVLRKC
                     DSLVNDKDLSKVLAHDILSGVFEKLNSFQGKSSFNTWVYSITYNHCIDYLRSNKRLHY
                     PEWNRLQQLPEIVDVTEEDLTDLHYSRILELLDQLHTEEKAMLLMKYKDNLSIKDIAN
                     SMRLTESAVKMRLMRARVRLVFNYKSLYGSYP"
     gene            2353..2949
                     /locus_tag="PK345_06895"
     CDS             2353..2949
                     /locus_tag="PK345_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34777.1"
                     /translation="MKKQILFVFAALIMVALATSCQKVPQEQIDAAKAAIESVRAAQG
                     DLYVPEEFGALQDSLASAMAAIEVQKSKTFKNFDKVRTSLETIIQTAPTVIENVETAK
                     EQVKLATEEEIESIKGLLEENNLLMAKAPKGKEGKAVLLEIKNEMDMIENSIAEITEL
                     IATGDYLKAQAQAKAAKESLMGIHNELSEAIAKVGGKK"
     gene            3100..4224
                     /locus_tag="PK345_06900"
     CDS             3100..4224
                     /locus_tag="PK345_06900"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HPX34778.1"
                     /translation="MKFYMTKKARITTIILVIIFVLLGAGTIIAINMKPEAPIAEVEM
                     ARQALALARNQKAETFAKETFVKAQQLYDSAMIVWNNENEKFFLKRDYQQVRELATLS
                     KQQSDLAIEQAGKSFKNLETAIQRKLIYLNKLVSEIKVLSRFPLPLKITNSLSKGKLL
                     LNEAQATSRDGMLHQANNKLNEAENLLEHAHSKANSLIEDYFKNFDNWKMWKANTIRD
                     SKLNKSVAIVVDKYAGKCFLYKNGALTHEFDAELGKNWLGNKRQKGDKVTPEGQYKIT
                     DKKENSRTKYYKALLINYPNDDDKKRFQNEVKNGSLAANSKIGNLIEIHGHGGKGSDW
                     TDGCVALTNSDMDILFKVVQKGTPVTIIGSSITLAQIKKQ"
     gene            4285..4989
                     /locus_tag="PK345_06905"
     CDS             4285..4989
                     /locus_tag="PK345_06905"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HPX34779.1"
                     /translation="MLKVIKNKTLKKVLKISIGFLAGIIFILFVFWFFIIAVPASHDF
                     SIRQHKKYITLLPEETTKLYQGYITKTEKELQKLNTSYNSLTPNKPYLVINTTKNHFF
                     LYKSKQDLLREGFCSSGSYVQLISNDDRQWVFKTPKGMHTIKSKTRAPVWRKPDWAFI
                     EEGLPVPSATHSSRYEAGVLGDYALSLGDGYLIHGTIYKRFLGMPVTHGCIRLADDDL
                     EKVYNTLSIGSKVYIY"
     gene            4992..5822
                     /locus_tag="PK345_06910"
     CDS             4992..5822
                     /locus_tag="PK345_06910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34780.1"
                     /translation="MEKPEPKPTTNGDNKKNRIFKWILIAGFSIFFIFILYYFIMISL
                     AARKWISDFNKNYKTALLQSDSSSLQFITDSTFRTLHQQIAFTKARLDLTKANPLGLS
                     IDLSDSSAKLIIHGVTVHSSKIRQVKIARSLLAVEPYYLSLELSKPLKVKTEVSSIPK
                     EPIQIITAPKDTIEAAQISAIPDTTTTCSTFYKIIFENNLTLFVFQKPKTNKLFDLQW
                     ISFLYKARAPQVKANIVSILKFKIPEYSPEILIGLPEWEAKTIYRALPKEGLVALRLY
                     "
     gene            complement(6119..6904)
                     /locus_tag="PK345_06915"
     CDS             complement(6119..6904)
                     /locus_tag="PK345_06915"
                     /inference="COORDINATES: protein motif:HMM:NF024957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin family protein"
                     /protein_id="HPX34781.1"
                     /translation="MNRIGLKLVLVVFIYFLTPLSLFPQINRVPNLKLYDDEPLHFGF
                     ILAVNQMDFILKPKENIHQLYFKGTQIPEVDMKLTAIDSATVYGIEALPRLGFSVGIV
                     GDKRIGEYFNLRLIPSLSFGSRELLYDTRVFTKSGDTLQRVLNQKINSTFVEFPFYLK
                     YKSKRVHNFRAYVNTGIKYSFDLASQANKTEPNNYEPKLFRSDSYYMFSVGTDIYMNW
                     FKFGVELSMSYGMRDMLLRENNLYTNSIESLRSKIFMLTFTFE"
     gene            complement(6969..7985)
                     /locus_tag="PK345_06920"
     CDS             complement(6969..7985)
                     /locus_tag="PK345_06920"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-heptose synthase"
                     /protein_id="HPX34782.1"
                     /translation="MRYSTEDVKGIFEKFGQLSVMVIGDAMVDSYLWGKVSRISPEAP
                     VPVVEVTKRVNRLGGAANVALNLKALGAETYLCSVVGKDDKSKVIQDLLHENSLTSIG
                     IFQSGKRITTTKFRVIGSNTQMLRVDEEIDTMIDKAEQIVFFNLIKSIIEAKTINAII
                     FQDYDKGVITPDLIKKVVDLAQEKVIPIVVDPKRRNFLNYHHVDLFKPNLKELFDGMG
                     TPFVDFSLEKIRKAVRALHKKLHIPIVMTTLSDEGIFISRHLLDGSFSDHHIPAHLRS
                     IADVSGAGDTVVSVASLALAAGFSDYEIAAISNLAGGLVCESVGVVPVDKEKLMSEII
                     SKGL"
     gene            complement(8007..9215)
                     /locus_tag="PK345_06925"
     CDS             complement(8007..9215)
                     /locus_tag="PK345_06925"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HPX34783.1"
                     /translation="MIHHHLSNRVNSLSESATLQMTRRSRELKDAGIDVINLSIGEPD
                     FDTPESVKAAGIDAIQKNITHYPPVAGFNELRKAITAKLKRDNNLDYAMNQIIVSGGA
                     KQSIANALLCLVNPGDEVIIPAPYWVSYPEMVKLAEGKSVIIPSSIETNFKVTAEQVE
                     KYITPKSKLFIFSSPCNPSGSVYSKDELEAIAKVLAKKDDLYVVSDEIYEMINFVGKH
                     ESIAQFDFIKDRVVVVNGVSKGFAMTGWRIGYSASNKAIADACNTLQGQYTSGSATIS
                     QMASLKALQAAPSELVEVAEMQRQFRERRDYLLKLLADVPGMKTNVPDGAFYVFPEVS
                     YYIGKSDGDKIIKDDTDLCLYLLDKAHVALVPGSAFGGVNYIRFSYACSKDDLTESVK
                     RIKKALEQLK"
BASE COUNT         3135 a         1837 c         1599 g         2730 t
ORIGIN      
        1 tcccgtgcta tcagtattta ttatccatat atcacctttg ccatggtaat tggttgctcc
       61 agggatagaa tctaacaaat gtatggcaat tagtaatcca tcacctttgg aaacaatccc
      121 ataactcccg tctgactccg gtgaaccata acacttttgc caaactatct caggtatctg
      181 tgtgtatgca ttaatagcga ataaaatcaa taatgcagat agaatgtttt tcataattaa
      241 gtaagattaa gaaaataagc gaaccattcc ctgtaaaaca gtgagtggtt cgtaaaaggt
      301 tattaataaa tgacaattta ccggacttgt tattgcccga tacattcagc atattgtttt
      361 cccatgccag ggtaataggc ttgtagttaa gtattttatg taaaacattt tgcacctgag
      421 cggtatttga cacagggcag agtccaatca tcatttcgga atcaagatcg taatccccgg
      481 agatgctgtt gtaggtaagc cgggtaaaaa gaagaaatat tcgcttaaag aggaagcaac
      541 attctgcaaa aagccggttt aaaaagtaat tagcgtgttt cataagcgcc tccgcttaga
      601 tttttgtatt aaaatattga tgccaaatat agtaaaatgt tttaaaacga atgatatttt
      661 tcgtaaaaaa acatccaaat atcttaggca agttcaaaaa aaaagtcgga tttgcgcttg
      721 gtgattattg ttttaaaagc aaaaaggttg tttgtaacca atttcagcga tagtaacgct
      781 acccccgtta cctttaccaa ttgaggaaat cctatcgctt aacctgatta agatagtttt
      841 tagtcatagc atggctttaa gccatactat gacttttcta tgaattttcc ggcttactcc
      901 acatccgctt tggcttttaa acttacactg atgacttata ctccctcacc gaaagtgagt
      961 tgtaaagaat taactaatta atcaaattac gagataaaat tagaacttgg ttttatcgga
     1021 ttattttcaa ctattttgag ataggcaaaa aacaactctt ttcccaaaat acactaaaaa
     1081 cacacctctt atcaggggtt ataaaatttt tgaaaagcag atgataaatg attttgaggg
     1141 attacaatct ctccaactta atttcattcc ctaacgaatc catgataagc gaataacgta
     1201 tcaattcgga gtctctaacg atgaattcat agttcatcat atcagaagca tacacaacaa
     1261 tggaactcat tatttcgtac ccggggtcaa tattagcttt tacaaaagag gggatttccg
     1321 aaaaagagct attgacacga cattctaaca gtttcccaga tttatcgaaa cgggtaattt
     1381 tctcaatctc ttcgtcataa aacattgctt cgtacaagtt tggatacttg caccaaacaa
     1441 tattagtagc atttggaaaa tgttttaaaa agccattttt cacacttttc ggaatgcggc
     1501 tttcagggct tctaaatatt ttttttaaat atttcatctt aaaaaaactc agtattctga
     1561 cgatcaaatt ttaaaaagta acactatatc aaggataact tccatacaac gacttataat
     1621 taaacaccaa tctaactctt gcgcgcatga gcctcatctt aacagcgctc tcggtaagtc
     1681 tcatggaatt ggcaatatct ttaatcgaaa gattatcctt atatttcatt aacagcatag
     1741 ccttttcttc ggtatgaagc tggtcgagta attcaagaat acgcgaataa tgcagatcgg
     1801 taagatcctc ttcggttacg tcaactattt caggcaattg ctgtagtctg ttccactcag
     1861 gatagtgaag ccttttgtta ctcctcaaat agtctatgca atgattataa gtaattgaat
     1921 aaacccaggt attaaaagag gatttccctt gaaaactatt taatttttca aaaacacctg
     1981 aaagaatatc atgtgctaac acctttgata ggtctttatc attaacaagg ctatcacatt
     2041 ttcttaaaac cttcagatag tatcgcttat aaagaataga ataaagatcc gtcaggcctt
     2101 tctgtgtgat tttaagcacc agatcctcat cggataagct ttcataatct ttagtttttc
     2161 caatcatttt aaagccaaag gtttttaaaa cgacttcaaa aataaaattt ctttgttact
     2221 tttttcaaac agaccgtcta attaataata aaatttaata atgttgacga taaataatct
     2281 aaactttgta attttgcgaa caattgaatt ttaacaaatt attaattatt aactttttat
     2341 tatttaaaaa ctatgaaaaa acaaattttg ttcgtttttg cagcgcttat aatggttgct
     2401 cttgctacaa gttgtcaaaa agttccccaa gaacagattg atgctgccaa agcagctatt
     2461 gaatccgtaa gggctgctca aggcgacctc tatgttcctg aagaatttgg cgcattgcag
     2521 gattcgcttg catcagctat ggccgctatt gaagtgcaga aatcaaaaac atttaagaat
     2581 ttcgataaag tgcgtacttc tcttgagacg atcattcaga ccgcacccac agtaattgaa
     2641 aatgtagaaa ctgcaaaaga acaggtaaaa ttagctacag aagaagaaat tgagagtata
     2701 aaagggctat tggaagaaaa caacttactg atggctaaag ctccaaaagg taaagagggc
     2761 aaagcagttc tgcttgagat caaaaacgaa atggatatga tcgaaaattc tatagcagaa
     2821 attactgaat tgattgccac aggcgattac ctaaaagcac aagcacaagc aaaagcagca
     2881 aaagagagcc ttatgggcat tcataatgag ctttctgaag ctatagcaaa agttggtggt
     2941 aagaaataat cactgttaat gtaaattttt tctttaaaag aacctcgaca atattgtcgg
     3001 ggttctttta gttattctct ttagtttttt tcaaaaagat gtatccctct aaaaattgaa
     3061 taaaagtaag aaacattaat taccactaaa attctttttg tgaaatttta tatgactaag
     3121 aaggcgcgga taacgacaat cattttagtg attatttttg ttttgttagg tgcgggaact
     3181 atcattgcaa taaatatgaa gcccgaagcc ccgatagctg aagttgaaat ggcgcgacag
     3241 gctctggcat tagcccgaaa tcaaaaagcg gaaacctttg ctaaagagac atttgtcaaa
     3301 gcccaacaac tttacgactc ggcaatgata gtatggaata acgaaaatga aaaattcttt
     3361 ttaaaaagag actaccagca agtacgcgaa ttagctacac tctcaaaaca acaatctgat
     3421 ttagcaattg aacaggctgg caagtcattt aaaaacctcg agacagctat ccaaaggaag
     3481 ctaatatacc tcaacaaatt ggtatctgaa ataaaagtat tatcccgttt tccgctccca
     3541 ttaaaaatta ccaacagtct ctccaaaggg aagttgttac taaatgaagc gcaagctacc
     3601 agtagagatg gaatgcttca tcaggcaaac aataaactaa acgaagctga aaatctatta
     3661 gaacacgctc actcgaaagc caacagtctt atcgaagatt attttaaaaa ctttgataac
     3721 tggaaaatgt ggaaggctaa caccatcaga gacagcaaac ttaacaaaag cgttgcaata
     3781 gtggttgaca aatacgccgg caaatgcttc ttatataaaa acggagcttt gacccatgag
     3841 tttgatgccg aacttggaaa aaactggctt ggcaacaaaa ggcaaaaggg tgataaggta
     3901 acgccggaag gacagtataa aataacagat aaaaaagaaa attcccgtac taagtattat
     3961 aaagcgctgc tgattaatta tcctaatgat gatgataaaa agagatttca aaatgaggta
     4021 aaaaatggat cacttgcagc aaattcgaaa atcggaaatt taatcgaaat tcatgggcat
     4081 ggcggcaaag gtagcgactg gactgatggg tgcgtggctt tgacaaactc cgatatggat
     4141 atattattca aagtcgttca aaaaggaaca ccagtaacca ttataggctc ctcaattact
     4201 ctggcgcaga taaaaaaaca ataactaaac agagtctatt aaaggataat aaaaacagaa
     4261 tccgaactaa tttttaacta acaaatgctg aaagtcatta aaaataaaac gcttaaaaaa
     4321 gtattaaaaa ttagtattgg ttttttagcc ggcattattt ttatcttgtt tgttttttgg
     4381 ttttttatca ttgccgtacc ggcatcccac gatttttcca ttcgtcaaca taaaaagtat
     4441 attacacttt tacctgagga aaccacaaaa ctttatcagg gctatatcac caaaaccgaa
     4501 aaagagcttc aaaagttaaa tacatcctat aattcattaa ctcctaacaa accatattta
     4561 gtaattaaca cgactaaaaa tcattttttt ctatataagt caaaacaaga ccttttaaga
     4621 gaaggatttt gcagttcggg aagttacgtt cagctcattt cgaatgatga tcgtcaatgg
     4681 gtttttaaaa ccccaaaagg gatgcataca ataaaaagca agactcgtgc tcctgtttgg
     4741 agaaaacccg attgggcatt catcgaagag ggtttgccag taccatcggc cacacactca
     4801 tcgcgttatg aggctggcgt tttaggcgac tatgcactca gcctcggaga tggatacctg
     4861 atacacggca ctatttataa aagattcctt ggaatgccag taactcatgg ctgcatccga
     4921 ctggccgatg atgacctgga aaaagtatat aatacactta gcataggatc aaaagtatat
     4981 atctattaaa aatggaaaag ccagaaccga aacctaccac aaacggtgat aacaaaaaaa
     5041 accgaatatt taaatggatt ttaatagcag gcttctctat cttttttatt ttcatcctgt
     5101 attactttat catgatatcg ctggcagcga gaaaatggat ttcggatttc aataaaaatt
     5161 ataaaacagc gctactacaa agtgattcaa gtagccttca atttattact gactcgacat
     5221 ttcgtacgtt gcaccaacaa attgccttta caaaagcacg tcttgatctg acaaaagcca
     5281 atcctttagg attaagtatt gatctttctg acagcagcgc taaattaata atccatggtg
     5341 ttaccgtgca ctcttcgaaa atacgacaag taaagatcgc tcgtagtctt ttagccgttg
     5401 aaccatatta tttatcgctg gaattatcga aaccattaaa agtaaaaacc gaagtttcat
     5461 ccatcccaaa agagcctatc cagataataa cagccccgaa agacacaatt gaggctgcac
     5521 aaatttccgc aatacctgat acaactacta cttgttcgac attttacaaa ataatatttg
     5581 aaaacaacct cacattattc gtatttcaga agccgaaaac taacaaattg tttgacttgc
     5641 agtggatttc gttcttatat aaagcaagag ccccacaggt taaagccaac atagtgagta
     5701 tcctcaaatt taaaataccc gaatattcac cagaaattct tatcggactt cccgaatggg
     5761 aagccaaaac aatttatcgg gcattgccca aagagggttt ggttgctttg cgactttact
     5821 gattttgaac tttatccgca gctcggtaag aaactcgaaa ccttttcccc ggcttaaatt
     5881 tcatattatt gagcctgcct ggatttcgta aacattcaat ataaagcata ctcatcaaat
     5941 catgatttcc ggagaactcg aaaacgttag tatgcattat aaaaattaga ctatcgcaac
     6001 caaattttag ttctgcttaa aattcaccac gatttttata ctaaaacggg atataacgtt
     6061 agccataaag ctaaaattcc tgagaatcta tcatcagttt tttttgatgt tgagaaaact
     6121 attcgaaggt aaaagtaagc atgaaaatct ttgatcgcag actttcgata ctattggtgt
     6181 agaggttatt ttccctgagc agcatatccc tcatgccata cgacatggaa agctcgacac
     6241 cgaatttaaa ccagttcatg taaatgtcag tgccaacgct aaacatataa taactgtcgg
     6301 atcgaaaaag ttttggttcg tagttgttcg gttcggtctt gtttgcctgc gaagctaaat
     6361 cgaatgagta ttttattcca gtattaacat atgctctaaa attgtgtacc cttttcgatt
     6421 tatatttcag gtaaaaaggg aattccacaa atgtagaatt gattttttga ttaagtacgc
     6481 gctgtagggt atctccgctt tttgtaaaaa cacgtgtatc ataaagtagc tctctgctgc
     6541 caaatgacaa gctggggata agccgcaaat taaaatattc ccctatccgc ttatccccta
     6601 ctattcccac cgaaaagcct aaacgtggca aagcctcaat accataaacc gttgcagaat
     6661 caatcgctgt tagtttcata tcaacctcag ggatctgagt ccctttgaag taaagctgat
     6721 ggatgttctc ttttggtttc agtataaaat ccatttggtt cactgccaga ataaaaccaa
     6781 aatgcaacgg ttcatcgtcg tagagtttaa gattgggaac tctgtttatc tgcggaaata
     6841 acgataaagg agtgagaaaa tatataaaaa ctactaatac gagctttaag cctatcctgt
     6901 tcaaatgctg atttttatta atttcttgaa ataacgcctc tattatgctt ttattttcaa
     6961 gctattgatc agagtccttt acttattatt tctgacatca gtttttcttt atccaccgga
     7021 accacaccta cgctttcgca gactaatccg cctgccagat tactaatggc ggcaatctca
     7081 taatcgctaa acccggcagc aagcgcaagg gatgcaacac taacaacggt atcgccagcg
     7141 ccggaaacat cagctatcga acgcagatga gccggtatgt gatgatcaga aaaactacca
     7201 tcaagcagat gccggctaat aaaaatacct tcgtccgaaa gggtagtcat cacaattgga
     7261 atatgtaact tcttatgcaa agctcgtacg gcttttctaa tcttctcaag gctgaagtca
     7321 acaaaagggg tacccatgcc atcaaacaat tctttaagat ttggcttaaa aagatctaca
     7381 tgatggtaat taagaaaatt tcttctctta ggatccacca ctatgggaat cactttttcc
     7441 tgagccagat ccacaacctt ttttataagg tcgggggtaa taacgccctt gtcataatcc
     7501 tgaaaaatga ttgcatttat cgttttagct tcaataatac tttttatcaa attaaaaaat
     7561 acaatttgct cggccttatc aatcattgta tctatctctt catccacgcg aagcatttgg
     7621 gtattactac cgattacccg aaatttcgta gttgtaatgc gctttcccga ctgaaaaatt
     7681 ccaatagacg taaggctgtt ttcgtgcagc aagtcctgaa ttactttgga tttatcatcc
     7741 ttgccaacta ccgaacacag ataagtttct gctcctaagg cttttaggtt gagcgcaaca
     7801 ttagctgctc cgccaagacg attaacacgc tttgttactt ctacaactgg aacaggcgcc
     7861 tcaggcgaaa tacggctgac ctttccccaa aggtatgaat caaccattgc atcgccaata
     7921 accataaccg acaattggcc aaacttctca aaaatacctt ttacatcttc tgtcgaataa
     7981 cgcatcacgt aactatttgc tattgcttat tttaattgtt caagcgcctt tttgatcctt
     8041 ttcaccgatt cagtaaggtc atcttttgaa caggcataag agaacctgat ataatttaca
     8101 ccgccaaatg ccgaaccagg caccaaagca acatgagcct tgtcaagaag gtaaaggcat
     8161 aagtcagtat catcctttat tattttatct ccatccgact tcccaatata gtacgacacc
     8221 tcaggaaaca catagaatgc tccatccgga acgttggtct tcataccggg aacatcagct
     8281 aaaagtttta aaagataatc gcgacgttcg cgaaactgtc tctgcatctc agcaacttca
     8341 accaattccg aaggcgccgc ctgaagcgct ttcaatgatg ccatttgcga aatagtggca
     8401 gaaccacttg tgtattgccc ttgtaatgtg ttacatgcat cagcaatggc tttgttggaa
     8461 gcagaatagc cgatacgcca gccagtcatg gcaaagccct tcgaaacacc attgacaaca
     8521 acaacacgat ccttaataaa atcaaactga gcaatgcttt catgcttgcc cacaaaattg
     8581 atcatctcat aaatctcatc agaaactaca tagagatcat ctttttttgc cagaacttta
     8641 gcaatagcct ccaactcatc cttactgtaa accgatccgg aagggttaca tggtgagctg
     8701 aagatgaaca gcttcgattt cggtgtgata tacttctcaa cctgttcggc tgtaacttta
     8761 aagtttgtct ctattgatga tgggataata actgacttcc cctcagccag cttcaccatc
     8821 tcaggatacg acacccaata cggcgcaggt attattactt catcgcccgg gtttacaagg
     8881 caaagtaatg cattagcaat agactgcttg gctccacccg aaacgataat ctgattcatg
     8941 gcataatcga ggttattatc acgcttcagc ttagcggtaa tggccttacg taactcattg
     9001 aaaccagcca ctggaggata atgagtaata tttttctgaa tagcgtcaat accagccgcc
     9061 ttaacactct cgggagtatc aaaatcaggc tctccaatac ttaggttaat tacgtcaata
     9121 ccggcatctt ttaactctct gctccgacgg gtcatctgca aagtagccga ttctgataaa
     9181 ctattcacac ggttagaaag atggtgatga atcatgtctt aaatatttta atgattaaaa
     9241 ttaagatcgc aaagctaaat aaaatgaaaa tgtaaatagc aataagacca agattttgat
     9301 a
//
LOCUS       DAOOAI010000088         9145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_9365_length_9145_cov_4.899780, whole genome shotgun sequence.
ACCESSION   DAOOAI010000088 DAOOAI010000000
VERSION     DAOOAI010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 9145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9145
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9365_length_9145_cov_4.899780"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1751
                     /locus_tag="PK345_06930"
     CDS             39..1751
                     /locus_tag="PK345_06930"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPX34784.1"
                     /translation="MKSLLVTILALFSFTCLYSQIYPDKYFVAFTDKNNSPYSIENPQ
                     EYLSARAIERRSRYNIPIDIHDLPVNPQYIEAVADIGVQVINPTKWLNGITIYTNDSS
                     LLSEIEALPFVKSVRKARQATQAHPKTDFDDDISLFKPSWENETIIDFSNRTKATGKS
                     TSLQYGLGYNQINQINGIPLHDDGFQGQGMVIAVLDAGFSYANQLNAFDSLYMNGLIY
                     GTRDIVSGGTNVYQGSFHGTGVLSTMGANLPGELIGTAPKAGYYLIRTEDTGSEYLIE
                     EYNWASGAEFADSTGADIINSSLGYVDFDDPNQNHIYQDMDGNTAPATIAADRAMSRG
                     MIVVNSAGNSGNYTSWPWIGAPADGDSVFTIGAVDANGIIAGFSSIGPTADGRLKPNV
                     VAQGAATVIASSNGGTTTSNGTSFSSPIIAGMTACLWQINQELANMSILEAIQQSGSY
                     YNNPNYQYGNGIPDYNQARMVLSAQNKGIALTPESIKVIPNPFFDKIGILFNAIDSQL
                     VTIRMFDITGRLIVSGKFDVQRVGLNYLELTQLDFIKPGIYTLTVIAGEKRLNSQKIV
                     KVKD"
     gene            complement(1755..2093)
                     /locus_tag="PK345_06935"
     CDS             complement(1755..2093)
                     /locus_tag="PK345_06935"
                     /inference="COORDINATES: protein motif:HMM:NF013361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpyruvate tautomerase MIF-related protein"
                     /protein_id="HPX34785.1"
                     /translation="MPLLKIISNTDIADKNAFIGQSSKEIARILGKTEKYVMVIFTFN
                     PNFIFSTSDEPALYVELKSIGLPTEKTGEISRKIMDFLSSKTGVIHSRIFIEFTDVPP
                     NLWGWNGGTM"
     gene            complement(2112..2516)
                     /locus_tag="PK345_06940"
     CDS             complement(2112..2516)
                     /locus_tag="PK345_06940"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX34786.1"
                     /translation="MRYFFTLFAIVFLLTSCNNKEDRSSKLIRDGIQKLYAAEFEEAM
                     EIFQKSAKLNPENPEAWFYIGAMYQNKQDFNTAIEYYSKAIDLKNDYADAYYNRGQCW
                     FYLGDRNNSCDDWLLAENYGRENLNELLDKCN"
     gene            complement(2547..3590)
                     /gene="mltG"
                     /locus_tag="PK345_06945"
     CDS             complement(2547..3590)
                     /gene="mltG"
                     /locus_tag="PK345_06945"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HPX34787.1"
                     /translation="MRTRTKIFIGVLAFVFLIIIGAGFFLYKIVLSDNIKLPDNKPGY
                     LFIPTGATYADMLDSIESTGYIKNIQFFRWMALHKKLDAHVHPGRYEILDGMSNNDLI
                     NMLKSGRQKPLNVTFNNITFPPQLAGSISKQIEADSIAIIRLFNNEKYLASYGLTKET
                     APIIFIPNTYEFYWNTTADGFFKRMKEEYDKFWTNERKLRAELLNLSPLEITIIASIV
                     EKETNKNDEKERIAGVYLNRLKRGWKLQADPTTVYAVYRETGNILNRVLWVHTQLDNP
                     YNTYVYSGLPPGPICFPSISSIDATLKPEKHQYMFFVASSDFSGYHRFSTNYNQHQRY
                     VREYQLALRQQLQ"
     gene            complement(3599..4117)
                     /locus_tag="PK345_06950"
     CDS             complement(3599..4117)
                     /locus_tag="PK345_06950"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HPX34788.1"
                     /translation="MIKGKEILLRAPEMSDVDMLYNLENDLSIWRLSNTNAPLSRFAI
                     EQYILNAGLEIFETRQVRFMIVNLSDEKAIGTIDLFDYEPIHRRAGIGIYLEANERGK
                     GKASEALDLVIDYCFNTLMLRQVYCNISPDNVQSKILFEKKGFTLIGTKRSWLLIQNQ
                     WMDEDMYQLINL"
     gene            complement(4148..4933)
                     /gene="dapF"
                     /locus_tag="PK345_06955"
     CDS             complement(4148..4933)
                     /gene="dapF"
                     /locus_tag="PK345_06955"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008837 - diaminopimelate epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HPX34789.1"
                     /translation="MVEFYKYHGAGNDFILIDNRSKVFNPDKKIIKNLCRRHLGVGAD
                     GLILLEDDSLSGYMMRYFNSDGKEASMCGNGGRCFVAFAQSLGLCNDHFIFNAIDGYH
                     EAIILKRDKDSYLVNLKMNDVSTVLPYDEGFYLDTGSPHLVVFTNYINKIDVFKTGKE
                     LRLLPKWGADGVNVNFAQIENNKIISRTYERGVENETLACGTGAVAIAISAVDQNLIR
                     NSPVKIEMCGGTLKVHYEKREQKFTNIWLEGPAVMVFKGGINL"
     gene            5173..6624
                     /locus_tag="PK345_06960"
     CDS             5173..6624
                     /locus_tag="PK345_06960"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009319325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HPX34790.1"
                     /translation="MKKFASLVLVALLGGLISVGVYRVFDKKTDESSVYPIVQQAPSQ
                     VYNTSYLPTYAVPENVPDFVNAADLTVHAVVHIKTEVSRRNSIYDEFFYEFFGMPLKP
                     SQPLVATGSGVIIANDGYIVTNNHVVQNAVRLEVTLNDKRSFDAKIIGADPTTDLALI
                     KIEATGLSYLSYGDSDALRVGEWVLAVGNPFNLTSTVTAGIVSAKAREINILGAAGAI
                     ESFIQTDAPINPGNSGGALVNTRGELVGINAAIASNTGSYAGYSFAIPVNIVKKVVND
                     FINFGEIQRAYIGVSIRDIDSHFAQELGLDAPQGVYVVSVVEHSSADDANIKAEDIIL
                     EIDNKPINSVSRLLETIGQRNPGDKIQVKILRNKKVINKELLLKNRDNTTDMVAKKDS
                     KEIDILGATFERVKDDELSRLGIDNGVMVKKIRRGELMNKGVREGFIITHIDKAPVVT
                     TDDIIRLLNGKRGGVLLEGIYPNGKRSYYAIGL"
     gene            6840..7706
                     /locus_tag="PK345_06965"
     CDS             6840..7706
                     /locus_tag="PK345_06965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006949282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD/SigA"
                     /protein_id="HPX34791.1"
                     /translation="MRQLKITKSITNRETASLDKYLQDIGREELITAEEEVLLAQRIK
                     QGDQSALEKLTKANLRFVVSVAKQYQNQGLSLPDLINEGNLGLIKAAQRFDETRGFKF
                     ISYAVWWIRQSILQALAEQSRIVRLPLNQVGSLNRIRKESSKLEQKFERPPSTDEIAE
                     SLDLPETKVESVLKITTRTVSMDAPLTQDEDMSLLDVYVSDDNPTTDRELMKESLARE
                     IQRSLATLTKKERDVINLYYGIGMNHGLTLDEIAMKFDLTRERVRQIKEKAIRRLKHT
                     SRSKLLKAYLGQ"
     gene            7946..8788
                     /locus_tag="PK345_06970"
     CDS             7946..8788
                     /locus_tag="PK345_06970"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HPX34792.1"
                     /translation="MKNLKTVLKAIFPALIIFFFGQQVSGQIFGTVGSKNVITQERQL
                     PDFNSIRIKGTTDVVIRQGDKTAVVVKADDNLIKNIITKVQGDFLVVTAEGSIRSYKK
                     FEVLVTINRLEAIEVYGSGDVESEGIISGHELSILVNGSGDVELNLDFKKLTANINGS
                     GDIEVSGINGDLNANINGSGDFEASSVRLNDCKISVKGAGNVKMQGSAAIVMLNLAAS
                     GRIELQRLISEDVNVQSSGSGNVYVNVNGSLNARLSGSGNLKYLGNPVSVDVLSTGSG
                     SVYK"
     gene            complement(8879..>9145)
                     /locus_tag="PK345_06975"
     CDS             complement(8879..>9145)
                     /locus_tag="PK345_06975"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HPX34793.1"
                     /translation="KDANLFFTSYKEGWKTDRGMIYMIFGAPTEVFKKSEIETWIYGD
                     PQKRTRLHFDFIQNKNPFTTNDFELVRQPDYKNPYFVAVDYLRR"
BASE COUNT         2944 a         1673 c         1765 g         2763 t
ORIGIN      
        1 aaaacaaata ctcattatta aataaaacac tatcaagcat gaagtcctta ttagttacaa
       61 ttttagcttt attctcattc acttgtctat attctcaaat ttatccagac aaatattttg
      121 tcgcttttac tgataaaaac aactcgcctt attctattga gaatccacag gaatatcttt
      181 cagcaagagc gattgagcgg agatcaagat ataatatacc cattgatatt catgatctac
      241 cggtaaatcc acaatatatt gaagctgttg ccgacattgg cgtacaggtt atcaatccga
      301 ccaaatggtt gaatgggatt acaatttata ctaacgattc ttctttactg tcggaaattg
      361 aagcgctccc atttgtgaag tccgtaagaa aagcgcgaca agctacacaa gctcatccaa
      421 aaactgattt tgatgatgac atttctttat ttaagccttc atgggaaaat gagactatta
      481 ttgatttttc taataggaca aaagctactg gaaagagtac ttcacttcaa tatggccttg
      541 gttacaacca aattaatcag atcaatggta ttcctctcca cgacgacggt tttcaagggc
      601 aagggatggt cattgcagtg ttagatgcag gtttttctta tgccaatcag ttgaacgcct
      661 ttgattcact ttatatgaac ggccttattt atggtacaag ggatattgtt agcggtggaa
      721 caaacgttta tcaaggcagt tttcatggca ccggagttct ttctacaatg ggtgcaaacc
      781 tcccgggaga gcttatcgga acggctccca aagccggata ttaccttatt cgtactgaag
      841 ataccggatc ggaatacctt atcgaagagt acaactgggc ttctggagct gaatttgctg
      901 atagtaccgg tgctgatatt ataaactcat ccctcggata tgttgatttt gacgatccta
      961 accaaaacca catttatcag gatatggatg gtaatactgc ccctgcaact attgctgccg
     1021 atcgtgctat gagccgcgga atgatagtag taaacagcgc tggaaattct ggcaattaca
     1081 cttcatggcc atggattggc gctcctgctg acggcgacag cgtctttacc ataggagcag
     1141 tagatgcaaa cggtatcata gcagggttta gctctattgg ccctactgca gacggaagac
     1201 taaagccaaa cgtagtagca caaggagccg caacagtgat cgcctcctca aatggcggaa
     1261 cgacaacctc aaacgggaca tcattttcat ctccgataat tgcaggaatg accgcgtgcc
     1321 tgtggcagat aaaccaagaa ttggctaata tgagtattct tgaggcaatt cagcaatcag
     1381 gaagctatta taataaccca aattatcaat acggaaacgg aataccagat tataatcaag
     1441 caagaatggt gctttcagct caaaataaag gcattgctct aactccagaa agtataaagg
     1501 ttatacctaa tccgtttttc gataaaatcg gaatactctt taacgcaata gattcccagt
     1561 tggttacgat caggatgttc gatattacag ggcggttgat agtatccgga aaatttgacg
     1621 ttcaacgtgt ggggctaaat tatctcgaac taacacaatt ggattttatc aaaccgggta
     1681 tttatacatt aactgttata gcaggagaga agaggctaaa ttcacaaaaa atagtaaagg
     1741 taaaagatta gttgttacat agtaccgccg ttccaacccc acaaatttgg tggcacatcg
     1801 gtaaattcga taaaaatcct cgaatggatg acacctgttt tggaagaaag gaaatccata
     1861 attttacgtg aaatctcccc agtcttctct gttggtagtc ctatactttt cagttcgaca
     1921 taaagcgctg gctcatccga tgttgaaaaa atgaagtttg gattaaaggt aaaaattacc
     1981 attacatatt tttctgtttt tcctaaaatc cgggcgatct cttttgacga ttgacctatg
     2041 aaagcatttt tgtcagcaat atcagtgttt gaaatgattt ttaacaatgg catatcctct
     2101 attttaaatt gttaattaca tttatcgagt agctcgttaa gattttcgcg accatagttt
     2161 tcagccaata gccagtcgtc gcaagagttg ttacgatcgc ctaaataaaa ccaacactgc
     2221 ccccgattat aatatgcatc agcataatca ttcttaagat caatggcttt cgaataatat
     2281 tctatagccg tattaaagtc ttgtttattt tgatacatag ctccgatgta aaaccaagct
     2341 tcaggatttt ctggattcaa ctttgctgat ttctgaaata tctccatggc ctcttcaaat
     2401 tcggctgcat aaagtttctg aatgccatct ctgatcagct tacttgacct atcctccttg
     2461 ttgttgcatg aagtgagaag aaatacaata gcgaaaagtg taaaaaaata acgcattact
     2521 ttgaggtatt atatataatt cattacttat tgaagctgtt gtcttagcgc aagttgatat
     2581 tcacgaacgt aacgttgatg ttgattataa tttgttgaaa aacggtgata cccagaaaaa
     2641 tcagaactgg cgacaaagaa catatattga tgcttttcgg gtttaagcgt ggcatcaata
     2701 ctgctgattg acgggaagca gatcgggcct ggcggtaatc cactgtaaac ataagtattg
     2761 taaggattat ccaattgtgt gtgcacccaa agtacacgat tcaagatatt accggtttcg
     2821 cgataaacag cgtaaactgt agtaggatcg gcttgtagct tccaaccacg tttaagacga
     2881 tttagataaa cccctgcaat acgttccttt tcatcatttt tatttgtttc tttctccaca
     2941 attgaagcaa taattgttat ttccaatgga gatagattaa gcaactcagc cctcagtttt
     3001 ctttcgttag tccagaattt atcatattct tctttcatac gtttgaaaaa accatcggcg
     3061 gtagtgttcc agtaaaactc ataagtgtta ggaataaaga tgataggcgc agtctctttt
     3121 gtcaatccat aactggctaa atacttttcg ttattaaata atctgattat cgcaatcgaa
     3181 tctgcttcta tctgcttgga tattgagccg gcaagttgag gaggaaaggt gatattgtta
     3241 aaagtaacat ttaagggttt ttgcctgccc gatttaagca tgttgatcag atcgttgtta
     3301 ctcataccat caagaatttc gtacctaccc ggatgaacat gagcatcaag ctttttatgt
     3361 agcgccatcc atctgaaaaa ctgaatattt ttaatgtacc cggttgattc aatggaatca
     3421 agcatatcgg cgtaagtggc tccagtagga ataaacagat aacccggctt gttgtcggga
     3481 agctttatgt tatcggaaag cactatttta tataaaaaaa agccagcgcc tattattatc
     3541 aagaaaacaa aggctaaaac accaatgaaa attttcgttc ttgttctcat gttaaatatc
     3601 aaagattaat taattgatac atgtcttcat ccatccattg attttgtatt aacaaccaac
     3661 ttctctttgt cccaataagt gtaaatcctt ttttttcaaa aagaattttg ctttgcacat
     3721 tatctggaga aatgttacaa tatacttgcc tgagcatcaa tgtattaaaa caataatcta
     3781 taaccagatc aagcgcctca gatgctttac ctttccctct ttcatttgct tcgagataga
     3841 tacctatccc tgcccgccga tgaattggct cataatcgaa aagatcaata gtgccaattg
     3901 ctttttcatc agaaaggttg actatcataa acctcacctg acgcgtttca aaaatttcta
     3961 aacctgcatt aaggatatac tgttcaatcg caaacctaga cagtggagca ttagtattgc
     4021 ttaaacgcca gatagaaaga tcgttttcca aattataaag catatcaaca tcggacattt
     4081 ccggcgctct gagtagtatt tctttgccct tgatcatggt aatcaaaata tggtagcttt
     4141 tgttgtttta taaattaata ccccctttaa ataccataac tgcaggccct tcaagccaaa
     4201 tatttgtaaa cttttgctct ctcttttcat aatgaacttt taatgtacca ccacacattt
     4261 caattttaac aggcgaattt cgaatgaggt tttgatccac agcagatatg gcaatagcaa
     4321 cagccccagt tccacatgct aacgtttcat tttcaacgcc cctttcataa gtgcggctaa
     4381 ttattttatt attttcaatt tgagcaaaat taacatttac accgtcagcg ccccattttg
     4441 gaagcaacct gagttctttt ccggttttaa aaacgtcaat tttgttaatg taattggtaa
     4501 aaacaactaa atgaggtgaa ccggtatcga gataaaatcc ttcgtcgtaa ggtaaaacag
     4561 tactcacatc attcattttt aggttaacta aataggaatc tttatctctt tttaaaataa
     4621 ttgcttcatg atatccatca atagcattaa aaataaaatg atcattacag aggcctaagg
     4681 attgtgcaaa agctacaaaa catcgtccgc cgttaccaca cattgaggcc tctttgccgt
     4741 cggaattaaa atatcgcatc atgtaacccg acaaactatc atcctcaagc agtataagcc
     4801 cgtcagcgcc aactccgagg tgtctccggc agagattttt aatgattttt ttgtcaggat
     4861 taaaaacctt agaccgatta tcaataagga tgaagtcgtt tccggcgcca tgatatttgt
     4921 aaaactcaac cactttcttg cttttatttt gatacaaaac taataattct tgcgtttaag
     4981 caagttgtaa ccaaaatatt ggattaataa cttttaactt tggtttatat aaattcgact
     5041 cactaaaaac gttaagaaga tatacatttt ttaaccaatt gaggaaggaa aaagaggatt
     5101 acctttatgt gttctggtgg gaattggctt gaaaattgat attaacggaa aaattaagaa
     5161 aagatcatta ttatgaaaaa gtttgcatca ttagttttgg ttgccttact tggcggatta
     5221 atttcagttg gtgtttacag agttttcgat aagaaaacag atgaatcttc tgtttatcca
     5281 atagttcagc aagctccttc gcaggtttat aatacgagtt atttaccaac gtatgcagta
     5341 cctgaaaatg tccccgattt tgttaatgct gccgatctga cagttcatgc tgtcgttcac
     5401 attaaaactg aagtgagtcg aaggaattct atctatgacg agtttttcta tgaatttttt
     5461 ggcatgcccc tcaagcccag tcaaccgctt gttgctaccg gttcgggtgt aataatcgcc
     5521 aatgacggct atatcgttac caacaatcac gttgtacaaa atgcagtaag attagaagtt
     5581 acacttaacg acaaaaggtc gtttgatgca aaaatcattg gagccgatcc taccaccgat
     5641 ctcgcactga ttaaaattga agcaaccggc ttgtcatatc tttcatacgg cgattcggat
     5701 gctttgcgag taggagagtg ggtgctggct gttggtaatc cttttaatct tacatccaca
     5761 gttacggcag gtattgtaag cgccaaagca cgagaaatta acatcttggg agctgccgga
     5821 gcaatagaat catttattca aacagacgct cctataaacc ccggtaacag tgggggtgcg
     5881 ttagtaaaca cacgtggcga attagtaggc atcaatgctg cgattgcttc aaacacaggc
     5941 agctatgccg gttactcatt cgctatacca gtaaacattg taaaaaaagt ggtaaatgat
     6001 tttatcaact ttggtgaaat acaaagagca tatattggtg tgtcaatccg tgatattgac
     6061 agccattttg cacaggaatt aggacttgat gcgcctcagg gcgtttatgt ggtttcggtc
     6121 gtggaacaca gttcagccga tgatgctaat ataaaagctg aagatatcat acttgaaatt
     6181 gataacaagc ctattaattc agtctcaaga ttacttgaaa ctattggtca gcgaaacccg
     6241 ggcgataaaa ttcaggtaaa aatattacga aacaaaaaag taattaataa agagcttctg
     6301 cttaaaaaca gagacaatac cactgatatg gttgcaaaaa aggattcaaa agagatagac
     6361 attttaggcg ccaccttcga aagggttaaa gatgatgaac taagcagact tgggattgac
     6421 aacggagtta tggttaaaaa aatcagaaga ggcgaactaa tgaacaaagg cgttagggaa
     6481 gggtttatta ttacacacat agataaagcg cctgtggtta ctacagatga cattattaga
     6541 ttacttaatg gcaaacgcgg tggcgtactg ctcgaaggta tctatcctaa tggcaagcga
     6601 tcttattatg ccataggtct ttaaaaaagt ttgaatagga aaatattctt cttaatttca
     6661 agaaattttt atccatccgg aaacataagg ccggatggtt tgttctattt taagaattaa
     6721 gattatttga atacaatatt ttgttgtcgc taatagttat ttcaagcgat aataattggt
     6781 tataaactaa ataagaaaac aatagaaaac aattttacta acaaaacaag gacggtttaa
     6841 tgagacagtt aaagattacg aagtctatta ctaatcgcga aacggcttcg cttgataagt
     6901 atcttcagga tataggacga gaagagctta tcacagccga agaggaggtt ttgcttgcgc
     6961 aacgtatcaa gcagggcgat cagtcagctc ttgagaagct gacaaaagct aacctgcgtt
     7021 ttgtcgtttc tgttgcaaag caatatcaaa atcaagggct tagccttccc gaccttatta
     7081 atgaagggaa tcttggcttg attaaagctg cccagcgatt tgatgaaaca cgaggcttta
     7141 aatttatctc ttatgctgtt tggtggatca gacaatctat ccttcaggcg cttgcagaac
     7201 agtcgaggat agtaagatta cctcttaatc aggtaggttc acttaatagg attagaaaag
     7261 aatcttcgaa gcttgagcaa aaatttgaaa gacctccttc aaccgatgag attgcagaat
     7321 ctctcgacct gccggaaacg aaagtggaga gcgtgcttaa aataaccact cgcactgtgt
     7381 caatggatgc tccgcttact caagacgaag acatgagcct attggatgtg tatgtatcag
     7441 atgacaatcc tacaaccgac agagagctga tgaaagaatc gctggcacgt gaaatacaac
     7501 gatcgttggc cacgctgacc aagaaagagc gagacgttat caatctctac tatggaatag
     7561 gaatgaatca tgggcttaca cttgacgaaa ttgccatgaa atttgatctg acgcgcgagc
     7621 gtgtacgaca aataaaagag aaagctattc gaaggctgaa acatacctcg agaagcaaac
     7681 tgctcaaagc ctatttaggg cagtaataat taattaaatt aatatatagt aaattgagac
     7741 ccggtaaaac gccgggtctt gtttttattc tcaatagaaa accttttttt aagctgagat
     7801 aattttggaa gagcttactg acctaaaaaa aactctaatt ttttttcaaa aacctgtaac
     7861 aaaataaaaa aaatgttgtc ctattagatt gaaattgatt tcaattaaaa aattatcact
     7921 ttgaaacaaa ttaaactaaa caaagatgaa aaacctaaaa acagttttaa aagcaatttt
     7981 tccggcattg ataatattct ttttcggaca gcaggtttct ggtcagattt tcggtacagt
     8041 tggtagcaaa aacgtaataa cacaggaaag gcaacttcct gattttaaca gtatcaggat
     8101 taaaggaaca actgatgtgg ttatcaggca aggcgacaaa actgcggtgg ttgttaaagc
     8161 tgatgataac ctaattaaaa atatcatcac aaaagtacaa ggggattttt tagttgttac
     8221 tgcagaagga agcatacgat cttataaaaa atttgaggta ttggttacca ttaatcgtct
     8281 tgaggcaatt gaggtttatg gcagtggtga tgttgaatca gagggaatca tcagcggaca
     8341 cgaacttagt attttagtta atggttcagg tgatgttgaa ttgaatcttg attttaaaaa
     8401 gttgacagct aatattaacg gctcaggcga catcgaagtc tcaggaataa atggcgatct
     8461 taacgcaaac attaatggct ctggcgattt tgaggcctcc tcagtgcgtt taaatgattg
     8521 taaaataagt gttaaggggg caggcaatgt aaaaatgcaa ggatcagcag caatagttat
     8581 gttaaatttg gctgcctcag gacgtattga actacaacga ctaatttctg aggatgtaaa
     8641 tgttcaaagc agtggctcgg gtaatgtata tgttaacgta aatggaagcc tcaatgcaag
     8701 actttcagga tcaggaaatt tgaaatattt gggaaatcca gtaagtgttg atgtactatc
     8761 aacaggttca ggatcggttt ataagtaata atgccgacag gaaattcaaa agcggcaatc
     8821 ttttgatgat taataaaagt tttgctttaa aaaaaattgc attccctgca gtagtccatt
     8881 atcgtcttaa gtaatccact gcaacaaagt aggggttttt gtagtcgggc tgcctcacaa
     8941 gttcaaagtc attagtggta aatgggtttt tgttttgaat aaaatcaaaa tggagccggg
     9001 ttcttttttg gggatctccg tatatccatg tttctatttc gcttttttta aaaacttctg
     9061 ttggagctcc aaagatcata taaatcattc ctcgatcagt tttccatccc tctttgtatg
     9121 aggtaaagaa tagattggcg tcttt
//
LOCUS       DAOOAI010000089         8810 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_9780_length_8810_cov_4.328726, whole genome shotgun sequence.
ACCESSION   DAOOAI010000089 DAOOAI010000000
VERSION     DAOOAI010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8810)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8810)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8810
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9780_length_8810_cov_4.328726"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="PK345_06980"
     CDS             complement(<1..398)
                     /locus_tag="PK345_06980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34794.1"
                     /translation="MKLKKPSISKLAIILVVLIVAGTINNLGRWKYDEKVIIHDIYAF
                     YAYLPATFIHHDLTLSFIEGTSYNNQFILWPKITDEGKKVFTSSMGMASLYFPFFLTA
                     HIYATTGAYPADGYSLPYRLALILSSFTFLV"
     gene            691..1551
                     /locus_tag="PK345_06985"
     CDS             691..1551
                     /locus_tag="PK345_06985"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HPX34795.1"
                     /translation="MSEYRFEELDLSPEGIREISGLLTSVFGREAGLAFEFLQWEYND
                     NPVGRAVGFNAWMGDELAAHYVTQPIISVFEGEQIRGLLSLNTATHKHHRGKKLFTML
                     AEKTYESGLSQGYDFVVGVANANSTPGFVKNLGFELISPLDVKFGIGRHNSVIAEDYD
                     YSRFWDKKSLAWRLSNPKLQYEILKQKEYSVIFAKTGKRGIHAILGTFLNDDLPDNIS
                     IKSKSKFSSINIWMGLDRQLRWQGRCYFNFPNRFKPSPLNLIFRDLTGKNRIPKPEQI
                     RFMALDFDAY"
     gene            complement(1548..3722)
                     /locus_tag="PK345_06990"
     CDS             complement(1548..3722)
                     /locus_tag="PK345_06990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HPX34796.1"
                     /translation="MFLGIAMGWFGFMPSFSELEDPQTYFASEIYSHDNELIGTYFIE
                     NRSKSHYSEISPDLVNALIATEDVRFYKHSGIDLKALLRVLYGTIMGKNKGGGSTITQ
                     QLAKNLFPREYNQSSLQLAFRKFKEWVIAIKLESRYSKDEIITMYLDKFDFLNLAVGI
                     KSASRVYFSKTPAELTTNQAALLVGMVQNPSLYNPVRRPELALQRRNIVLSQMEKYGY
                     LDKVVADSLKQEPLELEFQRVDHNVGIATYFKEFLRGQMMEWCSNNLKADGTPYDLYR
                     DGLKIYTTLDSRMQRYAEEAVYEHLGLNLQPAFFDHWKGHRYAPFVFGADEIKEQVEK
                     IMDQAMRRSDRYRSLKNDNVSMDSIKINFNTPVEMTIFSWKGDIDTIMTPMDSIRYFK
                     SFLRSGLMAVDPPTGEVRAYVGGPDYRYFKFDQVTQAKRQVGSTFKPFLYTLAMQEGA
                     ATGEFSPCTEVPNISVSIELPDGNYWTPRNSSRDREGEMVTLKWALANSVNWVSAYLM
                     KRFSPLAVIQMTERMGITSEIDPVPAIALGTPDISVFEMVGAMNTFANKGIHVNPYYI
                     THIKDKYGNVLATFAPTSNEAMNEETAYLMLNLMQGVVESGTGIRLRYKYGFNNEIAG
                     KTGTTQNQSDGWFMGIVPQLTTGVWVGCEDRSAHFRSISLGQGANMALPIWALFMQKV
                     YNDESLNIVKDPFPKPSSPLSVEIDCDSYNKDDSNNYEWDDF"
     gene            complement(4141..4635)
                     /locus_tag="PK345_06995"
     CDS             complement(4141..4635)
                     /locus_tag="PK345_06995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gliding motility lipoprotein GldH"
                     /protein_id="HPX34797.1"
                     /translation="MNRSFLKNNLTNPIILLFIYASICLVTSCDQNRFFDQSVKIPET
                     GWHLDSVAMFELEVMDTLGFYSFYINLRNTTNYTYSNFYLFLNTTLPDGEHARDTIEL
                     ILADKKGKWIGKGSGRLRDNQILIRPNLHFPLKGKYVFGIQHAMRDTILTDIKNIGIR
                     IEKW"
     gene            4964..6187
                     /gene="rocD"
                     /locus_tag="PK345_07000"
     CDS             4964..6187
                     /gene="rocD"
                     /locus_tag="PK345_07000"
                     /EC_number="2.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005846053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004587 - ornithine-oxo-acid transaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine--oxo-acid transaminase"
                     /protein_id="HPX34798.1"
                     /translation="MSEKITSQMAIEMEDKYGAHNYHPLPVVLSKGEGVFLWDAEGKR
                     YYDFLSAYSAVNQGHCHPKIIKALVDQAKTLTLTSRAFYNDCLGPYEKYVTSYFGYDK
                     VLPMNTGAEAVETAIKICRKWAYDVKGTPANEAKIIVCGGNFHGRTITIISMSTDPDA
                     RGGFGPYTPGFIVIPYNDIPALEKALQDPTVAGFLVEPIQGEAGVYVPEEGFLRKAYD
                     LCKAKNVLFIADEVQTGIARTGKLLACDHEGVRPDMLILGKAISGGVFPVSVVLADDP
                     IMMVIKPGQHGSTFGGNPIAAKVAIAALEVIKEEKLAERAEKLGKIFRDELKKIKSDM
                     IELIRGKGLLNAIVIKPKNGKEAWDVCLKMAELGLLAKPTHQHIIRFAPPLVITEEQL
                     YDAIEIIRKAILSFG"
     gene            complement(6412..7038)
                     /locus_tag="PK345_07005"
     CDS             complement(6412..7038)
                     /locus_tag="PK345_07005"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HPX34799.1"
                     /translation="MLQTKVKDLSFPSLPYAYDALEPHIDAMTMEIHHTKHHKTYFDR
                     FMAAVKGSDNENRSFGELISNISKLPAGVRNNGGGYLNHDLFWRIMKKNGGGQPEGNL
                     NKAITDAFGSFEKFKEMFSDAAANRFGSGWAWLIVDSSKNLKVTSTPNQDSPLMDIAE
                     VKGTPILALDVWEHAYYLKYQNRRPEYINAFWNVINWEEVEKYWKMTQ"
     gene            complement(7165..>8810)
                     /locus_tag="PK345_07010"
     CDS             complement(7165..>8810)
                     /locus_tag="PK345_07010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPX34800.1"
                     /translation="QINSRIEISVLGYLASNTFRFIPKTRETSFGTISEVKQLTIYFD
                     GGEMDKYQTMMGAAQLTYKPQKNVQLKFITSTYSSDEKETFDIKGQYYIGKVDTGLGS
                     ENFGETVGTIGIGTYLDHARNYLQSNVINIEHKGSYELSNKFFVWGVKYQNEQINDQL
                     KEWNLIDSAGYTLPRPPDQINIENPQVMPLDMDFLTQSFTKMNTNRVSGYVQNSWVLN
                     EDSNEFTLTGGIRANYWDYSGDLLISPRISVSYNPNWKHDLLFRFATGYYYQPPFYKE
                     LRDFKGNLVPLQDAQKSIHFVAGADWNLTIWNRPFKFTTEAYYKHLDDLIPYEVDNVR
                     IRYYADQKARGYAAGIDMKINGEFVPGIESWASMSIMKTEEDILGDYYFRYYDEDHQL
                     IGIGPEENSDAALNERVEPGFIPRPTDQRFRFSMFFQDYFPTNPTYKMHLALYFGSSL
                     PFGPPKSARYQQILRMPPYRRVDIGFSKQIIGERIYFPKNKPIMKISSLWLSLEVFNL
                     LQVSNTISYVWVTDVTGVKYAVPNYLTPRQLNIKLVAHF"
BASE COUNT         2643 a         1543 c         1712 g         2912 t
ORIGIN      
        1 acaaggaaag tgaaagaaga taatattagc gctaacctgt atggtaggct gtatccatcg
       61 gctggataag cgccggtagt agcatagata tgagctgtaa ggaaaaaagg aaaatagagg
      121 gatgccattc ccatagagga cgtgaacact tttttgcctt catcagttat ttttggccaa
      181 agaataaact gattattgta tgatgtgcct tcgatgaagg aaagtgttaa atcatggtgg
      241 ataaatgttg cgggtaaata ggcataaaaa gcgtaaatat catggataat caccttttca
      301 tcgtatttcc aacgaccaag gttatttata gttcctgcca ctattaaaac aactaaaata
      361 atagcaagct ttgaaatgga tggctttttt agcttcattg tttttttttg acaaagataa
      421 gatttaatgg agttttcaat gaattgataa gaaattatgt gagatattaa tgtgttgcgt
      481 aaacttgaga gtatttagtt acttttacag cgcggttagg tatctatgtt gaatgaatca
      541 ggctttcagt gtattattgt tttaatgcag attaatcatt tgataatact taatcggggt
      601 tgtcttcata aaatcaatag tttaacctaa tattgctttc tattgattaa ttcgggttta
      661 attttacggt gataccataa aataattgct atgagtgaat atcgttttga ggaacttgat
      721 ttgtccccgg aagggattag agaaatttct ggtcttttaa catcagtatt tggtcgtgaa
      781 gctggtttag cttttgagtt tttacagtgg gaatacaatg ataatcctgt tggaagggct
      841 gtaggtttta atgcatggat gggagatgaa ttggcggcac attatgttac tcagcctatt
      901 atttctgtat ttgaaggaga gcaaattcgt ggattgctat ctttaaatac ggctactcat
      961 aagcatcata gggggaaaaa gctttttaca atgcttgccg aaaaaactta tgaatctggc
     1021 ttatcacaag gatatgattt cgttgttggc gtggcaaatg ctaatagtac tccgggattt
     1081 gtaaaaaatt taggatttga gttaatcagc cctttagacg ttaaatttgg tattggccgg
     1141 cataattcag ttattgcaga agattatgat tactcacggt tttgggataa aaagagtctt
     1201 gcatggcggc tttcaaaccc taaacttcaa tatgaaattt taaaacaaaa agagtattct
     1261 gtaatatttg caaaaaccgg taaacgtggc attcacgcaa tattgggaac atttttgaat
     1321 gatgatttgc ccgataatat ctcaataaag tcgaaaagca agttcagttc aattaatatc
     1381 tggatgggat tggataggca attgagatgg caaggtaggt gttattttaa tttcccaaat
     1441 agatttaagc cgtcgccttt aaatcttatt tttagagatc ttactggtaa aaaccgtatt
     1501 ccgaaaccgg agcagatacg ttttatggct ttggattttg atgcctacta aaaatcatcc
     1561 cactcgtagt tgttagaatc atctttattg taactatcgc aatcaatttc aactgataat
     1621 ggggatgaag gcttgggaaa tgggtctttt acaatgttta atgattcgtc gttataaacc
     1681 ttttgcataa acaaagccca tattggcaat gccatattag cgccttgtcc taagctgatt
     1741 gaacgaaaat gtgcactacg gtcttcgcaa cccacccaaa cgcccgttgt aagctgaggg
     1801 acgatgccca taaaccaccc gtctgattga ttttgtgtag taccggtttt gccggcaatt
     1861 tcgttattaa atccgtattt gtaccttaat cgaattcctg ttcccgactc taccacgccc
     1921 tgcataaggt taagcatcaa ataggcagtc tcctcgttca ttgcttcgtt agaagttggt
     1981 gcaaaggtgg ctaacacatt tccatattta tctttaatat gggtaatata atatggattt
     2041 acgtggatgc ctttgtttgc gaaagtattc atagcgccta ccatttcaaa cacagaaata
     2101 tctggtgttc caagtgcgat agcaggaacg ggatcaattt cggaagtaat acccattctt
     2161 tctgtcattt gaataacagc taaaggggaa aacctcttca tcagataagc tgatacccag
     2221 tttactgagt ttgctagtgc ccatttcaaa gttaccattt caccctccct gtcgcgtgag
     2281 gagtttctgg gtgtccagta gttgccatca ggtaattcaa tacttactga aatattaggc
     2341 acttcggtgc aaggcgagaa ttcaccggtt gccgcgcctt cttgcattgc gagtgtgtat
     2401 aaaaagggtt taaaagttga gcctacctga cgtttagcct gtgttacttg gtcaaatttg
     2461 aagtaacgat aatcgggtcc gccaacgtat gcacgtactt cgccggtagg tggatcaacg
     2521 gccattagac cagagcgaag gaacgattta aaataacgga ttgaatccat aggagtcata
     2581 atagtatcaa tatcaccctt ccatgaaaat atcgtcatct ctactggtgt gttgaaattg
     2641 atttttatag aatccatcga gacgttgtca ttttttaggc tgcggtaacg atcgcttctg
     2701 cgcattgctt gatccataat tttttcaact tgttctttga tctcatcagc tccgaataca
     2761 aaaggagcat agcgatgacc tttccaatgg tcgaaaaatg caggttgtaa attgaggcca
     2821 agatgttcat aaaccgcttc ttcagcatat cgctgcatac gtgaatctaa ggtagtgtat
     2881 attttaagtc cgtcgcgata caagtcataa ggcgtaccat cagctttcaa gttgttgctg
     2941 caccactcca tcatttgacc tcggaggaat tctttaaaat aggttgctat tccaacgttg
     3001 tgatccacac gctggaattc caattcaagg ggttcttgtt ttagcgaatc agctactact
     3061 ttatcaagat aaccatattt ttccatctgc gagagtacaa tgttgcggcg ttgaagagct
     3121 aattcaggac gacgaactgg attataaagc gatggatttt gcaccattcc tacaagtagg
     3181 gcagcctgat ttgttgttag ctcagccggt gttttactga aataaactcg agaagcgctt
     3241 ttaataccaa cggcaaggtt cagaaaatcg aacttgtcaa gatacatcgt gatgatttca
     3301 tctttgctgt aacgcgattc taacttgata gcaataaccc attctttgaa ttttctgaaa
     3361 gcaagctgaa gagatgattg attatattct cgtgggaaca ggtttttagc aagttgttgc
     3421 gtaattgtac tccctccccc tttattcttg cccataattg tgccatagag cacacgcaac
     3481 aacgctttaa gatcaatacc cgagtgtttg taaaaacgaa catcttccgt ggctattaaa
     3541 gcattcacaa ggtcgggtga gatttcacta taatgacttt tgcttctatt ttcaatgaaa
     3601 taagtgccaa tcaattcatt atcatgcgaa taaatttcag atgcaaaata tgtttgagga
     3661 tcctcaagtt cgctaaagct tggcatgaat ccgaaccaac ccatcgcgat tcctaaaaac
     3721 atgataaaaa taccgaaaag accggcaata caatcttcca gaagtttttg atgtatttcc
     3781 ttgttttagc tttgtcccca aacttggtag tattacccat agattttgac ttaaatgaat
     3841 ttccttttaa acgaatttgc tttttatgac gcatagaatt aggtctatct tctttatcta
     3901 aaattattaa cttttattat cctcttttca tattaaggtg gtcgaaatat tatttttctc
     3961 ttttttcttt tatctatttc ttacatgttc taatcaagcc gtgtgttaat cctatgttaa
     4021 aaatactctc tattatcttt tttattataa cgaattataa atgttttgat atccattaag
     4081 tatcgtttac atgattgatt tgcggctttg ttttcagttg ttgattggaa aacttatgcc
     4141 tcaccatttt tcgatgcgaa ttcctatatt ttttatgtca gttaaaattg tatctcgcat
     4201 tgcatgctga attccgaaaa catattttcc tttcagaggg aaatgtaggt tgggtctgat
     4261 aagtatttga ttatccctca accttccgga gcctttccct atccattttc cttttttatc
     4321 tgccagaata agctctatgg tgtcgcgtgc atgctctcca tcagggagag ttgtgtttaa
     4381 aaagagataa aaattactat aggtatagtt tgttgtattt cttaggttaa tgtaaaaaga
     4441 ataaaaacca agtgtgtcca ttacctccaa ttcgaacatg gcaacagaat ctaaatgcca
     4501 gccagtttcc gggattttta ccgattggtc gaaaaatcta ttttggtcgc atgatgtaac
     4561 caaacagatt gaagcgtaga taaatagcag gataattgga ttggttaggt tatttttaag
     4621 aaacgatctg ttcactttaa ctatagtctg ttagtgttta atgctttggt tttttgcttt
     4681 tgaaagatca ctgaattaat ataaaaaatg agttgaatat tgaagaacaa aagtaaataa
     4741 agttttaaag ctgtttaaaa attcgcttta ggtattgccc aattcttatt attcttaaaa
     4801 tagaaacata ataaactact gtaataaaat gaatatcaat gttgtaagag tatgtgttgt
     4861 tagttgaata acacaagact taaaaacttc aacaaataat gatatttttg cacaaacttt
     4921 taattaaaat atttattaat cattaaaaat caaaaactta tttatgtcag aaaaaatcac
     4981 atcacagatg gccattgaga tggaggataa gtatggcgct cataattatc atccgttgcc
     5041 agtagtatta tcaaaagggg aaggcgtttt tctgtgggat gccgaaggca aacgttatta
     5101 cgattttctt tcagcttatt cagcagtgaa tcaggggcat tgccacccaa agattattaa
     5161 ggccttagtt gatcaggcaa aaacattaac acttacttct agggcattct ataacgactg
     5221 tttagggcct tacgagaaat atgttacttc ctattttggg tacgacaaag tattaccaat
     5281 gaataccgga gcagaagctg tggaaactgc tattaaaatt tgtcgtaaat gggcttatga
     5341 tgttaaagga actcctgcta atgaggcaaa gataatagtg tgtggcggaa attttcacgg
     5401 gcgcactatc acaattattt ctatgagtac cgaccctgat gctcgtgggg gctttggacc
     5461 atacacccct ggttttattg taatccctta caacgatatt ccagcgcttg agaaggcgct
     5521 tcaagatcct actgttgctg gctttttggt tgagcctatt caaggtgagg ccggagtata
     5581 tgtaccggaa gaaggatttt tacgtaaagc atatgatttg tgcaaagcaa agaatgtgtt
     5641 gtttatagct gatgaagtac agacagggat tgcacgtacc ggaaaattat tagcctgtga
     5701 ccatgaaggg gtacgtcctg atatgttaat tttaggtaaa gccatttccg gcggagtatt
     5761 tccggtttca gttgttttag ctgacgatcc gattatgatg gttattaagc ctggccaaca
     5821 tgggtctacc tttggcggta atcctattgc tgctaaggtt gctattgcag cacttgaagt
     5881 cattaaagaa gaaaaactcg ccgaacgtgc cgaaaagctg ggtaagatat ttagagacga
     5941 gcttaaaaag ataaaatcgg atatgattga gttgattcgt ggtaaaggat tacttaatgc
     6001 cattgttatt aagccaaaga atggaaaaga ggcttgggat gtttgtctta aaatggctga
     6061 attaggacta ttggcaaaac ctactcatca acatattatc cgttttgctc caccgttggt
     6121 aattaccgaa gagcaattgt atgatgctat agaaataata cgcaaagcta ttttaagttt
     6181 tggataaatt ttaattgtta tttcaaaaaa aaagccgcac ctaaaatagg gcggcttttt
     6241 ttaataacca aattatgtaa actatgaaat agttcttttg aattcgaaat aatcaataaa
     6301 aaacactttt gacctgaaca agtgatatta tgaaaccact acataccaag taactgttta
     6361 gtgcaattct tattgtacgg attactatca gttaaatggg gctaaaacaa tttactgtgt
     6421 cattttccaa tatttctcaa cttcttccca gttaattacg ttccagaatg catttatgta
     6481 ttcaggcctg cgattttgat atttaaggta ataggcatgt tcccaaacgt ctaatgctaa
     6541 tattggagtg ccttttactt ctgcaatatc cattaaagga gaatcctgat taggtgttga
     6601 agtaactttc aggtttttgg agctgtcaac tatcagccac gcccatccgc ttccaaaacg
     6661 attggcggct gcatcactga acatctcttt aaatttttca aaagaaccga acgcatcggt
     6721 tattgctttg ttaagattac cttcgggctg tcccccgcca tttttcttca taattctcca
     6781 aaaaagatca tggttaaggt aacctccacc attattacga acacctgctg ggagctttga
     6841 aatattagaa attaactctc cgaaggagcg attttcatta tcgctacctt taacggcagc
     6901 cataaatcta tcaaaataag tcttgtggtg ttttgtgtgg tgaatttcca ttgtcattgc
     6961 atcaatatgt ggctcaagag catcgtaagc atatggcaac gaaggaaatg ataagtcttt
     7021 tacttttgtc tgtaacataa tagatataag atttttttaa taatgtttta tatccaaaaa
     7081 acacaaaatc atctcttttg tttagagcga ggactgtttt ttttattttt taacaaaaaa
     7141 gatagtcaaa caaaaaggtt agaatcaaaa atgcgcaact agtttaatgt ttagttgccg
     7201 gggtgtaagg taatttggca ctgcatattt tacccctgtt acatctgtta cccaaacata
     7261 ggagattgtg ttgcttactt gcaacaggtt aaaaacttcc aaactcagcc aaagtgagct
     7321 tattttcata atgggtttgt ttttcggaaa gtaaatgcgt tcgccaatta tttgttttga
     7381 aaatccaata tctacacgtc ggtatggggg cattcttaat atttgctgat aacgggctga
     7441 ttttgggggg ccaaacggaa gactgcttcc aaaatataaa gcaagatgca ttttatatgt
     7501 cggattcgtt gggaagtaat cctgaaagaa catactaaat ctaaaacgct gatctgtagg
     7561 tcgagggata aatccaggtt caactctttc attcagggca gcatcagaat tttcttcagg
     7621 tcctattcca atcagttgat ggtcttcgtc gtaatagcgg aaataataat cccctaaaat
     7681 atcttcttct gttttcataa ttgacatact agcccagctt tcaattccgg ggacaaactc
     7741 tccgtttatt ttcatatcta tcccagcagc atatcctctg gctttctgat cagcataata
     7801 ccttatcctt acattatcca cttcatatgg tatcaggtca tccaaatgtt tgtaataggc
     7861 ttctgtagta aatttaaaag gcctgttcca gatggtaaga ttccaatcag cgccggccac
     7921 aaaatgaatt gatttttgcg catcttgtag aggaaccaaa tttcctttaa aatctcgcag
     7981 ctctttgtaa aagggcggtt ggtaataata acctgtagca aatcgaaata aaagatcgtg
     8041 tttccaatta ggattatatg agaccgaaat acgtgggctt atcaacagat ccccgctgta
     8101 atcccagtaa ttggctctga taccgcctgt taatgtaaat tcattgctat cttcgtttaa
     8161 aacccatgag ttttggacgt aaccagagac cctattggta ttcatttttg taaaggattg
     8221 tgttaggaaa tccatatcga gaggcatcac ctgtggattt tcaatgttta tttgatcagg
     8281 ggggcggggc aatgtgtagc cggctgaatc aatcagattc cactctttta gctgatcatt
     8341 aatttgttca ttttgatatt tgacacccca aacaaaaaat ttgtttgaaa gttcgtaaga
     8401 gcctttatgc tcaatattta ttacattaga ttgcaaatag tttctcgcat ggtccagata
     8461 ggtaccaata ccaattgtgc ctactgtttc tccgaaattc tcgctaccaa ggcctgtatc
     8521 aactttacct atataatatt gtcctttaat atcgaaagtc tctttttcat cactcgaata
     8581 agtggaggtg atgaatttaa gttgaacatt tttttggggt ttgtaagtca gttgcgcagc
     8641 gcccatcata gtctggtatt tatccatttc gcctccatcg aaataaatag tgagttgttt
     8701 gacttcacta atggtaccaa agctggtttc gcgtgttttt ggaataaatc ggaaagtatt
     8761 acttgcaaga tatcctaata cagagatttc gatgcggcta tttatttgat
//
LOCUS       DAOOAI010000090         8733 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_9898_length_8733_cov_5.125375, whole genome shotgun sequence.
ACCESSION   DAOOAI010000090 DAOOAI010000000
VERSION     DAOOAI010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8733)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8733)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8733
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9898_length_8733_cov_5.125375"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..375
                     /locus_tag="PK345_07015"
     CDS             <1..375
                     /locus_tag="PK345_07015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34801.1"
                     /translation="VTEEYKLTVNPAGQAPELYILGDATTVGWENTLALPMEGTGGLY
                     TITTELAGAGTLLKFIVTLGQWAPQYGTDENGTSTGGNLVYRPDDNTPDPNAIPAPDA
                     AGTYIVTANTNDLTYTIAPAKK"
     gene            complement(859..2973)
                     /locus_tag="PK345_07020"
     CDS             complement(859..2973)
                     /locus_tag="PK345_07020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007482423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HPX34802.1"
                     /translation="MKRAYIYVIAAFMIAFQGCQQPVSVKYPETKKVDVKDDYHGTII
                     EDPYRWLEDDKSEETAKWVEEQNKVTFGYLEQIPFREKIKNRLTELWDYPKYSVTFKK
                     GDRYFYFKNEGMQNQSVLYVQESLDAEPRLLLDPNTLSEDGTVALSNYSVSKDGKYLA
                     YGISRGGSDWNEIFVMEIESEKKLDDHLKWIKFSGISWKGDGFYYSRYDEPTGSELSS
                     KNEFHKVFYHEINTPQDQDILVFENKDFPLRNFGAGVTDDENFLIIYESESTSGNSLY
                     VKDFRKPNSVFEPIVENFDNDYSVIDHYDGKLLVLTNYDAPKYHLIEIDPSNKDKANW
                     KVIIPEQNEVLEGVTLVGGKIVTKYMKDAYSQAFIYDMTGNKIADLNLPGIGTLAGFS
                     GQKDDDIAFYGFTSFVYPSTIYKFDISTNESEVYRAPEIDIDPTAYETQQVFYESADG
                     TKIPMFIVHKRNLRRNGENPTLLYGYGGFNASLTPGFSATRLILLEQGGVFAMPNLRG
                     GGEYGKEWHEAGTKERKQNVFDDFIAAAEYLIEANYTSPDYLAIQGGSNGGLLVGAVM
                     TQRPELFKVALPAVGVLDMLRYHLFTIGWAWATDYGRSDVPEEFEYLIKYSPLHNLKF
                     GTCYPATLVTTADHDDRVVPAHSFKFIATLQEHKGCSNPTLIRIETKAGHGAGKPTSK
                     VIEEYADIYSFIFYNMRFTPVY"
     gene            complement(3503..4996)
                     /gene="nrfA"
                     /locus_tag="PK345_07025"
     CDS             complement(3503..4996)
                     /gene="nrfA"
                     /locus_tag="PK345_07025"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018108226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming cytochrome c nitrite reductase"
                     /protein_id="HPX34803.1"
                     /translation="MTKETSSQKKPWVNWAIFLVTVVIVFLIGLFASSIIERRSESQL
                     YFQMVSEIPDWEPRNEVWGQNFPREYESYVMTSDTSFKSKHFGSVTIDYLDKYPELVV
                     MWAGYAFARDYKQGRGHYYAIDDIRLTLRTIQPQPGTCWACKSTDVPRVMNEIGIAEF
                     YKKPWIELGAQIVNHIGCQDCHDPKTMNLRITRPGLIEAFERQGRNINDATHQEMRSL
                     VCAQCHVEYYFKGEGRYLTFPWDKGYGADEMEAYFDENAFADWEHALSKAPMLKAQHP
                     DYELYMTGVHADRGVSCADCHMPYQREGGLKFTNHHMGSPLNNISGSCQVCHRESEEK
                     LRQNVYDRQDKIAELRRVVEKNLARVHIEAKTAWDAGATNEEMTPVLQLIRQAQWRWD
                     WVAAANGMGIHSPVEALRVLGVSIQKSQEARVLLAEILIKHNIPIPITLPDISTKDKA
                     QQYINLPMDDLRIEKKKFFEEVVPEWIKEANERESKFQEKRIKISES"
     gene            complement(5015..5605)
                     /gene="nrfH"
                     /locus_tag="PK345_07030"
     CDS             complement(5015..5605)
                     /gene="nrfH"
                     /locus_tag="PK345_07030"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF015237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase small subunit"
                     /protein_id="HPX34804.1"
                     /translation="MKKILLFLIPPPQWRMAATIVLGIIVGLAFFIFHISKATSYLSD
                     KPETCINCHVMYPQYASWKNSSHHAVTNCTDCHVPNHNIFLKYYFKASDGARHATIFT
                     ARAEPQVISIKQAGANAVQQNCKRCHMDLVEMTSLVEHTYKSAHAGDGKLCWDCHREV
                     PHGSVRSLSSAPFSLVPRLPSITPDWLSASLKQNNK"
     gene            5864..7825
                     /locus_tag="PK345_07035"
     CDS             5864..7825
                     /locus_tag="PK345_07035"
                     /inference="COORDINATES: protein motif:HMM:NF025861.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 97 protein"
                     /protein_id="HPX34805.1"
                     /translation="MKHLSLLSFFFIFSISLCAQPFKVLSPDGSLTLTANTDKNISWS
                     VTYNGHTTIPNVNINLETNQGKLFDETTTVVNHTIQQHKKTLRVTVPVKSAEIEDYYN
                     ELTLYLSNGNHLVFRVYDDGLAYRFSTDFKDDVEIKNEILSIEFPAQTHCWFSAESSF
                     LSHFEQLFTYQRLDTIPSGQFHALPLLASTPEKVRIWISEADQHDYPALFLATKGNYQ
                     LNAIFPKYILQSEANPKHSPDRNEIILKEADYIAKTNGKRSLPWRIYTITNDDRKLVS
                     NQLVYQLSSPQAIDETDWIKPGKVAWDWYNANNLRKVDFKSGLNTPTYKYYIDFASKY
                     NLEYIILDEGWSQSTTEILTSNPDINLHEIIDYGKSKGVGVILWVLWKPLLSNLEEIL
                     NTYATWGVAGIKVDFMQRADQQMVNIFEEIAKVAAKNRLIVDFHGCFKPNGLQRTYPN
                     VLTFEGVKGNENHKWSAQITPEHNLTLPFTRMVAGPMDYTPGAMRNANRKNFAPSFER
                     PMSLGTRCHQLAMYIIYESPLQMLCDSPSEYLNEPEIPAFLSKIPTIWDQTIILDASV
                     SEFILSARRSGNNWYIAAMTNHFSRDLDIVLDFLPDGEYNMEIMSDGINADRHAEDYR
                     YEVKKVNNNTKMKIHLAAGGGFAAILTKE"
     gene            7848..8201
                     /locus_tag="PK345_07040"
     CDS             7848..8201
                     /locus_tag="PK345_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009859948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HPX34806.1"
                     /translation="MNIEDIRDYCLIKKGVTESTPFDDVTLTFKVAGKIFAFLPLDAI
                     TPTINLKCNPEWAVELREQYVAISPGYHMNKTHWNTVTLNGSISNRLIMEMIDHSYDL
                     VVNKLSKKQREMISE"
     gene            complement(8224..8436)
                     /locus_tag="PK345_07045"
     CDS             complement(8224..8436)
                     /locus_tag="PK345_07045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34807.1"
                     /translation="MKSGLFRYIPFANVVGIMLGGIGGYIYYLKVVCVTGGCPLTSNP
                     IITIVWGGALGYLFSDLFRKRKSKKE"
BASE COUNT         2700 a         1882 c         1561 g         2590 t
ORIGIN      
        1 gtaaccgaag aatacaaact taccgttaat ccggcagggc aagcccctga gctttatatt
       61 cttggcgatg ccacaactgt cggatgggaa aataccttag cgctgccaat ggaaggcaca
      121 ggaggactat ataccatcac caccgaattg gctggcgctg gaactttgct aaagttcatc
      181 gtaacattag gacaatgggc tccacaatac ggaactgatg aaaatggaac aagcacaggc
      241 ggcaaccttg tttatcgtcc tgacgataac acacccgacc caaatgcaat tccagcgcct
      301 gatgctgccg gtacatacat tgttacagca aacactaacg acttgactta caccattgca
      361 cccgcaaaaa agtaagtaga aaaatttatt aaaccccata aaaaaaggct acctcaaaac
      421 gagatagcct tttttattta tatccactca caatcagcca aaaagatacg atctttccat
      481 aacctgactt atccgggtaa atttaaagac taacattctc aaaagttgtt cagcctaaaa
      541 agcctgcaat tgattaagtc aggttattat tcgttatggt tgctgaataa agttaaagta
      601 ttcgtaactt aaattacttt taaaagcgga cttatttatg gtattggaac tacctaaata
      661 aaagcaatta ctcagcctga aatgactaac tactaatgca tttaggttta ataatattta
      721 tgctgaaact tattttggga aacagactga taatttaaaa aaaaacctga tgaacattaa
      781 ctgctcatca ggtttttata atcaaattta agggtttaag aataatattt ttatatcagt
      841 cttaatactc acccagtact aataaacggg agtaaatctc atattgtaaa aaatgaaaga
      901 gtatatatcg gcatactcct caataacttt cgatgtaggc tttcctgcgc catgtccggc
      961 ttttgtttca attcggataa gtgtaggatt actacagcct ttatgttcct gaagtgttgc
     1021 aataaattta aatgaatgag ccggcaccac gcgatcgtca tggtcggctg tagtaacaag
     1081 tgtagccgga taacaagttc cgaatttaag attatgcaat ggcgagtatt taatcagata
     1141 ttcaaactct tcgggaacgt cgcttcttcc gtagtcggta gcccaggccc accctattgt
     1201 aaacagatga tacctcaaca tgtcgaggac gcctaccgct ggcaatgcta ctttaaaaag
     1261 ctcagggcgt tgcgtcatca ctgcaccaac gagcagccct ccatttgatc ctccctggat
     1321 agcaagataa tcaggtgaag tatagttagc ttctatcaaa tattcggctg cagcgataaa
     1381 gtcgtcaaac acattttgtt tgcgctcttt ggtaccggct tcgtgccact ctttaccgta
     1441 ttcgcctcca ccacgcaggt tgggcatagc aaaaacgccg ccctgctcaa gaaggatcaa
     1501 ccttgttgca ctaaacccag gcgtaaggct ggcattgaaa cctccgtaac cataaagcaa
     1561 agttgggttt tctccatttc tccttaaatt tctcttatga acaataaaca tcgggatttt
     1621 cgtcccgtca gcgctttcat aaaacacttg ctgggtttcg taggctgtgg gatcaatatc
     1681 aatttcaggc gcccgataaa cctctgattc atttgtagat atatcaaact tataaatagt
     1741 tgacggataa acaaaagagg tgaatccgta aaaagcaata tcatcgtctt tctgcccgct
     1801 gaatcctgcc aaagtaccta taccgggcag attcaaatca gcgatcttat tgccggtcat
     1861 atcataaata aaagcctgac tataagcatc cttcatatat ttagttacaa tttttcctcc
     1921 tacaagcgtt accccttcaa gtacctcatt ttgctcagga atgataacct tccagttggc
     1981 tttgtcttta tttgatggat ctatttcaat caaatgatat ttaggtgcat cgtagttagt
     2041 caaaacaagt agtttgccat catagtggtc aattactgaa tagtcattat caaaattctc
     2101 tacaataggc tcgaacaccg aatttggttt tctaaaatct tttacataca aagaattgcc
     2161 agatgtactt tcggattcgt aaatgatcag gaagttttca tcgtcagtaa ctcctgcgcc
     2221 aaaattgcgt aaagggaaat ctttattttc aaaaaccaaa atatcctgat cctgcggagt
     2281 atttatttca tggtaaaaaa ctttgtgaaa ctcatttttc gacgacagct cgctacctgt
     2341 aggctcgtcg taacggctat aataaaaacc gtcacccttc cacgagatac ccgaaaattt
     2401 gatccattta aggtgatcat caagtttttt ttcactttca atctccatta caaaaatctc
     2461 attccagtcc gatcctcctc tcgaaattcc gtaggctaaa tattttccat cttttgacac
     2521 actataattc gacagcgcta cggtaccatc ctcagaaaga gtatttgggt caagcaacaa
     2581 tcttggctcg gcatccaggc tttcctgaac gtacaacaca ctttgatttt gcatcccttc
     2641 atttttaaaa taaaaatagc gatctccttt tttaaatgtt acgctatatt tgggatagtc
     2701 ccatagctcc gtaagccgat ttttaatttt ttctctaaaa gggatctgtt cgaggtatcc
     2761 aaaggtaact ttgttctgtt cctcaaccca tttggcggtc tcttcggatt tatcatcctc
     2821 aagccagcgg taaggatcct caatgatagt tccgtgataa tcgtctttta catcaacctt
     2881 tttagtttcc ggatatttta cgcttaccgg ctgctgacaa ccttgaaatg caatcataaa
     2941 cgctgcgata acataaatat aagctctttt catttgaatt ttatttggtg tttattgata
     3001 aatattcagt ttttcggaca aaaataaacc aaaaaaagaa tgtgtaaaat cttttttgca
     3061 gataaatcaa tagaaacaga atgacttatc aggcattaat aatcggttta acattttttt
     3121 acacccttta aatcattaat tatgaacctt tttacagcgc aacattaata tttaatacta
     3181 tgcccttcga ttttgatctt attcagaaag tttataaaca aataagtaaa aaatagatgc
     3241 agcccgaacg atgctcagcc gctctatgac attagctgaa aaaaatcctt tattcacgta
     3301 tctgggatag tttgccacaa acgaaacata agcgtataac cgactacgtt gactttgctc
     3361 ccgatcgtga tgaaatgcag gacaccatac ctgcatttgg ttttatcaaa aacttttcgc
     3421 gggttaaaat tatcttcttt tttttaacgg taacgtcaaa aaaaaatcag cataaaagag
     3481 ttttctaatg atcagtccgg gtttaacttt ccgatatttt tattcttttc tcttgaaatt
     3541 tactttcgcg ttcgtttgcc tcttttatcc attcaggcac cacttcttcg aaaaactttt
     3601 tcttttctat ccttagatca tccattggca gattgatata ttgctgggct ttatcttttg
     3661 tgctaatatc gggcaatgta ataggaattg ggatattgtg ttttatcaag atttctgcaa
     3721 gtaacactct cgcttcttgc gatttttgaa ttgaaacgcc taacacccgt aatgcctcca
     3781 ccggtgagtg gattcccatt ccatttgctg ctgccaccca atcccagcgc cattgtgcct
     3841 gacgtattaa ttgcagcact ggagtcatct cttcatttgt ggcgccggca tcccatgctg
     3901 tcttagcttc aatatggact cttgccaagt ttttctcgac aacacggcgc agctcggcaa
     3961 ttttatcctg acgatcatat acattttgcc gtaacttctc ttcactctca cggtgacata
     4021 cttggcacga tccggaaata ttattcaacg ggcttcccat atgatgattt gtaaatttca
     4081 aacctccctc acgctgatac ggcatatggc agtcggcaca tgaaacgcct cgatcggcat
     4141 gaacgccggt catataaagc tcgtaatcag gatgctgagc tttcagcatt ggggctttac
     4201 ttaaagcatg ttcccaatcg gcaaatgcat tttcgtcaaa ataagcctcc atttcatcag
     4261 cgccatagcc tttatcccaa ggaaaagtaa ggtaccttcc ttctcctttg aaataatact
     4321 ccacgtggca ttgtgcacaa actaacgagc gcatctcctg atgtgtggca tcgttgatgt
     4381 ttctcccttg cctctcaaaa gcttctatca acccagggcg ggtgatgcgt agattcatcg
     4441 tttttggatc atggcagtcc tgacaaccga tatgattcac tatctgcgcc cctaattcta
     4501 tccaaggttt tttataaaac tcagcaatgc cgatttcatt catcacacga ggcacatcag
     4561 tgcttttaca tgcccagcat gttcccggct gtggctgtat cgtccgcaac gtaagcctta
     4621 tatcatcaat agcataataa tgccctctgc cctgtttata atctctcgca aatgcatatc
     4681 cagcccacat aactactaat tcaggatatt tatcaaggta atctattgta acggatccga
     4741 aatgtttact cttaaaactt gtgtcggaag tcataacata ggactcatat tcgcggggaa
     4801 aattctgacc ccacacttcg ttgcgtggct cccaatcagg gatttcgctt accatctgga
     4861 aatagagctg cgattcgcta cgacgttcaa taattgatga ggcaaaaagc cctataagaa
     4921 aaacaataac tacggtaaca aggaatattg cccaatttac ccaaggtttc ttttgagatg
     4981 atgtttcttt tgtcattgat ataaatttta tttactattt attattctgt tttaaagaag
     5041 ctgatagcca atcaggagta atggaaggca accttggcac taacgaaaag ggagctgatg
     5101 aaaggctacg taccgatccg tgaggcactt cacgatgaca atcccaacac agctttccat
     5161 cacctgcatg tgccgattta taggtatgct caaccaagga tgtcatttca accaaatcca
     5221 tgtggcaacg cttacaattt tgctgcaccg cattagctcc agcctgtttt atactaatca
     5281 cctgtggctc agcacgcgcc gtaaaaattg tggcatgtcg agccccatcg ctcgctttaa
     5341 aataatattt tagaaaaata ttatggttag gcacatggca atcggtacaa ttagttactg
     5401 catgatgact actatttttc caagaagcgt actgaggata catcacatgg cagttaatac
     5461 aggtttcggg tttgtcggat agatatgaag ttgctttact aatatgaaaa ataaaaaaag
     5521 caagaccgac tatgatcccc aaaacaatag tagctgccat tcgccattga ggaggaggaa
     5581 taagaaaaag caggattttt ttcatctttt tgatattata aatcacaaac ttattatcaa
     5641 caaaaataac aggaaaaaca tctataatta caagagtatt aaatttttat tgattttaaa
     5701 taagccgaat taaatggctt aaatgtagtg aaaatcgaaa cacaaagctt gtcaaatggc
     5761 tgtattaacc cttattttgc aacaacctgc tccgtatctt caaatttaga gcaggaaaag
     5821 gcagttctgt tatctttgtc gttcaatctt aatttatcat ttgatgaaac atctatctct
     5881 gctttcattt ttttttattt tcagcatctc actttgtgcc caaccgttca aggttttatc
     5941 gcctgacggc tcattgacgt taaccgctaa cactgataaa aacataagct ggtcggttac
     6001 atacaacggg catacaacca taccaaatgt taatataaac ttagagacga atcaaggaaa
     6061 actctttgat gaaaccacaa cggttgtaaa ccatactatc cagcaacata agaaaacttt
     6121 acgggtaacg gttccggtga aatcggcgga aattgaggat tattacaatg agctgacact
     6181 ctatcttagc aatggcaacc atcttgtttt cagggtttac gatgatggat tagcttatcg
     6241 gttttcaacc gattttaaag atgacgtaga aattaaaaat gagattctga gcattgaatt
     6301 tcccgcacaa acgcactgct ggttttcggc tgagagttca ttcttatccc atttcgagca
     6361 gctatttact taccagcgtc tggatacaat tccatccggt caatttcatg cgcttcctct
     6421 actggcaagc acgccggaga aggtcagaat atggatatcc gaagccgatc agcatgatta
     6481 tcctgcattg tttttggcta ccaaaggaaa ttatcaatta aatgcaatat ttccaaaata
     6541 cattttacaa agcgaagcca acccaaagca ctcgcccgac aggaacgaaa tcatattaaa
     6601 agaggctgat tatatcgcaa agaccaatgg taaacgcagt ctcccctggc gcatctatac
     6661 aatcaccaat gacgatcgta agttagtatc taatcaattg gtttatcaac tgtcatcacc
     6721 acaagctatt gacgaaaccg attggattaa gcccggcaaa gtagcttggg actggtacaa
     6781 cgccaacaac ctgcgaaaag tggatttcaa atcggggcta aacacaccca cctataaata
     6841 ttatattgat tttgcctcga aatataacct tgaatacatt attctggatg aaggatggag
     6901 ccaatcaacc actgaaattc tcacctctaa ccccgatata aatctccatg aaatcattga
     6961 ttacgggaaa tcaaaaggtg taggcgttat cctttgggta ctttggaagc cgctactaag
     7021 caatttggaa gaaatcctca atacgtatgc aacgtggggt gtcgcaggga taaaggttga
     7081 ctttatgcaa cgcgccgacc agcaaatggt aaacatattt gaggagattg caaaagttgc
     7141 agcaaagaat agactcatag tagattttca tggctgtttc aagccaaacg gcttgcaacg
     7201 cacctatcca aatgtgctaa catttgaagg agtcaagggc aacgaaaatc ataaatggag
     7261 cgctcagatt acgccggaac ataacctcac cttgcccttc actcgtatgg ttgcaggccc
     7321 gatggattat accccgggcg ccatgcgtaa cgccaacaga aaaaattttg ccccaagctt
     7381 cgaacgccct atgagccttg gcacgcgttg ccaccaactt gccatgtata taatttacga
     7441 aagcccgctt cagatgctct gcgactctcc ttcggagtat cttaacgagc cggagatacc
     7501 ggctttcctt tccaaaattc caactatatg ggatcaaaca attattctcg atgcttcggt
     7561 ttctgaattt atcctgtcag caagacgaag tggtaacaac tggtacatcg cagctatgac
     7621 aaaccatttt tctcgcgact tagatatagt tctcgacttt ttgcccgatg gcgagtacaa
     7681 catggaaata atgagcgacg gtattaacgc cgaccgccat gcagaagact atcgttacga
     7741 agtaaaaaaa gtgaacaaca acacaaaaat gaaaatccat ttagccgcag gcggagggtt
     7801 tgcagccata ctaacaaaag agtgatcgga acctaaggaa cgaataaatg aacattgaag
     7861 atattagaga ctattgcctg attaagaaag gtgtaactga aagcactcct tttgatgacg
     7921 ttactttaac ttttaaggta gccggaaaaa tattcgcatt tttgccgctt gatgcaataa
     7981 caccaaccat taacctgaag tgcaaccccg aatgggctgt agagctacgt gaacaatatg
     8041 tagcaatttc tcctggatac cacatgaaca aaacacattg gaacacagta acacttaacg
     8101 gaagtataag caatagacta atcatggaga tgattgatca ctcctatgat ttggttgtaa
     8161 acaagttaag taagaaacaa cgggagatga tcagtgaata aaggctcttt gcgctctaaa
     8221 ggattactcc ttttttgact tgcgttttct gaagagatca gaaaaaagat agcctaaagc
     8281 gcccccccaa actattgtga taataggatt tgaagtgaga gggcagccac ctgtaacgca
     8341 tactaccttt aaatagtaaa tatatccacc gataccccct aacatgatcc caacaacatt
     8401 ggcaaaaggg atataacgaa aaagtcctga tttcatccac agatgttttt ttgaagtctc
     8461 ttcatccatc ttattaacag taaaaaggtt agtatgattt taacaattaa ttagctttaa
     8521 agttttcata actcgtttta gatttaggct tttctatttt atcaatacca attctgcgat
     8581 taataacttt actttcaaag atattattta taacttcggg aaactttcat ttattccatc
     8641 tttaagcaat ttatttacgt caataatcaa aattttctat ctttgatgtc ctaaacaaac
     8701 tatatgacaa tagatatttt tataggaatt gtg
//
LOCUS       DAOOAI010000091         8682 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_9975_length_8682_cov_5.669874, whole genome shotgun sequence.
ACCESSION   DAOOAI010000091 DAOOAI010000000
VERSION     DAOOAI010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8682)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8682)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8682
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9975_length_8682_cov_5.669874"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..903
                     /locus_tag="PK345_07050"
     CDS             <1..903
                     /locus_tag="PK345_07050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006282222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34808.1"
                     /translation="MLSPDNFIQAPDFSQSFNKYSYCLNNPLKYSDPSGEFFIIDSWL
                     IGLFSGGFKEANKWAWNDIKIWGGLFASDPNKGLLGRFWETISRFTWQLPQTIGGWGT
                     AQACNTLGLKGGVESVKYKYGATVVATQNSWDGAAITQVSYIVGGSELQADPNNSLFQ
                     HEYGHYIQSQSIGWAYYQRVGLPSAGSEHGKYKLNYPSHDYHPVEQDANRRAFLYFNK
                     HVTGFQNDTYLSDNLGWNFVKNPLDVYKTGHGIYIDYNNSYDLQLLNNLKVRATLGDY
                     ISWLYGGPIGAALYGWYNSYNYNN"
     gene            913..1311
                     /locus_tag="PK345_07055"
     CDS             913..1311
                     /locus_tag="PK345_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34809.1"
                     /translation="MRNFILILTAITLASVTCNREGESCHRKITIINHSTNSIVQALN
                     IKNAAGKCFLTGSILLPEEIYEYTNRSCFEDILSAGETFEFYLIDPNHYNTPGIFYDC
                     DSIEIKNTILKHYLLTLDELKSMNFTVNYP"
     gene            complement(1495..3306)
                     /locus_tag="PK345_07060"
     CDS             complement(1495..3306)
                     /locus_tag="PK345_07060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34810.1"
                     /translation="MTQSHNEAFVFGLIIFILWIYLVLRAILIQPMHDEIATFFFFVQ
                     SGRFIPYFSEWSTNNHLLNSALTHISFNLFGLSPIALRIPNLIVFPLYGFFIWKIGSY
                     IKYLPLRWGFFIATLFVHNYFEFFGLSRGYGMAFTSLVGSIWFTMLAFNTPKSKNIFF
                     ALLLGVLTVSAILININSLIVIFVLLIALIIYRGEGKRTKTITIILFLGLLPILLAVR
                     FLLDLNKAGSLNYGGNIGFWTTSVKDLTNMLFGSGASFVNIYIIILLIFLAAAIIHLF
                     YKKWSEGFTFDFLFKPQWVFFYLLIGNLLGFFIEHHLFGVLYPESRTMMQFVILFSGS
                     LFVILDNIEISKKAFLIIPVLPVFLLPIHFLGVSNFNKISVENETIPKRFLSFIEKRV
                     EESDLLPTIQGYKGRELRWAYLNYRERTFMPMVSCTTYPDTIADYQIIQPENYPYLNN
                     GYKLIDRDHVSGISILEREKKLKRELLIEREPEFSDEFTSDEFILISKGDGELANNSL
                     FFAEYEVNLQSKHKYPTVWIVVEIKDFDGNRLVYERVPLNWYNYNWKSGLDDFKTEIL
                     AITNDSLASEYITYLWNANCAPLKINKVKFSLHYLKT"
     gene            complement(3476..4573)
                     /locus_tag="PK345_07065"
     CDS             complement(3476..4573)
                     /locus_tag="PK345_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009161725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-deoxy-7-phosphoheptulonate
                     synthase/chorismate mutase type II"
                     /protein_id="HPX34811.1"
                     /translation="MSYSQYLLELSPIQSWLKPVTIPLVIGGPCSAESFKQVMDTARA
                     LFESGKVSVFRAGIWKPRSRPLGFQGAGEEGLKWLSKVKKTFKMPVAVEVAIPRHVEL
                     ADKYDIDILWIGARTVVNPFSVEELTKSLKGIERPVFVKNPINPDINLWIGAIERVNK
                     AGIKKIAAIHRGFQGLTRTKYRNEPMWEIPLELKRLFPEMPIITDPSHISGSRALLKD
                     ICQKSIDFQMDGLMIESHINPSKALTDIDQQITPAELSRIIDSLIIRKKRKTKSFNTL
                     DVLRNEIDSIDHELLGILAKRMEMAIKIGHYKKENNIDPIQPKHWKKIIEERISFGGS
                     SGLDEEFLRELFDLIHQESIKKQNDIIKKKD"
     gene            4651..6075
                     /locus_tag="PK345_07070"
     CDS             4651..6075
                     /locus_tag="PK345_07070"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family protein"
                     /protein_id="HPX34812.1"
                     /translation="MVFSSNLFLLYFLPSFLIVYYIIPRKLKNLFTLLASIFFYAWGA
                     PDFIFIVLAAIIIDFYIVDIMHKSTLLKAKRILLGISVLINIGLLAYFKYANFLVENF
                     NEILSLLGYEPINWASVALPIGISFFTFQKLTYSVDVYRKVHTPLKRVTDYAMYILMF
                     PQLIAGPIIRFNEIADQIENRRAFETIDAKLLGFFRFVVGLSKKVLIANVLGEQVDKI
                     FSMTPIDISTPLAWIGVVAYSFQIYYDFSGYSDMAIGIGKMIGFKFPENFNNPYISLN
                     ITEFWRRWHMTLGRWMRDYLYIPLGGNKVSKPRLYFNLWVVFLISGLWHGAAWNFVVW
                     GAFHGFFLIADRMFLIKLTKPLGKFTNIILTYFITLISWVLFRADTLGYAFQYIKRMF
                     AFNGVKTSLWLDAKFNTILVIGAIFAFIAAFGKIESWWMNVLNKPKSNTVTILLIFVS
                     VIFTMICIGAITSSSFNPFIYFRF"
     gene            6078..7856
                     /locus_tag="PK345_07075"
     CDS             6078..7856
                     /locus_tag="PK345_07075"
                     /inference="COORDINATES: protein motif:HMM:NF028132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34813.1"
                     /translation="MKFSVKHILLFALIALLTLPVFQHGTKLFNIRPLDGDFILSLRP
                     QYTWKTWMNGTFQSQFNNYLEDHIGFRSFFVRLNNQLDFFLFKKANAEGIVVGKNNML
                     FEYDYIRALNGRDFIGKSTIDKKLLRLKFLQKHFKENFDIDFLLILEPSKARTYPEYL
                     PKHYQEMKKTMSNYEYIGSRLNDLEIKHLDLNRLFINAKDTASYPVYPLYGTHWSEFT
                     MSFVADTLIQFFETMRNINMPGYKIEMVISDTLHPMDYDGGRTLNILLKLPHQPMAYP
                     VFTFDDNGNDKIRPMVLAVADSYYWNFFNTRIPLHLFANEAFWYFNAKVYPDFYYSEK
                     WTKDLNLQNEVEKQNIIILSITERFLYNMGWNFIDQLYDIYTPEYTGNLVYNYENAIR
                     LNADWFNNVLQKAEKEKMSLEKAIYKEAYYQAFVNEPETFLTWYGDDHFRSVISNDKN
                     WSSAVRTKASEAGITFEEQLTKDAEWVFEKEYPEIFKLNKLIANYKTQITKDSLWFAA
                     VSEKAQKYFMPVEEMLNLDAEYIARQEASKSFDKEERVQVYIQSIKEDPEWLEVVIKK
                     AAEQGKSIEEMIREDAIFMVDQELKK"
     gene            7934..>8682
                     /locus_tag="PK345_07080"
     CDS             7934..>8682
                     /locus_tag="PK345_07080"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HPX34814.1"
                     /translation="MKQELNLEKIVRQVVEVSGLVSIYLKEEIGLLKRESIETKGVHN
                     FVTYVDRTSEEKIVAALNLILPQAGIIAEEMGDVKRQKRYNWVIDPLDGTTNYIHGLP
                     LYSISIALLDYDEVILGVVHEVNLNECFYAWKGSPAFLNGNRIMVSQSQKLNDSLIAT
                     GFPYHDYSMMESYLKAFGHLMRVSHGLRRLGSAAVDLAYVACGRFDGFYEYGLNLWDV
                     AAGQLIVKQAGGEISDFKGGKNYLLGKEMVAT"
BASE COUNT         2816 a         1544 c         1609 g         2713 t
ORIGIN      
        1 atgctctccc ccgacaactt catacaagcg cccgatttct ctcaaagttt taacaagtat
       61 agctattgcc tcaataaccc gttgaaatat tcagatccaa gcggggaatt ctttataata
      121 gactcatggc ttataggttt atttagcgga ggatttaagg aggcaaataa atgggcttgg
      181 aatgatatta aaatatgggg tggattattt gcaagtgatc ctaataaggg ccttttgggc
      241 cgattttggg aaaccatctc aaggttcaca tggcagctac cacaaaccat tggtggctgg
      301 ggtactgcac aggcgtgtaa tacattaggc cttaaaggtg gcgttgaatc cgttaagtat
      361 aaatatggtg caacagttgt tgccacacaa aatagttggg atggggcggc gataacacaa
      421 gtaagctata ttgttggagg gagtgagtta caagctgatc ctaataatag tctttttcaa
      481 catgaatatg gacactatat tcaaagccaa agcattggct gggcctatta tcaaagagtt
      541 gggctaccga gtgctggaag tgaacatgga aaatataaat taaattaccc aagccatgat
      601 taccatccag tagaacaaga tgctaataga agagcctttc tatattttaa taaacatgta
      661 actggattcc agaatgatac ctatttgagt gataatttag gatggaattt tgtaaaaaat
      721 cctctagatg tttacaaaac aggacatggt atttatatag attacaataa ttcttatgat
      781 ttacagctct taaataatct aaaagtaaga gctactcttg gtgattatat tagctggctt
      841 tatggaggcc cgataggcgc agctttatat ggatggtata attcatacaa ttacaataat
      901 taaatcagcc ccatgagaaa cttcatttta atcttaaccg caataaccct cgcttcagtt
      961 acatgtaatc gtgaagggga aagttgtcat cgcaaaataa cgattatcaa tcattcaaca
     1021 aatagcatag tccaagcatt gaatataaag aatgcagcag ggaaatgttt tttgactggt
     1081 tcaatccttc ttccagaaga gatttatgaa tatactaaca gaagctgctt tgaagatatc
     1141 ctttcagctg gcgaaacatt tgaattttat ttaatagatc ctaatcatta caatactcct
     1201 ggaatttttt acgattgcga ctcaattgaa ataaaaaaca ctatactcaa gcattatttg
     1261 ttaacgcttg atgaattaaa gagtatgaat ttcactgtta attatccttg atatttaggc
     1321 agtaattatt taaaacaatg aagccggatt aatccggctt cattgttaca ttagtcaatc
     1381 cttgacaact cactttcagt gaaggaatat aagacatctg tattagttta aaagcaaaag
     1441 tgggtgtgga gaattttttg aagtagaaaa aagaaaaggt ggtatctcat aatatcaagt
     1501 tttcaaataa tgcaatgaga attttacttt attaattttc aacggggcac agttggcgtt
     1561 ccataaatag gtaatatatt ctgatgctaa cgaatcgttg gtaatggcaa gaatctcggt
     1621 tttaaagtcg tctaaacctg atttccaatt atagttgtac cagtttagag gcactcgctc
     1681 ataaaccaac cgattaccat caaaatcttt aatctctact acaatccaaa ctgtagggta
     1741 tttatgtttt gattgaagat tgacttcata ttccgcaaaa aaaagactat tgtttgccaa
     1801 ttcaccatct cccttgctga ttagtataaa ttcgtcagat gtaaactcat cggaaaattc
     1861 aggctcccgc tcaataagta attctcgttt taattttttt tcccgttcta aaatagagat
     1921 acctgaaaca tggtcacggt caattaattt atagccatta tttaaataag gatagttttc
     1981 aggttgtatt atttgataat cggcaattgt atctggatag gtggtacatg aaaccatcgg
     2041 cataaaagtt ctttcacggt aatttaaata cgcccaacga agctctctac ctttatatcc
     2101 ctgaattgta ggaagtaagt ccgactcttc cactcttttt tcaataaatg acaaaaagcg
     2161 tttcgggata gtttcgttct caacagaaat cttgttgaaa ttggaaactc ctaaaaaatg
     2221 aatgggtaaa aggaaaacag gtaaaacagg aataataaga aatgcttttt tggaaatttc
     2281 aatattgtca agaataacaa atagagaacc tgaaaataaa attacgaatt gcatcattgt
     2341 gcggctttcc ggataaagca cgccaaagag atgatgctca ataaaaaagc caagcaagtt
     2401 gccaatgagt aaataaaaaa acacccattg aggtttaaac aaaaaatcaa atgtaaagcc
     2461 ctccgaccat tttttgtaaa acaggtggat gattgctgct gccaaaaaaa ttagcaatat
     2521 gataatgtaa atatttacaa agctcgctcc acttccaaaa agcatattgg tgaggtcttt
     2581 tacagatgtt gtccaaaacc cgatgtttcc accataattt aaacttccgg ctttatttag
     2641 gtccaacaaa aagcgaacag ctaacaaaat aggtagcaag cctaaaaata aaataattgt
     2701 aatggttttt gtcctttttc cttcgccacg ataaataata agagcgataa gcaaaacaaa
     2761 aattacaatc aacgaattga tgtttatcaa aatggcgcta accgttaaaa ccccaagtag
     2821 aagagcaaaa aatatgtttt tcgattttgg agtattgaaa gccaacattg tgaaccagat
     2881 gctgccgaca agggaagtaa atgccattcc ataacctctt gataagccaa aaaactcaaa
     2941 atagttatgt acaaataaag tcgctataaa aaacccccaa cgtaaaggca gatatttaat
     3001 gtaacttcct atcttccaaa taaaaaatcc gtaaagcgga aaaacaataa ggtttggaat
     3061 acgtaaagct atcggactta aaccaaaaag attaaaactg atatgggtta gcgccgagtt
     3121 gagcagatga ttgttggttg accattcgct aaaatatgga atgaaccggc ctgactgaac
     3181 gaaaaaaaag aaggtggcaa tctcatcatg catgggctga ataagtatag cccttaaaac
     3241 aagataaatc caaagaataa aaataatcaa tccaaagaca aaagcttcat tgtgtgattg
     3301 tgtcattgca gttttcaaaa aattggagct gccctgaaaa ttaagttttt tagttattcg
     3361 tgtatctaac atttcaacat gttatggaat atcaatgatt tatatagtta ttatttgtat
     3421 gtgatcgccg aacaaagttg aagtattcgc tgctaaaatt actcctcggc gttggctaat
     3481 cctttttttt gataatatcg ttttgttttt ttatggattc ctgatggata agatcaaata
     3541 attctcttaa aaactcctca tcaagccctg atgacccccc aaaagagatc cgttcttcga
     3601 ttattttttt ccaatgtttt ggctgtattg ggtcaatatt gttttccttt ttgtaatgtc
     3661 caattttaat agccatttcc attctttttg ccaaaatccc aagcagctca tgatcaattg
     3721 agtcaatttc attacgcaag acatcgagtg tattaaagga ttttgttttt cttttttttc
     3781 tgatgatcag gctgtcaatt atccgactaa gttcagctgg tgtaatttgt tggtcaatat
     3841 ctgtcagagc ttttgacggg ttaatatggg attctatcat cagcccgtcc atttggaagt
     3901 caattgattt ttgacaaatg tctttcaata gagctcttga accggaaata tgagacggat
     3961 cggtaattat tggcatttca ggaaataatc ttttcaattc taaaggtatt tcccacatcg
     4021 gttcatttcg gtatttagtc cttgtaagtc cttgaaatcc ccgatgaatg gcagctattt
     4081 tttttattcc cgctttatta accctttcaa tagctcctat ccatagatta atatcgggat
     4141 ttattggatt tttaacaaaa accggcctct ctatgccttt taacgatttg gtaagctcct
     4201 ctaccgaaaa ggggttaacc actgtgcggg cgccaatcca taatatgtca atatcatatt
     4261 tatcagccag ctcaacatgg cgtggtattg ccacctcaac agctacgggc attttaaaag
     4321 tcttctttac cttactcaac catttaagtc cctcttcgcc tgccccctga aagcccagcg
     4381 gacgtgaacg cggcttccag ataccggctc tgaacaccga cacctttccg ctctcaaaaa
     4441 gcgccctggc agtgtccata acttgcttaa aactctctgc actgcaaggc ccaccgataa
     4501 ctaatggaat agttaccggc tttagccacg attggatcgg tgaaagttct aataaatatt
     4561 gtgaataact cattggagca aaattatgct ttttgatgaa tctgattcac gattatctaa
     4621 tacatttgca atcattttac aattatcaaa atggtattca gtagtaatct atttttactc
     4681 tattttcttc cttctttcct gatagtttat tatattattc cgcgcaaact aaagaatctc
     4741 ttcactctat tagcaagtat ctttttttat gcatggggtg cgcccgactt catctttatt
     4801 gtattagcag ccattataat tgatttttat attgttgata tcatgcataa atctaccctg
     4861 cttaaagcaa aaaggatttt gttagggatt tccgtattaa taaatatcgg gttgcttgcc
     4921 tatttcaaat acgcaaattt tcttgttgaa aattttaatg agattctttc cttgttgggt
     4981 tatgagccta ttaattgggc ttcggtagcg cttcctatcg gaatatcgtt tttcactttt
     5041 caaaagttaa cctattccgt tgatgtttat cgcaaagtgc atacaccgct aaaacgcgtt
     5101 accgattatg caatgtatat tctcatgttt ccacaactga tagccgggcc tatcatacgg
     5161 ttcaacgaaa tagccgacca gatagagaat cgtagagcct ttgaaacgat tgatgccaaa
     5221 ttacttggtt ttttcaggtt tgttgttgga ttatccaaaa aagtactaat agccaacgta
     5281 ttgggtgagc aggtggataa aattttttcc atgaccccaa tagatatatc aacacctttg
     5341 gcatggatag gagttgtggc ttactcattt cagatctatt acgatttttc aggttactcc
     5401 gatatggcaa tcggtatcgg taaaatgatt ggttttaaat tccctgaaaa ttttaacaac
     5461 ccatatatat cgcttaatat tacagagttc tggcggcgct ggcatatgac tctcgggcgt
     5521 tggatgcgag attatctgta tattccgctt ggcggcaata aagtaagcaa accacggctc
     5581 tatttcaact tatgggtagt ctttttaatt tccggacttt ggcatggcgc agcgtggaat
     5641 tttgttgtat ggggcgcttt tcacgggttt ttcctgatcg ccgaccgcat gttccttata
     5701 aaattaacca aaccgcttgg taagtttaca aacataatac ttacctactt tatcacatta
     5761 ataagctggg tacttttcag agccgataca ctgggatacg cttttcagta tatcaaaaga
     5821 atgtttgctt tcaatggtgt caaaacaagc ttatggttgg atgctaagtt taatacaatt
     5881 ttggtaatcg gagcaatatt cgcttttatt gctgcatttg gaaagataga atcatggtgg
     5941 atgaatgtgc tcaataagcc caaaagcaac actgttacaa tattattgat attcgtttcg
     6001 gtaatattta ctatgatatg tattggcgca attacctcat cttcgtttaa tccattcatc
     6061 tatttcagat tttgataatg aaattttcag ttaaacatat tctcttattc gcacttatcg
     6121 cacttttaac tttgccggtt tttcagcacg gaacaaaact ctttaatatt cgccctttag
     6181 atggcgattt cattctatcg ctcaggccgc aatacacctg gaaaacatgg atgaatggta
     6241 ctttccaaag tcagttcaat aactatttag aagaccatat cggtttcaga tcgttttttg
     6301 taagattaaa taaccaactc gatttctttt tatttaaaaa agcaaatgcc gaaggtatcg
     6361 tggtaggtaa aaacaacatg ctttttgaat atgattacat aagagcctta aatggccgcg
     6421 attttatcgg taaatcaacc attgataaaa aactactacg attaaagttt ttacaaaaac
     6481 acttcaaaga aaatttcgat atagatttct tgctaatcct cgagccgagc aaagcccgaa
     6541 cttacccgga ataccttcca aagcattatc aggaaatgaa aaagaccatg tcgaattatg
     6601 aatatattgg aagtcggctt aatgatttag agataaaaca ccttgatttg aatagattat
     6661 ttatcaatgc aaaggacacg gcctcatatc cggtttatcc gttatacggt acccattgga
     6721 gcgaatttac aatgtcattt gttgccgata ctctcatcca gttttttgaa accatgcgca
     6781 atattaatat gccaggatat aaaatagaaa tggtgataag cgacactttg caccccatgg
     6841 attatgatgg aggaagaaca ttgaacatac ttctgaagct ccctcatcaa cctatggcct
     6901 atcctgtttt tacatttgac gacaatggta atgataaaat aagacccatg gtgcttgcag
     6961 ttgccgatag ttattactgg aactttttta ataccagaat accattacat ctctttgcaa
     7021 acgaagcctt ctggtacttt aacgctaaag tttatcccga cttttattat agcgaaaaat
     7081 ggactaaaga cctcaaccta caaaacgagg tagaaaaaca aaacatcatt atacttagca
     7141 tcaccgaacg atttctttat aacatgggat ggaattttat tgatcagctt tacgacattt
     7201 acacccccga atatactggc aatcttgttt acaactacga aaacgcgatt agactaaatg
     7261 ctgattggtt taataacgtg ctgcaaaaag ctgaaaaaga gaagatgtct ttagaaaagg
     7321 caatttataa agaagcctat tatcaagcat ttgttaatga accggagaca tttttgacat
     7381 ggtacggaga cgatcatttt cgctcggtta tttcgaatga taagaattgg agttcagcag
     7441 taagaacaaa agcatccgaa gcgggtatta cattcgaaga gcaattgact aaagatgctg
     7501 aatgggtatt cgaaaaagaa tatcctgaga tttttaaact caacaaacta atagccaatt
     7561 acaaaacgca aataacaaag gattcattat ggtttgccgc cgtgtcggaa aaagcacaga
     7621 aatatttcat gccggtggaa gagatgctaa atttagatgc cgagtatatt gcaaggcagg
     7681 aggcatccaa aagttttgac aaggaggaaa gggtccaggt gtatattcaa tccataaaag
     7741 aggatcccga atggttggag gttgtcataa aaaaagctgc cgaacaaggc aaatccattg
     7801 aagaaatgat tcgtgaagat gcaatcttta tggttgatca agaattaaag aaatagatgc
     7861 gtttagttac attttaataa ttcccttatc ttattaaaag aaattagtta tttttaggaa
     7921 aatcaatatt tgtatgaaac aagagctaaa ccttgaaaag attgtacggc aggtcgtcga
     7981 agttagcggc ttggtaagta tttatcttaa agaagaaatt ggattgttaa aaagagaatc
     8041 tattgaaacc aaaggggttc ataactttgt tacttatgtt gacaggactt ccgaagaaaa
     8101 aattgtagct gctttaaatt taatacttcc ccaagccggc ataattgccg aagagatggg
     8161 ggatgtaaaa cgacaaaagc ggtataattg ggtaatagat ccgcttgatg gaacaacaaa
     8221 ctacattcat ggattaccac tttactccat cagcatcgct ttattagatt atgatgaagt
     8281 gatattgggc gttgttcatg aagtaaacct gaacgaatgt ttttacgcat ggaaagggag
     8341 ccctgcattt ttaaacggaa ataggataat ggtcagtcaa tcgcaaaagc taaatgatag
     8401 ccttatcgcc acgggttttc catatcacga ttattccatg atggaatcgt atctaaaggc
     8461 tttcggacat cttatgagag ttagccatgg gcttcgcagg cttggttctg ccgccgtgga
     8521 tcttgcctat gtggcatgcg gacggtttga tgggttttac gaatatggcc ttaacctctg
     8581 ggatgtggct gccgggcaac tgattgtgaa acaagccgga ggtgagattt cagattttaa
     8641 aggcggtaaa aattatttgt tagggaaaga aatggtagca ac
//
LOCUS       DAOOAI010000092         8389 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_10352_length_8389_cov_4.534077, whole genome shotgun sequence.
ACCESSION   DAOOAI010000092 DAOOAI010000000
VERSION     DAOOAI010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8389
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10352_length_8389_cov_4.534077"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            104..2893
                     /gene="ppdK"
                     /locus_tag="PK345_07085"
     CDS             104..2893
                     /gene="ppdK"
                     /locus_tag="PK345_07085"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018668497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HPX34815.1"
                     /translation="MSKDKLKYVYLFGDGKAEGDATMKNLLGGKGANLAEMNLIGIPV
                     PPGFTVTTEACTIYNEQGRDTVVKLIEDEVHKAVKNLEKIMGTKFGDSKNPLLLSVRS
                     GARASMPGMMDTVLNLGLNDKAVEGLALKTGNDRFAWDSYRRFVQMFGDVVMGLKPES
                     IFEEDPFEVIIHQLKEERKVEKDSDLTTDDLKELVKRFKATIKEKTGKDFPTDPWEQL
                     WGSIMAVFDSWMNKRAITYRKLNNIPASWGTAVNVQAMVFGNMGNHSATGVAFTRDSA
                     TGEKLFNGEYLVNAQGEDVVAGIRTPQQITLEGSKRWAKLQGISEQMRAASFPSLEEL
                     MPKAYNELFKYQRKLENHYKDMQDLEFTIQEDKLWLLQTRSGKRTGMAMVKIAMDLYR
                     ENKISKNEALLMIEPNRLNELLHPVFDKAVLQKANVIAKGLPASPGAASGQIVFNAEE
                     AQMWANQGKKVILVRIETSPEDLGGMNVAEGILTARGGMTSHAAVVARGMGKCCVSGA
                     GSLKINYKNRTVKTDNANLKEGDWISLNGTTGEIYEGKIGTIDPELSGDFNRLMEIAD
                     SIAKMEVRTNADTPKDALKARNFGATGIGLCRTEHMFFEGNRITAMREMILADDEEGR
                     RKALDKLLPIQRSDFEGIFEAMHDLPVTVRLLDPPLHEFVPHDDKEQEEMAKVMNIDV
                     SIVKAKVEALHEINPMLGHRGCRLGNTYPEISEIQSRAIIEAALNLKKRGIVTKPEIM
                     IPLTGSLSEMKMQEDIVRETVEKVFEERKDTIEYMVGTMIEVPRAALLADEIAQSAEF
                     FSFGTNDLTQMTYGYSRDDAGKFLGVYLDKGILPNDPFQILDQEGVGQLMRVAVKRGR
                     DARKNLKIGICGEHGGEPSSVEFCHTLGFDCVSCSPFRVPIARLSAAQAVINEKMAKK
                     AGKVTKNKKKKKTKK"
     gene            complement(3450..4793)
                     /locus_tag="PK345_07090"
     CDS             complement(3450..4793)
                     /locus_tag="PK345_07090"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HPX34816.1"
                     /translation="MSMKRVLILGAGLVVKPIVAYLLERGYYVTVATRTKSKADNMIG
                     GHKNGKSIAWTVDDEPGLNDMIKDHDLIVSLLPWAYHVIVAKICITHKKNMVTTSYVK
                     PPMQALDSEAKKAGIIILNELGLDPGIDHMSAMRIIDYVHNKKGKIEEFYSLCGALPA
                     PEACDNPFNYKFSWSPKGVVMAGNNDGHFLKRGKEIYIEPIDIFKNPFIVDFPDVGEL
                     EVYPNRDSIPYINIYGIPETKTIFRGTFRFPRWCETLDAMKRLHLITSDEYDFTNLTY
                     AGFVAKLIGEKDPTDIRKKAAKYINLPADSYAFDAMEWLGLFDDKPMGRGKDTPYEIT
                     SDMMISKMMLGYNERDMVVMQHTFLASYPDGSKEVIKSRMLDFGTPATDTAIARTVAL
                     PAAVGVEMILEGKINARGVHIPVIPEIYNPILDSLEALDIRMIEEYGLPESENIQ"
     gene            complement(4824..6131)
                     /locus_tag="PK345_07095"
     CDS             complement(4824..6131)
                     /locus_tag="PK345_07095"
                     /inference="COORDINATES: protein motif:HMM:NF017072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysine ketoglutarate reductase
                     /saccharopine dehydrogenase family protein"
                     /protein_id="HPX34817.1"
                     /translation="MNRKVGIRHEDKYLMERRSALTPRHVKKLVGHGIEVHVERSKKR
                     VFHENEYKHAGAVITDNLSEIPVIFGVKEIPASWFEEEKTYIFFSHVIKGQPYNMPML
                     SEMMGKRCNLIDYERIVDDSGRRLIFFGRFAGLAGMINSLWSFGARMKVLGVETPFLR
                     IKQSVNYHSLADAEQAIREVGELIRAQGLPDEMAPFTIGFTGRGNVSKGSQHIANLLP
                     AVEITPAELINLKASGNYSNKLIYKTVFYKDDITEPIEPGKPFSLKEFHQHPENYKNS
                     FQKYLPHLTILMNCMYWDENFPRLVSKDDIEQLYKKGDMKLKVIGDVTCDPDGSIEFT
                     HKGTAIEDPVFVYDPVNRTPTMGFKGNGILVMAVDILPSELPRDSSEAFGDALIDFIP
                     EIVRANYNVSFEKLELPPEIKRALILHKGKLTPDYQYLQKYLD"
     gene            6780..>8389
                     /locus_tag="PK345_07100"
     CDS             6780..>8389
                     /locus_tag="PK345_07100"
                     /inference="COORDINATES: protein motif:HMM:NF013508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPX34818.1"
                     /translation="MCFKRITPSIPFSMSCTALFGFQFKRNHSSYWLSAVLISFFLMI
                     TAIGFAQAPAPVWHTSGLKTNFGIGTAVSVNRPDAVVAGDLILLFFAQTKSSMIDSIS
                     GFTTPTGFTMIHSEHNTTTNLRPEVVAFYKIAGGSEPAAYTSTATDFLSTAPMWKAVA
                     VRVTGHDPTNPIQGHSGNSPTLATTSVTLPSLTTVVSNTLLVSAVTVRRSISSINVNP
                     MTLQWFLDGTGTTDDSSNEPSLFGSTQVIPSAGSTGTRQLTWSGSGRAAGLMFTINPS
                     LVNADLEVTMSVNPSSSHYFADTVNYTITATNLSNSLNGATGVVVTDLLPPGLGLTSH
                     TASTGTTYTPATGKWNIGNLAKNESKTLTLQVTIVCFETNFKNVATIIGNEFDPNILN
                     NQAEITLSPMTGGSNPCPIIANDDIANGLQGEPIIIHVTNNDKGEFDVSTLSITVQPI
                     NGTTQIVQNGIIHYLPNGNFTGTDQFTYRICNTAETICDVATVYVTITEDYTSPCLEA
                     TRDKLFYLPFPENKDQMREALLSAGSYY"
BASE COUNT         2458 a         1559 c         1799 g         2573 t
ORIGIN      
        1 taccttaata agtgcatcaa aaattttatt tttgcgtcag ttttttaata ttatcaattt
       61 gaatatcaat ttaaaagtaa taaacttaat attccattaa tttatgagta aagacaagtt
      121 gaagtatgtt tacctttttg gtgatggcaa agctgaaggc gatgcaacga tgaaaaatct
      181 tcttggtggt aaaggagcta accttgcaga gatgaatctg ataggcatac ctgtaccgcc
      241 gggatttact gtaacaactg aagcgtgcac aatttataat gagcagggac gcgataccgt
      301 agttaagctc atcgaggatg aggtgcataa ggcagtcaag aacttagaga agatcatggg
      361 aacaaagttt ggcgattcca aaaatccgtt gttactctct gttcgttcgg gggcaagggc
      421 atccatgcct ggtatgatgg acactgtgct taatttgggt ttgaatgata aagctgttga
      481 aggacttgca ttgaaaaccg gtaatgatcg ttttgcatgg gattcctacc gtcgttttgt
      541 tcagatgttt ggcgatgtgg taatgggact taaaccggaa tccatttttg aagaagaccc
      601 ctttgaagtg ataatacatc aattaaaaga agaacgtaag gttgaaaaag actctgattt
      661 gaccactgat gatctgaagg agctggttaa aaggtttaaa gctaccataa aggaaaaaac
      721 cggtaaagat tttcctaccg atccatggga gcagttatgg ggttctataa tggctgtatt
      781 tgatagctgg atgaataagc gtgctatcac ttatcgcaag cttaataata ttccggcttc
      841 ctggggtact gccgtgaatg ttcaggccat ggtttttggt aatatgggta atcattcggc
      901 taccggtgtt gcttttacac gcgactcagc taccggtgaa aaactcttta atggcgaata
      961 tcttgttaat gctcagggtg aagacgtggt ggcaggtatc agaacgccac agcaaatcac
     1021 acttgaaggt tcaaaaaggt gggctaaact gcagggtatt agtgagcaga tgcgcgccgc
     1081 ctcttttcct tcgcttgaag agttgatgcc gaaagcttac aacgagctgt ttaaatatca
     1141 gcgtaaattg gaaaatcatt ataaggatat gcaagacctg gagtttacca ttcaggaaga
     1201 taagctttgg ctattacaaa cacgtagtgg taaacgaaca gggatggcaa tggtaaagat
     1261 cgcaatggac ctgtatcgcg agaataaaat ttcaaaaaat gaagctttat taatgatcga
     1321 gccaaacagg cttaatgaac tgttgcatcc cgtatttgat aaagctgttt tacaaaaggc
     1381 aaatgtcatt gccaaaggat tgccggcatc gcctggcgct gctagcggac aaatagtttt
     1441 taatgccgaa gaggctcaaa tgtgggcaaa tcaaggtaag aaggtgattt tagttcgcat
     1501 tgagacttct ccggaagacc ttggcgggat gaacgttgcc gaaggtattc ttaccgcgcg
     1561 cggtgggatg acttcacacg ccgcagttgt tgcacgtgga atgggtaaat gctgtgtgtc
     1621 gggtgccgga agtcttaaga tcaattataa aaacagaaca gttaaaactg ataacgcaaa
     1681 cctgaaagag ggtgattgga tttcgctaaa tggaacgaca ggcgaaattt atgaaggaaa
     1741 aatcggcact attgatccgg agcttagcgg cgactttaat cgtttgatgg aaatagctga
     1801 cagtattgcg aaaatggaag ttcgtacaaa tgctgatacc ccaaaggatg cattaaaagc
     1861 acgaaatttc ggcgctaccg ggattgggtt gtgccgtacc gaacatatgt tttttgaagg
     1921 caatcgtata acggctatgc gcgaaatgat ccttgctgat gatgaagaag gtagaagaaa
     1981 agctttagac aaacttttgc caatccagcg tagcgatttt gaaggaatat ttgaagctat
     2041 gcacgatttg cctgtaacag ttaggctgct tgacccacct ctacacgagt ttgtgccaca
     2101 tgatgacaaa gagcaagagg agatggccaa ggtgatgaat attgatgtaa gtattgttaa
     2161 agctaaggtg gaggctctgc atgaaataaa tccaatgctt ggacatcgcg gttgccgctt
     2221 aggtaatacc tatcctgaaa tatcagaaat acagtcacgt gccattattg aagctgctct
     2281 taatcttaaa aagcgtggta tagttacaaa acctgagatt atgattccgc tcaccggtag
     2341 ccttagtgaa atgaaaatgc aggaagatat tgttagagag accgttgaga aagtgtttga
     2401 agaacgtaaa gatacaatcg aatatatggt gggtaccatg attgaggtgc ctcgtgcagc
     2461 ccttttggcc gatgagattg cacaatcggc agagtttttc tcattcggaa ctaacgactt
     2521 aacccagatg acatatggtt actcaaggga tgatgctggt aagttccttg gcgtttatct
     2581 tgataaagga atattaccaa atgatccttt tcaaattctt gatcaagaag gcgttggaca
     2641 attgatgcgg gttgctgtga aaagaggtcg tgacgcacgt aaaaacctaa agattggaat
     2701 ttgtggcgag catggtggcg aaccttcgtc agttgagttc tgtcatacat taggcttcga
     2761 ttgtgtaagc tgttccccat tccgtgttcc aattgcaaga ttatcagcag cacaagctgt
     2821 tataaacgag aagatggcca aaaaagccgg aaaagtaact aagaacaaga agaagaaaaa
     2881 gaccaagaag taatctaaat aattagacat tggttagatt gaaccccgat taagtatttg
     2941 attacactta atcggggtta ttttttaaca cacagcggtt ggattttgat aaagtatggc
     3001 ttatgtttta gtaaggcgct aaaaaattac aatagtcaga aagggttttt aaaggagttg
     3061 tttctggatg caggtaaaag gttttagaca cggtaataat ttttcaattc gacatcattg
     3121 ctattgacaa agtggaggtt gtatttatag aagttaaaac ctctcttcac catcaagata
     3181 ttaatacatg tcatgaaagt atatggaaag tgaagtagta aatgcctgaa tttgaaaaca
     3241 agccttttta tggcgctgtc gcttttctta ttgctaatag ctccagtgat tatatggctg
     3301 agaagatcgt ctaatttgta atcagcgctc ccagcgggag cgcctccatt atcaataata
     3361 aagtttttac atcaaaggcg ttttaatcta tctgttggtc taagaattac tcattggatg
     3421 atttaaagtt atccggcgag gttaacgggt tattgaatgt tttcactttc cggtaacccg
     3481 tattcttcaa tcattcttat atccaatgct tctaagctat ctaagatagg gttgtatatt
     3541 tcggggatta ctggtatgtg tactccgcgg gcattgattt taccttcgag tatcatctcc
     3601 acgcctacgg cagcaggtaa agctactgtg cgggctattg ctgtgtcggt tgctggggta
     3661 ccaaaatcga gcatcctcga tttgataacc tcttttgacc catcggggta tgatgccaga
     3721 aaagtgtgtt gcatcacaac catgtcgcgc tcgttatagc ctagcatcat tttactgatc
     3781 atcatatcgg aggtgatttc gtagggcgta tcttttcccc gtcccattgg tttgtcgtca
     3841 aatagtccga gccattccat agcatcaaag gcgtagctat ctgctggaag attaatatat
     3901 ttcgcagctt ttttccgaat gtcggtagga tctttttcgc caataagttt agctacaaag
     3961 ccggcatagg tcagattggt gaaatcatat tcgtcgctgg tgatcaggtg caggcgtttc
     4021 attgcgtcca atgtttcaca ccatctcggg aatcgaaagg tacctctgaa gattgttttt
     4081 gtttcgggga taccataaat attaatgtat gggattgagt cgcggtttgg ataaacttca
     4141 agttcgccga catcgggaaa gtcaacgatg aatggatttt taaatatgtc tatcggttca
     4201 atatatattt cttttcctct ttttaggaaa tggccatcgt tgttaccggc cattacaacc
     4261 ccttttgggc tccacgagaa tttatagtta aaggggttat cgcaggcctc cggtgcaggc
     4321 aaagctccac agagcgaata aaactcttct attttaccct ttttattgtg aacatagtca
     4381 atgatacgca ttgccgacat gtggtcaata cccggatcaa gtccaagctc attaagaatg
     4441 ataattccag ctttttttgc ttctgaatca agagcttgca taggtggttt tacataagat
     4501 gtggtaacca tgtttttctt atgtgtgata catattttgg ctactataac atgatatgcc
     4561 cagggtaaca ggctcactat caaatcgtga tcctttatca tgtcgtttag tcccggttca
     4621 tcatctactg tccatgcgat agattttccg tttttgtgtc cgccgatcat gttatcggcc
     4681 tttgattttg tgcgggttgc aacggtaaca tagtaaccgc gttcgagcag gtaggctact
     4741 attggcttca ccacaagacc tgcacccaaa atgagtactc ttttcatcga aattgattta
     4801 taaattgtta aaaaatatta atgtcaatcg agatatttct gaagatactg gtaatcaggg
     4861 gttagtttcc ctttatggag aatgagcgct cgttttatct ctggtggaag ctctaatttt
     4921 tcgaaactta cattgtaatt agctcttact atttcgggga tgaaatcaat aagcgcgtcg
     4981 ccaaatgctt ctgaggagtc gcgcggtagt tcgctgggca ggatatcaac agccatcaca
     5041 aggataccat ttcctttaaa gcccattgta ggggtacgat taaccggatc ataaacaaat
     5101 acggggtctt cgatagcggt acctttatga gtgaattcaa tcgagccatc gggatcgcac
     5161 gtaacatcgc ctatcacttt gagcttcata tcacctttct tatatagctg ttctatatcg
     5221 tcttttgaga ctaaacgtgg aaaattctca tcccaataca tacagttcat taggatggtg
     5281 aggtgaggaa ggtatttttg gaacgagttt ttatagtttt cgggatgttg atgaaattct
     5341 tttaggctaa aaggttttcc tggctcaatg ggttcggtaa tatcatcttt ataaaaaacg
     5401 gttttgtata tcagcttgtt tgaataattt cccgaagctt tgagattaat taattcggca
     5461 ggtgtgattt ctacagcagg taataagttg gctatatgtt gcgagccttt actaacgttt
     5521 cctctacctg tgaaccctat ggtaaatggc gccatttcgt cgggtaatcc ttgagcgcgt
     5581 attagttcac caacttcacg tatggcctgt tcggcatcgg caagagaatg gtagtttact
     5641 gactgtttga tcctgaggaa tggcgtttca acaccaagca ctttcatcct tgcaccaaac
     5701 gaccacaggg aattgatcat accggccaaa ccagcaaagc ggccaaaaaa tatcaaacgc
     5761 ctccccgaat cgtcaacaat gcgttcgtaa tcaataagat tacaacgttt gcccatcatt
     5821 tcggaaagca tcggcatatt gtaaggctgc cccttgataa catgtgaaaa aaagatgtag
     5881 gtcttctcct cttcgaacca cgatgcagga atctctttta ctccaaatat cacaggtatt
     5941 tctgagaggt tgtcagttat tactgctcct gcatgcttgt attcgttttc gtgaaatact
     6001 ctttttttag atcgctccac atgtacttct ataccgtgcc cgactaactt tttaacatgt
     6061 cggggcgtta aggcgctacg tcgctccatc aggtatttgt cttcgtgcct gataccaact
     6121 tttctgttca taatcatctt tttaaaaaaa aaattacgcc acaaaattac ataaagttgt
     6181 taggtcgaat tgtggtagga ggtaaattat aaatgtttta aatagatcga aagaaaaagg
     6241 agaatgggtg agtggaggag aagcaattta acctgataaa tgcttttact ggatgtgaag
     6301 aatggaggct ttaaaaaaaa attcactatt taatttccta ataattaata tgttattaac
     6361 tatgttcagt aaatacccaa gcgaaaaata gcgattattg ctttttgttt aataattatt
     6421 ttatttttgc cggctgaatt aaaaaagttg ataatttaat taaatttaag atggattatg
     6481 agaattgtgt ttacgaaaca caaaaaaatc tttattacaa aaagatttaa ataattaatt
     6541 cccacttttt tttaatcttt ttcatttaaa atcagttaaa tgtatccatg tgtaaggata
     6601 tgtaaatatt agtttgttta attgagtagg atgattaaac ataaaaaata cctttgctga
     6661 tttttagaaa aataatttgt tattagcttt tcccttttag ttaaaggact tttacatgga
     6721 atgtttggca aattaatatg agttttaaat atttgtttag tttaaaaaat tctactatcg
     6781 tgtgtttcaa aagaattact ccttcaatac catttagtat gtcctgtacg gctctttttg
     6841 gatttcaatt taaacgtaat cattcatcat attggctttc tgccgtattg atttcgtttt
     6901 ttctaatgat aaccgctatt ggatttgcgc aggcgcctgc gccggtgtgg cacacttcag
     6961 ggctaaaaac caattttggg atcggtacag ccgtgtctgt taacagacct gatgctgttg
     7021 tggctggtga tttgatcctt ttgttttttg cacagacaaa aagctcaatg attgattcca
     7081 tcagtggatt tactactcct accggtttta ccatgattca ttccgaacat aataccacta
     7141 ctaatttacg accggaggta gtggcttttt ataaaattgc cggtggaagt gaacctgctg
     7201 cctataccag tacagcgaca gattttcttt ctacagcgcc aatgtggaaa gcagtggcag
     7261 tgagagtgac cggacacgat cctactaatc caatacaagg acatagtgga aatagcccga
     7321 ctttagccac tacatcggtt acacttcctt ctttaacaac ggttgtttcc aatacactat
     7381 tagtaagcgc cgttactgtt cgccggtcta tttcctcaat caatgtaaac cctatgacgc
     7441 tgcagtggtt tttggatggt acaggaacca ccgatgatag ctcaaatgaa ccttcacttt
     7501 tcggatctac acaagttatt ccttctgccg gttctaccgg tacgcgtcag cttacatgga
     7561 gcggctcagg acgtgccgcc ggattgatgt ttaccattaa tccatcttta gttaatgctg
     7621 atttggaagt tacaatgagc gtaaatccgt ccagctcgca ttattttgcc gatactgtta
     7681 actataccat tactgcaacc aacctgagta attcacttaa tggcgctacc ggtgtggtag
     7741 ttactgatct cttacctcca ggcttaggat taacatctca taccgcttct accggcacaa
     7801 cttatactcc tgcaactggt aagtggaata ttggaaacct tgctaaaaat gagagtaaga
     7861 ctcttacgct gcaagtaacg attgtatgtt ttgagaccaa ttttaagaat gttgctacta
     7921 ttatcgggaa cgaattcgac ccgaatatct taaacaacca ggccgaaata acactaagtc
     7981 ccatgaccgg cgggtcaaat ccatgcccca taattgccaa tgatgatatt gcaaatgggt
     8041 tacaaggaga acctattatt atccatgtaa ctaataatga taaaggagaa tttgacgttt
     8101 caacactctc tattaccgta caacccatta atggtacaac tcagatagta caaaatggaa
     8161 taatccacta tcttccaaat ggtaacttta ctggcaccga ccaatttaca tatagaattt
     8221 gtaatacggc agaaactatt tgtgatgttg ctaccgtata tgtaaccatt accgaagact
     8281 acaccagccc ctgtcttgaa gctacgcgcg ataaactctt ttatcttcct tttccggaaa
     8341 acaaagacca gatgagagag gcattgttat cagctggtag ctattacat
//
LOCUS       DAOOAI010000093         8356 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_10395_length_8356_cov_5.173353, whole genome shotgun sequence.
ACCESSION   DAOOAI010000093 DAOOAI010000000
VERSION     DAOOAI010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8356)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8356)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8356
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10395_length_8356_cov_5.173353"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(366..1880)
                     /locus_tag="PK345_07105"
     CDS             complement(366..1880)
                     /locus_tag="PK345_07105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HPX34819.1"
                     /translation="MTISIELILLVGSALFFISMLVGQVGHRFGVPILLLFLLVGMVF
                     GADGFGLEFENIQMAQAIGSVCLAIILFSGGLDTRISEIKPVIGPGVVLATLGVLMTA
                     IFTGLFTYWMTDNFFPALGMGLLSCMLLASTVSSTDSASVFAILRSKGLTLKNNLRPL
                     LELESGSNDPMAYLLTVMFIDIIQYGDNPNYLVVGLNVVIQLVVGTGMGILLGRLAVK
                     IFNKVKMDNDSFYPIMLLVFGVFIFASTYFLKGNGYLAVYIAGLTIGNSRFVHKRSSM
                     RFMNGFAWMSQILLFLTLGLLVNPSELVDVIVPALIISFFMIVFARPITVFLCLIPFR
                     KVGFKDKLYISWVGLRGAVPIIFAILPLAAGVPGARLIFNIVFVLTLVSLLVQGTTLP
                     AVARLLGLAEKPKQYRPFQDFDVDFSEEIKSVMTEILITDETLKYGKNLMEIQLPDKT
                     LAVMVKRGDNYFVPTGRTKLHPNDILLVISDDEKALKEAYKNIGISDFTYRKNQ"
     gene            complement(2201..2974)
                     /locus_tag="PK345_07110"
     CDS             complement(2201..2974)
                     /locus_tag="PK345_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34820.1"
                     /translation="MKKPCLIFLALSLVASIFCLGCEDTYLGSEDTNIEREDTDLNSD
                     PDFEFLKGTWIDKGSLAMRFIDFYSEKEARIGIYGKNFKKFEPFNYRIINSNQIAVSW
                     IDDKQPNVSFHDLIKIGNDTIEISDLTDLPENPNKIYLRREILTGKNNDTIVIGHKQE
                     YFDFENDLRLRIDPILNDSRCPTGVECIWRGNAEVRIELIIQGAFWHYFILNTNCAYR
                     TDTVIDNIRYRLVALEPYPVINQPIDFRDYVAKILIEKQ"
     gene            complement(3146..3661)
                     /gene="cobO"
                     /locus_tag="PK345_07115"
     CDS             complement(3146..3661)
                     /gene="cobO"
                     /locus_tag="PK345_07115"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012573353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HPX34821.1"
                     /translation="MRGYIHIYTGNGKGKTTAALGLSLRAVGAGKKVFFAQFVKGEIY
                     SEVKAIQKFLPDITIRQYGLKCFIYEKPSQADIDRARKGLKEVSEVILSGKYDVIVLD
                     EANIAVYYNLFTDEELINIIRQKPEQTEIIITGRYASDKLIEFADLVTEMKEIKHYYN
                     QGVQARKGIES"
     gene            complement(3875..5350)
                     /locus_tag="PK345_07120"
     CDS             complement(3875..5350)
                     /locus_tag="PK345_07120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019540480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="von Willebrand factor type A domain-containing
                     protein"
                     /protein_id="HPX34822.1"
                     /translation="MNTMKTKLKISVCCLLIAFLGSCETQDNGIYMEYITGMQTLDND
                     IYGEKYKDYEENPFIKTADQPISTFSVDADGGSYANMRRFLHLGQTPPKESVRIEEFI
                     NYFTFDYKEPVKDENVSLESEISNCPWNTEHHLLRLGMKGLTIPAYELPNSNYVFLID
                     VSGSMNSPDKLGVLKTGFKTLVDNLSDRDRIAIVTYAGQAGVLLESTYGDEREKIKSA
                     IDKLGAGGSTAGAAGIITAYEIAEKNFIPNGNNRVILGSDGDFNVGPSSTEELIKLIE
                     EKRDKGIYLTVLGVGGGNLNDYMMEQIANKGNGNYEYIDNAKQIEKVFTHEILKFYTV
                     AKDSKIQITFNPNKIDSYRLIGYENRKLEKEDFENDSTDAGEIGSAQTITALYELVLI
                     DKPDEEKYAQFDFRYKKPNETESRLITHEINSVPKEIMSSSENMRFATSIAGLGLLMK
                     QSQYKGTLTKQMVLDLGRGAISFDPNGYRKEFIDLVTNWKN"
     gene            complement(5529..6653)
                     /locus_tag="PK345_07125"
     CDS             complement(5529..6653)
                     /locus_tag="PK345_07125"
                     /inference="COORDINATES: protein motif:HMM:NF016497.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HPX34823.1"
                     /translation="MKKGIILLFIALFGILMTGLAQQPGDDKTVEVKTREFRYTNPIT
                     RDTAISMRDHCIIKVGDKWYCTGTSNPVWTGPNPGVRLLVSDDLIHWEQHSWLIDAGK
                     LPEDCPYNGRFWAPEIHYIQGKYWLTVNSGKVTKEDPKGMDTHSVWLFSSDEVTGPYS
                     LVNGPLTPQYNNDATLFEDEDGQVYFYCSGNGLFQAKIDLKTGKLTTPIEKFLDKRQP
                     GWPEWMVGGIEGPFVIKRDGTYFMFFSTWTRGYEVGLLKSKTPLGPWELVSEEPIFVT
                     RKKGYRPELAIGGGYDHLKFQDTEDPYCETGHNALFIGPDGNIWSSCHYMGYEQRPYP
                     YCQELQDWEKMPQMGYEPVYYENGRFYINGPTWTEQIIEY"
     gene            complement(6734..7411)
                     /locus_tag="PK345_07130"
     CDS             complement(6734..7411)
                     /locus_tag="PK345_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34824.1"
                     /translation="MKKQTDLTILCRMRQNFSLRRWFLVLIVIMASTQLYAGFFDSLS
                     IGIGGGYDSYNTSRVELYLKSDLKVFNRKAEIKAGLNNRSYQLEFDGVKDLDAQSIGF
                     FGDFAVYPFNTGLFTGLRWESINFNWLTPESMSKVRRERNYSATSLYTGTCIFFPIGY
                     NFQLSDKIALKLYAQPGFQQFGISNGSYSSGSYVFVNGSSSGNMVYESHYEFIYNINI
                     SLEFKIK"
     gene            complement(7743..>8356)
                     /locus_tag="PK345_07135"
     CDS             complement(7743..>8356)
                     /locus_tag="PK345_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34825.1"
                     /translation="DGIERRTGYSSGYTSGARVLKFTGSSRGLNNGLYFCNASGNIRQ
                     GWAKFGLPDGGTTLSLSPGQYTLRYRVCNWDRPDFSPVELRIERRTDGVRIASQTYTP
                     TVNIGNDVSNNFSAPEQQYFEFEVTEPGDYVIAFYAASAQSADGVLGQLSLAANSYIP
                     TGIKEVYDLQLSEDVIYDLKGQRVDTPTETGIYIRNGRVIFIK"
BASE COUNT         2485 a         1839 c         1424 g         2608 t
ORIGIN      
        1 tctttaatcc tggtttccat aatttaatct ctgttgctgt cagtataagt tatattataa
       61 tttgatccgg taatatactt atatttatct cttgtgtcgt aaacgtcaac ctactttgct
      121 tattacttta tcttacgcgt atttaagttg ttttatatgt ttttaatcaa tccattagat
      181 tttaaaatca ttgcagcaga aataacgcaa atatataaat tattaaaacg gagtgcttat
      241 gacatacccc ggaaagttag ataaaaactt ttgaggatca tgtcacttcg accccgtttt
      301 agcaatctgt tattaaatat caataggaaa agatgcacat attctgctct tttcgccctt
      361 caagtttact ggtttttccg atacgtgaaa tctgaaatac cgatgttttt ataagcttct
      421 ttcaacgctt tttcatcgtc cgatattacc agcaatatat cgttgggatg aagtttggtc
      481 cgtcctgtag gaacaaaata attatcgccc cgctttacca taacagccaa cgtcttatcc
      541 ggcaattgta tttccatcag atttttacca tacttcagcg tctcatcggt aatcaatatc
      601 tccgtcatta ccgattttat ttcttccgaa aaatccacgt caaaatcctg gaaaggtcta
      661 tactgtttag gcttttcagc cagtcccagt aagcgtgcca cagccggcaa tgtagttccc
      721 tgtaccagca gcgaaaccag agtaagtaca aacacgatgt taaatatcag ccgtgcaccg
      781 gggactccgg ctgccagagg caaaatggca aaaatgatag ggaccgcgcc acgtaatccc
      841 acccacgata tatacagttt gtctttaaaa cccacctttc ggaaagggat cagacacaag
      901 aaaactgtaa taggacgggc aaatacaatc ataaagaaag agataatgag cgcaggaaca
      961 attacatcaa ccaattcact gggattaacc aacaaaccga gcgtaaggaa cagcaatatc
     1021 tgactcatcc atgcgaatcc attcataaat ctcatggaag agcgtttgtg tacaaatcgg
     1081 gagtttccga tagttaaacc tgcaatataa actgccagat aaccgtttcc ctttaaaaag
     1141 taggtcgagg caaatataaa caccccgaaa acaagaagca taatcggata aaatgaatca
     1201 ttatccattt taaccttatt gaaaattttt accgccaaac gccctaacag tatacccatt
     1261 ccggtaccca caaccaactg gataaccaca ttcagtccta ccaccagata gttgggattg
     1321 tcgccatatt gaataatatc gataaacata accgtcaaca gatatgccat agggtcgtta
     1381 ctgccacttt ccagttcaag tagcggacgc aagttgtttt tcagcgtcaa gcctttggaa
     1441 cgcaaaatgg cgaaaacgga agccgagtcg gttgatgaaa ccgttgaagc aagcagcata
     1501 cacgacagaa gccccattcc cagcgcaggg aagaaattat cagtcatcca atatgtaaac
     1561 aaccccgtaa aaatagccgt catcaacact cccaaagtag ccagtaccac tccggggccg
     1621 ataacaggtt ttatttccga aattcgggtg tccaaacctc ccgaaaacaa aataatggcc
     1681 aagcaaaccg agcctatggc ttgcgccatt tgtatatttt caaattcgag cccaaaccca
     1741 tcggcaccga ataccatacc caccaaaaga aaaagcaaca aaataggcac gccaaaacgg
     1801 tgccccacct gccctaccag catactaata aaaaatagag ctgaaccaac aagtaaaatc
     1861 agttctattg aaattgtcat ctataactgt tttaaaataa ttgaacttat gatgatttta
     1921 taaatataaa agttgttcac ataggattga atccacctac ctgcctgata atcaatcatt
     1981 taaatatatg caaaactttg ttgcaaagta cgagaaattt ctcgacatat taaaataaat
     2041 agatttaaaa aatgtgactt ttaaaacaaa tgttttccac gtctaattaa gtcaatggga
     2101 gacaactctt aatccgataa tagaataatc aaagttgttt taaaaaatat cctaagaatt
     2161 ttgatacttg catacactat ccgtttatcg gtagccgata ttattgtttt tcaattaaaa
     2221 ttttagcaac ataatcccta aaatcaatcg gttggttaat tacaggatat ggttccaatg
     2281 ctactaatct gtaccttata ttatcaataa cagtatccgt acgatacgca caatttgtgt
     2341 tcaaaataaa ataatgccaa aaagctcctt gtattatcaa ctcaattcgg acttcggcat
     2401 tacctctcca tatacattct accccggttg gacatcttga gtcattcagt attggatcga
     2461 ttcgtaacct aaggtcgttt tcaaaatcaa aatattcctg cttatgtcca attacaatcg
     2521 tatcattatt ctttccggtg agaatttccc gccttaaata aattttgttg ggattctcag
     2581 gaagatcagt caaatcactg atttcaattg tatcatttcc gattttaatc aaatcgtgaa
     2641 agctcacatt cggttgcttg tcatcaatcc aactgactgc gatttgattt gaattaatga
     2701 tccgataatt aaatggttcg aattttttaa aatttttgcc ataaattcca attcttgcct
     2761 ctttttcgga ataaaaatca atgaatcgca ttgccaagct tcccttatct atccatgtcc
     2821 ctttcaagaa ttcaaaatca ggatctgaat tcaggtcagt gtcctcacgt tcgatattag
     2881 tatcttcact cccaagataa gtatcctcac atccgagaca aaaaatggaa gccactaagc
     2941 ttaatgctaa aaatatcaaa caaggttttt tcatatctgt tgcttttttg agtcgatttg
     3001 aaatgcaatt taaaggtatg aataaacttc aggagataaa attagtaaat tattaaattc
     3061 acaacaatat taagccatga atttttataa atcgactgtt tcctatctat aatgctttgc
     3121 tttatatatc cgcctattct aatctctaac tctctatgcc tttccttgcc tgaacaccct
     3181 gattatagta gtgttttatt tctttcatct cggtaactaa atcggcaaat tcaattagtt
     3241 tatcggaagc ataacgacct gtaataataa tttcggtctg ttcgggcttt tgcctaatga
     3301 tatttataag ctcctcgtca gtaaaaagat tatagtaaac agctatgttt gcctcatcta
     3361 atacaatcac atcatatttg cctgataaaa tcacttcaga tacctcttta agccccttgc
     3421 gggctctatc tatatcggct tgcgagggtt tttcatagat aaaacatttt aacccatact
     3481 gccttatggt aatgtcaggc aagaattttt gtatcgcttt tacttccgaa taaatttccc
     3541 cctttacaaa ttgcgcaaaa aaaacttttt tccccgcacc aacagctctt agtgataatc
     3601 ctaatgctgc ggttgttttt cccttgccgt tgcctgtata aatatgtata tatcccctca
     3661 tttataagaa attcaagcca aacaaatgta aagatatgtt ttttgcaaag aatatgcagg
     3721 ttattttgca caaaaaatgc ggagaataag tatgatattc tccgcattac tttaaaaccg
     3781 ctcagtgatt ccgtttacaa ccactcagtg aatttgttta taacctttct gtggcttgaa
     3841 ctgtacccga aaccgagttt attgcaatta attgctagtt tttccagttt gtcactaagt
     3901 ctataaactc ttttctgtaa ccgttcggat caaaagatat tgcacctctt cctaaatcca
     3961 acaccatctg tttggtgagt gtccctttat attgagactg tttcatcaga agtcccaatc
     4021 ctgcgatcga tgtggcaaat ctcatatttt cagatgaaga cattatctcc tttggaaccg
     4081 aattaatctc atgtgtaatt aatctgcttt cagtttcatt cggttttttg tatctgaaat
     4141 caaattgagc atatttttcc tcatcaggct tatctatcaa tacaagctca tacaatgctg
     4201 ttatcgtctg tgccgaacca atctcgcctg catcggttga gtcgttttca aaatcctctt
     4261 tttctaattt tcggttttcg tatccgatta acctgtatga gtcaatcttg ttcggattga
     4321 atgtgatctg tatcttcgaa tctttggcta cagtgtagaa tttgagaatc tcatgagtga
     4381 aaaccttctc aatctgtttg gcattatcga tatactcata atttccattg cctttgtttg
     4441 ctatctgctc catcatgtag tcattcaagt ttcctcctcc aactccaaga accgtcagat
     4501 atataccctt atcgcgtttt tcctcaatta atttgatcaa ttcttcagtc gatgaaggtc
     4561 ctacattaaa atcgccgtca ctgcccaaaa tcactctgtt gttgccgtta ggaataaaat
     4621 tcttttcagc tatttcgtat gctgttataa tgccggcagc tcctgctgta gaacctcctg
     4681 cacccaattt atcgattgcc gattttatct tctccctctc atcgccataa gttgattcca
     4741 acaaaacacc tgcctggccg gcatacgtga caattgcaat tctgtcccgg tcacttaaat
     4801 tatcaaccaa tgttttgaag ccggttttta atacacctaa tttatcggga gaattcattg
     4861 acccggaaac gtcaattaaa aacacataat tggagttagg caactcatag gccggtatgg
     4921 tcaatccttt cataccaagc cttagcaggt gatgctccgt attccagggg cagttcgaaa
     4981 tttcagattc caaagagaca ttttcatcct taacaggctc cttataatcg aaggtgaaat
     5041 aattgataaa ctcttctatt ctgactgact ctttgggtgg agtctgtcct aaatgaagga
     5101 acctcctcat attggcatat gaaccgccat cggcatccac agaaaatgtg gaaatcggct
     5161 gatctgccgt tttgataaac ggattctcct cataatcctt atatttttca ccatagatat
     5221 cattatctaa tgtttgcata ccggtaatgt actccatgta aataccgttg tcttgcgttt
     5281 cacaacttcc taaaaatgct atcagcaagc aacaaaccga gattttcaat tttgttttca
     5341 ttgtgttcat tgtatattat ttattagact gtctaagtta taaaaaatat cctttttatt
     5401 ttacatctgt ttgcgtcagt atttcattaa aaaaatccat ttccactcaa tacatgccgg
     5461 tcttgatgcg gagtacaaat ttacaagttt tgatataata tctcactaaa aactgttaat
     5521 attaattact aatattcaat aatctgttct gtccaggtcg ggccgtttat gtagaatctg
     5581 ccgttttcgt agtaaacggg ttcataaccc atttgaggca tcttttccca atcctgaagt
     5641 tcctgacaat atggatatgg gcgttgctcg tatcccatgt agtgacatga gctccagata
     5701 ttaccgtcgg ggccgatgaa caaagcatta tggccggttt cacaataggg atcttcggta
     5761 tcctgaaatt tcagatggtc atatcctcct ccgatagcca attcggggcg atatcctttc
     5821 tttcttgtta cgaaaatagg ttcctcggaa acaagctccc atgggccgag cggtgttttt
     5881 gatttcaaca gcccaacttc atatccgcgt gtccacgtag aaaagaacat aaaatatgtt
     5941 ccatccctct ttataacaaa cggaccttcg atgccgccca ccatccattc aggccagccg
     6001 ggttgtctct tatccagaaa tttttctata ggagtagtca gttttcccgt tttcaaatca
     6061 attttggcct gaaacagacc attaccgctg caataaaaat agacctgacc atcctcgtct
     6121 tcaaaaaggg tcgcgtcgtt attatactga ggagtaagcg gtccgttaac caaagagtat
     6181 gggccggtaa cttcatcaga tgaaaagagc catacgctat gggtgtccat cccttttggg
     6241 tcctccttgg tgacctttcc actatttacc gtaagccagt atttaccctg aatgtaatga
     6301 atttcgggag cccagaacct gccgttatat ggacaatcct caggcagttt gccggcatca
     6361 ataagccatg aatgctgttc ccagtgaatg agatcatcgg aaaccagcaa ccgaactcca
     6421 ggattcgggc cggtccacac cgggtttgaa gttcctgtac aataccattt gtctccaact
     6481 tttatgatac agtggtcacg cattgagata gcagtatctc tcgttatggg atttgtatat
     6541 ctgaactcgc gggttttaac ttccacagtt ttatcatctc cgggctgctg ggccaaaccg
     6601 gtcattaata ttccaaaaag agcaataaac aacagtataa ttcctttttt catctcaaat
     6661 attttttaaa cagttcgtta ctttctatta taaggactct cctgcatctt taaaaacata
     6721 tcaaagcacg tttttatttt atcttgaatt cgagactaat attgatgttg tagataaact
     6781 cataatggct ctcataaacc atattgccgg atgaagaccc gtttacaaaa acataacttc
     6841 cggaggaata tgacccattt gatataccaa attgttgaaa tcccggttgg gcgtacaatt
     6901 taagagctat tttatcggaa agttggaaat tatatccgat tgggaaaaag atacaagttc
     6961 ccgtataaag agatgtcgcc gaataattcc tttctcttct tacctttgac atcgattcgg
     7021 gagtcagcca attgaaatta attgactccc atcttaggcc ggtaaataat cccgtgttaa
     7081 acgggtaaac tgcaaaatcg ccaaagaatc cgattgattg agcatccagg tctttaacac
     7141 catcaaattc caattgatag gagcgattgt ttaatcctgc ctttatctct gcctttctat
     7201 taaatacttt gaggtccgat tttaaataga gctctacccg tgaagtattg tagctgtcat
     7261 atccgccgcc tatacctatt gaaagcgaat caaaaaaacc ggcatacaat tgcgtacttg
     7321 ccataatcac gattaaaacc aagaaccacc gtctcaagct gaagttttgc ctcatccgac
     7381 aaagtattgt caaatccgtc tgttttttca tgcctttatc aattaaacta tgtttagtcg
     7441 gatacaaatg tatttaaact tttatagtta tgaaatgttt gattgaaaat ttttaatgta
     7501 ctccggctcc ttcccttaaa atgcacaaca acggcatgca tctattttat cgactcccca
     7561 ttaataccgc attttacaga ttcagatgcg ggtacagatg tggaagcgaa ttcggaaacg
     7621 gaaacggaag cggacccgga ttcgatggct gaaccgatgt gttttcaaca ttatcgccta
     7681 aagtgtgata atcaagacgg tattccagat atgcaaaaat aaatcctttg atccctgcgt
     7741 ttttatttta taaatattac tctaccgttc ctaatataaa tacccgtttc ggttggagtg
     7801 tccacacgtt ggcccttaag gtcatagatg acatcctcac ttaattgtaa atcgtaaacc
     7861 tctttgatac cggtgggtat atagctgttg gccgccagac tgagctgccc taaaacaccg
     7921 tcggcagatt gtgcactggc ggcgtagaag gctatgacat aatcgcccgg ttcggtaact
     7981 tcaaactcaa aatactgctg ctcgggggcg ctgaagttat tggacacatc gttgccgatg
     8041 ttgacggtcg gcgtataggt ctgactggct attctcacgc catccgtgcg tctttcaatc
     8101 ctcaattcca cgggcgaaaa atcgggcctg tcccagttgc atactctgta tctcagcgta
     8161 tattgaccgg gagacaggct gagcgtcgta ccgccgtcag gaagtccaaa cttggcccag
     8221 ccctgacgga tgtttccact ggcattgcag aagtagagtc cattattcag tccccgggat
     8281 gaacccgtaa actttaatac acgagcaccc gacgtgtagc cgctgctata tcccgtgcgc
     8341 cgttctatgc cgtcgt
//
LOCUS       DAOOAI010000094         8222 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_10575_length_8222_cov_5.864087, whole genome shotgun sequence.
ACCESSION   DAOOAI010000094 DAOOAI010000000
VERSION     DAOOAI010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8222)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8222
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10575_length_8222_cov_5.864087"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            38..490
                     /gene="rpiB"
                     /locus_tag="PK345_07140"
     CDS             38..490
                     /gene="rpiB"
                     /locus_tag="PK345_07140"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009317756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HPX34826.1"
                     /translation="MTQEQQLIPIGSDHAGFKLKEFLIESLITKGFSFEDFGTFYEES
                     VDYPDFIHPVAAWINKHPDKKGIVICGSGQGANMTANKYLKVRAALCWNVEQAKLSRL
                     HNDANIITLPGRFIDFEEAVECVKVFFLTPFEGGRHIKRVEKISAIIH"
     gene            502..1893
                     /locus_tag="PK345_07145"
     CDS             502..1893
                     /locus_tag="PK345_07145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HPX34827.1"
                     /translation="MSEYYKKFLKDSEQVAFDTFHREKINNTIEKYNIAVRSGKRQYE
                     NLDLAKNRAAGIKHKVLENLDKYLYEFELNFENNGGRIIWAPSASDALKAIVAILKRN
                     KAKLVVKSKSMITEELELNKVLENESIETLETDLGEFIVQVANEKPYHIVTPAMHKSK
                     EEVAELFSEKFGIDKSSTPEQISAFVREKLRDKFINADVGITGVNFLIAETGSLVLTE
                     NEGNGVLTSSIPKIHIAISGIEKIIPTLRDLDLFLPLLAVHGTGQNITAYNSIISGPK
                     QNDETDGPEELYLVLLDNRRTEILKHKELRRSLSCIRCGACLNACPVYKNVGGYTYNS
                     TYTGPIGAIITPHLRGMNEFGHLSFASTLCGKCTEVCPVNINLHELLLINRQELVKFG
                     IVSKKERQAQKWTYRVLKKRKRMEIANATLKNRAINLLFKKTWGPRRTLPVFAEKSFN
                     KIWEEKNKEKNKS"
     gene            complement(1927..3225)
                     /gene="tyrS"
                     /locus_tag="PK345_07150"
     CDS             complement(1927..3225)
                     /gene="tyrS"
                     /locus_tag="PK345_07150"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012457449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HPX34828.1"
                     /translation="MNFIEELQWRGMIHDMTPGLTEQLENEMTTAYVGIDPTADSLHI
                     GHLVSVMMLKHFQVCGHRPIVLIGGATGMIGDPSGKSKERNLLDEQTLRQNQESIKNQ
                     LSKFLDFNQNKPNGALMVNNYDWMKEYSFLQFIRDIGKHITVNYMMAKDSVKTRLSAE
                     SKEGMSFTEFSYQLVQGTDFLVLFQQHNCKLQMGGSDQWGNIITGTELIRRKLGHDAK
                     AFALTCPLITKADGGKFGKTEEGNIWLDPAKTSPYSFYQFWLNTSDEDAISYIKIFTL
                     LRKEEIDQLIENHRKAPHQRELQKVLAREITIMIHSKEEYELAVDASEILFGKGTTDT
                     LKRLKEDVLLNIFEGVPQSNVSSNDIYKSIPIVDFLTDFTKISSSKREAREFLRNGAI
                     SINKERVGEDYMIGENDLLQNRYILAQRGKKNYYLIICDY"
     gene            3383..4462
                     /gene="fbaA"
                     /locus_tag="PK345_07155"
     CDS             3383..4462
                     /gene="fbaA"
                     /locus_tag="PK345_07155"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HPX34829.1"
                     /translation="MAEKIFDFVKPGVLTGNDVQKVFAVAKKNQFALPAVNVVGTDSI
                     NAVLEAARKVNSPVIIQFSNGGASFFAGKGLSNENEKAAIVGAISGALHVHRVAEMYG
                     VPVILHTDHAAKKLLPWIDGLLDFGENFFETHGKPLYSSHMLDLSEEPLKENVEICKK
                     YLERMKKIGMTLEIELGITGGEEDGVDNTDVDSSRLYTQPEEVAYAYEELIKVSNRFT
                     IAAAFGNVHGVYKPGNVKLRPIILKNSQDYIQKKFNTEVNPINFVFHGGSGSSREEIR
                     EAISYGVIKMNIDTDTQWATWEGVMNFYKKYQGYLQGQIGNPEGEDKPNKKYYDPRKW
                     LREGQSSMVKRLELAFEDLNNINRN"
     gene            4594..5721
                     /locus_tag="PK345_07160"
     CDS             4594..5721
                     /locus_tag="PK345_07160"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HPX34830.1"
                     /translation="MKEIRMVDLKGQYERIKSEIDTAIKEVIESTAFINGPVVKEFQN
                     DLENYLGVKHVIPCGNGTDALQVAMMGLELKPGDEIITTTFTFIATAEVIALLKLTPV
                     LVDVDPDTFNINPDEIRKAITHKTRAIVPVHLFGQCADMDEIMKIAKEHGLYVIEDAC
                     QAIGSEFIFENGERKKAGTIGDAAGVSFFPSKNLGCFGDGGAIFTNNDNFAKLMRSIV
                     NHGMTIRYHHENIGVNSRLDSIQAAILNVKLKYLDKYIKARQEAANYYDKKFAGHQKL
                     KIPFRVKNSTHVFHQYTLVVDDVDRDELQKFLASKNIPAMVYYPIPLHLQQAYKDPRY
                     KEGNFPVSEYLSKHVISLPMHTELDEEQLGYITNTLLEFIK"
     gene            5728..6309
                     /locus_tag="PK345_07165"
     CDS             5728..6309
                     /locus_tag="PK345_07165"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013611252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HPX34831.1"
                     /translation="MEKSYFAHETAVIDDGCTIGNGVKIWHFSHIMKDCVIGENSNIG
                     QNVVVSPHVILGKNVKVQNNVSIYTGVICEDDVFLGPSMVFTNISNPRSAVVRRDKYE
                     KTVVRQGATIGANSTIVCGNNIGEYAFIGAGAVVTKEVLPYALVVGNPARQIGWMSEY
                     GHRLHFDENGYATCPESNERYELYEGKVRKVKE"
     gene            6352..7641
                     /locus_tag="PK345_07170"
     CDS             6352..7641
                     /locus_tag="PK345_07170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008771971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HPX34832.1"
                     /translation="MYQRLIKKEAKISVIGLGYVGLPVALEFAKRVPVIGFDIKSDRI
                     EMMKNGIDPSNELSKEAFDGCDIFFTDNPKDLQNADFHIIAVPTPIDDSNIPDLSPLL
                     SATKTVAGILKKGDYVVYESTVYPGATEEEAIPLLEEISGLKYKRDFKVGYSPERINP
                     GDKLHTLATVVKVVSGCDDESLETIAKIYEMVVTVGVHKAPSIKVAEAAKIIENTQRD
                     VNIALMNELSIIFNRMGINTYDVLEAAGTKWNFLNFYPGLVGGHCIGVDPYYLAYKAK
                     KYRYHPQVINSGRFVNDSMGFYVAKQAVKKMINAKKNISDCKVLVMGLTFKEDVSDIR
                     NSRVPDIITELKSYAVNVDVMDPLADNEEVKKEYGFELISSITNKYDAVIVAVNHLKY
                     KNLNEDFFKSITTQNAILIDVKGIYRNKIKHLNYWSL"
     gene            7756..>8222
                     /locus_tag="PK345_07175"
     CDS             7756..>8222
                     /locus_tag="PK345_07175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008622763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPX34833.1"
                     /translation="MKKVLVTGGTGYIGSHTVVELQQVGYDVIIVDNLSNSQPDVLEN
                     ITNITGIKIDFEKFDLADRKKTLDFFKRHKNIDAIIHFAALKAVGESVVQPLNYYYNN
                     LFSLMNILQGMLDNNIENLVFSSSCTVYGQPANLPVDESAPVQIALSPYGNTK"
BASE COUNT         2773 a         1252 c         1618 g         2579 t
ORIGIN      
        1 ttttattttt gaaaaaaaat tttcaacaaa actataaatg acacaagaac aacaacttat
       61 accaatcggt agtgatcatg caggttttaa gctgaaggaa tttttgatag aatctttaat
      121 aactaagggg tttagttttg aagattttgg aacattttac gaagagagtg ttgattatcc
      181 tgattttata catcctgtag cagcatggat taacaaacat ccggataaaa aaggaattgt
      241 tatctgtggt agcgggcaag gagccaatat gactgcaaac aaatatttga aagttagagc
      301 tgcgttgtgt tggaatgtcg aacaagcaaa gctttcgagg ttacacaatg acgcaaatat
      361 aataacttta ccaggacggt ttattgattt tgaagaggca gtagagtgtg taaaagtctt
      421 ctttttaaca ccttttgaag gtggaaggca tataaaaaga gtagagaaaa tttcagcgat
      481 aattcattaa aaggaaatta aatgtcggaa tattacaaaa aatttctaaa agattcggaa
      541 caagttgcat ttgacacttt tcatcgggaa aagataaata atactattga aaagtacaat
      601 atagctgtac gtagcggaaa acgacagtat gaaaatcttg atttggcaaa aaatcgtgct
      661 gcaggcatta agcataaggt tttagagaat ttggataaat acctttatga attcgaattg
      721 aattttgaaa ataatggcgg tcgtattatt tgggcgccaa gcgctagcga cgctttaaaa
      781 gctatagttg ctatactgaa aaggaataaa gcaaaattgg ttgttaaatc aaaatcaatg
      841 attactgaag aacttgagct taacaaagtt ttggaaaatg aatctattga aacgttagag
      901 actgatttag gagagtttat agttcaggtt gccaatgaga aaccttatca tattgttaca
      961 cctgctatgc ataagtcgaa agaggaagtt gcagaattat tcagtgagaa attcggcatt
     1021 gataagagta gtacaccgga acagatttca gcttttgtaa gagaaaagct tagagataaa
     1081 tttattaatg ctgatgttgg tattacaggt gtaaattttt tgattgccga aacaggttcg
     1141 ctcgttttaa ctgaaaatga aggaaatggt gttttaacct cttcaatacc aaaaattcat
     1201 attgctattt ccggaataga aaaaataatt cctactttac gtgatcttga tctttttctg
     1261 cctttattag cagttcatgg tacaggtcaa aatataacgg cctataattc aataatatca
     1321 ggtcctaaac agaacgatga aactgatggg cctgaggaac tatatcttgt tttgttagat
     1381 aatcgccgaa cagaaatttt gaaacacaag gaattgcgaa gatctttgtc ttgtattcgt
     1441 tgtggcgcct gcttaaatgc ttgtcctgtt tacaaaaatg ttggtggata tacctataat
     1501 tctacttaca ctggtccaat tggcgctatt attactcctc atttgcgtgg aatgaatgaa
     1561 tttggtcatt taagttttgc atctactctt tgtggaaaat gtacggaggt gtgtccggta
     1621 aatatcaatt tgcatgaatt attgttgata aacagacaag agttggtgaa gtttggtatt
     1681 gttagtaaaa aagaaagaca agctcaaaaa tggacttatc gggtattaaa aaaaagaaaa
     1741 agaatggaga tagctaatgc aacgctaaaa aacagagcaa tcaacttgtt atttaaaaag
     1801 acctggggac ctcgacggac tcttcctgtt tttgctgaaa aatcctttaa taagatttgg
     1861 gaagaaaaaa acaaagaaaa gaataaaagc taactatata attactaatt agatacctaa
     1921 ttggcgttaa taatcgcaaa ttattaagta ataatttttc ttacctcgtt gagcaaggat
     1981 atacctattt tgtaaaagat cgttttcacc gatcatataa tcttctccaa ctctctcttt
     2041 gtttattgaa atagcaccat ttctcaaaaa ctctcttgct tcacgctttg atgaggaaat
     2101 ttttgtaaaa tcggtaagaa aatcaacaat tggaattgat ttataaatat catttgaact
     2161 tacattgctt tgaggcactc cttcaaaaat atttaatagc acatcttctt taagtctttt
     2221 aagtgtatcg gttgtacctt taccaaaaag aatttcactg gcatcaacgg ccaattcgta
     2281 ctcctctttt gaatgaatca ttatggtaat ttcacgtgct aaaacttttt gtaattcacg
     2341 ttggtgtggc gcttttctat gattttctat taattgatca atttcttctt ttcgtaaaag
     2401 tgtgaatatt tttatatagc ttattgcatc ttcatcggat gtgttaagcc agaattggta
     2461 aaatgaataa ggagaagttt tcgcaggatc aagccatata ttaccctctt ctgtctttcc
     2521 gaatttaccc ccgtcagctt tagtaatcaa tgggcatgtt agtgcaaatg ctttggcatc
     2581 atggccgagt tttcgtctaa ttaattccgt tcccgtgatt atatttcccc attgatccga
     2641 cccacccatc tgtaatttac agttgtgttg ctgaaataaa accagaaaat ccgttccttg
     2701 tactaattgg taagaaaatt ctgtgaacga cataccttct tttgattcag cgctaagtct
     2761 ggttttaact gaatcttttg ccatcatgta attaacagtg atatgtttcc caatatctct
     2821 aataaactgt agaaagctat actctttcat ccagtcataa ttatttacca tcaaagctcc
     2881 attaggttta ttttggttga aatcgaggaa tttagaaagt tgatttttaa ttgattcctg
     2941 attctgccgc aaagtttgtt catccaacag attacgttct ttggatttac ccgatggatc
     3001 gccaatcata ccggttgctc caccaattaa tacaataggc cgatgtccac aaacttgaaa
     3061 atgcttaagc atcattaccg aaacaagatg gcctatgtgt aatgagtctg ctgttggatc
     3121 aatacctacg taggctgttg tcatctcgtt ttccagttgt tctgtcaagc cgggagtcat
     3181 atcgtggatc attccacgcc attggagttc ttcaataaaa ttcataaatc aaattttttg
     3241 caaagataga tttatggaaa agcttggcag gattatctgt taatttatct aataaaaggc
     3301 ttaatattca aaaaacacgt cgcagagata actggataga atgattattt ttgcaaacaa
     3361 ttaatattta ataattgaaa ttatggcaga aaagatattt gattttgtaa agcctggcgt
     3421 acttacaggt aatgatgtac aaaaagtctt tgcagtagca aaaaaaaatc agtttgcatt
     3481 acctgcagtt aatgtagttg gcactgattc aataaatgca gtacttgaag cagcgcgtaa
     3541 agttaattca ccggtgatca tccaattttc taatggcgga gcatcatttt ttgctggtaa
     3601 aggattatcg aatgaaaatg aaaaggctgc aattgtagga gccatttctg gtgcgctgca
     3661 tgtccataga gttgctgaaa tgtacggagt tcctgttatt ttacataccg atcatgctgc
     3721 taagaagctt ttaccctgga ttgacggctt acttgatttt ggagaaaact tttttgaaac
     3781 ccacggtaaa cctctttata gttcacatat gctcgatctt tcggaagaac cattaaagga
     3841 gaatgttgag atatgtaaaa agtatcttga acgtatgaaa aaaattggaa tgacactcga
     3901 gatagaatta ggaattaccg gtggtgaaga ggatggcgtt gacaataccg atgttgatag
     3961 ctcaaggctt tatacccagc ctgaagaagt ggcttatgct tatgaagagt tgattaaggt
     4021 aagcaaccgt tttacaattg cagcagcctt tgggaatgta cacggagttt acaaaccagg
     4081 aaatgttaag ctaagaccaa taattctgaa aaactctcag gattatattc aaaagaagtt
     4141 taacaccgaa gttaatccaa taaatttcgt ttttcatgga ggatcaggtt cctcaagaga
     4201 agagattagg gaagctatta gctacggtgt tataaaaatg aatattgata ccgatacaca
     4261 atgggcaacc tgggaaggtg taatgaattt ttataaaaag tatcaaggtt atttacaggg
     4321 tcaaatcgga aatccggaag gtgaagacaa acccaataaa aagtattacg atccgcgtaa
     4381 gtggttacgc gaaggacaat catcaatggt taaaagacta gagcttgctt tcgaagattt
     4441 aaacaatatc aatagaaatt aaaagcaaga ttaaaagtct gttttttata taaaaatata
     4501 aacctgacag gattaatcta gcgtgttagg tttttggaag atagagcttc attaggttta
     4561 cactaaattt ataaatcagt aatattaaat cttatgaaag agattcgaat ggttgaccta
     4621 aagggtcagt atgaaaggat aaaatctgaa attgatacag caattaagga ggtgatagaa
     4681 agtactgctt ttattaatgg tcctgttgtg aaggaatttc agaatgattt agaaaattat
     4741 ctgggggtaa agcatgtaat tccatgtgga aatggtactg atgctttgca agtagccatg
     4801 atggggcttg aactaaagcc gggtgacgaa ataattacta ccacttttac ttttattgca
     4861 accgccgagg ttattgcttt gctaaaactc acaccagtac ttgtagatgt tgatccggat
     4921 acatttaata ttaaccccga tgaaattcgt aaggccatta ctcacaagac aagagctatt
     4981 gttcctgtgc atctttttgg acaatgtgcc gatatggatg aaattatgaa aattgctaag
     5041 gagcatggtt tgtatgtgat cgaagatgct tgtcaggcta ttggttctga atttattttc
     5101 gaaaatggag aaagaaaaaa ggccggtaca attggcgatg cggctggagt ctcttttttc
     5161 ccctcaaaaa atcttggatg ttttggtgat ggcggtgcaa tatttactaa taatgacaat
     5221 tttgctaaac tgatgcgtag cattgtaaac catggaatga caatacgtta ccatcacgaa
     5281 aatatcggag taaattcgag gcttgatagc attcaagcgg caattttaaa tgtaaaactc
     5341 aaatatttag acaaatacat taaagcacga caggaagcag ctaattatta tgataaaaaa
     5401 tttgcaggtc atcaaaagct taaaatacca ttcagggtta aaaattcaac acatgttttt
     5461 catcaatata ctttagtggt agatgatgta gatagagacg aactgcaaaa attcttagcc
     5521 tcgaaaaata tcccggcaat ggtttattat cccattccat tgcatttaca acaagcatat
     5581 aaagatccgc gatacaaaga gggtaatttt cctgtttcag aatatttatc aaaacatgta
     5641 atctctttgc caatgcacac cgaattagat gaagaacaat tggggtatat tacaaacact
     5701 ttgcttgaat ttattaaata aaaagttatg gagaaatcct attttgcaca cgaaactgca
     5761 gttattgacg atggatgcac aattggaaat ggcgttaaaa tttggcattt ctcgcatatt
     5821 atgaaagatt gtgtcatcgg cgagaattca aatattggac aaaatgtggt tgtatcgcca
     5881 cacgtaattc tcggtaagaa tgtcaaagta caaaacaatg tttccattta taccggtgta
     5941 atttgtgagg atgatgtttt tcttgggccg tcaatggtat ttactaatat tagtaatcca
     6001 cgtagcgctg ttgtccgtcg tgataaatat gaaaagacag ttgtaaggca aggagctacc
     6061 ataggagcaa actcaactat tgtatgcgga aataatattg gtgagtacgc atttatcgga
     6121 gccggggctg ttgtaacaaa ggaagtactt ccttatgctc ttgttgttgg taatccagca
     6181 cggcaaatcg gttggatgag cgaatatggc cacaggcttc attttgatga aaacggatac
     6241 gcaacatgcc ccgaaagcaa tgaaagatac gaattgtatg agggtaaggt aagaaaagta
     6301 aaagagtgat aatatccata aatagtacgt aaaacagatt tatttatgaa tttgtatcaa
     6361 agactaataa aaaaagaggc taaaatctcg gtaattggtt tgggttatgt cggattacct
     6421 gttgcacttg aatttgcaaa aagagttccg gtaattggat tcgatataaa atcggatcga
     6481 atagaaatga tgaaaaacgg gatagaccca agcaatgagt tatcaaaaga ggccttcgat
     6541 ggttgcgata ttttttttac agataatcca aaggatcttc aaaatgctga ttttcatatt
     6601 atagcagtac caacgccaat tgatgatagt aatatacccg atttaagccc cctgttgtcg
     6661 gcaacaaaaa cagtggctgg aatattaaaa aaaggagatt atgtagttta tgaatctact
     6721 gtgtatcctg gcgctactga agaagaagcc attcctctgt tagaagaaat ttcaggctta
     6781 aaatataaaa gagattttaa ggttggttat tcacccgaaa ggatcaaccc gggagataaa
     6841 cttcatactt tagctactgt tgtaaaggtg gtttctggat gcgatgacga atctttagaa
     6901 acgattgcaa aaatttatga aatggttgtt acggttggcg ttcataaagc cccctctatc
     6961 aaggttgccg aagcggcaaa gattatcgaa aatactcagc gtgatgttaa cattgcgctt
     7021 atgaatgaac tttcgatcat ctttaaccgt atgggtataa atacttatga tgtgctggag
     7081 gccgcaggaa caaaatggaa tttcttaaac ttttacccag gacttgtagg aggacattgt
     7141 attggtgttg acccatatta ccttgcctat aaggcaaaaa aatatagata ccatccacag
     7201 gtgataaatt cagggcgttt tgtaaatgat tcgatggggt tttacgtagc aaagcaagcg
     7261 gtaaaaaaaa tgataaacgc taaaaaaaat atttccgact gtaaagtatt agtcatggga
     7321 ttaacattta aagaagatgt tagcgatata cgaaactctc gggtgcccga tattatcacc
     7381 gaactgaaat catacgctgt taatgttgac gtaatggatc ctcttgctga taatgaagag
     7441 gtaaaaaagg aatatgggtt tgaattgatt agttcgataa ctaacaagta tgatgctgtt
     7501 attgttgctg ttaatcattt aaaatacaag aatttgaatg aggatttttt taaatcaata
     7561 actacgcaaa atgcaatttt aattgatgtg aaaggaattt acaggaataa aatcaaacat
     7621 ttaaactatt ggagtttata gtttcatttg atttttaaag aaaacacaaa atgggtctgc
     7681 tctgtaattt tcgtcaaaaa tttgattaac tttgggcaca attttaattt tatggttatc
     7741 agatattagt tctttatgaa aaaagtgctt gttacagggg gtacaggata cattggatcg
     7801 catactgttg ttgagcttca gcaagttggg tatgatgtaa ttattgttga taacctctcc
     7861 aattctcagc ctgacgtttt ggaaaacata actaacataa ccggtataaa gatagacttt
     7921 gaaaagtttg acctggcaga tcgaaagaag acgcttgatt tttttaaacg ccataaaaat
     7981 atagatgcaa ttattcattt tgcagcatta aaagctgtag gggaatctgt cgttcagcct
     8041 ctaaattatt attataacaa tcttttttcg ctgatgaata ttttgcaagg gatgttggat
     8101 aataatattg aaaacttagt tttttcatca tcctgtactg tttacggaca acctgctaac
     8161 ttaccggttg atgaaagcgc accggttcag attgcattat ctccttatgg caatacaaaa
     8221 ca
//
LOCUS       DAOOAI010000095         8129 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_10713_length_8129_cov_4.489101, whole genome shotgun sequence.
ACCESSION   DAOOAI010000095 DAOOAI010000000
VERSION     DAOOAI010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 8129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8129
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10713_length_8129_cov_4.489101"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1006
                     /gene="groEL"
                     /locus_tag="PK345_07180"
     CDS             <1..1006
                     /gene="groEL"
                     /locus_tag="PK345_07180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HPX34834.1"
                     /translation="AVYENPFILIHDKKISVMKDLLPILEKVVQTGKPLLIISEDVET
                     EALATLVVNKLRGGLKVVAVKAPGFGDRRKEMLEDIAILTGGTVISEEKGYKLEDTVL
                     EYLGQAEKITVDKENTTIVSGKGSKANIDGRVNQIKKQIETTTSDYDKEKLQERLAKL
                     AGGVAVIYVGAASEVEMKEKKDRFDDALNATRAAIEEGIIPGGGVGYLRAISALDGLK
                     VDNEDQNTGIAILKRALEEPLRQIVENAGMEGSVVVNKVKEGKGDFGFNAATEVYENL
                     YDAGVIDPTKVARVALENASSIAGMLLTTECVLAEHKDEKEPAGMPGGMPPGGMGGMY
                     "
     gene            complement(1154..2830)
                     /gene="ettA"
                     /locus_tag="PK345_07185"
     CDS             complement(1154..2830)
                     /gene="ettA"
                     /locus_tag="PK345_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010259685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0045900 - negative regulation of
                     translational elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HPX34835.1"
                     /translation="MADDNKIIFSMIGVGKLIPPNKQILKDIYLSFFYGAKIGIIGLN
                     GSGKSTLLKIIAGHDTSYQGKIAFSPGYSIGLLEQEPYLDNTKTVREVVEEGVQETVD
                     LLKEYEALNLKFAEPMSDDEMSALIEKQGKLTDLIDQHGAWELDSKLERAMDVLRCPI
                     GTAPVKHLSGGERRRVALCRLLLKEPDILLLDEPTNHLDAESVEWLEQHLKEYKGTVI
                     AVTHDRYFLDNVAGWILELDRGEGIPWRGNYSSWLEQKSNRLKMEEKAESKRRKTLER
                     ELEWVRMSPKARHAKAKARLSAYDKLLNEDVKQKEESLEIFIPNGPRLGNNVIEAIDV
                     SKAFGDKVLFEQLTFSLPPAGIVGIIGPNGAGKTTLFRMIIGSEKPDTGAFRIGESVK
                     IGYVDQSHAEIDPEKTVWQVISEGQDTIQLGNRSMNSRAYVARFNFSGADQEKKTGVL
                     SGGERNRLHLALTLRQEANVLLLDEPTNDIDVNTLRALEEGLENFAGCAVIISHDRWF
                     LDRVATHILAFEGDSQVYYFEGNYSEYEENRKKRLGSDTPHRIKYKKLKA"
     gene            complement(2938..3729)
                     /locus_tag="PK345_07190"
     CDS             complement(2938..3729)
                     /locus_tag="PK345_07190"
                     /inference="COORDINATES: protein motif:HMM:NF016241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF481 domain-containing protein"
                     /protein_id="HPX34836.1"
                     /translation="MRQFFVFPSLFILSAILLITQPLFSQVNTEKFRKYDVEKGFQAN
                     LQTTFTTKAGNTHYNTLKATSRLDYSATKFDYFLVANFEAKSASKKRIENEGFIHLRG
                     MWKFADKANWEYFAQRQYDEFIDLRFRNLLGTAIKYRILKAVSKNDSNSMILINASTG
                     LMYEHENYKYEPTNIDNKLIRSTNFISFDWSKNESTSFTGVIYYQPAFNDRKNFRIVA
                     ESTLEFNIIKRVFFSINFVYKYNNHPFTDVKPYDISLENGLRLKL"
     gene            complement(3759..4286)
                     /locus_tag="PK345_07195"
     CDS             complement(3759..4286)
                     /locus_tag="PK345_07195"
                     /inference="COORDINATES: protein motif:HMM:NF015674.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HPX34837.1"
                     /translation="MKNTNQFRSLLVINGIIAFLFGLFAIFAPLETAKTIALYFGLIL
                     IGASVVGIIISVKKINQKQLIPYNLLSSVLSLIIGIFIVVYTKKSLELFTIIVGIWAI
                     LIAVLQIVILIQIIKRSVYRKVMIFNSIITLIFGIILFTNPLESIAAMVYITGALAIV
                     FGSILIYIAIKIKSL"
     gene            complement(4308..5477)
                     /locus_tag="PK345_07200"
     CDS             complement(4308..5477)
                     /locus_tag="PK345_07200"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HPX34838.1"
                     /translation="MKLLVILPRVPYPLEKGDKLRAYNQIRFLSKTNEITLCALNDIK
                     LHPNAKSELKPYCKRLYILNISKWSIILNLFKALFKGIPFQVGYFYNRKAKQQIQQII
                     NEVKPDHIYCQLIRVAEYVKNNSTPKTIDYQDVFSKGVERRIATSPFYLRPLLRMEYK
                     RLLKYECNIFDIFNNKTIISAPDRDLIPHPERKKIHIIPNGVDTEFFKPMDVEKQFEI
                     VFIGNMGYPPNVDAAEYLAKEILPQVKKRYPDARLLLAGATPTQRVKNLENESVVVTG
                     WVDDIRQCYAQAKIFIAPMRIGTGLQNKLLEAMAMKIPCITSPLAFSALNAKKNEDIL
                     VGENVEEFSNLIVNLLNDNTLSAKLAEAGYQFVLKNYSWEMNTMELDTIMKNTAI"
     gene            complement(5501..6709)
                     /locus_tag="PK345_07205"
     CDS             complement(5501..6709)
                     /locus_tag="PK345_07205"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPX34839.1"
                     /translation="MKILILCNKSPYPPKEGGPIAMNAIIEGLIEAGHQVKVLAINTN
                     KYFIKENDIPESYRQKTGIETVYIDLSIKPLDALLNLFSKDSYHVQRFISKEFEKKLI
                     SILQNDTFDIVQLETLYISPFIETIRENSKAKIVLRAHNIEHLIWERVAEISSNPLKK
                     LYLKHLAKKLKNYELEIISKYDGIATITSKDASSFKNTGCEVPIISIPFGIDLEKIKE
                     KPGIKAEFPSLFHIGAMNWMPNIEGIKWFLDKVWPLIQPEFPNLKFYIAGRSMPSWLY
                     QKQLPNVEIVGEVPDAFDFMYSKAIEIVPLFSGSGIRIKIIEGMAVAKAIISTTIGAE
                     GINVTHEKNILLADDPIAFANAVKRCVNDEQFVLSLGTNARELIRNEHSNKQIIEKLT
                     KFYHSLVLPQ"
     gene            complement(6712..>8129)
                     /locus_tag="PK345_07210"
     CDS             complement(6712..>8129)
                     /locus_tag="PK345_07210"
                     /inference="COORDINATES: protein motif:HMM:NF015684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LptF/LptG family permease"
                     /protein_id="HPX34840.1"
                     /translation="YWFIIKSYIGPLLFTFFIALFILVMQFLWKYVDELVGKGLELHI
                     LLELLFYASSTFVPLALPLAILLSSLMTFGNFGEHYELVAMKSTGIALRRIMMPLIIL
                     SIFISGFAFFFSNNVLPIANLKFHALLYDVRQKKLTFNIQEGVFYNGIEGFTIYVREK
                     EPDKNLLRDVMIYNHSDRMGNMRLTTAKYGTMALSANQSMLVFNLYDGHNYEEITDRQ
                     NYQITRPFQRISFSEQTRTFSGLDFGMNRTDETLFRNNYQMMNLDQLTHATDSLKNDL
                     ETRKENLNNIVNERFSHLHQVESLINKENNKNVSFSLNEVDTASMIKATDIALRSSRQ
                     IKNQIEYNRKDIEGRKKRIIRHELEWHRKFTLSIACLILFFIGAPLGAIIRKGGLGMP
                     VVFSIIFFVIFHVISISGEKYARAGVMDPAIAMWLASLVLLPLGIFLTYKATTDSPLL
                     DADVWKRIFKLMLLKSDNKTN"
BASE COUNT         2430 a         1455 c         1585 g         2659 t
ORIGIN      
        1 agcagtgtac gaaaatcctt ttatcttaat tcatgacaag aaaatctcag tgatgaagga
       61 tttgctccct atccttgaaa aagttgttca aactggtaaa cctttgctta ttatttccga
      121 agatgtcgaa accgaagcgc tagcaacact tgttgtgaat aagcttcgtg ggggattaaa
      181 agtagtagct gttaaagcgc ctggatttgg agataggaga aaagagatgc ttgaagatat
      241 agcaatttta acaggtggaa cagttatttc ggaagaaaaa ggatataaac tcgaggatac
      301 agtattggaa tatctcggtc aggcagaaaa gataacagtg gataaagaga atactaccat
      361 tgttagtggt aaaggctcaa aagccaatat tgatggccgg gttaatcaga taaaaaaaca
      421 gattgaaact accacctcag attatgataa agaaaagctg caagagagac ttgcgaagct
      481 tgccggtggc gtagcagtaa tttatgttgg agcggctagc gaagttgaaa tgaaagagaa
      541 gaaagatcgt tttgacgatg ctttaaacgc tacacgtgct gctatcgaag aggggataat
      601 tcccggtggc ggtgtcggct acttacgtgc tataagtgca cttgacggct taaaagtgga
      661 taacgaagat cagaatactg gtatcgcaat ccttaaaaga gccttggaag agccattacg
      721 tcagatagtt gaaaatgctg gaatggaagg atcggtggta gttaataagg tgaaggaagg
      781 aaagggtgat tttggtttta atgctgctac tgaagtttat gaaaaccttt atgatgcagg
      841 ggtaattgat cctacaaaag tagctcgcgt agctcttgaa aatgcatctt ccattgccgg
      901 tatgttgttg actaccgaat gtgttcttgc cgaacataaa gatgaaaaag aacccgctgg
      961 catgcctggt ggaatgcccc caggtggcat gggtggaatg tattaaaata agaattgctc
     1021 aaattttaag aaaagctctc cacttggggg gctttttttt ttgaaataaa accaaaatac
     1081 ctacacggat aattagaata aaaagtcaaa aaaaattgag ggttctaata ctaaacttaa
     1141 tggtatttta tttttaggct tttagttttt tgtatttaat cctgtgagga gtatcggaac
     1201 caagtcgttt tttgcgattt tcttcatatt ccgaataatt tccttcgaaa tagtaaactt
     1261 gcgaatcgcc ttcaaaagca agtatatgag tagctacccg gtcgagaaac cacctatcgt
     1321 gcgaaataat tacagcacaa cctgcaaaat tttccaaacc ctcttctaag gctcttaggg
     1381 tatttacatc aatatcgttg gtgggttcgt cgagtagtag tacgttagct tcctgcctta
     1441 atgttaatgc cagatgcaag cggtttcgtt ctccacccga tagcactccg gtttttttct
     1501 cctgatccgc gccactaaaa ttaaatcgtg caacgtaggc acgtgaattc attgaccggt
     1561 tgccaagttg aatagtgtcc tgtccttccg aaatgacttg ccatacggtt ttttcagggt
     1621 caatttcggc atgggattga tccacataac cgattttaac tgattcgcca attcgaaacg
     1681 ctccggtgtc aggtttttcg cttcctatta tcattctgaa aagtgttgtt ttaccagctc
     1741 catttggccc tataataccc actatacctg ctggtggcaa agaaaaggtc aactgctcaa
     1801 aaagtacttt atccccaaaa gcttttgaaa catcaatggc ttcaatcaca ttatttccca
     1861 atctggggcc gttggggata aatatttcga ggctctcttc tttttgtttt acatcttcat
     1921 taagtaattt atcgtaggct gagagccttg ctttcgcttt agcatgacgc gctttagggc
     1981 tcattcgtac ccattcaagt tcacgttcta atgtttttct acgcttgctt tctgccttct
     2041 cttccatttt taggcggttt gatttctgtt caagccatga tgagtaattc cccctccatg
     2101 gaatgccttc gccacggtcg agttcaagta tccagccagc tacgttatca agaaaatatc
     2161 ggtcgtgcgt aacggcgatg acggtacctt tgtattcttt aagatgctgt tcaagccact
     2221 ctaccgactc agcgtcaagg tggttggttg gctcatcaag tagtaaaatg tcaggttctt
     2281 taagtaaaag acgacatagt gccactctgc ggcgctcgcc acctgataga tgcttaacag
     2341 gcgcagtgcc tattggacaa cgcaatacat ccatcgcacg ttcgagtttc gaatcaagct
     2401 cccacgcccc atgctggtca atgagatcgg tcagcttgcc ttgcttttct attaaggcgc
     2461 tcatctcatc atcactcatc ggttcggcaa acttaagatt aagagcttca tattctttta
     2521 ataaatcaac tgtttcctga acaccctctt ctacaacctc tcttacagtt ttagtattgt
     2581 cgagatatgg ctcctgctca agtaaaccga tggaataacc aggagaaaat gctattttac
     2641 cctgatatga tgtgtcatga ccggcaataa tttttaaaag agttgactta ccagagccat
     2701 ttaatccgat aatgccgatt ttggcgccat aaaaaaagga gaggtaaata tcttttaaaa
     2761 tttgtttgtt gggagggata agtttaccaa ccccaatcat tgagaaaata atcttattat
     2821 cgtctgccat aactaattaa atataaagat gatgtgtgtt tgtcaaaagt aataaaattc
     2881 cctattgtaa gccatattat ctgaacaagt agagtagcat agcattttta gaggcgacta
     2941 aagtttcagc ctaagcccat tttcaaggga aatgtcgtaa ggctttacat cagtaaatgg
     3001 atgattattg tatttgtaaa caaaattaat tgagaaaaat actcttttta taatgttaaa
     3061 ttcaagtgta ctttcagcta caattctaaa gttttttcta tcattaaacg ctggttggta
     3121 gtatataacg ccggtaaagc tcgttgattc attttttgac cagtcgaaac ttataaaatt
     3181 cgttgatctt attaatttgt tgtcaatatt tgtcggttcg tatttgtaat tctcatgttc
     3241 gtacattagt cctgtacttg cattaataag gatcattgaa ttagagtcat ttttcgatac
     3301 cgctttcaaa atcctatatt ttattgcagt tcctaataaa tttctgaatc taagatctat
     3361 aaattcatca tattgacgtt gtgcaaaata ctcccaatta gctttatctg caaatttcca
     3421 cattccgcga aggtgaataa aaccttcgtt ttcgatgcgt tttttgctgg ctgatttagc
     3481 ttcgaaattt gctacaagaa aataatcaaa ttttgtagcc gaataatcaa gacggcttgt
     3541 agcttttaga gtattgtaat gtgtgtttcc tgcttttgtg gtgaaggtag tttgtagatt
     3601 agcctgaaat cccttctcaa catcatactt cctgaatttt tcagtattta cctggctgaa
     3661 aagaggctga gtaattaata aaattgcact gagaatgaat aaacttggaa aaacaaaaaa
     3721 ttgtcgcaca atgataaatc atttctaatt aataaaattt ataaactttt gattttaata
     3781 gctatgtaaa tcaagatgct gccaaacact attgcaagtg ctccggtaat gtaaaccatg
     3841 gcggcaatgg actctaaagg attggtaaac aatataatgc caaaaattag agtgataatg
     3901 ctattgaaaa tcattacttt gcggtaaact gaccttttaa ttatttgaat caggattact
     3961 atttgcaaga cggcgataag aatagcccag atacctacta tgattgtaaa aagttcgaga
     4021 ctcttttttg tgtaaaccac aataaaaata ccgatgatca aactaagtac tgaacttaac
     4081 agattatatg ggataagttg tttttgattt atttttttta ctgaaattat aattcctacc
     4141 acactggcac caataaggat taaaccgaaa tatagagcta tagttttggc agtttctaaa
     4201 ggtgcaaaaa ttgcaaaaag tccaaagaga aaagctataa tcccgttaat cactaacaag
     4261 gagcgaaatt gatttgtgtt tttcatggaa gaaccttttt atgattttta gatagcggta
     4321 tttttcataa ttgtatccaa ctccatagtg ttcatctccc aactatagtt tttaagtaca
     4381 aattgatacc ctgcttctgc taatttagct gatagcgtat tgtcatttaa caaattgaca
     4441 ataaggttag aaaactcttc aacattttct ccaacaagaa tatcttcgtt tttcttggca
     4501 ttcagtgcac tgaatgccaa gggagatgtt atacatggta ttttcatggc cattgcttcc
     4561 agcagcttat tctgcaatcc tgttccgata cgcattggag ctatgaagat cttagcctga
     4621 gcgtagcatt gtcttatatc gtccacccat cccgttacaa caactgattc gttttcaaga
     4681 ttttttacac gctgggtcgg tgttgcgccg gcaagtagca atcgggcatc gggatatcgc
     4741 ttttttactt gcggtaaaat ttcttttgca aggtattcgg cagcatcaac gtttggagga
     4801 tatcccatat taccgataaa cactatctca aattgttttt cgacatccat cggcttgaaa
     4861 aactcagtat ccacaccatt gggtatgata tgtatttttt ttctttcagg atgcggtatc
     4921 aagtcacggt cgggagcaga aatgatggtt ttattgttga aaatatcgaa gatgttacat
     4981 tcatatttta gtagtctttt atattccatt cgtaacagtg gccttaggta aaatggtgaa
     5041 gtggcaatac gccgttctac accttttgaa aaaacatcct gataatcaat ggttttgggt
     5101 gttgagttgt ttttaacgta ttcagctact ctgattaatt gacaataaat atgatcaggt
     5161 ttaacctcgt ttatgatttg ttgtatctgc tgttttgctt ttcggttata aaaatatccg
     5221 acttgaaagg gtatcccttt aaacagagcc ttgaaaagat tgaggatgat gctccattta
     5281 ctgatattta atatgtataa ccttttgcaa taaggtttta attcgctttt ggcatttgga
     5341 tgcaacttta tgtcgtttag tgcgcaaagc gtaatttcat tcgttttcga caaaaatctt
     5401 atctggttgt aagctcgaag cttatctccc ttttcgagag ggtaggggac gcgtggtaat
     5461 attacaagta atttcatttg acaaagataa tcgttagggt ttattgtggt agtacaagtg
     5521 aatgataaaa tttagtaagc ttttctataa tttgtttatt tgaatgttca tttctgatca
     5581 gttcacgggc attagtgcca agcgacagca caaattgctc atcgtttaca cagcgtttta
     5641 cagcatttgc aaaagctatt gggtcgtcag ctaaaagaat attcttttca tgggtaacgt
     5701 ttattccttc tgcgccaatt gtagttgaga taatagcttt tgctacagcc attccttcga
     5761 tgatcttgat ccgtattccg cttcccgaaa aaagtggtac aatttcaata gctttagaat
     5821 acatgaaatc gaatgcatca ggcacctctc caactatttc cacattgggt aattgctttt
     5881 gatataacca tgaaggcatt gatctgccgg caatgtaaaa ctttaggtta ggaaattctg
     5941 gttgtatcaa aggccatact ttatcgagaa accattttat gccctctata ttcggcatcc
     6001 aattcattgc accgatatgg aataatgaag gaaactcagc cttaatacca ggtttttctt
     6061 ttattttttc tagatcaatc ccaaatggta tagagattat aggaacttcg caacctgtat
     6121 ttttaaaaga gcttgcatct ttacttgtga ttgtggcaat accatcgtat ttggagatta
     6181 tttcaagctc gtaatttttt aacttttttg caagatgttt caggtagagt tttttgagtg
     6241 gattagaact gatttccgct acacgttccc atatcagatg ctcgatatta tgagctcgta
     6301 atacgatttt agccttggaa ttctctctta ttgtttctat aaaggggcta atgtatagcg
     6361 tttcaagctg aactatatca aaagtgtcgt tttgcaggat agagattagc tttttttcaa
     6421 attctttact aatgaaacgt tgaacatggt acgaatcttt actgaaaaga ttaagcagag
     6481 catctaaagg ttttattgaa agatcaatat aaactgtttc aatacctgtt ttctgtcggt
     6541 aactttcggg tatgtcattt tctttaataa agtatttgtt tgtattaatt gcaagcactt
     6601 taacttgatg gccggcttca attaatcctt caattattgc gttcatggca ataggccctc
     6661 cctctttagg cggataggga gatttgttgc agagaatcag tattttcatt gtcaattagt
     6721 tttgttatca gattttaaca gcattaattt aaatattctt ttccacacat ctgcgtcaag
     6781 taatggcgaa tctgtagtgg ctttgtatgt aagaaatatt ccaagcggta gcaacaccaa
     6841 tgatgctaac cacatggcaa ttgcgggatc cattacgcct gcgcgtgcat atttctcacc
     6901 cgaaatagat ataacatgaa aaattacaaa aaagatgatt gaaaacacta caggcatgcc
     6961 aagcccccct ttgcgaatta tggctccaag cggtgcgccg ataaaaaaca gtatgagaca
     7021 tgctatggaa agtgtaaatt ttcgatgcca ttctaactcg tgacgaatta ttcttttctt
     7081 acggccttca atatcttttc gattgtattc tatctgattt tttatctgac gtgatgatct
     7141 taatgcaata tcagttgctt taatcattga ggccgtatct acttcattta gtgaaaagct
     7201 aacatttttg ttattttctt tattaattaa ggattcaact tgatggagat gtgaaaatcg
     7261 ttcgttcact atgttattta ggttctcttt ccgcgtctcg agatcatttt taagagagtc
     7321 ggtagcatgg gttaactgat cgagattcat catttgatag ttgtttcgga aaagtgtttc
     7381 gtcagtacgg ttcatgccaa agtcgaggcc actaaatgta cgggtttgct cggaaaaaga
     7441 aatacgttgg aatggcctgg ttatctgata attttgcctg tcggtgatct cttcataatt
     7501 atgcccgtca taaagattaa aaacgagcat ggattggttg gcagaaagag ccattgtgcc
     7561 atattttgct gttgttaatc tcatgttacc cattcggtcg gaatgattgt aaatcatcac
     7621 gtcccgaagg aggtttttat ccggttcttt ttctcttaca taaatggtga agccctcgat
     7681 tccattatag aaaacacctt cctgaatatt gaaagtgagt tttttttgcc ttacgtcata
     7741 aagcaaagcg tggaatttaa ggttggcgat aggtaaaaca ttgttggaaa agaaaaaggc
     7801 aaatccgctg atgaagattg aaagtatgat taacggcatc atgatacgcc gcaaggcaat
     7861 gcccgttgac ttcatggcca ccaattcgta atgttcgcca aaattgccaa atgtcatcag
     7921 tgacgaaagc aggatagcca aaggaagcgc taaaggaaca aaagtactcg atgcataaaa
     7981 gagaagttcg agtagtatat gtagttcaag ccctttacca actaattcat caacgtactt
     8041 ccacaaaaat tgcataacaa gaatgaagag agctatgaaa aaggtaaata gcagaggacc
     8101 gatataggat ttaataataa accaataga
//
LOCUS       DAOOAI010000096         7955 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_10979_length_7955_cov_5.766203, whole genome shotgun sequence.
ACCESSION   DAOOAI010000096 DAOOAI010000000
VERSION     DAOOAI010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7955
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10979_length_7955_cov_5.766203"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="PK345_07215"
     CDS             complement(<1..1288)
                     /locus_tag="PK345_07215"
                     /inference="COORDINATES: protein motif:HMM:NF019850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HPX34841.1"
                     /translation="MRVHFIAIGGAVMHNLAIALVNKGYAVTGSDDEIFEPALSNLRN
                     YGILPDKFGWYPEKITSDLDAVILGMHAKAGNPELDKAVELGLKIYSFPEYIFQQSIN
                     KTRIVIAGSHGKTTITSMIMHVLKSQGIDFDYLVGAKIEGFDIMVRLSDAPFIVIEGD
                     EYLTSALDRRPKFLHYKPKIALISGIAWDHINVFPTFEEYKEQFQFFIQSIENDGYLI
                     FCDDDKTLSEVVKSSNWGGKVIPYNLLDFETKDGKTRIILKGDPPKYYPINFFGKHNL
                     LNLSGAKEICKLLGINESDFYQSIISFKGAANRLQLVAENEHTTIFKDFAHAPSKLKA
                     TVEAVNLQFQHRKLIAVMELHTYSSLSKNFLAHYRGSMDQADIPVIFFDPAAIALKKL
                     PPITIEDVFEGFGNKKLKIFTNPLQLQEFILQQNYVN"
     gene            complement(1300..2070)
                     /locus_tag="PK345_07220"
     CDS             complement(1300..2070)
                     /locus_tag="PK345_07220"
                     /inference="COORDINATES: protein motif:HMM:NF015962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-phosphosulfolactate phosphatase"
                     /protein_id="HPX34842.1"
                     /translation="MSFFKDMIQKSCGGVPIEVCFSPALIGFLNIKTEAIVVVTDILR
                     ASTTMVSALADGATSIIPVALVEQAKFLKQQGFLVVAERGGAKLDFADFGNSAFEFRD
                     GKSKGEKIVFTTTNGTAAINEAKERGTVAIGAFSNLSVLAEWLTNQNKQVIILCSGWE
                     NNFCLEDTVFAGALIEKLQSTGKFFINNNDSAHAALDLWNIAKKDILTYINKAQHRLR
                     LQKLGADDVIEFSFTPDTAKVIPVLIENELIDILKPDL"
     gene            complement(2080..3171)
                     /gene="gcvT"
                     /locus_tag="PK345_07225"
     CDS             complement(2080..3171)
                     /gene="gcvT"
                     /locus_tag="PK345_07225"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004289780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HPX34843.1"
                     /translation="MKNTAFIRMHEALGAKIVPFAGFNMPIQFEGVNAEHETVRKAVG
                     VFDVSHMGEIWVRGPKAVELLQKLLTNDVSKLVDGKIQYTCFTNHLGGIVDDLLLYRF
                     DAENYLLAINASNIEKDWKWMQENNDVGAIIENASDTTSQLAIQGPKAMATLQKLTNV
                     DLTTIPYYTFKVIEFAGLKDVIMSATGYTGEKNSYEIYFPNEYAGKIWNAVFEAGAEF
                     GIKPIGLGARDTLRLEMGFCLYGNDINETTSPIEAGLGWITKFVEGKDFINRAYHENL
                     KINGVTRRLRGFEMIDKGIPRQHYEICDADGNIIGKVTSGTMSPSLKKAIGMGYLKTG
                     FDAFGQEIYIKIREKLLKAKVVKTPFYEG"
     gene            complement(3504..4559)
                     /gene="sucC"
                     /locus_tag="PK345_07230"
     CDS             complement(3504..4559)
                     /gene="sucC"
                     /locus_tag="PK345_07230"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HPX34844.1"
                     /translation="MGCKSPDTCWRTRKRGWSELAKNVREVEKFTDKIIGMNLITPQT
                     PPEGKKVSKVLIQQDVFYPGPSDIREFYISVLLNRKTGRNMIMYSPIGGMDIEAVAEK
                     TPDQIFTEEIDPRYGLQGFQARRVAFNLSLEGDAFKNMVKFVQSLYKAYLDSDATLFE
                     INPVLKASDDKIFAVDAKVTIDNNALYRHPDIAQMRDLAEEDPIEVEASRYDLNYVKL
                     NGNVGCMVNGAGLAMATMDMIKMSGGDPANFLDVGGTANAKRVEEGFRIILKDPNVKA
                     ILVNIFGGIVRCDRVAQGIIDAYNNIDNINVPIIVRLQGTNAEEAKILLDESGLEVRS
                     AIALEDAARLVNEALSE"
     gene            complement(4499..4696)
                     /locus_tag="PK345_07235"
     CDS             complement(4499..4696)
                     /locus_tag="PK345_07235"
                     /inference="COORDINATES: protein motif:HMM:NF020031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34845.1"
                     /translation="MDLHEFQGKSILKKYGVSVPEGILAESPEQAVEAAKHLHAITGT
                     KFWVVKAQIHAGGRGKGGGVN"
     gene            5318..>7955
                     /locus_tag="PK345_07240"
     CDS             5318..>7955
                     /locus_tag="PK345_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HPX34846.1"
                     /translation="MKSCLVSFFVFIFFFFQHNPFSLNAQEASGLESNQYIVRTAERP
                     PIDLLQIPVEAYEAGKFKIKLKPERENLIPVLSSSTSPVGYVVTGIPSFDALNQYYSI
                     KSYRPLFGHLYHTRSDSDQFMERHKAWGFHLWLEVIVDDKTDIIDAVMQFSALSEVDF
                     AEPEYRKRLVINDAKFNSANLVISNENEPEGRWTPNDPQYGNQWHYKNTGQQGGTPGA
                     DISLQEAWNIEKGNSNVIVAVIDGGISYTHQDLASNMWSEIGYNFVNGSPTVTPHDHG
                     THVAGTIAAVTSNMIGVAGVAGGSGTGDGVRLMSCQVFSNNGSGGFHLAPIYAADNGA
                     SISQNSWGYSNAGVYDQSVLDAIDYFNVYGGGEAINGGITLFSAGNSNSPGQWYPACY
                     SGCFSVAATNNQDKKAWYSNYDTWVDISAPGGETNSVTARGVLSTLPNNSYGYFQGTS
                     MACPHVSGVVALMISLGYGELTSLQIDSILRNTTDNHYNINSNYLGKLGSGRLNAHSA
                     LLAVQELVQQVNNPQSFKASAINANRINLTWVKNTSNNNVMVVWSLNNVFGTPIDGNV
                     YNVGTTLPGGGKVLYRGSNTNFSHLNLQPLTTYYYKAFSFNSANEYSTGITASATTLM
                     QPYADFYANPTTQVVSLPITFTDSSNGATFSSWFWDFGEGANPATTTGKGPHQVVYNT
                     PGSKTVTLVVDNNYTKIKENYINILSDCYSISETYSNGDIQTDLNFQTLPGSSSCPGS
                     LIVSIPLGAVILGVDVSYNMTARNNGWKSEQRSQLKCVSPGGLSEPELYSGSGNSTGT
                     LSYNRNGLNIANGVEGGGDIHFQLHAGRTFGGSGCNTVYNKVDNNTWNITVYYYLLEL
                     ENPPIADFTATPTTVYAGETVSF"
BASE COUNT         2423 a         1548 c         1438 g         2546 t
ORIGIN      
        1 agtttacata attttgttgg aggatgaatt cttgaagctg caaaggattc gtaaatattt
       61 ttagtttttt gttaccaaag ccttcaaata catcttcaat cgtgattggt ggaagttttt
      121 ttaatgcaat ggccgcagga tcaaaaaata ttaccggaat atctgcctga tccatgctac
      181 ctcgataatg agcaagaaag tttttactta aactactata agtatgcaac tccattactg
      241 ctatgagttt acgatgctgg aactgtaaat taacagcctc tacagtagct tttaacttcg
      301 agggagcgtg ggcaaaatct ttaaaaatgg tagtatgttc gttttcagca accaactgca
      361 acctgttagc tgccccttta aaactaatga ttgactgata gaaatcggac tcattaatgc
      421 cgagcaactt acatatttct ttggcaccac tgagattcaa taaattatgt ttcccaaaaa
      481 aattgattgg gtaatattta ggaggatctc cttttaggat aattcgggtt ttaccatctt
      541 ttgtttcaaa atcgagaaga ttatatggta tcactttccc tccccaattg gaacttttga
      601 ctacctcgct cagtgtttta tcatcatcac aaaatataag gtatccatcg ttttcaatac
      661 tttgaataaa aaactgaaat tgttccttgt actcctcaaa agtgggaaac acatttatgt
      721 gatcccaggc aataccactg ataagtgcaa ttttcggttt ataatgcagg aatttaggtc
      781 ttcgatccaa tgcagaagtc aaatattcgt cgccttcgat aactataaaa ggggcatcag
      841 aaagccttac catgatatcg aacccctcaa ttttagctcc gactaaataa tcaaaatcta
      901 ttccttgact tttaagcaca tgcataatca tggaagtgat agtagtcttt ccatgactgc
      961 cggcaataac aattcttgtt ttattaattg actgctggaa aatatactca gggaaagagt
     1021 agattttaag cccaagttca acggctttgt ctaattcggg gtttccagct ttagcatgca
     1081 tgccaagtat gactgcgtcg aggtcagaag ttattttttc aggataccaa ccgaatttgt
     1141 cgggcaggat tccataattt cttaagttcg acagtgcagg ttcaaatatt tcgtcatccg
     1201 atccggttac agcatatcct ttatttacta atgctatggc cagattatgc ataacagctc
     1261 cgccaatggc aataaaatgg actctcataa tattaatttt taaagatcgg gttttaaaat
     1321 atcaatcagc tcattttcaa ttaaaaccgg tataacctta gctgtatcgg gagtaaacga
     1381 aaattcgatg acatcgtcgg cgccaagttt ttgtaatcgt aagcgatgct gtgctttatt
     1441 tatatatgtt aaaatatcct ttttggcaat attccataga tcaagcgctg catgagctga
     1501 gtcgttatta ttaataaaaa atttccccgt tgattgtagt ttttcaatca aagccccagc
     1561 aaaaacggta tcttcaaggc agaaattatt ttcccaacca gagcaaagta tgattacttg
     1621 tttattttga ttagtaagcc attcggctaa tacagataaa ttgctgaagg cgcctattgc
     1681 aacggttccc ctctctttgg cttcatttat tgctgctgta ccgttggtag tggtaaaaac
     1741 tatcttctca ccttttgatt ttccatctct aaactcaaag gctgagttgc caaaatcggc
     1801 aaaatcgagc tttgcaccgc ctctttcagc tactaccaaa aagccctgtt gctttaaaaa
     1861 cttagcttgc tcaactaatg caacggggat tatagaagtg gctccatcag caagcgcaga
     1921 aaccatcgtt gttgaagccc gtaaaatatc cgttactaca acaatagctt ctgttttgat
     1981 atttaaaaaa ccaataagcg ccggcgaaaa acaaacttcg ataggtactc caccacagga
     2041 tttttggatc atgtccttaa aaaaactcaa tattatgatt tatccttcgt aaaagggtgt
     2101 tttaaccact ttggctttta gcagcttctc tctgattttg atatatattt cctgtccgaa
     2161 agcatcaaaa ccggttttta aatatcccat gccgatagct tttttaagcg aaggcgacat
     2221 cgtgcctgag gtaactttac caataatgtt tccatcggca tcgcatattt cgtagtgctg
     2281 ccgagggatg cctttatcaa tcatttcgaa gccacgcaat cttcgggtta caccattaat
     2341 ttttaggttt tcgtgataag ctctgttgat aaaatcttta ccttcaacaa atttggttat
     2401 ccatcccaat ccagcttcta ttggcgaggt tgtttcgtta atatcgttac cgtacaagca
     2461 aaatcccatc tcaagcctca atgtgtcgcg cgctcctaat ccgatgggtt taatgccaaa
     2521 ttcggcgccg gcttcaaaaa cagcattcca tatcttcccg gcatattcat tagggaaata
     2581 aatctcataa ctgtttttct ctcccgtata accggttgct gacattatta catcctttag
     2641 tccggcaaat tctatcactt taaaagtgta ataaggtatt gtggtaaggt caacattggt
     2701 aagtttctgt aaagttgcca tagcttttgg cccttgtata gcgagttgcg aagtggtatc
     2761 cgaagcattt tctataattg ctcctacatc attgttttcc tgcatccact tccagtcttt
     2821 ttcgatatta gaagcgttta tggccaacag ataattttcg gcgtcaaaac ggtacagtaa
     2881 taaatcgtca actattcctc ccagatggtt ggtaaagcag gtatattgaa ttttgccatc
     2941 aacgagtttg gatacatcat tagtaagtag tttttgtagc agctcaaccg ctttaggccc
     3001 tctcacccaa atttcaccca tgtgcgatac atcgaaaact ccaactgctt tgcgtactgt
     3061 ttcgtgttca gcgttaactc cttcaaattg gataggcatg ttaaaacctg caaaaggcac
     3121 gatcttagcg ccaagcgctt cgtgcatcct gataaatgct gtatttttca ttgtaaaaga
     3181 atatgaatat taattttaac tgcaaaaata ggccattaga gtagtaaata ctgaaaaaag
     3241 ggaaattatt tccgatagtc tgatttttag acttaaaagt attcggtaaa aagccccctt
     3301 tggataaatt ggattaatca agattaaaca tttgtaatca gcacaataat aattgcactg
     3361 aacaaatagt aaattttgat tggctttata aaatcaatag ttcaacctac ggttattttt
     3421 tattgattaa ttagagttta aaatcaggta cctatttaaa tttaatgtta gttttaaatc
     3481 aaaaaataat aatcgtcagg attttattca ctcagcgctt cgttaacaag cctcgcagca
     3541 tcttcaagag caattgcaga acgtacttct aatcccgatt catcaagaag tattttggcc
     3601 tcttctgcat tagtaccctg aagtcttact ataatgggca cattgatatt gtcaatatta
     3661 ttataagcat ctataattcc ttgtgctaca cgatcgcaac gaactattcc gccaaaaata
     3721 tttacaagta tcgccttcac atttggatct ttcaggatga tacggaagcc ctcttctaca
     3781 cgttttgcat tggcagtgcc accgacatcg aggaagttgg ccggatcgcc tcctgacatt
     3841 ttgatcatat ccatggtggc cattgccaat ccggcgccat ttaccataca tcctacgttt
     3901 ccgttaagct taacataatt cagatcataa cggcttgctt ctacttcaat aggatcttct
     3961 tcggctaagt cgcgcatttg tgcaatatca ggatggcgat aaagtgcatt attgtcaatt
     4021 gttactttcg catctacagc gaatatttta tcatccgaag cttttaatac aggattaatt
     4081 tcaaacagtg tagcatcact atctaaataa gctttgtata gcgattgtac aaacttaacc
     4141 atattcttaa atgcatctcc ttcgagacta agattaaatg caaccctccg agcttgaaat
     4201 ccttgtaaac catagcgtgg gtcaatctct tcggtaaata tctgatctgg tgtcttttca
     4261 gctacagctt caatatccat tccacctata ggtgaataca ttatcatgtt tctgcctgtt
     4321 tttctgttaa gcagcacact tatgtaaaac tcccttatat cagacggacc gggataaaaa
     4381 acgtcctgct gaattaaaac tttactaacc tttttgcctt ccggtggagt ttgtggggta
     4441 atcaggttca tgccaattat cttatcagta aatttttcaa cctccctgac atttttggct
     4501 aattcactcc accccctttt cctcgtccgc cagcatgtat ctgggctttt acaacccaaa
     4561 acttagtacc ggtaatagcg tgtaaatgct tggcagcctc aacagcctgc tcaggagact
     4621 cagccaagat gccttcggga acggaaaccc catatttttt taaaattgat ttgccttgaa
     4681 actcatgaag atccattgtt gtagctaatt attatatctg aatattcttt tgaaaacaaa
     4741 tacaaaaata ttatttttca agtcaatcta agccatgtag atagaatgaa aataaaaagc
     4801 caaaaatacc tgacaagaat tatcaaaaag aaacacaaaa aaaacagttc aaacatttga
     4861 ctataaatct aatcaatctt acagaacagt ttaccgatga gttatcttgt aaatttaaat
     4921 tcaaagctat gcatgaaaaa ttagaagtta cttgccttca ttgtggaagt aaagaccatt
     4981 attagcaatc aaatttttgg atgtttcaat aaaaaatgct aaaaaaagta acaactttac
     5041 gcaacggtac aggctaaggc gaaaggtaac tatggaataa ttgcaaatat taaaaggaga
     5101 cttttataaa gatcaccgaa aattttgaaa tatttttaaa tatgagtagc aaattaagga
     5161 taatatgttt gccttaaatc aattaacctt aattcattat tgataaaatc ttaatttaag
     5221 atttcataat ttttagatat ttgcactctt ttgagtcaat tgtctaacaa tttgaatcta
     5281 attatgaaat ttcttttttt cttgccggaa tgatatcatg aaaagctgtt tagtctcttt
     5341 ttttgttttt atttttttct ttttccaaca caatcccttt tctcttaatg cacaagaggc
     5401 ttcagggctt gagtcgaatc aatatatagt tcgtactgct gaaaggccac ctattgatct
     5461 tcttcaaatt ccggtagagg cttacgaagc tggaaaattt aaaattaaat tgaagccaga
     5521 gcgggaaaat ttgattcctg tactatcatc atccacaagt cctgttggct acgtggttac
     5581 ggggattcct tcttttgatg ccctcaatca atattattcc ataaaaagtt atcgtccttt
     5641 gtttggtcat ctttatcata cccgatcaga tagcgatcag tttatggaac gacacaaagc
     5701 atgggggttt catctttggc ttgaagtaat cgttgatgat aaaacggaca ttattgatgc
     5761 tgtgatgcag ttttcagccc tgagcgaagt tgattttgcc gaacccgaat accggaaaag
     5821 gcttgttatc aatgatgcaa agttcaatag tgcaaatctt gtaatttcaa atgaaaatga
     5881 gcctgagggg cgttggacgc ctaatgatcc acaatatggc aatcagtggc attataaaaa
     5941 cacaggacaa caaggaggga caccgggcgc agatatcagc ttgcaagaag catggaatat
     6001 tgaaaaggga aacagtaatg ttatagtagc tgtaattgat ggcggtatct catatacaca
     6061 tcaggatctt gcttccaata tgtggagcga aataggttac aattttgtta acggaagccc
     6121 tacagttact cctcatgatc atgggacaca cgtagcgggt actatagccg ccgttacatc
     6181 caatatgatt ggtgttgctg gtgtagcagg aggttctggt actggagacg gagttaggtt
     6241 gatgtcttgt caggtatttt ctaataatgg tagcggcggg tttcatcttg ctccgatata
     6301 tgctgccgat aatggagctt cgatctctca aaacagttgg ggttattcaa atgcaggcgt
     6361 ttatgatcaa tcggtattag atgctattga ttatttcaac gtttatggcg gaggagaagc
     6421 aataaacggt ggaatcactc tcttttcagc aggtaatagc aattccccag gacaatggta
     6481 tcccgcatgc tattcaggat gtttttctgt agctgccact aataatcagg ataaaaaagc
     6541 ttggtattcc aattatgata catgggtaga tatctctgca cctggtggtg agactaacag
     6601 tgtaactgca aggggtgtac ttagtaccct gcccaacaat agctatggtt attttcaggg
     6661 aacatcaatg gcttgccctc atgtatcagg ggttgtagct ctgatgatct cgttaggcta
     6721 tggagaactg acttcattac aaatagatag tattttacgt aataccactg ataaccatta
     6781 taatataaac tcaaattatc ttggaaaact tggcagtggg cgattaaatg cacattcagc
     6841 tctattagca gttcaagagc ttgttcaaca agtcaacaac cctcaaagtt ttaaagcatc
     6901 ggctattaat gcaaatagaa taaaccttac ttgggtaaaa aatacaagta ataacaacgt
     6961 gatggtcgtt tggtctttaa ataacgtttt tggaacacca atagatggta atgtctataa
     7021 tgtggggact acgttacctg gtggtggaaa agttttatat cgcggaagta atactaattt
     7081 ttcgcacctt aaccttcaac ctctcacaac atactattac aaagccttct cttttaattc
     7141 agccaatgag tattcaactg gcattacggc cagcgccact actcttatgc agccttatgc
     7201 tgatttttat gctaatccga caactcaagt agtttcatta ccaattactt ttaccgattc
     7261 atcaaatgga gcaactttta gctcatggtt ttgggatttt ggcgaaggcg caaatcctgc
     7321 caccactact ggcaaggggc cacatcaagt agtttataat acaccaggta gtaagacggt
     7381 tactttggtt gtggataata attacacaaa gattaaagaa aattatatta atattctatc
     7441 tgattgttac tcaataagtg aaacctattc taatggtgac atccaaaccg atttaaattt
     7501 tcaaaccctt cccggctcat catcttgccc agggtcatta attgtatcta tcccattggg
     7561 agctgtcatt ttaggagttg atgtaagtta taatatgact gcccgtaata atggttggaa
     7621 atcagagcaa agatcacaac tgaaatgtgt ttctcctggt ggattgtcag aaccagagtt
     7681 gtattccggt tccggaaatt caacaggcac tttaagctat aacagaaacg gacttaatat
     7741 tgccaatggt gttgaaggcg gtggagatat tcatttccag ttgcatgcag gcagaacctt
     7801 tggtgggtca ggttgtaata cagtttacaa taaagttgat aataatactt ggaatattac
     7861 agtatattat tatctccttg aacttgaaaa ccctccaata gcagatttca ccgctacgcc
     7921 aaccacagtt tacgccggag aaactgttag cttta
//
LOCUS       DAOOAI010000097         7933 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_11019_length_7933_cov_4.503046, whole genome shotgun sequence.
ACCESSION   DAOOAI010000097 DAOOAI010000000
VERSION     DAOOAI010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7933)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7933)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7933
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11019_length_7933_cov_4.503046"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /locus_tag="PK345_07245"
     CDS             complement(<1..586)
                     /locus_tag="PK345_07245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34847.1"
                     /translation="MRKLLILTLGLFLFIGAYSQPNMRTTAFNHLRYGKLDKAKEAID
                     KAIEHPKTINDERTWFFYGNIYLAIHLSDEAAYKELDANALDKAYNAYKKALEFDAKG
                     SNTEEINERLVVCAEQYFNRGVGFYNESKFAEASSAFENAGMINAGVGITDTISYFYT
                     AQSAYFSDQYDISKNYFGKLVSMNYKEPAIYRFLA"
     gene            complement(687..3257)
                     /gene="gyrA"
                     /locus_tag="PK345_07250"
     CDS             complement(687..3257)
                     /gene="gyrA"
                     /locus_tag="PK345_07250"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HPX34848.1"
                     /translation="MENNFEEGRIVKVNIENQMKSAYIDYSMSVIVSRALPDVRDGLK
                     PVHRRILFGMLDLGILSNRSYKKSARIVGEVLGKYHPHGDTSVYDAMVRMAQPWSMRY
                     PLIDGQGNFGSIDNDSPAAMRYTEARLRKLSEEMLEDINKDTVDFQLNFDDSLEEPTV
                     LPAKVPNLLINGVSGIAVGMATNMAPHHLGETINGCIAYIDNPDITVAELMEHIKAPD
                     FPTGGIIYGYDGVREAYETGRGRIIIRGVATIESFAGKEQIIVTSVPYQVNKAEMIRK
                     TADLINDKKIEGIVDIRDESDRNGIRIVYDLKRDAVSNVVLNKLYQLTALQTSFSINN
                     IALVRGRPVQLNLKDIVRNFIEHRHEIVTRRIRFELKEAEKRAHILEGLLIAMDNLDE
                     VIAMIRASQTPEEARDGLQTRFGLTELQARAILDMRLQRLVGLEREKIKSEYQELLEK
                     IEYYNKVLESVEMRMEIIKNELIEIREKYSDERRTTIEYATSDFRIEDTIPDDNVVVT
                     ISHLGYVKRTQLTEYRVQGRGGRGAIGGTARDEDFLEHLFIATNHNYLLLFTEKGKCY
                     WLRVFEIPEGSRTSKGRAIQNLINIEPDDKIRAFINTKDLKDEEYVANNFIVMATTKG
                     MIKKTSLEAYSRPRQNGINAIIIREDDTLFEARLTNGNNEIIMAGRSGRAIRFNEKNV
                     RAMGRNSSGVRGMKLHSDKDEVIGMVCLEDQECDVLVVSENGYGKRSKLEDYRVTNRG
                     GKGVKTLNITQKTGDLIAIKNVKDNDDLMIITKSGLTIRMAVSELRVMGRATQGVRLI
                     NLKDDDGIAAVAKVEQDENDENDENDENIDITVEEDDDYSSKENNESIDKNNKDTDTE
                     "
     gene            3601..6177
                     /locus_tag="PK345_07255"
     CDS             3601..6177
                     /locus_tag="PK345_07255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019538965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HPX34849.1"
                     /translation="MEAKFSQRIKDVLTFSREEALRMGNEYIGVEHLMLGMLREGRGR
                     AIQILNFLGIDLFEFRKSIEKSVNTEVKSYARKPENLPLVKQAERALKLTYLEAKLFN
                     STLIGTEHLLLAILKDEDNLVTNTLSKLGIDYETVKNELQAMKSNEIGKENKPLDETK
                     SKETTAQFSGDPPEEEFDEGGIKYSKKTVASKSKTPVLDNFGRDLTRAAEEGRLDPIV
                     GRERELERISQILSRRKKNNPILIGEPGVGKSAIAEGLASRIVARKVSRALFNKRVVT
                     LDLASIVAGTKYRGQFEERMKALLNELEKNPDIVLFIDEIHTLVGAGNASGSLDASNM
                     FKPALARGEIQCIGATTLDEYRQYIEKDGALERRFQKILVDPTTVDETITILQNIKEK
                     YEDHHLVKYTDDALKSCVTLTNRYISDRYLPDKAIDALDEAGARVHITNMHVPSRIIQ
                     LENKIEEVKANKTKAIGSQKFEEAANFRDIERKLQNELEEEKKKWEAESRTNRETVSE
                     ENVAEVVAMMTGIPVKRIAQSEGKRLINMDEELKSSVIGQDEAIKKVVKSIRRNRAGI
                     KDPNKPIGSFIFLGPTGVGKTYLAKVLARFLFDSEDALVRIDMSEYMEKFSISRLVGA
                     PPGYVGYEEGGQLTEKVRRKPYSIVLLDEIEKAHPDVFHLLLQVLDDGMITDSLGRRI
                     DFKNTIIIMTSNIGSRQVKEFGQGVGFNTAARESEKDKFSQSIIENALKRAFAPEFLN
                     RIDDIVIFESLKIDDIHKIIDIELKALFKRISEMGYHLTITDNAKAFIVEKGWDEKFG
                     ARPLKRAIQKYIEDPLAEEIISAKLAEGDQITLDFKEGETELDISIIKPKIKQEPEVL
                     SN"
     gene            complement(6364..7104)
                     /locus_tag="PK345_07260"
     CDS             complement(6364..7104)
                     /locus_tag="PK345_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34850.1"
                     /translation="MGFPKILLISFGALVLIVIVLIWFYPVFHIIFSKWVFGKFSARY
                     IETYKKYTGKSPYGYCIKDDLVNYLASFFQNKSNYSTYKSSKPINFYNTPFGTKFEKV
                     LSDNRKPFCVNSNRTEQFEFKVVGYRSEMFDIDMKSYFYFLNEKFFMGEYVFKNPSNE
                     KLKELAVIIKKKYIPEQSLDSYDFLIMGINDSKLRFEHTGFNLSIKYMDFSNQDVIDQ
                     LENYWSLSVKTWVKQPESDFESELFHKL"
     gene            complement(7341..7412)
                     /locus_tag="PK345_07265"
     tRNA            complement(7341..7412)
                     /locus_tag="PK345_07265"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7378..7380),aa:Met,seq:cat)
     gene            7504..>7933
                     /locus_tag="PK345_07270"
     CDS             7504..>7933
                     /locus_tag="PK345_07270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosome-binding GTPase"
                     /protein_id="HPX34851.1"
                     /translation="MKHKAGFVSILGKPNVGKSTLMNALIGERLAVITNKAQTTRHRI
                     KGILSGDDFQIVFSDTPGILKPYYKMHEAMMTQVDEAIEDADIVIYMIDPETRELDAT
                     LQQLTKKKLHKLIIAINKIDTCSQAETESLLLHWQQMLPQA"
BASE COUNT         2527 a         1529 c         1407 g         2470 t
ORIGIN      
        1 ctgctaagaa acgatagata gcgggttctt tataattcat cgaaactaat tttccaaaat
       61 aattttttga aatatcgtac tgatcagaga aataagcgct ttgagcagta taaaagtacg
      121 atatggtatc ggttattcca acaccggcat taatcattcc agcattttcg aatgccgatg
      181 aggcttctgc aaatttcgat tcgttataaa agccaactcc tcgattaaaa tactgctctg
      241 cacaaacaac taatctctca ttaatctcct ctgtattact tccttttgca tcaaattcca
      301 aagctttttt ataagcatta taggctttgt cgagagcgtt ggcatctaat tctttatatg
      361 cagcctcatc cgacagatgg atagccagat aaatatttcc atagaaaaac catgtacgct
      421 catcattgat ggtttttgga tgttcgatgg ctttatcaat ggcctctttg gccttgtcaa
      481 gttttccgta cctcagatga ttaaaagctg tggtacgcat gttgggttgc gagtaagcac
      541 ctataaacag gaataatcct aaagttaaaa tcaataattt tctcatgaca ataattattt
      601 ttaattaaac aatttaatca aatgttacac caaacaatag tatgtcaaac tttttagcat
      661 aaatagagtc tgatattata tttaaactat tcagtatcag tatctttatt atttttatca
      721 atggattcat tattttcttt tgaagaataa tcatcatctt cttccacagt gatatcaatg
      781 ttttcgtcat tttcatcatt ctcatcattt tcatcctgct ctacttttgc cacggcagcg
      841 ataccatcat catcttttaa atttataagt cgaactcctt gcgttgctct tcccatcacc
      901 cgcagttctg aaacagccat tcttattgta agccctgatt tagtaatgat cattaaatca
      961 tcattatcct ttacattttt tattgcaata agatcgccgg ttttttgcgt aatatttagt
     1021 gtttttactc cttttccacc tctgttggta actctataat cttctaattt agagcgcttt
     1081 ccatacccat tctccgaaac cactaaaaca tcgcactctt gatcttccaa acacaccatt
     1141 cctattactt catccttatc cgaatgtagc ttcattcctc gaacgccgct actatttcta
     1201 cccatggctc gcacattctt ttcattaaac ctaatagcac gacctgatct gccagccatt
     1261 ataatttcat tatttccgtt agttaacctt gcttcgaaca atgtatcatc ctcacgtatt
     1321 ataatagcat ttatcccatt ttgcctcggg cgtgaatagg cctctaacga ggtctttttt
     1381 atcatacctt ttgtagttgc cataactatg aaattgttgg caacatattc ctcatctttt
     1441 aggtctttgg tattgatgaa agctcgtatc ttatcgtcag gttcaatatt tatcaagttt
     1501 tgtattgctc tacctttgga cgttctgctt ccttccggaa tttcaaacac cctcaaccag
     1561 tagcattttc ctttttcggt aaaaagcagc aggtagttat gatttgttgc aataaaaaga
     1621 tgctctaaaa agtcttcatc tcttgccgtt ccaccaattg ctcctctccc accccttcct
     1681 tgtacgcgat attcggtcaa ttgagttctc ttaacatagc ctaaatgaga gatagtaaca
     1741 acaacattgt cgtcgggaat ggtatcttca attctaaaat cgcttgtagc atattcgatt
     1801 gtggttctac gttcatcact atatttttct cttatctcaa ttaattcatt tttaatgatt
     1861 tccatccgca tttcgacact ttcaagcacc ttattataat actcaatttt ttccaacaat
     1921 tcctgatatt ccgatttgat tttttcacgt tcaaggccaa cgagccgctg aagcctcata
     1981 tcgagtatag cgcgggcttg gagttcagta agcccaaacc gtgtttgcaa tccatctctg
     2041 gcttcttctg gtgtttggga tgcccttatc attgctatca cctcatcaag attatccata
     2101 gcaataagta acccctcaag aatatgggca cgtttttcag cttcttttaa ctcaaaacga
     2161 atacgacggg taacaatctc atggcgatgt tcaataaaat ttcgtacaat atcttttaaa
     2221 tttaattgaa ccggtcttcc tctaacaagt gcgatattgt tgatgctaaa agaagtttgt
     2281 aaagctgtta attggtataa cttattgagc accacattac ttaccgcatc tcttttaaga
     2341 tcataaacaa tgcgtattcc attccggtcg gactcatctc taatatccac gatcccctct
     2401 attttcttat cgttaatcaa gtcagccgtt ttccttatca tttcggcttt attcacctga
     2461 tacggtacag aggttacgat gatttgttct tttccagcaa aagattcaat tgtagcaacg
     2521 cctcgtatga ttattcgtcc tctacctgtt tcataggcct ctcttacacc atcataacca
     2581 taaattatcc cgccagtagg gaaatcgggt gcttttatat gttccataag ttcggcaaca
     2641 gtaatatcag ggttgtcaat atatgcaatg caaccattta ttgtttcgcc caaatgatgg
     2701 ggcgccatat tagtagccat cccaacggca ataccggata cgccatttat gagaagatta
     2761 ggaactttag ccggtaaaac tgtaggttcc tctaaagaat catcaaaatt taattggaaa
     2821 tcaacggtat ccttattaat atcctccagc atttcctccg acaatttcct gagtcgggct
     2881 tctgtataac gcattgctgc agggctatcg ttatcaatag agccaaaatt accttgtccg
     2941 tcaataagtg gataacgcat actccacggt tgagccattc ttaccatggc atcgtaaact
     3001 gaagtatcgc catgcggatg atactttccg agcacctcgc caacaatcct ggcagatttc
     3061 ttataagacc ggttggaaag aataccaaga tcaagcatac cgaacagaat ccgcctgtgc
     3121 acaggcttca aaccatctct aacatcaggt aaagcccttg aaacaattac tgacattgaa
     3181 taatcaatgt aagcggactt catctgattt tcaatattta ccttaacaat ccttccctct
     3241 tcaaaattat tttccattaa ctaaccaatt caaaattaaa tagatatctt taaaagatat
     3301 ttgctgcaaa agtactaaat tatatcggtt tattgtttta tatcgtaaca tttgttttta
     3361 acactttttt gttgataatt tttcaaggtg gttttcacag taaaattttg tcatacaaaa
     3421 cgaacttttt tctttctgaa catttgaata gcaatttttg ttatgttaca taggttttaa
     3481 tcattgatta acacaagctt ttataaagat tttagaaaac taaattataa aaaattgttt
     3541 tttttgtatc tttggaatat catttgatca cctaaggctt ttaaaccgaa aacatttata
     3601 atggaagcaa aattttcgca acgaatcaaa gacgtactga catttagtcg tgaagaagcg
     3661 ttacgaatgg gcaatgagta tattggagtt gaacatctga tgcttgggat gttaagagag
     3721 ggacgtggtc gagcaataca gattttgaat tttcttggta ttgatctttt tgagtttcgc
     3781 aaatctattg aaaaatcagt caatacagag gtgaaatcat acgcaagaaa acctgaaaac
     3841 ctcccgttag ttaaacaagc tgaacgagca ttgaaactaa cttatttaga ggccaagtta
     3901 tttaacagca cacttatcgg cactgaacat ttgcttcttg ccattttgaa agatgaagat
     3961 aatttggtta caaacaccct gagtaaactt ggtatagatt atgaaacagt aaaaaacgaa
     4021 cttcaagcaa tgaaatcgaa cgaaatagga aaagagaata aaccactgga tgaaaccaaa
     4081 agcaaagaga ctactgcaca attttcgggt gatcctccag aagaggagtt tgatgagggc
     4141 ggtattaagt attcaaaaaa aacggttgca tcaaaatcaa aaactcctgt tcttgacaat
     4201 tttggacgtg atctaacacg tgctgctgag gagggacgcc ttgatccaat tgtagggcgt
     4261 gaacgtgagt tagaaaggat ttctcaaatt cttagtcgcc gcaaaaaaaa caacccaatt
     4321 cttattggcg aacctggtgt tggaaaatcg gctattgccg aaggacttgc atcaagaatt
     4381 gttgcaagaa aagtatcgcg tgcccttttc aacaaacgtg ttgtaacact tgaccttgca
     4441 tcaattgttg caggaacaaa atatcgtggg caatttgaag agagaatgaa agctctgctc
     4501 aatgaattgg aaaaaaatcc cgacatcgtt ctttttattg atgaaataca cacacttgtc
     4561 ggtgcaggca acgcctccgg ctcgttggat gcatccaaca tgtttaaacc agcgttggct
     4621 cgtggagaga tacaatgcat cggggctaca acattagacg aatatagaca gtatattgag
     4681 aaagatggag ctctcgaacg tcgttttcag aaaatattgg ttgatcccac taccgtagat
     4741 gaaacaatta cgatacttca aaacattaaa gagaagtatg aagatcatca tcttgtaaaa
     4801 tatactgatg atgctttgaa gtcatgtgta acgctgacta atcgttacat cagcgatcgt
     4861 tatctgcccg acaaggcaat tgatgcgttg gacgaagcag gtgcgagagt tcatattacc
     4921 aacatgcatg ttccctctcg tatcattcag ctagaaaaca aaatagaaga ggtaaaggct
     4981 aacaaaacaa aagccattgg tagtcagaaa ttcgaagagg ctgcaaactt tcgggacata
     5041 gaaagaaaac ttcaaaacga acttgaagag gaaaaaaaga aatgggaagc cgaatcgcgt
     5101 acaaaccgcg aaacagtttc cgaagaaaac gtggcagagg tagttgcaat gatgaccggc
     5161 atccctgtca aacggattgc acaaagcgag ggcaagcgtc tgattaatat ggacgaagag
     5221 ttgaagagtt ctgttattgg gcaggatgaa gcaattaaaa aagttgtaaa atcaattcgt
     5281 cgaaaccgtg cagggataaa ggacccgaac aagccgattg gaagttttat ttttttaggg
     5341 ccaacaggtg ttggaaaaac ctatttagcc aaagtccttg ctcgattcct cttcgattcg
     5401 gaggatgccc ttgttagaat tgatatgagt gaatacatgg agaagttctc catttcaaga
     5461 cttgttggag ctcctccggg atatgttggt tacgaagagg gaggccagtt aacggaaaaa
     5521 gtaaggcgca agccttattc tatcgttttg ctggacgaaa tagaaaaagc gcatcccgat
     5581 gtctttcacc ttctattgca agtacttgat gatggcatga tcactgatag tcttggcaga
     5641 aggatagatt ttaagaacac cattataatt atgacctcca acattggcag ccgccaagta
     5701 aaggaattcg gacagggagt tgggttcaac actgctgcac gcgagtcaga aaaggataaa
     5761 ttttcgcaaa gtattatcga aaatgcttta aaacgggcat ttgcacctga atttctgaac
     5821 agaattgacg acatagttat tttcgagtct cttaaaattg atgatattca taagattata
     5881 gacatcgaac ttaaagcgct ctttaaaaga ataagtgaaa tgggttatca tcttacgatt
     5941 actgacaatg ctaaagcttt tattgtggaa aagggctggg atgaaaaatt tggagccaga
     6001 ccgctaaagc gagctattca aaaatacata gaagatccgt tggctgaaga gataatcagt
     6061 gcaaaactcg cagaaggcga tcagataact ctggatttta aggagggaga aacagaactt
     6121 gatatttcga taatcaaacc caagataaaa caagagccag aagtgctgtc aaactaatcc
     6181 cgaattaggg tttaatgaat acaattctta ttgagttgat tacaaatagt aaatccttta
     6241 agcatagtca agtcaattgc ttcatttgga attacttcaa atttagattg gctgaaatag
     6301 acaaaaagag agtacaataa aaatttactc tctatttcga ttttataata aaacgttgtc
     6361 agtctataac ttatggaata attcagattc aaaatcggat tcaggttgtt ttacccatgt
     6421 ttttactgat aggctccaat agttttcaag ttgatcaatc acatcctgat tcgaaaaatc
     6481 catgtattta atggaaaggt taaacccagt atgttcaaac ctaagtttgg agtcgtttat
     6541 acccataatt aaaaagtcgt aagagtcgag tgattgctcc ggaatatatt tctttttaat
     6601 aataaccgct aactctttaa gcttttcatt gcttgggttt ttaaaaacgt actctcccat
     6661 aaaaaacttt tcgttgagaa aataaaagta cgatttcatg tcaatatcaa acatctcgct
     6721 acggtagcca acgaccttaa attcaaactg ttcggttctg ttagagttta cacaaaatgg
     6781 cttgcggtta tcggaaagca ctttttcaaa ctttgttcca aaaggggtat tataaaaatt
     6841 aatcggcttt gaacttttgt aagtactgta attactttta ttttgaaaaa agctcgccaa
     6901 ataatttact aaatcatctt taatgcaata tccataaggg ctttttccag tatatttctt
     6961 gtaggtttca atgtaccttg cactgaattt cccgaacacc cactttgaaa agatgatgtg
     7021 aaaaactgga taaaaccata ttaagacaat aacaattaaa acaagcgctc caaagctgat
     7081 aagaagaatt ttaggaaatc ccatttcaaa aaaaattagg cgacaaattt ataagaattc
     7141 attttcgatt gacttaacaa aaaacaataa gttatgttaa ccgatagaaa atcctgaagt
     7201 tttatcttta tggacatgat ggccgtaagg tttcacaact taaaacctcc tgttttcatc
     7261 ttttaacgat aagttccaac agtaacaaaa aaaaaccgct gttagacggt cagtttaaca
     7321 gcgggctaat ttttaagttg tagcggggga aggattcgaa cctgcggcct ttgggttatg
     7381 agcccaacga gctacctctg ctccaccccg cactattttt tttaaaacga cacctacatt
     7441 ggtaaccttt tcgggctgca aagatataat ttgtttcttt gtaaatcaaa tttttgtaaa
     7501 aaaatgaaac ataaagcggg atttgtcagt attcttggaa agccaaacgt tggaaagtca
     7561 acattgatga atgctttaat aggtgaaagg cttgcagtaa ttacaaataa agcgcaaact
     7621 acccggcaca gaattaaagg cattttgagt ggcgatgatt ttcaaattgt cttttccgat
     7681 actcccggca ttttaaaacc ttattataaa atgcatgaag cgatgatgac gcaggttgat
     7741 gaggcaatag aggatgccga tattgtaatc tacatgattg atcccgaaac ccgtgaactt
     7801 gatgctacct tacaacaact taccaaaaaa aagttacaca agctgattat tgcaatcaat
     7861 aagattgata cttgttcgca ggctgaaaca gagtcgctgt tattacattg gcaacagatg
     7921 ctgccacaag ctg
//
LOCUS       DAOOAI010000098         7767 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_11306_length_7767_cov_5.514782, whole genome shotgun sequence.
ACCESSION   DAOOAI010000098 DAOOAI010000000
VERSION     DAOOAI010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7767)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7767)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7767
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11306_length_7767_cov_5.514782"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1002)
                     /locus_tag="PK345_07275"
     CDS             complement(<1..1002)
                     /locus_tag="PK345_07275"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34852.1"
                     /translation="MTTRRFKMLDAILFVILLLIGYGCSVKNKFSIINFAHLYNHAEK
                     PLITGLKIFHESDSNSRVYVRYKPSSLHYVLKMGRSYYRADYSISYRLFSSYESAAIV
                     DSSTFKMFDSLYFQNDLSLVYNFPIKTLPDNNYVVEIKFSDSNRGIDSYYSQEINRKD
                     ANNSQHFLLVDQQDEVIFEEWVSWKTKFRIITPKTNLTKLFVDYYDDVFPAARTPFSL
                     SHSPVYQLKPKEKYILEINDGITDYLQYGREGFFHFRSDSMTKDGFTIFRFHDDYPEI
                     KESELMPGPMRYITTDPEYAMLAASKEPQTIVEKFWIEVAGNERRGFQLLENYYDLVK
                     "
     gene            1125..1949
                     /locus_tag="PK345_07280"
     CDS             1125..1949
                     /locus_tag="PK345_07280"
                     /inference="COORDINATES: protein motif:HMM:NF015506.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0031992 - energy transducer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy transducer TonB"
                     /protein_id="HPX34853.1"
                     /translation="MKTGNIFHKLFFNGLVLLSALYVNAQYYEYKPIENFGGKSQLHE
                     FLSSEMVYPAKALSEKVEGRVVIHFGVNKMGEVTYSDIQQSVSDDLDAEALRLFRYFL
                     WEPAAYRSTPVDDEAVMEVDFKIKKYQRNVKKRGYDQIIYPYSLVDSSFTIFKPAQLT
                     RPPRPIFEKQGMRFADFIQQNMVYPSVALKQSISGMVEMFFVVEPTGRVSNIKLLKSV
                     SGGCNEEAVRLLKMLSWAPGIHGEKAVRTEMIMQIRFNLADFQEMRYIPPSNNNQF"
     gene            1955..2464
                     /locus_tag="PK345_07285"
     CDS             1955..2464
                     /locus_tag="PK345_07285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018709531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HPX34854.1"
                     /translation="MKKIGLLSDTHGTWPKEAPNFFSPCDEVWHVGDIGNILLLDEIE
                     SYKPLRAVYGNIDNHEIRLRFPEINVFTIEQVKVVMMHIGGYPGHYEKKARAAITTHR
                     PNLFISGHSHILRIIFDSFYNLLHINPGAAGNFGFHTLVTLVRFDINGDKIENLEIFE
                     KDRAGRKEI"
     gene            complement(2534..3349)
                     /locus_tag="PK345_07290"
     CDS             complement(2534..3349)
                     /locus_tag="PK345_07290"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: protein motif:HMM:NF006054.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HPX34855.1"
                     /translation="MKGETKQTIDYLHSLGIDKPEIAIILGTGLGGLVDFIEIEKIVN
                     YGDIPHFPVSTVEFHKGKLIYGNLCNKKVLVMQGRFHYYEGYNMKQITFPIQIMKALK
                     VKWLFVSNAGGTVNPDFKKGDLMLIVDHINLFWDSPLIGRNDDSIGPRFVDMSDAYSS
                     ELNDLFLKVAAQEQITLHRGIYTAVCGSSLETRAEYRYLRFIGTDVVGMSTIPEVIAC
                     KHANLPVAAISVITDECDPDNLEPIDIDDILKTAADAEVNLITLFKNVLIKMR"
     gene            3473..4318
                     /locus_tag="PK345_07295"
     CDS             3473..4318
                     /locus_tag="PK345_07295"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HPX34856.1"
                     /translation="METIISLKAMGSQVRRDILRMVHGAKSGHPGGSLGCVELLVALY
                     FDIMNHDPDTFEMSGLGQDVFLLSNGHISPAWYSVLARSGYFDIAELATFRKLNSRLQ
                     GHPATHEGLPGVRFASGSLGQGLSCAIGVALGKQLDEDENLVFVLMGDGEVQEGQVWE
                     AAMFAGSKNVDNLIAIIDFNRKQIDGSTDDVMPLGDLKSKWETFGWKVLEMKGNDLEE
                     VIDVLRLATLETGKDQPVMILMHTEMGFGVDFMMGTHKWHGVAPNDEQLQLALAQLNE
                     IIGDY"
     gene            4329..5303
                     /locus_tag="PK345_07300"
     CDS             4329..5303
                     /locus_tag="PK345_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase family protein"
                     /protein_id="HPX34857.1"
                     /translation="MENLSNLNWENIGSKDTRSGFGDAIYELGKEREDVVALCADLTG
                     SLKLEKFADEFPNRFFQIGIAEANMMGIAAGLATTGKVPFTGTFANFATGRVFDQIRQ
                     SIAYSEKNVKICASHAGITLGEDGASHQIMEDIALMKTLPGMTVINTCDYNQTFAATK
                     AIAEWEGPVYLRFGRPKVPNFTPKDYKFKIGEALLLKEGSDVSIFATGHMVWKALEAA
                     HALVEQGISAEVINIATIKPLDRNAIIESVLKTRCAVTAEEHQIIGGMGESIAGFLAR
                     NFPTPIEMVGMNDVFGESGTPEELMEKFGMGTKDIIEAVSLVIARKQF"
     gene            5380..6495
                     /locus_tag="PK345_07305"
     CDS             5380..6495
                     /locus_tag="PK345_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HPX34858.1"
                     /translation="MANYFYLRNDFPKISDREYVVERLIQLKEQLRKTVPMKEVENTL
                     IIGTWNIRDFGKVNRRGYGKRTKDSHYYIAEIISAFDFVAVQEINELEEWNKVMRILG
                     PSWDFIATDITHYKLGGNEERLTFVYDKRKVTFKNIAGEIVLPNDMLISDSEITNSPD
                     PMADGKQFRRTPFIVSFQSGWLKFDICTVHIYYGQGTSGLKQRIQEIKAIGKYLSTRA
                     DKALKKEGKATILLGDFNIIKPNHKTMDALEENGFVIPKNIKSRKTNVIETMHYDQIA
                     FKSDKELIDFVDTNSNSGVFKVFGSVFKDTDEDFQFYKSEVLKSSKAAKIKDDDVALK
                     EYYNKEWKTYQMSDHNLLWARVSNDKSEKYLRSLTDK"
     gene            6896..>7767
                     /locus_tag="PK345_07310"
     CDS             6896..>7767
                     /locus_tag="PK345_07310"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34859.1"
                     /translation="MTKTTFHNIPSLISKVFGNNIELLPSVDELFELELAYLEFNSLS
                     IEQLIGRSAFIKSIDNQSTKHYLLYSNYNEVINENRSAQTQTYFEEGQFSTGYATHGL
                     FPYRGKFHPQLIKALLNILGVEKGETLLDPMAGSGTANVEAALMGINSIACDVSPFCQ
                     FMIKVKYESLKIDLNLLKNTPFNNKKLFDFFKQGNVLWRIGKVKDIEKQKIYNLVFLA
                     WLDALGYSKRVVRSNHERLFEKVLPRYIETVKSTLKNPYFNQNEIGKVEVLSDSEALN
                     IKLPKESIDCIITSP"
BASE COUNT         2555 a         1322 c         1573 g         2317 t
ORIGIN      
        1 ttttacaaga tcataataat tttcgagtag ttgaaagcca cgcctttcgt tcccagccac
       61 ctcaatccag aatttttcca caatcgtttg cggctctttt gatgctgcca acatcgcata
      121 ttcaggatcg gtagttatat aacgcatcgg gccgggcatc agctcgctct cttttatttc
      181 aggataatcg tcgtgaaatc taaaaattgt gaagccatcc ttggtcatac tatcgcttct
      241 gaaatgaaaa aaaccttcac ggccatattg caaataatct gttataccat cattaatttc
      301 taaaatgtac ttctccttcg gctttaattg ataaacaggt gaatgcgata gtgaaaaagg
      361 agttcgtgcg gcaggaaaaa catcatcgta gtaatctaca aataacttgg tgaggttggt
      421 ttttggagtt atgatacgaa attttgtttt ccaactgacc cactcctcga aaatcacctc
      481 atcttgttga tcaactaata ggaaatgttg actattattg gcatcttttc gatttatttc
      541 ttgagaataa tagctgtcaa tccctctgtt actatcgcta aatttaatct caacaacata
      601 attattatca ggcaatgttt ttattggaaa gttgtaaacg agtgacaaat cattctggaa
      661 gtatagcgaa tcgaacattt taaatgtgct gctatcaact attgccgcag attcataact
      721 actaaataat ctgtagctaa tcgaataatc agctcgataa tatgaacgtc ccatttttaa
      781 cacgtaatga agacttgatg gcttgtagcg aacataaact ctcgaattgc tgtcggactc
      841 atggaatatt tttaagccag ttatcaaagg cttttcagcg tggttataaa ggtgagcaaa
      901 attgattatc gaaaatttgt tttttaccga acaaccgtag ccgattaaga gtaagatgac
      961 aaaaagtatt gcatccaaca ttttgaagcg tcgagtagtc atggttcttt atttaatttg
     1021 gcagtaaagg taataacaat cttggatttt tcggatttga tgaaaaatgt cctttagtat
     1081 taataaattt aatacattta cactttcata aaaatgccgg aattatgaaa acggggaaca
     1141 tttttcataa actgtttttt aatggactgg ttttattatc agccctttac gttaatgccc
     1201 agtattatga atataagccg atcgagaatt ttggagggaa aagtcagttg cacgaatttt
     1261 tgtcgtcaga aatggtctat cctgctaaag ctctttctga aaaagttgaa ggtagagtag
     1321 taattcattt tggagtgaac aaaatgggtg aggtaactta ttctgatatt caacaatctg
     1381 tcagtgatga tcttgatgcg gaggcattgc gtttgttccg ctactttttg tgggagccgg
     1441 ccgcctatcg gtcaacgcca gtggatgatg aagctgtgat ggaagttgac ttcaagataa
     1501 aaaaatatca acgtaatgta aaaaagcgtg gttatgatca gattatctat ccctatagcc
     1561 ttgttgatag ttcatttacg attttcaaac ctgcacaact caccaggccg cctcgcccga
     1621 ttttcgaaaa acaagggatg aggtttgcgg atttcattca gcaaaacatg gtttatccct
     1681 ctgtagcgct aaagcagagt atctcaggta tggtagagat gttttttgtg gttgaaccga
     1741 caggaagagt ttccaatatc aagttgctga aaagtgttag cggtggatgt aatgaggagg
     1801 ctgtaagact gcttaaaatg ctttcctggg caccaggtat ccatggcgaa aaggccgttc
     1861 gtaccgaaat gatcatgcaa ataagattta atcttgccga ctttcaggag atgcgatata
     1921 tcccgccaag taataacaac cagttttgat tagaatgaaa aagattggtt tgttatccga
     1981 cacacatggg acatggccaa aggaggctcc caattttttt agcccatgcg atgaggtttg
     2041 gcatgtaggc gatatcggca atatattgct tttagatgaa atagagagct acaaacccct
     2101 cagggctgtt tacggaaata ttgataatca tgaaatacga cttagatttc ctgaaattaa
     2161 tgtttttacc attgaacagg ttaaagttgt gatgatgcac atcggaggct atcccggtca
     2221 ttacgaaaaa aaagcaagag cagccataac cactcatcgc cccaatctat tcatctccgg
     2281 tcattcacat attctgagga ttatttttga ttctttttat aatcttttac atataaatcc
     2341 tggagctgcc ggaaatttcg gattccatac attggtaact ttagtcaggt ttgatataaa
     2401 tggagataaa attgaaaatc ttgagatttt tgaaaaagat agagctggta gaaaagagat
     2461 ataattattt gatttgcaaa tttttataag taaagatgta atcacattaa attatcgctt
     2521 ttagcgccag ggtttaccgc attttgatca atacattttt aaatagtgta ataagattta
     2581 cctctgcatc agccgctgtt ttcagtatgt cgtcaatatc aatcggttcc aaattatcag
     2641 gatcgcattc atcggtaatc actgatattg cagcaacagg taggtttgca tgcttacatg
     2701 ctatcacttc aggaatagtt gacatgccca caacgtcagt tccaataaat cgtaaataac
     2761 ggtattcggc gcgggtttcg agcgatgagc cgcaaacggc agtataaata ccccgatgca
     2821 aagtaatttg ttcttgagca gcaaccttca gaaacaaatc gttaagctcc gacgaataag
     2881 catcactcat atcaacaaat cgaggtccta tactgtcgtc atttctgcca attaaagggc
     2941 tgtcccaaaa cagattaatg tgatccacaa tcaacatcaa atcacctttt ttgaagtcag
     3001 ggtttacagt tcctccggca tttgaaacaa agagccattt aacctttaat gctttcataa
     3061 tctgtatagg aaaggttatc tgtttcatat tgtaaccttc gtaataatga aatctgccct
     3121 gcatgactaa tactttttta ttgcaaagat taccatagat caatttccct ttatggaact
     3181 ctaccgttga aaccgggaaa tgagggatat cgccataatt aactatcttt tcaatctcga
     3241 taaaatcaac caatcccccg agccctgttc cgagtattat ggctatttcc ggtttatcta
     3301 ttcctaagct gtgcaaatag tcaatagtct gtttggtttc gcctttcata atctcttttc
     3361 gttttattaa tcttttgttg gcaaaataat acaataaaga catttgcata aaaaaaatgt
     3421 gataaagaaa ctaaaaaaga tatttttgta gaatatatga aatgtcaaaa taatggaaac
     3481 tattatctcc ttaaaggcta tgggcagtca ggtaaggcgt gatatcttaa ggatggttca
     3541 cggcgctaaa tcaggtcatc cgggcggttc gcttggttgt gttgaactat tagttgcgct
     3601 ttacttcgat attatgaatc atgatccgga cacttttgaa atgagtgggt tagggcaaga
     3661 tgtctttttg ctatcaaacg gacacatttc tccggcatgg tatagcgttt tggccagaag
     3721 cggctatttc gacattgccg aactggctac tttccgtaag ctaaactctc gtctgcaagg
     3781 tcatccggct acccatgaag gattgccggg tgtgcgattt gcctccggat cgctggggca
     3841 aggactctct tgtgctattg gggtggcgtt gggcaagcag ctggatgagg atgagaattt
     3901 agtttttgtt cttatgggag atggcgaagt tcaggaagga caagtgtggg aagcagccat
     3961 gtttgccgga agtaaaaatg tggataacct catagctatt attgatttca atcgcaaaca
     4021 gattgatgga tcaaccgatg acgtcatgcc gcttggtgat ctgaagagta aatgggaaac
     4081 ttttggctgg aaagtgcttg aaatgaaagg taatgacctg gaagaggtta ttgatgtttt
     4141 gaggcttgca acattagaga caggtaaaga tcagccggta atgatactta tgcatactga
     4201 aatggggttt ggagtggatt ttatgatggg aacacacaaa tggcatggag tagcccctaa
     4261 cgatgaacag cttcaattag ccttagcaca actcaacgaa ataattggtg actattaaat
     4321 ttcgaaaaat ggaaaattta tcaaatttga attgggaaaa cataggtagt aaagataccc
     4381 gttccggttt tggcgatgcg atttatgaac ttggcaaaga gagagaagat gttgtagctc
     4441 tttgcgccga tttaacagga tcattgaaat tggaaaagtt tgccgatgag tttccgaatc
     4501 ggttttttca gattggaatt gccgaagcta atatgatggg aattgctgca gggcttgcta
     4561 ctactggtaa ggtacctttt acaggaactt ttgcaaactt tgcaacaggt cgagtttttg
     4621 atcaaatcag acaatctatt gcctattccg aaaaaaatgt gaagatttgt gcctcccatg
     4681 ctggtattac ccttggcgag gatggggctt cgcatcagat tatggaggac attgcgctga
     4741 tgaaaaccct gcctggtatg acagttatca atacctgcga ttataatcag acatttgctg
     4801 ccactaaggc aatagcggag tgggaggggc ctgtatatct acgatttggt cgccctaagg
     4861 tccctaattt cacacctaaa gattataagt ttaaaatagg agaggccctg ttgttaaagg
     4921 agggtagcga tgtgtcaata tttgctaccg gacacatggt atggaaagcg cttgaagcag
     4981 cacatgcact ggttgaacaa ggcattagcg ccgaagtaat aaatatcgct actattaagc
     5041 cactcgatag aaatgcaatt attgagtctg ttcttaaaac acgctgtgct gttactgccg
     5101 aggaacatca aatcatagga ggaatggggg agagtattgc agggtttctg gctcgaaatt
     5161 tcccaacacc tatagagatg gtgggaatga atgatgtttt tggcgaaagt ggaacgccag
     5221 aagaactaat ggaaaaattt ggtatgggaa caaaagatat tatagaggcc gtttcacttg
     5281 ttattgccag aaaacagttt taaaaaaaca aattaatgat attttaaatc aataagttat
     5341 agaatgcttt tgaaatatta aacctaaaaa aagaatacca tggcaaatta cttttatctt
     5401 agaaatgact tcccaaaaat cagcgacagg gaatatgttg ttgaaagatt gattcaactg
     5461 aaagagcagt tgagaaaaac cgttccaatg aaagaggtcg aaaacacttt gatcattggt
     5521 acatggaaca taagagactt tggtaaggta aatcgtagag gttatggcaa acgtacaaaa
     5581 gactcacact attatatagc ggaaattatt tctgcatttg actttgttgc cgttcaggaa
     5641 atcaatgagc tggaagagtg gaataaggtg atgcgaattt taggtccttc ctgggacttt
     5701 attgcaacgg atatcactca ctataagctt ggcgggaatg aagaaaggct gacatttgtg
     5761 tatgacaaac gaaaagtaac ctttaaaaat attgccggcg aaattgtgtt accaaacgat
     5821 atgctaatca gtgattctga gataacaaat tcgccagatc caatggctga cggcaaacag
     5881 ttcagaagaa ctccctttat cgtttcgttt cagtcgggct ggctgaaatt tgatatttgt
     5941 actgtacata tttattatgg acaaggcact tccggtttaa aacagagaat tcaggagatt
     6001 aaagccatag gaaaatacct aagcacccgg gctgacaaag ccctcaaaaa agaaggtaaa
     6061 gcaacaatac tcttaggcga ttttaatatt atcaagccta accataagac aatggatgca
     6121 ttggaagaga acggttttgt gataccaaaa aatataaaat cccgaaaaac aaatgttatc
     6181 gaaaccatgc actatgatca gatagctttt aaatcggata aagagcttat tgactttgtt
     6241 gacacaaact caaattcagg agtttttaaa gtgtttggat cagtttttaa agatacagat
     6301 gaagattttc aattttataa atccgaagtt cttaaatcga gtaaagcagc caaaattaaa
     6361 gatgacgacg ttgcactgaa agaatattat aataaggaat ggaaaactta tcagatgtcc
     6421 gatcataatc tgttgtgggc aagagtgagt aacgacaaaa gcgaaaagta tttaagaagc
     6481 cttacggata aataagcgca tctttatgat aacaatccgc ctcaaaaaga gagattggct
     6541 ttataaaatg atacatataa acgagttagc tatcatttac ctgcaacact atacccctaa
     6601 gaaaatagct ccaataattt tgttgagaac taaccaaaaa aatcaacgtt taattcgcat
     6661 aaaagttata ttttgcaaaa atcttcaagg aataaagata ttgaaatatt atgaaattta
     6721 tagaacgaat aaaataatta agaaaagagc cccaactgcc gcaacgaaaa ttggcgacta
     6781 ctttagatat agatacggct acttattgta aaataaagaa aggtgagcga cgagctgaaa
     6841 attatactga tcgcattgtt tgacaaaaac actaacaaat aaaataattt gggtgatgac
     6901 aaaaacaaca tttcacaaca taccctcatt aataagtaaa gtctttggta acaatatcga
     6961 acttttacca agtgtagatg aattatttga attagaattg gcttatttag aatttaatag
     7021 tctttcaata gagcaactca tagggcgttc tgcatttata aaatctattg ataatcaatc
     7081 tacaaaacat tatttattgt attcaaacta caatgaggta ataaacgaaa ataggtcggc
     7141 acagactcaa acttattttg aagaggggca attttcaaca ggttacgcca cgcacgggct
     7201 tttcccatat cgtggcaaat tccacccgca actgattaaa gcattattaa acattttggg
     7261 agttgaaaaa ggcgaaacac ttttagaccc aatggcaggt agtgggacag ctaacgtcga
     7321 ggctgcttta atgggtatta attcaatagc ttgtgatgtt agtccatttt gtcagtttat
     7381 gataaaagtc aaatatgaat cgcttaaaat tgatttgaat ttattgaaaa acacgccatt
     7441 taataataaa aaactatttg atttttttaa gcagggcaat gttttatggc gaataggaaa
     7501 ggttaaagat atagaaaaac aaaaaatcta taacctcgta tttttagcat ggttagacgc
     7561 tttaggatac tcaaaacgag ttgtacgttc taaccacgaa cggttgtttg aaaaagtttt
     7621 accacgttat atagaaacag taaaatcaac attaaaaaac ccgtatttta atcaaaacga
     7681 aatcggaaaa gttgaagtct tgtccgattc agaagcatta aacattaaac tgccaaaaga
     7741 aagcattgat tgcataatta cttcacc
//
LOCUS       DAOOAI010000099         7658 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_11501_length_7658_cov_5.264369, whole genome shotgun sequence.
ACCESSION   DAOOAI010000099 DAOOAI010000000
VERSION     DAOOAI010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7658)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7658)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7658
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11501_length_7658_cov_5.264369"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1651
                     /gene="porU"
                     /locus_tag="PK345_07315"
     CDS             <1..1651
                     /gene="porU"
                     /locus_tag="PK345_07315"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HPX34860.1"
                     /translation="EQLAVYVDTTYKNYNVDKIYIDAYFQESTTGGARYPSVNKAINT
                     RIEKGTLIINYTGHGGELGWAHERILENADINSWKNYNKLAVFITATCEFARYDDPGR
                     ISAGEYVFLNPSGGGIALFTTSRLTYGSHNLSLIAGIYKYAFEQNVQEFYSMGDLLHI
                     AKLESPDYLNDRKYILLGDPALKIAHPYYNIETTHINQRPINENNEPDTLKALSTVTV
                     SGRITDEFNNAVSDFNGVLNITVFDKETEVTTLGQDPGSYPKSFMLRKNTLFNGRTEV
                     INGTFTFSFIVPKDIAYQYGFGKISYYADDGDNDASGFYDNIVVGGYDTTAKMDNKGP
                     EIKLYMNDTTFVNGGITNENPILLALVFDESGINTVGNSIGHDITAILDGNSDNPFLL
                     NDFYQADLRGFQSGRVRYPFYNLTPGKHEIKFKIWDVFNNSSESFIEFEVISQYNMVI
                     EGVRNYPNPVTDYTYFVFNHNKADSELEITLDIFDLSGRNVVTLKQFDTSTSFTINPI
                     YWDGTNGNGMFLRKGIYIYRIIAKDNAGKIASGSNKLVILK"
     gene            1683..5501
                     /gene="porU"
                     /locus_tag="PK345_07320"
     CDS             1683..5501
                     /gene="porU"
                     /locus_tag="PK345_07320"
                     /inference="COORDINATES: protein motif:HMM:NF033707.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system sortase PorU"
                     /protein_id="HPX34861.1"
                     /translation="MTKKLLIFTILYLSTLSFLIAQPSVLDETIQWSDFQQFKMNDAS
                     LKVLYFTDAINAPEFGLLPVYSRLFRIEKKETAYSFNIINTVYQEFDNQLELQDVEDI
                     HLIPEQLTYSSSIAIIRNRHYAELTILPLRKNIETGLIEKLISFQIEIVTTYDQNATM
                     MNKSALFTNNSVLSEGDWFKISVSKNGIYKLTYSDLTAMGMNLSGVNPDHIRIFGNGG
                     GMLPEKNSDARRDDLQEIAIAIYDGNDGNFDEGDYILFYAEGPDKWSYVPIYLAFKFD
                     RHKYSDYNYYFVTVKEGQGKRIIPQKQVLTLPIHEVTSYNYYETFDLDSINLIKSGSE
                     WYSHEFFDVTHRTYTFDFPYRKVNENVFISIDVAARSFVESFFDIYANEELLFNLSLP
                     KVTPTSTRYASEFDGTRRLIIPGNQSISFDVVYNKPTQESYGWLNYIDVNVINRLEYH
                     GSQMSFRNVMAMYLDTIARFKMTNVNSQVTIWDVTDHQDVRSINFNMSGDTCSFVSET
                     NLLREFIAFDHTEFYSVEILGKISNQNLHALGEADFIIVSHSDFFEQAERLKALHETI
                     DNMTVHLVDPQNIYNEFSSGKPDPSAIRDFVRMIYQKSPETPRLKYLLLFGDGSYDPK
                     NRIENNKNFILTFQSKQSLIFTTSYVTDDFYGLMDENEGADAAGNLDIGIGRFPVNTL
                     LEAKQLVDKYERYMTLSPDNEGQWRNDFCFIADDEDNNLHIINADTVLVKLVRERNKT
                     ININKIYFDAYHQEKTSAGDRFPDATIAINEQMNRGALFVNYIGHGGEIALAHERVVQ
                     IQDILSWNNHSKLPVMITATCAFTPYDNPSLVSAGENVILNPNGGAIALMSTTRVAFA
                     SYNLTINMRIYDTLFSASTGQYPRVGDLIMCSKVPNNVHFRNFTLFGNPALKIAFPES
                     KIIIDSINGKPATIYRDTLKAGDHVNFSGYIADHDINTIHSNFNGTLFPVLYDKAKKI
                     STLANDPASMVYQFDLQQEIIYKGMAEVKNGRFLFSFVIPQDIMYNYGAGKLSLYASD
                     GQHDAAGYFNNLILGGYLENIEDQSGPDIELYLNSKTFYDGCTTHNSPTLYVDLYDES
                     GINHLGIGIGHDIVANLDGPVNDSFILNDYYVPQVNDFRSGSIVFPLNNLPNGQYNLR
                     LKAWDVVNNSSVATISFIISDNIKTNITQVFNYPNPVREYTYFTFQHNQFDSPLTIKI
                     NIHDFTGKLVHTIGPIEIVGSGYNIEPIYWNGEANNTKLKSGLYVYRIEVTSALGGTS
                     VINQKLIISN"
     gene            5580..6773
                     /gene="porV"
                     /locus_tag="PK345_07325"
     CDS             5580..6773
                     /gene="porV"
                     /locus_tag="PK345_07325"
                     /inference="COORDINATES: protein motif:HMM:NF033710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IX secretion system outer membrane channel
                     protein PorV"
                     /protein_id="HPX34862.1"
                     /translation="MINRSFFLLLFFLLTVHVIQGQNKPNYLGQTNTITTAVPFLLIA
                     PDSRAGGMGDAGVATTPDASSMHWNPAKYAFIDSKAGFAATYSPWLRAIVSDINLAYV
                     AGFYKIDDRQTIASSLLFFSLGDITFTDIRGDFIGNYRPNELAIDATYSRKFSDKWSG
                     AVAARYINSNLTQGINVSGVATKPGRSIAADVSVYHQQELNWRNVEYAEFAFGLNISN
                     IGSKISYSDATTDTDFIPTNLRFGPRLSIDLDEYNRLSFFADVNKLLVPTPPIYALDE
                     NGQTITDENGNKKIEAGKDPNVNTIQGLLQSWYDAPGGFSEEMREFYYSIGTEYWYNK
                     AFAVRAGYFWEHENKGNRKYFTLGAGLKYNVFGLDFSYLIPTQQRNPLENTLRFTLSF
                     DFDDF"
     gene            6858..7340
                     /gene="ispF"
                     /locus_tag="PK345_07330"
     CDS             6858..7340
                     /gene="ispF"
                     /locus_tag="PK345_07330"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HPX34863.1"
                     /translation="MQIRIGVGYDVHRLEPGMPFMLGGIKIPYSKGAIGHSDADVLIH
                     AICDALLGAANLRDIGFHFPNNDPSYKGIDSALLLEKVTELLKQKGYSIGNIDATIVL
                     EKPKLATFIPQMISRLTQIMEIGEERVSVKATTTEKLGFEGREEGVTAHAVALIIKIE
                     "
BASE COUNT         2460 a         1447 c         1473 g         2278 t
ORIGIN      
        1 tgagcaactt gcggtttacg tggatacaac atataaaaat tataacgtag ataaaattta
       61 tattgatgcc tattttcagg aatcaactac tggaggcgct cgctatccgt cagttaataa
      121 agctataaac actcgaatag aaaaaggaac tttgattatt aactatactg gccatggtgg
      181 ggagcttggc tgggcacacg agcgtatcct cgaaaacgct gatatcaaca gttggaaaaa
      241 ctataacaaa ttagccgttt ttattacagc aacctgcgaa tttgctagat atgacgaccc
      301 tgggagaatt tcagcaggag agtacgtttt cttaaatcct tcaggtggag gaatagccct
      361 atttactaca tcaagactta cttatggatc gcataatcta agcttaatcg caggcattta
      421 taaatacgct tttgaacaaa acgtacaaga gttttattca atgggcgact tattacatat
      481 tgcaaagttg gagagccctg actatcttaa cgatagaaaa tatatccttc tcggcgatcc
      541 ggcgctgaaa atagcccatc cttactataa catcgaaact acacacatta accaacgccc
      601 tatcaatgaa aataatgagc cagatactct aaaagcgctc tcgacagtta ctgtttcggg
      661 tagaataact gacgaattta ataatgcagt atctgatttt aatggagttt taaatatcac
      721 cgtttttgat aaagagactg aggtaaccac attgggacaa gacccgggaa gttaccccaa
      781 atcctttatg ttacgcaaga ataccctttt taatggaaga accgaagtga taaatggaac
      841 atttaccttt tcatttatag taccgaagga tattgcatat caatacggat ttggaaagat
      901 tagctattat gcagatgatg gggataacga tgcttcagga ttttatgata atattgttgt
      961 cggcggctac gatactactg ccaagatgga taacaaagga ccggaaatta agctatacat
     1021 gaatgataca acttttgtaa acgggggtat aacgaatgaa aaccctatct tgcttgcact
     1081 ggtgtttgat gaaagtggaa taaataccgt agggaatagt ataggccatg atattacagc
     1141 aatattagac ggaaattcag ataacccttt cttattaaat gatttttatc aggctgattt
     1201 acgtggattc caaagcggaa gagttagata tccattttat aaccttactc ctggaaaaca
     1261 cgaaataaaa ttcaagatat gggatgtgtt caacaactct tcagaaagtt ttattgagtt
     1321 tgaggtgata agtcaatata atatggttat cgagggtgtg agaaactatc cgaatccggt
     1381 tactgactat acatattttg tttttaacca caacaaagct gatagcgaat tagagatcac
     1441 cttagatatt tttgatctat ccggaaggaa tgttgttacg ttgaagcaat ttgataccag
     1501 tacctcgttt accatcaatc ctatctattg ggatggaacc aatggaaatg gtatgttttt
     1561 acgaaaaggg atctatattt atcgcatcat tgctaaagat aatgccggta aaattgcttc
     1621 aggatccaac aaattggtta ttctcaaatg atatttgaaa caataagtct aactaaataa
     1681 taatgactaa aaagcttttg atatttacga tattgtatct atccactctc tcatttttaa
     1741 ttgcacagcc ttcggtttta gatgagacaa ttcaatggag cgactttcaa cagtttaaga
     1801 tgaatgacgc atctttgaag gtgttatatt ttactgatgc tattaatgca cccgaatttg
     1861 gactattacc ggtttatagc cggctttttc gtatcgaaaa aaaagaaact gcatattctt
     1921 tcaatatcat taacactgta tatcaggaat ttgataatca gcttgaacta caagatgtcg
     1981 aagatattca tttgattcct gagcagctta catactcatc gtcaatagct ataattcgta
     2041 atcgccacta tgctgaactg acaatcctac ccttgcgaaa aaatattgag acaggcttaa
     2101 tcgaaaaact tatctctttt caaatagaga ttgtaaccac atatgatcaa aatgccacta
     2161 tgatgaacaa gtctgctctt tttactaaca attctgttct ttcagaaggt gattggttta
     2221 aaatctcagt ttcaaaaaat ggtatttata aattaaccta ctccgacctt acagcaatgg
     2281 ggatgaattt atccggtgta aaccccgatc atattcgtat ttttggtaat ggcggaggca
     2341 tgttgcctga aaagaatagc gatgctcgac gtgatgattt acaagaaata gctatagcga
     2401 tttacgatgg caatgatggt aactttgatg aaggagacta cattttattt tatgccgaag
     2461 gccccgacaa atggagttat gttcctattt acctcgcctt taaatttgat agacataagt
     2521 attctgacta caattattat ttcgtaaccg taaaggaggg acaaggaaaa agaatcatcc
     2581 ctcagaaaca ggttttaacc ttacctatcc atgaggtaac cagttataat tattacgaaa
     2641 cattcgacct tgacagtatt aatttgatca agagcggttc agaatggtat agccatgagt
     2701 tctttgatgt tacccaccga acttatactt tcgattttcc ttatcgaaag gtcaatgaaa
     2761 atgtatttat ctccattgac gtagctgcca gatcgtttgt agaatcattt tttgatattt
     2821 atgcaaacga ggaactgctt tttaatcttt ccttgccgaa ggtaacccct acaagcacta
     2881 gatatgccag cgagtttgac ggtacccgcc gacttatcat tcccggcaat caatccatca
     2941 gctttgatgt tgtttacaat aagccaactc aggagagtta tggatggctt aattatattg
     3001 atgtaaacgt aatcaaccgg ttagagtatc atgggagcca aatgtcattt cgaaatgtga
     3061 tggcaatgta tctcgatacc attgcgcggt ttaaaatgac caatgtaaat agccaggtaa
     3121 caatctggga tgttaccgat catcaagatg tacgatctat caatttcaac atgagtggag
     3181 atacttgttc atttgtttca gagactaatt tgttacgcga attcatcgct ttcgatcata
     3241 ccgaatttta ttctgttgaa atccttggga aaataagcaa tcaaaacctt catgcgcttg
     3301 gtgaggctga ttttatcata gttagccact ctgacttttt tgaacaggct gaaaggctaa
     3361 aagcccttca cgaaacgatt gataatatga ctgtacactt ggttgatcct caaaatatct
     3421 ataatgagtt ttcttccggg aagcccgatc cctccgcaat tcgtgatttt gttagaatga
     3481 tctatcaaaa atcgccagag acaccccgat tgaagtattt gttgcttttc ggagatgggt
     3541 cttacgatcc gaaaaataga attgagaaca acaaaaattt tattctaacc ttccagtcga
     3601 agcaatcact catttttaca acctcctatg ttacagatga cttttacggg cttatggatg
     3661 aaaacgaagg ggctgatgct gcaggaaatc tcgatatcgg aattggcaga ttccctgtga
     3721 atacactact tgaagccaag cagcttgttg ataaatatga gaggtacatg actttgtcgc
     3781 ccgacaatga aggccagtgg cgaaatgact tctgtttcat tgccgacgac gaagacaata
     3841 accttcatat cataaatgct gatacagtac ttgttaagct tgttagggag agaaacaaga
     3901 cgataaatat caataagatt tattttgatg cataccatca ggagaaaaca tcagcgggag
     3961 acagatttcc ggatgctacc attgccatta atgaacagat gaacaggggc gccttatttg
     4021 taaattatat cggccatggc ggcgaaattg ccctggcaca cgagcgtgta gtacagattc
     4081 aggatatttt atcatggaac aaccatagca aattacccgt tatgataacc gctacctgcg
     4141 cgtttacccc ttatgataac cctagtctgg tatctgctgg cgaaaacgta attctaaatc
     4201 ctaatggcgg agcaattgcg ttaatgtcaa caacaagagt tgcatttgca tcatataacc
     4261 tgacgatcaa catgcgtatt tacgatacgt tatttagtgc atcaacaggg caatacccac
     4321 gtgtaggcga tctaataatg tgttcaaaag tccctaacaa tgttcacttc cgaaatttca
     4381 ccctgtttgg aaatcctgct cttaagattg ccttccctga gagtaaaatt attattgatt
     4441 ccataaacgg aaagcctgca accatatacc gcgatacttt aaaggcagga gatcacgtaa
     4501 actttagcgg atatattgca gatcatgata ttaacaccat ccacagtaac tttaacggta
     4561 cattatttcc ggtactatac gataaagcaa agaaaataag taccctggcc aatgatccgg
     4621 caagtatggt atatcagttc gatctccagc aggaaatcat ttataaaggg atggccgagg
     4681 taaaaaatgg acgatttctg ttttcatttg tcatcccgca agatatcatg tataattatg
     4741 gagcagggaa gctatcattg tatgcctctg atggccaaca tgatgcagca ggctatttta
     4801 ataatttaat tcttggagga tatctggaaa atattgaaga tcaatccggg ccggatatag
     4861 agctatacct taattccaaa actttttatg acggatgcac cacacataac agcccaactt
     4921 tatacgttga cctttatgac gaatccggaa taaatcatct tggaatcggc atcggccatg
     4981 atatagtagc aaatcttgat ggccctgtaa atgatagctt tattcttaat gattattatg
     5041 tgccacaagt caacgatttt cgttcaggct ccatcgtttt ccctttgaat aatttgccaa
     5101 atggccagta taacctgcga ctcaaagcat gggatgttgt taacaactcg tccgtagcaa
     5161 caattagttt tatcatttcg gacaatatta aaactaacat tactcaggtc tttaattatc
     5221 ctaacccagt tagagaatac acctacttca catttcaaca caatcagttc gacagccctc
     5281 tcacaatcaa aatcaacatt cacgatttca caggaaaatt ggtgcatact attggcccta
     5341 tcgaaatcgt tggtagcggg tacaatatcg aaccgatcta ctggaacgga gaagcaaata
     5401 acacgaaact taaatcgggg ctgtatgttt atcgcatcga agtaacatct gcattgggag
     5461 gtacatcagt gatcaatcaa aaactcatta tctcaaacta attgcggtaa actaaaaaca
     5521 atattaaaaa tgaatagccg tttaacatgt ttaatttcaa attggaaaaa ataatttcta
     5581 tgattaacag atcatttttt ttacttttgt tcttcctctt gactgttcac gttatccaag
     5641 gtcaaaacaa acccaattat ttgggacaga ctaataccat aactactgct gtgccttttt
     5701 tactgattgc tcctgattcg cgtgcaggcg gcatgggtga tgccggtgta gctacaacgc
     5761 ctgatgccag ttcaatgcac tggaacccgg ccaaatatgc ttttattgat tctaaagctg
     5821 gatttgcagc tacctatagc ccatggctaa gagctatcgt aagcgacata aacctggcat
     5881 acgttgctgg attttataaa attgacgata gacagactat agccagctca ttgctcttct
     5941 tctccttagg agatattact tttacagata tccgtggcga ttttatcggc aattatcgcc
     6001 ctaacgaatt agcaatagat gctacctatt caagaaagtt ttcggataaa tggtcgggcg
     6061 cagtagctgc cagatacatc aattccaatc tgacccaggg aatcaatgtc agcggtgtgg
     6121 ctacgaagcc cgggcggtca atagcagcag acgtctcagt gtatcatcag caggagctga
     6181 actggcgaaa cgttgagtat gccgaatttg cttttggcct taacatatcc aatatcggct
     6241 caaagatttc ttactccgat gctaccactg ataccgattt tatcccaacc aacttacgtt
     6301 ttgggccaag attatctatt gatttggatg aatacaacag attgtcgttt ttcgcagatg
     6361 ttaataaatt attggtgcca acaccgccga tatatgccct tgatgaaaac ggacaaacta
     6421 ttacagatga aaatggaaat aaaaaaatag aagccggtaa agatcctaac gtaaacacta
     6481 ttcaagggtt attacaatcg tggtatgatg cccctggtgg tttctcggaa gagatgcgcg
     6541 agttttatta cagcatagga acggaatatt ggtataacaa agcttttgca gtcagagccg
     6601 gatatttttg ggaacatgaa aacaaaggaa atcgcaagta tttcactctg ggcgccggct
     6661 tgaaatataa tgtcttcggg cttgatttct catacctaat acctactcaa cagcgtaacc
     6721 ccttagagaa tacacttagg tttacactct cattcgactt cgacgatttt tagggttcag
     6781 atataataaa gtgaaataat taaaccggtt agagagatct gccggttttt ttgttagttt
     6841 tggcacaaaa taatatcatg caaatacgta taggcgttgg ttatgatgta catcggcttg
     6901 agcccggaat gccttttatg ttaggaggta ttaaaatacc ttatagcaaa ggggcaatcg
     6961 gacactcaga tgccgacgtg ctgatacatg ctatctgcga tgctttgctc ggcgcagcaa
     7021 accttagaga tatcggattc cactttccga ataatgatcc aagctataaa ggtattgaca
     7081 gtgccttgtt actcgaaaaa gtaaccgaac tgcttaaaca aaaaggctat tccattggaa
     7141 acatagatgc cacaatagtg ttagaaaagc caaaactcgc aaccttcatc ccgcagatga
     7201 tcagcagatt aacccagata atggagatcg gggaagaaag agtttcagta aaagccacaa
     7261 caactgaaaa attaggcttt gaaggcagag aggagggcgt aacagcccat gcagtagcat
     7321 tgattataaa gatagaataa aagcttgcca caaatgcata gatattcgtt tttcattcgt
     7381 gaatttgtgg cttagattat ttttaaggag cggacacaat cgttaaaacg aaaaatgaaa
     7441 ttgtgtaatt tgtctttcta tcatgttatt aggcatttag aatagacaaa gatggcaatt
     7501 tgaaatgaaa ctgaaccata aaatactatt tcgtgtaatg aaactgaacc acaaaacttt
     7561 attttttgta atgaatagag agttctcatt gcatttttca tttgattagc tcctcaggca
     7621 atattgtttc atcaaaggaa tcgtaataat tttgattt
//
LOCUS       DAOOAI010000100         7570 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_11643_length_7570_cov_4.330007, whole genome shotgun sequence.
ACCESSION   DAOOAI010000100 DAOOAI010000000
VERSION     DAOOAI010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7570)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7570)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7570
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11643_length_7570_cov_4.330007"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2575)
                     /locus_tag="PK345_07335"
     CDS             complement(<1..2575)
                     /locus_tag="PK345_07335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HPX34864.1"
                     /translation="MTTLNHNSDDQSKEIIQLKEELSQLQESLRLAQKHISEIEQSSE
                     NIHLAIINILPNGALGYLNPYASSLFGYKRDEDFITKLYDIILPFDDQLPVDFHIRVA
                     RLISRGDSFFRHETKILTPEKKAIWITWTVQLLYNETFDFNGLVAMGSDITSLKQSEN
                     ALRQNEFVLKKAHEVSKTGSWVWDPDTNTVKGSELFFKIFGAPPTQNYQKMMKDLKKI
                     ILPEYFDFIKTKINRALTRGTTESYTYKILHPSGNIRWIIEEVCLLGSDEENGDRIVG
                     TVRDITDQMTAEEKLRENLLIVSSSSELMSLISPDYRYINVNQAFLDAYGKTISQVEG
                     HTIAEIFGEEIFQNLMKSKIDRCMLGESISEQNWTKFTDGSKRYMDAKFNPVREIDGM
                     ISGVTVSTRDITDLITTQEQLRIFRLFAEESGVGFGMANLDGKITYVNSTLCRLAGFN
                     KPNDLIGKSIYETYLKKYYNLVKNEIIPTIKHEGHWTGEIEMLRHDGEEAYTIQNFFL
                     IRNEKEEPVSYALSVTNITKRKIAEQALLQSESNFRTIFNNAAIGINVIDKSGKFLQV
                     NHAMVDMLGYTENELLKMKIEDITFPEDIVLSINLLRALFDGKNEHYRLDKRYIRKDG
                     STFWADVLVTLFFDIVKQKRYAIETIIDITESKTLQQQLEQSREEAIRANMAKSEFLA
                     NMSHEIRTPLNAVIGFTELLENLVTDKKKISYLDSIKSGGKNLLLLINDILDLSKIEV
                     GKYNIKFEPVNPFSLINEIRQIFSLKIKEKNLDFIVDVDPGIPSSLELDEIRIRQVLF
                     NLIGNALKFTESGYVKFSVKKLADSVSNNKIDLEFSVEDTGIGIPEDQHHVIFDAFQQ
                     QF"
     gene            2938..4881
                     /locus_tag="PK345_07340"
     CDS             2938..4881
                     /locus_tag="PK345_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HPX34865.1"
                     /translation="MAYIKFDKTQLINLEYSLNRELLRSNRAGAYSSTTIVNCNTRKY
                     HGLLIIPQPQFGNDPHVLLSMLHETVIQHDASFNLGIHKYQNGVYSPKGHKYVRNFEL
                     DPTPKITYRVGGVVLEKEQLFSSHENQIIIKYTLVDAHSETKLQFKPFLAFRNIHQLS
                     KANHDANKRYESVPNGIKIVMYEGYSPLYLQFSKKPEYTHVPDWYYNIKYTHEELRGY
                     DPLEDLYVPGFFEVQIKKGESIIVVASTTEVNPSGMKQRFLAEAKKRRPRNSFENCLI
                     NSAQQFVVKQNKKVDIIAGYPWYGSIGRDTFMALPGLLLTQGETNTFIEVINSMIANM
                     KGAFFHNAILNHKITYDSVDTQLWFFWALQKYVEKTGEKTKIWTDYNRIIKLILNSYR
                     EGTDYNIKMQQNGLLFAGQSGVALTWMNAYIDGKPVTPRIGMPVEVNALWYNAIMFSL
                     DLAKAAKDTIFSKQWKSVAESIKKAFVKEFWSNEKGHLADYINDSFKDWSVRPNQLLA
                     VSMPYSPLDDLQQKKILDIIDRELLTPKGLRTLSPMHLKYKGVYRGNAQERDEAYHQG
                     SVVSWFSGHYAEAYLKVYKTGGFQVIKNLYKGFEPDIQNYGIGTMPEIYDGDPPHNPA
                     GATSMAVAVAELLRINEMLNKFE"
     gene            4916..6196
                     /locus_tag="PK345_07345"
     CDS             4916..6196
                     /locus_tag="PK345_07345"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HPX34866.1"
                     /translation="MKVLMFGWEFPPHISGGLGTACYGLTKGLANHNVETIFVVPKAY
                     GDEDQSAIRLVNASDIIVDSTEEVYQEFWKKITYLEIGSNLIPYVSPQEFARIAQESQ
                     FEGSSLEKKVTAAKFEFTGKYGTDLMAEVSRYALVAAGIAAKMDFDVIHAHDWLTYSA
                     GITAKKMSGKPLVVHMHATEFDRSGVNINPNVYDIERRGMEEADRVITVSNLTRATVI
                     EKYGINPDKVYTVHNAVEPTDKEVEEVKRHVKEKVVTFLGRLTFQKGPEYFIEAASII
                     LQHDPNVRFVMAGSGDLMNKMIRRVAKLKIGTRFHFTGFLKGADVDKMFLLSDVFVMP
                     SVSEPFGIVPLEAMRSNVPVVISKQSGVAEILKHALKVDFWDVHGLADSIYALLHYKS
                     LRETFQKYGKDEVDSLKWDNAAYKVTQVYQTLFK"
     gene            6233..7549
                     /locus_tag="PK345_07350"
     CDS             6233..7549
                     /locus_tag="PK345_07350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 57 protein"
                     /protein_id="HPX34867.1"
                     /translation="MKSVCFYFQVHQPFRLKTYRFFNMGHDHYYYNDFENSHIMSRIA
                     QKSYLPMNDLLLKLIKKYGKDFKVTFSITGHALDQFEAYAPEVITSFKKLADTGNVEF
                     LAETYAHSLASLWSKSDFYKQVERHSDKIESLFGIRPQTFRNTELIYSDEIGEMVYEM
                     GYNLMLTEGAKHVLGWKSPNFMYCNARNPKLKVLLRNFNLSDDIAYRFSNQDWNEWPL
                     TAEKFVEWLSALDKNEDVVNIFLDYETFGEHQWRETGIFDFMAALPAKILSTKQLKFA
                     TPKELEKELQPISAIHVPYPISWADDERDLTAWQGNEMQEEAISKLYELEPKIRYCND
                     QELLNDWCKLQNSDHFYYMCTKWFSDGDVHKYFNPYNSPYEAFINFMNVLSDFAIRVD
                     EECKMTDGERVIAKAEELGKEITQVTRKAVKNTTKKIKKAIEKKRG"
BASE COUNT         2398 a         1375 c         1474 g         2323 t
ORIGIN      
        1 caaattgttg ctggaaggcg tcaaatatta catgatgctg atcctctgga atcccaatac
       61 cagtatcctc taccgaaaat tctaagtcaa tcttgttatt gctaaccgaa tcagcaagtt
      121 tttttactga gaattttaca tagcccgatt cagtaaattt taatgcattg ccaatcaggt
      181 tgaaaaggac ttgcctgatt cttatctcgt ccaactcaag acttgaaggt atgccggggt
      241 caacatcaac aatgaaatcg agatttttct ctttgatctt taaagagaaa atttgtctta
      301 tctcattaat aagcgaaaag gggttgactg gctcaaattt gatattgtat ttcccgactt
      361 caatttttga cagatcaaga atgtcattta tgagtaacaa caggttttta cctcccgatt
      421 taatcgagtc gaggtagcta attttctttt tatcagttac gagattttca agcagttcag
      481 tgaagccgat aacggcatta agcggagttc tgatttcatg actcatattt gccaaaaact
      541 ccgatttggc catattagcc cttatagcct cttcgcgtga ttgctcaagt tgttgttgaa
      601 gcgttttaga ttcggtaata tcaatgattg tttcgatagc ataacgcttt tgtttaacaa
      661 tatcaaaaaa aagtgttacc aaaacgtctg cccaaaaggt agagccatcc ttgcggatat
      721 atcttttatc aagtcggtaa tgttcatttt ttccatcaaa aagtgctctc aataagttaa
      781 tgctcaaaac tatatcttcc ggaaaagtga tatcttcaat tttcattttt agcagttcgt
      841 tctccgtata gcctaacata tcaaccatag catggttaac ctgcaaaaac ttaccacttt
      901 tatcaattac attaatgcct attgcagcat tgttgaatat tgttctgaaa ttggattcac
      961 tttgcagcaa agcttgctcg gcaatttttc gtttagtaat attagtaaca ctcaatgcgt
     1021 aggataccgg ctcttctttt tcgttgcgaa tgaggaaaaa attctgaatg gtataggctt
     1081 cctccccatc atggcgtaac atctcaatct cgccagtcca gtgaccttca tgttttattg
     1141 tgggaataat ctcatttttt acaaggttat aatatttttt taaataggtt tcataaatac
     1201 tttttccgat aagatcgtta ggcttattaa acccagccag acggcatagc gtggaattga
     1261 cataagtaat cttaccatcc aagtttgcca tgccaaagcc tacgcccgat tcttcagcaa
     1321 acaatctaaa tatccttagc tgctcttgtg ttgtaataag atcggtaatg tcgcgcgtac
     1381 taacagttac acccgagatc atgccatcaa tctcccgtac cggattaaat tttgcatcca
     1441 tgtatcgttt gctgccatca gtaaatttag tccagttttg ttctgagata ctttcgccca
     1501 acatacacct gtctatcttg gatttcataa gattttgaaa aatctcttct ccaaaaatct
     1561 ccgctatcgt atgcccttca acctgcgata ttgttttacc ataagcatca agaaaggcct
     1621 gattaacatt aatgtagcga tagtcggggg atatgagcga catcaattcg ctcgaggaag
     1681 atacgataag cagattttca cgaagtttct cttctgccgt catctgatca gtaatatctc
     1741 tgaccgtgcc aactattctg tctccatttt cttcatcgct accaaggagg cacacctctt
     1801 cgattatcca acgtatattg cctgaggggt gtaatatttt ataggtgtac gattcggtag
     1861 taccacgggt tagcgctctg tttattttcg ttttgataaa atcaaaatat tcaggaagaa
     1921 tgattttttt gaggtctttc atcatcttct gataattttg agttggcggc gctccaaaaa
     1981 ttttgaaaaa caattccgaa cctttaacgg tatttgtgtc gggatcccac acccagctac
     2041 ctgtttttga aacctcgtga gcctttttta acacgaattc attttggcgc agcgcatttt
     2101 ccgattgttt taaactggtg atatcgctgc ccattgcaac taatccatta aaatcaaaag
     2161 tttcattgta aagcaattgg actgtccaag ttatccagat cgctttcttt tcgggggtta
     2221 aaatttttgt ttcgtgcctg aaaaaactat cgcctcggct gataagtcga gctaccctaa
     2281 tgtggaaatc aacgggcaac tgatcgtcaa aaggcaaaat aatgtcataa agtttagtaa
     2341 taaaatcttc atctctttta tatccaaaaa gcgatgatgc ataaggatta agatagccta
     2401 atgctccgtt cggcaagata tttatgattg caagatgaat attttcggat gactgttcaa
     2461 tttcggaaat atgcttttgc gcaagcctta acgactcttg caattgcgat agttcttctt
     2521 ttaattgaat aatctctttg gattgatcgt cagaattgtg atttagagtg gtcaatgtat
     2581 catattgatt ttgatgatta agaatgcgaa attataactt tcggaagata tgcttatttt
     2641 aatgattctc ccaataattt agaatgttac aatattctga ttaattcatt aattacggat
     2701 taagtttttg atagcgctta aacgattaag tgttttatta ctcagcacaa taaaaattgt
     2761 attgaataag catcaaaatt agattacatt tttaaaatca atcacttcac cccgttattc
     2821 tatttgttga tatgggttaa tattatgttt gtgggagcta ttaaggtatt aatgtgtatt
     2881 tttgcagttg ataaaaaagt ttaacattaa aaattaatac tcaagataat acgtaacatg
     2941 gcttacatta agtttgacaa aacacaacta ataaatttag aatattcttt aaatcgcgaa
     3001 cttcttcgat ctaaccgtgc tggagcttac tcctcaacaa ctattgttaa ttgtaataca
     3061 aggaagtatc atggattact tatcattccg cagccacagt ttggcaacga tcctcatgtt
     3121 cttctttcaa tgttacatga aacggtaata caacatgatg ccagctttaa ccttggtatt
     3181 cataagtatc aaaatggtgt atattcgcct aaaggtcata aatatgtaag aaattttgag
     3241 cttgatccta ctccaaaaat tacctatcgt gttggtggcg tcgttctgga aaaagagcag
     3301 ctcttttctt cacacgaaaa ccagattatt ataaaatata cgctcgttga cgcacattcg
     3361 gagacaaagc ttcagttcaa gccttttctt gcatttcgaa atattcatca gttgtcgaaa
     3421 gcaaatcatg atgccaataa gagatatgaa tcagtaccaa atggaattaa gatagtgatg
     3481 tacgaaggtt attcgccact ttacttgcag ttttcgaaaa aacctgaata cacacacgtc
     3541 cctgactggt attataacat taagtacacc cacgaagaat taagaggata tgatccttta
     3601 gaggatttat atgttcctgg tttttttgag gtgcaaataa aaaaaggaga atcaattatt
     3661 gtagttgcaa gcaccacaga agtaaatcct tcggggatga aacaaagatt tttggcggag
     3721 gctaaaaaac gaagaccgcg aaactcattt gaaaactgcc tcatcaactc ggcacagcag
     3781 tttgtggtga aacagaataa aaaagttgat attattgccg gatatccatg gtatggaagt
     3841 atcggtcggg atacttttat ggcattaccg gggttgttac ttacgcaagg cgaaaccaat
     3901 acatttattg aggttatcaa ttcaatgatt gccaacatga aaggggcgtt ttttcataat
     3961 gctatcctca accataaaat cacctacgac tctgtggata cacaactttg gttcttttgg
     4021 gctttgcaga aatatgtaga aaaaacagga gaaaaaacta agatatggac tgattataat
     4081 agaataatta agctcatact aaattcttac agagaaggta ctgactataa tattaaaatg
     4141 cagcaaaacg gattgctgtt tgccggtcaa tccggagttg ctcttacctg gatgaatgct
     4201 tacattgatg gtaaaccagt aacaccgcgc attggaatgc cggtagaggt gaatgcatta
     4261 tggtacaatg caattatgtt cagccttgat ctagcaaaag cagctaaaga cacaatattt
     4321 agcaaacagt ggaaaagtgt ggctgaaagt atcaaaaaag cttttgtcaa ggagttttgg
     4381 agtaatgaaa aaggacatct tgccgattac attaacgata gctttaaaga ttggtcggta
     4441 agacccaacc agcttcttgc cgtttccatg ccctactcgc cattggatga tttacaacaa
     4501 aagaaaatac ttgatatcat tgatcgtgag ctgctaacac caaaaggctt gaggacatta
     4561 tcacccatgc atttaaaata taagggggtt tatcgtggca atgctcaaga gcgtgatgaa
     4621 gcttatcatc aaggatcagt agtttcctgg ttttctggcc attatgccga agcatatttg
     4681 aaagtgtaca aaacaggcgg atttcaggta ataaaaaacc tttataaagg tttcgagcct
     4741 gatattcaaa attatggtat tggtacaatg cccgaaattt atgacggtga tccacctcat
     4801 aatccagccg gagctacatc aatggcagta gcagtagctg agctgttgag gataaatgaa
     4861 atgttaaata agtttgaata atcacaatta ggattaaaaa gtataattag gatacatgaa
     4921 agtattaatg tttggatggg agttccctcc acatatctcc ggaggattag gcactgcttg
     4981 ttatggttta accaaaggac tagccaatca taatgttgaa accatctttg ttgtgccgaa
     5041 agcatatggc gatgaagatc agtcggcaat aagattggta aatgccagcg atattattgt
     5101 tgactcgacc gaggaggttt atcaggaatt ttggaaaaaa attacctatc ttgaaatagg
     5161 gtctaacctt atcccttacg ttagtcctca agagtttgcg cgtattgccc aagagagtca
     5221 attcgaaggc tcctcgctag aaaaaaaagt tactgccgcc aaatttgagt ttacaggtaa
     5281 atatggtact gatcttatgg ctgaagtatc gcgttatgcg ttagtagctg ccggtattgc
     5341 tgcaaaaatg gacttcgacg tcatacatgc gcacgactgg ctcacatact cggccggaat
     5401 aacagcaaaa aagatgagcg gcaaacctct tgtagtacac atgcacgcta cggaattcga
     5461 tcgctcgggc gtcaacataa atcctaacgt ttatgatatt gaacgacgtg gcatggaaga
     5521 agccgatagg gttattactg tcagcaacct aacacgggca acagtaatag agaaatacgg
     5581 aattaacccc gataaagtat ataccgttca taatgcagta gaacccactg acaaagaggt
     5641 tgaagaagta aaacgtcacg taaaagaaaa agtggtaacc ttccttggta ggctcacttt
     5701 tcaaaaaggg ccggaatatt ttattgaagc tgcaagcata atcctccagc acgatcctaa
     5761 tgttagattt gtaatggccg gatcgggtga tttgatgaat aaaatgatac ggcgggtagc
     5821 aaagttgaaa ataggcacac gtttccattt tacaggattt cttaaaggag ctgatgtaga
     5881 taagatgttt ttacttagcg atgtctttgt tatgccttcg gtatccgaac ctttcggaat
     5941 agtgccactg gaagctatgc gctccaatgt acctgtagta atatcgaaac aatcgggagt
     6001 agccgagatt ttaaaacatg ccttgaaagt tgatttctgg gatgtacacg gcttggcgga
     6061 ttccatttat gctctcttgc attataaatc gttaagggaa acgtttcaaa aatatggtaa
     6121 agatgaagtt gatagcctca aatgggataa tgcagcttat aaagtaactc aagtttatca
     6181 aacattgttt aaataattac aaatcaatag aatacaaaca taaatttaaa atatgaaatc
     6241 agtctgtttt tattttcagg ttcatcaacc cttcagatta aagacttatc gtttttttaa
     6301 catgggccat gatcattatt attataatga ttttgaaaat agccacataa tgagccgcat
     6361 tgctcaaaaa tcttatttgc caatgaatga tctgttgtta aaactgatca aaaaatacgg
     6421 taaagatttt aaagttactt tttctatcac cggacatgca ttagatcagt ttgaagctta
     6481 tgctcccgag gtgattacca gttttaaaaa gctggctgat acgggcaacg tagaatttct
     6541 tgctgaaact tatgctcact ctctggcgtc attatggtcg aaatctgatt tttataagca
     6601 ggtggagcga catagcgata agattgaatc gctatttgga atacgacctc aaacttttcg
     6661 taatactgag cttatctatt cggatgaaat tggcgaaatg gtatatgaaa tgggctataa
     6721 tttaatgctt accgaaggag caaagcatgt gctaggctgg aaaagtccta acttcatgta
     6781 ttgcaacgca cgaaatccta aattgaaagt tctgctgcga aattttaatc ttagtgatga
     6841 tattgcttac aggttttcga accaagattg gaacgaatgg ccgctcactg cggaaaagtt
     6901 tgtagagtgg ctctccgccc ttgataaaaa tgaagatgta gtcaacattt ttcttgacta
     6961 tgaaaccttt ggcgaacacc aatggagaga aaccggcatt tttgatttta tggccgctct
     7021 gccagccaaa attttaagca ccaaacagct taaatttgct acgcctaagg agttggagaa
     7081 agagctacaa ccaatatcag ctattcatgt cccctatccc atctcctggg ccgatgatga
     7141 acgagatctc acagcatggc aaggcaacga aatgcaggaa gaggcaataa gcaagctcta
     7201 cgagttggag cctaaaatca gatattgtaa tgatcaggaa ttgttgaacg actggtgtaa
     7261 actgcagaat agcgaccatt tttactatat gtgcaccaag tggttctccg acggcgatgt
     7321 gcataagtat ttcaacccat ataattctcc ctacgaagct tttattaatt tcatgaatgt
     7381 attgtcggac tttgcaataa gagtggatga ggaatgtaaa atgaccgatg gggagagagt
     7441 aattgcaaaa gcagaagagt tggggaaaga gatcacacag gttactcgta aagcggtaaa
     7501 aaacacaacg aaaaagatta aaaaagcaat tgaaaagaaa agggggtaaa ttatgggtat
     7561 gcctaaggta
//
LOCUS       DAOOAI010000101         7543 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_11707_length_7543_cov_4.530449, whole genome shotgun sequence.
ACCESSION   DAOOAI010000101 DAOOAI010000000
VERSION     DAOOAI010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7543)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7543)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7543
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11707_length_7543_cov_4.530449"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            90..635
                     /locus_tag="PK345_07355"
     CDS             90..635
                     /locus_tag="PK345_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HPX34868.1"
                     /translation="MDKIQERISLLISTKGMTNTEFAEAIDVQPANISHVMSGRNRPS
                     LDLVMKIITRFPEVRTDWLLFGQGAMNKDYTLFDIEEIPVVRGRDKQERPFEKGVDSY
                     ETREILTQKGIEEEALSSVNTVKREEFSEKSLEPDNFNAGRHSDQKSGNLTSEVRSKK
                     VEKIILFYNDKSFGEYYPESD"
     gene            complement(815..1237)
                     /gene="ndk"
                     /locus_tag="PK345_07360"
     CDS             complement(815..1237)
                     /gene="ndk"
                     /locus_tag="PK345_07360"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002859435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0006165 - nucleoside diphosphate
                     phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HPX34869.1"
                     /translation="MKGNITLTMIKPTAMEKGYAGKIITRVLEAGFKLKAMKMLQLTK
                     SQAMEFYGIHTGKPFYDDLVKYMSSGPIIAAILEKENAVEEYRKLIGSTNPEKAEKGT
                     IRYDYGTNIQANAVHGSDSDENAIIESNFFFSHLERLK"
     gene            1364..2389
                     /locus_tag="PK345_07365"
     CDS             1364..2389
                     /locus_tag="PK345_07365"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HPX34870.1"
                     /translation="MSQNDIEKLIELFKNPKKIVISSHYNPDGDAVGSAMALYHVLKQ
                     LNHQVSVIFPNKFPLFLSWIEESERSLIFEQDGASASQVISAADIIFCVDYNSFKRLG
                     EMESLMVKSKAIKVLIDHHPDPEKEHFGFVYHSASASSTAELVYKFLKLTGLHSKINR
                     QVAESLYAGIITDTGSLSFSCNNPDTYIIIAKLIQKGLDAERAHRLIYDDFSENRMRL
                     LGYTIYQKMIVIPEYFTAIIALTIDELARFGYQQGDTEGIVNFPLSMKAINFSVMLVE
                     RTDRIRLSFRSKGSFPANIIAARYFEGGGHLNAAGGDSYLSMQETIKRLKEILPQYYD
                     QLNFEIR"
     gene            2407..2928
                     /locus_tag="PK345_07370"
     CDS             2407..2928
                     /locus_tag="PK345_07370"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPX34871.1"
                     /translation="MFKISMVILLPFLLFACHQSQPRESLNQEPANLKEELIEANKGT
                     VKTEDQHIEDFINRRGWKMQTSGTGLRYMITTRCNGKKAVNGDIAVLEYKTRLITGEV
                     IYSSDNDGLKEFEVGHGGVEAGLEEAVCMMQIGDKGYFILPSYIAWGLSGDGQLIPPR
                     STLIYELKLIDLK"
     gene            2945..3811
                     /locus_tag="PK345_07375"
     CDS             2945..3811
                     /locus_tag="PK345_07375"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPX34872.1"
                     /translation="MVKKSFFIITTLLLAVILISACGRSSDGFKTTSSGLKYKFHVKG
                     EKDTLNVNLYDIVYVRMDYRVADTMLFQSGVTPISFQVKPGDVGDLQEGLLMMKVGDS
                     ATFAMPAEKFFINLMKYKEVPAVFSDYAELYVDIKVVALIPEPQALKDERAAAEVRKA
                     TEQDVIKKYIEEQNITVQPTANGLYYIEIVKGKGKKAEAGNRLKVHYKGLLFNGEVFD
                     SSYDRNKPVTFTLGNGERLPAWDEGFALMAAGGKARFIVPSALGYGAEQRSKVKPYTP
                     LIFEVELLEVTD"
     gene            3862..4740
                     /locus_tag="PK345_07380"
     CDS             3862..4740
                     /locus_tag="PK345_07380"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HPX34873.1"
                     /translation="MKAKFTMFFTLLVIMGTFMISCKTRKADYKFNENGIGYLFHTKN
                     SNAQMPENGDILSMKLAYGAPDSVYFHTDSIPEKKMILPMVTSEHAGDLYEALAMMHL
                     GDSVSFLLPAETFFLKTAHFPEIPFFAKGVDKLLFNIKLDKIQTEEQMYAEQEAEAMQ
                     AKADEEVKIQEYLEANNITVEPTESGMYYIEHQQGSGPKPNQKDRVKVHYTGTLLDGK
                     KFDSSVDRDQPFEFVLGVGQVIRGWDEGIAMMNVGSKATFILPSSLAYGEHGAGRDIP
                     PYAPLIFEVELLDIVK"
     gene            complement(4751..5569)
                     /gene="cysQ"
                     /locus_tag="PK345_07385"
     CDS             complement(4751..5569)
                     /gene="cysQ"
                     /locus_tag="PK345_07385"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005776451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HPX34874.1"
                     /translation="MEKLLDTAILAAIFAGQELMINYNKTLSIDFKKDQSPVTNADRS
                     AHALIKTALQYFDIPLLSEEGEHLSYRIRKKWEKLWIVDPLDGTKEYIKRNDEFTVNI
                     AFIENNQPIIGVIYAPALQLLYFASKHLNKAFKHELKGGFTNLNELYYQAIELPINAH
                     LKTNPIVLASRSHLDLGTKGYIDGINKRCGKINLVSVGSSLKFGLIAEGSALLYPRFS
                     STYEWDTAAGQAIVEMAGGSVINADDGSPLIYNKKDLLNPHFITRSAFARSGLI"
     gene            complement(5616..>7543)
                     /gene="uvrB"
                     /locus_tag="PK345_07390"
     CDS             complement(5616..>7543)
                     /gene="uvrB"
                     /locus_tag="PK345_07390"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HPX34875.1"
                     /translation="LLGVTGSGKTFTIANVLNEVNRPALVLSHNKTLAAQLYGEFKQF
                     FPQNAVEYFVSYYDYYQPEAFIPSTNTYIEKDLSINDEIEKLRLSTTSSLLSGRRDVI
                     VVSSVSCIYGIGNPDDFTNNVIHLKVGQPIQRNNFLLSLVASLYSRNEVEFRHGQFRV
                     KGDVIDVFPAYADFAYRLVTWNNSIEELLSFDPITGKELEAYEQIVIYPANIFVTSKE
                     KMKSAILEIQQDMFRQVDFFRDNGKQLEAKRLEDRVSYDIEMMRELGYCSGIENYSRY
                     FDGRKAGQRPFCLIDYFPDDFILIVDESHVTIPQIRAMYGGDRSRKQTLVEHGFRLPS
                     ALDNRPLKFDEFEGITGQTIYVSATPAEYELMRSEGVVVEQVIRPTGLLDPVIEVRPS
                     LNQIDDLLEEIEIVVQRHERVLVTTLTKRMAEELYKYFTRINVKSRYIHSDIETLERV
                     KIMRQLRLGEFDVLVGVNLLREGLDLPEVSLVAILDADKEGFLRSERSLTQTAGRAAR
                     NINSRVIMYADTVTESMTKTINETNRRREKQLRYNEEYNITPTPITKSVESIMGQTSV
                     AGFETKQGKIYVENENIDVAADPVVKYMSRPEIEKAIQQARKKMEEVVKELDFIQAAQ
                     YRDEIYSLEAILKNIKE"
BASE COUNT         2261 a         1415 c         1534 g         2333 t
ORIGIN      
        1 tacaaatata aacattgatt acaaatgtaa attattaata attacaaaaa taaatacttt
       61 tatttttact tttgtaaaaa attaaaataa tggataagat acaagaaaga atatcattac
      121 tcatttcaac caagggaatg actaatactg aatttgcaga agcaattgat gtgcagcctg
      181 ctaatatttc acatgttatg tcggggcgta atcgtccaag tctcgactta gtaatgaaaa
      241 ttataacgag atttccagaa gttcgaaccg actggctttt atttggccaa ggtgctatga
      301 ataaagatta tactcttttc gatattgaag aaattccagt tgttcgaggt agggataagc
      361 aagaaaggcc ttttgagaaa ggagttgata gttatgaaac gagggaaata ctgacacaga
      421 aaggaattga agaagaggcg ttatcaagtg ttaatactgt caaaagagaa gaattttcag
      481 aaaaatcttt agaacctgat aatttcaacg ctggtcgcca ttctgatcaa aaaagcggca
      541 atctgacatc tgaggtaaga tccaaaaagg ttgagaaaat tattttgttt tataacgata
      601 aatctttcgg tgaatactat cctgaaagtg attaaatctg gattaatcat ttggccctca
      661 gtatctctat ttgcataaga aaattaattg gatagcttaa aagggggtct tcttgacaaa
      721 ttgtgattat aaaaatcaga ttcacaatac ttttcaatgc ggtttttata tttcaggagg
      781 gtaactgctc tgtgataata tacaaggtac tattctactt aagtctttct aaatgtgaga
      841 agaagaagtt ggattcaata attgcatttt catcgctatc cgagccatga actgcgttgg
      901 cctgaatgtt tgttccgtaa tcatacctta ttgtgccttt ttcagctttt tccggatttg
      961 tacttcctat cagtttacgg tattcttcta cggcattttc cttttccaat attgcggcaa
     1021 tgattggacc tgacgacata tattttacca gatcatcgta aaagggtttt ccagtatgga
     1081 taccataaaa ctccattgcc tgcgatttgg tgagttgtaa catcttcatc gcttttaact
     1141 taaatcctgc ttctaacact ctggtaatta ttttgccggc ataccccttt tccattgccg
     1201 taggcttgat cattgttaaa gtgatatttc ccttcataat gacatgatta tttgaattac
     1261 aaaaatagta aacatgaaat tgttacaatc atttgccctt tttatttagc tttgcagcgt
     1321 tttttacaat ctgtaatatt caacattcaa aacattgcct ccattgagcc agaatgatat
     1381 tgaaaaactc atcgagttat tcaaaaatcc aaaaaaaatt gtgatctcat cacattataa
     1441 tcctgatgga gatgctgtag ggtctgcaat ggcgttgtat catgttttaa agcagttgaa
     1501 tcatcaagta tcggtaattt ttcctaataa gtttccactt tttttgagtt ggatagaaga
     1561 gagtgaaaga agtctcattt tcgagcagga tggagcaagc gcttcgcaag tcataagcgc
     1621 tgccgatatc atcttttgtg ttgattataa ttcattcaaa aggttgggtg agatggaatc
     1681 gctgatggtt aaatcaaaag cgattaaagt acttattgat catcatcctg atcctgaaaa
     1741 agagcacttt gggtttgttt atcatagcgc ctcagcttct tcaactgctg agttagtgta
     1801 taagttttta aaactgacag ggctacatag caaaataaac cggcaggttg ctgaaagcct
     1861 gtatgcagga ataattacag atactggatc attgagtttc agttgtaata atcccgacac
     1921 atacattatc attgcgaaac tgatacaaaa agggttggac gctgaacgtg cgcatcggct
     1981 aatttatgac gacttttcgg aaaacaggat gcgtttgctt ggatatacca tctaccagaa
     2041 aatgattgtc atacccgagt atttcactgc gatcattgca cttacaatag atgaacttgc
     2101 acggtttggg tatcagcagg gcgatactga aggcattgtg aattttccgc tttcgatgaa
     2161 agctattaat ttttcggtga tgctggttga acgtacagat agaatcaggt tgtcatttag
     2221 gtcgaaaggt agcttccctg caaatatcat tgctgcacgc tatttcgaag gtggaggaca
     2281 tcttaatgcc gcaggcggcg attcttattt gtcaatgcaa gagacaataa aaagattaaa
     2341 ggagatcctt cctcagtatt atgatcagct caattttgaa atcagataag gaaaatgatg
     2401 attaacatgt ttaaaatatc aatggttatc ttgttaccct ttttgttgtt tgcgtgtcat
     2461 caatcgcaac cgagggaatc tttaaatcaa gagcctgcaa atctcaaaga ggagttgatt
     2521 gaagccaata aaggcacagt gaagacagaa gatcagcata ttgaagattt tatcaatcgg
     2581 cgtggatgga aaatgcagac ttcaggtaca ggactaagat atatgatcac tacaaggtgt
     2641 aatggaaaga aggctgtaaa tggggatatc gctgttttgg aatataaaac aagacttatt
     2701 accggcgaag tgatttattc atcagataac gatgggttaa aagagtttga agtgggccat
     2761 ggtggggtag aagcagggtt agaagaggcc gtttgtatga tgcaaatcgg cgataagggt
     2821 tatttcatct taccttccta tattgcttgg ggattatcgg gagacggcca actgatccct
     2881 cccagatcaa cattgattta tgaattaaaa ctaatagatt tgaaataatt aagaacataa
     2941 ttttatggta aagaaatctt tttttataat cactaccctt ctcttggcgg taattcttat
     3001 aagcgcctgc gggcgtagta gcgacggttt taaaacaacc tcttcggggc ttaaatataa
     3061 gtttcacgta aaaggtgaaa aagataccct gaatgttaat ctttatgata ttgtatatgt
     3121 tagaatggat tacagggtag ccgacacaat gttgtttcaa agtggtgtta ctccaatatc
     3181 ctttcaggta aagcctggcg atgtaggaga tttgcaggag ggtttgttga tgatgaaggt
     3241 tggcgatagt gcaacatttg ccatgcctgc cgagaagttt tttattaatt tgatgaaata
     3301 caaagaggta ccggcagttt tcagtgatta tgcagagttg tacgttgaca taaaagttgt
     3361 tgccttgata ccagagcctc aggctttgaa agatgaacgt gcagctgccg aagtgcgtaa
     3421 agctaccgaa caggacgtca tcaaaaaata tattgaagaa caaaatatca ccgtacagcc
     3481 tactgccaat gggctttatt atatcgaaat agtaaaagga aaaggcaaaa aggctgaggc
     3541 tggtaatcgt ctaaaagtac attataaagg ccttttattc aacggtgagg ttttcgattc
     3601 ctcgtacgac cgcaacaagc cagttacttt taccttaggt aatggagaga ggctaccagc
     3661 atgggacgaa gggtttgccc tgatggctgc cggtggtaaa gcacgcttca ttgtgccttc
     3721 ggcccttggt tatggtgccg aacagagaag caaagtaaag ccatatacac cacttatttt
     3781 tgaagtagaa ctacttgaag ttactgatta atcgagcatt ataattcaat aataattatt
     3841 cactatcaaa ttacattttt tatgaaagca aaattcacaa tgttctttac tcttttggtc
     3901 atcatgggaa catttatgat ctcgtgcaag acaaggaaag ccgattacaa gtttaacgaa
     3961 aacggaatcg gatatctgtt ccacactaaa aattccaatg ctcaaatgcc ggaaaacgga
     4021 gacattttaa gtatgaaact cgcttatgga gcgcctgata gcgtctattt tcatactgac
     4081 tccattcctg agaagaaaat gatattaccg atggttacgt ccgagcatgc cggagacctc
     4141 tatgaagctc ttgcaatgat gcaccttggc gatagtgtat cttttctatt gcccgccgaa
     4201 acttttttct tgaaaaccgc tcatttccct gaaattccat tttttgcaaa aggagtggat
     4261 aagttgctgt tcaacattaa actcgataaa atacagacag aagagcagat gtatgctgaa
     4321 caagaggccg aagcaatgca agcaaaggct gatgaagagg tgaaaatcca agagtattta
     4381 gaggctaaca atataaccgt agaacctacc gaatcgggga tgtattacat tgaacatcag
     4441 cagggcagcg gtcctaaacc caatcaaaaa gatagggtaa aggttcacta cactggtaca
     4501 ctgctggatg gcaagaagtt tgactcttct gtagatcgtg atcagccatt tgaatttgtc
     4561 cttggtgtgg gtcaggtaat tcgtggttgg gacgaaggta ttgctatgat gaacgtaggt
     4621 tcaaaagcta cttttattct gccttcatcc cttgcctatg gcgagcatgg cgccggacgc
     4681 gatatcccac cttatgcacc gctaattttt gaagtggaat tgctcgatat tgtcaaatag
     4741 tatttatgta tcagatcaat cctgaccgtg cgaaagcgct tcgggtgata aaatgaggat
     4801 ttaacagatc ttttttatta taaatgaggg gggagccatc gtctgcattg atgacggagc
     4861 ctcctgccat ttctactatt gcctgaccgg ctgcagtatc ccattcataa gttgatgaaa
     4921 aacgcggata tagtaaagca gagccttcag ctattaagcc gaactttaac gaactgccaa
     4981 cagaaacaag attgattttg ccacaccttt tgttaatacc atcaatatat cctttagttc
     5041 ccaaatcaag atgagatctg ctggcaagca caatgggatt tgttttcaaa tgcgcattta
     5101 ttggtagctc aatggcttga tagtataatt cattaagatt tgtgaaacca cctttcaatt
     5161 catgtttaaa agccttatta agatgctttg acgcaaaata gagcagttgt agtgcaggag
     5221 catatattac tccaattata ggttgattat tttcaataaa tgcaatgttt acagtaaact
     5281 cgtcattgcg tttgatatac tccttggtac catccaacgg atctacgatc caaagttttt
     5341 cccatttttt tcggattctg tagctcagat gctcaccctc ctcactaagc agagggatat
     5401 caaaatattg caatgcggtt ttaattagtg cgtgagcaga acgatcggca tttgttactg
     5461 gactttgatc ctttttaaaa tcaatgctca acgttttgtt ataattaatc attagctctt
     5521 gccctgcgaa aatagctgct aatatggcgg tatccaataa cttttccata ttaaaatcat
     5581 tatccatagg ggattattaa atgatgaggt agattttatt cttttatgtt ttttagtatg
     5641 gcttcgaggc tgtatatctc gtcgcgatac tgtgctgcct gaataaaatc aagctctttt
     5701 actacctctt ccattttttt gcgtgcttgc tgtatggctt tttcaatctc agggcgcgac
     5761 atatatttta ccaccggatc ggcagcgacg tcaatatttt cattttctac gtaaatcttg
     5821 ccttgtttag tttcaaagcc tgcaacagag gtttgcccca ttattgattc gactgacttg
     5881 gttatcgggg taggcgtaat attgtactct tcgttgtatc gaagttgctt ttcacgtctt
     5941 cggttcgttt cgttaattgt tttggtcatt gattcggtta ctgtgtcagc atacattatc
     6001 actcttgagt tgatattacg cgcagcacgt cccgctgttt gggttaggga tctttcggaa
     6061 cgcaaaaaac cctctttatc agcatcgagg attgccacca gcgatacttc aggcaagtca
     6121 aggccttcgc gcaacaaatt tactcctaca agcacatcga actcacctaa acgcaactgg
     6181 cgcattatct ttactctttc aagcgtttca atatcagagt ggatatatcg gctcttaaca
     6241 tttatccgtg taaaatattt atatagctcc tctgccatcc ttttggtcag agtagtaaca
     6301 agcacacgtt cgtggcgttg taccacgatt tcaatttctt caagtagatc gtcaatctga
     6361 ttcaggcttg gtctaacctc aatcactgga tcgagcagtc cggtaggacg tatcacttgc
     6421 tctacaacca ccccttcgct gcgcatcaat tcgtattcag caggtgtggc gcttacgtaa
     6481 atcgtttgtc cggtaatacc ttcaaattca tcaaatttaa gcggtctgtt atcaagcgct
     6541 gacggtaatc tgaagccatg ctctacaaga gtttgtttac gcgatcggtc gccgccatac
     6601 atagccctga tctgcgggat agttacatga ctttcgtcaa caatcaagat aaagtcgtca
     6661 gggaaataat caataaggca aaagggacgc tggccggctt tgcgcccgtc aaaatatcga
     6721 gagtaatttt caatacccga gcaatatcca agctctcgca tcatttcaat atcataacta
     6781 acgcgatctt cgaggcgttt ggcttctaat tgtttaccgt tatcgcgaaa aaaatcaact
     6841 tgcctgaaca tatcctgttg tatctctaat attgccgact tcattttctc tttggaggta
     6901 acaaaaatat tggcaggata tatcactatc tgttcatagg cctccaactc tttgcctgtt
     6961 atcggatcga agcttaacag ctcttcaatg ctgttgttcc atgttactaa cctatatgca
     7021 aagtcggcat aagccggaaa gacatctatc acatcgcctt tcaccctaaa ttgaccatgc
     7081 cggaattcaa cttcgtttcg cgaatatagc gaagctacaa ggctaagtaa gaagttatta
     7141 cgttgaatcg gctggcctac tttcagatga ataacgttgt tggtaaagtc gtcgggattg
     7201 ccaataccgt agatacagct tactgatgat acaacaatta catctcttcg acccgataat
     7261 aacgaagagg tagtgctcaa tcgtagcttt tcaatctcat catttatcga aaggtctttt
     7321 tcaatatagg tatttgtgct tggaataaag gcttcgggtt gataataatc ataataagag
     7381 acaaaatact caactgcgtt ttgtggaaaa aactgcttaa actcaccata aagctgtgcg
     7441 gctaacgtct tgttatggct aagcactaac gccggcctgt ttacctcgtt cagcacattg
     7501 gcaatggtaa aggtcttgcc cgaccctgta acgccaagca aaa
//
LOCUS       DAOOAI010000102         7541 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_11716_length_7541_cov_5.259551, whole genome shotgun sequence.
ACCESSION   DAOOAI010000102 DAOOAI010000000
VERSION     DAOOAI010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7541)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7541
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11716_length_7541_cov_5.259551"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..486)
                     /locus_tag="PK345_07395"
     CDS             complement(334..486)
                     /locus_tag="PK345_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34876.1"
                     /translation="MIIRNIEFKHKKDALVYFKNIVNSYKPIQTINENDFKDLVELIE
                     NHPDKE"
     gene            798..1793
                     /locus_tag="PK345_07400"
     CDS             798..1793
                     /locus_tag="PK345_07400"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase family protein"
                     /protein_id="HPX34877.1"
                     /translation="MIYLAPMQGYTEAEFRLAWAMFFSGIDVAVSPFIPLSEGIVFRA
                     KHLNDIMPGRNQKLAVVPQVLGNNSEQIIRLARRIADLGYETINWNLGCPKISVAKKQ
                     RGSGLLPFPNKIRSILDQVIPNIPVQLSIKTRLGFQSPDEFYELLPVYNDYPLQSLII
                     HPRIGSQMYEGNLFLEVLDQTINLVKHTIVFSGDISNIRSFEALRKRYPTIEHWMLGR
                     GVLVNPFLPEIILEGSRHLDEATIRHKQYYFAEELYCGLKDKLRNEHTVLNKLKDYWS
                     YFARWFTGEQQVFDTLSRLHSLKEFIHVSNIVLRNNPLASFETRIARPVGKGIQV"
     gene            1949..3736
                     /gene="lepA"
                     /locus_tag="PK345_07405"
     CDS             1949..3736
                     /gene="lepA"
                     /locus_tag="PK345_07405"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008862039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HPX34878.1"
                     /translation="MNNIRNFCIIAHIDHGKSSLADRLLDMTETLTERTKKDQILDNM
                     DLERERGITIKSHAIQMVYHFNNKNYILNLIDTPGHVDFSYEVSRSIAACEGALLIVD
                     ATQGIQAQTISNLYLALDHDLTIIPVLNKVDLEQAMIEEVKDQIVDLLGCNRDEIIEA
                     SGKTGYGVEEILEAIVDRIPEPQGDPEAPLQALIFDSVFNSYRGIIAYFRIFNGTIRQ
                     NDFVKFMATGKEYDADEVGVLKLGYSPREELKAGDVGYIISGIKVLAEVKVGDTITHV
                     ENPSHTAIAGFEHVKPMVFAGIYPVDADDYEELRASIEKLQLNDASLTFEPESSAALG
                     FGFRCGFLGLLHMEIVQERLDREFDMDVITTVPNVSYKAYTKKGEMLEVHNPSGLPDP
                     NYLDYIEEPYIIAQIISKTEFVGAVMKLCIDKRGVLKNQIYLTSERVEITFELPLAEI
                     VFDFYDKLKSISRGYASFDYHISEYKIAKLVRLDILLNGERVDALSTLIHQDNAYTFG
                     RKMCEKLRELIPRQQFDVAIQAAIGSKIIARETVKALRKDVTAKCYGGDITRKRKLLE
                     KQKKGKKRMRQVGNVEVPQSAFLAVLKLD"
     gene            3806..4549
                     /gene="rlmB"
                     /locus_tag="PK345_07410"
     CDS             3806..4549
                     /gene="rlmB"
                     /locus_tag="PK345_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005937581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HPX34879.1"
                     /translation="MRKNDNFIFGIRPVIESIRAGKEFDKVFIQNGLKSDQASELFTL
                     LKDNQIHFQYVPLEKLNRITRKNHQGVVAFTSLVSYQPIDEVVQMAFERGEAPLLIIL
                     DRVTDVRNFGAIARTAECAGVHGIIVPAQNSAQINADAMKTSAGALNFISISKVGDLS
                     KTIDNLKKSGLQIVATYEKASETYTNIDLTIPTAIIIGSEEDGIAPALLKKADREVKI
                     PIIGRTASLNASVAAAIIIYEAIRHRTKQ"
     gene            complement(4662..6110)
                     /gene="nhaC"
                     /locus_tag="PK345_07415"
     CDS             complement(4662..6110)
                     /gene="nhaC"
                     /locus_tag="PK345_07415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HPX34880.1"
                     /translation="MVRLKRQPSLIEALFPVFFLTIFISINVFIFGEDSLSGSNQIVL
                     LLSAAVAALIAFRTGVSWEALNDGIVKSVGSAMSAILILLLIGSLAGTWLLSGIVPSM
                     VYYGLKILNPKIFLFASCIVCSIISLATGSSWSTVATLGVALLGIGRALGLPDGLIAG
                     AIISGAYFGDKMSPLSDTTNLASAMAEVDLFKHIRYMTLTTTPSLIITLILFLVIGIT
                     RGEATELTDIQPILAALDSTFNINLLLFLVPALVIFMIIKKVPAIPALLTGTLAGGLF
                     AVIFQPQIIEQVSGVEGKFHLASYVAIMKSMYTNISIKTDHEMVNNLLQTGGMRGMLN
                     TVWLIMCAMVFGGVMESAGLLKRITQGVITLVRSQGSLIASTAGTCVFFNITASDQYL
                     SIVVPGRMFADTFKEYNLAPQNLSRTLEDSGTVTSVLVPWNTCGATQSTVLGVSTLIY
                     FPYCFFNLISPLMTILYGYLNIRIEKLKSQKS"
     gene            6322..7095
                     /gene="tpiA"
                     /locus_tag="PK345_07420"
     CDS             6322..7095
                     /gene="tpiA"
                     /locus_tag="PK345_07420"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007655752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HPX34881.1"
                     /translation="MRIKIAAGNWKMNTTLEEGRRLINAITEGLLNNPSPLSPNELQV
                     VFGVPFITLESAVDMTKEQKQIKIAAQNCCSEEKGAFTGEVSAAMIRSTGAQCVIIGH
                     SERREYFNESHAMLAKKVDVALKNNLIPIFCCGEKLEIRHKNDHFDLVKKQIEESLFH
                     LNPDLFAHVVIAYEPVWAIGTGVTATPQQAQEMHNVIRNFINKRYGNSISNSTTILYG
                     GSVNAGNAGELFSQPDVDGGLIGGASLKADEFLKIIHSF"
     gene            7222..>7541
                     /locus_tag="PK345_07425"
     CDS             7222..>7541
                     /locus_tag="PK345_07425"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006799498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPX34882.1"
                     /translation="MLLASQNKYPTDYFGSPIDFPISLSGTFGELRNGHLHSGIDIRT
                     QGVEGKPIKAIADGWVSRVVVSPTGYGKAIYIDHPNGYTSLYGHCQSFYQAIEKYVNT
                     EQYR"
BASE COUNT         2423 a         1519 c         1444 g         2155 t
ORIGIN      
        1 ttgcaacaac ttattataaa tctagtcatc gcaaaaagca aaataataat tagagtcttt
       61 tttatcatat cttattgttt tagcgtttga tctcaatatt ttagagtaac gagtatttta
      121 tctgttttct ttattcatcg tcacatttct taaatcttcg ctaattgttt cgctacaagt
      181 ctttctaaat tttgcaagag gatttgattc accattaatg cgctttcgat aggaaaatac
      241 ttccgttgaa ccatcctttc ttattagttc aaaatatttt gttttatatc taacttcaat
      301 tacctgattt tttttaattc cgcatacaat tttttactct ttgtcaggat gattctcaat
      361 taattcaact aaatctttga agtcattttc attaatagtc tgtattggct tatatgagtt
      421 tacaatattc ttgaaataga ctaaagcgtc tttcttatgt ttaaattcta tatttctgat
      481 tatcattttg caagctcgtc tttttctaaa ttaatgctaa caaatgggta acgtaataat
      541 accttaaact attgaagcgt agccggtttt gtaattttgt acaaacataa tggtttattt
      601 aaacctttcg caagcgattc caaaaaattt tttaccattt tcgctctctt tcaaaagcca
      661 tgtttttggt ctgtgttttt ataatatttc cgcaatattc cgattggttt gtacgaaata
      721 gtggcagtat ctattttcta ttttctattt acttttgatc tccaaaagaa aatcgaattt
      781 tcaatcattt gcaaattatg atatatcttg ctccgatgca aggatatacc gaagctgagt
      841 tcaggttggc ttgggcgatg tttttctccg ggatagatgt tgcagtaagt ccctttatcc
      901 ctttatccga aggcattgtt tttcgggcta aacacctgaa cgatatcatg cccggtagaa
      961 accaaaaatt agcggtcgta ccacaggttt taggaaataa ttcagaacaa atcattaggc
     1021 ttgctcgccg gattgctgat ttaggctacg aaactataaa ctggaattta ggctgtccta
     1081 aaatttcggt agccaagaaa caacgtggtt caggactgtt acctttcccc aacaagatca
     1141 gatcaatact cgatcaggtt atacccaaca ttcctgtaca actctctata aaaacacgat
     1201 taggctttca atcgccggat gaattttacg aacttttgcc cgtttacaac gattatcctt
     1261 tacaaagcct gattatccat ccgagaatcg ggagtcagat gtacgaaggt aatctctttt
     1321 tagaggtttt agatcaaacc ataaacctgg ttaaacacac catagttttt agcggcgaca
     1381 tttccaacat cagaagtttt gaagctttgc gaaaaagata tccaacgatt gaacactgga
     1441 tgttaggtcg gggggtttta gttaatccat ttttgcctga aataatatta gaaggatccc
     1501 ggcatttaga cgaagctaca atacgccaca agcaatacta cttcgcagaa gagctttact
     1561 gcgggctgaa agataaactc cgaaatgaac atactgtttt aaataaactg aaagattatt
     1621 ggtcatattt cgcaagatgg ttcaccggcg agcaacaagt tttcgatact ctatcaaggc
     1681 ttcattcatt aaaagagttc attcatgttt caaatattgt tttacggaat aatccattgg
     1741 catcgttcga aacaaggatt gcaagacctg ttgggaaagg tattcaagtg taggtaatct
     1801 gcaccgcggc aatctctact gaacaaacac taaatctgga ttttcatcat aacatcaata
     1861 aactatttca aaaaaaaact ctgctttgat tgctttggca taaagaaaaa actgagatgt
     1921 tatttcattt tttctaattt tgccgactat gaataatata cgaaactttt gtattatcgc
     1981 ccatatagat cacggtaaaa gttcgcttgc cgatcgtttg cttgatatga ccgagacact
     2041 caccgagcgt actaaaaagg atcagatact tgataatatg gatttggagc gcgagcgcgg
     2101 cataaccatt aaaagccacg ctatccagat ggtttatcat tttaataata aaaactacat
     2161 actaaacctg attgacactc ccggccacgt ggatttttcc tatgaagttt cgcgttcaat
     2221 agcagcctgc gaaggcgcat tactaattgt agatgccacc caaggaatac aggcacaaac
     2281 aatatctaat ctctatctgg ctctcgacca tgatcttaca ataattccgg ttctcaacaa
     2341 agttgacctt gaacaagcaa tgattgaaga agtaaaggat cagattgtgg acctgttagg
     2401 ctgcaatcgg gatgaaataa tcgaggcaag cggcaaaaca ggctatggcg tagaagagat
     2461 tcttgaagca atagtagatc gtattcccga gccacaaggc gaccccgaag ctccattgca
     2521 ggcgctgatt ttcgattcgg tttttaactc atacagaggg atcattgcct atttcagaat
     2581 atttaatggc acaatacgcc aaaacgattt tgtgaagttt atggctaccg gtaaagaata
     2641 tgatgccgat gaagttggag tgctgaaatt aggctactcg ccaagagaag agctaaaagc
     2701 gggagatgtt ggatatatta tttcgggaat taaagtttta gctgaagtaa aagttggcga
     2761 cacaataacc catgtagaaa acccaagcca tactgccatt gcaggtttcg aacatgtgaa
     2821 acctatggtt tttgccggaa tatatcctgt agatgccgat gattacgaag agttacgcgc
     2881 ctccatagag aagctgcaac ttaatgatgc ctcgcttact ttcgaacctg aatcatcggc
     2941 agccttaggc tttggttttc gatgcggatt tcttggactg ctgcacatgg agatagtaca
     3001 ggagcgcctc gacagagagt tcgatatgga tgtgataacc acagtgccaa acgtctctta
     3061 taaagcctac acaaaaaaag gagaaatgct cgaagtgcat aacccatcag ggcttcccga
     3121 tcccaattac ctcgattata tcgaagaacc atacataata gcgcaaatta tctctaagac
     3181 tgaatttgtt ggagcagtga tgaagctttg tattgataaa agaggagtat taaaaaatca
     3241 aatatacctt accagcgaac gggttgaaat cacttttgaa ttgccacttg cagagatcgt
     3301 ttttgatttt tatgataaac taaaaagtat atcacgcggt tatgcctctt tcgactatca
     3361 tatttcggaa tacaaaatag caaagcttgt aaggctcgat atattgctta atggagagcg
     3421 ggttgatgca ttatcaacgc ttatccatca ggataatgcc tatacctttg gccgtaaaat
     3481 gtgcgaaaaa cttagagaac tgatacctcg ccaacaattt gatgtagcaa tacaagctgc
     3541 cataggttca aagatcatag ctcgtgaaac agtaaaagca ttgcgtaaag atgttacagc
     3601 caaatgttat ggaggcgaca taaccagaaa gcgtaaattg cttgaaaagc agaaaaaagg
     3661 aaaaaaacga atgcgtcaag tagggaacgt tgaagtgcca cagtcagcct tcctggctgt
     3721 attgaaatta gattaaaaga taattcagac aggataagac gctatttaca aaacttcccc
     3781 gctcccacct aatgacaatt tcaatatgag aaaaaacgac aactttattt ttggtatccg
     3841 accggtgata gaatctatca gagctggaaa agagttcgat aaagtgttta tacagaatgg
     3901 attgaaaagc gatcaggcct ctgagctatt tactctttta aaggataatc agatacattt
     3961 tcaatatgta cctctcgaaa aactaaatcg tatcacacgc aaaaatcacc aaggagttgt
     4021 cgcttttact tcattggttt cgtaccagcc cattgatgag gtagtacaaa tggcttttga
     4081 acgtggtgaa gcgcccctgc tcattatcct cgacagagtt accgacgtca ggaacttcgg
     4141 agcgatagca cgcactgccg agtgtgcagg cgtacacggg ataatcgtgc cggctcaaaa
     4201 ctccgcacag ataaatgccg atgccatgaa aacctcagcc ggagctttga attttatttc
     4261 catctccaaa gttggcgatc ttagcaaaac cattgataac ctgaaaaaaa gtgggttaca
     4321 gattgttgcg acctacgaaa aggcgtcaga aacatatacc aacattgact taaccatccc
     4381 taccgctata attataggat cagaagaaga tggaatagct cctgcgctac taaaaaaagc
     4441 tgatcgcgag gtaaaaattc caatcatagg ccgcacagct tctctaaacg cctcggtagc
     4501 cgcagcaata ataatttacg aagcaatacg gcataggacg aagcaataaa taaacaagtt
     4561 gtagctaaaa tcccaccttc gaattattta gcctcgaata caagtgttta ttaatgtatt
     4621 tgtaccaagc tatttgtctc tattcattca ctaccttgat atcagctttt ctggcttttg
     4681 agtttctcaa tcctgatatt cagatagccg tataaaattg tcatcagcgg acttatcaga
     4741 ttaaaaaagc aatagggaaa atatattaat gtggaaacac ctaatacggt tgattgtgtg
     4801 gcgccgcatg tattccatgg cactaagaca gatgttaccg ttccactatc ttctaatgtt
     4861 ctggataagt tttgcggagc taaattgtac tctttaaaag tgtcggcaaa catacgtccg
     4921 ggtactacaa tactgagata ttgatcgctg gccgttatat tgaaaaacac gcaagttcct
     4981 gctgttgaag caatcagtga accttggctt ctaacaagtg ttataacacc ctgtgtgatc
     5041 cgtttaagta atcctgccga ttccattact ccaccaaaca ccatagcgca cataataagc
     5101 catacagtgt taagcatacc gcgcattcca ccagtttgca acaaattatt caccatctca
     5161 tgatcagtct taatgctgat attggtgtac attgatttca ttatcgccac ataggaggca
     5221 agatgaaatt taccctcaac accactgacc tgctcaataa tttgaggctg aaaaatcacc
     5281 gcaaagaggc cgcccgcaag cgtaccggta agaagtgcag ggatggcggg aacttttttt
     5341 atgatcataa aaataacgag agcagggacg aggaagagta atagatttat gttaaaagta
     5401 ctatctaaag ctgcaagtat cggttgaata tcggttagtt cggttgcttc gccacgtgta
     5461 atgccaataa ccagaaacag aattaaagtg atgatcagcg atggcgtggt ggtaagggtc
     5521 atataacgga tatgtttgaa cagatcaact tcagccatag ccgatgcaag gtttgtagta
     5581 tcggatagag gcgacatttt atcaccgaaa taagcgccgg agataattgc accggcaatc
     5641 aagccatccg gcagccctaa cgccctgcca ataccaagca aagctacgcc aagggtggct
     5701 actgttgacc aactgctgcc agtagccaac gaaataattg aacaaactat gcaagaggca
     5761 aaaagaaaaa tcttaggatt caaaatcttt aatccgtaat aaaccatcga aggaactatt
     5821 ccactcaaaa gccacgtacc ggccaatgac cctatcaata gcagaatcag tattgccgac
     5881 atggccgatc ccacgctttt tacaatgcca tcattaagcg cttcccagct aacgccggtt
     5941 ctaaatgcaa tcaaagcagc tacagctgcc gatagtagca atactatctg gttagagccg
     6001 gaaagcgaat cttcaccaaa aatgaaaaca tttatggaaa taaaaatcgt aagaaaaaac
     6061 actggaaaga gcgcctcaat caaactgggc tgccgtttta atcttaccat aattatcaaa
     6121 ctcaaattga gaatacaaac atagggataa agaaaaaaat aattttttaa atgggtattt
     6181 taaaagaaat ttaaaagaaa aaaccgctac ctccaataac tcaaaccctt gtcaattgcc
     6241 gataatccat cttatcacat tctacacccg taattaaccg cttttcctat ctttgctgaa
     6301 aatttataac acaatagtat tatgcgaata aaaattgcag cgggcaactg gaagatgaat
     6361 accaccttag aagaaggtag aaggcttatc aacgccatta ccgaaggttt gctgaataac
     6421 ccctcccctc tttcacctaa cgagttgcag gtggtgtttg gagttccgtt tatcacactc
     6481 gaaagcgccg ttgatatgac caaagagcag aaacagatca aaatagcagc acaaaattgt
     6541 tgtagcgaag aaaagggagc tttcaccggc gaagtgagtg cagcaatgat cagatcaacc
     6601 ggcgcccagt gcgtaatcat aggtcattcc gaacgaaggg aatattttaa cgaaagccat
     6661 gcaatgctcg caaaaaaggt ggatgtagcg cttaaaaaca atcttatccc gattttttgc
     6721 tgcggcgaaa aattagaaat acgtcataaa aacgatcatt tcgatttggt aaaaaaacaa
     6781 attgaagagt ccctatttca tcttaatccc gatttatttg cacatgtggt tatcgcttat
     6841 gagccggttt gggcaatcgg aacaggagtt acagctactc cccaacaagc acaagagatg
     6901 cataatgtta tccggaactt tataaacaaa agatacggca acagtatttc aaacagcacc
     6961 accattttat atggaggctc agtgaatgcc ggtaatgccg gcgaactctt ctcgcaaccg
     7021 gatgttgatg gcggacttat tggcggcgca tcgttaaagg ctgacgaatt tctcaagatc
     7081 atccactcat tttaagctaa aaacctttga tctcttttca ataaaaagcc tgcattcaat
     7141 ggcttttaag tcatattgtt actttttgaa cttatgagct ttataaaaaa aatactttct
     7201 cttgttctta ttgttcaacc catgttactg gcgagccaaa ataaatatcc taccgattat
     7261 tttggatcac cgatagattt tcctatttct ctgtcaggca cttttggcga gctacgtaac
     7321 ggacatctgc actcaggtat tgatatccgc acacaaggcg ttgaaggtaa accaataaag
     7381 gctattgccg atggttgggt atctcgcgtt gttgtgtcgc ctacaggata cggtaaagcg
     7441 atttacatag atcatccgaa cggctacacc tctctgtatg gtcattgcca aagtttttat
     7501 caggctatcg aaaagtatgt taacaccgaa caatatcgta a
//
LOCUS       DAOOAI010000103         6985 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_12860_length_6985_cov_4.968110, whole genome shotgun sequence.
ACCESSION   DAOOAI010000103 DAOOAI010000000
VERSION     DAOOAI010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6985)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6985)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6985
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12860_length_6985_cov_4.968110"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="PK345_07430"
     CDS             <1..362
                     /locus_tag="PK345_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34883.1"
                     /translation="ELNQINLFYDLRSQMDKLLQDKTPIARMEIKHIKEMISNLFIDK
                     SSQEFHKALKAEFPKLTFDDLRLCSYLKHRFLNVEIAEFLETSPEAVYTRKSRLRQKL
                     NLEKNQDINDFLSNYTY"
     gene            complement(374..949)
                     /locus_tag="PK345_07435"
     CDS             complement(374..949)
                     /locus_tag="PK345_07435"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008144792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HPX34884.1"
                     /translation="MNPLLTTEIKNAVEVLASGGTILYPTDTIWGIGCDATNQKAVDK
                     IYKIKRRMESKSLLMLVESPDRIFDYVEKVSPLFIDLIKSYDKPLTVIYQGAKNVAKG
                     VAAGDNTIGIRVTKDEFCKELISAFGKAIVSTSANISGEPSAIRFQQISEKVKSQVDY
                     VVKLYQNEIRLMRPSRIIRITETGDFEIVRE"
     gene            complement(983..1996)
                     /locus_tag="PK345_07440"
     CDS             complement(983..1996)
                     /locus_tag="PK345_07440"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HPX34885.1"
                     /translation="MKKRIIHRILIIQTASIGDVTLATPLIEKLSDFFPKAKIDFLLK
                     KGIESLLNGHPMLNRVIVFDKSNKKFENIYRLIRQIRKEEYDVVINLQRFASTGLITA
                     FSAAKIKLGFRKNPFSIFFTKAVRHRISANPAKSAHEVSRNLSLIDFITDNNHNYPVR
                     LYPSKNNYAKMSQYKTHFYLTISPTSLWFTKQFPAEKWIAFLKRVDKEQYIYFLGSKN
                     DFATAEHIIVSSGHTNSINLCGKLSFLESAVLMRDAKMNFVNDSAPLHFASAMNAPVT
                     AMFCSTVPAFGFGPLSDDAVIIEVDDKINCRPCGLHGYPKCPKDHFKCALDIKIEKLL
                     KRC"
     gene            complement(2012..2827)
                     /locus_tag="PK345_07445"
     CDS             complement(2012..2827)
                     /locus_tag="PK345_07445"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009598679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HPX34886.1"
                     /translation="MERLIKIIESAWKNRDLLKDAEVIKSIEYVVELLDKGKLRCAEP
                     SDSGWKVNDWVKKAVILYFPIRNMETTEAGILEFHDKIKLKQNYKKYGIRVVPHAVAR
                     YGAFLGRGVVMMPSYVNIGAYVDCGTMIDTWATVGSCAQIGKNVHISGGVGIGGVLEP
                     VQASPVIIEDNCFIGSRSILVEGVRVEKEAVIGANVVITQSTRIIDVTGESPVEYKGF
                     VPARSVVISGTFPKSFPSGIYQVACALIIGKRKPSTDLKTSLNKALRDYDVPV"
     gene            3038..3466
                     /gene="ruvX"
                     /locus_tag="PK345_07450"
     CDS             3038..3466
                     /gene="ruvX"
                     /locus_tag="PK345_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HPX34887.1"
                     /translation="MGRILAIDYGQKRVGLAVTDEMQIIATGLDTVHVNDLFAYLEKY
                     MEGKSIEKIVVGEPRNLDNTPSDSQRFIEPFIKKLKKHFPDIPVFRVDERFTSKMAKD
                     VILSSGIGKKARQKKELVDKISATIMLQSFMQQQLMKEKY"
     gene            3477..4043
                     /gene="def"
                     /locus_tag="PK345_07455"
     CDS             3477..4043
                     /gene="def"
                     /locus_tag="PK345_07455"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006843791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HPX34888.1"
                     /translation="MILPIVAYGHPVLKKMSEEIQQDHPGLNELINNMFETMYSSGGI
                     GLAASQVNHSIRLFIIDANPMAEDFPELKGFKQVFINPYILEESNEIVSAEEGCLSIP
                     NIRESVPRPNEIVLEYYDNDWNLVEQRFKGYAARVIQHEYDHVEGILFVEKISPLRKT
                     LISRKLKEISIGKVDVDYKMIFPQKKRN"
     gene            4082..4624
                     /locus_tag="PK345_07460"
     CDS             4082..4624
                     /locus_tag="PK345_07460"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HPX34889.1"
                     /translation="MIRRSFFYITFVATILFLASCQSSRTKAVKLVQTMEDSVFGSTG
                     AIDRVKVGELIDLYINFADKFSKDSLAPVYLFKAADMAMNTGRSLEAIQLYERILDNY
                     SEYSKAPEALFLMGYVYENNLGELAKAEEIYKKFLELYPDNDFADDAEISLKYLGKSP
                     EELIEIFQQQGVFSGFGEEN"
     gene            complement(4638..5450)
                     /gene="cdaA"
                     /locus_tag="PK345_07465"
     CDS             complement(4638..5450)
                     /gene="cdaA"
                     /locus_tag="PK345_07465"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HPX34890.1"
                     /translation="MANFFITAFIQIKLADLIDILLVAVLIYMLYNLLKGTAATNILI
                     GIFAIFIFWRVVLLLEMEMLSQILGAFISVGFIALIVVFQPEIRQFLLLIGTPRFLVN
                     MRRRFGFLKFKTKMIFILDVDPIVSACRKMSVTKTGALIVIARLNELQQYTESGQVID
                     AAISEDLILNLFFKNSPLHDGAVIVLNNRIRSARVILPVSGNQNLPGEIGLRHRAALG
                     ITEKTDAVAIVVSEEAGYISCFQRGNVKLNITPSQLKNFLDSEFNLEEKTEK"
     gene            5600..6217
                     /locus_tag="PK345_07470"
     CDS             5600..6217
                     /locus_tag="PK345_07470"
                     /inference="COORDINATES: protein motif:HMM:NF014269.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HPX34891.1"
                     /translation="MSEETKHNFPFFPLNLIVMPGEIQPLHIFEPRYKQLIHDIQTSG
                     EHFGIPFMLNNKICKLGSCVKLYKILSETSTGEMDILVKGVSIISIESMEDTLGEKLY
                     SGGSVKILKELNKTPSTELSNEFKNYQAQLAKINKIEGNSEKTNEPLNVLDIAGQLPL
                     EIEEKFNFLILNCPIKRETFLLNKIKFLHMINQKLEQLGYRFFLN"
     gene            complement(6441..>6985)
                     /locus_tag="PK345_07475"
     CDS             complement(6441..>6985)
                     /locus_tag="PK345_07475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34892.1"
                     /translation="MIEDDNNKSSSVHSLSNTKSNNSNYFFEGKTEIIRSKVSNTYLV
                     FFEKSARIFELVIVYLNNFIRILTSQKFSEVAFEVVVSGQNNLIPCGLAKRSNVNVQL
                     FSVALNYLSGNPGRDHLLLKSLNESVKAILQSVDNLLSDLLTKINKIRYHLINKNPFT
                     LKNMYLPHIFNQEGDHPGYS"
BASE COUNT         2337 a         1297 c         1205 g         2146 t
ORIGIN      
        1 aggaactaaa ccaaatcaac cttttttatg acttgagaag ccaaatggat aagctgctac
       61 aggataaaac tcctattgca cgtatggaga tcaagcatat aaaagagatg atttcaaacc
      121 tctttattga taaaagtagc caggaatttc ataaagctct taaagcagag ttccctaagc
      181 ttaccttcga tgatcttagg ctttgctctt acctaaaaca ccgattttta aatgtggaga
      241 tagcagagtt tcttgaaacc tcacccgaag cagtttacac acgtaaatca cggctccgcc
      301 aaaaacttaa ccttgaaaag aatcaggaca ttaatgattt tttatctaat tacacttatt
      361 aatgccttta atatcattcc cttactattt caaaatcacc ggtttcagta attctaatga
      421 tacgtgaagg ccgcataagc ctgatttcgt tttgataaag ttttacaaca taatcaacct
      481 gcgatttcac cttttctgaa atttgttgaa accttattgc acttggttct cctgaaatat
      541 tggccgatgt agatacaatt gctttaccaa acgccgatat cagctcttta caaaactcat
      601 cctttgtaac tcttatacct atagtattat cccctgccgc cactccttta gctacatttt
      661 ttgccccctg ataaataacc gtcaagggtt tatcataact tttaataaga tcaataaata
      721 acgggcttac cttttccaca taatcaaaaa ttcgatccgg cgactccact aacattaaca
      781 gacttttact ttccatacgg cgctttatct tataaatttt atctaccgct ttttggttgg
      841 tagcatcgca gccgattccc cagatcgtgt cagtaggata gaggatagta cctccgctgg
      901 ccagaacttc aacagcattt ttaatctcag tcgttagcaa agggttcatt ttataaaaaa
      961 tataatcaat taaaaccgtg gtttaacacc tttttagcaa tttctcaatt ttaatgtcca
     1021 acgcacattt gaaatggtcc ttagggcatt ttggataacc gtgtaagcca caaggtcggc
     1081 aattaatctt atcatctact tcaataatga cagcgtcatc agataaagga ccaaacccaa
     1141 aagccggtac ggtagaacag aacattgctg ttaccggagc attcatagcg ctggcaaaat
     1201 gcaaaggagc gctgtcgtta acaaaattca tcttcgcatc acgcataagc acagccgatt
     1261 caagaaaaga taatttcccg cacaagttaa tcgaatttgt atgcccagaa ctaacaatta
     1321 tatgttcagc agttgcaaag tcattttttg atcctaaaaa atagatatac tgctccttat
     1381 caacacgttt taaaaaggca atccatttct cagcaggaaa ctgtttagta aaccagagcg
     1441 aagttggtga aatagttaga taaaaatgtg tcttatattg tgacatttta gcgtagttgt
     1501 tcttcgaagg ataaagtctt acagggtaat tatgattatt atcggtaata aaatcaatta
     1561 aagaaagatt tctgctcacc tcgtgcgcac tctttgcagg attggcagat atcctatgtc
     1621 ttacggcttt tgtgaaaaat attgaaaaag gattcttacg aaaacctaac tttatttttg
     1681 cagcggaaaa agcagttatt aggccggtcg aggcaaatcg ctgcaggttt attacaacat
     1741 catattcttc ttttcttatt tgccttatca gtctatatat attttcaaac ttcttattag
     1801 atttatcaaa aacgatcacc ctattcaaca ttggatgacc atttagtaag gattctattc
     1861 cttttttcaa taaaaagtca atttttgctt tcggaaaaaa gtcagataac ttttcaatta
     1921 gtggcgtagc taaagtaaca tcacctattg atgcagtctg aatgatcaga atcctatgaa
     1981 ttattctttt tttcaatatg tttacaaatg attaaaccgg tacatcataa tcacgcagcg
     2041 ctttgttaag cgaggttttt aaatcggtag atggttttct ctttccgatt atcagagcgc
     2101 aggcaacctg atatattccc gatgggaacg attttggaaa agtacctgaa atcaccactg
     2161 atctggcagg aacaaatccc ttatattcga caggcgattc cccggttaca tcaattattc
     2221 ttgttgattg cgtaatgact acatttgctc caattacagc ctctttttca acacggacac
     2281 cttcgacgag gatggatcgc gaccctataa aacaattatc ttcgattatc accggcgatg
     2341 cttgaacggg ttctaacaca cctccaattc ctacgccacc gctaatatga acatttttcc
     2401 cgatttgtgc acacgagcca acggtagccc atgtatcaat catagttcca caatctacat
     2461 aagcgccaat gttaacgtaa gagggcatca tcacaacccc gcgtcctaaa aaggcgccat
     2521 aacgagctac agcatgagga acaacacgaa taccatactt tttgtaattt tgttttagct
     2581 ttatcttatc atggaattca agaatcccgg cctcagtagt ctccatatta cggatgggaa
     2641 aatagagaat aacagccttt tttacccaat cattaacttt ccaacccgaa tcgcttggct
     2701 ctgcgcaacg taatttccct ttatcaagaa gctcaacaac atattcgatg gatttgataa
     2761 cttcggcatc tttaagaaga tcacgatttt tccaagcgct ctctattatt ttaattaacc
     2821 tttccattaa taataaaaat gaaagtttca aaagtaggca aaatattatt atccctatta
     2881 atcaacagaa agccttcctg tgccgagctc ttgatttcaa cagattaacc acggtttaat
     2941 attcgtttgg tttaattctt attgtgttga ttacaaacac ataattggga ttatttctaa
     3001 ttttgtaagc cgaaaactaa aattatgatt taaataaatg ggacgtattc tagcgattga
     3061 ttatggccag aaaagagtag gactggcagt aactgatgaa atgcagatta ttgccaccgg
     3121 attggatact gtccatgtta atgacctgtt tgcctattta gaaaaatata tggagggtaa
     3181 atcaattgaa aaaattgttg ttggcgaacc tcgcaattta gataacactc catcggactc
     3241 acaaagattt attgagccat ttattaaaaa attaaaaaaa cattttcctg acattcctgt
     3301 ttttagagtt gacgaacgct ttacatccaa aatggccaaa gatgttatac ttagctctgg
     3361 cattggaaaa aaagcaaggc aaaagaaaga gttagttgat aaaattagcg caaccattat
     3421 gttgcaatca tttatgcaac aacaattaat gaaggaaaaa tattaaaaca aacaaaatga
     3481 tattacctat tgtcgcgtat ggtcacccgg tcttaaaaaa aatgtcggaa gaaattcaac
     3541 aagatcatcc tgggttaaat gagttgatta acaatatgtt cgaaacaatg tactcctcag
     3601 gaggcattgg gcttgcagcg tcacaggtta accactcgat caggctcttt ataattgacg
     3661 caaacccaat ggcagaggat tttcctgaat taaagggttt taagcaggtg tttatcaatc
     3721 cctatatttt agaggaatca aatgagattg tatctgctga agaaggctgt ttgagtattc
     3781 caaacatacg cgaaagtgtg ccccgcccaa atgagattgt tttagaatac tacgataatg
     3841 actggaattt ggttgaacaa aggttcaaag gttatgcggc aagagttatc cagcatgagt
     3901 atgatcatgt tgaaggcatt ttgttcgttg aaaagattag ccctttgcga aagacattaa
     3961 ttagccggaa gctgaaagag atatcaatag gtaaggtaga tgtagattat aaaatgattt
     4021 ttccgcagaa aaaacgaaac taagatttca atttgttgtt taatcataaa atactaatta
     4081 catgattcga agaagttttt tttacatcac ttttgtggca acgatacttt ttctggccag
     4141 ttgccaaagt tcacgaacga aagccgttaa gctcgttcaa acaatggagg attcggtgtt
     4201 tggctccaca ggcgcaattg acagggtaaa agtaggcgaa ctaattgatt tgtatataaa
     4261 ctttgctgat aagttcagta aggacagcct tgcaccggtt taccttttca aagctgcgga
     4321 catggccatg aacacagggc gatcattgga agctatccaa ctgtatgaga gaatattaga
     4381 taattattcg gaatattcta aagctccaga agccttgttc ttgatgggat acgtgtatga
     4441 aaataacctt ggagagttgg ctaaagctga agagatttac aagaaattct tagagctata
     4501 tcctgataac gacttcgctg atgatgcaga gatatcgttg aaatatttag gtaaaagccc
     4561 tgaagagctc attgaaattt ttcagcaaca gggtgtattt tcaggctttg gagaggagaa
     4621 ttaagtgatt tggagtttta tttctctgtt ttttcttcca agttaaactc actatcaaga
     4681 aagtttttta actgcgatgg tgttatattc aatttgacat tgccacgctg aaaacaagat
     4741 atgtacccag cctcttcgga taccacaata gcaacagcat cggttttttc agtaataccc
     4801 aaggctgctc tatgccttag ccctatctct cccggtaggt tctgatttcc ggatacagga
     4861 agaattaccc gtgctgatct tatcctatta tttaatacga taacagcgcc atcatgtaat
     4921 gggctgtttt tgaaaaacaa gtttaaaata aggtcttcag aaatagcagc atcaattacc
     4981 tgtcctgact cagtatattg ctgcagttcg ttaagccgtg caatcacgat caatgcaccg
     5041 gtttttgtta ccgacatttt cctacacgcc gacacaatag ggtcaacatc aaggataaaa
     5101 atcatttttg ttttaaactt taaaaaacca aaccgtctcc gcatgttaac aagaaaccga
     5161 ggcgtgccaa taagaagcaa aaattgtcgt atttcaggtt gaaaaacaac aattaatgca
     5221 ataaaaccta ctgaaataaa tgctcccaga atttggctaa gcatctccat ctcaagaaga
     5281 agtactacac gccaaaagat gaaaatggcg aaaataccta ttaaaatgtt agttgcagca
     5341 gttcctttca acagattgta aagcatgtaa ataagtactg caaccaatag tatgtctatc
     5401 aggtcagcaa gcttgatctg gataaaagca gttatgaaaa aattagccat aaatagaagt
     5461 taatactatt tgattttcgc tataatttaa aattgatttg acctgccaaa aataatgaaa
     5521 agatgtaacc ttgaataaac atgttgcaaa caattttgct gtttcactgt ttataactaa
     5581 gcatagtgta taaaaagtta tgtcagaaga aaccaaacat aattttcctt tttttcctct
     5641 gaatcttatt gtgatgcccg gcgaaatcca gccacttcat atttttgaac cacgctacaa
     5701 acagcttatc catgatatcc aaacatctgg agaacatttt ggtatcccct ttatgcttaa
     5761 taataaaata tgcaaacttg ggtcgtgcgt aaaattatat aaaatacttt cggaaaccag
     5821 cacaggcgaa atggacatat tggtaaaagg ggtgagcatt atcagtattg aatctatgga
     5881 ggatacattg ggcgaaaaac tatatagcgg cgggtcagtt aaaattttaa aagaattgaa
     5941 caaaacacct tcaaccgagc taagcaacga atttaaaaac taccaggccc aactggcaaa
     6001 aatcaataag atagagggta actctgaaaa aactaacgaa ccattaaacg tattggatat
     6061 tgccggccaa ctaccacttg aaatcgaaga gaaatttaat tttttaatac tgaattgtcc
     6121 gataaaacga gaaacatttc ttttgaacaa gattaagttt ctgcatatga taaatcaaaa
     6181 actggaacag ttaggatata gattcttttt aaattgatga ccagtgcaaa aatcctgata
     6241 aatcccaagt caagcccttt aaaaggatgt tttatctaaa aaacatttca tcgctgttcc
     6301 aagattaaat cgaaaagaaa aggattgaag ttttaaaata aaatgttata gcccaattag
     6361 gatgagatct gattgagcta taacatattt ccagtcagga taaaatcatc agtagatttt
     6421 gttactcaat gatttgtaag ctaagagtag cctggatggt caccttcttg gttaaatata
     6481 tgaggaagat acatgttttt cagtgtaaac ggattcttat ttatcaagtg atatcggatt
     6541 ttgtttattt tggttaataa atcggaaagc agattatcaa ctgattgcaa aatagctttt
     6601 actgattcat ttaatgactt taacaataga tgatccctcc caggatttcc cgaaaggtag
     6661 ttaagagcaa ctgaaaatag ttgaacattt acattacttc ttttagctaa accacaaggt
     6721 atcaaattat tctgcccgct gacaacaacc tcaaatgcaa cttcactaaa cttttgactg
     6781 gttaatatcc ttataaaatt attaagataa acaatgacta gctcgaatat tcttgctgac
     6841 ttttcgaaaa ataccaaata tgtatttgaa actttgcttc tgataatttc tgttttccct
     6901 tcaaagaaat agttagaatt atttgattta gtatttgaga gcgaatgaac cgaagaactt
     6961 ttattattat catcttcaat cataa
//
LOCUS       DAOOAI010000104         6805 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_13266_length_6805_cov_6.234074, whole genome shotgun sequence.
ACCESSION   DAOOAI010000104 DAOOAI010000000
VERSION     DAOOAI010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6805)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6805)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6805
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13266_length_6805_cov_6.234074"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(83..2485)
                     /locus_tag="PK345_07480"
     CDS             complement(83..2485)
                     /locus_tag="PK345_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007366105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPX34893.1"
                     /translation="MKKLWILLFLMLLSVSAGVAQNNFRETVRGVVISGDPAHELIQA
                     SIYVEGSDPGIGTVTDEQGNFTFSVPVGRQKIRFSSIGFFSQEVDVLVNTGKEVVLKV
                     VLEPSFVELQGVEVVVSRDKSRPINALSVTGARTFSTEETFRFAGSLGDPARMVRSFA
                     GVIPANDSRNDIIIRGNSPIGVQWSLDGVEIANLNHFNTGVGMTGGQVTLLNTNLLAN
                     SDFHMSAWPASYGNALAGIFDLKMRRGNNKKHEFWTQVGFNGIELGSEGYFSRKSTSS
                     YLVSYRYSIPDLMEKMGFYKGITPAYQDLTMKFDFDLNENNHLSLIGLWGTSGIEFIT
                     SDVGMVDIDEDILQTMDQRIAINAKTLIFGAAHSVDFTPKTKLTTLFSFVRSDTHMPV
                     DTISLVEPDAEWKTLWDENALEDKYSLHSKLEHRFTYNSLIEAGVKYDLYNVKYLEIE
                     SFTDERGLFTNVDEKGHFSLTRAYAQYRHNMTSKFLLTGGVHGMYLHLNKTYAVEPRF
                     GLRYTPALKHTFALAGGLYSQMIPRSFYFIRTLTPKGDIEYSNKQLGFMKSAHADLSY
                     DWAFAPDWHFKVETYYQWLYDIPVKNDPEATYTMLQTGGAGDNVIMREGNLVNKGTGK
                     NYGVEFTIEKFMSKNYYLLFNSTIYRSTYTNGFNKKEWSTIFDGKFLFNLASGYEMQL
                     KKGWTLFTDAKGSLAGGTRYTPVLEDQSRMEHKVVYDNTRVNELQVRNYFRMDLRIGY
                     RKNRKRFTDEFFIDLQNLTDRKNLYGLFYDIDKGTYNEMLLQGFMSMVTYRINFSISK
                     NK"
     gene            complement(2979..3341)
                     /locus_tag="PK345_07485"
     CDS             complement(2979..3341)
                     /locus_tag="PK345_07485"
                     /inference="COORDINATES: protein motif:HMM:NF025092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase-like regulatory
                     domain-containing protein"
                     /protein_id="HPX34894.1"
                     /translation="MKFIVVLITAVLLSINASAAEGKKESKTTKSNNTEVAYSSIQTV
                     DVKGSVIDGKSRESLAGAAIFIDGAKYYSDLEGNFAISNLKPGKHTLRVELISYQLEE
                     LEVNLEKNQKIDISLIQE"
     gene            complement(3485..3970)
                     /locus_tag="PK345_07490"
     CDS             complement(3485..3970)
                     /locus_tag="PK345_07490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system YwqK family antitoxin"
                     /protein_id="HPX34895.1"
                     /translation="MKHKVTFLSVLLLMVSLVQAQTPKKTPQLYQNEGIYYRDRAQNE
                     LYTGDYREYYDNNTLKLEMQIKNGLPEGTYIIYFENRKPQEIRSYREGKLHGLWRKYD
                     ISGQLISEAEYKNGKKHGTWRIWDELGTQRYEISYNEGNKIGIWRMWDEKGNLIDEKK
                     Y"
     gene            complement(4076..5218)
                     /locus_tag="PK345_07495"
     CDS             complement(4076..5218)
                     /locus_tag="PK345_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005925709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34896.1"
                     /translation="MKKNLFYLFIMSIAIVFASCDKDDPVDPKPVEDKNLAGSITGVR
                     TLDATIEYALVGPLLVEDGGVLNIPAGTTIKAKKGFSSYILVLQGGKININGTADKPV
                     TMTADVANAEQGHWGGLIINGKAPLADPKEKGSTEINSAYLYGGNDPKDNSGSITYLK
                     LEYTGARSSANVEHNGLTLNGVGNGTKIENLFIPNGADDGIEFFGGSVNVKNLLVVNS
                     DDDMFDFTQGYNGTLENCYGIWEVGFVSTESDPRGVEADGNLDGLYPEYPNQSDFKIK
                     NMTIDLRLAPNTTDGYYMHDVIKVRRGAKATIENALVKGQGQVKDLVDLTDGKGAAQE
                     STTISLTNSLTTPFSGKEVNGTGNVTIKAGNTGCPTNIFAWTGYKF"
     gene            complement(5252..>6805)
                     /locus_tag="PK345_07500"
     CDS             complement(5252..>6805)
                     /locus_tag="PK345_07500"
                     /inference="COORDINATES: protein motif:HMM:NF012801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HPX34897.1"
                     /translation="YNFMLVHVNDQYVGEYSGLQTEAFQGSPDLSGIIRRQQTNDNLL
                     IVNQLFSEWKLSRNLKFNLGGTYNRIKGLEPDRRENYFTHLDGVRYNLTRSNRNKRFF
                     SDLKNNDFNVKAALSIKLEDRFYTDNSSIQVGYAGRFVNDKFKAIEYNYSPLGMSDFT
                     MDNLILDQLCEENLKNGLLTMATGETNSYKVLKNTHSGFVEVSYQLAENLAGNAGLRT
                     DIVDLTVNHDVTHAAPGSESIKKTYFLPSLNLKYDINDKNSLRLGISKTYTLPQSKEI
                     SPYQYVNISFASQGNPNIKPSDNYNVDLKWDFYISNSELLSVTTFYKYIQHPIGRVDQ
                     GNSAGLLTYDNIGKGATVGGVELELRKNIFNLYDAQFDKTNRLSFGLNASYIYTDLML
                     DILNTKPRKIQLEGASPFLLNVDLAYNFKKSDKDFITSLVFSYFSDRVHTIGTKGFRD
                     IIEEGVPTLDFVLSYGFTKNFSMSFKAANLLNASYRLSRESSNSEDKTVLNEFKKGQN
                     ISLGVSYQF"
BASE COUNT         1983 a         1457 c         1173 g         2192 t
ORIGIN      
        1 cctttttgcc gtcgaccact ttatctaaga cagcttgaag tgatttttct ttttccgttg
       61 tcattgttta ttcttttaat attcatttgt tcttgcttat agaaaagttg attctatatg
      121 taaccattga cataaagccc tgtaataaca tctcattata ggtgccctta tcaatatcat
      181 aaaagagacc atagagattc tttctatccg tcaggttttg caagtcaata aaaaattcat
      241 ctgtaaacct cttacggttt tttctatacc ctattcttaa gtccattcta aaataattgc
      301 gcacttgcaa ttcattcacg cgggtattgt cgtacaccac tttgtgctcc attctactct
      361 gatcttctaa cacaggagtg tagcgggtac cgcctgctaa cgaccccttt gcatcagtaa
      421 atagcgtcca tcctttttta agttgcattt cgtagcccga agcaagatta aaaaggaatt
      481 ttccatcaaa aatagtactc cactcttttt tattaaatcc gtttgtatat gttgatctgt
      541 agatggtaga attaaacagg aggtagtaat ttttactcat gaacttttca atggtaaatt
      601 ccaccccgta attttttcct gttcctttat ttacaagatt cccttcccgc ataatcacat
      661 tgtcacctgc gccacccgtc tgaagcatcg tataagttgc ctcaggatca tttttcacag
      721 gaatatcata aagccattga taataagttt ccactttgaa atgccaatcg ggagcaaatg
      781 cccaatcgta cgaaaggtcg gcatgtgcgc tcttcataaa tccaagctgc ttgttgctat
      841 actcaatatc gcctttagga gtgagtgttc gaatgaaata aaatgagcgt ggaatcatct
      901 ggctgtatag tccacctgct aatgcaaaag tatgttttaa tgcaggtgta tagcggagac
      961 cgaatcttgg ctcaactgca tacgttttgt ttaaatgaag atacatccca tggactccgc
     1021 ctgtcagtaa aaatttcgat gtcatattat gtctatattg agcataggcc cgtgtgagac
     1081 taaaatgacc tttctcatca acattggtaa agagtcctct ctcatcagtg aatgactcta
     1141 tttcgagata ttttacatta tagagatcat acttcactcc ggcttctatt aacgaattgt
     1201 aggtaaatct atgctccagt ttggagtgaa gcgaatactt atcttccaat gcattttcat
     1261 cccaaagcgt tttccactct gcatcaggct ctacaagaga aattgtatct accggcatat
     1321 gagtatcact tcttacaaaa gaaaatagag tagttagctt tgttttcggt gtaaagtcaa
     1381 cgctgtgcgc agctccaaat attaatgttt tggcgtttat agctattctc tgatccattg
     1441 tctgtaaaat gtcttcatca atatcaacca tacccacatc ggatgtgata aactcaatcc
     1501 cgctagtgcc ccataatcca attaaggaaa gatggttatt ctcattcaaa tcaaaatcga
     1561 atttcatggt aagatcttga tatgccggtg ttataccttt ataaaaaccc atcttctcca
     1621 tcaggtcggg gatagaatat ctatacgaaa caaggtaaga tgaagtgctc ttccttgaaa
     1681 aataaccttc cgatcccaat tcaataccgt taaagcccac ctgtgtccaa aactcatgtt
     1741 tcttattgtt tcccctcctc attttcagat cgaatatgcc tgccagggca ttgccatagg
     1801 aggcgggcca ggcactcata tgaaaatccg aatttgcgag caaatttgta ttaagcaggg
     1861 tcacttgtcc gcctgtcata cccacgccgg tattgaagtg attaagatta gcgatttcca
     1921 caccatctaa cgaccattga acgccgatgg gcgaattgcc tctgattata atatcattac
     1981 gtgaatcgtt agcaggaatc acgcctgcaa agctgcgcac catacgtgcc gggtcaccga
     2041 gcgagccggc aaatctaaaa gtctcttccg tgctgaaagt tcgtgctcct gttactgaga
     2101 gcgcatttat tggtctgctt ttatcgcgac ttactaccac ctcgacacct tgcagttcaa
     2161 caaaagaagg ctccagcacc actttcagta caacttcttt acctgtattt accaaaacat
     2221 caacttcttg cgaaaaaaag cctatactgc tgaatctaat tttttgtctt ccaacaggaa
     2281 cgctgaaagt gaaattacct tgttcatccg ttaccgttcc aattccgggg tcgctcccct
     2341 caacatatat tgacgcctgt atcaactcat gagcagggtc accggaaatc actacgcctc
     2401 ttactgtctc tctaaagtta ttttgtgcaa cacctgccga tactgacaaa agcataagaa
     2461 acaataatat ccacaatttc ttcatgcaaa cattatctat tcgttaaaat actggtataa
     2521 acggttgtgg caacacaaat acaacaacca agcacaacta ccaaaccgaa acgtattatt
     2581 tcctctctta taaaatacat tttcattcat agttatatat ttttgagtcc aagaatttag
     2641 ttatgaatta tggtaaagtt agttaagtcc gtaaagaaaa taattgcgat aaaattaaaa
     2701 tagcattaaa gttttattaa acatttatta agatttgtgt cttggaatga ttgtgctgcg
     2761 actgaggtgc aacaaccaca cgacaattaa tccaaacaca aaagttgagg cggtcatttt
     2821 aatcatgtga ctaagactca cactggcagt gggcattcat ggattatgta accaaaaaat
     2881 aaaaccgtaa ggcaagcctt gccctacggg taaaacagtt aaaaccatca aactcctcag
     2941 caaaaatgga tttaactttt tcttttatta accaactatt attcctgaat caggctaata
     3001 tctatttttt gatttttttc caaatttacc tccaactctt caagctggta tgaaatcaac
     3061 tcaactctaa gagtatgttt gccgggtttt aagttggaaa tagcgaagtt accctctaaa
     3121 tctgagtaat actttgctcc atcgataaaa atggcagctc cagctaaaga ttcccgactt
     3181 tttccatcga ttacggaacc tttaacatca acagtctgta tgctgctata cgcaacttct
     3241 gtgttgttac ttttcgtcgt tttactttct ttcttgcctt cggcagcaga tgcattaatc
     3301 gacaacaaaa cagcggtaat gagaactaca ataaatttca tactcttttt tttaacttaa
     3361 aactaaatgc tcaaaacaaa tttttgaaac cttttacatt acaaagataa accatcaatg
     3421 tgacgaggct attacaataa agttacattt atgttaagtt tttatcagtt tgttatcaaa
     3481 taccctaata ctttttttcg tctattaaat tgcctttttc atcccacatc cgccaaattc
     3541 cgatcttgtt accttcattg taactgattt cgtagcgttg agtccccaac tcgtcccaaa
     3601 tacgccatgt gccgtgtttt tttccatttt tatattctgc ctccgatatg agctgcccgg
     3661 aaatgtcata ttttcgccat aatccatgaa gttttccttc tctgtatgaa cgtatttctt
     3721 gcggcttgcg attttcgaag taaatgatat aggttccctc cggcaaaccg tttttaattt
     3781 gcatttcgag tttgagcgta ttgttgtcgt agtattcgcg ataatctccg gtatagagtt
     3841 cgttttgtgc tctatcgcga tagtaaatac cttcattttg atagagctgt ggagtctttt
     3901 taggagtctg agcttgtact agactcacca tcaataagag caccgataag aaagtaactt
     3961 tatgtttcat ttgatttgtt tttattataa gtgaacaaat ataagcaatt taaaggtatt
     4021 tcggctagtc cgaaatacct tttttgttta taaatcaaat gtgtaatatc aaagcttaaa
     4081 acttatatcc tgtccaagcg aagatatttg taggacatcc tgtattaccg gcttttatgg
     4141 taacatttcc ggtgccgtta acctctttac cgctgaaggg ggttgtcaaa gagttagtta
     4201 aagaaattgt agttgactcc tgtgcagcgc ctttaccgtc agtaaggtcc accagatcct
     4261 taacttgtcc ttgtcccttt accaatgcat tttcgatagt agctttagct cccctacgaa
     4321 ctttaataac atcgtgcata taataaccgt ctgtggtgtt aggcgccaag cgaagatcga
     4381 tagtcatgtt tttgatctta aaatcggact ggttaggata ttcaggatat aatccgtcca
     4441 gattaccgtc agcctctaca ccgcgcgggt cagattcggt actcacaaac cctacttccc
     4501 aaatgccata gcagttttcc aatgtgccgt tgtaaccttg tgtaaagtca aacatatcgt
     4561 catcggagtt taccaccagc aaattcttta cgttaacaga acctccgaaa aactcaattc
     4621 cgtcgtcggc tccgttaggg ataaagaggt tctcgatttt tgtaccgtta cccacaccgt
     4681 ttaatgtaag tccgttatgc tcaacattcg cgcttgaacg agctccggta tattcaagct
     4741 tcaaataagt aatggaacct gaattatcct taggatcatt accaccataa agataggcag
     4801 agttaatttc tgtagaacct ttctctttcg gatcggctaa gggagcttta ccgttaataa
     4861 ttaatccgcc ccagtgtcct tgctctgcat tggcaacgtc ggcagtcatg gtaacgggtt
     4921 tgtcagccgt accgtttata ttgatcttac cgccctgcaa aacgagaata taactactga
     4981 atcccttttt cgctttgata gtagttcctg cgggaatatt caacacgccg ccatcctcaa
     5041 caagaagcgg accaaccagt gcatactcaa tcgttgcgtc aagtgttctg actccggtta
     5101 ttgagccagc caagtttttg tcttccacag gtttcggatc aaccggatca tctttatcgc
     5161 aagaagcaaa aacgattgcg atgctcataa taaacaaata aaataaattc tttttcattg
     5221 tgataataaa ttaaaaaatt agttactcat attaaaattg ataactcact cctaaactga
     5281 tattttgacc ttttttgaac tcattcaata ctgttttgtc ctctgaattg ctgctctcac
     5341 gcgatagacg ataggaagcg ttgagcaaat tagcagcttt aaaactcata gagaagtttt
     5401 tagtgaatcc gtacgataaa acaaaatcca aagtgggaac tccttcttca ataatatctc
     5461 tgaatccctt tgtaccgatg gtgtgcactc tatcgctgaa atagctgaag accagagatg
     5521 taataaaatc tttgtcggat ttcttgaaat tgtaagctaa atccacattc aacaggaaag
     5581 gagatgctcc ttcaagttga atcttacgtg gtttcgtgtt aaggatatct agcatcaaat
     5641 cggtatagat ataggaagca ttcaatccga aagagagacg atttgttttg tcgaattgag
     5701 catcgtaaag gttgaaaatg tttttacgaa gctccaattc aactccacct acagtagcgc
     5761 ccttgcctat attgtcatag gtaagaagtc ccgcagagtt tccttgatcc acacgtccga
     5821 taggatgttg tatgtacttg taaaaagtag taaccgaaag cagttcgcta ttggatatgt
     5881 agaaatccca ctttaaatcc acattataat tgtccgacgg cttaatgttc ggatttccct
     5941 gactcgcaaa agagatgttt acgtattgat agggcgagat ctctttcgat tgtggcaaag
     6001 tgtatgtttt acttattcct aacctcaagc tatttttatc atttatgtcg tatttcaagt
     6061 ttaaactggg taagaaatag gttttcttta tcgattcgct tcccggagcg gcatgagtta
     6121 catcgtgatt aaccgttaag tcaacaatgt ccgttctcaa tccggcatta cctgccaaat
     6181 tttcagccaa ctggtacgat acttccacaa aaccggaatg tgtgttttta agaactttat
     6241 aagagtttgt ttctcccgtt gccattgtca gcaaaccatt ttttaaattc tcctcacaca
     6301 attgatccaa aatcagatta tccattgtga aatcggacat tcctaacggg ctataattat
     6361 attcaatcgc tttaaactta tcattgacaa acctccccgc atatcccact tgtatggatg
     6421 aattatcggt ataaaatcta tcctccaatt ttatcgataa cgctgcttta acgttaaagt
     6481 cattgttctt taaatcggag aaaaagcgct tattgcggtt actgcgggtt aaattgtacc
     6541 gaactccatc aagatgtgta aaatagtttt cccggcgatc gggttccaat ccttttatcc
     6601 tattatacgt tccgcctaga ttgaatttga gattacggga taatttccac tcggaaaaca
     6661 attgatttac aatcaaaaga ttatcgttgg tctgttgcct tctgataata cctgacaggt
     6721 caggactacc ctgaaatgct tcagtctgca aaccactata ttctcccaca tattggtcat
     6781 taacgtgaac gagcatgaag ttata
//
LOCUS       DAOOAI010000105         6600 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_13746_length_6600_cov_5.090909, whole genome shotgun sequence.
ACCESSION   DAOOAI010000105 DAOOAI010000000
VERSION     DAOOAI010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6600)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6600)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6600
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13746_length_6600_cov_5.090909"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(500..3124)
                     /gene="alaS"
                     /locus_tag="PK345_07505"
     CDS             complement(500..3124)
                     /gene="alaS"
                     /locus_tag="PK345_07505"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019244889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HPX34898.1"
                     /translation="MMNSAQIRHTFLKFFENKRHKVLPSAPMVLKNDPTLMFTNAGMN
                     QFKDIFLGNSPVDHPRVVNSQKCLRVSGKHNDLEEVGHDTYHHTMFEMLGNWSFGDYF
                     KKEAIHWGWELLTEVYGLDKERLYVTVFGGDSAEGLDADQEAYQVWRDILADDKILYG
                     SKKDNFWEMGDTGPCGPCSEIHIDLRSEEEIKLKPGREMINTGHPEVIELWNLVFIQF
                     NRKNDGKLEPLPAKHVDTGMGFERLAMALQKKKSNYDTDIFQPIINETALISGKRYGD
                     DAKQDVAMRVIADHLRAIAFAIADGQLPSNNKAGYVIRRILRRAVRYGYTFLDIKEPF
                     IHKLVAILVREMGDFFPELKAQQDLITKVIAEEEQSFLRTLSHGIKRFENYLSTNANL
                     VSIDGDFAFELFDTYGFPVDLTQLMAREHGLDVDMQAFQANLDQQKQRSRLAAEVDTD
                     EWVEIRKGDDQTIFLGYETLDAEAQILKFRKVKTKKESFYQIVLDQTPFYAESGGQIG
                     DRGALVSAEGSFFIRDTQKENDLIVHIADKLPKDLFVTFSAKVDAEKRRMTMNHHSAT
                     HLMHAALKVVLGDHVEQRGSLVDENHLRFDFSHFAKMTAEQITQVEQIVNAKIRENIP
                     VTDQRNVPIDEAKAMGAAALFGEKYGEHVRVISFDPKFSIEFCGGTHVPATGQIGMFK
                     IVSETAIAAGIRRIVAITADKVEELMIQQDIIISNLKEIFKNKTDIVKAVNELVEEKK
                     MLEKQIELMKREKIANLKSKLSKMSEGIKGINFIAYRFEGDTQGAKDLAFMMKDIVDN
                     LCLVLGYEKEAKAGLTVMLSDSLVKSYGLNAGEIVRELAKEIGGSGGGQPHFATAGGK
                     NPKGIEKALKKAKEIIEG"
     gene            3240..4211
                     /locus_tag="PK345_07510"
     CDS             3240..4211
                     /locus_tag="PK345_07510"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013546849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HPX34899.1"
                     /translation="MRKTKYHFNPITITFEIVKYPFQKKVRRILLYFLAAALYAVVVL
                     AVAYSYHDSPKERYLKRELTNMEFKYQILNDRLDKMGLVIQDLQERDDNIYRVIFEAE
                     PIPTSIRKAGFGGVDRYADLDGYLYSGLLKSTSEKLDIMAGQLVVQSKSFDEVFEMAK
                     NKAQFLASIPAIQPVDNKDLRRISSYFGYRMDPFYKVMKMHDGVDFSAPIGTKIYATG
                     DGTVSFTEKGKNLYGNNLYIDHGFGYGSFYAHCKDIKVKTGQKVKRGEVIATVGNTGK
                     STGPHLHYEVRKNGRSVNPINFFFNDISPEDYQRMIEYSSRPSQSMD"
     gene            4274..4609
                     /locus_tag="PK345_07515"
     CDS             4274..4609
                     /locus_tag="PK345_07515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HPX34900.1"
                     /translation="MYYKIGEVAKMFHVNTSLIRFWEKEFDIIKPKKNKKGNRLFTPE
                     DVENFRIIYHLVKERGFTLKGAKEKLNQNRDKTIENAEIVISLQKIKTFLLDLKEQLD
                     NLPVLTDQE"
     gene            complement(4735..5970)
                     /locus_tag="PK345_07520"
     CDS             complement(4735..5970)
                     /locus_tag="PK345_07520"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004327847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HPX34901.1"
                     /translation="MKPTIAILAGGGPAPGINTVIGTIAKVFLTDGYRVLGLHGGFKS
                     LFIQNPEIDEINFEHADKIYKQGGSAIRMSRYKPKDEEFTVDFFIRENVKLLVTIGGD
                     DTASTANRISKFLAEHDVKIQNIHVPKTIDNDLPLPEGSPTFGYQSAKQEGVRIAQTI
                     YEDARTSGNWFVVAAMGREAGHLAFGIGAACHYPMIIIPEMFNKTELTIHKIVKLSIS
                     SILKRKLLGITYGAVIISEGVFHFMSDDEIKSTNINFTYDEHGHPELGNVSKAHIFNI
                     LIQRELKRLELNVKSRPVEIGYEIRCVQPIAFDLLYCALLGMGVKKLFDQGVSGCMVT
                     SDPIGDIAPLFLKDVEDEKGKVKPRLVNINSQKARLVYGFNHQYITEEDYEAASKYVE
                     NPEEFDFYKILNWDKIELI"
     gene            complement(6144..>6600)
                     /locus_tag="PK345_07525"
     CDS             complement(6144..>6600)
                     /locus_tag="PK345_07525"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HPX34902.1"
                     /translation="DRNTLYASLLAILAAFSFGSSTVFSKMILGRYNFVTSTFYRFGF
                     TAFIMFIIMLGFGDFGQINATTRENWFFIALICLTTGSGTMFLYYYGLRHVKATTATI
                     SELMFPVSAVFFDYVFNKSILSPIQWVSAAVIIYSIFKINTAAERELKS"
BASE COUNT         2119 a         1437 c         1094 g         1950 t
ORIGIN      
        1 acaatactaa agcaataagt attacaaaac taaattgctt tttaaaagat agaaataatc
       61 tcattgtctt aattgttttg attaataata aaagtttata actcaagatt ttgaatcctt
      121 caattttttt tggttgtaaa agtaaatcaa attacattaa taaaatctta aattttatta
      181 atatttatga accattgaat aagtcagggt ttttgtgaaa aaaaattttt ttttgaccgg
      241 ttgagagcat aaaaataacc tacccaactg tttttgaaca atttcgcaac tcacggcatc
      301 aaaatcttct accccacgcc aatagcgcaa tgtagtgaat aatgaacgat caaagcatct
      361 ttataggtgt attgacacaa tccgagaaaa atcagtttat tatagatttt tcaccaaatc
      421 cttaatacag atcaagtact tatatatact atagaaaaaa atgggtataa taccccgaat
      481 actaacaaat gaaattccac tacccttcta taatttcttt agccttttta agcgcttttt
      541 caataccttt agggtttttg ccgccagccg ttgcaaagtg cggttgcccg ccgccacttc
      601 ccccaatttc tttagccaac tccctcacta tctcaccggc attcaagcca tagcttttca
      661 ccaagctatc cgaaagcatt actgttaatc cggctttggc ctctttttca tatcctagca
      721 ccaaacagag attgtccact atatctttca tcataaaagc aaggtctttt gcaccctgag
      781 tatcgccttc gaacctatat gcaataaaat tgatcccttt gataccttcc gacatcttac
      841 ttagcttaga tttcaaatta gctatcttct cacgtttcat cagctcaatc tgtttctcaa
      901 gcatcttctt ctcttctacc aactcattga cagcttttac gatgtcagtt ttatttttaa
      961 atatctcctt gaggttcgaa attatgatat cctgttgaat catcagctcc tccaccttat
     1021 ctgccgtaat agcaacgata cggcgaatcc cggcagcaat tgcggtttct gaaacgattt
     1081 taaacatacc tatttgtcct gtagcaggca catgtgttcc tccacaaaac tctattgaaa
     1141 acttgggatc aaaagatatt actctaacgt gttcgccata tttttcacca aacaaagctg
     1201 cagctcccat tgcctttgcc tcatctattg gcacgtttcg ttgatcggta acaggtatat
     1261 tttcacgtat tttagcattc actatttgct ccacctgtgt aatctgttcg gcagtcatct
     1321 tggcaaagtg agaaaaatca aaacgcagat gattttcatc tacaagagaa ccacgttgtt
     1381 ctacatgatc gcctaatacc actttcagag cagcgtgcat aagatgcgta gcgctatgat
     1441 gattcattgt catacgccgt ttttcagcat caactttagc agaaaaggtt acgaaaagat
     1501 cttttggcag tttatcagca atatggacaa taagatcatt ctctttttgt gtatctctta
     1561 taaaaaagct accctctgcc gatacaagcg ctcctctatc gccgatttgc ccccctgatt
     1621 cagcataaaa cggcgtctga tcaagaacga tctgataaaa gctctctttc tttgttttaa
     1681 cctttctaaa tttcaaaatc tgtgcttcag catctaaagt ttcataacca agaaaaatgg
     1741 tttggtcatc tccttttcta atctccaccc actcatcggt atcaacctct gccgcaagtc
     1801 gcgaacgctg tttctgctga tctaaattgg cctgaaaagc ctgcatatcc acatccaaac
     1861 catgctcacg agccataagt tgagtcagat caacggggaa accgtaagta tcgaaaagct
     1921 caaaagcaaa atcaccatct atcgaaacaa gattggcgtt cgtggaaaga taattttcaa
     1981 accgctttat tccatgcgac aatgtacgta agaaggattg ctcctcttct gcaataacct
     2041 tcgtaatcaa atcctgttga gctttaagct caggaaaaaa atcccccatt tccctcacta
     2101 atattgccac caacttatgt ataaaaggct ctttaatatc gagaaatgta tagccataac
     2161 gaacagcccg tcgaaggatc ctccttatta cataacccgc tttattgtta gaaggcaatt
     2221 ggccgtcagc aatagcgaaa gcaatagccc gcaggtgatc ggcaattacg cgcatggcga
     2281 cgtcttgttt agcatcatcg ccataacgtt tacccgaaat gagagccgtt tcgttgataa
     2341 taggctgaaa aatatcagta tcataattcg atttcttttt ctgaagcgcc attgccaacc
     2401 gctcaaatcc catccccgta tctacatgtt tagccggtaa aggctctaac ttaccatcat
     2461 ttttacggtt aaactgaatg aaaacaagat tccaaagctc aatgacttca ggatggccgg
     2521 tattgatcat ttctctacca ggtttcagct ttatctcctc ctcgcttctc aagtcaatat
     2581 gtatttcgga acacgggcca caaggtcctg tatcgcccat ctcccagaag ttatcctttt
     2641 ttgatccata caaaatctta tcatccgcca aaatatcacg ccaaacctga tatgcctcct
     2701 gatcggcatc aagcccttcg gcgctgtcgc ctccaaaaac agtgacataa agtctctcct
     2761 tatcaagccc atatacctcg gtaagcaatt cccaacccca gtgaatcgcc tcttttttga
     2821 aataatcccc aaacgaccag ttccccaaca tctcaaacat agtatgatga taagtatcat
     2881 ggccaacctc ctccaaatca ttatgcttac ccgaaacgcg aagacacttc tggctgttta
     2941 ctacacgggg atgatctacc ggcgaatttc ccaaaaagat atctttgaac tgattcatac
     3001 cggcattggt aaacataaga gtaggatcat tttttaacac catcggcgcc gaaggaagta
     3061 ccttatggcg cttattttca aagaatttaa gaaaagtatg acggatttgt gctgaattca
     3121 taatacaata aataaaaata acaactgttt atcattctat aaaaaagata caaaattagt
     3181 ttttttgcct aattttgcac acgttataat ctaatcaatc ttttttgtaa acgactggca
     3241 tgagaaaaac caaatatcat ttcaatccaa taactataac atttgaaatc gtaaaatatc
     3301 cttttcagaa gaaggttcga aggatactgt tatatttttt ggcagcggcc ctttatgcag
     3361 ttgttgtatt agctgtagct tacagctatc acgattcccc caaagaacgt tacctcaaac
     3421 gggaactcac aaatatggaa tttaagtacc agatactaaa cgaccgcctt gataaaatgg
     3481 gcttggtaat tcaagacctg caagaacgag atgataatat ttaccgtgta attttcgaag
     3541 ccgaaccgat acccacatca ataaggaaag ccggctttgg tggcgttgac cgatacgctg
     3601 acctcgatgg atatctttac tcaggattgc tgaaaagcac cagcgagaaa ttggatatta
     3661 tggcaggtca attggtggta caatcaaaat cgtttgatga agtttttgaa atggcaaaaa
     3721 acaaagctca gtttcttgca tctatacctg ctattcaacc tgttgacaat aaagacctaa
     3781 gacgtatttc atcatacttt ggatatagaa tggacccgtt ttataaagtg atgaaaatgc
     3841 acgatggcgt tgacttctcg gccccgatag gaacaaaaat ttatgccacc ggcgatggaa
     3901 ctgtaagttt tacagaaaaa ggaaaaaacc tttacggtaa caacttatat attgaccacg
     3961 gtttcggata cgggagtttt tatgctcatt gcaaagatat taaagtaaaa accggccaaa
     4021 aggtaaaacg tggggaagtg attgctaccg ttggaaacac aggaaaatct accggcccac
     4081 atctccatta tgaagtaaga aaaaatggcc gctcagtaaa cccaattaat ttctttttca
     4141 acgacattag ccccgaggat taccagcgaa tgattgaata ctcttcaaga ccatcgcaat
     4201 caatggatta atttgaataa caaactctgc ctattactat attaccatgt ctgcatttcc
     4261 caaaaccgaa aaaatgtatt ataagatcgg cgaggttgcc aaaatgtttc atgtaaacac
     4321 atcactaata agattctggg aaaaagagtt tgacattata aaaccaaaaa aaaataaaaa
     4381 ggggaatcgc ctcttcaccc ctgaggatgt ggaaaacttc cgaattattt atcatcttgt
     4441 taaagagcgt ggctttacac ttaaaggcgc taaagagaaa ctcaaccaaa acagggataa
     4501 aacgatagaa aatgcagaaa tcgtaatatc attgcaaaag atcaaaacat ttttactcga
     4561 tctgaaagag caattggata atctacctgt actcaccgat caggaatgac aggatataaa
     4621 gcttaagttt ttccgctttc cgctattagg aatcctacct actttgctct ctctttagtt
     4681 ttaacaaatc agttaaatac taactaccat agtattgata gactatgctg atagttaaat
     4741 cagttcaatt ttgtcccaat tcaatatttt gtaaaaatca aactcttcag gattttcgac
     4801 atacttggat gcagcttcat aatcttcttc tgtaatgtat tgatgattaa agccataaac
     4861 caatcgtgct ttttgcgaat tgatatttac aaggcgtggt tttacttttc ccttttcatc
     4921 ttccacatct ttcaaaaaaa gtggagcaat atctccgata ggatccgaag taaccataca
     4981 tccggaaact ccttggtcaa aaagcttttt cactcccata cccaacaatg cgcaatagag
     5041 cagatcaaaa gcaattggct gtacacaacg gatttcgtat ccaatctcca ctgggcggct
     5101 tttcacattc aattccagtc gtttcaactc gcgctgaata agaatattaa agatatgagc
     5161 cttgctcaca tttccaagtt ccgggtggcc atgttcgtca taggtgaaat ttatattggt
     5221 agatttaatc tcgtcatccg acataaaatg aaaaactcct tcactgataa ttactgcccc
     5281 ataagtgata ccgagcagct tacgtttcaa tattgatgat atgctaagct ttactatttt
     5341 atggatagtg agttcggttt tattgaacat ttccggaata attatcatag gataatggca
     5401 agcagcgcct attccaaaag cgagatggcc ggcttcgcgc cccattgccg caacaacaaa
     5461 ccaattacca ctggtacgag cgtcctcata aatcgtttgg gcaatcctaa cgccctcctg
     5521 cttagccgat tgatagccaa aagtaggtga accttcgggc aaaggcagat cgttatctat
     5581 tgtttttggc acatggatat tttgaatttt cacatcgtgt tcggctaaaa attttgatat
     5641 ccgattggcg gtagatgctg tatcatcgcc acctatagta acaagtagct ttacattttc
     5701 tctgataaaa aagtcaacgg taaattcttc gtctttcggt ttatatctgc tcatccttat
     5761 ggctgagcct ccttgcttgt aaattttatc ggcatgctca aaattgatct catcaatctc
     5821 aggattttgt ataaaaagac tcttaaaacc gccatgcaac cccaaaaccc tgtatccatc
     5881 cgtcaaaaag actttagcaa ttgtaccgat aacggtatta atacccggag ccggccctcc
     5941 accggcaagt atagcaatag taggtttcat actttatttt aattaaataa taacgaagca
     6001 aaggtaacct atttttgatg atttaaaact aagcttaaat tcaacccata ttaatctgct
     6061 catcctaagg agattaatat ttcaataaaa aaaacaaaag ctaacttgat tagataaaat
     6121 aactcctcgg ctctataaac cttttatgat ttaagctccc tttcggcggc agtattgatc
     6181 ttaaaaatgg aataaattat tactgcggcg ctaacccatt gtattggcga aagaatgctt
     6241 ttattgaaaa cataatcgaa aaagactgca gaaaccggaa acatcagctc gctgattgta
     6301 gccgtagtgg cttttacatg tcgcaggcca taataatata aaaacatcgt tcccgagccg
     6361 gttgtcagac agatcaaggc aataaaaaac caattctcgc gcgtggtagc attgatctgc
     6421 ccaaaatcac caaatcccaa catgataata aacataataa atgcagtgaa gccaaaacga
     6481 taaaaggtgg aagtaacaaa gttgtagcgg cccaatatca ttttggaaaa aaccgtagag
     6541 ctaccaaacg aaaatgcagc aagaatagca agaagtgaag catatagagt atttcgatca
//
LOCUS       DAOOAI010000106         6557 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_13839_length_6557_cov_5.254229, whole genome shotgun sequence.
ACCESSION   DAOOAI010000106 DAOOAI010000000
VERSION     DAOOAI010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6557)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6557)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6557
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13839_length_6557_cov_5.254229"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            287..1723
                     /locus_tag="PK345_07530"
     CDS             287..1723
                     /locus_tag="PK345_07530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34903.1"
                     /translation="MNYLLRYTQLLSIAFIIISNSALSSNGKSATTLPSGWEFVPTSS
                     SHVIAITTDVAYNCIDLSVGDYIGVFYTNDQGGLSCGGAVEWQSGQNFVVVAYGDDIT
                     TIDIKDGFSENEVITWKVFYSQSATEQLIYVDYDSSMPNFNGLFCTNGISMLVSVLNP
                     MEIMANAVPNEVCFGDEVQLSVNISGGCGNVEYSWSSDPVGFVSSQPNPLVAPLDNTT
                     YFVTVSNSFGDTKTDEVTVTVKSLPQIFCSEDFEVCLGSESLLLDVVYPQGGVYSGDG
                     VVFENGAYYFEPWNGIGDFLVSYCYTDNSTGCSDCCEFIISVKADQLIEIPTGWSGIS
                     SNITPENSSLPELLYPITYPLIILYNFDGIYWPGGNTFTIVNWNVSSGYVIKSEWDTT
                     LPICGTTIQNSIVQLNEGWNMIPVYSFNNIDVELLFSNVEGFEIATDVAGLGVYWPEY
                     GINTLGAVKSGKAYLVKTNSSGAISFSR"
     gene            1891..2814
                     /locus_tag="PK345_07535"
     CDS             1891..2814
                     /locus_tag="PK345_07535"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HPX34904.1"
                     /translation="MIEVKSLTKSYNGRKALKGINFTIQTGELYGLLGPNGAGKTTII
                     SIMSSILDADSGEVTIGRYNLSTDAKSVKKVIGVVPQEIALYNELTAYENLLFWGGLY
                     QVPGKELSNRANELLNLFGLEDRKNDKVGNYSGGMKRRINIAAALLHRPKVLFMDEPT
                     VGIDPQSRNLIFEMIEKLHSQGMTIVYTTHYMEEAERFCNRIGIIDNGQIIAEGTLDE
                     LKSTISTKEIINITFSNINEEKANEIKSFWSDAKQSDTSILFKSSNVKFDLSKLVLKC
                     NQIGLDITNVEIMKVNLETIFLNLTGKQLRD"
     gene            2816..4087
                     /locus_tag="PK345_07540"
     CDS             2816..4087
                     /locus_tag="PK345_07540"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX34905.1"
                     /translation="MIRLAVKDLRLFFRDRRSMILTFAIPIVLMTIFAFAFGGVGKTR
                     QSNKITLLVSDLDNTPLSKKAILQLDSLKSIQLQAVTLEEAEESIKKGNRSCVLVIHI
                     GFSDSLIKGAELPLELRYDEARGIEVGLIQQSLVPTIAMLPFNLLNSKQMIGNRLARM
                     AGINDPKAKEAIKTQSDHLYDAISDGILRSGKRDKVNIASNFMGGDIKMTKLVRSTND
                     NQLGLIQAVAGTAVMWLLFSVVGVGMSILDEKHEGTLTRLLYTPTNPVNILFSKMISA
                     NVISILQLLIMFLFASLAFGLEIYSNLGGMLINIIATAFACSSFGILLASLAKNRSQV
                     QGLSILIILVMSAIGGSMIPLIFMPSFMQKIAVVSVNYWSIQGFYDIFWRNFSIFNPT
                     FISRISVLILIGIILNILAVIMFKKNILKLT"
     gene            complement(4301..4549)
                     /locus_tag="PK345_07545"
     CDS             complement(4301..4549)
                     /locus_tag="PK345_07545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34906.1"
                     /translation="MNTIRILGIYAGEKVSDKAALQKVLTNYGNIIRTRLGLNQEEGK
                     YGIIILELAGDEQEMLYFENALLKIEGLEIQKMQFIDS"
     gene            complement(4561..4818)
                     /locus_tag="PK345_07550"
     CDS             complement(4561..4818)
                     /locus_tag="PK345_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34907.1"
                     /translation="MKKTKIIGILIRDRIKEAGKLQILLSSHNEIIRSRLGFHELSQE
                     VCSRVGIIILTLDGNPDEWKPFEKELSAIEGIEFKEMLFNY"
     gene            complement(4828..6255)
                     /gene="hydG"
                     /locus_tag="PK345_07555"
     CDS             complement(4828..6255)
                     /gene="hydG"
                     /locus_tag="PK345_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019541400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[FeFe] hydrogenase H-cluster radical SAM
                     maturase HydG"
                     /protein_id="HPX34908.1"
                     /translation="MKFSPEKCTIPDRRMEPFIDPAELWGFLNNTKPTKERVKEIIAK
                     SLEKQRLTLEETAALINADNPELVEEIKEGARTLKKKVYGNRIVLFAPLYIGNHCVNN
                     CKYCGFKASNRFAIRRKLTDEEIIRETEALEENGQKRLILVYGEHPMYSPEYIAHTVK
                     LVYGVKKGNGEIRRVNINAAPLDIEGFRTVKNAGIGTYQIFQETYHPEMYKFYHPSGP
                     KRDFNYRLTSLDRAQEAGLDDVGIGALFGLYDWRFEVMGLVRHTNHLEAVYNVGPHTI
                     SFPRIKDASEFNIDLKYMVNDKDFAKLVAILRLAVPYTGMILTAREAPEVRSEVMQYG
                     VSQIDGGTKLEIGNYSSTLNEEQNLNREQFRVNDARSLSDVIEELLDKNYLPSFCTAC
                     YRHGRTGEHFMEFSVPGFIKRFCTPNAILTLAEYIMDYANPKVAEKGWKVIEDNMAEL
                     HNEKQVIEVRKRIERIKQGERDLYF"
     gene            complement(6339..>6557)
                     /locus_tag="PK345_07560"
     CDS             complement(6339..>6557)
                     /locus_tag="PK345_07560"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HPX34909.1"
                     /translation="IKVKDSGIGINEENLQELFKDFMRIKSDQTRNISGSGLGLSIVK
                     KIVDLNNGEISVESKLNEGSTFKVVLPV"
BASE COUNT         1961 a         1159 c         1202 g         2235 t
ORIGIN      
        1 ttttttatca acagtttatt tttgataaac aggacttaaa catgttttta aggatcgact
       61 aattaattta atcaatagta atccttttag ctccgtctat tgttattaat tgttcgattt
      121 cgatttagtg tttctttagt acaattctta ttctaattag gataaattaa atttttctag
      181 aaatagtcgg tattaataat tacttgtaat tttacactcg gatttatcaa acaaggattg
      241 aactttttaa taggaaactg attgcgaaaa attaaaacta aaaactatga actatcttct
      301 acgttatact caacttttat ctattgcttt cattatcatt tcaaatagcg ccctgagttc
      361 aaatggtaaa tctgccacca ccttaccttc aggatgggag ttcgttccta ccagttcttc
      421 gcatgttatt gctataacca ctgatgttgc ttacaattgt attgatcttt cggtaggtga
      481 ttacattggt gttttttata caaatgacca aggaggatta tcatgtggcg gagctgtaga
      541 atggcaatcc ggacaaaatt ttgtggtagt agcctatggc gatgatataa caacaattga
      601 tattaaagat ggcttttcgg aaaacgaagt aataacatgg aaagtttttt attcgcaatc
      661 agcaaccgaa caacttatct atgtggatta tgattcatca atgcctaatt ttaacggatt
      721 attttgtaca aatgggattt cgatgctggt tagtgttctt aaccctatgg aaataatggc
      781 taatgccgtg cccaacgagg tctgctttgg tgatgaggtt cagttgtcag taaatatctc
      841 tggaggatgt ggtaacgttg agtattcttg gtcttcagac cctgtcggct ttgtctcttc
      901 tcaaccaaat ccattagtag ctccgttaga taatacaact tattttgtta cagtcagcaa
      961 ctcattcggt gatactaaaa cagatgaagt aactgtaacg gttaaatctt tgccacaaat
     1021 attttgttct gaggattttg aagtttgcct tggctcggaa agtcttttac tcgatgttgt
     1081 gtatccgcaa ggtggcgtat atagtgggga tggggtagta ttcgaaaatg gagcttatta
     1141 ttttgagcct tggaatggca ttggagattt tttagttagc tattgttata cagataactc
     1201 aaccggatgt tctgattgtt gtgagtttat aattagtgta aaagctgatc aattaattga
     1261 aataccgaca ggctggagtg ggatatcaag taatattacc cccgaaaatt cttctttacc
     1321 agagctgctg tacccaataa cttatccatt aattatactt tataattttg acggaatata
     1381 ttggccggga ggcaatactt ttacaatagt taactggaac gtaagctctg gttacgttat
     1441 taaatccgaa tgggatacta ctctgcctat ttgcggcaca actattcaaa attcaatagt
     1501 acagcttaac gaaggttgga acatgattcc tgtttattcc tttaataata ttgatgttga
     1561 actgcttttt tctaacgtag aaggatttga gattgctacc gatgtcgccg gcttaggtgt
     1621 ctattggcct gaatacggaa ttaataccct tggcgctgtt aaatccggaa aagcctattt
     1681 agtaaaaact aacagctccg gcgccataag tttttctcgt tagttttatt tattaagccc
     1741 gctccgagag gtaagaattt cagatgttca tgtatttgtg gttaacaata ctacccggat
     1801 ggatttcatc tattataata caaaattttt attgctttgt gtagcttggt tattggcaat
     1861 attgcaaaaa cagcgctata aagaaaaaat gtgatagaag taaaaagttt aacaaagtcc
     1921 tacaatggac gtaaagctct aaaaggaata aactttacca tacaaaccgg tgagttatat
     1981 ggattattag gccctaatgg agcagggaaa acaactatca tttccataat gagctcaata
     2041 cttgatgctg attctggaga ggttacaata ggtcgataca atttatcaac agatgccaaa
     2101 agtgtaaaaa aagttattgg cgttgtgccg caggaaattg cattgtacaa tgaactcacc
     2161 gcatatgaga atctattatt ttggggtggt ttataccaag ttccaggcaa ggaattatcg
     2221 aatcgggcaa atgaactgct gaatttattc ggtcttgaag ataggaagaa cgacaaagtc
     2281 gggaactatt ccggcggtat gaaacgacga attaatattg cggcagcgct gttgcaccgc
     2341 cctaaggtac tttttatgga tgagcctacg gtgggtattg acccgcaaag tagaaacttg
     2401 atttttgaaa tgatagaaaa gttgcatagc caggggatga ctattgttta tactactcat
     2461 tatatggaag aggcagaaag attttgtaac cgtatcggta ttattgataa tggacaaatt
     2521 attgctgaag ggactttgga tgaattaaaa agcacaattt caacaaaaga aatcattaat
     2581 attacttttt caaatattaa tgaggagaaa gctaatgaga ttaaatcgtt ttggagcgat
     2641 gcaaagcaat cagatacgtc cattcttttc aaatcatcaa atgtaaaatt tgatttatca
     2701 aaattggttt taaaatgtaa tcaaataggg ttagatatta cgaatgtcga aattatgaaa
     2761 gttaaccttg aaacgatatt tttaaatcta accggaaaac aattgagaga ttaaaatgat
     2821 aagattagcc gtaaaagact taagactttt ttttagggac aggcgttcga tgatactcac
     2881 ttttgcaatt ccaattgttt tgatgacgat ttttgcattt gcatttgggg gtgtcggtaa
     2941 gacaagacag agcaataaaa ttactttatt ggtcagcgat ttagataata cacctctatc
     3001 taaaaaagca attttacaat tagattcctt aaaatccatt cagcttcaag ctgttacgtt
     3061 ggaggaggca gaagagagta ttaaaaaagg aaatagaagt tgcgttttgg ttattcatat
     3121 tggtttttcc gattctttga taaaaggagc tgaattaccg ttggaattaa gatatgacga
     3181 ggcgcgagga atagaagttg gtcttataca acaatcactc gttcccacta ttgcgatgct
     3241 accttttaat ttgctaaact caaagcaaat gataggcaat agattagcaa gaatggctgg
     3301 cattaatgat ccgaaagcaa aggaggctat caaaacacag tccgatcatc tttacgatgc
     3361 tatttcagat ggcattttga gaagcggcaa aagagataaa gttaatatag cctcaaactt
     3421 tatgggtggc gatataaaaa tgaccaagtt ggttagatct actaacgata atcaattagg
     3481 tttgattcag gctgttgcag gcacggcggt aatgtggttg ttattttcag tagttggtgt
     3541 tggaatgagt attttggacg aaaaacatga aggtacatta actcgcctgc tctatacccc
     3601 aacaaatccg gtaaatattt tgtttagcaa aatgatctca gcaaatgtta tctctatttt
     3661 acaattgttg ataatgtttc tgtttgcaag tttagctttt ggcttagaaa tttattctaa
     3721 tttaggtgga atgctgatta atatcattgc aacagctttt gcctgttcct cttttgggat
     3781 tttgctagct tctcttgcaa aaaacagatc tcaagtacaa gggctttcaa tattgataat
     3841 tttagtcatg agcgccattg gtggcagtat gattccttta atctttatgc catcttttat
     3901 gcaaaaaatt gctgttgtat cagttaatta ttggagtatt caggggtttt acgatatatt
     3961 ttggaggaac ttctcaattt ttaatccgac ctttatttcg agaatctctg ttcttatttt
     4021 gattggaata attttgaata ttttggcggt aataatgttc aaaaagaaca ttttaaaact
     4081 gacttgaccc aattgttaat gataaattga acgttttaaa taacagtact ttacgagtgg
     4141 catttttgcc gttgaggcaa accattttct tgttttccgc attggaacac cctaaaattc
     4201 aggaaaacag gataacacct attgttacga catccaaaac ttgactatcg ttaaaagttt
     4261 cgatgttatt tttctttctt ttcaggttaa ccaagtagca ttaactatct ataaactgca
     4321 ttttttgaat ttcaagccct tcaattttca aaagtgcgtt ttcaaaatac agcatctctt
     4381 gttcatcgcc tgcaagctcg aggatgataa taccgtattt accctcctcc tgatttagcc
     4441 caagccgggt gcgaataata tttccatagt tagttaaaac cttttgcagt gcagctttat
     4501 cgctaacctt ttcaccggca tatataccta aaattctgat ggtattcatt tttcttcttt
     4561 tcaataatta aacaacattt ccttaaactc gatgccctca atggccgaga gctctttttc
     4621 aaacggtttc cattcgtctg gatttccgtc cagggtaagg ataattatac ctaccctgct
     4681 gcaaacttcc tgcgaaagct cgtgaaagcc aagccgtgaa cggataatct cattatgact
     4741 tgataacaga atctgtaatt taccagcttc ttttattcgg tctctaatta gtattccaat
     4801 aattttagtc tttttcatag cttttattta aaaataaagg tctctctctc cctgttttat
     4861 tctttcaatt cgttttctta cttcgattac ttgtttttca ttatgtagct cggccatatt
     4921 atcttcaatt actttccatc ctttttcagc tactttcgga tttgcataat ccataatata
     4981 ttcagctaat gttaggattg cgttaggggt acaaaaacgc ttaataaagc ccggaaccga
     5041 aaattccata aaatgctcgc cagttcttcc atgccggtaa caagcagtac agaatgaagg
     5101 taggtaattt ttatcaagca gctcctcgat aacgtcactc agcgatctgg catcatttac
     5161 tctaaactgc tctctattaa ggttttgctc ttcattaaga gtgcttgaat agttgcctat
     5221 ctctaatttt gttccgccat ctatctgcga tactccatat tgcatcacct ctgaacggac
     5281 ttccggggct tcgcgggctg tgagtatcat acctgtgtat ggcactgcca gtcggagtat
     5341 cgctactaat tttgcaaaat ccttatcatt taccatatat tttaggtcaa tgttgaactc
     5401 tgaggcatct tttattcttg ggaatgaaat agtatgcggt ccgacattat atacagcttc
     5461 aaggtgattg gtatgacgca caagccccat tacctcgaat cgccaatcgt aaaggccgaa
     5521 caatgctcca atacctacat catctaatcc ggcttcctgt gcccgatcaa gcgatgtaag
     5581 acgataattg aaatcgcgct ttggcccgct tggatggtaa aatttataca tttcgggatg
     5641 ataagtctcc tgaaagatct gataagttcc aattcctgca ttcttcactg ttctaaaacc
     5701 ttctatgtca agaggagcag catttatgtt aacccgtctt atctctccgt ttcctttctt
     5761 tacgccataa accaatttta cggtgtgggc aatatattct gggctataca tcggatgctc
     5821 tccataaaca aggatcaacc gtttctggcc attctcttca agagcttcgg tttcgcgaat
     5881 gatctcctca tcagtcaatt ttcgcctaat ggcaaacctg ttggaagctt taaagccgca
     5941 atatttacaa ttgttaacac aatgatttcc aatatacaat ggtgcaaaaa gtacaattcg
     6001 atttccatat acttttttct ttaatgtacg tgctccctct ttaatttctt caactaattc
     6061 aggattgtcg gcattgatca atgcagcagt ctcttcgagg gtgagacgtt gcttctcaag
     6121 tgattttgcg ataatctcct ttacacgctc tttggtaggt tttgtattat taagaaatcc
     6181 ccataattcg gccggatcaa taaatggctc catccgacga tcgggtatgg tacatttttc
     6241 aggtgaaaac ttcataattg ttttattatc ttggtaaaaa taataggtaa aaaaataatt
     6301 tattttttaa aaatttctaa ttctaaatat cccgacattt aaacaggcag tacaacctta
     6361 aacgttgatc cttcattgag tttgctctct acagaaattt cgccattgtt aaggtcaaca
     6421 atttttttaa ctatcgaaag tcccaatcca cttcctgata tatttctggt ttggtcgctc
     6481 tttattcgca taaaatcttt gaaaagctct tgcaaatttt cttcgttaat gccaatgcca
     6541 ctatctttaa ccttaat
//
LOCUS       DAOOAI010000107         6549 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_13857_length_6549_cov_4.869726, whole genome shotgun sequence.
ACCESSION   DAOOAI010000107 DAOOAI010000000
VERSION     DAOOAI010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6549)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6549
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13857_length_6549_cov_4.869726"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1143)
                     /locus_tag="PK345_07565"
     CDS             complement(<1..1143)
                     /locus_tag="PK345_07565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005794923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA binding domain-containing protein"
                     /protein_id="HPX34910.1"
                     /translation="MTLKEIIKLPEGRRLEFKGALPEKAELANTIIAFANDAGGELYI
                     GVRNNPREIVGLPEDELIKIEEKISNIIFDRCYPAILPEITFLTEEDKHIIRVTVYRG
                     STPPYYSKEKGKLKGTYIRVGSSNRLADKEIISELERRKQNISFDSELVMDKPVSELS
                     IETFKQMYHDKTDELLDTQTLRKLELIKTVSGTEYPTNALILFSDDMLRNSVFPFAKV
                     ECARFKGVLSEEFIDQKSITSNIALQAEEAYNFVLRHINKGAVVEGVYTVSRWEYPVK
                     AIREAIRNAIVHRDYSLTGKDVKVAVYDDMVEITSPGLLPPSIDYSAMESRQSDARNK
                     IIAPVFKRMGIIDQWGNGLKLISDELKEYPQIEFRWKEVGLSFQVQF"
     gene            complement(1301..1726)
                     /locus_tag="PK345_07570"
     CDS             complement(1301..1726)
                     /locus_tag="PK345_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34911.1"
                     /translation="MYNSVNQTPLVLEYCQAMLDLQGTIPIYIQHAWRGAQDCLYSVM
                     LSNPPERSYIICIRALEAALALNEPLFGGNEIELIKDYINSGLSWHPKSSVLLELKQK
                     YNQQSGTTLSKENLLARQAIGEVLLPLIRGLVNKNNGIY"
     gene            complement(2252..2413)
                     /locus_tag="PK345_07575"
     CDS             complement(2252..2413)
                     /locus_tag="PK345_07575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34912.1"
                     /translation="MNEVYYCGNCRRQQRSSEGERCKVCGKTTVSWYTNRETERDALR
                     KWKQWNGVK"
     gene            complement(2512..3267)
                     /gene="nadE"
                     /locus_tag="PK345_07580"
     CDS             complement(2512..3267)
                     /gene="nadE"
                     /locus_tag="PK345_07580"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HPX34913.1"
                     /translation="MKPFNAKQVKEQAVQWIRNWFEENGKGCKAVIGISGGKDSNIVA
                     ALCVETLGKERVIGVTMPNGEQKDISDSMRLINHLGISYFIINIAGACNALISAVKEQ
                     LGTSSKEVSEQTLINLPARVRMTTLYAISQSMNGRVANTSNLSEDWVGISTRYGDGAG
                     DFAPLGGLTMHEVIAVGKELGLPIDLVEKVPVDGLCGKTDEDNFGFTYAVLDRYIRTG
                     QCDDLKTKEIIDKKHRNNLFKMQPIPNFEVNTK"
     gene            complement(3482..3566)
                     /locus_tag="PK345_07585"
     tRNA            complement(3482..3566)
                     /locus_tag="PK345_07585"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3530..3532),aa:Ser,seq:tga)
     gene            3737..5941
                     /locus_tag="PK345_07590"
     CDS             3737..5941
                     /locus_tag="PK345_07590"
                     /inference="COORDINATES: protein motif:HMM:NF019335.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HPX34914.1"
                     /translation="MVQGFFSVSVMCLLFLFAAISDLQASPFLKGNHNLPDSATAIHL
                     DKKTDISEGFGSRIIDSGAPVSKTDSEEVRCLPANDLSNTLVYLPGISPSATDGSGLQ
                     SQLSIRGFRNLLGSESVILMVDGIPINDQEIGFVSWNFLPLSIISSIEVLRGGSSALY
                     GDPAVGGAINLRTEINKKRVIAVKTGIGSFKNQTKSVVYGANSEKVNYSLYINDRRGD
                     GYRENSNWRSTTFGGRVNINLRNNSSLGLSIYNQLLNFGIAGPASERMIKEDPKASAP
                     CYHHDEIENNRHLFKLDFKQKVDKKTNLEAVLAYSLKKLKETKSFAEPPLALDPFNLK
                     PIDVYDTTLYANTLKRSVSDDHGGISIRIFHNNPQVGFKLIGGVEADLNIFDNSVNKL
                     FKGFEYDYQKGYQEIDEPEFAGNGFRFRSAAFISSQIRIFDNLNFFAGLRYDNISEDF
                     SSDIPVADTSMTRTYNALSPRLSLNLGSGETFNYKGNIIISYSNAFKLPTIYQRTDLT
                     RLSHAVFYPAGSGTYQMEVIQGAPFSNGELKQQYNKNYELGINQFYRFSEKFAGEISL
                     TGFVIDVKNEIDFDYKNLKYVNHQETRHAGLEVMISLLYKNSWKGFININSVKATVGT
                     GMFKGKYLPNAPGLSQSIGVVYSPDHGVGGSLVHHGASGIYIDIENSSKLDAYGVLST
                     RINYRYKWLTLNFDVNNILNNSYSTNGYLLNETRFFYPAAGRSLYFGLQITI"
     gene            complement(6009..>6549)
                     /locus_tag="PK345_07595"
     CDS             complement(6009..>6549)
                     /locus_tag="PK345_07595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34915.1"
                     /translation="HFKFSINKPQNGGFSPVYHIHDPYTPLLKSYKLAIKPTGFDPAL
                     RNKLLIVKLDTKNEKPIPAGGKWDGKFLTTNVREFGSYTVLADTKPPTIKPANIQNGK
                     NISGQGSIRITVTDDLAGIETFRATMNGKWILMDWDPKRNLLEYKIDEHTKKGKNTFR
                     LSVVDTRGNEAVYEATLYR"
BASE COUNT         1912 a         1257 c         1230 g         2150 t
ORIGIN      
        1 aaactgtacc tgaaatgaaa gtcctacctc tttccaacga aattcaattt gagggtactc
       61 tttcaattcg tcggagataa gtttcagccc gtttccccat tggtctataa ttcccatacg
      121 cttgaaaaca ggtgcgataa ttttattacg agcatcgctc tggcgacttt ccattgccga
      181 gtaatcaata gaaggtggta aaagtcccgg gcttgtaatt tcgaccatat catcatatac
      241 agccacttta acatcctttc ctgtaagcga ataatcgcga tgcacaatag cattccttat
      301 tgcttcacga atggctttta ccggatattc ccatcgcgaa acagtataaa ctccctctac
      361 aacagcacct ttattgatgt gtcgtaacac aaaattatag gcttcttctg cttgtaaggc
      421 aatgtttgac gtgatactct tttggtcgat aaactcttcg gataacacgc ctttgaatct
      481 ggcacattcc acttttgcaa aagggaaaac agagttacgg agcatatcat cagaaaacaa
      541 aatcaaagca ttggtagggt attctgtccc gcttactgtt ttaataagtt ctaatttccg
      601 taatgtctga gtatccagta attcgtctgt tttgtcatga tacatttgct tgaaagtttc
      661 tatacttagt tcactgacag gcttatccat taccaattcg ctatcgaaag agatgttttg
      721 ttttctacgc tccaattcgg aaatgatttc cttgtcggct aaacggtttg atgagccaac
      781 acggatatac gtccctttca actttccttt ctctttcgaa tagtatggtg gtgtacttcc
      841 tcgataaaca gttactctga taatatgctt atcttcttct gtgagaaacg taatttcagg
      901 taaaatagcg ggatagcaac ggtcgaaaat gatattactt attttctcct caatttttat
      961 taactcatct tcgggtagcc ctacaatctc tctcggattg tttcgaacac caatatataa
     1021 ttctccaccc gcatcattgg caaaagcaat aatggtatta gccaattctg ccttttcagg
     1081 tagtgcaccc ttaaactcca agcgtctgcc ttcgggtagt tttataatct ctttgagggt
     1141 catatctact ttaatctttc tttgtctatc tataagtttt tacctgacag taatataaaa
     1201 ttatatgaat ttacagcaca caaaagtaca ttttttgtat caattccaag cgttactttt
     1261 gattttatta ctcccctaat ctatttcaca attcagcttt tcagtatatg ccgttatttt
     1321 tgttaaccaa tccacgaatc aacggcaaca gcacttcccc aatagcctga cgggcaagga
     1381 ggttttcttt ggagagcgta gttcccgatt gttgattgta tttctgcttt aattcaagca
     1441 agacggatga ttttggatgc caacttaaac cggagttgat ataatctttt atcaactcta
     1501 tttcattgcc gccaaaaaga ggctcattca aagctaaagc tgcctcgagc gctctgatac
     1561 agataatgta ggatctttcc ggtggattag aaagcatgac actataaaga caatcttgtg
     1621 cacctctcca tgcatgctga atataaatcg ggatggtgcc ttgtaaatcc agcatagcct
     1681 gacaatactc caacaccaaa ggcgtttgat ttacgctgtt gtacataaac agtttttttg
     1741 gcttgctaaa taggtttttt cacaagcttt gaaaacacct acacgatgtt gtgcttgaag
     1801 aagcaaaaga aaagctataa gatcattcgg gaagttttcc aaatttttct ctgccttctt
     1861 taatgcaatt tcatactctc ctttttctaa atgggaggta atttcctccc atttatcgcg
     1921 attaaacaaa attcatatgc tcattggacg catttctgcc tctgtaaaca atggccaacg
     1981 acgattttct tcagttatta cagctggttc agcctccttt tttgttcact ttctttaatg
     2041 ggcgcttcca aaacaatatt ctgccgggat tcgctttttt ctcatcactt ctttatttgc
     2101 ctttttaggt gcaataaata ataagtaaat cacacttacg acaaatgata aaatgataat
     2161 attgataata cttattattc aataccctaa tccgtgtcgg gcacaagtgt tactttacta
     2221 ttcgactttt cgggatacgc cttagtaatt tttactttac accattccat tgtttccatt
     2281 tacgtagagc atctcgttct gtttctctgt tggtgtacca actaacagtt gtttttccac
     2341 aaactttaca ccgctcacct tctgaggagc gctgttgacg acggcagttg ccgcaataat
     2401 atacttcgtt catatctgat ttatttttgc aaagttaatt atttgtattc gttattttac
     2461 taaaaatatt tttttaattt tcgaattctg tattgattac aacagttacg attattttgt
     2521 attaacctca aaatttggaa taggttgcat cttaaacaaa ttgttacgat gctttttatc
     2581 aataatctct ttagtcttga ggtcatcgca ctgtccggta cgaatgtagc gatcgagtac
     2641 agcataagta aagccgaaat tgtcttcgtc agttttaccg cagagaccgt caacaggaac
     2701 tttttccact aaatcgatgg ggagtcccaa ttctttacct acggcgatta cctcgtgcat
     2761 tgtaagaccg cctaagggtg cgaaatcgcc agcgccgtca ccgtaacggg tcgagatgcc
     2821 tacccaatct tcggaaaggt tactggtgtt tgccacacga ccattcatgg attgcgagat
     2881 ggcatataat gttgtcatgc gaacacgtgc cggtagattg ataagcgtct gttcactaac
     2941 ttctttactt gaagtgccga gttgctcttt tactgcagat attagtgcgt tgcaggcgcc
     3001 agcaatatta ataataaagt agcttatacc aagatgatta ataagtctca tgctatcgga
     3061 gatgtctttc tgttcgccat tgggcatggt tacaccgata acacgttctt tgcctaatgt
     3121 ttctacacag agtgcagcca caatattgct atccttacct cctgatatgc cgattactgc
     3181 tttacaaccc tttccatttt cttcaaacca attcctaatc cattgaacgg cttgctcttt
     3241 tacttgtttt gcattgaatg gtttcataag agtttattat ttttcctact ctatatttct
     3301 tatttggctt ttttggttat tgcagttgtt tgttgacatg gaaaattatg atgaggcgtg
     3361 tgggtggatt aggattcctg aagataggag ggtagggaag agaaaactta aaaccggtaa
     3421 aatgcaaatg taaaaagcaa cgaggtaaag atcgtaatta atgaatacct cgattagtta
     3481 gcggagagtg agggattcga acccccggac cctttcaggt caacggtttt caagaccgcc
     3541 gcgatcgacc gctctgccaa ctctccggcg caaaagtaca cttttttgaa aatttcaaaa
     3601 ctttttgttt caatttagct aaatagattg agattatgtg gaaaagatga tctgttaatc
     3661 tggttattaa cgaatatagt gggtcagaat aataatgata attttgctaa accgatattt
     3721 acaatctgat tgatttatgg ttcaaggttt tttttctgtc tcagtaatgt gcttactttt
     3781 tctttttgct gcaatctctg atttgcaggc ttctcctttc ctaaaaggta atcacaactt
     3841 accggattct gctaccgcaa ttcatcttga taaaaaaact gatatttctg aaggttttgg
     3901 aagtcgaatt atagattcag gcgctccggt aagtaaaaca gattcggaag aggtacgatg
     3961 cttgccggca aatgaccttt ccaacacatt ggtttattta cctggaattt ctccctctgc
     4021 caccgacggt tctggtttgc aatctcaact tagcattaga ggatttcgta atttgcttgg
     4081 atcggagtca gtaatattaa tggttgatgg tatcccgatc aatgatcagg agattgggtt
     4141 tgtaagctgg aattttcttc ctttatcaat catcagctca atagaagttt taagaggtgg
     4201 ctcatcggcg ctttatggcg accctgccgt tggaggcgct ataaatctac gtactgagat
     4261 taacaaaaag agagtgattg ctgttaagac aggaattggt tcatttaaaa atcagactaa
     4321 aagcgtagtt tatggtgcaa actctgaaaa ggtaaactac agcctttata taaatgacag
     4381 aaggggtgat ggttatagag aaaatagcaa ttggagatca acgacttttg gaggaagagt
     4441 aaatattaac ctcagaaaca attcatcgct tggtcttagc atttataatc agttactcaa
     4501 ttttggaatt gcaggtccgg ccagcgaaag aatgataaaa gaagacccta aagcctcagc
     4561 gccttgttat catcatgatg aaattgaaaa caaccggcat ctttttaaac ttgactttaa
     4621 acaaaaagta gataaaaaaa ccaatttaga agctgtatta gcatatagtt taaaaaaatt
     4681 gaaagagaca aaaagctttg cagaaccacc attagcgctc gacccattta atttaaaacc
     4741 tattgacgta tatgacacca ccctctatgc caatacatta aaaagaagcg tttctgacga
     4801 tcatggcggg atttctataa ggatttttca taataaccca caagttggtt ttaagctgat
     4861 aggaggagtt gaagctgatc ttaacatttt tgacaattct gtaaataagc tttttaaagg
     4921 ttttgaatac gattatcaaa aaggatatca ggagatagac gaaccggagt ttgcaggtaa
     4981 cggattccga tttcgatcgg cagcttttat aagtagtcaa ataaggattt ttgataatct
     5041 gaactttttt gccggtttga ggtatgataa tatttcggaa gatttcagca gcgacattcc
     5101 ggttgcagat acttcgatga caagaactta caatgcttta agtccaaggt tgtcattaaa
     5161 tttgggttcc ggagagacgt ttaactacaa agggaatatt atcattagct atagtaacgc
     5221 ttttaagttg cctactatat atcaacgaac agatctgaca aggttgagcc atgccgtttt
     5281 ttatcccgcc gggtcaggta cttatcaaat ggaggttata caaggcgctc ctttttcgaa
     5341 tggagagcta aaacaacaat ataacaagaa ttatgagttg ggaataaatc agttttacag
     5401 attttcagag aaatttgccg gtgagatcag cctaacagga tttgttattg atgtgaagaa
     5461 tgaaattgat ttcgattata aaaatctcaa atacgtcaat caccaagaaa cacgtcatgc
     5521 agggcttgag gttatgataa gtttgctata taaaaatagt tggaaaggat ttattaatat
     5581 aaattccgta aaggcgacag tgggcacagg gatgtttaaa gggaaatact taccaaatgc
     5641 gccgggatta tcccagtcaa taggggttgt ctattctcca gatcacgggg ttggcggctc
     5701 tttagtacat catggggcat cgggaattta tattgatatc gaaaacagtt cgaagttgga
     5761 tgcctatggg gttttaagca cacgaatcaa ttatcggtac aaatggctga ccctgaattt
     5821 tgatgttaat aatattctaa ataattcgta tagcacaaat gggtacttgc ttaatgaaac
     5881 ccgatttttc tatcccgcag cgggtaggag cttatatttt ggattacaaa ttactatcta
     5941 accaaaaata gctttcatcc aaagaaggcc taaatcttta tcaagggtta ttattttcaa
     6001 agtatcaatt atctgtaaag ggttgcctca taaacagctt catttcctcg tgtgtcaacc
     6061 actgaaagcc ggaaggtgtt tttacctttt ttggtatgtt catcaatttt atattctaac
     6121 agattccttt taggatccca gtccatcaaa atccatttgc cgttcatagt tgccctaaag
     6181 gtttcaatcc cagccagatc gtctgttaca gtgattctta ttgaaccttg ccccgagata
     6241 tttttcccgt tttggatatt tgcaggcttt atagtaggag gtttagtgtc ggctaacact
     6301 gtgtaactac caaattctct aacgtttgtc gtcaaaaatt tgccatccca tttaccgcct
     6361 gcaggaatcg gtttctcatt tttagtatct aactttacaa tcagcagctt atttcttaat
     6421 gctggatcaa agccagtcgg tttgatagca agcttataac tctttaagag cggagtataa
     6481 ggatcatgga tatgataaac cggagaaaaa ccaccgtttt gaggtttatt aattgaaaac
     6541 ttaaagtga
//
LOCUS       DAOOAI010000108         6457 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_14095_length_6457_cov_5.615120, whole genome shotgun sequence.
ACCESSION   DAOOAI010000108 DAOOAI010000000
VERSION     DAOOAI010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6457
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14095_length_6457_cov_5.615120"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            225..569
                     /locus_tag="PK345_07600"
     CDS             225..569
                     /locus_tag="PK345_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004328453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HPX34916.1"
                     /translation="MPKNKKLKGDLVYSTNPDLMLRLSQEKPIITPPPHQQDLKVWID
                     RKQRKGKVATLITGFVGSDDNLTALARLLKTKCGVGGSAKDGEILIQGDIREKVMEIL
                     TQEGYKVKKAGG"
     gene            723..1277
                     /locus_tag="PK345_07605"
     CDS             723..1277
                     /locus_tag="PK345_07605"
                     /inference="COORDINATES: protein motif:HMM:NF028012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5063 domain-containing protein"
                     /protein_id="HPX34917.1"
                     /translation="MEQEEILQEPVYSQQVLEFLAVAHKYCQFMEQVYRQSAENISDF
                     LVQILPLLYFRGSLLPVINHIEIEVVEKYVTEEEWQALFNDLRQKFLDNDEYWFVEND
                     NPHNDLIRGSLSDNLADIYQDLKDFVILYQRPFRNVKEAAVWDIRQLFRSHWGFRVVN
                     VLKVLHYQLYGSENYRAINEEEGY"
     gene            complement(1431..1517)
                     /locus_tag="PK345_07610"
     tRNA            complement(1431..1517)
                     /locus_tag="PK345_07610"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1481..1483),aa:Leu,seq:gag)
     gene            1624..3177
                     /locus_tag="PK345_07615"
     CDS             1624..3177
                     /locus_tag="PK345_07615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018666580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4301 family protein"
                     /protein_id="HPX34918.1"
                     /translation="MLTEKDHQQIKEHGISIEQVEQQIAAFKAGFPFVNLISPAKRGD
                     GIHAFDEKRLAELRELYKDNSEQFEIIKFVPASGAASRMFKHLFEFLDWYDGSNEAIE
                     RLEKDKSFNSVFYFLKNINRFAFIKDLKDALSKDGLDLSTLLEEKRYDVPIRYLLFDK
                     GLGYAELPKALLMFHQYDGFSRMAIGEHLVEGAHYARCRDGVVNIHFTISPEHKTKFD
                     QFIECRKKEYESSYGVKYNIGFSEQKPSTDIIAVDMNNEPFREDDGKLHFRPGGHGAL
                     IENLNDLKGDIIFIKNIDNIVPDRLKEPTTLYKQVIGGLLIELQEKTFEYLRLLEDGK
                     VDADKLNEIATFAERDLMIQFPDRFNRMSLSQKTYYLFERLNRPMRVCGMVKNEGEPG
                     GGLFWVKNSKDEVSLQIVEFSQINLQDAQQKQLAMEATHFNPVDLVCAVRDFKGNQFE
                     LTNYIDHTTGFISVKSKDGRDLKAQELPGLWNGAMADWITVAVDVPIITFNPVKTIND
                     LLREQHQYN"
     gene            3225..4247
                     /locus_tag="PK345_07620"
     CDS             3225..4247
                     /locus_tag="PK345_07620"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HPX34919.1"
                     /translation="MNFRLFLSLLLVATLAIMSNGVSAQDTFSIVAVDTITGEVGGAG
                     ASCLDDSAIAGGVLIISDIIPSRGSIHTQSYWHPTNQQNAHNRMMEGMSPDEIIEWLV
                     ANDIQNTPQKRQYGIVDFDQEGHPRSAAFTGTSCHNYKNHIIGPNYAIQGNILLGQQI
                     LDSMEYRFLNTEGSLAEKLMSALQGAKVPGADTRCLNEGTSSLSAFVRVAKPNDHPDS
                     LWCHLVVTSTPYGVEPIDSLQVLFDQWLIWTNINERKLNEASISNVFPNPASQTFTID
                     IKPVYELNQATIRIFNNRGLLIDEAQWQGEPIIFNTVKYTTGIYYFQVSGNNMILDSG
                     KFNIQK"
     gene            4260..4382
                     /locus_tag="PK345_07625"
     CDS             4260..4382
                     /locus_tag="PK345_07625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34920.1"
                     /translation="MAKDRFVGLAFDAVNSGNTQRLTDFTTDNAVFRLTPKLQE"
     gene            4644..5522
                     /locus_tag="PK345_07630"
     CDS             4644..5522
                     /locus_tag="PK345_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024762.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like domain-containing protein"
                     /protein_id="HPX34921.1"
                     /translation="MDEKQINSELNALINLLDEPDEFVFDKVKERFFSYGLEAVPLLE
                     DVWDSSYEHTQVQKRIEGIIHEIQFNNVIKLLSQWKEKAEPDLLAGAMIIAKYHYPEM
                     KDKVVIDKIGRIIQDVWLELNDNLTFLEKIKILNHIIFDIHGFKPNKSDLFAPSTSYI
                     NLMVESKMGNYESLGILYLIVAQSHNIPLYGVDLPRQLIMACTDKLIRPGDPADKHDV
                     LFYLTAHHTGIVFTRREVEKFIEQMNLETKPSYFLPCDNITVIRRLINYLILSYEKRG
                     MMDKIEDLKKLKQVLE"
     gene            complement(5566..6114)
                     /locus_tag="PK345_07635"
     CDS             complement(5566..6114)
                     /locus_tag="PK345_07635"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HPX34922.1"
                     /translation="MYWLDIIIGVPLLLYAIVGFAKGFVRSFASFAALILGVLLGMKF
                     SNWLSTVLAQHLDFSKSIIFIISFIIIFITVIIVINIIGKILDKFIEKTVINLPNKIL
                     GLIFGLLKGVLILSVIIALYNWFDPNGKFLKLEKRDNSLLYNPISKVVPKIMTDVFNY
                     QLDNQDGKMPKKEESAPLNKTV"
     gene            complement(6114..>6457)
                     /locus_tag="PK345_07640"
     CDS             complement(6114..>6457)
                     /locus_tag="PK345_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HPX34923.1"
                     /translation="SELSGGMIKRVAIARAIALNPRYLFCDEPNSGLDPQTANLIDNL
                     ISEITEEFNITTVINTHDMNSVVEIGENIIFIHDGKIWWEGDNNEILNSENKELNDFV
                     FASKLTRKLKT"
BASE COUNT         2102 a         1159 c         1283 g         1913 t
ORIGIN      
        1 gccattaaaa ctcagcctct ctacagaaat attaataata agggtttcag attcattgac
       61 cttcaatact ttcatcgcta aaataactca aaaccttgca atatggaaca cctaaagtaa
      121 atattgttat taacagtata gtgttgataa acaatactta atcatgtttt taaggatcgt
      181 ctaattagag ttttaacttt acctttgcaa aaaacaagta tggaatgccg aagaacaaga
      241 agttaaaagg ggatttggtt tactccacta atccggacct tatgttaaga ttatctcaag
      301 agaagccgat aatcacacct ccaccgcatc agcaagatct gaaagtatgg atagatagaa
      361 aacaacgtaa aggaaaagtg gctacactca ttaccggatt tgttggaagt gatgataacc
      421 tgaccgcttt agcaagatta ttaaaaacca aatgcggtgt tggaggatcg gcaaaagatg
      481 gtgaaatact aattcagggc gatataagag aaaaagtgat ggaaattctt actcaagagg
      541 gttataaggt aaaaaaggca ggagggtaat ttggtttttc aaagaatgag aatcccgaat
      601 tactaaatag aaaagcgtta aataggcttt tttctgaata agtggtttgt ttttacttaa
      661 gagagagcct gaaagaattt ttcaaactct atatatcttt gatctttaat aattaaacaa
      721 cgatggagca ggaagagatt ttacaagaac cggtttattc gcagcaggtg ctcgaatttt
      781 tggctgtagc acataagtac tgtcaattca tggagcaagt gtataggcag agcgccgaaa
      841 acattagtga tttcctggtt cagatattgc cattactcta tttcagaggc tcattgctgc
      901 ctgttattaa tcatattgag attgaggtgg ttgaaaaata tgttaccgaa gaggagtggc
      961 aggctttgtt taatgatttg cggcaaaaat ttttggataa tgacgaatac tggtttgtag
     1021 aaaatgacaa tccgcataac gaccttatcc gaggtagtct atcggataac ctggcggata
     1081 tttatcagga tctaaaagat tttgtaatcc tttatcagag gccttttcgt aatgttaagg
     1141 aggcagccgt ttgggatatc cgacaattat tcaggagtca ttggggattt agggtagtta
     1201 atgtgttaaa agtacttcat tatcagcttt atggaagtga gaattatagg gcgataaacg
     1261 aagaagaggg ctattaggtg ttgctgcagg ctcataaaaa atgcggtgcc tcacaagtac
     1321 ccaactgcta tgcttcgact tcgcccccaa tctcagcgcc ttgcatttgg acacttctaa
     1381 gacaccttgc tgttttttag ttaaatattt gttttaaaac agccccacct tggtgcccag
     1441 aacaagattc gaacttgcac acccgtgaag gcgccagccc ctcaagctgg tgtgtctacc
     1501 aattccacca tctgggcaac tatctgacga caaaagtaaa aataaatttt taaatgattg
     1561 gtttttggca aaaaaaactt ttagatttgc aagaaataaa gaaaacttta aataacatga
     1621 tatatgttaa ctgaaaaaga tcatcaacag ataaaagaac atggtatttc cattgaacag
     1681 gtagaacaac agatagctgc tttcaaagca ggattcccat ttgtcaacct gatttctccg
     1741 gctaaaagag gcgatggcat acacgcattt gacgaaaaga ggttggcaga attaagagag
     1801 ctttataaag ataacagcga acagtttgag ataataaagt ttgtaccggc atcgggggct
     1861 gcaagtcgaa tgtttaaaca cctgtttgag ttcctcgatt ggtacgatgg tagcaacgag
     1921 gcaatagaga gacttgaaaa ggacaaaagc ttcaactcgg ttttttattt tttgaaaaac
     1981 ataaaccgtt ttgcttttat taaagatttg aaagatgctc tttcgaaaga tggtttagat
     2041 ctttccacct tgttggaaga gaagagatat gatgtgccaa ttagatattt gctttttgat
     2101 aaaggcttgg gatatgccga actgcccaaa gcattgctta tgtttcatca atacgatggc
     2161 ttttcgagga tggctattgg cgagcattta gttgaaggcg ctcattatgc acgttgtcgg
     2221 gatggcgtgg ttaatatcca ttttacaata tctcccgaac ataaaaccaa atttgatcag
     2281 ttcatcgaat gccgaaaaaa ggaatatgaa tcctcttacg gagtaaagta caatatcgga
     2341 ttttctgagc aaaaaccctc cactgacatc attgctgttg atatgaacaa tgaacctttt
     2401 agggaagatg acggaaaact ccatttcaga cctggcgggc atggagcgtt aattgaaaat
     2461 ctgaatgatc tgaagggtga tatcattttt ataaaaaata ttgacaacat cgtacccgac
     2521 cggcttaaag agccgacaac tctctacaaa caagtcatcg gcggcctatt gattgaactg
     2581 caggaaaaaa ctttcgagta tctacgacta ttagaagacg gaaaagtaga tgccgacaaa
     2641 ttaaacgaga tagccacttt tgctgaaaga gatctgatga tccagttccc ggatagattt
     2701 aatcgaatgt cgttatctca aaaaacctat tatctctttg agcgccttaa ccggccaatg
     2761 cgtgtttgcg gcatggtaaa aaacgaaggc gaaccgggcg gaggactgtt ttgggtaaaa
     2821 aatagcaaag atgaagtatc gttgcagatt gttgaatttt cacaaatcaa tttacaggat
     2881 gcgcagcaaa aacaacttgc tatggaagct actcacttta accctgttga tctggtttgt
     2941 gcagtaaggg attttaaagg caatcaattt gaactaacca attacataga ccatactaca
     3001 ggctttatat ctgtgaaatc aaaagatggc agagacctta aagcacagga gctacccggc
     3061 ctatggaacg gcgctatggc cgactggatc accgttgccg ttgatgtgcc cataattact
     3121 tttaaccccg taaaaacaat aaacgattta ttgagagagc aacatcagta caactgataa
     3181 ctacataaag gaatgtttaa ctatgaaata aaaatttaaa gactatgaat tttcgactct
     3241 ttttaagctt attgttggtt gctacattag caatcatgtc aaatggcgta tcggcgcaag
     3301 atactttctc tattgttgct gttgatacta taaccggaga ggtaggcggc gcaggcgcct
     3361 catgccttga cgattctgca atagcaggtg gcgtgctgat aattagtgat attattccga
     3421 gccgaggatc aattcacaca caatcttact ggcatcctac taatcaacaa aatgcccaca
     3481 acagaatgat ggagggaatg tcgcccgatg aaatcatcga atggcttgtt gccaatgata
     3541 ttcaaaatac cccccagaaa agacaatatg gaatagtgga ttttgatcag gaagggcatc
     3601 cccgtagcgc tgctttcaca gggacaagct gccataatta caaaaatcac atcatagggc
     3661 caaactatgc aatacaaggt aacatacttt taggtcaaca gatactcgac agtatggagt
     3721 ataggttttt aaatactgag ggttctttag ctgagaagct gatgtcggca ttgcagggcg
     3781 ctaaagtacc gggagcagat actcgttgcc tcaacgaagg cacttcatcg ctatctgcat
     3841 ttgttcgggt ggcaaaacca aacgatcacc ccgacagcct atggtgtcat ttagtagtaa
     3901 cttccacacc ttatggtgtt gaacctattg actcgctgca agtacttttc gaccaatggc
     3961 tcatctggac aaatataaac gaacgcaaac ttaatgaggc atcaatatca aatgtgtttc
     4021 caaacccagc cagccaaacg ttcacgattg atatcaaacc cgtttatgag ttgaatcaag
     4081 ctacaatcag aattttcaac aatagaggcc tattgattga tgaagctcaa tggcaaggtg
     4141 aaccaatcat ttttaatact gttaaataca caaccggaat ttattatttt caggtttcag
     4201 gaaacaacat gatcttagat tccggaaagt ttaacataca aaagtaatta actgatatta
     4261 tggctaaaga tagatttgtc ggcttggcat ttgacgctgt taatagtggc aatacgcaaa
     4321 gacttactga ttttacgacc gataatgctg tttttagatt aactccgaag ttgcaggaat
     4381 agggaaaatt acagattatc tgatttttgt taataattca gaactttata gcagataaat
     4441 ttgttttcga tcagtacacc ataatagcta aaatcctcta tcttatcata cgtgtttttt
     4501 taagtcatca aattggtcat tttcattaat attgtaccgt tcaaatagat tttggcatga
     4561 cttctattag atacttaaga tattttacac cgatttacca taacactttt gcttattgat
     4621 tacattgaaa attcgctgct aaaatggatg aaaaacaaat aaatagtgag ttgaatgctt
     4681 taattaattt gttggacgag cctgacgaat ttgtattcga taaagttaaa gagcgttttt
     4741 tcagttatgg ccttgaagcc gttcctctgc ttgaagatgt ctgggactcg tcatatgagc
     4801 atactcaggt gcaaaaacga attgagggta tcatacatga gatacagttt aacaatgtca
     4861 ttaaattgct tagccaatgg aaagaaaagg ctgaacccga cctgctcgcc ggagctatga
     4921 ttatcgctaa atatcattac cctgagatga aagataaggt ggtgattgat aaaattggca
     4981 ggattataca ggatgtatgg ttggagctga atgataacct cactttttta gaaaagataa
     5041 aaatcctgaa tcatatcata ttcgacatcc acggctttaa gccaaataag tcggatcttt
     5101 ttgcaccctc cacctcctat atcaatctga tggttgaatc gaaaatgggt aattacgaat
     5161 cactcggtat tttatatttg atagttgccc aaagccataa tattccgctt tatggtgttg
     5221 atcttcctcg tcagcttatt atggcttgta ctgataagct tatcaggccg ggtgatcctg
     5281 ctgacaaaca cgatgtcttg ttttatctaa ccgctcatca tacaggtatt gtattcacac
     5341 gtagagaagt agagaagttt attgagcaaa tgaatttaga gaccaaaccg agctattttt
     5401 taccttgtga taatattaca gttatccgaa ggctaatcaa ctaccttatt ctatcttatg
     5461 aaaaaagagg catgatggac aagattgaag atttgaaaaa attaaaacag gtattagaat
     5521 agtagtgtgc ttacttcaaa acttttcatt tttacaaatc gtccctcata ctgttttgtt
     5581 taaaggggca ctttcctctt ttttcggcat tttgccatct tgattatcaa gctggtagtt
     5641 aaaaacatca gtcattattt taggcacaac cttgcttatc gggttatata ataatgagtt
     5701 atcacgtttc tccaatttca aaaatttgcc attaggatca aaccagttgt aaagcgctat
     5761 gattacgcta aggataagta cacctttcag caagccaaat atcaatccca atattttgtt
     5821 gggcaaatta attacagtct tctcaataaa tttgtcaagt attttcccta taatattgat
     5881 aacgatgata actgtaataa aaatgattat aaaggagatg atgaatatta tactttttga
     5941 gaaatccaga tgctgagcca aaacagtgga taaccaattt gagaatttca ttccaagaag
     6001 gacacccaat attagtgcgg caaatgaagc aaacgatcgc acgaaacctt ttgcgaatcc
     6061 gacaatagca tacaataaca ggggaacgcc tatgataata tcaagccaat acattatgtt
     6121 ttaagcttac gtgtgagctt ggatgcaaaa acaaaatcgt ttaattcttt attttcagag
     6181 ttcagtatct cattgttatc accttcccac catattttac cgtcgtgtat aaaaattata
     6241 ttctcgccaa tctctactac tgagttcata tcgtgcgtat tgatgacagt tgtgatattg
     6301 aattcttcgg ttatctcgct aataagatta tcaatcaggt tagcagtctg tgggtcaagg
     6361 ccagaatttg gttcatcgca gaacaggtat ctcggattaa gcgcaatagc gcgtgcaatg
     6421 gctacacgtt taatcatacc gccgctcagc tccgagg
//
LOCUS       DAOOAI010000109         6328 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_14444_length_6328_cov_5.627292, whole genome shotgun sequence.
ACCESSION   DAOOAI010000109 DAOOAI010000000
VERSION     DAOOAI010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6328)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6328)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6328
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14444_length_6328_cov_5.627292"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..334)
                     /locus_tag="PK345_07645"
     CDS             complement(<1..334)
                     /locus_tag="PK345_07645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34924.1"
                     /translation="MRLRNFNIGRHLRDRLHYSWMLLVLAITMIVPVALLSGLIYKSS
                     FLLSEQSFFQILFSNQWKPLSGKFGFYPFIISSLWVSVVSLLIAVPICLLTAIHLTQY
                     AKRWVLNLM"
     gene            complement(331..1314)
                     /locus_tag="PK345_07650"
     CDS             complement(331..1314)
                     /locus_tag="PK345_07650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013445128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HPX34925.1"
                     /translation="MRTKTTFTLLIITAFYFFYFILNGCTSQPENRHHKKGELKGTIS
                     ISGAFALYPMTVKWAEEFMQLNPGVRIDISAGGAGKGMADVLSEMVDLAMVSREIAPA
                     EIEKGAWYLAVCKDAVLGTINESNPYVKEIKQYGISRETLTKIYIDVTIENWDQIPGI
                     SANNQKINVYTRSDACGAAEMWGLFFGKNQESLQGIGVFGDPGIADAVKNDKFGIGFN
                     NVIYAFDINTRKKFEGLEIIPIDLNNNGVIDPDENFYDNLDDICAAIGNEQYPSPPAR
                     DLYLISKGEPKNPIVIEFLKWILTEGQQYVNDAGYVRLSESKIETEMQKIK"
     gene            1642..3144
                     /locus_tag="PK345_07655"
     CDS             1642..3144
                     /locus_tag="PK345_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019539057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HPX34926.1"
                     /translation="MDVQIELLILILSVLFFFSILAGKASSKYGVPALLLFLSVGMIF
                     GSDGLGIHFENIQVARNLGALALCVILFSGGLDTKIDEIRPVIFQGVILATIGVFLTA
                     LFTGIFVWWILGMTIKSASIGLITSFLLASTMSSTDSASVFSILRSKGLHLKNNLRPM
                     LELESGSNDPMAYLLVITLIELINFETTPNYWMVSLQVVIQLVVGALIGLTIGKLSIY
                     LINRIKIGNDSLYPILVLTICLFIFSLTYFAKGNGYLAVYVAGLTIGNGKFVHKRTSL
                     KFFDGLAWLGQLIMFLTLGLLVNPRELVPIMIPGLIISLAMIFITRPLTVFLCLLPFR
                     KMPIRDKAYVSWVGLRGAVPIIFALFPLTENVPHARLIFNIVFFCTIISLLVQGTSLP
                     AIAKWLGLTEKPPKIGKIESFDVDFSDDIKSIVTEIEINSTTLSKGNKVMELEIPEET
                     LVVMINRDNNYFIPKGNTILMEKDKLLIITVDHNSLRKTLKNLNIHDSQS"
     gene            3426..6257
                     /gene="uvrA"
                     /locus_tag="PK345_07660"
     CDS             3426..6257
                     /gene="uvrA"
                     /locus_tag="PK345_07660"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013444863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HPX34927.1"
                     /translation="MLEVYGARMHNLRNIDVKIPRNSLVVITGLSGSGKSSLAFDTIY
                     AEGQRRYMETFSAYARQFIGNMERPDVDKIDGLSPVISIEQKSVNRNPRSTVGTITEI
                     YDFLRLLYARIGEAYSINTGEKMVRFTEKEIIDLIMREYEGKQINLLAPMVKARKGHY
                     RELFEQITKMGYPKVRVDGEIRDITFGMKVDRYKTHDIEVVIDRIQLEDSMNERITRS
                     VQAAMKIGKGVIMIHDTKSDQVRHFSSLLMCPTTGISYSEPEPNTFSFNSPYGACPHC
                     NGLGEVSQIDLKKVIPDVKRNIRNGGLAPLGEYKNNWIFRQIEAIGYKYGFTLDTPIE
                     DIPEKGMITILYGSDEIIHVKNEYLGVTSNYSLNFEGIINFILNQDYETSSNQIKKWI
                     SGFMNRIPCPECNGARLKKESLHFKILNKNIAELANMDLFLLHGWIENLPSHVSDRTF
                     KIAREITREISTRIRFLLEVGLDYITLNRSARSLSGGESQRIRLATQIGSQLTGVLYI
                     LDEPSIGLHQRDNRRLINALKQLRDIGNSIIVVEHDKEMILSSDYIVDIGPGAGVKGG
                     LIVGQGKPDSMKDCNTITCDYLSGKKRISVPKIRRSGNGKFLRLEGATGNNLKNITLN
                     LPLGKFVCITGVSGSGKSSLINETLYPILSSHFYRSEKKPLPYKKISGIEFVDKVIEI
                     DQSPIGRTPRSNPATYTKVFDEIRKLYGSLPESKIRGYQPGRFSFNVKGGRCETCQGA
                     GIRLIEMNFLPDVRVLCETCQGKRYNRETLEVRFKGKSINDVLNMTIDQAVEFFENHP
                     SIIQKIKTLRDVGLGYITLGQQSTTLSGGEAQRVKLAAELSKRDTGQTIYILDEPTTG
                     LHFEDIKVLLNVLDRLVNRGNTVLVIEHNMDVIKMADHIIDLGPEGGIRGGEIVSEGT
                     PEKVAEDKNSFTGHFLLNELYDKRDYFCN"
BASE COUNT         1968 a         1180 c         1266 g         1914 t
ORIGIN      
        1 gcataaggtt caacacccac cgtttagcgt actgtgtgag atgtatagcc gttaaaaggc
       61 aaataggaac tgctattaac agcgaaacta ccgataccca caaggagctg atgatgaaag
      121 gataaaaacc aaatttgccc gaaaggggtt tccattgatt tgaaaaaagg atttgaaaaa
      181 aggattgttc gctcagtaaa aatgacgatt tatatatcaa accgctcaaa agagctacag
      241 gcactatcat agtaatagct aatactaata gcatccacga ataatgtagc ctatccctca
      301 ggtggcgacc aatattgaaa tttctcaacc tcatttaatc ttttgcatct cagtttcaat
      361 ttttgattcc gaaagacgta catagccggc atcattgacg tactgttgcc cttcggtaag
      421 tatccatttt aaaaattcaa ttacgatcgg gtttttaggt tcccctttcg atattagata
      481 tagatcacgg gctggcggtg aagggtattg ttcatttcca atagcggcac aaatgtcatc
      541 aagattgtcg taaaaattct catcagggtc tattaccccg ttattattca gatcaatcgg
      601 tattatttct aatccttcaa atttttttcg tgtatttata tcaaaggcat agataacatt
      661 gttgaagccg atcccgaatt tatcattttt aactgcatcg gctatgcctg ggtctccaaa
      721 tactcctatc ccttgaaggc tctcttgatt ttttccaaaa aaaagccccc acatttcagc
      781 ggcgccacag gcatcggaac gggtataaac atttatcttc tgattgttgg ctgagatacc
      841 cggtatctga tcccagtttt caatagttac atcaatgtaa atttttgtga gtgtctctct
      901 cgaaatgcca tactgcttaa tctctttaac gtatggattg gattcgttga tcgttcctaa
      961 tactgcatcc ttacataccg ccagatacca ggctcctttt tcaatttccg caggcgctat
     1021 ttcccttgac accattgcca gatcaaccat ttcggataat acatcggcca ttcctttacc
     1081 agcgccacct gcgctaatgt caatccgcac accaggattg agttgcataa actcctcagc
     1141 ccacttaacc gtcattggat aaagtgcaaa tgcacccgaa atggatatag tacccttaag
     1201 ctcacctttc ttgtgatgcc gattttctgg ctgcgaagta caaccattca gaatgaagta
     1261 aaaaaaataa aaagccgtaa taattagaag tgtaaatgtt gtttttgttc tcatgataat
     1321 aggtgttaga caacttttta ttctatgata ataatatttt tctgcaaagt taccccgtct
     1381 aacgccacca ttaacccctt taaaaactca atttttggaa atttatacct aattatcaaa
     1441 taagtattta tacgtattag gataattgag tagttttaat aatttattca aaaaaaagcg
     1501 ttttccgacc gttacgatgg ctgaatcacg aggtagtaaa agaaagagta gttaaaaagt
     1561 atgttattat ttttgctgaa cattcaaaca aactatattt gtctggttca ttaatcagct
     1621 ttttattaac tttttagtaa gatggatgtt caaattgaac tattaatttt aatactgtca
     1681 gttctttttt tctttagcat tcttgccggt aaagcgagtt ccaaatacgg tgttcctgca
     1741 ttattgctgt ttttgtcggt agggatgata tttgggagcg atggattagg cattcatttt
     1801 gagaatatac aggttgcacg taatcttggt gcgcttgctc tttgtgtaat actcttttcc
     1861 ggcggtcttg atacaaaaat tgacgagata cgccctgtaa tctttcaggg cgttattttg
     1921 gcaacaatag gagttttttt aacggcgctt ttcaccggca tttttgtctg gtggatttta
     1981 ggaatgacta tcaaatcggc cagtataggt ttgataacct ctttcctgtt agcctctaca
     2041 atgtcatcta ccgattctgc atcggtattt tctattttac gctccaaagg gctgcatctg
     2101 aaaaacaacc ttaggccaat gcttgagtta gagagcggaa gtaacgaccc aatggcatac
     2161 ctgttagtaa ttacacttat cgaattaata aacttcgaaa caacaccaaa ctattggatg
     2221 gtaagcttac aggtagtaat tcaattggtt gttggcgctt tgattggctt gactattggt
     2281 aagctgagca tatatcttat aaatagaata aaaatcggta acgattcgct ttacccgata
     2341 ttagttttaa caatctgttt atttattttc tctcttactt attttgcaaa agggaatggc
     2401 tatctggctg tttatgttgc tggtctgact attggaaacg gaaagtttgt ccacaaacgt
     2461 acttcgctta aattttttga tggtctggcc tggcttggtc agttaataat gttcttaacc
     2521 ttggggttat tagtgaatcc acgcgagcta gtaccaatta tgatcccagg tttgataatc
     2581 agccttgcaa tgatttttat tacccgccct ctcactgtct ttctctgctt gcttccattc
     2641 cgaaaaatgc caattagaga taaagcctac gtttcatggg taggcctcag aggagcggtg
     2701 cccattattt ttgcattatt tccacttacc gagaatgtac ctcatgcaag gcttatcttc
     2761 aacatcgtct ttttctgcac aataatttcg ttattagttc agggtacttc attacctgct
     2821 atcgccaaat ggctcgggct tacagaaaaa ccaccaaaaa ttggaaaaat tgaaagtttt
     2881 gatgttgatt tttctgatga tattaaatca atagttaccg aaattgagat aaattctacc
     2941 accctaagta aaggtaacaa agtgatggaa ttagagatac cggaagaaac acttgtggtg
     3001 atgataaacc gcgataacaa ttactttata cccaaaggga atacaatact aatggaaaaa
     3061 gacaaactat tgattattac cgttgatcat aattcgctac gcaaaacact aaaaaatctg
     3121 aatatacatg attctcaaag ctaattacag taaaaaaaac taagagactg aaagatgagg
     3181 attcaagtga aaatgaagaa ttaaattacc ctaaattaag tgttcgtcaa tgattatact
     3241 actaagcctc cgctttttgt tactattttt atcaaagttt acaattttca atgctttgca
     3301 ataaaaatgc ttttcatatt gtttagtgat tcgaattata attgaaattt tcatctccat
     3361 acatgagtaa cgaatccgcc acattggacc aaactcgtca gcctgaaggt tccaatttcc
     3421 ataatatgct tgaagtatat ggcgctagga tgcacaacct ccgcaatatt gatgtgaaga
     3481 taccccgcaa tagtttggta gtgattacag gccttagcgg cagcggcaaa tcctctttgg
     3541 catttgatac aatttatgcc gaagggcaga ggcgttatat ggagactttt tctgcttatg
     3601 cccgtcaatt tattggtaat atggagcgtc cggatgttga taaaattgat gggttaagtc
     3661 ctgtgatatc tattgaacaa aagagtgtaa acagaaaccc gcgttctacc gttggcacta
     3721 ttaccgagat ttatgatttt ttgcgcctgt tgtatgcccg aattggcgaa gcttattcga
     3781 taaatactgg agaaaagatg gtgcgtttta ccgagaagga gatcattgat cttattatga
     3841 gggagtatga aggtaagcaa ataaaccttt tagcgcccat ggtaaaagca cgtaaaggcc
     3901 actacagaga gctttttgag caaatcacaa agatgggtta cccgaaagtt agggtggatg
     3961 gcgagattag ggatatcacc ttcgggatga aggttgaccg ttataaaaca catgatattg
     4021 aagttgtgat tgacaggata cagcttgaag atagcatgaa tgaaaggata acaagatcgg
     4081 tacaggctgc tatgaaaata ggaaaagggg tgattatgat ccatgataca aaatccgatc
     4141 aggtcaggca ttttagcagt ctgttaatgt gcccgaccac cggtatctcc tatagtgagc
     4201 ccgagcctaa caccttctct tttaattctc cttatggagc ctgtccgcat tgcaacggac
     4261 ttggtgaggt gtcgcagatt gacttgaaaa aggtaatccc cgacgttaaa cgaaatatca
     4321 gaaatggcgg tcttgcacct ttaggtgagt ataaaaacaa ttggattttc aggcagatag
     4381 aggctatcgg ttataaatat ggatttaccc tcgatacacc aatcgaagat atccctgaaa
     4441 aaggaatgat aacaatatta tacggctctg atgaaattat tcacgtaaaa aacgaatatc
     4501 ttggcgttac ttctaactat tctctcaatt ttgaaggtat catcaatttc attctcaatc
     4561 aagattatga gacaagtagt aatcagataa aaaaatggat ttcaggcttt atgaaccgca
     4621 ttccatgtcc ggaatgtaat ggagccagac ttaaaaaaga gtcgttgcat tttaaaatct
     4681 tgaataaaaa tatcgctgaa ctggccaata tggatctgtt tcttttgcat ggctggattg
     4741 aaaatttacc atctcatgtc tctgaccgta cttttaaaat tgcaagagag attactcgtg
     4801 agatcagtac acgtatccga tttttactcg aagtaggctt agattacatc accttgaacc
     4861 ggtcggcaag aagcctttcc ggtggtgaat cgcagcgtat caggttggca acacaaattg
     4921 gctcgcagct tacgggagtt ctatatatac ttgatgagcc aagtattggt ctgcaccaac
     4981 gtgataaccg gcgtcttatc aatgccctga aacaacttcg cgacatagga aattcaatta
     5041 tcgtggtaga acacgacaag gagatgatac tctcgagcga ttacattgta gatattgggc
     5101 cgggcgcagg agtgaaaggg ggacttatag ttggacaagg caagcccgat agcatgaaag
     5161 attgtaatac cattacttgc gattacctga gtggaaaaaa aaggatatct gttccaaaaa
     5221 tcagaagatc aggcaatggt aagtttttga gattggaggg cgcaacaggg aataatctga
     5281 aaaatataac attgaatctg ccacttggca agtttgtgtg cattacagga gtgtcgggga
     5341 gtggcaaatc gagtttgatc aacgaaactt tatatccaat ccttagttct catttttacc
     5401 ggtcagaaaa aaagcctttg ccttataaaa agatatccgg tatcgagttt gttgataagg
     5461 ttattgagat tgatcagtcg cctattggcc gcacaccacg ctctaaccca gccacttata
     5521 ccaaggtatt tgacgaaata agaaaattgt atggctcatt acccgaatcg aagataaggg
     5581 gatatcaacc tggtaggttt tcgttcaatg ttaagggcgg acgatgcgaa acatgccaag
     5641 gagcaggaat caggttgata gagatgaatt ttttacccga tgtacgagtc ctttgcgaaa
     5701 cgtgtcaggg aaaaagatat aaccgtgaaa cacttgaggt caggtttaaa ggaaaatcca
     5761 taaatgatgt gctaaatatg acaattgatc aggctgttga gttttttgaa aatcatccct
     5821 ccattattca aaaaattaaa accctaagag atgttggatt aggatacatc accttaggac
     5881 aacaatcaac aaccctctca ggcggtgagg cacagcgggt aaaattggcc gccgaacttt
     5941 caaaacgcga taccggccaa acaatttaca tcctcgacga acctactacc ggcttgcatt
     6001 tcgaagatat taaagtgctg ctcaatgttt tggacaggtt agttaatcga ggcaacaccg
     6061 tactggtgat agagcacaat atggatgtaa taaaaatggc cgaccacatc attgatttgg
     6121 ggccggaagg cggaataaga gggggagaaa ttgttagtga aggaacacct gaaaaagtag
     6181 ctgaagataa aaattccttt accggccatt tcctattaaa tgaactttac gataaaagag
     6241 attatttttg caactaatta ttttataata aaaagaatat ggtaaacgac gaagcggatt
     6301 atcctatccg aaaagaagat tctgattc
//
LOCUS       DAOOAI010000110         6280 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_14572_length_6280_cov_5.630201, whole genome shotgun sequence.
ACCESSION   DAOOAI010000110 DAOOAI010000000
VERSION     DAOOAI010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14572_length_6280_cov_5.630201"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="PK345_07665"
     CDS             complement(<1..371)
                     /locus_tag="PK345_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HPX34928.1"
                     /translation="MKLLENKKALITGGSRGIGRAIAIAYAKQGADVAISDLMVDEDA
                     IDVVKEIESYGVKGKAYASDASSNEASESLINEVVKDFGQIDILVNNAGITRDNLLLR
                     MSEQDWDLVIKVNLKSIFNMT"
     gene            complement(410..2038)
                     /locus_tag="PK345_07670"
     CDS             complement(410..2038)
                     /locus_tag="PK345_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010535820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPX34929.1"
                     /translation="MKIGFHLHHIDNKLKFAFLIIFLYFNTFVFSQADKARKTTQKFS
                     TTLQLIEFGYVDSIDSPDLVEKAIIAMLKELDPHSAYIKAEDVKRLNEPLEAEFDGIG
                     VTFQLFNDTILVIAPVPGGPSDKLGILAGDKIIKINGEDAFGKKINNEYVMKLLRGKK
                     GTSVDVSILRSGKDELIDFTIVRDKIPINSIDATYMAAPEIGYIKLTRFAKSSVQEFN
                     ESLSRLRAQGMKDLILDLRGNTGGYLNIAVDLADQFLSDKKMIVYTEGLRSPKQEFFS
                     TGSGDFLRGKLIVLINESSASASEIVSGAVQDWDRGLVVGRRSFGKGLVQRPFNLPDG
                     AMIRLTTARYYTPSGRCIQKPYEHGETEEYYKDLTKRLESGELVDSNSIELADSLKYF
                     TSNGRVVYGGGGIIPDIFTPIDSTRFSDFYSDLVRKGIFNTYNLNYLEKNRKRVLKEY
                     PNVESFKEKFIVTDEMYADFLDLAKEKNVERKETEKYYYPDNDLKIQIKALIARNLWD
                     TNAYFRLINELDDELNVAVDLMQSGKAFTDLNLH"
     gene            2262..3743
                     /gene="rmuC"
                     /locus_tag="PK345_07675"
     CDS             2262..3743
                     /gene="rmuC"
                     /locus_tag="PK345_07675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HPX34930.1"
                     /translation="MTTTLLIAIIFLNILIVVLFFVFRPKKNDDFLKLIFKVGELQTE
                     LQKLENGLKEDFRINREESGRLNKENREELGAKFLEFSNQLISQLDKNFTQVQERMKE
                     VADRTKADNLQMREEVRKEIREFQNAFDKNVKSFNELQREKFGQLTEELRQKFNNLEE
                     KLKERLRLVENQQKELVETTEKKLEQMRNTVDEKLQKTLNERLSQSFETVGKQLQAVQ
                     EGLGEMKNLAQDVGGLKKVLSNVKIRGGIGEVQLSMLLEQMLAPEQYDANVVTKKGSS
                     ERVEFAIRLPGKEENQNVVYLPIDAKFPKETYEHLIDAFETGDPATIEIANRNMENVI
                     KKMAKDIRDKYIDPPNTTDFAIMFLPFEAIYAEVIRRSALFDQLHHEFKIIVTGPTTL
                     AAILNSLQMGFRTLVIQKRSSEVWNILSAVKTEFAKFGGMLDRAHKQITTAGQTIEQL
                     KGTRTNAINRKLRDVMALPNDESAEVFPELESADIDDEFEIEG"
     gene            3887..>6280
                     /locus_tag="PK345_07680"
     CDS             3887..>6280
                     /locus_tag="PK345_07680"
                     /inference="COORDINATES: protein motif:HMM:NF012700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HPX34931.1"
                     /translation="MIFPPNFEEKISFDHIRQMLVDMCVSSMGKEYVVKMRFETVFNR
                     IERKVSQVFEFLEILKYEQPFPAQNYFNLIPELQRIRLSGTYILQEQLFDLKSSLESL
                     NEVIVYLQKLDSAKYTLLSELVEDKRVDRVILNEIYSIIDEKGNIKDTASETLRDIRQ
                     RLIKLSGEIEKAVRQVLVTAKKSGWLNPNDEITIRNGRMVIPVPAANKRKIRGFVHDE
                     SATGQTVYIEPAEALDANNEILELRNAEKREIIRILTSFTDNLRPKIEELIEGYRFLG
                     LIDFIRAKALLAGKINGTKPFMSNEPLIDWRKALHPLLFLVHQKQKKHVEPLDILLNK
                     ENRILIISGPNAGGKSVCLKTTGLIQYMLQCGLLVPMDDNSKTGVFKDIFIDIGDEQS
                     IEDDLSTYTSHLRNMKHLALKANKNSLFLIDEFGSGTEPQLGGAIAEAILEHLNNKGA
                     FGVITTHYANLKHLAGKTPGLINGAMLFDTREMRPLYRLQIGNPGSSFAFEIAKKTGF
                     PWFILRNAERKIGKTQIKFEQQLQQLELEKKELTQKQQSLNEAEKQMNEAKSKYDELT
                     AKLIETKEKTLSDARSEAQRIIADSNKLVENTIREIKEAQAAKERTKKIREKLKVESE
                     RILAEPQKKSEAKPVEQTKAVIARSKKEKSAKEDTKILIGNRVRILPQQTVGEVIEIT
                     GGEAIVSFGSIMMRAKLEKLVNVGEEEFYQQVMIKKRGGENIFNELNTRMANFSVTLD
                     VRGMRAEEAATAVIQYMDEALLLNIKEVKIIHGKGDGILRKVVRDNLRSIDEVIHIED
                     "
BASE COUNT         2002 a         1156 c         1363 g         1759 t
ORIGIN      
        1 ttagtcatgt taaaaattga tttcaggttt accttgatta caaggtccca gtcttgttcc
       61 gacatgcgta acaaaagatt atcacgtgtt attccggcat tatttaccag aatatcaatc
      121 tgaccaaaat cttttacaac ttcgttgata agggactcgg aagcttcgtt agagcttgca
      181 tccgaggcgt aagcttttcc tttaacgcca taactctcaa tttctttaac cacatctatt
      241 gcatcttcat caaccattag gtcggagatg gcaacatcgg cgccttgttt tgcataagca
      301 atcgctatgg ctctgccaat accgcgtgaa ccaccggtta tcaaagcttt tttattttct
      361 aataacttca tttttgtgtg tgttaattag tgattattat attttaattt taatgtaggt
      421 ttaaatcagt aaatgctttg cccgactgca taagatcaac agcaacattc aattcgtcat
      481 caagctcgtt tatcagcctg aaataggcat tcgtgtccca caaattacgg gcaatcaaag
      541 ccttgatctg aattttgagg tcgttatcgg gatagtagta tttttcggtc tctttacgtt
      601 caacgttttt ctcttttgcc agatcgagga aatccgcgta catttcatct gttacaataa
      661 atttttcctt aaaactttca acattcggat actcttttaa tactctcttt ctgttttttt
      721 caaggtagtt taggttatat gtattaaaaa taccttttcg caccaagtcg gagtagaaat
      781 ctgaaaatct ggtcgagtca atgggagtga agatgtcggg tattattcca cctcctccat
      841 aaacaactct gccgttcgaa gtaaaatact tcaacgaatc ggctaactct attgagttgg
      901 aatctactaa ttcgccactt tcaagtcgtt ttgtcaggtc tttgtaatac tcttctgttt
      961 ctccatgctc gtatggcttc tgtatgcatc ggcctgatgg agtatagtat cgcgctgttg
     1021 tcagcctaat cattgctcca tcgggtaggt tgaagggacg ttgtactaac cctttaccaa
     1081 atgagcgccg gccaacaacc aatcctctgt cccagtcttg aacagcgcct gaaacaattt
     1141 cgcttgctga ggccgagctt tcgttgataa gtactataag ctttcctctc agaaaatcgc
     1201 cggagccggt ggagaaaaac tcttgctttg gcgaacgaag cccttcggtg taaacgatca
     1261 tttttttatc cgacagaaat tggtcggcca gatcaacagc aatgttaaga tacccacctg
     1321 tgtttcctct taaatcaagt ataagatctt tcatcccttg agccctaagt cttgaaagag
     1381 attcattaaa ctcttgcact gacgattttg caaaacgcgt tagcttaata tagccgattt
     1441 cgggagctgc catgtaggtg gcatcaatac tattgatagg tatcttgtct cttacaatgg
     1501 taaaatcaat taactcatct ttgccacttc ttaaaataga aacatcaaca gaggtgcctt
     1561 ttttacccct aagaagtttc ataacatact cattattaat cttcttaccg aatgcgtctt
     1621 caccgttgat ttttattatc ttatcaccgg ccagaatacc gagtttgtcc gaaggccctc
     1681 caggaacagg ggcaatgaca aggatagtat cgttgaataa ttgaaaggta actcctatac
     1741 catcaaattc cgcttccaac ggctcgttta atcgtttgac atcttctgct ttaatgtatg
     1801 ctgaatgagg atcaagctcc ttaagcatgg caataatggc tttttctaca agatcgggag
     1861 agtcaatact gtcaacatat ccaaattcaa tgagttgtaa cgtggtggaa aatttctggg
     1921 tagttttacg ggctttgtca gcttgtgaaa atacaaaagt gttgaaatac agaaaaataa
     1981 taagaaaagc aaatttcagc ttattatcaa tgtgatgtaa atgaaaacca attttcataa
     2041 ataattatga ttttaaacgt tcatctaatg ttaaacgtcc aaacgaaagc aaaatgaaaa
     2101 tatttttgct tagcaggaca atagctccta cgtcaaggaa gatttgatag aataacagcg
     2161 ttgatattga aatgtttacc gccttatttt gagataattg gttgatttag gatttttact
     2221 tgtagatttg ttacgagaaa tttttttaaa aacttacctg tatgaccaca actttactta
     2281 ttgccataat ttttttgaac atcctcattg ttgttttatt ctttgttttc aggcctaaga
     2341 aaaacgatga ctttttaaaa cttatcttta aagttggtga gcttcaaact gagttacaaa
     2401 agcttgaaaa tggtttgaaa gaggatttca ggatcaatcg ggaggagagc ggcaggctta
     2461 acaaagaaaa ccgtgaggaa ttgggtgcta aatttttgga gttttccaac cagttgattt
     2521 cacagttaga taaaaacttt acacaggttc aggaaagaat gaaggaggtt gccgatcgaa
     2581 ctaaggcgga caatttgcag atgcgagagg aggttaggaa ggagatcagg gagtttcaga
     2641 atgcttttga taaaaatgtt aaatcgttca acgaattgca acgcgagaaa ttcgggcagc
     2701 ttaccgagga gttaagacag aagtttaata atttggaaga gaagctgaaa gagaggttga
     2761 ggttagttga aaatcagcag aaagagttag ttgaaacgac tgagaaaaag ttagagcaaa
     2821 tgcgcaacac tgtagatgag aagctgcaga agacattgaa cgagcgtctt agccagtcgt
     2881 ttgaaactgt cggtaagcag ttgcaggctg tacaggaagg gttgggcgag atgaaaaatc
     2941 ttgcccaaga tgttggcggg cttaaaaagg tacttagcaa tgtgaagata cgtggaggaa
     3001 taggagaggt gcagctttcg atgctcctgg aacagatgct tgcccccgaa cagtatgatg
     3061 ccaacgtggt aaccaaaaag ggaagcagtg aacgtgttga gtttgccatc aggctgccag
     3121 gtaaagaaga gaatcaaaat gtagtctatc tccccattga tgctaaattt cctaaagaga
     3181 cctacgaaca tcttattgat gccttcgaaa ccggagatcc ggcaacaatt gaaattgcaa
     3241 accggaatat ggaaaatgta atcaaaaaaa tggctaaaga tattcgcgac aagtatatag
     3301 acccgccaaa tacaaccgac tttgctatta tgttcctccc cttcgaagcc atttatgccg
     3361 aggtaattag gcgttctgca ctttttgatc aactgcacca cgagtttaaa atcattgtaa
     3421 ccggcccgac aacccttgcc gctatactga atagcttgca gatgggtttt cgcacgctag
     3481 tcatccaaaa acgtagcagc gaagtgtgga atattctttc agcagtaaaa actgaatttg
     3541 caaaatttgg cggcatgctc gatagagctc ataaacaaat aactacagca gggcaaacca
     3601 tagagcagct caaaggaacg cgtacaaatg caataaaccg caaattaaga gatgtgatgg
     3661 cattgccaaa tgatgaatct gcagaggttt ttcccgaact cgaaagcgct gatattgatg
     3721 atgagtttga gatagaagga taacagactt gctgaataat agggtaaacc tcgttttaat
     3781 gtttgtattt tgcaattctc atttcgttgg attataaata tccaatcgtt taactattgt
     3841 caaattttta atctgggttt gtctattttt gtaaaaaata attatagtga tattcccacc
     3901 aaattttgaa gagaaaatca gtttcgatca tatccggcag atgcttgttg atatgtgtgt
     3961 aagcagtatg gggaaggagt atgtggtaaa aatgaggttc gaaacggtct ttaaccggat
     4021 agagcgaaaa gtgagtcagg tattcgaatt cttggaaatc ctgaagtacg aacaaccctt
     4081 tcccgctcaa aattatttca atttaatacc tgaattacaa cgtataaggc tgtcgggaac
     4141 ctatattttg caggaacagc tgttcgacct gaaaagctcc ctcgaatcgc tcaacgaggt
     4201 aattgtgtat ctacaaaagc ttgacagcgc caaatataca ttactctcgg agctggttga
     4261 ggataaaagg gttgataggg ttattctgaa cgaaatatac agtatcattg acgaaaaggg
     4321 gaatattaaa gatactgcat ccgagacttt gagagatata aggcaacgac ttattaagct
     4381 ttccggcgaa attgaaaagg cagtgcgcca ggttttagta actgcgaaaa agagtggctg
     4441 gcttaacccg aacgatgaaa taacaatccg caatgggagg atggttatcc ctgtgcctgc
     4501 cgccaataag cgaaagataa gaggttttgt acatgatgag tcggcaaccg gacaaacagt
     4561 atatattgag cctgccgaag cccttgatgc aaacaatgaa atactcgaac ttcggaacgc
     4621 tgaaaagcga gagatcattc gtattttaac ctcttttact gataatctgc gtccaaaaat
     4681 cgaagagctt attgaagggt atcggttttt aggattgatt gacttcatcc gtgctaaagc
     4741 ccttctggcc ggcaaaatca atggtacgaa gccatttatg agcaatgaac cgttgattga
     4801 ctggcgaaaa gcattgcatc cgttgttatt tctcgttcac cagaaacaga aaaaacatgt
     4861 cgaaccgctt gatatattgt tgaataaaga gaataggatt ttaataattt ccggacctaa
     4921 tgcaggtggt aagtcggtgt gcctgaaaac gaccggattg attcaatata tgctgcaatg
     4981 cggcctgctc gtccctatgg atgataattc caaaacgggt gtttttaaag atatttttat
     5041 agatatcggc gatgagcagt ctatcgaaga cgacctgagc acctatacct cacatttgcg
     5101 caatatgaaa catcttgcgc ttaaggcgaa caagaacagc ctcttcctga ttgatgagtt
     5161 tggtagcggc acagagccac aacttggagg cgccatcgct gaagctatcc tcgagcatct
     5221 gaataacaaa ggtgcatttg gagttattac tacccattat gccaatttaa aacatctggc
     5281 aggtaaaacg cccgggctta tcaatggcgc tatgctgttt gatacaagag agatgaggcc
     5341 gctataccgc ttacaaattg gaaacccagg cagctcattt gcttttgaaa ttgcaaaaaa
     5401 aacaggattc ccctggttta tccttcgaaa tgccgaacgt aaaatcggta agacgcaaat
     5461 aaagttcgaa cagcaactgc aacaactcga actcgaaaaa aaagaactga cacaaaaaca
     5521 acagagttta aatgaagctg aaaagcaaat gaatgaggca aagtcgaaat atgatgagct
     5581 taccgccaaa cttattgaga caaaagaaaa gacgctgagc gatgcccgtt ctgaggcgca
     5641 aaggataatt gctgatagca ataagttggt ggagaatacc attagggaga taaaagaggc
     5701 gcaggctgca aaggagagaa caaaaaagat aagagaaaag cttaaggttg agtccgaacg
     5761 gattttagct gagccgcaga agaagtcaga ggcgaaacct gttgagcaaa ctaaggccgt
     5821 aattgcccga tcgaaaaaag agaaaagcgc taaagaggat acgaaaattt taatagggaa
     5881 ccgtgtgcgt atcttgccac agcaaactgt tggcgaggtg atagaaataa caggaggcga
     5941 agcgattgtg agttttggca gtataatgat gcgtgcgaag ctcgaaaaat tagtaaatgt
     6001 gggagaggag gagttttatc aacaggttat gataaaaaag agaggcgggg agaatatttt
     6061 taatgaactt aatacacgaa tggcaaactt cagcgtaact ctcgatgtga gaggaatgcg
     6121 cgctgaagaa gctgcaacgg ctgtaattca atacatggat gaagcacttt tgctcaacat
     6181 aaaagaggta aagattatcc atggaaaagg tgatggcatt ttaaggaaag tggtaagaga
     6241 taacttacgc tcgattgacg aagtgattca tatagaagac
//
LOCUS       DAOOAI010000111         6028 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_15287_length_6028_cov_5.992634, whole genome shotgun sequence.
ACCESSION   DAOOAI010000111 DAOOAI010000000
VERSION     DAOOAI010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6028)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15287_length_6028_cov_5.992634"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..690)
                     /locus_tag="PK345_07685"
     CDS             complement(<1..690)
                     /locus_tag="PK345_07685"
                     /inference="COORDINATES: protein motif:HMM:NF025017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPX34932.1"
                     /translation="MINTNKNKRPKIFATLPALNESENIRQFIRDIMMQDYAEWNLIV
                     CVNQPEEWWDSPEKVSVCHDNALTLNYLQGINDKRIFLLDRSSKGNGWIGKRHGVGWA
                     RKVAMDFACTMAGNTDIILTLDADTHYPSNYFDSVINALESYPDATGLQAPYYHNLTG
                     NEQADRAILRYEIYMRSYAINLMAIRNPYCFSAIGSGMACSVEVYKRVGGLTPKMSGE
                     DFYFIQKLRKAG"
     gene            complement(795..2996)
                     /locus_tag="PK345_07690"
     CDS             complement(795..2996)
                     /locus_tag="PK345_07690"
                     /inference="COORDINATES: protein motif:HMM:NF013123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HPX34933.1"
                     /translation="MKKTLLLFVIVCLISSLQAQKEKELTLDLVFKSREFSAKRAQGL
                     QPLQDGKRYIQIKPDSINAYYYETGNLSEVIVTSDELIPAGDSTSIPIWGYELSGDES
                     KILFPTDTDPIYRYSSRSNYFVFDLKTRKLTQLSSNGKQGLADFSPDATKVAFVRDNN
                     LFIKNLNSNEEKQITFDGLDRHIINGTTDWVYEEEFAVTKGFHWSPDSKKIAFMRFDE
                     SEVKEWWLPTYGMLYPDYHSYKYPKAGEANSIVTIHVYDLETGTITLMDTGAETDQYI
                     PRILWTSNPENLAILRLNRLQNHLEILVADASSGESKVIYSEKNKYYINDNNYDDIIF
                     LDNDKEFILMSEKDGFNHIYLFDIKGEQKSQITQGNWEVTNLLGIDQKNRKVYYQSTE
                     KSPLERNIYVTGFDGKKKKELTPVKGWNSAQFSADFSFFIKTYSDANTPPVYSVNNGK
                     NGDQIRQLAENSKLIEKRNEYGFSPKEFFTIITSEGVELNAWKIMPHNFDSTKKYPVL
                     FSIYGGPGSQTVRNSFGGDMWEQYLAQEGIIVVSVDNRGTGFRGEEFKKMTYLQLGKY
                     ETIDQVEAAKYLGTLPYVDKNRIGVFGWSYGGYMSTLCLTKGAEIFSVAVAVAPVTNW
                     RFYDNIYTERFMRTPQENPDGYDDNSPINHVDKMQGKYLLVHGMADDNVHPENSYDLI
                     SALVAADKDFDLMLYPNSNHGIYTGYNTRFHLYKKITDFLMKNLKGVSEEE"
     gene            complement(3024..4418)
                     /locus_tag="PK345_07695"
     CDS             complement(3024..4418)
                     /locus_tag="PK345_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010800684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HPX34934.1"
                     /translation="MKAFSYSILTFFFIAILFLGCTNHQSKDQSYLFQPRKMFAIPER
                     EPGQTDVIELKCEPIDNVRIAIIGLGMRGSGAVRRLSYIEGATITVLCDVVPKNVEHS
                     QNILREMEKSEAVVYTGPDDWKEVCKHKDVDLVYVCTHWDLHTPIAVLAMENGKHVAV
                     EVPAALTVEECWQLVNTAEKTQRHCMMLENCNYDFFEMATLNMAQHGLFGEIVHVEGA
                     YIHDLRWLNFDDTTGYWDMWRLKHNERFDGNLYPTHGLGPIAHILNIHRGDKMDWLVS
                     VSSNQFGMSEYAREHFGKDSDYSIRDYKKGDMNTTIIKTHRGKTMMIQHDVTSPRPYS
                     RIHMVSGTKGFAQKWPRMGIALEPNGHYWMPDDEMKELLEEYKHPIVAEVGERAKEVG
                     GHGGMDFIMDYRLIYCLRNGLPLDQDVYDAAEWSSLVELTRISVANSGMPVQIPDFTR
                     GSWEKLKKVTYHSL"
     gene            complement(4538..5815)
                     /locus_tag="PK345_07700"
     CDS             complement(4538..5815)
                     /locus_tag="PK345_07700"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013610737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HPX34935.1"
                     /translation="MNYQQTLNFMFEKLPMYQRIGAAAYKSDLKNTLDLCRLLGNPEK
                     DFLSIHIGGTNGKGSTSNMIASVLQEAGYKTGLYTSPHLKTFRERIRVNGKMISEQEV
                     TDFINNHKEQIEAMYLSFFELTVGMAFNYFAKEQVDVAVVEVGLGGRLDSTNVITPLL
                     SVITNIGFDHMNFLGDTLKKIAFEKAGIIKQGVPVVIGQTQKEISNIFTDRANELKAP
                     VTFADQHWRVLKLAHNKIRIKHDSGFTVENFRFPLEGSFQQYNLCTVMEGIRILKENS
                     MFQISESSIEKGLANVIKNTGFSGRWHTIGSNPLTICDCGHNEDGIRQVLINISQTRH
                     NNLHMVFGMVNDKNREPILAMLPKEAQYYFCKPDIPRGLDAVVLAEDAKKFGLNGKVY
                     LSVKTALDAARKNARTNDLIFVGGSAFVVAEVV"
BASE COUNT         1739 a         1260 c         1086 g         1943 t
ORIGIN      
        1 accggccttt ctcagctttt gaataaaata aaaatcttcg ccgctcattt taggcgttaa
       61 tccaccaacc ctcttatata cctctacact acaagccatg cccgatccta ttgccgaaaa
      121 acaataggga ttacgtatag ccataagatt gatagcataa ctccgcatat aaatctcgta
      181 tcggagaatg gcgcgatcag cttgctcgtt acctgttaga ttatgataat agggcgcctg
      241 taagccggta gcatcaggat aactttccaa agcatttatt accgaatcaa aatagttaga
      301 aggataatgg gtgtcagcat cgagggtgag aataatatcg gtgtttccgg ccattgtaca
      361 tgcaaaatcc attgctacct tccttgccca gcctacgcca tgccttttgc caatccatcc
      421 gtttcctttg ctacttcgat caagcaggaa aatacgttta tcgtttattc cttgtaagta
      481 atttaaagtt aaagcattat catggcaaac gcttaccttt tcgggactat cccaccattc
      541 ctccggctga tttacacaaa cgataagatt ccactctgca taatcctgca tcataatatc
      601 tctaatgaat tgccggatat tttccgattc gttgagcgcc ggcaaagtgg caaagatctt
      661 aggtcgtttg tttttatttg tatttatcaa tttttgattg ccgaaaaaaa tattgtttaa
      721 acaaaatttg tttataacag ccttgaaatc atactttaag atttaaaaga gattattttt
      781 taagattgaa ttctttattc ttcttcggat actcctttca gatttttcat taggaaatcg
      841 gtaatttttt tataaagatg aaatcgggtg ttgtatccgg tataaattcc atgatttgag
      901 tttgggtata acataaggtc aaaatcctta tcggctgcta ctaatgccga gatgaggtcg
      961 tagctatttt ccggatgcac attatcgtca gccatgccgt gaacaagaag atatttccct
     1021 tgcattttat caacatgatt gataggtgag ttatcatcgt aaccatcagg attttcttgg
     1081 ggagtacgca tgaaacgttc ggtataaata ttatcgtaaa aacgccaatt agttaccgga
     1141 gcaactgcaa cagccacact gaatatctca gcgcctttgg taagacataa tgttgacata
     1201 taccctccgt aactccagcc aaatacgcca attcgatttt tatccacata aggcaatgtg
     1261 ccgaggtact tagcagcctc tacttgatca atggtttcat attttccaag ttggagatag
     1321 gtcatttttt taaattcttc accacgaaaa ccagttccac gattatcaac ggagactact
     1381 ataataccct cttgcgcaag gtattgttcc cacatatcgc cgccgaaaga gttccttacc
     1441 gtctgggagc caggcccacc gtaaatgctg aaaagaacag gatacttttt tgtagaatca
     1501 aaattatgtg gcattatctt ccatgcatta agctcgactc cttcagaagt aattattgtg
     1561 aaaaactcct tcggtgaaaa accatattca ttgcgttttt caataagctt agagttttcg
     1621 gctaattgcc tgatctgatc gccatttttt ccgttattca ccgaatagac gggtggtgta
     1681 tttgcatcgc tgtaagtttt tataaaaaag ctgaaatcgg cgctaaactg ggcgctattc
     1741 caccctttaa ccggggttaa ttcttttttc tttttaccat cgaaacctgt aacatatata
     1801 tttcgttcca atggtgattt ttctgttgat tgataataga cctttctatt tttctgatca
     1861 atgccaagca gattggtaac ttcccaatta ccttgtgtga tctgactttt ttgctcccct
     1921 tttatgtcaa aaagatagat atggttaaag ccatcttttt cactcatgag gataaactct
     1981 ttatcattat cgagaaatat tatatcgtcg taattgttgt cgtttatata atacttgttt
     2041 ttctcggagt atatcacttt tgattctcct gatgaagcat ctgccactaa tatttcaaga
     2101 tgattctgta gtcggtttag gcgtaggatt gccagatttt ccggattcga tgtccatagt
     2161 atacggggga tatactgatc cgtttcggcg ccggtatcca tcagtgttat cgtaccggtt
     2221 tcaaggtcgt aaacgtgtat ggtaacgata gaattagctt ctcctgcttt cgggtatttg
     2281 taactatggt aatcaggata taacatgcca taagtgggta accaccactc ttttacttcc
     2341 gactcatcaa agcgcatgaa tgcaattttt ttactatccg gcgaccaatg aaatcctttt
     2401 gttacagcaa actcctcctc ataaacccaa tctgtggtac cattgatgat gtgcctatcc
     2461 aatccatcga aagtaatttg tttctcctca ttactattta ggtttttgat aaagagattg
     2521 ttgtccctca caaaagctac cttggtggca tctggtgaga aatctgccaa gccttgcttg
     2581 ccatttgacg acaattgagt taattttctt gtcttcagat caaaaacaaa ataatttgat
     2641 cgtgatgaat accgatagat aggatcggta tcagttggga aaaggatttt gctttcgtcg
     2701 ccgcttagct catatcccca aataggtatt gaagtggaat cgccggcagg aatcaattcg
     2761 tctgaagtaa caattacttc cgacaaatta ccggtttcgt aatagtaagc attgatggaa
     2821 tcaggtttaa tctgaatata acgtttacca tcctgaagtg gctgtaaacc ctgtgccctt
     2881 ttagccgaaa actcccggga tttaaaaacg agatcaagcg tgagctcttt ttctttttgt
     2941 gcttgcaatg aactaatcaa gcagactata acaaataata acagtgtctt tttcatgttg
     3001 aacataatta ttgcattaaa aaattaaaga gagtgatatg ttaccttttt tagcttttcc
     3061 cacgatcctc ttgtaaaatc gggtatttgt actggcattc cactgtttgc taccgaaatt
     3121 cttgtcagtt caactaaaga ggaccattcg gcggcatcgt aaacgtcctg atcaaggggc
     3181 agtccattgc gcaggcagta aattagccgg taatccataa taaaatccat accgccatgg
     3241 ccaccaactt ctttcgcacg ttcacccact tcagcaacaa ttgggtgttt gtattcctct
     3301 aacaattcct tcatctcatc atccggcatc caataatgtc cgttaggttc gagggcaatt
     3361 cccatgcgtg gccatttttg cgcaaagcct ttagtgccgc ttaccatgtg gatacggcta
     3421 tagggccgcg ggctggttac atcgtgctga atcatcatgg ttttgccacg atgggtcttt
     3481 attatggtgg tgttcatatc accttttttg taatcccgaa tggaataatc tgaatccttt
     3541 ccaaagtgtt cacgtgcata ttccgacata ccaaactggt tactcgatac cgaaactaac
     3601 caatccatct tgtcgccacg atgtatattt aatatgtgag ctattggtcc taaaccatga
     3661 gtgggataga gatttccatc aaacctttcg ttgtgtttta atctccacat atcccaataa
     3721 cctgttgtat cgtcaaaatt cagccaccgc aggtcgtgga tataggcgcc ttcaacatgt
     3781 actatttcac caaaaaggcc atgttgagcc atgtttaaag tagccatttc gaagaaatcg
     3841 taattacagt tttcgagcat catacaatga cgttgtgttt tttctgcagt gtttacaagc
     3901 tgccagcact cctctaccgt taacgctgct ggaacctcta cagctacatg tttaccattt
     3961 tccatcgcca aaactgcaat aggcgtatgc aaatcccaat gtgtgcacac ataaaccaga
     4021 tcaacatctt tgtgtttaca aacctccttc cagtcatccg gcccggtata taccacagcc
     4081 tcggattttt ccatctctcg gagtatattc tgtgaatgtt ctacattttt aggtactaca
     4141 tcgcataaca cggtaatagt agcaccttca atatagctta accttctcac tgcacccgaa
     4201 cctctcatgc caagcccgat tattgcaata cgaacattat caataggctc gcacttaagc
     4261 tcaattacgt ccgtttggcc aggctcacgc tcaggtatcg caaacatctt acgaggttga
     4321 aaaagataag attggtcttt tgattggtga tttgtacagc ccaaaaacaa aatggctata
     4381 aaaaaaaatg tcagaataga ataagaaaat gctttcatgg aaaaaaaatt atccgttaat
     4441 tgtttgttta caaaaatata aatttgattt gggacttgag gtttttaaaa aaaattcgat
     4501 ttgtaaaatt taacaccaaa agccgatgtt taaatggtta tacaacttct gcaacaacaa
     4561 aagcgcttcc tccaacaaaa ataagatcgt tagtacgggc attttttcgg gcagcatcga
     4621 gtgcagtctt taccgagaga taaaccttgc cgtttagccc aaacttttta gcatcctcag
     4681 ccagcacaac ggcatcgagc cctcgtggta tatcaggttt acaaaaataa tattgtgcct
     4741 ctttgggaag catggcgaga ataggttctc tgtttttatc gttaaccatg ccaaagacca
     4801 tgtgtagatt gttgtgtctt gtttggctta tattaatcaa aacttgccgg ataccatctt
     4861 cattatggcc acaatcgcag attgtcaatg ggtttgagcc tatcgtatgc caacggcctg
     4921 aaaatcctgt gttttttatt acatttgcca atcccttttc aattgaactt tcggaaattt
     4981 gaaacatgga gttttctttc aaaatccgta ttccctccat aacagtgcag agattgtatt
     5041 gttggaaact accctctaac ggaaatcgaa aattttcgac agtaaaacct gagtcatgct
     5101 ttattctgat tttattatga gctaacttta gtactctcca gtgttgatct gcaaaggtaa
     5161 caggcgcttt aagctcattg gcccgatcag taaaaatatt gctgatctcc ttttgtgttt
     5221 gtccaattac cacaggcact ccctgtttta taataccggc tttctcaaaa gcaatcttct
     5281 ttaaagtatc tccaagaaaa ttcatgtggt caaagcctat attcgtaatt accgagagca
     5341 gtggggttat cacatttgtt gaatcgagcc gcccccctaa acccacctct acaacagcga
     5401 catcaacttg ttcttttgca aaatagttaa aggccatccc taccgtaagc tcaaaaaaag
     5461 aaagatacat agcttcaatc tgttctttgt gattgtttat gaagtcagta acttcctgtt
     5521 cggaaatcat ttttccatta acccgaatac gttctctgaa tgtttttaaa tgaggagaag
     5581 tgtaaagtcc tgttttatag ccagcctcct gcaatacaga tgcaatcatg tttgaggttg
     5641 aaccctttcc gtttgttcct ccaatgtgaa ttgataaaaa atctttctca ggatttccaa
     5701 ggagcctaca taaatctaac gtatttttta aatccgattt ataggctgca gctccaatac
     5761 gttggtacat tggtagcttt tcgaacataa aattgagggt ctgctgatag ttcatcacat
     5821 aaaattctgt tatggcagta aactaaagat ttttggtttt acatcaaaca aaaaactacg
     5881 gatagtggag gccaaaaata gtggttatca aaataatgag ttagattttt taaaaaaaat
     5941 caatataatg gtttgaatag tttagatttg cagaaaaaaa gagcgtatga ccttctcaat
     6001 cattgattac attgttttta caggttat
//
LOCUS       DAOOAI010000112         6014 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_15325_length_6014_cov_5.783521, whole genome shotgun sequence.
ACCESSION   DAOOAI010000112 DAOOAI010000000
VERSION     DAOOAI010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6014)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6014
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15325_length_6014_cov_5.783521"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="PK345_07705"
     CDS             <1..470
                     /locus_tag="PK345_07705"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34936.1"
                     /translation="DTVYYTGTIGCHRFVIYDSGGDGLTTYYTIRSYINGVMKTIGNG
                     SKFGYQEDKHFSVDSGVGVDEVNIEKSSVEIFPNPVLNISTVNFWLPEAGKATVDVYN
                     SAGKQVMQLANNNYYNAGTNSITLNSSNLDNGIYFISIKTSDQALIKKFAVMK"
     gene            complement(634..2724)
                     /gene="pta"
                     /locus_tag="PK345_07710"
     CDS             complement(634..2724)
                     /gene="pta"
                     /locus_tag="PK345_07710"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF004167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HPX34937.1"
                     /translation="MAQNLYIAASEEMSGKSLIVLGFMELLTRHVRRIGFFRPIVRND
                     KEEDSHIRLISNKFKLPFEYSSMYGITNKEAFEYIQSGEMESIFTRILAKYKQLEPQC
                     DFIVIEGTDFRGQLVSFEFDMNARIANNLGAPILAVINGFNKNSSQIADAIQMVRGTL
                     RSESCAQIGTVVNRAAEQEVEKIDKILSRYSKDGEISFVLPEKPVLQNITIRQTAKAL
                     GAKQVYGNEDSLNFDVQDLKIAAMSVDQVLKIITSGSLVITPGDRSDIIVGLLMAQIA
                     DTYPNIAGILLTGGQALNPQISKLLDGINQLPLSILSVDSDTYETALAVSNIRPALAP
                     ENERRLTTALSHFESNVNIDILGKKLKITRSKVITPIMFEYELFERARADRKHIVLPE
                     SGDERILRAAEILLKRNVVDITLLGNENEINKKAESLQIQLAGANIIDPHNNDRIHEY
                     ADEYFKLRKHKGITMEQAYELMYDVSYFGTMMVHKGHADGMVSGAANTTAHTIRPAFE
                     FVKTKPGVSIVSSSFLMCFSDRVLVYADCAVNPNPDAAQLADIAISSAETAINFGIEP
                     RIAMLSYSTGESGKGEEVEKVRKATHIAREQRPDLKIEGPIQYDAAIEPSVAQQKMPG
                     SDVAGKATVFIFPDLNTGNNTYKAVQRAANAVAIGPILQGLNKPVNDLSRGCTIKDIV
                     NTVVITAIQAQGGN"
     gene            complement(3046..4281)
                     /locus_tag="PK345_07715"
     CDS             complement(3046..4281)
                     /locus_tag="PK345_07715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34938.1"
                     /translation="MKRGLGCIFRLFLLLITLIIVVFFIVYAVAPVYEFPAPTPFSGK
                     VINNPYHKLDTALWRKGNFQVQSKVWMGLTDGRKNEAKAIYAVYHQLDYDVVAITDYM
                     KINRFEKDKEGYISAYEHGYGLWKTHQVCLGTNHITWLDYPFYQTLSHKQHILNTLKR
                     HCEVLAIVHPKVRNGYDLNDMKFLSNYDLLEALSHYAISLNYWDLALSSGHPVYIISN
                     DDSHDVLDPTKVGRYCTFFNSKSLQEDDVLSALKSGRAFGAYVNMLEGADFYQKAEDH
                     RNIPILQSVEVENDTLTVIVSEPAAAITFIGQNGIIRKIVSNSPTASYHISDNDQYIR
                     TEILFNNNTHFYLNPVIRHNGEGPDKPQAPNVNYTKTWLLRVFAFFIMLFFIRLIIKF
                     RKRNSTKRKKTFRQSFYNR"
     gene            4436..4951
                     /locus_tag="PK345_07720"
     CDS             4436..4951
                     /locus_tag="PK345_07720"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HPX34939.1"
                     /translation="MKQSKRVYRNSYDKVIAGVAGGLANYFNVDPIIIRLLFVALAFA
                     GAGGIIVYIILWIALPFNPDHDYYTFYNNQNPEDNQDEKTKNQDDTKSDEAEILDSNI
                     SARPFENTGKRDSKKEGNLIVGIFLIVLGLIFLIVRFIPDLYIRHFWPLILVIIGFVF
                     IYNALSKPKHQ"
     gene            4954..5886
                     /locus_tag="PK345_07725"
     CDS             4954..5886
                     /locus_tag="PK345_07725"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HPX34940.1"
                     /translation="MKSKDYFLGLLLVGLGIIFLLRNLNIFWFDWCHLKSLWPVIIII
                     VGIAFLPIHNALRIFLALIVVALSIFWISSKEPRDRSISGSKDSYWWKRDKDDDNDEK
                     CKWSKQILSEGYGSDISHAVLELDAIAGKYKIETTNEFLLKLEKEGNLGDYTLNTEAA
                     GNSKILRLKMKDRKTRTFNCTNNSKIFLNPMPIWDLNIDAGATSIDFDLEAFKIDKID
                     IDGGAASISLKLGDLNDKTDVRINAGAASITIEVPETSGCEIFTSTVISSKNFEGFEK
                     ESSGHYLTSNFDASEKKITLRIDAAVSGLKVKRY"
BASE COUNT         1800 a         1121 c         1126 g         1967 t
ORIGIN      
        1 gagatactgt atattatacc ggcacaatag gatgtcatcg ttttgtaatt tacgattccg
       61 gtggagatgg acttacaact tattacacaa taaggagtta tattaacggt gttatgaaaa
      121 ccattggaaa cggctcaaaa tttgggtacc aggaagataa acatttttct gttgattctg
      181 gagttggggt agatgaggtt aatattgaaa agagttcagt ggagattttt ccaaatccgg
      241 ttttaaacat atctacagtt aatttttggc tcccagaagc cggcaaggca acggtagatg
      301 tttataattc cgctggaaaa caagtaatgc agctggcaaa taataactac tacaatgccg
      361 gaacaaacag tattacgctt aattcaagta accttgataa tggtatttat ttcatcagca
      421 tcaagacatc agatcaagct cttatcaaaa agtttgctgt gatgaaataa ttaactgata
      481 tttttgaaaa tgactatttg ttagttgtca ttcatagaaa aaagtctaac tatattgtag
      541 ttaggctttt ttttattggc tattaacgac atccgccgta aggcagagat agatactgtt
      601 tcttgcttaa cctggcaagt gttaaaagcg attttagttt cctccctgtg cttgaatggc
      661 ggtgataacc acagtattta caatatcttt tattgtacat cctcggctca agtcatttac
      721 aggtttgttt agcccttgta atatcggtcc gatagctact gcatttgcag cgcgctgcac
      781 tgctttgtat gtgttgttac ctgtattaag gtcagggaag ataaaaaccg ttgctttgcc
      841 tgccacatca cttcccggca tcttttgttg agctaccgac ggttcgatag cagcatcata
      901 ttggattggc ccttcaattt ttaagtcggg acgctgctca cgagctatgt gagtagcttt
      961 tcttactttt tccacctctt cacctttacc cgactctccg gtagagtagg atagcatggc
     1021 aatgcgcggc tctattccaa aatttatagc ggtttcggcc gagctaatgg cgatatcggc
     1081 taactgcgcg gcatcaggat taggattcac agcgcaatcg gcatagacga gtacccggtc
     1141 gctaaaacac atcagaaatg agctggacac aattgaaact ccaggttttg tcttaacaaa
     1201 ttcaaatgca ggtcttattg tatgggctgt ggtatttgcc gcacccgata ccattccgtc
     1261 agcatgcccc ttatgtacca tcatagtgcc aaaatagctc acatcataca tcagctcata
     1321 agcctgctcc attgttatac ctttatgctt acgcaatttg aaatattcgt cagcatactc
     1381 atgaatccgg tcattgttgt gaggatcaat gatatttgca cccgcaagtt ggatctgcaa
     1441 ggattctgct tttttattta tctcattttc gttgcctaac aaggtaatat caacaacatt
     1501 acgtttcagc aaaatctcag cggctcttaa aattcgttca tctcccgatt ccggcaacac
     1561 aatatgtttt ctgtcggcac gggcacgttc gaataattcg tattcaaaca ttatgggggt
     1621 gataacttta gatcgggtaa tttttagttt tttacctaag atatcaatgt taacgttcga
     1681 ctcaaagtgg ctgagcgccg ttgtcagtcg tctttcattt tccggagcca atgctggtcg
     1741 tatattgcta acagccaatg ccgtttcata tgtgtccgaa tcaacagaca agatagatag
     1801 tggcaattga tttataccat cgagaagttt actaatttgt gggtttaggg cttgccctcc
     1861 ggtaagtagt attccggcaa tattagggta agtatcggca atttgtgcca ttagaagccc
     1921 gacaattata tccgaccgat cgcccggagt aattacaagg ctgccgcttg ttattatttt
     1981 taaaacctga tctaccgaca ttgcggctat tttcagatcc tgtacatcga aattcaaact
     2041 atcctcattc ccatacactt gctttgctcc cagcgctttg gcagtctggc ggatagtgat
     2101 attttgcaaa accggctttt cgggtaaaac aaagctgatc tcgccatctt tactatatct
     2161 ggagagtatt ttatcaatct tttcaacctc ctgttctgca gccctgttaa ccaccgttcc
     2221 tatctgagca caactctcgc tacgtagtgt gcctcttacc atttggatag catcagcaat
     2281 ctggcttgag tttttattaa aaccattaat aaccgctaat atcggtgcgc ctaagttatt
     2341 agctatacgg gcgttcatat caaactcaaa agagactaac tgccctctaa aatcagttcc
     2401 ttcgatcaca ataaaatcac actgtggctc caactgtttg tattttgcca aaattctggt
     2461 aaaaatactt tccatctcgc cggattgtat gtattcaaaa gcctccttgt ttgtaatacc
     2521 atacattgac gagtattcga agggaagttt aaatttgttt gaaataagcc ttatgtgact
     2581 atcctcttct ttgtcgtttc tcacaattgg tctaaaaaaa ccaatgcgtc ttacatgtct
     2641 tgttaaaagt tccataaacc cgaggacgat caatgatttc cccgacattt cctcagatgc
     2701 agcaatatat aggttctgtg ccattaattg tatttattat taagttaata gtaaatctta
     2761 aagtttgcaa aataacgaag tttatttgat ttcggcctcc ttttacgatg attttttatt
     2821 ccggattaat taattagaga acctttattg gccgtactgc tgattttcta ccgtttagtt
     2881 ggtggtttga gtaaagtttt gatttcatct catgggaatc agcattatta ttggttttag
     2941 gaatgaaatt tcgattttgc gtttgttaag aagatttatt atcctccgga taatggaaat
     3001 atctttgttt aagcaggtca gttggtaaca tttgggttta tccaatcatc tattataaaa
     3061 tgattgccgg aaggtttttt ttctttttgt agaatttctt tttctaaact tgattatcaa
     3121 tctgatgaaa aataacatga taaaaaaagc aaaaaccctg agcagccatg ttttggtata
     3181 gttaacatta ggcgcttgtg gcttatcagg tccctcgcca ttgtgcctga ttaccggatt
     3241 aagataaaaa tgagtattgt tattaaaaag tatctcagtg cggatgtatt gatcattgtc
     3301 agaaatatga tatgaagctg ttggactatt ggacacaatc tttcggatta ttccattttg
     3361 gccgataaaa gtgattgctg ctgcgggttc acttactatt actgtaagag tatcattctc
     3421 tacttccacc gattggagta ttgggatgtt tcgatgatct tccgcttttt ggtagaagtc
     3481 agcgccttcg agcatattaa cgtaagctcc aaaagccctg cctgacttta aagccgacaa
     3541 cacgtcatct tcttgaaggc tttttgagtt aaaaaaagtg caatatctgc ctaccttagt
     3601 aggatcaagt acatcatgcg agtcatcatt ggaaatgata taaaccggat gtccggatga
     3661 gagtgctaaa tcccagtaat taagcgatat cgcataatgg ctaagggctt ccaagagatc
     3721 ataattagac aaaaacttca tgtcatttaa atcatatcca tttcttacct taggatgaac
     3781 aatggctaat acttcacaat gccttttaag ggtattcagg atgtgttgtt tatgactgag
     3841 ggtttgataa aatggataat caagccaggt aatatgattt gttccaaggc atacctgatg
     3901 agttttccat aaaccataac catgctcata agctgagata tagccctctt tatccttttc
     3961 aaatctgttg atcttcatgt aatcggtaat ggctacaaca tcataatcta actgatgata
     4021 gacagcataa atcgccttcg cctcattttt tctcccatcg gttaatccca tccatacttt
     4081 cgactgtacc tgaaaattgc ctttccgcca taaagcagta tctaatttat gataagggtt
     4141 attgattact tttccggaaa atggagtagg ggcaggaaat tcataaacag gagcaacggc
     4201 ataaacaata aagaacacca ctataattag agtaatcaac aacagaaata atctaaatat
     4261 acagcctaat cctcttttca tcaggtttaa tgttgttatt ttagcctgct cttttaatca
     4321 cactaagcaa aaataaatat aaaaatgaaa ctaaggtgta actattttcg atttgtcaac
     4381 tctaatatca tgaaaattct tttttcttaa ctttgagcca aaaaataatt ttattatgaa
     4441 acaatctaaa cgagtttacc gcaattccta cgacaaggta attgcaggtg ttgcaggcgg
     4501 cttagccaat tattttaatg ttgatccaat catcatcaga cttctttttg ttgcgttggc
     4561 ttttgccggc gccggtggga taattgtata tataatcttg tggattgccc ttccattcaa
     4621 tcctgaccat gattattata ctttctacaa taaccaaaat cctgaagata accaagatga
     4681 gaaaacgaaa aatcaggatg atacaaagag tgatgaagcg gaaatacttg actcaaatat
     4741 ttctgcaagg ccttttgaaa ataccggaaa acgtgattca aaaaaggaag gaaaccttat
     4801 tgtcggtatt tttttaatag tattaggatt gattttttta atagtacgtt ttatcccaga
     4861 tctttatatt cgacatttct ggccgctaat acttgtcatt attggatttg tttttattta
     4921 taatgcttta tccaaaccaa aacatcaata acaatgaaat caaaagacta ttttttagga
     4981 ttattacttg tcgggcttgg gataatattt ttattacgca atttaaatat tttttggttc
     5041 gactggtgcc atttgaaatc gctctggcct gtcataatta taatagtagg tattgcgttt
     5101 ttgccgatac acaatgcgct aaggattttt cttgctctga ttgtagtagc cttatcaatt
     5161 ttttggattt cctctaaaga gccaagagat aggtctatat ccggatcgaa agatagctat
     5221 tggtggaaac gcgataaaga tgatgataat gatgagaaat gtaaatggag caaacaaatc
     5281 ttaagcgaag gttatggctc tgatatcagc catgcagtac tcgaattgga tgcaatcgca
     5341 ggaaaatata aaattgagac aacgaatgaa tttttactaa aacttgaaaa agaaggaaac
     5401 cttggcgatt atactcttaa cacggaagcg gctggtaatt caaagatatt acgactgaaa
     5461 atgaaagatc gaaagacgag aactttcaat tgtacaaata actctaaaat attcctgaat
     5521 cctatgccta tttgggattt aaatattgat gcaggcgcca catctataga ttttgatttg
     5581 gaagctttta aaattgataa aattgatatt gatggaggtg cggcttccat ttcactcaaa
     5641 ttgggtgatt tgaacgataa aaccgatgtt aggattaatg ccggagcggc ttcaattacg
     5701 atagaggtgc cagagacttc cggatgtgaa atattcactt caactgtaat aagctcaaaa
     5761 aattttgaag gctttgaaaa agagagttca ggccattatc tgacatcaaa cttcgatgcc
     5821 tcagaaaaga aaataaccct acgaattgat gctgctgtat caggacttaa ggttaaaagg
     5881 tattgattgc cgagtctatt atccgtaatt aacatagttg tcataactcc ggcaaatcct
     5941 gctattaagc cgatttttac tgataagctt cgttcttagt ttcgaatact tcatagtaaa
     6001 caattaccca atcg
//
LOCUS       DAOOAI010000113         5747 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_16147_length_5747_cov_6.357519, whole genome shotgun sequence.
ACCESSION   DAOOAI010000113 DAOOAI010000000
VERSION     DAOOAI010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5747)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5747
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16147_length_5747_cov_6.357519"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(77..424)
                     /locus_tag="PK345_07730"
     CDS             complement(77..424)
                     /locus_tag="PK345_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34941.1"
                     /translation="MNPNFNQTLLQTDVLFTENDLSAITGFETITGISDDTFTIRISI
                     YPNPTSGVINVIGLDANAKINILDAKGQMLLTDSGIPSDLITIDLSSHQPGVYFIRIE
                     INEQHIYRKLVLI"
     gene            complement(447..2156)
                     /locus_tag="PK345_07735"
     CDS             complement(447..2156)
                     /locus_tag="PK345_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34942.1"
                     /translation="MKKIIPITTLLLLVCMALFAQTVNIKAVSTSTRFAGSASFEPGP
                     LMNVPRMGHVAITLPDGRTGFFGGHTTNFVALNTSEIWTPGNTTPVTLTMNYYHSWPV
                     FVLLNDGRYLLAGGCSNIGIPKYATSEIFNPSNTSFTAVGNMVRFRSSMNSGGTAMSD
                     GKVLIAGAWWTHNNAHTYGELFNPNTNNFSAVGPFEAARSHVVVMPTGDGKAVVVGGR
                     TATGNSTQQIMELYDPGTGSISTIQTELFSDDPDWVVSSDLRPTGVQKMEDGRYIWLA
                     YKIIDAVFHYRLLVFDPATKTISHFETIPPLPTSEASTRFYRHPVVDIERNRIHLMAT
                     SSGASYPIITIFSVDLSTGILIQLENSFNPGYYIDGADINLLQDGRLMISGGSSANTN
                     FTPVRNTLLIDLEFTSIPSQVALIPQGWSGLSSYIIPNQPAIEDVFAPISNELIIAQT
                     MNEMFYPGQNINTIGNWVSHSAYKIKTNSACSLSIVGAFETDMSVSLNAGWNLLPVVT
                     PNDVDAANLLTFVNGFVIAKDVAGTGVFWPQYNINTIGNLLPGKAYYVMMTAPGVVDY
                     TKD"
     gene            complement(2609..4891)
                     /locus_tag="PK345_07740"
     CDS             complement(2609..4891)
                     /locus_tag="PK345_07740"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005635576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HPX34943.1"
                     /translation="MKKLILFLLIIIGLPVTSQNTGNDLIHYVNPFIGTQRMGHTFPG
                     ATVPFGMVQLSPDTDTVSYELNGSYNPRVYEYCAGYQYDDPTIVGFSHTHFSGTGHSD
                     LGDFLIMPTTGKLQLNPGTANNPESGYRSRFSHENEFAEPNYYSVLLEDYSITAELTT
                     SARVGFHRYTFPENAEVNIILDLMHGIYNYDGKNVWTFVRVENDTLVTGYRQTNGWAR
                     TRTVYFAMTFDRPITKYGHKKYDKTVYRGFYRKFNEGENFPEMAGHDIRAYFNFDLDE
                     TCELKIKFALSNVSAAGASKNLAAEVPHWDFDKTKREGQQLWNNELSKIRVEMLDNDD
                     STVFYTALYHSFLSPIIYEDVDGQYRGLDQNIHQSDGFTNYTIFSLWDTYRALHPLFN
                     LVQPCRNRDMVKSMLAHYDQSVHKMLPVWSHYANENWCMIGYHSVSVIADAIAKGIYN
                     ENLPKALEACKSTASYPLYDGLNYYLKLGYVPEDKSSNSVSKTLEYAYDDWAIAQIAL
                     RVGDLSAYDNFMQRSESYRHVFDSVSGFMRPKLSDGNFREAFDPLDTHGQGFIEGNAW
                     NYGLYIPHDPEKLVKLNGGKAAFAAKLDSLFVLELDDAHIEANEDITRDGIIGLYVHG
                     NEPGHHIPYLYNWTGQPWKTQERVRMIMNTMYANAPDGLCGNDDCGQMSAWYIFSALG
                     FYPVCPGSEEYALGSPKVVSAEVDLGNGKILSIKIENQHRENVFVREVWLNERRIEGY
                     TLNHHDLAKGGTLKFVMSNR"
     gene            5272..5565
                     /locus_tag="PK345_07745"
     CDS             5272..5565
                     /locus_tag="PK345_07745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34944.1"
                     /translation="MKKLNFHYSLHRKIIPDEGRKPLDIHEMTFYFNNLIDIIQTWMN
                     SSQNTENKKYDPLSIINSPMFGNGEQKRIYHEPEIHFKKRGIICYTPIIIPER"
BASE COUNT         1725 a         1208 c         1157 g         1657 t
ORIGIN      
        1 taattgaata tctacagttt atttttttca ataagaaaaa ggggacaagt caggaaacat
       61 aacttgtaat cttcgcttat atcaggacta atttcctgta aatgtgttgt tcgttaattt
      121 cgatcctgat aaaataaacc cccggctggt ggctgctaag atcaatggtt atcaggtctg
      181 acggaatgcc ggaatccgtc agaagcattt ggccttttgc atcaagtatg ttgatttttg
      241 cattagcatc cagtccgata acattgatca ctccggaagt cggattggga taaatgctaa
      301 ttcttatcgt aaaagtgtcg tcgctgatcc ctgtaattgt ttcaaatccg gtaattgccg
      361 aaaggtcatt ttcggtgaat agaacgtcag tttgtaggag agtttggttg aagttgggat
      421 tcaaacctgt caggtttccg ctactcttaa tcctttgtat aatccaccac tccgggagct
      481 gtcatcatta cataatacgc ttttccggga agcaagtttc cgatggtatt gatattgtac
      541 tgtggccaga agacaccggt tccggcaaca tctttggcta taacaaaacc attgacaaac
      601 gttaataaat ttgcagcgtc aacgtcattc ggagtaacaa caggcagcag gttccaacca
      661 gcgttgagcg aaacgctcat gtcggtttca aatgcaccaa caatagatag gctgcatgcc
      721 gaatttgttt ttattttgta cgccgaatgg cttacccagt tgccaatagt atttatgttc
      781 tgtccgggat agaacatttc attcatggtt tgggcgatga tcagctcatt ggaaattggt
      841 gcaaaaacat cttctatagc cggctggttg gggattatat agcttgaaag cccgctccag
      901 ccttgtggga taagcgctac ttgtgatggt attgaagtga actcaagatc tatcaacaaa
      961 gtattacgta caggtgtaaa attggtattt gccgaactac ctccactaat cattaatcgt
     1021 ccatcctgca aaagattaat atctgcgcca tcaatgtaat atcctggatt aaaagagttt
     1081 tcaagctgta taagtatgcc cgttgataaa tctacactaa aaatagtaat tatagggtaa
     1141 gatgctcctg agcttgtagc cattaaatgg attctgttgc gttcgatgtc aacgacagga
     1201 tgcctgtaaa atctagtaga agcttcactt gtgggcaaag gtggtatagt ttcaaaatga
     1261 gaaatagttt ttgttgctgg atcaaaaacc aataaacggt aatggaatac tgcatcaatg
     1321 attttatagg caagccaaat gtatctgccg tcttccattt tttgaacacc tgtcgggcgt
     1381 aaatctgatg aaacgaccca atccggatca tcactaaata attctgtttg aatggtagaa
     1441 atactacctg ttcccgggtc atataattcc ataatttgtt gcgttgaatt accagtagct
     1501 gtgcgaccgc ctacaactac ggccttccca tcaccggttg gcattacaac tacatgagac
     1561 cgtgcagctt caaaaggtcc aacagccgaa aaattgtttg tattggggtt aaacaattct
     1621 ccataggtat gtgcattatt atgcgtccac caggcgcctg caatcaacac tttgccgtcg
     1681 gacattgccg taccgcccga attcatggaa gagcggaatc gcaccatatt accaacagcg
     1741 gtaaaagagg tgttggaagg attaaaaatc tcgctggtgg catatttagg aataccaata
     1801 ttggaacaac caccggcaag caaatatctg ccatcattca ataatacaaa gactggccag
     1861 ctatgatagt aattcatggt aagtgttaca ggagttgtat ttccgggcgt ccatatttcg
     1921 gaagtattta aagcaacaaa atttgttgta tgacctccaa aaaatcctgt acgaccatca
     1981 ggtaaagtaa ttgcaacatg acccatacgg ggcacattca tcaacggacc aggttcaaaa
     2041 gaggctgaac cggcaaatcg ggttgaagta cttacggctt taatatttac cgtttgcgca
     2101 aaaagagcca tacatactaa taataagagc gttgtgatcg gtattatttt cttcatgttg
     2161 ataaagtttt aataatttta attgagataa atattcctaa tttttttaag taaaagtaaa
     2221 aacaaatttt ggtttacctc aataaaaaat gcaataatgt ttataaaata aaattacgct
     2281 agttttaatt tactgaatat taaaagtata gctccttaac taaaacgaaa tttagagaga
     2341 tactaaatta gaaaactaat cctgctttca ggccggccga ctgcgaaaaa ccaagcgttt
     2401 ggagaatttt tttgaaagtt ttaaaaaatc attttacaaa acaatgacat caaatcagaa
     2461 aaactatttg aaacttcaaa ggctaatcac tcatcacaaa acttagcatg gcaactttca
     2521 ttaaaacact gttgaagtaa gacacgactt tcaattcttc ttccgtgaag ctaaaaatac
     2581 cctttcagcg ttgccgccag cctgcttttt atctattgct cataacaaat ttgagcgttc
     2641 cccctttggc cagatcatgg tgatttagtg tgtaaccttc aatccttctt tcatttagcc
     2701 aaacctccct aacaaataca ttttcacgat gttggttttc aatttttatg gagagaattt
     2761 ttccattacc taaatcaacc tcagccgaaa ccaccttcgg actccctaat gcatactcct
     2821 ccgatcccgg gcaaaccgga taaaatccca acgcactgaa gatataccac gccgacatct
     2881 gcccgcagtc gtcattgccg cagagaccat cgggggcgtt ggcatacatc gtattcataa
     2941 tcattcgcac ccgctcctgt gttttccagg gctggccagt ccaattataa aggtaaggga
     3001 tatgatgacc cggctcgttg ccatgcacat acaatccgat gatgccgtcg cgggtgatgt
     3061 cttcgttggc ttcgatatgg gcatcatcaa gctcaaggac aaacagtgag tcgagtttgg
     3121 cagcaaaagc tgccttgcca ccatttagtt tcactaattt ttcagggtca tgcgggatgt
     3181 aaagtccgta attccaggca ttcccttcta tgaagccctg cccgtgcgtg tcaagtggat
     3241 cgaaagcctc ccgaaaattc ccatcagaaa gcttcggacg cataaaacca ctcaccgaat
     3301 caaacacatg ccggtagctc tccgagcgtt gcatgaaatt gtcgtaagcg ctcaaatcgc
     3361 caaccctgag cgcaatctgt gctattgccc agtcatcata ggcatactca agagttttgc
     3421 tcaccgaatt actgcttttg tcctccggca cataacctaa ctttaagtaa taattcaggc
     3481 catcgtaaag agggtaagag gcagtagatt tgcaggcctc aagcgctttg ggaagatttt
     3541 cgttatagat acctttggca atggcatcag caatcaccga aacgctgtga taaccaatca
     3601 tgcaccagtt ttcgttagca taatgcgacc acaccggcaa catcttatgc acactctgat
     3661 cgtaatgcgc cagcatggat ttcaccatat cgcggttgcg acagggttgg acaaggttga
     3721 acaacggatg cagcgcccgg taggtgtccc acagcgaaaa gatagtataa ttggtaaaac
     3781 catccgattg atgaatattt tgatctaatc cgcggtactg cccatcaaca tcttcataaa
     3841 taattggcga cagaaaacta tggtaaagcg ctgtgtaaaa aacagttgag tcgtcattgt
     3901 ccaacatttc cactcggatt ttcgaaagct cattgttcca tagctgctgc ccttcacgtt
     3961 ttgttttatc gaaatcccag tgagggactt cggcggccag gtttttcgaa gctccggcag
     4021 cgctcacgtt ggaaagtgca aatttgattt tcagctcaca tgtttcgtcc aagtcaaaat
     4081 tgaaataagc cctgatgtcg tgtccggcca tttccggaaa attttcacct tcgttgaatt
     4141 tacggtaaaa cccgcgataa acggtcttgt catacttttt atggccatat ttggtaatcg
     4201 ggcggtcgaa agtcatggca aaatagactg tccgcgtacg cgcccagccg ttggtctgac
     4261 ggtatccggt tactaacgta tcattttcaa cccgaacaaa cgtccagaca tttttgccat
     4321 cataattgta aatgccgtgc atcaggtcga ggattatgtt gacttcggca ttttcgggaa
     4381 aagtatagcg gtgaaaccca acccgagcac tggtggtcag ttcggcagtg atgctgtaat
     4441 cttcgagcag aacgctgtaa tagtttggtt cggcaaattc gttttcgtgc gagaacctcg
     4501 aacggtagcc cgattccggg ttgttggcag taccgggatt aagctgcagt tttccggtgg
     4561 ttggcatgat caggaaatcc ccgagatcgg aatggcctgt tccgctgaaa tgggtgtgac
     4621 tgaaaccgac gatagtcggg tcgtcgtact gataaccggc gcaatactca tacacacgcg
     4681 gattgtagct gccattcaac tcgtaactga ccgtgtcggt gtccgggctt aactgcacca
     4741 tcccaaaagg caccgtagcg ccggggaaag tatgccccat ccgctgggtt ccgatgaacg
     4801 ggttgacgta atgaattaag tcattgcctg tgttctggct ggtaaccggt aatccgatga
     4861 tgattagcag aaagagaatt aattttttca ttgttaggaa tttggttcaa aaccttgttc
     4921 atgctataaa atcgcaaaat taaccacaat aggcacaata ggctagagta agaatttttt
     4981 ttaattttct aatgcgctct attgtgcata gtgtagcaaa aaacagacaa aatcaaaagt
     5041 acccattttt tttggtgatc gggtattcat aaatggaatc atagtctcga tatcattttc
     5101 agaaatgtac aatgatatag aataatataa aatatgtagc gctaaataac actacgtaaa
     5161 cactctgccc ccctaatttt tttaaatcaa tttctcctta aaaaattctg gttttgaaag
     5221 gttttattat atttgacgcc aatattttac acacaaaaat tgaatctaga aatgaaaaaa
     5281 ctaaattttc attacagcct ccacaggaaa ataattccgg acgaaggccg caaaccatta
     5341 gacattcatg aaatgacatt ttattttaat aatttaatag atatcattca aacgtggatg
     5401 aattcaagcc aaaataccga gaataaaaaa tatgatccat tatccataat taacagccct
     5461 atgtttggta atggtgaaca aaaacgcatt tatcatgaac ctgaaattca tttcaaaaaa
     5521 agaggcatta tttgttatac accaataatt atacctgaac gatgaaacga caatttttta
     5581 taatcctttt ctgtttgacg ccgatttatt attcatggtc gcaaaacacc ttcaattttt
     5641 ttttgaagta ccatgttaat aaatggacac aaaatgcagt ggaggataac aacggaaatt
     5701 gaacccggct gcggccatca atgcattgat tttgtctcta atttgac
//
LOCUS       DAOOAI010000114         5642 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_16512_length_5642_cov_4.782531, whole genome shotgun sequence.
ACCESSION   DAOOAI010000114 DAOOAI010000000
VERSION     DAOOAI010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5642
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16512_length_5642_cov_4.782531"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2863
                     /locus_tag="PK345_07750"
     CDS             <1..2863
                     /locus_tag="PK345_07750"
                     /inference="COORDINATES: protein motif:HMM:TIGR04056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0071702 - organic substance transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SusC/RagA family TonB-linked outer membrane
                     protein"
                     /protein_id="HPX34945.1"
                     /translation="TIIDANDKSTIPGASILIKGTVMGTTSDINGVFHLSVKPNTTLV
                     ISFVGYKTQEVVVQPNTNIEVILNFEVTALEELVVIGYGVQKKSDRTGAVQSITAAEM
                     NSGVLTDPIQGLQSKIAGVMVSKQGGDPNSGFDIKIRGASSLVTNTSPLYVIDGVPGA
                     DPTTIAPEDIETFNILKDASAAAIYGSRGANGVVIITTKRGTERKGAQIDFNTFFSLE
                     KVAHRLDLVSGEDYRKFISEYPALGTSFIDGGANTDWQDQIFRTGSSQNYNLAFSGGD
                     SKTSYRASLSHQIFNGLVIESDKTRTIGRINLDQKALNDRLTISTGMSATIENNNYIS
                     YSGWGRNEVLFQAYQRNPTDPLRDSAGQFYEIERVFQYYNPVALVDQIHNEREAKRYF
                     GYLKADYEIIPGLFAGVNMAYTRNDDESFYFEPSTMYIGTHEGYGRRGYYNFDSKVLE
                     TTLRYNTDFGKNSIQTVLGYSFQEDMQDGFSAQGRQPFNNYTMMHDLTLFQSVNPGDI
                     SSFKNTNRLISFFARGIYNYDSKYFFTATLRRDGSSKFGINNEWGWFPSASLMWNITH
                     EQFMQNIDFINSLRLRVGYGITGNQEIGNYNDIAYYQAAGNSFNFETGENAILFQYAH
                     NANPNLKWEENAELNIGIDYGFLHDRISGSIDYFKKNTYDLLGNYSVPVPPNPVDRIW
                     ANVGEFEVSGIELFLQGYPVRNNTFDWKTSIVFSSYKQDVISLSNETYEWSQMQVGYL
                     SGPGLVGDLNWTQIVRPGTELGTWYMPQFAGFSQDGKFLFYTESGGVTRELEKAERRI
                     VGSAQPDFELGWSNYFTYKKNWDINFTFRGVFGHEVFNTTRLVFGNPIFLPDRNVLYE
                     ALDEYRRGLRDNPKLSDYYLEDGTFVRLDNISIGYNFSNVRGFERIRLYFASNNVFTI
                     TGYTGIDPEISPSGLSFGLDQYNTYPKARTYTLGLNITL"
     gene            2883..4382
                     /locus_tag="PK345_07755"
     CDS             2883..4382
                     /locus_tag="PK345_07755"
                     /inference="COORDINATES: protein motif:HMM:NF019592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RagB/SusD family nutrient uptake outer membrane
                     protein"
                     /protein_id="HPX34946.1"
                     /translation="MKKIFLLFIVLSLIQFGCTDLDDVLYDKIPADEYVADPILKMSP
                     IYEPMRDFCDWGGWWFAQELTGDAVVCPTRGGDWDDGGKWRVLHQHTWDNNTEAINSM
                     WSRFYRGVVEANKFIEEQEVFAGDSIVDEAIAKALILRSYYFYLLIDNYKDVPYTTQY
                     IGAPEQPKRNFRKDIYNEILKTVEECAPMISSSTSKTGVNPGMVYTLLAKLYLNHAVY
                     LDEVKPEYWEKAELACKEVIDLGQYSLESDPLAPFVTNNENSSENIWTIPYDEDHYEG
                     FNLHMRTLHYNSNQTFEMVVGPWNGFAVIYDHYLTYEDNDRRKEGFLVGQQYTYNNQP
                     IMDLGAGGIPLIFNPVIPSLVMGEGNTPEEIRNSGARVVKFEVKRGAKANLSNDFPIF
                     RYADVLLMKAEALIRQGKNGDEEVNKIRERAGLSTWSNVTLTQLLAERGRELFWEAHR
                     RQDLIRFDEFNKPWWEKQASTADRKTFPIPQWAIEANPNLALPPVSLND"
     gene            4460..>5642
                     /locus_tag="PK345_07760"
     CDS             4460..>5642
                     /locus_tag="PK345_07760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34947.1"
                     /translation="MKSNSFKWLLRAAALFLVSAVIFVSCKKDKDDPDPQIQVEDGIY
                     IVGNGTALTDYNIKGLLKSTRNEVNQSDRSELMEIYIAVKAGSDGFNIKKVAGATRIT
                     YGPGSDFTVVGEEERIGDEPQLDFWRGSYVESATPFTVPADGLYHVVIDTEVGKVAVI
                     PVQFWGLIGAATSGGWSNDTKIFPKGFDLNTMTFEADNITMTKADFKFRYSSGWKVVI
                     DDDYDLGGGVKGIRVNTNYGGAVNALVPGGANIDNETGGIYTAKMVWTLGEEYAATLT
                     KTGDLQVFDYSNTELGLVGESLIVNGAPHNWEETVFLTLPTVENETNYTWTFADVEVQ
                     AGGGGFKIREGQNWEGKIIGYPQVTMAGIAANNFETDGDGNFVPIDAGVFDFELFINA
                     VT"
BASE COUNT         1706 a         1151 c         1226 g         1559 t
ORIGIN      
        1 aactattatt gatgccaatg ataaatccac cattcctggc gcttcaatat tgatcaaagg
       61 cactgtaatg ggtacaacaa gcgacattaa cggtgttttt cacctttctg taaagccaaa
      121 caccacttta gttatctctt tcgtcggtta taagactcaa gaggttgtgg tacagcctaa
      181 cactaatatt gaggtaatcc tcaactttga agtaacggcc ttggaggagc ttgttgtaat
      241 tggctacggt gttcagaaaa agtctgaccg taccggcgct gtccaatcta tcactgcggc
      301 agagatgaac agtggcgtat tgaccgatcc tatacaagga ttacaaagta aaatcgccgg
      361 tgtgatggtg tcgaaacaag gcggcgaccc taactcaggc ttcgacatca aaatcagagg
      421 ggcatccagt cttgtaacta ataccagtcc tctttacgtg attgatggcg ttcctggagc
      481 cgatcctact accatagcgc cagaagatat tgaaactttt aatatcctga aagatgcttc
      541 tgccgcagct atttatggct cacgaggcgc taatggagtg gttatcatta ctaccaaaag
      601 gggaacggag agaaaaggag cacagattga cttcaacacc ttcttctccc tcgaaaaagt
      661 ggctcaccgc ctcgatttag tgagtggcga ggattaccgc aaattcattt cagaatatcc
      721 ggcgctcgga acctcattta ttgatggtgg cgcaaatacc gactggcagg atcaaatttt
      781 ccgcaccggt tcatcgcaaa actacaactt agctttctct ggcggcgaca gtaagacaag
      841 ctacagagct tcgctgtcgc atcagatttt taacggattg gtgatcgaat cggacaaaac
      901 ccgcaccatc ggccgtatca acctcgacca aaaagcgctt aacgaccgcc ttactatctc
      961 aaccggcatg tcggccacca ttgaaaataa taactatatc agctatagcg gatggggacg
     1021 caacgaagtg ctgtttcagg cttatcaacg taatcctacc gatccactcc gcgacagcgc
     1081 cggacagttt tacgagatag aacgtgtgtt tcagtattac aatccggtag cgcttgttga
     1141 tcaaattcac aacgagcgtg aagctaaaag atactttgga tatctaaaag ctgattatga
     1201 aattatccca ggtctgtttg ccggagtgaa tatggcctat acacgtaacg acgacgagtc
     1261 gttttatttt gagccatcaa cgatgtatat tgggactcat gaaggttatg gacgcagagg
     1321 atattataat ttcgactcca aagtgttaga gactacattg cgttataata ctgactttgg
     1381 taaaaactca atacaaaccg tattgggtta ttcctttcag gaagatatgc aagacggatt
     1441 ttcagcacaa ggacgtcaac cttttaacaa ctacacgatg atgcacgacc tgacgctgtt
     1501 ccaaagtgta aacccgggcg atatatcttc ttttaaaaac accaacaggc tcatctcctt
     1561 ttttgcaaga ggcatttaca attatgattc aaagtatttc ttcacagcta ctttacgtcg
     1621 cgacggctca tccaagtttg gaattaataa tgagtggggt tggttcccgt ctgcctcgtt
     1681 gatgtggaac attacccacg agcagtttat gcaaaatatt gattttataa acagcttacg
     1741 tctgcgtgta ggatatggta ttacaggaaa tcaggagatt ggaaactata acgatattgc
     1801 ctattatcag gctgccggaa actctttcaa cttcgaaacc ggtgaaaatg ccatcctttt
     1861 tcaatatgcg cacaatgcca accccaacct caaatgggag gagaacgccg agttgaacat
     1921 aggtattgat tatggttttc tgcacgaccg tatctccggc tctattgatt attttaaaaa
     1981 gaacacctac gaccttcttg gaaactattc cgtgcccgtt ccacccaatc ctgttgaccg
     2041 tatatgggca aacgtagggg agtttgaagt atcagggatt gagttgttcc ttcaaggcta
     2101 tcctgttaga aacaatactt tcgattggaa aacatcaatt gtgttctcat catataaaca
     2161 agatgtgatc tcgctttcca acgaaaccta cgaatggtcg caaatgcagg taggctatct
     2221 ctcggggcca gggcttgttg gcgatcttaa ctggacacaa atagtgcgtc cgggaactga
     2281 gcttggaacg tggtacatgc cacaatttgc cggtttctcg caagacggca aattcctctt
     2341 ttacaccgaa tcaggagggg taacacgaga acttgaaaaa gccgaaagaa gaattgttgg
     2401 atcagcacag ccggatttcg aacttggatg gtctaactac tttacctaca agaaaaactg
     2461 ggacatcaac tttacttttc gcggagtatt tggacacgag gttttcaata caactcgtct
     2521 tgtttttgga aaccccatct ttttacctga cagaaacgtc ctatatgaag ctttagatga
     2581 gtacagacgc ggccttcgcg ataatcctaa actaagcgat tactatcttg aagacggcac
     2641 ctttgtacgc ttagataaca tttcaatagg atataacttt agcaacgtca gaggttttga
     2701 aaggatacgc ctttactttg cctccaacaa tgtgtttaca ataacaggat atacaggcat
     2761 tgatcctgaa atatctccca gcggcctctc ttttggcctt gaccaataca acacatatcc
     2821 aaaagcacgc acttatactt taggccttaa cattacttta taatcttaaa aatcattaga
     2881 aaatgaaaaa aatatttctt ttattcatag ttttatcatt gattcaattc ggttgtaccg
     2941 accttgacga tgttttatac gacaaaatac ctgccgatga atatgttgca gatccaattt
     3001 taaagatgag tcccatttat gagccgatga gggatttctg tgattggggt ggctggtggt
     3061 ttgctcaaga attaaccggc gatgctgttg tatgccctac ccgtggtggc gactgggatg
     3121 atggcggtaa gtggcgcgtg cttcatcagc atacctggga caacaacacc gaggctatca
     3181 atagtatgtg gagccgcttt tatcgaggag tggtagaagc taataagttt attgaagaac
     3241 aggaggtttt tgccggcgat tcaatagtgg atgaagcaat tgcaaaagca ttaatactca
     3301 gatcctatta tttctatctc ctgattgata actataagga cgtaccttat accacgcaat
     3361 atatcggagc gcccgaacag cctaaaagaa atttccgcaa ggatatatac aacgaaatat
     3421 tgaaaactgt agaggagtgt gcgcctatga tcagttcaag cacctcaaaa acaggcgtaa
     3481 accccggcat ggtttatact cttctggcta aactctacct taaccatgcc gtctatttag
     3541 atgaggttaa acctgaatat tgggaaaaag ccgaattagc ttgtaaagaa gtaattgatc
     3601 ttggtcagta ttcgcttgaa agtgatccac ttgctccatt tgtaacgaat aatgaaaact
     3661 catctgaaaa tatttggact ataccttatg atgaagatca ctatgaggga tttaatcttc
     3721 acatgcgtac actacactac aacagtaatc aaacttttga gatggttgta ggaccatgga
     3781 atggatttgc cgtaatctac gatcactatt taacgtatga agataacgat agacgcaagg
     3841 aagggttcct tgtcgggcag caatatactt acaacaatca acctataatg gatctgggcg
     3901 caggcggcat accgctgata ttcaaccctg ttatcccttc attagtaatg ggcgaaggca
     3961 atacaccgga ggagatcaga aatagcggcg cacgggtagt aaaatttgaa gtcaagcgcg
     4021 gagctaaagc taatttaagc aacgattttc caattttcag atatgccgat gtgttgctta
     4081 tgaaagctga ggcgttaata cgtcaaggga agaatggcga tgaagaggta aataaaattc
     4141 gtgaaagggc agggttatcc acatggtcga atgttaccct tacacaactt cttgctgaga
     4201 gagggcgtga attattttgg gaggctcatc gtcgtcagga tcttatccgc ttcgatgaat
     4261 tcaataaacc gtggtgggaa aaacaagcaa gcacagccga cagaaaaaca ttcccgatcc
     4321 cgcaatgggc tattgaagct aacccaaatc tcgcattacc gcccgtttct ttaaatgatt
     4381 aacaataaat taatactttt gtagtttaaa aatcaaattt ataacatcta acttaattct
     4441 taatcaaaac tatctaaaaa tgaaaagtaa ctcatttaaa tggttattac gtgcagcagc
     4501 gctatttttg gtctctgctg taatttttgt atcttgtaaa aaagataagg acgaccctga
     4561 tcctcaaatt caggttgagg atggtattta cattgttgga aacggcactg cccttaccga
     4621 ctataacatt aaaggtttat tgaaatctac ccgtaacgaa gtcaatcaat cagacagatc
     4681 tgagctaatg gaaatctata ttgcagtaaa agctggttct gacggcttca atattaagaa
     4741 agttgcaggc gctacaagaa ttacttatgg ccctggtagc gactttacag tagtaggtga
     4801 agaagagaga attggtgatg agccacaact tgacttctgg cgtgggtcct atgttgaatc
     4861 tgcgactcct tttacagtac ctgccgatgg tctataccat gttgtaatag atactgaagt
     4921 agggaaagta gctgttattc ctgtacaatt ctggggattg attggcgctg ctacttcagg
     4981 tggatggagt aacgacacta aaatatttcc taaaggcttc gatctcaaca caatgacatt
     5041 tgaagctgat aatattacaa tgacaaaagc tgatttcaaa ttccgctatt cctctggttg
     5101 gaaggttgtc attgatgacg attatgatct cggtggtggt gtaaaaggca taagagtcaa
     5161 tactaattac ggtggagcag tgaatgcatt agtacctgga ggcgctaata ttgataatga
     5221 aaccggcggg atatacaccg ctaaaatggt ttggacatta ggcgaagagt atgcagctac
     5281 tttaactaaa acaggagacc tccaagtctt tgattatagc aataccgaat taggattggt
     5341 aggcgaaagc cttatagtaa acggcgcacc acataactgg gaagaaaccg tatttttgac
     5401 actgcctact gttgaaaatg aaactaacta tacctggaca tttgcagatg tcgaagtaca
     5461 ggctggtggc ggtggattca agataagaga aggtcagaac tgggaaggca aaataatagg
     5521 ctatcctcaa gttacaatgg ctggaattgc tgctaataac tttgagaccg acggcgatgg
     5581 taacttcgtt cctatagacg ccggcgtttt tgatttcgag ctattcatca acgctgtaac
     5641 cg
//
LOCUS       DAOOAI010000115         5629 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_16566_length_5629_cov_4.615716, whole genome shotgun sequence.
ACCESSION   DAOOAI010000115 DAOOAI010000000
VERSION     DAOOAI010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5629)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5629
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16566_length_5629_cov_4.615716"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            93..3062
                     /locus_tag="PK345_07765"
     CDS             93..3062
                     /locus_tag="PK345_07765"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPX34948.1"
                     /translation="MKQFFILLVLLTNSLTLFSQERLIIRFTNPDPVIVKHFASNNYD
                     IAAFHPGVFLDLVVTEDHYKELLSQGFEAEIFQTESEMRANLTSKADNIPGYRGYVQA
                     LADLQLLEATYPDICKLYDIGDSRGKVYYNEGKQNYVNYQHDIWALKVSDNVELDEDE
                     PAIYYMGAHHAREPLSTEVAFYVLDHIIENYGVDPVITENVNSKEIWFIPIVNPDGQK
                     IVLDQTDTNWRKNIRDNDNNNQITFSNNGWSYPDGVDINRNYGWQFGTEGTSFDPTDV
                     TYCGPSKFSEPETSAIRDLMASRHFVAGISYHTYSELVLWPYGYTTNAIGPDNAAISA
                     LGTAMGNSIPKLGSGTYTPQPSWALYPSSGVTDDYAYGEHGIFSYTIELGTQFIPPAS
                     QILQICQANIQAAMILLNRVNTSTLTGHITNSNTGEPVVAEIYVDGIDNTGLYRKPYK
                     SNENFGRYYRFLPNGNYNVTISAFGYITQTFQNININNVGQTILDVQLVPCQSFTVTG
                     TVTDIDTGEPIANASIVVIDTPLPTFYTDENGNYTIPSIFENTYSFKIWALNYATLIQ
                     EITINAQNLQFDFELTETNAVSFETGVFPEGWTFGGNANWTIVNTAAWDGEYSARSGA
                     IGNNQKSEMKFTVIAANAGVISFYVKVSSEANYDFLRFYVNEQQKGAWSGELNWTEVS
                     IPVNAGTNNFKWVYQKDIYVSSGSDCAWVDYIIFPPSATVNANAGPDTEICADETHQC
                     QGTASFYNSVTWSTSGDGQFSNASSLTPIYTPGTTDISNGGVTLSLTAYGDGGTSKTD
                     QMILTIKPLPGTGTQITGIDEVCAGNSETYFCEIIDDADEYEWNIQPSNAGQITIQND
                     NEIMVEWNINYEGEAILTVRGTNECGYGDYSDDFMVTILDCSYIDEMDNQNATIYPNP
                     AKDQIIIYPNPNMEGVLSLKIFNNLGVVIHKANYQYKATENIVVDLKNTISGVYYVQI
                     NTERHSFIKKLIINKDM"
     gene            3177..>5629
                     /locus_tag="PK345_07770"
     CDS             3177..>5629
                     /locus_tag="PK345_07770"
                     /inference="COORDINATES: protein motif:HMM:NF039776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GEVED domain-containing protein"
                     /protein_id="HPX34949.1"
                     /translation="MKKNNLLLLLVLLIALIPFNIKAQRKLSLDDGEYRNDVQVKMGE
                     LNPYEVLDTRIDNMRYWRKAAALGLTPIEPYHEVGLGQYKGNRINAKSVVRDDSPDVP
                     VTKVNSTQSENSIFINPNDTEHVLQSNNSTQNPVGQLYGANYFYSFDFGQTWGGSIQG
                     AGGTNSGDPTTAINLNGRQFVGYIHSNGGQGVSYSDNGTTWTPVICAYAGGGGLLDKN
                     HLWVDNSATSPFVGNLYNAWTDFYGPNNNNIEIIRSTNAGVSWTNKQNISSAVNAGSH
                     NQGVNIQTGPNGEVYAIWSIYDSWPSDEKAIGFAKSMDGGVTFSTATRIINNIKGIRT
                     TEVGKNHRVNSFPSMATDISGGAYDGNIYIVWANIGVPGVNSGTSTDIYMIRSTNQGQ
                     TWSTPIKVNQDPTGLGKKHYFPWITCDPETGALSVIFYDDRNVTSNKCEVWCANSFDG
                     GDTWEDFRVSDVDFTPAPISGLAGGYMGDYLGIAARGAKVYPVWTDNRTGTAMTYVSP
                     YETNNLPRPFDLLATIIFETGEAQLTWQFENVPSFQYFIIYRDNVQIATTTNLQFSDY
                     LPTYGVFKYVVTAMHDEGESSGTSTIVQWGDAHISVDPEEIIENIVLDTTSTRYITVE
                     NTGQLDLIYEVSGSTQPIREITDYCIPSGNCSWGDGITGFSMGDIVNMNNGCSPGAYG
                     NFTNMSTEIQPGQVYSVSMSTGYSNNFATIWIDFNKNETFESSEIVLSGFALQNSNQV
                     YTTNITIPDGVESGETRLRIKAQWSSTPNDPCASMNYGETEDYTVNVCGWMSVQRVTD
                     TIAPGNTKIIAVNFDSSDL"
BASE COUNT         1800 a         1145 c         1150 g         1534 t
ORIGIN      
        1 cgaatgacta tctgatttta aatggcctct tttgtgtttc aaaatagtat tttaaaacta
       61 aatcttattt atctcacatt ttatctttta tcatgaaaca gttttttatt ctattagtgt
      121 tgttaactaa ctcgttaact ctcttttctc aggaacgatt gatcatccgt tttaccaatc
      181 ccgatccggt tattgtaaag cattttgcta gtaataatta tgatatcgca gccttccatc
      241 ccggcgtttt tcttgatctc gtagtaactg aagatcacta taaggagttg ttatcgcaag
      301 gttttgaagc cgaaatattt caaaccgaat cggagatgcg tgcaaacctc acatcaaagg
      361 cggacaacat tccaggatac aggggttatg tgcaggcgct tgccgactta caactcttgg
      421 aagctaccta tccggatatt tgtaagctat atgatatcgg cgatagccga ggaaaggtat
      481 attataatga aggaaagcaa aattatgtaa actatcagca cgacatctgg gctttaaaag
      541 tatcggataa tgtggagctt gatgaagatg aacctgctat atactacatg ggcgcacatc
      601 atgcccgcga gccgcttagc accgaagtag cattttacgt tttagatcat attattgaaa
      661 actatggcgt agatccggta attactgaaa atgtaaattc aaaagagata tggtttatac
      721 caattgtaaa tcccgacggc cagaaaattg ttttggatca aacagacacc aattggcgta
      781 aaaatatacg cgacaacgat aataacaatc agattacctt ttctaataac ggatggagtt
      841 atcctgatgg cgttgatata aatcgtaact atggatggca gtttggcacc gaaggcacct
      901 ctttcgatcc gacagatgtt acctattgtg ggccgtcaaa attttccgaa cctgaaacct
      961 ctgcaattcg cgatcttatg gcatcacgac attttgttgc aggtatttct tatcatactt
     1021 acagcgagct tgtattatgg ccttacggct atactaccaa cgctataggc cctgataatg
     1081 ctgctatctc agcgcttgga acagccatgg gcaactcaat cccaaagtta ggctccggaa
     1141 catatacccc tcaaccgtca tgggcgctat atccttcctc aggtgttacc gacgactacg
     1201 cctacggaga acatgggata ttcagctata ccatcgagct gggcacacag tttataccac
     1261 ctgcaagtca gatattacaa atttgccaag ccaacattca ggcagcaatg atacttctca
     1321 acagagttaa cacttcaact cttaccggac atattaccaa ctcaaacacc ggcgagccgg
     1381 tagtagcaga aatatatgtt gatggaattg ataataccgg actttataga aaaccctaca
     1441 aaagcaatga aaattttgga agatattatc ggtttctccc aaatgggaac tataacgtta
     1501 ccatttcagc ttttggatat ataacccaga cctttcaaaa cataaatatt aataatgtcg
     1561 gacagacgat tttggatgtt cagcttgtac catgtcaatc ttttacagta accggaacag
     1621 ttaccgatat tgataccgga gaaccgattg ctaacgcctc cattgtagtt attgacactc
     1681 cgttaccaac tttttatacg gatgagaatg gtaattatac tattccttca atttttgaaa
     1741 acacttactc atttaagata tgggcgctga attatgccac cctaatacaa gaaatcacca
     1801 ttaacgccca aaatcttcaa tttgattttg aactaaccga aacgaatgcc gtcagttttg
     1861 aaacgggtgt gtttcccgaa ggatggacct ttggcggtaa tgcaaattgg actattgtaa
     1921 acactgcagc atgggatggc gagtattctg ccagatcggg cgccataggt aacaaccaga
     1981 aatcagagat gaagtttacc gttatagcag ctaatgcggg agttatttct ttttatgtaa
     2041 aagtatcatc agaagccaac tacgatttcc ttaggtttta tgttaacgag caacaaaagg
     2101 gagcatggtc aggcgagtta aactggacag aggtatcaat acctgtgaat gccggtacaa
     2161 acaattttaa atgggtatat caaaaagata tatatgtctc ctccggctcc gattgcgcat
     2221 gggtagatta tatcattttc ccgccctcag ccacagtaaa tgcaaatgcc ggtccagata
     2281 cggaaatctg tgctgatgag acacaccaat gtcagggaac ggcatctttt tacaattcgg
     2341 taacatggtc aacatcaggg gatggtcagt tttccaacgc ctccagcttg actcccattt
     2401 acacgccagg cacaacagac attagcaacg gtggtgttac actttcatta acagcctatg
     2461 gcgatggagg aacatcaaaa accgatcaga tgatcttaac aataaaaccg ctaccaggca
     2521 caggaacaca aatcactgga atagatgaag tttgcgccgg aaatagtgaa acatattttt
     2581 gcgaaataat tgatgacgct gatgagtacg agtggaatat tcaaccttca aatgccggcc
     2641 aaataacaat tcaaaacgat aatgaaataa tggtagaatg gaatattaac tatgaaggcg
     2701 aagcaatttt aactgttaga ggaacgaatg aatgtggtta tggcgactat tctgatgatt
     2761 ttatggttac aattcttgat tgctcctata ttgatgaaat ggataatcaa aatgcaacaa
     2821 tttatcctaa tccggcaaaa gatcaaatca tcatttatcc taatccgaac atggaaggag
     2881 tattatcctt aaaaatattc aataatcttg gggtggttat ccacaaagct aattatcaat
     2941 ataaagcaac ggaaaacata gtagttgatc ttaaaaatac tatttcaggt gtctattatg
     3001 ttcagataaa taccgaacga cacagtttca tcaaaaaact cattatcaat aaggatatgt
     3061 aaaaggcttg tgggttaact tgttttatgg aacttttaat aaagatatat ttttattttt
     3121 gcagaattgt tttaaacaat atcactataa cgtattaaca aacattaaat taagctatga
     3181 aaaaaaataa cttgttattg ctattagttc tcttaatcgc tttaataccc ttcaatatta
     3241 aagcccagcg taaattatca ttagatgatg gcgaatacag aaatgatgta caagtaaaga
     3301 tgggtgagct aaacccttac gaagtacttg atacccgtat tgataatatg agatattggc
     3361 gcaaagccgc tgccttaggt ttaactccga tcgagcctta tcatgaggtt gggttagggc
     3421 aatataaggg caaccggatc aatgcaaaaa gcgttgtcag agatgattct cccgatgtgc
     3481 ctgttactaa ggttaactca acacaaagtg aaaactccat ttttatcaat cctaatgaca
     3541 ctgaacacgt tcttcagtca aacaactcta cacaaaatcc ggtcggtcag ctttatggcg
     3601 caaactattt ctattcattt gacttcgggc aaacctgggg aggctcaatt cagggagcgg
     3661 gtggtaccaa ctccggcgat cctacaacag cgatcaacct gaatggaaga cagtttgtag
     3721 ggtatattca ttccaacggc gggcaagggg tatcatactc cgacaatggt actacatgga
     3781 ctccggtaat atgtgcctat gccggtggcg gcggcttgct cgataaaaat catctgtggg
     3841 ttgacaacag cgctacaagc ccctttgttg gcaatttgta taacgcatgg actgactttt
     3901 acgggccaaa caacaacaat attgaaataa tcagatcaac caatgctggg gtaagttgga
     3961 caaataaaca aaatatcagc tcggcagtga acgccggaag tcataatcag ggagtaaata
     4021 ttcaaacagg ccccaatgga gaagtatatg caatttggtc aatttatgat agctggccat
     4081 ccgatgaaaa agccatcggc tttgcaaagt caatggacgg tggagtaact ttctctacag
     4141 ccacacgaat catcaataat atcaaaggaa tacgaactac cgaggtggga aaaaatcata
     4201 gagtaaactc atttccatct atggctacgg atatcagcgg cggcgcttac gatggaaata
     4261 tatacattgt ctgggctaat attggtgtac caggtgttaa ttccggtact tcaaccgata
     4321 tttacatgat ccgttccacc aatcaggggc agacgtggtc aacgcctata aaggtgaatc
     4381 aagacccaac gggattgggt aaaaaacact atttcccatg gatcacctgc gatcctgaaa
     4441 caggtgcgct aagtgtcata ttttatgacg atagaaacgt aacttccaac aaatgtgagg
     4501 tatggtgcgc caactcattt gatggtggcg atacatggga agatttccgt gttagcgatg
     4561 ttgatttcac accagcccct atttccggcc tcgccggagg atatatgggc gactatctcg
     4621 gcattgcagc acgaggagca aaggtttatc ccgtttggac cgacaaccgt acaggaacag
     4681 caatgaccta tgtttcgccg tatgaaacca acaacctacc acgaccattc gatttgttgg
     4741 ctacaattat attcgaaaca ggtgaagcac aattgacatg gcaattcgaa aatgttccgt
     4801 cattccaata ttttatcata tatcgggata atgttcaaat agcaacgaca acaaacttgc
     4861 aattctccga ttatctgcca acatacggag tgtttaaata cgtcgtaacg gcaatgcacg
     4921 acgaaggcga gtccagcgga acaagcacaa ttgtgcagtg gggtgatgca catattagcg
     4981 ttgacccgga agaaatcatc gaaaatattg tgttggatac cacttcaaca cgatatataa
     5041 ctgttgaaaa taccggacaa ttagatctga tttacgaggt gtcaggctca acacagccaa
     5101 tccgtgagat tacggattat tgcattcctt cagggaattg ctcttggggc gatggcataa
     5161 caggcttttc aatgggcgat atagtaaaca tgaacaatgg ctgcagccca ggagcttacg
     5221 gcaattttac caacatgagc accgaaatac agcccgggca agtttattca gtaagcatgt
     5281 caacaggata tagcaataat ttcgctacca tttggattga cttcaacaaa aatgaaacgt
     5341 ttgagtcctc ggaaatagtg ctcagtggtt tcgctcttca aaattcaaat caggtttaca
     5401 ctacaaatat taccatacct gatggcgtag aaagtggtga aacacggcta aggataaagg
     5461 cacaatggtc aagcactccc aacgaccctt gcgcctcaat gaattatgga gaaaccgaag
     5521 attacacagt aaatgtgtgt ggctggatgt cggtacaaag ggtaaccgac acgatagcgc
     5581 caggcaacac taaaatcatt gccgtcaatt tcgattcaag cgatctgga
//
LOCUS       DAOOAI010000116         5627 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_16574_length_5627_cov_5.051687, whole genome shotgun sequence.
ACCESSION   DAOOAI010000116 DAOOAI010000000
VERSION     DAOOAI010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5627)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5627)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5627
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_16574_length_5627_cov_5.051687"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..519)
                     /locus_tag="PK345_07775"
     CDS             complement(<1..519)
                     /locus_tag="PK345_07775"
                     /inference="COORDINATES: protein motif:HMM:NF018054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HPX34950.1"
                     /translation="MNKWTKLSIEYANQRSYLDDLFQVYPTIPEGIREINKDIWAEVE
                     KSFKKKDNDLLIRQLLKLDLFPIKDSYIAYLKRDSSAIDRNPRTINRICGRLYEMGLD
                     KIFERCSEPKETNRQIGPMFHDWLKRKSLGIEPVPLDEFLSNDKDAILDAGDNAMMNF
                     AKEHLGYNHNKGL"
     gene            1349..2521
                     /locus_tag="PK345_07780"
     CDS             1349..2521
                     /locus_tag="PK345_07780"
                     /inference="COORDINATES: protein motif:HMM:NF027441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4831 family protein"
                     /protein_id="HPX34951.1"
                     /translation="MKKWMILIILLALFTSLETIGQVSVISVSKPSGKPSGTGMYYTL
                     PRTTLRVDVELKIIGRHRGPLSDYAEKFLGITNAIKFDDTLYELGNISVTPLSEPDPE
                     EIYYIELGLRDQRNPKKAIVEINEEGFLVFVNNLDTEPKTATEEKVVFLVDQAGYSSE
                     LNDNFLLTGRVNLSTDTIIRRVAVDTIMVEQLSFRKLALEKPGQDMAAEVVEKIEELR
                     SARMKLLTGFQEVAYDAEAIKYMDKQIREMEDDYLDFFRGKSLIRTKNYSFYYTPPAK
                     LDKSVIPLFRFSSADGIGALRGGSGDNIDLDFKISGSEVSIKNFSSSTSADPNLNGIA
                     YRIPESVIVSIKLGETELFSRKMLINQLGAVRRLPVQKVKAEFSIKTGGIKSLVIE"
     gene            2639..3547
                     /gene="xerD"
                     /locus_tag="PK345_07785"
     CDS             2639..3547
                     /gene="xerD"
                     /locus_tag="PK345_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009001080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009009 - site-specific recombinase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HPX34952.1"
                     /translation="MNWEVYIRGFKSFLRLEKSVSPNTIEAYIRDLERLMLFLDEAGY
                     NKLTPEKLTLSHFEEFIGWLGKKGYNASTQSRTISGIRTFYKYLMIEDIIENDPTELI
                     ERPRLVRQLPDTLSQKEIQNMIEAIDLSKPEGTRNLAIIEMLYGCGLRVSELTNLKLS
                     DLYFDLGFIRVLGKGKKQRWVPIGDHAVKQAMIYIENWRSLTLPKKGSEDIVFLNRRG
                     EKMSRVMIFYILKDLAQRAGIRKTISPHTLRHSFASHLVENGAGIRAVQDMLGHSSIT
                     TTEIYTHLNIKMLRETLDKYHPLYKK"
     gene            complement(3589..4971)
                     /locus_tag="PK345_07790"
     CDS             complement(3589..4971)
                     /locus_tag="PK345_07790"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPX34953.1"
                     /translation="MLAKHSMKDSRLSRWGILILVASIQAANYYFYDAISPLKRTLET
                     EFGFTSSDFGLFVSFYSIPNTFLLMAVFGGIILDRLGIRRTGILFIGLMALGGFITAY
                     GASTFYTSGGPFYEVMGSFWKRYSPELKMMLLGRFFFGLGAETSIVVISKIIVKWFIG
                     KELAMALGTKIAIARLGSFAAFNFSPYLKETASHWTIAIWFAALLLGIAFLGFLFYSW
                     FDLKIDRQLTDTATESKRSKFSFKDFKKLITTPSFIYITLLCVTFYSAVFPFQSFSAD
                     FFLNKFNLTQQQSGQIASYLSLGTLFFTPFFGFLVDRFGRSATLMIFGSLLLVLIHLT
                     FAFTNINPVIPMIFLGIAFSLVPAAMWPSVAKIVSQERIGTAFGAMYSIQNLGLWAFP
                     LLIGIVLDKTNPAITPEIIASGNYTYDYTWTLIMLAGLGILGLLFAILLKREDKQSGF
                     GLELSAKKKF"
     gene            complement(5127..>5627)
                     /locus_tag="PK345_07795"
     CDS             complement(5127..>5627)
                     /locus_tag="PK345_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004332025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HPX34954.1"
                     /translation="AMILTPFIGAFLDKKGLGASMMLYGAILLTVSHLIFALVPASAF
                     NHTIAYTTIVILGLAFSLVPASMWPSIPKIVEERFLGSAYGLTFWIQNIGLLLVPILI
                     GWSIVVANPGIKELIDAGDTTVKYNYMVPELIFAGFGVLAIIMSLVLKSVDKKKGFGL
                     DLPNKK"
BASE COUNT         1794 a         1046 c         1098 g         1689 t
ORIGIN      
        1 taatcctttg ttgtgattat agcctaaatg ttcctttgca aagttcatca ttgcattatc
       61 ccctgcatct aaaattgcat ctttgtcgtt gcttaaaaat tcatctaaag gtactggttc
      121 aatgcctaac gattttcttt ttaaccaatc atgaaacata gggccaattt gcctatttgt
      181 ttcttttggt tcgctacatc tttcaaatat tttatcaagc cccatttcat aaagccgtcc
      241 acagattcgg tttattgttc ttggatttct gtcaatagcc gaactgtctc gttttaaata
      301 tgcaatataa ctgtctttaa taggaaataa gtctaatttt aacaactgtc ttatcagtaa
      361 atcattatcc tttttcttga atgatttttc aacttctgcc caaatgtctt tatttatttc
      421 cctaattcct tccggaatag ttgggtaaac ttgaaataag tcatccaaat acgacctttg
      481 gtttgcatat tctatgctta atttagtcca tttattcata ttgcaaatat cgtcatttta
      541 ctttgttttg ttattttttt tagccttgcc gttaactcgt ttacatgtat cattttatgg
      601 agccaatctg cctttttggg ggtattggta tcataaagtt gcactaatct tacaaaggta
      661 aaaatatttg tcgttattgt ccggcaaaaa aattaaccgg atcagatttt gaatatccaa
      721 cccggaatct attcttttga attctgatac aaaaaaagta aaagatgggt agaagtacaa
      781 attttatcgg acagccgctt gagtgatgcc gatctgagtg gtctttatat aatggattcc
      841 gttaccatca ttaaatcgag tgagaaggtg cccttggtcc gatttgttac agcgatgtgg
      901 tgatataatc gaaacaggtg ttttggatga ggcgccgata ttttactcta tctccttgaa
      961 aaatctataa aaaatctttg cgctatcgct attacatcag cagcttgctg acatcaatag
     1021 gataaccaaa gagagtttaa aagggttaaa aaaatcattt aagacgatat tatcactatt
     1081 aacggtaaac attgcgagat tctcaatttt ttaatgctta aaatgatttt acgaaataac
     1141 taacctttgt ggattttatc ataatcgcct gatctttgct atatcccttt tattctattg
     1201 atattttaag aagtcaaaca gaggataatt tcaaattgtc tattttcgct ttaaataatt
     1261 cgccttaacc taatgaatta atatagtgaa aacgatgcga tcagttactt tcagttttct
     1321 tttttttgaa atttaaaaca gtatagatat gaaaaaatgg atgattctga ttattttgct
     1381 tgcccttttt acctctttgg aaacaatcgg gcaggttagt gttatttctg taagtaagcc
     1441 atcgggcaag ccttccggga caggaatgta ctatacatta ccacgcacca cattgcgagt
     1501 ggatgtggag ctaaaaatta ttggacggca tagaggacct ttgtcggatt atgcagaaaa
     1561 atttcttggt attactaatg caatcaaatt tgatgatacc ttgtacgagc ttggtaacat
     1621 ttcagttaca ccattgtcgg aacccgatcc tgaggagatc tattacatcg aattaggctt
     1681 gcgcgaccag cgtaatccga aaaaagcgat tgttgaaata aatgaagagg gctttcttgt
     1741 ttttgtaaat aacctcgata cagagcctaa aacggctacc gaggagaagg ttgtcttttt
     1801 agttgaccaa gcaggatatt catcagaact gaatgataat ttcctcttaa ccggaagggt
     1861 taacctctct accgatacca ttatcaggcg tgtggctgtt gacactatca tggttgagca
     1921 attgagtttt cggaaactgg cgttagaaaa acccggtcag gacatggcag cggaagttgt
     1981 tgagaagatt gaggagctta ggtcggcacg catgaagttg cttacagggt ttcaagaggt
     2041 agcctacgat gctgaagcaa taaaatatat ggataaacaa atcagggaaa tggaagatga
     2101 ctatctcgat ttttttagag gtaaatcctt aatccgtacc aaaaactact ctttctatta
     2161 cacccctccc gcaaagcttg ataagtctgt gattccgctt tttaggttct cctctgctga
     2221 tggcatcggc gcattaaggg gtgggtctgg cgataatatt gatcttgatt ttaaaatcag
     2281 cggttctgag gtgtcaataa agaatttttc atcatctact agcgccgatc ctaacctcaa
     2341 tggaattgct taccgtattc ctgaaagcgt tatcgttagt ataaagcttg gcgaaacaga
     2401 gctttttagc cgtaagatgt taataaatca gttaggggct gttcgtcggt tgccggtaca
     2461 aaaagttaaa gctgaatttt ccataaaaac cggtggcatc aaaagccttg tgatagaatg
     2521 atcttgatcc cactctatag ataaaacgcc ttgttttcaa taagaaaaca gacaaacaaa
     2581 agttattagc taataaaatg attttgtaaa ataagaatta tttcaagaaa aaagggtgat
     2641 gaattgggag gtgtacatcc gcggatttaa atcttttctt cggctcgaaa aatcggtttc
     2701 gcccaacaca attgaagctt atatccgtga tttggagcgg cttatgttgt ttcttgacga
     2761 agccggttat aataagctaa ctcccgaaaa gctaaccctg agccacttcg aggagtttat
     2821 tggctggctt ggcaaaaaag gctataacgc ttccacccaa agcaggacaa tttcagggat
     2881 acgtactttt tataagtacc tgatgataga agatatcatt gaaaacgacc ctaccgagtt
     2941 gattgagagg cctcgtcttg tcagacaact tcccgatact ttaagccaga aagagataca
     3001 aaacatgatt gaggccattg atctttcaaa gcctgaaggt actcgtaatc tggctatcat
     3061 tgagatgcta tacgggtgtg ggcttcgggt ttcggaactg actaatctga agctgtccga
     3121 tctctatttt gatcttggat ttattcgggt tttaggtaaa ggaaaaaaac agcgttgggt
     3181 tccgattggc gatcatgcgg taaagcaagc tatgatttat attgagaatt ggcgctcgct
     3241 gacattgcct aaaaagggta gtgaagatat tgttttcctt aatcggcgtg gcgaaaagat
     3301 gtcgagagtg atgatatttt atattttaaa agatttggca caacgagccg gtatccgcaa
     3361 aaccatcagt ccacatactt tacgacactc atttgcatcc catcttgtgg aaaatggcgc
     3421 cggcattagg gctgttcaag atatgcttgg tcactcatca ataaccacta ccgaaattta
     3481 tacacatctc aacattaaaa tgcttcgcga gacactggac aaatatcatc cattgtacaa
     3541 aaaatgatta taatgtaatg gtcaaggtta cccttgcctg aaaaattact aaaatttctt
     3601 tttagccgat aattcaagtc caaatcccga ttgcttgtct tcgcgtttga gcaggatagc
     3661 aaacagtaac cctaaaatac caagacctgc aagcatgata agagtccatg tgtagtcgta
     3721 agtatagttg ccgcttgcaa ttatctcggg ggttattgca ggattagttt tatcgagaac
     3781 aataccgatt aataaaggaa atgcccacaa acccaagttt tgaatcgaat acattgcgcc
     3841 aaatgcagta ccgatacgtt cctgtgaaac gatttttgca acggaaggcc acatggcggc
     3901 aggtacaaga gaaaaagcaa tacctaaaaa aatcataggt attaccggat ttatattagt
     3961 gaaagcaaaa gtgaggtgaa ttaatacaag cagtagagag ccgaaaatca ttaacgtggc
     4021 gctacgacca aaacgatcta ccaaaaagcc aaagaagggt gtgaaaaaga gtgttcccag
     4081 actgagatat gatgcaattt gaccgctttg ctgttgggtc aggttaaact tattaagaaa
     4141 gaaatcagcc gaaaacgact gaaacgggaa tacggcagaa taaaaggtaa cacacaaaag
     4201 ggttatataa ataaatgaag gagttgttat caatttttta aaatccttaa agctgaactt
     4261 tgatctttta ctttcagtag cagtatcggt caattgccgg tctattttta aatcaaacca
     4321 tgagtaaaaa agaaatccca aaaatgctat tccaagaagc agggcagcaa accatattgc
     4381 tattgtccag tgcgatgctg tttctttcaa atagggcgag aagttaaagg ctgcaaacga
     4441 tccaagcctt gcaatagcaa tttttgtccc caaagccatt gccagctcct ttcctataaa
     4501 ccacttaacg attattttac tgatgaccac aatacttgtc tcggcgccta atccaaaaaa
     4561 gaatcgtcct aaaagcatca tttttaactc tggcgaatac ctcttccaaa aggatcccat
     4621 aacttcgtaa aaggggccac ccgatgtgta aaaagtgctg gcgccgtaag ccgtaataaa
     4681 gcctccaagc gccattaaac caatgaagag tatgcctgtg cgacgtatgc ctaaacggtc
     4741 gaggatgatg ccgccaaaaa ccgccatcag caggaaagtg ttagggatgg agtagaatga
     4801 gacaaaaaga ccaaaatcag aggaggtaaa cccaaactca gtttcaaggg ttcgtttcag
     4861 tggcgatata gcatcgtaga aatagtaatt tgcagcctga atggaggcta ccagaataag
     4921 tatgccccag cgacttaacc gcgaatcctt catactatgt tttgccaaca tcagtaaatg
     4981 tgatatttag aaattaaatg gaattaaaag gatgttaatc caaatatgaa taatccttac
     5041 ccttgctttt gaaaaacaaa gggtttgaat attaaaattt gcgttgctaa tagatcgtta
     5101 gatacttttt atctgctcca aaaggattat tttttgtttg gcaagtcaag tccaaatcct
     5161 tttttcttat caaccgattt cagtaccaac gacataatta ttgccaatac tccaaatccg
     5221 gcaaaaatca actcgggcac catataatta tattttacag ttgtatcgcc ggcatcaata
     5281 agttccttaa ttccaggatt tgccaccaca attgaccatc caataagaat tggaactaaa
     5341 agtaacccaa tattttgaat ccagaaggtc aatccatacg cagaaccaag aaaacgttct
     5401 tctacaattt taggtattga tggccacatg gaagcgggaa ctaatgagaa agccaagccg
     5461 agtattacaa tagtagtata ggcaatagtg tggttgaatg cacttgcagg tactaaagca
     5521 aaaatgagat gtgaaacggt aagtaaaatt gcaccataaa gcatcattga agcgcctaat
     5581 ccttttttat caaggaacgc tccgataaag ggcgttaaaa tcatagc
//
LOCUS       DAOOAI010000117         5295 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_17840_length_5295_cov_4.252863, whole genome shotgun sequence.
ACCESSION   DAOOAI010000117 DAOOAI010000000
VERSION     DAOOAI010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5295)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17840_length_5295_cov_4.252863"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..2074
                     /locus_tag="PK345_07800"
     CDS             <1..2074
                     /locus_tag="PK345_07800"
                     /inference="COORDINATES: protein motif:HMM:NF019325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34955.1"
                     /translation="RIALTQDNTLIGRAFADDNGNANIGLSEIISSSSQITISITAHN
                     KELHLGTINVIADIPYVVADYFTINDDIGNANGQADYGEKILLGLGMKNLGNLPANDV
                     TVKISSFDSYITLSYLAVEFGDIGPGETVFIDDAFPVIISEKIPDQHEVQIYVKAESD
                     DTWISNIYFTVNAPSIKTINMTIDDEEGGNGNSKLDPGENVIFRFDVKNNGHALSPDI
                     SIQLTSNNSKVVITSEPINQQGLEPDEEYQVEFTAIVDTEAVMGDVASLQAKIIAGEY
                     ITTDNYLFKIGMITEDFESSDFSLLNWEFDGDKPWFISETDPWEGLFCAQSGDITHEQ
                     KSELKISVFVAQIDTVSFYQKVSSETNKDFLRFYDGPLLKKEWSGNNDWEEAFFIVYP
                     GYHEFRWVYEKNNENSHYDDCARIDYIKFPKLLESKLEAGGDDMVCIGDDHQVKSSGI
                     YIMSIQWETSGSGTFNNPNTLQPIYTPSEEDILNGEVILSVSAIGANNQPLSDSMTLE
                     IIDEPPIPLKPVGEIQVCTNFGKSYQYSTQEFPEIRAYIWELNPEEAGSWLGNENIID
                     IVWTPDFVGNISLKVKGVNPCGESIFSDSLNIEAFICDAIEEKTKEELVIYPNPTDRI
                     IYLTIPGEMSENEIVIYNSTGIEVFRQKDENIVTELKLDLHNLPKGIYLLKTVNDRKV
                     LTAKLILK"
     gene            2320..3456
                     /locus_tag="PK345_07805"
     CDS             2320..3456
                     /locus_tag="PK345_07805"
                     /inference="COORDINATES: protein motif:HMM:NF020965.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HPX34956.1"
                     /translation="MKTFGIKKKLPKIMLLSIIAVTSFFYKELICQNIKQPFLSTIHI
                     ETKIHQGLVINHHPEMWALTDGYFPSYEIGLARQTVGKNSEVYFRKYPRYGLTYRYTN
                     FNGSPYLGEAHSAMAFVSLKVAKCNIVELNFRTSLGIAYLTKTFDYKYNYKNRAISTN
                     WNASIGFELSSAWKLSKIAELSTGITMLHLSNGTIKTPNYGLNIPALEAGLSFKLNRA
                     DVRYKIPETLILRKGKNNLRASFGMAYKEIIERWQEKFQVFTGDIVFTHFYNNVNRYI
                     IGFDGAYDQSNKIIIESQGDTTDTWVDESKFGAIVGHEWVISRFAINFSVGTYIYNLN
                     KSNALVYNKIGVIYFFHNNIFGGITLKSHYAKADFLSANLGISL"
     gene            3517..4215
                     /locus_tag="PK345_07810"
     CDS             3517..4215
                     /locus_tag="PK345_07810"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2807 domain-containing protein"
                     /protein_id="HPX34957.1"
                     /translation="MLSCKNDLFNECLSGRGKVTTERRGLYPFGNIAVYDNINLTVVQ
                     ANEYSITLYGGDRLLPLITNLIINNTLELRNQSRCNLLKDPWEPVEVIVKTPKLDSLF
                     IRSQGKISNSIPIKQDNLYVSAYETSADIHLDLATRFFRFDNKKGTADMYLYGHSDTV
                     FIYNIGAGRVNATTITSDVMVVNTGSMNDTWVKSGKKILDVKITYMGNVFYSDNPEEI
                     IYYSAGSGKLLLIN"
     gene            complement(4212..>5295)
                     /locus_tag="PK345_07815"
     CDS             complement(4212..>5295)
                     /locus_tag="PK345_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HPX34958.1"
                     /translation="TYRNWIQSYRDLPLLINQWANVVRWEMRTRLFLRTAEFLWQEGH
                     TAHSTKEEAIEETLKILDIYADFAEKWMAVPVLKGLKSANERFAGAVDTYCIEALMQD
                     GKALQAGTSHFLGQNFAKAFDVKFLSKDNQLEYVWATSWGVSTRLMGALIMAHSDDNG
                     LVLPPKLAPIQVVIVPIYKNYDQLNEISIKANEIKNSLIAKGISVKYDDRDTQKPGWK
                     FSEYEFKGVPVRLAIGPRDLENGTVEVARRDTLEKEIYQITDIENKVIHLLDQIQVNL
                     YHKAYAFREENTYRVDTWDDFKVQIEKGGFILAHWDGTSETELKIKDETKATIRTIPL
                     DNKQEVGFCVYSGRPSKERVVFAKAY"
BASE COUNT         1741 a         1073 c          985 g         1496 t
ORIGIN      
        1 cagaattgct ctaacgcaag ataatacgct aattggccgt gcatttgctg atgataatgg
       61 caatgccaac atcgggcttt cagaaataat atcctcaagt agtcaaataa ccatctcaat
      121 cactgcacac aacaaagaat tacacctcgg aactataaat gtgattgcgg atatacctta
      181 cgtagtagcc gactacttca ctataaacga tgatattggt aatgccaacg ggcaagccga
      241 ctacggagag aaaatcctat tggggttagg catgaaaaac ctggggaatc ttccggcaaa
      301 tgatgttact gttaaaatat cctcctttga tagctatatt acactctcat atttggctgt
      361 agaatttgga gatattgggc cgggcgaaac ggtatttatt gacgatgctt tccctgtaat
      421 catctccgaa aaaattcccg atcagcacga agtacaaatc tatgttaaag cagaaagcga
      481 cgatacatgg atatctaaca tttattttac agttaacgct ccgtcaatta aaaccataaa
      541 tatgacgatt gatgacgaag aaggcggcaa tggcaattca aagcttgacc ccggagagaa
      601 tgtaattttc agatttgacg taaaaaacaa cggacatgcg ctatcacccg atatttcgat
      661 acaactaacc tccaacaatt caaaggttgt aattacctcc gagccaataa accagcaagg
      721 gttagaacct gatgaagaat atcaggtcga gtttacagcc atagtagata cagaagctgt
      781 gatgggggat gtcgccagcc tgcaagcaaa aataattgct ggagaatata tcacaacaga
      841 taactactta tttaaaattg gaatgataac cgaagatttt gaatcttccg acttctcgtt
      901 gttaaattgg gagttcgacg gcgacaagcc ttggtttatc agtgaaacag acccatggga
      961 agggcttttc tgcgctcaat cgggagatat aacccacgaa caaaaaagcg aattaaaaat
     1021 aagtgttttc gtcgctcaaa ttgacactgt ctctttttat caaaaagtat caagcgaaac
     1081 taataaagac ttcctccgtt tttacgacgg accactatta aaaaaagagt ggtcaggaaa
     1141 taacgattgg gaagaagcat tttttattgt ctatccgggt tatcatgaat tccgttgggt
     1201 ttatgaaaaa aataatgaga atagccacta tgatgactgt gcacggattg attacatcaa
     1261 atttccaaaa ttacttgaaa gtaaacttga agcgggtggt gatgatatgg tgtgtatcgg
     1321 cgatgatcac caagtaaaat cgagtggtat ctatattatg tcaatacaat gggaaaccag
     1381 cggttcaggc acatttaaca acccgaacac tttacaacct atttatacac caagcgaaga
     1441 agatatttta aacggcgaag taatactatc agtatcagcc attggagcta ataaccaacc
     1501 gctctccgac agtatgactc tcgaaataat tgacgagcct cctatacctt taaaacccgt
     1561 tggagagata caagtgtgca ccaattttgg aaagagttat caatactcta ctcaggaatt
     1621 tcccgaaata agggcttaca tttgggaact taaccccgaa gaggcaggct cgtggctcgg
     1681 gaacgaaaac attatagata ttgtgtggac gcccgacttc gtcggtaaca tcagcttaaa
     1741 agtaaaaggt gtaaacccat gcggcgaaag catcttctca gatagcctca atatagaagc
     1801 cttcatctgc gacgccattg aagagaaaac aaaagaggaa cttgtaattt atcccaatcc
     1861 gaccgatcga attatttatt taactatacc aggagagatg tccgaaaatg aaatagtgat
     1921 ttataactca accggcattg aagttttcag acagaaggat gaaaacatag taaccgaact
     1981 gaaattagac ttgcataatc tgccaaaagg catatacctt ttaaaaaccg taaacgacag
     2041 aaaagtatta acggctaagc taattcttaa atgatcgaac atttaaaata aaatccaagt
     2101 ttgcaaacta cgccttgata tattagtatc atctatttcg atattaaata aagtcaaaaa
     2161 gctgttgaag cggctataaa aagattatta aagaaacagg gcgttcggcc cgatatgcaa
     2221 atgccgacaa cgaaaactgt tttaaaaaca ggcagaaatg ataacatagg aaattacaat
     2281 gtaaaaaaca atacagcata aaacgagtag gctggcataa tgaaaacctt cggcataaaa
     2341 aagaaactcc ctaaaattat gcttttgagc ataatagcag ttactagttt tttttacaaa
     2401 gagcttattt gtcaaaatat aaaacagcct tttctttcga ccatacatat cgaaactaag
     2461 attcatcagg gtttagtcat caatcatcat cctgaaatgt gggcgcttac tgatggctat
     2521 ttcccttctt acgaaatagg attagcgcgt caaaccgttg gtaaaaactc agaggtctat
     2581 ttcagaaaat atcctcgtta cggcttgaca taccgttaca caaattttaa cggctctcca
     2641 tatcttggcg aggcccattc agccatggca tttgtcagtt tgaaggttgc aaaatgcaat
     2701 atcgttgaat taaatttcag aacatcactt ggcatagcgt accttacaaa aacttttgac
     2761 tataaataca attacaaaaa cagggcaatc agtaccaact ggaatgcatc tattggattt
     2821 gaactaagct cagcatggaa attatcgaag attgccgaac tttccacagg tatcacgatg
     2881 cttcatctct ccaacggcac cataaaaact ccaaactatg gtttaaacat accggctttg
     2941 gaagcaggct taagctttaa attaaataga gccgatgttc gttacaagat cccggaaacg
     3001 ttaatcctgc gaaaagggaa aaataattta cgagcatctt tcgggatggc ctataaagag
     3061 attattgaac gctggcagga gaagttccag gtgtttaccg gcgatattgt cttcactcac
     3121 ttttacaata atgtaaatcg ctatatcatt ggctttgacg gcgcttatga tcaatcgaac
     3181 aaaataataa tcgaatcgca gggagatacg accgatacat gggttgatga atcaaaattt
     3241 ggagctattg ttgggcatga atgggttatt tcaaggtttg caataaactt ttcggtagga
     3301 acatacattt acaacttaaa caaatcaaac gcccttgttt acaataaaat aggtgttatt
     3361 tatttttttc ataacaatat tttcggcggt attacattaa aatcgcatta tgcgaaagct
     3421 gattttttat ctgctaattt gggaataagc ctttaaatga tgcaacgcct cttttttaca
     3481 attttaaaat attattgtct tttacttcta tttaccttgt tatcttgcaa gaacgatctg
     3541 tttaacgagt gtctttcggg cagaggtaaa gttacaacag agcgtcgagg attgtatccc
     3601 tttggtaata ttgccgttta cgataatatt aaccttaccg tcgtacaggc aaacgaatat
     3661 tccatcaccc tttacggtgg agatagactc ctccctttaa tcacaaactt aatcattaac
     3721 aatacgcttg aattgagaaa ccaaagccgc tgcaatttgc tgaaagaccc gtgggaacca
     3781 gtggaagtta ttgtaaagac tccaaaactt gattcactat ttatcagatc gcaaggaaaa
     3841 atttcaaact caattcctat caaacaagat aatctatacg taagcgccta cgagacttcc
     3901 gccgatatcc acctcgatct ggccacccgt tttttcaggt tcgacaataa aaaaggaact
     3961 gctgacatgt acctctatgg acatagcgat acggttttta tttacaatat tggcgctggt
     4021 agagtaaacg ctactacaat aacatcagat gtgatggttg ttaacaccgg cagcatgaac
     4081 gatacctggg taaaatccgg caaaaagatc cttgatgtaa agataaccta tatgggaaat
     4141 gttttttact cggacaatcc tgaagaaatt atttattact ccgccggtag tggaaaactg
     4201 ttgttaatca attagtaagc tttagcaaat accactctct ctttacttgg ccgtccgcta
     4261 tagacacaga aaccaacttc ctgtttatta tccaatggta ttgtgcgtat ggtggctttc
     4321 gtttcatctt tgatttttaa ttcggtctcg ctagtgccat cccaatgagc caaaataaaa
     4381 ccaccctttt ctatctgaac tttaaagtcg tcccatgtat caacacgata agtattttct
     4441 tcacggaacg cataagcttt atgataaaga ttcacctgta tttgatcgag tagatgtatg
     4501 actttatttt caatatctgt aatttgatat atctcttttt caagtgtgtc gcgccttgcc
     4561 acctctacag ttccattttc aagatctctg gggccaattg cgagcctcac aggaacgccc
     4621 ttgaactcat attcggaaaa tttccatccg ggcttctgtg tatcacggtc atcatatttc
     4681 accgatattc cctttgcaat caacgaattt tttatctcat tggcttttat gctgatctca
     4741 tttagctgat catagttttt ataaatgggc acaataacaa cctgtattgg cgcaagcttt
     4801 gggggaagca caagtccatt atcatcagaa tgtgccataa taagcgctcc catcaatcgg
     4861 gtggatacgc cccacgaggt agcccaaaca tactccaact gattatcttt ggaaagaaac
     4921 ttaacatcaa aggcctttgc aaaattctga cctaaaaaat gcgatgtacc tgcctgcaat
     4981 gcttttccat cctgcataag cgcctcaata caataagtgt caactgcgcc ggcaaaccgc
     5041 tcatttgccg acttcaagcc ctttaaaacc ggaacagcca tccatttctc agcaaaatca
     5101 gcatatatat ctaaaatttt taaagtctcc tctatggctt cttctttggt agagtgggca
     5161 gtatgaccct cttgccataa aaattcagca gtgcgaagga aaagacgcgt ccgcatctcc
     5221 cacctcacca cgttagccca ctggttgata aggagaggca aatcacgata ggactgtatc
     5281 caattacgat atgta
//
LOCUS       DAOOAI010000118         5195 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_18273_length_5195_cov_4.304864, whole genome shotgun sequence.
ACCESSION   DAOOAI010000118 DAOOAI010000000
VERSION     DAOOAI010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5195)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5195
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18273_length_5195_cov_4.304864"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="PK345_07820"
     CDS             <1..476
                     /locus_tag="PK345_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR01947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit G"
                     /protein_id="HPX34959.1"
                     /translation="KDPIAEVDRQKQQKAIEMVMPGFTELNRFAILPHSGGDSLIFYE
                     GIKDGEVIGVAINTYTHLGYNGLIRIMVGFTPDGTIVSYQVLEHKETPGLGSKIADNF
                     KEQFDGKNPAAFSLKVRKDGGDVDAITAATISSRAVCDALSRAFQAYEEVKQKQN"
     gene            491..1075
                     /locus_tag="PK345_07825"
     CDS             491..1075
                     /locus_tag="PK345_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019131087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit E"
                     /protein_id="HPX34960.1"
                     /translation="MNQLQNLTKGFFKENPVFVMLLGMCPTLATTTSANNGLGMGLAT
                     TFVLVMANIAISLIKNLVPDKVRIPIYIVVVAAFVTVVDLVMAAYAPALHSQLGIFIP
                     LIVVNCIVLGRAEAFASRNNIFSSMIDGLGMGLGFAMALTILGSVRELIGNFSIFGYK
                     FIQSDGILIFILSPGAFIALGFLIALINLITKKA"
     gene            1094..1666
                     /gene="rsxA"
                     /locus_tag="PK345_07830"
     CDS             1094..1666
                     /gene="rsxA"
                     /locus_tag="PK345_07830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009017737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HPX34961.1"
                     /translation="MEYIIMIISTVLVSNIVLANFLGICPFLGVSGKLSTASGMAGAV
                     LFVMTVSSIVTWLVQQYILTPLGLSFLQTIAFILIIAFLVQVVEIILKKVSPPLYQAL
                     GVFLPLITTNCTILGVALLITQKQYNLIEGTVYAIGNALGFALALFIFAGIRENLELM
                     EVPKGLRGMPIALITAGILSLAFMGFANLV"
     gene            complement(1774..2964)
                     /locus_tag="PK345_07835"
     CDS             complement(1774..2964)
                     /locus_tag="PK345_07835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HPX34962.1"
                     /translation="MLTNRQIFFQHLALPSQMPLGLEVERAEGIYMYSPEGKRYIDLV
                     SGVCVNNLGHRHPAIVRAVKDQLDNYMHLMVYGEMIQTPQVKLAQRLTDLLPRNLNSV
                     YLVNSGSEAIEGALKLAKRFTGRTEIIGFKNSYHGGTHGALTMLGNEEMKYAYRPLLP
                     DVRFLQFNSKEDLFQITERTACVLVEPVQAEAGVIMPKDDFLMTLRQRCSETGTLLIF
                     DEIQTGFGRTGKLFCFMDYNVTPDILCIAKAMGGGMPIGAFIADKRIMLTLTHNPDFG
                     HITTFGGHPVSAAAAIATLEELTKNPNLINQVDEKGAIYESALKNHSLVKGIRRKGLL
                     ISIELPTVEINQKIIQELLQNGLVTDPFFFMPQAFRIAPPLIITKDEIALTLELINKT
                     FKNL"
     gene            complement(3030..3416)
                     /locus_tag="PK345_07840"
     CDS             complement(3030..3416)
                     /locus_tag="PK345_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009133847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein"
                     /protein_id="HPX34963.1"
                     /translation="MEKPLRIDKWLWTVRVFKTRSIATDACRSGKVEINNQQVKPSRE
                     IKLNDVIKVTINTHFTRTLIITGFPISRVAAKLVAQYATEITPAEEFEKLKKFNELNW
                     EKRERGLGRPTKKERRLIDKLKNDIP"
     gene            complement(3421..4725)
                     /gene="der"
                     /locus_tag="PK345_07845"
     CDS             complement(3421..4725)
                     /gene="der"
                     /locus_tag="PK345_07845"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013618627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HPX34964.1"
                     /translation="MSNILAIVGRPNVGKSTLFNRLIGKRQAIVEESSGVTRDRHYGK
                     SDWNGISFSVIDTGGYVLGSEDIFEEEIRKQVDLAISESDVIVFVLDTRDGVTEPDKD
                     VAQILRKSEKPVLLVANKVDSTLQIHDINEFYELGFEKIYPISAINGSGTGDLLDEVV
                     SYFKESEEETETDIPRLAIVGRPIVGKSSFINTLIGVERNIVTDIPGTTRDTIHTRYN
                     SFGFDFILVDTAGLRKKSKVSENIEFYSVMRSIRAIENSDVCMLMVDATQGFESQDLN
                     ILHLIEKNRKSLVIVVNKWDLISKQTNTHIEFENLIRNLTAPFTDYHIIFTSVIKKQR
                     IFKAMEAASEAFKNKTKRIATSKLNDILLPIIKASPPPIYKGKTISIKYITQLPVAFP
                     AFVFYCNLPQYIREPYKRFVENNLRKHFGFTGNPVQIYFRKK"
     gene            complement(4850..>5195)
                     /locus_tag="PK345_07850"
     CDS             complement(4850..>5195)
                     /locus_tag="PK345_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007365512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HPX34965.1"
                     /translation="LTDRTYRFFVSEIIRGKILNLYQKEVPYSVEVVVDEFKEEPEIT
                     RIRALIFVARESQRIILIGHKGNMIKKLGIEARKEIESFLQQKVYLELTVKVDKDWRD
                     SEQALKRFGYII"
BASE COUNT         1478 a         1063 c          963 g         1691 t
ORIGIN      
        1 ccaaagaccc aattgccgaa gttgatcgtc agaaacagca aaaggccatc gaaatggtaa
       61 tgcctggatt tactgagtta aatcgctttg ccatattacc gcactcgggc ggtgattccc
      121 tgatttttta tgaaggaata aaagatggtg aagttatcgg tgttgcaata aacacctaca
      181 ctcatctggg gtataatggc ctcatacgga taatggtagg ttttacaccc gatggtacta
      241 ttgtaagtta tcaggtgctt gagcataagg agacaccagg attaggcagc aaaatagcag
      301 ataatttcaa agagcagttt gacggtaaaa atcctgcagc gttttcttta aaagttagaa
      361 aagatggggg agatgtagat gctatcacag cagccacaat cagctccaga gctgtttgcg
      421 atgctttgag cagagctttt caagcgtatg aagaagtgaa acaaaaacaa aattaacagg
      481 agatagggtt atgaatcaat tacaaaactt aactaaaggc ttttttaaag aaaatcctgt
      541 atttgtgatg ttgcttggca tgtgcccgac tctggcaaca actacttcgg ccaataatgg
      601 cttgggcatg ggcttagcta ctacctttgt gctggtaatg gcaaatattg ccatctcgct
      661 cattaaaaat ttagtgcccg ataaagtgag aataccgatc tatattgttg tcgttgcagc
      721 ttttgttact gtggttgacc ttgttatggc cgcctatgct ccagcgctgc attcacaact
      781 tggaattttt atccctctta tcgttgtcaa ttgcatcgta ttaggccgtg cagaggcttt
      841 tgcatcacgg aacaacattt tctcttcaat gattgacgga cttggtatgg ggcttggttt
      901 tgcaatggct cttactatct taggctcggt gagagagctg ataggaaact tttcgatttt
      961 cggatataag tttattcaat ctgacggcat tctgattttt attttatctc ccggagcttt
     1021 cattgcgttg ggatttctta ttgcacttat taatctaatt accaaaaaag cttaatctct
     1081 aacacttttc actatggaat acattataat gataataagt acagtgctgg tcagcaatat
     1141 tgtgctggcc aactttctcg gaatctgtcc ctttcttggt gtttccggaa aacttagcac
     1201 agcatcgggt atggccggtg cagtactttt cgtgatgacc gtttcgtcta tcgttacatg
     1261 gcttgttcag caatatatac ttacaccgct tggtcttagc tttttacaaa ctattgcttt
     1321 cattctgata atcgcttttt tagtacaggt ggttgagatt attctaaaaa aggttagccc
     1381 gccgctctat caggcgcttg gcgttttctt acctcttatt acaactaact gcacaatatt
     1441 gggagtggcg ctactcatca cacaaaaaca atataacctt atcgaaggta cagtgtatgc
     1501 aataggtaat gcattaggtt ttgcacttgc gctcttcatc tttgcaggta tccgcgaaaa
     1561 cctcgaactg atggaggttc ccaaagggct gcgaggaatg ccaatagccc ttatcacagc
     1621 cggaatttta tcgcttgctt ttatgggctt tgcaaatctg gtttaaaccg aattattcat
     1681 cggaaagtca ttttgaactg aacgattgaa tcttgagcta ttcactggat gaaataaagt
     1741 cgtgtggtaa gcgcctttaa gtccccctgt gagttacaga tttttaaagg ttttatttat
     1801 caactcaagg gttagcgcta tttcatcttt tgtaattatt aatggcggtg caatacgaaa
     1861 agcctgaggc ataaagaaaa acggatcggt aactaatccg ttttgtaaaa gttcctgtat
     1921 tattttttga ttgatttcta ccgttggaag ttcaatactt atcagcagac cttttcggcg
     1981 gataccctta actaatgagt ggtttttaag cgctgactca tatattgctc ctttttcatc
     2041 tacctgatta atgagatttg ggtttttcgt taattcttct aatgtagcta ttgctgcagc
     2101 agcagatacg ggatgaccac caaaagtggt aatatgtccg aaatcaggat tatgagttag
     2161 cgtaagcata atccttttat cagcaatgaa agcgcctatt ggcattccgc cacccatagc
     2221 cttggcaata cataagatgt cgggtgttac attataatcc ataaaacaaa atagcttgcc
     2281 tgttcggcca aaaccggtct gtatttcgtc aaatatcaga agcgttcccg tttcgctaca
     2341 ccgctgcctc aaagtcatta aaaagtcatc tttaggcata attacaccag cttcagcctg
     2401 taccggttca accaaaacac atgcagttcg ttcggtgatc tgaaataggt cctccttgct
     2461 gttaaattgt aaaaatcgta catccggcaa caatggacgg tatgcatatt tcatctcttc
     2521 gttaccaagc attgtaagtg cgccatgtgt gccgccatga tagctatttt taaaaccaat
     2581 aatctctgta cggcctgtaa aacgcttggc aagcttcaag gctccctcaa ttgcctcgct
     2641 ccccgaattg acaagatata ccgaatttag gttacgaggt aaaaggtcag ttaacctttg
     2701 tgctaatttt acttgtggtg tctgtatcat ttcgccataa accatcaagt gcatataatt
     2761 atccaactga tcttttactg cgcgcactat tgccggatgc cgatgcccaa gattattcac
     2821 acacactccc gaaactaaat caatatatct tttgccttcg ggcgaataca tataaatacc
     2881 ttcggcacgc tcgacctcca aacctaaagg catttgtgac ggcaaggcaa gatgctgaaa
     2941 aaatatctgt ctgttagtaa gcattttaag cctgttttat tgaaataatt acttaaaacc
     3001 aattttttaa aatacaataa cgctgctggc taaggtatat catttttcaa tttatcaatg
     3061 agccttcgtt cctttttagt tggtcgccca agcccccttt cccgcttttc ccagtttagc
     3121 tcattaaatt ttttaagttt ttcaaattct tcagcaggag taatctctgt agcatattgc
     3181 gctacaagtt ttgctgctac acggctaatc ggaaagcctg taataataag cgttctggta
     3241 aaatgagtgt ttatcgttac tttgatcaca tcattaagtt ttatctctct tgatggctta
     3301 acctgctgat tgtttatttc tactttaccc gaacggcaag catcggtagc tatactcctt
     3361 gttttgaata ctcgtactgt ccagagccat ttatcaattc tcaaaggttt ttccataacc
     3421 ctattttttt ctgaaataaa tctgaaccgg gtttcctgta aaaccgaaat gctttcgcaa
     3481 attattttca acaaatcgtt tgtagggctc gcgaatgtat tgcggtaagt tacaatagaa
     3541 tacaaaagcc ggaaaagcaa cgggcagttg agtaatgtat ttgatactta tagtttttcc
     3601 tttgtaaata ggtggggggg aggctttgat gataggtagt aaaatatcat tgagctttga
     3661 ggtggcaata cgttttgttt tattcttaaa agcctcgctt gcagcttcca tcgctttaaa
     3721 gatgcgttgc tttttaatta ccgaagtaaa aattatatga taatcggtaa aaggagcggt
     3781 aaggtttcgg ataagattct caaattctat atgggtgttg gtttgtttgg atattaaatc
     3841 ccacttattg acaacaataa caagggattt ccgatttttc tcgatcagat gcaaaatgtt
     3901 taaatcctga ctttcgaaac cttgtgtggc atcaaccata agcatacaaa catcactgtt
     3961 ttcgattgcc cggattgatc gcatcaccga ataaaattca atattctccg atactttact
     4021 cttctttctc aatcctgcag tgtcaaccaa aataaagtca aatccaaaac tattgtatcg
     4081 tgtatgaata gtatcccttg ttgtgcctgg aatatctgta acaatgtttc gttcaacgcc
     4141 aataagtgtg tttataaatg aagatttacc aacaataggc ctgcctacaa tagctaatcg
     4201 ggggatgtca gtttcggtct cctcttcact ttctttgaaa tagcttacaa cttcatcaag
     4261 taaatccccg gtgccactac cgtttatagc cgatatcgga taaatttttt caaatcccaa
     4321 ttcataaaac tcatttatat catgtatttg gagtgtacta tcaaccttat tggcgaccaa
     4381 taatacaggc ttctctgatt tgcgtaaaat ttgtgcaaca tctttatcag gctcagtaac
     4441 tccatcgcgt gtatcaagaa caaacacaat aacatccgat tctgaaattg caaggtcaac
     4501 ttgttttctg atctcttctt caaagatatc ttcagatcct aaaacatagc ctcctgtgtc
     4561 aattaccgaa aagctaatcc cgttccagtc gctttttcca tagtggcggt cgcgtgtaac
     4621 accgctcgat tcctccacaa tagcctgtcg cttgccaatc aatcggttga aaagcgttga
     4681 ttttccaacg ttggggcgcc caacaatcgc caatatattc gacataactt tttttttatt
     4741 gattaataaa cttcaatttc tggccaataa caatattaac tttttaagat gaattgctgc
     4801 aaaataatca ttttaacctt aatatcaaga taattaaaaa gatagataat caaataatat
     4861 atccaaatcg ctttaaagct tgttcgctat cgcgccaatc tttatctact tttaccgtca
     4921 gctcaagata tactttctgt tgcaaaaatg attctatttc cttccttgct tctatgccaa
     4981 gcttttttat catgtttccc ttatggccga ttaaaataat ccgttggctt tcgcgagcaa
     5041 caaaaatcaa tgccctgata cgtgttattt ccggctcttc tttaaactca tccacaacga
     5101 cctcaacaga ataagggacc tccttttggt aaagattaag gatttttcct cgaataatct
     5161 ctgatacaaa aaagcgataa gtcctgtcag tcaat
//
LOCUS       DAOOAI010000119         5159 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_18401_length_5159_cov_5.794083, whole genome shotgun sequence.
ACCESSION   DAOOAI010000119 DAOOAI010000000
VERSION     DAOOAI010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5159
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18401_length_5159_cov_5.794083"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(89..1075)
                     /locus_tag="PK345_07855"
     CDS             complement(89..1075)
                     /locus_tag="PK345_07855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34966.1"
                     /translation="MIRKITIPFILTFVFSLFYSCETEVNLTTDWKEITIVYGLLSQM
                     DTTHYLRINKAFLGDDALRLAKIEDSSSYKNDLYVKLEGWENDKALHTIVFDTITIAN
                     KDSGMWYNPYMLAFKGDGILDPDLEYRLFITNTKTGHEVKGKTRLIKPFIVKKPPAGG
                     KLTLFRGFNTPFAWANGVNARRYEPIIRFNYFEIPSGTKDTISKHLDYVLTTITSENL
                     EGNGDAEVSFSNDAFYDFIKNNLSDNYQGKRLCGTVDLIISAGGNEYETYLRINGPSS
                     SIVQDKPEYTNIENGLGLLSSRYKVIREKHLDARAEDEIIALDRKFVKNPNL"
     gene            1247..2068
                     /gene="panB"
                     /locus_tag="PK345_07860"
     CDS             1247..2068
                     /gene="panB"
                     /locus_tag="PK345_07860"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004585127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HPX34967.1"
                     /translation="MNKKEANVYKKITTHVLQEMKLKGEKIAMLTAYDFSMAGLLDQA
                     GIDVILVGDSASNVMAGHTTTLPITLDQMIYHASSVMRAVKRALVVVDLPFGTYQGNS
                     KEALNTAIAIMKLSGANAVKIEGGSEIIESVDRILSAGIPVMGHLGLMPQSIHKFGTY
                     VVRATEDDEAQKLIEDAHLLEKAGCFSIVLEKIPAKLGGQVTQEIRIPTIGIGAGSDV
                     DGQVLVLHDMLGITQKFSPRFLRRYHNLSEEIQGSVRAYIQDVKTQNFPNEREQY"
     gene            2106..2801
                     /locus_tag="PK345_07865"
     CDS             2106..2801
                     /locus_tag="PK345_07865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005840104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HPX34968.1"
                     /translation="MINEIIYEDNHIIIVNKLPGDLVQADVKEGKTLRDDVKTYIKEK
                     YQKPGGVFLGVVHRLDRPVSGAVIFARTSKALTRMNLLLRKQEIRKIYWAVVDQPPSK
                     TEDWIENWLRKDQAKNKSFVVNKETKGAHLAELHYKMLTSSHRYHLLEIELKTGRHHQ
                     IRTQLSNIGCKIKGDLKYGFPRSNPDGSIHLHARKLEFIHPVSKKNITVIAKTPDDPI
                     WNFFEKEMALNKN"
     gene            2834..>5159
                     /locus_tag="PK345_07870"
     CDS             2834..>5159
                     /locus_tag="PK345_07870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34969.1"
                     /translation="MITHPLKYGINCCEENKTRFIYQYYFYQSRFFSSTPISAYVIFL
                     LLFFLIFTPLNITAQYYSSGQEPASVRWRQINTENFKLVFPDGYEASATYIANTLEYA
                     AKLDTVSFSVRPGKIPVLIHNFTSISNGMVAWAPKRMELYTIPPQNTYGQEWFQQLAI
                     HEYRHVIQLSQMKQGLTRILTYLFGEQIAAGVLGLYIPFWFLEGDAVLAETMFSQSGR
                     GRLPSFSLPLRTQLTQKGAFSYDKSVFGSFRDFVPDHYVLGYHLVTKAREKYGVDIWN
                     HVLDKVAKNPFMIVPFSEGIRDITGLGKTKLYEDVMNELKQDWQNQLNKSILTPTDEI
                     AIPEKDVFTNYLRPYFTDYKTIIAEKSPFDDITMFVEIDSTGNETKIYTPGYYLPQSL
                     TYAGDVIAWAEREYDPRWQHRNYSVIKIYDRKTGKSQKITNKSRFFAPALSPDASRLV
                     VVDVTLDQRYALVIIETTTGKEIQRVTTSQNYYFTNPSWCEDGKKIVTIIVGNQGKAI
                     ALVNPENRTVDRLTNFSYTDISNPVMCDEKIYFTGAWSGVDNIYQLDIDSKKITMVVS
                     APFGITDPQISADGTTLIFANYSEKGFKTSQKKIDETQQVLFENFEYKDVELHDKLFL
                     NNTNIFDPEKIPEDTYPIKNYSKILNLFNFHSWGPLITDYQNSEFKPGISMLSQNLLS
                     SSVTVLGWEYNTNEQTGKYYFNFRYEGLYPAFGADLSYGKRKSVYTDTTGSKDYYWRE
                     TNLKSDISVPLRFVAGKYTSYLTPIIAFDYTQLDI"
BASE COUNT         1649 a          943 c         1022 g         1545 t
ORIGIN      
        1 ccatcgaaag taacatggtt gccgggcgaa gtcgaagcac agcagttggg tacttataag
       61 atgccgccat aatatgtttt gtcttcttct acaaattcgg atttttcaca aattttcgat
      121 ctaacgcaat tatttcatct tccgcacgag cgtcaagatg tttttcgcgt attactttat
      181 aacggcttga aagtaagcct aatccgtttt caatgtttgt atattcaggt ttatcctgta
      241 ctatgctact cgatggtcca tttatccgta aatatgtttc atattcgtta ccgccagcgg
      301 aaataataag atcaactgtg ccacaaagcc tttttccttg atagttgtcg gagagattat
      361 ttttaataaa gtcgtaaaag gcgtcattac tgaaactaac ctctgcatcg ccattgcctt
      421 caaggttttc ggaagtgatg gtggtaagta cataatcaag atgtttactt atagtgtctt
      481 ttgtgcctga ggggatttca aagtaattaa atcttatgat aggctcatat cgtcgggcat
      541 ttaccccgtt agcccaggca aatggcgtat taaaacctct aaaaagggta agcttgccgc
      601 cagccggcgg tttttttacg atgaagggct ttattaaacg ggttttccct ttcacctcat
      661 gaccagtttt agtgttggta ataaataatc ggtattcaag atcgggatct aaaatgccat
      721 ctcctttaaa tgccagcata taaggattgt accacatacc gctatcttta ttagcgatgg
      781 taatagtatc aaaaactatt gtgtgaagcg ccttatcatt ctcccaacct tcaagcttta
      841 catagagatc atttttataa cttgatgagt cttcaatctt tgctaaacga agggcgtcat
      901 cgccaagaaa agccttattg atgcgcaggt aatgtgtggt atccatctga cttaaaagac
      961 cataaactat tgttatctct ttccaatctg ttgtcagatt aacttctgtt tcgcaagaat
     1021 agaataaaga aaaaacgaaa gtcaggatga aaggaatagt aatttttcgt atcataaaag
     1081 taaatcaaac gtttaaaata atactctttg tgagtgtaaa tatctgtggc tataaaaaaa
     1141 catctaattt tgtaattcgt attcgatatg caccaaacgg cattatattt tatttatttt
     1201 gagtgttaat gataatgagg tagtcatact attaaaattt ttataaatga ataaaaaaga
     1261 agctaacgtc tataaaaaaa ttaccactca tgtacttcag gagatgaagt tgaagggtga
     1321 aaagatcgct atgcttaccg cttatgattt ttcgatggct ggtttgcttg atcaggcagg
     1381 aattgacgtg atccttgtgg gcgattcggc atcaaacgta atggctggtc atactactac
     1441 gcttccaatc accctcgacc agatgattta tcatgcatca tcggtgatgc gtgctgtaaa
     1501 gcgtgcactc gttgtggttg acttaccatt tggcacctat caaggcaact ccaaagaggc
     1561 gctcaacact gctattgcaa tcatgaaatt atcgggcgct aatgctgtga aaattgaagg
     1621 cggcagtgag attatcgaat cggtagatag gatactctcg gctggaatcc ctgtaatggg
     1681 acatcttggg cttatgccac agtccatcca caaatttggt acttatgtgg taagagctac
     1741 cgaagatgac gaagctcaga aacttattga agacgctcat ttgcttgaga aagcaggttg
     1801 tttctccata gttcttgaaa agatacccgc aaaacttggt gggcaggtaa cgcaggagat
     1861 cagaatacct actattggta tcggtgcagg tagtgatgta gatggccagg tgctcgttct
     1921 gcatgatatg cttggtataa cccaaaagtt ttcgccacgg ttcctacggc gatatcataa
     1981 cctaagcgag gagatacaag gttcagtaag ggcttatata caagatgtga aaacacagaa
     2041 ttttcctaat gaacgtgaac aatactaacc aatcaactgt agtattttaa aggcagtgga
     2101 ttgatatgat aaacgaaatt atttacgaag ataaccacat tataatcgtt aataagctcc
     2161 ccggtgatct ggtacaagca gatgttaaag aaggaaaaac attacgcgat gatgtaaaaa
     2221 catatatcaa agaaaaatat caaaaacctg gcggagtatt tttgggcgtg gtgcaccgat
     2281 tggatcggcc tgtgagtggg gcggtgattt ttgcacgaac gagcaaagca ttaacaagaa
     2341 tgaatctttt gctgcgaaaa caagagatcc gaaagatata ttgggcagtt gttgatcagc
     2401 ctccttcaaa aaccgaagat tggattgaga actggttaag aaaagatcag gcaaaaaaca
     2461 aatcatttgt tgtaaataaa gagacaaaag gagcccattt ggctgaactt cattacaaaa
     2521 tgttgacgag tagccaccgc tatcatcttt tggaaataga gctgaaaacc ggcagacatc
     2581 atcagataag aacacaactt tcaaatatcg gatgcaaaat taaaggcgac ttgaagtatg
     2641 gcttcccaag gtcaaatcct gatggctcga tccatttaca tgcccgaaaa cttgagttta
     2701 tccatcctgt gagcaaaaag aatattactg taatagctaa aacacctgat gatccgatct
     2761 ggaatttttt tgaaaaagag atggctctaa ataaaaatta aatagaaaaa gttagaatct
     2821 ataacacaac gaagtgataa cacatccgct gaaatatggt atcaactgtt gcgaagaaaa
     2881 taaaactaga ttcatttatc aatactattt ttatcaaagt cgtttcttct catcaacccc
     2941 gattagcgca tatgtgattt ttcttttact attttttttg atttttacac ctctcaatat
     3001 tactgctcaa tattatagca gcggtcaaga gccggcatcg gtaagatggc ggcaaattaa
     3061 tactgaaaat tttaaattgg tgtttcctga tggatatgaa gcctcagcta cttacattgc
     3121 caatacatta gagtatgccg caaaactcga cactgtttct tttagtgtaa gaccgggtaa
     3181 aattcctgtt ttaattcata attttacatc tatttccaac ggtatggtag catgggcgcc
     3241 aaagcgaatg gagctctata ctatccctcc gcagaacact tatggacaag agtggtttca
     3301 gcaattagcg atacacgagt accggcatgt aatacaatta tcgcaaatga aacaaggact
     3361 tacacgaatc cttacctatc tttttggcga gcaaatcgct gccggtgtat tgggattgta
     3421 tataccattt tggtttcttg agggagatgc cgtacttgcc gaaacaatgt tcagccaaag
     3481 cggtagagga cgtttgccct ctttttcttt acctctacga acacaactta cacaaaaagg
     3541 agcgttttct tacgacaaat ctgtttttgg ctctttccga gattttgtgc cggatcatta
     3601 tgttttggga tatcatctgg taactaaagc aagagaaaaa tatggcgtag atatctggaa
     3661 tcatgtcctt gataaagttg caaaaaatcc gtttatgata gtgccgtttt ctgaagggat
     3721 aagagatatt acagggcttg gcaagacgaa actttatgaa gatgtgatga atgaactaaa
     3781 gcaggactgg caaaaccaat taaataaatc tatccttacc ccaacggatg aaatagctat
     3841 acccgagaaa gatgttttta ccaattattt gcgcccctat tttactgatt ataaaactat
     3901 tattgcggaa aaaagccctt ttgatgatat tacaatgttt gtagagattg attctacagg
     3961 taatgagact aaaatttata ctcccggcta ctatttgccg cagtcgctta cttacgccgg
     4021 tgatgtgata gcttgggccg aacgtgaata tgatccaaga tggcaacata gaaattactc
     4081 cgtgataaaa atttatgaca ggaaaaccgg taaaagtcaa aaaataacaa ataaatcgag
     4141 gttttttgca ccggcccttt cgcccgatgc cagccgtttg gtagtggttg atgttactct
     4201 tgatcagcgt tacgctcttg ttattatcga aactactacc ggaaaagaga ttcagcgggt
     4261 tacaacatca caaaactatt atttcacaaa cccgtcatgg tgtgaagatg gtaaaaagat
     4321 cgttactata attgtcggaa accaaggtaa agccatagca ttggttaacc ccgaaaatag
     4381 aacagttgac cgattaacca attttagtta taccgacata tcaaatcctg tgatgtgcga
     4441 cgaaaaaatt tactttacag gcgcatggtc tggagttgat aatatttatc aattggatat
     4501 tgattccaaa aaaattacaa tggtcgtttc agctcctttt ggtattaccg atcctcaaat
     4561 atcagcagat ggcacaactt tgatatttgc aaactattcg gaaaaaggat ttaaaacctc
     4621 tcaaaagaaa attgatgaga ctcagcaagt cttatttgaa aactttgaat ataaggatgt
     4681 tgagctacat gataagctgt tcttaaataa tacgaatata tttgatccgg aaaaaatacc
     4741 tgaagatacc tatccgataa aaaactactc caaaatactt aaccttttta attttcatag
     4801 ttgggggcct cttattacag attatcagaa ttcagagttt aagccgggta tctctatgtt
     4861 gtcgcaaaat cttttgagtt cgtcagttac tgtgctgggc tgggagtata atacaaatga
     4921 acaaacggga aaatattatt tcaacttcag gtacgaaggt ttgtatcctg cctttggcgc
     4981 tgatttaagt tatggaaaga ggaaatctgt ttatactgat actaccggat cgaaagatta
     5041 ttattggcgt gagactaacc tcaaatcaga catttccgtt ccgcttcgct ttgttgccgg
     5101 aaaatataca agttatctca ctccgataat agcattcgat tacactcaac tcgatatag
//
LOCUS       DAOOAI010000120         5139 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_18484_length_5139_cov_5.927223, whole genome shotgun sequence.
ACCESSION   DAOOAI010000120 DAOOAI010000000
VERSION     DAOOAI010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18484_length_5139_cov_5.927223"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(174..392)
                     /locus_tag="PK345_07875"
     CDS             complement(174..392)
                     /locus_tag="PK345_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34970.1"
                     /translation="METIEITMKKGIISRNKCPICNSTSLKIIFNRSFNEELIKEYMN
                     VAYQGNADIEFLEDITFEIVKCSECNLL"
     gene            complement(392..535)
                     /locus_tag="PK345_07880"
     CDS             complement(392..535)
                     /locus_tag="PK345_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34971.1"
                     /translation="MKDKLATTHNIANKGFIGMRRFVARFNVLCTLIGNRPQSLTGHI
                     VKR"
     gene            complement(550..1167)
                     /locus_tag="PK345_07885"
     CDS             complement(550..1167)
                     /locus_tag="PK345_07885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34972.1"
                     /translation="MRHLKILTIIILTLICAHGFGQIDDDNEIISALIHSEIKSLPND
                     TIFNRKGEIKKIKTYKKPDIILVIETETFLFDPEVNSFDSFKNETNGLPSLDSLTYRD
                     FIDKNKTKLQIDSIYGFQGTISYISRQEIEDIFKLGGWDNYHTTFGFKPLVKVSRPGI
                     NNDNSKAFIYYSSSTDGLGGAGFYLILEKLDGKWIVKESMLAWIS"
     gene            complement(1447..4278)
                     /locus_tag="PK345_07890"
     CDS             complement(1447..4278)
                     /locus_tag="PK345_07890"
                     /inference="COORDINATES: protein motif:HMM:NF022985.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Omp28-related outer membrane protein"
                     /protein_id="HPX34973.1"
                     /translation="MVKKYLYLLILLVAGYTVSGFTQPFFPGNNLHQTTQKSWHARSI
                     TELPFIATVNNRQDGQQVLKSFKNLPINASDNTVLKLQKYVVLEESTGTWCQWCPGGI
                     YYGDSLSRSYENVYVISVHGSDMMENTEHMNGTGLTAYPSANIDRNYTGKGISEWFSS
                     VNEALQSVPVASITVVNTFDTVTKELSVTVSATFSQNVSGDYRLGAVIVEDGVTGPQP
                     QYNQSNSYSGGGYGPMGGFETLPFFIPANMIAYNCVSRQLLGGYAGEPESLPELISAG
                     ETHDYTFSYILPEDRDEDLVYVIAWLIKPDGKIDNAIKSPYLNGNDNAKPVFLSNPIT
                     EAYVNSNYSYGIFVHDPDNQGLTIVDTQLPEWLSLSSTISVGHIHDKAILSGIPDSPG
                     DYEVILTVSDGENISIQNFIITVEEEMSGSWDLIGEAGFTTGQSQNYDLAVADDGTLY
                     LITNENDYLEVYKKEEGSNWVSIGLHQLKGWFGQIEIASDGSLYIAYSYDFNDVYVKR
                     YNGITWEQIGSSPTSGVQLGLVLDSEDHPIIVLQDFNNNSKGLAYIYDGINWSVLGGT
                     AYSTVGLAGVWNVLRIDENDIVYVLWGAFDYSGTPAYVSKYDGSNWVIVGDGPVGEAN
                     VYYYPTFDIDDNGYVYVAYPAGSATKSLETFKFTGTSWINTGSNLVGGATEHCKMRAD
                     VNGNIILAFSDISYNGNISALNYDGNDWTFIGPRGFTGTGGNYPVLDVMGETPYVGYS
                     DLLQDGKATVRAYLKQQIAVIHINPSEIVFDTTVVNYTSEAALFIVNQGNAVLEVTNL
                     NSSNNVFTANVNQLTIEPGDTATVAITFAPTLEQWYTGTIAVQSNDPVSGYLEIPVSG
                     YGIIENAVNELSEPNIIWPNPASEMIYVHYPTAISKIQMINYSGQAMYEESVDGFSTE
                     ISVKGFRPGVYFIKIFTIERVITEKVVIK"
BASE COUNT         1492 a         1054 c          935 g         1658 t
ORIGIN      
        1 aaagaatatt tttgaaaaat aaatcttgta atgctctcca aacttaaaac tgcgatagct
       61 gttgtaggca gttcacgggg tgatttttcc agcttacacc accaccattt tagcttttaa
      121 acttacactg acgacttata ttccctcacg gaaggtgatt tgttaaggat tgactataac
      181 aggttacatt cgctgcattt tactatttca aaggtgatat cttcgagaaa ttcgatatct
      241 gcattacctt gatatgccac attcatatat tcttttataa gttcctcatt aaaactgcga
      301 ttaaaaatta ttttcaatga agttgaattg caaataggac atttgttcct tgaaataata
      361 ccttttttca tagttatttc aatagtttcc attaacgttt gacaatatgg ccagtaaggg
      421 attgcgggcg atttcctatc aatgtacaca aaacgttgaa gcgggctaca aaccttcgca
      481 tgcctatgaa acccttattg gctatattgt gtgttgtggc cagtttatct ttcatattct
      541 gtcatttaac taagaaatcc atgcaagcat tgattcttta acaatccatt taccatctag
      601 tttttcaaga atcaaataaa atccagcccc accaagtccg tctgtcgaac tgctataata
      661 gataaaagct tttgaattat cattatttat tccaggtcgt gaaactttta caagtggttt
      721 aaatccaaat gttgtgtgat aattatccca tccccctagc ttgaatatat cttctatttc
      781 ctgtcgggat atatatgaaa ttgtcccctg aaaaccgtaa attgaatcaa tttgaagctt
      841 ggttttgttt ttgtcaataa aatcacgata agtcaagctg tcaagtgaag gtagtccatt
      901 agtttcattt ttaaaactgt caaatgagtt aacttccggg tcaaataaaa aagtctccgt
      961 ttcaatgact aaaataatgt ctggcttttt gtaagtcttt atttttttta tttctccttt
     1021 tctattaaaa atagtatcat taggcaatga tttaatctcg ctatgtatca aggctgaaat
     1081 tatctcatta tcatcgtcga tttgtccaaa tccgtgagca caaatcagag tgaggattat
     1141 aattgtcaat attttcaggt gtctcattgg tgtcttttta aattggcaac aacgtataat
     1201 ttacgtaaca aagttacaaa attcgagcaa aaagcaaaaa aaagaataat ggaaagaaca
     1261 tttttgaaaa ataaatcttg taatgctctc caaacttaaa actgcagtgg atgttgcaag
     1321 caattcacga ggcgaatctt tctgttcacc ccacacctac ttttactctt aaacttacac
     1381 tgatgactta tattccctca cagaaggtga tttgttaagg attgactcaa tagcgtggtt
     1441 tgtcatttac ttaatcacca ccttctccgt tatcacccgt tcgatagtaa atatctttat
     1501 aaaatagact cctggccgaa atccttttac tgatatttcc gttgaaaatc catccacact
     1561 ttcctcatac atcgcttggc ctgagtaatt gatcatttga attttgctga tagctgtcgg
     1621 gtaatgaaca tagatcattt ctgatgccgg attgggccag atgatattag gttctgacag
     1681 ttcattgact gcgttttcta taattccata accggataca ggaatctcga gataaccgga
     1741 tacggggtcg ttactttgaa cagcgatggt gccggtgtac cactgctcca atgtaggagc
     1801 aaaagtgatg gctacggtgg cagtatcacc tggttcaatg gtcagttgat tgacattagc
     1861 cgtaaatacg ttgttgctgg aatttaaatt tgttacttcc aatacggcat ttccctgatt
     1921 tacaatgaaa agagcagcct cgctcgtgta attaaccacc gtggtatcaa aaacaatttc
     1981 tgaaggattt atgtgaatca cggctatttg ttgtttcagg taggctctta cggtagcttt
     2041 tccatcctgt aataaatctg aataacccac ataaggcgtt tcacccatta cgtcaagtac
     2101 cgggtaattt ccgccggttc cggtaaatcc tcgcgggcct ataaacgtcc agtcatttcc
     2161 gtcatagttt agcgctgaaa tattaccatt ataactgata tccgaaaatg ccaaaattat
     2221 attaccattt acatccgctc tcattttaca gtgctctgtg gcacctccga ctaaattgct
     2281 gccggtgttg atccaggaag ttccggtaaa cttaaatgtt tccaacgatt tggtggcgct
     2341 tccggctggg taggctacat aaacataccc gttatcatct atatcgaaag ttggataata
     2401 atacacgttg gcttcaccaa ccggaccatc gcccacaata acccagttgc tgccgtcgta
     2461 ttttgacaca taggccgggg taccactgta atcaaaagcg ccccacagga cataaacaat
     2521 gtcgttctca tcaattctca gcacattcca gacacctgcc aggccaacgg ttgaataagc
     2581 tgtgccaccc aacactgacc agtttatgcc gtcataaata taggctaatc ctttggagtt
     2641 gttgttaaaa tcctgtaaaa caatgatcgg atgatcttcg ctgtcgagaa ccaatcccag
     2701 ctgcacgcct gatgtgggcg agcttccgat ttgttcccag gtaatcccgt tgtatctttt
     2761 cacgtaaaca tcattgaagt cataagaata cgctatgtaa agagagccgt cggaagcaat
     2821 ttctatctgg ccgaaccaac ccttaagctg atgcaaaccg attgataccc agttactgcc
     2881 ttcttccttt ttataaacct ccagatagtc gttttcattt gttattaaat acagggttcc
     2941 gtcatcggca accgctagat catagttttg cgactggcct gtagtaaatc ctgcctctcc
     3001 gatgagatcc caggaacctg acatctcttc ttcgacagta atgataaaat tttgaatgga
     3061 gatattttct ccgtccgaaa cggttaaaat gacttcataa tcgccagggc tgtctggaat
     3121 tcctgataaa attgctttat catgaatatg cccgactgaa attgtggacg acagacttaa
     3181 ccattccggc aactgtgtat ctacaatagt taatccctga ttgtcaggat catgcacaaa
     3241 aataccatat gaataattag aatttacata tgcttcggta atcggattgg atagaaaaac
     3301 cggtttagca ttgtcgtttc cgttcaggta aggacttttt atggcgttgt ctatcttacc
     3361 gtcgggtttg atgagccagg caattacata aaccaggtct tcgtcccggt cttcgggtag
     3421 tatatatgaa aaagtatagt catgcgtttc gccggcgctt atgagttcgg gcaagctttc
     3481 aggctctccg gcatacccgc ccagtaactg tcttgaaaca caattgtagg caatcatatt
     3541 ggctggtata aaaaaaggca gcgtctcaaa accgcccatt ggcccgtaac cgcctccact
     3601 gtaagagttg ctctgattgt attgcggctg cggacctgtt acaccgtctt ctacaattac
     3661 tgcccctaag cggtaatcgc ctgaaacatt ctggctaaaa gtagctgata cggtaaccga
     3721 taattctttt gtaacagtgt caaatgtatt aactactgtg atactggcta ccggtaccga
     3781 ttgtaaggcc tcatttacgg atgaaaacca ctcactgatg ccttttccgg tatagttccg
     3841 gtctatgttg gctgatggat aagcggtcaa tccggtgccg ttcatatgct ccgtgttttc
     3901 catcatatca gatccgtgaa ccgatataac atatacattt tcatagctcc gggataaaga
     3961 atccccataa taaatacctc ccggacacca ctggcaccaa gtacctgtac tttcttcaag
     4021 taccacgtac ttttgtaatt tcaaaacggt attatctgag gcattaatcg gcaggttttt
     4081 aaagctttta aggacttgtt ggccatcctg ccggttattt actgtggcta taaaaggcaa
     4141 ctcagtaata cttcgtgcgt gccatgattt ttgagtggtt tggtgcagat tattgccagg
     4201 aaaaaatggc tgggtaaaac cggaaacggt atatccggct accaggagaa taagcaagta
     4261 taaatatttt ttcaccataa aagtaaattt ttagcaaaag taaaatgttt ttaaaagtta
     4321 gacatattat taagcgtttt tacaaaaaat aagagatgct aaagctctaa tgatcgttta
     4381 attggaagat tctggctgat tagaaattct atggttcttt ttttattaca ccaccatccg
     4441 acaaaaacac aaaatcaatt ttaaccagag ttaattaatt gatcatcact aattgaattt
     4501 tgaattatca gcgaaaaaca cttacccgaa aagtaagcct tcgaaacatt aaaaccccat
     4561 aataatcttc ccccgaagga acggggacac caacttaaat tgcgcagcag cgggtgggca
     4621 ttaattttaa ttaatcgttt aataaatcaa tgcattgatg gccgcagccg ggatcaactt
     4681 ccgcatatta ccccgcaaac tataggcaga ggttattttt ttcttttttc aacttcaaaa
     4741 aattttccac ccaaaaccca ctttggcttt taaacttaca ctgatgaact taaattcctt
     4801 cactgaaaat gggttggttt gatcaattag atttcttgtt gcgatgtgtt tttattaaag
     4861 ttcaacttta ttgctgtttt ttagtttaaa agtctgtcct gctatttttg ctaacggacg
     4921 gcgtttatgt gtagtagcgg attgcgagtt tgtaatcatc atttactacc gatattttta
     4981 cgaagtgcaa gcctttatgg ttacatttat cccgccattg catatagcct atgttgtgcg
     5041 ttcagtcgtt tttatttttg tagttgtttt aatattttca taaaactttc tgctattaat
     5101 ttcttatcgg gtatatcaga aaatgctaac ggtgttaat
//
LOCUS       DAOOAI010000121         4759 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_20305_length_4759_cov_4.960247, whole genome shotgun sequence.
ACCESSION   DAOOAI010000121 DAOOAI010000000
VERSION     DAOOAI010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4759)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4759
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20305_length_4759_cov_4.960247"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            504..1415
                     /locus_tag="PK345_07895"
     CDS             504..1415
                     /locus_tag="PK345_07895"
                     /inference="COORDINATES: protein motif:HMM:NF017085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HPX34974.1"
                     /translation="MKRSSANNLSYPDLSEFFYDQVKVFRDYYHTTLIKFDRQAIHQM
                     RVAVKKINAVHKLKKSINYPITLRDTFFKLVKDIFFHSGKLRDVQLQHLILNNYKEKL
                     SYDFEHLQHHINKQEKCLLDNLQALVTHIEPTSFDKPSVLEKRVANAKYRVDPERDSI
                     AFIIKKTENISNLLLLIENEDYVHKLRKQVKQLFFVLQFLAHCFPDNVFGKYDLSNLR
                     MIGDRLGQWNDLFMFNLQINNFLGAQNVSFLEDFPEYHALQLLVRENRQSLLHGLDLL
                     IEQELLNLGLLISGNAPNTASSSLPEQ"
     gene            complement(1426..4710)
                     /locus_tag="PK345_07900"
     CDS             complement(1426..4710)
                     /locus_tag="PK345_07900"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HPX34975.1"
                     /translation="MQSKKDRLIWSINKVLQNAVNEIETEKLFNFVDWIFTLCQELSA
                     EHTSAVLDVILTLGKRIVDRDETEDRQYINYFEKKLIDFGFETPGIVYVNKDWQINVN
                     ENHIKNIRVWLELIEYPQTVMEKLLSALIVNLKLGGIFISDTDLFQREITKVLNSNIS
                     PFYKKVKELTRIFPVYFHEIGAEGEIRNVTTNMDEINERKDKLVHFLRKQVHTESNNT
                     LIDLTRRVFKFWCDGNKDALEPFVPKDVFDSIDLKSDYFITIHKLANKMLSMSAMTYA
                     TLLEVEPRIFEKLIERLPQEHQKDIHRLECIYALYALLKEKYSFETLDIISILRRYSF
                     IEASEITLFDNAMKEEDFDAALKLIYHFMEQLKKIIFNPETTQAWENIYHKRHIAIGI
                     PSMYGTYREPKFEAIGLTFRLENIATRLMEKIVDRINLEYVSAKTLKRILQILEFFKE
                     GLELDGINNQGFNSNLDMYRYSLTSGSFSLQQHINIFQFIAENVKVTIDKYFLRSYEY
                     PLKVIIPQLFFNGINYSEKEKMKIITMKSEEFFRDIISQAFLVQPLDNLISRVLNSLH
                     NMVDKYPSELINDVMSYNSDLVISPIYQETKNIDNQIFLGSKAFFLKKLYTSGFPVPP
                     GFVLTTEVFRRRNAISNHQEIKKEMNDLIMQHLQRLEKITGQEFGNSENPLLLSVRSG
                     TAISMPGAMDTFLNVGLNDEVTEGLSKKHNFGWTSWDCYRRFLQSWGMANGISRDVFD
                     DIMRTYKKRHDVDLKMYFKPETMKEIAMAYRKALDDHQVYFEQNPIKQLEYTINCVFD
                     SWDSDRAKVYRKHMNIANDWGTAVIVQKMILGNLNYTSGTGVVFTQNPHRQRPGVHLY
                     GDFKLCSQGEDIVAGLVTPLPVGETQRQYQQIEGESLQTKLPEIYKRIHKIAVEMTEK
                     HGYSPQEIEFTFESENPDDLYILQTRDQDFNQPAKINVFSHSSHQMKLVGRGIGIGGS
                     AMNGIVAIDNNDLIALNKNFPGEKLILVRPDTVPDDIGMIFECDGLLTAKGGATSHAA
                     VTAVRLGKTCVVNCTDMVVNDILKECVINEFVFKPGDKIAIDGYNGNIFKGHYPIETT
                     EIRTML"
BASE COUNT         1433 a          881 c          842 g         1603 t
ORIGIN      
        1 taggtaagat gggtaaaaag aagaaatctt tgcctaagga gggaataact ttcggcaaaa
       61 agccggttta aaaagtaatt agcgtgtttc ataagcgcct ccgcttagat ttttgtacaa
      121 ttgctgtaaa tgtgataaaa agagataaaa tctaaaaata tcgccagaga aataaaatta
      181 tatcccattt aaatcaacat ttaatctaac tgtagctgta attaaatgca taaaatttta
      241 tgtttctgaa aaatgaggtt aatgaaggtg tcttttcagt tttgaaaaag tattataagt
      301 gctaagatta gggccatcct ttcagatatt cattattttt tttagaatta cttttaatat
      361 gtacatggct taaactttat tgcagatcgc catttaaaaa ctttagaata aaaagcgatc
      421 tagaaaaaaa cataactaag aatggcaaat taagtaaaac ctgttgtttt tataataaat
      481 ctattagaaa gcgttttgta aatatgaaga ggtcatctgc caataatctc agttatcctg
      541 atttatcgga atttttttac gatcaggtaa aagtattcag agattattat catactaccc
      601 ttataaaatt tgacagacaa gctattcatc agatgcgtgt tgcagtaaaa aaaataaatg
      661 cggtacataa actaaaaaag agtattaact acccgataac tttacgcgat acttttttta
      721 aacttgtaaa agatatcttc tttcattcag ggaaattaag agatgtgcag ctccaacatc
      781 taattttaaa caactataaa gaaaaactta gctatgattt cgagcatctg caacatcata
      841 ttaataaaca agaaaagtgt ctcttagata atttacaggc attagttaca catattgaac
      901 ctacctcttt tgataagcct tcggtgttgg aaaaaagagt tgctaatgct aaatatcgtg
      961 ttgatcctga aagagatagc atagcgttta ttattaagaa aacagaaaat attagcaact
     1021 tacttttatt gattgaaaat gaagactacg tgcacaaact cagaaaacaa gtaaaacaat
     1081 tgttttttgt attacagttc ttggcacatt gttttccgga caatgttttt ggtaagtacg
     1141 atttaagtaa tttgcgtatg attggcgaca ggcttggtca atggaatgac ctgtttatgt
     1201 tcaatcttca gatcaataat tttttaggag cgcaaaatgt tagcttttta gaggattttc
     1261 ctgaatatca tgctcttcag cttcttgtgc gggagaatcg gcaatcttta ttgcatgggt
     1321 tggatttatt aattgaacag gagttgttaa atctgggttt actcatttcg ggcaatgctc
     1381 ccaatactgc ttcatcttca ctgcctgagc aataatattt tattattaca gcatagttct
     1441 tatttcggta gtttctatcg ggtaatgacc tttaaatata ttgccattat atccatcaat
     1501 tgcgatttta tcgccaggtt taaaaacgaa ttcgtttatt acacactctt ttaggatatc
     1561 gttaaccacc atatcggtgc aatttactac acatgttttt cctaatcgta ctgctgttac
     1621 ggcagcatgg gatgttgccc ctcctttggc tgtcagtagg ccgtcgcact cgaaaatcat
     1681 tccgatatcg tccggtacag tgtctggccg gacaagtatt aatttttcgc caggaaaatt
     1741 tttatttaaa gcgatcaagt cgttgttatc aattgctact attccgttca tggcgctgcc
     1801 tccaatccca atgccgcgtc caaccaactt catctgatgg ctcgaatgac taaatacgtt
     1861 aatttttgca ggctgattaa agtcttgatc acgtgtttgt aaaatataga gatcgtcagg
     1921 attttccgat tcgaatgtaa attcaatctc ttgtgggcta taaccatgct tttcggtcat
     1981 ctccacagct attttatgaa tccgtttata tatttccggt agttttgttt gcaatgattc
     2041 accctctatt tgttggtatt gtcgttgagt ttctccaacc ggtagcggtg ttacaagtcc
     2101 tgcaacgatg tcttcacctt ggctgcacaa tttaaagtcg ccgtacagat gcactcctgg
     2161 cctttgtcgg tgcgggtttt gagtaaatac cactcctgtt cctgaggtgt agtttaaatt
     2221 tccgagtatc atcttttgaa cgatcaccgc cgttccccag tcgttggcaa tattcatatg
     2281 ctttcggtac actttggccc tatcggaatc ccacgaatcg aaaacgcaat taatagtgta
     2341 ttcaagttgc tttatcggat tttgttcaaa atatacctga tgatcatcca atgctttacg
     2401 atatgccata gcgatctcct tcatagtttc aggtttgaaa tacatcttca ggtcaacgtc
     2461 atggcgtttt ttataagttc tcataatgtc gtcgaaaaca tccctactga taccgtttgc
     2521 catcccccag ctttgtaaaa accttcgata gcaatcccac gatgtccagc caaaattatg
     2581 tttcttgctc aatccttcag taacttcgtc attcaaacct acattaagaa aggtatccat
     2641 tgctccaggc attgaaatag ctgttcctga gcgtaccgag agcaataatg ggttttcgga
     2701 attgccgaat tcctgtcccg ttattttttc gagccgttgc aggtgttgca ttataaggtc
     2761 gttcatctct tttttgatct cctgatgatt ggaaatagca tttcgacgac ggaaaacctc
     2821 agtagttaac acaaaacctg gcggtacagg gaaccctgaa gtatatagct ttttaagaaa
     2881 aaatgcttta gacccaagaa atatctggtt atcaatattt tttgtttcct gatatatagg
     2941 gctgattacc agatcggagt tgtaggacat tacatcgttg atcagttccg aagggtattt
     3001 gtcaaccatg ttgtgaagcg aatttaaaac tctcgatatc agattatcta aaggctgaac
     3061 gagaaatgcc tgactgataa tatccctgaa aaactcctct gacttcatcg taatgatttt
     3121 cattttctct ttttcgctat agttgatgcc attaaaaaag agttgaggaa taattacctt
     3181 cagcggatat tcataagagc gcagaaaata tttatcaatt gttactttta cgttttcggc
     3241 aataaattga aaaatattaa tgtgttgttg cagcgaaaaa cttcctgaag tcaatgaata
     3301 gcgatacata tcaaggtttg aattaaaccc ttgattattt attccatcca attccaagcc
     3361 ttctttgaaa aactcaagaa tctgcaatat ccttttaagc gttttggccg atacatattc
     3421 aagattaatt cgatccacaa ttttttccat cagccttgta gcaatatttt caagtctaaa
     3481 tgtaaggcct attgcttcaa atttaggctc ccgatatgtt ccgtacatcg agggaattcc
     3541 aatggcaata tgtcgtttgt ggtatatgtt ttcccatgct tgagttgttt ccggattgaa
     3601 tatgattttt ttaagctgtt ccataaaatg atagatcaat tttagcgcag cgtcaaaatc
     3661 ttcttctttc attgcattat cgaaaagagt tatctcagat gcttcaataa atgagtatct
     3721 tctgagaatt gagataatat ccaaggtttc gaatgagtat ttctctttta gcaatgcata
     3781 gagcgcatat atacattcca gccggtggat atctttttgg tgttcctgag gtaggcgctc
     3841 aatcaatttt tcaaaaatcc gtggttctac ttcgagcaaa gtggcgtacg tcattgccga
     3901 catactaagc atcttatttg caagcttatg gattgtgata aaatagtcgc ttttcaggtc
     3961 aattgaatca aacacatctt tcggcacgaa aggctcaaga gcatctttat tcccatcgca
     4021 ccaaaatttg aaaacacgtc gggtaaggtc aataagtgta ttattacttt cggtatgcac
     4081 ttgcttacgc agaaaatgca ccagcttatc tttccgctca ttgatctcat ccatatttgt
     4141 agttacattt cggatttcgc cttcggcgcc gatctcgtgg aagtaaacag ggaaaattcg
     4201 cgtaagctct ttaacctttt tgtaaaaggg agatatgttg gaattgagaa ccttagtgat
     4261 ttctcgctgg aatagatcag tatcgctgat aaaaatccct cccaacttaa ggttgacgat
     4321 caaggccgag agaagttttt ccattactgt ctgcggatat tcgatcagtt caagccaaac
     4381 gcgtatattt tttatgtgat tttcgttaac attgatttgc caatccttgt tcacataaac
     4441 aataccgggt gtttcgaaac caaaatcaat aagctttttt tcaaaataat ttatgtattg
     4501 tctatcttcc gtttcatcgc gatcaacgat tcgtttaccc aaagtcaaaa taacatccaa
     4561 cacggctgaa gtatgctcag cgcttagctc ttggcaaagg gtaaaaatcc agtcaacaaa
     4621 attgaatagc ttctcagtct ctatctcatt tacagcattt tgtagcactt tattgatgct
     4681 ccatatcaat ctatcttttt tactttgcat gcctggcagg tgtaaaagat aaaatgtata
     4741 gaaaaactta tcaataaaa
//
LOCUS       DAOOAI010000122         4672 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_20762_length_4672_cov_6.718215, whole genome shotgun sequence.
ACCESSION   DAOOAI010000122 DAOOAI010000000
VERSION     DAOOAI010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4672)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4672
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20762_length_4672_cov_6.718215"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            121..351
                     /locus_tag="PK345_07905"
     CDS             121..351
                     /locus_tag="PK345_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34976.1"
                     /translation="MFICIGIKTVNFPIETCNRKYILTTRELLNGMLKKKYSDMTSYY
                     ASICVTYVKKINGKFVFSPANASLPYNPIEFE"
     gene            648..776
                     /locus_tag="PK345_07910"
     CDS             648..776
                     /locus_tag="PK345_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34977.1"
                     /translation="MSFKNPENNSIDTYWIKYFEKLGKTVPEDKLGTTNPAYITKS"
     gene            complement(929..1291)
                     /locus_tag="PK345_07915"
     CDS             complement(929..1291)
                     /locus_tag="PK345_07915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005854365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family protein"
                     /protein_id="HPX34978.1"
                     /translation="MKAILTIGLLICSNIFMTFAWYGHLKLAEYPWFNKLSLFGIIVF
                     SWGVAFFEYCFQVPANRIGFVSNGGPYSLVQLKVLQEVITLVVFVVFSYLVFQSQVKP
                     NHIIGFVLLVLAVYFIFK"
     gene            complement(1835..4234)
                     /locus_tag="PK345_07920"
     CDS             complement(1835..4234)
                     /locus_tag="PK345_07920"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX34979.1"
                     /translation="MKAIRLAARKLFRKGEHTTTRILSLATGLTFGLILLSEVFFYFS
                     VDSFYPDSKRIYVVNENFKADKQSDKMTTHPLVSGAIGPGLKAEVPGIEAATRLNSIG
                     TMVFYSEDNRGFNAHFVLADEHLPKVLPRPMISGLAEKILKTPMNCMVSDEIAQKMGG
                     NVVGKVIEINEFPGKKLTIGGIFKSLPENTNFSYDIAISMVSTAQFTWDGTENWLGND
                     RYYTCVKLEKAISPESLAPAVRKMQEKHQNIKELELKHGVVLKYSFEAIQKVFVNQQK
                     NILFILSAIAFIVLFVAVMNYMLLTLSTLAVRSKTLAIYKCYGAEKQNLHGMIFAESS
                     LNFILSLLVALALILLFKPIAEAQLGHSLQAALNRQVIVPLLLILVFLVFVISYFTGR
                     IFTKIPVAAAFRSYQQSKTKWKKALLAVQFISASLILTILVVVGLQFEKIKNTDHGYS
                     AENVYYTEVSGMDPHKIEAVLNDLIALPEVKNAGFGCDVPIYGAAGNNILSPDGERVL
                     FNLADFYYIDENYFSILDIPVIEGKGFEKEESSRQDVMISRNAAELLILNNGWHDGVI
                     GRSISITEHGDNFRISGIYPDIIEKLISADENRPSVFFYMPREKFIETLEADPSFSFL
                     ILIKTVEGNHPNIMNKFSEIINRAMPKGEIKLYSLEAEKDNAYRYHKSFRNAIYAGSL
                     VVLLVTVMGVLGYLNDEITRQRKNIAIRKINGATANDIIHFFIYRIGSIAVPCVIFGV
                     ATGFLLARSWMQNFPVQISLSWWIFVLAGGFVLLLTGVVTTMNSLKTASSNPIDSLRY
                     E"
     gene            complement(4314..>4672)
                     /locus_tag="PK345_07925"
     CDS             complement(4314..>4672)
                     /locus_tag="PK345_07925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HPX34980.1"
                     /translation="LWSILLALTGLLGLIIFISRDRIKEIGIRKINGASVIEIIRMLN
                     KNVLVWVSIAFVIATPIAYYAMQKWLENFAYKTTLSWWIFALAGLLALGIAQLAVSWL
                     SWKAATKNPVEALRYE"
BASE COUNT         1483 a          926 c          845 g         1418 t
ORIGIN      
        1 taagttcgaa tcttactacc ccgaccattt gtagcggatt tcaacatttg aggttcgctt
       61 tttttgtttt tcggtctgcc tggtctaatt cttaaagtgc atgaaagcaa aaatcattat
      121 gtgtttatat gtattggaat caaaacggtc aattttccca tcgaaacatg taacaggaag
      181 tatattttga caaccagaga gctgcttaat ggtatgctga agaaaaaata tagcgatatg
      241 acaagctatt atgcgtcaat atgtgtgact tatgtgaaga agattaacgg caaatttgta
      301 tttagtccgg caaatgcttc ccttccttac aaccctatag aatttgagta gaaaatgata
      361 acacaatgca tcactccaaa tctggtaaaa gatagtttag ttagtgcatc acaaattact
      421 tttgaggtaa cagatgtaaa caacttgact tgtgtttatt gaaaaaggca agacccacgc
      481 gtgttagaag caaaacctcc caaaggcgtg gtaaaaacgc ctaaaacaac ttgggtaaaa
      541 aataggagta catttacgaa gaaaccaaaa ataagtttta ttgcagcgag ttggcccgtt
      601 ttgcgttctt agattcgttc agaaatccaa tttctgagcc tttagcaatg tcattcaaaa
      661 atcctgaaaa caacagtatt gatacttact ggataaaata ttttgaaaaa ttgggcaaaa
      721 ccgttccgga agacaaactg ggaacaacaa atcctgccta catcactaaa tcttaaataa
      781 taaagatcgt ttacaaagat tatttagttc atttcgatta tcggtacagg actttgattc
      841 acatttattt acactattat ttataattgt agcgctcgaa ttaccaatct ttaataaata
      901 aggaaagctt tttcactaca aaattttatc acttaaaaat aaaatataca gccaacacta
      961 acagcacaaa ccctatgata tggttgggtt ttacctgcga ctgaaaaacc aagtaagaga
     1021 aaaccacaaa aaccacaagc gtaataacct cttgcagcac tttgagctgt accaacgaat
     1081 atggtccgcc gttgctaaca aaccctatcc tgtttgccgg aacctgaaag cagtactcga
     1141 aaaaagcaac gccccagcta aaaacaataa ttccgaacaa cgacagtttg ttaaaccacg
     1201 gatactctgc aagttttaaa tgtccgtacc acgcaaaagt cataaaaata tttgagcaaa
     1261 tgagtagccc gatagtaagt atagctttca taaaaatttt attactaata aaaacagcct
     1321 caaaagtatt aagtttgtgt caattctgca ggttggagta cgaaacatta ataggaagtg
     1381 gaattgccaa tgttatcggc atttataaaa caaaggtagt atctcataaa ctgtgtaagt
     1441 ttaaaatgaa gggttcagca tttatagttt tgcactgtat gaaaaggtag taaaaaaccg
     1501 attcagaaaa attattactt ttaatttttt aatgatgatg agctagtcta aaaaaatgga
     1561 tagaaaaata ataggctgat tacgaatcaa ctcacaaaag aacaaatgag atcaatgaca
     1621 ggggatattc gtactttata aagtgtgcac tgattaaaca cactgctaaa catagcgata
     1681 agccgacagc ccgaataatt gttctctaac ttcgccgcaa caaattctct atggcaagtt
     1741 ttaaattgta catcgcaatt gcaaattgct cattgctcat tgttaattgc tcattgctaa
     1801 ttgctcattg tccattgcaa attgctccca tcccttattc gtatctcagt gaatcaatcg
     1861 ggttactact ggcagttttc agggaattca tagttgtaac gacgccggta agtaataaaa
     1921 caaatccgcc ggcaagaaca aaaatccacc aagataagct gatctgcacc ggaaagtttt
     1981 gcatccaaga gcgggcgagt aaaaatccgg tagcaactcc aaaaatgaca caaggaaccg
     2041 cgatactccc aattctgtaa ataaaaaaat ggattatatc gtttgcagtt gcgccgttta
     2101 tcttgcgaat tgctatgttt ttacgttgtc gggtgatctc atcgttaagg tatccaagca
     2161 cgcccatcac ggtaaccaac aataccacta agctcccggc ataaattgca tttctgaaac
     2221 ttttatggta acggtaggca ttatccttct ctgcctcaag gctataaagc tttatctcgc
     2281 ccttaggcat agctctgttg attatctctg aaaatttatt catgatattc ggatgattac
     2341 cctccactgt tttaatcaag attaaaaaag agaagctggg atcggcctca agtgtttcga
     2401 tgaatttttc ccttggcatg tagaaaaaaa cggagggacg attttcatcg gcgctgataa
     2461 gcttttcaat aatgtcaggg taaatgcctg aaatcctgaa attgtctccg tgttcagtta
     2521 ttgaaatgct gcgtccaata actccgtcgt gccatccatt gttcaagatt aacagttctg
     2581 cggcatttcg acttatcatc acatcctgcc ggctcgactc ttctttttcg aaaccctttc
     2641 cttcgataac ggggatatct aaaattgaaa agtagttttc atcaatataa tagaaatcgg
     2701 ctaagttgaa cagtacccgt tccccgtcag gtgataatat attgtttcca gcagctccgt
     2761 aaataggtac atcacaaccg aatccggcat ttttaacctc aggcagtgct atcagatcat
     2821 tcagtacagc ctctatctta tgaggatcca tgccgctcac ttcagtataa tagacatttt
     2881 ccgcgctgta gccgtgatcc gtatttttaa ttttttcaaa ctgcaaacct acaaccacca
     2941 atattgtcag aatcagagat gcacttataa actgaacggc aagcagcgct tttttccact
     3001 ttgtttttga ttgctgatag cttcggaaag cagctgccac aggtatttta gtaaatattc
     3061 gtcctgtaaa ataactgatt acgaaaacta aaaacacaag tatgagtaaa agaggaacga
     3121 ttacctgcct gttaagagca gcctgaaggc tatggccaag ttgagcttcg gcaatgggtt
     3181 tgaacaacag tataagcgcc aaagctacaa gcagtgagag aataaaattt agggagctct
     3241 cagcgaaaat cattccatga agattctgtt tttctgctcc ataacattta taaatggcta
     3301 aagtctttga acggacagct aaggtgctta atgttagaag catgtaattc atcacagcaa
     3361 caaaaagcac aataaatgcg attgcgctaa gaatgaataa tatattcttt tgctggttta
     3421 caaatacttt ctgaattgct tcaaacgaat attttaaaac tactccgtgc ttcagttcta
     3481 attctttaat attctgatgt ttttcttgca ttttgcgtac agccggtgca agagattccg
     3541 gtgatattgc cttctctaat ttcacgcagg tataatagcg gtcgtttcct aaccagtttt
     3601 ccgttccatc ccatgtgaat tgagctgtgg ataccattga aatagcaata tcatatgaaa
     3661 agtttgtgtt ttcgggcagt gatttaaaaa tgccgccaat ggtgagtttc tttcccggaa
     3721 attcatttat ttcaattact tttcctacta cattaccacc catcttttgg gctatctcat
     3781 cggaaaccat acagttcatg ggtgtcttta gtatcttttc ggccagacca ctgatcatag
     3841 ggcgcggtaa aactttaggt agatgttcgt cagctaaaac aaaatgagca ttaaagcctc
     3901 ggttatcttc ggaataaaat accatagtac ctattgagtt gagccttgtt gcagcttcaa
     3961 tacccggcac ttctgctttt aatccgggac caatagcgcc acttaccagc ggatgggttg
     4021 tcatcttatc agattgtttg tctgccttga aattttcatt tacaacataa atgcgttttg
     4081 aatcaggata gaagctatct acactaaaat agaagaacac ttcggacaat aagataaggc
     4141 caaaggttaa tccggtagca agagagagaa tacgcgtggt ggtatgttct cctttcctga
     4201 atagcttccg tgcggctaat ctgattgctt tcatatttcg attttaattt gattttttaa
     4261 tatttagtga taaattattc gtattgttag attggtctat ttttttttga accttattca
     4321 tatctcaatg cctccacagg attttttgtt gctgctttcc aacttagcca actcactgca
     4381 agctgtgcta ttcccaatgc taacaagccg gcaagggcaa aaatccacca gcttagcgta
     4441 gtcttgtagg cgaagttttc caaccatttt tgcatggcgt aatacgcaat gggcgtggct
     4501 attacgaaag cgatgcttac ccacaccaac acatttttat ttagcatacg tattatttcg
     4561 attacggaag cgccgttaat ttttcgtatg cctatctctt taattctgtc gcgtgatatg
     4621 aagataatca accccaacag tccggtcaat gctaatagaa tgctccacag tg
//
LOCUS       DAOOAI010000123         4653 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_20875_length_4653_cov_4.844498, whole genome shotgun sequence.
ACCESSION   DAOOAI010000123 DAOOAI010000000
VERSION     DAOOAI010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4653)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4653
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_20875_length_4653_cov_4.844498"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="PK345_07930"
     CDS             complement(<1..816)
                     /locus_tag="PK345_07930"
                     /inference="COORDINATES: protein motif:HMM:NF013761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HPX34981.1"
                     /translation="MIEFDNTEIAFRNKSNYDLRKSFLLFSALSRNWLMKLGVGLTQS
                     FMRIGLPVNWFVKPTIYKQFVGGETITDSIPVVEMLGKYNVKAILDYSVEGSDSKEGI
                     QNALEETLRSIENAANHSNIPFAVFKPTAFTTSTVLLKVSAGDELTANEKEEADNFRK
                     RVDKLCSAAFEKGIPILIDAEDSWYQNFIDDVVNDMMAKYNKERTIVFNTFQMYRHDR
                     LDYLKKSFQMALEGNYFLGAKFVRGAYMEKERERAAQLGYPSPIQPDKEHTDND"
     gene            1021..1833
                     /locus_tag="PK345_07935"
     CDS             1021..1833
                     /locus_tag="PK345_07935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34982.1"
                     /translation="MKLKVYQIINILGLLIMIVAYYMANSLPIGGRHTFEIFDLYPTL
                     FTPARFTFSICWLIYLLLFLFIIYQSKGLFSNSNLKRNRFLYRIDIWFFISSIASVAW
                     IIAWHHNLIWVTVVLVGVVLAALSTIYFYLGVGRESISRAEYYMVHVPFSIYLGWVAV
                     LAITNVAIFLVSTHWYGFNLSDQFWGTLMVALTGTLGVYVVLFRKDFYYGFTIIWALI
                     GIISRKLADHNESSESIIIAAGAGILIILLLSIFFCLKKIWKNRTNPIKTIN"
     gene            1845..3041
                     /locus_tag="PK345_07940"
     CDS             1845..3041
                     /locus_tag="PK345_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013616122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1343 domain-containing protein"
                     /protein_id="HPX34983.1"
                     /translation="MNSFFSILKISSLLVFIILQTNCTNGQSTHEIQPGACQTSSYFP
                     MLQGKRVAIVANHTATIDDVHLVDSLVRAGIEVKKVFSPEHGFRGIAEAGQKVDDAID
                     LVTGLPVLSLYGNNKKPRKEDLSDIDLIIFDIQDVGARFYTYISTMTYVMEACAEHGI
                     PLIILDRPNPNGFYVDGPVLQPEFKSFVGLHPVPIVHGMTIGEYAQMVNGEGWLENEL
                     KCEIKIIPVKNYMHSDFYELPLRPSPNLPNKNAVYLYPSLCLFEGTVVSVGRGTELPF
                     QVIGHPEFTKGNYYFVPKPMIGAVKPPFDNITCNGFNLTDWAQKMNENSAQINLDWLI
                     LFYNELSEKNQFFNSFFEKLAGTAELRKQIEQGLSAEQIRETWQPELKEYKRIRKKYL
                     IYTDIE"
     gene            3050..>4653
                     /locus_tag="PK345_07945"
     CDS             3050..>4653
                     /locus_tag="PK345_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34984.1"
                     /translation="MVRIGSKWLTLKELLIRFAPEFILIIYLLLFFVVKNPSNPHDRV
                     IISDGKSYYAYLTTAFIYHDLNYNFVEYYESKYYPDHPSIFKEFRYTFKEQIVNKAFP
                     GLAFLLLPFFIVAHLIALLFGLPADGYSLIYQYFMGFAALFYFWAGLRYTKKLLTASG
                     FNQKVAAVILVLIAFATNIIYYTLKEGLMTHVYNFFLLATFLYYLKDFTKNHKANSLI
                     IAALVYGLIVSVRPTNALFALLIPFVLGSSASVKSLFNTLFAEKRLLLKVVAAGLVFP
                     IITMIIWYLHSGYWFVWSYGDEGFDFAKFNFLKILFSFNKGWFVYTPLALFSMTALIW
                     FFRNQFYRFLWLTVFWILFIYVASSWWVWTYTSNFGLRTFIDTYALIALMLGYVWLLI
                     RDSKILIVFAKILGIALIVLNSLQFYQHYTYVFPPGEITSSIYKESFFRLKPKPKVYY
                     PEGYITSRHTFYNDFEKRYGWANEESITDEKAYEGNFSSKTGLSGVYSIGLWQSLDSC
                     FTTEYNRIRVSSWIFSDKAKSSGVLV"
BASE COUNT         1383 a          830 c          860 g         1580 t
ORIGIN      
        1 atcattatcg gtgtgctctt tatcgggctg aattggtgag ggatagccta actgtgctgc
       61 ccgttcgcgc tctttctcca tgtaagcgcc acgaacaaat tttgccccta gaaaataatt
      121 accttcaagc gccatctgaa aggatttttt taggtaatca agacgatcgt ggcgatacat
      181 ttgaaaggtg ttgaagacga ttgtacgttc cttattgtat tttgccatca tatcatttac
      241 cacatcatca ataaaatttt ggtaccacga atcttcagca tcaatcagga tagggatccc
      301 tttctcaaaa gcggcgctac aaagcttatc aactcgtttt ctaaaattat cagcctcctc
      361 cttttcatta gcggtaagtt catcgcctgc actgactttt aaaagtacag tacttgtagt
      421 aaaggctgtc ggtttaaaca ctgcaaaagg aatgttggaa tgattggcag cattttcgat
      481 tgagcgaagc gtctcttcca aagcattttg tatgccctct tttgaatcac ttccttcaac
      541 ggaataatca agtatagcct tgacattata tttaccaagc atttcaacaa ccgggatact
      601 atcagtaata gtttcgccac caacaaattg tttgtaaatg gtcggtttta caaaccaatt
      661 aacaggtaac cctatccgca taaaggattg tgtcagacca acacctaatt tcatcagcca
      721 attcctggaa agagcgctaa aaaggagaaa agacttcctg agatcgtaat tactcttgtt
      781 cctgaaggct atttcagtat tatcaaattc gatcataact tttttttcac aaaagtaatg
      841 aagcaaatta atttgttgct tataccgttg taaattttac ttattatttt tgtacaaaat
      901 caaacaaagt tgtttttctg gtaacaagac ttttttgaca attaatagaa tagcatttta
      961 aagaaaagtg ttttgataaa aagtttgaat agttttttat tgcattaaca aaaattccaa
     1021 atgaaactta aagtatatca gataataaat atccttggcc tattaattat gatcgtagca
     1081 tattatatgg ccaattcact acctattggt ggtaggcata cttttgagat ttttgatctt
     1141 tatcctaccc tattcactcc ggcacgtttt actttttcca tttgttggct gatctatctt
     1201 ttacttttcc ttttcattat ttaccaatca aaaggacttt tctcgaatag taatctaaaa
     1261 aggaaccgat ttctttatcg tattgacatt tggtttttca tctcaagcat tgcaagtgtt
     1321 gcatggatta ttgcctggca tcataactta atatgggtaa ctgtggtttt ggtgggtgtt
     1381 gtacttgcgg ctctgagcac catttatttt tatcttggag ttggcagaga atcaataagt
     1441 cgggctgaat attatatggt tcatgtccca tttagcatat acttaggatg ggtggcggtg
     1501 ttagcaataa caaatgttgc tatttttctg gttagtacgc attggtatgg ttttaatctt
     1561 agcgatcagt tttggggaac attaatggtt gccttaaccg gaacattagg tgtgtatgtt
     1621 gtgttgtttc gaaaagactt ttattatggt tttacaatta tatgggcgct tattggaatc
     1681 atatcgcgaa agctggccga ccataatgaa tcttccgagt caatcataat cgctgcaggc
     1741 gccggtattc tgataattct tttactgagt atcttttttt gccttaaaaa aatttggaaa
     1801 aacaggacaa atccaataaa aacgattaat tgattatccc tatcatgaat agtttttttt
     1861 cgattttgaa aatctcttct cttttagttt tcataatact tcaaaccaat tgtactaacg
     1921 gccagtcaac acatgaaata caacccggcg cctgtcaaac ttcatcgtac tttcctatgt
     1981 tacagggaaa aagagttgct attgtggcta atcacacagc aacaattgat gacgtgcatc
     2041 tcgttgacag ccttgtcagg gcagggattg aagtaaaaaa agtctttagc cccgagcatg
     2101 gttttcgagg tattgccgaa gctggacaaa aggtggacga tgctattgat cttgtaacag
     2161 ggttgccggt attatcattg tatggaaaca ataaaaaacc acgaaaggag gatctgtcag
     2221 atattgattt gataatcttt gatattcagg atgtaggcgc acgattttac acttatattt
     2281 ccactatgac gtatgtaatg gaagcttgtg ccgaacatgg tatcccactg attatcctcg
     2341 accgccctaa cccaaacggc ttttatgtgg atggaccggt gttgcaacca gaatttaaat
     2401 ctttcgtagg actgcatcca gtaccaattg tacatggcat gacaataggc gaatatgcac
     2461 agatggtcaa tggtgaagga tggttagaga atgagttgaa atgtgaaata aagataatcc
     2521 ccgtcaagaa ctatatgcac agcgattttt acgaactgcc cttaaggcca tccccaaatc
     2581 tacctaataa aaatgcagtc tatctttatc cgtctttatg tcttttcgaa ggaactgtag
     2641 taagtgttgg acgaggcacc gagctgccat ttcaggtaat aggacaccct gagtttacta
     2701 aaggcaacta ctatttcgtg ccaaagccta tgatcggcgc agtaaagcca ccatttgata
     2761 acatcacttg caacggattt aaccttacag attgggctca aaaaatgaat gaaaacagcg
     2821 ctcaaataaa tcttgactgg ttaatcttgt tttataacga gctctcagaa aaaaatcagt
     2881 tttttaattc tttttttgaa aaacttgccg gtaccgccga attaagaaaa cagatcgagc
     2941 agggattaag cgctgagcaa ataagagaaa catggcaacc agaacttaaa gagtataaaa
     3001 ggataagaaa gaagtatctt atttacactg acattgaata aaaaaagtaa tggtgaggat
     3061 tgggagtaaa tggttaactc tcaaagaatt attaatcagg tttgcgcctg agtttattct
     3121 aataatatat ctgttattgt tttttgttgt aaaaaatcca tccaatcctc acgacagggt
     3181 cattatcagc gatggaaaaa gttattatgc ctaccttact acagcgttta tttatcacga
     3241 tttgaattac aatttcgttg aatattacga gtcgaaatat taccctgatc acccttctat
     3301 ttttaaagag tttcgatata cgtttaaaga acaaattgta aacaaagcct ttcccggatt
     3361 ggcttttttg cttttaccat tttttattgt agcacacttg atagcgttac tttttggcct
     3421 accggctgat ggctattcat tgatttatca atattttatg ggctttgcgg cgctctttta
     3481 cttctgggcc gggcttcgct atacaaaaaa gttactcacc gcgtcagggt ttaatcaaaa
     3541 ggtcgcagcc gttattcttg tgttaatagc gtttgcaaca aatataattt attataccct
     3601 gaaagagggg ttgatgacgc acgtttacaa tttttttctt ttagcaacat ttctttacta
     3661 tctaaaggat tttacaaaaa accataaagc aaattcattg attatcgcgg cgttagtata
     3721 tggattaata gtatcagtaa gacctactaa tgccttgttt gcattgctaa tcccatttgt
     3781 tttaggatca tcagcttcgg tcaaatcact ttttaacacc cttttcgctg aaaaaagatt
     3841 acttttaaaa gttgttgctg ccggacttgt ttttcctatt attacaatga ttatctggta
     3901 tctccattcg ggttattggt ttgtatggtc gtatggcgac gagggatttg actttgcgaa
     3961 attcaatttt ttaaaaatat tattcagttt taataagggc tggtttgttt ataccccact
     4021 tgcattattt tcaatgacag ccttaatatg gttttttcgc aatcagttct atcgcttttt
     4081 atggctaacg gttttctgga tattatttat ttacgttgcg tcaagttggt gggtctggac
     4141 ttatacatcc aattttggac tacgtacttt tattgataca tacgcattaa ttgccttgat
     4201 gcttggctat gtgtggctac taattcgaga ttccaaaata ttgatcgtct ttgccaaaat
     4261 tttaggcata gcacttatag tactcaacag cttgcagttt tatcaacatt atacctacgt
     4321 ctttccaccg ggagaaatta cttcaagtat ttacaaagaa tcatttttcc gtttaaaacc
     4381 aaaaccaaag gtttattatc ctgaagggta tattacttca cgtcatactt tttataatga
     4441 ttttgaaaaa agatatggct gggctaacga agaatcaatt actgacgaaa aagcctatga
     4501 ggggaatttt tcttctaaaa cagggttatc aggtgtttac tctattggtt tgtggcaatc
     4561 gttagattcc tgttttacaa cggaatataa tcgaattaga gtgagttcat ggatattttc
     4621 ggataaagcg aaatcatcag gcgtattagt tat
//
LOCUS       DAOOAI010000124         4602 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_21160_length_4602_cov_4.684407, whole genome shotgun sequence.
ACCESSION   DAOOAI010000124 DAOOAI010000000
VERSION     DAOOAI010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4602)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4602
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_21160_length_4602_cov_4.684407"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1289)
                     /locus_tag="PK345_07950"
     CDS             complement(<1..1289)
                     /locus_tag="PK345_07950"
                     /inference="COORDINATES: protein motif:HMM:NF012999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HPX34985.1"
                     /translation="MKSKFYLFTLIYLFFSMLAINTHLQAGFNNKDIFITEISNQIVN
                     FTATATIIPVGNNVTFTDQSTGEPLSWSWSFPGGIPNSYTGKIPPPITYNTVGAYDVI
                     LTVTYPDNVIVSETKTNFIDVKNYPAGWSFTKTNLSHLISVPVSVNFPDTSMVYGDFI
                     GVFYLDDESVEQCGGAAVWDETNSKAVIAYGDDITTQTKDGFAAGDTLLWKVWSAAKN
                     EICTATVEYNTALPQNNGLFANNGLSSLTSVSFKTSAPLNVVATASPSPVCNGSPVQL
                     DVDVSGGLGDYSYQWSSNPAGFNSNQKNPLAYPIQETIYTVIVSDGSNQDTSSVTVQV
                     VQKPVAYLESDITICAGEQVQLTGSVDLNCGFQWITNGDGSFTNLEIFDPEYTPGMQD
                     ILNGSVEICLSALPCTPCSQPANACITITIINHAQVN"
     gene            1460..4330
                     /locus_tag="PK345_07955"
     CDS             1460..4330
                     /locus_tag="PK345_07955"
                     /inference="COORDINATES: protein motif:HMM:NF039201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX34986.1"
                     /translation="MRNVYFFLVIAALIVFPFISSAQYITPGNNLNLTLDELVNTSSG
                     VVTFVDGAYQINSAFTLSATDTLRIDQEAIVRIASGIRPQIYGTIISDPESGYVHFTA
                     IDTTSSSKNYKGFRFDDSPSNVFRNTIFTYGGGIQLIGTEALFEYCTFRKNGSSNVSA
                     VITYSSCSPIIRYCSFIENARAAIGSGANVNGSPRIMYNVFIHNTTDNSNRPQINLGP
                     GAADSIYIVGNYIEGFYTRAGGIGISNLMAIGSTKVVVRDNYVINNRFGYTQIGVNIF
                     GHIVDNYFIDNNIENSPALGGSGVNFLTSGEGNTAIVKRNVIKGNLWGATIQGNANPQ
                     FGTEDDPGENVFYENGNSGITYALYNNTPLNVTAIGNYWGTNDPNVAEDYIFHKPDDA
                     TLGLVTYMPIKTLEPKILTFLFSASENPQLTLDVYADINHDTHTINATLPPGTDVTVL
                     VPEIVVELGIITDPTGGQPTDFRLPVEYNVTTPHGDVAVYTVTVDVQPWDLKVTFIVL
                     DEENNPIGDAVITFNGVTNVAGDYQFAGLSPGDYDYTVELDGFITQTGQVEIIDQDVT
                     VTVVLEEIGQLVELKEGWSMISSYLVPEDNDLTVIFAKQTLAGNMVIMLNNAGIFWPG
                     QNINTLKTWNPYIGYKLKMTEDDFVNFKGLEVVDKTINLNKGINYLPVLTNAAIESAV
                     IFEQLDGQLILAFDINNNLIYWPEGGINTLLTLEPGSAYLLNLVGPASLTYPDVIDGK
                     IKTTKLPAITTANPWSIVKTGAYHIISIYETALTGFAAEDIIGAFSSKGQCVGMVQKY
                     DQTGNLGLVVYGNDFTTDEVDGLMQNEEIHFKVYRAATGEIEEIVPVWDNTKPNEGLF
                     VEYGLSAISQFKAEMTSVTITDKYNFSLYPNPAAESVNIVGSFPKFVRAFIVEPTGRI
                     VNQIELNGNFTKVDISQLKSGIYLIQLFDGDQLLFTEKMLVP"
     gene            complement(4414..>4602)
                     /locus_tag="PK345_07960"
     CDS             complement(4414..>4602)
                     /locus_tag="PK345_07960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX34987.1"
                     /translation="NGYEEKYIQAIESKKDEIEKATDLFITNLNILNNYSEKDKIIEE
                     EIQDFAEETTKETEASNE"
BASE COUNT         1382 a          813 c          904 g         1503 t
ORIGIN      
        1 atgttgactt gtgcatggtt tattatagtt attgtaatgc aagcattagc cggttgtgaa
       61 caaggcgtac agggcagtgc agacaggcaa atctctactg agccattgag gatatcctgc
      121 atgccgggtg tatattccgg atcaaaaatt tcaaggttag taaaactacc atctccattg
      181 gttatccact gaaacccaca atttaaatct actgatcctg tcagttgaac ttgttctccg
      241 gcacagattg taatatctga ctccaaataa gctaccggct tttgcaccac ctgaactgta
      301 actgagctgg tatcctgatt tgatccatca gacactatta cagtgtaaat agtttcctga
      361 atgggatatg ctaacggatt tttctgattc gaattaaaac ctgccggatt agaagaccat
      421 tgataagaat agtcgcctaa tcctccactg acatctacat caagttgaac aggagagcca
      481 ttacaaaccg gggatggcga tgctgtggct acaacgttta atggagccga ggtcttaaaa
      541 cttaccgatg taagtgaaga taaaccgttg ttagcaaata atccgttatt ttgtggtaac
      601 gcagtgttat attcaacagt tgcggtgcaa atttcgtttt tggcggcgct ccatactttc
      661 cataataaag tatcacctgc agcaaagcca tctttggttt gggttgtaat atcatcgcca
      721 taagcaatca ctgccttact gttggtttca tcccatactg ctgcgccacc acattgttca
      781 acactttcat catctaaata aaagactccg ataaaatctc catataccat gctagtatca
      841 gggaagttaa cactaacggg aactgaaata agatgtgata aattagtttt tgtaaatgac
      901 caaccggctg gataattctt tacatcaata aaattggttt ttgtctctga aacaataaca
      961 ttatcgggat acgtaacagt aaggattaca tcatacgctc ctacggtatt gtaagttatt
     1021 ggagggggaa tttttccagt ataggaatta ggtatccccc ccggaaatga ccaactccac
     1081 gataacggct ctccggtgga ttggtcggta aaagttacat tgttgccaac gggtataatg
     1141 gtggcggttg cagtgaaatt tacaatctga tttgatattt ccgtgatgaa aatatctttg
     1201 ttgttaaaac ctgcctgcaa atgtgtatta atagccaaca tgctgaaaaa taagtagatc
     1261 agcgtaaata aatagaattt agacttcatt attaatggtt gttttagaaa ttgcaaattt
     1321 agcttttgaa aaattaaggc taaaatatgt ttttttttgg attatttgat ttttaaagcg
     1381 ctctttttta tttgttcctg taattgtgtg aatcaaaagt ttatatttat atttgtagta
     1441 aatttaaaaa ccaaaaaata tgcgaaacgt ttactttttt ttagtaattg ctgctttgat
     1501 tgtttttcca tttatcagca gcgctcaata catcacccca ggtaataact tgaatctcac
     1561 tcttgatgaa ttagtgaata cctcaagtgg tgtagtaacc tttgttgacg gcgcctatca
     1621 gatcaatagt gcatttacac tatctgcaac ggatacattg cgcattgatc aggaggctat
     1681 cgtacgaatt gcttcaggaa tcagacctca aatttatgga accattatct cagatcctga
     1741 aagtggttat gttcatttta cagccattga taccacttcg tcttccaaaa attataaagg
     1801 cttccgcttt gacgattctc cttccaatgt atttcgcaat accattttta cttatggcgg
     1861 tggaatacaa ctcatcggta cagaagcgct ttttgagtac tgtacttttc gaaaaaatgg
     1921 ctcttccaat gtttctgctg tgattaccta tagttcgtgc agcccgatta ttcgctattg
     1981 cagttttata gaaaatgctc gtgctgctat aggttcaggc gctaatgtta acggctcgcc
     2041 tcgtattatg tataatgttt ttattcataa taccaccgac aacagtaacc gtcctcaaat
     2101 caatttaggg ccgggcgctg ccgatagcat ctatatagtt ggaaattaca tcgaaggatt
     2161 ctacaccagg gcaggcggaa taggtattag caatttgatg gctatcggta gcacgaaagt
     2221 cgtggtacgt gataattatg tcattaacaa ccgttttggc tatacacaga taggtgttaa
     2281 tattttcgga catattgtag ataattattt tattgataac aatattgaaa attcacccgc
     2341 tttgggtggc agcggtgtaa actttctgac aagcggagag ggtaatactg ctattgttaa
     2401 acgaaacgtt attaagggta acttatgggg cgctactatt caggggaatg ccaatcccca
     2461 atttggaaca gaagatgatc cgggtgaaaa cgtattttat gaaaatggaa atagcggtat
     2521 tacttatgca ttatataaca ataccccgtt gaatgttact gcaataggta attactgggg
     2581 aacgaatgat cccaatgtgg ctgaagatta tatctttcat aaacctgatg atgctaccct
     2641 cggacttgta acttatatgc cgataaaaac ccttgaacct aaaatattga catttttgtt
     2701 ttcagcttcg gaaaatccac aattgacttt ggatgtttat gccgatatta accatgacac
     2761 ccatactatt aatgccactt tacctcccgg tacggatgtt actgtactcg tgccggaaat
     2821 tgtggttgaa ttaggaataa taaccgatcc tacaggcggc caacctactg attttcgttt
     2881 accggtagag tataatgtta ccactccaca tggcgatgtt gcagtttata cagttacggt
     2941 tgatgttcaa ccatgggact taaaagtaac tttcatagta ttggatgagg aaaataatcc
     3001 cattggggat gctgtaataa cttttaatgg ggtaaccaac gtagcgggag attatcagtt
     3061 tgccggtctt tcgccgggag attatgatta cactgttgaa cttgatggct ttatcacaca
     3121 aaccggacaa gtggaaatta ttgatcaaga cgtaactgta acagtggttt tggaggagat
     3181 cggacaattg gtcgaactta aagaaggctg gtcaatgata tcttcatact tggttccgga
     3241 ggataacgat cttacagtca ttttcgcaaa acagacactt gcaggaaata tggtaataat
     3301 gcttaataat gccggtattt tttggccagg tcaaaatatt aatacattaa aaacatggaa
     3361 tccttacatt ggatataagt tgaaaatgac ggaggatgat tttgtaaatt ttaaaggatt
     3421 agaggttgtt gacaaaacca taaatcttaa taagggtatt aattatcttc cggttcttac
     3481 taacgcagct attgaatcgg ctgtgatttt tgaacaatta gacgggcaac taattttagc
     3541 ctttgatatt aataataacc tgatctattg gcccgaaggc ggtattaaca cgctattaac
     3601 ccttgagcct ggttcggctt atcttttaaa tttagttgga ccggcaagtc tgacttaccc
     3661 tgatgtaata gatggtaaaa ttaaaaccac gaaactgcct gcgattacta ctgctaatcc
     3721 gtggtcaatt gttaaaactg gcgcttatca tatcatttca atttatgaaa ctgcactgac
     3781 gggttttgct gcagaagaca tcatcggtgc ctttagcagt aagggacaat gtgtaggaat
     3841 ggttcaaaaa tatgatcaaa ccggtaatct tgggttggtt gtgtatggca acgactttac
     3901 taccgatgag gtggatgggc ttatgcaaaa cgaagagata cattttaaag tctatcgtgc
     3961 agctactggt gaaattgaag agatagtccc tgtttgggat aacacaaagc caaatgaagg
     4021 cttatttgtt gagtatggac tttcggccat tagccagttt aaagctgaaa tgacaagtgt
     4081 aactattact gataaatata acttcagtct ttacccaaat cctgctgctg agtcggtaaa
     4141 tattgttgga tcgtttccta aatttgttag agcgttcatt gttgaaccaa caggtaggat
     4201 tgttaatcag atcgaactaa atggaaattt taccaaagta gatatttctc aattgaaatc
     4261 aggtatttac cttattcaat tatttgacgg ggatcagcta ttattcacag aaaaaatgtt
     4321 agttccttaa cattacaaat taggtataac taaaaagagg ctgtctattt ttttagacag
     4381 cctctttttt tagaagatta actattcaat ttattattca ttacttgctt cagtttcttt
     4441 agttgtttct tcagcaaagt cttgtatttc ttcttcaatt attttatctt tttcggaata
     4501 gttatttaag atattaaggt ttgtaataaa taaatcagtg gctttttcta tttcatcttt
     4561 tttactttca atagcttgaa tatatttttc ttcgtagcca tt
//
LOCUS       DAOOAI010000125         4446 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_22047_length_4446_cov_4.558415, whole genome shotgun sequence.
ACCESSION   DAOOAI010000125 DAOOAI010000000
VERSION     DAOOAI010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4446)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4446
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22047_length_4446_cov_4.558415"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..177)
                     /locus_tag="PK345_07965"
     CDS             complement(<1..177)
                     /locus_tag="PK345_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019129899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HPX34988.1"
                     /translation="MSKLFIVPTPIGNLEDITLRALRILKEVDFILAEDTRNTAVLLK
                     HYNISKPLHAYHQNN"
     gene            270..1889
                     /locus_tag="PK345_07970"
     CDS             270..1889
                     /locus_tag="PK345_07970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HPX34989.1"
                     /translation="MSNFKHKITIYLPILFALVLIFGIIIGIGLVPRDTVKGNIFSVD
                     FSKYSKVGDVLRHIKRDWVDTVNLEKLEEDAIIEILENLDPHSQYISANEFDQANEYL
                     EGNFEGIGVQFRIEKDTAMVIIPIAGGPSDKVGVKAGDRIVIVDGDTVVGSEITSENI
                     MKRLKGKKNTKVNIQVFRRGESQLLEFNITRGVIPTYSLDIAYMIDANIGYIKLSKFS
                     STTSEEFSDALSKLLNEGMTKLILDLRNNAGGYLQAAIDVADEFLVDKQLIVYTEGKN
                     RATKYAFATKKGKFEQEPVIILIDEGSASASEIVAGAIQDNDRGIIIGRRSFGKGLVQ
                     EQITLFDGSAIRLTTARYYTPTGRSIQKPYGNGREEYFNEFHQRFVNGEVFHADSIHF
                     DDSLKFFTKKGKIVYGGGGIMPDIYIPIRNAKELVYYNRLIGRGLIYDFALDYTDANR
                     NSLNQYDNLRKFADNFKVSEAMFNDLISYADSQGLERDTKSINAMKEDIKALLKALIG
                     RNILDNEGFYPTFNEKDEILQEAIRQFKKII"
     gene            complement(1962..4376)
                     /locus_tag="PK345_07975"
     CDS             complement(1962..4376)
                     /locus_tag="PK345_07975"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HPX34990.1"
                     /translation="MQKSKIKRTKKKSADNQFQPIKIIYGIIILAYIFIPTFTPNWKS
                     LDTNTPKFFMTAVLNLAVFIFFLTSGYFKKNPAFFKGFLTDPIGIVYSALLGVTLLSF
                     TQSINILESVLQFSKVFTIFASAFNISMILMYDLRFLRLIIIVMAGLLAFDSISVFHN
                     INKFIEGEIKAITDIKTVYSNKNILASAIFVKLPFALYLFVFEKKWLKWISWLIFTLG
                     VVATFFMATRAFYLGLTVISIFFIIYHLVTYFHKKEREALNLTVAYLVSLLLAYGLFT
                     FTQENLYPQSKKSRHTSGVIDQMATLRNPKTASRLRVDAWRWSWELIKEKPLLGVGTG
                     NWKIQSLKLENQKNRGFIYLYKTHNDFIEITAETGIFGGLLFLSLFLLIAWNFLKRFT
                     YQKRIEDEGESKIDELGKYMFIAAVGMAFYSVDALFNFPADRPEITALFAFFVAAGVA
                     TSFQQKIDDHPTKETKNRLLSNKLFLNVIWIIAFIMLAVSTWILYINFQSTKTQRIVY
                     QEIIKGALKEPADNILTGFPFLPQVSVWGEPIETLKARYLINEERNEEAIEVLKNDRS
                     SPWDARRELFLATAYNNLKEYDSAIYYSEKMAELKPNYYRNVQLLASLYERKKKYGKV
                     IDLYNNFLDENKTEPQAWIHISSLYINKNDLEKAFELIKVGLKYNPRDSLIKKQHSFL
                     SHKLNTEPHLPIYNEATELFKNKEYQKTVEKLNEFLEKVPDYTSGYQIRAFCYYHLKD
                     TKKSIADADYALKLGSTNAGLINLRGVCKLDLKDTEGACKDFKRAMDMGDKSGESNWK
                     RFCQKK"
BASE COUNT         1411 a          732 c          839 g         1464 t
ORIGIN      
        1 attgttttga tgataagcat gcaggggttt tgaaatgtta taatgcttca ataacactgc
       61 cgtgttgcgg gtgtcttcag caagtataaa atcaacttct tttaaaattc gcaacgcccg
      121 taaagttata tcctcgagat tacctatagg agttggtaca atgaacagct tcgacataag
      181 gattgttgat atataagatt ataaaagaaa gtacgttaac aagtaaattt tatgttcctt
      241 tcttatttta atgttattca ccttttacga tgagcaattt taaacacaaa attacaattt
      301 atttacccat cctttttgcc ttggttttga tttttggaat aattatcggg attgggcttg
      361 tgccgcggga tacggttaaa ggtaatattt tctcagttga tttttctaaa tatagtaaag
      421 taggtgatgt tttgcgtcat ataaaacgcg attgggtgga taccgttaac ttagaaaaac
      481 ttgaggaaga tgctattata gagattttgg aaaatcttga cccccattcg caatacattt
      541 ctgccaatga atttgatcag gccaatgaat acttagaagg taactttgag ggaattggag
      601 ttcagtttag gattgaaaag gatactgcta tggtaataat tccaatagct ggaggaccct
      661 ccgacaaagt tggtgttaag gctggcgacc ggattgttat agtggatgga gatactgtag
      721 ttggttccga aattactagt gaaaacatca tgaaacgatt gaaaggcaag aaaaacacaa
      781 aggttaatat tcaagtcttt cgtagaggtg agtcccaact tcttgaattt aatattacac
      841 gtggcgttat tccaacctac agccttgata tcgcatatat gattgatgca aatattggct
      901 acattaagct gagcaaattc tcttcaacaa catcggagga attttcagat gctcttagta
      961 agcttctaaa tgaaggaatg acaaaattaa ttcttgattt acgtaataat gcaggtggat
     1021 atctacaagc tgcgattgat gttgcagatg aatttcttgt tgacaagcaa cttattgtat
     1081 atacagaagg gaaaaatagg gctactaaat acgctttcgc cactaaaaaa ggaaagttcg
     1141 agcaggagcc tgttattatc ttaattgatg aaggctcggc atcggcaagt gaaatagttg
     1201 ctggagccat acaggataat gatcgaggaa taatcatcgg ccggcgatcc tttggtaagg
     1261 gcttggttca ggaacaaatc acgctttttg atggttcggc cattaggctt actacggcca
     1321 gatactatac acccactgga aggtctattc aaaaacctta tggtaacggc agagaagaat
     1381 attttaacga gtttcatcag agatttgtga acggtgaagt ttttcatgct gatagtattc
     1441 attttgatga ttcgttgaaa ttttttacaa aaaagggtaa gatagtttat ggcggcggag
     1501 gaattatgcc agatatttat atcccgattc gaaatgctaa agagttggtt tattacaatc
     1561 ggttgatagg aagaggattg atttatgact ttgcattgga ttatacagat gctaatcgta
     1621 attcccttaa tcaatatgat aatttaagaa aatttgccga caacttcaag gtttcagagg
     1681 ccatgttcaa cgatcttatt agctatgccg atagccaagg tcttgaaaga gatactaaaa
     1741 gtattaatgc catgaaagag gatattaaag cgcttttaaa agccttaata ggtcgtaata
     1801 tattagacaa cgaaggtttt tatcctactt ttaatgaaaa ggatgagata ttacaggagg
     1861 caataagaca gtttaaaaag atcatttagc tttaatgaat cgagtttatt tggaagcgtt
     1921 gtttgtacta atattatctc aaatgtttaa atcaagattt gtcatttttt ttggcaaaaa
     1981 cgtttccagt tgctctcacc gcttttatcg cccatatcca tagctctttt aaaatcttta
     2041 catgccccct ctgtatcttt aaggtcaagt ttacaaacac ctctcaggtt tattaatcca
     2101 gcattggttg agcccaattt taaggcataa tcagcatcgg caatgctttt ttttgtgtct
     2161 ttcaaatgat aataacaaaa ggctcttatt tgataccccg aagtgtagtc tggcactttt
     2221 tcaagaaatt catttaattt ctcaaccgtc ttttgatatt ctttgttttt gaacagctca
     2281 gttgcctcat tataaattgg aaggtgaggt tcggtgttca atttatgtga aagaaaactg
     2341 tgttgttttt taataagact atccctcgga ttgtatttta aaccaacttt aatcagctca
     2401 aaggcttttt ccaaatcatt tttatttata tataagctgc taatatgaat ccatgcctgg
     2461 ggttctgttt tattctcatc taaaaaatta ttgtaaagat ctattacttt accatacttt
     2521 tttttccttt cgtaaagcga tgccaacaat tgaacattgc gatagtagtt tggtttaagt
     2581 tcagccattt tttccgaata atatatggcg ctgtcgtatt ctttcaggtt attgtaagca
     2641 gtggccaaaa acaactcacg ccgcgcatcc caaggtgatg aacgatcgtt ttttaacacc
     2701 tcgatagctt cttcgttcct ctcttcatta attaaataac gcgctttcaa cgtctctatg
     2761 ggttctcccc atacacttac ttggggtaaa aaaggaaacc ctgtaagtat attatcggct
     2821 ggttctttta aagcgccttt aataatttct tgataaacaa tcctttgggt ttttgttgat
     2881 tggaaattaa tgtacaaaat ccatgttgac acagcaagca tgatgaaggc tattatccag
     2941 atgacgttta ggaaaagttt gtttgataaa agcctatttt tagtttcctt cgtaggatga
     3001 tcatcaattt tttgttgaaa agaggtggca acaccggctg caacaaaaaa tgcaaataat
     3061 gctgtgattt cagggcgatc agcaggaaaa ttgaacaggg cgtcaacgct gtaaaaagcc
     3121 attcccactg ctgcgataaa catatatttt cctaactcat caattttact ttcaccttcg
     3181 tcttcaatgc gtttctggta ggtgaatctt tttaggaaat tccatgctat aagcaaaaat
     3241 aggcttaaga aaagtaatcc gccgaaaatg cctgtttctg cagtgatctc aataaagtcg
     3301 ttgtgtgttt tatagaggta tatgaaacct cgattttttt gattttcaag ctttaaactc
     3361 tgtattttcc agttgcccgt accaacgccg agcaatggtt tttctttaat cagctcccac
     3421 gaccatcgcc aagcgtcaac cctaagtcta ctcgctgttt ttgggtttcg caaggttgcc
     3481 atctggtcaa taacacctga tgtatgccgg cttttcttcg attgcggata tagattttcc
     3541 tgagtaaaag taaaaagacc gtaagccagt aatagagaaa caagatatgc aactgtcaga
     3601 tttagagcct ctctctcttt tttatgaaaa taagttacaa gatggtatat gataaagaaa
     3661 attgaaatga cagtaagacc aagataaaag gctcgtgtag ccatgaaaaa agtagctacc
     3721 acccctaagg taaatattag ccaactaatc catttcaacc attttttttc aaaaacaaac
     3781 agataaagag caaaaggcag ctttacaaaa attgctgaag caaggatgtt tttatttgaa
     3841 tagacagtct tgatgtcagt gattgctttg atttcgccct caataaactt attaatatta
     3901 tgaaaaacag aaattgaatc gaaagcaaga agccctgcca ttacgattat gatcagcctt
     3961 aaaaaacgaa gatcgtacat taaaatcatc gatatattaa aagctgaagc aaatattgta
     4021 aagacttttg aaaattgtag taccgattcc agaatgttta ttgattgggt gaaagaaagt
     4081 aaagtaacac caagcagggc tgaataaaca attccaatag ggtcagttaa aaagcccttg
     4141 aaaaatgccg gatttttttt gaaatagccg cttgtcaaaa aaaagataaa aactgccaaa
     4201 ttcagtacag cggtcataaa aaactttggt gtgtttgtat caagagattt ccaattaggt
     4261 gtaaatgtcg ggataaagat gtaggccaaa ataattatcc cataaatgat tttgataggt
     4321 tgaaattgat tatctgctga ttttttctta gtccttttga ttttagattt ttgcattgat
     4381 tttatttttg aacaatagac ttctgttttt atgtttcatc agaaaccgaa tcttgtttat
     4441 ctaaac
//
LOCUS       DAOOAI010000126         4402 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_22331_length_4402_cov_5.326892, whole genome shotgun sequence.
ACCESSION   DAOOAI010000126 DAOOAI010000000
VERSION     DAOOAI010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4402)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4402
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22331_length_4402_cov_5.326892"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..3559)
                     /locus_tag="PK345_07980"
     CDS             complement(<1..3559)
                     /locus_tag="PK345_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M14 family zinc carboxypeptidase"
                     /protein_id="HPX34991.1"
                     /translation="MKHFLLTLLISLFCISIFGQGWRKDEMEIKVNINTEEQASMLYR
                     LKLNGDIYPGYARLFVTPVEVERIKSLNFDYEVLIPDLNKHYENFWETRDAYHSYDEI
                     IELADSLVEHFPDICKKIIYGTSLGGRQLACLKISSNVNVNEIKPQVGFDGGIHGDEI
                     GCSENVIRFARDLCLNYGNDPTITNLINTREIWLYLMVNPDGRVNMVRYNNNGVDLNR
                     DWGYMWENEGGSTGAFSQIESKVLRDFVFENNFVVHTSYHSGTEYISCPWSYRASQPP
                     DYNHILQLAGVYANVSGYSNMPYGQGYSGMYPINGSTKDSNYGLKGSVSWSLEISMSK
                     QPPASQIMMYYNYNRPGMIAMIEYAGYGIRGIVTDAVTGEPVTASVFVNNFLPCFTDP
                     ENGDFHKYLTPGTYSVKVKANGYATTTVNNVVVTANNATEVNITLQPEDHKSIYRVIS
                     CHIPNNNPADPGAAWNVIGPPDNQYYSLGKNGWIVVDMQEMIYDGAGPDIIVFEGDNT
                     PEGYTLYAGSTMDGPWHSMGTGSGTTEFDFANCTLSEARYFRVLDDGDGSANVNGAGF
                     DLDAIQVLSSITGPYIIMSDYVVDDSTGNNNGQLDPGETATFVITLKNVGTETALDII
                     GSLTSSDEYITVLTTSPQVFGNIEINNTATAQYTVSASSSAPAGHTTNLNLEYAGTNL
                     NSQVKTIPVLFPDYCYPTASCSFGDGFTGFSLEQINNMNSGCSNDNGISGYGDFTSMT
                     TELQPGNTYTVSWKSGYSNQKASLWIDLDNDMSFSDNERLITDFSLSQSNTLYTTNFT
                     LPESVLPGEKRLRIRANWQSSSSNPCSNFTYGETEDYTVIIPGNTLNAAFIPSVTEIC
                     HGDQVQFTDQSTGNITGWQWTFEGGTPSTSNLQNPLVTYQTPGDFDVSLTVTDGSNSN
                     TYSQLNLIHVFDDSPVPSMPSGPSNLCQANEPTSYTSSAPNATSWLWKLEPAQAGTIS
                     FQGAAATVNWNESFSGSVLLSAATSNLCGESQYSNPLVIEIMPFPADASAIEGEDKVC
                     QGGIDSYETNEIEAATSYEWILTPAQAGNLTSTENQCQIEWSKTYEGTASLKVRGSNE
                     CGFGNWSPLFDILVENCSGINKVVSNKIIIYPNPANGLFYVKTGFPDNIKVRITITNI
                     SGIKIYEHEGMDNDFTIDLKNVSNGIYYIKFETEEHTLVK"
     gene            3831..>4402
                     /locus_tag="PK345_07985"
     CDS             3831..>4402
                     /locus_tag="PK345_07985"
                     /inference="COORDINATES: protein motif:HMM:NF025647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4369 domain-containing protein"
                     /protein_id="HPX34992.1"
                     /translation="MKKKILAVCSLMLIIGFALISCTSNDDRRQSTIKGRFGAMAGQK
                     IVLQQLTINNMIKVDSVIASTDGSFSFDFQAEETGFYMLHFTDGSFITLIINSDDKLE
                     ITSDAAPGSMQYQISGNEDSQLLQNYFVKTAERETVFDSLRKVFFNSTHLENFYAVKL
                     NVDSVLRQLVEDQYSFTKNLILNNSGSFASL"
BASE COUNT         1228 a          901 c          888 g         1385 t
ORIGIN      
        1 ttttaaccaa ggtatgttct tcggtttcaa attttatgta ataaattcca ttggatacat
       61 ttttaagatc aatggtaaag tcattatcca ttccttcatg ttcataaatc ttgataccag
      121 aaatatttgt tatggttatt cttactttaa tgttgtcagg aaaacctgtt tttacataaa
      181 ataagccatt tgcgggatta ggataaatga taattttatt ggaaacaact ttgttaattc
      241 cagagcaatt ttcgacaagg atatcaaata atggcgacca gttgccaaag ccacattcgt
      301 ttgatcctct tactttaagc gatgctgtcc cttcgtaggt tttgctccat tctatttggc
      361 attgattctc agttgaagtc agattgccgg cttgtgcagg cgtaaggatc cattcgtaac
      421 tcgtagctgc ttctatttcg tttgtttcat aagaatctat acccccctga caaaccttat
      481 cttcgccttc aattgctgag gcatcagccg gaaatggcat tatctctata actaagggat
      541 ttgaatattg actctctcca cacaaattgc tagttgctgc agaaagtaga actgagcctg
      601 aaaagctttc attccagttt actgtggcgg ctgcgccttg aaagcttatc gtgccggctt
      661 gtgcaggttc cagtttccat aaccaggatg tggcattggg agctgatgag gtgtatgaag
      721 ttggctcatt agcctgacac aggttcgaag gacctgatgg cattgaagga actggagagt
      781 cgtcaaaaac atgtattaag ttcaactggc tgtaagtgtt gctattcgaa ccgtcggtaa
      841 cggtaaggct aacgtcaaag tctcccggtg tttgatatgt aactaacggg ttttgcaaat
      901 tggaagtaga gggtgtccct ccctcaaaag tccattgcca acctgtaata tttccggttg
      961 attgatccgt aaactgaacc tgatcgccat ggcagatctc cgtaacactt gggataaaag
     1021 cagcatttag agtgttgccg gggatgatca cggtatagtc ctctgtttcg ccataagtga
     1081 agtttgagca tggattggag gaggagcttt gccaattagc ccgtattctc aaacgttttt
     1141 cgcccggtag aacagattcc ggcaaggtga aatttgtagt ataaagggtg ttggattgcg
     1201 ataagctgaa gtcagtgatc aggcgttcat tatcactgaa actcatatcg ttgtccaagt
     1261 ctatccataa gcttgccttc tggttgctgt atccgctctt ccacgatacc gtataagtat
     1321 ttcccggttg aagttctgta gtcatcgagg taaaatcacc atatccacta atgccgttat
     1381 cattcgaaca gccgctattc atattgttga tctgctctaa agagaatccc gtaaatccat
     1441 cgccaaatga gcaacttgcc gtaggataac aataatcagg gaataggaca ggtattgtct
     1501 ttacctgtga gtttaaattg gtgcccgcat attccaaatt aaggtttgta gtgtggccgg
     1561 ctggcgctga actgcttgca ctcacggtat attgagccgt ggccgtattg ttgatctcaa
     1621 tattaccgaa aacttgtggt gaagtggtca gaacagtgat gtattcatcc gaactggtca
     1681 atgaaccgat gatgtcaagt gcagtttcag ttcctacatt tttcagggta attacgaacg
     1741 ttgccgtttc gcccggatcg agttgaccgt tattattacc tgtcgaatca tcaacaacat
     1801 aatcactcat aattatataa ggccctgtaa tagagcttaa aacttgtatt gcatcaaggt
     1861 caaaaccggc tccgttaaca ttagctgatc catcgccatc atccaacact ctgaaataac
     1921 gagcttccga caaggtgcaa tttgcaaaat cgaactccgt tgtaccgctc cctgtaccca
     1981 ttgaatgcca cgggccatcc attgtggagc cggcataaag agtatagccc tcgggtgtgt
     2041 tatccccctc aaaaacaata atatccggac ctgccccatc ataaatcatc tcttgcatat
     2101 ccactacgat ccatccgttt tttcctaacg aatagtattg attgtctggc ggaccaataa
     2161 cattccaagc agcgccaggg tcggcaggat tattattagg gatatggcag cttatcactc
     2221 tgtaaatact tttatggtct tcgggttgaa gcgtaatatt gacctctgtg gcattatttg
     2281 ccgttactac aacattattt acagtagttg ttgcataacc attagctttc accttaacgc
     2341 tatatgtgcc tggagtcaga tatttatgaa aatcaccatt ttccggatca gtaaagcagg
     2401 gaagaaagtt atttacaaac actgaagctg ttactggttc gcctgttact gcatcggtta
     2461 ctatccctct tataccgtac ccggcatact caatcatggc aatcattccc gggcggttgt
     2521 agttgtaata catcattatt tgtgatgcgg gcggctgctt gctcatcgag atctccaatg
     2581 accagctaac agacccttta agtccatagt ttgagtcttt agtactgcca ttgatcgggt
     2641 acattcccga atagccctga ccgtaaggca tattgctgta ccccgaaaca ttggcataaa
     2701 caccggcaag ctgtaaaatg tggttatagt cagggggttg agatgctctg tagctccatg
     2761 ggcacgagat gtattcggtg ccactgtgat aggaggtgtg tacgacaaag ttattttcaa
     2821 aaacaaaatc tctcagaact ttcgattcaa tttgtgaaaa ggctcctgta ctaccgccct
     2881 cattttccca catatagccc cagtcgcgat taaggtcaac gccattattg ttatatcgta
     2941 ccatattgac gcgcccatcg ggattaacca tcaggtaaag ccaaatctca cgggtattaa
     3001 taaggtttgt tattgtagga tcgtttccat aattcaagca aagatcacga gcaaaacgaa
     3061 tgacattttc tgagcagcct atctcgtcgc catgaatacc tccatcaaaa ccgacttgtg
     3121 gctttatctc attcacattt acattactgc tgattttaag gcaagccaat tgacgaccgc
     3181 ccaatgaggt gccgtaaatg atttttttac atatatccgg aaagtgttct accaagctgt
     3241 cggcaagctc gatgatttca tcgtaggagt gataggcatc acgtgtttcc caaaagttct
     3301 cataatgttt gttaaggtcg gggatgagaa cttcatagtc gaaattcaga cttttaatcc
     3361 tttcgacctc aaccggcgtt acaaatagcc tcgcatagcc cggataaata tctccgttaa
     3421 gtttcaaccg gtaaagcata ctagcctgtt cttcagtatt aatgtttact tttatctcca
     3481 tttcatcttt tcgccaaccc tggccgaaaa ttgaaataca gaacaacgaa atgagcaaag
     3541 tgagaagaaa atgtttcatg aggtgaagtt ttatagttaa tacaattctt ttgtattgtg
     3601 ctgataatcc atatgttaga ttcttttatt cagctttaat ggttaattat ttaaatggct
     3661 aaaataatga atattaaaaa gctgatattt ttaaagtatc gaactacctt tttattctaa
     3721 tttttttgtg aaacgaactc aaaaattgcc tcttagattt ttttttaaaa ggtatcaatt
     3781 atcctaataa tttcgttcgt ttgcaataaa gcattaattt cagttttttt atgaaaaaaa
     3841 agattttagc agtctgttct ctaatgctga tcatagggtt tgcgttgatt agctgtacct
     3901 caaatgacga tcggcggcag tcaactatca aagggagatt tggcgcaatg gcgggacaaa
     3961 agatcgtcct tcaacagttg actattaaca atatgatcaa agtagattct gtaatcgctt
     4021 caacggatgg cagttttagt tttgattttc aggccgaaga aacgggtttt tatatgttgc
     4081 actttactga tggatcgttt attacactta ttatcaattc tgacgacaaa cttgagataa
     4141 cctcggatgc tgctcctggt tcaatgcaat atcagatttc gggaaatgag gattcccaac
     4201 tgcttcagaa ttattttgta aaaactgctg agagagaaac tgttttcgat tcattacgta
     4261 aggttttttt taacagtaca catctcgaga acttttatgc cgtcaaactg aatgttgata
     4321 gcgttttgag gcaactggtt gaagatcagt actcttttac aaaaaatctt atccttaata
     4381 attcaggctc gtttgcatcc ct
//
LOCUS       DAOOAI010000127         4376 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_22515_length_4376_cov_4.956954, whole genome shotgun sequence.
ACCESSION   DAOOAI010000127 DAOOAI010000000
VERSION     DAOOAI010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4376)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4376)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4376
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22515_length_4376_cov_4.956954"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..180)
                     /locus_tag="PK345_07990"
     CDS             complement(<1..180)
                     /locus_tag="PK345_07990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009137163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HPX34993.1"
                     /translation="MDSEKRYNLRGVSASKDDVHKAIRNIDKGLFPKAFCKIIPDFVG
                     QHPDYCNIMHADGAGT"
     gene            386..467
                     /locus_tag="PK345_07995"
     tRNA            386..467
                     /locus_tag="PK345_07995"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:420..422,aa:Leu,seq:tag)
     gene            complement(527..901)
                     /gene="trxA"
                     /locus_tag="PK345_08000"
     CDS             complement(527..901)
                     /gene="trxA"
                     /locus_tag="PK345_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005643748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HPX34994.1"
                     /translation="MEHLTKETFLTKVFNYEKNKEWKFEGSLPCLIDFYADWCAPCRM
                     VAPVLEELAKEYAGKINIYKIDTEDQQELAAIFGIRSIPSLLFCPLNEPPQMAQGALP
                     KNTLKQVIDDVLLKKETLEAKK"
     gene            complement(936..1418)
                     /locus_tag="PK345_08005"
     CDS             complement(936..1418)
                     /locus_tag="PK345_08005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005942325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HPX34995.1"
                     /translation="MNKLFLSVFPVILLAFTVSGCNSTSGNEGSNLTGDEKSSAKIEF
                     LTKETFKQKVWDYEKNQQTWVYEGKLPAIIDFYADWCRPCRMVAPILDELAEDYKGKV
                     VIYKINTQVEKELAAIFKIQSIPSFLYIPVQGQPQMDKGLKDKAAFEKIIAEVLLKNN
                     "
     gene            1594..2004
                     /locus_tag="PK345_08010"
     CDS             1594..2004
                     /locus_tag="PK345_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HPX34996.1"
                     /translation="MSNIFSQIGNIDLESAFQNADQCLELLAAEKWKDGYICRKCGNT
                     NYCFGKTLHSRRCTRCKHDESATANTIFHRCKIPLNEAFKIAYIVCNQPDISTYDISR
                     MLKIRQMTCWKFKKRISECLDKKGYFDVMEMPNS"
     gene            2165..3553
                     /locus_tag="PK345_08015"
     CDS             2165..3553
                     /locus_tag="PK345_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016269978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HPX34997.1"
                     /translation="MFKPEVYKQRRTALYSQNLKGIALFFGNEESPMNYAANTYAYRQ
                     DSTFLYFFGLDMPGLAGVIDFDEEKEIIFGNDVDMEDIIWMGPQPKMTDNALKAGITH
                     VEPFKNLEQFLKSALQKGREIHFLPQYRHDNKILLESLLGIAISKQKERASVALIKAV
                     VNLRSVKDKYEIEELEKAAAIGYQMHVTSMKMAKPGIYEREIAGAIEGIALSQGAGVS
                     FPIILSQNGQTLHNHDHSQILEKGRLMVTDAGAETVLRYSSDFTRTVPVGGKFSQRQR
                     DIYQIVLDANNAATAAVKPGITYKSVHLLVSKVIASGLKNLGLMKGDVEEAVRNGAHA
                     MFFPHGLGHMMGLDVHDMEDLGEDYVGYDEEVKRSDQFGIAYLRLGRRLKTGFVLTNE
                     PGIYFIPELISLWKSENKFTDFINYAKVEEYIGFGGIRLEDDLLVTEHGCKLIGKRVP
                     ITIDEVEETMRD"
     gene            complement(3607..>4376)
                     /locus_tag="PK345_08020"
     CDS             complement(3607..>4376)
                     /locus_tag="PK345_08020"
                     /inference="COORDINATES: protein motif:HMM:NF040069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6340 family protein"
                     /protein_id="HPX34998.1"
                     /translation="IETLADMISYNPRFTTTTIESLEIALPVLPEEITNRDVNYLKQL
                     CEEHQADAIVILAKTEKQVGYSKYYGHFGGYFSMITIIYNTRWMFINPFAPIMIDHKN
                     IRDTLYYRVGGIYGTLDPEFYQFVGELMMETTDYVASDYGMRITPHFAETQRIVFNKG
                     DKYLKKGFSEAESGNWVKASEIWQKTLSYNNPVIVSRGAFNIALSNEMEGNLKLAADW
                     AEVSYDIFPDIINKTYLDILQERIRQQDDLMKQMDGP"
BASE COUNT         1347 a          818 c          797 g         1414 t
ORIGIN      
        1 tgtaccagct ccatcagcgt gcataatgtt acaataatca ggatgctgac ctacaaaatc
       61 aggtataatt ttacaaaatg cctttgggaa caaaccttta tcaatgttcc taatagcctt
      121 atgaacatca tcttttgagg cggatacacc acgtaagttg taacgtttct cgctatccat
      181 atttttttct gcaaacttat ctaattaact tcaaatcgtt agtaatttgc agttaattaa
      241 acaaagttga ttgattatca ctatggtttt attactcgaa acttgctttt ttaaagctta
      301 acacgctaat ttttagtatt tttattcttt tttaataatt ctttttttta ctttatttta
      361 aaaatatatt tttgtggttt caaatgcgga tgtggcggaa ttggtagacg ctccagactt
      421 agaatctggt gccgcgaggc gtgggggttc gagtcccttc atccgcacta aaaagaaaaa
      481 ggctgatcgc ttcaggtcag cctttttttt aaatagatgc aattttttac ttctttgctt
      541 ctaaggtctc tttctttagt aatacatcat ctattacctg tttcaatgta ttttttggca
      601 aggcgccctg tgccatctgc gggggttcat tcaaggggca aaatagcaac gaagggatac
      661 tgcgaattcc aaatatagca gccaactctt gctgatcctc agtatcaatt ttataaatat
      721 taatcttacc tgcatactct tttgcaagct cttccaacac aggagcaacc atacgacatg
      781 gagcacacca gtcagcataa aaatcaatca aacatggcaa actgccttca aatttccact
      841 ccttgttttt ttcgtaatta aaaactttgg taaggaatgt ctctttagtt aaatgttcca
      901 ttttatagtt tttatattga ttatcaaaat tttaattaat tattttttaa aagtacctcg
      961 gcgattattt tttcgaaagc agctttatct ttcagccctt tatccatttg aggctgccct
     1021 tgcacaggta tataaagaaa agagggaatg ctctgaatct tgaaaatagc tgcaagctct
     1081 ttctcaactt gagtattaat tttatagatt accactttac ctttataatc ttcggccaac
     1141 tcgtccaaga taggcgctac cattcggcaa ggacgacacc agtcggcata aaaatcaata
     1201 atagcaggaa gtttgccttc atacacccag gtttgttgat tcttctcgta atcccatact
     1261 ttttgtttaa acgtctcttt ggttaaaaac tcaatcttgg ctgatgattt ttcatctccg
     1321 gtaagattac tgccttcatt acccgaagta gagttacacc ctgaaacagt aaaggccaat
     1381 aaaataacag gaaacaccga taaaaataat ttattcatag tctgttaact atttagtggt
     1441 taatatttca tttaaaaatc gctgcaaaag taattctttt ttattttaaa tcgctttagt
     1501 atcacttttt aactattttt gcaagaaaaa gttcattatg gttttttgat tttactattt
     1561 aaaaccataa atgattaatt atcaacaaag cgcatgagta atattttttc gcaaattggt
     1621 aatattgatt tagagtccgc ttttcaaaat gctgaccaat gtctcgaatt attggctgcc
     1681 gaaaagtgga aagatggcta tatctgccga aaatgcggca acacaaatta ttgttttgga
     1741 aaaacgctcc attcacggcg ttgtacacgc tgcaaacatg atgaatcggc tactgcgaac
     1801 accatctttc atcgttgtaa aattccgttg aacgaagctt tcaaaatagc ttatatcgtc
     1861 tgtaatcaac ctgacatatc cacttatgat atttcacgta tgcttaaaat ccgccaaatg
     1921 acatgctgga aatttaaaaa gagaatatcg gagtgtcttg ataagaaagg atacttcgat
     1981 gtgatggaaa tgcctaactc ataaagtttt gagaaacttg atcgctttgc taaaactttc
     2041 tcaaattaat tttcatgaaa ctcaaaatcc caaatacctc ttttaaaaat aaccacctac
     2101 cctataatgc ttatttttgc aataatttac ttttttcact attactcaaa taatttttct
     2161 acaaatgttt aaaccagaag tttacaaaca gagacgtacc gctctttata gccaaaacct
     2221 caaaggtatt gctctttttt tcggcaacga agaatcaccg atgaattatg ctgcgaatac
     2281 ttatgcttac cggcaggaca gcacattttt atatttcttc ggcctcgata tgccaggttt
     2341 agcaggcgtc atagattttg acgaagaaaa agagatcatt tttggaaatg atgtagatat
     2401 ggaggatatc atctggatgg ggccacagcc caaaatgacc gataacgctc ttaaggcagg
     2461 aataacccat gttgagcctt ttaaaaatct agaacagttt ttaaaaagcg ctttacaaaa
     2521 aggcagggag atccatttcc taccgcaata tcgtcatgac aataagattt tactcgaatc
     2581 tctcttaggc atcgctattt cgaaacagaa agagcgtgca tcagtagcat tgatcaaagc
     2641 tgttgtgaat ttgcgtagcg taaaggataa gtatgaaatt gaggagttgg agaaggctgc
     2701 agctataggc tatcagatgc atgtaacctc catgaaaatg gctaagcctg ggatttatga
     2761 gcgtgagatt gccggcgcta tcgagggcat tgcactttca caaggggctg gtgtctcttt
     2821 cccaatcatc ttatcacaaa atggacagac gttacacaac catgatcata gtcagatttt
     2881 agaaaagggc agattgatgg taactgatgc aggggctgaa actgtattgc gctattcgag
     2941 tgattttaca cgaactgtgc ctgttggtgg aaaattttcg cagcgtcagc gtgatattta
     3001 tcagatagtg cttgatgcca ataatgctgc tacagcagcg gttaaaccag gaatcactta
     3061 caagagtgta catttgcttg tttccaaagt gatcgcatca ggtttaaaaa accttgggct
     3121 aatgaaaggt gatgtggaag aagctgtacg caatggagct catgcaatgt tttttccaca
     3181 tggtctgggg cacatgatgg ggcttgatgt tcacgacatg gaagatcttg gcgaagacta
     3241 tgtcggctat gacgaagaag ttaaacgtag cgatcaattt ggtatagctt atctgaggct
     3301 tggacgtagg cttaaaaccg gttttgtgct aaccaacgag ccaggcattt attttatccc
     3361 cgaactaata agcctttgga aaagcgaaaa taaatttacc gattttataa attatgctaa
     3421 agttgaggaa tacattggct ttggaggtat aagattagag gatgacttac ttgtaaccga
     3481 acatggttgt aaactgattg gaaaacgcgt tccaattaca attgatgaag tggaagagac
     3541 aatgagggat tagtactttg ttataaatca ataaagtgta tcaataatta aaaattgcct
     3601 ttcaagttaa ggtccatcca tctgtttcat caaatcatcc tgctgcctaa ttcgttcctg
     3661 aaggatatct aaataggttt tgttgattat gtcagggaat atgtcgtacg acacttctgc
     3721 ccaatcggct gcaagtttta agttgccttc catttcattg gaaagtgcaa tattaaaagc
     3781 tcctcgagag acaattaccg gattattgta actaagggtt ttctgccata tttctgatgc
     3841 tttaacccaa ttcccacttt cagcctcgct aaatcctttt ttcagatatt tatctccctt
     3901 attaaataca atgcgttgcg tctctgcaaa atgcggggtt atcctcatgc cataatcaga
     3961 agctacataa tcagttgttt ccatcatcaa ctctccaaca aattgataaa actcagggtc
     4021 tagtgttccg taaattccac cgacacggta atagagagtg tctctaatat ttttatgatc
     4081 aatcattatg ggtgcaaaag gatttataaa catccagcgt gtattataga taatggtaat
     4141 catgctaaaa taccctccaa aatgaccgta atattttgaa tatccaacct gtttttcagt
     4201 cttagcaaga attacaatag catcagcctg atgctcttcg cataattgct tcaagtaatt
     4261 aacatctcta ttggtaatct cttcggggag cacaggtaaa gcaatctcca aactttcaat
     4321 agtggtagtg gtgaatcttg ggttatacga aatcatatcg gcaagagtct cgattg
//
LOCUS       DAOOAI010000128         4374 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_22535_length_4374_cov_5.145636, whole genome shotgun sequence.
ACCESSION   DAOOAI010000128 DAOOAI010000000
VERSION     DAOOAI010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4374)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4374
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22535_length_4374_cov_5.145636"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(63..773)
                     /gene="rplQ"
                     /locus_tag="PK345_08025"
     CDS             complement(63..773)
                     /gene="rplQ"
                     /locus_tag="PK345_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004350214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HPX34999.1"
                     /translation="MRHRKIYNQLSRTSSHRNAMLSNMAVSLILHKRINTTVTKAKAL
                     RAYVEPLLTKSKEDTTHSRRVVFSYLQNKEAVTELFREISPKIADRPGGYTRIIKTDF
                     RLGDNADMCMMELVDFNDVYLAKESLSADKGGRRRRRGSGKTRKDQAAAPAVEETTKT
                     EVEQPEMIVEQQVDETLEEIQTPEPRVDDKIVEEQVDETPDETQTSKLPVDDKIAESQ
                     DSQKDTEDTESPSKEKEA"
     gene            complement(781..1779)
                     /locus_tag="PK345_08030"
     CDS             complement(781..1779)
                     /locus_tag="PK345_08030"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018109216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HPX35000.1"
                     /translation="MAILAFQKPEKVIMLESSNYKGKFEFRPLEPGFGITIGNALRRI
                     LLSSLEGFAITTIKIEGVDQEFSTIKGVVEDVVEIVLNLKQIRFKRLIEQEDSESVKI
                     VIKDQEVFKAGDLNKYLSVFQVLNPDLVLCNMDPSVTLTMDMTINKGRGYVPAEENKL
                     LNSPLPLGTIAIDSIHTPIVNVKYNVEDYRVEQKTDYEKLLLEIITDGSIHPKDALKE
                     AARILIIHFMLFSDERIAMEKEDKSVTEEFDETALSIRQVLKTKLVDLNLSVRALNCL
                     KAADIETLGELVTFNKNDLLKFRNFGKKSLAEIEELFESKNLEFGMNVAKYKLDKK"
     gene            complement(1816..2421)
                     /gene="rpsD"
                     /locus_tag="PK345_08035"
     CDS             complement(1816..2421)
                     /gene="rpsD"
                     /locus_tag="PK345_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HPX35001.1"
                     /translation="MARYIGPKTKISRKFGEPIYGPDKSFEKKNYPPGQHGLNKKRKK
                     PTEYGIQLAEKQKAKYTYGILERQFRNTFEKASRKQGVTGEVLLQFIETRLDNVVYRL
                     GIAPTRAAARQLVSHCHIVVNGEVVNVPSFHVKPGFSVGVRERSKSLEVINNTIASRH
                     SLLPWLEWDEEKMTGKVLNYPQRDEIPENINEQIIVELYSK"
     gene            complement(2456..2851)
                     /gene="rpsK"
                     /locus_tag="PK345_08040"
     CDS             complement(2456..2851)
                     /gene="rpsK"
                     /locus_tag="PK345_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004331557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HPX35002.1"
                     /translation="MAKSKTSKTTKKRVVKVDPIGKAYISASFNNILVTLANNEGQVI
                     SWSSAGKMGFRGSKKNTPYAAQTAAEDCSKTAYDLGLRKVKVYVKGPGSGRESAIRTI
                     HSSGIEVLEINDITPLPHNGCRPPKRRRV"
     gene            complement(2913..3293)
                     /gene="rpsM"
                     /locus_tag="PK345_08045"
     CDS             complement(2913..3293)
                     /gene="rpsM"
                     /locus_tag="PK345_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009125149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HPX35003.1"
                     /translation="MARIAGIDLPKNKRGVVGLTYIHGIGPNLAQEILDRVGVDVNKK
                     VQDWSDEEQTNIRALISDEYKVEGALRSETQMNIKRLMDIGSYRGIRHRIGLPVRGQS
                     TKNNARTRKGRKKTVANKKKAPKG"
     gene            complement(3320..3436)
                     /gene="ykgO"
                     /locus_tag="PK345_08050"
     CDS             complement(3320..3436)
                     /gene="ykgO"
                     /locus_tag="PK345_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014790280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HPX35004.1"
                     /translation="MKVRASVKTRTPDCKIVRRKGRLYVINKKNPKYKQRQG"
     gene            complement(3446..3664)
                     /gene="infA"
                     /locus_tag="PK345_08055"
     CDS             complement(3446..3664)
                     /gene="infA"
                     /locus_tag="PK345_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017185218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HPX35005.1"
                     /translation="MAKQPLIEQDGTVIESLGNAMFRVELENGHVIIAHISGKMRMHY
                     IKILPGDRVQVAMSPYDLSKGRITFRYK"
     gene            complement(3670..>4374)
                     /gene="map"
                     /locus_tag="PK345_08060"
     CDS             complement(3670..>4374)
                     /gene="map"
                     /locus_tag="PK345_08060"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HPX35006.1"
                     /translation="IKPGVTTNKLDKVAEEFIRDHDAIPGFKGYHGFPGTLCTSVNEV
                     VVHGIPSDYVLKDGDIVSIDCGVKKNGYFGDSAYTFPVGEVKKEILMLMKRTLESLYK
                     AIDVAIGGNRIGDIGFAIQEYVEGFGYSVVRDLVGHGLGKNLHEKPEVPNYGKRGYGK
                     ELRAGMVLAIEPMINLGVKDVYQKSDGWTIKTKDHLPSAHYEHNVVVRKDKADVLSTF
                     DFIEEVTNFSILNITI"
BASE COUNT         1128 a          937 c          733 g         1576 t
ORIGIN      
        1 taagatagac aaagcttagt ttttaagctt tatccatttc ggttatcaaa aaaataagga
       61 ttttatgctt ctttttcttt gctcggtgat tcagtgtctt cagtgtcttt ttgcgagtcc
      121 tgactttcag cgattttatc gtcaacaggc agcttggagg tttgtgtttc atccggtgtc
      181 tcatcaacct gctcttcaac aattttgtcg tcaacacgcg gttctggggt ttgtatttct
      241 tccagtgtct catcaacttg ctgttcaaca atcatctctg gctgttcgac ttcggttttt
      301 gtagtctctt ccacagccgg tgcggcggct tgatcctttc ttgttttgcc cgagcctctt
      361 cttcttcggc ggcctccctt atctgctgat aagctttctt tagctaaata tacatcgttg
      421 aaatcaacca gttccatcat acacatatcg gcattatcgc caagtctgaa atcagttttg
      481 ataatacgtg tatatccgcc tgggcggtcg gcaatttttg gagatatctc tctaaagagt
      541 tcagtaactg cctctttatt ttgcaaataa ctaaaaacaa ctcttcttga gtgtgtggta
      601 tcctcttttg attttgtaag cagaggttct acgtaagctc taagcgcttt tgcttttgta
      661 acagtagtat ttattcgctt atgcaatata agggacacag ccatattaga aagcatcgca
      721 ttacgatggc tgcttgttct gcttaattga ttataaattt tacgatgtct cattttacga
      781 ttacttcttg tctaatttat atttcgctac attcattcca aattcaagat ttttagattc
      841 aaaaagctcc tctatttcag cgagagattt ctttccaaaa ttcctgaatt ttagcaggtc
      901 gtttttatta aaagttacca attcaccaag tgtttcaata tcagcagctt tcaagcagtt
      961 taaagctctg acggaaagat tcaggtcaac tagtttagtt ttaagtactt gtcgaatact
     1021 tagcgcagtt tcatcaaatt cttcagtcac ggatttatct tctttttcca tggcaattct
     1081 ttcgtctgag aaaagcataa agtggattat aagtatccgt gcagcttctt ttaatgcatc
     1141 tttcggatgg attgacccat cagtaataat ttctaatagg agtttttcat agtcggtttt
     1201 ttgttcaact cgatagtcct ccacattata ttttacattt acaattgggg tatgtattga
     1261 gtctatggca attgtaccta atggtaatgg cgaatttaat agtttatttt cttccgccgg
     1321 cacataccct cttcctttat ttatggtcat atccatcgtc aaagtaaccg atggatccat
     1381 attgcatagt accaaatcag gattcaacac ttgaaacaca gatagatatt tatttaaatc
     1441 acctgcttta aatacttcct gatctttgat aactattttt acactttctg agtcttcttg
     1501 ttcaataagc cgtttaaagc gtatttgttt taggttaagt actatttcta ccacatcttc
     1561 taccacgcct tttatggtag aaaactcctg atccactcct tcaattttaa tagtggttat
     1621 tgcaaatcct tcaagcgagg ataataaaat cctgcgtaaa gcattgccaa tagtaattcc
     1681 aaatccgggt tccaagggac gaaattcaaa cttacctttg taattgctcg attcgagcat
     1741 gattactttt tcgggctttt gaaatgctaa tattgccata ataaaacctt ttaggttgca
     1801 aataattaaa agatactatt tagaatagag ctcaacaatg atctgctcgt ttatattttc
     1861 aggaatctca tctctttgcg ggtagttaag cactttcccc gtcatttttt cttcatccca
     1921 ctcaagccat ggtaacaatg agtggcgtga agcaattgta ttgtttatta cttctaatga
     1981 ttttgatctt tccctaacac caacgctaaa tccgggtttc acatgaaatg acggcacatt
     2041 taccacttca ccgtttacca caatatggca atgtgaaacc aactggcgag cggcagcgcg
     2101 tgtaggagct atgccaagcc ggtaaacaac attgtcgagg cgggtttcga taaactgaag
     2161 taacacctca ccagtaactc cctgctttcg gcttgccttt tcgaaagtgt tgcgaaactg
     2221 gcgctcgagt atcccataag tatatttagc tttttgtttt tcggccaact gaataccata
     2281 ttccgttggt tttttacgtt ttttatttaa tccatgctgt cctggaggat aatttttctt
     2341 ttcaaaagat ttatccggtc catagatcgg ttctccgaat tttcttgaaa tcttggtttt
     2401 aggcccaata tatcttgcca ttttatctca aattttattt ttattcaatg atttattata
     2461 ctcttcttcg ttttggcggt cggcatccgt tatgaggtaa tggggtgatg tcatttattt
     2521 caagtacctc aataccggac gaatgaattg tacggattgc tgattctctt ccggagccgg
     2581 gtccttttac ataaactttt actttgcgca aaccaaggtc ataagctgtc tttgagcaat
     2641 cttcggctgc tgtttgtgca gcataagggg tatttttttt cgatcctctg aatcccattt
     2701 ttcctgctga tgaccatgag ataacctgcc cttcattatt ggcaagagtt actaaaatat
     2761 tgttaaaaga agcgctgata taggctttcc cgataggatc aaccttaaca accctctttt
     2821 ttgtggtttt acttgttttt gattttgcca tattattgat ttcttgttga cttaattaat
     2881 taaactacca ataaaatgtt ggtatttcaa ttttaacctt tcggtgcttt ctttttatta
     2941 gcaacggtct ttttgcggcc tttacgtgtt cgggcattat tttttgttga ttgccctcta
     3001 accggcaaac cgatacggtg acgtattcct cgataactac cgatatccat caaacgtttg
     3061 atgttcatct gtgtctcgga gcgaagcgca ccttccactt tgtattcatc gctgatcagc
     3121 gcacggatgt tagtttgctc ttcgtccgac caatcctgca ctttcttatt tacatccaca
     3181 ccaactctat cgagtatctc ctgtgcaagg ttaggaccga taccatgaat atatgtcaga
     3241 ccaactacac cgcgtttatt ctttggtaag tcaatacctg caatacgtgc catagtaatt
     3301 tatctcttta attaatttat taaccttgtc tttgtttgta ctttggattc tttttattaa
     3361 tcacgtacaa ccttcctttt cgacggacaa tcttgcagtc cggcgttctt gttttcactg
     3421 atgctcttac tttcatcgct atcaattatt tatatctgaa tgtaatccgt ccttttgata
     3481 agtcataagg cgacatagca acctgaacgc ggtcgccagg aagaattttt atgtaatgca
     3541 tcctcatctt gcccgatatg tgcgcaatga tgacatgtcc attttctaat tcgaccctga
     3601 acatagcatt tcccagcgat tctatcaccg tgccgtcttg ttctataagt ggctgtttgg
     3661 ccattcgttt taaatagtta tgtttaaaat actaaaattt gtaacttctt caataaaatc
     3721 aaatgttgat aaaacatcag ccttatcttt tctaacaact acattgtgtt cgtaatgtgc
     3781 tgacggaaga tgatcttttg ttttaatcgt ccagccatcg cttttctgat acacatcttt
     3841 aactcccaga ttaatcattg gctcaatggc taataccatt ccggcacgca attcctttcc
     3901 atatcctctt tttccatagt ttggcacttc tggtttctca tgaaggtttt ttccaagtcc
     3961 atgcccaacc aaatctctaa ctaccgaata gccaaaacct tcaacatact cttgaattgc
     4021 aaaaccgata tctccgatac gattacctcc gatagcaaca tcaattgcct tgtaaagcga
     4081 ttcaagggtt cgtttcatca acatcaatat ctctttcttc acttcaccca ctgggaatgt
     4141 ataagctgaa tccccaaaat agccattttt tttcactccg cagtcaatag aaacgatatc
     4201 gccatctttc aaaacataat cgcttgggat accatgaact acaacttcat taactgatgt
     4261 acacagcgtt cctgggaagc catgatatcc tttaaagcca ggtatagcat catgatctct
     4321 gataaactcc tcagcaactt tgtcaagttt attagtagtt actccgggtt tgat
//
LOCUS       DAOOAI010000129         4190 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_23804_length_4190_cov_4.651270, whole genome shotgun sequence.
ACCESSION   DAOOAI010000129 DAOOAI010000000
VERSION     DAOOAI010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4190)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4190
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23804_length_4190_cov_4.651270"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(88..642)
                     /locus_tag="PK345_08065"
     CDS             complement(88..642)
                     /locus_tag="PK345_08065"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HPX35007.1"
                     /translation="MKRDDALELLHRYVANPNLRKHCYASEAVMCALAKRLGEDPQRW
                     GLAGLLHDLDVELVNAAPEIHGRETARILLEYGLDEEIVEAVLLHNEQAADRPRTTVF
                     HHALAAGETITGLITATALVYPDKKLASVKPRSVEKRMKEKNFAASVNRDHIMECEKI
                     GLSLGEFIEICLEAMRSISDVLEM"
     gene            724..1242
                     /locus_tag="PK345_08070"
     CDS             724..1242
                     /locus_tag="PK345_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX35008.1"
                     /translation="MANTIFEEKQKFNQPWIRICLVLLIAIFTWGFIQQVVMGIPFGN
                     NPGPDWIFLPIFLLVGGLIIFFSSMKLITKVSKSGISYCFTPFHSKEKLITWDRVKSA
                     KVIDYRPIREYGGWGVRYGLKGKALNVRGKHGIEIEFFSGSNLLIGTQKPEELKNTLK
                     ELKESGVIAINN"
     gene            1322..2461
                     /locus_tag="PK345_08075"
     CDS             1322..2461
                     /locus_tag="PK345_08075"
                     /inference="COORDINATES: protein motif:HMM:NF012656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HPX35009.1"
                     /translation="MNFDFSEEQIMIRQAARDFAKKLESDVIERDCKAEYPTQHVKEL
                     SDLGFMGISVDPKYGGGGMDTISYLLAMEEISKIDSSVGVIMSVHNSLVMSGVEKYAS
                     DYLKDKYLPVMVTGEKIGAFLLSEPEAGSDATSQKTSALDMGDHYLLNGTKNWITSAG
                     TASAYIVFGQTYPEKRHRGINVFIVDRETPGISLGQHEDKMGMRSSDTHSVMFTDVKV
                     PKENRIGEDGFGFKFAMKALEEGRVGIAAQAVGIAQGAFERAVRYAKERKAFGKPIAE
                     HQAIAFKLADMAVKIENARNLYLKAGWLKQNKKPYAQYSAMAKLYAADIAMEVTTEAV
                     QILGGYGYVREYHVERLMREAKLTQIYEGTSEIQKIIISRQILAD"
     gene            complement(2532..3353)
                     /locus_tag="PK345_08080"
     CDS             complement(2532..3353)
                     /locus_tag="PK345_08080"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HPX35010.1"
                     /translation="MGKLGKLWKAFTSIVRQPSLLNLIIDTNQNWYKYVEKRYGLGAG
                     LPVAELSTLFPDFRANIPTFAFLDGGSLPTDIALLMKLAQRIPDCSYFEIGTWRGESV
                     VNVANIAKECYTLNLPDEEMRKAGQPENFIELIGFFSKDKKNITHLKGNSFDFDFVGL
                     NKKFDLIFIDGDHHYEAVKNDTKKIFESLVHDKSVVVWHDYARSPEVVRFEVFAGILD
                     GMLPQRHRYLYHVGNTLSAIYYPSEALAKHPLINPTKPNHYFELDLKLNPIVKKE"
     gene            3504..3884
                     /locus_tag="PK345_08085"
     CDS             3504..3884
                     /locus_tag="PK345_08085"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HPX35011.1"
                     /translation="MKLFDKIKLHSKWLFATYVIVILLFVTLPINTVSLNTVTIISFR
                     GDYFLHAVTFIPWAFFGWLLRRKMVYWFIVGVIFAGGVEGIQYLLSYRAFNINDMIGN
                     IIGVVLGFIAFVLFHFYLPQNNDK"
BASE COUNT         1334 a          815 c          803 g         1238 t
ORIGIN      
        1 gaacagggat caacgacaaa ttacaaaaac caaaattaca cataagtcca cattttttta
       61 attagtataa gataggaaag gctcttttta catctccagc acatcactga ttgagcgcat
      121 tgcttcaaga catatctcta taaattcacc aagcgacaga cctatctttt cacattccat
      181 gatatgatcc cgatttactg aagctgcaaa gttcttttct ttcattctct tttctactga
      241 gcgaggtttt acactggcta actttttatc aggataaacg agcgccgttg ccgtaataag
      301 ccctgtgatg gtctcgccgg cggcaagcgc atgatgaaaa accgtagttc tcggcctgtc
      361 ggcagcctgt tcgttatgaa gaagaactgc ttcaacaatc tcctcatcta acccatactc
      421 caaaagtatt cttgccgttt cacgtccgtg tatctcaggc gcagcgttca ccaactcaac
      481 atccaaatcg tgcagtaagc ctgctaatcc ccatcgctgc ggatcttcgc ctaatctttt
      541 agccaatgca cacataacag cctcagatgc ataacaatgc tttctgagat ttggattggc
      601 aacatagcga tggagcaact ccaatgcatc atcccgtttc ataatttttt cctgcaaaga
      661 taaaccaaag cataattgaa taaaaaaaac tgttattttt gacaacaaga ataaatttta
      721 attatggcaa acacgatctt cgaagaaaag caaaaattta atcagccgtg gattagaata
      781 tgtcttgtat tattaatcgc aatatttaca tggggtttta tccaacaggt tgtcatgggc
      841 atcccgtttg gcaataaccc tggccctgat tggatttttc tgccaatatt tttgttagtg
      901 ggcggcctta ttatattttt ttcgagcatg aaactaatta ccaaagttag taagtcagga
      961 atttcttatt gctttactcc ctttcacagt aaggaaaaat taataacatg ggatagggta
     1021 aaaagtgcaa aagttattga ttatcgccca ataagggaat atggtggatg gggagtaaga
     1081 tatggcctaa aaggcaaggc tttgaatgta cgaggcaaac acggtattga gattgagttt
     1141 tttagcggaa gtaatctttt aatcggcaca caaaaacccg aagagttgaa aaatactctc
     1201 aaagagctaa aagagtccgg cgttattgct ataaataatt aaaacttatt tttgttcgaa
     1261 accaaaatag aacactgatt cagataaaaa gctttattaa ataaccaaaa ataatttcct
     1321 tatgaatttt gactttagtg aagaacagat tatgatcagg caagcggctc gtgattttgc
     1381 caaaaagctt gagtccgatg ttattgagcg cgattgtaaa gccgaatatc ccacacaaca
     1441 cgttaaagag cttagcgatt taggctttat gggaatatcg gttgatccga aatatggcgg
     1501 tggcggtatg gatactatct catatctgct tgccatggaa gaaatttcaa aaattgattc
     1561 ctccgtagga gtaataatgt cggtgcataa ctccttagtg atgtccggtg ttgaaaagta
     1621 tgccagtgat tatttaaaag acaagtatct gccggtaatg gtaaccggcg aaaagattgg
     1681 cgctttcctt ttgtcggaac ctgaagcagg gtcggatgcc acttcacaaa aaacctcagc
     1741 attggatatg ggagatcatt atttgctcaa cggcaccaaa aactggatca ccagcgccgg
     1801 cacagcatcg gcatatatcg tgttcggaca gacctatccc gaaaagcggc accgaggcat
     1861 caatgttttc attgttgaca gagaaactcc aggcatatca cttggccagc atgaagataa
     1921 gatgggcatg cgtagtagcg acacccattc agtaatgttt actgatgtga aggtaccaaa
     1981 agaaaacaga attggagaag atggctttgg ttttaaattt gccatgaaag cgctcgaaga
     2041 gggacgtgtt ggaattgctg ctcaagcagt aggcattgca caaggcgctt ttgaaagagc
     2101 agtaaggtat gctaaagagc gaaaagcatt cggaaaacca atcgccgagc atcaggctat
     2161 tgcgtttaaa cttgccgata tggcggtaaa gattgaaaat gcccgaaacc tttacctcaa
     2221 agccggttgg ttaaaacaaa acaaaaaacc atatgcacag tatagcgcca tggccaaact
     2281 ttatgctgcc gatatagcga tggaggtaac taccgaagct gtacagatac ttggtggata
     2341 cggctacgta agagagtatc atgttgaaag attgatgaga gaagctaagc ttacacagat
     2401 atacgaaggc acatcggaaa tacaaaagat cattatatca cgacagatat tagccgattg
     2461 agtttaatta aggctaagct ttaaaatact actcgttttt taagcctttg accatgttct
     2521 aaggtaagtc tttattcctt cttcactatg gggtttaatt tcaggtcaag ctcaaaatag
     2581 tgattaggtt tagtgggatt tataagagga tgctttgcca atgcttcaga gggataataa
     2641 atagctgaaa gtgtatttcc tacatggtag agataacgat gccgctgtgg cagcatccca
     2701 tcaagtatgc cggcaaagac ttcaaaacga acaacttcag gactcctggc ataatcgtgc
     2761 catacaacca ccgatttatc gtgtacaaga ctttcaaaaa tcttcttagt atcatttttt
     2821 acagcttcat agtgatgatc accatcaata aatatcagat cgaacttctt atttaaacct
     2881 acaaaatcaa agtcaaatga atttcctttt aaatgggtta tgtttttttt atctttagaa
     2941 aaaaaaccaa ttaactctat aaaattttca ggttgacctg ctttccgcat ctcttcatcc
     3001 ggcagattta atgtatagca ttctttggct atgttagcca cattcacaac gctttcgccc
     3061 ctccatgtcc caatttcgaa atatgaacaa tcagggatcc gctgagccaa cttcatcaat
     3121 aaggcgatat cggtgggtag agaaccacca tcgagaaaag caaatgtggg gatattagct
     3181 ctaaaatcag gaaatagagt actaagctct gcaaccggca acccagctcc taaaccataa
     3241 cgtttttcta catatttgta ccagttttga ttggtgtcaa tgataagatt taaaagtgaa
     3301 ggctgcctaa caatagaggt aaaagctttc caaagttttc ctaactttcc cataagattt
     3361 tgttttttac aaaagtaacg gttttgaaaa tattaaacag cgcgcataag gtattgcaga
     3421 aaaataatac ttttggcggt aataattaaa taataaccag tgcaaaaacc acttagttaa
     3481 aaacacacta ttctttttta taaatgaaac tgtttgacaa gatcaagctc cattcaaaat
     3541 ggcttttcgc aacctacgta attgtaattc tgttatttgt aactctaccc atcaacactg
     3601 tatcgctaaa tactgttacc attatcagct ttcgtgggga ttattttctg catgcggtta
     3661 catttatacc atgggccttt ttcggatggc ttttacgtcg taaaatggtt tattggttca
     3721 ttgtcggagt aatttttgca ggcggcgtcg aaggcattca atacctttta tcttatcgcg
     3781 ctttcaatat caacgatatg atcggaaata tcattggcgt cgttttagga tttattgctt
     3841 ttgttttgtt ccacttttac ttaccgcaaa ataacgacaa atagccttaa ataggctttg
     3901 tcgaataagt caggtgttta tcgtaaaaaa tatttttttt tgaccgattg agagcattaa
     3961 aataacctac ccaattgttt ttgaacaatt tctcaactca tgggatcaag ttcttctact
     4021 ccacgccaat agcgcaatgt agtgaataat gagctatcaa agcatcttta taggcgtatt
     4081 gactcaatca gagaaaaatc agtttattat atatttttca ccaaacctta cgtagcagca
     4141 ctttaagcgc tgtaaacaaa aaaagctcca ccaaccggca gagctttttt
//
LOCUS       DAOOAI010000130         3926 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_25821_length_3926_cov_5.343064, whole genome shotgun sequence.
ACCESSION   DAOOAI010000130 DAOOAI010000000
VERSION     DAOOAI010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3926)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3926
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25821_length_3926_cov_5.343064"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            284..1636
                     /locus_tag="PK345_08090"
     CDS             284..1636
                     /locus_tag="PK345_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009135346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)-translocating NADH-quinone reductase
                     subunit A"
                     /protein_id="HPX35012.1"
                     /translation="MSETITIKQGLDIKLLGGAHQEISHVDSGFYAIKPGDFIGVFPK
                     LLVQEGDKVKAGTQLFYDKTWDFLRLTSPVSGIVKEIRRGAKRVLLEIIIEPDTVQEY
                     EDFGKSDPNTLTKEQIIEKLVQSGVWPMLRQRPYNVVANPNDQPKAIFISAFDTSPLA
                     PDYNFIVNGEAEAFQTGIDALAKLTSGKIHLNLKRSESISEFFTKTSKVQFNYFEGPH
                     PAGNVGVQINKIDPINKGEVVWHLRPQEVLTIGRLFLKGYFDAEKIIAVTGSEVIKPA
                     YVKTKIGACIQDILKNNLKEGNLRFISGNPLTGTKIDQQGFISFYDSQITVLPEGDYY
                     EFIGWALPGFSKFSHSRTFFSWLHPKNKKYRLDTNLHGGHRALVMTGEMEKVFPFDIY
                     PLQLLKAIIVEDVEAMENLGIYEVVEEDFALAEYLDTSKTEIQALVRKGLDLMRKEMS
                     "
     gene            1661..>1951
                     /locus_tag="PK345_08095"
     CDS             1661..>1951
                     /locus_tag="PK345_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RnfABCDGE type electron transport complex
                     subunit D"
                     /protein_id="HPX35013.1"
                     /translation="MNAIRKYLDKIKPNFQKGGKLEYLHSTFDAFETFLFVPNKTTFS
                     GSHIRDYMDMKRTMSMVVLALIPILLFGMWNVGYQHFIANSVESGFWANMLFG"
     assembly_gap    1952..1961
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1962..2816
                     /locus_tag="PK345_08100"
     CDS             <1962..2816
                     /locus_tag="PK345_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018336167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit B"
                     /protein_id="HPX35014.1"
                     /translation="ILIVSYVVGLGIEFAFAQIRGHEVNEGFLVTGFLIPLIVPPDIP
                     LWMVALATAFAVIFGKEVFGGTGMNILNPALTARAFLFFAYPQDMSGEKVWVADALSG
                     ATPLGKLMVNEPLASLPSPSDMFFGFIPGSVGETSTLLILLGGLFLIYTGIASWKIII
                     SVFAGGYALALLFNFLGPIIAPENVYMAMPAYYHLIIGGFAFGAVYMATDPVTAAQTN
                     HGKWIYGLLIGILAVLIRVLNPAYPEGMMLAILLMNVFAPLIDYYVVRANINKRLKRF
                     QKTQLATT"
     gene            2830..3597
                     /gene="nqrC"
                     /locus_tag="PK345_08105"
     CDS             2830..3597
                     /gene="nqrC"
                     /locus_tag="PK345_08105"
                     /EC_number="7.2.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:ubiquinone reductase (Na(+)-transporting)
                     subunit C"
                     /protein_id="HPX35015.1"
                     /translation="MFSNRYIYIYASVMVIIVAAALSTASELLKPYQNKNIENEKFQG
                     ILSAAEVDVPRSQANEAFEEYIVEELAINSEGDVVCRYVDGKLIEGDVRPFNIVLKEE
                     LKLKKDFKYGKAKRNPKWPLYIMDIGGELKYVVPLQGVGLWGAIWGNIAFDSDFNTVA
                     GTSFDHKSETPGLGAEINTKEFQNQFKGKQIFDQNGNFVSVSVVKGGVANSAINPIHG
                     VDAVSGGTITSNGTSEMLKDCIENYVSYIKKNINNNE"
     gene            3590..>3926
                     /locus_tag="PK345_08110"
     CDS             3590..>3926
                     /locus_tag="PK345_08110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009134999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rnf-Nqr domain containing protein"
                     /protein_id="HPX35016.1"
                     /translation="MSSTREPLFSSKNIKLLTTPLGRENPITVQVLGICSALAVTVKM
                     QPAFVMAISVIAVLAVGNVVISLLRKTIAPRIRIIVQLVVAAALVILVDQILKAFVYD
                     ISKQLSVFVG"
BASE COUNT         1172 a          645 c          823 g         1276 t
ORIGIN      
        1 aaaaatagaa caaaattctt aggtttttag tcagaaaata actacgaacg atttaatttt
       61 ttaaatattt acgaattcac aataaaattt agttgggatt taattaaaaa tcattgttgt
      121 ccgataaaaa attaaggctc ttaaaccctg ttattaaggc tatattgatc aagttagtgc
      181 aataatatta tttattaaac aattcagttc ttaaatagaa tttttatgtt tgtaaccttt
      241 tttaaagtta gttgaacaac ttacaacaat ttcaaaaaat attatgtcgg aaactattac
      301 cattaagcaa ggtcttgata ttaaattact tggcggcgct catcaggaga ttagtcatgt
      361 agattctggc ttttatgcga tcaagcctgg cgattttatc ggtgtctttc ccaagttgct
      421 tgtgcaggaa ggcgataagg tgaaagccgg gacacagtta ttttatgata aaacatggga
      481 ttttctcagg ttaacctctc cggtaagcgg tattgtcaaa gaaattaggc gtggcgctaa
      541 gcgtgtttta cttgaaatta ttattgagcc ggacacagtt caggaatatg aagatttcgg
      601 taaaagcgat cctaacactt taaccaagga acagatcatt gaaaaattag tgcaaagcgg
      661 tgtatggcca atgcttcgtc aacgtcctta taacgttgtt gctaatccta acgatcagcc
      721 gaaagcgata ttcatttcag ctttcgatac cagtccatta gctcctgact ataattttat
      781 tgttaatggg gaggcagagg cgtttcaaac aggaattgat gcattggcaa aactaacaag
      841 cggcaaaatt catcttaatt tgaaacgtag cgaatcaata tctgaatttt ttactaaaac
      901 ctcaaaagtt cagtttaatt attttgaagg gcctcatcca gcaggcaatg taggagtaca
      961 aataaataaa attgatccta taaacaaagg ggaggttgtt tggcaccttc gtccacaaga
     1021 agtgcttacg atcgggcggc tattcctgaa aggatatttt gatgctgaaa agattattgc
     1081 cgtaacaggg tcggaggtta ttaagccggc ttatgtaaaa acaaaaatcg gtgcatgtat
     1141 tcaggatatt ttaaaaaata atctgaaaga gggtaatctt agatttattt ccgggaatcc
     1201 attgaccggg actaaaattg atcaacaggg gtttattagt ttttatgatt cacaaattac
     1261 cgttcttccc gaaggtgatt attatgagtt tatcggttgg gctttaccag gtttttccaa
     1321 attcagccat tccagaacct tttttagttg gttgcaccct aaaaacaaaa aatacaggct
     1381 cgacaccaat cttcatggtg ggcatagggc gctggtaatg acaggcgaga tggaaaaagt
     1441 atttcctttc gatatttacc cattgcaatt gcttaaagct atcattgttg aggatgttga
     1501 agccatggaa aaccttggta tttacgaggt ggttgaagag gattttgcct tggccgaata
     1561 ccttgacact tcgaaaacgg agattcaggc tcttgtacga aagggtttgg atctgatgag
     1621 aaaagaaatg agttaacaat tttaaaatca ggatatcaga atgaatgcta tcagaaaata
     1681 tctggataag attaagccga attttcagaa agggggtaaa ttagagtatc tccattctac
     1741 attcgatgca tttgagactt ttctttttgt acctaataaa acaacattta gtggaagcca
     1801 tattcgtgat tatatggata tgaagcgcac catgagcatg gttgttctgg ctctgatacc
     1861 tatactgctt tttggaatgt ggaacgttgg ttatcagcac tttattgcta atagcgttga
     1921 gtctggtttt tgggcaaata tgctctttgg cnnnnnnnnn nattttaata gtttcctatg
     1981 tagtaggttt ggggattgaa tttgcctttg ctcaaattag gggccatgag gtaaatgaag
     2041 gatttcttgt aacaggtttt ttgattcctc ttattgttcc acccgatatt cctttgtgga
     2101 tggttgcact tgcaacggct tttgcagtaa tatttggtaa agaggtgttt ggcggtactg
     2161 gtatgaacat tcttaatccc gcattaactg cacgtgcatt tttatttttt gcctatccgc
     2221 aggatatgtc aggagaaaaa gtttgggttg ctgatgctct ttcgggtgct acaccacttg
     2281 ggaaattgat ggtcaacgag cctcttgctt ctttacccag tccatccgat atgttttttg
     2341 gttttatccc tggatcggtt ggagaaacat ccactttact cattcttctt ggtggcctat
     2401 ttttgattta caccggtata gcaagctgga agattataat atctgttttt gccggtggtt
     2461 atgcgcttgc attactgttc aattttcttg ggccaataat agcgccagaa aatgtttata
     2521 tggctatgcc ggcatactat caccttatta ttggcggatt tgcttttggc gctgtttata
     2581 tggctactga tcctgtaaca gcagctcaaa ccaatcatgg aaagtggatt tatggcctgt
     2641 taatcggtat tttggccgtt cttatcagag tattaaaccc cgcctatccg gaaggaatga
     2701 tgctggctat tttactgatg aatgtttttg ctccacttat tgattattat gtggttagag
     2761 caaacattaa caaaaggctt aagagatttc aaaaaacgca gttagcaaca acataaaatt
     2821 taaatcaaga tgtttagcaa ccgatacatt tatatttatg cttccgtgat ggttattatt
     2881 gttgcggcag ctttatctac agcatccgag cttctgaagc cttatcaaaa taaaaatatc
     2941 gaaaatgaaa aatttcaggg aattttaagc gctgcagaag tagatgttcc acgatcacaa
     3001 gcaaatgaag cttttgagga atatatagtt gaagagttgg ctattaattc cgaaggtgat
     3061 gtagtttgcc gatacgttga cggcaagttg atcgaaggtg atgtgcgccc ttttaacatt
     3121 gttttaaaag aggaacttaa gctaaaaaag gatttcaaat atggtaaggc gaaacgtaac
     3181 cctaaatggc ctctttatat catggacatt ggtggcgagc ttaaatatgt agttccgtta
     3241 caaggtgtgg gtttgtgggg agcgatttgg gggaacatag ctttcgatag tgatttcaac
     3301 accgtggccg gcacttcatt tgaccataaa tccgaaacac ctggacttgg cgctgaaatt
     3361 aacacaaaag aattccaaaa tcagtttaaa gggaagcaaa tttttgatca aaatggaaat
     3421 tttgtttccg tttctgtagt taaaggtggt gtagccaata gcgctattaa tcccatacat
     3481 ggcgttgatg cagtttcagg aggtactatt actagtaacg gcacttctga aatgttaaaa
     3541 gactgcattg aaaattatgt ttcatatatc aaaaaaaata ttaataacaa tgagtagtac
     3601 aagagaacca ttattttcat ctaagaatat aaagctgctt accacgcctt tgggtcgtga
     3661 aaatcctatt acggtacaag ttttaggtat ctgttcggcc cttgcagtta ccgttaagat
     3721 gcaaccggca tttgttatgg ctatttctgt aatagcagta ctggctgttg gtaatgtggt
     3781 aatatcactt ttaaggaaaa ccattgcgcc acgtatccgt attatcgttc agcttgtggt
     3841 agcagcagct ttagtaatcc ttgttgacca aatacttaaa gcttttgttt atgatatcag
     3901 caaacaactt tcggtatttg tgggat
//
LOCUS       DAOOAI010000131         3684 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_28017_length_3684_cov_5.496556, whole genome shotgun sequence.
ACCESSION   DAOOAI010000131 DAOOAI010000000
VERSION     DAOOAI010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3684)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3684
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28017_length_3684_cov_5.496556"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..516)
                     /gene="coaE"
                     /locus_tag="PK345_08115"
     CDS             complement(<1..516)
                     /gene="coaE"
                     /locus_tag="PK345_08115"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HPX35017.1"
                     /translation="MLRVGLTGGIGSGKTLVAEVFKSLSVPVFNADVEGKKLLFAPHV
                     KEQLRTLFGDHIFSCGEVDRSILANLVFDKPDKLTQLNAVIHPEVRKMFELFAKKNSS
                     SPYVLHEAAILVETGYYKQLDKLILVVADADLRVDRVMKRDNVTREQVISRMKNQWDD
                     EKKILQSDFVIY"
     gene            complement(516..1499)
                     /locus_tag="PK345_08120"
     CDS             complement(516..1499)
                     /locus_tag="PK345_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YbbR-like domain-containing protein"
                     /protein_id="HPX35018.1"
                     /translation="MSKYERIKSSENKNYRYRTTVFIICLLVSALLWLTIKFSSDYTV
                     EIPYSISLKGIPEGKVLVNQPDSVLRINVKASGYALLKCRYLSRHDSYEVNVSQWIGR
                     ISAGTSDATINTLLIAQHIKEERSLPGIIEFVSPEELSLKFDRGVSKQVPIIPDVSYS
                     FAQQYFAYDSLIIEPQLATIYGTTDDIDRVHFLKTNALKFDDLSESIDTMVNILNPRS
                     NAIVIEPEQAWISLLVEKFTEAEVEVAVKRGKGFEQTRVKFFPERVKVVYLIALKDFK
                     NIDPEMFTCTVNMPDDGAPNGKRLNVEVSDHSSSIKIQYVMPSDVEYIILK"
     gene            complement(1601..1951)
                     /gene="yajC"
                     /locus_tag="PK345_08125"
     CDS             complement(1601..1951)
                     /gene="yajC"
                     /locus_tag="PK345_08125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HPX35019.1"
                     /translation="MISSILNFILLSQNPGGNEGGGMSFLFMILLMIVVFYFFMIRPQ
                     TKKAKQQKNFRENLKKGDKIITIGGIHGKILEVKETTLIIQTEGEGRIKIEKSAAAVD
                     ASSQLDEQNALPQK"
     gene            complement(1986..2441)
                     /locus_tag="PK345_08130"
     CDS             complement(1986..2441)
                     /locus_tag="PK345_08130"
                     /inference="COORDINATES: protein motif:HMM:NF019242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1573 domain-containing protein"
                     /protein_id="HPX35020.1"
                     /translation="MRAFLFFSFLLFAVTACGYRHGDKLPVDMVKNPKTASGEDEGVK
                     MPVIAFEKQTHDFGRLVMGEKVSYTFRFTNIGEADLIISHVSTNCGCTVPEYTKEPVK
                     PGETGNLSVTFDSKNRKGFQNKTITVITNTQPNSSELNITAMVAIPVQK"
     gene            complement(2544..3263)
                     /locus_tag="PK345_08135"
     CDS             complement(2544..3263)
                     /locus_tag="PK345_08135"
                     /inference="COORDINATES: protein motif:HMM:NF017026.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HPX35021.1"
                     /translation="MGDFVFRHFRILHGNSTMKVGTDAVLVGALTKIHPSVRTILDVG
                     TGCGIIALMLAQRSNAEIVAIDIDKPSVEEATINFRNSPWPDRLKPVLAAFQHFSSTS
                     EIRYDLIVSNPPFFQDCQLPLRESLSIAKHNKRLNFEELLQGAERLLNDDGSISLILP
                     VNESKSFLEKAGDCSFFPNEIIEIVPIKGKPVNRILLTVKRKKSPNPLIQTLTLRNND
                     GKFSDDYKMLTKDFHPPEYFY"
     gene            complement(3286..>3684)
                     /gene="rimM"
                     /locus_tag="PK345_08140"
     CDS             complement(3286..>3684)
                     /gene="rimM"
                     /locus_tag="PK345_08140"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HPX35022.1"
                     /translation="FIEIDGGLVPFFVIEASLRGDNSLVVMLEDYHLPEKALKLVGCK
                     VYIKIEEIYDSEDEFLFYPQLIGFKVIDKDKGEVGVVKEVSESMHQILLTVMKGKKEI
                     LIPLAEEFITKVNRHKKELFMVLPDGLIDL"
BASE COUNT         1135 a          773 c          617 g         1159 t
ORIGIN      
        1 ataaatcaca aaatccgatt gcagtatttt cttttcatca tcccattggt ttttcattcg
       61 gcttatcacc tgctcacgag ttacattatc acgtttcatc acacgatcca ctcgtaagtc
      121 tgcatcggcc accacaagga tcaatttatc aagctgctta taatagcctg tctctacaag
      181 gattgcagct tcatgcaaaa cataagggga agagctgttt ttctttgcga agagttcaaa
      241 catctttctc acttcggggt gtatcactgc atttagctgg gttagcttat caggtttatc
      301 gaaaactaaa tttgccagta tgctacggtc aacttcgccg caactaaaga tatgatctcc
      361 gaagagagtt cgtaattgct cttttacatg tggggcaaat agtagctttt ttccttcaac
      421 atcggcatta aacacaggca ccgacaaaga tttgaacacc tcagccacta atgtcttacc
      481 actgcctata ccacccgtga gcccaacgcg cagcattatt ttaaaatgat atattcaaca
      541 tcggatggca taacgtactg tatttttata gatgaagaat ggtcggatac ttcaacattt
      601 aatcgcttac catttggcgc tccatcatcc ggcatattta cagtgcatgt aaacatttcg
      661 ggatcaatat ttttaaaatc tttaagcgct atcagatata caaccttcac cctttccgga
      721 aaaaacttaa ctctagtttg ctcaaagcct ttacctcgct ttacagcaac ctcaacctca
      781 gcttcggtaa acttttcaac taacaggctg atccatgctt gttcaggctc tataacgata
      841 gcgttagaac gtggattaag gatgttaacc atagtatcaa ttgattcgga caaatcatca
      901 aactttaaag catttgtttt caaaaaatgg actctatcta tgtcatcagt agtaccataa
      961 atagtagcca gttgaggttc aatgataaga gagtcgtaag caaaatactg ttgagcgaag
     1021 ctataactca catcggggat gattggcacc tgcttcgaaa ctcctctatc aaatttaaga
     1081 ctcaactctt cgggagagac aaattctata atccccggta atgatcgttc ttccttaata
     1141 tgctgagcaa taagtaaagt attgatggtt gcatcggatg ttcctgccga tatgcgtcca
     1201 atccattgac ttacgttaac ttcataagaa tcatgcctcg acagatatct acactttaat
     1261 aaagcgtatc ctgaagcttt tacattaatc cgaagcacgc tgtcaggctg attgactaat
     1321 actttgcctt caggtattcc ttttaagctt attgagtaag gtatttcaac tgtatagtca
     1381 ctgctaaact ttatcgtaag ccaaagtaga gccgaaacga gtaggcaaat gatgaaaacc
     1441 gttgttcggt aacgatagtt tttgttctct gaacttttta tacgttcgta tttactcatt
     1501 tgaaaatgaa acaaataatt tgcaaagaaa tacaaaatcc atccatacta tgcttagtcg
     1561 tataggtttc tataattcaa aaacagctta tgtcaaagac ttatttttga ggtaaggcgt
     1621 tctgttcatc aagttgtgaa ctggcatcaa ctgccgctgc acttttttct attttaattc
     1681 tgccttcgcc ttccgtctga ataatcaggg tggtctcttt tacttcgaga atcttaccat
     1741 gtattccacc aatggtaatg atcttatctc cttttttcag attttcgcga aagttctttt
     1801 gctgtttggc ctttttcgtc tgtggcctga tcataaaaaa gtaaaaaact acaatcatta
     1861 gcaggatcat aaaaagaaaa gacatcccgc cgccttcgtt accgccagga ttttggctta
     1921 acaaaataaa attcaaaatt gaacttatca taaaagtata ttattaaatt ataaaacctc
     1981 ttttatcatt tttggactgg tatagcaacc attgccgtga tgtttagttc gcttgaatta
     2041 ggttgagtgt tggttataac ggttatggtt ttgttttgaa agccctttcg atttttacta
     2101 tcaaaggtta ctgaaagatt tcctgtttct ccgggcttta ccggttcttt tgtatattcg
     2161 ggaactgtac aaccacaatt agtgctgacg tgtgatatga ttaggtcggc ctcgccaatg
     2221 tttgtaaacc gaaatgtata actcaccttt tcacccatta ccaatctgcc aaaatcgtga
     2281 gtttgtttct caaaagcgat taccggcatc ttaacgcctt catcttcgcc ggaagccgtt
     2341 ttaggatttt taaccatatc aacaggcagc ttatcaccgt ggcgatagcc acaagcagta
     2401 accgcaaata aaagaaaact gaaaaaaaga aaagctctca ttgcttttta cttacaacca
     2461 aaaattcaga taactgcaaa aatacacaaa tccgataatc atcctattta ttgaaaatat
     2521 aggaatcaat aatacgagca aggttaataa aaatactcag gcggatggaa atcttttgtc
     2581 aacatcttgt aatcatccga aaacttccca tcgttatttc tcaatgttag cgtttgaatt
     2641 aatggatttg gggatttttt tctctttacg gtaaggagaa tacgattaac cggtttgcct
     2701 ttaattggga caatttcaat tatctcattc ggaaaaaatg aacaatcccc ggctttttct
     2761 aaaaatgatt tcgactcatt taccggtaaa ataagtgata ttgatccgtc atcattcagc
     2821 aatcgctcag cgccctgtaa aagctcttcg aaattgagtc ttttgttgtg ttttgcaatg
     2881 cttaagctct ctcttaaagg caattggcaa tcctgaaaaa atgggggatt acttacaatc
     2941 aagtcataac gtatttcaga ggtagaggaa aaatgctgaa aagcggccaa aacaggtttt
     3001 aaacggtcag gccagggtga gtttctgaag tttatagtag cctcctctac cgagggttta
     3061 tcaatatcaa ttgcgactat ctcggcattc gacctttgag ctaacatcaa tgctataatg
     3121 ccgcatcctg ttcccacatc gaggatggta cgcaccgaag gatgaatttt agttagagcg
     3181 cctaccaaaa cagcatcagt acctaccttc attgtagaat tgccatgcaa tatcctgaaa
     3241 tgtcgaaaca caaaatctcc cataaaaaca aaaagatagt agatattaaa gatctatcag
     3301 cccatctggt aaaaccataa agagttcttt tttatgccgg ttaactttgg taatgaactc
     3361 ctcagcgagc ggtatgagta tctccttttt acctttcatt acagtaagta aaatctggtg
     3421 catactctcc gacacttctt ttacaacacc cacctctccc ttatctttat caattacctt
     3481 aaatccaatt agctgaggat aaaaaagaaa ttcatcttca ctatcataaa tctcttcaat
     3541 tttgatataa accttacagc caaccaattt taaagctttt tcaggcaaat gataatcttc
     3601 caacatcaca acaagggagt tatcccctct caacgatgct tctataacaa aaaaaggaac
     3661 taacccaccg tcaatttcaa taaa
//
LOCUS       DAOOAI010000132         3666 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_28205_length_3666_cov_4.605649, whole genome shotgun sequence.
ACCESSION   DAOOAI010000132 DAOOAI010000000
VERSION     DAOOAI010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3666)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3666
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28205_length_3666_cov_4.605649"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..267)
                     /locus_tag="PK345_08145"
     CDS             complement(<1..267)
                     /locus_tag="PK345_08145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX35023.1"
                     /translation="MKNSERVIYELLLTIIAFLLAGFAGGKITLNLQLVPSSFVNHSV
                     MFILSIVLIGSLRKYVNYKIAWPKFKKIWKPILFGFLSAVIINSL"
     gene            complement(307..513)
                     /locus_tag="PK345_08150"
     CDS             complement(307..513)
                     /locus_tag="PK345_08150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX35024.1"
                     /translation="MKKLKTGITLIVLGNVLYVSKDFFANIGSSAFGDFTEGFLLGMG
                     VGLNVIGIILVFIHLAREAKKDQQ"
     gene            complement(528..1349)
                     /locus_tag="PK345_08155"
     CDS             complement(528..1349)
                     /locus_tag="PK345_08155"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPX35025.1"
                     /translation="MDLRKAKEKLDYYNKIELDLSYGKIGYLNAGEGEIILSVHGIFG
                     GYDQAYENIKSRVGKNRIIAPSRFGYLGSDIKGKGTPKEQAEAFNELLDSLEIDKVFV
                     LGASAGGTPAIRFALDYPERVKGLILFCSAMPVNEEPDKYSEYQAPPQPLLSNYAMYL
                     ISPLLPWVIGMPSSTVKDIMPVGARKEGVILDGKLTNPDMERNFHDYPMEELKMPTII
                     VHSEDDDVASFRKVEAAKHRFPNLTLVTFPDGGHMMKGHGAEIDRALDDFINTLK"
     gene            complement(1440..2204)
                     /locus_tag="PK345_08160"
     CDS             complement(1440..2204)
                     /locus_tag="PK345_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX35026.1"
                     /translation="MIKTFIKEKFELIERNRGQRVKFFTEEAIKLLPEPLKKYLRVCG
                     YIDRPIPVNANVCWSESWLKISPKNKWKKLRTLQFNSVKPLGRSAYMKILSMPVAARD
                     LYHDGYGEMKVKLMNWIKVAYDNSKEVAQSALITTFCEFMLIPSYLLSDNVKWEEINN
                     NSVKGTLADHGIEVSGIFHFNDKGFFTHFETLDRYFTVAKNKYEKVKFSVVVEGYKAQ
                     KNLKIGENVKVMWHLPEGDYKYYKGTIDKIEFNVFE"
     gene            complement(2201..3355)
                     /locus_tag="PK345_08165"
     CDS             complement(2201..3355)
                     /locus_tag="PK345_08165"
                     /inference="COORDINATES: protein motif:HMM:NF012770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HPX35027.1"
                     /translation="MRNRVVLVIVIIVMAVILAAGIFAYRNLTYDYLNEHFLEKSGFK
                     AGFTENVAKLEDGSEIYYIKGPENGPSLLLLHGQQVNCYDYAKALPKLSKHFHVFALD
                     YYGHGHSSKNPAKYSAVKIGNDLVWFIENVIKEKVYISGHSSGALIAAYVSAKAPEYI
                     IATILEDGPFFSTLPKRAEKTIAWLEFRNMYNYLNQNEVDNYIEYSLENDYMREVINS
                     MHPDAWQKIMVEPALKYLDRHPGGIPKIWYYPPGLKINSLYALNSNLQDGTGKYDLRF
                     GVTFYDFSWFEGFDQEEILKNIQSPTLVMHVAPNEITEPGYYDKKGILLAAMDEKDVQ
                     KVVDLVPESKYLGGFQSAHNIHDDLPEEYIQVLLDLKTQIAENDNKLNLK"
     gene            complement(3352..>3666)
                     /locus_tag="PK345_08170"
     CDS             complement(3352..>3666)
                     /locus_tag="PK345_08170"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4386 family protein"
                     /protein_id="HPX35028.1"
                     /translation="EAVALLAIFLKGNLLGDLGYKLAMLVLGIGSIPMCLTLLKYKIT
                     PSWLAIWGAIGYAVFAFGFLMELFGKEWSIYLLGLGGLWEVTFGIWLIVRGSQRKKRV
                     RV"
BASE COUNT         1062 a          818 c          610 g         1176 t
ORIGIN      
        1 aagagagttt attattactg ctgacaaaaa tccaaacaat atcggcttcc atatcttttt
       61 aaattttggc caggcgattt tgtaatttac atacttccta aggctaccaa tcaacactat
      121 agaaagtata aacataacag agtggtttac aaaggaagaa ggtaccaatt gaaggttcaa
      181 tgtaattttt ccaccggcaa aaccggctaa tagaaatgcg attattgtta ataatagctc
      241 ataaattact cgttcgctat ttttcatctt tttcacttaa ttattccgtt cacctaccca
      301 cctcaactat tgttgatcct tttttgcttc tctggccaga tggataaaaa cgagtattat
      361 accaattacg ttgagaccga ctcccattcc caataaaaac ccttcggtaa aatctccaaa
      421 agcacttgac cctatattgg caaaaaaatc ttttgataca taaagcacat tacccaatac
      481 tatgagcgta atgcctgttt ttaatttttt catttgataa tcctccatta tttaagtgta
      541 tttataaaat catctaaggc tctatctatt tctgcgccat gccctttcat catatgccca
      601 ccatcaggaa atgttaccaa tgtgaggtta ggaaaacgat gtttggctgc ttcaaccttt
      661 ctaaaactcg ccacatcgtc atcttcagaa tggacaatta tagttggcat ttttaattct
      721 tccataggat aatcatggaa attcctttcc atatcaggat tggtcaattt cccatccaaa
      781 ataactccct cttttcttgc tccaacaggc ataatatctt ttaccgtgga tgaaggcatt
      841 cctataaccc acggcaaaag cgggctgatt aaatacattg catagttgga cagaagcggt
      901 tggggaggtg cttgatactc cgaatattta tccggctctt cattgacggg catagcagaa
      961 caaaagagta ttaaaccttt tacccgctca gggtaatcca gagcaaatct gatagcagga
     1021 gtaccacctg cggaagcccc taatacaaat accttgtcaa tctccaaact atccagcaat
     1081 tcattaaaag cctcggcctg ctcctttggt gtaccttttc cctttatatc agaacctaaa
     1141 tatccgaaac gcgatggtgc aattatgcga ttttttccaa ccctactctt aatattctca
     1201 taggcttgat cataaccgcc aaaaattcca tgaacggata aaattatttc accttctcct
     1261 gcatttaaat agccaatctt gccgtaggat agatccaatt ctattttgtt ataataatcc
     1321 agtttttcct tggccttcct caaatccata tgacatctga caccttgtat cagaaggtac
     1381 gcggctaaga tcagtatgat ggcaattaaa actttcttca tctctttttt acttacgctt
     1441 tactcaaaaa cgttgaactc tattttatcg atggttcctt tgtagtattt gtaatcgcct
     1501 tccggcaaat gccacataac cttgacattt tcacctattt ttaaattctt ctgggccttg
     1561 tagccttcca caacgaccga aaacttaact ttctcgtatt tgtttttggc aactgtaaaa
     1621 tagcggtcta atgtctcaaa gtgggtaaaa aatcccttgt cgttaaaatg gaaaataccg
     1681 gaaacttcaa tcccgtgatc tgcgagcgtt cctttaacgg aattattatt tatctcctcc
     1741 cattttacat tatccgaaag cagataactc ggaataagca taaattcaca gaaagtggtt
     1801 atcagtgccg attgtgccac ttctttgctg ttatcataag ctacctttat ccaattcatc
     1861 agcttaacct tcatttctcc ataaccatca tgatataaat cccttgctgc cacaggcata
     1921 ctcaaaattt tcatgtaggc ggaccttccc agcggcttca cagagttgaa ttgcagcgtc
     1981 ctaagttttt tccacttgtt tttaggagag attttcaacc agctctcact ccaacaaaca
     2041 ttggcattta cggggatcgg tctgtcgata taaccgcaaa cgcgaaggta ttttttcaaa
     2101 ggttcaggca gaagttttat tgcctcttcg gtaaaaaatt taactctttg acccctattt
     2161 ctttcaataa gttcaaattt ctctttaatg aatgttttta tcattttaaa tttagtttat
     2221 tgtcgttttc tgctatttga gtctttaaat cgagtaaaac ctgaatatac tcttcaggca
     2281 ggtcgtcgtg tatattatgt gcggattgga aacctcccaa gtatttgctc tcaggcacta
     2341 aatcgacaac tttttgaaca tctttttcgt ccattgccgc cagcaaaatt ccctttttat
     2401 cgtagtatcc cggctctgtt atctcattgg gagccacgtg catcaccaga gtgggggatt
     2461 gaatgttttt caatatttct tcctgatcaa atccttcaaa ccaggagaaa tcgtaaaatg
     2521 taactccaaa tcttaaatca tatttccctg taccgtcttg caggttggaa tttagtgcat
     2581 atagagagtt tattttcagc cccggcggat aataccagat tttggggatt cctcccggat
     2641 gcctgtccaa atattttaac gcgggttcta ccattatctt ctgccaggca tcgggatgca
     2701 tactattgat aacttcccgc atatagtcat tctccaatga atattctata taattatcta
     2761 cctcattttg attgagataa ttatacatat tccggaattc cagccatgct atggtttttt
     2821 ccgctctctt gggaagggta gaaaagaaag gtccgtcttc caggattgtg gcgataatgt
     2881 actcgggagc tttggcagaa acatatgcgg caatcaaggc ccctgatgaa tgccctgata
     2941 tatatacctt ttccttaatg acgttttcga tgaaccaaac cagatcgttt cctattttaa
     3001 cggcactgta tttagccgga ttttttgatg agtgtccatg tccgtagtaa tcgagagcaa
     3061 aaacatgaaa atgttttgag agttttggga gtgctttcgc atagtcatag cagtttactt
     3121 gttgaccatg aagtaaaaga agacttggtc cattttccgg accctttatg taataaattt
     3181 ccgaaccatc ttccaatttg gctacatttt ccgtaaaacc tgctttaaat ccggattttt
     3241 ctaaaaaatg ttcattcaga tagtcgtaag ttaaatttct gtatgcgaaa ataccggcag
     3301 caagtattac cgccatgaca attatcacta taaccaaaac gactctgttt ctcatacacg
     3361 gacgcgtttt ttacgttgac ttccgcgaac aatcaaccaa ataccaaagg ttacttccca
     3421 aagtccgccg agaccaagaa gatagatgct ccactctttc ccaaacaact ccatcaaaaa
     3481 gccgaaggca aaaacggcat aaccgattgc accccaaatg gccagccagg atggtgtgat
     3541 tttgtattta agcagtgtca agcacattgg aatactgccg atccccaaaa ccaacatggc
     3601 aagtttatat cccaggtcac ctaataggtt tcccttcaag aagatggcta agagcgctac
     3661 ggcttc
//
LOCUS       DAOOAI010000133         3636 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_28518_length_3636_cov_3.902820, whole genome shotgun sequence.
ACCESSION   DAOOAI010000133 DAOOAI010000000
VERSION     DAOOAI010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28518_length_3636_cov_3.902820"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..129)
                     /locus_tag="PK345_08175"
     CDS             complement(<1..129)
                     /locus_tag="PK345_08175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007662145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HPX35029.1"
                     /translation="MGTRKHKMAEERKLARKTLYIAKLSNCPTSPRKMRLVADLIRG"
     gene            complement(142..420)
                     /gene="rpsS"
                     /locus_tag="PK345_08180"
     CDS             complement(142..420)
                     /gene="rpsS"
                     /locus_tag="PK345_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007212071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HPX35030.1"
                     /translation="MSRSLKKGPYIYYKLEKKVLDAQESKKKSVIKTWSRASIISPDF
                     VGLTIAVHNGNKFIPVYVTENMVGHKLGEFAPTRQFRGHAGKKDKGRK"
     gene            complement(440..1264)
                     /gene="rplB"
                     /locus_tag="PK345_08185"
     CDS             complement(440..1264)
                     /gene="rplB"
                     /locus_tag="PK345_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HPX35031.1"
                     /translation="MALRKFKPTTSSQRFKLISAFDDITTDKPERSLLEPQKKSGGRN
                     NQGRMTMRYIGGGHKQKYRVIDFKRDKEGMAAEVLTVEYDPNRSARIALVLYSDGEKR
                     YILAPNGIKVGQTIMSGFGASPDIGNSLYLSEIPFGTLIHNIELRPGQGGKIARSAGS
                     YAQLMSRDGKYAILKFPSGETRMILQTCKATIGTISNQDHNQEISAKAGRSRWLGRRP
                     RTRGVVMNPVDHPMGGGEGKASGGIPRSRKGLPAKGYKTRYKKKASNKYIIERRKK"
     gene            complement(1270..1560)
                     /gene="rplW"
                     /locus_tag="PK345_08190"
     CDS             complement(1270..1560)
                     /gene="rplW"
                     /locus_tag="PK345_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005844851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HPX35032.1"
                     /translation="MDILIKPIITEKMTELGEKLNRYGFIVHKSANKLQIKKAVEDLY
                     GVKVDAVNTIINSGKRMSRNTKSGIIVGRTAKVKKAIVTVAKGEKIDFYSNI"
     gene            complement(1595..2224)
                     /gene="rplD"
                     /locus_tag="PK345_08195"
     CDS             complement(1595..2224)
                     /gene="rplD"
                     /locus_tag="PK345_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005853966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HPX35033.1"
                     /translation="MELTVYKINGEKTDRTVTLDDNIFGIVPNDHAIYLDVKQIMANR
                     RQGTHSTKQRSEVAGSTRKLKRQKGTGGARAGSIKNPIFRSGGRTFGPQPRVYRFKLN
                     KKLKRLARKSALSYKAESGNIMIVEDFNFETPKTKNFLAILKSLNVNETRVLMLTPNV
                     DRNIYLSTRNLGNITYLDAKSLNTYEVLKAKQLIILESSLNDIKEMFAN"
     gene            complement(2215..2844)
                     /gene="rplC"
                     /locus_tag="PK345_08200"
     CDS             complement(2215..2844)
                     /gene="rplC"
                     /locus_tag="PK345_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016275270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HPX35034.1"
                     /translation="MSGIIGKKIGMTGIFDENGKNIPCTIVEAGPCIVTQIKTKEKDG
                     YEALQLAYEDLKDKHVTKPIAGHFKKAGITPKRIVREFTRFEEGNRKKFGEKITVDIF
                     VEGEYIDVVGISKGKGFQGVVKRHNFRGSGDASHGQHNRLRAPGSIGASSSPSRVFKG
                     TCLPGQMGNKRVKMINLKIMKIIPEKNLLLIKGAVPGPNGSILIVERWS"
     gene            complement(2893..3198)
                     /gene="rpsJ"
                     /locus_tag="PK345_08205"
     CDS             complement(2893..3198)
                     /gene="rpsJ"
                     /locus_tag="PK345_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005782187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HPX35035.1"
                     /translation="MSQRIRIKLKSYDHNLVDKSAEKIVKTVKTTGAVVSGPIPLPTN
                     KKIFTVNRATFVNKKSREQFEVSSYKRLLDIYSSTAKTIDALMKLELPSGVEVEIKV"
     gene            complement(3255..>3636)
                     /gene="fusA"
                     /locus_tag="PK345_08210"
     CDS             complement(3255..>3636)
                     /gene="fusA"
                     /locus_tag="PK345_08210"
                     /inference="COORDINATES: protein motif:HMM:NF012882.2"
                     /note="EF-G; promotes GTP-dependent translocation of the
                     ribosome during translation; many organisms have multiple
                     copies of this gene; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HPX35036.1"
                     /translation="VVLKSGSFHQIDSDPLAFEIAAKIGFRNAARMMSLTLMEPVMRL
                     EVITPDQYLGEVSSDLNRRRGQIEDVSSKYNAQVLRAKVPLAEMFGYITQLRSLTQGR
                     ALYTLEFSHMPIYQPICSNRFCTK"
BASE COUNT          959 a          792 c          597 g         1288 t
ORIGIN      
        1 acctctgata aggtcggcaa caagtctcat tttacgaggc gatgtcgggc aattggacaa
       61 cttagcgatg taaagagtct tccttgccaa tttacgctct tctgccattt tatgttttcg
      121 tgtacccatg attattttgc tttatttcct tcctttatcc tttttacctg catgacctct
      181 aaactgacga gttggcgcaa actcacccag tttatgacct accatatttt cggttacata
      241 aacggggatg aatttattac cattatggac agcaatggtc aaacccacaa aatccggaga
      301 gatgattgat gctcttgacc aagttttaat cacactcttc ttctttgatt cctgtgcatc
      361 taaaaccttt ttctccaatt tataataaat atatggtcct ttttttaacg aacgactcat
      421 cttatcttca gtttaagaat tattttttac gtctttcaat aatatatttg tttgaagctt
      481 ttttcttgta tctggtttta taaccctttg caggcaatcc ttttctcgat cttgggatac
      541 cgccagatgc ttttccttcg cctccgccca ttgggtggtc aaccgggttc atcacaacgc
      601 ctctggttcg tggccttctt cctaaccaac gactacgtcc tgctttggcg gaaatctcct
      661 ggttatgatc ttgattcgaa atagttccaa tagtagcttt gcaagtttgc aatatcattc
      721 tcgtttctcc cgaagggaat ttaaggattg catatttacc gtctcttgac attagctgtg
      781 cataagaacc agcgctacgg gcaatttttc caccttgccc cggacgaagc tcaatgttat
      841 gaatgagtgt tccgaatgga atttcactca gataaaggga atttccaata tcgggcgaag
      901 cgccaaaacc agacattatg gtttgaccta ctttaatgcc gtttggagct aaaatatatc
      961 ttttttctcc atcggaatat agaactaatg caatgcgggc gctacgattt ggatcatatt
     1021 ccacagtcaa cacttcggca gccatgccct ctttatcacg cttaaaatca atgacacgat
     1081 atttctgttt atggcctccg ccaatatatc gcatagtcat cctaccctga ttattcctgc
     1141 ctcctgactt tttttgcggc tcaagtaagc ttctctctgg tttatcagta gtaatatcat
     1201 caaacgcact aataagttta aagcgctgac tggatgttgt cggtttaaat tttcttagag
     1261 ccatttatct taaatattgc tgtaaaaatc aattttttct cctttagcta cggtaacaat
     1321 agcctttttt acttttgcag tcctgccaac aattattcct gacttagtgt ttctcgacat
     1381 tcttttgcca gaatttataa ttgtgttaac agcatcaacc tttactccgt atagatcttc
     1441 aactgccttt ttaatctgca atttattggc gcttttatgc acaataaatc catatcggtt
     1501 aagcttttcg cccaactcgg tcatcttttc cgtaattatc ggtttaatta aaatatccat
     1561 tattattctc ctctgtttta gataattact tcatttaatt tgcaaacatc tcttttatgt
     1621 cattaagaga gctttcaagt attataagtt gtttagcctt taatacttca taagtattta
     1681 ggctttttgc atctaaatag gtaatattgc caaggttacg ggtggagaga tagatatttc
     1741 tatcaacgtt tggtgttagc atcaaaactc tcgtttcgtt aacattaaga gattttagga
     1801 tagcaaggaa gttttttgtc tttggagttt cgaaattaaa atcctcaact atcattatgt
     1861 ttccactctc tgccttataa ctcagcgctg attttcgcgc aagtcgtttc aactttttat
     1921 taagtttaaa acgatatacc cttggttgtg gcccaaaagt cctaccaccg cttctgaaaa
     1981 tcggattttt aatactgccg gcacgtgctc ctccggtacc tttctgtctt ttcaacttgc
     2041 gggtgctccc ggcaacctcg cttctttgtt tggtgctatg tgttccctga cgcctattgg
     2101 ccattatttg ctttacatcc aaatagatag cgtgatcatt cggaacaata ccaaagatgt
     2161 tatcatcaag ggttactgta cgatcggttt tttcaccatt tattttatat actgttaact
     2221 ccatctttct actattaaaa ttgatccatt aggtcctgga actgctcctt taattaataa
     2281 taaattcttt tctgggataa tcttcatgat tttcaggtta atcatcttga cacgcttgtt
     2341 acccatctga ccgggtaaac atgtaccttt gaaaacacgt gaaggcgacg aagaagctcc
     2401 tattgatcca ggcgccctca aacggttgtg ctgaccatga gatgcatcgc ccgaacccct
     2461 gaaattatgc ctttttacaa cgccctgaaa gcctttacct ttcgatatcc caacaacatc
     2521 tatatactct ccttcaacaa aaatatcaac ggtaattttt tcaccaaact ttttacggtt
     2581 tccttcttca aacctggtga attccctaac tattcgtttt ggggttatcc ctgctttttt
     2641 aaaatgacca gcaatcggct ttgttacatg tttatcctta agatcttcat aagcaagttg
     2701 aagagcttca tatccatctt tttcttttgt tttgatctgt gttacaatac atggcccagc
     2761 ctcgacgata gtacaaggaa tatttttccc attctcatcg aaaattccag tcattcctat
     2821 cttttttcca attattcctg acattatcct ttatgattta tgttgtcaaa tataatgaat
     2881 gctctctatt tatcaaacct tgatctcaac ttcaacacca cttggaagct caagtttcat
     2941 caatgcatca atggttttgg cagttgagct gtaaatatca agtagtcttt tgtaagaaga
     3001 gacctcaaac tgttctcttg attttttgtt tacaaaagtc gcacggttca ctgtaaagat
     3061 ctttttgttg gttggcaaag gtattggacc gctaacaacc gctcctgtag tctttactgt
     3121 tttaacaatc ttctcggctg atttatcaac caagttatga tcgtatgatt ttaatttaat
     3181 tcttatcctt tgactcactg taaattatga ttaaataatt ttatattatt tttaaacttt
     3241 aactatgtat ccttttattt tgtacaaaac ctgttcgagc agatcggttg gtaaatcggc
     3301 atatgcgaaa actcaagggt ataaagcgct cggccttgtg taagagaacg aagttgtgta
     3361 atataaccga acatttcggc caatggcact ttagctctta aaacctgagc gttatatttg
     3421 gatgagacgt cctcaatttg tcctctccga cgattcaaat cgctgctcac ctctcctaaa
     3481 tactggtcgg gggtaataac ctcaagccgc ataactggct ccatcaaagt aaggctcatc
     3541 atacgagcag cattacgaaa accaattttt gcagcaattt caaaggcaag cggatcggag
     3601 tcaatctggt gaaacgaacc gcttttcaat acaact
//
LOCUS       DAOOAI010000134         3613 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_28732_length_3613_cov_4.652333, whole genome shotgun sequence.
ACCESSION   DAOOAI010000134 DAOOAI010000000
VERSION     DAOOAI010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28732_length_3613_cov_4.652333"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1715
                     /locus_tag="PK345_08215"
     CDS             <1..1715
                     /locus_tag="PK345_08215"
                     /inference="COORDINATES: protein motif:HMM:NF024603.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HPX35037.1"
                     /translation="LIFVSISLFTITYLLSCANQLAPSGGPQDKEPPVVISSLPPNFS
                     THFDKNKIRLTFNEFVQLKQADEQILISPPVQKKPDYNLKGKSLFINLKEDLLPNTTY
                     SFFFGNSITDITEGNPLTNFLYVFSTGDYLDSLSIAGEVKNAFNLKPEEGVFVMLYPI
                     SNDTIPADSLPMKIKPSYVTKTDKEGNFRLNYLRNEPMKLFALKDINNNYLYDMPDEE
                     IAFTEEIVIPEHIKTMEPDTTLGQAPDSIHTKLLYDNFFHLKLFQQIDSTQRRLSETG
                     FYPPGFLITYRYGLKNPVINLFKEKNEAPWYIKELNKGRDSLRVWLTQPMPDSITIKI
                     QDGDFFSDTVLIVLPEKKTPETSKRRQKEETKQTEQLIINSNVKSRLLELNTPLELTF
                     EHPIKKYNKSHIWLFSEKDTVLANFSFADNANRKLKLIQKLEEETLYNLVIRDSTFFD
                     IYDHTHDSLVIVFNTRKTSDYGNLYLDVKIKNDSIPYILQLLDPKENVIHETVLNKSE
                     ELAFELLNPGIYTLKAIEDKHTNGVWDTGDYLKKREPEKVFFFQKELQIRTNWDIEEV
                     WKIE"
     gene            complement(1753..2052)
                     /locus_tag="PK345_08220"
     CDS             complement(1753..2052)
                     /locus_tag="PK345_08220"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HPX35038.1"
                     /translation="MKLYHINQEKLETAASKLKAISHPVRIAILELLSKEKMCVTDIY
                     EQLGLGQATTSHHLNILRTNGVLISKRHGKKKFYEIKHKALVDILDCINRCQDDN"
     gene            complement(2114..>3613)
                     /gene="ade"
                     /locus_tag="PK345_08225"
     CDS             complement(2114..>3613)
                     /gene="ade"
                     /locus_tag="PK345_08225"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HPX35039.1"
                     /translation="TPGLIDSHVHIESSMLNPSEFARLAVVHGTVATVSDPHEIANVL
                     GIEGVNYMIRNGNKVPFKFFFGAPSCVPATSFETSGATITSSDVSDLLKRDDVYYLSE
                     MMNFPGVLFNDHEVMAKIEAAKRFNKPIDGHAPGLMGNDIRKYIEAGISTDHECYTID
                     EALDKLKWGMKILIREGSAAKNFDELIDLIKDYPDKVLFCSDDKHPNDLVKGHINELI
                     KRAVGKGYDPLQVLRSASLNPIIHYNLDVGLLRPGDDADFIIVDNLADLNVLETYVKG
                     IQVAKNGRTLIESIEEETPNFFCAKKINESNIRVQTTKEKIKVIQVFDGQLITKEIVV
                     NPKVSDGSIVSNIENDILKLVVYNRYQPAQPSVAFVNSFNLKKGAIASTVAHDSHNII
                     ALGVNDSDIIRAVNLLIESRGGICFVDGAKEELLTLPVAGLMASEDGYKIAAAYEKLD
                     RLAKENGSTLASPFMTLSFMALLVIPELKLSDKGLFDSQKFEFTSLTCD"
BASE COUNT         1193 a          737 c          573 g         1110 t
ORIGIN      
        1 aacttatttt tgtttctatt tcgttattta caattactta tctgctctct tgtgccaacc
       61 agcttgctcc gagtggcgga ccacaggata aagagccgcc ggttgttata agttcgttac
      121 cacccaattt ttcaactcat ttcgacaaaa acaaaatcag acttaccttc aacgagtttg
      181 tacagctaaa acaagctgac gaacaaattc tcatctcccc tccagtacag aaaaagccag
      241 actataacct taaagggaaa tcacttttta ttaatttaaa agaagattta ttaccaaata
      301 ctacctattc cttttttttc ggcaattcca ttactgatat taccgagggg aatccgctaa
      361 ctaactttct atatgttttt tctacaggcg actacctcga ctcactatca atagccggcg
      421 aagtaaaaaa cgcttttaat ctaaagccag aagagggagt ctttgttatg ctctatccga
      481 tttcaaacga taccatccct gccgattcgc ttcccatgaa aattaaacca tcgtatgtta
      541 ccaaaactga taaagagggt aatttcaggt taaattatct tcgtaacgag ccgatgaaac
      601 tctttgcatt gaaagatatc aacaataatt atctttatga tatgcctgat gaagagattg
      661 cttttactga agaaatcgta atcccggaac atattaaaac gatggaacct gacactaccc
      721 tcggtcaagc gcctgattca attcatacta agctattata tgataatttt tttcatttga
      781 aactcttcca acaaattgat tcaacgcaac gcaggttatc ggaaacaggg ttttacccac
      841 ccggattttt gattacttat cgttatggac taaagaatcc cgtcattaat ctttttaaag
      901 aaaaaaacga agccccttgg tatattaagg agttaaataa aggaagggac tcacttagag
      961 tatggcttac acaaccaatg cctgatagca ttaccattaa aatacaggat ggagactttt
     1021 tttctgatac cgtactcatc gtattgcctg aaaagaaaac tcccgaaact tcaaaacgcc
     1081 gtcaaaagga agaaacaaaa caaacggagc aacttataat taatagtaat gtgaaatcaa
     1141 ggttgctcga attaaacaca cccttagagc ttacatttga gcatccgata aaaaaatata
     1201 ataaaagtca tatttggctt ttttctgaaa aagatactgt tttagcaaat ttcagttttg
     1261 cagataatgc caaccgaaaa ttgaagctaa ttcagaaatt ggaagaggaa acattgtata
     1321 acttggtcat ccgcgattca acatttttcg atatttatga tcatactcat gattctttgg
     1381 tgatagtttt caatacccgc aaaacaagtg attacggcaa cctatactta gatgtaaaga
     1441 ttaaaaatga ttcaattcca tatatcctgc aactgcttga tccgaaagaa aacgtcattc
     1501 atgaaacagt acttaacaag tcagaagaat tggctttcga attgttaaac cctggtattt
     1561 ataccctcaa agctattgag gacaagcata ctaacggagt atgggatacg ggtgattact
     1621 taaaaaagag agaacccgaa aaggtttttt tctttcaaaa agagctacag atacgaacca
     1681 attgggacat tgaggaggta tggaaaatcg agtaaagtaa agcctaaaag agtaacattt
     1741 gtgttacaac aatcagttat catcttgaca tcggtttata caatcaagaa tatccacaag
     1801 agctttgtgt ttaatttcat aaaacttttt cttcccgtgc cgtttactga ttaaaacacc
     1861 atttgttctt aaaatattta gatgatgtga tgtggtagcc tgccctaaac caagttgttc
     1921 ataaatatca gttacgcaca ttttttcttt actcaataat tcaaggatgg caatccttac
     1981 aggatgagag atagccttta gctttgaggc ggcagtttca agtttttctt gatttatatg
     2041 atataatttc ataggtattt ttccgctaaa atactacaaa aaacaatatc tgcaaattga
     2101 tttacatcaa ttctcaatca caagtcaacg aagtgaactc aaatttctgt gaatcaaaaa
     2161 gccccttatc actaagttta agttcaggaa ttaccaaaag cgccataaaa gaaagtgtca
     2221 taaaaggcga agccaatgtt gatccatttt ctttagctaa ccgatctaac ttctcgtagg
     2281 ctgcagcaat cttgtatcca tcctccgacg ccatgagtcc tgcaaccggt aatgtaagta
     2341 actcttcctt agcgccatcc acaaaacaaa taccacctcg ggattcaatt aacagattga
     2401 ctgccctgat aatatccgaa tcattaaccc ccagcgctat aatattatga ctgtcatgcg
     2461 ctactgtaga agctattgct ccttttttca ggttaaagct attaacaaaa gcaaccgaag
     2521 gttgtgcagg ctgataacga ttgtaaacca caagctttaa aatatcattt tcaatattac
     2581 taactattga accatcagaa actttcggat ttacgactat ctcttttgtg atcagttggc
     2641 catcaaatac ctgaatgact tttatttttt cttttgtggt ttgtactctt atgttactct
     2701 cattaatctt tttagcacag aaaaaattag gggtctcctc ttcgattgat tctatcaagg
     2761 ttcttccatt tttagctact tgaattcctt tcacgtaagt ctctaagaca ttaagatcag
     2821 caagattatc tactatgata aaatcagcat catcgcccgg tctaagtaaa ccaacatcta
     2881 aattataatg tattattgga tttaaagaag cgctgcgcaa tacttgtaaa ggatcgtaac
     2941 ctttcccaac tgcacgtttt ataagctcgt ttatatgtcc ttttacaaga tcattaggat
     3001 gtttgtcgtc ggaacaaaac aatactttat ccggataatc cttaataaga tcaataagct
     3061 catcgaagtt tttcgccgca cttccttccc tgatcagaat tttcatgccc cattttaatt
     3121 tatccagcgc ctcatcaata gtatagcatt catgatcggt agatatgccc gcctctatat
     3181 atttacggat atcattaccc attaagcctg gcgcatgacc gtcaataggc ttattaaatc
     3241 tcttcgctgc ctcaatctta gccattacct cgtgatcgtt aaaaagaaca cccggaaaat
     3301 tcatcatttc cgacaaataa taaacatcat ctctttttaa caaatctgaa acatcgctgg
     3361 atgtaatagt agctccagaa gtttcaaatg atgtagccgg aacacatgaa ggagccccaa
     3421 aaaagaattt aaaaggtacc ttattcccat tccttatcat ataatttacc ccttcaatgc
     3481 ccaacacatt tgcaatttca tgaggatcag agactgtagc aacggtacca tgaaccacag
     3541 caagtcgtgc aaattccgag gggttaagca tactgctttc aatatgaaca tgtgaatcta
     3601 ttagcccggg agt
//
LOCUS       DAOOAI010000135         3512 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_29750_length_3512_cov_5.842349, whole genome shotgun sequence.
ACCESSION   DAOOAI010000135 DAOOAI010000000
VERSION     DAOOAI010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3512)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3512
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29750_length_3512_cov_5.842349"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(7..1530)
                     /locus_tag="PK345_08230"
     CDS             complement(7..1530)
                     /locus_tag="PK345_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008155270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HPX35040.1"
                     /translation="MQKSIRLFFSSFFLLFSIALYSQTDSIVRRIIDIGNSDNQTMHH
                     QDVLCNRFGGRLVGSAAFTDAANWAAHKFKEWGMEVIMDEAGEVPVGFNRGPWFGRLL
                     GDNPMTLHFATPSYTAGTKGVLKGHVVLEPKTRAQFDRMKGKLKGAWVLVTGKSKGWP
                     ISYSDKAAQKRDSIINLNEEIEVKNRKIRYENWQNRDKGVPEKEQLPYNEEPGLFYKE
                     MVEAGILGIIQSAPIPITALYDRENLHDMEFETLPTVADIKLNEHQFAVIEQMAKERQ
                     RFELEFDIRNHFSMGPVKYHNVIGIIPGTEYPDEYVILSAHLDSYDVATGAVDNSSGV
                     GPTMEAARLIMEAGGKPKRTILVILWAAEEFGLYGSTSWLERNADKHDKISNMFNRDG
                     APTVANSISVPPILMEDFEKICEPLNDINPDFPFTLRQGEPMRRPTEPWSTDSGPFAV
                     AGIPVVSFGTTDPKGYDFDYNEVWHTERDLYNMIIPEYQEHTSIVTAIVTAIRELVK"
     gene            2036..>3512
                     /locus_tag="PK345_08235"
     CDS             2036..>3512
                     /locus_tag="PK345_08235"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid ammonia-lyase"
                     /protein_id="HPX35041.1"
                     /translation="MSLIITGSNLTIDDVVKVARHHEKVELHPDAIERIKRCRAMLEK
                     KIVAREIMYGVNTGIGEFSEVVLTDDQVKDFQKYLIYNHAAGIGEPMAEEYVRGAMLG
                     RINVHAHGNSGIRPEITLTLVEMLNKGVTPWVCKKGSVGACGDLAPMSQIALLMMGEG
                     KAYYQGELLSGKEALERAGIEIPGLKARDGLGTINGSNVLTAMSAIFLYDANRWLKQA
                     EIGAAMSLEALKANMRPYAAKLHEVRGFKGAIRSAEAIRKIVAGGDLAEGRVKCKVQD
                     AYSMRSTPQVIGAAHDALAYARSQVEIELNGVGDNPIFFPDENIQLSGANFQGTPVSL
                     PMDMAGIAVTMVSVMSERRMNRLNNPALSVGLPAFLTKGAGMFSGLMLSQYTADMQIV
                     EQRILSAPASIQSIPAAADQEDFVSMGMNTAIKNFQILDNAYGVLGIEIMAAAQALDF
                     REYKFGQGTSKAKEIVRKYVEFLDVDRPLYDDHNAMKKLVKS"
BASE COUNT         1023 a          705 c          760 g         1024 t
ORIGIN      
        1 ttttgctcat ttcaccaact ctcgaatggc tgtaacaata gctgtaacaa tagaagtgtg
       61 ttcctgatat tcgggtatta tcatattgta tagatcgcgt tcggtatgcc atacctcatt
      121 gtagtcgaag tcataacctt tgggatcggt agttccaaag gacactaccg gtattccggc
      181 tacggcaaaa ggaccgctgt cggtactcca gggttcggta gggcgtctca ttggttctcc
      241 ttggcgcagg gtaaaaggaa aatcaggatt aatatcattc aggggttcac atattttttc
      301 aaaatcctcc attaatattg gtggcacgct gatgctattg gcaacagttg gagcgccatc
      361 acggttgaac atatttgata ttttgtcgtg tttatcagca ttacgctcaa gccagcttgt
      421 cgaaccatat aatccaaact cctctgctgc ccaaaggata acaaggattg tccgtttcgg
      481 ctttccaccg gcctccataa ttaggcgtgc agcctccatt gttgggccta cgcccgaact
      541 attatcaaca gctcccgtgg ctacatcgta agaatcaaga tgagcgctga ggatgacata
      601 ctcgtcagga tattccgtcc cgggtattat accgataaca ttatgatatt tcacaggccc
      661 catgctgaag tgattccgta tatcaaactc caattcaaag cgttgtcgct cttttgccat
      721 ttgctcaata acagcaaatt gatgctcatt aagtttgatg tcagcaactg ttggcaaggt
      781 ttcaaattcc atatcatgta agttctcacg gtcgtataaa gctgttattg gaataggagc
      841 tgattgaata atacccaata ttccagcctc taccatttcc ttgtaaaaca gaccaggttc
      901 ttcattatat ggtaattgct ctttctcagg cactccttta tctctgtttt gccagttctc
      961 ataccggatt ttcctgtttt tcacttcaat ttcctcattc aggtttatta tcgaatcccg
     1021 tttttgtgca gctttatcag aataacttat aggccatcct ttgcttttac ctgttaccaa
     1081 aacccaagcc cctttcagct tccctttcat acggtcaaat tgggctctgg tctttggctc
     1141 aagtacaaca tgccctttca gcacgccttt agtacccgcc gtataggaag gagtagcaaa
     1201 atgaagtgtc ataggattgt cgcccaacaa acgaccgaac cacggcccgc gattaaagcc
     1261 aaccggtact tcgccggcct cgtccataat aacctccata ccccattctt taaacttatg
     1321 agctgcccag ttagcagcat cagtaaaagc agcagagcca accaaccggc caccaaaacg
     1381 attgcaaagc acatcctgat gatgcatagt ctgattgtca gaattaccaa tatctataat
     1441 acgtctgact atactatcag tctgagagta aagagcaata gaaaacaaca aaaaaaatga
     1501 agagaaaaaa agtctgatag atttttgcat agtttaatgt ttttcaatga gatctcttcg
     1561 aaagaagaac gctgacaatg ggcaaaggta aataaactcc aactaatcaa cagaaaggtt
     1621 tttttgggtt gaatatttta aaggttactt tttttctaaa cctgtgataa aattatcaaa
     1681 acacatgtca aggtattttt gaggttgaag aaaagtaaga atggtaaatt tttgtaaatg
     1741 caaaacgttt ttcatggtac aaaatattaa aagtaacaat ttttctgtcc ggttattttc
     1801 tgactaattc agcttgatct attattattc ataaaaaaag aaaaagggct gtttagattg
     1861 tatatttttg tgtttctttg atgttttaaa atttgagccc gctctaaaaa tgattttttt
     1921 cagatattcg tgtattgtta ccgaacaaag ttggagtatt cgtggctaaa attactttta
     1981 ggagcaaact catatttcaa atttaaacta ttctttaata aaaatctaaa attgtatgtc
     2041 acttatcatt accggatcta atctaaccat tgatgatgtt gtaaaagtag ctcgtcatca
     2101 tgaaaaagtt gaacttcatc ctgatgccat tgagcgtatc aaaagatgtc gtgcaatgct
     2161 ggaaaagaaa attgtcgccc gcgaaataat gtatggtgta aacactggta ttggcgaatt
     2221 ttcggaggtt gtacttactg acgatcaagt aaaagatttt cagaagtatc tcatttacaa
     2281 tcatgctgct ggtattggcg agcctatggc agaggagtat gtaaggggcg ccatgttagg
     2341 ccgaataaac gtacatgctc acggcaactc aggtatcagg cccgaaatta cattgaccct
     2401 tgtagagatg ctcaataaag gcgttacccc atgggtctgc aaaaaaggtt ctgttggcgc
     2461 ttgtggagat ttggctccaa tgtcgcaaat tgctttgttg atgatgggcg agggtaaggc
     2521 ttattatcag ggcgagttgt taagtggaaa agaggcatta gagagggcag gtatcgagat
     2581 accagggctt aaggcccgtg atgggttagg tactatcaac ggatcaaacg tgcttacagc
     2641 aatgagcgct attttcctat atgatgccaa tcgctggttg aaacaggctg agataggcgc
     2701 tgctatgtcg ttggaagctc ttaaagccaa tatgcggcct tacgcagcca agttgcacga
     2761 ggtgagaggt tttaaagggg caatcaggag cgctgaagcg atcagaaaaa tcgttgcagg
     2821 tggcgatctt gccgaaggtc gtgtaaaatg taaagtacag gatgcttact cgatgcgttc
     2881 aacgccacag gtaattggtg cagcacatga cgcattggct tacgctcgct cgcaagtgga
     2941 gatagagcta aatggagtag gcgataatcc aatctttttc cctgatgaaa atattcagct
     3001 ctcaggcgct aattttcaag ggacgcccgt ttcgttgcct atggatatgg ctgggatagc
     3061 ggttactatg gtaagtgtaa tgtcggagcg gagaatgaac agactgaaca atccggcatt
     3121 aagtgtgggg cttccggcat ttctaaccaa aggtgcaggc atgttttcag ggttaatgtt
     3181 aagccagtac accgccgaca tgcaaattgt agaacaacga atacttagtg cgccagcctc
     3241 catacaatcc atcccagcag ccgctgatca ggaagacttt gtttcgatgg gaatgaatac
     3301 agcaataaaa aatttccaaa ttctcgacaa tgcttacggt gttttgggaa ttgagataat
     3361 ggctgcagct caggctctcg actttagaga atataagttc gggcagggaa cctctaaggc
     3421 taaagagatc gtaagaaagt atgttgaatt tcttgatgtg gatcgcccgc tgtacgatga
     3481 tcataacgca atgaaaaaac ttgttaaatc tt
//
LOCUS       DAOOAI010000136         3494 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_29924_length_3494_cov_5.198313, whole genome shotgun sequence.
ACCESSION   DAOOAI010000136 DAOOAI010000000
VERSION     DAOOAI010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29924_length_3494_cov_5.198313"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="PK345_08240"
     CDS             <1..292
                     /locus_tag="PK345_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX35042.1"
                     /translation="FDLLRQFGELKIGFFATVTKSSDFNGGFNFSIPLPPGKYMRIKY
                     LRVRQSKDFSLEYRAKGFSKSVGTYDTGSQLYNILMEYNADFFKKRLIKELF"
     gene            368..1516
                     /locus_tag="PK345_08245"
     CDS             368..1516
                     /locus_tag="PK345_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX35043.1"
                     /translation="MKIKNYTRYLVLPLLALAMVFAFNQCKNVGQTDWPDPDVAILNQ
                     LKINVINTNDGLSLEGYDIKVVLPNGTTKEFNGNTGTFTFDGTTEGTYVITASKEGYL
                     AESTVINVSNDQTEAVSTVTQHVFFLNKKGSTNVVSQQGTVLYVESDQSTPTTITFPA
                     GSLPSDQNITVTYIAPLAQFEELNILGERAILYGYNFSPDFTFPDNARPTITIPINIP
                     SVTDGETDLWIGTYNENTNTWEAYLGTLNAERTTATFEMPHFSTWFVFTGYKLIKDVE
                     IWSPWSFVAESEKCGDGVCGTFIYAVTPNALVNRLLSVGYNFNLKVKDTRCIGPHAKY
                     IQQLFARAQLITYKVYDYQGVYLGAIQVPTKKVQWMADEFYCHDQGDI"
     gene            1623..2300
                     /locus_tag="PK345_08250"
     CDS             1623..2300
                     /locus_tag="PK345_08250"
                     /inference="COORDINATES: protein motif:HMM:NF024895.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HPX35044.1"
                     /translation="MKRTIVFFIVFFFMSLNGFSQEKSQLRFNSLGLKIGWYNPSLDY
                     WKNESEFKNADFNGGFCYNVFGDLSIIKNALGKWNMGVWQSSVEDNIQGFGLTTWTLT
                     GMTASFDLTYQAKQITFYRITPYIGAGGEFIYLEQKLKFENKETPSPVNGSTLIAKGL
                     AGFEFKFSEQWAMDLEFNYKIGNYKQDFKHYEANPNDPENPLEKEITEKISLNGPMIS
                     IGLKYLF"
     gene            complement(2316..>3127)
                     /locus_tag="PK345_08255"
     CDS             complement(2316..>3127)
                     /locus_tag="PK345_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019245836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HPX35045.1"
                     /translation="HGIHGRAPAIASGVKMANPDLSVWVITGDGDALAIGGNHFIHVI
                     RRNINLNIILLNNQIYGLTKGQYSPTSFQGQISKTSPFGTIEPPFHPGELTLGAQGNF
                     FARSLDYNVKLTTDILVEAAKFQGTSVVEVLQNCVIYNDNAYGYINDKAYKEDRQLIL
                     EQGKPMIFGKNNDKGIIMEDMQLKVVKLGENGITEKNILIHDAKAQSPLIHWMLAKMN
                     FPEFPVAFGVIRAVEEDTYSLKMKRQIEYIQKNSKIKNVDDLLKSGNTWEV"
     assembly_gap    3128..3137
                     /estimated_length=10
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3138..3365)
                     /locus_tag="PK345_08260"
     CDS             complement(<3138..3365)
                     /locus_tag="PK345_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015545768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HPX35046.1"
                     /translation="MEEIKQYTPKDFKSDQEVRWCAGCGDHAVLSAVHRAMAQTGIPR
                     EKIALISGIGCSSRFPYYMNTYGMHSIHGRAT"
BASE COUNT         1077 a          639 c          639 g         1129 t
ORIGIN      
        1 atttgattta cttcggcagt ttggcgagct gaaaattggt ttttttgcta cggtaaccaa
       61 gtcctctgat tttaatggtg gatttaattt ttccattccc cttcctcctg gtaaatacat
      121 gagaataaaa tatttaagag tacgccaatc gaaagatttt agtttggaat accgggcaaa
      181 aggttttagt aaaagtgttg gaacatacga tactggtagc cagctttaca atattttgat
      241 ggagtataat gcagattttt ttaaaaaaag attaataaaa gaattgtttt aataaaaatt
      301 atttattttt gcaagttgta ttaaaaacga atttaataat gataaacaat tataaaaagg
      361 agtaaagatg aaaatcaaaa actacacacg ctatttagtc cttccattgc ttgctttagc
      421 aatggtgttt gcatttaatc aatgtaaaaa tgtgggacaa actgattggc cagatccaga
      481 cgttgcaata ctcaatcagt taaagatcaa tgtaataaat actaatgacg gactttctct
      541 tgagggttac gacattaagg ttgtacttcc taacggaacc actaaagaat ttaacggtaa
      601 taccggaaca ttcacttttg atggtacaac cgaaggaacc tatgtaatta ctgcttccaa
      661 agagggatac cttgctgaaa gcactgtaat taacgtaagt aacgatcaga ctgaagctgt
      721 ttcgacagta acgcaacatg tgtttttcct taataaaaaa ggaagcacta atgttgtttc
      781 ccaacagggc acagttcttt atgtcgaaag cgatcagtct accccaacaa ctattacttt
      841 tccggcaggc tccttacctt ccgatcagaa catcaccgtt acttatatcg cgccattagc
      901 acaatttgaa gagctaaaca ttttaggtga aagggcaatc ctttatggtt ataatttctc
      961 acccgacttc acctttcctg ataatgctcg tcctaccatt accataccta ttaatattcc
     1021 aagtgttacc gatggtgaga ctgacctatg gataggaact tacaacgaaa acaccaatac
     1081 atgggaagct taccttggaa cactgaatgc agaacgaaca acagccactt ttgagatgcc
     1141 ccatttttct acctggtttg tttttacagg atataagtta ataaaagatg tagaaatctg
     1201 gagcccatgg tcatttgttg ctgaatcaga aaagtgtggt gatggtgtct gtggtacatt
     1261 catctatgcg gtaaccccca atgcattggt taatcgctta ttatccgtag gttataattt
     1321 caatctaaaa gtaaaagata cgagatgtat tgggccacat gctaaatata tacaacaact
     1381 tttcgctcgc gcacaactga ttacttataa ggtttatgat tatcaaggtg tctatcttgg
     1441 agcgattcaa gtacctacca aaaaggtaca gtggatggca gatgaattct attgccacga
     1501 tcagggagat atataatcta aatccagggt ttttttaatc atttaatatt attagcatga
     1561 agcttgaatt tattgagctt catgcttttt tttaaatcaa aacctacttg aactgaagct
     1621 ttatgaagcg tacaattgtt ttttttattg ttttcttttt tatgtcatta aatggttttt
     1681 cacaagaaaa atcacagtta aggttcaata gccttgggct aaagattggt tggtataatc
     1741 cttcattaga ttactggaaa aatgaatcag agttcaaaaa tgctgatttt aacggagggt
     1801 tttgctacaa cgtttttggt gatttatcca ttattaaaaa cgcacttggg aaatggaaca
     1861 tgggtgtttg gcaatccagc gtcgaagata atattcaggg atttggcctc accacttgga
     1921 ctttaacggg gatgacagcc tcttttgatt taacttatca ggcaaagcaa attacatttt
     1981 acaggataac accatacatt ggagcaggcg gagaatttat ttatcttgaa caaaaactta
     2041 aatttgaaaa taaagagact ccttctcctg ttaatggttc aactttaatt gccaaaggct
     2101 tagccggttt tgaatttaaa ttttcagaac aatgggcaat ggatttagaa tttaattata
     2161 agataggtaa ttacaaacag gattttaaac attacgaagc aaaccccaac gaccctgaaa
     2221 atcctttaga aaaagaaata actgagaaaa tttcacttaa tggacctatg ataagtattg
     2281 gtttgaaata ccttttttaa tcccgtttgt tggtattaca cctcccatgt atttccactt
     2341 ttcaaaaggt catccacatt tttaattttc gaatttttct gaatgtattc tatctgccgc
     2401 ttcatcttca aactataagt atcttcttca accgccctga ttacgccaaa ggccaccggg
     2461 aattccggga agttcatttt tgcaagcatc cagtggatta aaggactttg tgccttagca
     2521 tcatgtataa gaatattctt ttcggtaatt ccattttcac caagttttac cactttcaac
     2581 tgcatatctt ccattattat ccctttatca ttatttttcc caaaaatcat tggcttgcct
     2641 tgctctaaaa tcaactggcg gtcttctttg taagccttgt cgtttatata accgtaggcg
     2701 ttgtcgttgt aaattacgca attctgaagt acttcaacta ctgacgtgcc ttggaactta
     2761 gcagcttcaa caagaatatc ggtggtaagt tttacattat aatcaagtga tcgagcaaaa
     2821 aagttaccct gggctcctaa tgtaagctcc cctgggtgaa atggaggttc aatagtgcca
     2881 aaaggtgatg ttttgcttat ctgcccttga aacgatgtcg gagaatattg tcctttggtt
     2941 aggccataaa tttggttatt taaaagaata atattcagat ttatgtttct tcgtattaca
     3001 tggataaaat gattccctcc aatagctaag gcatcgccat cgcctgttat cacccatacg
     3061 ctaagatcgg ggttagccat ttttactcct gatgcaatag ctggtgcacg tccatggatt
     3121 ccatgaannn nnnnnnntgt tgcacggccg tgaatggagt gcatgccata ggtgttcatg
     3181 taataaggaa atctagaaga gcaaccgatt cctgatatta acgcaatttt ttcgcgtggg
     3241 atacctgtct gtgccatagc acgatgcacg gctgatagca cagcgtgatc gccacaaccg
     3301 gcgcaccacc taacttcctg atcactttta aaatctttgg gggtatattg ttttatttct
     3361 tccatagcta taaattattt cagaattaat tcaaaatgtt ctacaagttc tgccatagta
     3421 aatggttgac cctgaacttt gttgtgttga aggaaattca ctcccggcac tttggagcgc
     3481 aggaatgaag caaa
//
LOCUS       DAOOAI010000137         3461 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_30312_length_3461_cov_4.427775, whole genome shotgun sequence.
ACCESSION   DAOOAI010000137 DAOOAI010000000
VERSION     DAOOAI010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3461)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3461
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_30312_length_3461_cov_4.427775"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(227..1108)
                     /locus_tag="PK345_08265"
     CDS             complement(227..1108)
                     /locus_tag="PK345_08265"
                     /inference="COORDINATES: protein motif:HMM:NF023385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HPX35047.1"
                     /translation="MKSKSSVEHNDVNFLTFDVEEWYAVNYKSVNLSLYNDSGTHLEK
                     NMDKLLEICAENKIKSTCFIVGDIARNKPNIIKKIHSEGHEVASHSYNHKMINKMTPD
                     QFRLDLHKSCDILEQIIGEQIIGYRAPSWSVNQEILPWFYNILNEREIKYSSSVFPGK
                     TFLYGIKNFPQEIHQPLIDGVKQPVWEIPQVLFSVIGKKYGFSGGFYLRALPLFFVKK
                     LIVKKNRKGKNIFVYLHPREIDPKSPKLKLPLIEKLIHYYGVKGCEFKLRSILKNFQP
                     TFIRMGDYINQLNQSEK"
     gene            complement(1118..1486)
                     /locus_tag="PK345_08270"
     CDS             complement(1118..1486)
                     /locus_tag="PK345_08270"
                     /inference="COORDINATES: protein motif:HMM:NF022106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX35048.1"
                     /translation="MLKVVLLSISTTLLITTGQVLWKIGIQKAGGFHLPGEPLFANIF
                     RIVFNGWVFSGFMVYALATVFFMWLISKFEISLIVPITSVAFIYSLLAGYFIFHEQIS
                     IVRIAGVLLIILGVFLVVKN"
     gene            complement(1480..2430)
                     /locus_tag="PK345_08275"
     CDS             complement(1480..2430)
                     /locus_tag="PK345_08275"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HPX35049.1"
                     /translation="MFENKKLAVVIPTFKAAAHLKDVINGLPPIVDYIIVVDDCCPQK
                     SGEIAVEMQKTDSRILTIWHQQNKGVGGATISGYKKALELDCDIIVKMDSDDQMDPAY
                     LPDLIRPVVDGKAGYSKGNRFVDFKALRSMPGFRLFGNSILSFLVKACSGYWNMMDPT
                     NGYTAIKKETLQKINLDKISNRYFFETDMLIRLNIQNTVVKDIPIPARYGDEKSNLSI
                     RNTIFRFPYLLVKGLIKRFILKYLIYDFNMASVYVLTGLPMLLWGLFYGLFKWLENTI
                     NGVATPTGTIMLSVLPLILGTQFLIAAINIDINSTPKNTC"
     gene            complement(2471..>3461)
                     /locus_tag="PK345_08280"
     CDS             complement(2471..>3461)
                     /locus_tag="PK345_08280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX35050.1"
                     /translation="LVIGLVYLRRLLLNFFSEWVVAIVLLIIPLATNMLWYTVVESAM
                     SHVYNFALISVFLYHTNKWHEQITLKRSVYIGLLSGLITLIRPTNIIVLIIFALWKTG
                     NWNDIKKRLILFLQKWHLVLIMIISFVLVWTPQMLYWKLQTGHYFYYSYPDDQGFFFA
                     NPQIWNTLFSWRKGWLLYTPIMIFALTGIVVLRKSYPKLFIPILTFTIINIYVISSWW
                     DWWYGGGFGLRAYIDMYGVMAIPLAAMLTWISERKRVTKYILIFIFALTTAKSIHHHF
                     QYHYGSIHWVSMTKEAYIDSFWRISPSKNFQHLLKNPDYNLARKGIYKYEGEN"
BASE COUNT         1118 a          616 c          651 g         1076 t
ORIGIN      
        1 taccatacat ctatagggtg gaaattccga acgtgccagc caattggaaa cgttggtcaa
       61 agacaaaggt gttgcttgtg agagcaaatc tgaaggaagt cggcaacaaa accgtgcatg
      121 aacgaacata aacacaatac aaggcttgcc ttgtgggtaa gtaggcaatg gactgcgaag
      181 ccctataatt ggacgtaaaa caagggtagt aaattacatc aactctttat ttctcagact
      241 gatttaattg atttatgtaa tctcccatac ggataaaggt aggctgaaaa ttttttagaa
      301 tactacgtaa tttgaattca caccctttaa caccataata atgaatcagt ttttcgatca
      361 atggaagctt taatttaggc gacttggggt caatttctct cggatgaaga tagacaaaga
      421 tattttttcc tttccggttt tttttaacaa ttaatttttt aacaaaaaac agtggtaatg
      481 cacgaagata aaatcctccg ctaaaaccat atttttttcc tataactgaa aacagaacct
      541 gcggaatttc ccaaaccggc tgtttaaccc cgtcaattag aggctgatga atttcctgtg
      601 gaaaattttt gattccataa aggaaagttt ttcctgggaa aacagaagag gagtatttta
      661 tttctcgctc attaagaata ttataaaacc aaggcagaat ttcttggttt actgaccaag
      721 agggggctct gtaaccaata atctgctcgc cgattatttg ttctaaaata tcgcaggatt
      781 tatgaagatc aagtctgaac tggtcaggtg tcattttatt tatcattttg tgattatagc
      841 tgtgggaagc tacttcatgc ccttcactat gtattttttt tattatgttt ggtttattcc
      901 gagctatatc accaacaata aaacaagttg attttatttt attttctgca caaatttcaa
      961 ggagcttgtc catatttttt tcgagatggg tacccgaatc attataaaga cttaggttta
     1021 cggatttata gtttaccgca taccactcct caacgtcaaa agtgagaaaa ttgacatcgt
     1081 tatgttcaac actactttta cttttcatga tttaggttta atttttaacc acaagaaata
     1141 cccctaaaat gatcagcaga acgccggcta tacgcacgat cgagatttgc tcgtgaaaaa
     1201 tgaaataacc ggcaagtaaa gagtatatga atgccacact tgtaataggt acaatcaggc
     1261 tgatttcaaa tttggaaatc agccacatga aaaaaactgt agcaagggca taaaccataa
     1321 accctgaaaa tacccatcca ttgaagacaa ttcgaaagat gtttgcaaac agaggctccc
     1381 caggtaggtg aaatccacct gctttttgaa ttcctatttt ccaaagcact tgtcctgtgg
     1441 tgataagcag tgtggttgat atggataata aaactacttt taacatgtat ttttgggtgt
     1501 tgagttaata tctatgttga tagcggctat taagaactgc gttccaagta ttagcgggag
     1561 taccgaaagc atgatagtac ctgtaggagt agctacaccg ttgattgtat tttccaacca
     1621 cttaaacaat ccgtaaaaga gcccccagag caacatcggt agtccggtaa gcacgtaaac
     1681 cgaggccata ttaaaatcat aaatcagata tttcagaata aaacgcttga ttaatccttt
     1741 aactaaaaga taaggaaacc tgaatatcgt gtttctgata cttaaattac ttttttcatc
     1801 tccatatcga gcagggattg ggatatcttt tactactgta ttttggatat taagcctaat
     1861 gagcatatct gtttcaaaga agtaacggtt tgagattttg tcaagattta tcttttgcaa
     1921 agtctctttt tttatggcgg tataaccatt agttgggtcc atcatattcc agtaccctga
     1981 gcacgctttt accaaaaatg agagaatgct gttgccaaaa agtctgaagc cgggcatgga
     2041 gcgtaatgct ttaaaatcaa caaatctgtt tcctttggaa tagccggctt ttccatctac
     2101 cacagggcgt atcaagtcgg gtaggtaagc agggtccatt tgatcgtcgc tatccatttt
     2161 aactataatg tcgcagtcaa gttcaagcgc ttttttgtaa ccactaatag ttgctccccc
     2221 aacaccttta ttttgttgat gccaaatagt taaaatacgt gagtcggttt tttgcatttc
     2281 tacagcaatt tcacccgact tttgagggca acaatcatcc acaacaataa tataatcaac
     2341 tattggtggg aggccgttga tcacatcttt taaatgtgcg gcagccttaa aggtgggtat
     2401 gacaacagct aattttttat tttcaaacat taggtatagt tgttctattg atgattagta
     2461 aattgtaagt ttaattttct ccttcgtatt tatatatgcc tttgcgtgca agattgtaat
     2521 caggattttt caaaaggtgt tggaaatttt tcgatgggct gattcgccag aaagaatcaa
     2581 tataggcctc tttggtcatc gaaacccaat gaattgaacc gtaatgatat tgaaagtgat
     2641 gatgaatact tttagcagtg gtaagggcaa aaatgaaaat caaaatatac tttgtaactc
     2701 tttttctctc tgaaatccag gttagcatag ccgctaaagg tatagccatt acgccgtaca
     2761 tatcaatata ggctcttaat ccgaatccgc ctccatacca ccaatcccac cacgaagaga
     2821 taacgtaaat attgataatg gtaaatgtaa gtataggaat gaaaagcttc gggtaacttt
     2881 ttcgtaaaac tacaattcct gtcagtgcaa aaatcatgat aggagtatag agtagccagc
     2941 ctttgcgcca tgaaaaaaga gtattccaaa tttggggatt agcaaaaaag aacccttggt
     3001 cgtcaggata tgaataataa aaataatgcc ctgtctggag cttccaatag agcatttgag
     3061 gtgtccaaac cagtacaaaa ctgataatca tgattaacac taaatgccat ttttgtaaaa
     3121 aaagaatcaa tctttttttg atgtcattcc agttgccggt tttccaaaga gcaaagatta
     3181 taagaactat aatattagtt ggtcgaatta gggtaattag tccggatagt agtccgatgt
     3241 aaacggaacg ttttaatgtg atttgttcat gccatttgtt ggtgtgatat aggaaaactg
     3301 aaataagtgc aaagttgtaa acatgcgaca ttgccgattc tactactgtg taccatagca
     3361 tatttgttgc caatggaatg attagtaaca caatggctac aacccattcg gaaaaaaagt
     3421 ttagcaatag cctgcgtaga taaaccaatc ctattacaag g
//
LOCUS       DAOOAI010000138         3380 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_31254_length_3380_cov_6.232782, whole genome shotgun sequence.
ACCESSION   DAOOAI010000138 DAOOAI010000000
VERSION     DAOOAI010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3380)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3380
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31254_length_3380_cov_6.232782"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..487)
                     /locus_tag="PK345_08285"
     CDS             complement(<1..487)
                     /locus_tag="PK345_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009136142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HPX35051.1"
                     /translation="MNEEGKSILEKSLRTVSDGRSVGARTLTSALMEYGKVPPQAIDL
                     EEAVIGGLLLEQNALTAVIDFLKPEVFYLDAHKKIYSAIQDLFARQKPIDMLTVTHEL
                     KSKGELEIVGGAYYIAQLTRRVASAANIEYHARIVVQKYIQRRLIEIASEITKEAYED
                     TT"
     gene            complement(1009..>3380)
                     /locus_tag="PK345_08290"
     CDS             complement(1009..>3380)
                     /locus_tag="PK345_08290"
                     /inference="COORDINATES: protein motif:HMM:TIGR04183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="T9SS type A sorting domain-containing protein"
                     /protein_id="HPX35052.1"
                     /translation="LENYSHRLQSSLINGVGKDKVYAQFDFCLENYILSTVEKLIFEV
                     FDGNAWHTVHTFTNQGGSIPWSAFNFDISQYAAGKVFMIGFTAEGEDTYNINKILIDN
                     ISLTSELMAVEPSAISDQLVVPTTSQHPITITNLGPKALVWNAAGLPPWASLSASNGT
                     IAGYGNEVIQLNLNSEAASPGVYQVQLTITGAGELASEIIEIQLDILDLVINPTSVND
                     KLQVGETAVWDIEISNPGPYILDWEVNLATKADWAAFDIESGTLQVNEAKVLKLTLDA
                     TQLAQGQYTTDFLFTTADGLIEKTFTVSLTVYDIAFSPEALYDALHVGETSTYPLTIT
                     NTGLEAINWNAVITDNWANLSALSGTIEAGESVTLDFTFNAAGMLPGDYSSSMSFTAF
                     DDKLIKEIIIGLKVLDEDTDFQKIPLPLPNSWGYISTYIDLDKNGKMTLEVALNSILD
                     KMVIMIGHEGFFWPSQNTNTIGNWNLETAYKLKMQKEGFLVFYGSEIEDKNITFPAGT
                     HLVPVINSNPLSAADIFEGRDIEFAFSLDGLIYWPLGNIFTLETLVPGLGYLVKFNSE
                     TTLNFQNAKSSVNQNVVSIFENTTPWNDAYNTANVHLVGVSENAASALQIGDFIGVFN
                     SEGLCTGLTKYVNKDKAFLIPVYGDDFSSEYIDGMKEGEYMTVKVFSNGKVIEVDPVY
                     NTKLSNWDGLYVTNGLSQIADLKMSATGIDADEDVAIRIYPNPSNGIFNISGADASYQ
                     VIVTNAQGQIVYNQKLSSDRLDLTNQPTGVYFIKLIGNNQTVTQKIVIR"
BASE COUNT          939 a          674 c          676 g         1091 t
ORIGIN      
        1 cggtagtatc ttcataggct tctttagtga tctcggaggc aatttcgatc aaacgccgct
       61 gtatgtactt ctggacgact atacgggcat gatactcaat gtttgctgcc gaggcaactc
      121 tacgtgttag ttgggcaata taataagcgc caccgacaat ctcaagctcg ccttttgact
      181 ttagttcgtg agtaacggtg agcatgtcaa ttggcttttg cctggcaaaa agatcttgaa
      241 tagcggagta gatttttttg tgagcatcta aatagaagac ttcaggtttt aaaaaatcaa
      301 tgacggcagt cagagcattt tgctcaagga gtaatcctcc gatcacggct tcttctaaat
      361 caatagcttg tggcggcact ttaccatact ccatcaacgc tgaagttaat gtgcgcgcac
      421 ctaccgaacg cccgtcagat acggttcgca gagacttttc aagaatgctt tttccctctt
      481 cattcattgg ccaaaaatac taaacaactt tcactatttt tgattttttt ttaatttagt
      541 tatcaacaat agtaattttt ttttcaattt tattaagagt gaacaagtta tgaataaaaa
      601 atcaaaatag atgtttttct aataataatg ccttgaaaaa taaatatgga aaagtttgat
      661 tttgccttac attcttgccc ggtaaagtgt aatattcata attttgatct ttcaattcac
      721 tcattcacag aattgctttt tttgaatttc gaaaaccaag ccccttgcat ttcagtgaac
      781 aaagtatttt gatacttcaa ccggttaatt tttttcaccg aacaactttg aatgaaaata
      841 ttgcatacgg ttttcgttac acatattatg tgctcctttt taatattacg gaataagagg
      901 taaaaggttt tgttattgcc gcaaagagat tattagaggc tcatagccat aaaaaaagcc
      961 tgtcggagca attatgacag gcttttttgt aagtatttag atcaggtgtt acctgattac
     1021 aatcttttgt gtaacagttt ggttatttcc aattagctta atgaaataga ctccggtcgg
     1081 ctgatttgta agatcaagcc tgtcggaact cagtttttga ttatagacga tctgcccttg
     1141 agcgtttgtt acaattacct ggtacgaagc gtcagcgccg cttatgttga atattccgtt
     1201 agatgggtta ggatagatac ggattgcaac gtcttcgtca gcgtcaattc cggtagcgct
     1261 cattttcaga tcggcgatct gcgacaggcc gtttgtaacg tataatccgt cccagttact
     1321 caactttgta ttgtacacag gatcaacctc aataactttg ccattgctaa atactttaac
     1381 cgtcatatat tcaccctctt tcattccgtc aatatattcg ctcgaaaagt catcgccata
     1441 tacaggtatc aggaatgctt tatccttgtt tacatatttg gttaaacccg tgcacaaacc
     1501 ttcgctattg aaaacaccta taaagtctcc tatttgcagt gcgctggcag cattttccga
     1561 aacgccgaca agatgcacat ttgcagtgtt ataagcatca ttccatggcg ttgtgttttc
     1621 gaatatcgaa accacatttt gattaacgct actctttgca ttttggaagt tgagtgtggt
     1681 ttcggaattg aacttgacca ggtagcccag tccaggcacg agtgtttcga gtgtgaatat
     1741 attaccgaga ggccagtaaa tcaagccgtc gaggctgaat gcaaattcaa tatcgcggcc
     1801 ttcaaaaata tcggcggcag atagaggatt tgagtttatt acaggtacta aatgcgtgcc
     1861 tgctgggaat gtaatattct tgtcttcgat ttcggagccg taaaacacaa gaaacccctc
     1921 tttttgcatt ttcaacttat aagctgtctc caaattccaa ttgcctatgg tatttgtatt
     1981 ttgcgaaggc cagaagaacc cttcgtgtcc aatcatgatg accatcttgt caagtatgct
     2041 gtttagtgcg acctcaagcg tcatttttcc gttcttatca aggtcaatat atgtagaaat
     2101 atatccccag ctattaggta aaggtaatgg gattttttgg aaatctgtat cttcatctaa
     2161 tactttaagt ccgataatga tctcttttat cagtttatcg tcgaaagcgg taaatgacat
     2221 tgatgaggaa tagtcgcccg gcaacattcc tgcagcatta aaagtgaaat caagtgtaac
     2281 ggactcgccg gcttcaattg ttccgcttaa tgcgcttaga tttgcccaat tatcggttat
     2341 taccgcattc cagtttatag cctccaaacc ggtgttggtt atggttagag gataagtgga
     2401 tgtttcgccg acatgcaatg catcgtacag ggcttccggt gaaaatgcta tatcgtaaac
     2461 agtcaatgaa accgtgaatg ttttttcaat aagcccgtcg gctgtggtaa acaagaagtc
     2521 agtagtatat tgaccctgtg ccaattgtgt tgcgtcaaga gttaatttaa gtactttggc
     2581 ttcattaact tgtagcgttc ctgattctat atcaaaggca gcccaatcgg ctttagttgc
     2641 caggttgacc tcccagtcaa gtatgtaggg gcctgggttg gatatttcaa tatcccacac
     2701 tgcagtttcg ccaacctgca atttatcatt cacagatgtt ggattaatta ccagatccaa
     2761 tatatcaagc tggatttcaa taatttctga tgcaagttcg cctgctcctg ttattgtaag
     2821 ctgtacttga tatactcccg gagatgctgc ttcgctgttg aggttcaact gtataacttc
     2881 atttccgtat ccggcaatag ttccgttaga tgcgcttaga gaggcccacg gaggaagtcc
     2941 ggcagcattc catactaaag cttttggacc aaggttggtt atggtaatgg gatgctgtga
     3001 agtggtagga acaactaatt ggtcagatat tgcggatggc tctactgcca taagctcact
     3061 tgtcagactg atgttgtcaa taagaatctt attgatattg taagtatctt cgccttcggc
     3121 agtgaatcca atcatgaata ctttaccggc tgcatattgg gaaatgtcga agttaaaagc
     3181 gctccaagga atactcccac cctggttggt aaatgtatga acggtatgcc atgcattgcc
     3241 atcgaacact tcaaatataa gcttttcaac tgttgatagg atgtaatttt ctaaacaaaa
     3301 atcgaattgt gcatatactt tgtctttccc aacgccattt attaaactac tctgtaggcg
     3361 atgagaatag ttctcaagca
//
LOCUS       DAOOAI010000139         3360 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_31459_length_3360_cov_5.368835, whole genome shotgun sequence.
ACCESSION   DAOOAI010000139 DAOOAI010000000
VERSION     DAOOAI010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31459_length_3360_cov_5.368835"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..228)
                     /locus_tag="PK345_08295"
     CDS             complement(<1..228)
                     /locus_tag="PK345_08295"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HPX35053.1"
                     /translation="MRNFIYHNPTKIIFGENVLHKISAEIPANARIMLTYGGGSIKKN
                     GIYHQVVSVLEDFLFIEFGGIEPNPQYSTLMK"
     gene            complement(242..994)
                     /locus_tag="PK345_08300"
     CDS             complement(242..994)
                     /locus_tag="PK345_08300"
                     /inference="COORDINATES: protein motif:HMM:NF013509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase
                     N-terminal domain-containing protein"
                     /protein_id="HPX35054.1"
                     /translation="MSFKNFYYQPVFSSKLKGNIFSVILIMFILSACHQEVENVEQIE
                     NIIPTTRMDTISYIMGYDYGVGIAEKEIGANPLLIYKGLYDALNDRQPLLDDTLQNRL
                     IEEFSFELEEIEKQRFEVMLAQNRKKGEEFLLINKEKEGVHSLPSGLQYKILKFGNGP
                     YPMLDDSISIHYRAMYIDRTTFDMTYDEGPVNVKLSHLVKGLTEGIRLMQTGAIFEFY
                     LSPDIAYGNSNYLDLIPAGSTVIYTVELIKIF"
     gene            1440..>3360
                     /locus_tag="PK345_08305"
     CDS             1440..>3360
                     /locus_tag="PK345_08305"
                     /inference="COORDINATES: protein motif:HMM:NF025006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase regulatory-like
                     domain-containing protein"
                     /protein_id="HPX35055.1"
                     /translation="MKQFYCILTIALCLLVVNTFAQQVLLQQTVTDASKGQIVLTEKA
                     STQSPINESVILGGTSKSPNINWRLTESIAIGANLRISQEAQKTFISWYLNNSRASLY
                     GNSPTPIFEIPIDNDFVEAPIDMTADGLYLALGTDNKLRVFDGETHTLIWEKDFEGYV
                     LAFKIAEGGQKIYLAETEVAGEAKSRASAYIIGQDDPLWTTDFEGNATAFAISGDYSK
                     IAFCQYSGVNKMHILNGENGEVIFDAFYFNQNPPAFNYDGKFVILGDYSGKAYLYEYD
                     EDGDTYFEKWSFKVGGGGSSAWVVAASISADGSTVAIGTLVFLSADYNGEIYIFNTYS
                     NEPIWVYEDCGDSIGSISLSDDGSLIAATTWGPMDHSKPDYFLFRKQSNVPIFTINTV
                     GSLYSVVLSPDGTYCSVTGKRVHARMMGCGGFLYNINTDPGGGKLTGNIQLQGADSFE
                     NAKIVIEGIDNYYGYSDEEGNFEIKYIPAGSYTVTASKVGFYPDQADVVIDEGGITTI
                     DFSLLPTGNPPVNLSATQGADYTISLNWEHPDPSATTGFNIYRKSIAEELFPETPIAT
                     TDNNLLEFIDADVQPLITYYYVVTAILDQDSESPYSNEVQGWMASGFMTNEISAYVGT
                     TPVIDGTLSPGEWDDA"
BASE COUNT         1012 a          618 c          640 g         1090 t
ORIGIN      
        1 cttcataaga gtagaatatt gcggatttgg ttcaatacca ccaaactcaa taaataagaa
       61 gtcctcgaga acgctgacta cctgatgata aatcccgttc tttttgattg atccaccgcc
      121 gtaagtaagc ataatacgtg cattggcagg gatctcagcg gaaattttat gtaaaacatt
      181 ttcgccgaag ataatttttg ttggattgtg gtaaataaaa tttctcataa ttaactttgt
      241 tctaaaaaat tttaatcagc tctacggtat aaatcactgt tgatccggca ggtatcaaat
      301 caagataatt actattgcca taggcaatgt ctggtgaaag ataaaactcg aatatagcgc
      361 ctgtttgcat tagcctaata ccctctgtaa ggccttttac caaatgactg agtttaacgt
      421 ttacaggccc ttcatcatag gtcatatcaa aggtagtacg atcaatatac attgcgcggt
      481 aatgtatgct gatactatcg tcgagcatgg gataggggcc gtttccaaac ttcagtattt
      541 tgtattgcaa cccgcttggc agtgaatgaa caccttcttt ttctttgttt ataagaagaa
      601 attcttcacc ctttttcctg ttttgtgcaa gcattacttc aaacctttgc ttttcgatct
      661 cttctaattc aaaagaaaac tcctcaataa gacgattttg caaagtgtcg tccaacaaag
      721 gctgcctgtc gtttaaagca tcgtaaagtc ctttataaat aagtaatggg ttggcgccga
      781 tctccttttc agcaattcca acaccatagt catatcccat aatataactt attgtgtcca
      841 tacgggttgt aggtattata ttttcaatct gttctacatt ttcaacttcc tgatgacagg
      901 cgcttagaat aaacattatt aagatgaccg aaaagatatt tcccttgagt ttggaggaga
      961 aaactggttg atagtaaaaa tttttgaaac tcatgatcaa cttgttttta tgaataacac
     1021 attgaattca cttaaatctt tctttaaata ataatctctt atttctttta aaaatgtaat
     1081 cgcgtaattt ttttgtcgga tcatgctaaa aaaaaatcaa aatgtacatt gcaccaagat
     1141 atcataaccg attgttctaa attattgtta aaaatttaat catgattttg acattggcaa
     1201 atgcgcaatc tggattaatt ccctacgcaa atatatttta tttaaatcaa cagtgtgtta
     1261 cttttgaaat tgctcatatt cctctaaaat tgtaatcttt tcacattgtt gaaaatcaaa
     1321 cataagttcc atttttatct tcaatggatt tttatatgaa gcagaggtta ttttaattta
     1381 taatagagaa gtttattaat tttgctacca ttaattttat taatcaacat tttacaccta
     1441 tgaaacaatt ttattgcatt ttgacaattg cgttgtgtct gttggtggtt aacacttttg
     1501 cgcagcaagt tttacttcaa caaactgtaa cagatgcaag taaaggacaa attgttctta
     1561 cagaaaaggc ttctacacaa tccccaatca atgaatctgt tattttaggc ggcacttcca
     1621 agtctcctaa tattaactgg cgactcactg agtcgattgc tattggggcg aaccttagaa
     1681 tttctcaaga ggctcaaaaa acttttatct catggtattt aaacaattcc agagcctcac
     1741 tgtatggtaa tagccccacg ccaatctttg aaattccgat tgacaatgat tttgtggagg
     1801 ctccgattga catgaccgca gatggattgt acttagcctt aggaactgat aataaattga
     1861 gggtttttga tggggaaact cataccctca tttgggaaaa agattttgaa ggttatgtac
     1921 ttgcgtttaa aattgctgag ggggggcaaa aaatttattt ggcggagact gaagttgctg
     1981 gagaagcaaa atcaagagca agcgcttata tcatcggcca ggatgatcct ctttggacaa
     2041 ccgacttcga gggtaacgca accgcttttg ctatatcagg cgattattct aaaattgcat
     2101 tttgccaata ttcaggggtt aacaaaatgc acatcttaaa tggtgagaat ggtgaagtga
     2161 ttttcgatgc attttatttc aaccaaaatc caccggcttt caattatgat ggcaagtttg
     2221 ttattttggg tgattatagt gggaaagctt atctgtacga gtatgatgaa gatggcgaca
     2281 cctattttga aaaatggagc tttaaagttg gcggtggtgg atcatcagcc tgggttgttg
     2341 ctgctagcat ttcagccgat ggttcaacgg tagcaatagg tactttggta ttcctctctg
     2401 ccgattacaa tggagagatt tatattttta acacctattc caatgagcca atttgggttt
     2461 atgaagattg tggtgattca attggttcaa tttctttatc tgatgacggg tcgctgattg
     2521 cagcaaccac ttgggggcca atggatcatt caaaaccaga ttattttcta tttcgaaaac
     2581 aaagtaatgt tcccatattt acaattaata cggtaggctc actctattcg gttgttcttt
     2641 cacctgacgg cacctattgc agcgttacag gcaagagagt tcacgctcgg atgatggggt
     2701 gtggcggttt tttatataac attaatactg atccgggagg aggtaaactt accggtaaca
     2761 tacaacttca aggcgctgat agctttgaaa atgctaagat agttattgaa ggtattgaca
     2821 actattacgg atattctgat gaggaaggca actttgagat aaaatatatc cctgccggtt
     2881 catatactgt tacagcatca aaagttggct tttatcctga tcaagccgat gttgtgattg
     2941 acgaaggcgg cattaccacc attgattttt cacttctccc gacaggaaat ccacctgtaa
     3001 atttatctgc tacacaaggg gcggattata ccatttcgct caattgggaa cacccagatc
     3061 cctccgcaac aacaggtttt aatatctatc gtaagtctat tgcagaagag ctttttccgg
     3121 aaacgcccat tgcaacgaca gataacaacc ttcttgagtt tatagatgct gacgtgcaac
     3181 cattgattac atattattac gttgtaacag ctatcctcga tcaagactct gaaagcccat
     3241 actctaacga ggttcagggt tggatggcat caggctttat gacaaacgaa atcagcgcat
     3301 acgtaggcac aactcctgta atagatggaa ccttgtcgcc aggcgaatgg gacgatgctt
//
LOCUS       DAOOAI010000140         3352 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_31545_length_3352_cov_4.487413, whole genome shotgun sequence.
ACCESSION   DAOOAI010000140 DAOOAI010000000
VERSION     DAOOAI010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3352)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3352
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31545_length_3352_cov_4.487413"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..543)
                     /locus_tag="PK345_08310"
     CDS             complement(<1..543)
                     /locus_tag="PK345_08310"
                     /inference="COORDINATES: protein motif:HMM:NF024872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase domain-containing protein"
                     /protein_id="HPX35056.1"
                     /translation="MAKTKTLFYCQNCGYQSSKWMGKCPSCENWNTFVEEVIISGSKP
                     ISPVLSGFTRSKPKQLNDIETGNEFRINTADHELNRVLGGGLVLGSIVLVGGEPGIGK
                     STLMLQLALNLRNQRALYVTGEESEQQIKMRSLRLGTENPTCFILTETSLEDIFLQIE
                     NIKPDIVIIDSIQTLRTQVID"
     gene            complement(557..1051)
                     /gene="rfaE2"
                     /locus_tag="PK345_08315"
     CDS             complement(557..1051)
                     /gene="rfaE2"
                     /locus_tag="PK345_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018337873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase"
                     /protein_id="HPX35057.1"
                     /translation="MKKLEIVETKIHFNHDTLKTWLAYWRFKEKKIVFTNGCFDLLHQ
                     GHVDYLSKAADQGNILIVGLNTDASVSRIKGSGRPVQDEKSRSLILAALRFVDAVVLF
                     DEDTPLELIEFVKPDVLVKGSDYAAEDIVGYDIVKANGGEVITIDFLEGYSTSALIER
                     IKKL"
     gene            complement(1038..2048)
                     /locus_tag="PK345_08320"
     CDS             complement(1038..2048)
                     /locus_tag="PK345_08320"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HPX35058.1"
                     /translation="MKRRIFSALKILFFLSIGVFFIWIFVRKLTPDQKQEIWASFISA
                     NYFWIIVSIILGILSHLIRSLRWNMLLEPMGYKPKLKNTFSAVMIGYLTNTAIPRLGE
                     VTRCGILSKYEKIPLSKSFGTVIVERSLDLVVFAVLFFFNLIMFYDKLGRYVEDRILI
                     PMRNKFDIENNILAIVLISCIVLFILFFIFRKRIQHLKIYHKSKEIIHGFWHGLWVVT
                     KIERPFTFILYTLLIWFLYYMMIYVCFFTMPQTNHLGFDVALSLLMFGSIGIIVVQGG
                     IGIYPAIIAETLILYDVPTTYGYTLGWLGWTAQTLMIVITGLVCLVLLPLINKSKEYE
                     KA"
     gene            complement(2052..2399)
                     /locus_tag="PK345_08325"
     CDS             complement(2052..2399)
                     /locus_tag="PK345_08325"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005861624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004068 - aspartate 1-decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006523 - alanine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HPX35059.1"
                     /translation="MQIEILKSKIHRATLTGANLKYIGSISIDEELMEAANLIENEKV
                     HVYNITNGERLETYVIKGVRGSGQISLNGAAARKAETGDLVIIVSYASMDFEKAKSFK
                     PHIIFPDHNNKLS"
     gene            complement(2415..3257)
                     /gene="panC"
                     /locus_tag="PK345_08330"
     CDS             complement(2415..3257)
                     /gene="panC"
                     /locus_tag="PK345_08330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013612913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HPX35060.1"
                     /translation="MKILNSVADVKTYISTIKALGRSIGFVPTMGALHEGHLSLIIRA
                     HEDNDITVASIFVNPIQFNNKTDLAKYPRPIEKDIQKLEEIGCDMLFYPTVEEMYPEP
                     VFDKYDFGQLDKVMEGKFRPGHFNGVAVVVRRLFDIVTPDKAYFGMKDYQQLRIIQTM
                     VSTLKLPIEIIPCPIIREKDGLALSSRNVRLTKKQRSIAPAIFKVLSEIKDNARKTTV
                     EEAENWAISKLNKYDEMFVEYLSIVDSETLLPIKDWDSTRSCLACVAVFLGEVRLIDN
                     VMIF"
BASE COUNT         1068 a          703 c          557 g         1024 t
ORIGIN      
        1 atcaataact tgagtgcgca gcgtttgaat tgaatcaata attacaatat caggttttat
       61 attttcgatt tgcaaaaaaa tatcttcaag ggaagtttca gtaagtataa agcatgttgg
      121 attttctgtt cctaagcgta atgagcgcat cttaatctgc tgttcactct cttcgcctgt
      181 aacataaagc gctctttgat tgcgcaggtt aagcgctaac tgtagcataa gtgtagattt
      241 tccaatgcca ggctcccctc ccaccaacac aatggaacct aaaaccaaac caccaccgag
      301 tactctgttt agctcatggt cggcagtgtt aatccgaaat tcatttccgg tctctatatc
      361 atttaattgc ttaggcttac ttcgggtaaa acctgataac acaggactga taggctttga
      421 tccggaaatg ataacctctt cgacaaaagt gttccaattt tcgcacgaag ggcatttacc
      481 catccacttg gacgattgat agccacagtt ttgacaataa aaaagtgttt tggttttagc
      541 catagtaaaa aaatatctac agttttttga tcctttcaat caaagcgctt gtagaatagc
      601 cctccagaaa gtcaatagtt attacctcac cgccattcgc ttttacaatg tcgtagccaa
      661 caatatcttc ggctgcataa tcacttccct taactaacac atcaggctta acaaactcta
      721 taagctctaa tggagtatct tcatcaaaga gtaccactgc atcaacaaag cgtaacgccg
      781 caagtatgag tgagcgcgac ttttcgtcct gaacaggcct gcccgaacct tttatacggc
      841 tgacagaagc atcggtattt agtcctacta tcaagatatt cccttgatcg gcggctttgg
      901 aaagataatc aacatgccct tgatgcaaca agtcgaaaca tccatttgta aaaacaattt
      961 ttttctcttt gaacctccag tatgcaagcc aggtcttcaa tgtatcatga ttgaaatgta
     1021 tctttgtttc aacaatttca agctttttca tattctttcg atttattgat taaaggtaat
     1081 aatactaaac atactaaacc ggttataacg atcatcagtg tttgcgctgt ccatcctaac
     1141 cacccgagtg tataaccata agttgttggg acatcgtaca aaataagggt ttcagcaatt
     1201 atggccggat aaatgccaat accaccctgt acaacaataa ttcctattga cccaaacatc
     1261 aatagcgaca gagcgacatc aaaacccaag tggttagttt gtggcatggt aaaaaaacaa
     1321 acataaatca tcatataata aagaaaccag ataagcaaag tgtaaaggat aaaggtaaat
     1381 ggtctttcaa tttttgtaac aacccataac ccatgccaaa acccgtgtat tatctctttc
     1441 gatttatgat aaattttaag atgttgaatt ctttttctaa aaatgaaaaa taaaataaat
     1501 aaaacaatgc aagatatcag aacaatagcg agtatattat tttcgatatc aaacttattc
     1561 ctcataggta tcagtattct atcttcgaca tacctaccta atttatcata aaacattatc
     1621 aaattaaaaa agaagagtac tgcaaacact acaaggtcaa gactccgttc aactataaca
     1681 gttccaaatg atttgctcaa gggaattttt tcatatttgc tcagtattcc gcaacgtgta
     1741 acctctccaa ggcggggaat agcagtgttg gtaaggtagc cgatcataac agcggaaaag
     1801 gtatttttta gcttcggctt ataacccatc ggctcgagaa gcatattcca acggagtgaa
     1861 cggattaaat gacttaaaat acctaaaata atggatacta tgatccaaaa ataattggcg
     1921 ctaataaaag atgcccaaat ctcctgtttc tgatctggtg taagttttct tacaaatatc
     1981 cagataaaaa agaccccgat tgagaggaaa aacaggatct ttaatgctga aaagattctt
     2041 cttttcaatt tttaactcag tttgttgtta tgatcaggga atataatatg cggtttgaat
     2101 gatttagctt tttcaaaatc cattgatgcg tacgaaacga ttataacgag atcgccggtt
     2161 tcagcttttc gtgctgcagc cccatttagc gatatttgcc ccgagccccg cactccttta
     2221 attacatatg tctcaaggcg ctcgccattg gttatgttat aaacatgtac cttctcattt
     2281 tcgataaggt tggcagcctc catcagctct tcatcaatgg agatactacc gatatatttc
     2341 aagttggcgc cggtgagcgt tgccctgtga attttcgatt taagtatttc aatttgcata
     2401 aggcagcaaa attactaaaa aatcataacg ttgtctatca acctgacctc tccaagaaaa
     2461 acagcaacac aagccaaaca actccttgta gaatcccaat ctttaatagg gagtaatgtc
     2521 tctgaatcca caatagacag atattcaaca aacatctcat catacttgtt cagttttgaa
     2581 attgcccaat tctcagcctc ttctacagta gtctttctag cattatcttt tatctcggat
     2641 aacactttaa aaatggctgg agctatgctg cgttgttttt ttgttaatct gacatttctc
     2701 gagctaagag ctaatccatc tttttcgcga ataattggac acggaataat ctctattggc
     2761 aatttcaaag tagagaccat ggtttgtata atacgtagtt gctgatagtc cttcatacca
     2821 aaataagcct tgtcgggtgt aacaatatca aataacctcc ttacaacaac agctacacca
     2881 ttaaaatgac caggcctgaa tttaccctcc atcactttgt cgagctggcc aaaatcatac
     2941 ttgtcgaata caggctcagg atacatctct tcaactgtag gataaaacag catatcgcat
     3001 ccaatctctt caagtttctg gatatccttt tcaataggtc tcggatattt tgccaagtcg
     3061 gttttgttat tgaactgtat tggatttaca aaaatactcg caacggtgat gtcattatct
     3121 tcatgcgccc gaattataag agatagatgc ccttcatgta aagctcccat tgttggtacg
     3181 aatccaatag agcgccccaa tgcctttata gtcgaaatgt aagttttaac gtcggcaaca
     3241 gagtttaaaa tcttcattga tcaacaatta ctgtgcttgc attaaaacaa aaagcatgtc
     3301 tttctcaagt gaaaaggtag gtgtttctat tactcttccc acctcaacat ct
//
LOCUS       DAOOAI010000141         3350 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_31574_length_3350_cov_5.075569, whole genome shotgun sequence.
ACCESSION   DAOOAI010000141 DAOOAI010000000
VERSION     DAOOAI010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3350)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3350
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_31574_length_3350_cov_5.075569"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1139)
                     /locus_tag="PK345_08335"
     CDS             complement(<1..1139)
                     /locus_tag="PK345_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX35061.1"
                     /translation="MKKIGLITLLVYLFANLAYAQNEEDALRYSNVHLLGTARYMGLS
                     GAYGAIGADFSTLSSNPAGIGLYRRSEFSITPLLHIAKTTSTYNNERHDDNKSNFGLG
                     NLGFVLTFETKKKLDQNPIRNFQFGVGINRIKDFSNRINIKGINKVNSLLDTYLIYAG
                     NMNPENLDVFDTRLAFDTYLLDTIIGASVPTYVNAYSYIGGFNGAIQRKTIETSGSIY
                     EWALSGGMNFNDVLYFGLTLGFPRLKYYQESVYSEFNLTEPNKDLDQFNYAEELKTTG
                     TGFNVKTGVIVRPADWLRVGLAYHSPTWYNDLHDNWKTSMKAMYTNGDIFTSKSPTGE
                     FFYDIETPWKIIASAAIIVSNKGFISADYEFTDYRNSSVKPNFTY"
     gene            complement(1184..2779)
                     /locus_tag="PK345_08340"
     CDS             complement(1184..2779)
                     /locus_tag="PK345_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HPX35062.1"
                     /translation="MKRLIYLLGAMIIMISCTTSKDAMTVYDDVYYSPKNQVVTTQKT
                     IPDYQVTYSPERASEEYQQSSSPQSQPVETNATSSYKSSEYLDNYYPESTDQEYYYDD
                     DQSNYYSDYDYEARLKRFYRANIGFGYYDPYYTGFYSPYYGPGFSFSYGWGWPSTYFS
                     FGYSWGPRYPYYYYNPWCWDPWYSWGYYPYYRPYWYSPYWYGSYGYGYWNGYWDGYIG
                     GSTGYYPEHQNHYYGPRNPRGSSIIGNPEPRASRISSDDEIKNTYISGRASRTEGSST
                     ITGDPNGTSNNRIIRPQDAQVSGTEIISNKGKEERTNIFSTSEPKPTTPEVSRNAENI
                     KPQEPKEGTTTRSVVPISGRQQGNAVNNEKYSKPQQANKDNVFFRERIYAKPQSNDLE
                     RSMTIPKTYSAPNANRPRSSNEYVVPNSRNKQNINRSSESRQGTVTTPSNQRNFSTPS
                     RSQNKFSQPNRSTSSPFSQPSRISTPSEPTHRMSTPSRSSQNYSAPSQSRSSGSYSAP
                     SSSGRSSQGSSSSGSSSSSRGRR"
     gene            2913..3299
                     /locus_tag="PK345_08345"
                     /pseudo
     CDS             2913..3299
                     /locus_tag="PK345_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010537985.1"
                     /note="catalyzes the formation of prolyl-tRNA(Pro) from
                     proline and tRNA(Pro); incomplete; partial in the middle
                     of a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
BASE COUNT          912 a          601 c          754 g         1083 t
ORIGIN      
        1 ctatatgtga agttgggctt gacagaagag tttcgataat cggtaaactc gtaatcagcg
       61 ctaataaatc ctttgttact tactatgatg gcagcgcttg caattatttt ccatggcgtt
      121 tcaatgtcgt agaaaaattc tcctgttggt gatttcgaag taaaaatgtc gccattagtg
      181 tacatggctt tcatggatgt tttccaatta tcatgaagat cgttgtacca agtaggcgag
      241 tgataggcaa gtccaacacg cagccaatct gccggacgga caataacacc ggttttgacg
      301 ttaaatccgg ttccggtggt tttaagctct tcggcataat taaactgatc aaggtcttta
      361 ttgggttcgg taaggttaaa ttcactatat accgactcct gataatattt tagtcgcggg
      421 aatcctaatg tgaggccaaa atatagtaca tcgttaaagt tcatcccccc cgaaagcgcc
      481 cactcgtaga tagagccgct tgtttcgatc gttttacgtt gtattgcgcc gttgaagccg
      541 ccgatatagc tgtatgcatt aacgtaagta ggaacagatg cgccaattat tgtgtcgagc
      601 aggtaagtgt cgaaagctaa gcgggtatca aatacatcca aattctcggg attcatgttg
      661 ccggcataaa taagataagt atctaatagt gagttaactt tattaatgcc tttgatattg
      721 atacgattgc tgaagtcttt aattctgttt ataccgacgc caaactggaa gttcctgatc
      781 ggattttgat ctaacttttt tttagtttcg aaagtcaata caaaaccgag attacctaag
      841 ccgaagttcg atttgttatc atcgtgtcgt tcgttattat atgtgctggt agtttttgca
      901 atgtgcaaaa ggggtgttat agagaactct gatcgtctgt acaggcctat acctgcagga
      961 ttggagctaa gtgttgaaaa atctgcgcca atagctccgt atgctccgct taaacccata
     1021 taacgtgcag ttcctaataa atggacattg gagtatctga gtgcgtcttc ctcgttttga
     1081 gcataggcaa gattagcgaa tagatatacc aaaagcgtta ttaaacctat ttttttcatt
     1141 tgtctgagtg atttaattaa taagataaat aatgtcagtt cttctatctc ctacctctgc
     1201 tacttgatga gctacctgaa gaggaagacc cttgtgatga tctgcctgaa ctggaaggag
     1261 cgctataact accggatgat cgtgattgtg agggcgccga atagttttgt gatgatctgg
     1321 atggtgttga cattctatgt gtcggttccg aaggtgtgct tatccttgat ggttgtgaga
     1381 atggcgaact tgtagatcgg ttcggctgcg aaaatttatt ttgagaacgc gatggtgtgg
     1441 agaaattcct ttgattggaa ggcgttgtta cagtaccttg acggctttct gatgatctgt
     1501 ttatattttg tttattcctc gaatttggta caacgtattc gttactcgag cgaggacgat
     1561 tggcattagg cgctgaataa gttttaggta ttgtcattga tctttcaaga tcattagatt
     1621 gtggcttggc ataaattctc tcacggaaaa aaacgttgtc cttattagcc tgttgtggtt
     1681 tagaatattt ctcattatta actgcattcc cttgctgtct tcctgaaatt ggaactaccg
     1741 aacgtgtagt tgttccctct tttggctctt gtggtttgat gttctcggca ttgcgtgaaa
     1801 cctccggtgt tgtaggctta ggctcggatg tcgaaaagat attcgtcctc tcttcttttc
     1861 ctttgtttga aataatttct gtgccgctaa cctgagcgtc ttgtggtctg attattctgt
     1921 tatttgaggt gccattaggg tcgccggtaa tcgtagagct tccctcagta cgtgatgcac
     1981 gcccggagat ataagtgttt tttatttcat cgtcagatga aatccttgat gcacgtggtt
     2041 cgggatttcc aatgatactg ctgcctcttg gatttcttgg gccataataa tgattttgat
     2101 gttcgggata ataacctgtt gaaccgccga tgtaaccatc ccaatatcca ttccagtaac
     2161 catatccata ggagccgtac caatagggcg aataccaata aggacggtaa taggggtaat
     2221 atccccacga gtaccatgga tcccaacacc agggattata ataatagtaa ggatatcgtg
     2281 gcccccagct atagccgaag gagaaataag ttgatggcca gccccagcca taagaaaacg
     2341 agaatcctgg tccgtaataa ggggaataaa atccagtgta ataaggatcg taataaccga
     2401 atccgatatt agctctgtaa aaccgtttta gccttgcttc atagtcataa tcagaatagt
     2461 aattactttg atcgtcatcg taataatatt cctggtctgt tgattcggga tagtagttat
     2521 ccagatattc agacgattta tagctggatg ttgcatttgt ttcaacaggt tggctttgtg
     2581 gcgatgaaga ttgttggtat tcttcagacg ctctttcagg gctgtatgta acctgataat
     2641 cggggattgt cttttgtgtt gtaacaactt gatttttggg agagtaatag acatcgtcat
     2701 aaacggtcat ggcatctttg ctggttgtgc aggagatcat tatgatcatt gcgccgagta
     2761 gataaattaa acgtttcatt gcagttcctc ctttaaatat ttttgtttcg ttatgtaaaa
     2821 ctatgttcaa aatcaactgg gttaataatt ttctattttt gcatcataaa aatttcgata
     2881 aaaaagttcc aaaaattata ccaaaaagta ttatggcaaa agatttttca acacgtgctg
     2941 agaattacgc acaatggtac aacgatttgg tgataaaagc tgaccttgct gagaactccg
     3001 cagtgagggg ttgtatggtc ataaagcctt atggttatgc gatttgggaa aagatgcagg
     3061 cagcgcttga taagatgttt aaagatacag gacattcaaa tgcttatttt cctcttttta
     3121 ttcctaaatc atttttaagc aaagaggctg atcacgtcga aggctttgcc aaagagtgtg
     3181 ccgtagttac acattatcgg ttaaaaaatg ccgatgacgg aaaggggatt gttgtagatg
     3241 aggatgccaa gcttgaagag gagttgatca ttcgtccgac ttctgagact attatctggt
     3301 tgataaggag aggcaaatca cgataggact gtatccaatt acgatatgta
//
LOCUS       DAOOAI010000142         2997 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_36472_length_2997_cov_5.590415, whole genome shotgun sequence.
ACCESSION   DAOOAI010000142 DAOOAI010000000
VERSION     DAOOAI010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2997
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36472_length_2997_cov_5.590415"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /locus_tag="PK345_08350"
     CDS             <1..754
                     /locus_tag="PK345_08350"
                     /inference="COORDINATES: protein motif:HMM:NF013073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HPX35063.1"
                     /translation="PVRAKESHSSEMVNQLLFGETFEILETLNEFVLIRGTLDNYEGF
                     ISRKQFLPLTKDEYEMLMSKDKLFPVNPISTITETNSGNTIKILHGSSLRGFEDGRLV
                     IASQSFFTTDPMKSPDTVLDTEMLVQSAFQFLNAPYLWGGRSLFGIDCSGLTQIVYNI
                     HGIGLQRDSRCQAQQGETIHLLSEALSGDLAFFDDDEGNITHVGIIINNRKIVHASGM
                     VRIDDLDHHGIFNQELKKYTHKLRLIKRMLGL"
     gene            complement(941..1438)
                     /locus_tag="PK345_08355"
     CDS             complement(941..1438)
                     /locus_tag="PK345_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007659302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HPX35064.1"
                     /translation="MKEKSIELLNKAVGEELTAVHQYMYFHFHCDDQGYDLLANLFKR
                     TAIEEMKHVEDLAERILFLKGEVKMEVSEPNKYITNVKEMLTLARKMEEDSTLHYNKW
                     ANECAQNADSVSKKLFEKLVEEEERHYDQYDIEIENMEKFGDKYLVLQSIERSRATSS
                     GTTAD"
     gene            complement(1717..2202)
                     /locus_tag="PK345_08360"
     CDS             complement(1717..2202)
                     /locus_tag="PK345_08360"
                     /inference="COORDINATES: protein motif:HMM:NF025491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4293 domain-containing protein"
                     /protein_id="HPX35065.1"
                     /translation="MLQRIQTLFLLIVIIAGIVLMFVPLAVYYSEMGYYIKFFLYGVK
                     NLAHDPFGAESPRLFSAFFGKPLLIVQIVNIIIVSFTIFKFKKRTLQLKLNQLNIFLH
                     VILVGGIFYYSTIIETKVQATPVYGLGVTLPLISIIALFLANYFIRKDERLVRSADRL
                     R"
     gene            complement(2301..2750)
                     /gene="aroQ"
                     /locus_tag="PK345_08365"
     CDS             complement(2301..2750)
                     /gene="aroQ"
                     /locus_tag="PK345_08365"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016277328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HPX35066.1"
                     /translation="MKIRIINGPNLNLLGRREIEIYGIRSFDEYLDSLKKIFTNYTLD
                     YFQTNVEGEIINSLHEAEFNYDGIIINAGGYTHTSIAIGDAIKALTIPVVEVHISNIF
                     SREQFRHTSFIAPNAAGIIAGFGLDAYRLAILALENIGKKSKGLQAL"
     gene            2809..>2997
                     /locus_tag="PK345_08370"
     CDS             2809..>2997
                     /locus_tag="PK345_08370"
                     /inference="COORDINATES: protein motif:HMM:NF016168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF434 domain-containing protein"
                     /protein_id="HPX35067.1"
                     /translation="MISQNFLHAVRDYQYLVDQGYLPRNILDLVGNRHELKAVERSML
                     YRGIFSEISCQKRSGKMVK"
BASE COUNT          952 a          541 c          561 g          943 t
ORIGIN      
        1 ccctgttcgt gctaaagaaa gccatagttc ggaaatggtg aatcaattgc tgtttggaga
       61 aaccttcgag attctcgaaa cgttgaacga gttcgtgtta ataagaggaa ctttagataa
      121 ctatgaaggt tttataagcc gtaaacagtt tctgccactc acaaaagatg aatatgaaat
      181 gttgatgagt aaggataagt tgtttccggt taatccgatc tctacaatta ctgaaacaaa
      241 tagcggtaac actattaaaa ttttacacgg tagcagcctc aggggctttg aagatggaag
      301 acttgttata gcgagccaat cctttttcac taccgatccg atgaaatcgc ccgataccgt
      361 tttagatact gaaatgttag ttcagtcagc ttttcagttc ttaaatgcgc catatctttg
      421 gggaggacga tctctctttg gaattgattg ttcggggctg acgcagattg tttacaacat
      481 tcatggtata ggcttgcaga gagattcacg ctgccaggcc caacaaggcg aaactataca
      541 tctgctctcc gaagcgcttt ccggcgattt ggcattcttc gatgatgatg aaggaaacat
      601 cacccatgta gggataatta tcaataatcg gaagatcgta catgcatcag gtatggttag
      661 aattgatgat ttagatcatc atggcatttt taatcaggaa ttgaaaaaat atacacataa
      721 gttaaggctg ataaaacgta tgcttgggct ttagggcaaa atggtaatta aaaaataaca
      781 caaaataaaa accccgtaaa gctattgttt tacggggttt tctgacttta agcttagctt
      841 tgctttttgc cccatattaa gcgtttgata acactttata gggattagca ttatgaaaaa
      901 agttgcctct aaaaaaactt tctatttatt aaattaaggt ttaatctgct gtagtgcccg
      961 atgaggtcgc gcgactgcgt tcgatagact gcaagacaag atatttatct ccaaactttt
     1021 ccatattctc tatctcaata tcatattgat cgtagtgacg ctcctcttcc tcaactaact
     1081 tctcaaagag tttcttcgat actgagtcgg cgttctgagc acattcattt gcccatttgt
     1141 tgtaatgcaa agtgctgtcc tcttccattt tacgcgccaa ggtcagcatc tccttaacat
     1201 tggttatgta cttgttaggt tcactgactt ccatttttac ctcgcccttt aagaaaagaa
     1261 tacgctcggc aaggtcttca acatgtttca tctcttcaat agctgtgcgc ttgaaaagat
     1321 tggctaagag atcatacccc tgatcgtcgc agtgaaaatg aaaatacatg tactgatgaa
     1381 cagccgtaag ttcttcacca acagccttgt ttaataattc tatacttttt tctttcatga
     1441 ttttgatgta ttttgaataa ataaaaaatg tttcaaaaaa tcagcctgta tcatattttt
     1501 gcaggttgtt ggcaaagata tgatttttca agtttaaaaa agaactttaa gagagttttg
     1561 aaactataaa aagttgagcc ccctttttga ctatcctata attttaggat atagtttacc
     1621 ggattgaatc actcgaaatg ataattcatt caatcctatg atattttatc aaaaagatcg
     1681 gtttggcaag ggcaaaaccg gagcgtgctg aattgtctat ctcaatcggt cggcagagcg
     1741 aacaagtcgt tcatctttac ggataaaata gtttgccaga aatagtgcaa taatggaaat
     1801 aagcggaaga gttacaccta atccataaac cggagtagct tgcacctttg tctcaattat
     1861 agtggaataa taaaaaattc ctccaaccag tatgacgtga agaaatatgt ttaactggtt
     1921 cagtttgagt tgcaatgtac gcttcttgaa cttaaaaatg gtaaaactta cgataataat
     1981 gttaacaatc tgtacgataa gcaaaggctt tccgaaaaag gcgctgaaaa gtcttgggga
     2041 ttcggctcca aaaggatcgt gtgccagatt ttttacaccg taaagaaaaa acttgatata
     2101 atatcccatt tccgaataat agactgccag cggaacaaac atcaaaacaa ttccggcaat
     2161 tatcacaatc agcaaaaaga gcgtttgtat tctctgaagc ataataagag ttattttaat
     2221 taggttgcaa atatagtatt taagcgttta aggcttttct tatcattttc catacgataa
     2281 aaaacaaaag attgtaacag ttataatgcc tgaagccctt tgcttttttt accgatattt
     2341 tcaagagcta aaatagctaa tcggtaggcg tctaatccga aaccggcaat tatacctgct
     2401 gcatttggtg caataaatga ggtgtgcctg aattgttcac gactgaaaat attgcttata
     2461 tgcacttcaa caacaggtat ggttaaggct ttgattgcat caccaattgc aatagaggta
     2521 tgcgtatatc cacctgcatt tatgataata ccatcgtaat taaattcagc ctcatgtaga
     2581 gaattgataa tttcgccctc cacattcgtt tggaaataat caagtgtata atttgtaaat
     2641 atttttttca gactatcaag atattcgtca aatgatcgaa tgccataaat ttcgatttcg
     2701 cgcctgccaa gcaaattcag attaggaccg ttaatgattc gaattttcat agttttgttt
     2761 tttatccgac aaaagtaacc aaatcagccg tacataattt tgacaataat gatttcccaa
     2821 aactttcttc atgcggtaag agattaccaa tatcttgttg atcagggcta tctgccaaga
     2881 aacatactcg atcttgttgg aaatcgtcat gagttaaagg cagtagaacg atcaatgctt
     2941 tatcgaggga ttttttctga aataagttgt caaaagcgct ccggtaagat ggtaaag
//
LOCUS       DAOOAI010000143         2212 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_54227_length_2212_cov_4.859527, whole genome shotgun sequence.
ACCESSION   DAOOAI010000143 DAOOAI010000000
VERSION     DAOOAI010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54227_length_2212_cov_4.859527"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(356..706)
                     /gene="folB"
                     /locus_tag="PK345_08375"
     CDS             complement(356..706)
                     /gene="folB"
                     /locus_tag="PK345_08375"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019246202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HPX35068.1"
                     /translation="MAQIALENMEFYAYHGCFSEEQIIGTHFIIDLFIEYDTTEAEVT
                     DRLAHTLNYQDVFLLVKEQMAIKSNLLEHVGKRILVSLKEHFPEIISAKVKVSKMNPP
                     LGGKIGSVSLLLEI"
     gene            complement(710..>2212)
                     /gene="gltX"
                     /locus_tag="PK345_08380"
     CDS             complement(710..>2212)
                     /gene="gltX"
                     /locus_tag="PK345_08380"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006841687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HPX35069.1"
                     /translation="VRFAPSPTGPLHIGGVRTALYNYLFAKRNNGTFILRIEDTDQLR
                     YVPGAEEYIIESLKWCGIRYDEGPGIGGDYGPYRQSERQNLYRDFANQLINNGYAYYA
                     FDTPQELEEMRERLKAEKSDSQQYDVKTRGGMTNSLTLSQDETQRRISSGEPYVIRIK
                     IPENEIIVVNDLIRGKVEVRSELLDDKVLFKSDGMPTYHLANIVDDHLMDISHVIRGE
                     EWLPSAPLHVFLYRCFKWEAPQFAHLPLLLKPDGNGKLSKRDGDRLGFPVFPLKWEDP
                     ISRDVSSGYRESGYFPEAFINMLALLGWNPGTEQEIFSLEELTKEFSLERVGKSGSKF
                     DPDKAKWFNHKYLVEISNTKLAEQFAPYIAERGITVDLGFVEKVVEIIKERANFVSDF
                     WEQSSFFFTAPQDYDEQVVKKRWKTDSALLMIDLKKQFLLIDDFSAENIKDRIHFFAQ
                     ERNVGLGAIMNALRLLLVGGSFGPDLAVIAEMIGKDEVLKRIDTGILKLG"
BASE COUNT          630 a          490 c          376 g          716 t
ORIGIN      
        1 ttttcaggca tagcatggct ttaagccata ctatgacttt gttattaatt ttccggttta
       61 ctccacaccc gctttggctt ttaaacttaa actgatgact tatactcctt cgcggaaagt
      121 gagagttgtt aggattaact aattatggtt tcttctctat tagtctgttt tttatcaaca
      181 tcatttaggc aatcggggca aaggcaggag gagtattgta cttttaaata attgagcgtt
      241 tctaatttaa ctgttgcttt actgcaccaa caaacaccgg cagggttgca taaaaaaatt
      301 ccgccgcatc tcggacaaac tgatatttgt tgcttttgtg atctcttcat atttgttaaa
      361 tttcaagaag cagacttaca ctcccaatct taccaccaag tggaggattc attttagaca
      421 ctttcacttt tgctgaaatt atttcgggaa aatgttcttt caaacttaca agtatcctct
      481 tgccaacatg ttccaacaaa ttcgatttaa ttgccatttg ttcttttact aaaagaaaaa
      541 catcttgata atttaaagta tgcgcaagac gatcagttac ttccgcctcc gttgtatcgt
      601 attcaataaa aagatcaatg ataaaatgtg ttcctattat ctgctcctca ctaaagcagc
      661 cgtgataagc ataaaactcc atgttttcca atgcgatctg cgccatgatc tatcccaatt
      721 ttaaaattcc ggtatctatt ctttttaaca cttcatcttt gcctatcatt tcagcaataa
      781 cggccaagtc gggcccaaaa ctgccaccta caagcaataa tcgcagcgca ttcattatcg
      841 cacccagacc aacatttctt tcttgtgcga aaaaatgtat ccgatctttg atattttcgg
      901 ctgaaaaatc atcaattaac aagaattgtt tttttaaatc aatcattaaa agtgcggaat
      961 cggttttcca acgctttttc acaacctgtt catcataatc ttgcggagct gtaaagaaaa
     1021 acgatgactg ctcccaaaag tcgctaacaa aattggctct ttcttttatt atctctacaa
     1081 ctttttcgac aaatcccaaa tctactgtga ttcctctttc ggcaatatat ggggcaaatt
     1141 gttcggcgag tttggtattg ctaatctcaa ccaaatattt atggttaaac catttagcct
     1201 tatcagggtc aaatttcgat cccgacttcc ctaccctttc taatgaaaac tcttttgtta
     1261 actcttcaag tgaaaaaatc tcttgttctg ttccgggatt ccaacccaat aaagcaagca
     1321 tgttaatgaa tgcttcaggg aaatatccag attcgcgata cccacttgaa acatctcttg
     1381 agattgggtc ttcccatttc agcggaaaga ccggaaatcc taatctgtcg ccatcacgtt
     1441 tgctaagttt accatttcca tcgggtttaa gaagtaaagg taagtgggca aattgtgggg
     1501 cttcccattt aaaacagcga tatagaaata cgtgtagcgg cgccgaaggc agccattctt
     1561 cacctcttat tacgtgactg atatccatca aatgatcatc aacgatatta gcaagatgat
     1621 aggtaggcat accatcggat ttaaataaaa ccttatcatc aagcagctct gacctaactt
     1681 caacttttcc acgaatcaga tcatttacta ctataatttc gttttccggt attttgattc
     1741 tgatcacata cggctcgccc gaggaaatgc ggcgttgggt ttcgtcctga cttaatgtta
     1801 atgagttagt catacctcct ctggttttta catcgtactg ctggctatca gatttttcag
     1861 ctttcaggcg ttctcgcatt tcctcaagct cttgtggtgt atcaaaagca tagtaggcat
     1921 agccattgtt tataagctgg tttgcaaaat cacgatacag gttttgacgt tccgattgcc
     1981 gatacgggcc ataatctccg cctatgccag gcccttcatc ataacgaata ccacaccatt
     2041 ttaacgattc tattatatac tcttcagcgc ctggtacata cctcagttga tcggtatctt
     2101 caatcctaag gataaaagta ccgttgttcc gtttggcaaa cagataattg taaagcgctg
     2161 tcctgactcc accaatatga agcggaccgg ttgggcttgg agcaaaacgt ac
//
LOCUS       DAOOAI010000144         2145 bp    DNA     linear   ENV 10-JUN-2024
DEFINITION  MAG TPA_asm: Bacteroidales bacterium isolate AMR_MDS_4074
            NODE_56514_length_2145_cov_4.141148, whole genome shotgun sequence.
ACCESSION   DAOOAI010000144 DAOOAI010000000
VERSION     DAOOAI010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN32866045
            Sequence Read Archive: SRR5007348
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Bacteroidales bacterium (wastewater metagenome)
  ORGANISM  Bacteroidales bacterium
            Bacteria; Bacteroidota; Bacteroidia; Bacteroidales.
REFERENCE   1  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2145)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (23-JAN-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/02/2023 06:53:40
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,679
            CDSs (total)                      :: 1,650
            Genes (coding)                    :: 1,640
            CDSs (with protein)               :: 1,640
            Genes (RNA)                       :: 29
            tRNAs                             :: 26
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 10
            CDSs (without protein)            :: 10
            Pseudo Genes (ambiguous residues) :: 0 of 10
            Pseudo Genes (frameshifted)       :: 2 of 10
            Pseudo Genes (incomplete)         :: 8 of 10
            Pseudo Genes (internal stop)      :: 0 of 10
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2145
                     /organism="Bacteroidales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56514_length_2145_cov_4.141148"
                     /isolate="AMR_MDS_4074"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2030927"
                     /environmental_sample
                     /geo_loc_name="USA"
                     /lat_lon="42.35 N 70.96 W"
                     /collection_date="2015-03-03"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            31..210
                     /gene="rpmD"
                     /locus_tag="PK345_08385"
     CDS             31..210
                     /gene="rpmD"
                     /locus_tag="PK345_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007838934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HPX35070.1"
                     /translation="MSKIKIKLVRSGIGRTKGQKLTLQALGLTKMNKVVEHESTPQIL
                     GMVEKVKHLLEVEKL"
     gene            234..680
                     /gene="rplO"
                     /locus_tag="PK345_08390"
     CDS             234..680
                     /gene="rplO"
                     /locus_tag="PK345_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018667878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HPX35071.1"
                     /translation="MDLSNLTPAKGSVKSRKRIGRGEGSGRGGTSTKGHKGAKSRSGY
                     KSKAGFEGGQMPLYRRVPKGGFKNINRIEYRGINLDALQKLVDEKNINVFNLEVLIDH
                     GLAQKKDLIKVLGRGKLKSAIQVTVHAFSKTAQQAIEDKGGVATKI"
     gene            687..2039
                     /gene="secY"
                     /locus_tag="PK345_08395"
     CDS             687..2039
                     /gene="secY"
                     /locus_tag="PK345_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005929061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HPX35072.1"
                     /translation="MKRFIETIKNIYKIEDLRTRIAYTIGIILLYRLGSFVVLPGVDP
                     AMLSALQQQASSGLLGLLNMFSGGAFSNASIFALGIMPYISASIVVQLLGMAIPYFQR
                     LQKEGESGRKKINQITRWLTVIILIFQSPAYIANLVINLPPEAIYPFDPTTTSHSIFS
                     FFGISSIIMLTAGSMFIMWLGEKITDKGIGNGISLIIMIGIIARLPFALFAEFVSRLE
                     EQGGGLIIFIVEIIVLVGVILLTILLVQGTRRIPVQYAKRVVGNKQYGGVRQYIPLKV
                     NAAGVMPIIFAQAIMFLPLTIAQYSQSEALTGFASAFTNYTGFWYNFTFFIMIVAFTY
                     FYTAITVNPNQMADDMKKNGGFIPGIKPGKKTAEFIDNVMSRITFPGSIFLGLVAIMP
                     AFVGIAGVSQQFAGFYGGTSLLILVGVVLDTLDQINSHLLMRHYDGLTKSGRIKGRSG
                     "
BASE COUNT          672 a          362 c          470 g          641 t
ORIGIN      
        1 aaagtgttta atggttaaaa gtagcagaaa atgagcaaga taaaaataaa attggtgaga
       61 agcggtatag gccggacgaa aggtcaaaaa ttgactttac aggcccttgg ccttactaag
      121 atgaataaag tagtagaaca cgaatcaacc cctcagatat taggaatggt tgaaaaagtg
      181 aaacatttat tggaggttga aaaattataa actttgaaaa tagtttaata atgatggatt
      241 tatcaaatct aacacctgct aaaggttcag taaaaagcag aaaaagaata ggccgaggtg
      301 agggatcggg tagaggcgga acttctacaa aaggccacaa aggcgctaaa tcaaggtctg
      361 gctataagtc aaaagcaggc ttcgagggtg gacagatgcc attgtatcgc agagtcccca
      421 aaggcggttt caaaaacata aatcggatag agtatcgagg aattaacctt gatgcactgc
      481 aaaagcttgt tgacgaaaaa aatataaatg tgtttaatct tgaagtccta attgatcatg
      541 gccttgccca aaagaaggat ctgatcaaag tattgggtag aggcaagcta aagtcagcca
      601 ttcaagttac agtacatgct ttttccaaaa cagcgcaaca agccatcgaa gataaaggag
      661 gcgttgcaac aaaaatttaa ttctgaatga aaagatttat agagaccata aaaaacattt
      721 acaagatcga ggaccttaga actcgaattg cctatactat aggaattatt cttctctatc
      781 gtcttgggtc atttgttgtg ctgccgggag ttgatccggc aatgcttagc gcccttcaac
      841 agcaggcgtc atctgggttg ttgggtttgt taaacatgtt ttcgggagga gctttttcca
      901 atgcctctat ctttgcattg ggaatcatgc catacatctc cgcgtccatc gtagttcagt
      961 tgttagggat ggcaataccc tattttcaac gattgcaaaa agagggtgaa agcggacgta
     1021 agaagataaa tcagatcaca agatggctta cagttattat tttgatattc cagtcgcctg
     1081 cctatattgc caatttggta attaatctcc caccggaagc aatttatcca ttcgatccaa
     1141 ctactacatc tcattcgata ttctcgtttt tcggtatttc ttcaataatt atgcttaccg
     1201 cgggatcaat gtttattatg tggcttggtg agaagataac agacaaagga attggcaatg
     1261 gtatttcgct tatcattatg attggtatta ttgccagact gccatttgca ctgtttgctg
     1321 agtttgtttc aagattagag gagcagggcg gcggtttaat tatcttcatc gttgagataa
     1381 tcgttctcgt aggagttatt cttcttacaa tccttttggt acagggaaca cgaagaatac
     1441 ctgtacaata tgcaaaacga gttgttggca ataagcaata tggtggggta aggcagtata
     1501 ttccattaaa ggtaaatgct gcaggcgtta tgcctatcat ttttgcgcaa gcaattatgt
     1561 ttcttccact caccattgca cagtattcac aaagtgaagc tcttacaggc tttgcttcgg
     1621 catttacaaa ttataccggg ttttggtata attttacctt ttttataatg atcgttgcct
     1681 ttacttattt ttatactgcc atcactgtta atcctaatca gatggcggat gatatgaaga
     1741 aaaacggagg ttttattccc ggtattaagc cagggaagaa aactgctgaa tttattgata
     1801 acgtaatgtc gcgaattacc ttcccgggct caattttttt gggattggta gctatcatgc
     1861 cggcatttgt tggtattgct ggtgtaagcc agcagtttgc aggattttac ggaggtacct
     1921 ctctattgat tttggtggga gtggtacttg atacattaga tcaaataaac agccatttgt
     1981 taatgcgtca ctatgacgga ttaacaaaat caggccgaat taaagggaga tcaggataat
     2041 aaactgaaat ccctgaaaaa aatattgata atggttctta ttaaatctga agaggagatt
     2101 gaattaatac gagaaagttc tttgcttgtt ggtaaaacgc ttgcg
//
